Judul: Landscape Planning of Taman Margasatwa Ragunan Jakarta Based on Bioregion
Abstrak: Taman Margasatwa Ragunan (TMR) mempunyai fungsi sebagai tempat konservasi, edukasi, penelitian, dan rekreasi alam. Sejalan dengan upaya menyejahterakan satwa serta memberikan pengalaman di dunia satwa bagi pengunjung, TMR perlu menata kawasan konservasi satwa ex-situ dengan tema lanskap alami yang menyerupai habitat aslinya. Simulasi habitat yang alami dapat terbentuk dari penataan kawasan berbasis bioregion. Oleh karena itu, diperlukan suatu studi untuk mengetahui karakteristik habitat satwa yang mempengaruhi perencanaan lanskap tersebut. Tujuan dari penelitian ini adalah menyusun rencana lanskap TMR berbasis bioregion. Metode perencanaan lanskap dilakukan dengan survei, analisis deskriptif, dan analisis spasial. Tahap analisis yang dilakukan yaitu menganalisis konsep bioregion melalui analisis karakteristik bioklimat yang merupakan faktor signifikan penyusun bioregion, menganalisis karakteristik eksisting kawasan konservasi ex-situ TMR, dan menyusun rencana lanskap. Hasil penelitian berupa perencanaan lanskap kebun binatang yang berupaya menyediakan ruang untuk mendukung fungsi utama dari kebun binatang tersebut. Perencanaan lanskap TMR ini menghasilkan rencana ruang, rencana aktivitas dan fasilitas, rencana sirkulasi, dan rencana vegetasi., Taman Margasatwa Ragunan has been serviced as a conservation, education, research, and outdoor recreation functions. TMR arranges an ex-situ wildlife conservation area with a natural landscape resembles its natural habitat. This is in line to supports the welfare of its animal, and provide to visitors a total environtmental experience and an opportunity to rediscover the beauty and value of natural fauna and flora in urban oasis. In line with the efforts, TMR needs to organize ex-situ animal conservation areas with natural landscape themes that resemble their natural habitat. Natural habitat simulation can be formed from the arrangement of bioregion-based areas. Therefore, a study is needed to determine the characteristics of animal habitats that affect landscape planning. The purpose of this study is to develop a bioregion-based TMR landscape plan. This landscape planning method is based on a field survey, descriptive and spatial analysis. The results of research in the form of zoo landscape planning which seeks to provide space to support the main functions of the zoo. The TMR landscape planning then produces spatial plans, activity and facility plans, circulation path plans, and vegetation plans.
Keyword: bioregion, ex-situ conservation, habitat simulation, landscape planning, Taman Margasatwa Ragunan

Judul: Bioregion-based  Landcape Planning of Taru Jurug Zoo, Surakarta
Abstrak: Taman Satwa Taru Jurug (TSTJ) merupakan salah satu lembaga konservasi ex-situ yang ada di Indonesia. TSTJ ini merupakan salah satu objek wisata alam yang memiliki fungsi sebagai tempat konservasi, edukasi, dan rekreasi di kota Surakarta. Beberapa lembaga konservasi ex-situ di Indonesia dalam implementasinya belum memperhatikan aspek-aspek yang berkenaan dengan kesejahteraan satwa. Kesejahteraan satwa merupakan hal yang perlu diperhatikan dalam membangun kawasan margasatwa. Sejalan dengan upaya mensejahterakan satwa dan mewujudkan habitat yang sesuai, serta mendukung aktivitas komunitas biota di dalamnya dilakukan penyusunan rencana lanskap TSTJ berbasis bioregion. Metode penelitian ini dilakukan dalam beberapa tahap, yaitu tahap persiapan, tahap inventarisasi, tahap analisis, tahap sintesis dan tahap perencanaan. Analisis dilakukan berdasarkan matriks zona bioklimatik (Jones dan Jones, 2007). Hasil penelitian dituangkan berupa perencanaan lanskap taman satwa yang menyediakan ruang pendukung fungsi konservasi dari taman satwa ini. Perencanaan lanskap TSTJ ini menghasilkan rencana ruang, rencana fasilitas dan aktivitas, rencana sirkulasi, rencana vegetasi, dan rencana satwa eksibisi. Penelitian ini diharapkan dapat menjadi rekomendasi kegiatan konservasi ex-situ yang mempertimbangkan kesejahteraan satwa (animal welfare) di Taman Satwa Taru Jurug.
Keyword: animal welfare, bioclimate, biodiversity, bioregion, ex-situ conservation, landscape planning, animal welfare, bioclimate, biodiversity, bioregion, ex-situ conservation, landscape planning

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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