Judul: Analisis Segregasi Gen hpt (Hygromisin Phosphotransferase) pada Tanaman Tembakau Transgenik Generasi T0
Abstrak: Hygromycine phosphotransferase encoded by hpt gene is one of commonly used as a selectable marker for the production of transgenic plants. The plants harbored the hpt gene were able to synthesize hygromycine phosphotransferase enzymes, therefore they are tolerant to higromicine.The objective of this research is to analyze the segregation of hpt gene in T0 generation transgenic tobacco plants. T0 transgenic plants were cultivated in the pot and placed in the green house. The seeds produced by selfing of T0 plants were cultivated on the MS0 media containing 50mg/l higromycine for one month. The rasio between the number of tolerant and sensitive plants to higromycine is 3:1 based on Khi-square. This result indicated that hpt gene is integrated in genom of T0 plants, inherited as Mendelian pattern for one gene, and the genotype of T0 plants is heterozigous for hpt gene. As hpt gene linked to MmCuZn-SOD gene, so T0 plants contain also MmCuZn-SOD gene.
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Judul: Analisis integrasi gen hpt (hygromycin phosphotransferase) di tanaman kentang (Solanum tuberosum L.) kultivar diamant transgenik
Abstrak: Perbaikan genetik tanaman dapat dilakukan dengan rekayasa genetika. Hd3a dapat menginduksi pembungaan dan pembentukan umbi pada kentang. Tanaman kentang (Solanum tuberosum L.) kultivar Diamant transgenik yang mengandung gen Hd3a di bawah kendali promoter kuat rolC yang dipautkan dengan gen hpt (hygromycin phosphotransferase) dapat dianalisis dengan menggunakan PCR (Polymerase Chain Reaction). Analisis PCR dengan menggunakan primer Hp-F dan Hp-R digunakan untuk mengetahui integrasi gen hpt di dalam tanaman kentang transgenik. Analisis PCR menunjukkan bahwa 12 klon transgenik putatif yang resisten terhadap higromisin, mengandung gen hpt yang ditandai oleh pita DNA berukuran 570 pb. Gen hpt terpaut dengan gen Hd3a, sehingga klon transgenik tersebut juga mengandung gen Hd3a. Dengan menggunakan bibit Go, dua klon transgenik, DIT1 dan DIT2, telah ditanam di polybag. Tanaman DIT1 mengalami pembungaan dini pada 45 Hari Setelah Tanam (HST) sedangkan tanaman kentang lainnya tidak berbunga. Hal ini menunjukkan bahwa Hd3a mampu menginduksi pembungaan pada kentang kultivar Diamant transgenik. Bobot umbi DIT1 lebih tinggi dibandingkan dengan tanaman DI (non-transgenik) yang menunjukkan bahwa Hd3a juga berpotensi meningkatkan produktivitas tanaman kentang kultivar Diamant.
Keyword: Gen Hd3a, Gen hpt, Polymerase Chain Reaction, Promoter rolC, Kentang kultivar diamant transgenik, Biological science, Kentang kultivar, Isolasi DNA genom

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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