Judul: Variasi Gen Pengkode Subunit E1β Branched-Chain α- Keto Acid Dehydrogenase Complex Pada Kambing Kacang Dan Kosta
Abstrak: Kompleks branched-chain α-keto acid dehydrogenase (BCKDH) merupakan kompleks multi enzim yang memiliki peran penting dalam regulasi katabolisme asam amino bercabang (branched-chain amino acids, BCAA). Informasi mengenai gen BCKDH telah dilaporkan pada sapi, sedangkan pada ruminansia kecil seperti kambing dan domba hingga saat ini belum ada laporan. Tujuan dari penelitian ini adalah memetakan variasi ekson 1, 3, dan 4 dari gen pengkode subunit E1β branched-chain α-ketoacid dehydrogenase complex (BCKDHB) pada kambing kacang (n=2) dan kosta (n=2) jantan. Proses amplifikasi DNA dengan teknik PCR menggunakan pasangan primer AF515-AF516, AF519-AF520, dan AF521-AF522 adalah sekuen ekson 1 (64 bp), ekson 3 (134 pb), dan ekson 4 (156 pb) yang conserved pada keempat sekuen yang dianalisis. Situs yang bervariasi terdapat pada daerah 5’UTR (C10G dan A11C), intron 1 (G199C, C302T, C461T, C538T, dan C583T), dan intron 4 (T553C dan A758G). Penyejajaran hasil deduksi asam amino menunjukkan bahwa keempat sekuen kambing identik dengan sekuen asam amino dari kambing hitam Yunnan yang telah dipublikasikan di GenBank (XM_005684454 dan NC_022301). Hal ini menunjukkan bahwa variasi nukleotida yang terdapat pada intron 1 dan 4 merupakan mutasi netral.
Keyword: BCAA, BCKDHB, kambing kacang, kambing kosta

Judul: Keragaman Gen Branched Chain Ketoacid Dehydrogenase E1-α Polypeptide (BCKDHA) Ekson 2 dan 3 pada Sapi Madura
Abstrak: Branched Chain α-Ketoacid Dehydrogenase (BCKD) enzyme complex have a role in metabolism of Branched Chain Amino Acid (BCAA). BCKDHA gene encoding the first subunit of these enzyme complex within mithochondrion. Mutation in BCKDHA gene caused deficiency of BCKD complex that caused Maple Syrup Urine Disease (MSUD). The aim of this research is to detect variability of BCKDHA gene in madura cattle using Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) method. According to migration pattern of single strand in PAGE 9%, there was four types found in exon 2 and 3 BCKDHA gene in madura cattle, that is A, B, C and D. There was two types of gene found in cattle that its origin from Bangkalan, which is A and C, whereas type that found in Sampang was four, which is A, B, C and D. The highest frequency in two locations is type A, in Bangkalan (83%) and in Sampang (50%).
Keyword: BCKDHA, exon 2, exon 3, gene, madura cattle, SSCP, gene type.

Judul: Utilization of terra/MODIS L1B data for analysis of horizontal wind profile in the troposphere
Abstrak: This study was concerned with horizontal wind derived from data collected with the Moderate-resolution Imaging Spectroradiometer (MODIS) on board the NASA’s Earth Observation System (EOS)-Terra satellite. The aim of this study is to develop a method for the estimation of horizontal wind movement in the troposphere based on Navier-Stokes equation. The method was applied to a calibrated and geolocated Terra/MODIS L1B 1 km data set. The data inputs to estimate surface temperature are retrieved from TIR (Thermal Infrared) bands 31 (10.780 μm to 11.280 μm) and 32 (11.770 μm to 12.270 μm). The obtained surface temperatures were later used to approximate geopotential height on a constant-pressure surface by using the modified Poisson equation. The main assumptions used are the hydrostatic equilibrium, ideal gas law, constant lapse rate and constant acceleration of gravity. Based on the distribution of geopotential height, the pressure gradient force is measured by the gradient of geopotential on an isobaric coordinate to present the horizontal wind profile. For certain topographic areas especially in the mountain ranges, there was a disadvantage of using pressure as a vertical coordinate which lead to inaccuracy. In spite of that, Terra/MODIS L1B data with 1 km spatial resolution can be used to estimate the horizontal wind movement in the troposphere.
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