Judul: Identifikasi Molekuler Spesies Btm 11, Isolasi, Karakterisasi, Dan Uji Aktivitas Inhibisi Protein Lektin
Abstrak: BTM 11 merupakan kode untuk mikroalga yang diisolasi dari perairan laut Batam, dengan lokasi spesifik yaitu pada titik/stasiun ke-11 di area pengamatan. BTM 11 telah dikembangkan oleh Laboratorium Biorekayasa Lingkungan Pusat Penelitian Bioteknologi LIPI Cibinong Bogor. Mikroalga ini memiliki senyawa bioaktif polisakarida dan flavonoid. Salah satu protein yang terdapat dalam mikroalga adalah protein lektin. Lektin merupakan protein non-imunoglobulin (bukan antibodi) yang mengikat secara spesifik bagian karbohidrat tertentu. Hasil penelitian sebelumnya melaporkan bahwa lektin mampu bersifat antikarsinogenik dan antivirus. Penelitian ini bertujuan mengidentifikasi spesies BTM 11, mengisolasi, mengkarakterisasi, dan menguji aktivitas inhibisi enzim RNA helikase hepatitis C serta antibakteri potein lektin. Kultivasi pada media Sea Water Batam (SWBT) 10 L menghasilkan biomassa kering 7.24 gram selama 7 hari. Biomassa kering digunakan sebagai sampel untuk identifikasi molekuler melalui tahapan isolasi DNA dan amplifikasi DNA menggunakan gen 16S-rRNA. Produk PCR yang diamplifikasi dengan primer 16S-rRNA menunjukkan ukuran 1500 bp. Tahapan selanjutnya adalah purifikasi gel dan proses sekuensing. Hasil analisis data sekuensing menunjukkan BTM 11 memiliki homologi terhadap Geitlerinema sp sebesar 98%. Biomassa kering hasil kultivasi BTM 11 diisolasi protein lektinnya. Isolasi protein lektin menggunakan pengendapan amonium sulfat 75%. Ekstrak kasar hasil pengendapan amonium sulfat dimurnikan menggunakan kolom filtrasi gel Sephadex G-50. Hasil kromatogram terbaik adalah fraksi 6 dan 7. Hasil isolasi protein lektin menunjukkan nilai aktivitas hambat paling tinggi terdapat pada metabolit ekstraksi yaitu 439.6 mm2/mL. Total protein tertinggi juga terdapat pada metabolit ekstraksi yaitu 288 mg. Aktivitas spesifik tertinggi 11.32 AU/mg dengan kelipatan kemurnian 7.42 pada tahap purifikasi Sephadex G-50. Aktivitas spesifik menunjukkan jumlah aktivitas hambat dalam 1 mg. Nilai kelipatan kemurnian meningkat selama proses ekstraksi sampai dengan purifikasi. Karakterisasi protein lektin dengan Sodium Dodesil Sulfat Poliakrilamid Gel (SDS-PAGE) menunjukkan bobot molekul sebesar 17 kDa. Uji hemaglutinasi bertujuan mengetahui aktivitas aglutinasi protein terhadap darah yang menunjukkan adanya protein lektin. Hasil uji hemaglutinasi dengan sampel darah golongan O menunjukkan nilai titer uji 64 (tanpa dilusi). Aktivitas protein lektin stabil pada suhu 50 oC. Perlakuan penambahan MgCl2 dan CaCl2 menunjukkan aktivitas inhibisi protein lektin tetap stabil. Aktivitas inhibisi diuji menggunakan penghambatan enzim RNA helikase hepatitis C dan antibakteri. Hasil uji penghambatan enzim RNA helikase hepatitis C adalah sebesar 57.90% (dilusi 40x) dan 27.55% (dilusi 80x). Uji antibakteri menunjukkan protein lektin mampu menghambat bakteri Staphylococcus aureus ATCC 6538 dan Salmonella typhii ATCC 25241.
Keyword: mikroalga BTM 11, 16S-rRNA, lektin, RNA helikase hepatitis C, antibakteri

Judul: Isolasi dan pemurnian bahan aktif dari mikroalga BTM 11 sebagai inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus is the cause of hepatitis C disease which has high virulence. Recent therapy using combination of ribavirin and alpha interferon has short efficiency < 80%. Thus, the discovery of new drug is needed. Antiviral drugs can be discovered through molecular target therapy by finding the inhibitor of RNA helicase that play role in viral replication. Inhibitor can be derived from chemical compound produced by microalgae. The aim of this research was to isolate the active compound from microalgae BTM 11 which had inhibitory activity against RNA helicase. Inhibitory activity was measured by releasing of phospate inorganic in colorimetric ATPase assay. Crude extract was fractionated using gel filtration chromatography with methanol in chloroform solvent. The result showed that Fraction 3 has the highest inhibitory activity which (81.205%). Phytochemical test of crude extract indicated positive flavonoids and coumarin. Thin layer chromatography (TLC) analysis with eluent chloroform: methanol gave 2 spots with Rf value of 0.8 and 0.37. Moreover, high performance liquid chromatography (HPLC) analysis showed absorption peak with the highest abundance at the retention time of 12.483 minute and 16.617 minute was 266 nm and 230 nm. According to these of phytochemical, TLC, and HPLC, inhibitor compound from BTM 11 belongs to flavonoids.
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Judul: Dampak Perceraian dan Hubungan Sosial terhadap Kesejahteraan Psikologis Ibu sebagai Orang Tua Tunggal
Abstrak: Family with a single mothers who came from divorced, often bring the impact such as problem or change on a single mothers well-being. The aim of this research to indentify characteristics of single mother and relations with psychological well-being and social relations, and also analyze impact divorce on psychological well-being and social relations. Descriptive analysis, regression, and Pearson correlation was used in this study. The respondents were sixty single mother who come from the length divorced more than six month. The result showed that compare with present condition, psychological well-being, impact of divorce and social relation respondent are more significant lower than the first six month after divorce. There was relationship between age of present and divorce, family’s income, and size of family with level of depression, social support, and economic impact. Based on regression analysis we found there was negative effect of impact of divorce to psychological well-being at the first six month after divorce and the present. Other wise, we found there was not significant effect of divorce to social relationship.
Keyword: economic impact, level of depression, social support