Judul: Analisis desain fungsional, struktural dan kondisi iklim mikro pada lumbung padi tradisional (Leuit) masyarakat baduy luar di propinsi Banten
Abstrak: Masyarakat Baduy dikenal mandiri dalam pemenuhan kebutuhan padinya. Masyarakat ini memiliki suatu lumbung padi yang disebut Leuit. Bangunan Leuit hanya memiliki satu pintu kecil untuk memasukkan dan mengeluarkan padi. Padi di dalam Leuit biasanya sangat awet dan dapat disimpan hingga lima tahun. Pada penelitian ini dilakukan analisa terhadap iklim mikro, desain fimgsional, serta desain spkhxal Leuit. Penelitian dilaksanakan di Desa Kanekes, Kecamatan Leuwidamar, Kabupaten Lebak, Banten dan Laboratorium Lingkungan dan Bangunan Pertanian, Fakultas Teknologi Pertanian IPB pada bulan April 2008 hingga September 2008. Parameter iklim mikro yang diamati antara lain suhu, RH (kelembaban relatif), dan intensitas cahaya. Parameter desain bangunan yang diamati antara lain ukuran bangunan, bahan konstruksi, berat bagian-bagian bangunan, dan jenis sambungan. Parameter tambahan yang diamati adalah proses panen dan pascapanen yang dilakukan oleh masyarakat Baduy Luar.
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Judul: Persepsi Komunitas Adat Baduy Luar Terhadap Kebutuhan Keluarga Di Kabupaten Lebak Provinsi Banten
Abstrak: Masyarakat Baduy adalah salah satu komunitas etnik yang masih memegang tradisi dan cenderung tertutup, atau dalam istilah sekarang Komunitas Adat Terpencil (KAT). Pengertian KAT, adalah kelompok sosial budaya yang bersifat lokal dan terpencar serta kurang atau belum terlibat dalam jaringan dan pelayanan baik sosial, ekonomi maupun politik. Komunitas Adat Baduy Dalam masih memegang teguh adat dan tradisi leluhur, sedangkan Baduy Luar cenderung sudah menerima perubahan, serta nilai- nilai dari luar. Tujuan penelitian adalah: (1) Memperoleh gambaran persepsi KAT pada kebutuhan keluarga masyarakat Baduy Luar, (2) Mengkaji faktor-faktor yang mempengaruhi persepsi masyarakat Baduy Luar terhadap kebutuhan keluarganya; dan (3) Mendapatkan suatu model perubahan terencana, macam intervensi, dan hal-hal yang perlu di intervensi untuk memenuhi kebutuhan keluarga Komunitas Adat Baduy Luar. Penelitian ini dirancang dengan metode survei, dengan tujuan mendeskripsikan, mengeksplanasi (expalanatory), dan mengeksplorasi serta menjelaskan tujuan, termasuk menjelaskan pengaruh dan hubungan antar peubah lewat pengujian hipotesis
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Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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