Judul: Persepsi pelajar SMA umum di Kotamadya Yogyakarta tentang lingkungn hijau di perkotaan
Abstrak: Masalah lingkungan hidup semakin rumit dengan adanya pertambahan penduduk serta kemajuan ilmu dan teknologi. Salah satu fakta yang memperlihatkan terganggunya ekosistem dan menurunnya kualitas lingkungan adalah terdesaknya ruang terbuka hijau akibat adanya pembangunan di perkotaan. Sumber utama masalah lingkungan adalah manusia. Upaya peningkatan kesadaran masyarakat tentang pentingnya lingkungan hijau sebagai bagian dari lingkungan hidup, sudah sejak dulu dilaksanakan. Tetapi dirasakan bahwa upaya pembinaan kesadaran berlingkungan sehat untuk masa depan merupakan usaha yang sangat sulit. Menghadapi kenyataan diatas, pelajar sebagai bagian dari masyarakat yang memiliki potensi terbesar dalam pembangunan. di masa depan, jelas menjadi titik perhatian utama. Terlebih lagi dirasakan, dukungan pelajar dalam kegiatan di bidang perbaikan dan pelestarian lingkungan tidak menunjukkan peran aktifnya. Hal ini dapat terjadi karena kurangnya persepsi mereka terhadap lingkungannya. Seberapa jauh pengertian dan pemahaman pelajar tentang lingkungan hingga terbentuk suatu persepsi, sampai sekarang belum ada penelitian yang menguraikan masalah tersebut.
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Judul: Persepsi guru dalam penerapan pendidikan lingkungan hidup di sekolah dasar sekitar hutan kawasan Gunung Salak Endah Kabupaten Bogor
Abstrak: This research aimed at identifying teacher’s perception of the environment, teacher’s perception of Environmental Education (EE) implementation, and the influencing factors. Data were collected through structured questionnaire, guided interview, field observation, as well as archives and secondary documents. Factor analysis, Spearman correlation, Kruskal-Wallis test, and Mann-Whitney test were used to analyse the data. The results showed that, first, according to the result of the Draw-An-Environment-Test (DAET), teachers from forest margin area have limited environmental perception due to lack of capacity in expressing their thoughts in drawings and writings. However, since writing and drawing are not yet internalized as an important behavior in education, the DAET instrument should be critically improved or revised to make it fits for developing countries such as Indonesia. Second, according to the result of Factor Analysis, the perception of teachers toward EE is constructed from three major factors, i.e. the EE teaching effectiveness, its benefits, and outcome expectancy. The first factor – the EE teaching effectiveness – is formed by four variables i.e. perceived competence, self-efficacy, pressure/tension and perceived choice. Further analysis shows that the teacher often views him or herself as having low competence and self-efficacy due to the EE course experience and his/her educational level. The second factor resulted from Factor Analysis – the EE benefits – is formed by three variables, i.e. interest/enjoyment, efforts, and usefulness of the EE course. The EE benefits itself, according to Kruskal-Wallis test, are determine by EE training course received, level of grade taught and the nature-based organizational experienced by the teachers. Third, in order to reach effective EE teaching, the capacity, motivation, perceived competence and self-efficacy of the teachers should be strengthen. This includes but not limited to the mastery of environment-related subjects particularly forest conservation topics, strengthen skills for outdoor teaching and increased compassion on the environmental issues and/or forest related problems.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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