Judul: Pengetahuan dan Praktek Higiene, Sanitasi, dan Keselamatan Kerja serta Produktivitas Tenaga Pengolah Makanan di Pondok Pesantren
Abstrak: Penelitian bertujuan untuk menganalisis Pengetahuan dan Praktek Higiene, Sanitasi, & Keselamatan Kerja serta Produktivitas Tenaga Pengolah Makanan di Pondok Pesantren. Desain yang digunakan adalah cross sectional, dengan teknik purposive metode survei yaitu 24 subjek tenaga pengolah makanan di 6 pondok pesantren di Depok. Hasil penelitian menunjukkan bahwa Pondok Pesantren AHA memiliki skor laik higiene sanitasi yang baik yaitu 90.2% tanpa uji laboratorium. Tingkat pengetahuan higiene dan sanitasi tenaga pengolah makanan pondok pesantren di Depok masuk ke dalam kategori sedang (79.2%) dan tingkat pengetahuan keselamatan kerja masuk ke dalam kategori baik (54.2%). Sebagian besar tenaga pengolah makanan di keenam pondok pesantren memiliki praktek higiene sanitasi yang baik (62.5%) dan sebanyak 45.8% tenaga pengolah makanan masuk ke dalam kategori sedang untuk praktek keselamatan kerja. Berdasarkan uji Spearman dan Pearson, tidak terdapat hubungan yang signifikan (p>0.05) antara karakteristik tenaga pengolah dengan pengetahuan higiene sanitasi dan keselamatan kerja, serta tidak terdapat hubungan (p>0.05) antara tingkat pengetahuan dengan praktek higiene sanitasi dan keselamatan kerja.
Keyword: Pengetahuan Higiene Sanitasi dan Keselamatan Kerja, Praktek Higiene Sanitasi dan Keselamatan Kerja, Produktivitas Kerja

Judul: Knowledge, Attitudes, and Practices of Food Handlers' Hygiene and Sanitation at Tegalega Park, Bandung City
Abstrak: Keracunan pangan dapat terjadi akibat konsumsi pangan siap saji dari pedagang kaki lima yang tercemar mikroba patogen. Tujuan dari penelitian ini adalah mengukur tingkat pengetahuan, sikap, dan praktik higiene dan sanitasi penjamah pangan di lingkungan Taman Tegalega Kota Bandung, dan melihat korelasi antar pengetahuan, sikap dan praktik, serta merumuskan rekomendasi perbaikan. Metode yang digunakan adalah survei terhadap 154 orang penjamah pangan. Hasil penelitian menunjukkan bahwa penjamah pangan telah memiliki pengetahuan, sikap, dan praktik higiene dan sanitasi yang baik (skor =70%) yang ditunjukkan masing-masing sebanyak 53,90% responden untuk pengetahuan, 54,55% untuk sikap, dan 57,79% untuk praktik higiene dan sanitasi. Karakteristik responden (usia, tingkat pendidikan, pengalaman bekerja, dan riwayat penyuluhan) berkorelasi positif terhadap pengetahuan, sikap, dan praktik higiene dan sanitasi. Korelasi antara sikap terhadap praktik cenderung lebih kuat dibanding pengetahuan terhadap sikap dan praktik. Secara keseluruhan masih perlu dilakukan peningkatan pengetahuan, sikap, dan praktik baik melalui penyediaan dan pemerataan fasilitas kebersihan maupun penyuluhan/pelatihan., Food poisoning can occur due to consumption of ready-to-eat food from street vendors contaminated with pathogenic microbes. The purpose of this study is to measure the level of knowledge, attitudes, and practices of hygiene and sanitation of food handlers in the Tegalega Park area, Bandung City, to see the correlation between knowledge, attitudes, and practices, and to formulate recommendations for improvement. The method used was a survey of 154 food handlers. The results showed that food handlers had good knowledge, attitudes, and hygiene and sanitation practices (score =70%) which were shown by 53.90% of respondents for knowledge, 54.55% for attitudes, and 57.79% for hygiene and sanitation practices. Respondent characteristics (age, education level, work experience, and experience of training) were positively correlated with knowledge, attitudes, and hygiene and sanitation practices. The correlation between attitudes toward practices tended to be stronger than knowledge toward attitudes and practices. Overall, it is still necessary to improve knowledge, attitudes, and practices through the equal distribution of cleaning facilities as well as counseling/training.
Keyword: higiene sanitasi, pengetahuan, Sikap, Praktik, penjamah pangan

Judul: Mitochondrial DNA Gene Organization and Phylogenetic Relationship of Naja siamensis
Abstrak: Naja siamensis is one of Asiatic cobra in the Family of Elapidae, known as a cryptic snake. It has many morphological variations that lead to misidentification. Misidentification can cause the wrong concept in developing antivenom types due to high snakebite cases occur in Thailand. Thus, an accurate species identification using a molecular approach is crucially required. Mitochondrial DNA has been used widely in snake molecular studies. These studies were aimed to determine the arrangement of the N. siamensis partial mitochondrial genes using reptile-oriented primers and to reveal the phylogenetic relationship among other species of Asian cobra. DNA extraction was carried out based on the standard DNA salting-out protocol and was conducted by bioinformatics analysis using BLASTn and MEGA 6 software. Gene annotation was performed against N. kaouthia complete genome. A total of 3121 bp of N. siamensis mtDNA genes were sequenced consisting of partial of six regions: 16S rRNA, ND1, tRNA-Ile, CRII region, tRNA-Leu, tRNA-Gln, and COI genes. The mtDNA gene organization of N. siamensis was predicted to has a similar pattern with the common Elapidae mtDNA gene organization. This study showed that there is a monophyletic form in Naja genus and N. kaouthia is the closest species with N. siamensis based on partial 16S rRNA and ND1 gene.
Keyword: 16S rRNA, Elapidae, ND1, phylogenetic relationship