Judul: Characteristic of Nucleotide Sequence of COI Gene of Blue Swimming Crab Portunus pelagicus (Linnaeus, 1758) for Fisheries Management in WPP-NRI 711
Abstrak: Sumberdaya perikanan Indonesia yang bersifat multispesies dapat menimbulkan fenomena cryptic dan complex species, sehingga dibutuhkan metode yang akurat dalam pemastian spesies rajungan, yaitu dengan DNA barcoding. Penelitian ini bertujuan menentukan kepastian spesies dan menganalisis kondisi populasi rajungan (Portunus pelagicus) di perairan WPP-NRI 711 berdasarkan gen COI. DNA contoh rajungan berjumlah 10 individu yang didapatkan dari tiga lokasi, yaitu perairan Bintan, Bangka, dan Ketapang telah diisolasi. Kualitas untaian DNA yang baik digandakan dan diurutkan pada proses sekuensing. Seluruh contoh rajungan tervalidasi secara genetik sebagai spesies Portunus pelagicus. Komposisi basa nukleotida dari contoh rajungan didominasi oleh pasangan basa adenin-timin. Keragaman genetik rajungan di perairan Ketapang dan Bintan tergolong tinggi, sedangkan di perairan Bangka tergolong sedang. Ketiga lokasi penelitian memiliki nilai Fst yang tergolong rendah. Hasil penelitian menunjukkan bahwa populasi rajungan berada pada kondisi yang baik., Indonesia's multi-species fisheries resources can cause cryptic and complex species phenomena, so an accurate method is needed to determine the blue swimming crab species, such as DNA barcoding. This study aimed to determine the species certainty and to analyze the population status of blue swimming crab (Portunus pelagicus) based on the COI gene in WPP-NRI 711. A total of 10 blue swimming crab samples DNA obtained from three locations such as Bintan, Bangka, and Ketapang waters was isolated. High quality DNA strands are duplicated and sequenced. All of the samples have been genetically validated as Portunus pelagicus species. The nucleotide base composition of the COI gene from samples was dominated by adenine-thymine pairs. The genetic diversity value in Ketapang and Bintan are high, while in Bangka is moderate. All sampling sites have a low fixation index value. It can be concluded that the blue swimming crab populations are in a good condition.
Keyword: blue swimming crab, COI gene, molecular analysis

Judul: Morphometric diversity of blue swimming crab Portunus  pelagicus (Linneaus 1758) in water of Sunda Strait and South of Java for  Fisheries Management Purpose
Abstrak: Rajungan hidup di perairan pesisir dengan substrat berpasir atau berlumpur. Perbedaan lingkungan perairan dan intensitas penangkapan mempengaruhi keragaman karakter morfometrik sebagai bentuk adaptasi dan mencirikan unit stok. Tujuan penelitian ini adalah untuk menganalisis keragaman morfometrik rajungan di Selat Sunda (mewakili WPP NRI 572) dan Selatan Jawa (mewakili WPP NRI 573). Pengambilan sampel rajungan di tiga lokasi yang berbeda yaitu Labuan (hasil tangkapan di Selat Sunda), Cilacap dan Banyuwangi (hasil tangkapan di Selatan Jawa). Pengukuran karakter morfometrik dengan metode konvensional dan Truss Network Analysis (TNA). Analisis hubungan lebar karapas dengan bobot menunjukkan bahwa rajungan di ketiga lokasi memiliki pola pertumbuhan masing-masing adalah isometrik, alometrik negatif, dan alometrik positif. Hasil uji kruskal wallis menunjukkan bahwa terdapat beberapa karakter morfometrik yang berbeda nyata pada rajungan jantan dan betina. Analisis kluster menunjukkan bahwa rajungan di Cilacap dan Banyuwangi memiliki kemiripan yang lebih tinggi dibandingkan dengan rajungan Labuan. Hasil analisis diskriminan menunjukkan bahwa populasi rajungan di Labuan berbeda dengan populasi rajungan di Cilacap dan Banyuwangi. Perbedaan karakter morfometrik menunjukkan bahwa potensi rajungan di ketiga lokasi memiliki lebih dari satu unit stok, sehingga diperlukan pengelolaan yang berbeda antara rajungan di Selat Sunda dengan rajungan di Selatan Jawa., Blue swimming crabs (BSC) inhabit sandy or muddy substrates of coastal waters. Variations of the aquatic environment and fishing intensity might affect on morphological characters of spesies to facilitate adaptation and both might characterize stock unit as well. This study was aimed at analyzing morphometric diversity of blue swimming crab in water of Sunda Strait (representative of Fisheries Management Area (FMA) 572 of Indonesia) and South of Java (representative of FMA 573 of Indonesia). The crab samples were taken from three different locations, Labuan (catches in the Sunda Strait), Cilacap, and Banyuwangi (catches in the South of Java). Convensional method and Truss Network Analysis (TNA) were applied on morphometric character measurement. Analysis of the relationship between carapace width with weight showed that blue swimming crab in those three locations showed isometric, negative allometric, and positive allometric growth patterns. Result of the kruskal wallis test showed that morphometric characters were significantly different between male and female individuals. Cluster analysis showed that crabs of Cilacap and Banyuwangi showed high similarities which tended to be close population, while Labuan’s crabs were seemed to be dissimilar. Result of the discriminant analysis showed that the blue swimming crab population in Labuan was different from the populations blue swimming crab in Cilacap and Banyuwangi. Therefore, differences in morphometric characters indicate that the potential of the blue swimming crab in those three locations were originated from more than one stock unit, so that different management is needed between BSC in the Sunda Strait and South of Java.
Keyword: FMA 572 and 573, morphometrics, Truss-Network-Analysis, stock unit

Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4