Judul: Dinamika Kandungan Bahan Organik (BOD) di Kalimalang Bekasi
Abstrak: Kalimalang is downstream such as from the West Tarum Canal, crosses Karawang, Bekasi, and DKI Jakarta (East Jakarta) regional. Kalimalang has suffered from pollution, especially the organic pollution upstream. This study aims to determine the dynamic of organic matter (BOD) each season, and its related the supporting parameters such as temperature, DO, TSS, pH, coliform, and debit water. In addition, STORET index is also used to determine the status of water quality. Organic matter highest in the dry season with a BOD value of 15.6 mg / l and lowest in rainy season with a BOD value of 4.89 mg / l. The high BOD value indicates that the waters are polluted It is also supported by STORET index value that is worth more than -21, which means that these waters are heavily polluted so it is not suitable for class status B.
Keyword: 

Judul: Dinamika Kandungan Bahan Organik dan Status Mutu Air Danau Garden House, Pantai Indak Kapuk, Jakarta Utara
Abstrak: Danau Garden House merupakan salah satu danau hias yang terdapat di kawasan Pantai Indah Kapuk, Jakarta Utara. Letak Danau Garden House yang dikelilingi perumahan berpotensi menerima limpasan limbah domestik yang cukup tinggi yang akan mempengaruhi kualitas air perairan tersebut. Penelitian ini bertujuan untuk menganalisis dinamika kandungan bahan organik dan status mutu air pada musim yang berbeda. Penelitian ini dilaksanakan pada bulan Juli 2017 hingga bulan Juni 2018. Analisis data terdiri atas dinamika kandungan bahan organik dan status mutu air. Status mutu air dianalisis dengan indeks Canadian Council of Minister of the Environment (CCME). Hasil penelitian menunjukkan adanya perbedaan dinamika kandungan bahan organik pada musim yang berbeda. Terdapat lima parameter kualitas air yang tidak memenuhi baku mutu kelas II menurut PPRI No. 82 Tahun 2001. Parameter tersebut ialah BOD, COD, P Total, dan hidrogen sulfida. Amonia dan ortofosfat termasuk parameter yang tidak memenuhi baku mutu untuk biota laut menurut Kepmen LH No. 51 Tahun 2004. Status mutu air berdasarkan indeks CCME menunjukkan kondisi perairan sangat buruk (poor) baik pada musim kemarau maupun musim hujan.
Keyword: bahan organik, indeks CCME, limbah, status mutu air

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
Keyword: