Judul: Wanita pengusaha pada masyarakat matrilineal dan peranannya dalam kehidupan keluarga dan masyarakat luas
Abstrak: Penelitian ini berusaha memahami tentang kehidupan wanita pada masyarakat matrilineal(masyarakat Kinangkabau) yang bekerja sebagai pengusaha konfeksi dan dikaitkan dengan peranan mereka dalam kehidupan keluarga dan masyarakat luas, sert a dicoba menganalisa berdasarkan sejumlah pemikiran.
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Judul: Analisis Peran Gender dalam Budaya Matrilineal, Manajemen Keuangan, dan Kesejahteraan Keluarga di Provinsi Sumatera Barat (Kasus pada Keluarga Pengrajin Bordir dan Sulaman)
Abstrak: West Sumatera is identical to the Minangkabau culture. Minangkabau culture has a unique culture compared to other cultures, and the only cultures in Indonesia that adopted matrilineal system. This research aimed to analyze gender role in matrilineal system, financial management activity, and family well-being in embroidery industry in West Sumatera. This research conducted in two districts: Kabupaten Lima Puluh Kota; Kota Bukittinggi. The data was collected on April until June 2012. There were 100 families as the sample of this research that was chosen purposively (respondent is wife/mother). The criteria of sample family are: intact family, there is at least a child that still enrolls in school, and the wife is get involved in the embroidery industry. The results showed that gender roles in decision making and division of labor in family were in a moderate level of gender partnerships between husband and wife in the family activities. The family financial management applied in moderate level, while the application of gender partnership has a low level. The family subjective well-being was showed by moderate level of wife satisfaction. The business income of women, gender roles of family financial management, and rural or urban location were dominant by factors that influenced family objective wellbeing; while the number of children, and gender roles in division of labor negatively effected the objective family well-being. The number of children, family income, application of family financial management were dominant by factors that influenced family subjective wellbeing; while the gender roles in division of labor, and rural or urban location negatively effected the subjective family well-being.
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Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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