Judul: Efektivitas Imunoglobulin Y (IgY) Antilipase sebagai Inhibitor Enzim Lipase Pankreas untuk Pencegahan Obesitas
Abstrak: Obesity has been a major risk for many degenerative diseases with an increasing prevalence.Pancreatic lipase is responsible for 50-70% of total dietary fat absorbtion and has been studied widely for new anti-obesity invention. Hen immunized with porcine pancreatic lipase will produce yolk containing IgY antilipase. IgY antilipase will bind the active site of pancreatic lipase in gastrointestinal tract and inhibit the activity of pancreatic lipase in the gastrointestinal tract. This research was conducted to evaluate the effectivity of IgY antilipase as anti-obesity. IgY antilipase was purified with sodium sulphate precipitation. The protein was analysed by SDS-PAGE and was identified by Immunoblotting. The ability of IgY antilipase to inhibit pancreatic lipase was tested by colorimetric microplate assay at five grades of concentration. Yolk containing IgY antilipase was then tested in rabbit to evaluate its ability to inhibit fat absorbtion in vivo. The result showed that IgY contained two proteins with molecular weights of 61.2 kDa and 26.9 kDa. IgY was confirmed because it positively reacted with rabbit-anti-chicken HRP. The average IgY concentration was 30.1 ± 6.3 mg/mL yolk. IgY antilipase and orlistat did not differ in inhibition capacity at various concentrations (p<0.05). The inhibition capacity of IgY obtained from unimmunized hen was significantly lower as compared to those of IgY antilipase and orlistat (p<0.05). Yolk containing IgY antilipase also had the ability to inhibit dietary fat absorbtion in vivo, but its inhibition capacity was lower than that of orlistat (p<0.05). It is concluded that IgY antilipase could be used to inhibit fat absorbtion to prevent obesity.
Keyword: IgY antilipase, orlistat, anti-obesity, pancreatic lipase inhibition, diatery fat absorbtion

Judul: Uji Potensi Inhibisi Ekstrak Daun Sirih Merah terhadap Lipase Pankreas sebagai Antiobesitas secara In Silico
Abstrak: Obesitas menjadi pemicu munculnya berbagai penyakit degeneratif, seperti stroke, jantung, diabetes tipe 2, dan resistensi insulin. Inhibitor lipase pankreas merupakan agen obat antiobesitas dengan kerja menginhibisi enzim lipase pankreas. Penelitian ini bertujuan melakukan pencarian dan prediksi aktivitas inhibisi terhadap lipase pankreas dari senyawa aktif daun sirih merah. Penelitian dimulai dengan penapisan virtual 60 ligan uji, prediksi stabilitas dan toksisitas ligan, validasi penambatan molekuler, penambatan molekuler, dan visualisasi 2D serta 3D. Hasil menunjukkan bahwa benzethonium octyloxyacetate, hydroxybenzoic acid β-D-glucosyl ester, dan anthocyanins memiliki potensi terbaik dalam menginhibisi enzim lipase pankreas berdasarkan penapisan virtual, stabilitas ligan, toksisitas ligan, energi afinitas, jumlah ikatan hidrogen dengan sisi aktif, jarak ikatan, dan konstanta inhibisi (Ki). Benzethonium octyloxyacetate memiliki nilai afinitas sebesar -8 kkal/mol, memiliki interaksi ikatan hidrogen pada residu His349 sisi aktif reseptor lipase pankreas (6KSM) dengan jarak ikatan 2,94 Å, dan nilai Ki sebesar 1,347 μM., Obesity triggers the emergence of various degenerative diseases, such as stroke, heart disease, type 2 diabetes, and insulin resistance. Pancreatic lipase inhibitors are anti-obesity drug agents that work by inhibiting pancreatic lipase enzymes. This study aims to search and predict the inhibitory activity of pancreatic lipase from the active compound of red betel leaf. The research began with virtual screening of 60 test ligands, prediction of ligand stability and toxicity, validation method of molecular docking, molecular docking, 2D and 3D visualization. The results showed that benzethonium octyloxyacetate, hydroxybenzoic acid β-D-glucosyl ester, and anthocyanins had the best potential in inhibiting pancreatic lipase enzymes based on virtual screening, ligand stability, ligand toxicity, energy affinity, number of hydrogen bonds with active site, bond distance, and constants inhibition (Ki). Benzethonium octyloxyacetate has an affinity value of -8 kcal/mol, has a hydrogen bond interaction on the His349 residue on the active site of the pancreatic lipase receptor (6KSM) with a bond distance of 2,94 Å and Ki value of 1,347 μM.
Keyword: molecular docking, pancreatic lipase, obesity, red betel

Judul: Pola Pernikahan Masyarakat Batak Karo di Perantauan
Abstrak: Pernikahan adalah budaya yang tidak akan pernah berhenti, pernikahan akan terjadi secara berkelanjutan Dasarnya, karena pernikahan merupakan kebutuhan biologis manusia. Indonesia adalah negara yang kaya akan budaya dan etnis. Setiap suku di Indonesia mempunyai tata cara/adat istiadat dalam pelaksanaan pernikahan itu sendiri. Begitupun dengan suku Batak karo yang mempunyai sendiri tata cara pelaksanaan pernikahan. Seiring dengan perkembangan zaman Karo Banyak masyarakat yang merantau ke berbagai daerah di Indonesia, salah satunya Bogor berjalan-jalan. Masyarakat Karo yang merantau ke wilayah Bogor pada umumnya akan berusaha melakukan adaptasi ke negara tempat tinggal baru. Keadaan ini juga terjadi pada perkawinan Karo dalam pola adaptasi di Bogor. Namun adaptasi pernikahan masyarakat Karo di Bogor berupaya menjaga nilai-nilai budaya Karo dan sakralisme perkawinan. Mereka do Karo Dimodifikasi pada pola perkawinan, dengan tetap menjaga nilai budaya pernikahan.
Keyword: Weddings Karo, Adaptation marriage patterns, Attitudes