Judul: Persistensi vaksin DNA penyandi glikoprotein 25 yang diberikan melalui pakan buatan pada ikan mas (Cyprinus carpio)
Abstrak: Koi Herpes Virus (KHV) is a major viral pathogen that infects common carp and koi. KHV disease outbreak in almost all centre of common carp culture in Indonesia and caused mass mortality. DNA vaccination method is one of ways to cope with KHV infection. Vaccines were commonly given by injection. In this research, persistence test of DNA vaccine (pMBa-GP25) were carried out by oral feeding on common carp. DNA vaccine persistence test were done using PCR method with the specific primer for GP25 gene. Escherichia coli DH5α bacteria containing pMBa-GP25 DNA vaccine were cultured in four treatment mediums namely 2xYT, LB polypeptone, LB triptone and TSB. Bacteria densities were counted with spectrophotometer using 580 nm wave lengths. The results showed that an efficient culture media for E. coli was LB tripton. Furthermore, GP25 DNA was detected in kidney and spleen of vaccinated fish. Level of DNA vaccine that could be detected was 7,56 ng (amount of DNA copy 1,598 x 1010 copies).
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Judul: Efektivitas Frekuensi Pemberian Vaksin DNA melalui Pakan terhadap Kelangsungan Hidup Relatif Ikan Mas yang Diinfeksi Koi Herpesvirus
Abstrak: Cultured of common carp is currently constrained by a koi herpesvirus (KHV) disease. KHV disease is highly malignant, infectious, and can kill common carp and koi over 80% of the population. It is therefore necessary to provide a prevention method that are safe and can be mass applied. In this study, it carried out anti-KHV DNA vaccination 1 mL/fish through artificial feed to determine the effective frequency of vaccination that produces a high survival on KHV infected fish. There are five treatments with three replicates, namely treatment A: vaccination once a week, treatment B: two times a week of vaccination, treatment C: vaccination three times a week, positive control: the fish were not vaccinated and challenged with KHV, and negative controls: fish were not vaccinated and injected with phosphate buffer saline solution. Feeding was performed 2 times a day at satiation. The results showed that vaccination three times a week offered 4 the highest relative percent survival (84.6%) and showed the best phagocytic activity compared with other treatments. Hence, vaccination via the feed can effectively increase the survival of fish, and this can be as an alternative method to control KHV infection in carp and koi culture.
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Judul: Pemanfaatan Buluh Bambu Tali Sebagai Komponen Pada Konstruksi Rangka Batang Ruang
Abstrak: Salah satu bentuk konstruksi yang banyak digunakan sebagai konstruksi rangka atap adalah konstruksi rangka batang (truss) yang kemudian berkembang menjadi konstruksi rangka batang ruang (space truss). Dengan kelurusan bambu yang terbatas, buluh bambu sesuai untuk digunakan pada konstruksi rangka batang ruang, karena konstruksi ini tersusun dari komponen-komponen yang relatif pendek. Pada konstruksi ini, komponen-komponen batang dihubungkan secara sendi, sehingga beban yang bekerja pada batang hanya gaya aksial tekan dan tarik. Bambu diketahui mempunyai kuat tarik yang tinggi dengan kuat geser serta kuat belah yang rendah, sehingga dari sudut mekanika bahan cocok untuk dimanfaatkan pada konstruksi rangka batang. Bentuk bambu yang berupa tabung agak tirus (tappered) dengan diameter yang beragam, selama ini dianggap sebagai hambatan dalam pemanfaatannya di bidang konstruksi. Selain itu adanya buku dengan jarak yang tidak seragam menjadi kendala dalam membuat sambungan, khususnya sambungan yang dapat dianalisa secara mekanika.
Keyword: buckling properties, tensile strength, resistance force, inner part of bamboo, West Java