Judul: Perencanaan Lanskap Untuk Pelestarian Lanskap Kawasan Situs Percandian Batujaya Di Kabupaten Karawang
Abstrak: Kawasan Situs Percandian Batujaya berada di Kecamatan Batujaya, Kabupaten Karawang, Provinsi Jawa Barat. Kawasan ini merupakan kompleks percandian bercorak Buddhistik yang dibangun pada masa Kerajaan Tarumanegara. Peningkatan yang pesat terhadap pembangunan di Kabupaten Karawang mengancam tergesernya kawasan yang memiliki nilai bersejarah. Oleh karena itu, tujuan penelitian ini yaitu merencanakan lanskap pelestarian Situs Percandian Batujaya. Tahapan penelitian ini terdiri atas pengumpulan data, analisis, sintesis, penyusunan konsep, dan perencanaan. Analisis dilakukan untuk mengidentifikasi kesesuaian tata ruang candi dengan konsep kawasan candi berdasarkan ajaran agama Budha Mahayana sehingga dapat ditentukan unit ruang signifikansi sejarah kawasan. Hasil peta komposit unit ruang signifikansi ini digunakan sebagai arahan penyusunan konsep ruang dan aktivitas serta fasilitas pendukung. Tingkat signifikansi lanskap dipertimbangkan dalam penyusunan zonasi pelestarian rencana lanskap. Hasil akhir penelitian ini dituangkan dalam rencana lanskap.
Keyword: Kabupaten Karawang, perencanaan lanskap, Situs Batujaya, zonasi pelestarian

Judul: Landscape Planning of Historic City Center of Karawang West Java
Abstrak: Karawang adalah sebuah kabupaten di Provinsi Jawa Barat yang memiliki nilai historis yang tinggi, dengan akar sejarah sejak tahun 414. Pusat Karawang Kota mengalami perubahan dari berbagai periode sejarah yang membuat kawasan terus berkembang dan bertransformasi menjadi lanskap bersejarah. Terlepas dari nilai-nilai sejarah kawasan, saat ini kawasan dikenal sebagai pusat perdagangan dan penyebaran agama Islam. Seiring dengan perkembanganya, kawasan pusat Karawang Kota menerima tekanan dari lingkungan sekitarnya termasuk pembangunan perumahan dan banjir karena daerah ini dekat dengan Sungai Citarum. Penelitian ini bertujuan untuk merumuskan rencana lanskap area bersejarah pusat Karawang Kota yang mendukung pelestarian sejarah dan kawasan yang berkelanjutan terhadap perkembangan kota. Metode analisis yang digunakan dalam penelitian ini adalah analisis spasial dan kualitatif pada aspek biofisik fisik, nilai kesejarahan, sosial budaya ekonomi, dan legal. Hasil analisis kesesuaian pengembangan kawasan berdasarkan nilai kesejarahan yang mempertimbangkan nilai kesejarahan-budaya dan kerentananya terhadap banjir, menunjukkan bahwa 33% sesuai, 28% cukup sesuai, dan 39% tidak sesuai. Berdasarkan hasil tersebut, rencana blok dikembangkan dengan membagi tapak menjadi 4 ruang, yaitu konservasi, pemajuan kesejarahan, pengembangan non sejarah, dan penyangga. Konsep perencanaan lanskap untuk kawasan ini adalah untuk menciptakan lanskap yang mengakomodasi kegiatan perkotaan dengan identitas dan karakteristik sejarah yang kuat dengan fungsi konservasi, interpretasi sejarah, dan pelayanan. Rencana lanskap yang dihasilkan dibentuk oleh rencana tata ruang, sirkulasi, fasilitas, dan vegetasi., Karawang is a regency in West Java Province that has historical significance, with historical roots dating back to 414 AD. City center of Karawang underwent changes from various historical periods that made this area continue to develop and has transformed into a historic landscape. Despite the historical values that this area has today, it known as the centers of commerce and the spread of Islam. As the landscape of the city center of Karawang continously grows, it receives tension from surrounding, including housing development and flood given this area is close to Citarum River. This study aims to formulate a landscape plan for the city center of Karawang historical area that supports historical preservation and sustainable area response to the urban development. The analysis method applied in this study spatial and qualitative analysis on the aspects of biophysical physical, historical value, socio-cultural economic, and legal. The analysis of suitability of the area development for a istoric city center which cinsiders historical-cultural significance and its susceptibility to flooding, indicated that 33% of the suitable, 28% quite suitable, and 39% unsuitable. Based on result, block plan was developed in thich it devideds the site into 4 spaces, namely conservation, historical promotion, non-historical development, and buffer. The landscape planning concept for this area was to create a landscape that accommodates urban activities with strong historical identity and characteristics with conservation, historical interpretation, and service functions. The resulted landscape plan was formed by spatial, circulation, facility and vegetation plan.
Keyword: perencanaan lanskap, lanskap kota sejarah, kerawanan banjir

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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