Judul: The Relationship between Eating Habits, Physical Activity, and Exercise Habits with The Incidence of Dysmenorrhea in Female Adolescent at Pariaman City
Abstrak: Remaja putri mengalami perkembangan biologis ditandai dengan menstruasi yang dapat menyebabkan berbagai masalah termasuk dismenorea. Penelitian ini bertujuan untuk menganalisis hubungan kebiasaan makan, aktivitas fisik, dan kebiasaan olahraga dengan kejadian dismenorea pada remaja putri di Kota Pariaman. Penelitian dilakukan pada bulan Desember 2022 di SMAN 1 Kota Pariaman pada 94 orang subjek dengan desain cross-sectional study. Subjek dipilih secara purposive dengan pengumpulan data primer melalui kuesioner dan wawancara. Tingkat kecukupan zat gizi energi (33%) dan lemak (52,1%) masih tergolong defisit berat, sedangkan tingkat kecukupan zat gizi protein (36,2%) dan karbohidrat (29,8%) tergolong berlebih. Sementara itu, tingkat kecukupan zat gizi mikro yaitu kalsium (56,4%), zat besi (92,6%), dan vitamin C (70,2%) sudah tergolong dalam kategori cukup. Aktivitas fisik sebagian besar subjek tergolong dalam kategori sedang (64,9%). Sebanyak 46,8% subjek sering berolahraga dengan frekuensi 1 kali seminggu (40,9%). Sebagian besar durasi olahraga subjek sudah tergolong cukup (63,6%). Hasil analisis menunjukkan tidak terdapat hubungan yang signifikan antara tingkat kecukupan energi, lemak, karbohidrat, kalsium, zat besi, dan vitamin c, aktivitas fisik, dan kebiasaan olahraga dengan kejadian dismenorea (p>0,05). Namun, tingkat kecukupan protein dengan kejadian dismenorea menunjukkan hubungan yang signifikan (p=0,027)., Female adolescent experience biological development marked by menstruation which can cause various problems including dysmenorrhea. This study aims to analyze the relationship between eating habits, physical activity, and exercise habits with the incidence of dysmenorrhea in female adolescent at Pariaman City. The research was conducted in Desember 2022 at SMAN 1 Pariaman City on 94 subjects with a cross-sectional study design. Subjects were selected purposively by collecting primary data through questionnaires and interview. Adequacy levels of energy (33%) and fat (52,1%) are still classified as severe deficits, while protein (36,2%) and carbohydrates (29,8%) are classified as excess. Meanwhile, the level of adequacy of micronutrients, namely calcium (56,4%), iron (92,6%), and vitamin c (70,2%) was classified as sufficient. The physical activity of most of the subjects was in the moderate category (64,9%). As many as 46,8% of subjects often exercise with a frequency of 1 time a week (40,9%). Most of the subject’s exercise duration were classified as sufficient (63,6%). The results of the analysis showed that there was no significant relationship between the level of adequacy of energy, fat, carbohydrates, calcium, iron, and vitamin C, physical activity, and exercise habits with the incidence of dysmenorrhea (p> 0,05). However, the level of protein adequacy with the incidence of dysmenorrhea showed a significant relationship (p=0,027).
Keyword: dysmenorrhea, eating habits, exercise habits, female adolescent, physical activity

Judul: The Correlation between Emotional Eating, Physical Activity, and Quality of Diet with Primary Dysmenorrhea among College Student at PKU IPB
Abstrak: Dismenorea merupakan salah satu keluhan menstruasi yang kejadiannya cukup tinggi dan dapat memengaruhi produktivitas. Penelitian ini bertujuan mengetahui dan menganalisis hubungan emotional eating, aktivitas fisik, dan kualitas diet dengan dismenorea primer pada mahasiswi PKU IPB. Penelitian ini menggunakan desain cross-sectional study dengan jumlah 80 subjek. Penelitian dilaksanakan pada bulan Maret 2023 di Asrama Putri PKU IPB dengan pengukuran tinggi dan berat badan, wawancara, serta pengisian kuesioner. Instrumen pengukuran emotional eating menggunakan Dutch Eating Behavior Questionnaire-Emotional (DEBQ-E), kualitas diet menggunakan Indonesian Healthy Eating Index, dan dismenorea menggunakan Multidimensional Scoring System For Dysmenorrhea. Mayoritas subjek mengalami dismenorea primer (77,5%) dengan derajat ringan (53,7%). Mayoritas subjek tergolong sebagai non emotional eater (56,3%), memiliki tingkat aktivitas fisik yang ringan (58,7%), serta kualitas diet yang tergolong need improvement (86,2%). Tidak ada hubungan antara status gizi, emotional eating, aktivitas fisik, dan kualitas diet dengan keadaan dismenorea (p>0,05), tetapi terdapat hubungan signifikan antara riwayat dismenorea ibu dengan keadaan dismenorea (p=0,045). Tidak ada hubungan antara riwayat dismenorea ibu, emotional eating, aktivitas fisik, dan kualitas diet dengan derajat dismenorea (p>0,05), tetapi terdapat hubungan signifikan antara status gizi dengan derajat dismenorea (p=0,029)., Dysmenorrhea is a menstrual cramp that has a high incidence and can affect productivity. This research aims to determine and analyze the relationship between emotional eating, physical activity, and quality of diet with primary dysmenorrhea among college students at PKU IPB. The design of this research was a cross-sectional study with a total of 80 subjects. The research was conducted in March 2023 at PKU IPB Girls Dormitory by measuring height and weight, interviewing, and filling out questionnaires. The instrument for measuring emotional eating using the Dutch Eating Behavior Questionnaire-Emotional (DEBQ-E), quality of diet using the Indonesia Healthy Eating Index, and dysmenorrhea using the Multidimensional Scoring System for Dysmenorrhea. Majority of subjects experienced primary dysmenorrhea (77.5%) with mild degrees (53.7%). Most of the subjects classified as non-emotional eaters (56.3%), had a mild level of physical activity (58.7%), and the quality of their diet classified to need improvement (86.2%). There was no significant correlation between nutritional status, emotional eating, physical activity, and quality of diet with dysmenorrhea status (p>0.05), but there was a significant correlation with the family history (p=0.045). There was no significant correlation between family history, emotional eating, physical activity, and quality of diet with the degree of dysmenorrhea (p>0.05), but there was a significant correlation with nutritional status (p=0.029).
Keyword: dysmenorrhea, emotional eating, nutritional status, physical activity, quality of diet

Judul: Isolasi Konstruksi dan Rekombinasi Gen Pengkode Reverse Transcriptase asal Simian Retrovirus-2 (SRV-2)
Abstrak: The availability of reverse transcriptase enzym as a valuable component for Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique has been providing by foreign companies. Therefore, it needs more cost and time to provide it. This study is an early step to produce in house reverse transcriptase rekombinant enzym from SRV-2. The purpose of this study was isolated, constructed, and rekombined SRV-2 reverse transcriptase gene to entry clone and destination vector. Isolation was done by PCR amplification technique using three pairs of primers, which are RT I (3282-3683), RTII (3261-3900), and RTIII (3169-4133). Construction and recombination in this study used Gateway method from Invitrogen. SRV-2 reverse transcriptase gene was isolated in this study with A-549 cell inoculated SRV-2 as source of virus. This gene amplify by PCR technique and resulted specific band for RTI 401 bp, RTII 639 bp, and RTIII 964 bp. Analysis of pENTR/SD/TOPO and pDEST17 indicated SRV-2 reverse transcriptase gene code has already inserted to this plasmid. This result indicated SRV-2 reverse transcriptase gene have isolated, constructed, and recombined to entry clone and destination vector.
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