Judul: Analisis Pola Aktivitas, Tingkat Kelelahan dan Status Anemia  serta Pengaruhnya terhadap Prestasi Belajar Siswa
Abstrak: Penyelenggaraan pendidikan di Indonesia pada umumnya bersifat klasikal, kelemahan sistem ini tidak terakomodasikannya kebutuhan individual siswa yang pada dasarnya tidak sama baik intelegensi, bakat dan minatnya. Penyelenggaraan kelas akselerasi (percepatan belajar) dianggap salah satu alternatif bagi siswa yang memiliki kemampuan dan kecerdasan di atas rata-rata. Namun, penyelenggaraan kelas akselerasi yang sudah diujicobakan beberapa tahun terakhir ini masih mengandung pro dan kontra dari banyak kalangan. Terkait dengan hal tersebut peneliti tertarik untuk meneliti bagaimana keragaan pola aktivitas, tingkat kelelahan dan status anemia serta pengaruhnya terhadap prestasi belajar siswa.
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Judul: Determinan status anemia, aktivitas fisik dan prestasi belajar siswa di SDN Pasanggrahan II Purwakarta
Abstrak: This study was aimed to examine determinant of anemia status, physical activity and academic achievement of students. This research used a cross sectional study. The number of samples were 52 elementary school student. Food consumption recall was used to measure iron source food consumption, academic achievement data was taken from exam semester and linear regression used for analysis determinants. Result showed a large number of children in normal status (51.92%) while the rest was anemia (48.08%). Nutritional knowledge of anemia student and normal student was low. Both anemia (68%) and normal student (55.6%) experience severe energy deficit, physical activity of anemia and normal students was classified as light. Academic achievement of students were very low with the score only below 60. Spearman correlation showed there was no relationship between physical activity with anemia status (p>0.05) and Pearson’s correlation showed there’s no relationship between academic score with anemia status (p>0.05). The result of regression analysis showed that poultry has negative significant effect anemia status.
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Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable