Judul: Konstruksi dan Analisis Kualitas Pustaka Genom Kedelai (Glycine max (L.) Merr.) untuk Sekuensing Genom Total
Abstrak: Soybean is one of the most important agricultural commodities in the supply of food in Indonesia. However, the low of the national soybean productivity is a constraint in soybean cultivation in Indonesia. National soybean productivity can be increased by genetic improvement of plants. Whole genome sequencing and genomic analysis of soybean is one way to accelerate soybean breeding progress and improve understanding of genetic and genomic information of soybean. Purposes of this research were to construct genomic library of three Indonesian soybean genotypes (Tambora, B3293 and Grobogan) and analyze the quality of the genomic library on whole genome sequencing result. Construction of genomic libraries was carried out in vitro by attaching an adapter to the end of DNA fragments. Clasterization and sequencing of genomic library in this research were conducted by using cBOT claster generation dan Next Generation Sequencing HiSeq2000. Genomic libraries were constructed successfully sized 400 bp with a concentration of 23.20 ng/μL (Tambora), 64.50 ng/μL (B3293), and 21.20 ng/μL (Grobogan). Size and concentration of the constructed genomic libraries were suitable for whole genome sequencing. The total yield of bases generated from the sequencing was 50.1 x 109 bp. Cluster library produced during the process of genome sequencing was Q scores value above 30 was 88.6%. Error rate of base reading during the sequencing was 0.97%. Values of the cluster density, percent cluster PF, the intensity of the base, phasing percent, and prephasing percent generated from this research showed that the cluster quality of genomic libraries was of ideal category.
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Judul: Konstruksi dan Analisis Kualitas Pustaka Genom Jagung (Zea mays (L.)) untuk Sekuensing Genom Total
Abstrak: Whole genome sequencing and analysis of the maize genome is one of the ways to accelerate the increase in corn breeding and to improve the understanding of the genetic and genomic information maize. The purpose of this study is to construct genomic libraries of four maize genotypes in Indonesia (Sp010BB-3-2- 2-1-B, LK2-45, PSG59-8 (early maturing) and LBP54-50 (pulut)) and analyze the quality of the results of genomic libraries total genome sequencing. Genomic library construction performed in vitro by attaching adapters to the ends of DNA fragments. Clasterization and sequencing of genomic library in this research were conducted by using cBOT claster generation and Next Generation Sequencing HiSeq2000. The amount of base produced during the sequencing process as many as 287.1 Gbp. Cluster genomic library generated with Q values above 30 scores ranged from 80.8 – 87.9%. Base reading error rate during the sequencing process was 0.32 – 0.51%. Over all sequencing results showed that quality of the genomic library cluster was belong to the ideal category.
Keyword: Bogor Agricultural University (IPB), Next Generation Sequencing (NGS)., Whole Genome Sequencing, Genomic Library, Maize (Zea mays (L.))

Judul: Infeksi Virus Parainfluenza Pada Anjing
Abstrak: Virus parainfluenza anjing termasuk famili Paramyxoviridae, berbentuk pleomorf dengan ukuran bervariasi (bergaris tengah antara 100-500 nm), berpembungkus peka ether, berisi genom RNA dan secara serologis mempunyai kesamaan dengan virus SV-5 kera; dikenak sebagai salah satu kausa penting dari sindrom "kennel cough". Infeksinya dapat mengenai anjing semua umur yang belum pernah terinfeksi atau mendapatkan vaksinasi mengakibatkan gejala klinis ringan yang terbatas pada traktus respirasi atu infeksi yang secara klinis tidak memperlihatkan gejala nyata. Gejala klinis yang nampak setelah masa inkubasi (1-5 hari) terlewati berupa batuk yang tidak berat selama beberapa hari, berbagai tingkat tonsilitis dan pharyngitis dan kadang-kadang ditemukan kenaikan temperatur rektal antara 1-2 derajat C dari temperatur normal rat-rata. Anjing terinfeksi mengandung virus pada traktus respirasi bagian atas dan bawah serta limfonoduli sampai hari ke 9 setelah infeksi. Penyebaran virus antar anjing terjadi secara aerogen.
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