Judul: Study on the Effect of Vegetation on Thermal Comfort in City Parks in Central Bogor Sub-district
Abstrak: Taman kota merupakan salah satu jenis ruang terbuka hijau publik yang dapat dimanfaatkan sebagai ruang terbuka hijau. Vegetasi pada suatu taman dapat membuat lingkungan terasa nyaman karena kehadirannya dapat memperindah dan mengubah iklim mikro pada kawasan tersebut. Taman Kencana dan Taman Malabar merupakan taman terluas di Kecamatan Bogor Tengah dengan luas masing-masing 4796 m2 dan 5518 m2. Tujuan penelitian ini adalah untuk mengidentifikasi faktor-faktor yang mempengaruhi kenyamanan termal dan rekomendasi konsep vegetasi untuk meningkatkan kenyamanan termal taman kota dan sekitarnya di Kecamatan Bogor Tengah. Penelitian dilakukan dengan metode perencanaan Simonds yang terdiri dari tahapan persiapan, penelitian/pengumpulan data, sintesis, dan perencanaan dengan menggunakan metode analisis data deskriptif kuantitatif. Hasil penelitian menunjukkan bahwa terdapat korelasi yang signifikan antara suhu permukaan dan suhu udara di Taman Kencana dan Taman Malabar dengan nilai koefisien korelasi r = 0,781 dan r = 0,849 secara berurutan. Besaran pengaruh luas vegetasi dan lahan terbangun secara simultan di Taman Kencana terhadap LST (Land Surface Temperature), suhu udara, RH (Relative Humidity), dan THI (Temperature Humidity Index) secara berurutan adalah sebesar 70%; 64,4%; 43,6%; dan 62,9%. Besaran pengaruh luas vegetasi dan lahan terbangun secara simultan di Taman Malabar terhadap LST, suhu udara, RH, dan THI adalah sebesar 65,7%; 67,1%; 13,5%; dan 71%. Luas vegetasi berpengaruh secara signifikan terhadap LST, suhu udara, RH, dan THI di kedua taman. Pada Taman Kencana, 69,7% LST dipengaruhi oleh luas vegetasi, 64% suhu udara dipengaruhi oleh luas vegetasi, 43,3% RH dipengaruhi oleh luas vegetasi, dan 61,8% THI dipengaruhi oleh luas vegetasi. Pada Taman Malabar, 65,2% LST dipengaruhi oleh luas vegetasi, 66,2% suhu udara dipengaruhi oleh luas vegetasi, 9% RH dipengaruhi oleh luas vegetasi, dan 71% THI dipengaruhi oleh luas vegetasi. Luas vegetasi yang harus dioptimalkan dalam satu grid secara efektif untuk mencapai kenyamanan termal ideal di Taman Kencana dan Taman Malabar dengan nilai THI 28,6 secara berurutan yaitu sebanyak 366 m2 dan 349 m2 terutama pada grid area prioritas. Area yang menjadi prioritas untuk diterapkan vegetasi meliputi area parkir, plaza, pathway, pekarangan, dan rooftop., City parks are open public spaces that offer various recreational activities. The presence of greenery in a park can create a comfortable environment and enhance its beauty by altering the microclimate of the area. Kencana Park and Malabar Park are the two biggest parks in Central Bogor Sub-district, with areas covering 4796 m2 and 5518 m2, respectively. The objective of this study is to identify the factors that impact thermal comfort in these parks and suggest vegetation concepts that can enhance the thermal comfort of city parks in Central Bogor Sub-district. The research conducted used the Simonds planning method, which involves preparation stages, data collection/research, synthesis, and planning using quantitative descriptive data analysis techniques. The research findings indicate a significant correlation between surface temperature and air temperature in Kencana Park and Malabar Park with correlation coefficient values of r=0,781 and r=0,849, respectively. In Kencana Park, the vegetation area and built-up land have a 70%, 64,4%, 43,6%, and 62,9% influence on Land Surface Temperature (LST), air temperature, Relative Humidity (RH), and Temperature Humidity Index (THI), respectively. In Malabar Park, the vegetation area and built-up
Keyword: thermal comfort, urban park, vegetation

Judul: Identification of Thermoscape Characteristics of Urban Parks, Bogor City
Abstrak: Keberadaan ruang terbuka hijau perkotaan sangat penting bagi terciptanya ruang publik yang dapat mendukung berbagai aktivitas publik warga kota dan menjadi ruang ekologis bagi terciptanya kualitas lingkungan perkotaan yang memadai. Kota Bogor merupakan salah satu kota yang saat ini sedang mengembangkan ruang terbuka hijau berupa taman kota untuk memenuhi kebutuhan ruang publik sebagai sarana berkumpul dan berekreasi. Kenyamanan termal sangat penting dan harus diperhatikan dalam ruang terbuka hijau. Untuk memperoleh kondisi termal suatu lanskap, penelitian ini menggunakan thermal drone. Penelitian ini bertujuan untuk mengidentifikasi karakteristik thermoscape elemen lanskap dan persepsi pengunjung serta hubungan antara keduanya. Hasil thermoscape pada kedua taman kota berbeda karena adanya perbedaan cuaca. Namun, kondisi termal masing-masing elemen lanskap hampir sama di kedua taman. Hardscape di kedua taman tersebut dapat bersuhu >40˚ C, sedangkan vegetasi, terutama pepohonan, dapat bersuhu <20˚ C. Menurut responden, kedua taman tersebut masih dianggap hangat berdasarkan sensasi termalnya. Thermoscape (nilai RIA) dan persepsi responden mempunyai korelasi kuat dan hubungan searah. Selain itu, hasil analisis regresi kedua taman menunjukkan bahwa nilai RIA berpengaruh positif terhadap persepsi responden. Kawasan taman kota yang panas dapat dimodifikasi dengan menyediakan ruang hijau atau perairan yang lebih luas dengan distribusi dan proporsi elemen yang baik sehingga memberikan kenyamanan bagi penggunanya., The existence of urban green open space is very important for the creation of a public space that can support various public activities of urban residents and become an ecological space for the creation of an adequate quality urban environment. Bogor City is one of the cities that is currently developing green open space in the form of an urban park to meet the needs of public space as a means of gathering and recreation. Thermal comfort is very important and must be considered in a green space. To obtain the thermal conditions of a landscape, this research uses a thermal drone. This study aims to identify the thermoscape characteristics of landscape elements and people's perceptions of it and also the correlations between them. The thermoscape results in the two urban parks are different due to differences in weather. However, the thermal conditions of each landscape elements are almost the same in both parks. Hardscapes in both parks can have a temperature of >40˚ C, while vegetation, especially trees, can have a temperature of <20˚ C. According to respondents, both parks are still considered warm based on their thermal sensation. Thermoscape (RIA value) and respondent perception have a strong correlation and a unidirectional relationship. Besides that, the results of the regression analysis for both parks show that the RIA value has a positive effect on respondents' perceptions. Hot urban park areas can be modified by providing wider green space or water with a good distribution and proportion of elements so as to provide comfort for users.
Keyword: elemen lanskap, kenyamanan termal, sensasi termal, thermal drone, thermoscape

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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