Judul: Verification of Determination Ethanol Levels in  Yoghurt Products with SNI 8965:2021 Method
Abstrak: SNI 8965:2021 tentang metode deteksi dan kuantifikasi etanol pada produk minuman merupakan metode standar yang telah divalidasi. Metode standar yang akan digunakan di laboratorium perlu diverifikasi agar metode tersebut dapat dipertanggungjawabkan secara ilmiah, dapat dipercaya, dan hasil uji terjamin sesuai dengan persyaratan dan parameter tertentu. Parameter yang diuji pada penelitian ini ialah akurasi, presisi, ketidakpastian pengujian, rentang kerja, dan limit deteksi metode (MDL) dengan yoghurt sebagai matriks uji. Nilai akurasi diperoleh dengan metode recovery yang menghasilkan data pada rentang 95–105% sehingga data dapat dikatakan akurat. Data akurasi dikatakan presisi karena memenuhi persyaratan nilai Relative Standard Deviation (RSD) < 2/3 CV Horwitz. Nilai MDL aktual yang diperoleh ialah 0,0357 % v/v. Nilai ketidakpastian pada sampel spiking 5% adalah 0,6466 ± 0,0532 5% v/v; nilai tersebut dapat diminimumkan dengan meningkatkan spesifikasi alat dan keterampilan analis. Semua parameter telah memenuhi syarat keberterimaan hasil sehingga metode dapat dikatakan telah terverifikasi., SNI 8965:2021 concerning the method of detection and quantification of ethanol in beverage products is a standard method that has been validated. The standard method that will be used in the laboratory needs to be verified so that the method can be scientifically justified, trusted, and the test results are guaranteed under certain requirements and parameters. The parameters tested in this study were accuracy, precision, test uncertainty, working range, and method detection limit (MDL) with yogurt as the test matrix. The accuracy value obtained by using the recovery method produces data in the range of 95 – 105%, so the data can be said to be accurate. Accuracy is precise because it meets the requirements Relative Standard Deviation (RSD) < 2/3 CV Horwitz. The actual MDL value obtained was 0,0357% v/v. The value of uncertainty in the 5% spiking sample is 0,6466 ± 0,0532 5% v/v, where this value can be minimized by increasing tools specifications and analyst skills. All parameters have met the acceptance requirements of the results so that the method can be said to be verified.
Keyword: accuracy, method detection limit, precision, uncertainty, verification

Judul: Validasi dan verifikasi metode analisis bakteri asam laktat pada produk susu
Abstrak: Lactic acid bacteria (LAB) is a group of bacteria that capable converting carbohydrates (glucose) into lactic acid and mostly grow at facultatif anaerobic condition. At present, the most widely method used to enumerate LAB in some laboratory in Indonesia is incubation in aerob condition. In order to ensure that the method has a good performance, there is a need of method validation. Validation is the confirmation by examination and provision of objective evidence that particular requirements for specified intended use are fulfilled. The purpose of this study is to validate the quantitative method for enumeration of lactic acid bacteria. Two methods of enumeration of LAB in yoghurt were studied, i.e. ISO 7889-2003(E) as a reference method and SNI 2981-2009 as an alternative method. The principle of this method is to grow the facultative anaerobic bacteria in aerobic and anaerobic condition incubated for 3 days at 37 oC on MRSA media. The anaerobic condition was made using Anaxomat jar whose enrich with 10% CO2. The result obtained by these two method were compared. Therefore several parameters such as precision (RSD), relative accuracy, linearity, limit of detection, and limit of quantification were evaluated. The results showed that RSD of these methods between 0.44% and 3.03%, percent recovery of these methods between 97% and 101%, and limit of detection are 3 cfu/mL. It can be concluded that these two methods have good performance characteristics.
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Judul: Perencanan Lanskap Sunda Kalapa, Jakarta Utara Sebagai Kawasan W isata Sejarah
Abstrak: Perkembangan kota Jakarta dari waktu ke waktu sangatlah cepat, sehingga menyebabkan kota-kota tua banyak yang tergusur untuk dijadikan daerah baru yang lebih modern. Apabila hal tersebut tidak ditanggulangi secara dini maka keberadaan Kota Lama Jakarta akan hilang. Hal inilah yang melatar belakang untuk dilakukan tindakan perlindungan dan revitalisasi kawasan bersejarah Sunda Kalapa.
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