Judul: Ketahanan Penetrasi dan Kekuatan Geser Regosol dan Latosol di Bawah Tegakan Bambu
Abstrak: Bambu berperan penting dalam menekan erosi dan meningkatkan stabilitas tanah. Penelitian bertujuan mengkaji nilai ketahanan penetrasi dan kekuatan geser Regosol dan Latosol di bawah tegakan bambu pada jarak yang berbeda dari rumpun bambu. Lahan bambu yang diteliti berlokasi di Kebun Pendidikan Cikabayan, Institut Pertanian Bogor. Hasil penelitian menunjukkan bahwa nilai ketahanan penetrasi dan kekuatan geser pada Latosol lebih tinggi dibanding Regosol. Nilai ketahanan penetrasi dan kekuatan geser pada Regosol cenderung semakin besar seiring dengan semakin jauh dari rumpun bambu, sedangkan pada Latosol cenderung semakin besar seiring dengan jarak yang semakin dekat. Perakaran bambu membuat tanah menjadi porous sehingga memudahkan akar untuk menembus tanah. Selain itu, akar bambu dapat menyebabkan adanya penguatan tanah karena kuat tariknya yang tinggi sehingga meningkatkan nilai kekuatan geser tanah.
Keyword: bamboo roots, penetration resistance, shear strength, water content

Judul: Ketahanan Penetrasi dan Kekuatan Geser Regosol dan Latosol di Bawah Tegakan Bambu
Abstrak: Bambu berperan penting dalam menekan erosi dan meningkatkan stabilitas tanah. Penelitian bertujuan mengkaji nilai ketahanan penetrasi dan kekuatan geser Regosol dan Latosol di bawah tegakan bambu pada jarak yang berbeda dari rumpun bambu. Lahan bambu yang diteliti berlokasi di Kebun Pendidikan Cikabayan, Institut Pertanian Bogor. Hasil penelitian menunjukkan bahwa nilai ketahanan penetrasi dan kekuatan geser pada Latosol lebih tinggi dibanding Regosol. Nilai ketahanan penetrasi dan kekuatan geser pada Regosol cenderung semakin besar seiring dengan semakin jauh dari rumpun bambu, sedangkan pada Latosol cenderung semakin besar seiring dengan jarak yang semakin dekat. Perakaran bambu membuat tanah menjadi porous sehingga memudahkan akar untuk menembus tanah. Selain itu, akar bambu dapat menyebabkan adanya penguatan tanah karena kuat tariknya yang tinggi sehingga meningkatkan nilai kekuatan geser tanah., Bamboo plays an important role in supressing erosion and increasing soil stability. The research aims to examine the value of penetration resistance and shear strength of Regosol and Latosol under bamboo stands at difference distance of bamboo clumps. The bamboo land is located in Cikabayan Educational Field, IPB University. The results showed that the value of penetration resistance and shear strength in Latosol was higher than Regosol. The value of penetration resistance and shear strength in Regosol tends to get bigger with the distance from bamboo clumps, whereas in Latosol it tends to get bigger as the distance gets closer. Bamboo roots could make soil become porous so that it could be easier for the roots to penetrate into soil. In addition, bamboo roots could reinforce soil strength because of its high tensile that could increase the value of soil shear strength
Keyword: bamboo roots, penetration resistance, shear strength, water content

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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