Judul: Lumut Terestrial dan Ganggang Asosiasinya di Kebun Raya Bogor
Abstrak: Bogor Botanical Garden is an ex-situ conservation which may serve as habitat for bryophyte. Terrestrial bryophyte usually grow on various substrates in shaded habitat or open habitat, such as roadside, along ditches, and rivers. One of the bryophytes benefit in ecosystem is to provide microhabitat for algae. This research was carried out to inventory the bryophyte species grow on terrestrial substrate, and to study their diversity and algal association. The bryophyte were collected on various terrestrial substrates (soil, root, and rock). Quadrates of 25 × 25 cm² were used to sample bryophytes along 30 m transect on three blocks which is dominated by plants with different family, i.e. Meliaceae, Myrtaceae, and Lauraceae to study the diversity. Bryophyte were also sampled used sub-quadrates of 2,5 × 2,5 cm² in the quadrate of 25 × 25 cm² to sample algae associated with bryophyte. Forty three species of terrestrial bryophytes were identified, including 33 species of mosses and 10 species of liverworts. The bryophytes diversity was significantly different when compared to different blocks (p < 0,05). The highest diversity of bryophyte was on block Meliaceae which were more intensively shaded than on any other block. The bryophytes composition was different inter block. Fissidens crassinervis var. laxus, Isopterygium bancanum, and Taxiphyllum cf. taxirameum were dominated on three blocks in Bogor Botanical Garden. These three bryophytes species are commonly grow on soil and roots. Thirteen morphospecies of algae were found in association with terrestrial bryophyte, i.e. Cyanophyta, Chrysophyta, and Chlorophyta.
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Judul: Keragaman dan Kelimpahan Lumut Hati Epifit di Kebun Raya Bogor
Abstrak: Indonesia has a high biodiversity of plants including bryophytes. Studies on the bryophyte diversity of Indonesia has not comprehensively done. Bogor Botanical Garden is one of the ex-situ conservation providing suitable habitat for bryophytes. Studies on the hepaticae of Bogor Botanical Garden are urgently needed. In this studies, bryophyte sampling were done in three blocks where the collection of Meliaceae, Myrtaceae and Lauraceae. Sampling was done based on the difference of growth position of tree (0-100 cm and 100-200 cm above ground) and at the difference of compass point (west, east, south, and north). Specimens checklist of bryophytes in the Herbarium Bogoriense (BO) as well as the herbarium specimens were studied. There are 92 species leafy liverworts are known at the sampling areas in Bogor Botanical Garden and herbarium specimens record at BO. There are 33 species leafy liverworts of epiphyte hepaticae were collected. In this studies, 7 species are recorded as the same as the BO specimens, the rest of 26 species are different with BO specimens. Lejeuneaceae has the highest diversity number, and highest percentage of coverage. Myrtaceae block has the highest diversity number and species abundance of hepaticae, that is 17 species and 52.9% of the total coverage. All of the blocks, position of tree 0-100 cm has the highest species abundance and frequency. A determination key to the species epiphytic hepaticae of Bogor Botanical Garden is presented.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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