Judul: Genetic authentication on morphological variation in cultivated pompano (Trachinotus spp.) based on the 16s rRNA gene marker
Abstrak: Ikan bawal (Trachinotus spp.) termasuk komoditas unggulan perairan laut dangkal yang mengalami peningkatan permintaan sehingga perlu dilakukan konservasi melalui kegiatan budidaya. Penelitian ini bertujuan mengautentikasi spesies ikan bawal yang dibudidayakan di BPBL Batam sebagai informasi keragaman genetik dan kekerabatannya. Penelitian dilakukan pada bulan April hingga November 2021. Metode yang digunakan yaitu isolasi dan ekstraksi DNA, amplifikasi dan visualisasi, dan sekuensing gen 16s rRNA. Analisis data meliputi analisis morfologi, pensejajaran, analisis filogeni dan jarak genetik menggunakan MEGA 11. Seluruh sampel menghasilkan DNA total yang baik namun terdapat kontaminan. Hasil PCR menunjukkan pita DNA tunggal dan tebal dengan ukuran basa sesuai target. Hasil validasi menunjukkan seluruh sampel merujuk pada T. blochii dengan nilai persen identifikasi 100% dan nilai jarak genetik 0,000. Pohon filogeni menunjukkan tiga spesies bawal berada satu klaster dengan famili Carangidae. Oleh karena itu, ketiga jenis bawal memiliki kemiripan yang cukup identik dengan spesies T. blochii., Pompano (Trachinotus spp.) is one of the leading commodities in shallow marine waters which is experiencing an increase in demand, so it is necessary to conserve it through aquaculture. This study aims to authentify species of pompano cultivated at BPBL Batam as information on genetic diversity and kinship. The study was conducted from April to November 2021. The methods are using DNA isolation and extraction, amplification and visualization, and 16s rRNA gene sequencing. Data analysis includes morphological analysis, alignment, phylogeny analysis, and genetic distance using MEGA 11. All samples produced good total DNA but contained contaminants. The result of PCR showed DNA single and thick with the base size according to the target. Results of validation showed that all samples referred to T. blochii with a percent identification value of 100% and a genetic distance is 0.000. The phylogeny tree shows that three types of pompano are in one cluster with the family of Carangidae. Therefore it can be concluded that three types of pompano have an identical resemblance to the species of T. blochii.
Keyword: 16s rRNA, genetic, pompano, T. Blochii

Judul: Species Identification in Fisheries Products using Genetic Molecular Marker Cytochrome B Minibarcode
Abstrak: Fish is one of the largest food commodities, so market demand for fish is high. Fish with high economic value is one of the high market demands, this causes fraud in the sale of fish. DNA barcoding is used to identify fishery processed products as well as the authenticity of raw materials so as to reduce food safety problems. The isolation method used was Cetyltrymethyl Ammonium Bromide (CTAB) with concentration and purity testing using a Nanodrop spectrophotometer. The 25 samples consisting of frozen fish fillets, processed fishery product, and salted fish fillets from traditional and supermarket in Jabodetabek. DNA isolates have a concentration of 4,65-3172,6 ng/μL, with a purity of 1,067-2,125. An electrophoregram showed 7 samples sampled on a target DNA of 200-300bp. Analysis using BLAST showed samples detected as Makaira nigricans, Rachycentron canadum, Lates calcarifer, Priacanthus tayenus, Lepidocybium flavobrunneum, and Salmo salar. Phylogeny analysis showed the samples formed a group corresponding to database from Genbank., Ikan menjadi salah satu komoditi pangan yang cukup besar, sehingga permintaan pasar terhadap ikan menjadi tinggi. Ikan dengan nilai ekonomis tinggi menjadi salah satu permintaan pasar yang tinggi, hal tersebut menyebabkan terjadinya kecurangan dalam penjualan ikan. DNA barcoding dengan penanda genetik cytochrome b digunakan untuk mengidentifikasi produk olahan perikanan serta keaslian bahan baku sehingga dapat mengurangi permasalahan keamanan pangan. Metode isolasi yang digunakan adalah Cetyl Trymethyl Ammonium Bromide (CTAB) dengan pengujian konsentrasi dan kemurnian menggunakan spektrofotometer Nanodrop. Sampel berjumlah 25 terdiri dari filet ikan beku, produk olahan perikanan, dan filet ikan asin yang didapat dari pasar tradisional dan swalayan di Jabodetabek. Isolat DNA memiliki konsentrasi 4,65-3172,6 ng/µL, dengan kemurnian 1,067-2,125. Elektroforegram menunjukan 7 sampel teramplifikasi pada DNA target 200-300bp. Hasil analisis menggunakan BLAST menunjukan sampel terdeteksi sebagai Makaira nigricans, Rachycentron canadum, Lates calcarifer, Priacanthus tayenus, Lepidocybium flavobrunneum, dan Salmo salar. Analisis filogeni menunjukan sampel membentuk kelompok yang sesuai dengan basis data dari Genbank.
Keyword: Cytochrome b, DNA barcoding, fishery products, PCR

Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4