Judul: Optimalisasi Produksi Embrio Domba Secara In Vitro: Penggunaan Medium CR1aa dan Pengaruh Status Reproduksi
Abstrak: The aim of this study was to investigate the capacity of CR1aa as a simple medium for in vitro maturation, fertilization and culture of ovine embryo and to determine whether the ovarian reproductive status influenced the number of follicles, oocytes quality, nuclear maturation, fertilization and cleavage rates for ovine in vitro embryo production. In first experiment, oocytes were matured in Tissue Culture Medium (TCM)-199 as control and CR1aa as treatment. Both maturation medium were supplemented with 10% Fetal Bovine Serum (FBS), 10 IU/ml Follicle Stimulating Hormone (FSH), 10 IU/ml Luteinizing Hormone (LH), 1 ìg/ml Estradiol and 100 IU/ml penicillin-streptomycin. Matured oocytes were fertilized in BO or CR1aa medium, supplemented with 2.5 mM caffeine benzoate and 20 mg/ml heparin. Zygotes were cultured in TCM-199 or CR1aa medium, supplemented with 5% FBS, 5 mg/ml insulin, 100 IU/ml penicillin streptomycin. In second experiment, the pairs of ovary was classified into four groups: 1) ovaries with Corpus Luteum (CL) and Dominant Follicle (DF), 2) ovaries with CL, without DF, 3) ovaries with DF, without CL, 4) ovaries without both CL and DF. Results showed that in first experiment, the highest maturation rate was found in TCM -199 medium (73.27%) and significantly different (P<0.05) from CR1aa (52.88%). Fertilization rate in CR1aa medium (67.59%) was higher (P<0.05) than in BO medium (52.94%). Furthermore, there was no significant difference (P>0.05) between cleavage rate of ovine embryos in TCM-199 or CR1aa medium (39.45%; 43/109 vs 50.94%; 54/106). In second experiment, there was no significant different (P>0.05) in the number of follicles were found from all groups of ovaries. The number of oocytes with good quality from pairs of ovary with CL and DF was 7.94 ± 2.59, significantly different (P<0.05) with pairs of ovary with DF without CL (4.40 ± 1.50). The maturation rate of oocytes with good quality were 75.51% from pairs of ovary with CL and DF and significantly different (P<0.05) with pairs of ovary with DF without CL (42.86%). Fertilization and cleavage rates were similar (P>0.05) from all groups of ovaries. In conclusion, optimum result on ovine in vitro embryo production can be achieved from a combination of TCM -199 as maturation medium and CR1aa as fertilization and culture medium. Furthermore reproductive status of ovaries can influence in obtaining the number of good quality oocytes and maturation rate, but not in fertilization and cleavage rate.
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Judul: IPB (Bogor Agricultural University)
Abstrak: This research was designed in four experiments to determine the effectiveness of supplementation of various types and concenbations of sera and cocultures of Fallopian tube epithelial and cumulus cells in Tissue Cutture Medium 199 (TCM-199) on in vitro bovine embryo production. In the first experiment oocytes were collected by aspiration method from ovaries obtained from the slaughter house. Oocytes were classified into three grades (A, B and CID) according to their qualities. The second experiment dealt with maturation of cmcytes of A and B qualities (COCs, cumulus oocyte complexes) in TCM-199 supplemented with 5, 10, 15 and 20 percents of fetal bovine serum (FBS), estrous heifer serum (SHg) and men days after estrous heifer serum (SH7), respectively, at 38.5°C. 5.0% Cq2 in humidified air for 22 to 24 hours. Oocytes were thea fedid in vitro with capcitated spematozoa. After six to eight hours of culturing, the presumptive zygotes were tiansferred into 100 pl drop of in virro culture medium for development of embryos. These embryos were then incubated for 4x48 hours, evaluated and its culture medium was changed with a fresh one every 48 hours.
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Judul: Closed Knight’s Tour pada Papan Catur Berbentuk Persegi Panjang dengan Ukuran Maksimal 8 × 8
Abstrak: Closed Knight’s Tour merupakan langkah kuda yang mengunjungi setiap kotak pada papan catur tepat satu kali dengan langkah kuda berawal dan berakhir pada kotak yang sama. Graf yang terbentuk dari closed knight’s tour dinotasikan sebagai graf G(m,n), dengan m adalah banyaknya baris dan n adalah banyaknya kolom. Graf tersebut memuat Hamiltonian cycle. Sebuah graf terhubung G adalah graf bipartite jika dan hanya jika graf tersebut tidak memiliki cycle dengan panjang ganjil. Papan catur yang memenuhi syarat cukup untuk memiliki closed knight’s tour adalah papan catur berukuran 5 × 6, 5 × 8, 6 × 6, 6 × 7, 6 × 8, 7 × 8, dan 8 × 8.
Keyword: bipartite, closed knight’s tour, hamiltonian cycle, knight’s tour, teori graf