Judul: Kesesuaian Ruang Terbuka Hijau pada Pola Ruang Menggunakan Citra SPOT-7 di Kecamatan Ciomas dan Tamansari Kabupaten Bogor
Abstrak: Pertumbuhan penduduk menyebabkan penggunaan lahan untuk permukiman dan lahan terbuka yang berdampak pada penurunan luas ruang terbuka hijau (RTH) di Kecamatan Ciomas dan Tamansari. Penelitian ini bertujuan menganalisis perubahan RTH menggunakan citra SPOT-7 tahun 2016 dan 2021 dan menganalisis kesesuaiannya dengan rencana tata ruang wilayah (RTRW) Kabupaten Bogor tahun 2016-2036. Luasan RTH pada tahun 2016 hingga 2021 di Kecamatan Ciomas dan Tamansari mengalami penurunan, tetapi luasan RTH tiap kecamatan masih memenuhi peraturan yang telah ditetapkan oleh pemerintah pada daerah perkotaan yaitu sebesar 30%. Kesesuaian RTH dengan RTRW di Kecamatan Ciomas didapat mencapai 64,04% pada tahun 2016 dan meningkat menjadi 67,33% pada tahun 2021, sedangkan di Kecamatan Tamansari nilai kesesuaian RTH dengan RTRW lebih tinggi sebesar 93,19% pada tahun 2016 namun turun menjadi 89,40% pada tahun 2021., Population growth causes the use of land for settlements and open land which has an impact on reducing the area of green open space (RTH) in Ciomas and Tamansari districts. This study aims to analyze changes in green open space using SPOT-7 imagery for 2016 and 2021 and analyze their suitability with the Bogor Regency regional spatial plan (RTRW) for 2016-2036. The area of green open space in 2016 to 2021 in the Ciomas and Tamansari districts has decreased, but the area of green open space for each district still meets the regulations set by the government in urban areas, namely 30%. The suitability of green open space with RTRW in Ciomas district reached 64,04% in 2016 and increased to 67,33% in 2021, while in Tamansari was higher at 93,19% in 2016 but decreased to 89,40% in 2021.
Keyword: spatial pattern, SPOT imagery, Bogor Regency Spatial Planning, green open space, land cover

Judul: Suitability of Green Open Spaces on Spatial Patterns Using SPOT-7 Imagery in Sukaraja and Babakan Madang Districts Bogor Regency
Abstrak: Perkembangan daerah urban yang cukup tinggi berpengaruh pada percepatan pembangunan daerah, dan mengakibatkan perubahan tutupan lahan. Perubahan tutupan lahan terkendali dengan adanya rencana tata ruang wilayah (RTRW). Penelitian ini bertujuan menganalisis perubahan tutupan lahan di Kecamatan Sukaraja dan Babakan Madang, serta analisis kesesuaian tutupan ruang terbuka hijau (RTH) dengan pola ruang RTRW Kabupaten Bogor. Analisis perubahan tutupan lahan dilakukan dengan mengklasifikasi secara visual menggunakan citra SPOT-7 tahun 2016 dan 2021. Hasil klasifikasi tutupan lahan memiliki nilai indeks kappa sebesar 0,91. Perubahan luas tutupan lahan menjadi lahan terbangun terus bertambah, sedangkan luas RTH menurun. Luas RTH mengalami penurunan sebesar 5,41% di Kecamatan Sukaraja dan 2,79% di Kecamatan Babakan Madang. Kesesuaian luas RTH dengan pola ruang RTRW di Kecamatan Sukaraja sebesar 93,71% pada tahun 2016 dan mengalami penurunan pada tahun 2021 menjadi 91,83%, sedangkan di Kecamatan Babakan Madang sebesar 97,87% pada tahun 2016 dan mengalami penurunan menjadi 97,14% pada tahun 2021. Luas RTH di Kecamatan Sukaraja dan Babakan Madang masih memenuhi aturan yang ditetapkan pemerintah yaitu minimal 30% dari luas wilayah perkotaan., The relatively high development of urban area has an effect on acceerating regional development, resulting in changes in land cover. Changes of land cover are controlled by having a regional spatial planning (RTRW). This study aims to analyze the suitability of green open space (RTH) cover with the spatial patterns of the Bogor Regency spatial plan. Analyze of land cover change was carried out by visual classification method using SPOT-7 imagery for 2016 and 2021. The results of land cover classification have a kappa index value of 0,91. Changes in land cover to built-up land have continued to increase, while the area of green open space decreased. The area of green open space decreased by 5,41% in Sukaraja District and 2,79% in Babakan Madang District. The suitability of the area green open space with spatial patterns in Sukaraja District was 93,71% in 2016 and decreased in 2021 to 91,83%, while in Babakan Madang District it was 97,87% in 2016 an decreased to 97,14% in 2021. The area of green open space in Sukaraja an Babakan Madang sub-district still meets the goverment’s regulations, namely a minimum of 30% of the urban area.
Keyword: green open space, index kappa, land cover change, regional spatial plan, SPOT-7 imagery

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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