Judul: Ekspresi Gen Ethylene-Responsive Transcription Factor ERF71 pada Sengon (Falcataria moluccana) Sehat dan Terserang Penyakit Karat Tumor
Abstrak: Adanya epidemi penyakit karat tumor pada sengon yang disebabkan oleh cendawan Uromycladium falcatarium dapat mengancam produktivitas kayu sengon dan berdampak pada industri kehutanan di Indonesia khususnya di pulau Jawa. Upaya pengendalian melalui pendekatan secara biologi molekuler dapat digunakan untuk pengembangan sengon yang tahan terhadap serangan penyakit karat tumor melalui proses pemuliaan pohon yang didasarkan pada informasi gen. Tujuan penelitian ini adalah membandingkan hasil kuantifikasi tingkat ekspresi gen Ethylene-responsive transcription factor ERF71 pada kambium pohon sengon yang sehat dan terserang penyakit karat tumor dengan menggunakan teknik Real Time PCR. Data dianalisis berdasarkan metode Relative quantification menggunakan persamaan metode 2-ΔΔCT. Ekspresi gen ERF71 pada sampel sengon yang sehat adalah 2,81; 7,50 dan 1,57. Ekspresi gen ERF71 pada sampel sengon yang terserang penyakit karat tumor adalah 3,89; 1,69 dan 1,90. Analisis ekspresi gen ERF71 pada sengon yang sehat dan terserang penyakit karat tumor memiliki tingkat yang bervariasi., The existence of an epidemic of gall rust disease on sengon caused by the fungus Uromycladium falcatarium can threaten the productivity of sengon wood and have an impact on the forestry industry in Indonesia, especially in Java. Control efforts through a molecular biology approach can be used for the development of sengon that is resistant to gall rust through a tree breeding process based on gene information. The purpose of this study was to compare the results of quantification of the expression level of the Ethylene-responsive transcription factor ERF71 gene in the cambium of healthy and infected sengon trees using Real Time PCR technique. Data were analyzed based on the Relative quantification method using the 2-ΔΔCT method. ERF71 gene expression in healthy sengon samples respectively were 2.81; 7.50 and 1.57. The expression of the ERF71 gene in samples of sengon affected by gall rust respectively were 3.89; 1.69 and 1.90. Analysis of ERF71 gene expression in healthy and infected sengon with gall rust disease has varying levels.
Keyword: Falcataria moluccana, ERF71, qPCR, Relative quantification

Judul: Ekspresi Gen HSP 70 pada Sengon (Falcataria moluccana Miq.) Resisten dan Terserang Hama Boktor
Abstrak: Pohon sengon (Falcataria moluccana Miq.) merupakan salah satu pohon serba guna yang ada di Indonesia. Keberhasilan pembangunan hutan tanaman sengon dapat dipengaruhi oleh beberapa faktor pembatas pertumbuhan, salah satunya adalah ketahanan terhadap serangan hama dan penyakit tanaman. Jenis hama yang paling umum diketahui adalah serangan hama penggerek batang (Xystrocera festiva Thombs.) atau sering dikenal sebagai boktor atau uter-uter. Secara alami tanaman sengon memiliki gen ketahanan terhadap serangan hama boktor, salah satunya yaitu gen Heat shock cognate 70 kDA protein (HSP 70). Penelitian ini bertujuan untuk menguji tingkat ekspresi gen HSP 70 secara kuantitatif terhadap kambium pohon sengon resisten dan sengon terserang hama boktor melalui teknik Real Time PCR. Nilai referensi yang dipilih berupa level ekspresi housekeeping gene menggunakan gen Actin 1 dan gen target menggunakan gen HSP 70, melalui perhitungan ΔCt = Cttarget gene – Cthousekeeping gene. Hasil menunjukkan gen HSP 70 lebih besar terekspresi pada sampel resisten dibandingkan dengan sampel terserang boktor., Sengon (Falcataria moluccana Miq.) is one of the most multipurpose trees in Indonesia. The success of sengon plantation forest development can be influenced by several growth limiting factors, one of these is resistance against pest and disease. The most common type of pest known is stalk borer (Xystrocera festiva Thombs.) or often known as boktor or uter-uter. Naturally, sengon plants have genes for resistance to boktor, one of them is the Heat shock cognate 70kDA Protein (HSP 70) gene. The study aims to test the expression of a gene HSP 70 in a quantitative manner against the cambium trees resistance and sengon attacked by boktor through Real Time PCR technique. The reference level selected housekeeping gene expression in the form of using genes Actin 1 and gene HSP 70 is used, trought calculation ΔCt = Cttarget gene – Cthousekeeping gene. Results showing that genes of HSP 70 greater expression in samples of resistance than infested by boktor.
Keyword: comparative CT method, Falcataria moluccana, HSP 70

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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