Judul: Studi Keanekaragaman dan Penyebaran Spasial Palem-Paleman (Arecaceae) di Hutan Lindung Gunung Slamet, Baturaden – Provinsi Jawa Tengah
Abstrak: Palm is one of interesting plant. It has been used for several needs and also a trading commodity. One of palms habitat is in Protected Forest of Mount Slamet. The threat of land conversion and forest fires is a problem in this area. The lack of information on the diversity, abundance and the distribution of palms make this area vulnerable and increated the possibility of unknown palms to go extinct. The purpose of the research is to describe the composition, diversity and the ecological distribution of palms and also analyzing the distribution of palms based on land and altitude. Research was conducted on May until June 2012, on two type of ecosystems natural forest and mix forest at Protected Forests of Mount Slamet. Data were collected using vegetation analysis method. Data of the palms were analyzed to obtain value of diversity, evenness and abundance of species. Spatial distribution Data were obtained from recording palms position. Thematic map of land and altitude was developed on processing the landsat map and divided them to different classes. Then, it was overlayed with palms point of occurence to produce a map of palms distribution based on land and altitude. Composition of palms on Protected Forests of Mount Slamet, on natural forest ecosystem found 12 species, and on mix forest ecosystem found 6 species. Distribution pattern of palms is clumped. The higest diversity of palms occurs on natural forest at 1.200 mdpl-1.300 mdpl with elevation 8-15 %, and mix forest at 900-1.000 mdpl with elevation 8-15%.
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Judul: Keanekaragaman Tumbuhan Berguna di Hutan Lindung Gunung Slamet RPH Baturraden, BKPH Gunung Slamet Barat, KPH Banyumas Timur
Abstrak: Protected forest was a forest that had main function as a protective life support system, to managed hydrology system, prevent flooding and erosion, reduce sea water intrusion, and maintained soil fertility. This study aimed to determined the diversity of usefulness plants in protected forests of Slamet Mount RPH Baturraden, BKPH Gunung Slamet Barat, KPH Banyumas Timur. The results of the analysis of vegetation found as many as 130 plant species belonging to 63 families. The most families that found were Euphorbiaceae and habitus were mostly the tree. There were 97% of plants that have the potential for useful plants or as many as 126 species. Potential uses include as food-producing plants, medicinal plants, plants producing construction materials, plants producing rope, webbing, and handicrafts, ornamental plants, plants producing animal feed, plants producer of color, wood-producing plants, plants producing beverages, pesticide-producing plant, plants for traditional rituals and religious purposes, and producing aromatic plants, as well as other uses.
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Judul: Isolasi dan purifikasi inhibitor RNA helikase virus hepatitis C dari bakteriosin bakteri asam laktat S34
Abstrak: Target molecular therapy is one of method to prevent hepatitis C virus which was developed recently. NS3 Helicase/RNA helicase hepatitis C virus inhibition using inhibitory peptide are target in molecular therapy. Bacteriocin are inhibitory peptides which can inhibit hepatitis C virus through inhibition of NS3 helicase. Bacteriocin is a peptide which is produced by lactic acid bacteria. The aim of this research were to isolate and to purify bacteriocin from S34 lactic acid bacteria which has high activity to inhibit RNA helicase of hepatitis C virus. S34 lactic acid bacteria were identified using 16S RNA amplification and DNA sequencing identification of S34 lactic acid bacteria was Lactobacillus plantarum WCFS1. The bacterial growth reached logaritmic phase at 7 hours and stationary phase at 10 hours. Bacteriocin were isolated and purified by ammonium sulphate precipitation with 80% saturation, gel filtration chromatography, and characterization of bacteriocin through molecular weight analysis and measured protein concentration. Bacteriocin from S34 lactic acid bacteria were included in 2nd class bacteriocin, had 64.20 % of inhibition against RNA helicase hepatitis C virus, had molecular weight about 1.7-4.2 kDa, and specific activity in the amount of 2.84 x 106 U/mg.
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