Judul: Pekerja Anak-Anak di Pedesaan (Peranan dan Dampak Anak Bekerja pada Rumahtangga Industri Kecil Sandal : Studi Kasus di Desa Mekar Jaya, Kecamatan Ciomas, Kabupaten Bogor, Jawa Barat)
Abstrak: Tujuan dari penelitian ini adalah untuk mempelajari : (1) Faktor pendorong dan penarik yang menyebabkan keterlibatan anak-anak dalam mencari nafkah. (2)Peranan anak-anak yang bekerja : apakah mereka mempunyai peranan penting dalam mempunyai peranan penting dalam membantu meningkatkan pendapatan rumahtangga dan (3) Mengetahui dampak (positif dan negatif) yang ditimbulkan akibat keikutsertaan anak-anak dalam mencari nafkah.
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Judul: Kondisi, Motivasi Kerja dan Keuntungan yang Diberikan Pekerja Anak pada Industri Kecil (Kasus Pekerja Anak pada Industri Sandal di Desa Mulyaharja, Kecamatan Bogor Selatan, Kota Bogor, Propinsi Jawa Barat)
Abstrak: Keterlibatan pekerja anak pada industri kecil membawa pengaruh yang besar haik terhadap diri mereka sendiri maupun industri kecil tempat dia bekerja tersebut. Penelitian ini bertujuan melihat bagaimana kondisi yang dialami pekerja anak, menelaah rnengenai motivasi kerja mereka baik motivasi awal maupun bertahan serta mengetahui apa yang menjadi keuntungan dan seberapa besar keuntungan penggunaan pekerja anak tersebut pada industri kecil.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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