Judul: Perencanaan Jalur Interpretasi Anggrek di Hutan Wisata Taman Eden, Toba Samosir
Abstrak: This study aimed to develop a general plan of orchids interpretive trail at Eden Park Tourism Forest (EPTF). Data was collected through literature review, interviews, distribution of questionnaires, and field observations. Inventory of the region showed that orchid plants were easily found in all four observation trails, with a total of 51 species from 29 genera. Visitors of EPTF was dominated by male visitors (63%) with majority of age group of 20-30 years (48%). Most visitors (64%) preferred trail 1 as interpretive trail for orchids. Trail 1 was then chosen as the trail to be developed into interpretive trail based on its characteristics suitability for such trails, its orcgids potentials, and visitors preference toward the trail. Planning of interpretive trail included program planning, and supporting facilities planning. The programs were developed under the following themes: Paphiopedilum tonsum orchid is very rare species, so it must be protected for its existence and cultivation is one of the activity for orchid plant preservation.
Keyword: visitor, orchids, interpretive trail, forest, Eden Park Tourism Forest

Judul: Perencanaan Jalur Interpretasi Kawasan Hutan Pendidikan Gunung Walat Sukabumi, Jawa Barat
Abstrak: Gunung Walat Education Forest (GWEF) is a forest area which have potential objects and trails can be developed as interpretation trail. The aims were to identify potential interpretation objects and condition at eight trails in GWEF, to identify visitor’s preferences, and planned the landscape interpretation trail of GWEF. Data analyzed using the criteria tool analysis developed by Veverka (1998), scoring, and descriptive. The result is that on eight trails consist of 24 plant objects, 19 animal objects, five physical objects, a historical and ancient site object. Plant object become main visitor’s preference amount 40%. There ware eight interpretation trail which consist of three classification are as follow high potential (two trails), medium potential (one trail), low potential (five trails).
Keyword: Interpretation trail., Gunung Walat Educational Forest (GWEF)

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
Keyword: