Judul: Analisis Molekuler dan Fenotipe Galur Padi Transgenik IR64 Generasi T3 yang Mengandung Penanda Aktivasi untuk Toleransi Kekeringan
Abstrak: Activation tagging transgenic rice cv. IR64 had been developed by introducing a construct of activation tagging transposon Ac/Ds. Phenotipic character associated with drought stress tolerance of the transgenic rice lines have not been analyzed. This study aimed to evaluate T3 generation of activation tagging transgenic rice lines cv. IR64 to drought stress treatment. The methodology of study consisted of several steps including germination of samples seeds, screening the seedling with drought strees treatment, molecular analysis of the rice lines identify hpt and bar genes, and observation of agronomic character. Results of study showed that there were 34 out of 84 plants that tolerant to drought stress. A total of 23 plants of tolerant plants were detected carrying only bar gene after PCR analysis and indicated that the plants were stable mutants. Generaly, compared to the non-transgenic plants, there were changes in several agronomic parameters of transgenic rice plants cv. IR64 T3 generation. A total of nine plants increased of its height, six plants had a faster harvesting time, and 21 plants had higher weight of 100 seeds compared by non-transgenic plants.
Keyword: Bogor Agricultural University (IPB), drought stress tolerance, activation tagged, Rice (Oryza sativa L.)

Judul: Over-expression of OsDREB1A gene for improvement of drought tolerance on rice.
Abstrak: Drought is a major factor that influence the growth and productivity of rice. Drought tolerant rice varieties are needed to increase rice production. Development of improved rice varieties can be done through classical breeding and genetic transformation. The research objective was to obtain rice plants containing the construct 35S::OsDREB1A. Agrobacterium tumefaciens mediated transformation of rice cv. Nipponbare using OsDREB1A gene under the control of CAMV 35 promoter produced 81 putative transgenic rice plants (T0) positively containing the transgene based on PCR analysis. The plant height, panicle number, panicle length, and the grain number of transgenic plants were in general lower than the Nipponbare wild type. T1 seeds from selected genotypes were screened for hygromycine resistence, and rice seedlings were evaluated for tolerance to drought stress in the glasshouse, based on the standard evaluation system for rice (IRRI 1996). Five independent transgenic rice plants were identified to be tolerant to drought stress. Molecular characterization using Southern hybridization showed that these plants had 2- 4 copies of transgene. Physiological analysis showed that the drought avoidance is the possible mechanism of adaptation to drought of the transgenic plants. The drought tolerant transgenic plants were crossed to Indonesian elite rice cultivars (Ciherang, Batutegi, Code, and Konawe). Glasshouse evaluation showed that F1 transgenic plants derived from cultivars Batutegi, Code, and Konawe were more tolerant to drought than their wild-type counterparts. F1 transgenic plants were backcrossed into corresponding elit rice cultivars, and PCR analysis showed that those plants contained the transgene. These results showed that 35S::OsDREB1A could be transferred and inherited to elite rice genotipes through crossing. The new drought tolerant germplasms generated in this research can be useful materials for breeding program for improvement of drought tolerance on rice in Indonesia.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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