Judul: Metabolisme prebiotik oleh kandidat probiotik isolat asi sebagai dasar pengembangan produk sinbiotik
Abstrak: Seven Lactobacillus strains isolated from breast milk were evaluated for their ability to metabolite oligosaccharide known as prebiotics, i. e. inulin, FOS, GOS, FOS:GOS (1:9), and inulin:GOS (1:9). The result shows that all Lactobacillus strains were able to grow in medium with prebiotics as carbon sources. The best growth was observed when GOS or FOS were used as carbon source, while inulin was the worst. The best growth in all oligosaccharides was observed in Lactobacillus R23H, followed by R23, B16, and R14. Further study on R23H and R23 shows that there was no difference in metabolizing oligosaccharides between heterofermenter and homofermenter. GOS was the best prebiotic used by the both lactic acid bacteria as shown by rapid decrease of total sugar. The reducing sugar in medium containing GOS was higher than other oligosaccharides. The decrease of total sugar in medium containing inulin was the slowest among the other prebiotics. This indicate that inulin was the most difficult prebiotic to be metabolized by the selected lactic acid bacteria. When inulin mixed with GOS in the ratio 1:9, the rate of decreasing of total sugar in the medium was similar to the medium with GOS as single carbon source, however it cannot be distinguished if the role of inulin took place after GOS disappear. The rate of sugar metabolism was in accordance with the growth of lactic acid bacteria isolates in medium containing prebiotics. Lactobacillus R23 was used in sinbiotic fermented milk product combine with FOS, inulin, and inulin:GOS (1:9). After fermentation, the result show that remaining sugar in skim milk with additional prebiotics was higher than skim milk without additional prebiotics. Remaining sugar on fermented milk product include the amount of prebiotic that will use as carbon source in colon. Combination of potential probiotic Lactobacillus R23 and mixture of inulin:GOS (1:9) could be used to make sinbiotic fermented milk product.
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Judul: Karakteristik Pertumbuhan Bakteri Asam Laktat Indigenous Susu Sapi Segar Sebagai Kandidat Bakteri Probiotik Dalam Saluran Pencernaan in vitro
Abstrak: Functional foods in the form of probiotic yogurt serves as a food that provides health effects, especially in the digestive tract. This probiotic yogurt can maintain the balance the intestinal microflora, especially between beneficial and pathogen bacterials. Terms of the probiotic bacteria concerned to lactic acid bacteria that were able to survive at the digestive tract. The purpose of this study was to investigate the characteristics of lactic acid bacteria indigenous cow’s milk as a candidate of probiotic bacteria. Resistancy of lactic acid bacteria through different pH conditions, in presence of bile salts or antibiotics, were tested in the media that was conditioned as human digestive tract (in vitro). Lactic acid bacteria resistance was seen through a comparison of the original population before the treatment and the population after treatment. The results of this study, bacteria L. acidophilus Y-01, B. longum Y-01 and L. bulgaricus S-01 can be used as candidates probiotic bacteria because it survived in a pH condition (pH 2, 2,5, 3,2 and 7,2), the condition of bile salts, and antibiotics. Although the population of S. thermophilus S-01 and L bulgaricus S-01 decreased after treatment, all four of these bacteria also produce antimicrobial evidenced by the clear zone that formed around the supernatant. This antimicrobial activity showed inhibition to the growth pathogenic bacteria (E. coli, S. Typhimurium, and S. aureus).
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Judul: Penerapan teknik watermarking untuk penandaan hak cipta pada peta vektor dengan format DXF
Abstrak: Drawing Interchange Format (DXF) is one of the general file in the field of the vector image commonly used for making maps. The DXF format is a tagged data representation of all the information contained in an AutoCAD drawing (DWG) file. Tagged data means that each data element in the watermark is preceded by an integer number called the group code. The study was carried out because there is still no tools of labeling the copyright on digital mapping using DXF file. This study aims to generate an application for copyright labeling on vector map with DXF format that can be applied to any types of spatial objects in the map. The technique used is changing the code inside the vector image. The insertion of the watermark is done by utilizing ENTITIES section by multiplying objects for sets of binary values. The results showed that the image after the watermarking insertion process is the same as the original vector image. Embedded watermark is resistant to rotation and scaling, however it has the possibility of one-third to be broken in the attack on specific object. This study has successfully implemented watermarking technique for copyright labeling on the vector map with DXF format.
Keyword: Drawing Interchange Format, Robust Watermark, Tagged Data, Vector Image, Watermarking