Judul: Pelabelan Super Edge Magic pada Graf Cycle dan Graf Wheel
Abstrak: This manuscript proves that cycle graph and wheel graph have a super edge magic labeling. Super edge magic labeling on a graph is labeling that has an edge magic labeling with a set of vertices were mapped in to {1,2,… ,𝑝} and a set of edges were mapped in to {𝑝+1,𝑝+2,… ,𝑝+𝑞}, in which 𝑝 is order and 𝑞 is size on the graph. There are one lemma and two theorems to be discussed. The lemma is used to prove the two theorems. The first theorem proves that cycle graph 𝐶𝑛 is super edge magic if and only if 𝑛 is odd. The second theorem proves that wheel graph 𝑊𝑛 of order 𝑛 is not super edge magic. Moreover 𝑊𝑛 is not edge magic if 𝑛≡0 mod
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Judul: Pelabelan super edge magic pada graf cycle (P_2n(+)N_m), graf planar ((P_2 U k K_1)+N_m), graf jalinan, dan graf ubur-ubur
Abstrak: Labeling in graph theory is a bijection mapping that maps each elements of a set of vertices and a set of edges to the set of natural number. A graph denoted by ( ( ) ( )) with p vertices and q edges is called a super edge magic if and only if there is a bijective function that maps each of vertices labels to the natural numbers range 1 to and each of edges labels to the natural numbers range to . Also, there is a constant so that the number of two adjacent vertices and one of edges is equal to . In this paper, there are one lemma and four theorems were discussed. The lemma proves that graph with super edge magic labeling has a set of edges that consists of consecutive integers. Each of the four theorems proves that cycle graph ( ( ) ), planar graph (( ) ), braid graph and jellyfish graph has super edge magic labeling. This was proved using the fact indicated in the lemma previously mentioned.
Keyword: Bogor Agricultural University (IPB), Super Edge Magic, Graph Labeling, Consecutive Set of Edges

Judul: Study of Tupaia javanica Hepatocytes Culture as In Vitro Replication Media of Nonhuman Primate HBV
Abstrak: Virus hepatitis B (VHB) adalah virus DNA yang menginfeksi organ hati sebagai target utama. Infeksi ini menyebabkan hepatitis yang dapat bersifat kronis dan berlanjut menjadi sirosis, kanker hati bahkan kematian. Pengembangan model in vivo dan in vitro berkaitan dengan hepatitis B menjadi perhatian khusus bagi para peneliti. Kultur hepatosit banyak digunakan untuk mempelajari berbagai ha] terkait hepatitis B baik dari aspek virus, penyakit, pencegahan, dan pengobatan. Pengembangan hepatosit asal hewan kecil sepe1ii Tupaia sp. untuk model in vitro merupakan salah satu altematif dalam penyediaan kultur hepatosit. Tujuan penelitian ini adalah mengembangkan kultur primer dan lestari hepatosit T. javanica yang mampu mendukung replikasi VHB orangutan (OuHBV) dan VHB owa jawa (GiHBV), dan dapat menjawab kebutuhan akan model in vitro kultur hepatosit. Isolasi hepatosit T. javanica dilakukan dengan prosedur sebagai berikut: organ hati yang telah dicacah dalam cawan petri dengan larutan perfusi, diinkubasi pada 37 °C. Suspensi disentrifugasi 300-500 g sehingga didapatkan pelet sel hati. Pelet yang sudah dicuci kemudian diresuspensi dengan Hepatocyte Basal Media untuk menumbuhkan hepatosit dan selanjutnya hepatosit akan ditransformasi menggunakan perangkat Lenti-SV 40. Sodium taurocholate cotransporting polypeptide (NTCP) yang diindikasikan sebagai reseptor hepatosit untuk melekatnya VHB, nampak pada hepatosit baik pada kultur primer maupun kultur yang ditransformasi. Reseptor hepatosit spesifik ini terlihat sebagai pendaran hijau dengan uji imunofluoresensi. Kultur hepatosit primer T. javanica berhasil dikembangkan selama 14 hari untuk mencapai kepadatan 80%, dan inokulasi GiHBV dan OuHBV ke dalam kultur pada hari ke-15 menunjukkan adanya replikasi virus hingga hari ke-8 (berdasarkan hasil PCR). Kuantifikasi virus dengan real-time PCR memperlihatkan replikasi tertinggi pada hari kedua setelah inokulasi. Penjajaran sebagian protein permukaan setelah virus dinokulasikan memperlihatkan kesamaan asam amino dengan virus wild type sebesar 99.29% dan 95.71 % pada GiHBV dan OuHBV., Hepatitis B virus (HBV) is a DNA virus which infects liver as primary target organ. This i11fection causes hepatitis which could become chronic and progress to cirrhosis, liver cancer and even death. Development of in vitro and in vivo model related to hepatitis B is a concern for researchers. Hepatocyte cultures widely used to study a variety of aspects of hepatitis B such as the virus, disease, prevention and treatment. Development qf hepatocyte from small animal e.g. Tupaia sp. for the in vitro model is an alternative of hepatocyte cultures. The purpose of the study is to develop primary and immortal T. javanica hepatocytes cultures capable to replicate orangutan HBV (OuHBV) and javan gibbon HBV (GiHBV), and to answer the need for in vitro model ofhepatocytes. Isolation of hepatocytes from T. javanica was done as followed: mincedliver were transported into petri dish containing perfusion buffer solution to be incubated at 37 °C. The suspension was centrifuged at 300-500 g to obtain liver cell pellets. The washed-pellet was resuspended in Hepatocyte Basal Media to let specific hepatocytes grow and later be transformed by Lenti-SV40 kit. Sodium taurocholate cotransporting polypeptide (NTCP) has been ident(fied as hepatocyte receptor for HBV attachment, and was seen on hepatocytes from both primary and transformed cultures. This hepatocytes specific receptor was shown as green luminenscent by immunofluorescence assay. Primary T. javanica hepatocytes cultures was successfully maintained for
Keyword: Tupaia javanica, in vitro model, hepatocytes culture, hepatitis B virus, Liver cancer, Picornaviridae, Hepadanaviridae, Flaviviridae, Caliciviridae