Judul: Pemurnian Parsial dan Karakterisasi Beta-Galaktosidase Lactobacillus plantarum B 123
Abstrak: Lactose intolerance was a problem faced by a large proportion of the world's population. These problem could be alleviated by hydrolyzing lactose to glucose and galactose by β-galactosidase. β-Galactosidase occurred widely in nature and it was produced by a number of microorganisms. However, their characteristics were very different and it varied with the source of microorganism. The aim of this study was purification and characterization of β-galactosidase L. plantarum B 123. β-Galactosidase was extracted and partially purified by ammonium sulfate precipitation and dialysis. These steps resulted a purification level of 7.61 fold, a yield of 10.67%, and a specific activity of 61.53 U/mg protein. Optimum temperature and pH for dialyzed β-galactosidase were optimized at 50 °C and 7.0, respectively. Metal ions of Mg2+, Mn2+, Co2+, and Zn2+ in 10 mM stimulated the activity of crude β-galactosidase, while metal ions of Cu2+, Hg2+, and Ca2+ in 10 mM inhibited the activity of crude β-galactosidase. KM and Vmax for this dialyzed β-galactosidase were 1.13 mM and 7.25 U/mL, respectively.
Keyword: Bogor Agricultural University (IPB), characterization, dialysis, Lactobacillus plantarum B 123, β-galactosidase

Judul: Potensi Enzim -Galaktosidase dari Lactobacillus plantarum Strain B123 Indigenous untuk Hidrolisis Laktosa pada Susu UHT
Abstrak: Lactose concentration in milk is 4.6% (w/w) in average (Walstra 2006). The content of lactose with less than 1% (w/w) of final product is acceptable for people with lactose intolerance problem (Tuure and Corpela 2004). Enzymatic hydrolysis of lactose is one of the important biotechnological processes in the milk industry because of some beneficial effects including the elimination of lactose in milk for people with lactose intolerance, prevention of lactose crystallization and contribution to the product sweetness (Jurado et al, 2002). -Galactosidase enzyme can be produced by lactic acid bacteria. In this study, indigenous Lactobacillus plantarum strain B123 was isolated from Indonesian traditional fermented vegetable product. The use of this local potential strain was to increase its role to the added value products such as low lactose UHT milk. Even though low lactose UHT milk product has not been commercially marketed yet in Indonesia, however the need of low lactose milk for people with lactose intolerance was already present. This research was aimed to: (1) determine the growth condition of indigenous Lb. plantarum strain B123 to produce β-galactosidase (2) determine the production condition of -galactosidase from Lb. plantarum B123 strain with partial purification and (3) determine the potential of β-galactosidase from indigenous Lb. plantarum B123 strain to hydrolyze lactose of full cream and low fat-UHT milks, containing total fat 3.5 and 1.0% (w/v) respectively, in batch process. The result showed that the growth condition of indigenous Lb. plantarum B123 in MRSB to produce β-galactosidase were 2% inoculum, 1% of lactose, medium pH 8, and 24 h incubation time at 37°C. The enzyme showed an optimum activity to hydrolyzed oNPG substrate at pH 6.5 and temperature 50°C . Specific activity of -galactosidase has increased during the partial purification given 15.05 U/mg in crude enzyme to 109.58 U/mg in dialysed enzyme (at first production) and 87.09 U/mg in crude enzyme to 109.58 U/mg in dialysed enzyme (at second production). Lactose in full cream-UHT milk was hydrolyzed at a maximum rate of 60.0% (lactose concentration from 3.82% w/v to 1.52% w/v) and in low fat-UHT milk 81.1% (lactose concentration from 4.44% w/v to 0.84% w/v) by 7.2 U β-galactosidase/mL milk for 9 h at 50°C. Enzyme concentration, hydrolysis time and total fat concentration had a significant influence on the hydrolysis of lactose in UHT milk (p<0.05). According to the different analytical methods to monitor the lactose hydrolysis, a correlation between the results of glucose concentrations in full cream-UHT milk analyzed by GOD-POD kit and HPLC was observed. There was a positive correlation between analysis of glucose concentrations in full cream-UHT milk with GOD-POD kit and HPLC (r = 0.923).
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Judul: Warna bulu kucing ras Persia : variasi pola warna dan pewarisannya
Abstrak: Kucing Persia merupakan kucing ras yang paling umum dijumpai di Indonesia. Kucing Persia memiliki bulu yang panjang dengan variasi warna bulu yang sangat beragam. Perkawinan yang lebih terarah sangat perlu dilakukan untuk meningkatkan kualitas genetik warna dan pola warna bulu kucing. Penelitian ini bertujuan untuk mempelajari variasi warna dan pola warna bulu kucing Persia yang terdapat di Indonesia, yang dilakukan di kantor pusat Cat Fancy Indonesia mulai bulan Oktober 2000 sampai Januari 2001. Data yang digunakan merupakan data pedigree kucing Persia yang tercatat di Cat Fancy Indonesia dari tahun 1997 sampai Oktober 2001 yang terdiri dari 187 jantan dan 258 betina, selain itu juga dilakukan pengamatan langsung kepada tiga penyayang dan breeder kucing yang ada di Bandung. Secara umum tercatat 104 penyayang dan breeder kucing ras di Indonesia, 74,04% terdapat di Jawa Barat dan sisanya (25%) terdapat di daerah Jakarta. Jumlah kucing Persia yang lahir di Indonesia adalah 387 kucing (87,16%), yang terdiri dari 163 (42,12%) jantan dan 224 (57,88%) betina. Jumlah kucing Persia yang diimpor ke Indonesia adalah sebanyak 57 (12,845%) yang terdiri dari 23 (40,35%) jantan dan 34 (59,65%) betina. Jumlah kucing Persia yang terdapat di Jawa Barat (84,14%) lebih banyak dibanding jumlah kucing Persia yang ada di Jakarta (14,86%). Secara umum warna bulu cream (9,01%) dan pola warna solid (34,01%) memiliki persentase tertinggi. Warna cream merupakan warna dilusi dari warna merah (red) yang dapat dihasilkan dari berbagai macam kombinasi warna bulu yang dimiliki tetua sebelumnya, sebagai contoh dari perkawinan tetua yang memiliki warna bulu cream dengan red smoke. Perkawinan antara tetua yang memiliki warna dan pola warna bulu yang tidak murni dapat menghasilkan turunan yang memiliki warna dan pola warna bulu yang bervariasi.
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