Judul: Desain Model Kurikulum Berbasis Go Green School
Abstrak: Population is increasing, on the other hand the availability of natural resources remains the same, hence, it is necessary to use advanced technology to increase productivity and to use natural resources properly for the prosperity of human beings. But this could also give negative impacts to the environment. Egoism and moral crisis are considered as factors which cause the environment destruction. This situation needs to be overcome through strategic way to young generation on character building which is integrated in the curriculum. The Go Green School Model (GGS) curriculum design of Tarakanita is based on the Education Unit Level Curriculum (EULC). System approach is used for designing the ideal mode is based on the 8 criteria of National Education Standards as stipulated in the Government Bylaws No. 19 in 2005. The performance of the model is then compared to currently-used curriculum and validated to the pre-determined sample of fourth-grade elementary students for one semester. The result shows that the understanding and creativity ( factors of environmental content) of the students treated using the curriculum of GGS tend to be higher than that of the students treated using the Education Unit Level Curriculum . To shift from the Education Unit Level Curriculum Curriculum to the GGS curriculum needs a linking step that is the Teaching Unit Curriculum Plus (EULC +).
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Judul: Komunikasi Partisipatif Pada Pelaksanaan Pendidikan Lingkungan Hidup Green School Di Kecamatan Cicurug Kabupaten Sukabumi
Abstrak: Damage Mist Mountain Salak National Park caused by the activities of people who still throw garbage into rivers, illegal logging (illegal logging) in the forest area of Mount Salak area and river basin management that reduce or eliminate the water- absorbing buffer trees to the river and the sand mining activities that have the potential causing landslides. Damage triggered by the presence of land in agricultural activities on marginal lands that should be a water catchment area. Rural communities in Pasawahan, village and village Kutajaya Cisaat directly adjacent to the Mist Mountain Salak National Park (TNGHS) had problems related to the economy, natural resources, and human resources. This problem is in the trigger by limited capacity and skills within communities to manage natural resources. Rural communities in Pasawahan, village and village Cisaat Kutajaya want to create harmony and balance in terms of ecological and environmental economics. People hope there are efforts to preserve the environment and is able to provide enhanced level of real economic Environmental Education Program (PLH) Green School is a proposed education program, conducted and designed for the needs of the community. PLH Green School program is one program that is generated by the proposed agreement by representatives Pasawahan villagers, village Cisaat and village Kutajaya concerned and aware of the efforts to resolve the problems perceived by the public about environmental values and issues of environmental issues. PLH Green School program was accompanied by a facilitator who aims to improve the knowledge, attitudes and skills of the community with community participation. Participatory communication is expected to remove obstacles, especially in terms of information exchange as well as inequalities in the implementation issues underlying this research are: 1) how participatory communication that occurs in PLH Green School Program ? 2) how the relationship between individual characteristics, the credibility of the communicator and institutional support participatory communication PLH Green School Program ? 3) how participatory communication links with behavior change PLH Green School Program ? 4) how does the behavior change with practice participants PLH Green School Program ? The purpose of research is to 1) describe participatory communication in PLH Green School Program, 2) analyze the relationship between individual characteristics, the credibility communicator and institutional support to participatory communication PLH Green School Program; 3) analyze the relationship between participatory communication to behavior change PLH Green School Program; 4) analyze the relationship between changes in behavior and practice PLH Green School Program participants. Analysis of the correlation between the variables of individual characteristics with participatory communication shows that age, education, motivation and ownership of the media is not significantly correlated to the direction of participatory communication is communication, communication and participation channels. Expertise and attractiveness facilitators have highly significant relationship at p<0.01 by way of communication, but not significantly correlated in the channels of communication and participation. Familiarity variables significantly correlated at p<0.05 in the channels of communication and participation. Honesty variables not significantly correlated with the direction of the communication, the channel and the level of participation. Correlation between the direction of the communication facilitator expertise in implementing PLH Green
Keyword: partipatory communication, environmental education program, green School.

Judul: Stabilitas Protein Rekombinan Interferon Alfa-2b Manusia Pada Pichia Pastoris.
Abstrak: Protein rekombinan interferon alfa-2b manusia (rhIFNα-2b) merupakan protein terapeutik yang diaplikasikan untuk penanganan hepatitis dan kanker. Pengembangan bentuk sediaan rhIFNα-2b yang stabil tetap menjadi tantangan. Penelitian ini bertujuan untuk mempelajari stabilitas rhIFNα-2b pada tiga parameter, yaitu suhu, pH dan kondisi lama penyimpanan. Overproduksi rhIFNα-2b dilakukan menggunakan sistem ekspresi Pichia pastoris (P. pastoris) yang ditumbuhkan pada media Buffered Methanol Complex (BMMY) pada suhu 30 °C selama 48 jam dan 2% metanol sebagai induser. MinimateTM tangensial flow filtration system dengan molecular weight cut off (MWCO) 5 kDa digunakan pada proses filtrasi protein. Protein yang telah difiltrasi kemudian dipurifikasi dengan AKTA purifier 10 system menggunakan kolom His-trap. Kuantifikasi protein menggunakan bicinchoninic acid assay menunjukkan bahwa yield rhIFNα-2b hasil purifikasi sebesar 10.92 mg/L (OD600 = 2.3). Karakterisasi bobot molekul menggunakan analisis Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) dan Western Blot menunjukkan bahwa protein tersebut merupakan rhIFNα-2b dengan bobot molekul sekitar 24 kDa. Karakterisasi identitas protein berbasis spektrometri massa Matrix Assisted Laser Desorption Ionization-Time Of Flight/ Time Of Flight (MALDI-TOF/ TOF) menginformasikan bahwa protein tersebut teridentifikasi sebagai hIFNα-2b dengan urutan asam amino yang mewakili 22% dari sekuen secara keseluruhan. Analisis SDS-PAGE non reducing dan software Image J menunjukkan bahwa peningkatan suhu, kondisi pH asam dan basa serta kondisi lama penyimpanan telah memacu terjadinya aggregasi dan degradasi rhIFNα-2b. Uji 3-[4.5-dimethyltiazol-2il]-2.5-diphenylltetrazolium bromide (MTT) menunjukkan bahwa aggregasi dan degradasi menurunkan aktivitas antiproliferasi rhIFNα-2b terhadap sel MCF-7. Kondisi kenaikan suhu, pH asam dan basa serta lama penyimpanan dapat mempengaruhi stabilitas rhIFNα-2b. Kondisi stabil rhIFNα-2b dicapai pada suhu 4 °C, pH 7 selama 2 hari waktu inkubasi. Data tersebut dapat digunakan untuk mengembangkan formulasi rhIFNα-2b sebagai protein terapeutik.
Keyword: rhIFNα-2b, Pichia pastoris, stabilitas, aggregasi, degradasi