Judul: In Vitro Multiplication and Rooting of Lavender (Lavandula angustafolia) as a Mean of Micropropagation
Abstrak: Lavender merupakan herba potensial pada industri kesehatan, kosmetik, makanan, hingga tanaman hias. Tingginya permintaan industri dapat didukung dengan penyediaan bibit lavender secara in vitro. Penelitian ini bertujuan mengetahui jenis dan konsentrasi sitokinin yang sesuai pada induksi dan multiplikasi tunas, pengaruh BAP terhadap pertumbuhan dan multiplikasi tunas, dan pengaruh konsentrasi NAA pada inisiasi akar. Penelitian dilakukan pada Juni hingga September 2022 dalam tiga percobaan menggunakan Rancangan Kelompok Lengkap Teracak satu faktor. Percobaan 1 induksi dan multiplikasi tunas dengan perlakuan tanpa ZPT, 1 ppm NAA, dan 1 ppm NAA+BAP atau Kinetin (0,3; 0,9 dan 1,5 ppm). Percobaan 2 laju multiplikasi dengan penambahan BAP pada beberapa konsentrasi (0,00; 0,90, dan 0,15 ppm). Percobaan 3 induksi perakaran pada tunas lavender dengan menambahkan beberapa konsentrasi NAA (0,00; 0,25 dan 0,50 ppm). Percobaan 1 menunjukkan kombinasi ½ MS +1 ppm NAA + 0,9 ppm BAP merupakan media terbaik pada induksi dan multiplikasi tunas. Dari percobaan 2 diketahui penambahan 0,9 ppm menginduksi multiplikasi tunas lavender terbaik yaitu 2,6 tunas pada 3 minggu pengkulturan. Percobaan 3 menunjukkan 0,5 ppm NAA merupakan konsentrasi terbaik dalam menginduksi akar dengan jumlah 2,3 akar pada 4 MSK. Penyediaan bibit lavender dapat dilakukan melalui induksi dan multiplikasi tunas dengan perlakuan 0.9 ppm BAP dilanjutkan dengan inisiasi akar pada perlakuan 0,5 ppm NAA secara in vitro. Kata Kunci: BAP, Kinetin , NAA, Organogenesis, Lavender is a herbal plant species widely used for health, cosmetics, beverages and ornamental purposes. The increase in lavender demand can be supported by providing in vitro lavender production. We aimed to determine the best combination of plant growth regulators (PGR) for induction and multiplication of lavender shoot, effect of BAP on shoot growth and multiplication, and the effect of NAA concentration on root initiation. The study was conducted from June to September 2022 in three experiments with a one-factor randomized complete block design. The experiment 1, shoots induction and multiplication were treated without ZPT, 1 ppm NAA, and 1 ppm NAA+BAP or Kinetin (0.3; 0.9 and 1.5 ppm). Experiment 2, BAP validation experiments were performed to observe the shoot multiplication rate. Experiment 3, root induction was carried out with several concentrations of NAA. The first experiment showed MS+ 1 ppm NAA+ 0.9 ppm BAP was the best medium for shoot induction and multiplication. Second experiment was showed that BAP 0.9 ppm had the highest multiplication of shoots with 2,6 shoots at three weeks after culture (WAC). Besides that, MS+ 0.5 ppm NAA was the best for root induction with 2.3 roots in 4 WAC. The lavender seeds provision can be done through shoots induction and multiplication with 0.9 ppm BAP treatment followed by root initiation with 0.5 ppm NAA treatment in vitro. Keywords: BAP, Kinetin, NAA, Organogenesis
Keyword: BAP, NAA, Kinetin, Organogenesis

Judul: Multiplikasi Tunas Suweg (Amorphophallus paeoniifolius (Dennst.) Nicolson) dengan Zat Pengatur Tumbuh BAP dan NAA secara Kultur Jaringan
Abstrak: The utilization of suweg (Amorphophallus paeoniifolius) in Indonesia has been known since the Japanese colonial period as source of carbohydrate. Suweg is beneficial for diabetes, digestion, liver, and increase appetite. The cultivation of suweg is by planting its eye shoot of tuber’s skin, the main eye shoot of tuber, the whole tuber, sapling from the tuber, and seedling. But, there are some problems with that technique which are the habitat where it grows, planting time, and the quality of the result is not uniform. So another cultivation effort is needed which is tissue culture. Based on Isnaini et al. (2012), tissue culture of suweg produces few shoots so it needs further study in order to produce many shoots in a callus. This research was done by the addition of Plant Growth Regulators (PGR) NAA and BAP that is potential for shoot growth. The aim of this study is to determine the effect of BAP and NAA in suweg shoot multiplication. This research was conducted at the Laboratory of Plant Tissue Culture Conservation Centre Bogor Botanical Garden, from June until September 2012. The research was designed by using a two-factor factorial experimental design on the basis of completely randomized design (CRD). The first factor is the concentration of BAP with four levels which are 0 mg/l, 1 mg/l, 2 mg/l, and 3 mg/l. The second factor is the NAA concentrations with four levels which are 0 mg/l, 0.25 mg/l, 0.5 mg/l, and 0.75 mg/l. Observed was done to explants browning, contamination (bacteria and fungi), callus color, callus texture, number of shoot, and the number of roots. The result shows the percentage of successful explant survival is 94.17% or 226 explants. Contamination by bacteria and fungi are 2.08 or 5 explants, and browning are 3.75% or 9 explants. Explant with compact texture is 34.58% and explant with crumb texture is 63.33%. The most root growth was found in the treatment of 1 mg /1 BAP + 0.75 mg/l NAA is 2.87 root/ week. The highest number of shoot in the treatment of 1 mg/l BAP + 0.25 mg/l NAA is 0,47 shoot/ week. The conclusion of this study is that the shoot multiplication was occurred in three explants, which are in the treatment of 1 mg l BAP + 0.25 mg/l NAA, 3 m/l BAP + 0.5 mg/l NAA and 3 mg/l BAP + 0.75 mg/l NAA.
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Judul: Metrik Einstein (Anti-) Self-Dual Berdimensi Empat dengan Metode Cartan
Abstrak: Gravitational field equation can be formulated in differential forms (differential geometry), which describes gravity as a manifestation of the curvature of space-time. Curvature of space-time is characterized by a metric that satisfies the gravitational field equation. With differential forms it can be formulated the (anti-) self-dual Einstein equation, which is a modification of the Cartan structure in (anti-) self-dual basis. Calculation of (anti-) self-dual metric with (anti-) self-dual Einstein equation is simpler than the calculation using Christoffel symbols.
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