Judul: Pengaruh Dukungan Sosial dan Strategi Nafkah terhadap Kesejahteraan Subjektif Keluarga Usia Pensiun
Abstrak: This aim of this study was to analyzed the influence of social support and livelihood strategies on subjective well-being at retirement age. This study used cross sectional study design. This research involves 154 families with husband or wives aged greater than or equal to 56 years old and being retired. The samples were selected purposively and grouped into two types (civil servant and non civil servant). Data were collected by interview using questionnaire and analyzed by compare means independent sample t-test and multiple linear regression. Results of this study indicated that subjective well-being civil servant families was higher than the non civil servant. Factors that influence subjective well-being at retirement age family were family income, social support, and livelihood strategies.
Keyword: livelihood strategies, social support, subjective well-being

Judul: Peran Gender dalam Pengambilan Keputusan dan Kesejahteraan Subjektif pada Keluarga Usia Pensiun
Abstrak: This study is aimed to analyze the relationship between gender roles in decision-making with subjective well-being at retirement age family. This study used cross sectional design and involved 154 families with retired husband or wives aged over or 56 years old and selected purposively. Data were collected by interview using questionnaire and was analyzed by descriptive, independent sample t-test, and and Pearson correlation. Education attendance and family’s income were positively significant associated with gender roles in financial management. Number of family dependents were positively significant associated with gender roles in bussiness management. Pearson correlation test results showed that family’s income associated significantly with the economic well-being of the family. The results also demonstrate the role of gender in bussiness management activity is negatively related to the economic subjective well-being of retirement families.
Keyword: gender roles, subjective well-being, Retirement family

Judul: Characterization and Expression of Tomato infectious chlorosis virus Coat Protein Gene in Escherichia coli
Abstrak: Tomato infectious chlorosis virus (TICV) is a member of Crinivirus genus. TICV infects tomato crop in Garut and Cipanas, West Java Indonesia. Tomato initially showed bright interveinal yellowing symptoms. The coat protein gene (CP-TICV) was amplified by RT-PCR from total RNA extracted from infected tomato leaves and the amplified fragment was cloned and completely sequenced. The fragment was subsequently subcloned into the pET-21b expression vector. The recombinant plasmid was transformed to Escherichia coli strain BL21(DE3)pLysS to express the coat protein. The coat protein fused to a 6xhistag, was purified by affinity chromatography using a NiNTA spin column. The identity of the purified protein was confirmed by SDS-PAGE. In this experiment, 792 bp of CP-TICV gene of TICV virus has been successfully cloned, sequenced, and expressed in E. coli. Based on nucleotide sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain strain and 99% identity to North America, France, and California strains and based on amino acid sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain, North America, France strains and 99.2% identity to California strain. CP-TICV showed over expressed in E. coli when it is induced with 1 mM IPTG and incubated at 37oC. Purified CP-TICV-Histag recombinants protein sized 29 kDa based on SDSPAGE analysis.
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