Judul: Identification of Staphylococcus aureus from Vagina of Limousine Cattle with Repeat Breeding in BPTU-HPT Padang Mengatas
Abstrak: Repeat breeding (RB) merupakan satu diantara kasus gangguan reproduksi yang umum terjadi pada sapi yang dapat menyebabkan penurunan fertilitas induk dan angka kebuntingan. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri Staphylococcus aureus yang diduga menjadi satu diantara penyebab kasus repeat breeding pada sapi Limousin di BPTU-HPT Padang Mengatas. Sampel yang digunakan sebanyak lima ekor sapi Limousin yang mengalami RB. Spesimen berasal dari cairan vagina sapi yang mengalami RB. Metode uji yang digunakan untuk identifikasi bakteri adalah kultur, pewarnaan Gram dan uji biokimia. Hasil uji identifikasi menunjukkan bahwa pada lima sampel yang diuji tidak ditemukan bakteri Staphylococcus aureus. Namun, ditemukan bakteri lain diantaranya Micrococcus sp., Bacillus sp., Escherichia sp., Achromobacter sp., dan Alcaligenes sp. Kata Kunci: gangguan reproduksi, repeat breeding, sapi Limousin, Staphylococcus aureus, Repeat breeding (RB) is one of the common cases of reproductive disorders in cattle that could lead to decrease of calf birth and conception rate. The objectives of this study was to isolate and identify the Staphylococcus aureus which was suspected as one of the causes of repeat breeding cases on Limousine cattle in BPTU-HPT Padang Mengatas. The samples used were five Limousine cattle which suffer from RB. The specimens came from vaginal discharge of the cattle which suffer from RB. The test method used to identify bacteria are culture, Gram stain and biochemical test. The results of the identification test showed that there is no Staphylococcus aureus bacteria found over the five samples tested. However, there were other bacteria found including Micrococcus sp., Bacillus sp., Escherichia sp., Achromobacter sp., and Alcaligenes sp. Keywords : Limousine cattle, repeat breeding, reproductive disorder, Staphylococcus aureus
Keyword: repeat breeding, Staphylococcus aureus, Gangguan reproduksi, sapi Limousin

Judul: Isolation and Antimicrobial Resistance  Detection of Staphylococcus aureus from Pondok Ranggon Dairy Farm, East  Jakarta
Abstrak: Infeksi yang disebabkan oleh Staphylococcus aureus dapat ditularkan dari seekor sapi ke sapi lain dan dari puting yang terinfeksi ke puting yang normal atau sehat melalui tangan pemerah dan lingkungan. Keberadaan S. aureus berkaitan dengan manajemen higiene dan sanitasi yang buruk. Penggunaan antimikroba yang tidak tepat berpotensi meningkatkan resistensi bakteri terhadap antibiotik yang menjadi ancaman serius bagi kesehatan hewan dan masyarakat. Penelitian ini bertujuan mengidentifikasi bakteri S. aureus dari tangan pemerah dan permukaan puting sapi serta mendeteksi resistensi bakteri S. aureus terhadap beberapa antibiotik. Pengujian diawali dengan isolasi dan identifikasi bakteri dan dilanjutkan dengan pengujian resistensi bakteri terhadap antibiotik menggunakan metode Kirby-Baurer disk diffusion. Antibiotik yang digunakan terdiri atas sepuluh jenis yaitu penisilin,amoksisilin-klavulanat, streptomisin, gentamisin, eritromisin, tetrasiklin, siprofloksasin, asam nalidiksat, trimetropim sulfametoksazol, kloramfenikol. Keberadaan S. aureus yang diisolasi dari tangan pemerah maupun dari puting sapi di Peternakan Sapi Perah Pondok Ranggon, Jakarta Timur masih cukup tinggi. Berdasarkan pengujian isolasi S. aureus dari sampel tangan pemerah diperoleh sebanyak 77,3% dan dari puting sapi sebanyak 72,9%. Hasil penelitian menunjukkan 44,2% isolat S. aureus yang diisolasi dari swab tangan pemerah dan puting sapi telah mengalami multi-drug-resistant (MDR). Kejadian resistensi pada S. aureus perlu menjadi perhatian karena berpotensi sebagai ancaman kesehatan pada hewan maupun manusia karena memiliki potensi zoonosis, Infections caused by Staphylococcus aureus can be transmitted from one cow to another and from the infected cow’s teat to the normal and healthy teat by milker’s hand and the environment. The presence of S. aureus is associated with poor hygiene and sanitation practices. Improper use of antimicrobials has the potential to increase bacterial resistance to antibiotics which are a serious threat to animal health and society. This study aims to identify S. aureus from milker’s hand and the surface of cow’s teat and to detect the resistance of S. aureus to several antibiotics. Testing begins with the isolation and identification of bacteria and continues with testing for bacterial resistance to antibiotics using the Kirby Baurer method. The antibiotics used consisted of 10 types, penicillin, amoxicillin clavulanate, streptomycin, gentamicin, erythromycin, tetracycline, ciprofloxacin, nalidixic acid, trimethoprim-sulfamethoxazole, and chloramphenicol. The presence of S. aureus isolated from the milker’s hand or the cow’s teat at Pondok Ranggon Dairy Farm, East Jakarta is still quite high. Based on the isolation test of S. aureus from milker’s hand sample, it was 77,3% and 72,9% from the cow's teat, with 44,2% of them showed multi-drug resistance (MDR). The incidence of S. aureus resistance needs to be a concern because it has zoonotic potential to human and animals.
Keyword: puting sapi, resistensi antibiotik, Staphylococcus aureus, tangan  pemerah

Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4