Judul: Struktur dan komposisi tegakan serta sistem perakaran tumbuhan pada kawasan karst di Taman Nasional Bantimurung-Bulusaraung, Resort Pattunuang-Karaenta
Abstrak: Plants in Karst Area have different characteristics and distinctive when compared with plants of other forest ecosystem. The purpose of research is to assessing the composition and stand structure and root system of plants in karst areas in Bantimurung-Bulusaraung National Park, Resort Pattunuang-Karaenta. This observation plots determined by purposive sampling based on the representation of the stand. Three sample plots lines with the size of 20 m x 100 m each were constructed in the research site. The research was spread out on four habitats that are on hill base, slopes, summit with considerable soil cover, and summit with very little soil cover. The number of species on the research area is amounted 108 species of seedlings and understorey plants, 108 species saplings, 63 species poles and 78 species trees. Horizontal structure inverted J-shaped and vertical structure of the stand consist of four strata (B, C, D, and E strata). Overall, the spatial distribution patterns have a clumped distribution pattern. Uniform distribution pattern is only found in Palaquium obovatum species of pole on slopes and Dracontomelon dao species of tree on base. Pterospermum celebicum has the highest value of shoot-root ratio on the hill base, index root binding highest value with category of moderate (1.5-3.5) on hill base and index root anchoring highest value with the high category (>1.0) on summits with considerable soil cover.
Keyword: Bogor Agricultural University (IPB), stand structure, root system, stand composition

Judul: Komposisi Jenis dan Struktur Vegetasi pada Kawasan Karst Gunung Cibodas, Kecamatan Ciampea, Kabupaten Bogor.
Abstrak: Karst area is carbonated rock area that showing karst morphology, with high valued ecosystem of flora and fauna. One of the karst areas in West Java that has quite alarming condition is Gunung Cibodas, Ciampea subdistrict, Bogor regency. The purpose of the research is to analyze the composition and structure of vegetation in the karst area of Gunung Cibodas. The location of observation determined by systematic sampling with random start metode. Analysis vegetation plots are defined based on the direction of the slope and the mouth of the cave. The research found 30 species of seedlings, 32 species of saplings, 2 species of poles, 6 species of trees and 92 species of understorey vegetations. On the direction of the slope and the mouth of the cave is dominated by pioneer species such as Leucaena leucocephala, Calliandra calothyrsus and Macaranga peltata which show the process of secondary succession, moreover the understorey is dominated by the species of Invasive Alien Species (IAS). Stand structure inverted J-shaped curve can only be found on the northern slopes and generally canopy stratification in this karst area consists of three strata, namely stratum C, D and E with the dispersion pattern of this species of dominant groups in the direction of the slope and the mouth of the cave.
Keyword: stand structure, karst vegetation, composition of species

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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