Judul: ultur In Vitro Sel-sel Otak Besar (Cerebrum) Anak Tikus
Abstrak: Research has been conducted on in vitro culture of three days old rat cerebrum cells in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% Fetal Bovine Serum (FBS) and 50 μg/ml gentamycin, and supplemented with and without 5 μg/ml insulin, 10 μg/ml transferin, 5 μg/ml selenium (ITS). Culture was done in 5% CO2 incubator at 37oC for 11 days. Identification was done on types of cell based on morphological observation, growth capacity (cell proliferation, population doubling time/ PDT and length of axon and dendrit) based on calculation of the number of cells using hemocytometer and micrometer, and the protein produced by the cerebrum cells in the culture medium using sodium dodecyl sulfate–polyacrilamide gel electrophoresis (SDS-PAGE) method. Quantitative data were analyzed using statistical T-test on Minitab program. The results showed that based on the morphological observations, there are two types of cells including nerve cells of bipolar and multipolar neurons and glial cells including the fibrous astrocyte, protoplasmic astrocyte, oligodendrocyte, and microglia. Supplementation of ITS into the culture medium could increase the cells proliferation rate (P<0.05) with lower PDT, axon and dendrit lenght growth and quantitatively the 21.5–36.5 kDa protein production as indicated by the intensity of the protein band.
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Judul: Kultur In Vitro Sel-sel Fibroblas Fetus Tikus
Abstrak: Research has been conducted on the in vitro culture of rat fetal fibroblast using Dulbeccos’ Modified Eagle Medium supplemented with 3mM sodium bicarbonate, 10% fetal calf serum and 50 μg/ml gentamycin (mDMEM) in CO2 incubator at 37oC. Evaluation was done on the proliferation rate and the protein production after several passages. The growth of the fibroblast was confirmed by their morphology. The proliferation rate was done based on the population doubling time (PDT) by counting the number of cells in each passage by using Improved Neubauer hemocytometer. Identification of proteins was done quatitatively using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Based on morphological observation the cell grown in in vitro culture were the fibrocyte and fibroblast and showed increasing of homogenicity after several passages. Based on the PDT, the proliferation rate was increased by each passages. Fibroblast also secrete proteins remain until passage 9 based on the results of electrophoresis. It can be concluded that the growth and protein secretion of rat fetal fibroblast in in vitro culture can be maintained up to 9 passages.
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Judul: Distribusi Massa Air Laut Jawa dari Data Hidrografi MAJAFLOX Cruise 2015.
Abstrak: Laut Jawa merupakan perairan dangkal yang dipengaruhi oleh Angin Muson dan Arlindo Selat Makassar. Pemahaman tentang stratifikasi dan distribusi massa air Laut Jawa dapat dilakukan dengan adanya data hidrografi dari MAJAFLOX Cruise pada bulan Agustus 2015. Data hidrografi didapatkan dari penurunan CTD di 14 stasiun pengamatan. Hasil penelitan menunjukkan bahwa stratifikasi vertikal massa air dicirikan oleh lapisan homogen yang kuat dari permukaan hingga dasar perairan. Hal tersebut terjadi akibat percampuran massa air yang kuat oleh aksi gelombang yang dibangkitkan Angin Muson Tenggara. Selain itu, ditemukan variasi spasial dalam arah utara-selatan, dimana karakteristik massa air di tepi selatan dan utara cukup berbeda dengan massa air di tengahnya. Massa air Laut Jawa pada puncak musim timur (Agustus) dicirikan dengan massa air yang hangat (27,18 °C – 28,11 °C), bersalinitas rendah (33,58 psu – 34,2 psu), dan ringan (21,33 kg/m3 – 22,06 kg/m3), kecuali pada stasiun 1 dan stasiun 5 yang cenderung lebih hangat dan lebih tawar dari stasiun lainnya. Pola sirkulasi permukaan menunjukkan salinitas yang relatif tinggi di bagian tengah Laut Jawa dan diduga berhubungan dengan suplai massa air Arlindo Selat Makassar. Variasi tahunan dari analisis keluaran model INDESO menunjukkan adanya stratifikasi massa air yang kuat pada musim peralihan.
Keyword: angin muson, keluaran model INDESO, MAJAFLOX Cruise 2015, pengukuran CTD, stratifikasi massa air