Judul: Identifikasi Karakter Aromatik secara Molekuler dan Organoleptik pada Galur Padi BC5F2 Hasil Persilangan Ciherang dan Pandanwangi
Abstrak: Aroma in rice has become an important consideration in increasing consumer appetite. Genetic mapping of molecular marker related to the responsible for fragrance in rice was previouslyreported and it was closely linked with microsatellite marker RM223. This study aimed to identify the aromatic character of the gene badh2 in BC5F2 populations derived from crosses between Ciherang and Pandanwangi, both with molecular marker RM223 and organoleptic analysis using KOH 1.7% solution. Based on the foreground selection analysis of the total 272 individual tested lines, 105 lines were found to be homozygous for Pandanwangi (160 bp), 46 lines were homozygous for Ciherang (140 bp) and 121 lines were heterozygous (140 bp and 160 bp). Evaluation of aroma with KOH in selected BC5F2 lines showed variation in quality value, such as slight, moderate, and strong aroma. Thus, through these two selection methods, we were able to select several BC5F2 lines carrying aromatic trait introgressed from Pandanwangi at target region.
Keyword: aroma, badh2, Ciherang, Pandanwangi, RM223

Judul: Identifikasi Gen Aroma Pada BC5F1 Ciherang-Pandan Wangi dan BC4F1 Ciherang-Mentik Wangi
Abstrak: The demand of high quality rice becomes increasingly in Asian market and around the world. The increase in productivity and quality of Indonesian agriculture has continued, such as improving new aromatic rice variety to have good characteristics (resistant to pest, ) which is like non-aromatic rice. The aim of this research was to develop new varieties of non transgenic aromatic rice by induction of aroma gene (mutated badh2) from aromatic varieties (Pandan Wangi or Mentik Wangi) to non aromatic varieties rice (Ciherang). Rice BC5F1 CP (Ciherang/Pandan Wangi) and BC4F1 CM (Ciherang/Mentik Wangi) that used in this research were the result from previous research with site-directed crossing method. Selection of plant was done by PCR with RM223 marker for CP and Bradbury marker for CM. Visualization of DNA sample by PCR was done by using gel agarose 3% for CP and 2% for CM. DNA amplification result of BC5F1 CP with RM223 marker can distinguish the band pattern of aromatic and non aromatic with band size 140 bp and 160 bp. In addition, DNA amplification result of BC4F1 CM with Bradbury marker produced DNA band size 580 bp, 355 bp and 257 bp. The succeed in introgression of aroma gene from Pandan Wangi or Mentik Wangi to Ciherang was showed by heterozygote band.
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Judul: Hydroxyapatite-Chitosan Composite Coating on Stainless Steel 316 to Improve Corrosion Resistance
Abstrak: Metals that were used in bone implantation should have good corrosion resistance that were not easily corroded when implanted into the body. Metal corrosion resistance of stainless steel 316 can be enhanced through the metal coating by hydroxyapatite-chitosan composite with composition ratio of hydroxyapatite (gram) and chitosan 3% (mL) 0.2:0, 0.2:0.1, 0.2:0.5, 0.2:1.0, and 0.2:1.5. Besides improving the corrosion resistance of metal, the presence of chitosan also maintain the position of hydroxyapatite on metal surfaces. Corrosion resistance increases with increasing amount of chitosan added and the best composition of hydroxyapatite-chitosan composite was 0.2:1.0.
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