Judul: Study of reproduction performance of female African catfish (Clarias gariepinus) with supplemented by Ascorbyl Phosphate magnesium as a source of vitamin C in diets and implanted with estradiol-17β
Abstrak: The experiment was conducted to determine the effect of dietary ascorbyl phospahate magnesium as a source of vitamin C and implanted with estradiol-17 β on the gonad maturation, egg and larva quality of African Catfish Clarias gariepinus. Fish were treated by various combinations of dietary dosage of ascorbyl phosphate magnesium (0, 600, 1200, and 1800 mg/ kg of feed) and estradiol-17β (00, 250, and 500μ g/kg). Two hundreds sixteen and eighteen pairs of broodstock fish were used for this experiment. Fish were fed with the experimental diets two times a day at satiation. The gonad somatic index, egg diameter, fecundity, hatching rate of the eggs, survival rate, and percentage of abnormal larvae were determined. Results of the experiment indicated that supplementation of ascorbyl phosphate magnesium and estradiol-17β stimulated gonad development and increased hatching rate, fecundity and survival rate, and reduced percentage of abnormal larvae. Combination of ascorbyl phosphate magnesium 1200 mg/kg feed and estradiol-17β 250μ g/kg gave the best reproductive performance.
Keyword: African catfish, Clarias gaviepinus, Ascoryl phosphate magnesium, Reproduction performance

Judul: The Effectiveness of Addition of  Vitamin C with Different Doses as an Antioxidant in Sperm  Cryopreservation of Catfish (Clarias gariepinus).
Abstrak: Salah satu masalah yang dihadapi dalam pembenihan ikan lele adalah siklus reproduksi yang tidak terjadi sepanjang tahun sehingga ketersediaan induk jantan yang matang gonad menjadi langka. Pada metode pemijahan buatan, induk jantan harus dikorbankan saat proses koleksi sperma karena tidak bisa dilakukan stripping, namun jumlah sperma yang dihasilkan cukup banyak. Metode kriopreservasi dapat menjadi alternatif agar sperma yang telah dikoleksi melalui pembedahan dapat disimpan dalam waktu yang lama dan digunakan saat dibutuhkan. Kriopreservasi memiliki efek negatif karena adanya pengaruh dari reactive oxygen species (ROS) yang menyebabkan penurunan kualitas spermatozoa. Alternatif yang dapat dilakukan yaitu menggunakan antioksidan berupa vitamin C. Penelitian ini bertujuan untuk mengevaluasi pengaruh penambahan vitamin C dengan dosis berbeda dalam krioprotektan terhadap performa sperma ikan lele pada proses reproduksi setelah kriopreservasi. Penelitian ini terdiri dari lima perlakuan dan tiga ulangan yaitu kontrol (sperma segar), DMSO 10%, DC 1 (vitamin C dosis 1 mg/mL), DC 2,5 (vitamin C dosis 2,5 mg/mL), dan DC 4 (vitamin C dosis 4 mg/mL). Kriopreservasi sperma dilakukan selama satu jam. Hasil menunjukkan motilitas, viabilitas, dan derajat pembuahan telur pada perlakuan vitamin C lebih rendah (P<0,05) dari kontrol. Derajat penetasan telur perlakuan kontrol dan DC 1 tidak berbeda nyata dan perlakuan DC 4 menghasilkan nilai terendah., One of the problems encountered in catfish hatchery is the reproductive cycle which does not occur throughout the year so that the availability of mature male gonad broodstock is scarce. In the artificial spawning method, the male parent must be sacrificed during the sperm collection process because stripping cannot be done, even though the amount of sperm produced is quite a lot. The cryopreservation method can be an alternative so that sperm that has been collected through surgery can be stored for a long time and used when needed. Cryopreservation has a negative effect due to the influence of reactive oxygen species (ROS) which causes a decrease in the quality of spermatozoa. An alternative that can be done is to use antioxidants in the form of vitamin C. This study aims to evaluate the effect of adding vitamin C with different doses in cryoprotectants on the performance of catfish sperm in the reproductive process after cryopreservation. This study consisted of five treatments and three replications namely control (fresh sperm), 10% DMSO, DC 1 (vitamin C dose of 1 mg/mL), DC 2,5 (vitamin C dose of 2,5 mg/mL), and DC 4 (vitamin C dose of 4 mg/mL). Sperm cryopreservation was carried out for one hour. The results showed motility, viability, and egg fertilization rate in the vitamin C treatment were lower (P<0,05) than the control. The hatching rate of the control and DC 1 treatment was not significantly different and the DC 4 treatment was the lowest value.
Keyword: Cryopreservation, fertility, motility, viability, vitamin C

Judul: Verifikasi hotspot dan identifikasi kebakaran vegetasi melalui deteksi pencilan data deret waktu NDVI
Abstrak: Fenomena kebakaran hutan merupakan peristiwa jamak di daerah kutub, subtropis dan tropis seperti Indonesia. Kejadian kebakaran dimonitor dengan satelit NOAA dan data disajikan sebagai sebaran hotspot. Data hotspot ditetapkan dari kanal termal dengan suhu ambang yang ditetapkan secara arbitrer oleh penyedia data, sehingga ketidakpastian hotspot sebagai indikator kejadian kebakaran relatif tinggi. Oleh karena itu diperlukan teknik untuk memverifkasi hotspot sebagai api aktif.
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