Judul: Karakterisasi ekstrak kasar lakase dari isolat jamur liginolitik basidiomesetes Bsd
Abstrak: Lignin merupakan penyusun biomasa tumbuhan yang sulit untuk didegradasi. Salah satu cara untuk mengatasi hal tersebut adalah menggunakan enzim lakase yang dihasilkan oleh jamur Basidimisetes. Penelitian ini bertujuan untuk mengetahui aktivitas dan kondisi optimum reaksi enzim lakase dari isolat jamur Basidiomisetes Bsd menggunakan metode spektrofotomettri.
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Judul: Isolasi, Pemurnian, dan Karakterisasi Lakase dari Trametes versicolor KALT GK.
Abstrak: Lakase (EC 1.10.3.2) adalah salah satu enzim ekstraseluler yang banyak dimanfaatkan dalam proses degradasi lignin. Salah satu mikroba penghasil lakase adalah jamur pelapuk putih Trametes versicolor. Tujuan dari penelitian ini adalah mengisolasi, memurnikan, dan mengkarakterisasi lakase dari isolat lokal T. versicolor KALT GK yang ditumbuhkan pada media cair yang mengandung sekam padi. Lakase dimurnikan melalui pengendapan amonium sulfat pada tingkat kejenuhan 0-80%. Parameter yang dikarakterisasi adalah pH, suhu, stabilitas lakase terhadap variasi suhu, pengaruh ion logam, serta Km dan Vmaks. Lakase mengendap secara optimal pada amonium sulfat kejenuhan 0-20% dengan aktivitas spesifik sebesar 21.35 U/mg, kemurnian 1.86 kali dan perolehan 66.28%. Lakase hasil pemurnian mencapai kondisi optimum pada pH 6.0, suhu 60 oC, serta stabil pada suhu 30-40 oC. Penambahan ion logam Mg2+, Ca2+, Cu2+, Mn2+, dan Zn2+ dengan konsentrasi 1 mM berperan sebagai aktivator, sedangkan Fe2+ dan EDTA dengan konsentrasi 1 mM berperan sebagai inhibitor. Nilai Km dan Vmaks hasil pemurnian lakase adalah 1.00 mM dan 294.12 nmol/mL min.
Keyword: karakterisasi, lakase, pemurnian, sekam padi, Trametes versicolor KALT GK

Judul: Penilaian Risiko Kualitatif Pemasukan Virus Avian Influenza (H5N1) ke Pasar Burung Pramuka Jakarta Melalui Unggas
Abstrak: Traditional market or poultry/live birds market was identified as one of avian influenza (AI) virus spot circulation and very potential to contribute on virus spreading to human. Virus infection at the market could not be defined, but the risk value for virus infection can be estimated due to understand well its likelihood and risk factors need to be managed. Qualitative risk assessment had been known as a way to estimate its risk value. Qualitative risk assessment was conducted at Pramuka Birds Market with the purpose to identify biological risk pathways of the entering of Avian Influenza virus (H5N1) and to estimate its risk level through birds. Risk assessment had been done based on the qualitative risk assessment method published by Organization of Internationale Epizootica (OIE) on 2009. The research was conducted on February-June 2012 with primary and secondary data support. Primary data were collected through field observation and questionnaire for 30 sellers and 30 consumers respondents, and through indepth interview with 17 expert respondents, three middlemans and three intstitutions who concern with bird trading. The result of this study identified that there were six pathways of birds supplies to the market defined as risk biological pathways, which are entering of (1) domestic waterfowl, (2) chickens (kampoong, ornamental, or hobbies/pet chickens, (3) wetland birds, (4) “bridges species” birds, (5) songs birds, and (6) exotic birds. Critical control points had been identified in each birds supplies chain are birds collecting fascilities in catcher or collector level, kiosks or sale fascilities, farms, and entry/exit point. Source of birds for the market were (1) nature (capturing from nature), (2) farm, and (3) other country (through importation process). Generally, all groups of birds were important for bringing risk of AI virus entering to PB Pramuka, but both domestic waterfowl and chickens had identified as very import group to bring the risk. Risk estimation value of entering of AI virus (H5N1) to PB Pramuka Jakarta through six groups of poultry/birds originally come from nature, farm and other country was moderate, with the moderate uncertainty. Moderate risk estimation means that before some commodities to be approved for entering the area, it was mandatory to do assessment on risk management implementation, included on its visibility, effectivity, and mechanism of its verification. Based on risk management point of view, moderate risk estimation needed an efforts for risk management implementation to achieve the appropiate level of protection (ALOP) at “negligible” level. This ALOP’s level determined based on characteristic of AI which were zoonotic and had big risk included death both for animal and human. With AI endemic status for Indonesia, still known that AI virus potentially active for mutation, and the findings of high number on entering or selling birds at the market, implementation of applicable risk management must be applied on the birds supplies chain, from the origin until entry the market. Continous surveillance program, development and implementation of operational standard procedures and diseases early warning system, providing appropiate biosecurity fascilities at the market, and better implementation of biosecurity practices with law enforcement support are recommended as risk management actions that
Keyword: assessment, birds, Pramuka Bird Market, qualitative, risk