Judul: Visualization of Amyloidopathy on Renal Mice After Immunized by Amyloid Beta-42
Abstrak: Penyakit Alzheimer merupakan jenis demensia berupa gangguan neurodegeneratif progresif pada lansia, yang ditandai adanya senile plaque dan neurofibrillary tangles di otak. Ginjal merupakan organ vital yang berpotensi mengalami kerusakan akibat retensi amyloid beta-42 (Aβ42). Studi ini dilakukan untuk mengidentifikasi amyloidopathy pada jaringan ginjal mencit yang terbagi menjadi 2 kelompok yaitu mencit yang diimunisasi Aβ42 (n=6) dan kontrol (n=8). Pengamatan histopatologis jaringan ginjal dilakukan dengan pewarnaan Hematoksilin Eosin. Temuan lesio dianalisis secara deskriptif dan semikuantitatif. Berdasarkan pengamatan yang dilakukan, ditemukan lesio patologis peradangan interstisial dengan sel peradangan limfosit, sel plasma, dan makrofag, serta kongesti dan hemoragi pada organ ginjal mencit perlakuan maupun kontrol. Organ ginjal mencit perlakuan yang diamati tidak menunjukkan adanya lesio sklerosis glomerulus, maupun fibrosis interstisial dan atrofi tubulus. Temuan ini diharapkan dapat melengkapi informasi mengenai penggunaan mencit sebagai hewan model penyakit Alzheimer serta membantu dalam pengembangan kit diagnostik. Kata kunci: amiloidosis, ginjal, histopatologi, mencit, penyakit alzheimer, Alzheimer’s disease is a type of dementia in the form of a progressive neurodegenerative disorder that happens in the old age, marked with the existence of senile plaque and neurofibrillary tangles in the brain. Kidneys are the vital organs which may have potential to get affected by the brain damage that is caused by amyloid beta-42 (Aβ42) retention. The aim of this study was to identify the amyloidopathy on mice renal tissues which is divided into two groups; the immunized mice by Aβ42 (n=6), and controls (n=8). Histopathological observations of renal tissue were carried out by using Hematoxylin Eosin staining. The lesion findings were then analyzed descriptively and semiquantitatively. Based on the observations, pathological lesions of interstitial inflammation that were filled with lymphocytes, plasma cells, and macrophages inflammatory cells, congestion, and hemorrhage were also identified in both treated and control mice renal tissues. The observed renal organs on the treated mice did not show glomerular sclerosis lesions, nor interstitial fibrosis and tubular atrophy. These findings are expected to complete the information regarding the use of mice as model organism of Alzheimer’s disease (AD) and assist in developing a diagnostic kit for AD. Keywords: alzheimer’s disease, amyloidosis, histopathology, kidneys, mice.
Keyword: amiloidosis, ginjal, histopatologi, mencit, penyakit alzheimer, alzheimer's disease, amyloidosis, histopathology, kidneys, mice

Judul: Study of Amyloidopathy on Mice Liver after Immunization of Amyloid Beta 42 (Aβ42) as a Marker of Alzheimer's Disease
Abstrak: Peptida amiloid beta 42 (Aβ42) dikenal sebagai biomarker dini penyakit Alzheimer. Antibodi Aβ42 menjadi bahan antibodi spesifik atau antibodi monoklonal yang dapat dijadikan kit diagnostik penyakit Alzheimer berbasis ELISA. Antibodi diproduksi di hewan mencit melalui teknik imunisasi amiloid sehingga didapatkan antibodi monoklonal dari organ limpa pada mencit. Studi ini dilakukan untuk mempelajari amyloidopathy pada organ hati mencit pascaimunisasi antibodi amiloid beta 42 (Aβ42) sebagai marka penyakit Alzheimer. Studi ini menggunakan 20 ekor mencit lalu dibagi menjadi dua kelompok. Kelompok yang diimunisasi dengan Aβ42 dan kelompok kontrol. Mencit perlakuan diberikan vaksin dengan titer 15.000pg/mL dan diinjeksikan 100l/ekor melalui rute subkutan. Pengamatan histopatologi organ hati menggunakan pewarnaan Hematoksilin Eosin. Temuan lesio dianalisis menggunakan analisis deskriptif dan semikuantitatif tipe ordinal. Hasil pengamatan menunjukkan bahwa terdapat lesio infiltrasi sel radang dan nekrosis pada mencit perlakuan. Hasil pengamatan studi ini diharapkan dapat melengkapi informasi terkait efek dari imunisasi Aβ42 terhadap organ hati mencit sebagai hewan model., Amyloid peptide beta 42 (Aβ42) is known as an early biomarker of Alzheimer's disease. Antibody Aβ42 is a specific antibody or a monoclonal antibody that can be used as an ELISA-based Alzheimer's disease diagnostic kit. Antibodies are produced in mice through the amyloid immunization technique; monoclonal antibodies will be obtained from the spleen organ in mice. This study was conducted to study amyloidopathy in the liver of mice after immunization with amyloid beta 42 (Aβ42) antibody as a marker of Alzheimer's disease. This study used 20 mice and divided them into two groups. The group immunized with Aβ42 and the control group. This study uses 15.000pg/ml vaccine titter and injected to 10 mice via the subcutaneous route with 100l/mice dose. Histopathological observations of liver organs using Haematoxylin Eosin staining and the lesion was then analysed using ordinal type descriptive and semi-quantitative analysis. The results showed that there were inflammatory cell infiltration lesions and necrosis in immunized mice. The results of this study are expected to provide information regarding the effects of Aβ42 immunization on the liver of mice as animal models.
Keyword: Alzherimer's disease, amyloidosis, hystopathology, liver, mice

Judul: Identifikasi Fluktuasi Ketinggian Tropopause di Kawasan Pasifik Tropis
Abstrak: The 30-years (January 1981 – December 2010) ERA-Interim data issued by ECMWF (Europe Center for Medium Range Weather Forecasting) is used for identifying tropopause height fluctuation in tropical Pacific region. Principle component and spectral analysis is done in order to identify the main pattern of tropopause height fluctuation as generator in the region. The result has shown that the predominant fluctuation trigger is periodical 45-month ENSO. Meanwhile insolation fluctuation due to earth orbital position has placed second with 12- month period after ENSO occurrence. The 20-month oscillation period of QBO (Quasi Biennial Oscillation), periodical 6-month variation of SAO (Semi Annual Oscillation), and 6-month period of MJO (Madden Jullian Oscillation) give weaker influence on tropopause height fluctuation in the region than that of ENSO and insolation. Eigen vector distribution from principle component shows that these five oscillations are very influential and dominant in tropical west Pacific as response of interaction of the five oscillations with Walker circulation in tropical Pacific.
Keyword: Bogor Agricultural University (IPB), Spectral, Walker circulation, Tropopause height fluctuation, Principle component analysis