Judul: Analisis Durasi Pengoperasian Micro Bubble Terhadap Kualitas Air Limpasan Green Roof Ekstensif
Abstrak: Green roof menjadi salah satu alternatif metode pemanenan air hujan (rainwater harvesting) seiring dengan berkurangnya daerah resapan air. Penambahan nutrisi–nutrisi penunjang pertumbuhan tanaman membuat air limpasan green roof menjadi turun kualitasnya. Parameter kekeruhan, TDS, DHL dan TSS masih belum memenuhi baku mutu untuk dapat dimanfaatkan sebagai air baku. Micro bubble menjadi salah satu metode alternatif pengolahan air limbah melalui proses aerasi yang dapat mempercepat waktu biodegradasi dua kali lipat lebih cepat. Perlakuan aerasi menggunakan micro bubble dilakukan selama 30, 45, 60 dan 75 menit. Baku mutu yang digunakan mengacu pada Permenkes No. 2 Tahun 2023 dan PP No. 22 Tahun 2021 Lampiran VI. Penentuan status mutu air menggunakan metode STORET. Pengolahan air limpasan green roof menggunakan micro bubble mengurangi beberapa parameter pencemar air dan telah memenuhi baku mutu pada parameter temperatur, TDS, pH, DO dan amonia. Nilai efisiensi penyisihan mencapai 75,42% pada aerasi 75 menit ditunjukkan dengan perubahan status mutu air limpasan green roof dengan kelas B (tercemar ringan) berdasarkan US-EPA., Green roof is one of the alternative methods of rainwater harvesting along with the reduction of water catchment areas. The addition of nutrients to support plant growth makes green roof runoff water deteriorate in quality. Turbidity, TDS, DHL and TSS parameters still do not meet the quality standards to be used as raw water. Micro bubble is an alternative method of wastewater treatment through an aeration process that can accelerate biodegradation time twice as fast. Aeration treatment using micro bubble is carried out for 30, 45, 60 and 75 minutes. The quality standard used refers to Permenkes No. 2 Tahun 2023 and PP No. 22 Tahun 2021 Lampiran VI. Determination of water quality status using the STORET method. Treatment of green roof runoff water using micro bubbles reduces several water pollutant parameters and has met the quality standards on temperature, TDS, pH, DO and ammonia parameters. The removal efficiency value reached 75,42% at 75 minutes of aeration is indicated by a change in the quality status of green roof runoff water with class B (lightly polluted) based on US-EPA
Keyword: aeration, micro bubble, water quality, extensive green roof

Judul: Analisis Kualitas Air Hujan dan Air Limpasan melalui Media Pasir dan Zeolit pada Green Roof.
Abstrak: Berkurangnya ruang terbuka hijau (RTH) dapat mengurangi wilayah resapan air sehingga cadangan air tanah berkurang. Sumber air bersih lain yang dapat dimanfaatkan adalah dengan pemanenan air hujan. Green roof dapat digunakan sebagai daerah tangkapan air hujan dalam rangka menambah ruang terbuka hijau serta filtrasi sederhana air hujan oleh mediumnya. Oleh karena itu, perlu dilakukan pengujian kualitas air hujan maupun air limpasan dari green roof. Tujuan penelitian adalah menguji dan menganalisis kualitas air hujan dan air limpasan green roof yang menggunakan media pasir dan zeolit berdasarkan baku mutu yang ditetapkan Permenkes No. 492 tahun 2010. Analisis status kualitas air dilakukan dengan metode STORET dalam KepmenLH No. 115 tahun 2003. Hasil penelitian menunjukkan kualitas air hujan memiliki mutu cemar ringan, sementara air limpasan green roof memiliki mutu cemar sedang hingga cemar berat.
Keyword: air hujan, air limpasan, green roof, kualitas air, pasir, zeolit

Judul: Mitochondrial DNA Gene Organization and Phylogenetic Relationship of Naja siamensis
Abstrak: Naja siamensis is one of Asiatic cobra in the Family of Elapidae, known as a cryptic snake. It has many morphological variations that lead to misidentification. Misidentification can cause the wrong concept in developing antivenom types due to high snakebite cases occur in Thailand. Thus, an accurate species identification using a molecular approach is crucially required. Mitochondrial DNA has been used widely in snake molecular studies. These studies were aimed to determine the arrangement of the N. siamensis partial mitochondrial genes using reptile-oriented primers and to reveal the phylogenetic relationship among other species of Asian cobra. DNA extraction was carried out based on the standard DNA salting-out protocol and was conducted by bioinformatics analysis using BLASTn and MEGA 6 software. Gene annotation was performed against N. kaouthia complete genome. A total of 3121 bp of N. siamensis mtDNA genes were sequenced consisting of partial of six regions: 16S rRNA, ND1, tRNA-Ile, CRII region, tRNA-Leu, tRNA-Gln, and COI genes. The mtDNA gene organization of N. siamensis was predicted to has a similar pattern with the common Elapidae mtDNA gene organization. This study showed that there is a monophyletic form in Naja genus and N. kaouthia is the closest species with N. siamensis based on partial 16S rRNA and ND1 gene.
Keyword: 16S rRNA, Elapidae, ND1, phylogenetic relationship