Judul: Efektivitas spawnprim sebagai pemercepat Ovulasi pada ikan komet Carassius auratus auratus
Abstrak: Penggunaan ovaprim dalam pemijahan buatan telah mampu merangsang ikan agar berovulasi lebih cepat dibandingkan pemijahan secara alami. Namun demikian, harga ovaprim yang relatif tinggi berdampak pada biaya produksi benih yang tinggi pula. Pada penelitian pemijahan buatan menggunakan ikan komet, digunakan campuran bahan (spawnprim) aromatase inhibitor (AI), anti dopamin (AD) dan LHRH analog (LHRHa) sebagai pemicu ovulasi sekaligus menggantikan peran ovaprim. Pengaruh spawnprim ternyata dapat memicu ikan berovulasi pada jam ke-6 setelah penyuntikan. Hasil yang sama didapat pada perlakuan menggunakan ovaprim. Perbedaan signifikan hanya terlihat pada parameter tingkat ovulasi. Tingkat ovulasi yang dicapai spawnprim berkisar antara 5,98 ± 2,83% hingga 13,88 ± 2,75% dan belum mampu menyamai pencapaian ovaprim yaitu sebesar 29,44 ± 6,80%. Hal ini diduga karena pengaruh kadar AI yang terlalu tinggi (150 mg/l) dalam spawnprim. Dugaan lain adalah ikan mencapai ovulasi lebih cepat, namun keterlambatan striping menimbulkan atresia pada telur. Hasil penelitian ini menunjukkan bahwa spawnprim dapat memicu percepatan ovulasi pada ikan komet. Penggunaan spawnprim dalam kegiatan pemijahan buatan merupakan alternatif untuk menekan biaya produksi benih sehingga dapat lebih efisien.
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Judul: Efektivitas Spawnprim pada proses ovulasi dan pemijahan ikan komet Carassius auratus auratus
Abstrak: Artificial propagation is required in particular for fish that difficult to spawn naturally in the captivity. Spawning by hormonal manipulation is one of the techniques that is often done in the hatchery fish. The most common hormone used this technique is Ovaprim (20 μg/mℓ sGnRH-a + 10 mg/mℓ anti-dopamine, AD). Recently, it was also found other materials that can stimulate spawning namely aromatase inhibitor (AI). The addition of AI in hormonal compound is expected to stimulate spawning fish more effectively and efficiently. This study aimed to test the effectiveness of Spawnprim (LHRH-a + anti-dopamine + AI) in stimulating spawning in female comet-fish as a model fish. Spawnprim was designed into four different treatment combinations (A, B, C, and D) using 0- 15μg/mℓ LHRH-a. Ovaprim was used as a positive control whereas physiological solution without any hormone was used as a negative control. Six female of comet fish were injected with each hormonal treatment with a dose of 0.5mℓ/kg body weigth. The results showed that Spawnprim A, B, C and D could stimulate ovulation with the same success rate as the Ovaprim which was 100% in the latent period of 6 hours post-injection, whereas control negative showed no ovulation. Spawnprim C and D did not show a significant difference in ovulation rate with Ovaprim (p>0,05). Spawnprim A, B, C and D give the same results with Ovaprim for egg diameter, fertilization level, hatching level, and survival rate of larvae at the age of 4 days (p>0,05). AI may replace the role of LHRH in stimulating final maturation process of gonads. Spawnprim C and D can be as effective as Ovaprim in the final maturation of the gonads, and yet more economical.
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Judul: Floristic and phytosociology of bryophytes of the Lore Lindu National Park, Sulawesi
Abstrak: Sulawesi has a high biodiversity of phanerogams and high degree of endemism. Phytogeographically Sulawesi is part of East Malesia; it is separated from the West Malesia by the Wallace's line. As compared to adjacent major island in Malesia, the number of bryophytes species which were found in Sulawesi is low.
Keyword: Bryophytes, phytogeography, Wallace's line, forest disturbance, Lore Lindu National Park, Wallace's line, Forest disturbance, Lumut, Fitogeografi, Keerusakan hutan, Taman Naasional Lore Lindu