Judul: Amplifikasi Gen Bar dan Gen Hpt Terkait Stabilitas Padi Mutan Serta Evaluasi Karakter Agronominya
Abstrak: Transposon Ac / Ds system containing an activation tagging constructs serves to develop a mutant plant that will produce changes in phenotype. This study aims to amplify the bar and hpt genes related to the stability of the mutant rice transposon Ac/Ds and observe the changes in phenotype based on the agronomy characters. Rice plant materials used for the study were six mutant T2 rice lines IR.64 (total 214 plants) and IR64 wild type rice as a control. The DNA of mutant plants was isolated and then amplified by PCR technique using primers specific for the bar and hpt genes. The results showed that the bar and hpt genes in the rice plant IR.64 mutant T2 with size were ± 700 bp and ± 600 bp. Evaluation of agronomic characters showed there were changes in these characters of mutant rice lines IR64 generation T2 that probably caused by the transposon insertion element containing 4x enhancer.
Keyword: Bogor Agricultural University (IPB), IR64., agronomic traits, Ac / Ds transposon

Judul: Deteksi Gen hpt dan bar untuk Uji Kestabilan Transposon Ac/Ds pada Padi Mutan IR64
Abstrak: Ac/Ds transposon system can be used to gain variety of mutant rice genotype and for identifying the function of mutant gen especially IR64 rice. However to obtain mutant plant that we want, stable transposon is required in order to avoid transposon movement to other chromosoms. This research has analyzed 26 samples of genom DNA of first generation (T0) plants with polymerase chain reaction (PCR) using bar and hpt primer also Southern hybridization analyzing. There is 26 genom DNA samples of second generation (T1) plants analyzed with PCR. PCR analyzation showed that transposon Ac/Ds is occur in all samples. Southern hybridization showed that 14 of 26 T0 plants contain single copy of T-DNA. Fourteen T1 generation plants contain stable Ds transposon based on the results of PCR analysis. This research proved that Ds transposon is stable because it was inherited on the next generation
Keyword: stability analysis, Ac/Ds transposon, IR64

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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