Judul: Studi Aspek Kenyamanan Ruang Pedestrian  dalam Rangka Peningkatan Efektivitas  Penggunaannya pada Kawasan Jalan M.H.  Thamrin-Jend. Sudirman Jakarta
Abstrak: Kawasan jalan M.H. Thamrin–Jend. Sudirman Jakarta, merupakan kawasan jalan yang banyak dilalui oleh pengguna jalan, termasuk pejalan kaki. Sejak awal tahun 1980 hingga awal tahun 2000, penyediaan fasilitas jalur sirkulasi di kawasan ini yaitu berupa ruang pedestrian, kurang memberikan kenyamanan bagi penggunanya. Pemikiran untuk memanusiakan lingkungan jalan M.H. Thamrin- Jend. Sudirman, secara konseptual muncul pada tahun 1981 dengan rencana penataan kawasan oleh Dinas Pertamanan. Namun baru pada tahun 2002, setelah kondisi sosial, politik dan ekonomi mulai stabil, ide untuk mengembangkan pedestrian jalan M.H. Thamrin-Jend. Sudirman kembali dimunculkan oleh Gubernur Sutiyoso hingga sekarang.
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Judul: Study of Comfort Aspects of Pedestrian  Paths in Blok M Area, South Jakarta
Abstrak: ABSTRAK SAVIRA PERMATA PUTRI. Kajian Aspek Kenyamanan Jalur Pedestrian di Kawasan Blok M, Jakarta Selatan. Dibimbing oleh HADI SUSILO ARIFIN. Kawasan Blok M merupakan pusat kegiatan ekonomi dengan mobilitas yang tinggi di Jakarta Selatan. Kawasan ini didukung oleh berbagai integrasi moda transportasi. Jalur pedestrian juga menjadi perhatian pemerintah dalam upaya mendukung kampanye transportasi hijau. Revitalisasi jalur pedestrian dilakukan dengan perbaikan secara fisik. Penelitian ini bertujuan untuk mengevaluasi jalur pedestrian guna mengetahui kualitas dan tingkat kenyamanan dari hasil revitalisasi yang telah dilakukan. Penelitian ini menggunakan tiga analisis, yaitu Pedestrian Environmental Quality Index, Gap analysis, dan Importance Performance Analysis. Hasil penelitian menunjukkan kualitas jalur pedestrian di segmen Panglima Polim Raya, Melawai Raya, dan Iskandarsyah Raya terbagi menjadi kelas I – ideal (skor 81-100), II – baik (skor 61-80), III – rata-rata (skor 41-60), dan IV – minim (skor 21-40). Pada tiga segmen jalan tersebut didominasi oleh kelas II. Tingkat kesenjangan kenyamanan antara kondisi aktual dan ideal persepsi pengguna menunjukkan bahwa 10-11 sub-faktor memiliki nilai gap di atas rata-rata pada tiap segmen pengamatan. Terdapat enam sub-faktor yang menjadi prioritas utama untuk diperbaiki, yaitu pohon peneduh, ketersediaan dan kondisi dari street furniture, ketersediaan dan kondisi fasilitas disabilitas, posisi street furniture dan utilitas, serta kualitas udara., ABSTRACT SAVIRA PERMATA PUTRI. Study of Comfort Aspects of Pedestrian Paths in Blok M Area, South Jakarta. Supervised by HADI SUSILO ARIFIN. Blok M area is a center of economic activities with high mobility in Southern part of Jakarta. This area is supported by various integrations of transportation modes. Pedestrian paths area also a concern of the government in an effort to support green transportation campaign. Revitalization of the pedestrian path is carried out with physical improvements. The objective of study is to evaluate pedestrian paths in order to determine the quality and comfort level of the existing revitalization results. This study uses three analyses, in the form of Pedestrian Environmental Quality Index, Gap analysis, and Importance Performance Analysis. The results showed that the quality of the pedestrian paths in Panglima Polim Raya, Melawai Raya, and Iskandarsyah Raya segments was divided into class I – ideal (score 81-100), II – good (score 61-80), III – average (score 41-60), and IV – minimum (score 21-40). The three path segments are dominated by class II. The level of the comfort factor gap between the actual and ideal conditions user perceptions, showed that 10-11 sub-factors have a gap value above the average in each observation segment. There are six sub-factor main priority to improvement, there are shade trees, availability and condition of street furniture, availability and condition of disabled facilities, position of street furniture and utilities, and air quality.
Keyword: actual comfort, ideal comfort, IPA, PEQI, revitalization

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.