Judul: Histopathological Changes of The Liver, Kidney, and Spleen in mice (Mus musculus) after Human-Platelet Rich Plasma Injection
Abstrak: Terapi sel berbasis sel manusia di Indonesia memiliki potensi yang tinggi untuk memenuhi kebutuhan unmet medical need. Sel berbasis manusia hanya bisa diujicobakan kepada manusia apabila mampu melewati uji praklinik, dimana sediaan yang diuji harus aman bagi hewan coba. Tujuan penelitian ini yaitu mengetahui pengaruh Human-Platelet Rich plasma terhadap jaringan mencit melalui analisa histopatologi organ hati, ginjal, dan limpa. Sepuluh ekor mencit jantan strain DDY berumur 8 minggu dibagi ke dalam 2 kelompok. Sebanyak 5 ekor mencit diinjeksi dengan human-PRP dengan dosis 4.1 × 10& sel/ gram BB, dan 5 ekor mencit diinjeksi dengan normal saline. Mencit dipelihara selama 14 hari, kemudian dinekropsi dan diambil organ hati, ginjal, dan limpa. Organ dibuat menjadi preparat histopatologi dengan pewarnaan HE. Hasil penelitian yaitu PRP menyebabkan jumlah sel Kupffer menjadi lebih sedikit, hepatosit tidak mengalami piknosis dan sitoplasma menjadi lebih basa, penyempitan ruang bowman ginjal, dan limpa didominasi oleh splenosit immature. Penyuntikan human-PRP tidak menimbulkan efek toksik bagi organ hati, ginjal, dan limpa., Human based cell therapy has a high potential to comply the unmet medical need in Indonesia. Human based cell can only be tested on human only if it has passed the preclinical trial, where the substance is proven to be safe for the animal model. This study aims to find out the effects of human-platelet rich plasma on mice tissue through the analysis of liver, kidney, and spleen histopathology. Ten male DDY strain mice aged 8 weeks were divided into two equal groups. Five mice were injected with human-PRP with 4.1 × 10& cells/ gram weight dose, while the other five were injected with normal saline. Mice were necropsied on the 14th day with the liver, kidney, and spleen collected. The organs were made into histopathology slide and stained with HE under the same condition. The results are reduced numbers of Kupffer cells, non-pyknotic nuclei and alkalized cytoplasm of hepatocyte, narrowed Bowman’s space, and domination of immature splenocytes. These results shows that human-PRP injection had no toxic effects on liver, kidney, and spleen.
Keyword: cell therapy, histopathology, platelet rich plasma

Judul: Histopathological changes of liver, kidney and spleen tissue in mice (Mus musculus) after the application of Xenotransplantation of Human Natural Killer Cell
Abstrak: Sel natural killer (NK) adalah salah satu sel yang banyak digunakan untuk terapi saat ini, dilaporkan berperan dalam pengobatan tumor dan kanker. Penggunaan sel NK sebagai terapi sel memiliki kemungkinan efek toksik pada organ hati, ginjal, dan limpa. Penelitian bertujuan untuk mengkaji respon jaringan organ hati, ginjal, dan limpa mencit (Mus musculus) terhadap uji toksisitas praklinis xenogenik sel NK manusia melalui analisis kuantitatif histopatologi. Sebanyak sepuluh ekor mencit jantan galur DDY umur 8 minggu dibagi menjadi dua kelompok secara acak. Satu kelompok diinjeksi dengan sel NK manusia dosis 4.112 sel/g BB secara intravena dan kelompok yang lain diinjeksi dengan NaCL fisiologis sebagai kontrol. Mencit kemudian dieuthanasia pada hari ke-14 pasca-injeksi, lalu dinekropsi untuk pengambilan organ dan pembuatan preparat histologi. Seluruh proses histologi dan pengambilan gambar mikroskopis dilakukan pada prosedur dan kondisi yang sama. Pengukuran kuantifikasi data histopatologi menggunakan perangkat lunak ImageJ dan analisis statistik menggunakan perangkat lunak R. Hasil penelitian menunjukkan sitoplasma sel hepatosit mengalami perubahan pH menjadi lebih basa, sel hepatosit tidak mengalami piknosis, sel splenosit limpa didominasi oleh sel immature, serta sel tubular ginjal mengalami piknosis tetapi tidak sampai taraf degenerasi. Hasil ini menunjukkan sel NK manusia tidak memberikan efek toksisitas pada ketiga organ mencit., Natural killer (NK) cells are widely used for current therapy, reportedly playing a role in the treatment of tumors and cancer. This study aims to find out the effects of human NK cells on mice tissue through the analysis of liver, kidney, and spleen histopathology. Ten male DDY mice aged 8 weeks were randomly divided into two groups. One group was injected with human NK with 4,112 cells/g weight dose intravenously, while the other group was injected with normal saline as a control. Mice were necropsied on the 14th day post-injection, then the liver, kidney, and spleen collected. The organs were made into histology slide and stained with HE. The whole process of histology and microscopic imaging were carried out under the same procedure and conditions. Measurement of quantification of histopathological data using ImageJ software and statistical analysis using R software. The results showed that the cytoplasm of hepatocyte cells underwent a change in pH to become more alkaline, hepatocyte cells did not undergo pyknosis, splenocytes were dominated by immature cells, and kidney tubular cells underwent pyknosis but did not reach degeneration. These results indicate that human NK cells do not have a toxic effect on the three organs of mice.
Keyword: histopathology, kidney, liver, spleen, NK cells

Judul: Construction of Strongly Optimal Binary Linear Code with Minimum Distance 9 and 11
Abstrak: A binary linear code of length n over q F is a subspace of n q F . A code has three parameters that attached to it, namely length, dimension, and minimum distance. A code with length n, dimension k and minimum distance d is often called [n, k, d ]-code. Usually, when two parameters are given, then we want to find a code that has the best value for the last parameter. Based on Gilbert- Varshamov bound, if a [n, k, d ]-code exists and can not be expanded, we call it a strongly optimal code. In this paper, we construct strongly optimal code with minimum distance 9 and 11. In constructing the code, we created a theorem and algorithm based on Gilbert-Varshamov bound before we implement the algorithm to MAPLE programming language. Because of computational limitations, the program can only construct up to k = 10 for d = 9 and k = 12 for d = 11.
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