Judul: Studi Komparasi Konsep Diri, Kelompok Acuan, Strategi Pemasaran, dan Perilaku Pembelian Online Antargenerasi
Abstrak: Perilaku pembelian online adalah suatu tingkah laku nyata yang dilakukan konsumen dalam membeli suatu barang melalui internet. Penelitian ini bertujuan untuk membandingkan dan menganalisis pengaruh konsep diri, kelompok acuan, dan strategi pemasaran terhadap perilaku pembelian online pada generasi Baby Boomer, generasi X, dan generasi Y. Penelitian dilakukan dengan wawancara secara langsung di Perumahan Dramaga Cantik dan Kelurahan Pagelaran Ciomas, Kabupaten Bogor. Responden dalam penelitian ini adalah 90 ibu rumah tangga dengan masing-masing 30 responden setiap generasi. Teknik penentuan responden menggunakan quota sampling dengan syarat pernah melakukan pembelian online minimal 1 kali dalam 6 bulan terakhir. Data penelitian menggunakan analisis deskriptif, uji beda menggunakan aplikasi SPSS 22,0, dan uji pengaruh dengan metode SEM melalui program Smart Partial Least Square (PLS). Penelitian menunjukkan pada variabel konsep diri, strategi pemasaran, dan perilaku pembelian online memiliki kategori sedang, sedangkan pada variabel kelompok acuan memiliki kategori rendah. Terdapat perbedaan signifikan pada dimensi actual self concept, promotion dan variabel strategi pemasaran antar ketiga generasi. Konsep diri berpengaruh signifikan terhadap perilaku pembelian online pada semua generasi. Kelompok acuan berpengaruh signifikan terhadap generasi Baby Boomer dan strategi pemasaran berpengaruh signifikan pada generasi Baby Boomer, dan semua generasi.
Keyword: generasi Baby Boomer, generasi X, generasi Y, perilaku pembelian online

Judul: Studi Komparasi Konsep Diri, Gaya Hidup, Strategi Pemasaran, dan Pembelian Impulsif Antargenerasi
Abstrak: Pembelian impulsif adalah tindakan membeli yang sebelumnya tidak diakui secara sadar. Penelitian ini bertujuan untuk membandingkan dan menganalisis pengaruh konsep diri, gaya hidup, dan strategi pemasaran terhadap perilaku pembelian impulsif pada generasi Baby Boomer, generasi X, dan generasi Y. Penelitian ini menggunakan desain cross sectional study dengan wawancara langsung menggunakan kuesioner di DKI Jakarta. Responden sebanyak 90 orang ibu rumah tangga yang dipilih menggunakan metode quota sampling. Analisis data menggunakan one way anova dan SEM-PLS. Hasil penelitian menunjukkan tidak terdapat perbedaan yang signifikan pada variabel konsep diri, gaya hidup, strategi pemasaran, dan pembelian impulsif antara generasi Baby Booomer, generasi X, dan generasi Y. Variabel gaya hidup berpengaruh signifikan terhadap pembelian impulsif pada semua generasi, generasi Baby Boomer, dan generasi X. Variabel lainnya dalam penelitian ini tidak berpengaruh signifikan terhadap pembelian impuslif.
Keyword: faktor analisis, generasi Baby Boomer, generasi X, generasi Y, SEMPLS

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable