Judul: Viabilitas Polen dan Kultur  Anter Pada Beberapa Kultivar Pisang (Musa spp.)
Abstrak: Kasus alergi banyak dilaporkan terjadi di kecamatan Pasar Minggu Jakarta Selatan. Polen merupakan salah satu jenis alergen pada sistem saluran pernapasan yang belum pernah diteliti di Indonesia. Penelitian ini bertujuan untuk mengetahui alergenisitas polen di udara bebas Pasar Minggu Jakarta Selatan. Polen yang ditemukan beterbangan bebas di daerah tersebut diidentifikasi dan dikarakterisasi berat molekul proteinnya. Pengujian terhadap alergenisitas polen dilakukan secara in vivo dengan menggunakan tikus wistar. Empat jenis polen ditemukan beterbangan di udara bebas kecamatan Pasar Minggu Jakarta Selatan, yaitu: polen jagung, kelapa genjah, pinus, dan kelapa sawit. Konsentrasi protein masingmasing polen yang diuji secara berurutan adalah 65, 88, 14, and 66 mg/gram polen. Hasil SDS-PAGE menunjukkan bahwa polen jagung, kelapa genjah, pinus, dan kelapa sawit berturut-turut mempunyai protein dengan berat molekul antara 17 – 70 kDa; 17 – 68 kDa; 16 - 68 kDa; dan 15 – 68 kDa. Reaksi inflamasi yang ditandai dengan pembengkakan pada kulit terjadi setelah dilakukan penyuntikan ekstrak polen pada bagian bawah kulit (subcutaneous) kaki tikus wistar. Pemaparan ekstrak polen pada saluran pernapasan tikus menyebabkan peningkatan relatif berat saluran pernapasan tikus, penurunan berat badan total tikus, pembesaran sel-sel membran mukosa, sekresi mukus yang berlebihan, dan erupsi sel-sel inflamasi. Semua polen yang diteliti bersifat alergenik pada tikus wistar, sehingga berpotensi digunakan sebagai ekstrak alergen untuk mendeteksi alergi pernapasan serta sebagai bahan imunoterapi penderita alergi di Indonesia.
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Judul: Alergenisitas polen di udara bebas Pasar Minggu Jakarta Selatan pada tikus wistar (Rattus norvegicus).
Abstrak: Allergy cases were frequently reported at kecamatan Pasar Minggu South Jakarta. Pollen is one of the respiratory-system allergen substance which has never been explored in Indonesia. This research was conducted to know the allergenicity of airborne pollen at Pasar Minggu South Jakarta. The protein of airborne pollen at that area was identified and characterized. The detection of allergenicity was made to rat (Wistar strain) by in vivo experiment. There were four kind of airborne pollen found in Pasar Minggu, South Jakarta, which were: maize, dwarf coconut, pine, and oil palm pollen. Their protein concentration were, respectively, 65; 88; 14; and 66 mg/g of pollen. SDS-PAGE results showed that maize, dwarf coconut, pine, and oil palm pollen had several proteins with molecular weight ranging from 17 – 70 kDa; 17 – 68 kDa; 16 - 68 kDa; and 15 – 68 kDa, respectively. Skin inflamation was detected after injection of those protein extract to mouse’s subcutaneous. Respiratory challenge caused an increase in relative weight of respiratory tract. This challenge though, cause a decrease in body weight and an increase in size for mucose membrane cells, a secretion of mucose, and an eruption of inflammation cells. All pollen were allergenic to wistar rat, thus can be utilized as an extract allergen for allergy detection and immunotherapy substances for human in Indonesia.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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