Judul: The Erythrocyte Profiles in Pregnant Friesian Holstein Cows after Administered Avian Influenza H5N1 Vaccine
Abstrak: Penelitian ini bertujuan untuk mempelajari profil parameter eritrosit pada induk sapi bunting yang divaksin dengan vaksin AI H5N1. Sebanyak sembilan ekor induk sapi FH bunting trimester akhir, sehat secara klinis digunakan dalam penelitian ini. Induk sapi dibagi ke dalam tiga kelompok (n-3 ekor), yaitu kelompok control (tidak diberi imunomodulator, antigen AI H5N1, dan vaksin AI H5N1 inaktif), kelompok perlakuan 1 (tidak diberi imunomodulator, diberi antigen AI H5N1, dan vaksin AI H5N1 inaktif), dan kelompok perlakuan 2 (diberi imudomodulator, antigen AI H5N1, dan vaksin AI H5N1 inaktif). Imunomodulator (ribonucleotide polypeptide) diberikan secara oral dengan dosis 0,1 mg/kgBB tiga hari berturut-turut. Antigen H5N1 diberikan secara intravena selama tiga hari berturut-turut dengan dosis 104 HAU/ekor. Vaksin AI H5N1 inaktif plus adjuvant diberikan dua kali, masing-masing sebanyak 5 dosis/ekor secara subkutan, dengan interval waktu antar vaksinasi selama dua minggu. Pengambilan sampel darah dilakukan melalui vena coccygea pada saat sebelum vaksinasi pertama, 2 minggu setelah vaksinasi pertama, 2 minggu setelah vaksinasi kedua, satu minggu setelah vaksinasi ketiga, dan 2 minggu setelah vaksinasi ketiga untuk dianalisis terhadap jumlah eritrosit, nilai hematokrit, kadar hemoglobin, dan indeks eritrosit. Hasil pengamatan menujukkan bahwa tidak ada perbedaan nyata pada jumlah eritrosit, nilai hematokrit, kadar hemoglobin, nilai MCV, MCH, dan MCHC pada induk sapi bunting setelah vaksinasi, baik antar perlakuan maupun antar waktu pengamatan (P>0,05). Vaksinasi menggunakan vaksin Avian Influenza H5N1 inaktif pada induk sapi Friesian Holstein bunting trimester akhir tidak berpengaruh pada nilai parameter eritrosit., The objective of this experiment was to study the erythrocytes profile in pregnant Friesian Holstein (FH) cows after administered by Avian Influenza H5N1 vaccine. This experiment used nine pregnant FH cows in the last trimester of pregnancy. The cows were divided into three groups that consist of three cows for each group which are the control group (no imunomodulator, no AI H5N1 antigen, and no AI H5NI vaccine), group A (no immunomodulator, but administered with AI H5N1 antigen and AI H5N1 vaccine), and group B (administered with immunomodulator, AI H5N1 antigen, and AI H5N1 vaccine). Immunomodulator (ribonucleotide polypeptide) was administered orally with 0.1 mg/body weight dose. Antigen of inactive H5N1 was given by intravenous administration for consecutively in three times with 104 HAU/each cow. Avian influenza H5N1 inactive commercial vaccine was subcutaneously administered twice, with 5 doses/each cow in 2 weeks of the time interval between vaccination. The blood collection were taken from coccygeal vein before the first vaccination, two weeks after the first vaccination, two weeks after the second vaccination, one week and two weeks after the third vaccination to be analyzed for the number of erythrocytes, haematocrit, haemoglobin, Mean Corpuscular Volume, Mean Corpuscular Haemoglobin, and Mean Corpuscular Haemoglobin Concentration. The result showed that the pregnant cow’s erythrocyte parameter were not significantly different between treatments and also between the time of examination (P>0.05). In conclusion, the administration of Avian influenza H5N1 vaccine to the last trimester of pregnancy Friesian Holstein cows didn’t affect the erythrocyte parameter profiles.
Keyword: Flu Burung, Sapi Bunting, Vaksin AI H5N1, Parameter Eritrosit

Judul: Clinical Profile of Pregnant Cattle Frisien Holstein After Giving Avian Influenza H5N1 Vaccine
Abstrak: Penelitian ini bertujuan untuk mengamati profil klinis induk sapi perah Friesian Holstein (FH) bunting yang divaksin dengan vaksin Avian Influenza H5N1 melalui pengamatan suhu tubuh, frekuensi respirasi dan pulsus. Sebanyak sembilan ekor induk sapi Friesian Holstein (FH) bunting trimester akhir dibagi ke dalam tiga kelompok, yaitu kelompok kontrol (3 ekor, tidak diberi imunomodulator, antigen AI H5N1 dan vaksin AI H5N1 inaktif), kelompok A (3 ekor, tidak diberi imunomodulator, diberi antigen AI H5N1, dan divaksin AI H5N1 inaktif), dan kelompok B (3 ekor, diberi imunomodulator, antigen anti AI H5N1, dan vaksin AI H5N1 inaktif). Induk sapi diberikan imunomodulator dengan rute oral selama tiga hari berturut-turut sebelum vaksinasi dengan dosis 0,1 ml/kgBB. Imunomodulator yang digunakan berjenis polipeptida ribonukleotida. Setelah itu induk sapi kelompok A dan B disuntik dengan antigen (Ag) H5N1 in-aktif tanpa adjuvan secara intra-vena selama tiga hari berturut-turut dengan dosis 104 HAU/ekor. Selanjutnya induk sapi A dan B divaksin menggunakan vaksin AI H5N1 in-aktif komersial sebanyak 5 dosis unggas/ekor secara subkutan. Vaksin diberikan sebanyak tiga kali, masingmasing dengan interval waktu antar vaksinasi dua minggu. Parameter pemeriksaan fisik yang diamati meliputi suhu tubuh, frekuensi pulsus dan frekuensi respirasi. Pemeriksaan fisik dilakukan sebelum vaksinasi pertama, 3 hari berturut-turut setelah vaksinasi pertama, kedua dan ketiga (hasil angkaangka). Hasil pemeriksaan suhu tubuh, frekuensi respirasi dan pulsus kelompok A dan B pada vaksinasi I, II, dan III masih didalam kisaran normal. Pemberian vaksin H5N1 inaktif pada induk sapi bunting Friesian Holstein tidak berpengaruh negatif terhadap status kesehatan hewan tersebut., This study aims to observe the clinical profile of Friesian Holstein (FH) pregnant dairy cows vaccinated with Avian Influenza H5N1 vaccine through observations of body temperature, respiration frequency and pulsus. A total of nine final trimester pregnant Friesian Holstein (FH) cows were divided into three groups, namely the control group (3 cows, not given immunomodulators, AI H5N1 antigen and inactive H5N1 AI vaccine), group A (3 cows, not given immunomodulators, were given AI H5N1 antigens, and inactivated AI H5N1 vaccines), and group B (3 animals, were given immunomodulators, H5N1 anti AI antigens, and inactivated AI H5N1 vaccines). Cows were given immunomodulators by oral route for three consecutive days before vaccination at a dose of 0.1 ml/kgBB. The immunomodulator used is a ribonucleotide polypeptide. After that, cows in groups A and B were injected with in-active H5N1 antigen (Ag) without adjuvant intravenously for three consecutive days at a dose of 104 HAU/head. Furthermore, cows A and B were vaccinated using the commercial in-active H5N1 AI vaccine as many as 5 doses of poultry/head subcutaneously. The vaccine is given three times, each with a time interval between vaccinations of two weeks. Physical examination parameters observed included body temperature, pulse rate and respiration rate. Physical examination was carried out before the first vaccination, 3 consecutive days after the first, second and third vaccinations (results figures). The results of examinations of body temperature, respiration frequency and pulses of groups A and B in vaccinations I, II, and III were still in the normal range. The conclusion of this study was that the administration of inactivated H5N1 vaccine to pregnant Friesian Holstein cows
Keyword: Avian Influenza, imunomodulator, Frisien Holstein cow, H5N1 vaccination

Judul: Penyusunan Overlap Graph menggunakan Suffix Tree pada DNA Sequence
Abstrak: The DNA sequence assembly is very important in genome analysis. This step aims to reconstruct contigous DNA fragment (contigs) from short fragment by concatenating fragments based on the overlap region among them. This method employs overlap graph for solving the problems. In this graph, nodes represent fragments and edges represent overlap. This research developed a software for constructing overlap graph using suffix tree. The evaluation was conducted by measuring the number of nodes, number of edges, and the execution time in many values of minimum overlap. The minimum overlap is defined as the minimum length of substring which is considered as the overlap region between two fragments. The evaluation results showed that the number of nodes, the number of edges, and the execution time were increased with the decreasing of the minimum overlap value.
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