Judul: Landscape Planning of Ecotourism in Malasari Village, Mount Halimun Salak National Park
Abstrak: Indonesia memiliki 53 taman nasional yang tersebar di seluruh penjuru negeri. Taman nasional dengan tingkat biodiversitas yang tinggi di Pulau Jawa yaitu Taman Nasional Gunung Halimun Salak (TNGHS). Upaya pelestarian TNGHS dihadapkan pada beberapa masalah. Salah satu masalah yang sering terjadi adalah adanya konflik kepentingan antara masyarakat dengan pengelola taman nasional dikarenakan pelanggaran pemanfaatan lahan dan sumber daya. Konflik ini terjadi di beberapa desa, salah satunya adalah Desa Wisata Malasari yang memiliki potensi tinggi untuk dikembangkan sebagai kawasan ekowisata. Namun, kegiatan ekowisata belum memberikan keuntungan yang nyata bagi masyarakat lokal sehingga performa ekowisata Desa Malasari perlu ditingkatkan dengan perencanaan lanskap yang mengintegrasikan potensi desa yang disesuaikan dengan karakteristik lanskap desa dalam mempertahankan fungsinya sebagai wilayah konservasi taman nasional dan hutan lindung. Tujuan dari penelitian ini adalah menyusun perencanaan lanskap kawasan ekowisata Desa Malasari, Taman Nasional Gunung Halimun Salak. Perencanaan lanskap dilakukan dengan pendekatan bioregion dan Ecotourism Opportunity Spectrum (ECOS). Metode perencanaan lanskap didasari pada analisis karakteristik lanskap bioregion, potensi objek dan daya tarik ekowisata, spektrum peluang ekowisata, serta preferensi dan akseptibilitas masyarakat. Hasil penelitian berupa perencanaan lanskap ekowisata yang didasari lima prinsip ekowisata dengan menguntamakan kelestarian lingkungan serta memberi manfaat ekonomi bagi masyarakat. Perencanaan lanskap kawasan ekowisata Desa Malasari, Taman Nasional Gunung Halimun Salak menghasilkan rencana ruang, rencana aktivitas dan fasilitas, rencana sirkulasi dan ekowisata, serta rencana vegetasi., Indonesia has a total of 53 national park around the country. One with the greatest biodiversity in Java is Mount Halimun Salak National Park. Throughout the conservation programs, the national park has faced with some problems, such as conflict of interest between communities and the park manager due to some villagers that has been utilizing park’s territory and resources. One of the village with such conflict is Malasari Tourism Village which has high potential to be developed into ecotourism district. However, these ecotourism activities have not provided tangible benefit for local communities so the ecotourism performance needs improvement by landscape planning integrating potentials with landscape characteristics of the village to maintain its function as a national park and protected forest. The purpose of this research is to arrange a landscape planning for the ecotourism area of Malasari Village, Mount Halimun Salak National Park. Landscape planning is carried out using the bioregion approach and Ecotourism Opportunity Spectrum (ECOS) approach. The landscape planning method is based on the analysis of bioregion landscape characteristics, ecotourism objects and attractions, the spectrum of ecotourism opportunities, also the preferences and acceptability of the inhabitants and visitors. The research result is ecotourism landscape planning based on five ecotourism principles prioritizing in environmental sustainability and providing economic benefits to the community. The landscape planning of ecotourism in Malasari Village, Mount Halimun Salak National Park produces spatial plans, activity and facility plans, circulation and ecotourism plans, and vegetation plans.
Keyword: bioregion, Ecotourism Opportunity Spectrum, Ecotourism, Landscape Planning, Malasari, Mount Halimun Salak National Park, ECOS, TNGHS

Judul: Ecotourism Landscape Planning for Loji Eagle Sanctuary in the Mount Halimun Salak National Park
Abstrak: Suaka Elang Loji (SEL) merupakan komplek wisata alam yang berada di zona pemanfaatan Taman Nasional Gunung Halimun Salak. Dikenal dengan kekayaan ekosistem dan ragam daya tarik wisata, khususnya Pusat Suaka Satwa Elang Jawa, SEL memiliki potensi pengembangan ekowisata yang bernilai tinggi dan unik. Namun, belum adanya penataan zona khusus rehabilitasi elang, potensi bencana lanskap, serta kurangnya fasilitas dan program wisata dinilai menghambat pengembangan ekowisata yang optimal. Oleh karena itu, penelitian ini bertujuan merencanakan lanskap untuk pengembangan ekowisata SEL yang berkelanjutan. Metode perencanaan lanskap menerapkan analisis spasial dan deksriptif pada aspek fisik-biofisik, sosial-ekonomi, wisata, dan legal dengan menggunakan pendekatan Regional Ecotourism Development Planning. Rencana lanskap menghasilkan konsep ekowisata yang memiliki nilai edukatif dan rekreatif bagi pengunjung. Untuk memenuhi fungsi rehabilitasi, konservasi, dan wisata, lanskap dikembangkan menjadi lima ruang utama yaitu ruang suaka, ruang konservasi, ruang penyangga dan rekreasi terbatas, ruang rekreasi, serta ruang penunjang wisata. Perencanaan ini meliputi rencana ruang, aksesibilitas dan sirkulasi, aktivitas dan fasilitas, vegetasi, serta daya dukung tapak., Loji Eagle Sanctuary is an ecotourism sites within the utilization zone of Mount Halimun Salak National Park. Renowned for its rich ecosystem and various tourist attractions, especially Javan Hawk Eagle Sanctuary Center, Loji Eagle Sanctuary holds high-value and distinctive ecotourism potential. However, the absence of limited zones for eagle rehabilitation, potential of landscape disasters, and the lack of facilities and tourism programs encounter the optimum landscape development. Therefore, this research aims to formulate a landscape plan for sustainable ecotourism development in Loji Eagle Sanctuary. Landscape planning methods apply spatial and descriptive analysis to physical-biophysical, socio-economic, tourism, and legal aspects using Regional Ecotourism Development Planning approach. The landscape plan produces an ecotourism concept with educational and recreational value for visitors. To fulfill the functions of rehabilitation, conservation, and tourism, the landscape is developed into five main zones, specifically the sanctuary zone, conservation zone, buffer and limited recreation zone, recreation zone, and tourism development zone. This plan includes a spatial plan, accessibility and circulation plan, activities and facilities plan, vegetation plan, also site carrying capacity plan.
Keyword: ekowisata, perencanaan lanskap, Suaka Elang Loji, Taman Nasional Gunung Halimun Salak

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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