Judul: Engineering technology of micrografting and microcutting in mangosteen (Garcinia mangostana).
Abstrak: The purpose of this study was to assemble propagation technology in mangosteen plant using micrografting and microcutting. This study consisted of four experimental steps, 1) in vitro bud induction technology development from bud explants of mangosteen seedling shoot, 2) physiology and anatomy studies of micrografting in mangosteen, 3) in vitro medium manipulation on mangosteen microcutting; 4) optimization of microcutting acclimatization on mangosteen with sterile porous medium technique (MSP) / in vitro soil-less propagation (IVS). The results of experiment I showed that the best medium for shoot induction phase was MS + BA 4,0 + TDZ 0,2 mg/l, MS + BA 8,0 BA + TDZ 0,2 or MS + BA 4,0 mg /l. Medium multiplication could not obtain shoot multiplication, only addition of nodes and elongation of shoots. The best medium for shoot elongation was medium MS + BA 1 mg/l + KIN 1 mg/l. Experiment II showed that the rootstock derived from a single intact seed germination was better success than other treatments in almost all the observed variables, such as flush shoot that was better success than dormant shoot. There are several factors that determine the success of micrografting, which is width of meristem encounter between the rootstock and scion, the equal cell growth rate between rootstock and scion cells, as well as the similarity in size and structural congeniality between rootstock and scion. The results of anatomical tissue observation (4 months after micrografting) indicated that there was a good graft union on micrografting. Roostsock and scion xylem tissue were fused perfectly. Experiment III results showed that the concentration of 25% MS medium concentration on various substrates with or without the addition of IBA might cause 50-80% of microcutting rooted with the root length between 1,8 to 4,38 cm. Experiment IV results showed that microcutting carried out using MSP technique could grow roots, even at the microcutting that were untreated with root growth stimulating substances. Microcutting that planted using a MSP technique with IBA and Rootone-F treatments at varied concentrations could cause mangosteen microcutting 100% rooted with the root length from 3,44 to 6,88 cm.
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Judul: Studies on anatomical and physiological aspects of mangosteen graftings with several relatives of Clusiaceae.
Abstrak: Pertumbuhan pohon manggis (Garcinia mangostana L.) dikenal sangat lambat. Usaha mempercepat pertumbuhan tanaman sejak dari pembibitan dilakukan dengan bibit-semai mau pun bibit-sambung. Pertumbuhan vegetatif bibitsemai di lapangan lebih hat, tetapi bibit-sambung lebih cepat berbuah. Penelitian ini mengkaji faktor-faktor penyebab terhambatnya pertumbuhan bibit-sambung dengan mengamati aspek-stspek anatomi dan fisiologi pada saat penyembuhan luka dan pertautan jaringan pembuluh. Percobaan dilaksanakan dari bulan Maret 2000 hingga November 200 1 bertempat di Tarnan Buah Mekarsari, Cileungsi dan Kebun Citra Ciptaku di Cijeruk, Bogor. Analisis laboratorium dilakukan di Balitbang Botani dan Balitbio Tanaman Pangan di Bogor, dan BATAN di Jakarta
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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