Judul: Penerpan Analisis Struktur Jaringan Sosial Pada Jaringan Twitter Akun@matematikaipb.
Abstrak: Twitters is one of the popular social media in cyber space. Each of twitter user has different centrality weights based on its positions i n yhe network structure. The centrality calculations is compared in term of to the number of friendss of users.
Keyword: Twitter, Social Network Analysis, Centrality

Judul: Analisis Popularitas IPB berdasarkan Data Twitter Menggunakan Awan Kata, Geovisualisasi, dan Pemodelan Klasifikasi
Abstrak: Twitter merupakan media sosial yang sangat aktif digunakan di Indonesia. Twitter menjadi suatu media untuk para data analis untuk menggali informasi mengenai masalah-masalah yang ada di media sosial. Karakteristik yang dilihat adalah mengenai topik yang sering dibahas mengenai IPB, pemetaan frekuensi tweets pada berbagai wilayah di Indonesia, dan perbandingan tweets dengan 9 universitas lain terbaik di Indonesia. Hal yang akan dibandingkan adalah program pendidikan, sumber daya manusia, dan kemahasiswaan. Pelabelan respon tersebut akan menggunakan otomatisasi dengan memanfaatkan metode pemodelan klasifikasi seperti regresi logistik, support vector machine, mutlilayer perceptron, bayes naif, dan hutan acak. Penelitian ini akan menggunakan awan kata (word cloud) untuk mengetahui topik yang akan di bahas. Kemudian, tweets akan dipetakan secara geovisualisasi berdasarkan lokasi pada bio pengguna pada suatu tweets. Hasil dari penelitian ini menjelaskan bahwa IPB memiliki respon yang positif terhadap topik kemahasiswaan namun masih cenderung negatif pada beberapa topik yang lain. Manfaat dari penelitian ini diharapkan dapat menjadi bahan pertimbangan untuk Humas IPB dalam melakukan strategi sosialisasi IPB kedepannya.
Keyword: awan kata, geovisualisasi, IPB, klasifikasi, twitter

Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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