Judul: Analisis Variasi Genetik Populasi Tanaman Karet (Hevea brasiliensis) Sumber Eksplan untuk Perbanyakan in vitro Berdasarkan RAPD
Abstrak: Information on genetic diversity is needed to support breeding and conservation programs. Research on genetic variation in rubber plant population (Hevea brasiliensis) with RAPD (Randomly Amplified Polymorphic DNA) technique has been performed in the Biotechnology Research Institute of Plantation Indonesia (BPBPI). The observed population include 57 of 100 genotypes that was resulted from selection of 3 parental seed groups (GT 1, PB 260 and RRIM 600). This study used 7 primers, OPA 02, OPA 07, OPA 15, OPB 04, OPC 05, OPC 11 and OPC 20. The results of research showed that RAPD technique can be used to analyze the relationship between 57 genotypes of rubber plants with 3 parental seed. The most genotypes of rubber plant had closest relationship with PB 260 clone, that is similiar with genotypes showed a good response to in vitro culture. Genetic similarity coefficient values were ranged from 62-96%. The highest genetic similarity (96%) were found between genotype 70 and genotype 78.
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Judul: Analisis Keragaman Genetik Tanaman Karet Hasil Persilangan antara RRIM 600 dan PN 1546 dengan Menggunakan Teknik RAPD
Abstrak: Low productivity of rubber product had been the main problem in public rubber plantation in Indonesia. New assembly of excellent genotipe rubber plant requires 25-30 years. The more appropriate and accurate method is needed to early determine the new rubber plant clone. This research aimed to shows genetic variation of rubber plants-crossed product between RRIM 600 and PN 1546 with RAPD technique. Two DNA samples from rubber plants parent and 25 DNA samples from rubber plants-crossed products were isolated and amplified by using 16 mTcCIR combination primers and 60 RAPD combination primers. Amplification by using 16 mTcCIR combination primers produced a specific selected marker candidates, while amplification by using 60 RAPD primers produced polymorphism ribbon pattern in agarose gel. Phylogenetic tree was created based on the ribbon to identify the group and family distances of rubber plants-crossed product. Result showed that combination primer ‟d‟ and ‟i‟can be developed into specific marker candidates for RRIM 600. Phylogenetic tree showed that 25 rubber plants-crossed product have 10 high family groups with coeficient 89%. The family group showed that 25 rubber plant-crossed product had high diversity.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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