Judul: Arsitektur Internet of Things Berbasis Protokol MQTT di Amazon Web Services
Abstrak: Penelitian ini berfokus pada pembuatan arsitektur Internet of Things (IoT) yang aman dan skalabilitas tinggi. Tujuan dari penelitian ini adalah menghasilkan arsitektur IoT yang dapat diterapkan pada node di lahan pertanian dengan bandwidth rendah . Arsitektur IoT dirancang menggunakan protokol Message Queuing Transport Telemetry (MQTT) yang disediakan oleh Amazon Web Services (AWS). Keluaran dari penelitian ini data yang dikirim dengan protokol MQTT keamanannya terjamin, tidak terjadi packet loss, mempunyai delay sebesar 156 ms tetapi mempunyai nilai overhead yang cukup besar karena payload yang dikirim mempunyai rentang ukuran 90 bytes sampai 150 bytes sedangkan header berukuran 40 bytes.
Keyword: aman, Amazon Web Services, bandwidth rendah, Internet of Things, Message Queuing Transport Telemetry

Judul: Comparative Analysis of MQTT and HTTP Protocol Performance on Air Quality Monitoring IoT Devices.
Abstrak: Kegiatan industri dan transportasi menghasilkan peningkatan konsentrasi materi partikulat yang berbahaya bagi kesehatan masyarakat. Pentingnya memasang alat pemantau udara untuk memantau dan memberikan data yang dibutuhkan untuk mengambil tindakan preventif untuk melindungi kesehatan masyarakat. Dalam upaya menjaga lingkungan, penting untuk membangun alat yang hemat sumber daya. Penelitian ini bertujuan membangun alat pemantau kualitas udara serta analisis konsumsi daya yang digunakan alat. Menggunakan konsep Internet of Things dan protokol komunikasi Message Queuing Telemetry Transport serta Hypertext Transfer Protocol untuk mengirim data, juga menggunakan sensor DSM501A kualitas udara dapat dipantau. Hasil penelitian ini berhasil membangun alat pemantauan kualitas udara menggunakan Internet of Things, mulai dari perancangan alat dan pengiriman data. Selain itu, dari pengujian protokol, QoS 0 memiliki delay dan penggunaan paling rendah baik pada jaringan WiFi dan GSM. Delay sebesar 238 ms dan daya 77,282 mA pada GSM, lalu delay 35 ms dan daya 71,641 mA pada WiFi. Pada semua protokol, semua data yang dikirim tidak menunjukkan adanya packet loss., Industrial and transportation activities result in increased concentrations of particulate matter that are harmful to public health. It is important to install air monitoring devices to monitor and provide data needed to take preventive measures to protect public health. In an effort to protect the environment, it is important to build resource-efficient devices. This research aims to build an air quality monitoring device and analyze the power consumption used by the device. Using the Internet of Things concept as well as Message Queuing Telemetry Transport and Hypertext Transfer Protocol, air quality can be monitored through sensor called DSM501A. The result of this study successfully built an air quality monitoring tool using Internet of Things, starting from tool design and data transmission. In addition, from both protocol testing, QoS 0 has the lowest delay and power usage on both WiFi and GSM networks. Delay of 238 milliseconds and power usage of 77,282 mA on GSM, then delay of 35 milliseconds and power usage of 71,641 mA on WiFi. In all protocols, all data sent showed no packet loss.
Keyword: HTTP, Internet of Things, MQTT, particulate matter, power consumption

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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