Judul: Karakterisasi ekstrak kasar lakase dari isolat jamur liginolitik basidiomesetes Bsd
Abstrak: Lignin merupakan penyusun biomasa tumbuhan yang sulit untuk didegradasi. Salah satu cara untuk mengatasi hal tersebut adalah menggunakan enzim lakase yang dihasilkan oleh jamur Basidimisetes. Penelitian ini bertujuan untuk mengetahui aktivitas dan kondisi optimum reaksi enzim lakase dari isolat jamur Basidiomisetes Bsd menggunakan metode spektrofotomettri.
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Judul: Isolasi, Purifikasi, dan Karakterisasi Enzim Lakase (Lac) dari Jamur Cerrena sp. B.Md.TA.1.
Abstrak: ASEP HIDAYAT. Lakase (EC 1.10.3.2) adalah enzim oksidoreduktase yang menggunakan molekul oksigen untuk mengoksidasi berbagai macam senyawa aromatik. Saat ini lakase banyak dimanfaatkan dalam proses oksidatif, delignifikasi, bioremediasi, dan menjadi subyek penelitian yang menarik sebagai komponen biofuel cell. Penelitian ini bertujuan untuk mengisolasi, memurnikan dan mengkarakterisasi lakase dari Cerrena sp. B.Md.T.A.1 yang diproduksi menggunakan media ampas sagu aren sebagai substratnya. Waktu optimasi produksi fungi terdapat pada hari ke-5.Ekstrak kasar yang diperoleh selanjutnya dimurnikan menggunakan ammonium sulfat dengan kejenuhan 0-20%, 20-40%, 40-60%, 60-80%. Fraksi 40- 60% memiliki aktivitas spesifik tertinggi yaitu sebesar 1742.28 U/mg, kemurnian 2.77 kali, nilai perolehan (yield) 13.497%. Lakase hasil pemurnian fraksi 40-60% kemudian dilakukan desalting menggunakan AKTA Start dan memiliki aktivitas spesifik sebesar 3069.74 U/mg, kemurnian 4.88 kali, nilai perolehan (yield) 1.29%. Lakase hasil desalting mencapai kondisi optimum pada pH 5.0 dan suhu 40°C. Penambahan ion logam Cu2+ dan Mn2+ dengan konsentrasi 1 mM dalam reaksi berperan sebagai aktivator, sedangkan Fe2+ dan NaN3 dengan konsentrasi 1 mM dalam reaksi berperan sebagai inhibitor. Nilai Km dan Vmaks hasil desalting adalah 0.069 mM dan 35.07 U/mL.
Keyword: ampas sagu, Cerrena sp, B.Md.T.A.1, pemurmian lakase

Judul: Penilaian Risiko Kualitatif Pemasukan Virus Avian Influenza (H5N1) ke Pasar Burung Pramuka Jakarta Melalui Unggas
Abstrak: Traditional market or poultry/live birds market was identified as one of avian influenza (AI) virus spot circulation and very potential to contribute on virus spreading to human. Virus infection at the market could not be defined, but the risk value for virus infection can be estimated due to understand well its likelihood and risk factors need to be managed. Qualitative risk assessment had been known as a way to estimate its risk value. Qualitative risk assessment was conducted at Pramuka Birds Market with the purpose to identify biological risk pathways of the entering of Avian Influenza virus (H5N1) and to estimate its risk level through birds. Risk assessment had been done based on the qualitative risk assessment method published by Organization of Internationale Epizootica (OIE) on 2009. The research was conducted on February-June 2012 with primary and secondary data support. Primary data were collected through field observation and questionnaire for 30 sellers and 30 consumers respondents, and through indepth interview with 17 expert respondents, three middlemans and three intstitutions who concern with bird trading. The result of this study identified that there were six pathways of birds supplies to the market defined as risk biological pathways, which are entering of (1) domestic waterfowl, (2) chickens (kampoong, ornamental, or hobbies/pet chickens, (3) wetland birds, (4) “bridges species” birds, (5) songs birds, and (6) exotic birds. Critical control points had been identified in each birds supplies chain are birds collecting fascilities in catcher or collector level, kiosks or sale fascilities, farms, and entry/exit point. Source of birds for the market were (1) nature (capturing from nature), (2) farm, and (3) other country (through importation process). Generally, all groups of birds were important for bringing risk of AI virus entering to PB Pramuka, but both domestic waterfowl and chickens had identified as very import group to bring the risk. Risk estimation value of entering of AI virus (H5N1) to PB Pramuka Jakarta through six groups of poultry/birds originally come from nature, farm and other country was moderate, with the moderate uncertainty. Moderate risk estimation means that before some commodities to be approved for entering the area, it was mandatory to do assessment on risk management implementation, included on its visibility, effectivity, and mechanism of its verification. Based on risk management point of view, moderate risk estimation needed an efforts for risk management implementation to achieve the appropiate level of protection (ALOP) at “negligible” level. This ALOP’s level determined based on characteristic of AI which were zoonotic and had big risk included death both for animal and human. With AI endemic status for Indonesia, still known that AI virus potentially active for mutation, and the findings of high number on entering or selling birds at the market, implementation of applicable risk management must be applied on the birds supplies chain, from the origin until entry the market. Continous surveillance program, development and implementation of operational standard procedures and diseases early warning system, providing appropiate biosecurity fascilities at the market, and better implementation of biosecurity practices with law enforcement support are recommended as risk management actions that
Keyword: assessment, birds, Pramuka Bird Market, qualitative, risk