Judul: Struktur Komunitas Ikan Karang pada Ekosistem Terumbu Karang di Pulau Sabira, Kabupaten Kepulauan Seribu, DKI Jakarta
Abstrak: Terumbu karang merupakan ekosistem yang memiliki kompleksitas tinggi karena banyak biota yang berasosiasi di dalamnya, seperti hubungan antara karang keras hidup dengan ikan. Penelitian ini dilakukan untuk mengetahui kondisi ekosistem terumbu karang dan kondisi komunitas ikan karang di Pulau Sabira, Kabupaten Kepulauan Seribu, DKI Jakarta. Pengamatan dilakukan pada tanggal 6-7 Agustus 2019 di empat stasiun pengamatan yaitu Stasiun Utara, Stasiun Barat Laut, Stasiun Barat, dan Stasiun Selatan Pulau Sabira dengan metode Point Intercept Transect (PIT) dan Underwater Visual Census (UVCs). Hasil pengamatan menunjukkan bahwa kondisi tutupan terumbu karang di Pulau Sabira berada pada kategori baik hingga sangat baik dengan persentase tutupan karang hidup tertinggi mencapai 89% yang ditemukan di StasiunUtara. Komunitas ikan di terumbu karang Pulau Sabira berada pada kondisi yang stabil dengan dominansi rendah dan memiliki keanekaragaman sedang
Keyword: Hubungan, Ikan, Komunitas, Pulau Sabira, Terumbu Karang

Judul: Struktur Komunitas Ikan Terumbu di Daerah Budidaya Karang Hias Pulau Panggang, Kepulauan Seribu
Abstrak: Ikan Karang merupakan salah satu indikator dari pertumbuhan terumbu karang. Tujuan penelitian ini adalah mengkaji jenis dan kelimpahan ikan karang serta nilai indeks keanekaragaman, keseragaman dan dominansi di daerah budidaya karang hias pulau Panggang, Kepulauan Seribu. Pengamatan Ikan Karang dan sampel air diambil dari stasiun pengamatan transplantasi karang dalam kondisi baik yang terdiri dari 5 sub-stasiun pada bulan Maret 2014. Ikan karang ditemukan di daerah transplantasi karang perairan pulau Panggang berjumlah 275 individu. Famili yang Nilai rata-rata indeks keanekaragaman pada stasiun pengamatan dalam kategori sedang yang berarti bahwa penyebaran setiap spesies ikan karang sedang sehingga kestabilan dalam komunitas yang terjadi berada dalam kondisi normal. Nilai rata-rata indeks keseragaman pada stasiun pengamatan termasuk dalam kategori keseragaman tinggi dan komunitas stabil. Nilai rata-rata indeks dominansi yang terhitung di stasiun pengamatan berada dalam kategori dominansi yang rendah.
Keyword: Ikan, Transplantasi Karang, Pulau Panggang

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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