Judul: Flora Mangrove Pulau Belitung Provinsi Kepulauan Bangka Belitung
Abstrak: Pulau Belitung merupakan pulau yang berada di antara Pulau Sumatra dan Kalimantan. Pulau Belitung dipengaruhi oleh perairan laut dari Selat Karimata (timur), Selat Gaspar (barat), Laut Natuna Selatan (utara) dan Laut Jawa (selatan). Pengaruh perairan laut terhadap daratan menyebabkan terbentuknya zona pasang surut yang ditumbuhi oleh vegetasi mangrove (bakau). Data taksonomi dan ekologi flora mangrove di Pulau Belitung belum tersedia, terutama data keanekaragaman jenis dan zonasi flora mangrove. Penelitian ini bertujuan untuk mendeskripsikan keanekaragaman dan menganalisis zonasi flora mangrove di Pulau Belitung. Data ini sangat diperlukan sebagai data dasar yang bisa digunakan untuk konservasi atau pengembangan lokasi tersebut. Penelitian ini menggunakan metode jelajah untuk mengeksplorasi keanekaragaman jenis dan metode transek garis untuk menganalisis zonasi serta interaksi faktor lingkungan dengan flora mangrove di Pulau Belitung. Beberapa lokasi untuk pengumpulan data keanekaragaman adalah di Pantai Kuale Tambak (Desa Sukamandi, Kecamatan Damar), Pantai Asam (Desa Aik Kelik, Kecamatan Damar), Kawasan Belitung Mangrove Park (Desa Juru Seberang, Kecamatan Tanjung Pandan), Sungai Manggar (Desa Mekar Jaya, Kecamatan Manggar), Pantai Sentigi (Desa Dukong, Kecamatan Simpang Pesak), Pantai Sabong (Desa Tanjung Rusa, Kecamatan Membalong) dan Pantai Kembiri (Desa Kembiri, Kecamatan Membalong). Analisis zonasi flora mangrove dilakukan di Pantai Asam (utara), Pantai Kembiri (selatan) dan Pantai Kuale Tambak (timur). Flora mangrove di Pulau Belitung yang ditemukan berjumlah 12 suku, 16 marga dan 24 jenis. Lokasi dengan jumlah jenis terbanyak adalah Pantai Kembiri, sebanyak 18 jenis. Suku terbesar yang mendominasi flora mangrove adalah Rhizophoraceae. Jenis yang ditemukan dari suku Rhizophoraceae sebanyak 8 jenis. Bruguiera dan Rhizophora merupakan dua marga dengan jenis terbanyak. Jenis tumbuhan penyusun flora mangrove Pulau Belitung yang ditemukan adalah Acanthus ilicifolius, Avicennia alba, Avicennia marina, Finlaysonia obovata, Nypa fruticans, Lumnitzera littorea, Lumnitzera racemosa, Excoecaria agallocha, Pemphis acidula, Sonneratia alba, Heritiera littoralis, Xylocarpus granatum, Aegiceras corniculatum, Acrostichum auereum, Acrostichum speciosum, Bruguiera cylindrica, Bruguiera gymnorhiza, Bruguiera parviflora, Bruguiera sexangula, Ceriops tagal, Rhizophora apiculata, Rhizophora mucronata, Rhizophora stylosa dan Scyphiphora hydrophyllacea. Jenis flora mangrove dengan kelimpahan tertinggi adalah Rhizophora apiculata. Zonasi flora mangrove pada setiap lokasi penelitian di Pulau Belitung menunjukkan adanya pola zonasi yang berbeda. Vegetasi mangrove di Pantai Kulae Tambak berada di belakang vegetasi Casuarina equisetifolia yang merupakan tumbuhan hutan pantai. Vegetasi mangrove di Pantai Asam dan Kembiri berhadapan langsung dengan laut lepas. Perbedaan pola zonasi ini disebabkan oleh topografi lokal, kondisi substrat dan faktor salinitas air di wilayah mangrove Pulau Belitung., Belitung Island is located between the Sumatra and Borneo islands. Belitung Island is influenced by the sea waters of the Karimata Strait (east), Gaspar Strait (west), South Natuna Sea (north) and Java Sea (south). The influence of marine waters on land causes the formation of tidal zones overgrown with mangrove vegetation (bakau). Taxonomic and ecological data of mangrove flora in Belitung Island are not yet available, especially data on species diversity and zonation of mangrove flora. This study aims to reveal the diversity and analyze the zonation of mangrove flora in Belitung Island. This data is indispensable as basic data that can be used for conservation purposes or to development of the site. This study was conducted using a cruising method to explore the diversity of species
Keyword: Belitung, diversity, mangrove flora, Rhizophoraceae, zonation

Judul: Macrozoobenthos Diversity on the Mangrove  Ecosystem in Coast of Kaliwlingi Village, Brebes, Central Java
Abstrak: Ekosistem mangrove merupakan habitat flora dan fauna di daerah pantai dan berfungsi menopang hidup biota perairan salah satunya bentos. Makrozoobentos merupakan organisme yang hidup di dasar perairan dan dipengaruhi oleh kualitas perairan. Desa Kaliwlingi, Brebes, Jawa tengah memiliki vegetasi mangrove yang luas yang mengalami alih fungsi hutan menjadi tambak yang massif sehingga menyebabkan rusaknya ekosistem dan perlunya penelitian terkait makrozoobentos di kawasan mangrove. Tujuan dari penelitian ini adalah mengidentifikasi dan menganalisis keanekaragaman makrozoobentos di ekosistem mangrove Desa Kaliwlingi, Brebes, Jawa Tengah yang dilaksanakan pada bulan November 2022. Metode yang digunakan yaitu metode plot transek garis. Data yang dikumpulkan dengan melakukan survei dan pengamatan langsung. Hasil penelitian menunjukkan bahwa kerapatan mangrove tergolong jarang dengan kisaran 70 ind/1000 m2 dan keanekaragaman makrozoobentos tergolong rendah dengan kisaran 1,62. Jenis makrozoobentos yang ditemukan 7 jenis yaitu Cassidula vespertilionis, Cerithideopsilla cingulata, Cheilonereis sp., Polinices incei, Puperita sp., Sesarma sp., dan Telescopium telescopium. Jenis mangrove terdapat 3 jenis yaitu Avicennia marina, Avicennia alba dan Rhizophora mucronata. Substrat lokasi penelitian didominasi jenis pasir halus. Hasil analisis CA menunjukkan Makrozoobentos jenis Cerithideopsilla cingulata dan Telescopium telescopium berasosiasi dengan mangrove jenis Rhizophora mucronata dengan kerapatan terendah dan tertinggi serta Avicennia alba dengan kerapatan rendah; Cheilonereis sp., Cassidula vespertilionis, dan Sesarma sp.; Polinices incei berasosiasi dengan Avicennia alba kerapatan terendah; sedangkan Puperita sp. tidak berasosiasi dengan jenis mangrove yang ada di lokasi penelitian., The mangrove ecosystem is a habitat for flora and fauna in coastal areas and functions to support the life of aquatic biota, one of which is the benthic. Macrozoobenthos are organisms that live at the bottom of the waters and are affected by water quality. Kaliwlingi Village, Brebes, Central Java has extensive mangrove vegetation which has experienced a conversion of forest functions into massive ponds causing damage to the ecosystem and its is necessary to carry out research related to macrozoobenthos in the mangrove area. The purpose of this study was to identify and analyze the capture of macrozoobenthos in the mangrove ecosystem of Kaliwlingi Village, Brebes, Central Java and was conducted in November 2022. The method used is the line transect plot method. Data collected by conducting surveys and direct observation. The results showed that the density of mangroves was relatively rare with a range of 70 ind/1000 m2 and macrozoobenthic habitats were classified as low with a range of 1.62. There were 7 types of macrozoobenthos found, such as Cassidula vespertilionis, Cerithideopsilla cingulata, Cheilonereis sp., Polinices incei, Puperita sp., Sesarma sp., and Telescopium telescopium. There are 3 types of mangroves namely Avicennia marina, Avicennia alba and Rhizophora mucronata. The substrate of the research location is dominated by fine sand. CA analysis results showed that the macrozoobenthos types Cerithideopsilla cingulata and Telescopium telescopium were associated with mangrove species Rhizophora mucronata with the lowest and highest densities and Avicennia alba with low densities; Cheilonereis sp., Cassidula vespertilionis, and Sesarma sp.; Polinices incei associated with Avicennia alba lowest density; while Puperita sp. not associated with mangrove species in the study area.
Keyword: Association, Density, Macrozoobentos, Mangrove

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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