Judul: Preferensi Konsumen dan Keputusan Pembelian Konsumen Kedai Kopi di Kecamatan Kajen Kabupaten Pekalongan
Abstrak: Minum kopi sudah menjadi lifestyle masyarakat Indonesia. Bisnis kedai kopi merupakan salah satu usaha pemasaran agribisnis yang sedang berkembang cukup pesat di Kecamatan Kajen, Kabupaten Pekalongan. Pertumbuhan ini terlihat sejak 2019. Faktor-faktor yang membentuk preferensi konsumen di setiap daerah berbeda-beda. Penelitian ini bertujuan untuk menganalisis faktor pembentuk preferensi konsumen dan keputusan pembelian konsumen kedai kopi di Kecamatan Kajen. Metode pengambilan responden yang digunakan adalah accidental sampling dengan jumlah 150 responden. Metode analisis data yang digunakan adalah analisis deskriptif dan analisis faktor. Berdasarkan hasil penelitian, diketahui bahwa mayoritas konsumen yang berkunjung ke kedai kopi karena ingin berkumpul bersama teman, mendapatkan sumber informasi dari teman, menu kopi menjadi fokus perhatian ketika mengunjungi kedai kopi, frekuensi kunjungan 1-3 kali dalam sebulan, dan akan kembali mengunjungi kedai kopi. Faktor pembentuk preferensi konsumen terdiri dari delapan faktor yaitu harga, sarana pendukung, variasi dan cita rasamakanan dan minuman, pelayanan, fasilitas, variasi dan cita rasa minuman selain kopi, lokasi, dan diskon dan tampilan.
Keyword: coffee shop, consumer preferences, consumer purchasing decision

Judul: Analysis of Consumer Preferences in choosing a Coffee Shop in Bontang City
Abstrak: Kopi telah menjadi bagian dari gaya hidup dan digemari oleh masyarakat sehingga menjadi usaha yang menjanjikan. Semakin menjamurnya coffee shop di Kota Bontang menyebabkan persaingan bisnis sehingga terjadi fluktuasi penjualan. Untuk pemasaran yang efektif, pemilik coffee shop perlu memahami preferensi konsumen di Kota Bontang. Penelitian ini berfokus pada coffee shop di Kota Bontang Kalimantan Timur, dengan tujuan menentukan strategi berdasarkan perilaku konsumen. Metode penelitian menggunakan analisis konjoin dengan sampel 147 responden melalui purposive sampling. Lokasi menjadi atribut terpenting, diikuti oleh atmosfer, harga, fasilitas, pelayanan, dan produk. Konsumen cenderung memilih coffee shop yang dekat dengan tempat tinggal, harga Rp 10.000 – Rp 15.000, fasilitas Wifi, pelayanan cepat, dan tampilan menu yang menarik. Implikasi strategis dari penelitian ini dapat membantu pemilik coffee shop memahami preferensi konsumen dan meningkatkan pemasaran mereka di Kota Bontang., Coffee has become a part of lifestyle, embraced by the community and promising as a lucrative business. The proliferation of coffee shops in Kota Bontang has intensified business competition, leading to sales fluctuations. For effective marketing, coffee shop owners need to understand consumer preferences in Kota Bontang. This study focuses on coffee shops Bontang East Kalimantan, aiming to determine strategies based on consumer behavior. The research method employs conjoint analysis with a sample of 147 respondents selected through purposive sampling. Location emerges as the most crucial attribute, followed by atmosphere, price, facilities, service, and products. Consumers tend to choose coffee shops close to their residences, with prices ranging from Rp 10,000 to Rp 15,000, Wifi facilities, prompt service, and visually appealing menu displays. The strategic implications of this research can assist coffee shop owners in understanding consumer preferences and enhancing their marketing efforts in Kota Bontang.
Keyword: Consumer Preference, Coffee Shop, Conjoint Analysis

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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