Judul: Hubungan Partisipasi Ibu Balita di Posyandu dengan Pengetahuan, Sikap, dan Perilaku Gizi Ibu Balita serta Status Gizi Balita di Kecamatan Tamansari, Kabupaten Bogor
Abstrak: The objective of this research is to examine correlation between mother’s participation in posyandu with knowledge, attitude, behavior and adequacy level of children under-five nutrition in Tamansari, Bogor. This research is part of the research which its title was “a Multi-Approach Intervention to Empower Posyandu Nutrition Program to Combat Malnutrition Problem in Rural Areas” was conducted on February 2012 by using a cross sectional study design. 120 people become sample in this research were selected purposively with sample criteria are (1)have children under-five (male or female 0-60 month), (2)registered as a users of posyandu, (3)ready to be interviewed. The data which used are primary data including characteristic of family and individu sample (big of families, income of families, age, education, and job of sample, characteristic of children underfive (gender and age), mother’s participation in posyandu, knowledge, attitude, behavior of nutrition, food consumption of children under-five, and nutritional status of children under-five. Secondary data including general image of research location. The analysis was carried out with Structural Equation Modeling (SEM). Based on the analysis of SEM, mother’s participation has a significant effect on the level of nutrition knowledge (T-value =-2.59E16). Nutrition knowledge has a significant effect with nutrition attitude (T-value = -3.8323). Nutrition attitude has a significant effect with nutrition behavior (T-value = -3.8323). Nutrition knowledge has a significant effect with nutrition behavior (Tvalue = -3.8323). Nutrition attitude has a significant effect with nutritional status of children (T-value = -3.8323). Nutrition behavior has a significant effect with nutritional status of children (T-value = -5.1027)
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Judul: Keberlanjutan dampak penyuluhan gizi terhadap perilaku gizi ibu dan kualitas pelayanan posyandu
Abstrak: Integrated Service Center Post (Posyandu) is spearheading the early detection and the first services in prevention of malnutrition in Indonesia. In terms of quality, there are barriers that often occur in the implementation of activities posyandu is weak “Communication, Information, and Nutrition Education” programs which is one of the cornerstone in nutrition programs at posyandu. Besides the quality of cadres that still low because of the training received still lessalso the educational levels are low, so that cadres can not given education to mothers posyandu users. Thus, the specific objective of this study were to:(1) Identified the characteristics of mother and children under five years in posyandu; (2) analyzed impact‟s sustainability of nutrition education to knowledge, attitudes, and practices of mothers toddler in posyandu; (3) analyzed correlations of health history and nutritional status children‟s under five; (4) analyzed correlations of knowledge, participation of cadres, and service quality in posyandu; (5) analyzed the impact‟s sustainability of nutrition education to service quality of posyandu; (6) analyzed the sustainability of nutrition education program in posyandu. The design in this study was quasi-experimental. This study is part of the researchs in collaboration with Nestle Foundation (NF) entitled “A Multi- Approach Intervention to Empower Posyandu Nutrition Program to Combat Malnutrition Problem in Rural Areas” (Khomsan et al. 2012). Location of the study is Sukajadi and Sukaluyu village, Tamansari District, Bogor Regency, West Java was conducted in January 2012 until September 2013. The villages were allocated simples randomly as control and intervention group. Nutrition education given to mother‟s toddler and cadre of posyandu was 6 times with frequency 2 times in one month. Data collection consisted of baseline (before intervention), endline (after intervention), and follow-up (four months after endline). Data was analyzed descriptively using Microsoft Excell 2007 and inferentialy using Analysis of Variance (ANOVA) by SPSS 16 softwarer. The results showed that nutritional education impacts to knowledge, attitudes, and practices of mother‟s toddler was significant (p<0,05). Trend change scores tend to declined at follow-up. However, these showed better than baseline. Therefore, it can be said that nutrition educatioan was sustainable. The results showed that most of the mothers toddler in intervention group (93,3%) expressed a desire to stay abreast/ bring the child to the age of five-yearold child to posyandu at the time of the endline. The ANOVA results indicated a difference in the planned visit to the posyandu to five-year-old childs at baseline and endline in the intervention group (p<0.05). This means education of participation posyandu in the form of motivational visit to posyandu was able to increased the knowledge about the importance posyandu. So, desire of mother toddler to keep abreast posyandu was increased. Based on health status, toddlers in the control group and the intervention had a history of disease respiratory infection was higher than the fever, diarrhea, skin diseases, and other (smallpox and convulsions). Nutrition and health educations given to cadres and mothers toddlers whose were indirectly able to change the trend of the respiratory infection incidences. The results showed that a decline in 1-month history of respiratory infection in the intervention group to
Keyword: nutriton education, nutrition behavior, service quality, posyandu, sustainability

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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