Judul: Using cytochrome β (Cyt β) gene for species identification of dog, cat, and tiger to ensure food authenticity and drugs
Abstrak: Adulteration food products from animal still happen until now. Species identification method development in food and medicine derived from animal was needed to prevent falsification information. Molecular genetic improvement allows the detection is carried out at the level of DNA, so the results more accurate, although processed. Research on several meat have been conducted by several researchers using DNA mitochondrial DNA (mtDNA). Using mtDNA because it has high copy number in a cell, compared with nuclear DNA. In this case where the amount of extracted DNA is very small or degraded. Gene used as marker specific species is cytochrome β (cyt β) because it has varied sequences. The objective of this research was to study species authentication (i.e. dog, cat, and tiger) to ensure animal origin in product using cyt β gene specific marker. Total DNA samples for each species which identified are three samples were consisting blood (cat), cooked meat (dog), feces (tiger), and as a comparison used blood sample from several animal (i.e. goat, chicken, cattle, sheep, horse, rat) and pork. Reverse primer specific for dog, cat, and tiger was designed using MEGA 5 software. DNA extraction and fragment amplification was conducted using phenol-chloroform and multiplex PCR method, respectively. Multiplex PCR is a variant of PCR which many primers were used together for amplification of multiple region. This research showed that different length of amplification for the species tested (dog, cat, and tiger) were 523, 331, 319 bp, respectively; and for the comparison species (goat, chicken, cattle, sheep, pig, horse, and rat) were 157, 227, 274, 331, 398, 439, and 603 bp, respectively. Species specificity also indicated by high reverse primer homology percentage. Specific fragment amplification on genome pool with closed fragment length should be separate to avoid band overlapped between species. Taxonomy hierarchy (i.e. dog, cat, and tiger) could be distinguished to the species level.
Keyword: cat, cytochrome β gene, dog, multiplex PCR, tiger

Judul: Sensitivitas gen sitokrom B (Cyt b) sebagai marka spesifik pada genus Rattus dan Mus untuk menjamin keamanan pangan produk asal daging
Abstrak: Appropriate techniques for detection and identification of origin of species for meat source are very important in the meat product processing to ensure the safety and halal foods. Multiplex PCR, in which many primers were used together for amplification of multiple target regions, is hopefully a suitable technique for meat sources identification. The aim of this study was to determine the specificity and sensitivity of the cytochrome b (cyt b) gene in rats as well as to design primers that can be used for detection of rat meat contamination in some processed meat products. The amplification of cyt b genes was done in seven species with different lengths of fragments which indicated specificity for cyt b genes sequences between species. The products showed specific DNA fragments of 157, 227, 274, 331, 398, 439 and 603 bp from goat, chicken, cattle, sheep, pig, horse and rat meats, respectively. Cyt b gene is sensitive as a specific marker with rats DNA that could be detected and be amplified with 100% success from as low as 1% level of contamination. Results showed that rat DNA in meatballs could be detected from 1% level contamination, otherwise rat DNA in meat floss could be detected from above 2.5% level contamination.
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Judul: Karakteristik lompatan hidrolik terhadap pelimpah terjunan pada saluran segi empat
Abstrak: Aliran dengan kecepatan tinggi dalam kondisi superkritis yang melintasi bangunan pelimpah, sebelum bergerak lebih jauh ke arah hilir saluran dimana bilangan Froude (F) lebih besar dari satu harus diubah menjadi aliran subkritis dimana bilangan Froudenya lebih kecil dari satu. Aliran yang meninggalkan kaki pelimpah merupakan aliran yang memiliki kandungan energi yang amat berarti sehingga dapat menimbulkan pengikisan yang besar. Pengubahan bilangan Froude dari kondisi superkritis ke subkritis dimaksudkan agar terjadi perubahan kandungan energi, sehingga aliran menjadi non erosive. Agar daya erosive aliran dapat berkurang, maka biasanya pada bagian hilir dari bangunan pelimpah dibuatkan suatu bangunan peredam energi. Erosi yang terjadi di hilir bangunan pelimpah dapat disebabkan dari keberadaan bangunan pelimpah itu sendiri, maupun bangunan peredam energi yang kurang tepat. Hal ini terutama menyangkut beberapa kejadian lompatan hidrolik yang timbul pada bangunan peredam energi tersebut.. Suatu metode yang efektif untuk menjamin bahwa lompatan hidrolik selalu terjadi pada daerah yang dilindungi, adalah dengan menggunakan bangunan peredam energi yang terdiri dari beberapa tipe dengan..dst
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