Judul: Kajian Metode Penggerombolan Dua Tahap untuk Data yang Mengandung Pencilan
Abstrak: Cluster analysis is often encountered in various studies. Analysis of classical clusters, such as hierarchical clustering method and k-means clustering cannot handle categorical variables or a mixture of numerical and categorical. In addition, the determination of the optimal number of clusters are still dependent on the subjectivity of the researcher and cannot handle very large datasets, which is larger than 500. One approach to addressing this problem is to use a two-step clustering method. The accuracy of the two-step clustering method of predicting the number of clusters generated as well as the classification of cluster membership, especially in the data containing outliers is important to be studied. Outliers in the data containing a small (1%), this method provides more accurate compared with the results of data containing a large outliers (5% or 15%). Scale use of outliers handling in the data containing outliers must be greater than the amount of outliers itself. Two-step clustering method is very accurate in producing a number of clusters associated with the actual number of population clusters that do not contain data outliers, especially in the most variable of type numeric and categorical rest. Clustering villages in Indonesia by a factor of progress and backwardness villages using a two-step clustering method generates optimal cluster 7.
Keyword: two-step clustering method, data outliers, cluster analysis

Judul: Penerapan Metodetwo Step Cluster Dalam  Analisis Gerombol  (Studi Kasus : Data Potensi Desa Sensus Ekonomi 2003 Wilayah Jawa Barat)
Abstrak: Clustering is a process of grouping objects based on similarities . Some problems in cluster analysis are the different scale of measurement, large data sets and unspecified number of cluster. One approach to handle this problems is by using Two Step cluster method. The purpose of this research is to applicate Two Step cluster method by clustering villages residing in West Java. The result of Two Step cluster method are pre-cluster which early yielded at first phase counted eight clusters and optimal cluster which yielded at second phase counted three clusters. Members in first cluster are objects which is extreme outliers and cannot be grouped into other clusters. Villages which include into second clusters have rural characteristic. The village has wide farm, many agriculture households, but also has underdeveloped field of industries and also information and communications. So that, to improve village potency for this cluster, the variables which still have low growth level has to be increased. The third cluster has the characteristic of urban status. Village in this cluster has the closest distance to downtown, developed enough in the field of industry, information and communications, but still have the highest number for unemployment citizens.
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Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable