Judul: Metode Review Scoring untuk menilai kualitas ulasan barang pada aplikasi jual beli online Bukalapak.
Abstrak: Review adalah salah satu fitur pada aplikasi jual beli online yang memungkinkan pengguna untuk membaca dan memberikan umpan balik tentang kualitas produk. Review yang tidak berfokus pada produk dapat menyulitkan calon pembeli untuk mengetahui kualitas produk yang sesungguhnya...
Keyword: Bukalapak, jual beli online, Regresi logistik, review scoring, ulasan

Judul: Metode Alternatif dalam Pencarian Peringkat E-commerce di Indonesia Berdasarkan Penilaian Pelanggan.
Abstrak: Keberadaan internet memunculkan sistem perdagangan secara online menggunakan aplikasi. Maraknya sistem perdagangan secara online memicu muculnya berbagai e-commerce di Indonesia yang menyediakan berbagai macam kebutuhan pelanggan. Hal ini juga menimbulkan permasalahan bagi pelanggan yaitu kesulitan dalam memilih e-commerce yang berkualitas. Upaya yang dilakukan untuk mengatasi masalah ini adalah membuat peringkat e-commerce di Indonesia berdasarkan dari penilaian pelanggan. Metode yang umum digunakan untuk pemeringkatan adalah metode Analytical Hierarchy Process (AHP) tetapi dalam praktiknya terdapat beberapa peubah yang tidak terdapat di e-commerce sehingga metode AHP tidak bisa digunakan. Metode alternatif yang dipilih adalah metode optimasi Ant Colony Optimation (ACO). Pengujian kelayakan metode ACO dalam pencarian peringkat untuk data e-commerce perlu dilakukan karena tidak semua peubah terdapat di e-commerce. Simulasi untuk pencarian peringkat dilakukan menggunakan 2 skenario data bangkitan dengan metode AHP dan ACO. Hasil simulasi didapatkan kesimpulkan metode ACO layak digunakan untuk pemeringkatan yang terdapat data kosong sampai 15%, selanjutnya dilakukan penerapan metode ACO untuk data e-commerce di Indonesia dengan kondisi data kosong 6.25%. Hasil tabu terbaik berdasarkan nilai peluang tertinggi, feromon terendah, dan koefisien korelasi tertinggi yaitu pada tabu ke-2, dengan tiga peringkat teratas yaitu JD, SP, dan TP.
Keyword: ant colony optimation, analytical hierarchy process, e-commerce, rating

Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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