Judul: The Effects of Thiobacillus sp. Probiotics Application to  Water Quality of Shrimp Pond Wastewater
Abstrak: Senyawa beracun seperti H2S, amonia, nitrit, dan nitrat di tambak dapat berasal dari perombakan bahan organik yang tertimbun di sedimen. Usaha yang dilakukan untuk menciptakan kualitas air yang baik, salah satunya adalah dengan aplikasi bakteri probiotik. Penelitian ini bertujuan untuk menganalisis pengaruh penerapan bakteri probiotik Thiobacillus sp. terhadap kualitas air pada limbah tambak. Penelitian ini menggunakan rancangan RAL in time dengan empat perlakuan. Perlakuan yang diberikan yaitu penambahan konsorsium Thiobacillus sp. dengan dosis 0 ppm (kontrol), 0,1 ppm, 0,2 ppm, dan 0,4 ppm. Parameter yang diamati yaitu suhu, pH, DO, amonia, nitrit, nitrat, H2S, serta penghitungan kelimpahan total bakteri. Hasil menunjukkan bahwa penambahan probiotik yang mengandung Thiobacillus dapat meningkatkan kelimpahan total bakteri pada air limbah. Penambahan probiotik memberi pengaruh terhadap penurunan konsentrasi nitrit dan nitrat pada beberapa perlakuan. Namun, penambahan probiotik tidak berpengaruh pada penurunan konsentrasi H2S dan TAN., Toxic compounds such as H2S, ammonia, nitrite, and nitrate in ponds can come from the breakdown of organic matter in ponds sediments. Good ponds water quality could be created by the application of probiotic bacteria. This study aims to analyze the effect of Thiobacillus sp. probiotic application on water quality of pond wastewater. This study used the RAL design in time with four treatments. The treatment given was the addition of a consortium of Thiobacillus sp. with doses of 0 ppm (control), 0.1 ppm, 0.2 ppm, and 0.4 ppm. The parameters observed were temperature, pH, DO, ammonia, nitrite, nitrate, H2S, and the abundance of total bacteria. The results showed that the addition of probiotics containing Thiobacillus could increase the total bacteria in wastewater. The addition of probiotics had an effect on decreasing of nitrite and nitrate concentration in several treatments. However, the addition of probiotics had no effect on decreasing of H2S and TAN concentration.
Keyword: probiotic, Thiobacillus, wastewater, water quality

Judul: Analysis of the Effect of Bacillus sp. Probiotic Application for Controlling Vibrio spp. Bacterial Population in Vannamei Shrimp (Litopenaeus vannamei) Cultivation.
Abstrak: Bakteri Vibrio spp. merupakan salah satu jenis bakteri patogen yang sering ditemukan di lingkungan perairan, seperti pada budidaya udang. Infeksi Vibrio spp. mampu menyebabkan beberapa penyakit pada udang seperti vibriosis, Acute Hepatopancreatic Necrosis Disease (AHPND), dan feses putih. Pengendalian jumlah populasi dan infeksi oleh Vibrio spp. dalam budidaya udang dengan penggunaan probiotik Bacillus sp. pada pakan udang. Bakteri Bacillus sp. memiliki kemampuan anti quorum sensing dengan mengganggu acyl homoserine lactones (AHL), sehingga AHL Vibrio spp. tidak mencapai kondisi kuorum. Penelitian ini bertujuan menganalisis pengaruh aplikasi probiotik Bacillus sp. untuk pengendalian populasi bakteri Vibrio spp. pada budidaya udang vaname. Penelitian dilakukan hingga Day of Culture (DOC) 120 pada 6 kolam tambak terletak di PT. Sentra Budidaya Biotek, Kabupaten Sumbawa Barat, Nusa Tenggara Barat. Metode yang digunakan terdiri dari persiapan tambak, aklimatisasi benur, perbanyakan dan aplikasi probiotik Bacillus sp., Pemeriksaan kualitas air, isolasi dan perhitungan bakteri Vibrio spp., uji histopatologi hepatopankreas udang, sampling udang, dan panen udang. Aplikasi probiotik Bacillus sp. memberikan dampak positif terhadap pengendalian populasi bakteri Vibrio spp., kualitas air tambak, uji histopatologi hepatopankreas udang, dan hasil panen udang. Hal ini ditunjukkan dengan populasi Vibrio spp. dibawah ambang batas, terkendalinya kualitas air, terkendalinya infeksi dari Vibrio spp. pada hepatopankreas udang dan jumlah produksi udang mencapai target., Vibrio spp. are a type of pathogenic bacteria commonly found in aquatic environments, such as in shrimp pond. Infections by Vibrio spp. can lead to several shrimp diseases such as vibriosis, Acute Hepatopancreatic Necrosis Disease (AHPND), and white feces syndrome. Controlling the population and infections of Vibrio spp. in shrimp pond can be achieved through the use of Bacillus sp. in shrimp feed. Bacillus sp. have anti-quorum sensing abilities by disrupting acyl homoserine lactones (AHL), preventing Vibrio spp. from reaching quorum conditions. This study aims to analyze the effects of Bacillus sp. controlling the population of Vibrio spp. in vannamei shrimp pond. The research was conducted until Day of Culture (DOC) 120 in 6 ponds located at PT. Sentra Budidaya Biotek, West Sumbawa, West Nusa Tenggara. The methods included pond preparation, shrimp acclimatization, Bacillus sp. culture and application, water quality assessment, isolation and quantification of Vibrio spp., histopathological examination of hepatopancreas, shrimp sampling, and harvest. The application of Bacillus sp. showed positive impacts in suppressing Vibrio spp. populations, maintaining pond water quality, reducing Vibrio spp. infections in shrimp hepatopancreas, and shrimp production. This was evidenced by Vibrio spp. populations staying below threshold limits, controlled water quality, controlled infections from Vibrio spp. in shrimp hepatopancreas, and achieving the targeted shrimp production.
Keyword: Aplikasi, Litopenaeus vannamei, anti-quorum sensing, Bacillus  sp., Vibrio spp.

Judul: Klasifikasi Sidik Jari Menggunakan Support Vector Machine Dengan Feature Berbasis Minutiae Menggunakan Metode Region
Abstrak: Manual fingerprint classification proceeds by carefully inspecting the geometric characteristics of major ridge curves in a fingerprint image. This research proposed an automatic approach of identifying the geometric characteristics of ridges based on minutiae position and angle. Position and angle of minutiae are analyzed using region method. Region was used to discretize the number of minutiae. Positions of minutiae in a region give information about its relation to another region. Angles of minutiae indicate the ridge flow direction. Support vector machine, a binary classifier, is used to classify the fingerprint based on those characteristics. In this research, the classes are left loop, right loop, whorl, arch and tented arch. This research used 2695 fingerprint images from NIST. Classification performance is measured using 3-fold cross validation method. This research achieved 79.7 % accuracy. As we only used local features in this research, further research needs to be conducted especially to investigate global features.
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