Judul: Deteksi Keberadaan Antibodi Anti-Escherichia coli di Dalam Serum Sapi Neonatus yang Diberi Kolostrum dengan Metode Enzyme-Linked Immunosorbent Assay (ELISA)
Abstrak: The aim of this research is to study the present of antibody against E. coli in the sera of neonates calf which given colostrum and challenged by enterotoxigenic Escherichia coli (ETEC) K99. Eleven neonates calves were clinically healthy kept since birth until the age of one week.This neonates calves are divided into two groups, first group is colostrums group (8 calves) and the other is non-colostrum group (3 calves). Colostrum group were given colostrum on age 0-72 hours with 12 hours interval, whereas non-colostrum group are given cow milk at the same time. Each group were challenge by ETEC K99 5X1010 CFU emulsion it in alhydrogel peroral at the 12 hours of age. Blood sample were taken from jugularis vein on age 0th, 12th, 24th, 48th, 72th, and 168th hours. The present of antibody in the sera detecting by indirect ELISA (Enzyme-Linked Immunosorbent Assay Method). The result showed of the highest concentration of antibody in the sera at 24 hours of age for colostrums group, whereas no antibody against E. coli were detected for non-colostrums group . Based on the results, we concluded the concentration of antibody in colostrum can detection at 24 hours until one week after given colostrum. The highest concentration of antibody anti- E. coli in 24 hours after given colostrum.
Keyword: colostrum, neonates calf, antibody anti-E. coli, sapi neonatus, Kolostrum,

Judul: Deteksi Antibodi Anti-Escherichia coli K99 Dalam Kolostrum Induk Sapi Friesian Holstein Post Vaksinasi Escherichia coli Polivalen dengan Teknik
Abstrak: MIZWAR AMANSYAH. B04051631. Detection of Anti-Escherichia coli’s antibody for Friesian Holstein’s cow colostrum Post E. coli Vaccination with ELISA Method. Under directed: ANITA ESFANDIARI dan SRI MURTINI. This experiment was conducted to detect Escherichia coli (E. coli) K99 antibody in colostrum of Friesian Holstein’s cows vaccined by E. coli K99. Six pregnant cows were vaccined three times at 8, 6, 4 weeks before parturition. The vaccine was given by intramuscular with 5 ml/ animal doses. The colostrum was collected until 5th day after parturition. Immunoglobulin Gamma (IgG) antibody in the colostrum was detected using indirect ELISA method. The result of research shown that antibody can detected from first until colostrum 9, with concentration about 1,054 – 1,129 μg/100 μl. The highest colostrum concentration there in colostrum 1 with 1,129 ± 0,005 μg/100 μl. The conclusion of this research is pregnant cows which given vaccine E. coli polivalen can produce of spesific antibody from E. coli K99 in colostrum first until colostrum nineth with IgG concentration have decrease from colostrum first until colostrum third. Key word: Escherichia coli K99, bovine colostrum, ELISA
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Judul: Kekuatan Sambungan Tarik Tiga Jenis Kayu Menurut Kombinasi Jumlah dan Diameter Paku.
Abstrak: Kayu telah lama dikenal sebagai bahan baku utama dalam konstruksi. Dalam pemakaiannya, sambungan atau buhul pada kayu terutama muncul karena alasan geometrik dan keterbatasan ukuran panjang kayu yang tersedia. Sambungan merupakan titik terlemah dalam konstruksi bangunan. Maka harus diupayakan agar titik sambungan hanya menerima gaya tarik atau tekan aksial saja. Kekuatan tarik sambungan perlu mendapat perhatian karena kekuatannya yang kecil, sehingga sulit untuk menyamai kekuatan balok atau batang utamanya.
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