Judul: Pemetaan Potensi Hutan Rakyat Menggunakan Teknik Gis Di Kecamatan Sambirejo Kabupaten Sragen Tahun 2014
Abstrak: Peningkatan permintaan kayu menjadi peluang tumbuhnya potensi hutan rakyat. Guna mendukung pengelolaan hutan rakyat berkelanjutan diperlukan ketersediaan data aktual. Penelitian ini mengidentifikasi potensi aktual hutan rakyat di Kecamatan Sambirejo, Kabupaten Sragen melalui teknik inventarisasi berbasis sistem infomasi geografis (GIS). Tujuan utama penelitian ini adalah untuk menghitung, memetakan, dan menganalisis sebaran potensi hutan rakyat menggunakan citra Landsat 8 (OLI). Metode pengambilan data potensi tegakan dilakukan secara purpossive sampling, sedangkan klasifikasi tutupan lahan dan analisis sebaran luas hutan rakyat dilakukan melalui interpretasi visual. Hasil interpretasi visual citra Landsat 8 (OLI) menghasilkan 7 kelas tutupan lahan meliputi badan air, hutan negara, hutan rakyat, lahan terbangun, rumput, sawah, dan tambang. Hutan rakyat memiliki luas lahan sebesar 1307.08 ha dengan nilai potensi per hektar sebesar 58.34 m3/ha dan potensi total sebesar 76 249.67 m3.
Keyword: hutan rakyat, interpretasi visual, pemetaan, potensi tegakan, sistem informasi geografis

Judul: Pemetaan Hutan Rakyat Menggunakan Teknik Sistem Informasi Geografis (SIG) di Kecamatan Talaga Kabupaten Majalengka Tahun 2013
Abstrak: Data dan informasi mengenai tutupan lahan hutan rakyat, baik luas, jenis, jumlah produksi dan harga masih terbatas. Penelitian ini bertujuan untuk memetakan hutan rakyat di Kecamatan Talaga Kabupaten Majalengka menggunakan teknik sistem informasi geografis (SIG) berupa pemanfaatan teknologi citra satelit Landsat 8 OLI. Identifikasi dilakukan secara visual dan uji akurasi menggunakan matrik kontingensi (confusion matrix). Berdasarkan hasil interpretasi diperoleh delapan kelas tutupan lahan yaitu badan air, hutan rakyat, hutan tanaman, pemukiman, pertanian lahan kering, sawah, semak/belukar dan tanah terbuka. Hutan rakyat tersebar di seluruh desa sebesar 819.22 ha. Uji akurasi menyatakan tingkat kesesuaian citra hasil klasifikasi dengan kondisi aktual di lapangan. Semakin tinggi nilai akurasi, maka hasil klasifikasi citra akan mendekati kondisi sebenarnya di lapangan. Hasil uji akurasi menunjukkan nilai overall accuracy sebesar 83.61% dan kappa accuracy sebesar 80.01%.
Keyword: pemetaan, interpretasi visual, hutan rakyat, Kecamatan Talaga

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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