Judul: Karakterisasi Gen Ghrelin (GHRL) pada Ayam Kampung Ayam Leher Gundul Ayam Walik dan Ayam Arab
Abstrak: Ghrelin gene (GHRL) is one of important gene that stimulates secretion of gastric acid, growth hormone and food intake. Sekuens of GHRL fragment in this study was located in promoter, exon 1 and partial intron 1. This study aimed to characterize GHRL gene especially to detect mutation sites of kampong, naked neck, walik, and arab chicken. Twenty samples were amplified to result of fragment with length of 1 050 bp. Characterization of GHRL gene was processed by BLAST-N, then MEGA 5 was used to analize the sequence data. Annealing temperature was performed at 60 ºC for 1 minute. The alignment product of eight samples by using primer forward produced sequence of 554 bp. The similarity value of GHRL gene fragment with sequence data on GenBank (AB158617) was 99%. There was found 3 mutation sites of GHRL gene fragment. However, the 554 bp of GHRL gene fragment has low power to distinguish chicken breeds since all the samples have pairwise distance based on the phylogenetic tree construction analysis.
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Judul: Karakterisasi Gen Prolaktin (PRL) pada Ayam Kampung Ayam Walik Ayam Leher Gundul dan Ayam Arab
Abstrak: Indigenous and local chicken germ plasm in Indonesia have a high potency to be developed. One of the potency that can be developed is an improvement egg production. Low egg production of those chickens can be increased by selecting the in broodiness. Broodiness is controlled by prolactin gene (PRL). Since the characteristics of PRL gene of kampung, walik, leher gundul and arab are still limited refence, therefore this study aim to investigate the characteristic of PRL gene on their. Blood samples were extracted to obtain DNA. PCR then was used to amplified the fragment of PRL. A total of 20 samples successfully amplified at annealing temperature of 60 °C for 45 seconds and then directly sequenced. DNA sequencing results were analyzed using the MEGA 5 program. PRL gene in this study had similarity of 98%-99% with the PRL gene sequence data available on GenBank. This study also shows that there were 11 point mutation of PRL gene’s fragment with the type of mutations were deletions, insertions, transitions and transvertion. Those mutation types have potency as new single nucleotide polymorphisms (SNPs).
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Judul: Sintesis Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) dengan Variasi Konsentrasi Sulfur pada Substrat Kaca ITO
Abstrak: Film tipis Cu2ZnSnS4 (CZTS) merupakan bahan alternatif semikonduktor sebagai lapisan penyerap cahaya pada sel surya. CZTS dapat menggantikan senyawa silikon yang biasa digunakan sebagai bahan penyusun semikonduktor komersial. Kelebihan CZTS sebagai lapisan penyerap cahaya ialah ketersediaan bahan yang berlimpah di alam dan nilai koefisien absorpsi yang tinggi. Film tipis CZTS dibuat dengan variasi konsentrasi larutan sulfur menggunakan metode sol- gel dan spin coating sederhana pada substrat kaca inidium timah oksida (ITO). Setelah itu, film dikeraskan pada suhu 550 °C, selama 60 menit dengan dialiri gas N2. Difraktogram sinar X menunjukkan bahwa film tipis CZTS yang terbentuk dari kedua sampel memiliki struktur kristal kasterit dengan persentase yang kecil dibandingkan literatur. Sampel dengan konsentrasi sulfur 6M memiliki jumlah persen kristal yang lebih tinggi, yaitu 13,257% dengan ketebalan film 3,726–4,732 µm, sedangkan sampel dengan konsentrasi 3M memiliki persentase 7,538%. Sampel film tipis CZTS dengan konsentrasi sulfur 6M memiliki energi celah pita sebesar 1,876 eV dan nilai koefisien absorpsi sebesar 29,49 × 104 cm-1 menunjukkan hasil terbaik dan sesuai dengan kriteria film tipis CZTS komersil.
Keyword: CZTS thin films, ITO glass, Semiconductors, Sol-gel, Spin coating