Judul: Impact of Instagram Usage Intensity on Body Dissatisfaction, Eating Behaviour, and Nutritional Status in Adolescents
Abstrak: Media sosial yang menampilkan figur tubuh ideal menyebabkan remaja khususnya perempuan membandingkan bentuk tubuhnya dengan standar sosial. Hal tersebut menyebabkan terciptanya ketidakpuasan tubuh dan mendorong remaja untuk melakukan berbagai upaya seperti diet berlebih yang dapat memengaruhi citra tubuh, perilaku makan, dan status gizi. Penelitian ini bertujuan untuk menganalisis pengaruh intensitas penggunaan instagram terhadap body dissatisfaction, perilaku makan, dan status gizi remaja kota dan kabupaten. Desain penelitian menggunakan cross sectional study yang terdiri dari 50 subjek kota dan 50 subjek kabupaten yang dipilih secara purposive sampling. Pengumpulan data dilakukan melalui wawancara dan self-administered secara luring. Sebagian besar subjek masuk kedalam kategori light user pada penggunaan instagram (69%), memiliki persepsi body dissatisfaction (57%), tergolong dalam tipe perilaku makan external eating (68%), beresiko mengalami gangguan makan (12%), dan memiliki status gizi normal (69%). Tidak terdapat pengaruh signifikan antara intensitas penggunaan instagram terhadap body dissatisfaction, perilaku makan dan status gizi (p>0,05). Terdapat pengaruh signifikan antara body dissatisfaction terhadap perilaku makan, penyimpangan perilaku makan, dan status gizi pada kedua kelompok subjek (p<0,05). Terdapat pengaruh perilaku makan terhadap status gizi pada kedua kelompok subjek (p<0,05). Dapat disimpulkan bahwa instagram tidak secara langsung mempengaruhi body dissatisfaction pada remaja, namun terdapat faktor penyebab lain yang berkontribusi lebih besar., Social media that displays ideal body figures causes adolescents (especially women) to compare their body shape with social standards. This phenomenon can cause body dissatisfaction and encourages adolescents to make various efforts, such as excessive dieting, which can affect body image, eating behavior, and nutritional status. This study aims to analyze the effect of the intensity of Instagram use on body dissatisfaction, eating behavior, and nutritional status of city and district adolescents. The research design used a cross-sectional study of 50 city subjects and 50 regency subjects selected by purposive sampling. Data was collected through interviews and offline self-administered. Most of the subjects fall into the light user category when using Instagram (69%), have a perception of body dissatisfaction (57%), belong to the external eating behavior type (68%), are at risk of experiencing eating disorders (12%), and have normal nutritional status (69%). There was no significant effect between the intensity of using Instagram on body dissatisfaction, eating behavior, and nutritional status (p>0,05). There was a significant effect of body dissatisfaction on eating behavior, deviant eating behavior, and nutritional status in both subject groups (p<0,05). There is an effect of eating behavior on nutritional status in both groups of subjects (p<0,05). The conclusion is that instagram does not directly affect adolescent body dissatisfaction, but other factors contribute more.
Keyword: adolescents, body dissatisfaction, eating behaviour, eating disorder, instagram, nutritional status, perilaku makan, penyimpangan perilaku makan, remaja, status gizi

Judul: The Correlation between Body Image, Intensity of Social Media Use, Diet Quality, Physical Activity, and Nutritional Status in Adolescents
Abstrak: Perubahan biologis pada fase remaja dan banyaknya paparan terkait standar ideal di media sosial dapat memicu citra tubuh yang negatif pada remaja. Hal tersebut menyebabkan adanya ketidakpuasan tubuh dan mendorong remaja untuk melakukan berbagai upaya seperti diet berlebih dan aktivitas fisik berlebih sehingga dapat mempengaruhi status gizinya. Tujuan penelitian ini adalah untuk menganalisis intensitas penggunaan media sosial, body image, kualitas diet, aktivitas fisik, dan status gizi pada remaja di SMAN 1 Bogor. Desain penelitian ini adalah cross-sectional dengan jumlah subjek 87 siswa yang dipilih secara purposive sampling. Pengumpulan data dilakukan melalui pengukuran langsung, wawancara, dan pengisian kuesioner. Sebagian besar subjek memiliki intensitas penggunaan media sosial pada kategori heavy user (53%), memiliki body image positive (60%), memiliki aktivitas fisik sedang (41%) dan status gizi baik (70%). Rata-rata skor DQI-A subjek berada pada nilai 40,7% dan dikategorikan kurang baik. Terdapat hubungan yang signifikan antara intensitas penggunaan media sosial dengan body image (p<0,05) dan terdapat hubungan yang signifikan antara body image dengan status gizi (p<0,05). Tidak terdapat hubungan yang signifikan antara body image dengan kualitas diet serta aktivitas fisik. Tidak terdapat hubungan yang signifikan antara aktivitas fisik serta kualitas diet dengan status gizi (p>0,05)., Biological changes during adolescence and extensive exposure to ideal standards on social media can trigger negative body image among adolescents. This leads to body dissatisfaction and encourages adolescents to make various efforts, such as excessive dieting and physical activity, which can affect their nutritional status. This study aims to analyze the intensity of social media use, body image, diet quality, physical activity, and nutritional status among adolescents at SMAN 1 Bogor. The design of this study is cross-sectional, with a total of 87 students selected through purposive sampling. Data collection was conducted through direct measurements, interviews, and questionnaires. Most subjects have the intensity of social media use in the heavy user category (53%), have a positive body image (60%), have moderate physical activity (41%), and good nutritional status (70%). The average DQI-A score of the subjects was 40.7% and categorized as poor. There was a significant correlation between the intensity of social media use and body image (p<0.05) and there was a significant correlation between body image and nutritional status (p<0.05). There was no significant correlation between body image with diet quality and physical activity. There was no significant correlation between physical activity and diet quality with nutritional status (p>0.05).
Keyword: body image, nutritional status, Social Media, adolescent

Judul: Isolasi Konstruksi dan Rekombinasi Gen Pengkode Reverse Transcriptase asal Simian Retrovirus-2 (SRV-2)
Abstrak: The availability of reverse transcriptase enzym as a valuable component for Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique has been providing by foreign companies. Therefore, it needs more cost and time to provide it. This study is an early step to produce in house reverse transcriptase rekombinant enzym from SRV-2. The purpose of this study was isolated, constructed, and rekombined SRV-2 reverse transcriptase gene to entry clone and destination vector. Isolation was done by PCR amplification technique using three pairs of primers, which are RT I (3282-3683), RTII (3261-3900), and RTIII (3169-4133). Construction and recombination in this study used Gateway method from Invitrogen. SRV-2 reverse transcriptase gene was isolated in this study with A-549 cell inoculated SRV-2 as source of virus. This gene amplify by PCR technique and resulted specific band for RTI 401 bp, RTII 639 bp, and RTIII 964 bp. Analysis of pENTR/SD/TOPO and pDEST17 indicated SRV-2 reverse transcriptase gene code has already inserted to this plasmid. This result indicated SRV-2 reverse transcriptase gene have isolated, constructed, and recombined to entry clone and destination vector.
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