Judul: Identification of Single Nucleotide Polymorphism (SNP) 5’UTR of Stearoyl-CoA Desaturase (SCD) in Bali Cattle
Abstrak: Sapi bali adalah salah satu sapi asli Indonesia, yang didomestikasi dari banteng (Bibos banteng). Sapi bali memiliki potensi untuk menghasilkan kualitas daging yang baik. Gen SCD adalah salah satu gen yang berperan dalam menentukan kualitas daging dengan mengubah asam lemak jenuh menjadi asam lemak tak jenuh. Tujuan dari penelitian ini adalah untuk mengidentifikasi variasi SNP 5’UTR gen SCD di sapi bali. Sebanyak 25 sampel sapi bali disekuensing menggunakan jasa 1st Base Selangor, Malaysia dan 16 sampel berhasil disekuensing. Hasil sekuensing kemudian dianalisis menggunakan program FinchTV. Hasil penelitian menunjukkan bahwa terdapat 1 SNP yaitu pada posisi g.-134A>del gen SCD pada sapi bali yang dapat dijadikan sebagai kandidat marker assited selection pada sapi bali. SNP gen SCD sapi bali pada daerah 5’UTR bersifat polimorfik.
Keyword: 5’UTR, gen SCD, sapi bali, sekuensing

Judul: The association of the single nucleotide polymorphism of Stearoyl-CoA Desaturase (SCD|HinfI) gene with meat quality and production  traits in Bali cattle
Abstrak: Bali cattle (Bos javanicus) is Indonesian native cattle which is the result of domestication of banteng. The Stearoyl-CoA Desaturase gene contributes to the regulation of fatty acids and is associated with carcass quality in Bali cattle. The SCD gene in cattle has a sequence length of 17.088 bp and is located on chromosome 26 consisting of six exons and five introns. This study was aimed to obtain the genetic diversity of the SCD|HinfI gene in beef cattle and its association with meat quality and production traits. A total of 133 blood samples were used in this study. Blood samples of 52 bali cattle were obtained from BPTU Bali Cattle Denpasar, Bali Province. In comparison, other beef cattle were used in this study i.e. Belgian-blue (n=30 cattle) and Limousin (n=14 cattle) from BPTU-HPT Padang Mangatas, Peranakan Ongole (PO) (n=30 cattle) from Village Breeding Center (VBC) Kebumen, and Wagyu (n=7 cattle) from BET Cipelang, respectively. The polymorphism of the SCD|HinfI gene was analyzed using PCR-RFLP technique. The meat characteristics in Bali cattle were analysed using ultrasonography imaging. Genotypic and allelic frequencies, heterozygosity, and Hardy-Weinberg equilibrium were analyzed using PopGen 1.32 program. The association between SCD gene SNP and meat quality and production traits was analyzed using a t-test. The SNP g.10428C>T was successfully amplified at 51 oC annealing temperature for 15 seconds with a PCR product length of 569 bp. There was homozygous genotype (CC and TT) and heterozygous genotype (CT) of SCD|HinfI gene was found in Bali cattle population with allele frequencies of 0,26 and 0,74, for C and T, respectively. In comparison, in other beef cattle (Belgian Belgian-blue, Limousin, PO, and Wagyu) only CC genotype was found with the C allele frequency of 1,00. The heterozygosity values of expectations (Ho) and observations (He) were 0,44 and 0,39, respectively. The allele frequency SCD|HinfI of Bali cattle was in Hardy-Weinberg equilibrium. The SCD gene was associated with backfat thickness marbling score,and percentage of intramuscular fat, but was not associated with growth traits. Thus the SNP g.10428C>T gen SCD could be considered as marker candidate for improving meat quality of Bali cattle.
Keyword: Bali cattle, meat quality, PCR-RFLP, SCD gene, SNP

Judul: Penggunaan Teorema Homeomorphy 2-Manifold dan Teorema Euler Poincare pada Torus 𝑇�� dan Simplicial Complex 𝐾��
Abstrak: Two dimensional topological spaces are said to be homeomorphic if they have the same topological invariant, where one of topological invariant used is an Euler characteristic. Homeomorphy 2-Manifold’s Theorem and Euler Poincare’s Theorem are used to distinguish two topological spaces. Homeomorphy 2-Manifold’s Theorem uses Euler characteristic to identify two dimensional topological spaces. Euler Poincare’s Theorem is an alternative way to find Euler characteristic with Betti number, which is topological invariant as well. The objective of this paper is to investigate the homeomorphism of torus 𝑇����� and simplicial complex 𝐾�����. Topological space torus and simplicial complex 𝐾����� have the same Euler characteristic, which is two. Based on Homeomorphy 2-Manifold’s Theorem topological space torus 𝑇����� and simplicial complex 𝐾����� is homeomorphic.
Keyword: simplicial, torus, Euler, homeomorphism, topology