Judul: Weather Element Observation using Wireless Sensor Networks
Abstrak: Penelitian ini bertujuan untuk mengembangkan sistem Wireless Sensor Networks (WSN) dengan menggunakan mikrokontroler ESP32, LoRa, dan sensor BME280 sebagai salah satu pilihan alternatif observasi di dunia meteorologi dengan biaya yang relatif murah serta membantu meningkatkan resolusi spasial pengamatan unsur cuaca. Metode dalam penelitian ini adalah membangun sistem menggunakan sensor BME280 yang dihubungkan ke ESP32, dan LoRa sebagai metode komunikasi. Sistem dilengkapi dengan website, aplikasi android, dan panel kendali berbasis Command Line Interface (CLI). Website menyediakan tampilan grafik dari pengamatan real-time. Aplikasi android digunakan untuk tag lokasi, tracking lokasi untuk pengamatan bergerak, serta melihat data pengamatan real- time. Panel kendali berfungsi mengendalikan sistem dari jarak jauh menggunakan internet. Sistem bekerja dengan cara membaca hasil sensor lalu dikirimkan ke node- node terdekat menggunakan metode komunikasi LoRa sampai ke base station, lalu dikirim ke database menggunakan internet. Pengujian sistem WSN ialah untuk mengukur parameter cuaca yaitu suhu, kelembaban relatif, dan tekanan udara. Pengamatan parameter cuaca hanya sebagai contoh kecil penggunaan sistem WSN, karena penggunaannya bisa untuk bebagai unsur lingkungan. Hasil pengujian menunjukkan bahwa sistem WSN berfungsi dengan baik dan dapat memantau perkembangan kondisi cuaca di berbagai titik pengamatan. Konfigurasi node harus diperhatikan untuk kelancaran komunikasi LoRa dan sebaiknya menggunakan sangkar cuaca sesuai standar., This study aims to develop a Wireless Sensor Networks (WSN) system using ESP32 microcontroller, LoRa, and BME280 sensor as an alternative option for meteorological observation with relatively low cost and helps in increasing the spatial resolution of weather parameters observation. WSN composed using a BME280 sensor connected to ESP32, and LoRa as a communication method. The system is equipped with website, android application, and a Command Line Interface-based control panel. The website provides graphical display of real-time observations. Android application is used for location tags, location tracking for moving observations, and real-time data monitoring. The control panel functions to control the system remotely using the internet. System reads and send the sensor value to the nearest nodes using LoRa to the base station, then upload it to the database. WSN system is tested to measure weather parameters, namely temperature, relative humidity, and air pressure. Observation of weather parameters is only a small example of the use of the WSN system, because its use can be for various environmental elements. The test results shows that the WSN system is functioning properly and can monitor the development of weather conditions at various observation points. Node configuration must be considered for smooth LoRa communication and it is recommended to use a weather cage according to standards.
Keyword: command line interface, esp32, firebase database, LoRa, sensor

Judul: Development of Microclimate Monitoring System for Multi-Greenhouse using Internet of Things
Abstrak: Suhu udara, kelembapan udara, dan intensitas cahaya merupakan parameter lingkungan mikro di dalam rumah tanaman yang berpengaruh terhadap pertumbuhan tanaman dan perlu dilakukan pemantauan. Saat ini, Internet of Things (IoT) merupakan teknologi yang mumpuni dan dapat digunakan untuk melakukan pemantauan lingkungan mikro rumah tanaman di berbagai lokasi secara langsung. Tujuan penelitian ini yaitu rancang bangun sistem pemantauan suhu nutrisi, suhu udara, kelembapan udara, dan intensitas cahaya pada berbagai rumah tanaman berbasis IoT. Penelitian ini menggunakan metode System Development Life Cycle (SDLC) yang terdiri dari: (1) analisis kebutuhan sistem, (2) desain sistem, (3) implementasi sistem, dan (4) pengujian sistem. Perangkat elektronika yang digunakan yaitu sensor DHT21, BH1750, DS18B20, dan mikrokontroler ESP32. Hasil perhitungan Root Mean Square Error (RMSE) dan Mean Absolute Percentage Error (MAPE) pada kalibrasi sensor yang rendah menunjukkan nilai eror data dari pembacaan sensor yang kecil. Desain sistem dibuat menggunakan rancangan database dan entity relationship diagram. Sistem diimplementasikan pada berbagai rumah tanaman yaitu dua greenhouse tipe standard peak dan satu plant factory. Berdasarkan hasil uji System Usability Scale (SUS), didapatkan nilai SUS 75,67 yang artinya sistem dapat diterima oleh pengguna dan termasuk kategori sistem yang baik pada kelas B. Kesimpulan dari penelitian ini yaitu sistem pemantauan lingkungan mikro pada berbagai rumah tanaman berhasil dilakukan menggunakan perangkat elektronika berbasis IoT yang dapat diakses oleh pengguna pada web server., Air temperature, air humidity, and light intensity are microclimate parameters in greenhouse that affect plant growth and must be monitored. Currently, the Internet of Things (IoT) is powerful and can be used to monitor microclimate parameters in multi-greenhouse at various locations directly. The purpose of this research is to design and develop a system for monitoring nutrient temperature, air temperature, air humidity, and light intensity in multi-greenhouse based on IoT. This research used the System Development Life Cycle (SDLC) method, which consists of: (1) system requirement analysis, (2) system design, (3) system implementation, and (4) system testing. The electronic devices used were DHT21, BH1750, DS18B20 sensors, and ESP32 microcontroller. The low values of Root Mean Square Error (RMSE) dan Mean Absolute Percentage Error (MAPE) obtained in sensor calibration indicate small data errors from sensors. The system design was made with a database design and entity relationship diagram. The system was successfully implemented in multi-greenhouse which two greenhouse standard peak type and a plant factory. Based on the results of System Usability Scale (SUS) test, the system received SUS score of 75.67, which means that system was acceptable to users and included good category in class B. The conclusion of this research was microclimate monitoring system in multi-greenhouse has been successfully implemented using electronic devices based on IoT that can be accessed by users on a web server.
Keyword: ESP32, Internet of Things, rumah tanaman, sensor lingkungan, sistem informasi

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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