Judul: Karakteristik dan genesis tanah gambut pedalaman yang dipengaruhi oleh luapan sungai barito dan kahayan
Abstrak: Gambut merupakan tanah yang mempunyai karakteristik yang sangat beragam baik secara horizontal maupun vertikal. Salah satu faktor yang mempengaruhi karakteristiknya adalah air sungai. Gambut pedalaman Kalimantan Tengah terletak diantara Sungai Barito dan Kahayan, diketahui memberikan hasil produksi padi dan jagung yang berbeda. Tanah diambil dari gambut pedalaman Kalimantan Tengah, yaitu Mengkatip (yang dipengaruhi oleh luapan Sungai Barito) dan Berengbengkel (yang dipengaruhi oleh luapan Sungai Kahayan). Percobaan penanaman tanaman padi dan jagung dilakuakn dikamar kaca Jurusan Tanah IPB, analisis contoh air, dan analisi contoh tanah meliputi analisis sifat fisik dan kimia tanah di Laboratorium Jurusan Tanah IPB, Bogor, analisis mineralogi liat di PPTM Bandung, analisis mikromorfologi di Laboratorium Fakultas Geografi UGM, Yogyakarta.
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Judul: Karakteristik dan genesis gambut pantai di sekitar S. Kapuas/Barito dan S. Kahayan Kalimantan Tengah
Abstrak: Keragaman faktor yang mempengaruhi proses pembentukan tanah gambut menyebabkan keragaman sifat tanah yang juga tinggi. Proses yang terjadi selama masa pembentukannya sangat mempengaruhi karakteristik tanah yang terbentuk. Dalam proses pematangan dan pembentukan karakteristik tanah selanjutnya, tebal lapisan gambut dan kualitas air yang meluapinya turut berperan. Karena karakteristik tanah mencerminkan tingkat produktifitas tanahnya, maka dalam usaha pengembangannya menjadi lahan pertanian produktif perlu diketahui karakteristik dan genesis tanah gambut serta potensi produksinya sehubungan dengan suplali air sungai yang mempengaruhi proses pembentukannya.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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