Judul: Transplantasi sel testikular ikan neon tetra Paracheirodon innesi pada benih ikan mas umur berbeda
Abstrak: Ikan neon tetra Paracheirodon innesi merupakan ikan hias yang memiliki nilai ekspor yang tinggi. Namun demikian, tingkat produksinya masih relatif rendah karena fekunditas ikan neon tetra yang sedikit (sekitar 180 telur/induk). Teknologi transplantasi sel testikular ikan neon tetra (ikan donor) ke ikan mas yang memiliki fekunditas telur yang banyak dan diharapkan mampu mengatasi ketersediaan benih ikan neon tetra. Penelitian ini bertujuan untuk menentukan umur optimum benih ikan mas (calon ikan semang) yang mampu menerima sel target dengan baik dan memiliki keberhasilan kolonisasi yang tinggi. Testis ikan neon tetra didisosiasi menggunakan larutan tripsin 0,5%. Sel testikular diwarnai dengan PKH-26, kemudian ditransplantasikan ke rongga peritoneal benih ikan mas umur 7, 10 dan 14 hari setelah menetas. Hasil penelitian menunjukkan bahwa tingkat kelangsungan hidup ikan mas perlakuan transplantasi umur 7 hari (31,25%) lebih rendah dibandingkan dengan perlakuan transplantasi umur 10 hari (37,50%) dan 14 hari (56,25%). Persentase ikan terkolonisasi sel donor pada hari ke-21 pascatranplantasi pada benih umur 7 dan 10 hari adalah sama (80%), sedangkan transplantasi benih umur 14 hari sebesar 60%. Berdasarkan keberhasilan transplantasi secara kumulatif (tingkat kelangsungan hidup dan kolonisasi), transplantasi pada benih umur 14 hari (33,75%) menunjukkan hasil lebih tinggi dibandingkan dengan perlakuan transplantasi pada benih umur 7 hari (25,00%) dan benih umur 10 hari (30,00%). Dengan demikian, transplantasi sel testikular ikan neon tetra pada benih ikan mas telah berhasil dilakukan, dan umur optimum benih ikan mas adalah 14 hari setelah menetas.
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Judul: Transplantasi sel testikular ikan gurame Osphronemus gouramy pada ikan nila Oreochromis niloticus umur 1-4 hari
Abstrak: Giant gouramy is one of the targeted farmed species to increase its production level in the Indonesian Ministry of Maritime Affairs and Fisheries Program 2010-2014. Its production seemed slow because of the relatively long reproductive cycle. Transplantation of giant gouramy testicular cells to tilapia can be done in the hope that the surrogate broodstock (tilapia) will be able to give birth giant gouramy so the production cycle can be accelerated. In this research, transplantation of giant gouramy testicular cell (donor) in the peritoneal cavity of newly hatched tilapia (recipient) was performed. Giant gouramy testicular cells that contain spermatogonial cells obtained by dissociation of the gonad using PBS with 0.5% trypsin and then injected into tilapia with different ages. The results showed that SR (survival rate) of 1-2 days old injected larvae (A; 89.34%) were lower compared to that of 3-4 days old (B; 98.96%). Furthermore, the success rate of donor cells colonization in the recipient gonad at 2 months after injection in treatment A (6 of 6 recipients; 100%) was higher than that of treatment B (3 of 4 recipients; 75%). The results of this study indicated that transplantation using 1-2 days old larvae gave highest colonization and comparable level of SR. Thus, this technique has the potential both to manipulate gamet production of economically important fish species that relatively difficult to reproduction (commercial) and reproduce the endangered fish (as conservation).
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Judul: Penyelesaian Numerik Persamaan Schrödinger untuk Molekul BaO dan SrO Menggunakan Model Potensial Morse
Abstrak: Morse potential is a potential model used to describe the behavior of a molecular vibration between atoms. This study aimed to obtain the value of the dissociation energy, the energy eigenvalues of each energy level, and describe the shape of the wave function of the molecule BaO and SrO on the vibrational state. Dissociation energy is calculated by adding the experimental dissociation energy and the ground state energy eigenvalues. Calculating the energy eigenvalues of the energy levels 0 to 4 using shooting method integrated with Numerov and Runge-Kutta method. Energy eigenvalues of each levels that calculated numerically having accuracy of 100% compared with the calculated analytically. The profil of wave functions accurately show the corresponding states and their normalized wavefunctions.
Keyword: SrO, Morse potential, energy eigenvalues, dissociation energy, BaO