Judul: Analisis Lalu Lintas Harian Rata-rata dengan Kondisi Perkerasan Jalan Raya Jati Asih Kota Bekasi
Abstrak: Peningkatan jumlah kendaraan darat menimbulkan pembebanan berlebih pada jalan yang dilalui kendaraan darat tersebut. Hal ini dapat menimbulkan kerusakan jalan yang merugikan bagi pengguna jalan. Penelitian ini bertujuan untuk menganalisis hubungan antara intensitas arus lalu lintas harian rata-rata dengan tingkat kerusakan jalan dan merancang tebal perkerasan yang baru bila diperlukan. Penelitian ini dilakukan di Jalan Raya Pekayon arah Jati Asih dan Juanda, Bekasi. Perhitungan tebal perkerasan lentur menggunakan metode manual desain perkerasan (MDP) 2013 dengan pemeriksaan metode Pt T-01-2002-B dan metode SNI 03-1732-1989-F. Intensitas arus lalu lintas harian rata-rata yang tinggi yaitu DS = 0.81 merupakan salah satu penyebab kerusakan jalan di kedua arah Jalan Raya Pekayon. Dibandingkan dengan Pt T-01-2002-B dan SNI 03- 1732-1989-F, maka tebal perkerasan yang efektif didapatkan dari metode MDP 2013 dengan tebal lapisan permukaan 150 mm CTB, 150 mm LPA kelas A, 220 mm AC BC serta 50 mm AC WC.
Keyword: intensitas lalu lintas harian, kerusakan jalan, MDP, tebal perkerasan

Judul: Mapping of Road Pavement Conditions on Jalan Raya
Cikampak Cicadas to Jalan Segog Pamijahan
Abstrak: Jalan memiliki peran yang penting dalam menunjang kelancaran aktivitas masyarakat. Oleh karena itu, penelitian ini bertujuan mengidentifikasi jenis dan tingkat kerusakan dari perkerasan jalan dan drainase serta menentukan rekomendasi penanganan untuk perkerasan jalan dengan metode Bina Marga. Hasil dari penelitian menunjukan jenis kerusakan dengan luas permukaan terbesar adalah retak memanjang dengan luas total 582,44 m2, dan retak lateral adalah kerusakan dengan luas permukaan terkecil, yaitu 6,27 m2. Tingkat kerusakan cukup tinggi dialami oleh 18 segmen jalan, yang ditandai dengan perolehan nilai kondisi jalan 7. Rekomendasi berupa pemeliharaan rutin, pemeliharaan berkala dan penambahan secara berturut-turut pada drainase sisi timur 27, 18, dan 10 segmen sedangkan pada drainase sisi barat 35, 14, dan 6 segmen. Rekomendasi penanganan pada jalan diperoleh hasil 10 segmen jalan memerlukan pemeliharaan rutin, 26 segmen jalan memerlukan pemeliharaan berkala dan 19 segmen jalan memerlukan peningkatan dengan biaya penanganan yang diperlukan secara berturut-turut Rp 39.857.105, Rp Rp 302.529.219 dan Rp 1.719.020.425., Roads have an important role in supporting the smooth running of community activities. Therefore, this research aims to identify the type and level of damage to road pavement and drainage and determine treatment recommendations for road pavement using the Bina Marga method. The results of the research show that the type of damage with the largest surface area is longitudinal cracks with a total area of 582.44 m2, and lateral cracks are damage with the smallest surface area, namely 6.27 m2. A fairly high level of damage was experienced by 18 road segments, which was indicated by obtaining a road condition score of 7. Recommendations in the form of routine maintenance, periodic maintenance and successive additions on the east side drainage were 27, 18 and 10 segments while on the west side drainage it was 35, 14, and 6 segments. Recommendations for road handling were obtained as a result of 10 road segments requiring routine maintenance, 26 road segments requiring periodic maintenance and 19 road segments requiring improvement with the required handling costs being Rp 39.857.105, Rp 302.529.219 and Rp 1.719.020.425.
Keyword: drainase, kerusakan jalan, pemeliharaan, jalan

Judul: Introduction and expression of tilapia (Oreochromis niloticus) growth hormone gene in catfish (Clarias sp)
Abstrak: The gene transfer technology applied in commercially important aquatic animals is to enhance genetic quality of aquaculture broodstock. This study was conducted to introduce gene encoding growth hormone (GH) in catfish embryos to improve its growth rate. In fish, microinjection was the earliest technique developed to introduce foreign DNA into fertilized eggs. However, the opaquness, stickiness and buoyancy of the embryos, the invisibility of the pronuclei, the togness of the chorion, and the higher mortality of injected eggs make this technique time consuming and requires sophisticated skills. Electropration method is able to produce mass fish transgenic. In this study, we transferred a plasmid containing GH gene of Nile tilapia (tiGH), driven by medaka β-actin promoter (mBP) into catfish using microinjection and electroporation methods, to obtain growth enhanced transgenic fish. The DNA solution (mBP-tiGH) used was 50 μg/ml in sterile distillated water. The parameter observed was survival rate of embryos (SRe), hatching rate (HR), and the percentage of individual carrying mBP-tiGH. Transgenic individual carrying tiGH was identified by PCR ( Polymerase Chain Reaction) method with specific primer for tiGH gene. The analysis of gene expression was conducted by RT-PCR. The results of research from 100 catfish embryos showed that control uninjected treatment was higher SRe and HR of eggs fertilized while the Sre and HR in electroporated-sperm was similar with control (SRe 98.5%; HR 91.2%). Percentage of catfish carrying tiGH gene by microinjection methods was 42.86% (12/28) while by electroporation methods was 87% and 93%. Germ line transmission of the transgene at first generation was 4.0 % - 8.33%. The growth of catfish in founder generation was not different between transgenic and nontransgenic. The growth of transgenic catfish at first generation were up to 7 fold higher compared with nontransgenic fish.
Keyword: