Judul: Pemetaan Perubahan Kerapatan Kanopi Hutan di Hutan Rakyat, Kabupaten Kuningan, Jawa Barat
Abstrak: Emisi karbon dioksida dari sektor kehutanan dapat berasal dari deforestasi dan degradasi hutan. Pengukuran deforestasi hutan lebih mudah dilakukan dari pada degradasi hutan. Kerapatan kanopi hutan (Forest Canopy Density/FCD) dapat menjadi variabel penduga dinamika degradasi hutan. Penelitian ini bertujuan untuk mencari hubungan keeratan antara Forest Canopy Density (FCD) dan parameter tegakan yang diukur di lapangan. Penelitian dilakukan di hutan rakyat Kabupaten Kuningan Jawa Barat. Analisis regresi linier sederhana menunjukan koefisisen determinasi terbesar adalah hubungan antara FCD dengan luas bidang dasar tegakan, sebesar 0.71. Persamaan regresi linier hubungan antara FCD dengan LBDT adalah persamaan ini dapat digunakan untuk memprediksi LBDT dengan data FCD. Nilai ketepatan dari model regresi linier ini sebesar 18.98%. Penelitian ini juga bertujuan untuk mengetahui perubahan kerapatan kanopi di hutan rakyat Kabupaten Kuningan pada tahun 1991-2002 dan tahun 2002-2009.
Keyword: degradasi, FCD, hutan, reforestasi, regresi

Judul: Identifikasi Faktor Penyebab Deforestasi dan Reforestasi Ditinjau dari Aspek Sosial Ekonomi (Kasus di KPH Kuningan, Perum Perhutani Unit III Jawa Barat dan Banten)
Abstrak: Deforestation is conversion of forest into other land use/land cover due to forest exploitation, agricultural expansion, settlement development or other activities. Deforestation resulted in wildlife habitat loss and forest cover decline. Meanwhile reforestation is recovery of forest area resulted from natural regeneration or planted. Deforestation and reforestation merely is not happening in the forest or protected forest conservation, but also occur in production forests. Production forests located in Java Island is managed by Perum Perhutani. According Ichsan (2006), in general, the existence of forest is surrounded by villages with social conditions, economic classified as poor. Ownership of land by the community is very narrow, having low technological capabilities, scarcity of capital as well as limited access to public services. These conditions have made the villagers who are living around the forest are difficult to rise from poverty. Moreover revenue contribution of forest land is not optimal for for the community This study aims to determine level of deforestation and reforestation (of natural forests and forest plantations) in KPH Kuningan and identify its socioeconomic factors associated with deforestation and reforestation. The results of this research may be contributed to formulation of programs to reduce deforestation and increase reforestation of Perum Perhutani. This research was conducted in KPH Kuningan start of the month from June to December 2009. The tools used in this study is a set of computers equipped with software Arc View GIS 3.2, ERDAS Imagine 9.1, SPSS 15.0, and Microsoft Office Excel 2007; Global Positioning System (GPS), voice recorder, digital camera; questionnaire (Forest Village Community Forest Village Community Institution); and stationery. The materials used are Landsat 7 ETM (March 2002) and 5 TM (March 2009) path/row 121/65, RBI Indonesia map scale 1: 25.000, the potential of the village in 2006, statistics of Registration Forest Village Community Institution to KPH Kuningan In May 2009, and Recapitulation Results Vulnerability Identification Zone (Red/Green Area Zone) during July- December 2008 KPH Kuningan. In the period 2002-2009, deforestation occurred in Kuningan District of about 16.305,93 ha from total forest area of 29.618,19 ha. There were two villages taken as sample area namely, Sukarapih village with deforestation of - 4.20 ha/year, and Segong Village with reforestation of about 6.77 ha/year. Based on statistical analysis using logistic regression models, it was found that socioeconomic factors have gave significantly affect to deforestation were revenues, forest disturbance, and knowledge about the existence of state forestry logging. While socioeconomic factors significantly affect reforestation was participation in the reforestation of state forestry activities.
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Judul: DNA Extraction and Characterization of Salmonella‟s Lytic Bacteriophage Genome
Abstrak: Bacteriophages are ubiquitous in nature and are known to proliferate wherever their bacterial hosts exist. Their virion particles can exist independently outside the host, however all phages are need their host to propagate. Several phages are highly specific to host cell surface receptors and any slight changes in structure results in little or no interaction between the phage and its host. Salmonella‟s phage isolated from sewage water where their bacteria exist. Three isolated of phages (phages 15, 19 and 38) were used in this research that concentrated using three types of methods. PEG/ NaCl method was firstly used to concentrate the phages based on their molecular weight to precipitate the phage particles during centrifugation. The second method was Scraping Plaque method. In this method, overlay plaques were scraped and collected in SM buffer to get phage concentrates. Enrichment method was the last method used to concentrate the phages. Phage genome isolation was done using two methods i.e. EDTA/SDS and Proteinase K methods. The first method was done using DNase I to eliminate bacterial DNA followed by SDS/EDTA treatment to release phages DNA and phenol-chloroform-isoamyl alcohol to extract the DNA. DNA was collected by isopropanol extraction. While in Proteinase K method, bacterial DNA was eliminated by DNase I and Proteinase K/SDS was used to degrade phages capsids and ethanol for DNA precipitation. The result showed that PEG/NaCl method resulted the highest phage concentration for phage 15 and phage 19, while scrapping method was the highest for phage 38. The quantity and purity of extracted DNA were low for PEG/NaCl and Scraping methods, while Enrichment method was higher indicated higher purity. Run on agarose gel electrophoresis of extracted DNA confirmed that Enrichment method results were the best while scraping and PEG/NaCl method was appeared smear background. Based on DNA genome size determination, the bacteriphages of Salmonella sp were comparable with other study of Salmonella phages. Phage P15 with genome size of 39,5 kb was close to phage epsilon15. Phage P19 with genome size of 41 kb was close to phage ST160. And phage P38 with genome size of 48,5 kb was close to phage Gifsy-1.
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