Judul: Analisis Keragaman Genetik Dan Filogenetik Populasi Hama Boktor Xystrocera Festiva (Coleoptera: Cerambycidae) Di Jawa
Abstrak: Banyak studi yang telah dilakukan untuk mencari sistem pengendalian hama boktor (Xystrocera festiva), yaitu hama utama pada tegakan sengon. Namun hingga saat ini sistem pengendalian yang ditemukan belum berhasil menangani serangan hama boktor. Hal ini disebabkan oleh minimnya informasi mengenai hama tersebut, salah satunya informasi mengenai variasi genetik pada hama boktor. Penelitian ini bertujuan untuk menganalisis pola sebaran serangan hama boktor pada delapan populasi sengon di Jawa, dan menganalisis variasi genetik larva yang ada di Jawa. Sampling dilakukan menggunakan metode purposive pada 8 populasi, yaitu Sukabumi, Cianjur, Tasikmalaya, Ciamis, Temanggung, Magelang, Kediri dan Malang. Variasi genetik boktor disekuen pada gen Cytochrome Oxidase subunit I (COI). Pola sebaran hama boktor di Jawa bervariasi di masing-masing populasi, yaitu beragamnya ukuran larva berdasarkan diameter kepala dan panjang tubuh yang mengindikasikan larva melakukan serangan berulang pada suatu populasi. Sekuen gen COI larva boktor yang berasal dari Jawa diperoleh sepanjang 605 pb yang terdiri dari 9 haplotipe yaitu 4 haplotipe diperoleh dari Jawa Barat, 3 haplotipe dari Jawa Tengah, dan 2 haplotipe dari Jawa Timur. Keragaman genetik X. festiva di Jawa termasuk kategori tinggi, yaitu Hd=0.772. Berdasarkan hasil analisis jarak genetik menunjukkan ilustrasi pohon filogenetik menunjukkan jarak genetik suatu populasi berbeda dipengaruhi oleh jarak geografis masing-masing populasi. Pohon filogenetik juga menunjukkan semua sekuen X. festiva berkelompok, dan berbeda dengan sekuen kelompok X. globosa yang diperoleh dari basis data Genbank. Hasil sekuen tersebut dapat digunakan sebagai barcode untuk membedakan spesies dari genus Xystrocera.
Keyword: Boktor, COI, populasi, sengon, variasi genetik

Judul: Struktur dan Keragaman Genetik Populasi Hama Boktor (Xystrocera festiva) pada Hutan Rakyat Sengon (Falcataria moluccana) di Jawa.
Abstrak: Boktor (Xystrocera festiva) is the most destructive sengon pests, because the larvae feeding activity is done by broaching the woody tissue inside sengon trees. The objective of this research was to study population structure and genetic diversity of boktor pests in Java using RAPD markers. Boktor larvae were sampled from 10 susceptible trees in eight districts in Jawa, and then census was done to calculate the total individuals found in each tree, before taking measurements of body length and diameter of its head. Having been census a number of 10 larvae per tree of various sizes were picked up as sample for DNA analysis. DNA extraction was done using CTAB (Cetyl Trimethil Ammonium Bromide) method, and the PCR (Polymerase Chain Reaction) was done using OPP 9, OPP 15, and OPP 19 primers to get RAPD (Random Amplified Polymophic DNA) fragment. Scored fragment data was analyzed using software POPGENE 1.31 version 32 and NTSYS version 2.0. The results showed the heterogeneity of the population structure according to the diameter and body length of each population. Result of RAPD analysis showed that the highest heterozigosity (He) value was from Tasikmalaya (0.2525) and the lowest heterozigosity (He) was 0.1039 from Magelang. Dendrogram based on genetic distance showed that Kediri population was closely related with Temanggung while Ciamis population is farthest.
Keyword: CTAB, RAPD, sengon, population structure, Xystrocera festiva

Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4