Judul: Sikap Pemirsa Terhadap Motto Liputan 6 Petang SCTV : Aktual, Tajam, Terpercaya
Abstrak: Secara umum tujuan penelitian ini adalah (1) mengetahui sikap individu mas_yarakat mengenai tayangan berita dengan motto aktual, tajam dan terpercaya Liputan 6 Petang SCTV, (2) Mengetahui hubungan karakteristik individu dengan opini pemirsa tentang motto aktual, tajam dan terpercaya, (3) Mengetahui hubungan perilaku komunikasi, desain siaran terhadap opini pemirsa tentang motto aktual, tajam dan terpercaya dan (4) Mengetahui hubungan sikap dan reaksi dengan opini pemirsa tentang motto aktual, tajam dan terpercaya. Pengambilan data meliputi pemirsa yang berdomisili di Jakarta, Bogor, Tangerang, dan Bekasi (Provinsi Jawa Barat). Waktu kegiatan bulan Februari sampai April 2006. Pengambilan sampel dilakukan secara purposif (purposive sampling), jenis data yang dikumpulkan meliputi data primer dan sekunder, pengumpulan data dilakukan dengan cara wawancara, observasi lapang, studi literatur, dan menghimpun data Research & Development SCTV. Alat analisis dengan menggunakan · analisis deskriptif dan korelasi Spearman. Hasil penelitian menunj.ukkan sikap responden terhadap berita-berita yang ditayangkan dalam Liputan 6 Petang SCTV masih relatif disenangi oleh masyarakat. berita-berita yang disajikan Liputan 6 Petang SCTV yang masih dibungkus dengan motto klasiknya, Aktual, Tajam, dan Terpercaya. Secara umum pemirsa Liputan 6 Petang SCTV memiliki kesamaan karakteristik yakni responden berumur rata-rata 21 - 40 tahun, pendidikan rata-rata adalah strata satu (S1) tahun, pekerjaan rata­rata sebagai pegawai dan swasta serta pendapatan rata-rata Rp. 600.000 - Rp. 1.000.000. Hasil analisis menunjukkan bahwa pemirsa di Jakarta Terdapat hubungan yang signifikan antara sikap dan reaksi dengan opini pemirsa terhadap motto "aktual", wilayah Bogor hanya antara reaksi dengan motto "tajam", wilayah Bekasi hubungan sikap dan reaksi tidak signifikan dengan motto dan wilayah Tangerang terdapat hubungan yang signifikan hanya antara sikap dengan motto "tajam". Dengan demikian hubungan opini pemirsa dari empat kota memiliki sikap dan persepsi yang berbeda-beda terhadap motto Liputan 6 SCTV.
Keyword: Observasi lapang, Komunikasi massa, Televisi, Purposive sampling, Wawancara

Judul: Motivasi dan Perilaku Menonton serta Penilaian Khalayak Terhadap Program Acara Televisi Lokal (Kasus Pemirsa Megaswara TV di RW 01 Kelurahan Bojong Rangkas, Kecamatan Ciampea, Kabupaten Bogor dan RW 17 Kelurahan Tegal Gundil Kecamatan Bogor Utara, Kota Bogor)
Abstrak: Megaswara TV has known well as the only local television in Bogor. The purpose of this research is to describe the motivation for people to watch Megaswara TV programs and the factors that influence it, finding corelation between motivation and people behaviour in watching the Megaswara TV programs, to describe the people behaviour in watching the Megaswara TV programs and the factors that influence it, and to describe judgement from people who watch the program in Megaswara TV. This research held in two places, one held in Bojong Rangkas village RW 01, Ciampea subdistrict and the other one held in Tegal Gundi village RW 17,Nort Bogor subdistrict, West Java Province. This research using quantitative methode and support by qualitative data. This research give us some results that shown some correlations between several factor which affect people to watch Megaswara TV, these correlation are between level of education with the information motivation, between etnic with personal identity motivation, between family influence with integration and social interaction motivation, between integration and social interaction motivation with the frequency and how long can people watch the show, between entertainment motivation with integration and social interaction motivation, between personal identity motivation with the frequency and how long can people watch the show, between information motivation with how long can people watch the show, between age with watching frequency to fill personal identity needs, between education level with show option and watching duration to fill personal identity needs, between etnic with show option to fill personal identity needs, between age with watching frequency to fill integration and social interaction needs, between education level with show option to fill entertainment needs, between age with watching frequency to fill entertainment needs, between hometown with integration and soial interaction motivation, between hometown with watching frequency to fill entertaiment needs. It is more likely to say that most correspondend have a good judgement for the Megaswara TV shows, not just for the show it self, but the local content, the presenter, the quality of the broadcast, the broadcast time, the benefits, and the actual effects get good credits from most correspondend. Keywords: local television, tv watching behaviour, tv watching motivation
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Judul: Gene Editing  of Two Cyanide Synthesis-related Genes in Cassava using CRISPR/Cas9
Abstrak: Cassava (Manihot esculenta) contain toxic quantities of cyanogenic glucoside. Reducing the potentially toxic cyanogenic glucoside levels in cassava could be achieved by gene editing through Clustered Regularly Interspaced Short Palindromic Repeats – CRISPR associated protein 9 (CRISPR/Cas9), a high- precision genome editing technique in plants. Two cassava UDP- glucosyltransferase (UGT) paralogs, UGT85K4 and UGT85K5, are the genes catalyzing the synthesis of cyanogenic glucosides in plants. The study aimed to construct the CRISPR/Cas9 recombinant vector carrying single guide RNA (sgRNA) to edit UGT85K4 and UGT85K5 genes, and transform sgRNA CRISPR/Cas9 construct into KU50 cassava calli. Five sgRNAs were designed, considering the on/off target efficiency score and GC content. Those sgRNAs were ligated into pRGEB32 plasmid and introduced to competent Escherichia coli DH5a. The PCR colony and DNA sequencing were performed to verify the sgRNA insert. The amplicons showed the expected band size of recombinant vector (180 bp). Based on sequencing results, only one sgRNA was successfully ligated into the plasmid that will be used to edit the UGT85K5 gene. The sgRNA CRISPR/Cas9 construct in pRGEB32 plasmid was successfully transformed into Agrobacterium tumefaciens and introduced into KU50 cassava calli through co-cultivation. The overgrowth of Agrobacterium occurred after the co-cultivation phase.
Keyword: CRISPR/Cas9, Cyanogenic glucoside, Gene editing, sgRNA