Judul: Reproductive Biology of the Sumatran Rhinoceros Dicerorhinus sumatrensis (FISCHER, 1814)
Abstrak: The Sumatran rhinoceros is the most critically endangered rhinoceros species, with fewer than 300 animals left worldwide and an extremely high rate of decline (50% of wild population lost over the last decade). Very few Sumatran rhinos are to date kept in captivity only about 9 animals distributed worldwide. Moreover, although the species has been kept since the early 19th century, captive breeding program is not really successful because only three offspring have been born in captivity. The first offspring was born in 2001 in Cincinnati Zoo, USA. There are a number of reasons for this failure in captive breeding, such as a lack of understanding of the reproductive biology and there were no reliable methods for monitoring reproductive status in the Sumatran rhinoceros. Therefore, the study was focused on the development of appropriate methods for monitoring reproductive status non-invasively and to study various reproductive aspects of the species. The female Sumatran rhinoceros excreted oestrogen predominantly into urine (67.8%), whereas 14C-progesterone metabolites were almost exclusively (99.4%) eliminated via the faeces. Oestradiol-1713 glucuronide is the only abundant oestrogen in urine, whereas oestrone is the major oestrogen in faeces, with oestradiol-1713 being less abundant. Progesterone metabolites are more complex and resulted in the excretion of three major metabolites, two pregnanediols, one of which was 513- pregnane-3a, 20a-diol, and a 5-reduced 20-oxo pregnane, being identified as 513-pregnane-3a-ol-20-one. E2 and progesterone metabolites were excreted into urine within the first 24 h (time lag). In contrast, progesterone metabolites were excreted into faeces occurred within 48 hours, while oestrogen metabolites were eliminated into faeces within 72 hours. Measurement of 5-P-30H in faeces and E2 in urine can be used for monitoring oestrous cycles, determining oestrous cycle disorders and pregnancy in the Sumatran rhinoceros. 5-P-30H immunoreactive levels during the presumed luteal phase (17.3 + 6.4 jJg/g dry faeces) were on average two to three fold higher compared to the presumed follicular phase (7.8 + 3.8 jJg/g dry faeces). E2 immunoreactive concentration during follicular phase is higher 3-6 fold (28.2-335.8 ng/mg Cr, median value 96.7 ng/mg Cr) compared to E2 level during luteal phase (8.9-191.9 ng/mg Cr, median value 33.4 ng/mg Cr). Oestrous cycle length in the Sumatran rhinoceros is about 23-24 days, with the length of follicular and luteal phase about 6 days and 17 days, respectively. Pregnancy length was recorded for 475 days, and early pregnancy could be detected after day-60according to 5-P-30H level using single faecal sample. 5-P-30H arose significantly (46.3 I-lg/gr dry faeces) after day-60 above the highest 5-P-30H values during luteal phase. Since then, 5-P-30H level steady increased during pregnancy up to 10-20 fold (344.2-787.1 I-lg/gr dry faeces) higher than luteal level. Ultrasound examinations of ovaries and observation on changes in vulval appearance provide useful information on female reproductive status which can be used as a tool for monitoring the oestrous cycle when endocrine measurement is not possible. Monitoring daily changes in vulval appearance and occurrence of initial stages of sexual behaviour are useful to determine a proper timing for pairing since intensive vulval coloration and interest between the sexes usually occurs a few days
Keyword: Biology and ecology of the Sumatran rhinoceros, Biology and ecology of the Sumatran rhinoceros, Reproductive biology of the rhinoceros species, Methods of assessing reproductive status, High pressure liquid(HPLC) co-chromatography, Gas chromatography mass spectrometry, Hormon assays, Endocrine characterization

Judul: Analisis DNA Mitokomdria Badak Sumatera dalam Konservasi Genetik
Abstrak: Research on the genetics of Sumatran Rhino had been conducted at the Laboratory of Molecular Biology in March 2007 to March 2008. The aims were determining level of genetic diversity of inter-species individual, determining the phylogeny of inter-species individuals, and finding out the genetic distance of four individuals. Blood samples were taken from 2 female Sumatran rhinos (Rosa and Bina) and 2 male Sumatran rhinos (Torgamba and Andalas) from the SRS (Sumatran Rhino Sanctuary), Way Kambas National Park. Analyses was conducted using the CO I gene and D-loop mtDNA. The primers used to amplified sequence of D-Loop partial were RHDLF and RHDLR, while sequence of CO I partial were RHCOIF and RHCOIR. Data were analyzed using the MEGA (Molecular Evolutionary Genetic Analysis) program with African White rhino, African black rhino and Indian rhino from GenBank as the out-groups. The phylogeny were analyzed Neighbor-Joining (NJ) method with 1000 bootstrapped . The PCR product was 711 bp long of CO I gene area, while the D-Loop partial was 677 bp-size DNA fragments. The D-loop DNA sequence also showed that the Sumatran rhinos were significantly different with the African rhinos despite their similarity in appearance, both rhinos had two horns. The Sumatran rhinos apparently had a closer genetic distance with Indian rhinos which had single horn. This suggested that the geographical distribution more influenced by the phylogeny rather than the number of horn.
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Judul: Mencari Aproksimasi Akar-Akar Polinomial Kompleks Dengan Algoritma Weyl
Abstrak: Mencari akarMakar polinomial dengan menggunakan algoritma Weyt. Algoritma WeyJ merupakan algoritma rekursif yang proses kerjanya dapat diana10gkan dengan algoritma Bisection. Karena algoritma Weyl bergerak dalam bidang kompleks maka memerlukan nilai awal hempa bujur sangkar. Dalam proses penyempitan bujur sangkar tersebut algoritma Weyl memerlukan algorittna Turan untuk menentukan jarak antara suatu titik dengan akar polinomial yang terdekat dengan titik tersebut. Karena apabiJa jarak tersebut temyata lebih hesar dari Yl panjang diagonal bujur sangkar yang diamati maka bujur sangkar tersebut tidak memuat satu pun akar polinomial. Untuk seJanjutnya bujur sangkar tersebut diabaikan. Sebaliknya apabila jarak yang diperoleh lebih keeil dari \6 panjang diagonal bujur sangkar yang diamati, maka bujur sangkar tersebut yang akan diperkeciL
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