Judul: Analisis kanonik hubunga indikator kependudukan dengan derajat kesehatan
Abstrak: Bernat Simbolon. Analisis Kanonik Hubungan Indikator Kependudukan dengan Derajat Keseliaran ( di bawah bimbingan Bambang Sumantri dan Abdurrauf Rambe). Penelitian ini berujuan utrtuk menggambarkan tingkat kekuatan hubungan beberapa indikator kependudukan terhadap derajat kesehatan dan mengelompokkan propinsi di Indoriesia berdasarkan kumpulan peubah tersebut. Fakror-fakror yang mampu memberi irformasi dasar mengenai indikator kependudukan adalah kesehatan, gizi, pendidikan, ckononri, sosial budaya dun lingkungan sosial. Sedangkan derajat kesehatan secara kuantitatifdiukur dari angka kematian bayi, angka kematian balita dan harapan hidup masyarakat (laki-laki dan wanita).
Keyword: 

Judul: Penentuan Indeks Kualitas Gizi Dan Kesehatan Penduduk Indonesia
Abstrak: Untuk mengetahui perkembangan kualitas gizi dan kesehatan penduduk, dan dapat pula dibandingkan keragaan antar wilayah, bahkan antar negara, diperlukan suatu indeks. Tujuan penelitian ini adalah untuk menentukan Indeks Kualitas Gizi dan Kesehatan Penduduk Indonesia sebagai salah satu bagian dari indeks kualitas hidup. khusus penelitian ini bertujuan untuk : Secara 1) Memilih metode penyusunan Indeks Kualitas Gizi dan Kesehatan Penduduk di tingkat propinsi yang dapat diandalkan melalui pembanding- an Metode Observasi, Metode Normatif dan Metode Sebaran, 2) Mengetahui hubungan Indeks Kualitas Gizi dan Kesehatan Penduduk dengan Tingkat Pendidikan, 3) Mengetahui hubungan Indeks Kualitas Gizi dan Kesehatan Penduduk dengan Tingkat Pendapatan. Penelitian ini menggunakan data gizi dan kesehatan yang tersedia di Biro Pusat Statistik dan Departemen Kesehatan RI. Data tersebut merupakan hasil SUSENAS 1987, 1990, 1992, 1993; SUPAS 1985; SP 1990; dan SKRT 1992. Peubah-peubah yang digunakan dalam penelitian ini adalah peubah yang teridentifikasi pada pemilihan contoh, terse- dia di tingkat pusat secara periodik, memiliki akurasi yang baik, serta mudah dan murah dikumpulkan. Sifat indeks yang diinginkan adalah : a) Peubah- peubah yang menjadi komponen indeks adalah peubah-peubah penting dan merupakan indikator output pembangunan sumber- daya manusia, b) Dengan semakin tinggi indeks,….dst
Keyword: Kualitas Gizi, Kesehatan Penduduk, Indeks Mutu Hidup, Pembangunan Manusia, Pembangunan Pangan, Gizi dan Kesehatan

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable