Judul: Gaya Pengasuhan, Pola Komunikasi, Kelekatan, dan Hubungannya dengan Kepuasan Remaja
Abstrak: This research aimed to analyze parenting style, communication pattern (communication type and time allocation), attachment, and correlation with adolescence’s satisfaction. The location of this research is in SMP Negeri 1 Dramaga and adolescence’s house arround Dramaga Subdistrict, Bogor District. This research used cross sectional design, involved 30 male adolescences, 30 female adolescences and 60 mothers as participant. The samples choosen by stratified random sampling. Data was collected through interview using structured questionnaire. Data was analyzed by descriptive, independent sample t-test, and Pearson corellation analysis. The result of this research shows that mostly adolescence have democratic parenting style perception. Almost two-thirds of male adolescence perceive communication type structural traditionalism, more than twothirds female adolescence and mother perceive family communication types expresiveness. More than half adolescence and mother have 5-15 minutes each day for communication each other. The categorize of attachment is high security for all adolescence and more than three-fourth adolescence’s satisfaction is average. There was positive significant correlation between parenting style with communication type, communication type with attachment, and attachment with adolescence’s satisfaction
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Judul: ting Style, Father-Adolescence Interaction, Attachment, and Father’s Satisfaction.
Abstrak: Penelitian ini bertujuan untuk mengetahui hubungan antara gaya pengasuhan, interaksi ayah-remaja, kelekatan, dan kepuasan ayah. Desain yang digunakan dalam penelitian ini adalah cross sectional study, melibatkan 60 ayah, terdiri atas 30 ayah dengan remaja laki-laki dan 30 ayah dengan remaja perempuan yang berusia 12-14 tahun. Tempat penelitian ini adalah di Kelurahan Panaragan, Kecamatan Bogor Tengah. Data dianalisis secara deskriptif, uji beda t-test, dan korelasi Pearson. Hasil menunjukkan bahwa hampir seluruh remaja mempersepsikan diasuh dengan menggunakan gaya pengasuhan demokratis. Pola komunikasi yang digunakan dalam keluarga sebagian besar adalah conversation-orientation dengan tipe komunikasi consensual. Rata-rata waktu dalam sehari yang diberikan oleh ayah untuk berinteraksi dengan remajanya adalah 0,47 jam untuk remaja laki-laki dan 0,64 jam untuk remaja perempuan. Hubungan yang postif signifikan terdapat antara gaya pengasuhan demokratis dengan pola komunikasi conversation-orientation. Pola komunikasi conversation-orientation yang diterapkan oleh ayah berhubungan positif dengan dimensi kepercayaan pada kelekatan. Pola komunikasi conformity-orientation yang dilakukan oleh ayah berhubungan negatif signifikan dengan tingkat kepuasan
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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