Judul: Autentikasi Teri Nasi dari Perairan Selat Madura berdasarkan Biomarka Gen COI Sebagai Dasar Pengelolaan
Abstrak: Ikan teri nasi merupakan salah satu komoditas ekspor Indonesia. Ukuran Ikan yang kecil menyebabkan identifikasi morfologi kurang akurat sehingga diperlukan identifikasi secara biomolekuler untuk kepastian spesies. Penelitian ini bertujuan menentukan spesies ikan teri nasi dari perairan Selat Madura yakni di Pasuruan dan Sampang, Jawa Timur dengan biomarka Gen COI. Penelitian dilakukan pada bulan Januari hingga November 2020. Pengambilan contoh ikan teri dilakukan dengan bantuan nelayan menggunakan alat tangkap payang teri. Analisis molekuler meliputi ekstraksi DNA, amplifikasi DNA dan sekuensing. Berdasarkan hasil identifikasi secara morfologis, ikan teri nasi di perairan Lekok dan Camplong merupakan anggota dari Famili Engraulidae. Adapun secara molekuler berdasarkan gen COI, ikan teri nasi telah teridentifikasi sebagai Encrasicholina heteroloba dan E. pseudoheteroloba. Jarak genetik ikan teri E. pseudoheteroloba antara perairan Lekok dan Camplong adalah 0,007, sedangkan untuk spesies E. heteroloba adalah 0. Nilai jarak genetik ini menunjukkan hubungan kekerabatan yang sangat dekat dari masing-masing spesies teri nasi tersebut di perairan Lekok dan Camplong., Anchovy is one of Indonesia's export commodities. The small size of the fish causes inaccurate morphological identification, so biomolecular identification is required for species certainty. This study aims to determine the species of anchovies from the waters of the Madura Strait, namely in Pasuruan and Sampang, East Java, using COI gene biomarkers. The research was conducted from January to November 2020. A sampling of anchovies was carried out with the help of fishermen using payang anchovies fishing gear. The molecular analysis includes DNA extraction, DNA amplification, and sequencing. Based on the results of morphological identification, anchovy in Lekok and Camplong waters is a members of the Engraulidae family. Molecularly based on the COI gene, anchovy rice has been identified as Encrasicholina heteroloba and E. pseudoheteroloba. The genetic distance for anchovy E. pseudoheteroloba between Lekok and Camplong waters was 0.007, while for E. heteroloba species it was 0. The value of this genetic distance indicated a very close kinship of each anchovy species in Lekok and Camplong waters.
Keyword: Cytochrome Oxidase Subunit I, anchovy, genetic distance

Judul: Molecular identification of the indian mackerel (Rastrelliger kanagurta) based on COI gene markers in Sunda Strait, Cilacap, and Banyuwangi in supporting fisheries management.
Abstrak: Ikan kembung lelaki (Rastrelliger kanagurta) memiliki nilai ekonomis penting bagi nelayan. Ikan pelagis kecil di WPPNRI 572 dan WPPNRI 573 telah mengalami kondisi fully-exploited. Tekanan eksploitasi yang tinggi akan menurunkan keragaman genetik spesies. Penelitian ini bertujuan untuk memastikan spesies, menganalisis kekerabatan dan keragaman genetik secara molekuler ikan kembung lelaki dengan menggunakan marka gen COI di Perairan Selat Sunda, Cilacap dan Banyuwangi. Isolasi dan ekstraksi DNA menghasilkan 30 DNA total, lalu diamplifikasi dengan metode PCR. Hasil sekuensing divalidasi dengan fitur BLASTn pada aplikasi MEGA 11, menunjukkan bahwa 30 contoh dari ke tiga lokasi merupakan spesies R. kanagurta dengan kemiripan antara 98,15% hingga 100%. Jarak genetik ikan kembung lelaki di tiga lokasi memiliki nilai yang rendah. Hasil pohon filogeni menunjukkan contoh memiliki hubungan kekerabatan yang dekat dan adanya pencampuran populasi dari tiga lokasi, sehingga dapat dikelola dalam satu unit pengelolaan., The Indian Mackerel has important economic value for fishermen. The small pelagic fisheries in WPPNRI 572 and WPPNRI 573 have experienced a fully-exploited condition. High exploitation pressure will reduce the genetic diversity of Indian Mackerel species in the wild. This study aims to determine the species, analyze the molecular kinship and genetic diversity of Indian Mackerel (Rastrelliger kanagurta) using COI gene markers in Sunda Strait waters, Cilacap and Banyuwangi. DNA isolation and extraction yielded 30 total DNA, amplified by the PCR method. Sequencing results were validated with the BLASTn feature on the MEGA 11 application, showing that 30 samples were R. kanagurta species with the highest similarity of 100%. The genetic distance of Indian Mackerel in three locations has a low value. The results of the phylogeny tree show that the sample has a close relationship, and there is a mixing of populations from three locations so that it can be managed in one management unit.
Keyword: COI, genetic, Rastrelliger kanagurta

Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4