Judul: Pengaruh waktu dan penggunaan briket kompos terhadap sifat fisika tanah dan pertumbuhan jagung manis pada pengolahan minimum latosol sindang barang
Abstrak: Limbah organik seperti campuran kotoran hewan dan limbah kayu pulai (Alstonia scholaris) dapat dimanfaatkan menjadi media tanam briket kompos melalui proses pengomposan dan pemadatan. Penggunaan briket kompos sebagai media tanam untuk pembibitan langsung di lahan pertanian, memungkinkan benih dapat hidup dalam kondisi yang optimum dengan meminimumkan pengolahan tanah, pemberian air dan pupuk. Penelitian ini bertujuan mempelajari pengaruh waktu penggunaan media tanam briket kompos terhadap sifat fisika tanah dan pertumbuhan tanaman jagung manis (Zea mays saccharata) pada pengolahan minimum Latosol Sindang Barang. ...
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Judul: Pengaruh pengolahan tanah dan pemberian bahan organik terhadap beberapa sifat fisik tanah dan produksi jagung manis (Zea mays saccharata) pada latosol Jampang Tengah Sukabumi
Abstrak: Penelitian ini dilakukan di lahan milik Kelompok Tani Sadagori di Kampung Nangela, Kecamatan Jampang Tengah, Kabupaten Sukabumi. Penelitian ini bertujuan untuk mengetahui pengaruh pengolahan tanah dan pemberian bahan organik terhadap beberapa sifat fisik tanah dan produksi jagung manis. Penelitian ini dilakukan dengan memberikan perlakuan yang terdiri dari 4 jenis pengolahan tanah dan 3 taraf pemberian bahan organik. Keempat jenis pengolahan tanah tersebut yaitu 1) tanpa pengolahan tanah (PO); 2) pengolahan minimum (P1) yaitu pengolahan tanah selebar 20 cm di kanan-kiri baris tanaman sedalam 20 cm dengan pencangkulan; 3) pengolahan tanah 20 cm yaitu pengolahan tanah dengan pencangkulan sampai kedalaman 20 cm pada seluruh permukaan tanah; dan 4) pengolahan tanah intensif (P3) yaitu pengolahan tanah sedalam 20 cm lalu digemburkan dengan mencangkul beberapa kali. Ketiga taraf pemberian bahan organik adalah: 1) 0 ton/ha (K1), 2) 15 ton/ha (K2), dan 3) 30 ton/ha (K3). Pemberian bahan organik berupa kotoran domba yang telah matang (kering) dilakukan dengan ..dst
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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