Judul: Analisis keragaman D-Loop Dna mitokondria mencit rumah (Mus Musculus Castaneus) di daerah Jakarta, Bandung dan Surabaya dengan Pcr-Rflp
Abstrak: House mice ( Mus musculus) belongs to class Mammal, infraclass Eutheria, Order Rodentia, suborder Myomorpha and family Muridae. This spesies divided into subspesies subspesies Mus musculus domesticus, Mus musculus musculus, Mus musculus castaneus and Mus musculus bactrianus (Silver 1995). Subspesies which spread all over Indonesia is Mus musculus castaneus. Mitochondrial DNA (mtDNA) is often used as genetic marker to study genetic diversity. Genetic diversity of mtDNA can be obtained using PCR-RFLP. The Primers used for analysis were CTDF and CTDR. The restriction enzyme used were HaeIII, AluI and RsaI. The samples were from Jakarta (10 mice), Bandung (11 mice) and Surabaya (9 mice) so the total amount of samples were 30 mice. We generated pcr product 1384 bp in length using PCR-RFLP. Nine haplotype were found. In Jakarta population there were three haplotype, Bandung seven haplotype and Surabaya five haplotype. Local haplotypes were found in Bandung (H-5 and H-7) and Surabaya (H-8 and H-9). The H-3 was common haplotype that were found in all population.
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Judul: Analisis keragaman genetik labi-labi, dogania Subpkma (Testudines: trionychidae), melalui teknik PCR-RFLP
Abstrak: Saat ini telah terjadi penurunan populasi labi-labi yang sebagian besar diakibatkan adanya perburuan liar dan kerusakan lingkungan. Melihat hal itu diperlukan suatu langkah untuk mengumpulkan berbagai informasi biologi labi-labi. Salah satu caranya adalah dengan melakukan anal isis molekular sebelum berbagai informasi lainnya diungkapkan. Penanda molekular genetik yang sering digunakan dalam analisis molekular adalah daerah pengendali (d-loop) genom mitokondria menggunakan teknik PCR-RFLP. Bagian yang paling stabil dari daerah pengendali mtDNA diamplifikasi secara in vitro dengan pasangan primer 577 dan 578. Produk PCR dipotong dengan lima macam enzim restriksi, yaitu Alu I, Hsp92 II, Hha I, Hpa II dan Rsa I yang menghasilkan tujuh haplotipe. Keragaman nukleotida antara haplotipe yang ditemukan di Jawa dan Sumatera (interlokasi) adalah 12.11 %. Angka itu jauh lebih besar dibanding keragaman nukleotida antar haplotipe di dalam Sumatera maupun Jawa (intralokasi) yang berturut-turut adalah 6.5% dan 3%.
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Judul: Pembuatan Film Tipis Cu2ZnSnS4 sebagai Lapisan Penyerap Cahaya pada Sel Surya Menggunakan Metode Elektrodeposisi
Abstrak: Thin film of Cu2ZnSnS4 (CZTS) is an alternative semiconductor used as light absorber layer on solar cell. CZTS can replace silicon that commonly used as commercial semiconductor. The advantage of using CZTS as absorber layer is due to the abundance of its chemical composer on earth, and its high value of absorption coefficient. The absorber layer was made by using electrodeposition method on ITO glass substrates. The films were deposited at room temperature using potentiostatic mode with a potential of –1.05 V for 30, 45, and 60 minutes. After that, the films were annealed at two different temperatures, 180 ºC and 500 °C, for 45 minutes in N2/H2S atmosphere. Based on X-ray diffraction analysis, the polycrystalline thin film obtained fall into kesterite crystal phase. This type of crystal was obtained when the CZTS was annealed at 500 °C in H2S/N2 atmosphere. The value of optical band gap energy for the CZTS thin film was 2.2 eV with the film thickness about 1.228–6.366 μm. The sample of CZTS with electrodeposition time of 45 minutes and annealing temperature of 500 °C showed the best result and in conformity with absorber layers criteria for solar cells.
Keyword: semiconductor, ITO glass, CZTS thin film, electrodeposition