Judul: Pemurnian Parsial Dan Karakterisasi Glukosa Oksidase dari Isolat Aspergillus niger (IPBCC.08.610)
Abstrak: The increasing application of enzymes in industry, healthcare and energy initiates production rise of enzymes from microorganisms, one of them are glucose oxidase from Aspergillus niger, which is now develop as enzymatic fuel cell. The aim of this study was to purified and characterized the enzyme glucose oxidase from Aspergillus niger (IPBCC.08.610). Partial purification using amonium sulfate precipitation and then followed by dialysis. The purification fold of dialysis enzyme was 6.17, specific activity enzyme was 43.17 Umg-1. In addition, the activity of glucose oxidase was stable at 25 oC. However, the result of ion stabilization of the enzyme showed that the activity of glucose oxidase reduced by the presence of Ag+ ion with relative activity was 4.8% in 1 mM and 2.1% in 5 mM. Thiourea inhibit the relative activity to 58.44% in 1 mM. On the other hand, the molecular weight of enzyme unit was estimated 160 kDa and there are 2 protein subunits of the glucose oxidase with approximately molecular weight 80 kDa. At the end, in the aspect of enzyme kinetics, glucose oxidase had Km and Vmaks value (glucose as a substrate) respectively 43 mM and 33.67 Umg-1
Keyword: partial purification, glucose oxidase, characterization, Aspergilus niger (IPBCC.08.610)

Judul: Pemurnian dan Karakterisasi Glukosa Oksidase dari Isolat Aspergillus niger (IPBCC 08.610)
Abstrak: Glukosa oksidase (GOD) sedang banyak dikembangkan dalam berbagai aplikasi seperti pada industri makanan, obat-obatan, dan elektronik. Sumber GOD terbaik berasal dari Aspergillus niger karena diketahui memiliki aktivitas spesifik yang tinggi. Penelitian ini bertujuan untuk memurnikan glukosa oksidase dari A.niger (IPBCC 08.610) dengan menggunakan teknik kromatografi kolom gel filtrasi dan karakterisasinya. Pemurnian menggunakan kromatografi filtrasi gel menghasilkan satu fraksi enzim yang memiliki kadar protein lebih tinggi (Fraksi 4) dibandingkan fraksi lainnya. Aktivitas spesifik fraksi hasil kromatografi adalah 31.92 U/mg dengan tingkat kemurnian 8.86 kali dibandingkan dengan ekstrak enzim kasar yaitu 3.61 U/mg. Estimasi bobot molekul hasil analisis metode SDS-PAGE menunjukkan 2 pita protein yang berukuran 161 kDa dan 84 kDa. Adapun nilai Km dan Vmaks glukosa oksidase dengan substrat glukosa adalah 39 mM dan 3.7 x 10-3 mmol/det.
Keyword: Aspergillus niger, glukosa oksidase, Km, Vmaks, kromatografi kolom filtrasi gel, pemurnian, SDS-PAGE

Judul: Implementasi Space Time Composite (STC) Data Model Menggunakan Model Data Relasional
Abstrak: The data model is a concept that describes the data stored in the database and the relationship between the data to the user in logic. Development of data models is very important in the design of the database as the basis of the stage of database design to be made. This study aims to apply the concept of Space Time Composite (STC) on the Model Data storage vector data types that can store enough information and describes the process of drafting objects and objects spatiotemporal evolution over time. From the results of the test queries on the data model that have been created, queries in a system are acquired that can help display the information preparation of objects from time to time and know the history of the object polygon and arc that has undergone changes in topology.
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