Judul: Analisis Harmonik Gelombang Pasang Surut dan Gelombang Permukaan di Teluk Palabuhanratu
Abstrak: Ocean waves such as tides and surface waves affect many activities in marine and coastal area. The purposes of study are to analyze tidal harmonic constituents and wave energy density along with it association with seasonal pattern. Tidal and surface waves measurement held around Sangrawayang, Palabuhanratu using RBR TWR-2050. Recorded data processed utilizing harmonic analysis least square method for tidal wave and distribution and seasonal variability of surface wave. Tidal harmonic analysis show a mixed tides prevailing semidiurnal with Formzahl number is 0.89. Analysis of surface waves shows the wave height ranged between 0.17 - 0.77 meter with dominant period between 10 -15 second categorized as swell. Comparison with Jason-2’s sea surface wave shows there is an association with a seasonal pattern in which the east monsoon wave height trend is higher than the west monsoon.
Keyword: seasonal pattern., harmonic analysis, surface waves, tidal, Palabuhanratu

Judul: A Study On Potential Renewable Energy From Ocean Waves in Palabuhanratu Bay
Abstrak: One of the sources of energy in the sea that can be utilized in Indonesia is wave energy. This study aims to see the potential of ocean wave energy in Palabuhanratu Bay spatially and temporally using a modeling method with the DELFT 3D application. The data sources used are bathymetry data from BIG, wind data from ECMWF, and wave data from Copernicus. Data processing is done by processing bathymetry and wind data first, which is then entered into the model configuration and then adjusted according to the moonson period and scenario. The sea wave power generator (PLTGL) converter scenario is used to estimate the existing potential using a Point Absorber converter. The wind in Palabuhanratu Bay is included in the category of slow gusts with the highest frequency in the category 2,10 – 3,60 m s-1 with a percentage of 30,5%. The sea waves in Palabuhanratu Bay are categorized into Slight Sea with significant wave heights ranging from 0 - 2 m with an average period of 15 seconds. Based on the model results, the wave energy transport has an energy value of up to 0 - 8 kW m-1 with the highest value occurring in the East Season. Based on these results, the Point Absorber type PLTGL produces electric power in Palabuhanratu Bay with two scenarios, the first scenario is in the inner bay which produces 0.09 MW of power with an average energy per day of 261 W. 2,7 MW with an average energy per day of 7344 W.
Keyword: Modelling, Palabuhanratu Bay, Renewable Energy, Waves

Judul: Konstruksi Gen Glukosa Oksidase untuk Subkloning ke dalam Plasmid pPICZα dengan Penambahan Oligonukleotida Penyandi Spacer Peptida
Abstrak: Glukosa oksidase (EC 1.1.3.4) merupakan flavoenzim yang berasal dari Aspergillus niger. Enzim Glukosa Oksidase (GOX) intraseluler yang berasal dari A. niger memiliki aktivitas yang lebih tinggi dibandingkan dengan aktivitas enzim GOX ekstraselulernya. Enzim GOX intraseluler yang berasal dari A. niger memiliki tingkat produksi yang rendah serta proses pemurnian yang cukup rumit. Penelitian ini bertujuan untuk mendapatkan gen penyandi GOX yang siap untuk disisipkan ke dalam plasmid pPICZα untuk kemudian diekspresikan pada Pichia pastoris secara ekstraseluler. Perbanyakan plasmid rekombinan pGEM®-T Easy-GOX dilakukan pada sel bakteri Escherichia coli DH5α. Konstruksi gen GOX dilakukan dengan amplifikasi menggunakan PCR. Amplifikasi dilakukan sebanyak dua kali. Ukuran panjang basa yang diperoleh dari hasil amplifikasi kedua sebesar 1787 bp, terdiri atas situs restriksi XhoI dan oligonukleotida penyandi spacer peptida pada ujung 5’ sekuens gen GOX, serta situs restriksi XbaI pada ujung 3’ sekuens gen GOX.
Keyword: glukosa oksidase, pGEM®-T Easy-GOX, PCR, pPICZα