Judul: Pengetahuan Gizi, Keluhan Kesehatan, Kondisi Psikologis, dan Pola Pemberian Air Susu Ibu (ASI) Ibu Postpartum
Abstrak: Postpartum period is restoration period after childbirth with one of characteristics is the process of breastfeeding. This study was aimed to assess of nutritional knowledge, health complaints, psychological conditions, and their association with breastfeeding pattern on postpartum mother. A cross sectional study design and interview method was conducted in five rural areas in Dramaga Subdistrict, Bogor District, during the period of April to May 2013. Sample included in this study were 30 postpartum mothers who met inclusion criteria sample is the age of a baby 10−40 days and baby was not a first childbirth, she will be the respondent through the informed consent agreement, and be able communicate. The mayority postpartum mother had level of nutritional knowledge (60%) and health complaints (66.7%) were classified in low, and they have depression (56.7%). The nutritional knowledge of exclusive breastfeeding tends to correlate (r=0.126, p=0.508) exclusive breastfeeding practice. Meanwhile, lower health complaint tends to correlate with better breastfeeding practices.
Keyword: postpartum mother, breastfeeding, psychological, health complaints, Nutritional knowledge

Judul: Pengetahuan, Sikap, Dan Praktek Pemberian Asi Serta Status Gizi Bayi Usia 4-12 Bulan Di Perdesaan Dan Perkotaan
Abstrak: Exclusive breastfeeding rates in Indonesia is still low. Some studies indicated that low mothers' nutritional knowledge and attitude is the cause of low exclusive breastfeeding practice. The main aim of this study was to compare breastfeeding knowledge, attitude, practice and infants nutritional status between rural and urban areas. In this cross-sectional study, Desa Jayabakti, Kabupaten Sukabumi represents rural area, while Kelurahan Kedung Jaya, Kota Bogor represents urban area. Samples were 31 mothers-infants in each area who were selected by simple cluster sampling method. The differences between two areas were analyzed with the independent t-test, chi-square test, and Fisher's exact test. The correlation between variables were analyzed with rank Spearman. The result of this study showed that mother's knowledge and attitude of nutrition, especially about exclusive breastfeeding, was higher in urban than in rural area. There were differences in mothers' knowledge and attitude between rural and urban areas (p<0.05). Exclusive breastfeeding in rural area practiced by 41.9%, which is higher than in urban area (25.8%). Nevertheless, there was no statistical difference in exclusive breastfeeding practice between rural and urban areas (p>0.05). Breastfeeding practices which statistically difference in rural and urban areas were the introduction of colostrum status and brastfeeding time (p<0.05). Early initiation of breastfeeding was associated with exclusive berastfeeding practice in urban area (p<0.05), but none of the variables were associated with exclusive breastfeeding practice in rural area (p>0.05). Furthermore, exclusive breastfeeding practice was not associated with 4-12 months infants nutritional status (p>0.05). Mothers' family should be targeted as target of audience in breastfeeding promotion.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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