Judul: Introduksi gen Hd3a ke dalam tanaman kentang (Solanum tuberosum L.) Kultivar Diamant
Abstrak: Hd3a gene encoded a protein inducing flowering and tuber formation in potato cultivar Andigena. This research has an objective to introduce the Hd3a gene into potato (Solanum tuberosum L.) cultivar Diamant under control of promoter rolC. Potato plants were propagated in vitro on MS2 macro medium. Genetic transformation was carried out by co-cultivation method by using Agrobacterium tumefaciens LBA4404. Internodes were used as explants for transformation. Selection of transgenic plants was performed in MS medium containing 10-40 mg/L hygromycin. The results showed that the average of transformation efficiency was 22.53% and regeneration efficiency of the calli was 100%. Transformation of 18 explants resulted 9 putative transgenic shoots and the regeneration rate was 2.33 putative transgenic shoots per callus. Transformation efficiency was affected by plant genotype, acetosyringone concentration, and technical factors such as washing and pincetting during the transformation process.
Keyword: Bogor Agricultural University (IPB), Solanum tuberosum L.., Hd3a gene, genetic transformation, cultivar Diamant, Agrobacterium tumefaciens

Judul: Transformasi Genetik Kentang (Solanum tuberosum L.) Kultivar Agria dengan Gen Pembungaan Hd3a
Abstrak: Potato is an important vegetables commodity in Indonesia. Agria has a potential cultivar to be used as french fries material. Flowering induction of this cultivar is very important to use this cultivar as a parent in the crossing with other cultivar. The aim of this research was to transform genetically potato cultivar Agria with Hd3a flowering gene controlled by rolC promoter. Potato plants were propagated in vitro on MS2 macro medium. Genetic transformation was carried out by co-cultivation method by using Agrobacterium tumefaciens. Putative transgenic calli and shorts were selected in MS and MS2 macro media respectively containing 10-20 mg/L hygromycin. The results showed that the transformation efficiency was 5.01% with regeneration efficiency relatively high 100%. Putative transgenic shoot regeneration per calli was one. Putative transgenic plants produced tuber in MS2 macro media whereas all non-transgenic plants did not. This result indicated that Hd3a induced formation of tuber of putative transgenic potato cultivar Agria.
Keyword: Bogor Agricultural University (IPB), Solanum tuberosum L., Hd3a flowering gene, genetic transformation, cultivar Agria

Judul: Truncated Stop Loss as a Optimal Solution of Reinsurance Agreement in One-Period Model
Abstrak: Reinsurance is one of risk management efforts of an insurance company, which is called the cedent. Similar with insurance, reinsurance constitutes the cedent to pay the premium. We consider one-period reinsurance model and derive a rule which minimizes the ruin probability of the cedent for a fixed reinsurance premium. Therefore, it is needed to construct an optimal reinsurance agreement for the cedent. The premium is calculated according to four different principles, i.e. economic, generalized zero-utility, Esscher, and mean-variance principles. Stop loss is a reinsurance contract which protect the insurance company for losses that exceed a certain amount. The result of this research shows that a truncated stop loss is an optimal agreement in the class of all reinsurance contracts with various premiums calculated using above mentioned principles.
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