Judul: Hubungan Karakteristik Internal, Eksternal, dan Persepsi dengan Kepuasan Pendengar terhadap Program Talkshow di Radio RRI Bogor (Kasus Pelanggan PLN dan PDAM yang Mendengarkan RRI Bogor)
Abstrak: Radio Republik Indonesia (RRI) was one of many kind of tools that used in the independence period. RRI in forced in order to play the important role as a Public Broadcasting Institution, in other hand RRI also forced to gives a balance information to the society. The most important role of RRI is to extend the develompent program from the government to the society. One of the RRI’s program is Talkshow with several public institution such as PLN and PDAM. This research was conducted to answer several objectives such as: (1) To indetify the satisfaction level from the audience toward the talkshow program in RRI. (2) To analyze the connection between internal charactersitics and the satisfaction level from the audience toward RRI talkshow (3) to To analyze the connection between external charactersitics and the satisfaction level from the audience toward RRI talkshow. And the last purpose (4) to analyze the connection between the exposureness of RRI and the satisfaction level from the audience toward RRI talkshow. Data was collected using questionnaire and was taken from May – June. This research produced several findings. Finding/result from this research shows that the majority of audience aged between 39 – 52, with majority college level graduate, have been a subscriber PDAM for 8 -12 years and 2 – 11 years for PLN, listening to RRI ranged from 6 to 7 times a week with time of listening less then 130 minutes each listening activity, have a good perception toward the resource person and also the services provided by PLN and PDAM. The finding tells us, majority of audience satisfied with the talkshow program. In the internal characteristics there is a quite strong correlation between the time of subscription and the satisfaction level. Mean while in the there is also a strong correlation between the perception toward the resource person, service from PLN and PDAM with the satisfaction level.
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Judul: Analisis Kesenjangan Kepuasan (Gratification Discrepancy) : Kasus Pendengar Program Siaran “Desa Kita” Pada Radio Republik Indonesia (RRI) Bogor FM 93,75 MHz
Abstrak: Radio mempunyai peran yang strategis dalam menyampaikan pesan-pesan pembangunan. Pesan-pesan pembangunan tersebut di antaranya dalam bidang pertanian. Salah satu bentuk produk penyuluhan dan komunikasi pertanian melalui media radio adalah siaran pedesaan. Radio Republik Indonesia atau yang umum dikenal dengan sebutan RRI merupakan salah satu radio yang memiliki program siaran pedesaan yang disebut “Desa Kita”. Program siaran “Desa Kita” ini disiarkan setiap hari Selasa dan Kamis pukul 16.30 – 17.30 melalui Programa 1 RRI Bogor FM 93,75 Mhz. Untuk melihat perkembangan dan respon masyarakat, khususnya pendengar program siaran “Desa Kita” RRI Bogor ini maka perlu dilakukan pengkajian dalam hal kesenjangan kepuasan. Penelitian ini dilakukan pada Radio Republik Indonesia (RRI) Bogor yang beralamat di Jln. Pangrango No. 34, Bogor, Jawa Barat. Penelitian ini bertujuan : (1) mengkaji karakteristik, penggunaan media radio, dan kesenjangan kepuasan pendengar program siaran “Desa Kita” RRI Bogor FM 93.75 MHz; (2) menganalisis hubungan antara karakteristik pendengar program siaran “Desa Kita” RRI Bogor FM 93.75 MHz dengan penggunaan media radio; (3) menganalisis hubungan antara karakteristik pendengar program siaran “Desa Kita” RRI Bogor FM 93.75 MHz dengan kesenjangan kepuasan pendengar; (4) menganalisis hubungan antara penggunaan media radio dengan kesenjangan kepuasan pendengar program siaran “Desa Kita” RRI Bogor FM 93.75 MHz; dan (5) menganalisis kesenjangan kepuasan (gratification discrepancy) pendengar program siaran “Desa Kita” RRI Bogor FM 93.75 MHz.
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Judul: Gene Editing  of Two Cyanide Synthesis-related Genes in Cassava using CRISPR/Cas9
Abstrak: Cassava (Manihot esculenta) contain toxic quantities of cyanogenic glucoside. Reducing the potentially toxic cyanogenic glucoside levels in cassava could be achieved by gene editing through Clustered Regularly Interspaced Short Palindromic Repeats – CRISPR associated protein 9 (CRISPR/Cas9), a high- precision genome editing technique in plants. Two cassava UDP- glucosyltransferase (UGT) paralogs, UGT85K4 and UGT85K5, are the genes catalyzing the synthesis of cyanogenic glucosides in plants. The study aimed to construct the CRISPR/Cas9 recombinant vector carrying single guide RNA (sgRNA) to edit UGT85K4 and UGT85K5 genes, and transform sgRNA CRISPR/Cas9 construct into KU50 cassava calli. Five sgRNAs were designed, considering the on/off target efficiency score and GC content. Those sgRNAs were ligated into pRGEB32 plasmid and introduced to competent Escherichia coli DH5a. The PCR colony and DNA sequencing were performed to verify the sgRNA insert. The amplicons showed the expected band size of recombinant vector (180 bp). Based on sequencing results, only one sgRNA was successfully ligated into the plasmid that will be used to edit the UGT85K5 gene. The sgRNA CRISPR/Cas9 construct in pRGEB32 plasmid was successfully transformed into Agrobacterium tumefaciens and introduced into KU50 cassava calli through co-cultivation. The overgrowth of Agrobacterium occurred after the co-cultivation phase.
Keyword: CRISPR/Cas9, Cyanogenic glucoside, Gene editing, sgRNA