Judul: Identification of Rice Aromatic Properties Using Molecular Aromatic Gene-based Marker.
Abstrak: Rice as a basic commodity can be distinguished between aromatic and nonaromatic. Its different based on gene deletion at chromosom 8. Conventional methods like assessment was method which frequently uses to detect aromatic characteristic of rice. Molecular gene based marker was new method to identify aromatic rice accurately and shorthen analyzing time. The purpose of this research is to distinguish the type of aromatic and nonaromatic variety using aromatic gene-based marker. DNA as rice genetic material was isolated from leaves using CTAB method which broke the cell wall and release the components thus precipitated the DNA. DNA amplification conducted by PCR machine using specific aromatic primer and the result verified by 1.5 % gel agarose electrophoresis. The result of Bradbury primer obtained size of band about 580 bp and 355 bp for nonaromatic, also 580 bp and 257 bp for aromatic. Mentik Wangi and Gunung Perak variety could distinguish to band pattern. Furthermore, RM223 primer can be differentiate both of patterns of aromatic and nonaromatic rice by the band size about 120 bp to 160 bp.
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Judul: Analisis Sidik Jari DNA Padi Beras Merah, Padi Aromatik, dan Padi Genjah
Abstrak: Rice is one of major crops comodities with a high fenotype diversity. It can be divided into 3 cluster, red rice, aromatic rice, and genjah rice. The differences between those cluster, can be seen from fenotype and genotype of each variety using molecular markers based on gene. The objective of this research is to identify the specific character of red rice, aromatic rice, and genjah rice and the genetic diversity using Simple Sequence Repeat (SSR) molecular markers. Material genetic from rice was isolated using isolation DNA standard method, break the cell wall, expel parts of the cell, and precipitate the DNA parts (Sambrook method), and then the isolate was amplified using polymerase chain reaction (PCR) amplification procedure. Subsequently, analysis by association, evolution, and linkage (TASSEL) software was used for data processing. Genetic variation is based on the result of DNA amplification using PCR process from rice variety used. The results of this research showed the variation of DNA bands from 100 bp until 350 bp of red rice, aromatic rice, and genjah rice varies, also there is association between fenotype and genotype of variety in cluster. TASSEL was used to obtain description of variety and produced a dendrogram that show us the variety type of those variety, also which variety and markers has a significant result in amplification procedure.
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Judul: Fasciloliasis Pada Hewan Ternak Di Indonesia
Abstrak: Fascioliasis adalah salah satu penyakit hewan yang sangat merugikan yang disebabkan infeksi cacing hati (Fasciola sp.). Cacing hati termasuk kelas Trematoda dari famili Fasciolidae, bentuknya pipih seperti daun de- ngan ukuran bervariasi. Cacing ini termasuk hewan hermoprodit dimana alat kelamin jantan dan betina berada dalam satu tubuh. Di Indonesia hanya ditemukan satu spesies cacing hati yang menyerang hewan ternak yaitu Fasciola gigantica (Mukhlis, 1984 komunikasi pribadi). Siklus hidup cacing hati memerlukan siput sebagai induk semang antaranya. Di Indonesia siput ini ialah Lymnaea rubiginosa. Menurut Hall (1977) cacing hati yang sudah dewasa di dalam pembuluh empedu induk semang utama akan bertelur, tiap ekor cacing dapat menghasilkan 3000-3500 telur/hari. Fascioliasis dapat bersifat akut atau kronis tergan tung faktor umur, jumlah cacing yang menginfeksi dan jenis hewan ternak. Pada domba penyakit ini lebih sering bersifat akut, sedang pada sapi biasanya bersifat kronis. Pada keadaan akut biasanya hewan penderita tiba-tiba mati tanpa menimbulkan gejala klinis, bila ada gejalanya terlihat hanya sebentar. Gejala klinis pada bentuk akut yaitu lemah, nafsu makan berkurang, mudah lelah bila ber jalan dan sikapnya berbaring dengan bagian dada serta hidung kena ke tanah (Taylor, 1964). ...
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