Judul: Konstruksi dan Analisis Kualitas Pustaka Genom Jagung (Zea mays (L.)) untuk Sekuensing Genom Total
Abstrak: Whole genome sequencing and analysis of the maize genome is one of the ways to accelerate the increase in corn breeding and to improve the understanding of the genetic and genomic information maize. The purpose of this study is to construct genomic libraries of four maize genotypes in Indonesia (Sp010BB-3-2- 2-1-B, LK2-45, PSG59-8 (early maturing) and LBP54-50 (pulut)) and analyze the quality of the results of genomic libraries total genome sequencing. Genomic library construction performed in vitro by attaching adapters to the ends of DNA fragments. Clasterization and sequencing of genomic library in this research were conducted by using cBOT claster generation and Next Generation Sequencing HiSeq2000. The amount of base produced during the sequencing process as many as 287.1 Gbp. Cluster genomic library generated with Q values above 30 scores ranged from 80.8 – 87.9%. Base reading error rate during the sequencing process was 0.32 – 0.51%. Over all sequencing results showed that quality of the genomic library cluster was belong to the ideal category.
Keyword: Bogor Agricultural University (IPB), Next Generation Sequencing (NGS)., Whole Genome Sequencing, Genomic Library, Maize (Zea mays (L.))

Judul: Konstruksi pustaka genom dan sekuensing genom total Kakao (Theobroma cacao L.)
Abstrak: The availability of reference genome sequence of cocoa (Theobroma cacao L.) allows the full potential of the other cocoa plant genetic variation to be read and used for breeding through whole genome reading by next generation sequencing (NGS). This study describes a modification of CTAB method for extracting genomic DNA contains high polysaccharides and polyphenols, genomic library construction and whole genome sequencing using methods by Illumina. High quality of genomic DNA indicated by integrity of the molecule have been produced. Genomic libraries have been constructed were 314-485 bp in size. Base generated reach 391.93 Gbp, with genome size reach 566.9 Mbp. The quality of readings by NGS high enough with 67.9-81.3% of reading has quality value above Q30. Error rate occurs at 0.20-1.14%. Genomic library have been constructed possesses good quality and the data from sequencing were ready for further analysis.
Keyword: Bogor Agricultural University (IPB), Theobroma cacao L., next generation sequencing, genome library, DNA extraction

Judul: Studi Kasus: Patomorfologi Trakheobronkhitis Supurativa Pada Kucing (Felis Catus).
Abstrak: Trakheobronkhitis merupakan penyakit saluran respirasi yang sering terjadi pada hewan kecil, terutama anjing dan kucing. Penelitian berupa studi kasus ini bertujuan untuk mempelajari patomorfologi trakheobronkhitis supurativa pada kucing. Perubahan patologi anatomi berupa eksudat purulen pada lumen trakhea dan warna paru-paru yang tidak homogen, warna lebih gelap pada salah satu lobus, dan permukaan paru-paru lebih menonjol. Perubahan histopatologi berupa badan inklusi amfofilik-eosinofilik intranuklear yang dikelilingi oleh clear halo dan titik-titik kromatin ditepinya pada epitel kelenjar trakhea dan epitel bronkhiolus. Badan inklusi ini merupakan ciri dari FHV-1 dan adenovirus. Pada trakhea juga ditemukan koloni bakteri berbentuk coccobacillus sebagai infeksi sekunder. Bakteri tersebut diduga Bordetella sp. yang sering menginfeksi saluran respirasi kucing. Deskuamasi terjadi pada epitel trakhea dan bronkhiolus. Selain itu, ditemukan juga sel radang limfosit, makrofag, sel plasma, dan neutrofil. Pada paru-paru ditemukan lesio bronkhiolitis. Ditemukan juga bronkhopneumonia yang ditandai infiltrasi sel radang neutrofil dan sel nekrosis pada lumen bronkhiolus. Paru-paru juga mengalami emfisema dan edema pulmonum. Hiperemia dan hemoragi juga ditemukan pada kapiler paru-paru. Pada kasus ini, trakheobronkhitis supurativa diduga disebabkan oleh FHV-1, adenovirus, dan bakteri jenis Bordetella sp.
Keyword: badan inklusi amfofilik-eosinofilik intranuklear, infeksi bakteri, kucing, patomorfologi