Judul: Analisis Titik Panas (Hotspot) dan Curah Hujan di Kabupaten Mappi, Provinsi Papua Selatan
Abstrak: Kabupaten Mappi merupakan salah satu wilayah di Provinsi Papua Selatan yang memiliki jumlah hotspot tertinggi setelah Merauke dalam satu dekade terakhir. Penelitian bertujuan menganalisis pola sebaran hotspot dan keterkaitannya dengan curah hujan pada periode 2012-2021 di Kabupaten Mappi, Provinsi Papua Selatan. Sumber yang digunakan dalam penelitian adalah data hotspot dari citra satelit MODIS Terra/Aqua dan data curah hujan dari NASA LaRC Power yang dianalisis menggunakan analisis deskriptif dan analisis regresi. Hasil penelitian menunjukkan adanya fluktuatif jumlah hotspot pada rentang tahun 2012-2021 dan puncak hotspot terjadi pada tahun 2015. Jumlah hotspot pada rentang tahun tersebut adalah 3879 titik. Sebanyak 1415 titik (36,48%) tersebar pada lahan gambut dan 2464 titik (63,52%) pada lahan mineral. Hasil analisis regresi menunjukkan bahwa hubungan antara hotspot dengan curah hujan memiliki nilai p-value 0,006, artinya p-value < 0,05 menunjukkan korelasi keduanya berpengaruh secara signifikan.
Keyword: forest and land firest, hotspot, Mappi District, rainfall

Judul: Hotspot and Rainfall Analysis Period in Mappi District, South Papua Province
Abstrak: Kabupaten Mappi merupakan salah satu wilayah di Provinsi Papua Selatan yang memiliki jumlah hotspot tertinggi setelah Merauke dalam satu dekade terakhir. Penelitian bertujuan menganalisis pola sebaran hotspot dan keterkaitannya dengan curah hujan pada periode 2012-2021 di Kabupaten Mappi, Provinsi Papua Selatan. Sumber yang digunakan dalam penelitian adalah data hotspot dari citra satelit MODIS Terra/Aqua dan data curah hujan dari NASA LaRC Power yang dianalisis menggunakan analisis deskriptif dan analisis regresi. Hasil penelitian menunjukkan adanya fluktuatif jumlah hotspot pada rentang tahun 2012-2021 dan puncak hotspot terjadi pada tahun 2015. Jumlah hotspot pada rentang tahun tersebut adalah 3879 titik. Sebanyak 1415 titik (36,48%) tersebar pada lahan gambut dan 2464 titik (63,52%) pada lahan mineral. Hasil analisis regresi menunjukkan bahwa hubungan antara hotspot dengan curah hujan memiliki nilai p-value 0,006, artinya p-value < 0,05 menunjukkan korelasi keduanya berpengaruh secara signifikan., Mappi Regency is one of the areas in South Papua Province which has the highest number of hotspots after Merauke in the last decade. The research aims to analyze the pattern of hotspot distribution and its relationship to rainfall in the 2012-2021 period in Mappi District, South Papua Province. The sources used in this study were hotspot data from the MODIS Terra/Aqua satellite imagery and rainfall data from NASA LaRC Power which were analyzed using descriptive analysis and regression analysis. The results showed that there was a fluctuation in the number of hotspots in the 2012-2021 range and the peak of hotspots occurred in 2015. The number of hotspots in that year range was 3879 points. A total of 1415 points (36,48%) are spread on peat land and 2464 points (63,52%) on mineral land. The results of the regression analysis show that the relationship between hotspots and rainfall has a p-value of 0,006, meaning that the p-value is < 0,05 which indicates that the correlation between the two has a significant effect.
Keyword: forest and land fires, hotspot, Mappi District, rainfall

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.