Judul: Incidence and Severity of Corn Diseases in Cindaga Village, Kebasen District, Banyumas Regency, Central Java
Abstrak: Jagung (Zea mays L.) merupakan salah satu bahan makanan pokok di beberapa daerah di Indonesia. Penyakit jagung dapat menghambat pertumbuhan tanaman yang menyebabkan penurunan hasil. Penelitian ini bertujuan menginventarisasi, mengidentifikasi, serta mengukur tingkat insidensi dan keparahan penyakit pada tanaman jagung di Desa Cindaga, Kecamatan Kebasen, Kabupaten Banyumas, Jawa Tengah. Penelitian dilaksanakan dalam enam tahap, yaitu penentuan lokasi penelitian, penentuan tanaman contoh, identifikasi penyakit, pengamatan insidensi dan keparahan penyakit, wawancara petani, dan analisis data. Pengamatan penyakit dilakukan pada tiga lahan jagung dengan luas masing-masing 350 m2. Sebanyak 40 tanaman contoh setiap lahan diamati secara sistematik. Wawancara dilakukan untuk memperoleh informasi teknik budi daya petani. Berdasarkan gejala penyakit, tiga penyakit jagung yang berhasil diidentifikasi adalah bercak daun (Curvularia sp.), hawar daun (Helminthsporium sp.), dan karat daun (Puccinia sp.). Bercak daun merupakan penyakit terpenting dengan insidensi 100% dan keparahan penyakit 67-73%. Insidensi penyakit hawar daun dan karat daun berkisar 63-73% dan 35-58%, dengan keparahan penyakit 43-48% dan 5-46%. Kondisi cuaca serta teknik budi daya yang dilakukan petani mungkin menjadi faktor pendukung tingginya tingkat insidensi dan keparahan penyakit yang terjadi di lokasi pengamatan., Corn (Zea mays L.) is one of the staple foods at several regions in Indonesia. Diseases can inhibit plant growth leading to reduced yields. This study aims to inventory, identify, and measure the incidence and severity of disease in maize in Cindaga Village, Kebasen District, Banyumas Regency, Central Java. The research was carried out in six stages, namely determining the research location, determining sample plants, identifying disease, observing the incidence and severity of disease, interviewing farmers, and analyzing data. Disease observations were carried out on three corn fields covering each area of 350 m2. A total of 40 sample plants per field were systematically observed. Interviews were conducted to obtain information on farmers' cultivation techniques. Based on the symptoms of the disease, three diseases of maize identified were leaf spot (Curvularia sp.), leaf blight (Helminthosporium sp.), and leaf rust (Puccinia sp.). Leaf spot was the most important disease with 100% disease incidence and 67-73% disease severity values. The disease incidence of leaf blight and leaf rust ranged from 63-73% and 35-58%, with disease severity 43-48% and 25-46%, respectively. Weather conditions and cultivation techniques practiced by farmers might become contributing factors to the high incidence and severity of the diseases.
Keyword: cultivation, Curvularia sp., Helminthosporium sp., Puccinia sp

Judul: Pengelolaan penyakit hawar daun (Helminthosporium spp.) pada tanaman jagung (Zea mays) di Kabupaten Solok Selatan
Abstrak: Jagung merupakan komoditas penting di Kabupaten Solok Selatan. Hawar daun (Helminthosporium spp.) adalah salah satu penyakit penting yang menyerang tanaman jagung. Penelitian ini bertujuan menghitung insidensi dan keparahan penyakit, serta mengetahui tingkat pengetahuan, sikap, dan tindakan petani dalam pengelolaan penyakit hawar daun jagung di Kabupaten Solok Selatan. Pengamatan insidensi dan keparahan penyakit dilakukan di Kecamatan Sangir dan Kecamatan Pauh Duo, masing-masing kecamatan dipilih empat lahan. Pada setiap lahan diamati 60 tanaman contoh yang dipilih secara diagonal. Wawancara dilakukan untuk mengetahui teknik budi daya, pengetahuan, sikap dan tindakan petani tentang pengelolaan penyakit hawar daun jagung. Data insidensi dan keparahan penyakit ditabulasi menggunakan Microsoft Excel 2013 dan dianalisis secara deskriptif. Dua penyebab penyakit hawar daun jagung yang ditemukan adalah Helminthosporium turcicum dan H. maydis. Insidensi penyakit mencapai 100% dengan keparahan penyakit paling tinggi sebesar 43,33%. Dalam pengelolaan penyakit hawar daun jagung, tingkat pengetahuan dan tindakan petani responden tergolong masih kurang, sikap cukup baik., Corn is an important commodity in South Solok Regency. Leaf blight (Helminthosporium spp.) is one of the important diseases infecting corn plants. This study aims to calculate the development of the disease incidence and severity, as well as to determine the level of knowledge, attitudes, and actions of farmers in managing corn leaf blight in South Solok Regency. Observations of disease incidence and severity were carried out in Sangir and Pauh Duo sub-districts, each sub-district was selected four corn fields. In each field, 60 sample plants were observed which were selected diagonally. Farmer interviews were conducted to determine the cultivation techniques, knowledge, attitudes, and actions of farmers in managing the corn leaf blight. Data on the disease incidence and severity were tabulated using Microsoft Excel 2013 and analyzed descriptively. Base on disease symptom, two pathogens of corn leaf blight identified were Helminthosporium turcicum and H. maydis. The incidence of disease reached 100% with the highest disease severity of 43,33%. In the management of corn leaf blight, the level of knowledge and action respondent farmers are still lacking, while attitudes level is quite good.
Keyword: farmer behavior, plant disease, severity

Judul: Pemurnian Rekombinan Eritropoietin Manusia (rhEPO) Menggunakan Kromatografi Afinitas Ni-NTA dan Co-Talon
Abstrak: Suitable and efficient techniques for purification of recombinant human erythropoietin (rhEPO) are required to obtain pure rhEPO which can be continued to preclinical trials step. One of these techniques is immobilized metal affinity chromatography (IMAC), which has advantages such as high selectivity, easy to elute, and simple procedure for column regeneration. At least, there are 2 kinds of commercial IMAC resin using nitriloacetic acid (NTA) matrix as chelating agent, namely nickel-based (Ni-NTA) and cobalt-based IMAC resin (Co-NTA), the latter is well known as Talon. This research studied the influence of metal ions Ni2+ and Co2+ in rhEPO purification. The results indicated that Ni- NTA had higher affinity in binding rhEPO protein compared with Co-Talon. Average total protein by Ni-NTA was 226.72 μg/mL and Co-Talon was 60.52 μg/mL, but the protein purity with Co-Talon was better than Ni-NTA. Base on protein yield that can be separated, Ni-NTA is more suitable to obtain high yield of rhEPO protein.
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