Judul: Analisis Integrasi dan Ekspresi Gen Ketahanan terhadap Geminivirus (AV1) pada Tembakau Transgenik
Abstrak: Geminivirus infection in several vegetable crops such as tomato and chilli has caused leaf curl and yellowing disease. This disease has significantly impact on yield losses of vegetable production in Indonesia. Genetic engineering technology gives an oppurtunity to develop transgenic plant resistant to Geminivirus. Geminivirus AV1 gene expressing coat protein can be used to develop virus resistant plant. The objective of this research was to identify the integration and expression of AV1 gene in tobacco transgenic plant for resistance to Geminivirus. Analysis of AV1 gene integration was carried out by using polymerase chain reaction (PCR) technique and AV1 gene specific primer as well as tobacco genomic DNA as template. Meanwhile, expression analysis of AV1 gene in transcriptional level was conducted by isolating total RNA, cDNA sintesis using reverse transcriptase-PCR (RT-PCR) and amplification AV1 gene product with the cDNAs as a template. Result of this research showed that out of 60 tobacco plants generation T1 there are 36 plants containing AV1 gene and 18 plants of them showed resistant to Geminivirus. Analysis of AV1 gene expression on 6 selected plants showed that 4 plants expressed AV1 gene that indicated by PCR product from cDNA template.
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Judul: Deteksi Geminivirus yang menginfeksi tanaman terung (Solanum melongena L.) dengan teknik Polymerase Chain Reaction.
Abstrak: Diseases caused by whitefly-transmitted Geminiviruses (WTGs) have become serious constraints for crops throughout the world, including Indonesia. WTGs has been reported to cause significant yield loss especially in chili pepper and tomato in Java. This research aims to detect and identify Geminivirus infecting eggplant using polymerase chain reaction (PCR) and nucleotide sequencing. The methods involved DNA extraction using CTAB, DNA amplification and nucleotide sequence analysis by Bioedit V 7.0.5 and ClustalW. Three pair of universal primers, i.e. pAL1v1978/pAR1c715, SPG1/SPG2, pAV494/pAC1048, was used for DNA amplification. Infection of Geminivirus was evidenced from leaves collected from West Java (Bandung) and Central Java (Pati, Rembang, Bantul). Universal primer SPG1/SPG2 was able to amplify DNA fragments of ≈912 bp from all leaf samples. Nucleotide analyses showed that Geminivirus infecting eggplant from West Java and Central Java has a close relationship (98.8%) with Tomato yellow leaf curl Kanchanaburi virus (TYLCVKaV) from Thailand.
Keyword: universal primers., sequence analysis, DNA amplification

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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