Judul: Pola mendengar siaran radio kisi 93.4 FM dan pengaruhnya terhadap perilaku remaja : kasus pengaruh program siaran kisi-kisi kita terhadap siswa SMUN 7
Abstrak: Dengan berbekal berbagai macam keistimewaannya, radio sebagai media audio memiliki jangkauan khalayak yang luas terutama dari kalangan remaja. Remaja dalam melaksanakan interaksi sosial sehari-hari dengan sesamanya memerlukan pertukaran informasi yang salah satunya didapat dari radio sebagai sumber informasi. Hal ini akan menyebabkan siaran radio dapat memberikan pengaruh terhadap aspek-aspek pengetahuan (cognitive), sikap (affective) dan tindakan (conative) pada remaja. Berdasarkan hal tersebut di atas, tujuan utama penelitian ini adalah untuk mengetahui pola mendengar siaran radio KISI 93.4 FM dan pengaruhnya terhadap perilaku remaja. Tujuan penelitian ini secara rinci adalah sebagai berikut: (1) mengetahui pola mendengar siaran radio pada remaja; (2) mengetahui faktor- faktor yang mempengaruhi perilaku remaja dalam mendengar siaran radio dan (3) mengetahui pengaruh mendengar siaran radio, khususnya program siaran Kisi- Kisi Kita di Radio KISI 93.4 FM terhadap aspek-aspek pengetahuan (cognitive) dan sikap (affective) pada remaja. Penelitian ini dilakukan pada pelajar Sekolah Menengah Umum Negeri (SMUN) 7 Bogor yang merupakan pendengar radio KISI di Kelurahan Bantarjati, Kecamatan Bogor Utara, Kota Bogor, Propinsi Jawa Barat. Kelas yang dipilih untuk kerangka sampling adalah kelas II SMUN 7, yang berusia 16 sampai 18 tahun. Kelas ini dipilih dengan pertimbangan bahwa siswa di kelas tersebut tidak sedang menghadapi ujian. Kemudian dari seluruh kelas II dipilih empat dari sembilan kelas secara purposive. Pemilihan kelas dibantu oleh Kepala Bidang Kurikulum SMUN 7 Bogor. Dari pemilihan tersebut diperoleh kelas terpilih, yaitu kelas II-1, II-5, II-7 dan II-9...dst
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Judul: Perilaku Remaja dalam Mendengarkan Radio Komunitas (Kasus Pendengar BeTe Radio di Kelurahan Tegal Gundil, Kecamatan Bogor Utara, Kota Bogor, Provinsi Jawa Barat)
Abstrak: This research aimed to analyze the relation between teenager behaviors on community radio listening activity. This research goals are (1) to identify the pattern of listening activity on community radio among teenager (2) to analyze the relation between listeners characteristics and broadcast format assessment by listening community radio (3) to analyze the relation between community radio listeners pattern with the teenager behaviors, and (4) to identify the listeners expectations towards community radio. The main method of this research is quantitative approach, and qualitative approach as supporting. Research and observationshow that the teenagers who are listening to the BeTe community radio have low level of listening activity pattern. Affective level and social network were also relatively low. Related to the listeners characteristics, the research result showed that gender does not have any relation with the pattern of radio listening activity. Beside that, age, media ownership, and main activities have significant relation towards pattern of radio listening activity. To assess broadcast format variable, pshycological proximity does not have any relation significantly with the pattern of radio listening activity. In the pattern of radio listening activity variable which is consist of listening frequency, listening duration, program choices, participation on the program have significant relation with to point of view, affective, and social network of BeTe Radio listeners.
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Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable