Judul: Pengaruh Kelekatan Teman Sebaya terhadap Konsep Diri dan Kecerdasan Emosi Remaja di Panti Asuhan di Kota Banda Aceh
Abstrak: Berbagai masalah sosial seperti kemiskinan, kehilangan orang tua akibat kematian, dan ketiadaan keberadaan orang tua yang disebabkan konflik serta bencana alam seringkali berdampak terhadap keberadaan anak-anak terlantar termasuk yang tinggal di panti asuhan. Anak-anak yang kemudian tinggal di panti asuhan tentu saja akan mempunyai lingkungan sosial baru dan akan memengaruhi kehidupannya. Oleh sebab itu, penelitian ini bertujuan untuk menemukan pengaruh karakteristik remaja, karakteristik keluarga, karakteristik kelompok teman sebaya, dan kelekatan teman sebaya terhadap konsep diri dan kecerdasan emosi remaja di panti asuhan. Penelitian dilakukan di dua panti asuhan di Kota Banda Aceh dengan penarikan contoh secara purposif, yaitu remaja di panti asuhan terpilih dengan usia 12-18 tahun. Data diperoleh dengan metode wawancara menggunakan kuesioner terstruktur kepada 63 remaja. Sesuai dengan hipotesa penelitian, hasil penelitian menunjukkan bahwa kelekatan teman sebaya memiliki hubungan positif signifikan dengan konsep diri dan kecerdasan emosi sedangkan konsep diri berhubungan positif sangat signifikan dengan kecerdasan emosi remaja di panti asuhan. Hasil uji regresi menunjukkan bahwa terdapat pengaruh positif sangat signifikan kelekatan teman sebaya terhadap konsep diri maupun terhadap kecerdasan emosi. Uji regresi tidak menemukan pengaruh yang lebih nyata pada variabel konsep diri terhadap kecerdasan emosi remaja di panti asuhan.
Keyword: kecerdasan emosi, kelekatan teman sebaya, konsep diri, panti asuhan, remaja

Judul: Pengaruh Interaksi dengan Pengasuh dan Penerimaan Teman Sebaya terhadap Self-Esteem Remaja di Panti Asuhan.
Abstrak: Penelitian ini bertujuan untuk menganalisis pengaruh interaksi dengan pengasuh dan penerimaan teman sebaya terhadap self-esteem remaja di panti asuhan. Penelitian dilaksanakan di empat panti asuhan di Kota Bogor yang dipilih berdasarkan rekomendasi dari Dinas Sosial. Penelitian ini menggunakan desain cross-sectional study dengan melibatkan 115 remaja, berusia 13-20 tahun yang tinggal di empat panti asuhan terpilih. Contoh dipilih secara sensus. Pengambilan data dilakukan dengan teknik self-administered. Hasil penelitian menunjukkan bahwa terdapat hubungan positif signifikan antara interaksi dengan pengasuh dan penerimaan teman sebaya; juga memiliki hubungan positif signifikan antara penerimaan teman sebaya dan self-esteem. Hasil uji regresi menunjukkan penerimaan teman sebaya memiliki pengaruh positif signifikan terhadap selfesteem remaja.
Keyword: panti asuhan, penerimaan teman sebaya, interaksi dengan pengasuh, remaja, self-esteem

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable