Judul: Keragaman Genetik Kemiri Sunan NTT (Reutealis trisperma (Blanco) Airy Shaw) Berbasis Marka RAPD
Abstrak: Reutealis trisperma (Blanco) Airy Shaw atau kemiri sunan menunjukan pertumbuhan dan perkembangan yang cukup menjanjikan di Nusa Tenggara Timur (NTT) yang memiliki lahan kering dan beriklim kering. Untuk memaksimalkan produktivitas rendemen biodiesel dari kemiri sunan maka perlu menggunakan benih yang berasal dari varietas unggul. Program perakitan varietas unggul dengan mengenali keragaman genetik tanaman ini perlu dilakukan untuk memaksimalkan produktivitas kemiri sunan. Tujuan penelitian ini untuk menganalisis keragaman genetik 32 aksesi kemiri sunan dari NTT menggunakan marka Random Amplified Polymorphic DNA (RAPD). Analisis molekuler dilakukan dengan beberapa tahapan: isolasi DNA, pengecekan konsentrasi dan kemurnian DNA, dilanjutkan dengan amplifikasi DNA menggunakan 40 primer, kemudian uji kekerabatan genetik menggunakan program Numerical Taxonomy and Multivariate Analysis System (NTSys) 2.02 dengan metode pengelompokan Sequential Agglomerative Hierarchycal Nested Cluster Analysis (SAHN). Dari hasil penelitian diperoleh 113 pita dengan ukuran berkisar antara 200 bp hingga 3000 bp dan rataan persentase polimorfisme 52.21%. Nilai keragaman genetik menunjukan hasil yang rendah, hanya berkisar antara 0.03 – 0.33. Kemiri sunan aksesi WH2 terpisah pada klaster tersendiri dengan indeks keragaman genetik yakni 0.33, namun belum mencukupi syarat untuk dijadikan sebagai sumber keragaman genetik atau kandidat tetua dalam program perakitan varietas unggul.
Keyword: Kemiri Sunan, Genetic Diversity, RAPD

Judul: Analisis Keragaman Genetik Tanaman Karet Hasil Persilangan antara RRIM 600 dan PN 1546 dengan Menggunakan Teknik RAPD
Abstrak: Low productivity of rubber product had been the main problem in public rubber plantation in Indonesia. New assembly of excellent genotipe rubber plant requires 25-30 years. The more appropriate and accurate method is needed to early determine the new rubber plant clone. This research aimed to shows genetic variation of rubber plants-crossed product between RRIM 600 and PN 1546 with RAPD technique. Two DNA samples from rubber plants parent and 25 DNA samples from rubber plants-crossed products were isolated and amplified by using 16 mTcCIR combination primers and 60 RAPD combination primers. Amplification by using 16 mTcCIR combination primers produced a specific selected marker candidates, while amplification by using 60 RAPD primers produced polymorphism ribbon pattern in agarose gel. Phylogenetic tree was created based on the ribbon to identify the group and family distances of rubber plants-crossed product. Result showed that combination primer ‟d‟ and ‟i‟can be developed into specific marker candidates for RRIM 600. Phylogenetic tree showed that 25 rubber plants-crossed product have 10 high family groups with coeficient 89%. The family group showed that 25 rubber plant-crossed product had high diversity.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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