Judul: Analisis Sidik Jari DNA Padi Beras Merah, Padi Aromatik, dan Padi Genjah
Abstrak: Rice is one of major crops comodities with a high fenotype diversity. It can be divided into 3 cluster, red rice, aromatic rice, and genjah rice. The differences between those cluster, can be seen from fenotype and genotype of each variety using molecular markers based on gene. The objective of this research is to identify the specific character of red rice, aromatic rice, and genjah rice and the genetic diversity using Simple Sequence Repeat (SSR) molecular markers. Material genetic from rice was isolated using isolation DNA standard method, break the cell wall, expel parts of the cell, and precipitate the DNA parts (Sambrook method), and then the isolate was amplified using polymerase chain reaction (PCR) amplification procedure. Subsequently, analysis by association, evolution, and linkage (TASSEL) software was used for data processing. Genetic variation is based on the result of DNA amplification using PCR process from rice variety used. The results of this research showed the variation of DNA bands from 100 bp until 350 bp of red rice, aromatic rice, and genjah rice varies, also there is association between fenotype and genotype of variety in cluster. TASSEL was used to obtain description of variety and produced a dendrogram that show us the variety type of those variety, also which variety and markers has a significant result in amplification procedure.
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Judul: Identification of Rice Aromatic Properties Using Molecular Aromatic Gene-based Marker.
Abstrak: Rice as a basic commodity can be distinguished between aromatic and nonaromatic. Its different based on gene deletion at chromosom 8. Conventional methods like assessment was method which frequently uses to detect aromatic characteristic of rice. Molecular gene based marker was new method to identify aromatic rice accurately and shorthen analyzing time. The purpose of this research is to distinguish the type of aromatic and nonaromatic variety using aromatic gene-based marker. DNA as rice genetic material was isolated from leaves using CTAB method which broke the cell wall and release the components thus precipitated the DNA. DNA amplification conducted by PCR machine using specific aromatic primer and the result verified by 1.5 % gel agarose electrophoresis. The result of Bradbury primer obtained size of band about 580 bp and 355 bp for nonaromatic, also 580 bp and 257 bp for aromatic. Mentik Wangi and Gunung Perak variety could distinguish to band pattern. Furthermore, RM223 primer can be differentiate both of patterns of aromatic and nonaromatic rice by the band size about 120 bp to 160 bp.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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