Judul: Utilization of bone marrow mesenchymal stem cells for therapeutic model of epilepsy
Abstrak: Epilepsy is a chronic neurologic disease, caused by abnormal electrical activity in the brain. Uncontrolled medication using the currently available antiepileptic drugs may result in low quality of life. Bone marrow mesenchymal stem cell (bMSCs), one source of stem cell that is multipotent, can be given as an alternative treatment for intractable epilepsy. This research was aimed to (1) analyze the effect of chemical substance that can damaged the brain, to make a suitable animal model for epilepsy, (2) study of the culture bMSCs, and induced to become neurons in vitro neuron progenitor cells, before it can be transplanted to the animal model, (3) observe the effect of different dosages of bMSCs, and comparing the 2 routes of administration, through an intravenous line or a direct intracerebral approach. This study used 46 Spraque Dawley rats, aged 2 months which were divided to 7 groups consisted 6 rats each (1) negative control group, were given only 1ml NaCl 0.9% intravenous, (2) development of animal group model, 3 rats were given lidocaine 90mg/kg, 3 rats injected with bicuculine 8mg/kg, 1 rat was sacrificed each at the point of 24 hours, 3 days and 7 days after treatment, (3) positive epilepsy group, injected with bicuculine 8mg/kg intraperitoneally, and 3 rats were sacrificed each after 3 weeks and 6 weeks, (4-7) epilepsy group, after injected with bicuculine 8mg/kg intraperitoneally, at day-7 received bMSCs injection, each using 3x2x105 cell intravenous, 1x5x106 cell intravenous, neuron progenitor at 1x2x106 cell intravenous and neuron progenitor at 1x2x105 cell intracerebral. The result of this study showed that bicuculine injection, as a GABA-antagonist, was better when applied to create an epilepsy animal model. The most severe irreversible damaged occurred after the 7th day of treatment. The amount of cells of bMSCs culture at the 4th and 5th passages was sufficient to be transplanted, and the induction of neuron progenitor with basic fibroblast growth factor and epidermal growth factor was seen by noticing the change of the fibroblasts to neurons. The activity of bMSCs to regenerated neurons could be seen that injection of bMSCs 3x2x105 results the highest number of survived neurons. There was no systemic effect of bMSCs. From this study concluded that bMSCs improved regenerate the damage of hippocampal area caused by seizure in epilepsy model animals. A low dose, intravenous administration, given repeatedly every 2 weeks gives a better respon compared to a bolus with high dose intravenous and , intracerebrally. bMSCs treatment was better than progenitor neuron treatment.
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Judul: Quantitative Analysis of Histopathology Changes in Liver, Kidney, and Spleen Tissue of Mice (Mus musculus) after Injection of Human Mesenchymal Stem Cells (hMSCs) and Conditioned Medium (CM)
Abstrak: Terapi stem cell menjadi salah satu metode pengobatan baru yang paling menjanjikan untuk mengobati penyakit degeneratif dan iskemik. Human Mesenchymal Stem Cells (hMSCs) dan Conditioned Medium (CM) dapat menghasilkan respon imun. Penelitian ini bertujuan untuk mempelajari respon jaringan hati, ginjal, dan limpa mencit (Mus musculus) melalui gambaran histopatologi setelah diberi hMSCs dan CM melalui vena coccygea. Mencit DDY jantan dengan jumlah 15 ekor dibagi menjadi tiga kelompok perlakuan, kelompok hMSCs, kelompok CM, dan kelompok kontrol yang diinjeksi normal saline. Mencit dinekropsi pada hari ke-14 dan diambil sampel organ untuk difiksasi dengan formalin 10%, kemudian dibuat preparat histopatologi dengan pewarnaan Haematoxylin Eosin (HE) dengan processing yang sama sehingga warna comparable. Gambar histopatologi di analisa secara kuantatif menggunakan aplikasi ImageJ ver. 1.50g dan R software. Hasil penelitian menunjukkan jumlah sel Kupffer menurun yang mengindikasikan tidak adanya inflamasi, hepatosit diduga mengalami regenerasi, luas ruang Bowman kelompok CM menyempit, sel tubulus kelompok hMSCs lebih mature, dan splenosit kelompok hMSCs dan CM immature. Perubahan tidak memperlihatkan adanya respon toksisitas pada jaringan hati, ginjal, dan limpa mencit., Stem cell therapy is one of the most promising new treatment methods for treating degenerative and ischemic diseases. Human Mesenchymal Stem Cells (hMSCs) and Conditioned Medium (CM) can produce an immune response. This study aims to determine the response of liver, kidney, and spleen tissue of mice (Mus musculus) through histopathology after being given hMSCs and CM through the coccygeal vein. The 15 male DDY mice were divided into three treatment groups, hMSCs, CM, and control injected with normal saline. Mice were necropsied on the 14th day and organ samples were taken to be fixed with 10% formalin, then histopathology preparations were made with Hematoxylin Eosin (HE) staining with the same processing so that the color was comparable. Histopathology images were analyzed quantitatively using the ImageJ app version 1.50 g and R software. The result showed that the number of Kupffer cells decreased which indicated the absence of inflammation, hepatocytes are thought to regenerate, Bowman’s space tightened in the CM group, tubular cells in the hMSCs group were more mature, and splenocytes in the hMSCs and CM groups were immature. The changes did not show any toxic response in the liver, kidney, and spleen tissues of mice.
Keyword: conditioned medium, human mesenchymal stem cells, kidney histopathology, liver histopathology, spleen histopathology

Judul: Distribusi Massa Air Laut Jawa dari Data Hidrografi MAJAFLOX Cruise 2015.
Abstrak: Laut Jawa merupakan perairan dangkal yang dipengaruhi oleh Angin Muson dan Arlindo Selat Makassar. Pemahaman tentang stratifikasi dan distribusi massa air Laut Jawa dapat dilakukan dengan adanya data hidrografi dari MAJAFLOX Cruise pada bulan Agustus 2015. Data hidrografi didapatkan dari penurunan CTD di 14 stasiun pengamatan. Hasil penelitan menunjukkan bahwa stratifikasi vertikal massa air dicirikan oleh lapisan homogen yang kuat dari permukaan hingga dasar perairan. Hal tersebut terjadi akibat percampuran massa air yang kuat oleh aksi gelombang yang dibangkitkan Angin Muson Tenggara. Selain itu, ditemukan variasi spasial dalam arah utara-selatan, dimana karakteristik massa air di tepi selatan dan utara cukup berbeda dengan massa air di tengahnya. Massa air Laut Jawa pada puncak musim timur (Agustus) dicirikan dengan massa air yang hangat (27,18 °C – 28,11 °C), bersalinitas rendah (33,58 psu – 34,2 psu), dan ringan (21,33 kg/m3 – 22,06 kg/m3), kecuali pada stasiun 1 dan stasiun 5 yang cenderung lebih hangat dan lebih tawar dari stasiun lainnya. Pola sirkulasi permukaan menunjukkan salinitas yang relatif tinggi di bagian tengah Laut Jawa dan diduga berhubungan dengan suplai massa air Arlindo Selat Makassar. Variasi tahunan dari analisis keluaran model INDESO menunjukkan adanya stratifikasi massa air yang kuat pada musim peralihan.
Keyword: angin muson, keluaran model INDESO, MAJAFLOX Cruise 2015, pengukuran CTD, stratifikasi massa air