Judul: Pendekatan AIDA terhadap Hak-Hak Konsumen dan Konsumerisme di Kalangan Mahasiswa
Abstrak: Tujuan penelitian ini adalah menganalisis keterkaitkan diantara komponen AIDA yakni kesadaran, ketertarikan, keinginan, tindakan memperjuangkan hak-hak konsumen pada mahasiswa yang telah dan belum mengambil matakuliah perilaku konsumen dan menganalisis hubungan kesadaran tentang hak-hak konsumen dengan konsumerisme. Penelitian ini merupakan survei terhadap 204 mahasiswa IPB yang dikelompokkan atas mahasiswa yang telah dan belum mengambil matakuliah perilaku konsumen. Analisis data menggunakan independent t-test dan Structural Equation Modelling (SEM). Hasil penelitian menunjukkan terdapat perbedaan signifikan kesadaran dan tindakan memperjuangkan hak-hak konsumen antara mahasiswa yang telah dan belum mengambil matakuliah perilaku konsumen. Hasil penelitian juga menunjukkan bahwa ketertarikan berpengaruh positif signifikan terhadap keinginan, dan keinginan berpengaruh positif signifikan terhadap tindakan memperjuangkan hak-hak konsumen pada kedua kelompok.
Keyword: AIDA, hak-hak konsumen, konsumerisme, pendidikan konsumen

Judul: Analisis Perilaku Konsumsi Susu dan Hasil Olahannya pada Mahasiswa IPB dengan Metode AIDA dan CRI
Abstrak: Penelitian ini bertujuan menganalisis perilaku konsumsi susu Fapet dan hasil olahannya dengan model AIDA dan CRI. Contoh pada penelitian ini 209 mahasiswa aktif IPB semester 2, 4, dan 6 yang dipilih secara acak sederhana. Hasil penelitian menunjukkan terdapat hubungan antara kesadaran dan ketertarikan, ketertarikan dan keinginan sedangkan keinginan tidak memiliki hubungan dengan tindakan. Hasil penelitian juga menunjukkan bahwa jenis kelamin, uang saku, pekerjaan ayah, besar keluarga, kesadaran, ketertarikan, dan tindakan memiliki hubungan dengan frekuensi konsumsi susu Fapet dan hasil olahannya. Variabel kesadaran, ketertarikan, dan tindakan juga memiliki hubungan dengan jumlah jenis susu Fapet dan hasil olahannya yang dikonsumsi. Semakin tinggi kesadaran dan tindakan maka semakin tinggi frekuensi dan jumlah jenis susu Fapet dan hasil olahannya yang dikonsumsi. Berdasarkan hasil analisis Customer Response Index (CRI), respon mahasiswa terhadap produk susu Fapet dan hasil olahannya sebesar 78.9 persen menunjukkan bahwa strategi pemasaran yang dilakukan produsen telah efektif.
Keyword: AIDA, CRI, susu dan hasil olahannya, susu Fapet

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable