Judul: Kajian Metoda CHAlD dan CHAlD exhaustive sebagai analisa  pohon berstruktur, Studi Kasus Frekwensi serangan Asrna pada  rnurid Sekolah Dasar Kecamatan Cipayung - Jakarta Tirnur
Abstrak: Metoda CHAlD adalah salah satu tipe metoda AID ( Automatic Interaction Detection ) yang diajukan oleh Kass (1980 ) merupakan metoda eksplorasi data yang rnenangani data kategorik. CHAlD exhaustive diajukan oleh Biggs et al (1991), mengemukakan algoritma penyekatan alternatip atas metoda CHAID atas peubah dengan jumlah kategori banyak dan peubah dengan kategori mengambang ( floating ) .
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Judul: Penentuan Penciri Keragaman Konsumsi Pangan Rumah Tangga Rawan Pangan Dengan Metode Chaid Dan Exhaustive Chaid
Abstrak: Keragaman konsumsi pangan merupakan salah satu faktor yang menentukan status gizi individu juga masyarakat. Keragaman pangan individu dipengaruhi oleh karakteristik rumah tangga. Penelitian ini bertujuan untuk menentukan penciri keragaman pangan pada rumah tangga rawan pangan di Kabupaten Bogor. Metode yang digunakan adalah Chi-Squared Automatic Interaction Detection (CHAID) dan exhaustive CHAID. Metode CHAID merupakan analisis non parametrik yang dapat digunakan untuk melihat keterkaitan antar peubah yang memiliki peubah respon kategorik. Output dari analisis CHAID adalah pohon klasifikasi. Dasar pengambilan keputusan metode CHAID menggunakan nilai khi-kuadrat. Metode exhaustive CHAID merupakan pengembangan dari metode CHAID. Hasil penelitian menunjukkan bahwa karakteristik rumah tangga yang menjadi penciri keragaman konsumsi pangan pada rumah tangga rawan pangan adalah agroekologi, status pekerjaan ibu, dan jenis pekerjaan kepala rumah tangga. Peubah yang menjadi penciri keragaman konsumsi pangan pada metode CHAID tidak berbeda dengan metode exhaustive CHAID. Perbedaan terletak pada jumlah kelompok yang dihasilkan pada jenis pekerjaan. Jenis pekerjaan pada metode CHAID terbagi menjadi 3 kelompok sedangkan pada metode exhaustive CHAID terbagi menjadi 4 kelompok. Hal ini terjadi karena koefisien pengali Bonferroni pada metode exhaustive CHAID telah dilakukan penyesuaian sehingga menghasilkan penduga dengan tingkat kepercayaan yang lebih besar.
Keyword: CHAID, exhaustive CHAID, HDDS, keragaman konsumsi pangan

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable