Judul: Water economic value change analysis caused landuse changing case study in Cidanau Watershed in Banten Province
Abstrak: Cidanau is the most important watershed in Banten Province because it has provided economic activity in Serang and Cilegon regions. The users of water services from Cidanau watershed are farmers, households, fisheries, small scale industries, water industries and Krakatau Tirta Industries. Those have been analized to find total economic values of Cidanau water. The aims of the reaserch are to analize water economic value by market pricing and gathering cost, to analize factors influencing water demand curve, and to analize landuse changes of Cidanau and its relationships with water economic values changes from 1997 to 2004. Landsat imaging and GIS of Cidanau have been used the research. The methods of the research are use gathering cost and market pricing to find water values from the users. While the GIS and Remote Sensing have been used to detect landuse changes in Cidanau watershed from 1997 to 2004. Both water values and landuse changes have been analized to find relation the others. The results of the reaserch allows that firstly, total water economic value in 1997 increase Rp 52.006.323.743 per year and in 2004 increase Rp 126.456.999.536 per year. Secondly, landuse of Cidanau has changed in 1997 to 2004; bare land changed is decrease 35.56 %, paddyfield is increase 0.59 %, forest is decrease 4.2 %, agroforest is increase 21.072 %,, shrubs is increase 28.25 %, and wet lands is decrease 9.39 %..
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Judul: Perubahan Karbon Tersimpan Atas Permukaan di Daerah Aliran Sungai (DAS) Cidanau Provinsi Banten.
Abstrak: Cidanau watershed is one area which has an important contribution to the economic development in Banten province. DAS Cidanau has great potential to absorb carbon. In the year 2013 – 2014, payment programs for environmental services in DAS Cidanau has been carried out for 5 of forest farmer groups by PT Krakatau Tirta Industri as a buyer of water. The study was conducted to estimate the carbon stock changes due to land cover changes. Land cover changes was analyzed based on timeseries Landsat Imageries using object-based image analysis (OBIA) method. Meanwhile, 5 pools of above carbon stock were measured by allometric equation (undestructive sampling). The result showed that there 12 different types of land cover, namely upland forest, swamp forests, agroforestri, forest plantations, rubber plantations, shrubs, grass marshes, fields, farmlands, open land, housing, and water bodies. In 2000, 2007 & 2014 carbon stocks in DAS Cidanau accounted for 725,455.99 ton, 733,813.02 ton and 751,818.67 ton, respectively.
Keyword: biomass, above ground carbon, land cover change, allometric equation, watershed

Judul: Excretory/secretory antigen of ascaridia galli L3 stage to trigger yolk immunoglobulin in laying hens
Abstrak: Ascaridiosis caused by the nematode Ascaridia galli. A study was carried out to characterize excretory/secretory antigen of A. galli from larval stage to trigger anti-ascaridiosis yolk immunoglobulin in laying hens. A. galli L3 were recovered from intestines of 100 heads chickens 7 days after oesophagus inoculation with 6000 L2. L3 recovered in this manner were cultured (5 – 10 ml-1) in flasks containing rosswell park memorial institute (RPMI) 1640 media, pH 6.8, without phenol red. Cultures were incubated at 370C in 5% CO2 and culture fluid was collected after 3 days in culture. To prepare excretory/secretory antigen, culture medium were concentrated with vivaspin 30.000 MWCO, and antigen protein concentration were counted as described in Bradford method. The molecular weight of excretory/secretory antigen was determined with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). Active immunizations were applied intra muscularly of chickens with an initial dose of 80 μg. The immunizations were repeated three times with dose of each 60 μg with an interval of one week. The first immunizations were excretory/secretory antigen mixed with fruend adjuvant complete and subsequently mixed with freund adjuvant incomplete. The chicken’s eggs were collected from 49 day after immunizations. Anti-ascaridiosis yolk immunoglobulin (IgY) was extracted from egg yolks by means of ammonium sulphate and purified using fast performance liquid chromatography (FPLC). The purity of anti-ascaridiosis IgY was determined by Bradford method (λ = 280 nm). Antibody was detected by agar gel precipitation test (AGPT) and enzyme linked immunosorbant assay (ELISA). Antigen were detected with immunohistochemistry. Chickens were challanged with dose of 1000 L2 A. galli. Passive immunizations were applied ten times with dose of each 0,875 mg IgY with an interval of one day intra orally. IgY anti-ascaridiosis was tested against A. galli survival in vitro.
Keyword: Unggas, Isolasi dan karakterisasi antigen, Purifikasi imunoglobulin yolk, Pengukuran antibodi ayam petelur, Metode Imunohistokimia, Ascaridiosis