Judul: In Vitro Stress Treatment On Axillary Shoots of Mucuna bracteata to Increase Shoot Number
Abstrak: Mucuna bracteata is a plant that is very effectively used as a legume cover crop (LCC), which is a cover crop plant that have a function to suppress weeds growth. Productivity of M. bracteata shoots in tissue culture have an effect in the plantation industry. However, at a certain subculture from the multiplication medium to the next subculture, there is a decline in shoot growth of M. bracteata. This study aims is to increase the number of shoots of M. Bracteata with stress methods of culture induction of shoots. Medium stress treatments performed for 14 days in the dark and bright with the composition of medium MS macro 0-25% (A), MS macro 25-50% (B), MS macro 50-75% (C), sucrose 50-75% (D), and vitamins 50-75% (E). After observation for two months, the number of shoots is same between sample with the media's treatment of (A), (B), and (E), even the number is decreasing 100%. That is unlike the (C) which the number of shoots is increase. Similar thing occurs in (D) treatment, although it is not as good as (C). Differences of storage space affect the morphology of the explants. Explants were incubated in the dark room similar to the sprouts, while the explants were incubated in the light have a morphology that similar to the original plant. This shows that the best media of stress treatment used is a composition of MS macro 0-25% (C) in bright conditions.
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Judul: Multiplikasi Tunas Suweg (Amorphophallus paeoniifolius (Dennst.) Nicolson) dengan Zat Pengatur Tumbuh BAP dan NAA secara Kultur Jaringan
Abstrak: The utilization of suweg (Amorphophallus paeoniifolius) in Indonesia has been known since the Japanese colonial period as source of carbohydrate. Suweg is beneficial for diabetes, digestion, liver, and increase appetite. The cultivation of suweg is by planting its eye shoot of tuber’s skin, the main eye shoot of tuber, the whole tuber, sapling from the tuber, and seedling. But, there are some problems with that technique which are the habitat where it grows, planting time, and the quality of the result is not uniform. So another cultivation effort is needed which is tissue culture. Based on Isnaini et al. (2012), tissue culture of suweg produces few shoots so it needs further study in order to produce many shoots in a callus. This research was done by the addition of Plant Growth Regulators (PGR) NAA and BAP that is potential for shoot growth. The aim of this study is to determine the effect of BAP and NAA in suweg shoot multiplication. This research was conducted at the Laboratory of Plant Tissue Culture Conservation Centre Bogor Botanical Garden, from June until September 2012. The research was designed by using a two-factor factorial experimental design on the basis of completely randomized design (CRD). The first factor is the concentration of BAP with four levels which are 0 mg/l, 1 mg/l, 2 mg/l, and 3 mg/l. The second factor is the NAA concentrations with four levels which are 0 mg/l, 0.25 mg/l, 0.5 mg/l, and 0.75 mg/l. Observed was done to explants browning, contamination (bacteria and fungi), callus color, callus texture, number of shoot, and the number of roots. The result shows the percentage of successful explant survival is 94.17% or 226 explants. Contamination by bacteria and fungi are 2.08 or 5 explants, and browning are 3.75% or 9 explants. Explant with compact texture is 34.58% and explant with crumb texture is 63.33%. The most root growth was found in the treatment of 1 mg /1 BAP + 0.75 mg/l NAA is 2.87 root/ week. The highest number of shoot in the treatment of 1 mg/l BAP + 0.25 mg/l NAA is 0,47 shoot/ week. The conclusion of this study is that the shoot multiplication was occurred in three explants, which are in the treatment of 1 mg l BAP + 0.25 mg/l NAA, 3 m/l BAP + 0.5 mg/l NAA and 3 mg/l BAP + 0.75 mg/l NAA.
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Judul: Infeksi Virus Parainfluenza Pada Anjing
Abstrak: Virus parainfluenza anjing termasuk famili Paramyxoviridae, berbentuk pleomorf dengan ukuran bervariasi (bergaris tengah antara 100-500 nm), berpembungkus peka ether, berisi genom RNA dan secara serologis mempunyai kesamaan dengan virus SV-5 kera; dikenak sebagai salah satu kausa penting dari sindrom "kennel cough". Infeksinya dapat mengenai anjing semua umur yang belum pernah terinfeksi atau mendapatkan vaksinasi mengakibatkan gejala klinis ringan yang terbatas pada traktus respirasi atu infeksi yang secara klinis tidak memperlihatkan gejala nyata. Gejala klinis yang nampak setelah masa inkubasi (1-5 hari) terlewati berupa batuk yang tidak berat selama beberapa hari, berbagai tingkat tonsilitis dan pharyngitis dan kadang-kadang ditemukan kenaikan temperatur rektal antara 1-2 derajat C dari temperatur normal rat-rata. Anjing terinfeksi mengandung virus pada traktus respirasi bagian atas dan bawah serta limfonoduli sampai hari ke 9 setelah infeksi. Penyebaran virus antar anjing terjadi secara aerogen.
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