Judul: Relationship of Abundance and Diversity of Soil Fauna with Land Position in the Toposequence of Pangrango Mount Bogor
Abstrak: Fauna tanah sebagai salah satu komponen penyusun ekosistem memiliki peran penting dalam proses siklus hara dan perbaikan sifat tanah. Kelimpahan dan keanekaragaman fauna tanah sangat dipengaruhi oleh faktor biotik dan abiotik. Perbedaan topografi dapat menyebabkan adanya perbedaan populasi dan keanekaragaman fauna tanah melalui perubahan sifat tanah dan iklim. Lahan pertanian hortikultura Kabupaten Bogor dapat ditemukan pada kondisi topografi dan elevasi yang bervariasi. Hal ini dapat menyebabkan adanya variasi kelimpahan dan keanekaragaman fauna tanah pada lahan pertanian pada suatu toposekuen. Penelitian ini bertujuan mengetahui pengaruh dan hubungan posisi lahan terhadap populasi dan keanekaragaman fauna tanah pada lahan pertanian di toposekuen Gunung Pangrango, Bogor. Lahan pertanian pada toposekuen dibagi menjadi lereng atas (LAT), lereng tengah atas (LTA), lereng tengah bawah (LTB), dan lereng bawah (LBW). Contoh tanah kedalaman 0-15 cm dan contoh serasah pada masing-masing lahan diambil secara acak sederhana masing-masing berjumlah 10 contoh. Fauna tanah diekstrak dengan metode Berlese Funnel Extractor. Hasil penelitian menunjukkan bahwa jumlah taksa tertinggi terdapat pada LBW berjumlah 19 taksa dan terendah pada LTA berjumlah 12 taksa. Jumlah populasi total tertinggi terdapat pada LBW berjumlah 35.233 individu/m2 dan terendah pada LTA berjumlah 4.361 individu/m2. Keanekaragaman fauna pada lapisan tanah tertinggi terdapat pada LTB dan terendah pada LBW sedangkan pada lapisan serasah tertinggi pada LTA dan terendah pada LTB. Posisi lahan mempengaruhi jumlah taksa, populasi dan keanekaragaman fauna tanah. Jumlah taksa meningkat dengan menurunnya posisi lahan. Posisi lahan berpengaruh nyata terhadap terhadap populasi fauna tanah pada lapisan tanah dan lapisan serasah. Populasi fauna tanah berkorelasi negatif dengan posisi lahan sedangkan keanekaragaman fauna tanah berkorelasi positif. Hal ini menunjukkan bahwa populasi fauna tanah meningkat dengan menurunnya posisi lahan dan keanekaragaman fauna tanah menurun dengan menurunnya posisi lahan pada toposekuen., Soil fauna as one of the components of the ecosystem has an important role in the process of nutrient cycling and in improving soil properties. The abundance and diversity of soil fauna is strongly influenced by biotic and abiotic factors. Topographical differences can lead to differences in abundance and diversity of soil fauna through changes in soil and climatic properties. Bogor Regency horticultural land can be found in various topographical conditions and land elevations. This various topographical conditions and land elevations influenced the abundance and diversity of soil fauna along the toposequence. The objective of this research was to figure out the effect and relationship of land position on abundance and diversity of soil fauna on agricultural land in the southeast toposequence of Pangrango Mount, Bogor. Agricultural land on toposequence was divided into upper slopes (LAT), upper middle slopes (LTA), lower middle slopes (LTB), and lower slopes (LBW). Samples were taken at 0-15 cm soil depth and litter samples with 10 samples each by simple random sampling. The soil fauna was extracted by the Berlesse Funnel Extractor method. The results showed that the highest number of taxa was 19 taxas in LBW and the lowest was 12 taxas in LTA. The highest abundance was found in LBW total 35,233 individuals/m2 and the lowest in LTA total 4,361 individuals/m2. The highest soil fauna diversity in soil layer was found in LTB and the
Keyword: agricultural land, elevation, slope, soil fauna abundance, topographic

Judul: Analysis of Soil Physical and Chemical Properties and Their Relationship with the Abundance of Soil Mesofauna and Macrofauna in Mount Gede Pangrango National Park
Abstrak: Kelimpahan fauna tanah dipengaruhi oleh berbagai faktor lingkungan meliputi faktor edafis, klimatis, dan keberadaan vegetasi di atasnya. Penelitian ini bertujuan menganalisis kelimpahan fauna tanah pada ketinggian tempat berbeda serta menganalisis pengaruh lingkungan terhadap kelimpahan fauna tanah. Penelitian dilaksanakan pada tiga ketinggian yang berbeda yaitu 1.550 m dpl, 1.650 m dpl, 1.750 m dpl, serta di lahan terbuka (1.200 m dpl). Hasil uji BNT menunjukkan kelimpahan fauna tanah yang berbeda nyata antara ketinggian 1.650 m dpl dengan lahan terbuka, sementara kelimpahan fauna tanah pada ketinggian 1.550 m dpl dan 1.750 m dpl tidak berbeda nyata pada selang kepercayaan 95%. Tutupan vegetasi turut memberikan kontribusi dalam kelimpahan fauna tanah dan dibuktikan dengan lahan terbuka yang memiliki kelimpahan fauna tanah terkecil. Hasil analisis korelasi menunjukkan bahwa intensitas cahaya dan suhu udara berkorelasi negatif, sedangkan kelembaban udara berkorelasi positif dengan kelimpahan fauna tanah. Faktor yang berkorelasi kuat dengan kelimpahan adalah berat basah serasah dan pH tanah., The abundance of soil fauna is influenced by various environmental factors including edaphic factors, climatic factors, and the presence of overlying vegetation. This study aims to analyze the abundance of soil fauna at different altitudes and analyze the influence of the environment on the abundance of soil fauna. The research was conducted at three different altitudes, namely 1.550 m asl, 1.650 m asl, 1.750 m asl, and in open land (1.200 m asl). The LSD test results showed that the abundance of soil fauna was significantly different between the altitude of 1,650 m asl and open land, while the abundance of soil fauna at an altitude of 1,550 m asl and 1,750 m asl was not significantly different at the 95% confidence interval. Vegetation cover also contributes to the abundance of soil fauna as evidenced by open land having the smallest abundance of soil fauna. The results of the correlation analysis show that light intensity and air temperature are negatively correlated, while air humidity is positively correlated with the abundance of soil fauna. Factors that are strongly correlated with abundance are litter wet weight and soil pH.
Keyword: altitude, climatic, diversity, edaphic, vegetation

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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