Judul: Produksi Bioetanol dari Limbah Tanaman Jagung Melalui Sakarifikasi dan Fermentasi Simultan dengan Kultur Campuran Saccharomyces cereviseae dan Pichia stipitis
Abstrak: Corn crop waste as a lignocellulosic material has a large potential in Indonesia. Lignocellulosic material is a renewable raw material for ethanol production. Most of the composition of lignocellulisic materials consist of cellulose, hemicellulose, and lignin. Cellulose hydrolysis will produce glucose that can be fermented into ethanol by Saccharomyces cereviseae. Hemicellulose hydrolysis will produce xylose. Sacharomyces cerevisiae is the most applied and traditional microorganism for ethanol production. It has a high ethanol tolerance, and high yields and rates of fermentation, but its inability to ferment xylose, the second most abundant sugar in nature, limits its use in biofuel production. This study applies simultaneous saccharification and fermentation techniques to the production of ethanol from corn crop waste materials using a culture volume Saccharomyces ceseviseae, Pichia stipitis, and mixed culture of Saccharomyces cereviseae and Pichia stipitis.Simultaneous saccharification and fermentation with individual yeasts Pichia stipitis shows the best results with high ethanol at 5.84 g/l and yield 0.11. Saccharomyces cereviseae and Pichia stipitis mixtures with etnanol 4.34 g/l and yield 0.10, and Saccharomyces cereviseae at least with the results 3.79 g/l ethanolo and yield 0.07 at the time of 96 hours. The results of this study indicate that at 96 hours of mixed culture of Saccharomyces cereviseae and Pichia stipitis not improve the performance their single culture. Further research still needs to be done by optimizing the ratio and between the two yeasts and sequence inoculation.
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Judul: Produksi Bioetanol dari Limbah Tanaman Jagung Melalui Sakarifikasi dan Fermentasi Simultan Menggunakan Biakan Zymomonas mobilis dan Pichia stipitis
Abstrak: Bioethanol is renewable energy can produce from fermentation of sugar (Anuj et al., 2007). Bioethanol can be given from material that has lignocellulose. Indonesia has a pottential raw material that can be renewable. In Indonesia this material is plenitude and have a high componnent cellulose inside. The use of harvest stover has advantages there are not have competitive with food, price is cheap, and that material is so much in Indonesia (Rachamniah et al., 2009). Usually, cornstover only can be used to fuel (dirrect), feedstock, and most of not use and only be waste. This waste have a component such as cellulose 36.81%, hemicellulose 27.01%, lignin 15.07% and ash 6.04%. (Anonim, 2009). Use of Lignococellulotic only a part of cellulose, whreas 27.01% ingredients of cornstover is hemicellulose can be used to ethanol production. Use of hemicellulose hoped can increase concentration of ethanol. Hydrolysis process and fermentation will be effective and efficient if done continiusly without long time break or use simultaneous saccharification and fermentation process (SSF). In tis process use enzym cellulase, xylanase, β-glucosidase added for produce glucose and xylose. Microorganism that can used for produce ethanol such as Zymomonas mobilis and Pichia stipitis. Zymomonas mobilis can produce ethanol (5-10%) and productivity (2,5 times) higher than Saccharomyces cereviseae (Zhang et al., 1995 and Dien et al., 2003). Pichia stipitis can use pentose that can not use by Saccharomyces cererviseae and Pichia stipitis. The objectives of this research are to determine productivity of saccharification and fermentation process with measuring fermentation parameters, such as producing biomass, changing of pH, using of reduction sugar, using of total sugar, producing ethanol, the correlation of substrate consumption to the product or biomass, biomass to the product and efficiency for each microorganism. In this research, pretreatment process there are delignification, hydrothermal I and hydrothermal II. Delignification using 0,073 g/g cornstover. Condition temperature of delignification that is 74,6oC as long as 2 hour (Hakim, 2010). Condition temperature of hydrothermal I that is 121oC as long as 1 hour. Condition temperature of hydrothermal II that is 180oC as long as 20 minute. After that, cornstover that has pretreatment 6,67% (dw/v) mix with liquor substrate. Liquor substrate consist of liquor from pretreatment and buffer sitrate phosphate pH 5 with comparison 1:1. After that, hydrolysis enzym process done with added cellulase 7 IU/g cellulose and xylanse 3 IU/g hemicellulose. Condition temperature of hydrolysis enzym that is 50oC as long as 24 hour. After process hydrolysis enzim finished, simultaneous saccharification and fermentation done with the temperature condition that is 32oC, added nutrient fermentation that is urea (24%) has many 0,167% (v/v) (Thomsen et al., 2008). Hydrolysis enzym process done with added cellulase 7 IU/g cellulose, xylanse 3 IU/g hemicellulose, and β-glucosidase added has many 1 IU/g cellulose. The starter added that is 10% (v/v) (Samsuri et al., 2007). Starter that use from microorganism Zymomonas mobilis, mix of microorganism Zymomonas mobilis and Pichia stipitis and Pichia stipitis As long as simultaneous saccharification and fermentation going on, measuring done such as biomass, pH, reduction sugar and total sugar analysis.
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Judul: Metric Solution and Electromagnetic Field Tensor of Black Hole in 4+1 Dimensional Kerr-Newman Space-Time
Abstrak: Solusi metrik Kerr-Newman merupakan solusi paling umum untuk black hole. Konstruksi metrik pada dimensi 4+1 didasarkan pada asumsi bahwa hukum-hukum fisika berlaku di semua dimensi. Tujuan penelitian ini adalah untuk mengonstruksi solusi metrik Kerr-Newman 4+1 dimensi beserta tensor medan elektromagnetiknya. Dilakukan pengambilan tebakan, reverse engineering, analisis persamaan medan Einstein, manipulasi aljabar, dan pengambilan limitasi untuk mengonstruksi dan membuktikan solusi metrik dan tensor medan elektromagnetik. Terdapat komponen-komponen off-diagonal pada tensor metrik yang diperoleh. Hal ini berkaitan dengan keberadaan parameter momentum angular a. Keberadaan parameter muatan Q dan parameter momentum angular a pada black hole Kerr-Newman mengindikasikan keberadaan parameter magnetik yang muncul pada black hole Kerr-Newman. Keberadaan parameter magnetik berkontribusi dalam membentuk medan potensial yang diajukan, dimana medan potensial tersebut terbukti benar dengan menganalisis komponen-komponen tensor medan elektromagnetik yang diperoleh., Kerr-Newman metric solution is the most common solution of black hole. Metric construction on 4+1 dimensions based on the assumption that physics law valid in all dimension. The aims of this research is to construct Kerr-Newman metric solution in 4+1 dimension and it’s electromagnetic field tensor. Guesswork, reverse engineering, analysis of Einstein’s field equation, algebraic manipulation, and limitation taking are carried out to construct and prove metric solution and electromagnetic field tensor. There is off-diagonal components in metric tensor obtained. It is related to the existence of angular momentum parameter a. The existence of charge parameter Q and angular momentum parameter a in Kerr-Newman black hole indicates the existence of magnetic parameter which appear in Kerr-Newman black hole. Magnetic parameter existence is contribute in the forming of potential field which submitted, whereby this potential field is proven correct by analyzing electromagnetic field tensor components obtained.
Keyword: Einstein field equation, Electromagnetic field tensor, Kerr-Newman metric solution, Magnetic parameter