Judul: Manajemen Sumber Daya Keluarga dan Ketahanan Keluarga Lanjut Usia
Abstrak: This study is conducted to analyze the influence of family resource management (FRM) to family strength on aging families. The data was collected on June-July 2012 purposively at three villages (Ciherang, Cikarawang, and Babakan) which are included to five areas with the largest aging family populations in Bogor. Interviews were conducted on 34 early-elders (60-75 years old) who still have spouses and have no dependents. FRM was measured with human resource management, time management, and financial management of all phases of the family. Family strength was measured with physical strength, psychological strength, and social strength. The result showed that average of family strength aging families had performance by three-quarters of family strength ability (75% for physical strength, 68% for psychological strength, dan 83% for social strength). Performance index of FRM ability had only achieve half of FRM indicators (81% for human resource management, 19% for time management, and 71% for financial management). FRM had influence on family strength. Length of education and of marriage and percapita income had a significant positive influence on family strength.
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Judul: Pengaruh Kerentanan, Perubahan, dan Stabilisasi Berdasarkan Siklus Perkembangan Keluarga Terhadap Kualitas Hidup Lanjut Usia
Abstrak: The research aimed to analyze the effect of family characteristics, vulnerability, family life change, and family stabilization toward the elderly‟s quality of life. This research design is retrospectif and cross sectional. Samples of this research were 34 elderly (60-75 years), who still a spouse, haven‟t had, and used purposively selected. The study was conducted in Dramaga subdistrict and used interviews with questionnaires. Quality of life is divided into two that are subjective and objective. Subjective quality of life is measured by perceptions of physical health, social relationship, and environmental. The objective quality of life measured by income, health, education and basic needs. The results showed more than half of the total sample had a relatively high quality of life. Stabilization of the family, family life changes, and vulnerability was low. Quality of life influenced age, length of married, and number of children.
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Judul: Characterization and Expression of Tomato infectious chlorosis virus Coat Protein Gene in Escherichia coli
Abstrak: Tomato infectious chlorosis virus (TICV) is a member of Crinivirus genus. TICV infects tomato crop in Garut and Cipanas, West Java Indonesia. Tomato initially showed bright interveinal yellowing symptoms. The coat protein gene (CP-TICV) was amplified by RT-PCR from total RNA extracted from infected tomato leaves and the amplified fragment was cloned and completely sequenced. The fragment was subsequently subcloned into the pET-21b expression vector. The recombinant plasmid was transformed to Escherichia coli strain BL21(DE3)pLysS to express the coat protein. The coat protein fused to a 6xhistag, was purified by affinity chromatography using a NiNTA spin column. The identity of the purified protein was confirmed by SDS-PAGE. In this experiment, 792 bp of CP-TICV gene of TICV virus has been successfully cloned, sequenced, and expressed in E. coli. Based on nucleotide sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain strain and 99% identity to North America, France, and California strains and based on amino acid sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain, North America, France strains and 99.2% identity to California strain. CP-TICV showed over expressed in E. coli when it is induced with 1 mM IPTG and incubated at 37oC. Purified CP-TICV-Histag recombinants protein sized 29 kDa based on SDSPAGE analysis.
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