Judul: Genetic Variation of Planted andNatural Agarwood Assessed by Microsatellite.
Abstrak: damar wangidengan keharuman yang khas,sehinggabanyakdigunakan sebagai bahan baku industri parfum, obat-obatan, kosmetik, dupa, pengawet serta untuk keperluan ritual keagamaan. Eksploitasi gaharu yang berlebihanmengakibatkan kelangkaan dan hilangnya pohon (genotipe) unggul. Kelangkaan ini menyebabkan gaharu masuk kedalam daftar CITES Apendiks II, dengankonsekuensinyayaitupenjualan ekspor dan impor gaharu diaturolehkuota.Akan tetapi, kuotainitidak membedakan gaharu alami atau budidayasehingga masihmendorong terjadinyapraktek penebangan liar.Olehkarenaituperludilakukanupayapengendalian yang efektfterhadapasalusulkayugaharu. Akhir-akhirinipenggunaanpenanda DNA menjadipentinguntukmemverifikasiasal-usulkayudanbahantanamankarenasifatnya yang sulitdimanipulasisehinggaperludiujicobapadakayugaharu.Penelitianinibertujuanuntuk 1) melakukanamplifikasisilang primer mikrosatelitAquilariacrassnakejenisgaharulainnya, dan 2) mendugaasalusulkayugaharuyang diperdagangkan di pasaran.Bahanpenelitianyaitupopulasigaharudarihutanalamdantanamansertakayugaharu yang tidakdiketahuiasal-usulnya.Primer mikrosatelitA.crassna yang digunakandalamamplifikasi DNA gaharuadaempatmacam, yaitu 6pa18 (180-210 bp), 10pa17 (152-156 bp), 16pa17 (143-155 bp), dan 71pa17 (152-224 bp).Berdasarkanhasilpengamatandidapatkanbahwasemuajenissampelgaharubaikdarihutan alammaupunhutantanamandapatmengamplifikasisilangpadarentangukuranfragmen DNA yang diharapkan.Analisisgenetikmenunjukkannilaikeragamangenetik (He) dalampopulasigaharusebesar He=0.5443 (hutantanaman), dan He=0.4642 (hutanalam). Nilaikeragamangenetiktersebutdapatdigunakansebagaiinformasiilmiahuntukupayapengko nservasiangaharu.PadadendrogramNtsys (analisisklaster) terlihatadanyapengelompokangaharuasalhutantanamandanhutanalam, dimanasampelpotongan kayu gaharu dengan asal-usul tidak jelas mengelompokke gaharudari hutanalam. Hal inimengindikasikanbahwakayu gaharu yang diperdagangkan masih berasal dari hutan alam.
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Judul: Keragaman Genetik Tanaman Penghasil Gaharu Aquilaria malaccensis berdasarkan Penanda Mikrosatelit
Abstrak: Aquilaria malaccensis is a high quality agarwood producing tree of which population in nature has become rare and almost extinct due to over-exploitation. This research aim to estimate the genetic variation of innoculated A.malaccensis seedling and tree populations using microsatellite marker and to determine the correlation between the plant genotypes and the agarwood components based on analysis using GCMS. DNA was extracted using Dneasy plant mini kit from QIAGEN, and then amplified using 4 microsatellite primer pairs, i.e. 6pa18, 10pa17, 14pa17, and 16pa17. However, only two primer pairs, i.e. 6pa18 and 16pa17 were able to cross-amplify and produced polymorphic fragments with different sizes. In microsatellite locus of 16pa17 the presence of one specific allele was detected which could differentiate the plant that potentially able to produce the large amount of agarwood. The estimated value of genetic variation of the populations studied was categorized as high (He = 0.31). This variation was spread as much as 63% within populations and 37% among populations.
Keyword: microsatellite, genetic variation, genetic structure, A.malaccensis

Judul: Penyelesaian Masalah Rotasi Aliran Fluida Kental Von Karman Menggunakan Metode Homotopi
Abstrak: Von Karman equation is an equation that describes fluid viscous flow induced by infinite disk rotation. By assuming steady flow and laminar, viscous incompressible fluid flow is represented in Von Karman equation by an angular velocity and the vertical direction velocity as the independent variables. Von Karman equation is a nonlinear problem that is solved using the homotopy method. The use of homotopy method is done by defining an homotopy function that requires auxiliary parameters to control the convergent region of the solution. The solution is a recursive formula with given initial conditions. Using software based-functional shows that velocity components converge to a value.
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