Judul: Pemodelan Spasial Konservasi Lingkungan Dengan Pendekatan Sosial Ekonomi Masyarakat Di Kota Ambon
Abstrak: The increase of population may cause a domino effect in all aspects of life, especially in urban areas. The presence of vegetation cover will also threatened when the population continues to increase and subsequently may cause various effects of the erosion, flooding, landslides, and so on. This study, was performed to establish the Environmental Conservation Index (IKL) in Ambon to measure sustainability of the environment. The study was conducted from January to March 2012 in the Ambon City. Data collection was derived form face-to-face interviews with 440 respondents and secondary data compilation. The data analysis includes (a) weight determination; (b) score establishment; and (c) environmental conservation index development. The IKL models developed in this study is: IKL Urban = 0,127*Tl_skor + 0,095*Slop_skor + 0,175*Psm_skor + 0,238*Pdk_skor + 0206*Pddk_skor + 0,159*Pdptn_skor. The study found that 30.8 % of the Ambon City is categorized into conservative; 49.6% belong to less conservative categories, and into 19.7% extriemely conservative. Major proportion of the public community provides positive perseption to the importance of environment problem in Ambon City, where 77% of the activities involved in environment conservation. About 50% of the community expresses their satisfication the performance of the to government on management environmental problem.
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Judul: Analisis Keberelanjutan Lokal Kota Depok, Jawa Barat
Abstrak: Depok City is part of the Greater Jakarta Metropolitan Area which is experiencing urban expansion and high population migration pressure. However, within last decade there has been an imbalance development, where economic and social dimensions tend to be better than the performance of environmental one. This can be seen from the increase of economic and public service facilities, in contrast to a decrease in environmental quality. The performance of Depok City's sustainable development can be measured at a micro analysis (at the kelurahan level), namely by calculating the Local Sustainability Index (IKL). This research was carried out in Depok City from January 2021 to December 2021 by using several characteristics of Depok City villages in 2018, collected from Figures for the Depok City in 2018, as well as the Depok City administration map. The IKL was computed employing factor analysis (FA) on Statistica 7.0 and then the spatial distribution was mapped based on administrative region in ArcGIS. The Spatial Association of IKL was evaluated by using Global Moran Index and LISA (Local Indicator of Spatial Autocorrelation) with GeoDa, and the typology of the region was then grouped by employing cluster analysis on Statistica 7.0. The results showed that economic IKL was higher than social and environmental IKLs. The spatial pattern of social, economic, and environmental IKL as indicators of local sustainability was somewhat random. Global Moran Index for social, economic, and environmental dimensions were 0.209, 0.229, and 0.202, respectively, which indicate positive autocorrelations describing spatially clustered pattern. The cluster analysis resulted 63% of urban villages occupied Cluster 1 (having a very low IKL for social dimension value, economic and environmental dimensions are classified as moderate value), 24% of villages occupied Cluster 2 (having a high IKL economic dimension value, social and environmental dimensions are classified as low IKL value, and 13% of villages occupied Cluster 3 (having a high IKL social dimension values, economic and environmental dimensions are classified as moderate IKL value). Furthermore, the results of this research can be used by the government of Depok City as a recommendation in formulating policies, plans, and/or programs in an effort to actualize sustainable development in each kelurahan.
Keyword: cluster analysis, factor analysis, Global Moran Index, Local Sustainability Index, LISA

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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