Judul: Change of hydrocarbon Compounds During Bioremediation Processes of Soils Polluted by Crude Oil Using Gas Chromatography Mass Spectrophotometry.
Abstrak: Pollution handling that caused by crude oil contamination can use crude oil degrading microorganism. In this reseach change of hydrocarbon compounds degraded by bacteria were investigated with gas chromatography mass spectrophotometry (GCMS). The soils mixed with 5 % (b/b) crude oil was added with T2M bacteria isolated. The change of hydrocarbon compounds and total petroleum hydrocarbon (TPH) were measured every two weeks. TPH mesurement worked by gravimetry method with n-hexane solvent. Preparation for GCMS worked by soxhlet extraction. Extraction finished for 4 hours with 300 ml aseton:n-hexane (1:1) solvent. The extract dried by rotaryevaporator and measured with GCMS. The result of this reseach show that the biggest TPH decrease happened in nonsterilized soils and added bacteria with finally TPH 0.28 % and 93.3 % degradation. Soils were not added by bacteria had finally TPH 0.37 % and 90.17 % degradation. The soils were sterilized and added by bacteria had finally TPH 0.37 % and 90.17 % degradation, even the soils were sterilized and were not added by bacteria had finally TPH 1.37 % and 74.54 % degradation. The change hydrocarbon compounds during degradation process happened to long chain hydrocarbon became shorter hydrocarbon chain. This is showed by the decrease of percent area on chromathograme. The soils were not sterilized and added by bacteria showed the biggest changing because long chain hydrocarbon at the finally measurement were not detected.
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Judul: Perombakan Hidrokarbon Dalam Tanah Terkontaminasi Minyak Berat, Minyak Ringan dan Oli Bekas oleh Bacillus sp.
Abstrak: Oil is the main source of energy for humans activities such as industries and transportations. The oil exploration activities have potential to pollute the environment (i.e.soil, water, and air). The sludge released bysuch activities can be degraded by bacteria into non-harmful compounds. Biodegradation process is done to achieve the concentration of Total Petroleum Hydrocarbon (TPH) in the contaminated soil less than 1%. The result showed that the bacteria of Bacillus sp. ICBB 7859, ICBB 9461, ICBB 5071 were able to degrade the concentration of TPH of heavy oil from 9.5082% to 0.4673%, 10.7747% to 0.3607% and 9.2442% to 0.6713%, respectively. The three isolates of bacteria needed nine weeks to complete the process. For light oil, Bacillus sp. ICBB 7859 was able to decrease the TPH from 2.9407% to 0.4378%, ICBB 9461 from 3.1433% to 0.4702%, and ICBB 5071 from 3.0742% to 0.5991% respectively, within four weeks. For iv lubricant wastes, Bacillus sp. ICBB 7859 was able to decrease the TPH of lubricant wastes from 7.6949% to 0.9067, ICBB 9461 from 7.4556% to 0.9222%, and ICBB 5071 from 7.3459% to 0.6796% respectively. The three isolates of bacteria completed the degradation process in different time. Bacillus sp. ICBB 7859 and ICBB 9461 degraded lubricant wastes within six weeks, and Bacillus sp. ICBB 5071 degraded the lubricant wastes within five weeks to reach the TPH concentration of less than 1%.
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Judul: Karakteristik Anatomi Skelet Kepala Buaya senyulong (Tomistoma schlegelii).
Abstrak: Buaya sepit atau senyulong (Tomistoma schlegelii) merupakan jenis buaya asli Indonesia yang berhabitat di sungai-sungai Sumatera, Kalimantan, dan juga pedalaman Sulawesi. Salah satu ciri khas buaya senyulong adalah bentuk moncong yang runcing dan sempit, namun hingga saat ini informasi mengenai struktur skelet kepala buaya senyulong sangat terbatas. Penelitian ini bertujuan untuk mempelajari karakteristik anatomi skelet kepala buaya senyulong yang dikatikan dengan fungsi dan perilakunya. Penelitian dilakukan secara eksploratif dengan melakukan pengamatan, pencatatan, dan pengambilan gambar dari skelet kepala buaya senyulong. Skelet kepala berukuran panjang 70 cm, lebar 29 cm, dan tingggi 17 cm dengan permukaan kasar pada os nasale, os frontale, os maxilla, os mandibula, os lacrimale, os jugale. Jumlah gigi pada buaya ini sebanyak 78 buah, terdiri dari 4 buah gigi premaxila, 16 buah gigi maxila, dan 19 buah gigi mandibula. Pada regio orbitalis ditemukan dua pasang fenestrae temporales yang berkembang baik. Fenestra supratemporale merupakan lubang oval yang membentang di sepanjang os parietale. Fenestra infratemporale berbentuk segitiga dan dibatasi oleh os squamosum, os postorbitale, os jugulare, dan os quadratum. Os mandibula hewan ini mempunyai symphysis mandibularis yang panjang yaitu garis yang melintang di corpus mandibulae. Secara fungsional ukuran symphysis mandibularis berkaitan dengan adaptasi hewan terhadap diet makan.
Keyword: kepala, moncong, Tomistoma schlegelii