Judul: Penentuan Waktu Optimal Pengujian Integritas Membran Plasma Spermatozoa Babi Mengunakan Hypo-osmotic Swelling (HOS) Test
Abstrak: Keutuhan membran plasma merupakan faktor penting untuk menentukan fertilitas spermatozoa. Penelitian ini bertujuan untuk menentukan waktu optimum untuk pengujian membran plasma yang utuh (MPU) pada semen segar babi menggunakan hypo-osmotic swelling (HOS) test. Sebanyak 10 ekor babi dari bangsa Landrace, Duroc dan Yorkshire yang telah dewasa digunakan sebagai sumber semen. Semen dikoleksi menggunakan pemijatan massase. Semen yang diperoleh dievaluasi secara makroskopis dan mikroskopis. Pengujian MPU dilakukan dengan cara memasukkan 50 μL semen ke dalam mikrotub berisi 1 ml larutan hipoosmotik (150 mOsm Kg-1). Campuran larutan diinkubasi pada suhu 37oC. Sperma yang bereaksi dan yang tidak bereaksi terhadap larutan HOS dievaluasi mulai jam ke 0 dan setiap 15 menit dengan total 200 sel sperma. Hasil penelitian menunjukkan bahwa waktu optimal sperma bereaksi maksimal terhadap larutan HOS adalah 60 menit setelah inkubasi.
Keyword: Keutuhan membran plasma, HOS test, semen babi.

Judul: Penentuan Waktu Optimal Pengujian Keutuhan Membran Plasma Spermatozoa Semen Beku Sapi Limousin dan Frisien Holstein Menggunakan Hypo-Osmotic Swelling (HOS) Test.
Abstrak: The study was conducted to determine the optimal time to test the integrity of plasma membrane of Limousin and Frisien Holstein (FH) bull frozen semen spermpatozoa using hypoosmotic swelling (HOS) test. A total of 15 samples from each bull used. All samples were incubated in a hypoosmotic solution with osmolarity of 150 mOsm Kg-1 for 60 minutes. The hypoosmotic solution made by admix 1.351 g fructose, 0.735 Na-citrate and water until the volume was 100 ml. Every 15 minutes the whole sample was evaluated the percentage of spermatozoa that reacts to the solution. Optimal time was obtained at 30’ and 45’, both on Limousin and FH frozen semen. The mean percentage of reacted Limousin bull spermatozoa at 30’ and 45’ respectively were 53.90 ± 6.81 % and 52.47 ± 8.00 %. Whereas the FH bull spermatozoa was 44.52 ± 6.26 % and 44.46 ± 8.42 %. Spermatozoa from frozoen semen needs time to react to hypoosmotic solution due to the added of freezing diluent. This causes the spermaozoa did not react immediately to the hypoosmotic solution. Plasma membrane integrity is very important for sperm fertility. Fertility enzymes contained in the head of spermatozoa, thus if the plasma membrane of the head broken, spermatozoa will lose their fertility. Similarly in the tail, if the plasma membrane broken, spermatozoa will lose their motility.
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Judul: Kajian Konsep Desain Lanskap Jalan Kolonial Kota Bogor.
Abstrak: Bogor City is one of the historic sites in the European colonial era in Indonesia. One of a heritage form in that era is the landscape of colonial. The form of colonial landscape also reflected in the design of street landscape in Bogor City. Study on Design Concept of Colonial Streetscape at Bogor City aims to identify the component of street landscape whose colonial character, exploring the design concept of colonial street landscape, and composing the design concept of street landscape that having colonial character in Bogor. This research has obtained through descriptive analysis by field observation and tracing the information of history. Generally, three main components of colonial street landscape design those are the main elements of street, complementary elements, and colonial building. Complementary elements that characterizing the colonial has the feel of a classic Victorian, gothic and use of industrial materials. The third component of colonial street lanscape is a building that reflects the Dutch colonial era and The important part of it is the facade of the building.
Keyword: colonial concept, street landscape design