Judul: Optimisasi Ekstraksi dan Formulasi Temu Putih (Curcuma zedoaria) dan Temu Hitam (Curcuma aeruginosa) sebagai Antikanker serta Analisis Sidik Jari Formula
Abstrak: Temu putih dan temu hitam diketahui berpotensi sebagai antikanker. Penelitian ini bertujuan mencari kondisi optimum ekstraksi sonikasi dan formulasi kedua rimpang tersebut dengan meragamkan polaritas pelarut etanol:air dan waktu ekstraksi antara 10 sampai 30 menit. Penelitian dirancang menggunakan rancangan D-Optimal. Kondisi optimum ditentukan berdasarkan uji toksisitas terhadap Artemia salina dan diperoleh saat digunakan pelarut etanol 96% selama 10 menit. Kedua ekstrak tunggal diformulasi menjadi 10 formula dan diuji toksisitasnya terhadap A. salina. Formula F6 dan F9 dengan nisbah temu putih, temu hitam, dan pati masing-masing sebanyak 1:1:1 dan 2:1:0 memiliki nilai LC50 terendah, yaitu berturut-turut 71.31 dan 61.81 ppm. Formula tradisional (F11) diekstraksi dengan kondisi optimum dari kedua ekstrak tunggal dan memiliki nilai LC50 sebesar 58.63 ppm. Hasil uji toksisitas terhadap sel kanker A549 menunjukkan formula F11 memiliki persen inhibisi sebesar 48.16% pada konsentrasi 5 ppm, inhibisi F9 pada konsentrasi 250 ppm di bawah 50% namun telah merusak morfologi sel kanker A549, sedangkan F6 tidak menghambat proliferasi sel dan tidak merusak morfologinya. Analisis sidik jari F11 dengan beberapa fase gerak menghasilkan 1 hingga 7 pita.
Keyword: Curcuma zedoaria, Curcuma aeruginosa, Optimization, Artemia salina, A549 cell

Judul: Efek Pemberian Ekstrak Etanol Temu Ireng (Curcuma Aeruginosa Roxb.) pada Proliferasi Sel Lestari Tumor THP1 dan A549 Secara In Vitro.
Abstrak: The use of plant extracts is one of alternative ways to heal tumor diseases. The purpose of this research is to study the effect of ethanol extract of temu ireng (Curcuma aeruginosa Roxb.) on proliferation of THP1 and A549 cell lines. The extract concentration used were 0 ppm (as a negative control), 12.5 ppm, 25 ppm; 37.5 ppm and 50 ppm, doxorubicin as a positive control, and the treatment were in three replications. All data were analyzed statistically by ANOVA and Duncan test. The results showed that inhibition activity of temu ireng ethanol extract on THP1 and A549 cell line proliferation were significantly different (p<0.05) than negative control. Inhibition activity were as higher as treatment dose and significantly different (p<0.05) between dose, except 25 ppm and 37.5 ppm dose on THP1 cell line. The highest inhibition was achieved at 50 ppm dose as 85,8% for THP1 cell line and 73.3% for A549 cell line. Based on this results, we concluded that temu ireng ethanol extract has a poliferation inhibition activity on both cell lines and has a potential substance to be develop as antitumor.
Keyword: A549, curcuma aeruginosa Roxb, inhibition activity, THP1

Judul: The Macroeconomic effects of goverment expenditure in ASEAN plus Three
Abstrak: Fiscal policy is one of the goverment policy to increase growth in a reasonable level. The impact of fiscal policy shock has different effect on each country around the world. The effect of goverment spending shocks led to increase growth in USA but decrease growth in Germany, as reflected by the decline in private investment. This study aims to analize the effect of goverment expenditure in ASEAN plus Three, by using vector error correction model (VECM), impulse respone function (IRF), forecasting error variance decomposition (FEVD) and pass-through effect.
Keyword: