Judul: Analisis optimalisasi perencanaan usahatani di unit pemukiman transmigrasi hutan tanaman industri suban jeriji SP3, Muara Enim, Sumatera Selatan
Abstrak: Usaha yang dilakukan pemerintah untuk menggulangi masalah kependudukan dan mengingkatkan taraf hidup masyarakat anatara lain adalah program transmigrasi. Salah satu program transmigrasi di mana pihak swasta terlibat sebagai pengelola, yang dilaksanakan pada tahun 1992, adalah transmigrasi Pola HTI terpadu di Suban Jeriji, Kabupaten Muara Enim, Propinsi Sumatera Selatan. Transmigrasi ini dikelola oleh PT. Musi Hutan Persada (PT. MHP) dan dilaksanakan guna memenuhi kebutuhan tenaga kerja di areal HTI.
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Judul: Optimasi luas lahan unit HTI-Trans ditinjau dari segi penyiapan lapangan kerja, ekonomi dan finansial : Studi kasus di PT. Rimba Seraya Utama, Riau)
Abstrak: Meningkatnya kebutuhan akan produk hasil hutan khusus- nya yang berupa kayu, menyebabkan makin meningkatnya eks- ploitasi terhadap hutan. Sementara potensi hutan alam sangat terbatas. Dalam rangka memenuhi kebutuhan tersebut Departemen Kehutanan menetapkan program pembangunan Hutan Tanaman Industri (HTI) dalam skala besar. Kendala yang dihadapi dalam pelaksanaan program HTI adalah kurangnya tenaga kerja, karena pembangunan HTI umumnya dilaksanakan di luar pulau Jawa dengan kepadatan penduduk yang relatif ren- dah. Sementara itu distribusi penduduk yang tidak merata menyebabkan laju pembangunan yang tidak seimbang. tah dalam hal ini melakukan program transmigrasi. Pemerin- Kendala yang dihadapi adalah dalam rangka penyediaan pemukiman mau- pun lahan usaha bagi transmigran. Guna mengatasi permasalahan tersebut Departemen Kehu- tanan dan Departemen Transmigrasi dan PPH melakukan kerjasa- ma dalam program HTI-trans. Kerjasama yang saling mengun- tungkan antara pengusaha dan transmigran. Perusahaan menda- patkan tenaga kerja secara kontinu sedangkan transmigran mendapatkan pekerjaan yang tetap dan lahan usaha pada proyek HTI. Program HTI-Trans merupakan 2 kepentingan yang dipadu- kan yaitu kepentingan perusahaan dan transmigran. Untuk itu penting terciptanya pengelolaan HTI dengan pemukiman trans- migran yang harmanis, bekesinambungan, saling menguntungkan dan berwawasan lingkungan. Pengaturan kelestarian hasil dapat dilakukan melalui pengaturan luas. Luas optimal akam memberikan keuntungan yang maksimal baik bagi perusahaan maupun bagi kepentingan transmigran...dst
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Judul: DNA Extraction and Characterization of Salmonella‟s Lytic Bacteriophage Genome
Abstrak: Bacteriophages are ubiquitous in nature and are known to proliferate wherever their bacterial hosts exist. Their virion particles can exist independently outside the host, however all phages are need their host to propagate. Several phages are highly specific to host cell surface receptors and any slight changes in structure results in little or no interaction between the phage and its host. Salmonella‟s phage isolated from sewage water where their bacteria exist. Three isolated of phages (phages 15, 19 and 38) were used in this research that concentrated using three types of methods. PEG/ NaCl method was firstly used to concentrate the phages based on their molecular weight to precipitate the phage particles during centrifugation. The second method was Scraping Plaque method. In this method, overlay plaques were scraped and collected in SM buffer to get phage concentrates. Enrichment method was the last method used to concentrate the phages. Phage genome isolation was done using two methods i.e. EDTA/SDS and Proteinase K methods. The first method was done using DNase I to eliminate bacterial DNA followed by SDS/EDTA treatment to release phages DNA and phenol-chloroform-isoamyl alcohol to extract the DNA. DNA was collected by isopropanol extraction. While in Proteinase K method, bacterial DNA was eliminated by DNase I and Proteinase K/SDS was used to degrade phages capsids and ethanol for DNA precipitation. The result showed that PEG/NaCl method resulted the highest phage concentration for phage 15 and phage 19, while scrapping method was the highest for phage 38. The quantity and purity of extracted DNA were low for PEG/NaCl and Scraping methods, while Enrichment method was higher indicated higher purity. Run on agarose gel electrophoresis of extracted DNA confirmed that Enrichment method results were the best while scraping and PEG/NaCl method was appeared smear background. Based on DNA genome size determination, the bacteriphages of Salmonella sp were comparable with other study of Salmonella phages. Phage P15 with genome size of 39,5 kb was close to phage epsilon15. Phage P19 with genome size of 41 kb was close to phage ST160. And phage P38 with genome size of 48,5 kb was close to phage Gifsy-1.
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