Judul: Evaluasi Kinerja Sistem Distribusi Air Pada PDAM Tirta Pakuan di Perumahan Griya Melati, Bogor, Jawa Barat
Abstrak: Water is one of the basic requirement for human being. It means that maintenance and menagement of water distribution system gets the first priority. The objective of this research was to evaluate performance of PDAM Tirta Pakuan water distribution system in Griya Melati recidence. The analysis was held based on questioner and Epanet 2.0. Analysis of network capability’s performance was done, including quantity, pressure, continuity, and quality, and also costumer satisfaction about PDAM performance. Based on costumer satisfaction survey about water physical quality parameters (i.e. taste, color and smell), the water was good, even sometime its smell like chlorine. The comparison between water use measurement and Epanet 2.0 simulation showed that PDAM was succeed to fulfill costumer demand even the heads and discharges were fluctuated. The highest headloss on pipe 19 was 0.072 m because the changed of pipe’s diameter from 100 mm to 50 mm so the velocity was increasing. PDAM has been distributing water for 24 hours so the water were always available for customers. Based on water quality test in PDAM laboratory, the result was suitable with Permenkes RI. No. 492/Menkes/Per/IV/2010.
Keyword: clean water, costumer, Epanet 2.0, performance, water distribution system

Judul: Analisis Sistem Distribusi Air Bersih di Perumahan Taman Yasmin Sektor Enam, Bogor, Jawa Barat
Abstrak: Water distribution system is used to distribute water throughout the service area. The objectives of this research was to analyze water distribution system at Taman Yasmin Residence sector six. The analysis was held based on questioner and Epanet 2.0. The result showed that the water quality fulfilled Permenkes No. 492/Menkes/Per/IV/2010. Based on interview about 40 respondents said that water physical quality was suitable. At inlet water discharge was 7,05 l/s so it fulfilled the total of water needs (2,67 l/s). But costumers said that water discharge is smaller in the morning and in the afternoon. The average pressure was 1,9 bar and the inlet pressure was 3,56 bar. So it was suitable with PDAM regulation about maximum pressure on main pipe and secondary pipe (2-4 bar). Based on simulations using Epanet 2.0 there were 4 nodes that had pressure more than 2 bar 3 at tertiary pipe. So it was necessary to install PRV to reduce the pressure on those nodes because the maximum pressure on tertiary pipe was 2 bar. Based on the result of the research it can be concluded that the water distribution system at Taman Yasmin Residance sector six was good and PDAM Tirta Pakuan was expected to increased the discharge in the morning and in the afternoon.
Keyword: distribution system, pressure, discharge, Taman Yasmin Residance sector six, Epanet 2.0

Judul: Cloning and Structure Prediction of C-Type Lectin 19 (CTL-19) from Silkworm (Bombyx mori L.)
Abstrak: Bombyx mori L. is a highly significant insect due to its silk production affects several industries. Unfortunately, B. mori farming has recently experienced mortality issues caused by pathogenic infectious diseases. Despite the extensive study of innate immunity of B. mori, information regarding the molecular functions and mode of actions of C-type lectin 19 (CTL-19) in the innate immunity of B. mori remains limited. Therefore, this study aimed to predict the structure of the B. mori CTL-19 also clone and produce the recombinant protein to better understand its role in the silkworm's innate immunity. The open reading frame (ORF) of the CTL-19 gene (945bp) was successfully amplified and cloned to pGEM®-T Easy Vector. The mature protein consists of 294 amino acids with 2 CRDs, 9 conserved cysteine, and two specific carbohydrate binding motifs. Protein threading demonstrated to be superior in order to predict the ligand binding site in comparison to homology modelling. The predicted molecular weight is 33.26 kDa, with pI value of 5.70. Furthermore, molecular docking analysis reveal that BmCTL-19 have ability to bind to various carbohydrate residues. The BmCTL-19 gene will be sub clone into an appropriate expression vector and produce purified recombinant protein to evaluate its properties.
Keyword: Innate immunity, Carbohydrate Binding Motif, Homology modelling, Protein Threading, Molecular docking