Judul: Analisis Ekologi Bentanglahan untuk Penentuan Potensi Sumberdaya Air (Studi Kasus: DAS Cimadur, Banten)
Abstrak: Indonesia was predicted would have water crisis in 2025 in the World Water Forum II in the Hague in March 2000. Most of the cause has been by the weaknesses in water management. Study of water and its high demand has been associated with shapes and physical characteristics of an area. Topographic factors play an important role in determining spatial pattern water resources. In this research, Topographic Wetness Index (TWI) is implemented to determine spatial pattern of surface water saturation zone. Cimadur watershed was selected as one of main watersheds in Lebak, Banten which has been contributing on flood hazard. Study on TWI in Cimadur watershed has became important because it could show distribution points containing concentration of water and therefore useful for determining potential inundation as well as areas potentially storing water in the watershed. This research aims to: (1) Identify types of landform, land use, and slopes using remote sensing data (Google Earth imagery, ALOS AVNIR-2 imagery, and SRTM imagery), (2) Conduct analysis of TWI to obtain the distribution of spatial pattern of surface water saturation zones, and (3) Conduct an analysis of the landscape ecology (landform, land use, slope, and grade TWI) using landform as unit of analysis for determination of potential water storage. Landscape ecological analysis shows that Cimadur watershed is dominated by Tertiary denudational volcanic landforms (DV1 and DV2), which covers 10.046 Ha, mixed-use garden covers 8.952 Ha, the slope of 15-30% (steep) covers 8.534 Ha, and middle TWI class (= grade 2) covers 20.987 Ha. It shows that ecological conditions in test site are still at safe water saving, however, the area is fairly vulnerable to climate change or land cover/land use change. These are due to dynamics of water flow in the area during rainy and dry seasons.
Keyword: 

Judul: Conservation Strategy for Ciliman Dam Catchment Area, Banten
Abstrak: Daerah tangkapan air (DTA) Bendung Ciliman Banten merupakan sumber air bagi Bendung Ciliman, sehingga perlu dilakukan konservasi agar keandalan air bendung dapat terjamin. Perubahan tataguna lahan akibat pertumbuhan penduduk di DTA telah menyebabkan menurunnya luas hutan dan meningkatnya kekritisan lahan. Tujuan penelitian ini adalah (1) mengidentifikasi kondisi ekologi, sosial, dan ekonomi DTA Bendung Ciliman; (2) mengetahui status keberlanjutan DTA Bendung Ciliman; (3) menyusun strategi konservasi DTA Bendung Ciliman yang berkelanjutan. Metode yang digunakan antara lain analisis deskriptif, analisis klasifikasi tutupan dan penggunaan lahan, analisis koefisien rezim aliran (KRA), analisis Multi Dimensional Scaling (MDS), dan analisis SWOT. Hasil analisis klasifikasi tutupan dan penggunaan lahan menunjukan bahwa penggunaan lahan di DTA Bendung Ciliman di dominasi oleh perkebunan 49,87% dan semak belukar 28,99%. Area pemukiman dan gedung relatif kecil hanya sebesar 1,76%. Sedangkan untuk luas hutan masih kurang yaitu hanya sebesar 3,41%. Hasil analisis KRA didapatkan dari data debit maksimum Bendung Ciliman selama 1 tahun sebesar 33,6 m3/detik dan debit minimum sebesar 0,49 m3/detik. Sehingga dihasilkan hasil KRA sebesar 68,41. Hasil analisis keberlanjutan daerah tangkapan air Bendung Ciliman Banten menunjukkan hasil cukup berkelanjutan dengan nilai 52,52%, dengan nilai stress 12,98% dan nilai R2 sebesar 95,45%. Berdasarkan hasil analisis leverage menunjukkan nilai atribut yang paling sensitif terhadap keberlanjutan daerah tangkapan air terdapat 7 atribut pengungkit, antara lain pada dimensi ekologi terdapat 3 atribut yaitu penggunaan lahan, persentase lahan kritis, dan luas hutan. Pada dimensi ekonomi terdapat 2 atribut yaitu jumlah koperasi dan nilai tukar petani (NTP), sedangkan pada dimensi sosial terdapat 2 atribut yaitu tingkat pengangguran dan tingkat layanan pendidikan. Hasil analisis SWOT berdasarkan hasil analisis matriks evaluasi komponen internal dan komponen eksternal, didapatkan hasil akhir analisis internal factor analysis summary (IFAS) sebesar 0,74 dan external factor analysis summary (EFAS) 0,29. Berdasarkan hasil analisis SWOT, maka strategi yang perlu diterapkan antara lain memanfaatkan laju pertumbuhan penduduk yang rendah untuk meningkatkan laju pertumbuhan ekonomi di DTA Bendung Ciliman, tutupan vegetasi di DTA Bendung Ciliman cukup tinggi dapat dimanfaatkan oleh masyarakat sekitar hutan untuk membentuk koperasi yang dapat meningkatkan nilai ekonomi hutan., The catchment area (DTA) of the Ciliman Dam in Banten is a source of water for the Ciliman Dam, so it needs to be conserved so that the reliability of Dam can be guaranteed. Changes in land use due to population growth in catchments have led to a decrease in forest area and increased land criticality. The objectives of this study are (1) to identify the ecological, social and economic conditions of the Ciliman Dam catchment area; (2) knowing the status of sustainability in the Ciliman Dam catchment area; (3) develop sustainable Ciliman Dam catchment area with conservation strategies. The methods used include descriptive analysis, classification analysis of land cover and land use, flow regime coefficient analysis (KRA), multi dimensional scaling (MDS) analysis, and SWOT analysis. The results of classification analysis of land cover and land use show that land use in the Ciliman Dam catchmen area is dominated by plantations of 49.87% and shrubs of 28.99%. Residential areas and small-scale buildings are only 1.76%. Meanwhile, the forest area was still insufficient, namely only 3.41%. The
Keyword: catchment area, Ciliman Dam, sustainability status, SWOT

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
Keyword: