Judul: Inhibition of carmoisine and rhodamine toxic effects on rat’s spleen lymphocytes proliferation activity by Urtica dioica L. leaf extract
Abstrak: Urtica dioica L. has long been known as a herbal remedy and nutritious addition to the diet. It has been consumed for a long time as medicinal plants in many parts of the world. This study aimed to observe the effect of U. dioica L. leaf extracts in enhancing proliferation of rat’s spleen lymphocytes. Furthermore, this study also aimed to observe the capability of U. dioica L. leaf extracts in defending lymphocyte against toxic effects of carmoisine and rhodamine B . 30 Sprague Dawley rats were split into three groups, which are control, 0.1 g/kg BW/day dose, 1 g/kg BW/day dose group. The control group was given drinking water while the others were given U. dioica L. leaf crude extracts at their dose respectively. After 90 days of treatment, lymphocyte cells from the rat’s spleen then isolated and tested using MTT proliferation assay with addition of mitogen, and various concentration of carmoisine and rhodamine B. Stimulation index of spontaneous proliferation in 0.1 g/kg BW/day dose group was higher than the other groups. Mitogen-induced proliferation shows an increased proliferation activity in control and 1 g/kg BW/day dose group, while in 0.1 g/kg BW/day dose group a decreased proliferation activity was observed. U. dioica L. leaf crude extracts given at 1 g/kg BW/day shows that the rat’s lymphocyte proliferation activity at the presence of carmoisine was higher than control and 0.1 g/kg BW/day dose group. Likewise, at the presence of rhodamine B, the rat’s lymphocyte proliferation activity of 1 g/kg BW/day dose group was higher than control and 0.1 g/kg BW/day dose group. It is concluded that U. dioica L. leaf crude extract given at 1 g/kg BW/day could inhibit carmoisine and rhodamine B toxic effect on rat’s spleen lymphocytes proliferation activity.
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Judul: Aktivitas ekstrak etanol-air daun kari (Murraya koenigii) sebagai hepatoprotektor pada tikus putih galur Sprague Dawley
Abstrak: Curry leaves (Murray koenigii), a traditional Indian medicinal plant is one of the herbal plants that has been reported to have high in vitro antioxidant activity. This study was designed to investigate phytochemical compound and hepatoprotective activity of ethanol:water (1:1) extract of curry leaves (EWEC) on paracetamol toxic dose induced acute liver damage in Sprague Dawley rats in vivo. Hepatoprotection activity was measured by using biochemical parameters such as enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) serum level and to observe the histopathology study of liver. Twenty five rats were divided into 5 groups: normal group (N), negative control group/KN (paracetamol 500 mg/Kg BB), positive control group/KP (Curliv-plus® 42,86 mg/Kg BB), EWEC groups doses 200 mg/Kg BB and 300 mg/Kg BB. Curry leave was extracted with a solvent mixture of ethanol:water (1:1) which resulted about 19,2% rendement. Phytochemical test indicates that EWEC contains alkaloids, saponins, steroids, and tannins. Transaminase enzyme serum analysis shows that the induction of EWEC 300 and KP treatment gives a significant effect (p<0.01) on hepatoprotective compared to EWEC 200. However, liver histopathology shows that there is a necrosis hepatocytes on KN group, regeneration on KP group, vacuolisation on EWEC 200, and normal cells on EWEC 300. These results indicate that the antioxidant of EWEC is potential to protect liver cells.
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Judul: PEmetaan Kawasan Peka Bencana Tsunami di wilayah Pesisir propinsi Nusa Tenggara Timur
Abstrak: Kondisi geologi Indonesia yang merupakan pertemuan lempeng-lempeng tektonik menjadi kawasan Indonesia memiliki tingkat kerawanan yang tinggi terhadap bencana alam seperti gempa bumi, tsunami dan rawan letusan gunung api. Gempa bumi yang terjadi di dasar laut mengakibatkan pergolakan massa air yang dapat menghasilkan gelombang besar atau disebut tsunami.
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