Judul: Development of selection method corn parent inbred lines for parents at hybrid waxy corn tolerant to drought and introgression of opaque-2 (oo) gene by using SSRS (Simple Sequence Repeats)
Abstrak: The Dissertation study the development of selection method corn parent inbred lines for parents at hybrid waxy corn tolerant to drought and introgression of opaque- 2 (oo) gene by using SSRs (Simple Sequence Repeats). The first experiment was to determine relation of waxy corn genotype kinship based on DNA band pattern by using SSRs marker and correlation among taxonomic distance matrix averages based on morphological performance, and similarity coefficient based on DNA band pattern getting from SSRs marker. The result showed that genetic variance of the tested material was high enough. It was indicated by polymorphism value of 0.62. Dendogram construction based on UPGMA was able to distinguish one genotype to the others. According to genetic similarity, the genotypes could be grouped into three clusters characterized by the genotypes with similar pedigree code with cofenetic correlation coefficient (r) 0.88 categorized good fit for handled waxy corn genotype groups. However, Correlation value between taxonomic distance matrix based on morphological performance and similarity coefficient based on DNA band pattern according to goodness of fit criteria was categorized as very weak. The information could used in selecting the parent and become as one of strategies in hybrid maize breeding program. The second experiment was conducted to estimate combining ability and heterosis value of ten waxy corn genotypes having high amelopectin, tolerance to drought stress environment as parent candidate of hybrid formation. The result indicated that several genotypes having good combining ability value for grain weight character per plant and interval silking anthesis under stress and drought stress environment. Grain weight character per plant on without stress and drought stress environment showed the same combining ability value, while interval silking anthesis on without stress and drought stress environment revealed different combining ability value. This information is important to determine direct or indirect selection method in selecting maize genotypes which tolerant to drought stress environment. The third experiment was conducted to introgress recessive gene opaque-2 mutant from QPM genotypes to waxy corn genotypes by using MAS (Marker Assisted Selection). The result showed that specific SSRs marker phi057 was proved effective and efficient to be used in selecting genotypes of CML141 x PTBC4-7-1- BB and CML142 x PTBC4-10-1-BB progenies that were introgressed with homozygote recessive opaque-2 (oo) gene. Selection result utilizing MAS had a tendency to follow relative segregation on every selfing generation and the number of CML141 x PTBC4-7-1-BB and CML142 x PTBC4-10-1-BB progenies were screen with phi057 specific marker. Was identified 57 genotypes of PTBC4-7-1- BBo2 and PTBC4-10-1-BBo2 as genetic materials in development of hybrid maize variety having high amilopectin and protein content. The fourth experiment was carried out to obtain information on yield potency and waxy corn genotypes having opaque-2 gene as parent candidate of hybrid variety development. The result showed that the tested genotypes had good specific combining ability value good enough to one of the good testers either with CML144 or CML156 for grain weight character per plant, and grain weight character performance of top crossing 57 genotypes with the two very varied testers. The effect of environment factor was more dominant than
Keyword: Seleksi Galur Murni, Jagung Pulut, waxy corn, Simple Sequence Repeats

Judul: Genetic distance analysis based on ssrs and morphological marker and combining ability analysis for sweet corn development (Zea mays L. Var. Saccharata) hybrid.
Abstrak: The objective of the research were to grouping sweet corn lines resistant to downy mildew, high specific and general combining ability and high heterosis to select parental line to breed single cross hybrid of sweet corn. The first research was done to study genetic variability of 39 sweet corn lines based on SSR and morphological marker to select good parental lines of hybrid sweet corn. There were high genetic variability among genetic material, showed by genetic distance value about 0.18 to 0.78, indicates wide genetic variability of sweet corn. The results shows that among 20 lines having genetic similarity between 0.25 – 0.82 or genetic distance from 0.18 to 0.75; based on morphological trait 0.27 – 1.00. Correlation value between similarity matrix based on SSR and morphology was high on 0.88 of goodness of fit criteria. The aim of second research was to study general combining ability of sweet corn lines for yield and resistant to downy mildew for hybrid varieties improvement. Six of 45 lines having high fresh ear yield per plot and best general combining ability, such as Mr4/SC/BC4-2-1B, Mr4/SC/BC4-6-1B, Mr11/SC/BC4-2-1B, Mr11/SC/BC4-4-1B, Mr12/SC/BC4-6-1B, and Mr12/SC/BC3-3-1B. All of testing lines have resistance to downy mildew with severity about 0.0 to 7.1%. The lines having high GCA value would be good combiner to other lines to develop high yielding hybrid sweet corn and resistant to downy mildew. The third research was done to study general and specific combining ability of sweet corn lines and higher heterosis of downy mildew resistance supporting the hybrid corn development. The combination of A x D lines (Mr12/SC/BC4-6-1B x Mr11/SC/BC4-2-1B) were medium genetic distance and having highest GCA, heterosis and heterobeltiosis for fresh ear yield per plot character. The D x C combination (Mr11/SC/BC4-2-1B x Mr4/SC/BC4-2-1B) have wide genetic distance and high fresh ear yield per plot, heterosis and heterobeltiosis, but have negative SCA value. The combination of E x C (Mr12/SC/BC3-3-1B x Mr4/SC/BC4-2-1B) have a wide genetic distance but low productivity. The best hybrid is not always obtained from parents with the high genetic distance, but also can be obtained from the low or medium genetic distance as well.
Keyword: Hybrid sweet corn, Zea mays L. var. saccharata, Genetic variability

Judul: Infeksi Virus Parainfluenza Pada Anjing
Abstrak: Virus parainfluenza anjing termasuk famili Paramyxoviridae, berbentuk pleomorf dengan ukuran bervariasi (bergaris tengah antara 100-500 nm), berpembungkus peka ether, berisi genom RNA dan secara serologis mempunyai kesamaan dengan virus SV-5 kera; dikenak sebagai salah satu kausa penting dari sindrom "kennel cough". Infeksinya dapat mengenai anjing semua umur yang belum pernah terinfeksi atau mendapatkan vaksinasi mengakibatkan gejala klinis ringan yang terbatas pada traktus respirasi atu infeksi yang secara klinis tidak memperlihatkan gejala nyata. Gejala klinis yang nampak setelah masa inkubasi (1-5 hari) terlewati berupa batuk yang tidak berat selama beberapa hari, berbagai tingkat tonsilitis dan pharyngitis dan kadang-kadang ditemukan kenaikan temperatur rektal antara 1-2 derajat C dari temperatur normal rat-rata. Anjing terinfeksi mengandung virus pada traktus respirasi bagian atas dan bawah serta limfonoduli sampai hari ke 9 setelah infeksi. Penyebaran virus antar anjing terjadi secara aerogen.
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