Judul: Identification of APOA5 Gene Polymorphism and Assosiation With Carcas Characteristic and Lamb Quality in Sheep
Abstrak: Gen apolipoprotein A5 (APOA5) adalah pengatur utama metabolisme trigliserida plasma (TG) yang menunjukkan keterlibatan dalam penyakit arteri koroner. Penelitian ini bertujuan untuk mengidentifikasi gen APOA5 pada karakteristik karkas dan kualitas daging pada domba. Sampel yang digunakan adalah 40 domba yang terdiri dari 10 domba ekor tipis(DET), 10 domba komposit agrinak (DCA), 10 domba garut (DG), dan 10 domba jonggol (DJ). Identifikasi gen APOA5 menggunakan Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Hasil keragaman gen APOA5 bersifat polimorfik pada domba DET, DCA, dan DG, sedangkan DJ bersifat monomorfik. Titik mutasi gen APOA5 berasosiasi (P<0.05) dengan karakteristik karkas, yaitu bobot potong, karkas hangat, persentase karkas, panjang karkas, dan karkas dingin. Selain itu, keragaman gen APOA5 juga berasosiasi signifikan (P<0.05) dengan kualitas daging yaitu pH, keempukan, dan susut masak. Gen APOA5 memiliki kontribusi dalam karakteristik karkas dan kualitas daging pada domba., The apolipoprotein A5 (APOA5) gene is a key regulator of plasma triglyceride (TG) metabolism indicating involvement in coronary artery disease. This study aimed to identify the APOA5 gene polymorphism and their association on carcass characteristics and meat quality in sheep. The samples used were 40 sheep consisting 10 thin tail sheep (DET), 10 compass agrinak sheep (DCA), 10 garut sheep (DG), and 10 jonggol sheep (DJ). Identification of APOA5 gene were performed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The results showed that APOA5 gene diversity were polymorphic in DET, DCA, and DG, while DJ was monomorphic. The mutation the APOA5 gene were significantly associated (P<0.05) with the characteristics of the carcass, carcass weight, warm carcass, carcass percentage, carcass length, and cold carcass. In addition, the diversity of the APOA5 gene was also significantly associated (P<0.05) with lamb quality of the pH, tenderness, and cooking loss. The APOA5 gene has a contribution in carcass characteristics and lamb quality in sheep.
Keyword: APOA5 gene, Carcas characteristic, Lamb quality, PCR-RFLP, Sheep

Judul: Identifikasi Keragaman Gen APOA5 dan Kaitannya terhadap Asam Lemak pada Domba.
Abstrak: Gen apolipoprotein A5 (APOA5) merupakan kunci regulator metabolisme plasma trigliserida plasma (TG) yang menunjukkan keterlibatan dalam penyakit arteri koroner. Penelitian ini bertujuan untuk mengidentifikasi keragaman gen APOA5 dan asosiasinya terhadap asam lemak pada domba. Sampel yang digunakan sebanyak 44 sampel yang terdiri dari 20 ekor domba gemuk (DEG), 16 ekor domba ekor tipis (DET), dan 8 ekor domba komposit garut (DKG). Identifikasi keragaman gen APOA5|BssSI dianalisis menggunakan Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Produk amplifikasi memiliki panjang 258 bp. Hasil identifikasi keragaman gen APOA5 pada semua populasi domba bersifat polimorfik dengan 3 genotipe yaitu CC, CT, dan TT. Hasil chi square menunjukkan gen APOA5 (g.26929941 C>T) berada dalam kesetimbangan Hardy-Weinberg, kecuali pada domba DET. Gen APOA5 pada posisi g.26929941 C>T berasosiasi (P<0.05) dengan asam lemak laurat (C12:0) dan asam lemak EPA (C20:5n3). SNP gen APOA5 dapat digunakan sebagai penanda genetik untuk seleksi domba dengan kandungan asam lemak tak jenuh.
Keyword: asam lemak, domba, gen APOA5, PCR-RFLP

Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4