Judul: Fenotipe domba lokal di unit pendidikan penelitian dan peternakan Jonggol
Abstrak: This research was a comparative study of qualitative and quantitative characters of local sheep at UP3J (Jonggol Animal Science Teaching and Research Unit) in 1992 and 2006. The research was conducted from March up to the end of April 2006. The number of observation sheep were 242 heads. It was grouped based on sex and age. Analyzed of body dimensions were description and regression analysized. The percentage of body color patterns were calculated.
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Judul: Karakteristik Pertumbuhan Pra dan Pascasapih Domba Lokal di Unit Pendidikan dan Penelitian Peternakan Jonggol Institut Pertanian Bogor (UP3J-IPB)
Abstrak: Selection program on sheep needs some important factors like season, sex, body weight and age of dam. This research was aimed to get the characteristic of pre and post-weaning growth of local sheep in Jonggol Animal Science Teaching and Research Unit (JASTRU) IPB and selection criteria. The result can be used as a basic information on selection program to grade up the genetic properties of these sheep. This research located at JASTRU IPB, Singasari Village, sub district of Jonggol from June 2007 to May 2008 which represent two different seasons, rainy and dry. The number of sheep in the beginning of research was 154 head and in the end of research was only 33 head. The result showed that the value of Temperature Humidity Index were on slight and adequate heat. Statistic t test showed that there was a significant difference (p<0,05) between male and female birth weight in rainy season but in age of 90 and 180 day showed no significant difference. Also, birth weight between rainy and dry season was significantly different (P<0,05) and body weight on age 90 and 180 day was not significantly different. Variance analysis showed that age of dam significantly affected the male birth weight in dry season (p<0,05) and female birth weight in rainy season (p<0,05). The highest lamb body weight was owned by lamb from 2,5 to 3,0 years of dam. Pre-weaning weekly average daily gain (ADG) was higher than that of post-weaning, both on male and female, and rainy and dry season. Body weight on age 90 day can be used as a selection criteria and selection on dam can be done at age 1 to 2 years of age. Selection response of 90 day weight in dry season was higher than that of rainy season.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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