Judul: Analisis Faktor yang berpengaruh terhadap Keputusan Pembelian Komik Shonen Studi Kasus Mahasiswa Institut Pertanian Bogor
Abstrak: Komik shonen merupakan salah satu produk budaya popular Jepang yang diminati oleh remaja di Indonesia. Penelitian ini bertujuan untuk menganalisis karakteristik konsumen komik shonen serta menganalisis apakah faktor budaya, sosial, personal dan psikologi konsumen berpengaruh terhadap keputusan pembelian shonen manga. Penelitian ini merupakan penelitian deskriptif kuantitatif. Penelitian ini menetapkan mahasiswa Institut Pertanian Bogor sebagai populasi. Teknik pengambilan sampel dilakukan dengan metode purposive sampling. Data yang digunakan terdiri dari data primer (observasi, kuesioner) dan data sekunder (studi literatur). Teknik analisis data yang digunakan adalah analisis faktor. Hasil penelitian menunjukkan bahwa faktor- faktor baru yang terbentuk dari hasil analisis faktor berpengaruh positif terhadap keputusan pembelian shonen manga oleh konsumen. Hal ini dibuktikan dengan nilai korelasi antar faktor yang bernilai lebih besar dari 0,5.
Keyword: analisis faktor, keputusan pembelian, shonen manga

Judul: Analisis Perilaku Mahasiswa IPB dalam Proses Keputusan Pembelian Novel Berjudul “Hujan” Karya Tere Liye.
Abstrak: Rendahnya minat baca di Indonesia khususnya di Bogor, Jawa Barat menjadi ancaman bagi kualitas generasi muda. Merangsang minat baca dapat melalui bahan bacaan yang populer di kalangan anak muda seperti novel dengan tipe young adult. Sehingga, penting bagi penulis untuk mempelajari perilaku konsumen untuk dapat bersaing dengan penulis lainnya dan menciptakan karya yang berkualitas di mata pembaca. Tujuan dari penelitian ini, antara lain 1) mengidentifikasi mengenai karakteristik konsumen, 2) menganalisis proses keputusan pembelian konsumen, dan 3) menganalisis faktor-faktor yang memengaruhi perilaku kosumen dalam proses keputusan pembelian novel berjudul “Hujan” karya Tere Liye. Metode yang digunakan dalam penelitian ini adalah analisis deskriptif dan analisis faktor. Hasil analisis deskriptif menunjukkan lima tahapan dalam proses keputusan pembelian novel berjudul “Hujan” karya Tere Liye. Sedangkan, hasil analisis faktor menghasilkan empat faktor baru yang terbentuk yaitu faktor sosial, faktor atribut produk, faktor psikologis, dan faktor pribadi.
Keyword: analisis faktor, novel, perilaku konsumen, proses keputusan pembelian

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable