Judul: Hubungan Antara Viabilitas, Motilitas, dan Keutuhan Membran Plasma Spermatozoa Semen Beku Sapi Limousin
Abstrak: Limousine is a popular beef cattle among farmers. According to the Indonesian National Standard (SNI), the quality of frozen semen that is distributed and used to inseminate cattle must have a post-thawing motility of > 40% and an individual scoring > 2. This study aimed to test the quality of Limousine frozen semen with different parameters, namely the percentage of spermatozoa motility, live-dead spermatozoa (viability; indicator of viable sperm), the percentage of plasma membrane integrity of spermatozoa (MI) and to find the relationship between these three parameters. The motility was assessed subjectively using three evaluators, the viability was assessed with eosin nigrosin staining and membrane integrity (MI) was tested using hypo osmotic swelling (HOS) test. All was observed under the microscope and data were analyzed using the Pearson correlation SPSS 16 for windows. Data showed that the frozen semen of all four individuals were still in good condition and the post thawing motility of all bulls qualified the SNI for frozen semen of bulls. There was a positive correlation between motility and viability, motility and MI as well as viability and membrane integrity.
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Judul: Longivitas dan Viabilitas Spermatozoa Sapi Friesian Holstein, Simmental, dan Brahman dalam Semen Beku Menggunakan Pengencer Skim.
Abstrak: Indonesia had two artificial insemination centre and more than 17 regional artificial insemination centre. Before distributed to farmers, the quality of frozen semen has to be laboratory tested periodically. According to the Indonesian National Standard the parameters assessed in the laboratory are sperm motility and individual scoring. The aim of this study was to test the sperm motility, viability and longevity of frozen semen from three cow breeds which are FH, Simmental and Brahman produced by one of the regional artificial insemination centers and to fine out the relationship between sperm motility and its viability among breed. Results showed that post thawing motility of the FH bull was 50.00±0.00% which was significantly higher than Simmental 43.50±2.42% or Brahman 43.33±2.50% bull. A decrease in motility and viability showed an association motility with the percentage of live spermatozoa. They were no differences in sperm longevity among breeds. The motility of all samples decreased and reached 0% motility in 5 hours. The decrease in sperm motility and viability is caused by the decrease in energy sources and the accumulation of toxic waste of metabolized diluents. In conclusion, the FH bull had the best post thawing motility and viability compared to Simmental and Brahman bull. Based on the Indonesian National Standard all sample could be used for insemination.
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Judul: Zonasi bahaya serangan rayap tanah terhadap bangunan gedung di Jakarta Selatan
Abstrak: Jakarta Selatan merupakan salah satu kota administrasi di Provinsi DKI Jakarta dengan pertumbuhan infrastruktur yang sangat tinggi. Sejalan dengan hal itu, frekuensi serangan rayap terhadap bangunan gedung di kota tersebut juga cukup tinggi. Suatu penelitian telah dilakukan untuk menyusun zonasi kelas bahaya serangan rayap tanah terhadap bangunan gedung berdasarkan jenis rayap, karateristik tanah, dan cuaca. Dari 10 kecamatan di Jakarta Selatan, dipilih secara acak lima Kecamatan Contoh. Di masing- masing Kecamatan Contoh, kemudian dipilih tiga Kelurahan Contoh sebagai lokasi pemasangan kayu umpan untuk pengumpulan spesies rayap tanah di lokasi tersebut. Kayu umpan yang terbuat dari kayu pinus (2x2x40 cm, KA= 18+ 2%) dibenamkan secara vertical ke dalam tanah di masing-masing Kelurahan Contoh. Rayap tanah yang ditemukan diidentifikasi menggunakan kunci determinasi rayap dari Ahmad (1958). Dari masing-masing Kecamatan Contoh diambil contoh tanah untuk dianalisis kandungan C- organik, pH, kadar air dan rasio pasir-liatnya. Adapun data cuaca diliput dari Badan Meteorologi Klimatologi dan Geofisika Jakarta Selatan. Hasil penelitian menunjukkan bahwa sebagian besar wilayah Jakarta Selatan (80%) tergolong Kelas Bahaya I (wilayah dengan ancaman serangan tinggi), sedangkan sisanya tergolong Kelas Bahaya II (wilayah dengan ancaman serangan sedang). Keberadaan rayap Coptotermes sp di Kecamatan Kebayoran Baru, Pasar Minggu, Pesanggrahan dan Mampang Prapatan ini patut menjadi perhatian, mengingat tingkat serangan dari rayap ini sangat tinggi.
Keyword: South Jakarta, Species of subterranean termites, Hazard class, Termites attack, Forestry, Macrotermes gilus, Coptermes, Microtermes pallidus, Termitidae