Judul: Isolasi Dan Karakterisasi Enzim Mananase Bacilius pumilus DYP 2
Abstrak: Mananase adalah enzim pmgurai senyawa manan yang banyak dihasilkan oleh mikroorganisme termasuk bakteri. Enzim ini dapat dimanfilatkan pada berbagai macam proses industri dan untuk meningkatkan nilai gizi pakan temak. Penelitian ini bertujuan untuk mmgisolasi dan mmgkaraictl'2"isasi enzim mananase dari sampel tanah limbah kopra asal kabupaten Pasaman Swnatra Barat Penelitian diawali dengan mengisolasi bakte:ri dari sampeJ tanah dan menwnbuhkannya pada medium gum locust bean, lsolatisolat yang didapat diukur indeks mananolitiknya. Isolat dengan indeks mananolitik terbesar digunakan untuk membuat inokulum dan juga untuk diidentifikasi berdasarkan analisis 16S rRNA. Inokulum digWIakan Wltuk memproduksi enzim mananase. Enzim tersebut diuji aktivitasnya dengan menggunakan metode Purwadaria et al. (1994) dengan modifikasi pH 7.2. Kadar protein diukur berdasarkan metode Bradford (1976). Karakterisasi enzim dilakukan pada berbagai pH dan suhu serta dilakukan uji kestabilan aktivitas enzim. Isolasi balderi dari sampeJ tanah asal Kabupaten Pasaman Sumatra Barat menghasilkan 27 isolat dengan 4 isolat ter-baik. Isolat DYP 2 dengan indeks mananolitik terbesar yaitu 16 diidentifikasi sebagai Bacillus pumiJus. B. pumilus tersebut memmjukkan aktivitas enzim mananase optimum pada saat jam ke-24 dengan nilai 0.079 mU/mt. Pada saat aktivitas optimum didapat kadar protein sebesar 0.4 mglml dan aktivitas spesifik sebesar 0.2 mU/mg. Enzim mSllIlIlase dati B. pumilus DYP 2 memiliki suhu optimum 800c, aktivitas enzim tertinngi pada pH 3.00, dan stabil selama
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Judul: Purifikasi Parsial dan Karakterisasi Enzim Mananase dari Bacillus pumilus
Abstrak: Bacillus pumilus isolate is one of bacterial collection from Biotechnology Culture Collection (BTCC) LIPI, isolated from Pari island waters was examined to determine the ability of bacteria to degrade mannan from Locust Bean Gum (LBG). Based on qualitative analysis, this isolate showed the abilities to degrade mannan while the quantitative analysis showed the highest mannanase activity was obtained on the third day of incubation time at 3,2 U/mL with 0,9 U/mg specific activity. Initial purification using ultrafiltration 5000 WMCO showed mannanase activity at 5,1 U/mL with 1,44 U/mg specific activity. Enzyme purification by gel filtration produced a peak in fraction no.31 with mannanase activity at 4,2 U/mL with 4,00 U/mg specific activity. Analysis of hydrolitic products by thin layer chromatography showed that the main products from Locust Bean Gum are manobiose, manotetraose and manohexaose. Maximum activity of the mannanase enzyme produced by B. pumilus isolate occurred at pH 5,0 and temperature of 40°C. This enzyme has 30,17 kDa single protein band when running with SDS PAGE with Michaelis Menten (Km) and maximum velocity (Vmax) at 0,076 mg/mL and 0,02 U/mg respectively.
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Judul: Implementation of Spatio-Temporal Change Query in Relational Database.
Abstrak: Geographic object changes in various views. Object changes are recorded using a model of static storage. No historical connection between old version data and new version data has been built. For that, a storage model of spatio-temporal queries that can analyze spatio-temporal changes has been developed. The object changes the geometry are grouped according to the taxonomy of Spatio-Temporal Pattern Change. This research has been able to apply the query patterns change including born, expand, merge, contract, split, normal and die. This query based on geometric (area) changes. The implementation of spatio-temporal change queries represents an object changes continuously with easy, change detection is done by comparing two versions of data. Keyword: spatio-temporal change query, spatio-temporal change pattern.
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