Judul: Multiplikasi Tunas Suweg (Amorphophallus paeoniifolius (Dennst.) Nicolson) dengan Zat Pengatur Tumbuh BAP dan NAA secara Kultur Jaringan
Abstrak: The utilization of suweg (Amorphophallus paeoniifolius) in Indonesia has been known since the Japanese colonial period as source of carbohydrate. Suweg is beneficial for diabetes, digestion, liver, and increase appetite. The cultivation of suweg is by planting its eye shoot of tuber’s skin, the main eye shoot of tuber, the whole tuber, sapling from the tuber, and seedling. But, there are some problems with that technique which are the habitat where it grows, planting time, and the quality of the result is not uniform. So another cultivation effort is needed which is tissue culture. Based on Isnaini et al. (2012), tissue culture of suweg produces few shoots so it needs further study in order to produce many shoots in a callus. This research was done by the addition of Plant Growth Regulators (PGR) NAA and BAP that is potential for shoot growth. The aim of this study is to determine the effect of BAP and NAA in suweg shoot multiplication. This research was conducted at the Laboratory of Plant Tissue Culture Conservation Centre Bogor Botanical Garden, from June until September 2012. The research was designed by using a two-factor factorial experimental design on the basis of completely randomized design (CRD). The first factor is the concentration of BAP with four levels which are 0 mg/l, 1 mg/l, 2 mg/l, and 3 mg/l. The second factor is the NAA concentrations with four levels which are 0 mg/l, 0.25 mg/l, 0.5 mg/l, and 0.75 mg/l. Observed was done to explants browning, contamination (bacteria and fungi), callus color, callus texture, number of shoot, and the number of roots. The result shows the percentage of successful explant survival is 94.17% or 226 explants. Contamination by bacteria and fungi are 2.08 or 5 explants, and browning are 3.75% or 9 explants. Explant with compact texture is 34.58% and explant with crumb texture is 63.33%. The most root growth was found in the treatment of 1 mg /1 BAP + 0.75 mg/l NAA is 2.87 root/ week. The highest number of shoot in the treatment of 1 mg/l BAP + 0.25 mg/l NAA is 0,47 shoot/ week. The conclusion of this study is that the shoot multiplication was occurred in three explants, which are in the treatment of 1 mg l BAP + 0.25 mg/l NAA, 3 m/l BAP + 0.5 mg/l NAA and 3 mg/l BAP + 0.75 mg/l NAA.
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Judul: Pengaruh sitokinin (TDZ) dan auksin (IAA dan NAA) terhadap multiplikasi nenas, Ananas comosus L. Merr. cv. Queen dalam perbanyakan kultur jaringan
Abstrak: The objective of this research was to study the effect of Cytokinin (TDZ) and Auxin (IAA and NAA) as growth regulators in shooting multiplication capacity of shoot base pineapple explant. This research was conducted in tissue culture laboratory at Tropical Fruits Study Center. IPB. Baranangsiang. Bogor. since January until September 2004. Planting material of 5-10 mm base shoot of third subculture Queen pincapple's planlet were cultured in shooting induction medium consisted of MS suplemented with Auxin (IAA and NAA) and TDZ in various concentrations. The experiment was set up in factorial design with two factors, Auxin (5 levels: 0 mg/1. 0.1 mg/l IAA, 0.01 mg/l IAA, 0.1 mg/I NAA and 0.01 mg/l NAA) and TDZ (6 levels: 1x10 mg/l. 1x10 mg/l, 1x10 mg/l, 1x10 mg/l, 1x10 mg/l and 1x10 mg/l). There were 30 combinations treatment. Each treatment was consist of 4 replicates, containing 6 explants per replicate, so that found 720 unit. After 20 weeks in shooting induction medium, explants were transferred to MSO medium for shooting multiplication. Each treatment was consist of 12 replicates. In shooting induction phase, the percentage and the the number of shoot. root. nodule and chap nodule were examined. After subculture to MS free medium, the number of axillar shoot, adventitious shoot and chap nodule were examined every weeks. The weight of nodule were examined in the first and last week of observation. Meanwhile. the number of shoot per gram nodule weight were also examined in the last week of observation. The data were statistically analyzed using completely randomized design and the means were compared with Duncan's Multiple Range Test (DMRT) procedure at 5% level of significance. In the first week of the culture shoot induction medium. explants have shown its growing which marked with crosscut leaves elongation and new leaves formation. In almost treatments, shoot started to be formed at 2 weeks after culture (MST) and rooted at 3 MST. except for the treatments of TDZ at concentration 1x10 mg/l. which is combined with various Auxin concentrations. Explants grown in media supplemented with 1x10 mg/l. 1x105 mg/l, 1x10 mg/l. 1x10 mg/l TDZ in different concentrations of Auxin. initially responded by shoot and root formation with no nodules. There were little number of shoots (with amount of mean less than two) that formed in these culture medium by the end of perception week in both phases, shoot induction phase and shoot multiplication phase. Although the shoots cultured in media supplemented with low concentrations of TDZ (below 1x102 mg/l) were fewer in number. but the shoots were longer and have better quality than those formed in media supplemented with high level concentrations of TDZ (above 1x10 mg/) in the same perception week. Shoots became capable of rooting at combinations of 1x10- 1x10 mg/l TDZ with different concentrations of Auxin, but 1x10 mg/l without Auxin gave rise to the best rooting...dst
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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