Judul: Kajian Metode Penggerombolan Dua Tahap untuk Data yang Mengandung Pencilan
Abstrak: Cluster analysis is often encountered in various studies. Analysis of classical clusters, such as hierarchical clustering method and k-means clustering cannot handle categorical variables or a mixture of numerical and categorical. In addition, the determination of the optimal number of clusters are still dependent on the subjectivity of the researcher and cannot handle very large datasets, which is larger than 500. One approach to addressing this problem is to use a two-step clustering method. The accuracy of the two-step clustering method of predicting the number of clusters generated as well as the classification of cluster membership, especially in the data containing outliers is important to be studied. Outliers in the data containing a small (1%), this method provides more accurate compared with the results of data containing a large outliers (5% or 15%). Scale use of outliers handling in the data containing outliers must be greater than the amount of outliers itself. Two-step clustering method is very accurate in producing a number of clusters associated with the actual number of population clusters that do not contain data outliers, especially in the most variable of type numeric and categorical rest. Clustering villages in Indonesia by a factor of progress and backwardness villages using a two-step clustering method generates optimal cluster 7.
Keyword: two-step clustering method, data outliers, cluster analysis

Judul: Perbandingan Hasil Penggerombolan Metode K-Means, Fuzzy K-Means, dan Two Step Cluster
Abstrak: The main principle of cluster analysis is to classify objects into clusters based on similarity measures. K-means and fuzzy k-means can be classified as popular clustering methods, which are suitable for large data with continuous variables. However, a new method has been developed to be used for large data, that is the two step cluster method. This method allows processing data with different types of variables, which in this case are continuous and categorical. The aim of this research is to compare the clustering results of k-means, fuzzy kmeans, and two step cluster method, in order to determine the ideal number of clusters for each method. This research uses hypotetical data taken from SPSS software, which fit the purpose to compare several methods. The results of this study show that in the case of two clusters, k-means and fuzzy k-means methods have more similarities with respect to the number objects in clusters, whereas the two step method gives unequal number of objects in clusters. All methods show that 2 clusters is an ideal number. It is influenced by the ratio between mean squares within clusters, which is smaller than the ratio in the case of 3 and 4 clusters.
Keyword: 

Judul: Co-infection of Infectious Myonecrosis Virus (IMNV) and Vibrio harveyi in Pacific White Shrimp (Litopenaeus vannamei)
Abstrak: L. vannamei production in Indonesia was growing intensively. In 2006, IMN (infectious myonecrosis) disease was confirmed spread to Indonesia shrimp farm and declined shrimp production. The study was conducted to investigate effect of dose of V. harveyi on co-infection with IMNV in L. vannamei as well as development of viral infection. Shrimps juvenile were oral infected with IMNV infected shrimps 10 % feeding rate during 3 days and co-infected with 106, 107 and 108 cfu/ml V. harveyi. Mortality rate was 0 % in control and single infection of V. harveyi except 108 cfu/ml treatment. Mortality pattern demonstrated on co-infection was faster and higher than single IMNV infection in 14 days observation. The density of green colony Vibrio in hepatopancreas of co-infected shrimps collected in 2, 4, 6, 8 and 10 days post infection were higher than V. harveyi single infected (significantly in 10 days post infection). There were no difference of IMN disease development between co-infection and IMNV single infection. It was confirmed by visual gross sign appeared, tissue and lymphoid organ histophatology, organ abnormality, and PCR test. In conclusion, IMN disease caused higher and faster mortality on co-infection with V. harveyi, but not affect to IMN disease development.
Keyword: