Judul: Kualitas Spermatozoa Kauda Epididimis Domba Masa Tumbuh yang Diberi Ekstrak Etanol Daun Katuk pada Penyimpanan 4ºC
Abstrak: Katuk is one of plant that has active compounds which can stimulate reproductive hormone. The purpose of this research was to know the effect of extract katuk leaves in ethanol feed to growth ram on cauda epididymal sperm characteristic. This research is done on six growth local rams around five months old which was divided into two groups, EtOH group and control group. Ram were feed 1500 mg/day of extract katuk leaves in ethanol for two months, where in control group were feed only by placebo. The epididymis were collected after slaughter and the sperm were collected. Collected sperm were analyzed for several sperm characteristic such as concentration, motility, viability, membrane integrity, and abnormality during stored for five days in refrigerator. Sperm concentration and epididymal weight were checked on first day. The result of this research showed there were no significant difference in the sperm concentration in both treatment. Sperm motility, viability, membrane integrity, and abnormality was decreased day by day. However, no significance difference in sperm characteristic in both treatment during storage for five days. The sperm motility was no detected on the day four. Interestingly, the viabilitywere still high and detected until the end of research.
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Judul: Karakteristik dan Kemampuan Fertilisasi In Vitro Spermatozoa Domba yang Berasal dari Kauda Epididimis dan Ejakulat
Abstrak: The characteristic and in vitro fertilization ability of ram spermatozoa collected from cauda epididymides was axamined. Ejaculated spermatozoa was used as control group in this experiment. Characteristic of spermatozoa including of percentage of progressive motility, viability, abnormalities and membrane integrity were evaluated before and after freezing. Fertilization ability of postthawed of the spermatozoa in both group was examined based on the pronucleus formation after in vitro fertilization of in vitro matured (IVM) oocytes. Results from these study showed that there were no significant differences in the characteristics between cauda epididymal and ejaculated spermatozoa before freezing (P>0.05). After freezing, motility of ejaculated spermatozoa was higher than epididymal spermatozoa (54.00±2.24% vs 48.00±4.47%), however the membrane integrity of epididymal spermatozoa was higher than ejaculated spermatozoa (75.38±9.32% vs 65.54±11.88%) (P<0.05). In vitro fertilization experiment revealed that the ability of post-thawed epididymal spermatozoa to fertilize oocytes (61.40%, 42.98%, 18.42% for total, normal and polysperm, respectively) did not differ from that of ejaculated spermatozoa (66.67%, 48.78%, 17.89% for total, normal and polysperm, respectively) (P>0.05). These results indicate that ram spermatozoa collected from cauda epididymal and then frozen have the ability to fertilize ram ooctyes in vitro in the similar rate with ejaculated spermatozoa. The new cryopreservation protocol and reproductive technology describe here can enhance conservation of ram genetic resources.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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