Judul: Ekspresi Fenotipe Padi Transgenik Pembawa Gen CsNitr1-L terhadap Variasi Pemupukan Nitrogen
Abstrak: Ammonium is the major nitrogen source in rice. Nitrate assimilation pathway can be modified by introducing CsNitr1-L gene encoding nitrite transporter to increase the rate of nitrate and nitrite transport. The more efficienct nitrogen absorbtion can increase plant productivity. The aim of this research was to determine the phenotype expression of CsNitr1-L gene of transgenic rice on various doses of nitrogen fertilization. Transgenic rice seed was selected by nitrite solution 30 mM and PCR. Transgenic plants treated with four different concentration of urea fertilizer (0, 100, 200, and 300 kg/ha), experience were done in three replicates. Result shown that there were significant corelation (P<0.05) between dosage and rice varieties to all response variables. The most efficient nitrogen absorbtion of transgenic rice was 37.84% on doses 100 kg/ha. This doses produced growth of 80.07 cm plant height, tiller number as many as 10.71 stem, panicle number as many as 16, and grain yield of 43.72 g/grove. Non-transgenic rice generate growth and good results with a dose of 200 kg/ha.
Keyword: 

Judul: Analisis Pewarisan Gen CsNitr1-L pada Tanaman Padi Ciherang Transgenik Generasi F4 dari Silang Balik Ketiga
Abstrak: Efforts to increase rice production today meet some constraints, such as lans use conversion, climate change and degradation of quality of land affecting the productivity. Farmers provide additional chemical fertilizer to increase rice productivity especially nitrogen fertilizer. Field conditions with submarging system for rice plant can result in the loss of nitrogen fertilizer due to dissolve, evaporate and undergo denitrification, so most of the amount of nitrogen fertilizer applied in rice fields can not be optimally absorbed by plants. Nitr1-L gene is a gene that encode nitrites transporter which is included in the group of proton oligopeptide transporter (POT), so the absorption of nitrites by the plants containing this transporter becomes efficient. The gene encoding this protein (CsNitr1-L) under the control of 35S CaMV promoter had been introduced into rice plants (Oriza sativa L.) subspecies Japonica cv. Nipponbare. To transfer this gene, the japonica transgenic rice had been crossed with Indica rice cv. Ciherang, followed by back-cross and self pollination until BC3F4 generation. The aim of this study was to analyse introgression of CsNitr1-L gene in the transgenic rice BC3F4 generation. The transgenic rice plants in BC3F4 generation were selected based on the resistance to hygromicin. More than 90% population of BC3F4 are putatively transgenic rice plants. These transgenic plants were confirmed by PCR analysis by using primers corresponding to hpt gene. The seed productivity of transgenic lines was higher than that of nontransgenic ones. Based on the highest productivity of every transgenic line of G3, G7, G8, G11, four transgenic plants were analysed by PCR. PCR analysis showed that these four transgenic plants contained CsNitr1-L under the control of 35S CaMV promoter. The result indicated that the transgene of CsNitr1-L was introgressed into BC3F4 generation.
Keyword: 

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
Keyword: