Judul: Analisis Keragaman Genetik Ikan Baung (Hemibagrus nemurus) dengan Menggunakan Teknik PCR-RFLP pada DNA Mitokondria
Abstrak: This research has an objective to analyze the genetic variation among four Hemibagrus nemurus population by using PCR-RFLP of D-loop region of mitochondrial DNA. H. nemurus were collected from Cirata, Serayu, Jambi and Bogor. Four restriction enzymes were used to digest D-loop region mt-DNA, i.e. MboI, HaeIII, AluI and RsaI. Based on this analysis, these four population have seven composite haplotypes. The highest haplotype diversity was found at the population from Jambi. Population from Serayu has just one composite haplotype indicating that this population has the same ancestor. Population from Cirata and from Bogor has the same composite haplotype indicating that there was probably migration between these two populations.
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Judul: Studi keragaman genetika populasi udang galah, Macrobrachiumm rosenbergii berdasarkan Polimorfisme Mitokondria DNA
Abstrak: Indonesia dikenal sebagai pusat keberadaan spesies asli udang galah karena masih terdapat 19 spesies asli Indonesia. Udang galah Macrobrachium rosenbergii merupakan salah satu jenis udang Palaemonid air tawar yang umum dibudidayakan di Indonesia. Di alam bebas udang galah sering ditangkap di sekitar sungai, danau, dan rawa - rawa. Di Indonesia udang galah banyak terdapat di wilayah Pulau Sumatra, Pulau Kalimantan, sedangkan di Pulau Jawa, Sulawesi, dan Papua hanya sedikit ditemukan. Keragaman populasi genetik sangat penting untuk menjaga populasi, yang akan memberikan respon paralel terhadap perubahan parameter-parameter lingkungan. Pengamatan variasi atau keragaman genetik dapat dilakukan dengan dua cara yaitu melalui keragaman ale! (allelic diversity) dan heterozigositas. Estimasi keragaman genetik dapat dilakukan dengan menggunakan penanda­penanda molekuler. Salah satu penanda molekuler yang umum digunakan dalam bidang manajemen dan budidaya perikanan adalah penanda DNA mitokondria (mtDNA). Sekuens mtDNA CO-I udang galah yang diperoleh dari hasil amplifikasi memiliki panjang susunan basa antara 700-1500 bp. Dari tujuh enzim restriksi yang digunakan untuk memotong sekuens tersebut (Hae III, Rsa I, Mbo I, Taq I, Alu I, Sac I, dan Hin6 I), hanya terdapat empat enzim yang memberikan situs pemotongan pada sekuens mtDNA CO-I. Lokasi pengambilan koleksi udang galah akan mempengaruhi tingkat variasi haplotype dari tipe restriksi sekuens mtDNA CO-I udang galah.
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Judul: Pembuatan Film Tipis Cu2ZnSnS4 sebagai Lapisan Penyerap Cahaya pada Sel Surya Menggunakan Metode Elektrodeposisi
Abstrak: Thin film of Cu2ZnSnS4 (CZTS) is an alternative semiconductor used as light absorber layer on solar cell. CZTS can replace silicon that commonly used as commercial semiconductor. The advantage of using CZTS as absorber layer is due to the abundance of its chemical composer on earth, and its high value of absorption coefficient. The absorber layer was made by using electrodeposition method on ITO glass substrates. The films were deposited at room temperature using potentiostatic mode with a potential of –1.05 V for 30, 45, and 60 minutes. After that, the films were annealed at two different temperatures, 180 ºC and 500 °C, for 45 minutes in N2/H2S atmosphere. Based on X-ray diffraction analysis, the polycrystalline thin film obtained fall into kesterite crystal phase. This type of crystal was obtained when the CZTS was annealed at 500 °C in H2S/N2 atmosphere. The value of optical band gap energy for the CZTS thin film was 2.2 eV with the film thickness about 1.228–6.366 μm. The sample of CZTS with electrodeposition time of 45 minutes and annealing temperature of 500 °C showed the best result and in conformity with absorber layers criteria for solar cells.
Keyword: semiconductor, ITO glass, CZTS thin film, electrodeposition