Judul: Respon Fisiologis dan Tingkah Laku Domba Garut dengan Pakan Limbah Tauge atau Kangkung Kering sebagai Pengganti Rumput
Abstrak: Garut sheep is commonly reared in West Java and usually fed with grass as the main feed and energy source. However, this grass has low quality and seasonally available, therefore should be an alternative feed such as mung bean sprout waste and dried kangkung. This research aimed to study sheep physiology and behavior as a response to diet and environment. This research used nine garut sheep (I0) with three treatments and replicates. The treatments were P0 (50% grass+50% consentrate), P1 (50% mung bean sprout waste+50% concentrate) and P2 (50% dried kangkung+50% concentrate). Physiological response data were analyzed by analysis of variance (ANOVA) and tested by Tukey post test method, while behavior frequency data were analyzed by Kruskal-Wallis test. Result showed that diet treatment did not affect (P>0.05) the heart and respiration rate, but significantly affected (P<0.05) on rectal temperature. All behaviors parameters were not significantly different (P>0.05) except for resting, grooming, licking strange object, and locomotion were higher in P1 than P0 and P2.
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Judul: Performa Domba Garut dengan Pemberian Pakan Limbah Tauge dan Kangkung Kering Sebagai Hijauan Pengganti Rumput
Abstrak: The improvement of feed quality and raising management can increase the sheep performance. Nowadays, the availability of feed such as good quality grass is relatively expensive; therefore, there should be an alternative feed in place of grass. This research aimed to test the performance of male Garut sheep aged less than one year which was fed by bean sprout waste and dried kangkung as alternative feed. The raising time was two months. The feed treatment consisted of P0 (50% grass and 50% concentrate), (50% bean sprout waste and 50% concentrate), and (50% dried and 50% concentrate). The parameters observed were Daily Weight Gain, feed consumption, feed efficiency, and palatability. This study used complete by random design using feed factors. The data analysis used was ANOVA test, and if significantly influenced, Duncan test would be carried out. The research result showed that different kinds of feed did not have a significant effect on Daily Weight Gain and feed efficiency. However, it had a significant effect on feed consumption and palatability. Based on this research, it can be concluded that in general alternative feeds in form of bean sprout waste and dried kangkung can replace grass since the nutrition content is almost the same and generates similar Daily Weight Gain.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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