Judul: Evaluation of Influenza Antibody Titers in Serum and Egg Yolk of Layers Vaccinated with Influenza Vaccines.
Abstrak: Influenza belongs to the Orthomyxoviridae family which causes upper and lower respiratory tract infection. The aim of this study was to evaluate influenza-A specific antibody titers from serum and egg yolk of hens vaccinated with quadrivalent influenza vaccines. Three Hy-line Brown layers were vaccinated with quadrivalent influenza type A and B inactivated vaccines. Vaccination was carried out three times at a dose of 0.25 ml/chicken via the intramuscular route. The first vaccination was carried out at 24 weeks of age, then boosters were performed at 28 and 32 weeks of age. Serum and egg yolk were collected every week for 20 weeks since the first vaccination. Influenza A H3N2 and H1N1 antibody titers were determined using hemagglutination-inhibition (HI) test and agar-gel-precipitation (AGP) test. Influenza-A H3N2 and H1N1 specific antibodies were detected in both serum and egg yolk from the first to twenty weeks after the initial vaccination. Highest HI geometric mean titer (GMT) of H1N1 antibody reached 26.67 HIU at nine weeks post first vaccination (p.f.v), while H3N2 antibody reached 26 HIU at six weeks p.f.v. Our result showed that influenza antibodies can be produced in layers using quadrivalent vaccines and layer chickens are able to produce equivalent antibody levels for each influenza subtype.
Keyword: antibody, egg yolk, influenza, layer chickens, serum

Judul: Antibody Responses against Newcastle Disease and Evaluation of Infectious Bronchitis Maternal Derived Antibody on Vaccinated Layers.
Abstrak: Information of maternal derived antibody (MDA) and onset of immunity after vaccination is important to determine appropriate vaccination programme of chickens. The aim of this study was to evaluate duration of Infectious Bronchitis (IB) MDA and evaluate onset of immunity Newcastle Disease (ND) vaccination among layers. All layers were vaccinated using live ND and Infectious Bursal Disease (IBD) at one day old age, and received Avian Rhinotracheitis (AR) vaccine at 4 day old age. Serum samples were collected at various ages: 7, 10, 14, 20, 22, 24, 29 and 35 day old. The presence of ND antibodies was measured by the hemagglutination inhibition (HI) test, whilst the presence of IB antibodies was measured by enzyme-linked immunosorbent assay (ELISA). Our study showed that ND antibodies were at positive level until 24 day old age, and reached protective level ≥ 25 only at 7 day old age, whilst IB MDA were positive up to 17 day old age. This result indicating the needs of ND booster vaccination at least at 24 day old and IB vaccination at 17 day old age.
Keyword: antibody, Infectious Bronchitis, layers, Newcastle Disease, vaccination

Judul: Perilaku Petambak Dalam konservasi Hutan Mangrove di Desa Jayamukti, Kabupaten Subang, Provinsi Jawa Barat
Abstrak: Hutan mangrove memiliki fungsi penting, baik secara ekonomis maupun ekologis. Adanya kecenderungan peningkatan kegiatan mengkonversi kawasan mangrove untuk kegiatan non konservasi, dirasakan akan mengancam keberadaannya. Pengoperasian tambak berhubungan dengan berbagai faktor, diantaranya adalah aspek individu petambak dan ekosistem mangrove. Untuk itu perlu dikaji keragaan dan perilaku petambak dalam konservasi mangrove, serta hubungan antara kedua hal tersebut.
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