Judul: Uji potensi Artemia sp. sebagai vektor pembawa vaksin DNA untuk benih ikan mas Cyprinus carpio
Abstrak: Koi Herpes Virus (KHV) is one of the most common impetuses for disease on common carp Cyprinus carpio. Generally, viral disease is difficult to cure because virus is intra-cellular parasite, that virus survives, multiplies, and lives only if it on the host cell. The DNA vaccine that gift with oral method by Artemia sp. is one alternative that expected to handle fish viral disease. This experiment was carried out to analysis DNA vaccine expression encoding of glycoprotein gene (GP11) on Cyprinus carpio. Bacterial that has contents plasmid Krt-GP11 as vaccine is served through Artemia sp as vaccine carrier. Artemia sp containing the DNA vaccine was prepared for once or twice provisions a week to three week old common carp. The existence of DNA vaccine in intestine, kidney, and gill is analyzed by PCR method. The organs were analyzed every 3 days after vaccination. The expression of GP11 in kidney organs in each treatment is also observed by the use of RT-PCR method. Expressions in the organs were analyzed 10 days after vaccination. The experiment shows that dose of DNA vaccine in whole bacteria could be expressed is 106 cfu in a once or twice provisions a week. DNA vaccine could be detected in three organs. Also, RT-PCR analysis shows that the expression of GP11 can be detected in all tested organs. In conclusion, Artemia sp. can be employed as a vector to carry for plasmid GP11 vaccine with 90 minutes submersion.
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Judul: Efikasi Vaksin DNA Penyandi Glikoprotein Koi Herpesvirus pada Ikan Mas Stadia Benih melalui Perendaman
Abstrak: Koi herpesvirus (KHV) is a herpesvirus which especially infects and causes mass mortality to koi and common carp. Therefore the protection of carp from KHV infection is urgently needed. In this study, we developed an application of DNA vaccine containing gene encoding KHV glycoprotein by immersion method to increase survival of common carp against KHV infection. As many as 400 of 30-day-old common carp juveniles were immersed in the water containing 1.3 x 108 CFU/ml of the killed Escherichia coli cells carrying DNA vaccine. Treatments performed were variation of duration and frequency of DNA vaccine immersion; as follows: 1x 30 minutes, 1x 60 minutes, 1x 90 minutes, 2x 90 minutes, and 3x 90 minutes of immersion treatments. Reverse transcription polymerase chain reaction (RT-PCR) analysis showed that DNA vaccine was successfully expressed among the vaccinated fish. Twenty eight days post vaccination, the fish were challenged by injecting 10-4 ml/fish of KHV. The result demonstrated that vaccination by 30 minutes immersion; allowed the 61% of fish survived, this was significantly different (P<0.05) compared to control (without vaccination), but insignificantly different (P>0.05) compared to those of other treatments. The relative percent survival of vaccinated fish were also similar among different treatments (P>0.05). Vaccination by immersion have increased the survival rate about 2 fold compared to unvaccinated fish control (26.67%). Thus, DNA vaccine was effective delivered by immersion for 1x 30 minutes, and this technique can be useful to level up the resistance to KHV infection in common carp juveniles.
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Judul: Pembentukan tanah berbahan induk andesit-basaltik dan satuan-satuan tanah menurut sistem soil taxonomy pada suatu toposekuens di daerah Ujung Berung Bandung
Abstrak: Tujuan penelitian adalah untuk melihat satuan-satuan tanah pada toposekuens lereng G.Manglayang, Ujungberung, Bandung menurut sistem Soil Taxonomy dalam hubungannya dengan pembentukan dan perkembangan tanah yang didasarkan pada sifat fisik, kimia, dan kandungan mineralnya sebagai hasil dari proses penambahan, pengurangan, pemindahan, dan pengubahan dalam sistem di dalam profil tanah. Pada daerah perbukitan, faktor topografi sangat berperanan dalam proses pembentukan dan perkembangan tanah. Faktor topografi mempengaruhi pergerakan air baik secara vertikal maupun horisontal, sehingga berpengaruh terhadap tingkat kelembaban, proses pelapukan, pencucian dan pemiskinan serta proses erosi dan deposisi. Proses-proses ini akan menentukan sifat-sifat tanah secara fisik, kimia maupun kandungan mineralnya. Topografi semakin cembung menunjukkan tingkatan umur pembentukan tanah yang lebih lanjut dan cenderung membentuk tanah-tanah bersifat oksik dengan kandungan mineral liat tipe 1:1, yaitu Kaolinit, sehingga tingkat kesuburannya lebih rendah. Sedangkan pada topografi cekung cenderung terbentuk tanah muda, yaitu tanah belum berkembang dan mulai berkembang dengan kandungan mineral amorf. Pada topografi rektilinier cenderung terbentuk tanah berkembang dengan pembentukan horison Argilik.
Keyword: Sifat fisik, Sifat kimia