Judul: Gender Roles in Childcare and Check-ups at Posyandu also the Quality of Care for Stunted Children
Abstrak: Kurangnya keterlibatan ayah dalam perawatan dan pemeriksaan anak ke posyandu menjadi faktor yang menentukan kualitas perawatan anak stunting. Tujuan disusunnya penelitian ini untuk menganalisis peran gender dalam perawatan, peran gender dalam pemeriksaan anak ke posyandu, dan kualitas perawatan anak stunting. Pengambilan contoh menggunakan non-probability sampling dengan menggunakan teknik purposive sampling. Subjek penelitian ini adalah orang tua dari anak stunting di Desa Wanamekar, Kecamatan Wanaraja, Kabupaten Garut. Terdapat hubungan antara asuransi anak dengan peran gender pemeriksaan anak ke posyandu, peran gender dalam perawatan dengan pemeriksaan anak ke posyandu serta kualitas perawatan anak stunting. Terdapat pengaruh signifikan pada nomor urut anak kandung, usia ayah, usia ibu, pendidikan terakhir ayah, pekerjaan ibu, pendapatan ayah, banyak anggota keluarga, asuransi anak, dan peran gender perawatan terhadap kualitas perawatan anak stunting. Diperlukan kesadaran dan kerja sama sebagai orang tua dalam berbagi peran gender perawatan dan peran gender pemeriksaan anak ke posyandu untuk memutus rantai stunting dan menyiapkan sumber daya manusia berkualitas.
Keyword: gender roles, posyandu check-up, stunting, quality of care

Judul: Factors Related to the Performance of Cadres in Handling Stunting  in Pabuaran Village Bogor Regency
Abstrak: Kader posyandu merupakan ujung tombak utama dalam memasyarakatkan pengetahuan mengenai kesehatan, salah satunya terkait isu stunting. Seorang kader yang baik dapat tercermin dari kinerjanya. Akan tetapi, beberapa penelitian menunjukkan kinerja kader masih rendah. Tujuan penelitian ini adalah (1) mendeskripsikan tingkat kinerja kader posyandu dalam penanganan stunting, dan (2) menganalisis hubungan karakteristik kader, kualitas pelatihan, kompetensi komunikasi interpersonal, dan motivasi dengan kinerja kader dalam penanganan stunting. Penelitian ini menggunakan metode sensus dengan pendekatan kuantitatif dan didukung data kualitatif dengan responden sebanyak 36 orang kader posyandu di Desa Pabuaran, Kabupaten Bogor yang diambil secara sensus. Hasil penelitian menunjukkan tingkat kinerja kader posyandu dalam penanganan stunting berada pada kategori sedang. Penelitian ini juga menunjukkan bahwa pekerjaan, lama menjadi kader, tingkat pengetahuan, intensitas pelatihan, kompetensi komunikasi interpersonal, dan motivasi ekstrinsik berhubungan nyata dengan kinerja kader posyandu dalam penanganan stunting., Posyandu cadres are the main spearhead in disseminating knowledge about health, one of them is the issue of stunting. A good cadre can be reflected from their performance. However, several studies have shown that the performance of cadres is still low. The purpose of this study are to (1) describe the performance level of posyandu cadres in handling stunting, and (2) analyze the relationship between cadre characteristics, training, interpersonal communication competence, and motivation with cadres' performance in handling stunting. This study used a census method with a quantitative approach and supported by qualitative data with 36 posyandu cadres in Pabuaran Village, Bogor Regency who were taken by census. The results of the study show that the performance level of posyandu cadres in handling stunting is in the moderate category. This study also shows that job, years of service, level of knowledge, training intensity, interpersonal communication competence, and extrinsic motivation are significantly related to the performance of posyandu cadres in handling stunting.
Keyword: performance, posyandu cadres, stunting

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.