Judul: Hubungan antara infertilitas dengan kadar hormon reproduksi pada penduduk pria di Jakarta Raya
Abstrak: Kemandulan bukan hanya disebabkan karena gangguan pada pihak isteri, tetapi juga disebabkan karena gangguan pada suami atau pada keduanya. Untuk mencari jawaban kemandulan karena gangguan pada suami, perlu diselidiki berbagai faktor menjadi penyebabnya. yang mungkin Titik tolak dari penyelidikan ke arah ini dapat mulai dari gangguan sperma suami yang kemudian didapat dilanjutkan pada gangguan yang mungkin menyebabkan timbulnya kemandulan. Penyelidikan ke arah gangguan hormonal menjadi yang menarik terutama dipandang hal dari ketiga hormon repro- duksi yang mempunyai banyak kaitannya seperti testosterone FSH dan LH. Tesosterone yang dihasilkan sel Leydig seperti kita ketahui mempunyai efek langsung dalam produksi spermatozoa melalui pengaruhnya pada sel germinal tubulus seminiferus. FSH mempunyai pengaruh pada sel Sertoli dalam menghasilkan protein khusus yang akan membawa testosterone menuju sel germinal. Gangguan pada salah satu atau ketiganya dengan demikian akan mempengaruhi produksi spermatozoa dan secara tidak langsung berpengaruh pada fertilitas pria.
Keyword: Kemandulan, Sperma suami, Gangguan hormonal, spermatozoa

Judul: Studi Faktor-Faktor Yang Berpengaruh Terhadap Infertilitas Pada Wanita Berkeluarga
Abstrak: Penelitian ini disllsun untuk menelaah faktor sosial ekono~i da[am rumah tangga yang diduga berpengaruh terhadap kasus infertilitas. Penelaahan dilakukan dengan menggunakan analisis CHAID sebagai analisis awal. Untuk mengetahui pengaruh dari faktor-faktor tersebut dilakukan analisis GLM yaitu (I) analisis regresi logistik. dan (2) analisis GLM dengan fungsi hubung log. Hasil dari kedua analisis tersebut memperlihatkan bahwa kecenderungan infertil lebih tinggi apabila termasuk dalam kategori (1) wan ita berstatus bekerja, (2) wanita berpengeluaran tinggi dan mayoritas termasuk ke dalam status bekerja, dan (3) wanita dengan umur kawin pertamanya diatas 40 tahun. Kedua analisis tadi memperlihatkan hasil yang mengarah pada interpretasi yang sarna, tetapi model GLM dengan fungsi hubung log memberikan hasil yang lebih baik dengan devians yang lebih ked!.
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Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable