Judul: Polymorphism of Prolactin (PRL) Gene Exon 5 and It’s Association with Egg Production on IPB-D1 Chicken
Abstrak: Gen prolaktin pada ayam (PRL) merupakan salah satu gen yang mengontrol sifat mengeram dan produksi telur pada ayam petelur. Sifat yang banyak ditemukan pada ayam lokal yaitu sifat mengeram dan rontok bulu. Sifat ini akan menurunkan produksi telur dan mengganggu sistem reproduksi pada ayam lokal. Tujuan dari penelitian ini yaitu mengidentifikasi keragaman gen prolaktin pada ayam IPB-D1 serta asosiasinya dengan produksi telurnya. Keragaman pada gen prolaktin ekson 5 dilihat pada 112 sampel DNA ayam IPB-D1 betina yang dikoleksi dari Laboratorium Lapangan Genetika dan Pemuliaan Ternak, Fakultas Peternakan IPB. Ekstraksi DNA pada penelitian menggunakan teknik phenol-chloroform. Amplifikasi DNA menujukkan panjang PCR produk sebesar 557 pb. Hasil penelitian menemukan 3 SNP pada gen prolaktin ekson 5 yaitu g.7823 A>G, g.7886 T>A, dan g.8052 T>C. Seluruh SNPs bersifat polimorfik dan berada dalam kesetimbangan Hardy-Weinberg. Hasil analisis asosiasi titik mutasi g.8052T>C berpengaruh secara signifikan terhadap persentase produksi telur harian ayam IPB-D1 dan dapat dijadikan sebagai kandidat marker assisted selection (MAS) sifat produksi telur ayam IPB-D1., The prolactin (PRL) gene is a gene that controls the incubation and egg production in laying chickens. The nature of incubation will reduce egg production and disrupt the reproductive system in local chickens. The purpose of this study was to identify the polymorphism of prolactin genes in IPB-D1 chickens and it’s association with egg production. The polymorphism of the exon 5 prolactin gene was carried out on 112 samples of IPB-D1 chicken deoxyribonucleic acid (DNA) which was a collection of the Division of Animal Genetics and Breeding, Faculty of Animal Science IPB. DNA extraction was carried out using the phenol-chloroform technique. DNA amplification resulted in a polymerase chain reaction (PCR) product with a size of 557 bp. The results found 3 Single Nucleotide Polymorphisms in the PRL gene exon 5, namely g.7835A>G, g.7886A>T, and g.8052T>C. Each mutation was in Hardy-Weinberg’s equilibrium and polymorphic. The SNP association analysis on egg production showed that the point mutation g.8052T>C had a significant effect on the egg production of IPBD1 chickens that potential as a marker assisted selection for the characteristics of egg production in IPB-D1 chickens
Keyword: IPB-D1 chicken, egg production, prolactin gene

Judul: Karakterisasi Gen Prolaktin (PRL) pada Ayam Kampung Ayam Walik Ayam Leher Gundul dan Ayam Arab
Abstrak: Indigenous and local chicken germ plasm in Indonesia have a high potency to be developed. One of the potency that can be developed is an improvement egg production. Low egg production of those chickens can be increased by selecting the in broodiness. Broodiness is controlled by prolactin gene (PRL). Since the characteristics of PRL gene of kampung, walik, leher gundul and arab are still limited refence, therefore this study aim to investigate the characteristic of PRL gene on their. Blood samples were extracted to obtain DNA. PCR then was used to amplified the fragment of PRL. A total of 20 samples successfully amplified at annealing temperature of 60 °C for 45 seconds and then directly sequenced. DNA sequencing results were analyzed using the MEGA 5 program. PRL gene in this study had similarity of 98%-99% with the PRL gene sequence data available on GenBank. This study also shows that there were 11 point mutation of PRL gene’s fragment with the type of mutations were deletions, insertions, transitions and transvertion. Those mutation types have potency as new single nucleotide polymorphisms (SNPs).
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Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4