Judul: Klasifikasi Vegetasi Sub Zona Pegunungan, Gunung Salak, Bogor Jawa Barat
Abstrak: The research objectives were classifying sub mountain zone of Salak Mountain’s vegetation structurally, physiognomically, floristically; studying the relationship between floristic vegetation types and abiotic factors; and the alteration of structure and species composition in each vegetation type along environmental gradient. Sampling was done with systematic sampling with random start. Vegetation alliances were determined by ordination with factor analysis, and vegetation associations were determined with cluster analysis. Vegetation types at physiognomic and structural level were determined based on UNESCO and NVCS vegetation types. U-Mann Whitney statistic was employed to know whether there were abiotic factors differentiations between alliances and between vegetation structures. Chi-square test was used to know the relationship between vegetation associations and abiotic factors, also the preference of dominant tree species toward abiotic factors. There are three vegetation types founded at alliance levels, which are Schima walichii-Pandanus punctatus/Cinchona officinalis forest alliance (alliance 1); Gigantochloa apus-Mallotus blumeanus/ C. officinalis forest alliance (alliance 2); and Pinus merkusii-Athyrium dilatatum/ Dicranopteris dichotoma forest alliance (alliance 3). Alliance 1 is a broad leaf mix forest dominated by Schima wallichii, alliance 2 is a bamboo forest dominated by G. apus, and alliance 3 is a planted forest dominated by P. merkusii. The abiotic factors that consistently differentiate alliance 1 with the other alliances are N total, dust content of the soil, and slope. P soil’s content for alliance 2, while C organic soil’s content and cation exchange capacity of the soil for alliance 3. Successively, there are 5, 6, and 7 vegetation associations at alliance 1, 2, and 3. Stand structure of alliance 1 and 3 forming J reverse curve. The mean value of dominant tree species diversity and evenness index increases from alliance 2 to 3, and to 1, while the mean value of dominant tree species richness index increases from alliance 3 to 2, and to 1. Each dominant tree species has clumped distribution pattern and uniquely preference to abiotic factor.
Keyword: Cluster Analysis, Alliance, Ordination, West Java, Vegetation Association

Judul: Komposisi Jenis dan Struktur Tegakan Hutan di Taman Nasional Gunung Ciremai, Jawa Barat
Abstrak: INTRODUCTION. The existence of forest as an ecosystem is important for life supporting system. This is because the forest produces various benefits, either in the form of services or goods which are very useful for life. In West Java, as in other parts of Indonesia, forests which are still having relatively good condition, are found only in conservation areas. One of the forest conservation areas which is relatively large is Gunung Ciremai National Park (TNGC). The TNGC constitutes a nature conservation area which possesses important ecological function as life supporting system for the surrounding ecosystem. However, information concerning the existing biodiversity in TNGC is still lacking. Therefore, there is a need for more studies to obtain information on biodiversity level and forest composition and structure in TNGC. METHODOLOGY. This study was conducted from August through September 2008 in altitude zones of < 1.000 m above sea level (asl) (lowland zone), 1.000 – 2400 m asl (montane zone), and > 2.400 m asl (sub alpine zone) in Gunung Ciremai National Park, West Java. This study was performed by using vegetation analysis technique which constitutes combination between strip method for tree stage vegetation inventory and plotted line method for forest regeneration inventory. To ease the inventory process, each strip was divided into plots of 20 m X 20 m for tree stage vegetation inventory, 10 m X 10 m for pole stage inventory, 5 m X 5 m for sapling stage inventory, and 2 m X 2 m for seedling stage inventory. Units of vegetation sample plot were placed in west and south slope directions at altitude zones of < 1.000 m asl, 1.000 – 2.400 m asl, and > 2400 m asl. In each slope direction, two sample units with strip length of 200 m and width of 20 m were established. Sampling units were placed in the field with sampling design of systematic sampling with random start. RESULTS AND CONCLUSION. Sub alpine zone exhibited the least number of species (11 species) at all growth stages, as compared with those of montane zone (62 species) and lowland forest zone (58 species). The number of species in the montane zone was relatively greater as compared with that of lowland forest zone. In lowland zone, the dominant species were huru meuhmal (Litsea tomentosa) at seedling stage (IVI 33,7 %), kileho (Saurauia pendula) at sapling stage (IVI 29,3 %), and pine (Pinus merkusii) at pole and tree stages (IVI 144,7 % and 112.8 %). In the montane zone, the dominant species were huru meuhmal (Litsea tomentosa ) at seedling stage (IVI 22,6 %), Litsea sp. at sapling stage (IVI 19,4 %), walen (Ficus ribes) at pole stage (IVI 32.6 %) and saninten (Castanopsis argentea) at tree stage (IVI 45,4 %). At sub alpine zone, the dominant species was plending (Leucaena glauca) at seedling and sapling stages (IVI 64 % and 76,4 %), cantigi (Vaccinium varingifolium) at pole stage (IVI 124,3 %), and jamuju (Dacrycarpus imbricatus) at tree stage (IVI 138,3 %). Index of species diversity (H’) for all elevation zones in TNGC were categorized as high
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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