Judul: Analisis Potensi Daun Torbangun (Plectranthus amboinicus) sebagai Antibakteri terhadap Klebsiella pneumoniae secara in silico
Abstrak: Resistensi antibiotik adalah masalah global. Bakteri Klebsiella pneumoniae merupakan bakteri prioritas untuk ditemukan antibiotiknya karena adanya enzim extended spectrum beta-lactamase (ESBL) yang menyebabkan bakteri ini mudah mengalami resistensi. Daun torbangun diketahui memiliki banyak sekali kandungan flavonoid yang sering diteliti sebagai antibakteri. Penelitian ini bertujuan menganalisis potensi daun torbangun (Plectranthus amboinicus) sebagai antibakteri terhadap Klebsiella pneumoniae dengan metode in silico. Penelitian dilakukan dengan melakukan penambatan molekuler menggunakan aplikasi AutoDock Vina terhadap enzim dari bakteri Klebsiella pneumonia yaitu SHV (Sulfhydryl variable) dengan senyawa kimia yang diambil dari tanaman daun torbangun. Parameter yang digunakan adalah energi bebas ikatan, konstanta inhibisi, serta visualisasi ligan secara 3D dan 2D. Hasil yang diperoleh menunjukkan ligan alami memiliki energi bebas ikatan sebesar -6,7 kcal/mol dan konstanta inhibisi 12,11 µM. Nilai energi bebas ikatan dan konstanta inhibisi dari luteolin 3’Oglucuronide, apigenin 7-glucuronide, dan asam rosmarinat menunjukkan hasil yang lebih baik dibandingkan dengan ligan alami dari SHV-1 (1,4-thiazepine)., Antibiotic-resistant is a global issue. Klebsiella pneumoniae has high priority to find its antibiotic because it has extended-spectrum beta-lactamase (ESBL) enzyme that makes the bacteria easy to resist the antibiotic. Indian borage is one of the empirical medicinal plants that contain flavonoids being used for treating the disease. This research had the purpose of analyzing Indian borage potential as an antibacterial for Klebsiella pneumoniae in silico. Docking is the method for this research by using AutoDock Vina, an application to attach enzyme ESBL from Klebsiella pneumoniae which mention as SHV (Sulfhydryl variable) and metabolite seconders from the plant. The analyzed parameter was binding free energy, inhibition constant, and visualization in 3D and 2D. Binding free energy and inhibition constant of a native ligand is -6.7 kcal/mol and 12.11 µM. Binding free energy value of apigenin 7-glucuronide and rosmarinic acid perform higher potency than the native ligand of SHV-1 (1,4-thiazepine).
Keyword: Daun torbangun, Klebsiella pneumoniae, Resistensi antibiotik, Penambatan molekuler

Judul: Potensi Senyawa Aktif Sungkai (Peroema canescens) dalam Menginhibisi Enzim Beta-Laktamase Secara In Silico.
Abstrak: Kasus resistensi bakteri terhadap antibiotik menjadi ancaman serius bagi kesehatan manusia karena menurunkan efektivitas obat untuk mencegah dan menyembuhkan infeksi bakteri. Resistensi dapat disebabkan oleh pengobatan yang tidak tuntas. Salah satunya yaitu bakteri Escherichia coli yang menghasilkan enzim CTX-M yang merupakan jenis dari beta-laktamase yang menghambat kerja antibiotik golongan beta-laktam. Sungkai merupakan salah satu tanaman obat tradisional dan terbukti menghambat pertumbuhan bakteri Escherichia coli. Penelitian ini bertujuan mengidentifikasi senyawa aktif sungkai yang berpotensi menginhibisi beta-laktamase serta analisis interaksi yang terjadi secara in silico. Senyawa aktif daun sungkai ditambatkan ke reseptor beta-laktamase, kemudian dianalisis interaksinya serta diprediksi bioavailabilitas dan toksisitas. Hasil penelitian menunjukan sebanyak 6 dari 19 ligan uji lolos penapisan hingga akhir dan berpotensi menjadi inhibitor beta-laktamase. Senyawa tersebut antara lain, beta-sitosterol, 3,4’,5-thrihydroxy-3,’,7-dimenthoxyflavanone, physcion, isorhamnetin, chrysoeriol, quercetin 3,3 dimetly ether, dan vintexin-2-O rhamnoside., Cases of bacterial resistance to antibiotic has become a serious threat for human health because they reduce the effectiveness of drugs in preventing and treating bacterial infections. Resistance can be caused by uncomplete treatment. One of example is Escherichia coli bacteria which produces the CTX-M enzyme, that is a type of of beta-lactamase that inhibits the action of beta-lactam antibiotics. Sungkai is one of the traditional medicinal PLANTS and it is proved inhibiting the growth of Escherichia coli. This research aims to identify the active compounds of sungkai that have the potential to inhibits beta-lactamase and analyze interactions of molecular docking. The active compounds of sungkai leaves are docked to the beta-lactamase receptor, then their interactions are analyzed, predicting bioavailability, and toxicity. The results showed that 6 of the 19 tested ligands pass the screening and have the potential to become beta-lactamase inhibitors. The compounds are beta-sitosterol, 3,4’,5-thrihydroxy-3’7-dimenthoxyflavanone, physcion, isorhmaetin, chrysoeriol, quercetin 3,3 dimetly ether, and vintexin-2-O-rhamnoside.
Keyword: resistent, beta-lactamase inhibitors, sungkai

Judul: Dampak Perceraian dan Hubungan Sosial terhadap Kesejahteraan Psikologis Ibu sebagai Orang Tua Tunggal
Abstrak: Family with a single mothers who came from divorced, often bring the impact such as problem or change on a single mothers well-being. The aim of this research to indentify characteristics of single mother and relations with psychological well-being and social relations, and also analyze impact divorce on psychological well-being and social relations. Descriptive analysis, regression, and Pearson correlation was used in this study. The respondents were sixty single mother who come from the length divorced more than six month. The result showed that compare with present condition, psychological well-being, impact of divorce and social relation respondent are more significant lower than the first six month after divorce. There was relationship between age of present and divorce, family’s income, and size of family with level of depression, social support, and economic impact. Based on regression analysis we found there was negative effect of impact of divorce to psychological well-being at the first six month after divorce and the present. Other wise, we found there was not significant effect of divorce to social relationship.
Keyword: economic impact, level of depression, social support