Judul: Stabilitas Sisipan Kandidat Gen Toleran Aluminium (B11) pada Padi Transgenik Asal IR64 Hasil Infeksi In-Planta
Abstrak: Rice variety IR64 is categorized as a sensitive variety to Aluminum (Al) toxicity. Development of Al-tolerant rice varieties can be done by inserting Al-tolerant gene, which one of them is B11 gene that has been succesfully isolated from Al-tolerant rice genotipe, Hawara Bunar. The B11 gene has been succesfully introduced to plant rice variety IR64 through in-planta technique and produced T1 generation. The objective of this research was to evaluate the stability of B11 gene insertion on T1, T2, and T3 generation derived from IR64 transformed variety through antibiotic selection, molecular analysis, and plant phenotipic observation. Antibiotic selection was done using hygromycin antibiotic with concentration of 20 mg/l. Gene insertion analysis was done with Polymerase Chain Reaction (PCR) technique. The result showed that B11 gene had been integrated in T0, T1, and T2 generation of IR64 transgenic plant. The B11 gene integration in T0 did not cause a chimera. The antibiotic selection showed that antibiotic-tolerant and sensitive ratio of T1 and some T2 and T3 populations was 3:1. Some of the T2 and T3 generation populations showed homozigous on B11 gene. The vegetative and reproductive characters of transgenic plant were not different to the wild tipe
Keyword: transgenic rice, IR64, B11 gene, Acid soil

Judul: Seleksi Tanaman Padi Transgenik Generasi T1 dan T2 yang Membawa Kandidat Gen Toleransi Aluminium
Abstrak: The use of aluminum (Al) tolerant rice variety is one of the alternative solutions to solve the food crop production problem in acid soils. The Al tolerant rice variety can be developed by genetic engineering. The T1 and T2 generation of transgenic rice has been developed from the transformation of rice using B11 gene, which was an Al tolerance gene candidate. However the true transgenic rice carrying the B11 gene has not been confirmed yet. This research was conducted to select and verify the T1 and T2 transgenic rice which contained the Al tolerant gene candidate. The result showed that the selection media containing paromomycin antibiotic could select the putatif transgenic rice without albinism effect that appeared in selection using canamycin antibiotic. The selection obtained true transgenic lines. The PCR analysis using the primers developed from nptII gene showed a 600 bp DNA band in the transgenic plant, but it was none in the wild type. The plant height and number of tiller characters were not significantly different between the wild type and the transgenic plant.
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Judul: Penggunaan Teorema Homeomorphy 2-Manifold dan Teorema Euler Poincare pada Torus 𝑇�� dan Simplicial Complex 𝐾��
Abstrak: Two dimensional topological spaces are said to be homeomorphic if they have the same topological invariant, where one of topological invariant used is an Euler characteristic. Homeomorphy 2-Manifold’s Theorem and Euler Poincare’s Theorem are used to distinguish two topological spaces. Homeomorphy 2-Manifold’s Theorem uses Euler characteristic to identify two dimensional topological spaces. Euler Poincare’s Theorem is an alternative way to find Euler characteristic with Betti number, which is topological invariant as well. The objective of this paper is to investigate the homeomorphism of torus 𝑇����� and simplicial complex 𝐾�����. Topological space torus and simplicial complex 𝐾����� have the same Euler characteristic, which is two. Based on Homeomorphy 2-Manifold’s Theorem topological space torus 𝑇����� and simplicial complex 𝐾����� is homeomorphic.
Keyword: simplicial, torus, Euler, homeomorphism, topology