Judul: Landscape Services Management of Water Resource and Thermal Comfort of City Park in Jakarta
Abstrak: Jakarta terus mengalami peningkatan kepadatan kota yang menyebabkan Ruang Terbuka Hijau (RTH) berkurang. Dampak yang terjadi adalah penurunan kualitas lanskap dan kenyamanan hidup karena adanya keterbatasan resapan air dan peningkatan suhu. Hal tersebut dibuktikan oleh Indeks Kualitas Lingkungan Hidup Jakarta 2022 yang berada pada kategori sedang. Taman kota mampu memberikan kontribusi yang signifikan sebagai penyedia jasa lanskap dalam menjaga keseimbangan siklus air dan mengatur iklim mikro. Penelitian ini bertujuan menganalisis jasa lanskap pengaturan tata air dan kenyamanan termal pada Taman Bambu, Taman Cattleya dan Taman Langsat, lalu menyusun rekomendasi pengelolaan lanskapnya. Perangkat i-Tree Eco digunakan untuk analisis jasa lanskap tata air menggunakan plot sampel. Temperature Humidity Index (THI) dilakukan untuk mengetahui kategori kenyamanan melalui pengukuran suhu dan kelembapan. Penyusunan rekomendasi strategi pengelolaan jasa lanskap dilakukan dengan analisis Strengths, Weaknesses, Opportunities, and Threats (SWOT). Hasil analisis i-Tree Eco menunjukkan pohon di ketiga taman kota mampu mengurangi limpasan 51,28 m³/tahun dengan nilai yang diperkirakan Rp1.944.573,61. Hasil analisis THI menunjukkan Taman Bambu memiliki THI 30, Taman Cattleya memiliki THI 30,4, dan Taman Langsat memiliki THI 30,2, ketiganya menunjukkan kategori kenyamanan yang tidak nyaman. Berdasarkan hasil analisis SWOT, ketiga taman kota berada pada Kuadran V, yakni pengelolaanya harus disusun berdasarkan hold and maintain strategy., Jakarta continues to experience increasing urban density, leading to a reduction in Green Open Spaces (GOS). This has decreased landscape quality and living comfort due to limited water infiltration and rising temperatures, evidenced by Jakarta's 2022 Environmental Quality Index, which is categorized as moderate. City parks play a significant role in provisioning landscape services by maintaining water cycles and regulating microclimates. This study aims to analyze the landscape services related to water resource and thermal comfort in Taman Bambu, Taman Cattleya, and Taman Langsat, and formulate recommendations for its landscape management. The i-Tree Eco was used to analyze water resource service using sample plots. The Temperature Humidity Index (THI) was used to determine the comfort category through temperature and humidity measurements. Recommendations for landscape management strategies were formulated using Strengths, Weaknesses, Opportunities, and Threats (SWOT) analysis. The i-Tree Eco analysis results show that trees in the three city parks can reduce runoff by 51,28 m³/year, with an estimated value of IDR 1.944.573,61. The THI analysis results show that Taman Bambu has a THI of 30, Taman Cattleya has a THI of 30,4, and Taman Langsat has a THI of 30,2, all indicating an uncomfortable category. Based on the SWOT analysis results, the three city parks are in Quadrant V, suggesting that their management should be developed based on a hold and maintain strategy.
Keyword: I-Tree Eco, jasa lanskap, manajemen lanskap, taman kota, temperature humidity index

Judul: Landscape Services Management of Biodiversity and Carbon Stock City Park in Jakarta
Abstrak: Perkembangan wilayah perkotaan, terutama di DKI Jakarta, memberikan dampak positif dan negatif pada kualitas lanskap. Peningkatan polusi udara merupakan salah satu bentuk dampak negatif pada kualitas lanskap. Perkotaan seringkali menjadi sumber polusi udara tinggi, terutama dari pusat industri. Oleh karena itu, penting untuk memahami peran Ruang Terbuka Hijau (RTH), khususnya taman kota, dalam memperbaiki kualitas lanskap. RTH memiliki fungsi ekologi untuk mereduksi polusi udara, memberikan kontribusi pada keberlanjutan kota. Penelitian ini bertujuan untuk menganalisis jasa lanskap berupa biodiversitas dan karbon tersimpan menggunakan i-Tree Eco dan menyusun rekomendasi pengelolaan berdasarkan analisis SWOT. Analisis jasa lanskap biodiversitas diklasifikan dengan indeks Shannon-Wiener, sementara analisis cadangan karbon juga diklasifikasikan berdasarkan kriteria jumlah karbon tersimpan. Berdasarkan analisis Indeks Shannon-Wiener, Indeks keanekaragaman pada Taman Suropati dengan nilai 1,30 termasuk kategori keberagaman sedang, Taman Menteng dengan nilai 3,21 termasuk kategori keberagaman tinggi dan Taman Puring dengan nilai 1,34 termasuk kategori keberagaman sedang. Berdasarkan hasil analisis i-Tree Eco karbon tersimpan pada Taman Suropati sebesar 114,11 (ton/ha) termasuk kategori karbon tersimpan tinggi, pada Taman Menteng sebesar 41,7 (ton/ha) termasuk kategori karbon tersimpan rendah dan untuk karbon tersimpan pada Taman Suropati sebesar 56,4 (ton/ha) termasuk kategori karbon tersimpan sedang. Pada hasil analisis SWOT menunjukan bahwa skor IFE dan EFE berada pada posisi kuadran I (Grow and build strategy) strategi ini berfokus pada pengelolaan dan pengembangan lanskap. Berdasarkan hal tersebut, diperoleh strategi-strategi pengelolaan yang dapat diterapkan pada Taman Suropati, Taman Menteng dan Taman Puring diantaranya, meningkatkan biodiversitas dan karbon tersimpan dengan penanaman dan penyulaman, memberikan edukasi kepada masyarakat terkait kesadaran akan pentingnya menjaga kebersihan taman kota dan melakukan pengawasan terhadap pemeliharan rutin fasilitas dan vegetasi yang ada di Taman Kota., The development of urban areas, especially in DKI Jakarta, has both positive and negative impacts on landscape quality. One of the negative impacts is the increase in air pollution. Urban areas often become sources of high air pollution, particularly from industrial centers. Therefore, it is important to understand the role of Green Open Spaces, especially city parks, in improving landscape quality. Green Open Spaces has an ecological function to reduce air pollution and contributes to urban sustainability. This study aims to analyze landscape services in terms of biodiversity and stock carbon using i-Tree Eco and to develop management recommendations based on SWOT analysis. The analysis of biodiversity landscape services is classified using the Shannon-Wiener index, while the analysis of carbon stock is classified based on the amount of stocked carbon. According to the Shannon-Wiener Index analysis, the biodiversity index in Suropati Park with a value of 1,30 is categorized as medium diversity, Menteng Park with a value of 3,21 is categorized as high diversity, and Puring Park with a value of 1,34 is categorized as medium diversity. Based on the I-Tree Eco analysis, the carbon stock in Suropati Park is 114,11 (tons/ha), categorized as high stock carbon, in Menteng Park it is 41,7 (tons/ha), categorized as low stock carbon, and in Puring Park it is 56,4 (tons/ha), categorized as medium stock carbon. The SWOT analysis results show that the IFE and EFE scores are in quadrant I (Grow and build strategy), focusing on
Keyword: I-Tree Eco, jasa lanskap, manajemen lanskap, ruang terbuka hijau, taman kota

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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