Judul: Distribusi Virus Avian Influenza (H5N1) Pada Jaringan Tubuh Itik Dengan Metode Imunohistokimia
Abstrak: Penelitian ini bertujuan untuk mengetahui distribusi virus H5N1 pada jaringan tubuh itik melalui deteksi antigen spesifik dengan menggunakan metode imunohistokimia dan mempelajari keterkaitan antara keberadaan virus AI dengan keamanan pangan daging itik sebagai ternak konsumsi. Menggunakan hewan percobaan 12 ekor jenis itik tegal yang diperoleh dari peternakan di Cirebon umur 4 bulan dan 6 bulan (telah berproduksi). Itik dikadangkan secara individual yang dibagi dalam empat kelompok perlakuan. Setiap perlakuan terdiri atas 3 ekor itik. Kelompok kontrol ditempatkan dalam ruang yang berbeda dengan kelompok perlakuan lainnya. Setelah melalui proses adaptasi, pada kelompok perlakuan I dilakukan pemberian vaksin inaktif dengan dosis 0,5 ml/ ekor pada hari ke 3. Selanjutnya pada hari ke 6 pada kelompok perlakuan I, II dan III semua itik ditantang dengan virus AI (H5N1)(FKH/IPB/Duck/NG29) dengan dosis 104 EID50 per ekor. Pada hari ke 11 semua itik telah menunjukkan gejala klinis berupa sinusitis, konjungtivitis dan diare berwarna putih. Selanjutnya dilakukan nekropsi dan pengambilan sampel organ trakea, paru-paru, jantung, hati, limpa, ginjal, pankreas, usus, otak, ovarium, isthmus otot dada dan otot paha. Dimasukkan dalam buffered Neutral Formalin (BNF) 10%. Tahap selanjutnya pembuatan blok parafin dan dilanjutkan dengan pembuatan preparat untuk pewarnaan Hemaktosilin dan Eosin/HE dan imunohistokimia. Hasil positif dinyatakan positif (+) apabila terdapat warna merah kecoklatan dan negatif jika warna tersebut tidak terdeteksi, data yang diperoleh dianalisis dengan menggunakan Kruskal Wallis.
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Judul: Kajian Persistensi dan Penularan Virus Avian Influenza di Peternakan Itik menggunakan Teknik Real Time RT-PCR
Abstrak: Highly pathogenic avian influenza (HPAI) H5N1 virus has been a major threat to poultry industry and human health in Indonesia over the past several years. The existence of backyard and free-range duck raising system has been hypothesized to play role in the disease circulation. This study investigated H5 virus infection and circulation occurring in three types of free-range duck farms in Indramayu District, West Java, Indonesia from September 2009 to March 2010. One hundred and eighty pairs of cloacal-oropharyngeal swab samples from sentinel ducks placed in six farms of the three farm types were collected every month for seven months period and screened in pools for influenza A virus. In addition to the sentinel ducks, 30 non-sentinel ducks were included at the first and the last sampling month. Of the total 648 pool samples collected for seven months, 91 pools (14%) were found influenza A positive at repeated events. The virus was more commonly found on type 1 farms followed by type 2 and 3 farms. The individual swab samples of influenza A positive pools were tested for H5 subtype. Of the total 91 pools of influenza A positive samples, 455 individual samples were tested for H5 subtype and 50 (11%) individual samples were found H5 positive. The H5 subtype was found at certain points of sampling time and was more commonly found in type 2 farms followed by type 1 and was not found on type 3 farms. The H5 virus was likely to be transmitted within duck farm but was unlikely to be maintained for a long time.
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Judul: Penyelesaian Numerik Persamaan Schrödinger untuk Molekul BaO dan SrO Menggunakan Model Potensial Morse
Abstrak: Morse potential is a potential model used to describe the behavior of a molecular vibration between atoms. This study aimed to obtain the value of the dissociation energy, the energy eigenvalues of each energy level, and describe the shape of the wave function of the molecule BaO and SrO on the vibrational state. Dissociation energy is calculated by adding the experimental dissociation energy and the ground state energy eigenvalues. Calculating the energy eigenvalues of the energy levels 0 to 4 using shooting method integrated with Numerov and Runge-Kutta method. Energy eigenvalues of each levels that calculated numerically having accuracy of 100% compared with the calculated analytically. The profil of wave functions accurately show the corresponding states and their normalized wavefunctions.
Keyword: SrO, Morse potential, energy eigenvalues, dissociation energy, BaO