Judul: Pengaruh Penambahan Asam Amino dalam Medium Kultur Bebas Serum terhadap Perkembangan Preimplantasi Embrio Mencit In Vitro
Abstrak: Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan asam amino esensial minimum (MEM) dengan clan tanpa asam amino non esensial glutamin ke dalam medium kultur MI6 bebas serum terhadap perkembangan embrio mencit in vitro. Pada penelitian ini digunakan medium MI6 modifikasi dengan penambahan 1.0 mg polivinyl alkohol ml" . Sehagai perlak~ian pada medium dasar dilakukan penambahan : (1) M16 + 0.3% BSA, (2) MI6 + 2% vlv MEM dan (3) MI6 + 2% vlv MEM + 0.1 mmolglutamin. Embrio yang dikultur dikoleksi pada 3 tahapan perkembangan yaitu: sigot (h-1), morula (h-3) dan morula kornpak (h-4). Parameter yang diamati adalah jumlah dan persentase embrio yang berkembang mencapai tahap 2 sel, 4 sel, 8 sel, morula kompak, blastosis, blastosis ekspan dan hatching.
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Judul: Pengaruh fosfat, glukosa dan kombinasinya dalam medium kultur in vitro terhadap perkembangan embrio mencit
Abstrak: Pada penelitian ini digunakan medium _Ml6 modifikasi yaitu medium Ml6 tanpa fosfat dan atau glukosa. Konsentrasi fosfat yang ditambahkan. dalam medium kultur masing-masing adalah 0,5 mmol/liter, 1,0 mmol/liter, 1,5 mmol/liter dan kontrol. Konsentrasi glukosa yang ditambahkan dalam medium kultur adalah 2,5 mmol/liter, 5,0 mmol/liter, 7,5 mmol/liter dan kontrol. Parameter yang diukur adalah jumlah dan persentase sigot mencit yang berkembang mencapai embrio tahap 2 sel, 4 sel, 8 sel, morula dan blastosis. Hasil penelitian menunjukkan bahwa konsentrasi fosfat 1, 0 mmol/liter dapat mendukung perkembangan embrio mencapai tahap blastosis. Pada tahap 2 sel konsentrasi fosfat 0,5 mmol/liter lebih baik mendukung perkembangan embrio, karena embrio yang berkembang bisa mencapai 64,6%, walaupun setelah dilakukan uji lanjut tidak berbeda nyata dengan konsentrasi fosfat 1,0 mmol/liter. Pada tahap 4 sel . kebutuhan fosfat cenderung meningkat dalam batas batas tertentu. Hal ini tampak pada konsentrasi fosfot 1, 0 mmol/liter jumlah embrio yang berkembang lebih tinggi dibandingkan dengan konsentrasi yang lain yaitu 5 I, 6%. Kemudian pada tahap 8 sel, morula dan blastosis jumlah embrio yang berkembang menurun, namun demikian konsentrasi fosfat I, 0 mmol/liter masih lebih baik dibandingkan dengan konsentrasi yang lain karena embrio yang berkembang mencapai 25%, 18,8% dan 9,4%. dst ...
Keyword: Kultur in vitro

Judul: Characteristic of Overshooting Top (OT) Based on  Himawari  8  Satellite  as  a  Convective  Cloud  Feature  that  Generates  Extreme Weather in the Java Island Region
Abstrak: Overshooting Top (OT) is a feature at the top of a convective cloud that is associated with extreme weather. Research on OT in tropical Indonesia is still very limited. The existence of geostationary satellites with wide area coverage and fast frequency observations is useful for observing the fast dynamics of the OT life cycle. Therefore, this study intends to analyze the relationship between OT and extreme weather, updraft and thermodynamic conditions of OT when extreme weather occurs, the best objective method for detecting OT in the day time and night time, and the spatiotemporal distribution of OT in the tropical region of Java Island during the 2018-2021 period. Based on visual analysis using visible channel and Himawari 8 sandwich products, 87% of extreme weather events in Java were accompanied by the appearance of OT. It indicates that the presence of the OT pattern is a convection cloud pattern or feature that is closely related to extreme weather events for the Java Island region. The existence of a time lag between the appearance of OT and extreme weather events on Java Island indicates that the OT of convection cloud can be used as a precursor or nowcasting for the appearance of extreme weather. Most of the convective cloud OT features during extreme weather events on Java Island have altitude that were only able to exceed the altitude of the equilibrium level and few were able to exceed the tropopause level of 9 km – 16 km. The difference in atmospheric conditions during extreme weather events on Java Island, which is in the tropics and subtropics, is a strong and unstable convective energy condition characterized by a larger CAPE, as well as unstable KI, LI, SI and TTI values. It is offset by kinematic factors in the form of a weak vertical wind shear (bulk wind shear 0-6 km) and a higher freezing point height. The difference between extreme weather events in the Java Island region and other tropical regions compared to subtropical regions is the strong updraft speed of convection clouds. The IRW Texture COMB algorithm based on the IRW-Texture Gradient modification and the COMB algorithm shows a significant increase in quality for detecting OT and non-OT pixels in Java. Rainy days with rainfall > 50 mm/day are very common in areas with an altitude of > 500 m. On the other hand, the level of convection activity which is indicated by the presence of OT and lightning is significantly reduced in this area. OT of convective clouds appear more frequently during the DJF season and less frequently during the JJA season. OT often appears during the day time and rarely at night time. Most OTs appear in the central part, away from the south and north coasts of Java Island during the day, while OTs that appear near the south and north coasts of Java Island can appear in the morning or afternoon.
Keyword: convective clouds, extreme weather, Overshooting Tops, Himawari 8 Satellite