Judul: Identifikasi Bakteri Pengoksidasi Metana dan Gen Fungsional Isolat dari Tanah Sawah dan Gambut
Abstrak: Methane is one of green house gases. Methanotrophic bacteria can use methane as their carbon sources, therefore this bacteria has important role to reduce methane emission from soil and wet land. This research aim to isolate methane oxidizing bacteria and identify genes responsible for methane oxidation. Seven isolates of methanotrophic bacterias (AM1, AM2, AM3, AM4, AM5, AM6, dan AM7) were isolated from rice field and wet land through sub-culture in NMS (nitrate mineral salts) added by methane gases method. All of isolates could use 15 mL of methane gases at the third weeks of incubation and 0,7 mL per day in average. Identification of methane monooxygenase (pmoA) gene used two sets primer, 189f - 682r and 189f - 650r. The results showed isolates AM1, AM2, AM4, AM5, and AM6 had pmoA genes. All of the isolates also had functional methanol dehydrogenase (mxaF) gene. The result of 16S rRNA and blast analysis confirmed that six isolates were identified as Methylocystis and one isolate was Mesorhizobium. Phylogenetic analysis shows all strains would be novel species.
Keyword: pmoA gene., mxaF gene, Mesorhizobium, Methylocystis

Judul: Karakterisasi Fisiologi dan Identifikasi Molekuler Isolat-isolat Bakteri Metanotrof Asal Sawah Wilayah Bogor dan Sukabumi
Abstrak: Methane (CH4) is one of the main greenhouse gasses that could absorb infra-red radiation so it contribute to global warming. Methanotrophs are bacteria that are able to grow using methane as their only source of carbon and energy. They play a major role in the reduction of the release of methane into the atmosphere from environments. Methanotrophic bacteria isolated from Ricefields in Bogor and Sukabumi (BGM 1, BGM 2, BGM 3, BGM 9, and SKM 14) had a high methane oxidation. Activity physiologycal assay using API 20 NE and molecular identification using 16S rRNA gene sequence analysis were conducted in this research. Five isolates showed a positive reaction of physiological assays for gelatine, β-galactosidase enzyme, glucose assimilation, mannitol assimilation, N-asetil-glukosamin assimilation, maltose assimilation, and malic acid assimilation. BGM 1 and BGM 3 16S rRNA sequence showed that they had similarity to Methylocystis rosea into 74% and 70% respectively, while BGM 2 and BGM 9 had similarity to Methylococcus capsulatu into 83% and 85% respectively, and SKM 14 had 65% similarity with Methylobacter sp. Phylogenetic tree showed that BGM 1 and BGM 3 isolates were in the same group with Methylocystis rosea strain SV97T, Methylobacterium extorquens, and Methylosinus trichosporium, nevertheless they were very close related to Methylocystis rosea strain SV97T. BGM 2, BGM 9, and SKM 14 isolates were in the same group with Methylococcus capsulatus strain texas, Methylomicrobium buryatense, and Methylobacter sp. nevertheless BGM 2 and BGM 9 isolates were very close related to Methylococcus capsulatus strain texas. While SKM 14 was very close related with Methylobacter sp.
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Judul: Penetapan Harga Jaminan Polis Asuransi Jiwa dengan Premi  Tahunan dan Opsi Surrender
Abstrak: Asuransi jiwa memberikan perlindungan pada tertanggung (pemegang polis) jika mengalami risiko finansial, dengan suatu perjanjian tertanggung harus membayar premi. Premi yang sudah terkumpul pada perusahaan asuransi dapat diinvestasikan, yang salah satu tempatnya adalah di pasar bursa. Perusahaan asuransi menawarkan suatu kontrak kepada pemegang polis yang disebut dengan kontrak partisipasi. Pada kontrak partisipasi risiko finansial dan keuntungan dibagi dengan pemegang polis dan biasanya keuntungan tersebut dimasukkan ke dalam cadangan polis pada tiap akhir tahun kontrak. Hal ini berarti secara tidak langsung perusahaan asuransi menyatakan penambahan pertanggungan, sehingga mengakibatkan perubahan benefit yang diterima oleh pemegang polis. Pada kontrak partisipasi ini, pemegang polis diberikan hak untuk mengakhiri kontrak (opsi surrender). Pada kontrak partisipasi asuransi jiwa endowmen terdapat opsi bonus (bonus option) yang penghitungan besar preminya dengan pendekatan tipe Eropa dan opsi surrender (surrender option) dengan tipe Amerika.
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