Judul: Peranan Kepemimpinan Desa dalam Perubahan Struktural Masyarakat Pedesaan
Abstrak: Aspek aspek pokok perrnasalahan penelitian ,meliputi:(1) Pengaruh peranan kepeniimpinan Kepaia Oesa terhadap peranan kepemimpinan pemimpin masyarakat dan pengaruh peranan lcepemimpinan masyarakat terhadap perubahan peranan anggota masyaraltat dalam berbagai aspek kelembagaan serta perubahan status sosial ekonomi anggota masyarakat , (2)Peranan managerial Kepala Desa dan aktivitas peranan pemimpin masyarakat, jarak sosial dan illt egrasisosial Kepa1,a Desa dengan p~amimpin masyaraltat dan pemimpin masyaraltat dengan ailggota masyarakat , (3)Pengaruh faktor kondisi masyarakat; faktor ekstern desa dan kemampuan individual (peribadi) masing masing terhadap peranan kepemimpinan Kepala Desa, terhadap peranan ltepemimpinan pemirnpin masyarakat dan terhada perubahan peranan dan perubahan status sosial ekonomi anggota masyarakat .
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Judul: Peranan Kepala Desa sebagai Penggerak Peran Serta Anggota Masyarakat dalam Pembangunan Fisik Desa
Abstrak: Penelitian ini bertujuan untuk mengetahui: (1)peranna kepala desa, cara peranan itu dilakukan dalam pembangunan fisik dan pengruh faktor-faktor yang ada pada dan diluar dirinya terhadap peranan tersebut ; (2) peranan pemimpin informal, mengulas peranna dalam pembangunan fisik; (3) kaitan antara kepala desa, pamong desa dan pemimpin informal dalam pembangunan fisik, khususnya dalam menggerakan peran serta anggota masyarakat; (4) pengaruh tingkat sosial ekonomi anggota masyarakat, persepsinya dan tradisi masyarakat terhadap peran sertanya dalam pembangunan fisik desa.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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