Judul: Study of Community Perceptions and Preferences Regarding the Comfort Level of Merdeka Metro Park as a Social Interaction Space
Abstrak: Pandemi Covid-19 mengakibatkan perubahan perilaku/aktivitas dan ruang gerak sosial masyarakat sehingga memicu perubahan tingkat kenyamanan pengunjung di area taman. Selain itu, kenyamanan termal juga ikut memengaruhi kenyamanan pengunjung dalam beraktivitas sosial di dalam taman. Penelitian ini bertujuan untuk mengidentifikasi dan menganalisis tingkat kenyamanan Taman Merdeka sebagai ruang interaksi sosial berdasarkan persepsi dan preferensi; mengetahui hubungan antara kenyamanan termal dengan kenyamanan taman sebagai ruang interaksi sosial; dan membuat rekomendasi desain Taman Merdeka berdasarkan hasil analisis persepsi dan preferensi masyarakat. Analisis yang digunakan berupa analisis spasial dan analisis deskriptif dengan menggunakan metode berupa uji validitas, uji reliabilitas, skala Likert, skoring, dan perhitungan Temperature Humidity Index (THI) dan daya dukung. Berdasarkan hasil penelitian, terdapat kenaikan nilai THI dari sebesar 2,4 dan penurunan tingkat kenyamanan pengunjung sebesar 0,09 (indeks nilai) sejak sebelum pandemi ke masa pandemi. Terdapat juga perubahan kebutuhan ruang di masa pandemi Covid-19 sebesar dua kali dari daya dukung semula untuk tiap orang per hari. Sehingga rekomendasi yang diberikan di dalam penelitian ini adalah berupa penataan ruang dan tanaman agar tingkat kenyamanan pengunjung taman meningkat., The Covid-19 pandemic has resulted in changes in people's behavior/ social activities space, thus triggering changes in the comfort level of visitors in the park area. In addition, thermal comfort also affects the comfort of visitors in social activities in the park. This study aims to identify and analyze the comfort level of Merdeka Park as a social interaction space based on perceptions and preferences; knowing the relationship between thermal comfort and park comfort as a space for social interaction, and make recommendations for the design of Merdeka Park based on the results of the analysis of people's perceptions and preferences. The analysis was used in the form of spatial analysis and descriptive analysis using methods such as validity test, reliability test, Likert scale, scoring, and calculation of Temperature Humidity Index (THI), and carrying capacity. Based on the results of the study, there was an increase in the THI value from 2.4 and a decrease in the comfort level of visitors by 0.09 (value index) from before the pandemic to the pandemic period. There is also a change in space requirements during the Covid-19 pandemic, which is twice the original carrying capacity for each person per day. So the recommendations given in this study are in the form of spatial planning and plants so that the comfort level of park visitors increases.
Keyword: interaksi sosial, kenyamanan, persepsi masyarakat, preferensi masyarakat, pandemi Covid-19, social interaction, comfort, society perception, society preference, Covid-19 pandemic

Judul: Redesign Of Tegar Beriman Park Cibinong Approach to Pandemi-Friendly Landscape Element Design In Pasca New Normal Era
Abstrak: Penyebaran virus Covid-19 termasuk di Indonesia, menimbulkan permasalahan serius yang merespon dengan penerapan kebijakan pembatasan akses untuk mencegah penyebaran. Kebijakan ini membuat batasan ruang gerak dan interaksi sosial yang berpotensi mempengaruhi kesehatan fisik dan mental manusia, karena pada fitrahnya manusia membutuhkan ruang bebas sebagai bentuk ketenangan, kontemplasi, dan sebagainya. Namun banyak taman kota ditinggalkan selama pandemi karena kurangnya fasilitas. Oleh karena itu, penelitian ini bertujuan merancang ulang Taman Tegar Beriman dengan pendekatan elemen lanskap ramah pandemi, berbasis pada konsep kenormalan baru. Metode yang digunakan adalah analisis spasial untuk mengolah data fisik dan biofisik serta analisis deskriptif untuk mengetahui preferensi pengguna dan kebutuhan masyarakat di era kenormalan baru. Selain itu, penelitian ini berisi rangkaian tahapan desain yang terdiri dari analisis, konsep desain skematik, site plan, dan gambar konstruksi. Konsep dasar dari redesain ini untuk memaksimalkan dan menghidupkan kembali fungsi Taman Tegar Beriman sebagai taman kota dengan penyesuaian kenormalan baru agar masyarakat dapat menikmati ruang terbuka namun tetap merasa aman dari resiko tertularnya virus. Konsep desain yang diambil dari transformasi bentuk virus secara umum dengan pola radial dan garisnya sebagai bentuk ruang dan sirkulasi maupun fasilitas yang ada di dalamnya. Fasilitas pada rencana taman dibuat secara berjarak dan seadaptif mungkin agar dapat menyesuaikan kebutuhan maupun aktivitas pengunjung pada setiap kondisi., The global spread of the Covid-19 including in Indonesia has posed serious problems that are being addressed through the implementation of access restriction policies to prevent its transmission. These policies impose limitations on physical movement and social interactions that potentially affecting both physical and mental health, as humans inherently require open spaces for tranquility, contemplation, and more. However, many city parks have been abandoned during the pandemic due to inadequate facilities. Therefore, this study aims to redesign Taman Tegar Beriman using a pandemic-friendly landscape approach based on the concept of the new normal. The methods employed include spatial analysis to process physical and biophysical data, as well as descriptive analysis to understand user preferences and community needs in the new normal era. Additionally, the research comprises a series of design stages, including analysis, schematic design concepts, site plans, and construction drawings. The basic concept of this redesign is to maximize and revitalize the functions of Taman Tegar Beriman as a city park with adjustments for the new normal, allowing the community to enjoy open spaces while feeling safe from the risk of virus transmission. The design concept is derived from the general transformation of the virus's form, utilizing radial patterns and lines as the spatial and circulation elements, as well as the facilities within. Park facilities are strategically spaced and designed to be adaptive, accommodating the needs and activities of visitors in various conditions.
Keyword: city park, green open space, health protocols, landscape element, pandemic

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.