Judul: Evaluasi Saccharomyces cerevisiae Teradaptasi Hidrolisat Kappaphycus alvarezii dalam Kemampuan Mengonversi Hidrolisat Menjadi Bioetanol
Abstrak: Bioetanol is one of renewable energy source that can be obtained by fermentation and is environmental friendly. The production of bioetanol needs two important components that are the substrate and fermentation agent. Kappaphycus alvarezii is a potential subrate to be fermented by Saccharomyces cerevisiae, the most common microorganism for fermentation process. This study aimed at determining the ability of the 9th generation slow adapted S. cerevisiae strain (IPBCC 05.548 AL IX) to convert K. alvarezii hydrolysates into bioetanol. The yeast growth in hydrolysates and Yeast Malt Glucose Pepton (YMGP) was maximum at 42 hour incubation. In the hydrolysates, the cell size was relatively longer and the number of cells with large size were more than those in the YMGP. About 90 mL hydrolysates K. alvarezii that was fermentated with 10 mL of 106 mL-1 inoculum yielded maximum bioetanol 1,94% (v/v) at 168 hours incubation. Its fermentation, and substrate efficiency were 36,57%, and 59,85%, respectively. At the same incubation time and 106 cells mL-1 inoculum, 1,02% (v/v) bioetanol was yielded with fermentation, and substrate efficiency were 21,29%, and 70,07% respectively
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Judul: Adaptation of saccharomyces cerevisiae to cassava acid hydrolisates for bioethanol production.
Abstrak: Acid hydrolisates of cassava contain toxic substances i.e. 3,55 g/l HMF and 0,72 g/l furfural to ethanol producing yeast, Saccharomyces cerevisiae. This study is aiming at screening four strains of S. cerevisiae for their tolerance capacity to that acid hydrolisates. Out of four strains, S. cerevisiae IPBCC 05.548 showed the highest tolerance on the bases of ethanol production. It produced 4,1% (b/v) ethanol. Prior to adaptation, optimum sugar concentration and starter dosage were determined in sequence. Out of 15%, 18%, 20% and 24% concentration. S. cerevisiae IPBCC 05.548 showed the highest ethanol production (4,10% b/v) at 15% of total sugar. Among starter dosage (1, 2 and 3 times of 0,23% of sugar concentration), that were tested on optimal sugar concentration indicated that the twice of dosage was the best for ethanol production. Adaptation was then performed for 72 hours for each cycle. Of the cycles, the 9th cycle (648 hours) showed the highest specific growth rate (0,14g.h-1) as well as ethnol yield (4,13% b/v). Comparison of the adapted strain to unadapted strain proved that the adapted strain produced 30,78% higher ethanol yield than those of unadapted strain.
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Judul: Karakteristik Anatomi Skelet Tubuh Badak Jawa (Rhinoceros Sondaicus).
Abstrak: Badak jawa (Rhinoceros sondaicus) merupakan spesies mamalia besar di dunia yang paling terancam punah dan hanya dapat ditemukan di Taman Nasional Ujung Kulon (TNUK), Provinsi Banten, Indonesia. Tujuan dari penelitian ini adalah mempelajari karakteristik spesifik anatomi skelet tubuh badak jawa. Struktur skelet ini dikaitkan dengan fungsinya untuk menunjang ukuran tubuh yang besar dan perilakunya. Penelitian ini menggunakan satu set preparat skelet tubuh badak jawa. Parameter penelitian yang dilakukan adalah mengamati bentuk bagian skelet yang khas, mengukur panjang dan lebar tulang, serta dibandingkan dengan skelet tubuh badak sumatra dan hewan piara lain. Kemudian, sistem penamaan pada setiap bagian tulang dilakukan berdasarkan Nomina Anatomica Veterinaria (ICVGAN 2012). Karakteristik anatomi skelet tubuh badak jawa relatif mirip pada badak sumatera, tetapi memiliki ukuran yang lebih besar. Terdapat beberapa perbedaan yang ditemukan yaitu, ala atlantis lebih panjang, processus spinosus os axis berbentuk segiempat, processus transversus et spinosus tulang leher lebih subur, processus spinosus os vertebrae thoracicae relatif lebih mengarah ke kaudodorsal terutama pada sembilan ossa vertebrae thoracicae di kranial, daerah flank lebih sempit, dan ossa sacrales yang tersusun lebih kompak dan rigid. Secara umum skelet tubuh badak jawa memiliki hubungan yang sangat erat, kokoh, dan kompak, disertai penjuluran-penjuluran dan aspek kasar sebagai tempat melekatnya otot-otot dan ligamenta dengan kuat. Hal ini berperan dalam mempertahankan rigiditas dan menjaga sikap tubuh badak jawa terkait dengan habitat dan perilakunya.
Keyword: anatomi, badak jawa, skelet tubuh