Judul: Hubungan Antara Jenis Informasi Seks, Jenis Saluran  Komunikasi dan Persepsi Tentang Perilaku Seksual Pra Nikah  pada Remaja ( Kasus Sebuah SMU di Bogor)
Abstrak: Perkembangan teknologi komunikasi dan iklim kebebasan pers telah memunculkan banyak media (media cetak, audio, visual dan audiovisual) yang menyampaikan berbagai jenis informasi seks. Di sisi lain, keingintahuan remaja terhadap informasi seks cukup tinggi serta meningkatnya permasalahan hubungan seksual pra nikah pada remaja.
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Judul: Hubungan komunikasi interpersonal dan pemanfaatan internet dengan pengetahuan seks remaja kasus: siswa Sekolah Menengah Atas Plus Bina Bangsa Sejahtera (BBS), Kecamatan Bogor Barat, Kota Bogor
Abstrak: Remaja dalam menjalani hidup tidak pernah lepas dari perubahan. Perubahan-perubahan akan selalu ada dan ditemukan dalam diri setiap remaja, dimana remaja harus bisa melalui masa ini sebagai bagian dari rentang kehidupan. Remaja merupakan individu yang sedang mengalami masa-masa transisi (peralihan), dari masa kanak-kanak menuju masa kedewasaan. Masa ini merupakan masa-masa yang bermasalah, masa yang penuh gejolak, masa yang mengalami konflik peran dan masa yang harus dihadapi oleh setiap remaja. Penelitian ini dilakukan dengan tujuan untuk : 1) Mengidentifikasi hubungan antara karakteristik individu dengan pengetahuan seks remaja; 2) Menganalisis hubungan antara komunikasi interpersonal dengan pengetahuan seks remaja; dan 3) Menganalisis hubungan antara akses internet dengan pengetahuan seks remaja. Penelitian ini dilakukan di Sekolah Menengah Atas Plus Bina Bangsa Sejahtera (SMA Plus BBS), Kecamatan Bogor Barat, Kota Bogor. Responden penelitian ini adalah Siswa Kelas XI (setara dengan kelas 2 SMU) yang berusia antara 16-17 tahun. Penelitian dilakukan selama dua bulan yaitu dari bulan Agustus sampai dengan September 2006.
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Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable