Judul: Histopatologi Ikan Kerapu Bebek (Cromileptes altivelis) yang Terinfeksi Iridovirus
Abstrak: The humpback grouper (Cromileptes altivelis) is a coral reef fish distributed throughout tropical Asia. The fish has high economic value. However, the production of humpback grouper has been affected by viral diseases including Iridovirus infection. The disease caused mass mortality and decreased the quality and value of humpback grouper. The purpose of this study was to examine the histopathological changes of humpback grouper infected by Iridovirus. The Iridovirus infected grouper samples were received from fish farming in floating net cages in the Mandeh waters, West Sumatra. The fishes showed dark discoloration of the skin, splenomegally and Iridovirus positive PCR test results. The tissues of brain, eye, gill, heart, spleen, intestine, pancreas, liver, kidneys, sceletal muscle, and skin were received in 10% buffered formalin. The histopathological examination showed pathognomonic large intracytoplasmic basophilic inclusion bodies in hematopoietic tissue of spleen and kidney, lymphoid cells were observed in choroid layer of eye and in liver sinusoid. The enlarged cells were present in high numbers in kidney, spleen and liver. Smaller numbers were present in eye, gill, intestine and brain, and none in the heart, pancreas, muscle and skin. Other findings correlated with Iridovirus infection were neuronal ischemic, necrosis of intestinal epithelial cells, necrosis of cardiomyocytes, necrosis of hematopoietic cells of liver and kidney. The literature studies resume that pathogenesis of this viral infection begin with the viral entry through the skin ulcer, then the virus spread systemically in the blood circulation, infect the hematopoietic cells in the spleen and kidney as well as the other cells.
Keyword: pathogenesis, Iridovirus, inclusion body, histopathology, grouper

Judul: Viabilitas juvenil ikan kerapu bebek Cromileptes altivelis terhadap infeksi bakteri Vibrio alginolyticus setelah diberi pakan kadar Fe berbeda
Abstrak: Humpback grouper (Cromileptes altivelis) culture potentially against the problem of pests and diseases.Vibrio bacteria which causes vibriosis is the main problem for humpback grouper culture industry which cause the death of fish. The use of iron as a mineral that works in the blood cells could be expected to affect viability of humpback grouper against the disease. The fish was cultured in 60x40x50 cm aquarium reared for 40 days, and stoked at a density of 10 ind./aquarium, it weight was 9.44±0.36 gram/ind. The experiment diet contained different iron supplementation level which are 0 ppm, 100 ppm and 500 ppm. The feed was given 3 times daily at a satiation. After 40 days culture feeding, the fish was exposed in fresh water for 10 minutes and injected by 108 CFU/ml Vibrio alginolyticus. The feeding of iron supplementation (Fe) 0 ppm, 100 ppm, and 500 ppm did not significantly affect specific grow rate and survival rate of humpback grouper before the bacterial infection. After the bacterial infection, the fish fed by diet containing 100 ppm Fe had better viability.
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Judul: Natrium lignosulfonat sebagai agen pendispersi pada mortar
Abstrak: Lignosulphonate, a modification product of lignin waste from pulp and paper industry can be used as water reducer in mortar mixture. This substance can influence the mechanical characteristic of mortar such as diameter of flow, flexural strength, compressive strength, and setting time of cement paste. Lignosulphonate shows it function as dispersant on cement hydration at 3 days that analyzed with x-ray difractometer. Research result show that flow of fresh mortar rise with increase concentration (from weight of cement) of synthetic sodium lignosulphonate (NaLS). It show that mortar homogenity also increasing. The result of flexural strength and compressive strength test use synthetic NaLS 0.1-0.3% from weight of cement show that addition synthetic NaLS 0.2% is the best result because it has the biggest of flexural strenght and compressive strength value. Initial setting time of cement paste increase, about 50% with addition of synthetic NaLS 0.2%. Whereas the final setting time decrease, about 8%. Cement hydration test show increase hydration product peak, Ca(OH)2 dan calcium silicate hydrate (C-S-H).
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