Judul: Pengaruh Waktu Tebang Terhadap Kekuatan Lentur Bambu Andong (Gigantochloa Pseudoarundinaceae (Steudel) Widjaja).
Abstrak: Pemanenan atau penebangan bambu andong secara tradisional dilakukan pada waktu–waktu tertentu, yaitu umumnya pada awal musim kemarau. Faktor–faktor lingkungan antara lain suhu, kelembaban, curah hujan, dan intensitas cahaya berpengaruh penting pada metabolisme bambu sehingga mempengaruhi kekuatan lentur bambu. Penelitian ini bertujuan untuk mengetahui pengaruh waktu tebang terhadap kekuatan lentur bambu andong serta menentukan faktor lingkungan yang paling berpengaruh terhadap kekuatan lentur bambu andong. Pada penelitian ini, pemanenan dilakukan selama satu tahun, yaitu satu batang bambu ditebang setiap akhir bulan yang dimulai Februari 2013 sampai Januari 2014. Bambu yang diambil setiap bulan dibuat contoh uji sesuai standar ASTM D 143-94 untuk diuji kekuatan lenturnya. Hasil penelitian menunjukkan bahwa MOE dan MOR tertinggi diperoleh pada bambu yang dipanen bulan Maret. Berkaitan dengan hal tersebut, jika diinginkan kekuatan tertinggi disarankan untuk memanen bambu andong pada bulan Maret. Kekuatan alami bambu terutama dipengaruhi intensitas cahaya, suhu, dan kelembaban, sedangkan curah hujan tidak berpengaruh nyata.
Keyword: bambu andong, kekuatan lentur, waktu tebang

Judul: Pengaruh Waktu Tebang terhadap Keawetan Alami Bambu Andong (Gigantochloa psedoarundinace)
Abstrak: Gigantochloa pseduarundinaceae, also known as Andong bamboo, used in this study. Samples were taken from bamboos which were harvested once each month in a year starting from February 2013 to January 2014. Bamboos culms were cut into strips. The strips were put in a safe, dry place, and free from wood destroying organisms. After all of the bamboos were collected, the laboratory testing is conducted based on SNI 01.7207-2006 methods. Field testing were also conducted based on ASTM D 1758-06 methods. According to the laboratory test, the bamboos were categorized as durability class III (medium durability) and durability class IV (bad durability). According to laboratory testing, the least loss of bamboo mass occurred on Maret and the highest number of termite mortality occurred on November. While, the results of the field test show that the value of the highest durability grade and the weight lowest loss rate was occurred on bamboo which is harvested in March to May. Best subset regression were used to determine the environmental factors during harvesting process which was dominantly affecting the bamboos' durability. The observed environmental factors were rain levels, humidity, and temperature. According to best subset regression analysis, the rain level and humidity during the harvesting period were two main factors affecting the bamboos' natural durability. The best subset regression resulted that rainfall and humidity during the harvest were the two main factors that affect the durability of bamboo at observed daily rainfall intervals (1-20 mm). Rain levels and humidity give fluctuating effects on the loss of mass. The maximum loss was happened on 74% humidity. If the humidity is bellow 74%, or above 74%, the mass loss tend to decrease. The rain level also affect the mass loss. Low rainfall level 1 to 4 mm showed the decrease in mass loss, and became stabilized when the rain level reach above 4 mm. According to the data, it is recommended to harvest the bamboos on March to Mei, when the durability are the highest and the mass loss are the lowest.
Keyword: Bogor Agricultural University (IPB), resistance class, natural durabiliy, mass loss, harvesting time, environmental factors

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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