Judul: Preventive Strategies Design of Illegal Indonesian Migrant Workers in Kuala Lumpur, Malaysia
Abstrak: Indonesia merupakan salah satu negara terbesar yang mengirim tenaga kerjanya ke luar negeri. Namun, sebagian besar tenaga kerja tersebut bekerja secara ilegal dimana hak dan kehidupannya tidak terjamin dengan baik. Tujuan dari penelitian ini adalah merancang strategi pencegahan TKI ilegal ke Malaysia. Data yang digunakan dalam penelitian ini adalah data primer dan sekunder. Data primer diperoleh melalui wawancara dan kuesioner. Data sekunder diperoleh dengan mempelajari dokumen seperti buku literatur, hasil laporan penilaian, serta peraturan perundangan-undangan. Data dianalisis dengan Analisis Deskriptif, The House Model dan Pairwise Comparison. Hasil penelitian menunjukan strategi yang menjadi prioritas utama berada pada tahap keberangkatan yaitu (1) Penutupan jalur-jalur keberangkatan TKI illegal, dan (2) Peningkatan sinergitas antara Indonesia dan Malaysia untuk saling mendukung dalam peraturan dan kelengkapan dokumen TKI.
Keyword: Soft System Methodology, Tenaga kerja illegal The House Model

Judul: Competency Strategies Of Indonesian Domestic Workers In Malaysia
Abstrak: ASEAN Economic Community (AEC) is an economic integration in free trade system between ASEAN Countries. The challenges for the Indonesian Governments are to create the stable region, prosperous, highly competitive and realize social welfare. But in fact, Indonesia is facing the problem of low competitiveness among ASEAN countries. This study aimed to (1) analyzing the problematic situation of Indonesian domestic workers in Malaysia; (2) formulating priority strategies to improve the competency of Indonesian domestic workers in Malaysia and (3) porposing a model for improving the competency of Indonesian domestic workers in Malaysia. This research was conducted in Kuala Lumpur and Jakarta from February to June 2016. Experts give the assumptions and analyze value based on certainty and importance. Furthermore, assuming that has a level of interest and high importance and certainty chosen to priority strategies. In this research, choosing the experts are used non-probability method and to collect data are used purposive sampling technique. Data analysis methods used in this research are descriptive analysis, Soft System Methodology based Rich Picture, AHP, and The House Model. The results of the analysis of the problematic situations show that the Indonesian Government is the less concerned with the protection and debriefing training also development to the placement of Indonesian domestic workers in Malaysia. This is caused domestic workers are not able to work in accordance with National work competency standards have been prepared by the Ministry of Manpower of the Republic of Indonesia. It is also reinforced by decision-making method, the Analytic Hierarchy Process (AHP) gives as same as results. Training and development in Indonesia have a higher analysis value than the other alternatives. The important factor is obtained from the AHP is Individual competencies. Therefore, Competency-Based Training is a priority strategy to competitiveness strategies of Indonesian domestic workers in Malaysia with analysis value is 0.361, that will have an impact on the development of individual competencies. The House Model method as the model for improving the competency strategies of Indonesian domestic workers results from the roof with a vision of improving the competitiveness of Indonesian domestic workers in Malaysia within five years (2016-2021). The four strategies factors are the quality of human resource, individual competencies, Government policy, and market demand. Supporting actions are Enhancing the relevant Governmental authorities, Establishing the individual reputation and Collaborating between Government and agencies involved.
Keyword: Analytic Hierarchy Process, Competency, Indonesian Domestic Workers, The House Model, Training and Development

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.