Judul: Pengaruh kepadatan terhadap interaksi sosial warga rumah susun apron kemayoran, Jakarta Pusat
Abstrak: DKI Jakarta menanggung jumlah penduduk yang semakin banyak dari tahun ke tahun. Jakarta Pusat dan Kemayoran merupakan wilayah kotamadya dan kecamatan dengan tingkat kepadatan penduduk paling tinggi. Kepadatan penduduk yang semakin meningkat menimbulkan permasalahan permukiman di Jakarta Pusat, khususnya di kecamatan Kemayoran. Pemerintah berupaya mengatasi masalah ini dengan membuat tempat tinggal yang menumpuk ke atas (flat), agar penduduk yang semakin padat memperoleh tempat yang layak sebagai tempat tinggal.
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Judul: Analisis Pola Spasial Urbanisasi dan Perkembangan Wilayah di Koridor Mega Urban Jakarta-Bandung.
Abstrak: Fenomena urbanisasi, suburbanisasi, dan konurbasi di koridor mega urban Jakarta-Bandung disebabkan karena pertumbuhan penduduk yang terus meningkat tiap tahunnya. Selain timbulnya masalah kependudukan, kepadatan dan pertumbuhan penduduk yang tidak terkendali dapat menimbulkan berbagai masalah sosial dan ekonomi seperti meningkatnya angka pengangguran, kemiskinan, dan ketidakmerataan. Berdasarkan hal tersebut diperlukan analisis spasial terkait pola urbanisasi Jakarta-Bandung sebagai acuan dalam pengembangan koridor mega urban Jakarta-Bandung ke depannya. Penelitian ini bertujuan untuk mengidentifikasi pola perubahan sebaran kepadatan dan pertumbuhan penduduk di koridor mega urban Jakarta-Bandung, mengidentifikasi pola sebaran indeks perkembangan wilayah di kawasan tersebut, dan zonasi wilayah. Metode yang digunakan pada penelitian ini meliputi analisis skalogram, analisis spatial clustering, dan analisis data spasial. Kepadatan penduduk di koridor mega-urban Jakarta-Bandung mengalami peningkatan dari tahun 2003 hingga tahun 2014. Pola perubahan sebaran kepadatan penduduk mengikuti jaringan jalan tol dan jalan nasional yakni Tol Cipularang dan Jalur Puncak. Di sisi lain, pertumbuhan penduduk di sepanjang koridor Jakarta-Bandung meningkat dari tahun 2003 hingga tahun 2011 lalu menurun dari tahun 2011 hingga tahun 2014. Indeks Perkembangan Kecamatan (IPK) di koridor mega urban Jakarta- Bandung juga mengalami perluasan dan peningkatan dari tahun 2003 hingga tahun 2014. Pada wilayah studi dapat dilakukan beberapa skenario pewilayahan pembangunan berdasarkan kehomogenan karakteristik wilayah.
Keyword: indeks perkembangan kecamatan, kepadatan penduduk, spatial clustering

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable