Judul: Gambaran Diferensiasi Darah Perifer, Limpa dan Sumsum Tulang Mencit (Mus musculus) sebagai Respon Terhadap Pemberian Vaksin Streptococcus agalactiae yang Diradiasi.
Abstrak: The aim of the research was to study the response of Streptococcus agalactiae irradiated vaccine on mice. Parameters observed were leukocyte differentiation on peripheral blood, number of spleen lymphoid follicles and its diameters and bone marrow size. Twelve 3 week old female mice were divided into 4 groups : K (without treatment), V (vaccinated with S. agalactiae irradiated vaccine without challenge), VT (vaccinated with S. agalactiae irradiated vaccine then challenged with S. agalactiae), T (challenged with S. agalactiae without vaccine). Vaccines (0,2 ml/mice) were injected intra peritoneal with a dose of 108 cfu/ml and challenge was performed by dropping S. agalactiae through the externa orificium nipple canal of the mice. Blood samples were collected from sinus retro orbitalis arteriae every week that were used for blood smear slides. Necropsy was performed at the end of the study to make histopathology slides of the spleen and bone marrow. Parameters observed were: leukocytes differentiation, number of white pulp and its diameters and bone marrow size. Results showed there was an increase number of neutrophils, lymphocytes, monocytes, white pulp, and a decrease in bone marrow size in the V and VT group. This indicates that the vaccine was able to induce an immune response. On the other hand, this vaccine does not cause allergy characterized by a constant number of basophils and eosinophils.
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Judul: Kajian Histopatologis Pengaruh Vaksin Streptococcus agalactiae yang Diradiasi pada Organ Hati dan Ginjal Mencit (Mus musculus)
Abstrak: This research was conducted to study the effect of irradiated vaccine Streptococcus agalactiae as attenuated vaccine (LD50) by gamma iradiation for the prevention of subclinical mastitis. The study was carried out on the liver and kidney of mice (Mus musculus). Twelve female mice were used in this study which divided into 4 groups, negative control (K); positive control (T); vaccine group (V); vaccine and challange group (VT). The treatment were done during pregnancy and challange post parturient (7 days), while vaccination gave during pregnancy with interval one week between first vaccination and boosting vaccine with 108cfu/ml/mice of S. agalactiae suspension a long four weeks. A week after parturient, mice were challange by S. agalactiae isolated from bovine subclinical mastitis case, dropped 50μl 108cfu/ml/teat on to orificium externa of mice mammary teat. One day after word followed by necropsy, samples were collected and fixed by Buffered Neutral Formalin 10% (BNF 10%) and procecced for histopathological slides. The parameters studied were counting of hepatocytes changes as degeneration, inflammation and necrosis both in central and portal vein region. These parameters also observed in kidney on glomerular and tubular part. Data were analized by ANOVA test and followed by Duncan test. The result showed that irradiated vaccine S. agalactiae did not have toxic effect on liver and kidney of mice that can be proved with have no significant different (p<0,05) compared between V group and K group include increase of degeneration, inflamatory cell, and cell death.
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Judul: Kelayakan Usaha Rental Tanaman Hias
Abstrak: Pesatnya pembangunan fisik yang dilakukan oleh Indonesia selama 30 tahun telah menyebabkan semakin sempitnya lahan pertanian. Bangunan pencakar langit dan perumahan telah mengurangi kenyamanan karena lingkungan menjadi tidak asri lagi. Hal ini telah mendorong manusia untuk melakukan penghijauan yang dilakukan baik di luar maupun di dalam ruangan hingga rental tanaman hias berkembang dengan pesat.
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