Judul: Pengaruh waktu penyimpanan terhadap kemampuan deteksi antibodi poliklonal (IgY) anti Ekskretori-Sekretori (ES) Fasciola gigantica pada uji ELISA
Abstrak: IgY merupakan antibodi utama pada ayam dan memiliki struktur yang homolog. IgY stabil terhadap suhu panas dan denaturasi guanidin-HCl dibandingkan IgG kelinci tetapi konformasi molekul IgY akan mengalami perubahan dalam suasana asam yang menghasilkan penurunan aktivitas antibodi. Tujuan penelitian ini adalah mengetahui pengaruh waktu penyimpanan terhadap kemampuan deteksi antibodi poliklonal (IgY) anti ekskretori-sekretori (ES) Fasciola gigantica pada uji ELISA. Dua sampel IgY yang diproduksi pada tahun 2009 dan 2011 digunakan dalam penelitian ini. IgY murni tersebut disimpan pada suhu - 20 oC selama 2 tahun dan 4 tahun. Konsentrasi IgY diukur sebelum dan sesudah penyimpanan menggunakan EGGstract® IgY Purification System (PROMEGA). Aktivitas IgY diukur dengan mengukur titer antigen ES F. gigantica menggunakan uji ELISA tidak langsung. Hasil menunjukkan bahwa konsentrasi IgY tahun 2009 menurun sebesar 19.95%, sedangkan konsentrasi IgY tahun 2011 menurun 41.82%. Titer IgY anti ES tahun 2009 juga lebih tinggi dibandingkan titer IgY tahun 2011. Masing-masing IgY masih aktif pada tingkat pengenceran 200. Hasil ini dapat disimpulkan bahwa aktivitas IgY tidak dipengaruhi oleh waktu penyimpanan tetapi kualitas IgY pada saat diproduksi pada telur.
Keyword: Bogor Agricultural University, Institut Pertanian Bogor, IPB, Excretory-Secretory F. gigantica, IgY, Storage time

Judul: Optimasi Penggunaan IgY Dan IgG Sebagai Antibodi Penangkap Dan Pendeteksi Antigen Ekskretori Sekretori (ES) Fasciola gigantica Dalam Uji Elisa
Abstrak: Fasciolosis is a parasitic disease of domestic ruminant that cause high economic loss. Currently diagnosis of fasciolosis relies on microscopic examination of Fasciola sp. eggs in fecal sample. Serological test such as ELISA has been developed to improve the diagnostic method of fasciolosis. This study was designed to optimize the use of polyclonal antibodies (IgY and IgG) as capture antibody and detection antibody in ELISA assay to detect excretory secretory (ES) of Fasciola gigantica. The conformation was done in two models which compared IgY (9,57 mg/ml) and IgG (3,75 mg/ml) as capture antibody. The first conformation was IgY as capture antibody in 1:1000 dilution and IgG as detection antibody in 1:100, 1:1000, and 1:10000 dilutions. The second conformation was IgG as capture antibody in 1:1000 dilution and IgY as detection antibody in 1:100, 1:1000, and 1:10000 dilutions. The results showed that IgY as capture antibody in 1:1000 dilution and IgG as detection antibody in 1:100, 1:1000, 1:10000 dilutions were able to capture ES antigen 3,87; 2,85; and 1,65 times than cut off value. The other conformation with IgG as capture antibody in 1:1000 dilution was able to capture ES antigen as much as 1,91 times than cut off value with IgY as detection antibody in 1:100 dilution. This showed that the good optimation ELISA was IgY as capture antibody in 1:1000 dilution and IgG as detection antibody in 1:100 dilution.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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