Judul: Viabilitas Embrio Mencit (Mus musculus albinus) setelah Kriopreservasi dengan Vitrifikasi Ganda pada Tahap Perkembangan Zigot dan Dilanjutkan pada Tahap Blastosis.
Abstrak: Development of cryopreservation technology by vitrification method allowed cell storage as a genetic or cell banking that can be utilized in the future. The aim of this research was to find out the viability of mouse embryo after double vitrification at zygote and followed by blastocyst stage. Zygote were obtained from DDY mouse through superovulation by injecting the pregnant mare serum gonadotrophin (PMSG) hormone, followed by human chorionic gonadotrophin (hCG) hormone with interval 48 hours. Before the zygote was being plunged into liquid nitrogen, they were exposed to vitrification medium consist of 15% ethylene glycol + 15% dimethylsulfoxide + 0.5M sucrose in dulbecco’s phosphate buffered saline (DPBS) which supplemented with 20% fetal bovine serum (FBS) and then dropped onto the open inner face of the 0.25 ml straw (hemi-straw carrier). Warming process was done in rehydration medium (sucrose solution leveled by 0.5M, 0.25M, 0.1M in DPBS solution which supplemented with 20% FBS. The result showed that survival rate after double vitrification at zygote and followed by blastocyst stage were 96.81% and 92.50%, respectively, two hours after in vitro culture was similar (P>0.05) compared to control group. The development of hatched blastocyst embryos after single vitrification at blastocyst stage reached 80%, meanwhile after double vitrification reached 66.22%, they were not different (P<0.05) compared to the control group (79.62%). In conclusion, the mouse embryo could be double vitrified at zygote followed by blastocyst stage.
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Judul: Viabilitas Embrio Mencit (Mus musculus albinus) setelah Vitrifikasi dan Kultur In Vitro.
Abstrak: Vitrifikasi merupakan proses kriopreservasi tanpa pembentukan kristal es. Penelitian ini bertujuan untuk mengetahui viabilitas embrio mencit setelah vitrifikasi dan dikultur in vitro. Superovulasi mencit dilakukan dengan penyuntikan PMSG (5 IU / ekor) dan hCG (5 IU / ekor) secara intraperitoneal dengan interval 48 jam. Embrio dikoleksi pada hari ketiga kebuntingan untuk divitrifikasi, dan embrio tanpa perlakuan digunakan sebagai kontrol. Embrio diekuilibrasi pada phosphate buffer saline (PBS) 20% fetal bovine serum (FBS) + 10% ethylene glycol (EG) kemudian dimasukkan ke dalam PBS 20% FBS + 15% EG + 15% dimethyl sulfoxide + 0.5 M sukrosa. Penghangatan dengan menggunakan PBS 20% FBS dan sukrosa 0.5 M, sukrosa 0.25 M dan sukrosa 0.1 M. Pewarnaan Hoesct dan propidium iodide (PI) dilakukan pada embrio setelah 2 hari kultur in vitro. Perkembangan blastosis embrio pasca vitrifikasi dan kontrol yaitu 73.91% dan 85.42%. Total sel embrio pasca vitrifikasi dan kontrol yaitu 99.91±15.87 dan 73.14±9.61 dimana persentase sel hidup yaitu 96.61% dan 97.65%. Tidak ada perbedaan pada perkembangan embrio dan persentase sel hidup. Hasil dari penelitian ini menunjukkan bahwa metode vitrifikasi tidak mempengaruhi viabilitas embrio mencit dan dapat berkembang pada kultur in vitro.
Keyword: embrio, mencit, viabilitas, vitrifikasi, kultur in vitro

Judul: Penerpan Analisis Struktur Jaringan Sosial Pada Jaringan Twitter Akun@matematikaipb.
Abstrak: Twitters is one of the popular social media in cyber space. Each of twitter user has different centrality weights based on its positions i n yhe network structure. The centrality calculations is compared in term of to the number of friendss of users.
Keyword: Twitter, Social Network Analysis, Centrality