Judul: Analisis Transkriptomik Pohon Sengon (Falcataria moluccana Miq.) Rentan dan Resisten terhadap Hama Boktor (Xystrocera festiva Pascoe).
Abstrak: Sengon (Falcataria moluccana Miq.) plantations in monoculture system has increases boktor (Xystocera festiva Pascoe) pest population and distribution, however there is no effective and efficient control method yet. This study aims to analyze sengon transcriptomic data and identify expressed genes involved in sengon resistance to boktor pests, also other genes related to abiotic and biotic stresses. Unpublishe de novo transcriptomic data was acquired using the RNAseq and deposited in the DDBJ repository with accession number DRA008389. DEG analysis was perfomed using R program with edgeR package. The results of gene screening were then annotated using genes analog with the Swissprot database. DEG analysis produced 6859 upregulated contigs, genes in resistant tree were Heat shock cognate 70 kDA protein, Trypsin inhibitor, and Chalcone synthase. Based on previous research, trypsin inhibitor is a defensive mechanism of sengon against boktor pests, while other genes are known to be involved in the abiotic and biotic stress response.
Keyword: de novo transcriptomic, Falcataria moluccana Miq, trypsin inhibitor, Xystocera festiva Pascoe

Judul: α-Amylase Inhibitor Gene Expression of Healthy Sengon (Falcataria moluccana Miq) Healthy and Attacked by Boktor Pests
Abstrak: Sengon (Falcataria moluccana Miq) merupakan salah satu pohon pionir dan memiliki berbagai macam kegunaan. Penanaman sengon secara monokultur menimbulkan terjadinya ledakan hama penggerek batang atau boktor (Xystrocera festiva) yang merusak kayu dan menjatuhkan nilai jual batang sengon. Studi molekuler aktivitas gen α-amilase inhibitor (AAI), yang merupakan salah satu gen ketahanan terhadap hama boktor sangat mendukung program pemuliaan sengon. Penelitian ini bertujuan mengkuantifikasi tingkat ekspresi gen α-amilase inhibitor sengon yang sehat dan terserang hama boktor. Prosedur penelitian ini meliputi isolasi RNA total, sintesis cDNA, perancangan primer spesifik gen AAI (target) dan aktin (kontrol), optimasi suhu annealing primer gen AAI dan aktin, ekspresi gen AAI dan aktin pada Real Time PCR dan analisis data. Fragmen cDNA teramplifikasi pada suhu annealing 61,7oC untuk gen aktin dan 61oC untuk gen AAI. Tingkat ekspresi gen AAI 2 pada pohon sengon yang terserang hama boktor memiliki nilai yang lebih tinggi yaitu 55,60 pada sampel 1 dan 6,96 pada sampel 3.
Keyword: Falcataria moluccana, α-Amilase Inhibitor, Real Time PCR

Judul: Metrik Einstein (Anti-) Self-Dual Berdimensi Empat dengan Metode Cartan
Abstrak: Gravitational field equation can be formulated in differential forms (differential geometry), which describes gravity as a manifestation of the curvature of space-time. Curvature of space-time is characterized by a metric that satisfies the gravitational field equation. With differential forms it can be formulated the (anti-) self-dual Einstein equation, which is a modification of the Cartan structure in (anti-) self-dual basis. Calculation of (anti-) self-dual metric with (anti-) self-dual Einstein equation is simpler than the calculation using Christoffel symbols.
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