Judul: Penggunaan Metode Bootstrap Untnk Menduga  Selang Kepercayaan Total Populasi Pada  Populasi Spasial Bergerombol
Abstrak: Metode penarikan contoh gerombol adaptif baik digunakan untuk menduga total populasi pada populasi spasial bergerombol. Dalam penarikan contoh gerombol adaptif ketika unit-unit dalam contoh awal memenuhi kriteria yang ditetapkan, maka unit tetangganya akan inasuk ke dalam contoh juga. Pendugaan tak bias total populasi pada penarikan contoh gerombol adaptif menggunakan pendekatan modifikasi Hansen-Hurwitz dan pendekatan modifikasi Horvitz-Thompson.
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Judul: Pengkajian Keakuratan TwoStep Cluster dalam Menentukan Banyaknya Gerombol Populasi
Abstrak: krataan tidak dapat menangani peubah (atribut) campuran kategorik dan numerik, serta penentuan banyaknya gerombol. Metode TwoStep Cluster (Chiu et al. 2001) dapat menangani peubah campuran kategorik dan numerik dan penentuan banyaknya gerombol secara objektif (otomatis). Penelitian ini mengevaluasi keakuratan TwoStep Cluster dalam menghasilkan banyaknya gerombol dan ukuran gerombol yang sama dengan populasi yang sebenarnya. Perbandingan struktur gerombol dalam populasi hipotetik dengan hasil penduga banyaknya gerombol yang dihasilkan dari algoritma TwoStep Cluster dapat diperoleh dengan studi simulasi. Algoritma TwoStep Cluster sangat akurat dalam menduga banyaknya gerombol dan sebaran ukuran gerombol sebenarnya jika semua peubah kontinu. Transformasi peubah kategorik ke dalam bentuk biner dan memperlakukannya sebagai peubah numerik meningkatkan keakuratan TwoStep Cluster dalam menduga banyaknya gerombol, bilamana peubah kriteria penggerombolan merupakan peubah campuran. Selain itu TwoStep Cluster juga sangat akurat dalam menghasilkan gerombol yang sama dengan populasi sebenarnya pada kasus-kasus data yang terpisah secara tegas, atau setidaknya terlihat adanya perbedaan penggerombolan pada data yang dianalisis.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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