Judul: Persepsi manfaat dan risiko dalam perilaku pembelian konsumen online shop
Abstrak: The attendance of the internet is used by marketing person to create a new shopping environtment that is e-commerce. One of real forms of the e-commerce is online shop. The purpose of this research was for analizing an example purchasing behaviour. Design of this research was cross sectional study. The election of research location was done with purposively, those are Kaskus, Facebook Online Shop, and Komunitas Online Shop. The pattern election was performed with purposive way that was the consumer who had shopped fashion, book and electronic. The kind of product was most bought by example that does not work that is fashion, meanwhile the example works that is a book. The frequeny of buying of the two example groups are included in low category. The address of online shop was most visited by example that does not work that was Kaskus, meanwhile the example works thas was Facebook online shop. The expense of online shopping by example that does not work including low category, meanwhile for the example that works including very high category. The payment method is had by the example groups was transfer method. Gotten nine factors that become an independent variable those are sex, age, education, job status, income, life style, benefit perception, risk perception, and online searching. Sex, income, benefit perception, and online searching variables are significant factors that influence a purchasing behaviour. Keywords: life style, perceived benefit, perceived risk, searching online, evaluation alternative and purchased behaviour.
Keyword: Persepsi manfaat, Persepsi risiko, Pencarian online, Gaya hidup, Evaluasi informasi, Perilaku pembelian

Judul: Analisis niat pembelian produk melalui Online Shop pada Mahasiswa dengan pendekatan Theory Of Planned Behavior
Abstrak: Perkembangan internet memberikan dampak positif pada bidang perdagangan yang ditandai dengan lahirnya online shop. Sekarang ini, banyak online shop yang berkembang di Indonesia dengan berbagai macam bentuk seperti sebagai media perantara yang mempertemukan penjual dan pembeli di dunia maya. Tujuan penelitian ini adalah menganalisis pengaruh sikap terhadap perilaku, norma subjektif, dan kontrol perilaku yang dipersepsi terhadap niat pembelian di online shop. Penelitian ini mengggunakan design cross sectional-study dengan contoh sebanyak 100 mahasiswa Pascasarjana (S2) IPB yang tercatat aktif sebagai mahasiswa tahun ajaran 2013/2014. Teknik penarikan contoh penelitian ini adalah purposive sampling yang diambil secara proporsional dari 4 fakultas terpilih. Hasil penelitian menunjukkan bahwa karakteristik contoh yang memiliki hubungan dan pengaruh terhadap variabel Theory of Planned Behavior (TPB) adalah pendapatan, intensitas akses internet, jumlah sumber informasi, dan jumlah alasan membuka website. Sikap terhadap perilaku, norma subjektif, kontrol perilaku yang dipersepsi, dan niat pembelian saling berhubungan dan memengaruhi sesuai yang dihipotesiskan. Jadi, dapat dikatakan bahwa penelitian ini sesuai dengan Theory of Planned Behavior., the birth of online shop. Nowadays, many online shops grow in Indonesia on many forms such as online media that bring together the sellers and buyers in virtual world. The purpose of this study was to analyze the influence of attitudes toward behavior, subjective norms, and perceived control behavior toward purchase intentions through online shop. This research used cross-sectional design with 100 postgraduate students (S2) of IPB who are active as a student of 2013/2014 academic year. Sampling technique of this study was purposive sampling taken proportionally from 4 selected faculties. The result showed that the sample’s characterisric who have correlation and influence toward the Theory of Planned Behavior (TPB) variables were income, internet access intensity, mount of information resources, and mount of reasons to access the website. Attitude toward behavior, subjective norm, perceived control behavior, and purchase intention were interconnected and influenced appropriate with the hyphotesis. So, it can be said that this study was consistent with TPB
Keyword: Niat pembelian, Online shopping, Theory of Planned Behavior

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable