Judul: Sterilization and Induction of Kluwih Teak Callus (Tectona abludens var. lacerata) In Vitro Culture
Abstrak: Tectona abludens merupakan tanaman endemik Jawa khususnya Gunung Kidul dan Bantul DIY yang hanya ditemukan 11 individu pohon. Perkembangbiakan T. abludens memiliki permasalahan karena diduga memiliki hambatan fisik maupun fisiologis, sehingga diperlukan upaya perbanyakan dengan teknik in vitro. Permasalahan utama dalam propagasi in vitro jati kluwih adalah sterilisasi eksplan dan induksi kalus. Penelitian ini bertujuan menganalisis respon waktu perendaman merkuri klorida (HgCl2) terhadap sterilisasi eksplan dan menganalisis respon kombinasi Zat Pengatur Tumbuh (ZPT) 2,4-D dan kinetin terhadap pembentukan kalus embriogenik. Metode analisis data yang digunakan adalah Rancangan Acak Lengkap (RAL) untuk sterilisasi eksplan dan Rancangan Acak Lengkap Faktorial (RALF) untuk induksi kalus. Hasil penelitian sterilisasi ekplan menunjukkan perlakuan perendaman 300 mg/l HgCl2 selama 15 menit menghasilkan eksplan steril tertinggi 90% pada 15 HST. Hasil penelitian induksi kalus menunjukkan perlakuan 0 ppm 2,4-D yang dikombinasikan 1,5 ppm kinetin menghasilkan persentase kalus tertinggi 73,33% pada 12 MST., Tectona abludens is an endemic plant of Java, especially Gunung Kidul and Bantul DIYwhich only found 11 individual trees. The breeding of T. abludens has problems because it is thought to have physical and physiological obstacles, so propagation efforts are needed with in vitro techniques. The main problems with in vitro techniques are explant sterilization and callus induction. This study aims to analyze the response of mercury chloride (HgCl2) immersion time to explant sterilization and analyze the response of a combination of 2,4-D Plant Growth Regulators (PGR) and kinetin to embryogenic callus formation. The data analysis methods used were Complete Randomized Design (CRD) for explant sterilization and Factorial Complete Random Design (FCRD) for callus induction. The results of the explant sterilization study showed that soaking treatment of 300 mg /l HgCl2 for 15 minutes resulted in the highest sterile explant of 90% at 15 HST. The results of callus induction research showed that 0 ppm 2,4-D treatment combined with 1.5 ppm kinetin resulted in the highest callus percentage of 73.33% at 12 MST.
Keyword: callus induction, in vitro, sterilization, Tectona abludens

Judul: Induction and Regeneration of Callus Garlic (Allium Sativum L.) Lumbu Kuning Cultivar in Several Media Compositions
Abstrak: Penelitian ini bertujuan mempelajari pengaruh beberapa kombinasi zat pengatur tumbuh pada beberapa media untuk mendapatkan kalus embriogenik bawang putih kultivar Lumbu Kuning secara in vitro. Percobaan menggunakan Rancangan Acak Lengkap satu faktor yaitu komposisi media. Terdapat 4 taraf komposisi media yaitu media A (0,1 ppm 2,4-D + 0,5 ppm Kinetin), media B (0,3 ppm 2,4-D), media C (1 ppm 2,4-D + 2 ppm Kinetin + 2 ppm IAA) dan media D (0,3 ppm 2,4-D + 0,5 ppm Kinetin) yang dikelompokkan dalam 10 kelompok sebagai ulangan sehingga terdapat 40 satuan percobaan. Pada subkultur 2 dilakukan pemindahan kalus ke media dengan mengurangi konsentrasi ZPT setengah dari awalnya di setiap media. Pada subkultur ke 3 semua kalus dipindah ke media dengan BAP 2 ppm + NAA 0,1 ppm. Hasil penelitian menunjukkan bahwa seluruh media perlakuan hampir bersamaan meginduksi kalus, dan dapat menginduksi kalus 100%. Media A, pada subkultur 3 menghasilkan ukuran kalus lebih besar dibandingkan tiga media lainnya. Kalus remah paling banyak muncul pada media B dan media C pada subkultur ketiga dengan persentase 100%. Kalus beregenerasi membentuk akar (7,5%), embrio somatik fase globular, heart, scutellum dan koleoptil, serta tunas (2,5%)., This research aimed to study the effect of media compositions to obtain garlic embryogenic callus of Lumbu Kuning cultivar. The experiment is designed using a completely randomized design with a single factor, that was the composition of the media. There are 4 levels of media composition, media A (0,1 ppm 2,4-D + 0,5 ppm Kinetin), media B (0,3 ppm 2,4-D), media C (1 ppm 2,4-D + 2 ppm Kinetin + 2 ppm IAA), and media D (0,3 ppm 2,4-D + 0,5 ppm Kinetin) were grouped into 10 groups as replications so that there were 40 experimental units.In subculture 2, callus was transferred to the media by reducing the ZPT concentration by half from the beginning in each media. in the 3rd subculture, all callus was transferred to the media with BAP 2 ppm + NAA 0,1 ppm. The results showed that all treatment media induced callus almost simultaneously, and could induce callus 100%. Media A, in subculture 3 produced a largest callus size than the other three media. Crumb callus mostly appeared on media B and media C in the third subculture with a percentage of 100%. Callus regenerated to form roots (7,5%), somatic embryos in globular phase, heart, scutellum and coleoptile, also shoots (2,5%).
Keyword: 2,4-D, BAP, lumbu kuning, basal plate, somatic embryogenesis

Judul: Approximation of the Median of Poisson Distribution Using the Relation of Poisson-Gamma Distribution
Abstrak: A major drawback of median is that median cannot usually be defined in closed form, even in the cases where their distribution functions are known. This study gives an approximation of the closed form for the median of the Poisson distribution by using the relation of Poisson-Gamma distribution. The approximation uses elementary techniques based on the monotonicity of certain sequences involving tail probabilities of the Poisson distribution and the Central Limit Theorem. Furthermore, a closed form expression for the mean absolute deviation 􀜧|􀜰􀰒 􀵆 􀜯􀝁􀝀􁈺􀜰􀰒􁈻| is also studied. The median is relevant because of the fact that it minimizes the mean absolute deviation of a Poisson random variable (􀜰􀰒􁈻 by considering median as central tendency.
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