Judul: Uji potensi Artemia sp. sebagai vektor pembawa vaksin DNA untuk benih ikan mas Cyprinus carpio
Abstrak: Koi Herpes Virus (KHV) is one of the most common impetuses for disease on common carp Cyprinus carpio. Generally, viral disease is difficult to cure because virus is intra-cellular parasite, that virus survives, multiplies, and lives only if it on the host cell. The DNA vaccine that gift with oral method by Artemia sp. is one alternative that expected to handle fish viral disease. This experiment was carried out to analysis DNA vaccine expression encoding of glycoprotein gene (GP11) on Cyprinus carpio. Bacterial that has contents plasmid Krt-GP11 as vaccine is served through Artemia sp as vaccine carrier. Artemia sp containing the DNA vaccine was prepared for once or twice provisions a week to three week old common carp. The existence of DNA vaccine in intestine, kidney, and gill is analyzed by PCR method. The organs were analyzed every 3 days after vaccination. The expression of GP11 in kidney organs in each treatment is also observed by the use of RT-PCR method. Expressions in the organs were analyzed 10 days after vaccination. The experiment shows that dose of DNA vaccine in whole bacteria could be expressed is 106 cfu in a once or twice provisions a week. DNA vaccine could be detected in three organs. Also, RT-PCR analysis shows that the expression of GP11 can be detected in all tested organs. In conclusion, Artemia sp. can be employed as a vector to carry for plasmid GP11 vaccine with 90 minutes submersion.
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Judul: Persistensi vaksin DNA penyandi glikoprotein 25 yang diberikan melalui pakan buatan pada ikan mas (Cyprinus carpio)
Abstrak: Koi Herpes Virus (KHV) is a major viral pathogen that infects common carp and koi. KHV disease outbreak in almost all centre of common carp culture in Indonesia and caused mass mortality. DNA vaccination method is one of ways to cope with KHV infection. Vaccines were commonly given by injection. In this research, persistence test of DNA vaccine (pMBa-GP25) were carried out by oral feeding on common carp. DNA vaccine persistence test were done using PCR method with the specific primer for GP25 gene. Escherichia coli DH5α bacteria containing pMBa-GP25 DNA vaccine were cultured in four treatment mediums namely 2xYT, LB polypeptone, LB triptone and TSB. Bacteria densities were counted with spectrophotometer using 580 nm wave lengths. The results showed that an efficient culture media for E. coli was LB tripton. Furthermore, GP25 DNA was detected in kidney and spleen of vaccinated fish. Level of DNA vaccine that could be detected was 7,56 ng (amount of DNA copy 1,598 x 1010 copies).
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Judul: Pembentukan tanah berbahan induk andesit-basaltik dan satuan-satuan tanah menurut sistem soil taxonomy pada suatu toposekuens di daerah Ujung Berung Bandung
Abstrak: Tujuan penelitian adalah untuk melihat satuan-satuan tanah pada toposekuens lereng G.Manglayang, Ujungberung, Bandung menurut sistem Soil Taxonomy dalam hubungannya dengan pembentukan dan perkembangan tanah yang didasarkan pada sifat fisik, kimia, dan kandungan mineralnya sebagai hasil dari proses penambahan, pengurangan, pemindahan, dan pengubahan dalam sistem di dalam profil tanah. Pada daerah perbukitan, faktor topografi sangat berperanan dalam proses pembentukan dan perkembangan tanah. Faktor topografi mempengaruhi pergerakan air baik secara vertikal maupun horisontal, sehingga berpengaruh terhadap tingkat kelembaban, proses pelapukan, pencucian dan pemiskinan serta proses erosi dan deposisi. Proses-proses ini akan menentukan sifat-sifat tanah secara fisik, kimia maupun kandungan mineralnya. Topografi semakin cembung menunjukkan tingkatan umur pembentukan tanah yang lebih lanjut dan cenderung membentuk tanah-tanah bersifat oksik dengan kandungan mineral liat tipe 1:1, yaitu Kaolinit, sehingga tingkat kesuburannya lebih rendah. Sedangkan pada topografi cekung cenderung terbentuk tanah muda, yaitu tanah belum berkembang dan mulai berkembang dengan kandungan mineral amorf. Pada topografi rektilinier cenderung terbentuk tanah berkembang dengan pembentukan horison Argilik.
Keyword: Sifat fisik, Sifat kimia