Judul: Kualitas Air Cengkareng Drain, Pantai Indah Kapuk, Jakarta Utara Dan Kaitannya Dengan Pasang Surut
Abstrak: Cengkareng Drain merupakan salah satu wilayah yang masih dipengaruhi oleh pasang surut. Perairan Cengkareng Drain dijadikan sebagai titik pengamatan untuk mengkaji keterkaitan antara karakteristik kualitas air dengan pasang surut. Penelitian ini dilakukan satu bulan sekali selama empat bulan pemantauan (Juli-Oktober 2015). Pengambilan contoh air dilakukan pada saat pasang dan surut tiap bulan pemantauan. Indeks Pencemaran menunjukkan bahwa tingkat pencemaran di Cengkareng Drain tergolong tercemar sedang, dengan skor antara 7,7-8,6. Berdasarkan hasil uji t dan analisis similaritas, karakteristik kualitas air pada saat pasang tidak berbeda nyata dengan karakteristik kualitas air pada saat surut. Selama penelitian, nilai total tertinggi pada matrik pembobotan menunjukkan bahwa waktu terbaik pengambilan bahan baku air minum adalah saat surut pada bulan ke-2 pemantauan (Agustus).
Keyword: Cengkareng Drain, kualitas air, pasang surut

Judul: Dynamics of Water Quality and Tides Downstream Cengkareng Drain 2017-2019
Abstrak: Cengkareng drain merupakan salah satu cabang kanal buatan dari sungai Pesanggrahan yang melewati daerah administratif Jakarta Barat dan bermuara di Teluk Jakarta. Muara Cengkareng drain yang terletak di sekitar perumahan menjadikannya sebagai sumber masukan air daerah perumahan Pantai Indah Kapuk (PIK) untuk memenuhi kebutuhan air bersih. Penelitian ini bertujuan menentukan status kelayakan air baku air bersih dengan mendeskripsikan keterkaitan kualitas air di hilir Cengkareng drain, Pantai Indah Kapuk, Jakarta Utara, akibat pengaruh dari pasang surut dan musim. Data yang digunakan merupakan data hasil pemantauan bulanan Tim Kerjasama LPPM IPB – PIK periode 2017-2019. Keterkaitan kualitas air dan pasang surut dianalisis menggunakan analisis regresi linear dan uji t. Pasang surut memberikan pengaruh yang signifikan pada parameter salinitas, DHL, TDS, dan suhu di hilir Cengkareng drain. Air dari hilir Cengkareng drain memerlukan pengelolaan lebih lanjut untuk dapat digunakan sebagai sumber air bersih sesuai baku mutu air Kelas I., Cengkareng drain is a branch of an artificial canal from the Pesanggrahan river that passes through the administrative area of West Jakarta and empties into Jakarta Bay. The Muara Cengkareng drain which is located around the housing estate makes it a source of water input for the Pantai Indah Kapuk (PIK) residential area to meet the community's clean water needs. This study aims to determine the feasibility status of clean water raw water by describing the relationship between water quality in the downstream Cengkareng drain, Pantai Indah Kapuk, North Jakarta, due to the influence of tides and seasons. The data used is the result of monthly monitoring of the LPPM IPB – PIK Cooperation Team for the 2017-2019 period. The relationship between water quality and tides was analyzed using linear regression analysis and t-test. Tides have a significant influence on the parameters of salinity, conductivity, TDS, and temperature in the downstream Cengkareng drain. Water from the downstream Cengkareng drain requires further management to be used as a source of clean water according to Class I water quality standards.
Keyword: Cengkareng drain, dissolved oxygen, salinity, tides, waste

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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