Judul: Dinamika Sporulasi Genus Fungi Mikoriza Arbuskula Hasil Penangkaran Dari Bawah Tegakan Hutan Tanaman Jabon (Anthocephalus cadamba Roxb Miq.)
Abstrak: The degraded land generally is not productive so land reparation is needed, one of them is establishing forest plant. Developed many forest plants are planted forests jabon (Anthocephalus cadamba Roxb Miq.). Arbuscular myccorhizal fungi (AMF) could increase jabon growth besides fertilizing. Information about AMF diversity and sporulation genus in jabon is still limited. Therefore, the study of AMF sporulation genus dynamics of jabon forest plants needs to be done. The method of this research is picking by sampling the soil from jabon rizosfer and culture pot media. The results showed that the spores density in November until Desember were increased and Desember until January were decreased on Sorgum bicolor and Pueraria javanica. AMF genus that found was Glomus, Acaulospora, and Scutellospora with main genus was Glomus. Effective types of host plants for trapping AMF is Sorgum bicolor.
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Judul: Studi Keanekaragaman Genus Fungi Mikoriza Arbuskula di Sekitar Perakaran Pohon Jabon (Anthocephalus cadamba Robx Miq.) Alami
Abstrak: Mycorrhiza is a mutualism symbiotic between fungi with high levels of plant roots, one of the soil fungi that has a high rate of spread is AMF. Many studies that discussed the use of AMF to increase plant growth, such as jabon.However the research that related of AMF diversity on jabon plant also needs to be done. This research is done by AMF trapping, firstly AMF trapping is taken from various location of soil smpel in Indonesia. After that one, isolation, characterization, and identification spores that have been found in Forest Biotechnology Laboratory PPSHB IPB, and Soil Microbiology Laboratory Research and Development Center of Forest Resources Bogor. AMF genus are found around the jabon roots namely Glomus, Acaulospora, Gigaspora and Scutellospora. Genus that dominated is the genus Glomus. The efective host plant for AMF trapping is Sorgum bicolor, because the Sorgum bicolor has roots that are more responsive than Pueraria javanica
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Judul: Pengujian Virus Newcastle Disease Gen VII Isolat Lapang Sebagai Virus Standar Pada Uji Tantang Vaksin
Abstrak: This study was conducted to determine the standard of Newcastle disease virus Gene VII field isolate as a virus challenge to vaccine test. A virus suspension that has previously been tested by HA test then titrated with EID50 using 30 Specific Pathogen Free (SPF) of 10 day old chicken embryonated eggs. In addition, chicken lethal dose50 (CLD50) test were used to determine the virus titer that could cause 50% death of the chicken population. The CLD50 test was used 25 day-old-chickens (DOC) divided into 5 groups. The virus was diluted from 10-1 to 10-8. The 104 to 10-8 dilution of NDV was infected to every group of chicken via eyedrop and intranasal with 0.1 ml/chicken dosage. Haemagglutination test showed that the allantoic fluid were contain high titers of NDV is 512 HAU. The infective NDV that had been titrated by EID50 test showed the virus titers is 109 EID50/0.1 ml or 1010 EID50/ml and the virus titers of CLD50 test e.i 106.48 CLD50/0.1 ml or 107.48 CLD50/ml. These results can be concluded that the NDV Gene VII field isolates were most appropriate to be used as a standard virus to vaccine test
Keyword: Newcastle Disease Virus Gene VII, HA tes, EID50 test, CLD50 test