Judul: Konflik dan Persepsi Masyarakat Sekitar Kawasan Hutan Gunung Sawal terhadap Macan Tutul Jawa (Panthera pardus melas Cuvier 1809).
Abstrak: Konflik dapat menjadi masalah dalam upaya konservasi satwa liar, salah satunya upaya konservasi macan tutul jawa. Tujuan penelitian ini adalah mengidentifikasi faktor penyebab konflik antara manusia-macan tutul jawa dan tingkat persepsi masyarakat terhadap macan tutul jawa di Gunung Sawal. Selama juni 2018 hingga Maret 2020 diperoleh informasi 10 kasus konflik. Faktor utama yang menjadi penyebab konflik antara masyarakat dengan macan tutul jawa di sekitar kawasan hutan Gunung Sawal yaitu Suaka Margasatwa Gunung Sawal dinilai tidak mampu menampung seluruh individu macan tutul jawa. Skor persepsi masyarakat terhadap keberadaan kawasan hutan Suaka Margasatwa Gunung Sawal dan keberadaan macan tutul jawa serta konflik, secara berturut-turut 4.26, 3.74 dan 3.51. Hal ini menunjukkan tingkat persetujuan responden sangat setuju terhadap pertanyaan mengenai kawasan hutan Suaka Margasatwa Gunung Sawal, dan setuju terhadap perntanyaan mengenai keberadaan macan tutul jawa serta konflik.
Keyword: Gunung Sawal, konflik, macan tutul, persepsi

Judul: Penggunaan Koridor sebagai Habitat Macan Tutul Jawa (Panthera pardus melas, Cuvier 1809) di Taman Nasional Gunung Halimun Salak.
Abstrak: Areal koridor Gunung Halimun Salak sangat penting sebagai saluran pertukaran genetik diantara spesies flora dan fauna. Namun sepertiga kawasan hutan koridor telah terganggu akibat aktivitas manusia. Penelitian ini dilakukan untuk mengetahui bagian-bagian kawasan koridor yang sesuai digunakan oleh macan tutul jawa. Data yang diambil meliputi keberadaraan macan tutul jawa, kondisi habitat, dan gangguan habitat. Hasil kamera jebakan ditemukan empat individu yang saling overlap pada wilayah jelajahnya. Ada 18 jenis satwa mangsa dan paling banyak pada hutan sekunder. Ketersediaan air masih tersebar diseluruh kawasan koridor. Nilai kesesuaian habitat diantaranya kelas rendah (11%), sedang (40%), dan tinggi (49%). Bentuk gangguan yang ditemukan adalah penebangan liar, perburuan liar, pembukaan lahan, pencarian rumput serta kegiatan motorcross.
Keyword: macan tutul, kesesuaian habitat, koridor

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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