Judul: Clustering Menggunakan Self Organizing Maps (Studi Kasus: Data Perkembangan Anak di Kabupaten Bogor)
Abstrak: Child development data which were gathered by the team from the Department of Family and Consumer Science, Bogor Agricultural University, require data processing to assess the characteristics of child development in Kabupaten Bogor. The purpose of this research is to implement the Self Organizing Maps (SOM) algorithm for data clustering and to obtain the characteristics from the clustering results. The data were obtained from 71 childrens at 2.5–3.4 years of age, 97 childrens at 3.5–4.4 years of age, and 126 childrens at 4.5–5.4 years of age. The data consist of four attributes: Cognitive, Language, Gross Motor, and Fine Motor. These data were the input for SOM algorithm. SOM clustering result was validated using Davies-Bouldin Index. The research shows that the clustering result for children at 2.5–3.4 years of age is 3 clusters, 3.5–4.4 years of age is 4 clusters, and 4.5–5.4 years of age is 3 clusters.
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Judul: Kajian Self-Organizing Maps (SOM) dalam pengelompokan objek (studi kasus: pengelompokan desa/kelurahan di Kabupaten Wajo Sulawesi Selatan)
Abstrak: Clustering is a process of classifying objects into groups which have similarity. The result of clustering will show that objects in one cluster will be more homogeneous than others. There are two methods in classic clustering analysis i.e. hierarchical cluster method and non-hierarchical cluster method. Determination of the number of clusters which formed by them is done subjectively. The cluster other methods also developed by using artificial intelligence. Artificial neural network is an information processing paradigm that inspired by the biology systems, it is neuron. Like brain which process information. Self-organizing maps (SOM) is one of the topology of Unsupervised Artificial Neural Network (Unsupervised ANN) which process does not require monitoring in his training. Application clustering using SOM algorithm is expected to be used as a tool to analyze the data in order to obtain the characteristics of the data that will be grouped. Clustering is used to group the data naturally without based on the specific class target. In this study, SOM compared with clustering method with large data sizes, it was two-step cluster. According to Bacher (2004), two-step cluster method (TSC) was a cluster method which can resolve the problem clustering measurement scale, especially for large data with variables which have categorical and numerical data types. Performance clustering SOM and two-step cluster method compared by the simulation data, afterwards, applying the method of SOM on clustering villages/urbans in Wajo regency, South Sulawesi. The data in this study consisted of two sources i.e. simulated data and secondary data. Simulated data was generated data multivariate distribution (μ,Ʃ) which useful to measure the performance of two-step cluster method and SOM in classifying an object. Secondary data, which used in this study, BPS’s data in Wajo regency, South Sulawesi, was Village Potential Data (VPD) in 2011. Simulation data was the generated data numeric type (M) which consisted of three forms of the population i.e. a). a population consisted of three clusters were clearly separated, b). a population which consisted of three clusters of overlapping (overlap) each other in small numbers, and c). a population that consisted of three clusters of overlapping (overlap) each other in large numbers. The results of methods SOM and TSC showed that simulation data has the good ability to classify data, however, TSC provides better clustering results for large data sizes than SOM. In addition, it is also showed that the larger the number of data, the misclassification of SOM would become larger, nevertheless, the changes were relatively smaller. In the other hand, the larger the number of data the misclassification of TSC method was become smaller. Secondary data, which used in this study, written documentation and identification of used variables about areas/villages in Wajo regency, South Sulawesi, was Village Potential Data (VPD) in 2011 i.e. : X1 (total population), X2 (family farm), X3 (family farm laborer), X4 (family power user), X5 (fuel for daily cooking), X6 (educational facilities), X7 (health personnel), X8 (population mortality). Objects in this research were applied around the village/urbans in Wajo district. The results of clustering with SOM method, village/urbans in the Wajo regency produced
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Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable