Judul: Fermentasi Kedelai Hitam Detam 2 oleh Bacillus subtilis natto untuk Meningkatkan Kandungan Isoflavon Aglikon
Abstrak: Fermentation is a process that most humans do to improve value of nature material. Bacillus subtilis natto (B.natto) fermentation was expected to increase the content of aglycone isoflavones that beneficial for health. The aim of this study was to measure the amount of isoflavone aglycone daidzein and genistein in fermented black soybeans varieties detam 2 with B.natto strain IFO 3335. HPLC analysis of aglycone showed defatted and undefatted of raw soybeans sample for 100 g dry weight are 1.29 mg and 1.19 mg for genistein and 1.16 mg and 1.07 for daidzein. After fermentation both genistein dan daidzein concentration increased up to 8 times higher than raw soybeans. Genistein concentration increase until 10.43 mg for defatted and 9.43 mg undefatted sample and daidzein concentration is 9.60 mg for deffated and 8.68 mg for undefatted sample. This values were higher than non-fermented soy that only contain genistein and daidzein, respectively 1.07 mg and 1.09 mg for defatted sample and also 0.97 mg and 0.98 mg for undefatted sample. Fermentation with B.natto was proven to improve the consentration of genistein and daidzein in black soybeans varieties detam 2.
Keyword: soybean, genistein, fermentation, daidzein

Judul: Genistein and Daidzein Content of Soybean, Tofu Waste, and “Oncom Merah”.
Abstrak: Isoflavone (genistein and daidzein) can be found in Leguminosae, especially in soybean. Soybean can be processed into tofu and get around to tofu waste, which can be processed finally to “oncom merah”. Soybean, tofu waste, and “oncom merah” had been analysed in this research for genistein and daidzein contents. Soybean, tofu waste, and “oncom merah” were hydrolysed using 4 N HCl and ethanol, followed by partition using ethyl acetate. Components purification by flash chromatography and its contents were determined by high performance liquid chromatography. Genistein and daidzein contents of soybean were 128.69 mg/100g and 134.46 mg/100g, respectively. Tofu waste contained 65.93 mg/100g genistein and 63.68 mg/100g daidzein. Furthermore, genistein and daidzein contents of “oncom merah” were 32.41 mg/100g and 27.70 mg/100g. Soybean contained genistein and daidzein two times higher than tofu waste and four times higher than “oncom merah”.
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Judul: Penyelesaian Stacker Crane Problem dengan Algoritme Largearcs dan Smallarcs
Abstrak: In graph theory, the Rural Postman Problem (RPP) aims to determine the shortest route with minimum cost, in which only certain edges or arcs are necessarily traversed. One type of RPP is Stacker Crane Problem (SCP) which deals with a mixed graph but only arcs are necessarily traversed. SCP can be solved by using two heuristic algorithms, i.e. Largearcs and Smallarcs algorithms, which include other algorithms in their steps, such as: Dijkstra algorithm to determine shortest route, Hungaria method to determine minimum bipartite matching, Prim algorithm to determine minimum spanning tree, and van Aardenne-Ehrenfest & de-Bruijn algorithm and Fleury algorithm to determine Euler circuits. The shortest Euler circuit resulted from two heuristic algorithms can be used to find a solution of SCP. The application of SCP is illustrated in establishing the minimum distance route of catering delivery.
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