Judul: Analisis Harmonik Gelombang Pasang Surut dan Gelombang Permukaan di Teluk Palabuhanratu
Abstrak: Ocean waves such as tides and surface waves affect many activities in marine and coastal area. The purposes of study are to analyze tidal harmonic constituents and wave energy density along with it association with seasonal pattern. Tidal and surface waves measurement held around Sangrawayang, Palabuhanratu using RBR TWR-2050. Recorded data processed utilizing harmonic analysis least square method for tidal wave and distribution and seasonal variability of surface wave. Tidal harmonic analysis show a mixed tides prevailing semidiurnal with Formzahl number is 0.89. Analysis of surface waves shows the wave height ranged between 0.17 - 0.77 meter with dominant period between 10 -15 second categorized as swell. Comparison with Jason-2’s sea surface wave shows there is an association with a seasonal pattern in which the east monsoon wave height trend is higher than the west monsoon.
Keyword: seasonal pattern., harmonic analysis, surface waves, tidal, Palabuhanratu

Judul: A Study On Potential Renewable Energy From Ocean Waves in Palabuhanratu Bay
Abstrak: One of the sources of energy in the sea that can be utilized in Indonesia is wave energy. This study aims to see the potential of ocean wave energy in Palabuhanratu Bay spatially and temporally using a modeling method with the DELFT 3D application. The data sources used are bathymetry data from BIG, wind data from ECMWF, and wave data from Copernicus. Data processing is done by processing bathymetry and wind data first, which is then entered into the model configuration and then adjusted according to the moonson period and scenario. The sea wave power generator (PLTGL) converter scenario is used to estimate the existing potential using a Point Absorber converter. The wind in Palabuhanratu Bay is included in the category of slow gusts with the highest frequency in the category 2,10 – 3,60 m s-1 with a percentage of 30,5%. The sea waves in Palabuhanratu Bay are categorized into Slight Sea with significant wave heights ranging from 0 - 2 m with an average period of 15 seconds. Based on the model results, the wave energy transport has an energy value of up to 0 - 8 kW m-1 with the highest value occurring in the East Season. Based on these results, the Point Absorber type PLTGL produces electric power in Palabuhanratu Bay with two scenarios, the first scenario is in the inner bay which produces 0.09 MW of power with an average energy per day of 261 W. 2,7 MW with an average energy per day of 7344 W.
Keyword: Modelling, Palabuhanratu Bay, Renewable Energy, Waves

Judul: Gene Editing  of Two Cyanide Synthesis-related Genes in Cassava using CRISPR/Cas9
Abstrak: Cassava (Manihot esculenta) contain toxic quantities of cyanogenic glucoside. Reducing the potentially toxic cyanogenic glucoside levels in cassava could be achieved by gene editing through Clustered Regularly Interspaced Short Palindromic Repeats – CRISPR associated protein 9 (CRISPR/Cas9), a high- precision genome editing technique in plants. Two cassava UDP- glucosyltransferase (UGT) paralogs, UGT85K4 and UGT85K5, are the genes catalyzing the synthesis of cyanogenic glucosides in plants. The study aimed to construct the CRISPR/Cas9 recombinant vector carrying single guide RNA (sgRNA) to edit UGT85K4 and UGT85K5 genes, and transform sgRNA CRISPR/Cas9 construct into KU50 cassava calli. Five sgRNAs were designed, considering the on/off target efficiency score and GC content. Those sgRNAs were ligated into pRGEB32 plasmid and introduced to competent Escherichia coli DH5a. The PCR colony and DNA sequencing were performed to verify the sgRNA insert. The amplicons showed the expected band size of recombinant vector (180 bp). Based on sequencing results, only one sgRNA was successfully ligated into the plasmid that will be used to edit the UGT85K5 gene. The sgRNA CRISPR/Cas9 construct in pRGEB32 plasmid was successfully transformed into Agrobacterium tumefaciens and introduced into KU50 cassava calli through co-cultivation. The overgrowth of Agrobacterium occurred after the co-cultivation phase.
Keyword: CRISPR/Cas9, Cyanogenic glucoside, Gene editing, sgRNA