Judul: Karakteristik Semen Segar, Morfologi, dan Pengujian Keutuhan Membran Plasma Spermatozoa Kelinci Lop dan Rex
Abstrak: The study was conducted to investigate the quality of semen between Lop and Rex breed. Semen samples was collected by using artificial vagina and evaluated macro and microscopically. The evaluation included semen volume, pH, consistency, color, mass movement, motility, individual scores, concentration, and viability. The sperm morphology was examination using carbofuchsin eosin staining and the plasma membran integrity was determine using Hypo-Osmotic Swelling test (HOS test). Hypo-Osmotic solution was modified by mixing fructose and sodium citrate into three different osmotic pressure: 50, 100, and 150 mOsm/kg. All data were analyzed statistically using analysis of variance (ANOVA), whereas the data from HOS test were analyzed using completely randomized design (CRD) with three factor patterns. The result showed that mass movement, motility, and individual scores of Rex’s sperm was superior compare than Lop’s, but there were no difference in other parameters. The respons of plasma membrane integrity showed that Rex’s sperm was faster (15 minute) compare than Lop’s (30 minute). This fact may relate to the composition structure of the plasma membrane and seminal plasma.
Keyword: 

Judul: Penentuan Waktu Optimal Pengujian Keutuhan Membran Plasma Spermatozoa Semen Beku Sapi Limousin dan Frisien Holstein Menggunakan Hypo-Osmotic Swelling (HOS) Test.
Abstrak: The study was conducted to determine the optimal time to test the integrity of plasma membrane of Limousin and Frisien Holstein (FH) bull frozen semen spermpatozoa using hypoosmotic swelling (HOS) test. A total of 15 samples from each bull used. All samples were incubated in a hypoosmotic solution with osmolarity of 150 mOsm Kg-1 for 60 minutes. The hypoosmotic solution made by admix 1.351 g fructose, 0.735 Na-citrate and water until the volume was 100 ml. Every 15 minutes the whole sample was evaluated the percentage of spermatozoa that reacts to the solution. Optimal time was obtained at 30’ and 45’, both on Limousin and FH frozen semen. The mean percentage of reacted Limousin bull spermatozoa at 30’ and 45’ respectively were 53.90 ± 6.81 % and 52.47 ± 8.00 %. Whereas the FH bull spermatozoa was 44.52 ± 6.26 % and 44.46 ± 8.42 %. Spermatozoa from frozoen semen needs time to react to hypoosmotic solution due to the added of freezing diluent. This causes the spermaozoa did not react immediately to the hypoosmotic solution. Plasma membrane integrity is very important for sperm fertility. Fertility enzymes contained in the head of spermatozoa, thus if the plasma membrane of the head broken, spermatozoa will lose their fertility. Similarly in the tail, if the plasma membrane broken, spermatozoa will lose their motility.
Keyword: 

Judul: Strategi Pemberdayaan Masyarakat dalam Rangka Rehabilitasi TAHURA Bukit Soeharto
Abstrak: TAHURA Bukit Soeharto merupakan salah satu bentuk kawasan konservasi alam yang terletak di wilayah Kabupaten Kutai Kartanegara dan Penajam Paser Utara dengan luasan 61.850 ha. Dalam rangka pemanfaatan kawasan TAHURA Bukit Soeharto, ditetapkan 3 (tiga) Kawasan Hutan dengan Tujuan Khusus (KHDTK) yaitu : (1) KHDTK Wanariset Samboja seluas 3.504 ha ; (2) KHDTK Pendidikan dan Latihan Kehutanan (BDK) seluas 4.310 ha dan (3) KHDTK Penelitian dan Pendidikan bagi Universitas Mulawarman Pusat Penelitian Hutan Tropis Lembab (PPHT) seluas 20.271 ha.
Keyword: