Judul: Pengaruh Jenis Bahasa Narasi dan Bentuk Pesan Visual Video terhadap Peningkatan Pengetahuan tentang Chikungunya di Kalangan Siswa SMAN 1 Ciampea
Abstrak: Penelitian ini dilaksanakan untuk melihat pengaruh jenis bahasa narasi dan bentuk pesan visual video terhadap peningkatan pengetahuan tentang Chikungunya dikalangan siswa SMAN 1 Ciampea. Penelitian eksperimen ini dilakukan pada 80 siswa yang dipilih secara purposive dan dibagi menjadi empat kelompok perlakuan. Data dianalisa dengan menggunakan uji t-test, analisa sidik ragam dan uji Duncan. Hasil penelitian menunjukkan setelah melihat video, terdapat peningkatan pengetahuan siswa tentang penyakit Chikungunya. Hal ini ditunjukkan dengan adanya perbedaan di antara para siswa, namun perbedaan tersebut tidak signifikan terjadi pada jenis bahasa narasi, yaitu antara bahasa Sunda dan bahasa Indonesia, demikian juga dengan bentuk pesan visual, tidak terdapat perbedaan yang signifikan antara visual realistik dan visual diam sehingga seluruh perlakuan yaitu 4 (empat) kombinasi perlakuan dalam penelitian ini, tidak menunjukkan perbedaan yang nyata pada peningkatan pengetahuan siswa tentang Chikungunya. Meskipun tidak terdapat perbedaan nyata pada semua kombinasi perlakuan, namun 4 (empat) kombinasi perlakuan tersebut efektif digunakan pada penyampaian informasi tentang penyakit Chikungunya.
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Judul: Pengetahuan, Sikap, dan Perilaku Masyarakat Kelurahan Pasir Kuda Kota Bogor terhadap Vektor Penyakit Chikungunya
Abstrak: Chikungunya is a disease which has potentially outbreak in the community. At 2008-2010 there has been a Chikungunya outbreak in Bogor which almost 1.919 cases and in the end of 2010 there has been 41 cases of Chikungunya in Subdistric Pasir Kuda. This study was conducted to measure knowledge, attitudes, and people’s behaviour related to the vector of Chikungunya in Pasir Kuda, and it’s relation to people’s characteristics. Out of 110 respondents were selected randomly, then they were interviewed by using questionares. Questions were consisted of common data of respondent’s characteristic, the knowledge of Chikungunya, the attitude and the people’s behaviour of Chikungunya preventions. The data were analyzed using the SPSS 16.0. The results showed that 110 respodents had a good knowledge, attitude, and behaviour as much as 54%, 90%, and 87%, respectively. The statistics test showed that there were significant difference between the education of respondent to their knowledges (p=0.001,p<0.05), and between the knowledge to their attitudes (p=0.001,p<0.05), but there were not significant difference between the knowledge to their behaviours (p=0.198,p>0.05), and between attitude to their behaviours (p=0.214,p>0.05).
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Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable