Judul: Ketidaksesuaian Mengajar Dilihat Dari Unit Mata Pelajaran
Abstrak: Salah satu masalah yang dihadapi dalam dunia pendidikan adalah masalah mutu, bahwa kualifikasi guru yang diminta oleh SMU tidak sesuai dengan lulusan guru yang telah tersedia dilihat dari kebutuhan per bidang studinya. Dalam penelitian ini dibuat kriteria kesesuaian rnengajar dilihat dari unit mata pelajaran untuk wilayah Bogar, Tangerang, Bekasi, Depok dan wilayah gabungan Botabekdek. Adapun kriteria kesesuaian yang dibuat terdiri dari dua kriteria, dengan maksud untuk melihat pembuatan kriteria yang berbeda tersebut terhadap jumlah kesesuaian mengajar yang ada. Di wilayah Botabekdek pada kriteria I terdapat 711 (52%) guru yang telah sesuai dalam mengajar mata pelajaran sedangkan pada kriteria 2 terdapat 1167 (85%) guru yang telah sesuai dalam mengajar.
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Judul: Analisis Ketaksesuaian Latar Belakang Pendidikan Guru dengan Mata Pelajaran SMA (Studi Kasus pada SMA Provinsi Jawa Barat)
Abstrak: Teachers are professional educators with the primary task of educating, teaching, guiding, directing, training, and evaluating students. There have been many efforts to increase the ability of teachers, one of which is to increase the academics qualification through minimum qualification of education that is S1/D4. Besides the issue of teachers qualification, there's the issue of mismatch between teachers education background with the subjects that they teachs. Mismatch analysis was used in this study to examine the mismatch issue in West Java Province. The selection of this region was based on the highest percentage of high school teachers in Indonesia, West Java has the highest percentage of 10.56 % teachers in Indonesia. Incomplete data is the data that is not filled by teachers. There are seven districts and cities having incomplete data which is excluded in this study. Mismatch index values are divided into four categories, which are Very Low, Low, Medium, and High. For all subjects that were taught in high school, regions which had high mismatch index include Sukabumi, Ciamis, Kuningan, Indramayu, Purwakarta, and Banjar. Whereas for each subject that had a high mismatch index include Anthropology, Regional Languages, Local Content, Sociology, Communication and Information Engineering. Teachers who teach in these five subjects require more attention, because the mismatch index is more higher than the other subjects
Keyword: Senior High School, West Java, Mismatch Analysis

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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