Judul: Decomposition of Agathis dammara (Lamb.) Leaves,  Soil Fauna Abundance, and Soil Characteristics in Gunung Walat University  Forest
Abstrak: Serasah adalah sumber nutrisi bagi unsur biotik di sekitarnya. Fauna tanah berperan dalam dekomposisi serasah, menguraikan unsur hara, dan memengaruhi siklus nutrisi. Penelitian ini bertujuan menganalisis hubungan antara faktor edafis dan klimatis terhadap laju dekomposisi daun serta mengidentifikasi fauna tanah di bawah tegakan Agathis dammara (Lamb.). Pengambilan sampel dilakukan pada petak contoh 20 m x 20 m secara acak dengan sub-plot systematic sampling 0,5 m x 0,5 m. Hasil analisis menunjukkan laju dekomposisi pada minggu ke 4, 8, dan 12 secara berturut-turut sebesar 0,92 g/minggu, 1,51 g/minggu, dan 1,29 g/minggu. Korelasi antara laju dekomposisi dengan faktor edafis seperti pH, suhu, kelembapan, respirasi, bahan organik, dan c-organik menunjukkan korelasi positif. Faktor klimatis juga berpengaruh: kelembapan udara dan suhu udara memiliki korelasi positif, sedangkan intensitas cahaya menunjukkan nilai negatif. Indeks keanekaragaman fauna tanah berkisar antara 0,30-1,90; nilai kemerataan berkisar antara 0,15-0,82; dan nilai kekayaan berkisar antara 0,63-2,10; yang menunjukkan indeks keanekaragaman yang tergolong rendah hingga sedang.
Keyword: soil fauna

Judul: Decomposition Rate and Site Characteristics in the Agroforestry System at Mount Kendeng Resort, Mount Halimun Salak National Park.
Abstrak: Leaf litter has a positive influence on the physical and chemical properties of soil through the provision of organic matter on the soil surface. This study aimed to measure the decomposition rate of leaf litter, and analyze the relationship between site characteristics and environmental conditions on the decomposition rate in agroforestry systems areas. Vegetation analysis was carried out on 50 m x 50 m sample plots totalling 14 plots. In addition, decomposition rate analysis, site characteristics, climate, and correlation tests were carried out. The dominant tree species in agroforestry systems areas was Agathis dammara. The highest and lowest decomposition rates in agroforestry systems areas at Gunung Kendeng Resort were 0.57 g/day and 0.33 g/day, respectively. The correlation between decomposition rate and edaphic factors that had a positive correlation were pH, soil organic matter, organic carbon, and porosity, while soil temperature, soil respiration, and bulk density had a negative correlation. The correlation between decomposition rate and climatic factors that had a positive correlation was moisture, while air temperature and light intensity had a negative correlation., Serasah memiliki pengaruh yang positif terhadap sifat fisik dan kimia tanah melalui penyediaan bahan organik di permukaan tanah. Penelitian ini bertujuan untuk mengukur laju dekomposisi serasah daun, dan menganalisis hubungan karakteristik tapak dan kondisi lingkungan terhadap laju dekomposisi pada areal sistem agroforestri. Analisis vegetasi dilakukan pada plot contoh 50 m×50 m sebanyak 14 plot. Selain itu, dilakukan analisis laju dekomposisi, karakteristik tapak, dan klimatis, serta uji korelasi. Jenis pohon yang mendominasi pada areal sistem agroforestri adalah Agathis dammara. Laju dekomposisi tertinggi dan terendah pada areal dengan sistem agroforestri di Resort Gunung Kendeng secara berturut-turut sebesar 0,57 g/hari dan 0,33 g/hari. Hubungan korelasi laju dekomposisi dengan faktor edafis yang memiliki korelasi positif adalah pH, bahan organik tanah, C-Organik, dan porositas, sedangkan suhu tanah, respirasi tanah, dan bulk density memiliki korelasi negatif. Hubungan korelasi laju dekomposisi dengan faktor klimatis yang memiliki korelasi positif adalah kelembapan, sedangkan suhu udara dan intensitas cahaya memiliki korelasi negatif.
Keyword: agroforestri, dekomposisi, karakteristik edafis, karakteristik klimatis, serasah

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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