Judul: Isolation and Cloning of P5CS (pyrrolin-5-carboxylate synthetase) Gene from Rubber Plant
Abstrak: Rubber plant (Hevea brasiliensis) is one of the plantations that has important role in Indonesia. Molecular technique by using P5CS gene as molecular marker is selection method that faster and more accurate for producing rubber plant that compatible for marginal land. The aim of this research is to isolate and clone P5CS gene as a marker gene of drought resistant. Steps of this research are isolation of rubber plant DNA genome, isolation of P5CS gene from rubber plant by P5CS CS forward-P5CS CS reverse and P5CS start-P5CS stop primers, cloning into pGEM-T easy plasmid, isolation of plasmid DNA, sequencing, and analyzing DNA sequence. Isolation of rubber plant DNA genome is successfully proceed. Isolation of P5CS gene by using P5CS CS forward-P5CS CS reverse primer is not been successfully proceed, while isolation gene by using P5CS start-P5CS stop primer produce amplicon that has 2250 bp length. The confirmation of P5CS gene cloning by PCR technique using M13 forward-M13 reverse primer produce DNA that has 2450 bp length. The length of plasmid DNA that has been isolated is 5450 bp. The result of DNA sequence analysis by BLASTX shows that amplicon which has been isolated is not P5CS gene, but it has high homology (68%) with drought resistant gene.
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Judul: Seleksi Primer Simple Sequence Repeat (SSR) untuk Identifikasi 17 Klon Karet (Hevea brasiliensis)
Abstrak: Rubber tree (Hevea brasiliensis) breeding have problem because it takes long time and wide area to select new superior clones. On the other hand, the effort to increase rubber trees planting is not followed by availability of original clone seeds. Quick and accurate identification is needed to solve these problems. One of strategy that can be used to solve these problems is by using Simple Sequence Repeats (SSR) molecular marker. The aim of this research was to select SSR primer that identify the 17 rubber clones specifically by its DNA pattern and polymorphism degree. Nine primers used to amplified the 17 DNA of rubber clones. SSR results was evaluated by 3% agarose and 6% polyacrilamide. Eight of nine primers in this research can identify some rubber clones. Those primers are SSRH403, SSRH548, gSSR165, gSSR212, gSSR268, EHB109, EHB169, and HBE280. The most informative primer that is SSRH403 (PIC: 0.7069) can identify five clones, while the weakest primer that is HBE280 (PIC: 0) can identify just one clone.
Keyword: Bogor Agricultural University (IPB), PIC, SSR, Hevea brasiliensis, rubber tree

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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