Judul: Gaya Pengasuhan Ibu, Interaksi Saudara Kandung, dan Kemandirian pada Remaja Anak Sulung Perempuan
Abstrak: Mencapai kemandirian merupakan salah satu tugas perkembangan yang harus dilakukan oleh remaja. Penelitian ini bertujuan untuk menganalisis hubungan gaya pengasuhan ibu (Parental Acceptance-Rejection), interaksi saudara kandung dan kemandirian pada remaja anak sulung perempuan. Penarikan contoh dilakukan secara purposive pada 60 remaja perempuan yang berusia 13-16 tahun di SMPN 2 Dramaga, Bogor. Contoh dalam penelitian ini dibedakan berdasarkan jenis kelamin adik yaitu remaja yang memiliki adik laki-laki sebanyak 30 orang dan adik perempuan sebanyak 30 orang. Hasil menunjukkan bahwa remaja mempersepsikan ibu melakukan pengasuhan penerimaan, interaksi kekuasaan saudara kandung memiliki indeks lebih tinggi daripada dimensi lain, dan kemandirian nilai memiliki indeks tinggi. Semakin tingginya penerimaan ibu akan meningkatkan kehangatan saudara kandung dan tingginya pengabaian ibu akan menurunkan kemandirian emosional pada remaja dengan adik perempuan. Pada remaja dengan adik laki-laki, penerimaan ibu akan meningkatkan persaingan dan interaksi kehangatan saudara kandung akan meningkatkan kemandirian emosional.
Keyword: anak sulung, gaya pengasuhan ibu (Parental Acceptence-Rejection), interaksi saudara kandung, kemandirian, remaja perempuan

Judul: Gaya Pengasuhan Penerimaan Penolakan Ibu, Interaksi Saudara Kandung, dan Self-Esteem Pada Remaja Perempuan, Gaya Pengasuhan Penerimaan Penolakan Ibu, Interaksi Saudara Kandung, dan Self-Esteem Pada Remaja Perempuan
Abstrak: Tujuan penelitian ini adalah untuk menganalisis pengaruh dari gaya pengasuhan ibu, interaksi saudara kandung, dan self-esteem remaja perempuan anak kedua. Jumlah contoh penelitian ini adalah 60 orang remaja perempuan (30 remaja dengan kakak perempuan dan 30 remaja dengan kakak laki-laki). Penelitian ini merupakan penelitian cross-sectional study dengan teknik sampling purposif. Hasil penelitian ini menunjukkan interaksi saudara kandung kehangatan remaja dengan kakak perempuan lebih tinggi daripada remaja dengan kakak lakilaki. Gaya pengasuhan penerimaan memiliki hubungan positif dengan usia dan lama pendidikan ibu. Gaya pengasuhan penolakan memiliki hubungan positif dengan dengan interaksi saudara kandung kekuatan relatif. Gaya pengasuhan agresivitas dan penolakan memiliki hubungan positif dengan interaksi saudara kandung konflik. Gaya pengasuhan agresivitas, pengabaian, dan penolakan memiliki hubungan negatif dengan interaksi saudara kandung persaingan. Terdapat pengaruh postif gaya pengasuhan kehangatan terhadap self-esteem. Selain itu, terdapat pengaruh negatif gaya pengasuhan penolakan terhadap selfesteem.
Keyword: gaya pengasuhan ibu, interaksi saudara kandung, self-esteem, anak perempuan, anak kedua

Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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