Judul: Inisiasi Rimpang Mikro Kaempferia parviflora Secara In Vitro dengan Penambahan BAP dan Sukrosa
Abstrak: Kaempferia parviflora is a medicinal plant that contains secondary metabolites and efficacious for treating various diseases. Rapid multiplication of K. parviflora through its rhizome is hamprered by the long period needed to produce the rhizome. The objectives of this research were to obtain the best sucrose concentration and to determine the best BAP concentration in the in vitro micro rhizome initiation of K. parviflora. The experiment was arranged in a randomized complete block design with two factors and three replications. The first factor was BAP concentration, consisted of three levels i.e. 0, 2, and 4 mg L¯1. The second factor was sucrose concentration, consisted of four levels i.e. 0, 30, 60, and 90 g L¯1. Data obtained were analyzed by F-test, followed by DMRT (Duncan Multiple Range Test) at 5 % significant level. Sucrose concentration significantly affected the number of shoots and plantlets fresh weight. The results showed that K. parviflora could form micro rhizome in vitro, as indicated by the similar anatomical structure compared to the rhizome produced in vivo. K. parviflora cultured in MS medium cointaining 90 g L¯1 sucrose without BAP had the highest number of micro rhizome at 8 WAC.
Keyword: Bogor Agricultural University (IPB), traditional medicine, black galingale, health

Judul: In Vitro Shoot Induction of Kaempferia parviflora Wall. Ex. Baker
Abstrak: This research aimed to find the best MS salt concentration and cytokinin (BAP) concentration for K. parviflora rapid multiplication by using in vitro buds as explants. The experiment was done at Plant Tissue Culture Laboratory of Agronomy and Horticulture Department, Faculty of Agriculture, Bogor Agricultural University from January to November 2011. This research began by sterilizing shoots of K. parviflora rhizomes received from PT. Ogawa Indonesia to produce axenic culture. After about 8 weeks of growth, young shoots began to sprout. The young shoots of K. parviflora axenic culture were then cut and cultured individually on the treatment medium, which consist of two different MS salt concentrations (MS and ½ MS) and five different 6-benzylaminopurine (BAP) concentrations (0, 1.25, 2.5, 3.75, and 5 ppm) respectively. Explants started to grow after 2 weeks of culture. Both MS salt concentration and BAP concentration treatments did not give a significant effect on the first shoot induction time, but ½ MS medium supplemented with 5 ppm BAP could induce first shoot faster than other treatments, which is within an average of 2.5 weeks after cultured (WAC). At early observation (2 to 4 WAC), medium enriched with 3.75 ppm BAP induced more shoot than the other treatments, but at the end, control medium (MS0) induced more shoot than other treatments. Starting from 2 WAC, the explants cultured on 0 ppm BAP medium grow more rapidly than the other treatments, but after 10 WAC, they grow slower. All treatments have the same trend of leaf growth. At 2 to 8 WAC, the number of leaves is rising rapidly, but at the 9 WAC, it began to slow down.
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Judul: Penentuan Nilai Eigen dan Vektor Eigen dari Matriks Tridiagonal 2-Toeplitz dengan Pendekatan Polinomial Chebyshev
Abstrak: The eigenvalues and eigenvectors of a matrix can be determined by finding its characteristic polynomials. The characteristic polynomials of a tridiagonal 2-Toeplitz matrix is shown to be closely connected to polynomials which satisfy the Chebyshev recurrence relationship. If the order of the matrix is odd, then the eigenvalues are found explicitly in terms of the Chebyshev zeros and the eigenvectors are found in terms of the polynomials satisfying the recurrence relationship. For even ordered matrices, the situation is more complicated. The problem in these cases is that although the Chebyshev recurrence formula is still applied, its initial values are not generating Chebyshev polynomials
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