Judul: Buprenorphine and Fischer-344 Rats Post-operative Stress Levels: Impact on Serum Corticosterone Levels.
Abstrak: The use of laboratory animals in experiment often requires surgical operations. Post-operative condition often associated with pain and stress. Preemptive analgesia and post operative treatment with buprenorphine can reduce post operative pain and stress and prevent prolonged pain. Therefore, the aim of the present study was to test the hypothesis that pre operative treatment of buprenorphine reduces stress and suppresses the pre-operative serum corticosterone levels in Fischer 344 rats. A total of 14 adult male Fischer 344 (F344/Sca) rats permanently catheterized in artery carotis communis were used. During 94 hour post-operatively the blood sample was collected at specific time point using Accusampler®, an automated blood sampling. Corticosterone levels were quantified with ELISA method using sheep polyclonal antibody to corticosterone. Buprenorphine is effective as pre-emptive analgesia in F344 strain. However the dose is higher than what has been reported in Sprague Dawley strain. Buprenorphine 1.0 mg/kg significantly suppressed serum corticosterone levels immediately after rats regain consciousness. Rats in buprenorphine 1.0 mg/kg group were protected from post operative body weight loss and reduced water intake compare to control, nutella, and buprenorphine 0.4 mg/kg group. Quantification of fecal corticosteroid metabolites failed to detect stress in present study.
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Judul: Effects of Adrenaline Administration to Feed Intake Level of White Rats (Rattus norvegicus)
Abstrak: Adrenalin merupakan hormon katekolamin yang disekresi oleh medulla kelenjar adrenal. Hormon ini berfungsi untuk memfasilitasi perubahan fisiologis dalam menghadapi kondisi stres. Perubahan kondisi fisiologis yang disebabkan oleh adrenalin diantaranya adalah dilatasi bronkus, peningkatan tekanan darah, peningkatan kadar glukosa darah, penurunan motilitas dan kontraktilitas saluran pencernaan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian adrenalin terhadap tingkat konsumsi pakan pada tikus Rattus norvegicus. Penelitian ini menggunakan tikus sebanyak 24 ekor yang dibagi menjadi empat kelompok perlakuan, yaitu puasa 12 jam + injeksi adrenalin 0.6 mg/kg (PA), puasa 12 jam (P), injeksi adrenalin 0.6 mg/kg (A), dan kontrol (C). Perlakuan dilakukan selama tujuh hari dan pengukuran tingkat konsumsi pakan dilakukan tiap hari setelah 12 jam perlakuan. Uji One-way ANOVA dengan taraf kepercayaan 95% dilakukan untuk perbandingan data. Hasil penelitian menunjukkan bahwa injeksi adrenalin dapat mengurangi tingkat konsumsi pakan di hari awal perlakuan dan dapat meningkatkan tingkat konsumsi pakan setelah beberapa pengulangan walau masih berada dalam rentang normal., Adrenaline is a catecholamine hormone secreted by medulla of adrenal gland. Adrenaline is secreted to facilitate physiological changes when facing stressful conditions. The physiological changes caused by adrenaline are bronchial dilatation, increase of blood pressure, increase of blood glucose level and decrease of gastrointestinal tract motility and contractility. This research aimed to study the effects of adrenaline to feed intake level in Rattus norvegicus. This research used 24 rats and divided it into four treatments namely, 12 hours fasting + 0.6 mg/kg adrenaline injection (PA), 12 hours fasting (P), 0.6 mg/kg adrenaline injection (A) and control (C). The treatment was done in seven days and feed intake level was measured 12 hours after treatment each day. A one-way ANOVA test was performed using a 95% level of significance for comparison test. The result of this research showed that adrenaline injection was able to decrease feed intake level in the first day of treatment and increase feed intake after subsequent treatments although the increase was still within normal range.
Keyword: adrenaline, fasting, feed intake level, Rattus norvegicus

Judul: Formulasi Hamiltonian untuk Menggambarkan Gerak Gelombang Soliter Dimensi Tiga di Permukaan Laut
Abstrak: Gelombang permukaan adalah gelombang yang terjadi perbedaan rapat massa antara air dan udara. Salah satu gelombang permukaan yang ditinjau adalah gelombang soliter. Formulasi gelombang soliter dilakukan dengan menggunakan formulasi Hamiltonian.
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