Judul: Studi Pustaka Pengaruh Aplikasi Probiotik untuk Pencegahan Penyakit Motile Aeromonas Septicemia pada Ikan Lele Clarias sp.
Abstrak: Penggunaan probiotik sebagai alternatif dari antibiotik untuk penyakit bakterial Motile Aeromonas Septicemia (MAS) yang disebabkan oleh bakteri Aeromonas hydrophila. Mekanisme kerja probiotik yaitu melekat pada mukosa usus, menghasilkan zat antibakteri, mengoptimalkan produksi enzim pencernaan, memelihara populasi mikroba di dalam usus, serta meningkatkan pemanfaatan pakan, pertumbuhan dan sintasan. Penelitian bertujuan mengumpulkan, merangkum dan mengkaji berbagai pustaka mengenai aplikasi probiotik dengan jenis dan dosis yang berbeda untuk mencegah penyakit Motile Aeromonas Septicemia (MAS) pada ikan lele (Clarias sp.). Penelitian dilakukan di Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor pada bulan Juli 2023 sampai Januari 2024 dengan metode studi literatur. Probiotik dapat mengubah mikrobiota usus, meningkatkan ketahanan terhadap penyakit, meningkatkan kelangsungan hidup, dan menjaga kualitas air selain memperkuat dan meningkatkan respon imun. Pemberian probiotik jika dibandingkan dengan perlakukan kontrol (tanpa probiotik) mampu meningkatkan respon imun humoral dan seluler serta imunitas ikan lele terhadap patogen Aeromonas hydrophila penyebab penyakit Motile Aeromonas Septicemia. Dosis probiotik untuk ikan lele sebaiknya berkisar antara 105 hingga 107 CFU mL-1 untuk memaksimalkan respon imunologi dan efek kelangsungan hidup sebesar 60–90%., The use of probiotics as an alternative to antibiotics for the bacterial disease Motile Aeromonas Septicemia (MAS) which is caused by the bacteria Aeromonas hydrophila. The mechanism of action of probiotics is that they attach to the intestinal mucosa, produce antibacterial substances, optimize the production of digestive enzymes, maintain the microbial population in the intestine, and increase feed utilization, growth and survival. The research aims to collect, summarize and review various literature regarding the application of probiotics with different types and doses to prevent Motile Aeromonas Septicemia (MAS) disease in catfish (Clarias sp.). The research was conducted at the Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural Institute from July 2023 to January 2024 using the literature study method. Probiotics can alter gut microbiota, increase disease resistance, improve survival, and maintain water quality in addition to strengthening and improving the immune response. Probiotic administration compared with the control treatment (without probiotics) was able to increase the humoral and cellular immune response as well as the immunity of catfish against the pathogen Aeromonas hydrophila which causes the disease Motile Aeromonas Septicemia. The probiotic dose for catfish should be in the range of 105 to 107 CFU mL-1 to maximize the immunological response and survival effect of 60–90%.
Keyword: Aeromonas hydrophila, catfish, probiotic, MAS

Judul: The Application of Probiotic Staphylococcus lentus L1k on Production Performance and Immune Response of Catfish (Clarias sp.) Infected with Aeromonas hydrophila
Abstrak: Produksi ikan lele yang terus meningkat memerlukan pengembangan budidaya dengan sistem intensif, namun penyebaran penyakit Motile Aeromonas Septicemia yang disebabkan oleh bakteri Aeromonas hydrophila dalam budidaya intensif masih menjadi masalah. Pencegahan penyakitnya dapat dilakukan dengan meningkatkan respons imun ikan melalui pemberian probiotik. Penelitian ini bertujuan menguji pengaruh aplikasi probiotik Staphylococcus lentus L1k dengan dosis yang berbeda terhadap kinerja produksi dan respons imun ikan lele (Clarias sp.) yang diinfeksi Aeromonas hydrophila pada dosis yang efektif. Penelitian terdiri atas lima perlakuan yaitu K+ (tanpa pemberian probiotik dan diuji tantang), K- (tanpa pemberian probiotik dan tanpa uji tantang), A (L1k 104 CFU mL-1 1% dan diuji tantang) B (L1k 106 CFU mL-1 1% dan diuji tantang), dan C (L1k 108 CFU mL-1 1% dan diuji tantang). Ikan uji memiliki bobot awal (29,49 ± 0,04) g dipelihara selama 30 hari dan diberi pakan suplementasi, kemudian diuji tantang dengan bakteri A. hydrophila selama 10 hari. Hasil penelitian menunjukkan pemberian probiotik Staphylococcus lentus L1k mampu meningkatkan kinerja produksi dan respons imun ikan lele (Clarias sp.) yang diinfeksi bakteri Aeromonas hydrophila dengan dosis terbaik pada pakan dengan penambahan bakteri Staphylococcus lentus L1k dengan dosis 104 CFU mL-1., The continuously increasing production of catfish requires the development of intensive aquaculture systems. However, the spread of Motile Aeromonas Septicemia disease caused by the bacterium Aeromonas hydrophila in intensive aquaculture remains a problem. Disease prevention can be achieved by enhancing the fish's immune response through the administration of probiotics. This study aims to test the effect of the application of Staphylococcus lentus L1k probiotics at different doses on the production performance and immune response of catfish (Clarias sp.) infected with Aeromonas hydrophila at an effective dose. The study consists of five treatments: K+ (no probiotic administration and challenged), K- (no probiotic administration and not challenged), A (L1k 104 CFU mL-1 1% and challenged), B (L1k 106 CFU mL-1 1% and challenged), and C (L1k 108 CFU mL-1 1% and challenged). The test fish had an initial weight of (29.49 ± 0.04) g and were reared for 30 days with supplemental feed, then challenged with A. hydrophila bacteria for 10 days. The results of the study show that the administration of Staphylococcus lentus L1k probiotics can improve the production performance and immune response of catfish (Clarias sp.) infected with Aeromonas hydrophila, with the best dose being 104 CFU mL-1 when added to the feed.
Keyword: Aeromonas hydrophila, catfish, Motile Aeromonas Septicemia, Staphylococcus lentus L1k

Judul: Penyusunan Overlap Graph menggunakan Suffix Tree pada DNA Sequence
Abstrak: The DNA sequence assembly is very important in genome analysis. This step aims to reconstruct contigous DNA fragment (contigs) from short fragment by concatenating fragments based on the overlap region among them. This method employs overlap graph for solving the problems. In this graph, nodes represent fragments and edges represent overlap. This research developed a software for constructing overlap graph using suffix tree. The evaluation was conducted by measuring the number of nodes, number of edges, and the execution time in many values of minimum overlap. The minimum overlap is defined as the minimum length of substring which is considered as the overlap region between two fragments. The evaluation results showed that the number of nodes, the number of edges, and the execution time were increased with the decreasing of the minimum overlap value.
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