Judul: The Development of Brazilin Isolation Method from Sappan Wood (Caesalpinia sappan)
Abstrak: The objectives of this research was to develop efficient and effective the brazilin isolation method from sappan wood (Caesalpinia sappan) to obtain high yield and high purity. Brazilin is a compound with a lot of activities, but it is difficult to find this compound as standard. The development method was done by modifying the extraction method and optimizing the mobile and stationary phase in the separation process. Modification of the extraction was done by removing non-polar compounds with n-hexane. The yield of methanol fraction was 8.64 % (w/w) while the yield of n-hexane fraction was 0.93 %. The optimum mobile and stationary phase were chloroform: methanol (5:1) and silica gel for thin layer chromatography (TLC) and column chromatography. Methanol extract was column chromatographed, eluted with chloroform;methanol (5:1) to get the early fractions (Rf 0.89 in TLC condition). The fractions were further purified by preparative TLC. The results were analyzed by high performance liquid chromatography (HPLC) and characterized by UV-Vis and FTIR spectrometry. The yield of brazilin obtained 21.43 % (w/w) Rf value on TLC was 0.54 and the HPLC retention time was 15.695 minutes.
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Judul: Optimization High Performance Liquid Chromatographic Fingerprint of sappan wood (Caesalpinia sappan)
Abstrak: Mixture design has been applied for optimization of Caesalpinia sappan chromatographic fingerprint. The design applied for unreplicated and simultaneous optimization of HPLC mobile phase mixture. Ethanol extracts with highest antioxidant activity resulted from sonication extraction method was chosen for HPLC analysis . The reversed phase chromatographic mobile phase in simplex centroid design consist of varying proportion of methanol 50 %, methanol: water: TFA (25: 75: 0.025 v/v), and methanol: water: TFA (15: 85: 0.035 v/v). Ethanol 70 % extract of sappan analyzed with ten mobile phase and monitored at 254 and 280 nm. Correlation between HPLC mobile phase and a number of peak analyzed statistically by SAS 9.2. The root mean square errror of calibration (RSMEC) and root mean square errror of calibration (RSMEP) at 254 and 280 nm, were 8.3006 and 8.29659, 3.88730 and 4.85193 respectively. Optimum condition obbtained when ethanol 70 % extract eluted by methanol: water: TFA (15: 85: 0.035 v/v), with the 15 number of peaks.
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Judul: Infeksi Virus Parainfluenza Pada Anjing
Abstrak: Virus parainfluenza anjing termasuk famili Paramyxoviridae, berbentuk pleomorf dengan ukuran bervariasi (bergaris tengah antara 100-500 nm), berpembungkus peka ether, berisi genom RNA dan secara serologis mempunyai kesamaan dengan virus SV-5 kera; dikenak sebagai salah satu kausa penting dari sindrom "kennel cough". Infeksinya dapat mengenai anjing semua umur yang belum pernah terinfeksi atau mendapatkan vaksinasi mengakibatkan gejala klinis ringan yang terbatas pada traktus respirasi atu infeksi yang secara klinis tidak memperlihatkan gejala nyata. Gejala klinis yang nampak setelah masa inkubasi (1-5 hari) terlewati berupa batuk yang tidak berat selama beberapa hari, berbagai tingkat tonsilitis dan pharyngitis dan kadang-kadang ditemukan kenaikan temperatur rektal antara 1-2 derajat C dari temperatur normal rat-rata. Anjing terinfeksi mengandung virus pada traktus respirasi bagian atas dan bawah serta limfonoduli sampai hari ke 9 setelah infeksi. Penyebaran virus antar anjing terjadi secara aerogen.
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