Judul: Pengaruh Pola Asuh Emosi Orangtua dan Kontrol Diri Remaja terhadap Perilaku Bermain Game Online Remaja Laki-laki dan Perempuan
Abstrak: Penelitian ini bertujuan untuk menganalisis pengaruh pola asuh emosi orangtua dan kontrol diri remaja terhadap perilaku bermain game online pada anak remaja. Penelitian ini melibatkan 60 responden sebagai sampel berusia 16-18 tahun dari SMK Negeri di Kota Bekasi. Pemilihan sekolah dilakukan secara purposive sedangkan pemilihan contoh dilakukan secara non-proportional random sampling. Data dikumpulkan dengan teknik self-report menggunakan kuesioner. Terdapat perbedaan jenis kelamin pada kontrol diri remaja, remaja perempuan memiliki kontrol diri lebih tinggi dibandingkan laki-laki. Pada perilaku bermain game online, remaja laki-laki memiliki perilaku bermain game online yang lebih tinggi dibandingkan perempuan. Hasil uji korelasi menemukan adanya hubungan antara jenis kelamin remaja, pendidikan ibu, dan kontrol diri remaja, dengan perilaku bermain game online pada remaja. Terdapat pengaruh secara langsung oleh kontrol diri remaja dan secara tidak langsung oleh pola asuh emosi melalui kontrol diri terhadap perilaku bermain game online.
Keyword: game online, kontrol diri, pola asuh emosi, remaja, game online, kontrol diri, pola asuh emosi, remaja

Judul: The Effect of Parental Control, Fear of Missing Out (FOMO), and Self-Control towards Junior High School Student's Online Gaming Behavior
Abstrak: Salah satu konten di internet yang sering diakses remaja adalah game online. Penelitian ini bertujuan untuk menganalisis pengaruh kontrol orang tua, FOMO dan kontrol diri terhadap perilaku bermain game online siswa SMP. Desain penelitian adalah cross-sectional study dengan teknik purposive sampling di SMP N 1 Wangon, yaitu siswa kelas 7 yang berusia 12-14 tahun dan merupakan keluarga utuh dengan total responden 200 siswa. Data dikumpulkan dengan teknik self-report melalui pengisian kuesioner. Hasil menunjukan mayoritas siswa mendapatkan kontrol orang tua dalam kategori memadai, FOMO dalam kategori rendah, kontrol diri dalam kategori sedang dan perilaku bermain game online dalam kategori rendah. Terdapat perbedaan signifikan pada tingkat kontrol diri dan perilaku bermain game online antara siswa laki-laki dan perempuan. Kontrol diri dan perilaku bermain game online siswa laki-laki lebih tinggi daripada siswa perempuan. Perilaku bermain game online berhubungan signifikan dan negatif dengan jenis kelamin, kontrol orang tua dan kontrol diri. Perilaku bermain game online juga dipengaruhi secara signifikan dan negatif oleh jenis kelamin dan kontrol diri. Kontrol orang tua dan kontrol diri berpengaruh signifikan secara langsung terhadap perilaku bermain game online, sedangkan FOMO berpengaruh signifikan secara tidak langsung melalui kontrol diri., Game online is one of internet content that often accessed by adolescents. This study aims to analyze the effect of parental control, FOMO, and self-control towards junior high school student’s online gaming behavior. Cross-sectional study was used in this research with purposive sampling technique at SMP N 1 Wangon, that is, 7th grade student aged 12-14 years and came from intact families that consist of 200 students. Data was collected by self-report through filling out a questionnaire. The results showed that majority of students got parental control in medium level, had a low level of FOMO, medium level of self-control and low level of online gaming behavior. There was a significant difference in self-control and online gaming behavior between boys and girls. Boys discovered have higher level of self-control and online gaming behavior than girls. Online gaming behavior significantly correlated with gender, parental control, and self-control. Online gaming behavior significantly influenced by gender and self-control. Parental control and self-control have significant direct effect towards online gaming behavior, meanwhile FOMO has significant indirect effect through self-control.
Keyword: FOMO, junior high school's student, online gaming behavior, parental control, self-control

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable