Judul: Rezim Air Tanah dan Emisi Gas Rumah Kaca di Hutan Rawa Gambut PT. Rimba Makmur Utama, Katingan, Kalimantan Tengah
Abstrak: Peat swamp forests provide ecosystem services such as hydrological system balancing for the surrounding area which is characterized by its ability to maintain water level. Measurements of soil water rezime is important to understand its response of equilibrium greenhouse gases flux in peatland. In this research, water level measurements performed using HOBO U20 placed in the 4 observation wells. In addition, the CO2, N2O and CH4 flux measurements by the closed chamber method. Soil water rezime was affected by rainfall and peat soil characteristics including land cover types. Results showed that there was delay in peat to response the rainfall for about one month in the rainfall monthly analysis. Water level was negatively correlated with the CO2 emissions into the atmosphere, 0.75±0.06 gr/m2/hr (bush) and 0.46±0.02 gr/m2/hr (secondary forest) in dry condition. Methane (CH4) fluxes were higher in waterlogger peat condition reached 24.57±5.95 mg/m2/hr (bush) and 17.10±3.76 mg/m2/hr (secondary forest), while N2O did not show a significant affected by soil water rezimes.
Keyword: Bogor Agricultural University (IPB), water level., bush, secondary forest, Gas flux

Judul: Emisi Gas Co2, Ch4 Dan N2o Pada Ekosistem Hutan Rawa Gambut: Studi Kasus Di Kabupaten Katingan, Kalimantan Tengah
Abstrak: Perbedaan tutupan lahan dapat menjelaskan variasi fluks CO2, CH4 dan N2O yang dihasilkan dari lahan gambut. Penelitian ini dilaksanakan untuk mengukur dan mengkaji emisi pada hutan sekunder dan semak di hutan gambut PT Rimba Makmur Utama Katingan, Kalimantan Tengah pada bulan Mei dan Agustus 2014. Fluks CO2 diukur dengan EGM-4 serta inkubasi dengan perlakuanWFPS, sedangkan CH4 dan N2O dianalisis melalui khromatografi gas dengan metode sungkup tertutup. Pada bulan Agustus, fluks CO2 pada tutupan lahan semak (53,52 Mg/ha/tahun) lebih tinggi dibanding emisi pada hutan rawa sekunder (51,53 Mg/ha/tahun) seiring peningkatan suhu dan penurunan tinggi muka air. Fluks CH4 tertinggi dicapai pada kondisi tergenang di tutupan lahan semak (2,10 Mg/Ha/tahun) dan menurun pada kondisi kering di hutan rawa sekunder (0,08 Mg/Ha/tahun). Fluks N2O pada tutupan lahan semak mencapai 0,65 Mg/ha/tahun pada musim hujan, dan menurun pada musim kemarau hingga -0,16 kg/Ha/tahun yang menunjukkan mekanisme serapan N2O. Hasil inkubasi menunjukkan fluks CO2 maksimum pada WFPS 40% pada contoh tanah dari hutan rawa sekunder dan WFPS 100% dari tutupan lahan semak.
Keyword: fluks, hutan rawa sekunder, inkubasi, khromatografi gas, semak

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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