Judul: in vitro callus and shoot induction of “mahkota dewa” by manipulation of plant growth regulators and explant
Abstrak: The objective of this research was to study the effects of auxin and cytokinin in in vitro callus and shoot induction of “mahkota dewa” (Phaleria macrocarpa). Three experiments used Complete Randomized Design for shoot induction (experiment I) and callus induction (experiment II and III). Shoot induction experiment consisted of 16 combinations of BA (0.0, 2.5, 5.0, and 7.5 mg/l) and NAA (0.0, 0.5, 1.0, and 1.5 mg/l) using MS basal medium and seed as explant. The callus induction experiment consisted of MS basal medium with nine combinations of 2,4-D (2.5, 5.0, and 10.0 mg/l) and BA (0.0, 0.5, and 1.0 mg/l) and six combinations of NAA (20.0 and 40.0 mg/l) and BA (0.0, 0.5, and 1.0 mg/l). Experiment II and III using leaf and seed as explants in separated experiment. within MS medium. The result of experiment I showed that shoots can be produced in MS basal medium with combinations BA and NAA. The best combination of auxin and cytokinin for shoot induction was BA 2.5 mg/l. For callus induction using leaf explants was 2,4-D 2.5 mg/l + BA 0.5 mg/l and 20.0 mg/l NAA without BA, and for seed explants was 2,4 -D 10.0 mg/l + BA 5.0 mg/l and NAA 20.0 mg/l + BA 0.5 mg/l but it is not significantly with NAA 20.0 mg/l + BA 1.0 mg/l.
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Judul: In Vitro Shoot Induction of Kaempferia parviflora Wall. Ex. Baker
Abstrak: This research aimed to find the best MS salt concentration and cytokinin (BAP) concentration for K. parviflora rapid multiplication by using in vitro buds as explants. The experiment was done at Plant Tissue Culture Laboratory of Agronomy and Horticulture Department, Faculty of Agriculture, Bogor Agricultural University from January to November 2011. This research began by sterilizing shoots of K. parviflora rhizomes received from PT. Ogawa Indonesia to produce axenic culture. After about 8 weeks of growth, young shoots began to sprout. The young shoots of K. parviflora axenic culture were then cut and cultured individually on the treatment medium, which consist of two different MS salt concentrations (MS and ½ MS) and five different 6-benzylaminopurine (BAP) concentrations (0, 1.25, 2.5, 3.75, and 5 ppm) respectively. Explants started to grow after 2 weeks of culture. Both MS salt concentration and BAP concentration treatments did not give a significant effect on the first shoot induction time, but ½ MS medium supplemented with 5 ppm BAP could induce first shoot faster than other treatments, which is within an average of 2.5 weeks after cultured (WAC). At early observation (2 to 4 WAC), medium enriched with 3.75 ppm BAP induced more shoot than the other treatments, but at the end, control medium (MS0) induced more shoot than other treatments. Starting from 2 WAC, the explants cultured on 0 ppm BAP medium grow more rapidly than the other treatments, but after 10 WAC, they grow slower. All treatments have the same trend of leaf growth. At 2 to 8 WAC, the number of leaves is rising rapidly, but at the 9 WAC, it began to slow down.
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Judul: Konstruksi Persamaan Friedmann Alam Semesta Friedmann-Lemaitre-Robertson-Walker Termodifikasi Materi Gelap Superfluida
Abstrak: Kosmologi merupakan salah satu objek kajian dari teori relativitas umum. Salah satu model dalam kosmologi adalah model kosmologi Friedmann. Model kosmologi Friedmann dibangun berdasarkan asumsi bahwa materi dan energi terdistribusi secara homogen dan isotropik di alam semesta. Teori relativitas umum dapat dimodifikasi melalui pendekatan teori materi gelap superfluida (MGS). Persamaan medan Einstein untuk teori MGS dapat dirumuskan melalui prinsip aksi dengan memodifikasi Lagrangian pada aksi teori MGS. Selanjutnya dengan memilih metrik Friedmann-Lemaitre-Robertson-Walker (FLRW) datar dengan indeks kurvatur sama dengan nol, dapat dibangun suatu bentuk persamaan Friedmann yang dimodifikasi teori MGS.
Keyword: kosmologi, teori materi gelap superfluida, persamaan medan Einstein, metrik FLRW, persamaan Friedmann.