Judul: Study on the Advantages of Vegetation along the Commuter Line Railway Corridor in the City of Bogor
Abstrak: Sempadan jalur KRL Commuter Line merupakan koridor pembatas antara kegiatan manusia dengan perkeretaapian. Bangunan permukiman memenuhi area sempadan yang letaknya berdekatan dengan rel. Bangunan tersebut menimbulkan permasalahan karena fungsi sempadan tidak optimal. Vegetasi sebagai elemen sempadan diharapkan mampu mengatasi permasalahan sekaligus memanfaatkan fungsi ekologisnya. Penelitian bertujuan mengevaluasi dan menganalisis sempadan dan fungsi vegetasi di dalamnya, serta memberikan rekomendasi penanaman vegetasi pada sempadan jalur KRL sesuai dengan fungsi ekologis. Penelitian menggunakan metode deskriptif kuantitatif yang berfokus pada kondisi sempadan serta fungsi ekologis vegetasi sebagai kontrol polusi dan peredam bising. Analisis fungsi ekologis menggunakan regresi linear sederhana dan i-Tree Canopy. Sempadan rel KRL Commuter Line di Kota Bogor tidak sesuai dengan fungsi dan kebijakan. Permukiman mendominasi sempadan sehingga ruang terbuka hijau yang seharusnya digunakan sebagai pembatas tidak berfungsi secara optimal. Tanaman yang mendominasi sempadan merupakan tanaman invasif yang tidak beraturan dan juga kurang efektif dalam kontrol polusi dan peredam bising. Fungsi tanaman sebagai peredam bising berkorelasi positif dan kuat dengan nilai reduksi kebisingan sebesar 95,8% (kerapatan) dan 93,2% (dominan). Sempadan dengan dominasi dan kerapatan yang tinggi dapat meredam tingkat kebisingan lebih baik. Tanaman sebagai pengendali polusi hanya dapat menyerap dan menyimpan 0,8% emisi karbon KRL. Fungsi sempadan dapat ditingkatkan melalui penataan vegetasi dengan pemilihan tanaman yang sesuai., KRL commuter line borders are the corridor between human activities and railways. Residential buildings fill the border area, which is located close to the railroad. These buildings cause problems because the function of the border is not optimal. Vegetation as a border element is expected to overcome the problem while utilizing its ecological function. The research aims to evaluate and analyze the boundary and the function of vegetation within it and provide recommendations for planting vegetation on KRL commuter line boundaries by ecological functions. The research uses a quantitative descriptive method that focuses on the condition of the boundary and the ecological function of vegetation as pollution control and noise cancellation. The ecological function analysis used simple linear regression and i-Tree Canopy. KRL Commuter Line rail boundaries in Bogor City are not by functions and policies. Settlements dominate the border so that green open space which should be used as a barrier does not function optimally. Plants that dominate the border are invasive plants that are irregular and also less effective in pollution control and noise cancellation. The function of plants as noise absorbers is positively and strongly correlated with noise reduction values; 95,8% (density) and 93,2% (dominance). Borders with high dominance and density can reduce noise levels better. Plants as pollution control can only absorb and store 0,8% of commuter carbon emissions. The function of the border can be improved through the arrangement of vegetation with the selection of appropriate plants.
Keyword: green open space, vegetation, ecological functions, railway border

Judul: Evaluasi jalur hijau jalan sebagai penyangga lingkungan sekitarnya dan keselamatan pengguna jalan bebas hambatan jagorawi
Abstrak: The traffic problems have been increasing in recent few years. In many big cities, the volume of vehicle causes air pollutions and noises that are disturbing the comfortably of men activities. Highway is the most contributing factor in traffic problems. From many solutions, the greenery in highway is the one of solutions against negative effects of traffic as environment buffer, especially for communities and for supporting users safety. The objectives of this study are to evaluate roadside greenery in Jagorawi Highway as a buffer and for supporting of users safety in three major functions, there are air pollutants reduction, noises abatement, and space barrier. Fieldwork researches were conducted onto three segments road: Bogor’s - Ciawi’s tollgates to Sentul’s ramp as 1st segment (± 15,4 km length); Sentul’s ramp to Cimanggis’s ramp as 2nd segment (± 13,5 km length); and Cimanggis’s ramp to Taman Mini’s tollgate as 3rd segment (± 15 km length). Every segment was divided into two sections: East side and West side. The comparative method was used to measure suitability and compatibility plants in roadside greenery in Jagorawi according to the regulations and principles of landscape architecture science. The comparative method result that all segments and sections on fieldwork indicate that plants selection, structures, patterns and configurations were not suitable and compatible enough for supporting the three major functions of road greenery (assessment ranged from bad to moderate grades). The greenery at first segment achieves moderate grade (44,26% requirements fulfilled) in air pollutants reduction, bad to moderate grade (32,67% - 41,67% requirements fulfilled) in noises abatement, moderate grade (56,00% - 57,33% requirements fulfilled) in space barrier function and moderate grade (56,00% - 58,17% requirements fulfilled) in aesthetic function. The second segment achieves moderate grade (47,54% - 50,32% requirements fulfilled) in air pollutants reduction, bad grade (30,0% requirements fulfilled) in noises abatement, moderate grade (57,69% - 58,85% requirements fulfilled) in space barrier function and moderate grade (57,50% - 58,46% requirements fulfilled) in aesthetic function. The third segment achieves moderate grade (49,35% - 50,74% requirements fulfilled) in air pollutants reduction, moderate to good grade (59,33% - 69,67% requirements fulfilled) in noises abatement, good grade (62,83% - 69,67% requirements fulfilled) in space barrier function and moderate grade (59,42% requirements fulfilled) in aesthetic function. According to the above analysis result, the study proposes a concept of appropriate plants selecting, according its structure and performance and so pattern and configuration to achieve the effectiveness of roadside greenery as environment buffer and supporting the safety in Jagorawi Highway.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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