Judul: Prevalensi anemia dan perilaku makan remaja puteri di SMUN 2 Kuningan kabupaten Kuningan
Abstrak: Penelitian ini secara umum bertujuan untuk mengetahui prevalensi anemia dan perilaku makan remaja puteri SMUN 2 Kuningan. Adapun tujuan khususnya adalah untuk mengetahui prevalensi anemia remaja puteri, karakteristik contoh dan keluarganya, pengetahuan tentang anemia, sikap tentang anemia, kebiasaan makan, dan tingkat konsumsi protein, zat besi, vitamin A dan vitamin C, serta mengetahui perbedaan pengetahuan, sikap, kebiasaan makan, dan tingkat konsumsi antara remaja puteri anemia dengan non anemia. Penelitian ini dilakukan di SMUN 2 Kuningan yang berada di Kabupaten Kuningan, Jawa Barat, dari Bulan April sampai Mei 2001. Contoh dalam penelitian ini adalah remaja puteri Kelas 1 dan Kelas 2. Jumlah contoh diambil secara proporsional adalah 20% dari jumlah siswa yang diperiksa (236) yakni 48 orang yang diambil secara Systematic Sampling. Data yang dikumpulkan adalah data primer dan data sekunder. Data primer meliputi kadar Hb untuk menentukan status anemia dengan menggunakan metode Sahli, keadaan umum contoh, keadaan sosial ekonomi keluarga, pengetahuan, sikap, kebiasaan makan yang dikumpulkan melalui kuesioner, serta konsumsi pangan dengan metode recall. Data sekunder meliputi keadaan umum daerah dan sekolah. Analisis data dilakukan secara statistik deskriptif dan inferensial yang menggunakan uji Man Whitney U dan t-test dengan menggunakan program komputer Excel 2000 dan SPSS 10,0 for windows.
Keyword: anemia

Judul: Pola Konsumsi, Status Gizi, Dan Status Anemia Pada Remaja Putera Dan Puteri
Abstrak: Tujuan umum penelitian adaiah untuk mengetahui pola konsumsi, status gizi dan status anemia pada remaja putera dm puteri di SMUN 1 Kecamatan Guguk Kabupaten Lima Puluh Kota. Adapun tujuan khusus penelitian ini adalah (1) Mengethi ksrakteristik remaja (umur, uang saku, jenis kelamin, aktivitas fisik, keadaan kesehatan, pengetahuan gizi, pendidikan dan peke jaan orangtua, serta besar keluarga). (2) Mengetahui pola konaumsi dan tingkat konsumsi zat gizi (energi. protein, Fe, vitamin C) remaja putera dan puteri. (3) Menganalisis perbedaan status gizi dan status anemia remaja putera dan puteri. (4) Menganalisis pengaruh tingkat pengetahuan gizi, konsumsi zat gizi (energi, protein, vitamin C dan Fe), total aktivitas fisik dan jenis kelamin terhadap status gizi dan status anemia remaja.
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Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable