Judul: Potensi aktinomiset endofit dalam mengendalikan penyakit hawar daun bakteri pada tanaman padi (Oryza sativa)
Abstrak: Bacterial Leaf Blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is the most damaging disease in lowland rice growing areas in Indonesia. Endophytic actinomycetes provide advantages to the host plant through enhancement of plant physiological activity or through other modes of action and serve as source of agroactive compounds which can be used as biocontrol agent. The objectives of this experiments were to examine the potency of endophytic actinomycetes in controlling BLB using in planta-in vitro approaches, to characterize and identify the selected endophytic actinomycetes as well as to confirm their colonization in rice plant tissue. To obtain the objectives, the study was divided into three steps: (1) to evaluate the ability of endophytic actinomycetes in controlling BLB based on in planta (field and the greenhouse) experiments, (2) to study the biological control characters of endophytic actinomycetes using in vitro approach, (3) to characterize and identify the selected endophytic actinomycetes and their colonization in rice plant tissue. The field experiments were conducted in dry and wet seasons. The results showed that indigenous endophytic actinomycetes PS4-16 applied by seed coating and soaking techniques suppressed natural infection of BLB during dry and wet season experiments.Area under disease progress curve (AUDPC) values of PS4-16 in dry season and wet season were 1458 and 1923 respectively. Those AUDPC values were significantly different to negative control but no significantly different to the application of sinthetic bactericide. The application of endophytic actinomycetes PS4-16 under dry season could increased rice yield by 17% compared to positive control. The results of green house experiment showed that inoculation of endophytic actinomycetes AB131-1 on rice var. IR64 had a better growth than PS4-16 (21,8%). Endophytic actinomycetes AB131-1 inhibited the BLB better than PS4-16. Based on an in vitro mechanism study, all of the actinomycetes examined showed antibiosis activity against Xoo patotype IV and had a broad antimicrobial spectrum against several Gram positive and negative bacteria as well as inhibited fungal pathogens. Based on their different colony morphology observation, which produced aerial myselia and had spiral spore forming observed under both light and Scanning Electron Microscope, the selected endophytic actinomycetes AB131-1, AB131-2, PS4-16 and LBR02 belonged to the genus Streptomyces spp. The 16S rDNA sequence of AB131-1 showed 89% closed relationship with Streptomyces sp. The AB131-1, AB131-2, PS4-16 and LBR02 were able to colonize rice plant tissue which confirming them as endophytes.
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Judul: Potensi Beberapa Bakteri Penghambat Pertumbuhan Xanthomonas oryzae pv. oryzae Penyebab Penyakit Hawar Daun Bakteri pada Tanaman Padi
Abstrak: Bacterial leaf blight (BLB) disease caused by plant pathogenic bacteria Xanthomonas oryzae pv. oryzae (Xoo) could lead to death of rice plants. Control of plant pathogenic bacteria can be performed using biological control agents. The aim of this research was to study the inhibitory ability of eight isolates of biocontrol bacteria against Xanthomonas oryzae pv. oryzae (Xoo) under greenhouse conditions. Isolates of biological agents used in the study were obtained from IPB Culture Collection (IPBCC), Department of Biology, IPB and microbial gene bank collection of Indonesian Center for Agriculture Biotechnology and Genetic Resources (BB Biogen), consisting of Pseudomonas aeruginosa C32a and C32b, P. fluorescens ATCC 13525 Pf , Serratia marcescens E31, Bacillus sp. I.5, B. cereus I.21 and II.14, and B. firmus E65 isolates. The methods used in the research i.e. hypersensitivity test, antagonistic test of biocontrol bacteria to Xoo, and in vivo application of biological agents in the greenhouse condition. Hypersensitivity test on tobacco plants using C32a and C32b inoculums showed characteristics of slightly leaf yellowing but did not cause necrosis. Injection using Xoo inoculum showed necrosis on tobacco leaves. Antagonist isolates i.e. C32a, C32b, Pf, I.21, and I.5 showed inhibitory activity against Xoo, whereas others isolates did not show inhibitory activity. In greenhouse experiments IR 64 rice plants were sprayed with biological control agents (107cfu/ml) at 7 days, 14 days, 28 days, and 42 days after planting. The results showed that C32a isolate could suppress better the lesion length of BLB than that of chemical control (copper sulphate). Growth measurement of rice plants were assessed on plant height, number of tillers, panicle number and grain weight. Rice plants spraying treatment with isolates E65, E31, C32a, C32b, I.21, Pf, and I.5 showed no difference with control of sterile distilled water. Spraying with each suspension isolate of I.21, E31, C32a, Pf, and C32b indicated no different in the panicle number, in addition rice production was higher in C32a, Pf, C32b, and I.21 treatments. Spraying with C32a isolates produced the highest grain production compared with that of other isolates.
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Judul: Cycle Hamilton pada Graf Lengkap, Graf Regular, dan Graf 2-Connected 4-Regular Berorder Kurang dari Sepuluh
Abstrak: Suatu graf disebut graf Hamilton apabila graf tersebut memuat cycle Hamilton, yaitu cycle yang melewati setiap vertex tepat satu kali. Menentukan keberadaan cycle Hamilton pada suatu graf dapat menggunakan syarat cukup yang berupa teorema-teorema yang telah diperkenalkan sejak tahun 1950-an. Beberapa syarat cukup yang dibahas dalam karya ilmiah ini adalah syarat cukup yang berhubungan dengan graf lengkap dan graf regular. Suatu graf lengkap berorder 𝑛������ ≥ 3 dan graf r-regular berorder 𝑛������ ≤ 2𝑟������ + 1 merupakan graf Hamilton, Karena kedua graf tersebut memuat cycle Hamilton. Selanjutnya, syarat cukup tersebut digunakan untuk menentukan keberadaan cycle Hamilton pada graf 2-connected 4- regular berorder kurang dari 10.
Keyword: graf Hamilton, graf regular, graf lengkap, graf 2-connected 4-regular