Judul: The Promotore and Intron Nucleotide Variation of Myostatin Gene within Indonesiau Local Sheeps
Abstrak: lvIyostatin or growth and di/Terentiation Jactor 8 (GOF-8) is a member of transforming growth Jactor-il (TG f.-il) superfamily of growth and differentiation factors. GOF-8 is essential for proper regulation of skeletal muscle mass in vertebrate, that is expressed specifically postnatal. In mutations condition, it is suggested that gene to be a "loss of function" which give an effect to increasing skeletal mass, due to the increasing number of muscle tlbres (hypelplasia) or the size of muscle fibres (hypertrophy). Some mutation that occurs not in a loss of function, might be improve their ability to promote muscle growth or even in decreasing muscle size. The PCRSSCP technique was used to identify of nucleotide mutation in promotor region and intron 2 of myostatin gene of some local sheep in Indonesia. A total of 145 blood samples were used in this study comprised four localities, i.e. Cibanteng (43), Cilebut (26), UP3 Jonggol (38) and Wanaraja (38). The result showed that there are four types of myostatin gene based on T'CR-SSCP (type K, L, lvI and N) promoter region. The K and lvI types were found in all population, however the Land N types were not found in Wanaraja and Cilebut population respectivly. In the region of intron 2 we found five defer<;nt types of gene. There are gene type of A, E, C, F and G. The A type gene from intron 2 found only in Cibanteng population. From sequencing data, K, lvi, and N gen type were found from the promotor area. There are 8 mutation points in gen type K, 4 mutation points in gen type lvi, and 34 mutation points in gen type N. However, the gene types did not correlate whith the growth rate of the sheep. Nevertheless, the mutated gene described the polymorphism of Indonesian local sheep.
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Judul: Identifikasi Keragaman Gen Miostatin pada Domba Lokal
Abstrak: Myostatin is a member of tranforming grown factor B superfamily, know as growth and differentiation factor, which is essensial for proper regulation of skeletal muscle mass in wide range vertebrate species. To pursue potential agricultural application of increasing muscle mass by inhibition of myostatin activities, the aim of this study was to identify the variants of myostatin gene in the Indonesian local sheeps. A total of 151 DNA samples belonging to eight different areas that are Bogor Ciomas (20), Bogor Jonggol (35), Margawati (18), Indramayu (30), Dinggala (24), MAdura (8), Sumbawa (8), and Rote (8) were analyzed with Polymerase Chain Reaction-Single Starnd Conformation Polymorphism. The Myostatin (GDF 8) amplified product was 688 bp(base pair)
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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