Judul: Motivasi menonton film di bioskop dan pengaruhnya terhadap perubahan perilaku khalayak : Kasus pada bioskop di kota Bogor, propinsi Jawa Barat
Abstrak: Menonton film di bioskop pertama kali dilakukan di Perancis pada tanggal 28 Desember 1895. Pada saat itu film dianggap oleh khalayak sebagai sarana hiburan. Menonton film mengalami perkembangan sampai ke Indonesia, yaitu pada tanggal 5 Desember 1900 dengan adanya pertunjukkan film yang diputar di tanah lapang. Pada saat itu, film juga hanya dianggap sebagai sarana hiburan saja. Film sebagai salah satu media komunikasi massa, selain sebagai sarana hiburan juga sebagai sumber pengetahuan dan informasi dengan membawa pesan- pesan yang relatif mudah ditangkap oleh orang-orang sederhana sekalipun. Pesan- pesan tersebut mengandung makna. Makna pesan berbeda-beda tergantung pada interpretasi khalayak yang berbeda-beda pula. Pesan dalam film mengandung isi pesan sehingga khalayak dapat memahaminya. Isi pesan tersebut meliputi jenis dan cerita film. Pesan dalam film bioskop dapat memotivasi khalayak untuk tetap menonton film di bioskop tersebut. Motivasi pada khalayak dapat disentuh oleh motif-motif tertentu. Motif adalah alasan seseorang sehingga dapat memotivasi untuk melakukan sesuatu. Motivasi atau daya dorong khalayak untuk menonton film di bioskop didasarkan pada kepuasan dan kebutuhan kontak sosial. Motivasi ini meliputi motivasi intrinsik dan ekstrinsik. Motivasi intrinsik adalah motivasi yang berasal dari dalam diri khalayak (internal) dan ekstrinsik dari kekuatan luar (eksternal). Menonton film di bioskop, selain memiliki motivasi yang berbeda- beda pada setiap khalayak juga pengaruh yang berbeda pula. Berdasarkan hal tersebut, maka ingin diketahui jenis film, motivasi dan pengaruh menonton film di bioskop terhadap perubahan perilaku khalayak di Kota Bogor. Pada penelitian ini metode yang digunakan adalah sengaja (purposive)…dst
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Judul: Persepsi, pengetahuan, dan perilaku menonton film layar lebar pada siswa SMA Negeri 3 Kota Bogor
Abstrak: The rapid development of movie industries over the past years makes producers compete in making more movies. Facts shows that Indonesian movie produces only think of profits alone, without regarding to the benefits and impact of a movie. This study aims to analyze the perceptions, knowledge, and behaviors on SMA 3 Bogor high school students towards watching movies.Respondents are SMA 3 Bogor students who had watched a movie in the past year.The study involved 100 students were selected conveniently. Data is collected through interviews using questionnaires. The data obtained were analyzed using descriptive and inference statistics. Results showed that students had a perception less amenable towards the movies with the level of knowledge in the middle category. In the meantime, the watching behavior of students are in the low category. Pearson correlation test results shows that the amount of students pocket money alone have a significant relationship towards the behavior of watching a movie.
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Judul: Excretory/secretory antigen of ascaridia galli L3 stage to trigger yolk immunoglobulin in laying hens
Abstrak: Ascaridiosis caused by the nematode Ascaridia galli. A study was carried out to characterize excretory/secretory antigen of A. galli from larval stage to trigger anti-ascaridiosis yolk immunoglobulin in laying hens. A. galli L3 were recovered from intestines of 100 heads chickens 7 days after oesophagus inoculation with 6000 L2. L3 recovered in this manner were cultured (5 – 10 ml-1) in flasks containing rosswell park memorial institute (RPMI) 1640 media, pH 6.8, without phenol red. Cultures were incubated at 370C in 5% CO2 and culture fluid was collected after 3 days in culture. To prepare excretory/secretory antigen, culture medium were concentrated with vivaspin 30.000 MWCO, and antigen protein concentration were counted as described in Bradford method. The molecular weight of excretory/secretory antigen was determined with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). Active immunizations were applied intra muscularly of chickens with an initial dose of 80 μg. The immunizations were repeated three times with dose of each 60 μg with an interval of one week. The first immunizations were excretory/secretory antigen mixed with fruend adjuvant complete and subsequently mixed with freund adjuvant incomplete. The chicken’s eggs were collected from 49 day after immunizations. Anti-ascaridiosis yolk immunoglobulin (IgY) was extracted from egg yolks by means of ammonium sulphate and purified using fast performance liquid chromatography (FPLC). The purity of anti-ascaridiosis IgY was determined by Bradford method (λ = 280 nm). Antibody was detected by agar gel precipitation test (AGPT) and enzyme linked immunosorbant assay (ELISA). Antigen were detected with immunohistochemistry. Chickens were challanged with dose of 1000 L2 A. galli. Passive immunizations were applied ten times with dose of each 0,875 mg IgY with an interval of one day intra orally. IgY anti-ascaridiosis was tested against A. galli survival in vitro.
Keyword: Unggas, Isolasi dan karakterisasi antigen, Purifikasi imunoglobulin yolk, Pengukuran antibodi ayam petelur, Metode Imunohistokimia, Ascaridiosis