Judul: Peran Lembaga Keuangan dalam Peningkatan Pendapatan Nelayan Pemilik Kapal di PPP Lempasing, Provinsi Lampung
Abstrak: Capital provider for fishermen consists of formal and non-formal financial institutions. In the last 6 years, fishermen in PPP Lempasing had been utilizing formal financial institutions as a result of the increasing fisheries business. The purpose of this study was to analyze fishermen’s income, to define the correlation between fishermen’s status, both as client or non-client, and their level of income, and to define the characteristic of financial institutions utilized by fishermen in PPP Lempasing. Result showed that the net fishermen’s income of cantrang was Rp20 860 394/month and payang was Rp12 893 958/month. Chi square test showed that there were no relation between fishermen’s status, both as client or non-client, and their levels of income. The most preferred financial institution were formal financial institutions (66.7%), followed by own capital 20.83% and Non-formal 12.5%. The fishermen prefer to borrow from the bank because they can fullfil the minimum requirements.
Keyword: 

Judul: Strategi Pembiayaan pada Masyarakat Nelayan dalam Mendukung Usaha Perikanan Tangkap di PPI Muara Angke
Abstrak: Fishermen are still experiencing difficulties in getting financing for fishing. It also occurs in PPI Muara Angke which the location is near the capital city of Indonesia. This study was conducted to identify the financing patterns on fishermen and determine the financing strategies that support capture fishery business in PPI Muara Angke. The method that is used is qualitative case study method. Financing pattern is identified by descriptive qualitative method. The results are pattern of own capital plus borrow, assisted by the boss/caterer and assisted by PUMP (Development Business of Mina Rural) program. SWOT analysis is used to determine financing strategies. The strategies are synergy the financing from non formal institution, utilize the strategic value of PPI Muara Angke to support capture fishery business and optimize the involvement of bank through financing program from government (include People Business Loan and PUMP).
Keyword: fishermen, financing strategies, financing patterns

Judul: Gene Editing  of Two Cyanide Synthesis-related Genes in Cassava using CRISPR/Cas9
Abstrak: Cassava (Manihot esculenta) contain toxic quantities of cyanogenic glucoside. Reducing the potentially toxic cyanogenic glucoside levels in cassava could be achieved by gene editing through Clustered Regularly Interspaced Short Palindromic Repeats – CRISPR associated protein 9 (CRISPR/Cas9), a high- precision genome editing technique in plants. Two cassava UDP- glucosyltransferase (UGT) paralogs, UGT85K4 and UGT85K5, are the genes catalyzing the synthesis of cyanogenic glucosides in plants. The study aimed to construct the CRISPR/Cas9 recombinant vector carrying single guide RNA (sgRNA) to edit UGT85K4 and UGT85K5 genes, and transform sgRNA CRISPR/Cas9 construct into KU50 cassava calli. Five sgRNAs were designed, considering the on/off target efficiency score and GC content. Those sgRNAs were ligated into pRGEB32 plasmid and introduced to competent Escherichia coli DH5a. The PCR colony and DNA sequencing were performed to verify the sgRNA insert. The amplicons showed the expected band size of recombinant vector (180 bp). Based on sequencing results, only one sgRNA was successfully ligated into the plasmid that will be used to edit the UGT85K5 gene. The sgRNA CRISPR/Cas9 construct in pRGEB32 plasmid was successfully transformed into Agrobacterium tumefaciens and introduced into KU50 cassava calli through co-cultivation. The overgrowth of Agrobacterium occurred after the co-cultivation phase.
Keyword: CRISPR/Cas9, Cyanogenic glucoside, Gene editing, sgRNA