Judul: Pemodelan spasial kerawanan pencurian kayu menggunakan sistem informasi geografis di KPH Madiun Perum Perhutani Unit II Jawa Timur
Abstrak: Salah satu bentuk gangguan hutan yang menyebabkan kerugian yang paling besar adalah masalah pencurian kayu. Selain menyebabkan kerugian material, pencurian kayu juga mengancam kelestarian pengelolaan dan fungsi hutan. Kondisi tersebut di atas menuntut adanya informasi yang cepat, tepat, dan akurat serta mencakup wilayah yang luas guna memformulasikan kebijakan dalam upaya pencegahan, pengendalian, dan pengawasan serta perkembanganya dalam perencanaan program pengelolaan hutan. Perkembangan teknologi saat ini memungkinkan pencapaian tuntutan tersebut, diantaranya adalah teknologi Sistem Informasi Geografis (SIG) yang sangat berguna dalam usaha pengumpulan, penyimpanan, pemanggilan, transformasi dan penampilan data spasial untuk keperluan tertentu. Penelitian ini bertujuan untuk menghasilkan peta sebaran lokasi kerawanan pencurian kayu serta peta peramalan tingkat pencurian kayu sampai tahun 2011 serta pemodelan spasial tingkat kerawanan pencurian kayu di wilayah KPH Madiun Perum Perhutani Unit II Jawa Timur dengan hipotesis bahwa semakin rendah kelerengan suatu tempat maka semakin rawan terjadi pencurian kayu di lokasi tersebut, semakin dekat dengan jalan maka semakin rawan terjadi pencurian kayu di lokasi tersebut, semakin tinggi kelas umur hutan maka semakin rawan terjadi pencurian kayu di lokasi tersebut, semakin dekat dengan lokasi pemukiman maka semakin rawan terjadi pencurian kayu di lokasi tersebut.
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Judul: Pemetaan tingkat kerawananan pencurian kayu di BKPH Sukolilo KPH Pati Divisi Regional Jawa Tengah dengan menggunakan teknik sistem informasi geografis
Abstrak: Pencurian kayu merupakan salah satu gangguan keamanan hutan yang menyebabkan kerugian bagi Perhutani selaku pengelola hutan produksi di Pulau Jawa. Penelitian ini bertujuan untuk menghasilkan peta sebaran lokasi kerawanan pencurian kayu di BKPH Sukolilo KPH Pati Divisi Regional Jawa Tengah dan mengidentifikasi tingkat sosial ekonomi masyarakat desa hutan. Metode yang digunakan adalah analisis teknik Sistem Informasi Geografis, wawancara tidak terstruktur, regresi logistik dan analisis deskriptif kuantitatif. Hasil analisis dari tahun 2010 - 2014 menunjukkan pencurian kayu tertinggi terjadi pada tahun 2014 dengan jumlah kayu yang hilang sebesar 18961 pohon. Faktor biofisik (jarak dari pemukiman, jarak dari jalan, kemiringan lereng, dan kelas umur) mempengaruhi intensitas pencurian kayu sebesar 35.3 % sementara faktor lainnya (segi umur, tingkat pendidikan masyarakat dan jenis mata pencaharian) sebesar 64.7 %. Tingkat kerawanan pencurian kayu di BKPH Sukolilo ada 4 kelas yaitu sangat rendah 2.12%, rendah 30.27%, sedang 12.33%, tinggi 55.28% dan kerawanan sangat tinggi tidak terjadi di BKPH Sukolilo.
Keyword: BKPH Sukolilo, biophysical factor, mapping, teak theft

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable