Judul: Aplikasi Hormon Pertumbuhan Rekombinan melalui Perendaman untuk Memacu Pertumbuhan Benih Ikan Gurami (Osphronemus goramy)
Abstrak: This study aimed to determine the immersion frequency of recombinant common carp (CcGH) and giant gourami (OgGH) growth hormone in the growth stimulation of giant gourami juvenile. A total of 50 larvae at second day after first feeding, were immersed in 30 ppt salt water for 2 minutes, then transferred into 200 mL of 9 ppt saline water containing 30 mg/L CcGH in inclusion bodies form. Immersion was performed for 60 minutes with a frequency of 2, 3, 4 and 5 times at interval 7 days. The results showed that the highest (p<0.05) average body weight of giant gourami juvenile (2.232±0.190 g) was obtained at 4 times immersion treatment. The total dosage of 4 times immersion (120 mg/L OgGH) from the first experiment was then used in the second experiment to determine whether immersion frequency could be minimized. Fish larvae were immersed once a week, twice a week, and once a week for 2 weeks. The results showed that the highest (p<0.05) biomass (5482.58±110.70 g), and survival rate (87.38±8.84%) were obtained by once immersion time. Hence, both CcGH and OgGH could improve growth of giant gourami juvenile. The IGF-I mRNA expression level was increased significantly at 24 hours after OgGH immersion, and this suggested that IGF-I was involved in growth stimulation. Lower plasma cortisol levels in OgGH-treated fish after 180 minutes may also plays important role in growth induction. Thus, immersion of juvenile once a week in 120 mg/L OgGH solution can be applied to enhance the growth of giant gourami with a high survival rate.
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Judul: Respons benih ikan gurame (Osphronemus goramy) yang diberi hormon pertumbuhan rekombinan melalui oral pada dosis berbeda
Abstrak: This study was aimed to evaluate the response of giant gourami juvenile on oral administration of recombinant giant grouper growth hormone (rElGH). A total of 45 juvenile were fed with rElGH coated by hydropropylmethyl cellulose phthalate (HP55) and mix with commercial diet (35% protein content). Feeding fish with diet containing four different doses of rElGH (0 mg/kg feed, 0.3 mg/kg feed, 3.0 mg/kg feed, and 30.0 mg/kg feed) was performed in twice a week for 2 month. Fish were then maintained for 1 month without rElGH treatment. The results showed that biomass harvest in treatment 3.0 mg/kg (1,376.0±38.3 g) and 30.0 mg/kg (1,362.2±78.5 g) was similar, and the two treatments were 46.76% and 44.28% higher compared to control (977.6±96.7 g), respectively. In addition, rElGH treatment increased appetite, while artificial feed conversion ratio (1.18) was lower (P<0.05) by 23.72% compared to that of control (1.46). Oral administration of rElGH also increased hepatosomatic index (16.12%) and protein content (17%), while lipid content (21%) decreased compared to control. Similar in fish survival at end of experiment, insulin-like growth factor-1 and growth hormone receptor-1 gene expression levels in brain at 24 hours after feeding in rElGH treatment and control were obtained. Thus, orally rElGH administration was effective to be applied to enhance the growth of giant gourami juvenile.
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Judul: Penentuan Nilai Eigen dan Vektor Eigen dari Matriks Tridiagonal 2-Toeplitz dengan Pendekatan Polinomial Chebyshev
Abstrak: The eigenvalues and eigenvectors of a matrix can be determined by finding its characteristic polynomials. The characteristic polynomials of a tridiagonal 2-Toeplitz matrix is shown to be closely connected to polynomials which satisfy the Chebyshev recurrence relationship. If the order of the matrix is odd, then the eigenvalues are found explicitly in terms of the Chebyshev zeros and the eigenvectors are found in terms of the polynomials satisfying the recurrence relationship. For even ordered matrices, the situation is more complicated. The problem in these cases is that although the Chebyshev recurrence formula is still applied, its initial values are not generating Chebyshev polynomials
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