Judul: Characterization of Protease from Blood and Cloning of Factor C cDNA from Indonesian Horseshoe Crab (Tachypleus gigas)
Abstrak: The horseshoe crab blood has some medical benefits, especially for endotoxin detection, which is an ability thought to be due to proteases’ role. Therefore, this study aimed to characterize proteases derived and to isolate factor C from horseshoe crab blood collected from Banyuasin Estuary, South Sumatra, Indonesia. With the blood sample, 1 EU/ mL of endotoxin was detected using the LAL test method, but a concentration of 0.5 EU/mL was not detected. Furthermore, the molecular mass was between 60-75 kDa and 25-35 kDa. It also had protease activity at a molecular mass below 60 kDa, while the highest activity was at temperatures 70 °C as well as pH 11. Besides, the enzyme’s activity was mostly inhibited in the presence of PMSF, EDTA, and SDS, but potentially increased by Tween-20 and cofactors such as Mg2+ and Ca2+. Based on the results, the enzymes contained in horseshoe crab blood belong to serine proteases. Light chain factor C fragment has been isolated from the blood of Tachypleus gigas (990 bp) and ligated to pGem-T-easy. The light chain sequence from T.gigas is similar to T. tridentatus sequence with a percent identity of 98.38%.
Keyword: blood, endotoxin, factor C, horseshoe crab, serine protease

Judul: Karakterisasi aktivitas dialisat enzim protease fibrinolitik dari cacing tanah (Eisenia foetida) galur lokal
Abstrak: Commercially available thrombolytic drugs were usually administered intraveously and priced above consumer resources. Earthworm, in several Eastern Asia countries, was known for its thrombolytic properties. Its herbal drug had been used to increase blood flow and to treat leakage in blood vessel. Thrombolytical properties of fibrinolytic protease (lumbrokinase) enzyme from local strain Eisenia foetida had never been reported. This research was purposed to characterize its enzyme activity and its specificity to fibrin as substrate. Enzyme was purified through precipitation, dialysis, and column chromatography fractionation. The highest protease activity was obtained when the dialysate sample was incubated for 10 minutes in pH 8 at 60°C which activity was 0.239 Um . Spectrophotometry based analysis showed that lumbrokinase was able to degrade fibrin up to 30.9% compared to sample without enzyme addition. Working condition to obtain fibrinolytic activity was 10 minutes incubation in pH 8 at 60°C. Lumbrokinase samples electrophoresis at each step have molecular weight between 25.200 and 29.318 kD. The highest protein concentration was found in dialysate with 1.74 m m . Obtained dialysate has protein purity up to 72.28% higher than crude extract.
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Judul: Uji Konsolidasi Pada Tanah Podsolik dan Regosol
Abstrak: Disain rangka bangunan atau struktur lainnya pada prinsipnya didasarkan pada asumsi bahwa struktur berdiri di atas lapisan dasar yang tidak lembek. Pada kenyatannya, berat setiap struktur memampatkan dan mendeformasikan lapisan tanah di bawahnya, dan sebagai konsekuensinya asumsi disain sulit untuk dipenuhi dengan tepat. Jika lapisan dasar suatu struktur tetap datar, maka penurunan tidak rnenjadi persoalan karena tegangan dalam kerangka bangunan tidak berubah. Dilain pihak, jika berat bangunan yang mengakibatkan melengkungnya daeah yang dibebani, maka dasar srtuktur tersebut juga akan melengkung dan keseluruhan kerangka bangunan akan berubah. Kondisi di atas merupakan ha1 yang cukup penting dalam perusakan wujud bangunan atau merupakan penyebab kerusakan permanen dan kerusakan yang tidak dapat diperbaiki.
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