Judul: Preferences of Restorative Landscape Elements in Several Urban Parks in Bogor City with Visitor-Employed Photography Approach
Abstrak: Kesehatan dan kesejahteraan manusia dipengaruhi oleh kualitas lingkungan tempat orang tersebut tinggal. Ruang kota harus memenuhi kebutuhan orang yang mencari lingkungan restoratif. Penelitian ini bertujuan untuk mengidentifikasi view lanskap berdasarkan foto geotag, mengklasifikasi elemen lanskap restoratif, serta mengevaluasi efek restorasi pengunjung taman kota. Metode penelitian ini menggunakan tiga pendekatan, pertama analisis spasial sebaran foto dengan QGIS, kedua analisis konten foto menggunakan Google Cloud Vision API, dan terakhir analisis efek restoratif pada responden menggunakan Perceived Restorativeness Scale (PRS). Hasil menunjukkan bahwa terdapat 2-3 hotspot dengan densitas foto paling tinggi di ketiga taman. Hasil dari klasifikasi Cloud Vision API menunjukkan bahwa elemen lanskap restoratif dapat berupa softscape, hardscape, dan faktor lainnya. Hasil uji kuesioner PRS menunjukkan bahwa ketiga taman memberikan efek restoratif kepada pengunjung (p-value < 0.05), Taman Sempur memberikan pengaruh fascination (55.13) dan compatibility (55.57) paling tinggi, serta Taman Heulang memberikan pengaruh coherence (56.85) paling tinggi daripada taman lainnya.
Keyword: Image Annotation, PRS, Restorative Landscape, Urban Park, VisitorEmployed Photography

Judul: Evaluation of Physiological Response from Park Therapy in Heulang Park, Sempur Park, and Bogor Square
Abstrak: Taman kota berfungsi sebagai ruang dengan fungsi terapi yang dirancang untuk memenuhi kebutuhan manusia. Kawasan perkotaan membutuhkan ruang terbuka untuk mendukung aktivitas masyarakat dan menciptakan kesehatan serta keindahan. Keseimbangan antara masyarakat dan alam terganggu di kawasan perkotaan akibat berkurangnya ruang terbuka hijau. Keberadaan taman merupakan upaya mendekatkan hubungan manusia dengan alam yang merupakan salah satu bentuk terapi. Penelitian ini bertujuan untuk mengetahui efek fisiologis dari park therapy, menganalisis persepsi dan preferensi estetika masyarakat terhadap RTH kota Bogor, serta menyusun rekomendasi penataan taman yang berpotensi sebagai taman terapi. Hasil penelitian ini menunjukkan aktivitas jalan kaki di Taman Heulang, Taman Sempur, dan Alun-Alun Kota Bogor berpengaruh terhadap kecenderungan penurunan rata–rata detak jantung pengunjung selama terapi taman serta perbedaan tekanan darah sebelum dan sesudah berjalan di ketiga taman. Masyarakat yang berkunjung memiliki pandangan beragam mengenai kondisi dan kualitas lanskap di ketiga taman. Hasil penilaian terhadap karakter taman dengan nilai terendah meliputi karakter “rumit”, “panas”, dan “monoton”. Sedangkan hasil penilaian terhadap karakter dengan nilai tertinggi di ketiga taman meliputi karakter “menyenangkan”, “terang”, dan “luas”. Rekomendasi disesuaikan dengan kondisi yang dirasakan oleh pengunjung taman serta pemilihan fungsi ekologis vegetasi sehingga memberikan respons fisiologis yang lebih baik dan menghasilkan efek terapi taman yang lebih optimal., City parks work as spaces with therapeutic functions designed to fulfill human needs. Urban areas need open space to support community activities and create public health and beauty. The balance between society and nature is disrupted due to reduced green open space, and the existence of the park is an effort to bring human relations closer to nature. This study aims to determine the physiological effects of Park Therapy, to analyze people's perceptions and preferences of green open space, and to formulate recommendations for the therapeutic parks. The results of this study indicate that walking in the parks have an effect on the tendency to decrease the average heart rate in park therapy process and the difference in blood pressure. People have diverse views regarding landscape condition and quality in the parks. The results of the assessment of the park with the lowest values include "complicated", "hot", and "monotonous". While the results of the assessment with the highest scores in the parks include "fun", "bright", and "spacious". Recommendations are adjusted to the conditions felt by visitors and the selection of the ecological functions of the vegetation to provide a better physiological response and produce a more optimal garden therapeutic effect.
Keyword: Green Open Space, Park Therapy, Physiological Response

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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