Judul: Kajian karakter lanskap berbasis lirik tembang tradisional sunda
Abstrak: ndonesia adalah negara yang memiliki keanekaragaman budaya, salah satunya yaitu kebudayaaan Sunda. Kebudayaan Sunda merupakan kebudayaan tertua di Nusantara. Salah satu yang paling menonjol dalam kebudayaan Sunda adalah tembang Sunda. Tembang Sunda memiliki karakter yang unik dan mewakili kebudayaan Sunda. Ditinjau dari aspek lanskap, tembang Sunda bisa menjadi acuan untuk mengkaji karakter lanskap Sunda. Pengkajian dilakukan berdasarkan informasi yang terdapat dalam lirik-lirik tembang tradisional Sunda. Tujuan penelitian ini ialah untuk mengkaji karakter lanskap melalui elemen-elemen lanskap serta tata letaknya yang berbasis lirik tembang tradisional Sunda. Metode yang digunakan merupakan metode deskriptif kualitatif dengan teknik studi pustaka dan wawancara. Tahapan penelitian mencakup persiapan, pengumpulan data, pengolahan data, dan penyajian hasil. Hasil penelitian menunjukkan adanya tiga lingkup lanskap yang terdapat dalam lirik tembang tradisional Sunda, yaitu lanskap skala regional, lanskap skala permukiman, dan lanskap skala halaman rumah. Lanskap skala regional digambarkan melalui karakter lanskap yang bergunung-gunung dan berbukit-bukit serta sungai-sungai yang mengalir. Karakter lanskap skala permukiman digambarkan dengan adanya rumah berserta pekarangannya, alun-alun, pasar, bangunan bale, makam dan sirkulasi jalan serta letaknya di antara bukit-bukit dan lahan pertanian. Selanjutnya, karakter lanskap skala halaman rumah digambarkan dengan adanya tanaman hias yang harum wanginya dan mencolok warnanya di halaman depan rumah, serta elemen lain seperti leuit, pagar bambu, pagar hidup, empang, tanaman obat, tanaman sayur, jamban, dan sumur.
Keyword: Sundanese culture, Sundanese traditional tembang lyrics, landscape characteristic.

Judul: Kajian Karakteritik Lanskap Sunda Parahiyangan sebagai Model Lanskap Pertanian Berkelanjutan
Abstrak: Agricultural landscape (agroecosystem) is a unified system of ecology and socio-economic and also spiritual-culture that involved in production of foods, shelters, clothes, fibers, biofuels, and other agricultural products. Sustainability of agroecosystem is needed to support the continuity of life, especially for rural communities that relied strongly on agricultural resources. Sundanese society as a rural and mountain society has its own traditional ecological knowledges (TEK). TEK of Sundanese society could be a filter for modernization that negatively affects socio-cultural life of the society itself, as well as damages the nature and environment. Regarding to the role of TEK, it is necessary to do an explorative and descriptive-qualitative study of the various forms of TEK in the context of sustainable agriculture. The qualitative method was used for collecting relevant data to the characteristic of Sunda Parahiyangan landscape. The Data were collected by using Rapid Participatory Rural Appraisal (rPRA) through semi-structured interview, Focus Group Discussion (FGD), and field survey. Data analysis used Landscape Characteristic Assessment (LCA) to analyze character of Sunda Parahiyangan landscape, Knowledge-Based System Methodology to analyze TEK, National Research Council (NRC) and Community Sustainability Assessment (CSA) to analyze sustainable agricultural landscape. The sustainability of agricultural landscape was proposed to achieve USDA’s sustainable agricultural landscape criteria. The study results indicate that the Sunda Parahiyangan agroecosystem has unique characteristics as a result of the adaptation of society to nature and environment through a learning process from generation to generation in a relatively long time period. The character at rural landscape that has been created is a mountainous agricultural landscape type with abundant water resource as a main element. Therefore, Sundanese society called as mountain people (urang gunung) and water people (urang cai). In general, this character is reflected in some Sundanese’s agroecosystems such as kebun-talun (forest garden), sawah (paddy field), and pekarangan (home garden) that located in the surrounding settlement area. Furthermore, kabuyutan concept is one of the TEKs related to understanding and utilization of nature and environment. Kabuyutan is focused on revitalizing the role of forest (mountain) as a buffer of agroecosystem balance by calculating and deciding protected area (leuweung larangan/protected forest and leuweung tutupan/conservation forest). To support the sustainability, integrated between traditional and modern concept by practicing agroforestry system with low external-input and sustainable agriculture (LEISA) system and also applying Islamic management could be applied in utilizing and managing agricultural resources.
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Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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