Judul: Kajian Biofisik, Wisatawan dan Masyarakat Sekitar Lokawisata Baturaden Banyumas
Abstrak: The local government of Central Java Province sets up Banyumas Regency as tourism destination area and Lokawisata Baturaden is the most popular of all tourism object in Banyumas.
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Judul: Kajian biofisik wana wisata di kesatuan pemangkuan hutan Banyumas Timur dan kaitannya dengan pengunjung
Abstrak: Penelitian dilaksanakan di tiga Wana Wisata Kesatuan Pemangkuan Hutan Banyumas Timur yang secara administrasi pemerintahan terletak di Daerah Tingkat II Banyumas dan Cilacap, Propinsi Daerah Tingkat I Jawa Tengah. Penelitian ini bertujuan untuk mengidentifikasi faktor-faktor biologi dan fisik serta rnengetahui kaitan antara faktor-faktor biologi dan fisik kawasan wana wisata dengan pengunjung. Metode penelitian yang digunakan adalah dengan identifikasi. Vegetasi diidentifikasi rnelalui penelusuran bahan kepustakaan dan pengamatan lapang. Untuk rnengetahui jenis vegetasi tingkat sernak digunakan analisis vegetasi dengan rnetoda garis berpetak (Soerianegara dan Indrawan, 1982). Penelitian terhadap jenis-jenis satwa dengan mengkombinasikan metode pengamatan secara langsung dan tidak langsung. Pengarnbilan contoh pengunjung dengan cara purpc>sive samplir,g dan tehnik pengumpulan data dengan rnenggunakan kuisioner. Berdasarkan hasil penelitian faktor biologi, diketahui vegetasi yang ada di tiga wana wisata rnenunjukkan jumlah jenis yang tinggi, wana wisata Baturraden 29 mili dan 51 jenis, Gunung Selok 34 famili dan 58 sedangkan Curug Cipendok 33 famili dan 56 jenis. hasil analisis vegetasi tingkat semak pada kawasan wisata Baturraden, mempunyai rumput pait INP tertinggi 36.14 %. (Axonopus compressus) sedang pada Gunung Selok, INP jenis vegetasi penutup tanah berkisar antara 4.30 sampai 13.17%, dan pada Curug Cipendok, jenis yang mempunyai INP tertinggi adalah jenis rumput kawat (Cyno- don dactylon) yaitu 30.55. fa jenis Dari нада Satwa burung yang terbanyak ditemui adalah dari golongan (Aves). Di wana wisata Baturraden berjumlah 15 famili yang terdiri dari 18 jenis. Gunung Selok 21 fa- mili dan 27 jenis sedangkan di Curug Cipendok 15 famili dan 17 jenis. Hasil penelitian faktor fisik adalah Baturraden mempunyai aksesibilitas yang dingkan wana wisata Gunung Selok dan Jika ditinjau kelengkapan sarana dan wisata Baturraden wana wisata lainnya. wana tinggi wisata diban- Curug Cipendok.. prasarana, adalah lebih baik dibandingkan напа dua Hasil pengukuran debit air dari sungai yang alir di sekitar kawasan wana wisata meng Baturraden adalah 0.0067 m³/detik dan 0.0045 m³/detik dan untuk sungai yang mengalir di kawasan wana wisata Gunung Selok adalah 0.0120 m³/detik.
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Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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