Judul: Analysis of Land Use Change and Availability of Green Open Space in Kemang Sub-District
Abstrak: Kecamatan Kemang yang terletak di Kabupaten Bogor dan berbatasan langsung dengan Kota Bogor mengalami pertumbuhan penduduk tahun 2010 sampai 2019 dan menurun pada tahun 2020 akibat Pandemi Covid19. Pertumbuhan penduduk dan aktivitas penduduk menyebabkan meningkatnya kebutuhan ruang terbangun yang memicu penyusutan luas ruang terbuka hijau (RTH). Penelitian ini bertujuan menginterpretasi penggunaan lahan, mengidentifikasi perubahan penggunaan lahan, dan menganalisi pengaruh tipe perumahan terhadap luas RTH per desa di Kecamatan Kemang. Interpretasi penggunaan lahan dilakukan secara visual menggunakan citra Google Earth. Analisis perubahan penggunaan lahan dilakukan dengan overlay (SIG) peta penggunaan lahan tahun 2010 dan 2020 serta menggunakan crosstab perubahan penggunaan lahan, dan pengaruh tipe perumahan dan kepadatan penduduk terhadap luas RTH pe desa di analisis dengan regresi linear berganda (stepwise). Hasil penelitian menunjukkan bahwa peningkatan aktivitas mengakibatkan meningkatnya kebutuhan ruang. Penggunaan lahan di Kecamatan Kemang terbagi atas penggunaan lahan terbangun dan tidak terbangun. Proses perubahan mengalami perubahan penggunaan lahan yang signifikan di Kecamatan Kemang dari tahun 2010 sampai 2020. Proses perubahan penggunaan lahan yang terjadi di Kecamatan Kemang merupakan akibat dari alih fungsi lahan tidak terbangun menjadi lahan terbangun sebesar 15,72%. Luas RTH per desa di Kecamatan Kemang dipengaruhi negatif oleh tipe perumahan formal kerapatan tinggi (X3), dan dipengaruhi positif oleh tipe perumahan informal kerapatan sedang (X5), dan kepadatan penduduk (X7)., Kemang District, located in Bogor Regency and directly adjacent to Bogor City, experienced population growth from 2010 to 2019 and decreased in 2020 due to Covid-19 Pandemic. Population growth and activities cause an increase in the need for built space, which leads to green open space depreciation. This study aims to interpret land use, identify land-use changes and analyze housing type affecting the green open space area per village in Kemang District. Land use interpretation is made visually using Google Earth imagery. Land use change analysis was done by overlaying (GIS), land-use maps in 2010 and 2020 using land use change crosstab, and housing effect type and population density on green open space area per village were analyzed using multiple linear regression (stepwise). The results showed that increased activity resulted in increased space requirements. Land use in Kemang District is divided into built and unbuilt land use. Changing process experienced a significant change in land use in Kemang District from 2010 to 2020. The land use change in Kemang District resulted from undeveloped land into built-up land by 15.72%. Green open space area per village in Kemang District is negatively affected by high-density formal housing type (X3), and positively influenced by informal housing of medium-density type (X5), and population density (X7).
Keyword: Population growth, linear regression, and stepwise

Judul: Analysis of Changes in Green Open Space Using SPOT-7 Imagery in Gunung Putri and Klapanunggal Subdistricts, Bogor Regency
Abstrak: Pertumbuhan penduduk menyebabkan penggunaan lahan untuk permukiman, perkantoran dan industri di Kecamatan Gunung Putri dan Klapanunggal meningkat yang berdampak pada perubahan tutupan lahan termasuk luas Ruang Terbuka Hijau (RTH). Penelitian ini bertujuan untuk menganalisis perubahan Ruang Terbuka Hijau menggunakan citra SPOT-7 tahun 2016 dan 2021 dan menganalisis kesesuaiannya dengan Rencana Tata Ruang Wilayah Kabupaten Bogor tahun 2016- 2036. Perubahan luasan RTH pada tahun 2016 hingga 2021 di Kecamatan Gunung Putri dan di Kecamatan Klapanunggal mengalami penurunan. Tetapi luasan RTH tiap kecamatan masih memenuhi peraturan yang telah ditetapkan oleh pemerintah pada daerah perkotaan yaitu sebesar 30%. Kesesuaian Ruang Terbuka Hijau (RTH) dengan Rencana Tata Ruang Wilayah (RTRW) di Kecamatan Gunung Putri terjadi kesesuaian Ruang Terbuka Hijau hanya sebesar 17,28% pada tahun 2016 menurun pada tahun 2021 menjadi 11,53%, sedangkan di Kecamatan Klapanunggal terjadi kesesuaian tinggi yaitu sebesar 84,78% pada tahun 2016 dan 79,54% pada tahun 2021., Population growth causes land use for settlements, offices and industry in Gunung Putri and Klapanunggal Districts to increase which has an impact on changes in land cover including the area of Green Open Space (RTH). This study aims to analyze changes in Green Open Space using SPOT-7 images in 2016 and 2021 and analyze its suitability with the Bogor Regency Spatial Plan 2016-2036. Changes in the area of green open space from 2016 to 2021 in Gunung Putri District and in Klapanunggal District have decreased. However, the area of green open space in each sub-district still meets the regulations set by the government in urban areas, which is 30%. The suitability of Green Open Space (RTH) with the Regional Spatial Plan (RTRW) in Gunung Putri District occurred that the suitability of Green Open Space was only 17.28% in 2016 and decreased in 2021 to 11.53%. Meanwhile, in Klapanunggal Subdistrict, there was a high suitability of 84.78% in 2016 and 79.54% in 2021.
Keyword: Green Open Space, Gunung Putri District, Klapanunggal District, Land Cover, The Regional Spatial Plan of Bogor Regency

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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