Judul: Persepsi, tingkat stres, dan strategi koping ibu pada keluarga miskin penerima bantuan langsung tunai (BLT) terhadap kenaikan harga bahan bakar minyak (BBM) (Kasus di Desa Cikarawang, kecamatan Dramaga, kabupaten Bogor)
Abstrak: Tujuan umum penelitian ini adalah mempelajari persepsi, tingkat stres, dan strategi koping ibu pada keluarga miskin penerima BLT terhadap kenaikan harga BBM di Desa Cikarawang, Kecarnatan Dramaga, Kabupaten Bogor. Tujuan khusus yang ingin dicapai dalam penelitian ini yaitu: (1) mendeskripsikan karakteristik ibu dan keluarga, (2) mendeskripsikan persepsi ibu terhadap kenaikan harga .BBM, (3) mendeskripsikan tingkat stres ibu dengan adanya kenaikan harga BBM, (4) mendeskripsikan strategi koping ibu, (5) menguji hubungan antara karakteristik ibu dan karakteristik keluarga dengan persepsi ibu, (6) menguji hubungan antara persepsi dengan tingkat stres ibu, (7) menguji hubungan antara tingkat stres dengan strategi koping ibu, dan (8) menguji menguji faktor-faktor yang berpengaruh terhadap strategi koping ibu.
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Judul: Perilaku sehat, lingkungan sehat dan coping strategy rumahtangga penerima bantuan langsung tunai (BLT)
Abstrak: Tujuan umum peneliian ini adalah mengetahui dan memperoleh gambaran mengenai pola perilaku sehat, lingkungan sehat serta coping strategy rumahtangga penerima Bantuan Langsung Tunai (BLT) di Kelurahan Cikarawang. Kecamatan Dramaga. Kabupaten Bogor. Tujuan khusus penelitian ini adahh (1) mengidentifikasi karakteristik sosial ekonomi rumahtangga penerima BLT (2) mengidentifikasi kesesuaian antara verifikasi kriteria rumahtangga miskin yang ditetapkan BPS dengan rumahtangga penerima BLT. (3) mengidentifikasi pelaksanaan perilaku sehat individu yang berasal dari rumahtangga penerima BLT. (4) mengidentitikasi keadaan lingkungan tempat tinggal dari rumahtangga penerima BLT. (5) mengidentifikasi coping strategy yang ddakukan untuk mengatasi masahh pangan, non pangan, kesehatan, pendidikan. keuangan dan bahan bakar serta transport akibat kenaikan harga BBM pada rumahtangga penerima BLT, (6) menganalisis hubungan antara karakteristk sosial ekonomi rumahtangga penerirna BLT dengan pelaksanaan pola perilaku sehat lingkungan sehat dan coping strategy.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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