Judul: Analisis finansial investasi peremajaan kebun teh
Abstrak: Sebagai penghasil devisa non minyak dan gas bu~ni, teh menempati urutan ketiga setelah karet dan kopi dalam kelompok kornoditas perkebunan. Sedanglcan kelompok konioditas perkebunan sendiri masih tetap memegang peranan penting dalam daftar sumber devisa negara. Masalah berat yang dihadapi oleh pengusaha perkebunan pada umurnnya dan khususnya perkebunan teh adalah gejala semakin menyempitnya marjin keuntungan. Hal ini disebabkan oleh meningkatnya biaya produksi dan harga yang cenderung terus menurun. Salah satu upaya untuk mengatasi ha1 ini adalah dengan meningkatkan produktivitas kebun. Peremajaan kebun merupakan salah satu usaha untuk menigkatkan produktivitas kebun.
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Judul: Analisis finansial pada proyek peremajaan teh  : (Studi kasus di Perkebunan Ciater, Kecamatan Jalancagak, Kabupaten Subang, Jawa Barat)
Abstrak: Tujuan dari Praktek Lapangan ini adalah untuk mengkaji kelayakan usaha penanaman investasi yang dilakukan PT Perkebunan XIII di Ciater pada proyek peremajaan teh. Dari hasil parktek lapangan dengan mengadakan analisis finansial dengan jangka waktu 25 tahun, proyek peremajaan teh layak dilaksanakan dan telah menikmati suatu keuntungan. Hal ini dapat dilihat dari kriteria penilaian yang digunakan yaitu NPV lebih besar dari nol, Net B/C ratio lebih besar dari satu dan IRR lebih besar dari tingkat bungan potongan yang digunakan. Dilihat dari MPI, memakan waktu yang relatif lama seperti umumnya komoditas pertanian. Analisis sensitivitas dilakukan untuk kenaikan biaya sebesar 10 persen, penurunan harga sebesar 10 persen serta kenaikan biaya sebesar 10 persen dan penuruna harga sebesar 10 persen secara bersamaan Pada kenaikan biaya sebesar 10 persen, proyek peremajaan masih layak dilaksanakan, demikian juga pada penurunan harga sebesar 10 persen. Pada kedua keadaan tersebut, proyek mesih memenuhi kriteria kelayakan. Sedangkan pada waktu diadakan analisis sensitivitas, kenaikan biaya sebesar dan penurunan 10 persen harga sebesar 10 persen secara bersamaan proyek sudah tidak layak lagi dilaksanakan.
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Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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