Judul: Kualitas Udara Ambien Berdasarkan Kondisi Atmosfer di Luar dan di Dalam Hutan Kota Monumen Nasional Jakarta
Abstrak: Hutan kota memiliki berbagai manfaat, salah satunya sebagai lokasi olahraga karena dianggap memiliki kualitas udara yang baik. Namun, kualitas udara dipengaruhi oleh berbagai faktor sehingga kualitas udara yang baik di dalam hutan kota tidak mutlak terjadi. Monumen Nasional dipilih sebagai lokasi penelitian karena terletak di pusat kepadatan lalu lintas Jakarta. Penelitian ini bertujuan untuk menganalisis kualitas udara ambien untuk parameter NO2, SO2, CO, dan Pb di luar dan di dalam hutan kota Monumen Nasional Jakarta. Sampel udara diambil pada pukul 05.00-06.00 WIB dan 09.00-10.00 WIB di jalan dan pukul 06.51-07.51 WIB dan 10.31-11.31 di hutan kota. Data pendukung berupa volume kendaraan, faktor meteorologi (suhu udara, kelembapan udara, arah dan kecepatan angin), vegetasi (komposisi jenis, stratifikasi tajuk, dan leaf area index), dan persepsi masyarakat. Konsentrasi polutan sebelum pukul 09.00 WIB lebih tinggi dibandingkan konsentrasi polutan setelah pukul 09.00 WIB. Konsentrasi polutan sebelum pukul 09.00 WIB lebih tinggi di luar hutan kota, sedangkan konsentrasi polutan setelah pukul 09.00 WIB lebih tinggi di dalam hutan kota. Namun, konsentrasi polutan udara di kedua titik lokasi masih di bawah baku mutu udara ambien sehingga masih aman untuk beraktivitas.
Keyword: hutan kota, jalan, kualitas udara ambien, olahraga

Judul: Pengujian Kualitas Udara Ambien Jalan Raya Kota Bekasi.
Abstrak: Air polution in Bekasi City is caused by 70% of transportation sector, 20% of industry sector, and 10 % domestic sector. Thus, in order to reduce air polution and improve the ambient air quality in Bekasi city , it needs to reduce air pollution produced either from tranportation or industrial sector. Based on the results of ambient air quality test along the roadside of Bekasi City during three periods in the year 2011, it showed that there are 2 parameters obtained which relatively increase, they are the TSP / dust and CO. For almost all parameters of CO along the observation points increased to the limit of specified quality standards. Parameters of TSP has highly increased throughout the year 2011. Almost TSP point exceed threshold quality standard (TQS). Based on the secondary data of CO concentration in 2010, the CO concentration is high in the morning and afternoon, and lower during the day and night. Ambient air quality in Bekasi city from 2009 to 2011 has decreased with increasing amount of tested pollutant concentrations, especially on the concentration of CO and TSP which have inclining done
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Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable