Judul: Analisis desain fungsional, struktural dan kondisi iklim mikro pada lumbung padi tradisional (Leuit) masyarakat baduy luar di propinsi Banten
Abstrak: Masyarakat Baduy dikenal mandiri dalam pemenuhan kebutuhan padinya. Masyarakat ini memiliki suatu lumbung padi yang disebut Leuit. Bangunan Leuit hanya memiliki satu pintu kecil untuk memasukkan dan mengeluarkan padi. Padi di dalam Leuit biasanya sangat awet dan dapat disimpan hingga lima tahun. Pada penelitian ini dilakukan analisa terhadap iklim mikro, desain fimgsional, serta desain spkhxal Leuit. Penelitian dilaksanakan di Desa Kanekes, Kecamatan Leuwidamar, Kabupaten Lebak, Banten dan Laboratorium Lingkungan dan Bangunan Pertanian, Fakultas Teknologi Pertanian IPB pada bulan April 2008 hingga September 2008. Parameter iklim mikro yang diamati antara lain suhu, RH (kelembaban relatif), dan intensitas cahaya. Parameter desain bangunan yang diamati antara lain ukuran bangunan, bahan konstruksi, berat bagian-bagian bangunan, dan jenis sambungan. Parameter tambahan yang diamati adalah proses panen dan pascapanen yang dilakukan oleh masyarakat Baduy Luar.
Keyword: 

Judul: Sistem Tenurial dan Kearifan Tradisional Masyarakat Adat Baduy dan Hubungannya dengan Sistem Pangan Lokal (Kasus Desa Kanekes, Kecamatan Leuwidamar, Kabupaten Lebak, Provinsi Banten)
Abstrak: Peningkatan jumlah penduduk mengakibatkan semakin tingginya kebutuhan terhadap lahan untuk produksi pangan. Penelitian ini dilakukan pada masyarakat Baduy Desa Kanekes, Kecamatan Leuwidamar, Kabupaten Lebak, Provinsi Banten. Tujuan dari penelitian ini adalah menganalisis sistem tenurial, kearifan lokal dalam sistem pertanian, hubungan sistem tenurial dan kearifan lokal terhadap sistem pangan lokal. Metode yang digunakan dalam penelitian ini adalah pendekatan kuantitatif didukung oleh data kualitatif dan teknik non-probability sampling dalam menentukan responden. Unit analisis dalam penelitian ini adalah rumah tangga dengan jumlah sampel sebanyak 40 responden yang dipilih secara purposive. Hasil penelitian menunjukkan bahwa sistem kepemilikan lahan pada masyarakat Baduy diperoleh melalui pewarisan dan pembelian lahan, sementara sistem penguasaan lahan diperoleh melalui sewa dan bagi hasil. Bentuk budidaya dalam pertanian masyarakat Baduy adalah tanaman campuran dengan pola tanam secara tumpang sari. Hasil panen padi secara budaya tidak boleh diperjualbelikan namun harus disimpan di dalam leuit (lumbung padi). Kearifan lokal berhubungan dengan sistem pangan lokal masyarakat Baduy. Sedangkan sistem tenurial tidak berhubungan dengan sistem pangan lokal masyarakat Baduy., The increase in population has resulted in a higher demand for land. The location of this study was in the Kanekes Village, Leuwidamar District, Lebak Regency, Banten Province. This study aims to determine tenure system, local wisdom in the agricultural system, the effect of tenure system and local wisdom on the local food This study used a quantitative with qualitative approach and used non-probability sampling techniques. The unit of analysis in this study was households with a sample of 40 respondents who were selected purposively. The results showed that the land tenure system in the Baduy community was through inheritance, buying and selling, renting, and profit-sharing. The form of cultivation in Baduy agriculture is mixed crops with an intercropping pattern. Rice yields are culturally not allowed to be traded but must be stored in leuit (rice barns). Local wisdom related to the local food system of the Baduy community. Meanwhile, the tenure system is not related to the local food system of the Baduy community. Keywords: Baduy
Keyword: kearifan tradisional, masyarakat Baduy, sistem tenurial, tenure system, traditional wisdom, Baduy community

Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
Keyword: