Judul: Kualitas Air Cengkareng Drain, Pantai Indah Kapuk, Jakarta Utara Dan Kaitannya Dengan Pasang Surut
Abstrak: Cengkareng Drain merupakan salah satu wilayah yang masih dipengaruhi oleh pasang surut. Perairan Cengkareng Drain dijadikan sebagai titik pengamatan untuk mengkaji keterkaitan antara karakteristik kualitas air dengan pasang surut. Penelitian ini dilakukan satu bulan sekali selama empat bulan pemantauan (Juli-Oktober 2015). Pengambilan contoh air dilakukan pada saat pasang dan surut tiap bulan pemantauan. Indeks Pencemaran menunjukkan bahwa tingkat pencemaran di Cengkareng Drain tergolong tercemar sedang, dengan skor antara 7,7-8,6. Berdasarkan hasil uji t dan analisis similaritas, karakteristik kualitas air pada saat pasang tidak berbeda nyata dengan karakteristik kualitas air pada saat surut. Selama penelitian, nilai total tertinggi pada matrik pembobotan menunjukkan bahwa waktu terbaik pengambilan bahan baku air minum adalah saat surut pada bulan ke-2 pemantauan (Agustus).
Keyword: Cengkareng Drain, kualitas air, pasang surut

Judul: Potensi pemanfaatan air Cengkareng Drain, Pantai Indah Kapuk, Jakarta Utara, sebagai sumber air baku
Abstrak: ADI dan MAJARIANA KRISANTI. Cengkareng Drain merupakan sebuah kanal buatan yang diupayakan dapat dijadikan sebagai sumber air bersih oleh pengelola perumahan Pantai Indah Kapuk. Penelitian ini bertujuan untuk mengevaluasi kesesuaian kualitas air Cengkareng Drain untuk dijadikan sumber air bersih. Penelitian dilaksanakan pada tanggal 28 November-1 Desember 2014. Pengamatan dilakukan setiap satu jam selama 72 jam untuk parameter (DO, pH, temperatur, DHL, TDS, dan salinitas) terkait pasang surut dan satu kali pengamatan dalam sehari pada tiga kedalaman. Analisis data yang digunakan yaitu Indeks Pencemaran (IP) untuk menganalisis kualitas air yang dianalisis di laboratorium dan analisis deskriptif untuk menggambarkan kondisi kualitas air Cengkareng Drain yang diamati secara in situ. Nilai indek pencemaran (IP) pada saat pasang sebesar 8,62, saat surut nilai IP sebesar 5,41. Menurut PPRI No.82 tahun 2001 kelas I perairan tersebut tergolong tercemar sedang disebebkan oleh parameter DO, BOD, COD, total fospat, kekeruhan, raksa, kadmium, selenium, dan total coliform yang tidak sesuai dengan baku mutu perairan. Dengan demikian perairan Cengkareng Drain belum layak untuk dijadikan sumber air baku air minum.
Keyword: Cengkareng Drain, Parameters, Pollution Index, Water Quality

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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