Judul: Keragaman Genetik Ikan Kiper (Scatophagus argus) Berdasarkan  Marka Gen 16s rRNA di WPP 573 Bagian Barat sebagai Dasar Pengelolaan
Abstrak: Ikan kiper (Scatophagus argus) merupakan salah satu ikan demersal yang memiliki nilai ekonomis penting, ikan ini dijadikan sebagai ikan konsumsi dan ikan hias. Ikan kiper juga merupakan ikan yang aktif bermigrasi sehingga memungkinkan adanya perpindahan genetik dari perairan yang berbeda. Penelitian ini bertujuan untuk menganalisis keragaman genetik ikan kiper di WPP 573 Bagian Barat berdasarkan marka gen 16s rRNA sebagai pengelolaan sumberdaya yang berkelanjutan. Hasil amplifikasi menunjukkan amplicon dengan pita untaian tunggal namun masih terdapat smear akibat adanya kontaminan. Hasil sekuensing dan validasi BLASTn menunjukkan bawah seluruh contoh sampel ikan kiper termasuk ke dalam spesies Scatophagus argus dengan persen identifikasi sebesar 99,51 sampai dengan100%. Hasil penelitian menunjukkan bahwa antar sampel ikan kiper memiliki hubungan kekerabatan yang sangat dekat, namun populasi ikan kiper tergolong ke dalam kategori tidak baik karena tingkat keragaman yang rendah., Spotted scat (Scatophagus argus) is one of the demersal fish that has important economic value, this fish is used as a consumption fish and ornamental fish. It is also an active migratory fish that allows genetic transfer from different waters. This study aims to analyze the genetic diversity of Spotted scat in WPP 573 West Part based on 16s rRNA gene markers as a sustainable resource management. Amplification results show amplicon with single strand band but there are still smears due to the presence of contaminants. The BLASTn sequencing and validation results showed that all Spotted scat samples belonged to the Scatophagus argus species with a percent identification of 99.51 until100%. The results showed that the Spotted scat samples had a very close kinship relationship, but the population of spotted scat was classified into the unfavorable category because of the low level of diversity.
Keyword: Gen 16s, Genetic diversity, Scatophagus argus

Judul: Molecular identification of the indian mackerel (Rastrelliger  kanagurta) based on 16S rRNA gene markers in FMA NRI 572 and 573 as a basis  for fisheries management
Abstrak: Ikan kembung lelaki (Rastrelliger kanagurta) memiliki nilai ekonomis penting bagi nelayan. Ikan pelagis kecil di WPP-NRI 572 dan WPP-NRI 573 telah mengalami kondisi fully-exploited. Penelitian ini bertujuan memastikan spesies, keragaman genetik dan kekerabatan spesies secara molekuler ikan kembung lelaki (Rastrelliger kanagurta) berdasarkan marka gen 16S rRNA di WPP-NRI 572 dan 573 sebagai dasar pengelolaan perikanan. Penelitian dilakukan pada bulan Juni 2021 hingga Januari 2022. Sampel ikan diambil dari perairan Labuan, Cilacap, dan Banyuwangi dengan jumlah total sebanyak 9 individu. Urutan basa nukleotida hasil sekuensing dianalisis menggunakan software MEGA X. Hasil identifikasi DNA barcoding, menunjukkan spesies ikan kembung dari tiga lokasi berbeda merupakan ikan kembung lelaki (Rastrelliger kanagurta). Nilai keragaman genetik ikan kembung lelaki dari tiga lokasi termasuk dalam kategori tinggi. Hal ini menunjukkan populasi ikan kembung tergolong stok yang baik, The Indian Mackerel has important economic value for fishermen. The small pelagic fisheries in WPP-NRI 572 and WPP-NRI 573 have experienced a fully-exploited condition. This study aims to determine species certainty, genetic diversity and genetic relationships of indian mackerel (Rastrelliger kanagurta) molecularly based on 16S rRNA gene markers in WPP-NRI 572 and 573 as a basis for fisheries management. The research was conducted from June 2021 to January 2022. Fish samples taken from the waters of Labuan, Cilacap, and Banyuwangi with a total of 9 individuals. The nucleotide base sequences of the sequencing results were analyzed using MEGA X software. The identification results of DNA barcoding showed that the mackerel species studied from three different locations were the Indian Mackerel (Rastrelliger kanagurta). The results showed that the genetic diversity value of male mackerel from three locations was included in the high category indicating that the mackerel population was classified as a good stock.
Keyword: 16S rRNA genes, genetic diversity, Rastrelliger kanagurta

Judul: Utilization of terra/MODIS L1B data for analysis of horizontal wind profile in the troposphere
Abstrak: This study was concerned with horizontal wind derived from data collected with the Moderate-resolution Imaging Spectroradiometer (MODIS) on board the NASA’s Earth Observation System (EOS)-Terra satellite. The aim of this study is to develop a method for the estimation of horizontal wind movement in the troposphere based on Navier-Stokes equation. The method was applied to a calibrated and geolocated Terra/MODIS L1B 1 km data set. The data inputs to estimate surface temperature are retrieved from TIR (Thermal Infrared) bands 31 (10.780 μm to 11.280 μm) and 32 (11.770 μm to 12.270 μm). The obtained surface temperatures were later used to approximate geopotential height on a constant-pressure surface by using the modified Poisson equation. The main assumptions used are the hydrostatic equilibrium, ideal gas law, constant lapse rate and constant acceleration of gravity. Based on the distribution of geopotential height, the pressure gradient force is measured by the gradient of geopotential on an isobaric coordinate to present the horizontal wind profile. For certain topographic areas especially in the mountain ranges, there was a disadvantage of using pressure as a vertical coordinate which lead to inaccuracy. In spite of that, Terra/MODIS L1B data with 1 km spatial resolution can be used to estimate the horizontal wind movement in the troposphere.
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