Judul: Detection of Beta-Amyloid 42 as a Marker of Alzheimer's Disease Using the IPB In-house ELISA Kit
Abstrak: Protein amiloid beta 42 (Aß42) merupakan penanda biologis (biomarker) penyakit Alzheimer yang dapat dideteksi menggunakan kit ELISA. Penelitian ini bertujuan untuk mengetahui performa kit ELISA Aß42 inhouse di level serum dibandingkan dengan kit komersial. Penelitian ini dimaksudkan bermanfaat dalam mendeteksi dini penyakit Alzheimer dan pengembangan penelitian menggunakan kit ELISA inhouse IPB-PSSP. Penelitian ini menggunakan 18 sampel serum manusia yang dibagi menjadi 3 kelompok usia: kelompok 1 (20 – 30 tahun), kelompok 2 (30 – 40 tahun), dan kelompok 3 (> 40 tahun). Nilai OD antar kelompok dan antar kit dibandingkan rata-rata serta standar deviasinya. Hasil pengujian kit ELISA Aß42 inhouse memiliki performa yang mendekati kit ELISA komersial., Amyloid beta 42 (Aß42) protein is a biological marker (biomarker) of Alzheimer's disease that can be detected using an ELISA kit. This study aims to determine the performance of the in-house Aß42 ELISA kit at the serum level compared to the commercial kit. This research is intended to be beneficial for the early detection of Alzheimer's disease and for the development of research using the IPB-PSSP in-house ELISA kit. This study used 18 human serum samples divided into 3 age groups: group 1 (20-30 years), group 2 (30-40 years), and group 3 (> 40 years). The OD values between groups and between kits were compared using the average and standard deviation. The results of testing the in-house Aß42 ELISA kit show performance that is close to the commercial ELISA kit.
Keyword: alzheimer, ELISA

Judul: Reliability Study of Amyloid Kit for Detection of Alzheimer's Disease
Abstrak: Penyakit Alzheimer adalah penyakit neurodegeneratif yang merusak otak secara bertahap dan merupakan bagian dari demensia. Penyakit demensia Alzheimer disebabkan oleh berbagai faktor sehingga sampai saat ini belum diketahui dengan pasti terapi yang tepat. Pembuatan kit sebagai alat diagnostik untuk menunjang pengembangan penapisan terhadap penyakit Alzheimer merupakan upaya strategis yang dapat dilakukan. Penelitian ini bertujuan untuk melakukan kajian reliabilitas kit deteksi amiloid yang dikembangkan menjadi uji alternatif penapisan penyakit Alzheimer dibandingkan dengan kit komersial. Penapisan berbasis pendeteksian dengan teknik Enzyme-Linked Immunosorbent Assay (ELISA) dapat bekerja secara efektif dan akurat dalam mengoptimalkan bahan antibodi spesifik atau antibodi monoklonal terhadap peptida Aβ42. Berdasarkan hasil dan interpretasi dari nilai batas deteksi, koefisien inter dan intra- assay, kit ELISA yang dikembangkan secara in house memiliki kemampuan deteksi Aβ42 yang menyamai Kit ELISA komersial yang telah tersedia (MyBioSource MBS288507). Kata kunci: Aβ42, ELISA, penapisan, penyakit Alzheimer, reliabilitas kit, Alzheimer’s Disease is a progressive neurodegenerative disease that damages the brain and is part of dementia. Alzheimer’s dementia is caused by various factors that until now the proper therapy is uncertain. Kit production as a diagnostic tool to support the development of screening for Alzheimer’s disease is a strategic effort. Screening based on detection with Enzyme-Linked Immunosorbent Assay (ELISA) can work effectively and accurately to optimize a specific antibody material or monoclonal antibody to the Aβ42 peptide. This study aimed to research the reliability of the amyloid detection kit which was developed as an alternative screening test for Alzheimer’s disease compared to commercially available kits. Based on the results and interpretation of limits of detection, inter and intra-assay coefficients, the in-house ELISA kit has comparable Aβ42 detection to the commercially available ELISA kit (MyBioSource MBS288507). Keywords: Aβ42, Alzheimer’s disease, ELISA, reliability kit, screening
Keyword: ELISA, Reliabilitas kit, Macaca fascicularis, Penyakit Alzheimer, Penapisan, Aβ42

Judul: Thermal Characteristics and Dynamics of Tropical Cyclone over Western North Pacific Ocean: A Case Study of Typhoon Songda (Chedeng) 2011
Abstrak: The frequent occasion of Tropical Cyclone (TC) over Western North Pacific (WNP) Ocean lies from 10˚N to 26˚N and 121˚E to 170˚E. Sources of TC development come from warm ocean temperature where latent heat is released during condensation processes and subsequently concentrated in the boundary layer. Relative humidity at 850-700 hPa is responsible to produce the most intense storm. Meanwhile, weak vertical wind shear between 850-200 hPa is one of the dynamical factors that lead to the development of TC. Pressure level of 850-200 hPa is chosen to minimize the thermodynamic effect in the lower troposphere. Typhoon Songda (Chedeng in the Philippines) or TSC lasted from May 18th to 30th, 2011 and strengthened into powerful Super Typhoon stage between May 24th and 27th. Long curving track and the exhibition of all sequences of TC life cycle, TSC is then considered as an ideal case of TC. The objective of the study with case of TSC is to investigate the role of thermal characteristics and dynamics on TC intensity. The research is carried out by using Advanced Research of Weather Research and Forecasting model (WRF ARW model) version 3.3. Two domains are employed in the model. The first domain situate from 02˚N to 40˚N and 118˚E to 149˚E and cover spatial resolution () of 33.1 km. The second domain situate from 09˚N to 27˚N and 119˚E to 132˚E with of 11 km. Timestep of the model () is 120 seconds. The model is supported by the following physical parameterization, i.e., Yonsei University Scheme (YSU) in the boundary layer, Kain-Fritsch scheme (KF) in the cumulus, and WRF Single-Moment 3-class scheme in the microphysics. Initial meteorological condition prior to the formation of the cyclone is generated using WRF ARW standard-release model. Sensitivity of the model output is obtained from a selected single-parameter change scenarios, namely, 10 % to 20 % decrease and increase of wind shear initial data at 850-200 hPa, and by 10 % to 20 % decrease of relative humidity initial data at 850-700 hPa. The result showed that the negative correlations between wind shear and intensity are found in wind shear scenarios. Wind shears of 10 ms-1 which lead to strengthening of the wind intensity in the early period of Typhoon development are found in wind shear scenarios. The effect of wind shear changes on wind intensity is statistically significant in the 20 % wind shear scenarios. During mature stage, weak wind intensity occurred when wind shear increase as given in the WRF standard-release and the 20 % decreasing scenario. Different situation is found in the 20 % increasing scenario where wind shear tend to weak and lead to strong wind intensity at this stage. Meanwhile, the reduction of moisture supply at 850-700 hPa resulting from 20 % decreasing relative humidity play a major role in the weakening of wind intensity and reduces mechanical energy of the cycle process as much as 300 J kg-1.
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