Judul: Isolation and Characterization of Acyl Homoserine Lactonase (AHL-lactonase) Producing Bacteria from Agricultural Field in Java
Abstrak: Quorum sensing (QS) is a bacterial communication mechanism correlating with the number of bacterial population and accumulation of autoinducer (AI). Quorum sensing in Gram-negative bacteria involves a AI group of acyl homoserine lactone (AHL) to regulate the expression of several genes, including virulence genes. Anti-quorum sensing compounds can degrade AHL so that virulence genes can not be expressed. Acyl homoserine lactonase (AHL-lactonase) encoded by the aiiA gene is one enzyme that can act as an anti-quorum sensing. This study aimed to isolate and characterize the AHL-lactonase producing bacteria isolated from agricultural field in Java. Activity of AHL degradation bioassay using Chromobacterium violaceum as a bacterial indicator. Verification the presence of AHL-laktonase was performed by gene aiiA amplification. As many as 12 bacterial isolates showed AHL degradation activity and two isolates had the aiiA gene i.e. INT1c and SGT3g isolates. These isolates had bacil cells with chain arrangement and endospores. They were grouped in Gram-positive bacteria. AiiA gene sequence analysis showed that aiiA gene of INT1c had homology with aiiA gene of Bacillus cereus ATTCC 14579 while the SGT3g isolate had homology with aiiA gene of Bacillus thuringiensis BMB171. Molecular identification based on 16S rRNA gene showed that INT1c isolate had the closest similarity with the Bacillus cereus strain GM3 (98%) while SGT3g isolate had the closest similarity with the Bacillus thuringiensis strains A1-1 (99%).
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Judul: Isolasi dan karakterisasi bakteri penghasil ahl-laktonase asal lahan pertanian luar Pulau Jawa
Abstrak: Quorum sensing (QS) is communication between bacterial cells mediated by autoinducer (AI) to regulate the expression of specific genes in certain bacterial population quorum. AI of Gram negative bacteria is N-acyl homoserine lactone (AHL). QS system controls behaviors of bacterial population on biofilm formation, virulence, bioluminescence, and formation of antibiotics. QS process can be inhibited by anti-QS compound. AHL-lactonase is one of anti-QS compound encoded by aiiA gene. The aims of this study were to isolate and characterize bacteria producing AHL-lactonase from agricultural land outside Java. Soil samples were taken from 16 locations of agricultural lands. Bioassay was performed by AHL degradation of Chromobacterium violaceum. Detection of aiiA gene was conducted using specific primer aiiAF and aiiAR, while molecular identification was conducted based on 16S rRNA gene sequence. It was found that from 58 isolates, six isolates had AHL degradation activity i.e. PAD1a, BAL2a, NTT3a, NTT3e, NTT6B6, dan NTT6B7 isolates. Two isolates (i.e. NTT3a and NTT3e isolates) had aiiA gene. And indicated by 800 bp product. They were 99% closely related to AHL-lactonase gene from Bacillus sp 91.
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Judul: Perhitungan Kebutuhan Tendik PNS Berdasarkan Analisis Beban Kerja Pada Biro Umum dan Keuangan Universitas Negeri Padang
Abstrak: Diketahui dalam rentang tahun 2022 hingga 2025 akan ada 6 orang tendik PNS yang pensiun pada BUK UNP, namun BUK UNP belum pernah melakukan perhitungan Analisis Beban Kerja (ABK) sebelumnya. ABK sendiri merupakan salah satu persyaratan dalam pengusulan formasi CPNS bagi instansi sehingga perhitungan ABK akan sangat berpengaruh terhadap jumlah formasi CPNS yang masuk untuk tiap instansi. Tujuan penelitian ini yaitu, (1) menganalisis jumlah beban kerja tendik PNS pada BUK UNP dan (2) menganalisis kebutuhan tendik PNS berdasarkan analisis beban kerja pada BUK UNP. Metode yang digunakan dalam penelitian ini yaitu metode analisis beban kerja menurut Permenpan RB Nomor 1 Tahun 2020 dan FTE. Metode nonprobability sampling dengan teknik purposive sampling dipakai sebagai penarikan sampel. Hasil perhitungan menunjukkan bahwa dibutuhkannya penambahan 8 orang serta pemindahan 3 orang pegawai ke unit lain pada Biro Umum dan Keuangan UNP dengan total pegawai optimal 5 orang.
Keyword: Analisis Beban Kerja, Analisis Jabatan, Analisis Kebutuhan Pegawai, Full Time Equivalent (FTE)