Judul: Optimasi ELISA tidak langsung untuk memeriksa keberadaan antibodi anti Schistosoma japonicum
Abstrak: Schistosomiasis japonicum merupakan zoonosis yang endemik menyerang manusia dan hewan di sekitar lembah Besoa, lembah Napu dan danau Lindu di Sulawesi Tengah, Indonesia. Enzyme Linked Immunosorbent Assay (ELISA) adalah teknik serodiagnosis yang sederhana, sensitif dan mudah digunakan untuk mendeteksi keberadaan antibodi pada penyakit parasitik. Penelitian ini bertujuan menentukan pengenceran serum yang optimal untuk memeriksa keberadaan antibodi anti Schistosoma japonicum pada manusia dengan pengujian ELISA tidak ingsung. Sebanyak 30 serum sampel digunakan dalam penelitian ini, yaitu 15 sampel positif dan 15 sampel negatif. Pengujian dilakukan dengan membandingkan antara pengenceran 1:50 dan 1:100. Hasil penelitian menunjukkan sensitivitas uji, spesifisitas uji dan tingkat akurasi pada pengenceran 1:50 lebih besar dibanding pada pengenceran 1:100 yaitu sebesar 80%, 86.7% dan 83.33%. Hasil menunjukkan bahwa optimasi ELISA tidak langsung yang baik adalah pada pengenceran 1:50. Data hasil penelitian ini juga diharapkan dapat melengkapi hasil penelitian di lapangan sebelumnya….
Keyword: ELISA, Optimization, Schistosoma japonicum

Judul: Optimasi ELISA Tidak Langsung Untuk Memeriksa Keberadaan Antibodi Anti Schistosoma japonicum
Abstrak: Schistosomiasis japonicum was endemic zoonoses that infected human and animals surrounding Besoa valley, Napu valley and Lindu lake in Central Sulawesi, Indonesia. Enzyme Linked Immunosobent Assay (ELISA) was a simple, sensitive, and rapid serodiagnostic technique, which had been widely used for detection antibodies in parasitic diseases. The aim of this study was to determine the optimal serum dilution for detection antibody against Schistosoma japonicum using indirect ELISA. Thirty samples of human serum were used in this research, which 15 samples of positive and 15 samples of negative. The test was compared between 1:50 and 1:100 dilutions. The result showed that the sensitivity 80%, specificity 86.7% and accuracy 83.33% were higher than the 1:100 dilution values. This showed that the best indirect ELISA optimization was the 1:50 dilution. The results were expected to be the complement of the previous study in the field.
Keyword: ELISA, Optimization, Schistosoma japonicum

Judul: Rancangan Kemasan Karton Bergelombang Double Flute untuk Transportasi Buah Belimbing (Averrhoa Carambola L) Varietas Dewi
Abstrak: Star fruit packaging commonly use fruit basket during transportation and stacked usually, whereas it could make mechanical damages to star fruit, like bruised, scratch, and broke on star fruit because it has soft texture. Therefore it needs more special treatment after harvest to reduce the damage during transportation and distribution. The purpose of this research is design a packaging for transportation and distribution on star fruit which the main material are star fruit and corrugated double flute. The steps of this research are designing packaging, compression strength testing and the spread of temperature in cold storage, selecting the packaging, and simulation of transportation. Design process produce four packaging design, that was: the first design with capacity of 24 fruits per package with circle ventilation (K24CV) and with oblong ventilation (K24OV) has dimensions 30 x 23 x 32 cm, then the second design with capacity of 40 fruits with circle ventilation (K40CV) and with oblong ventilation (K40OV) has dimensions 37 x 30 x 32 cm. The material of packaging design used BC flute for primary packaging (outer) and used B flute for secondary packaging (inner). K40CV was a selected packaging based on the testing during research when has average ability to resist compression about 272.32 kgf and more adaptable in cold storage than other packaging design when enter the minute of 130 it has a stable which the different temperature from each other point in the packaging is about 0.15-0.4ºC
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