Judul: Keragaman Jenis Rayap dan Intensitas Kerusakan Bangunan di Perumahan Alam Sinarsari, Cibeureum, Darmaga, Bogor
Abstrak: Nowdays, termite attacks to buildings are very important issue with considering the intensity of their attacks which are high and extend, so that the value of losses due to termite attacks tend to increase from year to year. The demography of Kota Bogor which has high rain intensity and humidity can be one of factor causing building damages. Damage of buildings like decay and crack can be a access for termites to attack the buildings. The objective of this research is to know the species and distribution of subterranan species, along with to know damage intensity of house buildings in the location of research. Location of this research is Perumahan Alam Sinarsari with spread 280 stakes Pinus merkusii size 1.9 cm x 1.9 cm x 45.7 cm. The results showed that there found six species of subterranean termites at Perumahan Alam Sinarsari, which were Coptotermes curvignathus, Schedorhinotermes sarawakensis, S. javanicus, Odontotermes javanicus, Macrotermes gilvus, and Capritermes mohri. Meanwhile based on survey of damage and interview showed most of the houses buildings included in medium condition (61.03%) and also founded damaged caused by dry wood termite attacks.
Keyword: dry wood termite, damage of building, termite, Biodiversity

Judul: Subterranean Termite Attack on Tree Stands in Bogor Botanical Garden
Abstrak: Pelestarian dan perlindungan terhadap pohon-pohon yang tersimpan di Kebun Raya Bogor (KRB) perlu dilakukan untuk menghindari kerusakan oleh berbagai macam penyakit maupun serangga perusak termasuk rayap. Penelitian bertujuan menganalisis frekuensi serangan rayap dan mengidentifikasi jenis rayapnya, menganalisis preferensi rayap terhadap jenis pohon, serta tingkat kerusakan pohon akibat serangan rayap di KRB. Penelitian dilakukan dengan menggunakan metode jalur sampling. Hasil penelitian menunjukkan bahwa sebanyak 213 (100%) pohon contoh di KRB terserang rayap. Spesies rayap tanah yang ditemukan sekurang-kurangnya terdapat empat spesies yaitu Coptotermes curvignathus Holmgren, Macrotermes gilvus Hagen, Odontotermes javanicus Holmgren dan Nasutitermes matangensis Haviland. Rayap M. gilvus banyak ditemukan pada pohon Pterocarpus indicus, Dysoxilum spp., Shorea spp., dan Dimocarpus spp.. Rayap O. javanicus banyak ditemukan pada pohon Vitex cofassus, Dehaasia incrassata, Heriteria spp., dan Cordia spp.. Rayap N. matangensis banyak ditemukan pada pohon Philodendrom spp., Dysoxilum spp., dan Sindora spp.. Rayap C. curvignathus banyak ditemukan pada pohon Araucaria columnaris, Agathis sp., dan Pinus spp. Tingkat kerusakan pohon contoh akibat serangan rayap sebesar 10-90%., Preservation and protection of trees stored in the Bogor Botanical Gardens (KRB) need to be done to prevent damage by various kinds of diseases and destructive insects including termites. This study aimed to analyze the frequency of termite attacks and to identify the type of termites, to analyze termite preferences for tree species, as well as the degree of tree damage due to termite attacks in KRB. The research was conducted using the path sampling method. The results showed that as many as 213 (100%) sample trees in KRB were attacked by termites. There are at least four subterranean termite species found, namely Coptotermes curvignathus Holmgren, Macrotermes gilvus Hagen, Odontotermes javanicus Holmgren, and Nasutitermes matangensis Haviland. Termites M. gilvus are found in Pterocarpus indicus, Dysoxilum spp., Shorea spp., and Dimocarpus spp. Termites O. javanicus are found in Vitex colossus, Dehaasia incrassate, Heriteria spp., and Cordia spp. Termites N. matangensis is found in Philodendrom spp., Dysoxilum spp., and Sindora spp. Termites C. curvignathus can be found in Araucaria columnaris, Agathis sp., and Pinus spp. The level of damage to sample trees due to termite attacks is 10-90%.
Keyword: frequency, subterranean termites, termite preferences, tree healt

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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