Judul: Flexural behaviour of bamboo truss structure using bolts and fibers joint
Abstrak: Bambu merupakan bahan alternatif sebagai pengganti kayu yang berpotensial untuk dikembangkan sebagai konstruksi. Bambu memiliki sifat fisis dan mekanis yang sama seperti kayu. Namun, bentuk bambu yang silindris berongga dan berbuku menyebabkan masalah saat penyambungan. Tujuan penelitian ini adalah mengkaji perilaku lentur kuda-kuda bambu Andong dan Tali menggunakan sambungan baut dan ijuk pada tiga tipe kuda-kuda. Penggunaan Stucture Analysis Program (SAP) dilakukan untuk melihat dan membandingkan perilaku lentur kuda-kuda melalui program komputer dengan perilaku aktual. Hasil penelitian menunjukkan bahwa kerusakan yang secara umum terjadi pada rangka kuda-kuda sambungan baut dan tali ijuk adalah pecah, retak pada bagian titik buhul, tali kendur bahkan putus akibat adanya pergeseran elemen batang, rusaknya plat sambung dan baut geser. Struktur kuda-kuda bambu Andong sambungan baut mampu menahan beban besar dibandingkan dengan tali ijuk. Tipe kuda-kuda memiliki pengaruh terhadap kemampuan menahan beban, ketiga tipe kuda-kuda yang digunakan memiliki stabilitas yang baik dalam menahan beban. Terjadi perbedaan perilaku lentur berdasarkan nilai defleksi antara pengujian aktual dibandingkan SAP., Bamboo is an alternative material that potentially can be developed as construction. It’s caused bamboo has physical and mechanical properties are similar to wood. However, hollow and nodes were closed to be problems in jointing system. The aims of this research was assess the flexural behaviour of bamboo truss structure using bolts and fibers joint. Use of Stucture Analysis Program (SAP) was conducted to over view and compare the flexural behaviour of the trussess through a computer program and actual behavior testing. The results showed that generally the damage occurs using bolts joint were checks, shake, splits on connected plate, while the damage on fibers joints were checks, shake, slack and break of fibers. The structure of truss bamboo Andong using bolts join have the capable to hold great loads cells than fibers joint. Type of trusses have an influence on load bearing capability, all type of trusses has high stability to hold loads better. The difference of flexural behaviour has occurred between SAP and actual testing based on deflection.
Keyword: Bambu, perilaku lentur, sambungan, SAP

Judul: Cross-species bamboo grading based on the flexural properties
Abstrak: Bambu telah lama digunakan sebagai bahan konstruksi oleh masyarakat Indonesia. Pemilahan struktural bambu diperlukan sebagai landasan pembentukan standar konstruksi bambu yang memadai. Pemilahan bambu sebagai bahan konstruksi struktural dapat dilakukan melalui pemilahan visual atau pemilahan masinal. Pemilahan bambu spesisik pada suatu spesies tertentu telah dilaporkan. Penelitian ini bertujuan untuk mengevalusi kemungkinan membangun model pemilahan bambu struktural lintas spesies. Penelitian ini menguji sifat lentur bambu Ampel (Bambusa vulgaris), Andong (Gigantochloa pseudoarundinaceae), Betung (Dendrocalamus asper), dan Hitam (Gigantochloa atroviolacea). Beberapa data sifat lentur bambu Tali (Gigantochloa apus) yang telah dilakukan sebelumnya juga ditambahkan dan dianalis secara kumulatif untuk mengembangkan model pemilahan struktural bambu lintas spesies. Analisis data menggunakan regresi berpeubah boneka (dummy variable). Peubah boneka merepresentasikan pengaruh spesies. Nilai koefisien regresi bagi setiap peubah boneka berbeda secara signifikan sehingga garis tren yang berimpit tidak ditemukan, tetapi beberapa garis tren sejajar. Garis tren yang sejajar tetapi tidak berimpit menunjukkan bahwa satu persamaan linier dapat menduga nilai rata-rata sifat penentu mutu (grade determining property, GDP), lalu konstanta perlu ditambahkan untuk mempertimbangkan pengaruh spesies. Sementara itu, garis tren yang tidak sejajar dan tidak berimpit menunjukkan bahwa persamaan linier yang berbeda harus diterapkan untuk setiap spesies bambu. Penelitian ini menunjukkan spesies memiliki pengaruh yang kuat, sehingga pemilahan struktural bambu lintas spesies tidak terbukti dapat diandalkan. Oleh karena itu, penulis menyarankan untuk mempertimbangkan identifikasi spesies pada pemilahan bambu struktural.
Keyword: Bamboo, dummy variable, mechanical properties, regression analysis, structural grading, structural quality classification

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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