Judul: Bambu Di Taman Nasional Bukit Barisan Selatan
Abstrak: Studi bambu di Taman Nasional Bukit Barisan Selatan bertujuan untuk mengetahui keanekaragaman bambu yang tersebar di Taman Nasional Bukit Barisan Selatan dan mengetahui daerah sebaran dan ekologinya. Penelitian ini didasarkan pada pemeriksaan spesimen koleksi bambu dari Taman Nasional Bukit Barisan Selatan. Spesimen tersebut diperiksa morfologi bagian vegetatif dan generatifnya. Dari spesimen-spesimen tersebut hanya tiga jenis yang mempunyai bunga, sedangkan yang lainnya tidak terdapat bunga. Pengamatan ciri morfologi dilakukan dengan melihat bagian-bagian buluh, tunas, pelepah buluh, daun dan perbungaan. Hasil pengamatan morfologi menunjukkan bahwa ada lima marga dengan 15 jenis bambu yang tumbuh tersebar di kawasan Taman Nasional Bukit Barisan Selatan. Marga - marga tersebut yaitu Bambusa, Dendrocalamus, Dinochloa, Gigantochloa dan Schizostachyum. Dari 15 jenis tersebut, 12 jenis telah dipertelakan oleh beberapa ahli sebelumnya yaitu: Bambusa vulgaris, Dendrocalamus asper, Dendrocalamus buar, Dinochloa glabrescens, Gigantochloa atroviolaceae, Gigantochloa apus, Gigantochloa atter, Gigantochloa hasskarliana, Gigantochloa robusta, Schizostachyum brachycladum, Schizostachyum zollingeri dan Schizostachyum bamban. Tiga jenis merupakan jenis baru dan telah dipertelakan yaitu Gigantochloa ciliata Rumyati, Gigantochloa denticulata Rumyati dan Schizostachyum bracteum Rumyati.....dst
Keyword: Gigantochloa ciliata, Gigantochloa denticulata, Schizostachyum bracteum

Judul: Ekologi dan Bioprospeksi Bambu di Kawasan Taman Wisata Alam Gunung Baung Pasuruan Jawa Timur
Abstrak: Bamboo is one of plant taxa comprising various species with high economic potential. Bamboo is a member of Bambusoideae of the Poaceae family. There are 1250 bamboo species in the world, 135 of them are in Indonesia. Not all bamboo species are well known, and only some of them can be found in Java Island. Land use changes reduce bamboo natural habitats in Indonesia. One of the bamboo forests in East Java is found at a conservation area namely Mount Baung Natural Park. The information of bamboo diversity, population structure and the relationship between bamboo and its biotic and abiotic factors in Mount Baung Natural Park is not well known. The aims of the research were to study the ecology of bamboo, the relationship between bamboo growth and its biotic and abiotic factors, and the bio-prospection aspect of this taxon in Mount Baung Natural Park, Pasuruan, East Java. This research was carried out at four different locations from September 2011 to Mei 2012. Those locations were Preserved Area, Rehabilitation Area, Limited Usage Area, and Intensive Usage Area. Data collection was conducted by analyzing the bamboo populations using sampled plots along the transects that were determined by stratified random samplings within four purposive sampled areas. The collected botanical data consisted of the bamboo scientific names, the number of clumps per plot and the diameter of clumps. The environmental data consisted of altitude, solar radiation, temperature-humidity, and soil pH. Soil samples were analyzed at the Soil Chemical Laboratory, Center for Research of Land Resources, Bogor. The abundances and densities of bamboo expressed an importance value index, namely the resultant of the sum of Relative Density, Relative Frequency, and Relative Dominance. The determination of habitat characteristics (i.e. determining the most related factors in relation to the presence of bamboo in their community) were analyzed by statistical correlations using the software STATISTICA Minitab 14. The principal component analysis (PCA) and cluster analysis were performed to determine relationships between abiotic/biotic components and bamboo’s occurrences. Canonical Correspondence Analysis (CCA) was used to determine component relationships. Cluster analysis was used to analyze the similarity of the environmental conditions of the bamboo habitat. Index Cultural Significance (ICS) was performed to determine the bamboo bio-prospecting of bamboo. There were seven species of bamboo in Mount Baung Natural Park, Pasuruan, namely Bambusa blumeana, Bambusa vulgaris, Dendrocalamus asper, Schizostachyum iraten, Gigantochloa apus, Gigantochloa atter and Dinochloa matmat. D. matmat was not described detail in this These due to the very limited number ( < 1%) of individual occurence. Among the six bamboo species, B. blumeana possessed nearly ideal population structure. Bambusa blumeana had the highest important value index 127,97%. The structure of bamboo population was classified based on the clump diameter classes which were: A (< 5 m), B (5-10 m) and C (> 10 m). Based on this category, the bamboo in Mount Baung Natural Park belonged to A phase class. Based on the soil analysis, the location had good soil characteristics and was feasible for bamboo growth, namely silty clay loam, medium CEC (Cation Exchange Capacity) ranging from 10,90 to 18,52
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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