Judul: Analisis Faktor- Faktor yang Memengaruhi Frekuensi Permintaan Konsumen terhadap Muslim Friendly Hotel di Kabupaten Belitung
Abstrak: Sejak tahun 2015 Kabupaten Belitung termasuk 10 daerah yang mempunyai destinasi wisata prioritas di Indonesia. Namun, jumlah hotel ramah Muslim masih sangat sedikit jumlahnya dan permintaan akan jasanya tidak terlalu tinggi. Untuk melihat kepuasan konsumen akan jasa layanan Muslim friendly hotel di Kabupaten Belitung, penelitian ini mencoba menganalisis dari konsumen yang berkunjung kembali di hotel yang sama. Tujuan penelitian ini adalah untuk menganalisis karakteristik dan faktor- faktor yang memengaruhi frekuensi permintaan konsumen terhadap Muslim friendly hotel di Kabupaten Belitung. Metode yang digunakan adalah analisis deskriptif dan analisis regresi linear berganda. Hasil penelitian ini menunjukkan bahwa karakteristik responden didominasi oleh perempuan, sudah menikah, rentang usia 26 hingga 35 tahun, pendidikan terakhir sarjana, pekerjaan sebagai pegawai swasta, lama bekerja kurang dari 5 tahun, pendapatan per bulan pada rentang Rp 3.000.000 – Rp.5.000.000 dan berdomisili Belitung. Faktor pendapatan, religiusitas, kualitas pelayanan, dan fasilitas berpengaruh positif dan signifikan terhadap frekuensi permintaan konsumen dalam menggunakan jasa layanan hotel ramah Muslim., Since 2015 Belitung Regency has been included in 10 regions that have priority tourist destinations in Indonesia. However, the number of Muslim-friendly hotels is still low and the demand for their services is not too high. To see consumer satisfaction with the service of Muslim friendly hotels in Belitung Regency, this study analyze the consumers who return to the same hotel. The purpose of this study analyze the factors that influence the frequency of consumer demand for Muslim Friendly Hotels in Belitung Regency. The method used are descriptive analysis and multiple linear regression analysis. The results showed that the characteristics of the respondents were dominated by women, married, age range from 26 to 35 years, the last education was undergraduate, work as a private employee, working period fewer than 5 years, monthly income in the range of Rp. 3,000,000 - Rp. 5,000. 000 and domiciled in Belitung. Factors of income, religiosity, service quality, and facilities have a positive and significant effect on the frequency of consumer demand in using Muslim-friendly hotel services.
Keyword: Frequency of demand, Muslim friendly hotel, Quality of service, Religiosity

Judul: Minat Masyarakat terhadap Wisata Ramah Muslim di Provinsi Banten
Abstrak: Salah satu bentuk pariwisata yang menjadi tren global adalah pariwisata ramah muslim. Banten memiliki potensi masuk ke dalam 10 besar pariwisata ramah muslim di Indonesia. Namun, hingga tahun 2021 wisatawan yang mengujungi Banten mayoritas berasal dari masyarakat Banten itu sendiri yaitu sebesar 77,10%. Penelitian ini bertujuan menganalisis minat masyarakat terhadap wisata ramah muslim di Provinsi Banten dengan mengambil sampel sebanyak 100 responden. Metode penarikan sampel yang digunakan adalah purposive sampling dan dianalisis menggunakan Structural Equation Model-Partial Least Square (SEM-PLS). Hasil penelitian menunjukkan bahwa faktor yang signifikan memengaruhi minat berkunjung masyarakat terhadap wisata ramah muslim di Provinsi Banten adalah sikap, norma subjektif, persepsi kendali perilaku, fasilitas, dan pengetahuan halal. Sementara, religiusitas tidak memiliki pengaruh yang signifikan terhadap minat pariwisata ramah muslim., One form of tourism that is becoming a global trend is muslim-friendly tourism. Banten has the potential to be among the top 10 muslim-friendly tourism in Indonesia. However, until 2021, the majority of tourists visiting Banten come from the Banten community itself, which is 77,10%. This study aims to analyze the Public interest in muslim-friendly tourism in the province of Banten by taking a sample of 100 respondents. The sampling method used was purposive sampling and analyzed using Structural Equation Model-Partial Least Square (SEM-PLS). The results showed that the factors that significantly influenced the public's interest in visiting muslim-friendly tourism in Banten Province were attitudes, subjective norms, perceptions of behavioral control, facilities, and knowledge of halal. Meanwhile, religiosity do not have a significant influence on the interest in muslim-friendly tourism.
Keyword: banten province, muslim friendly, tourism, visiting interest

Judul: Gene Editing  of Two Cyanide Synthesis-related Genes in Cassava using CRISPR/Cas9
Abstrak: Cassava (Manihot esculenta) contain toxic quantities of cyanogenic glucoside. Reducing the potentially toxic cyanogenic glucoside levels in cassava could be achieved by gene editing through Clustered Regularly Interspaced Short Palindromic Repeats – CRISPR associated protein 9 (CRISPR/Cas9), a high- precision genome editing technique in plants. Two cassava UDP- glucosyltransferase (UGT) paralogs, UGT85K4 and UGT85K5, are the genes catalyzing the synthesis of cyanogenic glucosides in plants. The study aimed to construct the CRISPR/Cas9 recombinant vector carrying single guide RNA (sgRNA) to edit UGT85K4 and UGT85K5 genes, and transform sgRNA CRISPR/Cas9 construct into KU50 cassava calli. Five sgRNAs were designed, considering the on/off target efficiency score and GC content. Those sgRNAs were ligated into pRGEB32 plasmid and introduced to competent Escherichia coli DH5a. The PCR colony and DNA sequencing were performed to verify the sgRNA insert. The amplicons showed the expected band size of recombinant vector (180 bp). Based on sequencing results, only one sgRNA was successfully ligated into the plasmid that will be used to edit the UGT85K5 gene. The sgRNA CRISPR/Cas9 construct in pRGEB32 plasmid was successfully transformed into Agrobacterium tumefaciens and introduced into KU50 cassava calli through co-cultivation. The overgrowth of Agrobacterium occurred after the co-cultivation phase.
Keyword: CRISPR/Cas9, Cyanogenic glucoside, Gene editing, sgRNA