Judul: Pengaruh Perbedaan Ukuran Mata Jaring Rampus terhadap Hasil Tangkapan Ikan Layang (Decapterus kurroides) di Perairan Cisolok, Palabuhanratu, Kabupaten Sukabumi
Abstrak: The purpose of this research is to determine the catch composition and diversity of monofilament bottom gillnet of different mesh size, to determine the catch of redtail scad (Decapterus kurroides) by using monofilament bottom gillnet with different mesh size, and to determine the difference of capture mechanism of redtail scad (Decapterus kurroides) on the monofilament bottom gillnet with different mesh size. The research was done by experimental fishing method using monofilament bottom gillnet with different mesh size (44.5; 50.8; 63.5; and 76.2 mm (1.75; 2; 2.5 and 3 inches)). The arrangement of mesh panel on the set of monofilament bottom gillnet was alternated during fishing trials for giving equal opportunities of fish to be caught. The catch diversity is analyzed using the Shannon Wiener index. The statistical test, i.e.,Friedman test and multiple comparison test are used for significantly test of the catch. The result showed that total catch of monofilament bottom gillnet 529 individuals, consisted of six species. The catch dominant was redtail scad (Decapterus kurroides) with number of 351 individuals or 66.35% of the total catch, followed by goldband goatfish (Upeneus moluccensis) with number of 67 individuals or 12.67% of the total catch. Based on the different mesh size of monofilament bottom gillnet, redtail scad was the main catch of monofilament bottom gillnet with number of mesh size 2.5 was 161 individuals (45.87%). Generally, the redtail scad caught in entangled reached in total 269 individuals (76.64%) of which mesh size 1.75 inches caught 28 individuals (10.41%), mesh size 2 inches caught 70 individuals (26.02%), mesh size 2.5 inches caught 129 individuals (47.96%), and mesh size 3 inches caught 42 individuals (15.61%).
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Judul: Pengaruh Perbedaan Mesh Size Jaring Rampus terhadap Hasil Tangkapan Rajungan (Portunus pelagicus) di Teluk Jakarta, Muara Angke
Abstrak: The objectives of this research were to determine the catch composition and diversity of monofilament bottom gillnet in different mesh size, to count the swimming crabs (Portunus pelagicus) caught by monofilament bottom gillnet in different mesh size. This research was conducted by experimental fishing method using monofilament bottom gillnets which have different mesh size (3; 3,5 and 4 inches). The arrangement of mesh panel on the set of monofilament bottom gillnet was operated in turns during fishing trials for giving equal opportunities of fish to be caught. The statistical tests, ANOVA test and BNT test, were used to examine the number of the catch. The result showed that total catch of monofilament bottom gillnet was 405 individuals, consisted of six species. The largest catch was swimming crab (Portunus pelagicus) which made up 235 individuals or 58% of the total catch. Based on the mesh size of monofilament bottom gillnet, it can be seen that swimming crab was the main catch of the 3 inch monofilament bottom gillnet which constituted 126 individuals (54%).
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Judul: Konstruksi Gen Glukosa Oksidase untuk Subkloning ke dalam Plasmid pPICZα dengan Penambahan Oligonukleotida Penyandi Spacer Peptida
Abstrak: Glukosa oksidase (EC 1.1.3.4) merupakan flavoenzim yang berasal dari Aspergillus niger. Enzim Glukosa Oksidase (GOX) intraseluler yang berasal dari A. niger memiliki aktivitas yang lebih tinggi dibandingkan dengan aktivitas enzim GOX ekstraselulernya. Enzim GOX intraseluler yang berasal dari A. niger memiliki tingkat produksi yang rendah serta proses pemurnian yang cukup rumit. Penelitian ini bertujuan untuk mendapatkan gen penyandi GOX yang siap untuk disisipkan ke dalam plasmid pPICZα untuk kemudian diekspresikan pada Pichia pastoris secara ekstraseluler. Perbanyakan plasmid rekombinan pGEM®-T Easy-GOX dilakukan pada sel bakteri Escherichia coli DH5α. Konstruksi gen GOX dilakukan dengan amplifikasi menggunakan PCR. Amplifikasi dilakukan sebanyak dua kali. Ukuran panjang basa yang diperoleh dari hasil amplifikasi kedua sebesar 1787 bp, terdiri atas situs restriksi XhoI dan oligonukleotida penyandi spacer peptida pada ujung 5’ sekuens gen GOX, serta situs restriksi XbaI pada ujung 3’ sekuens gen GOX.
Keyword: glukosa oksidase, pGEM®-T Easy-GOX, PCR, pPICZα