Judul: Perancangan Primer Spesifik untuk Mendeteksi Dini Jamur Pangan Ektomikoriza Pelawan
Abstrak: Pelawan mushroom (Heimioporus sp.) is an edible ectomycorrhiza which has significant economical value. The fruiting bodies of the mushroom are firstly developed in the symbiosis system in the mature host plant. The existence of the mycelia in the rhizoplane of the host plant in seedling stage need to be detected to ensure successful colonization of targeted fungi in mature host plant. Therefore, specific primer is needed for early detection. In this experiment, five sequences of ITS rDNA of pelawan mushroom (JPA, JB, JPC, JPD, and JPE) were successfully obtained which size of 743-745 bp and their bases variation was below 3,3%. The five sequences however, had a significant variation when they were compared with other ITS rDNA sequences of pelawan mushroom collection of the Mycology Division and RCBB-IPB laboratory (JPPK, JPPM, and JPPX). Therefore, the primers were designed into two different groups. The first group was primers designed based on ITS rDNA sequences of fruiting body of JPA-JPE. They were PelAF1-Pel5.8SR1 (±230 bp), Pel5.8SF2-PelAR2 (±320 bp), and PelAF1-PelAR2 (±650 bp). The second group was primers designed based on ITS rDNA sequences of fruiting body of JPPK-JPPX. They were PelBF1-Pel5.8SR1 (±180 bp), Pel5.8SF2-PelBR2 (±260 bp), and PelBF1-PelBR2 (±560 bp). The specificity of the designed primers were tested against fungal DNA of Mortierella sp., Penicillium sp., Hypoxylon sp., Fusarium sp., Glomerella sp., Volvariella sp., and Ganoderma applanatum (IPBCC.10.658) as well as DNA from both groups of pelawan mushroom. The results indicated that primers PelAF1-PelAR2 was specific to the first group and primers PelBF1-PelBR2 was specific to the second group of pelawan mushroom, but the other primers could amplify all or some of the fungal DNA tested.
Keyword: ectomycorrhiza, edible pelawan mushroom, Heimioporus sp, ITS rDNA, specific primer

Judul: Analisis Morfologi dan Sekuens ITS rDNA Jamur Edibel Ektomikoriza Pelawan dan Struktur Ektomikorizanya
Abstrak: Ectomycorrhizae is the symbiosis of fungi and specific plant which is characterised by miselium mantled on root, the presence of Hartig net, and extracellular hyphae. Some ectomycorrhizal fungi are edible mushroom, and one of edible ectomyorrhizal fungi in Indonesia is pelawan mushroom. Morphological and molecular analysis of pelawan mushroom has been done. The spore shape is ovoid to ellipsoid with completely reticulate ornamentation. The characteristic of macroscopic and microscopic feature, especially spore shape and ornamentation, drew the description to genus Heimioporus which close to H. retisporus but different in its spore morphology and some cystidial features. The DNA isolated using CTAB method and PCR amplified with ITS1 and ITS 4 primers showed about 700 bp long. Molecular analysis based on ITS ribosomal DNA showed this mushroom was closely related to Strobilomyces retisporus, a basidionym of H. retisporus with similarity under 90%. However, the lack of similar ITS sequences of the same genus made it hard to decide the species judgment based on molecular approach. The ectomycorrhizal structure of root symbiont was also examined. The root has crème colour with monopodially branch type and white extra radical septated hyphae without clamp connection. The section of the root revealed the mantle with plectenchymatous and pseudoparenchymatous hyphae and para-epidermal Hartig net. This research revealed a new information about ectomycorrhizal symbiosis between Heimioporus sp. and Tristaniopsis merguensis.
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Judul: The impact of oil fuel price subsidy policy on fiscal and national income performance
Abstrak: The objectives of this study was to analyze the impact of oil fuel price subsidy policy on fiscal and national income performance. A simultaneous equestions econometric model of oil fuel price subsidy was estimated using Two – Stage Least Squares (2SLS) method and SYSLIN procedure for the data set period of 1987-2005. The validation model of the oil fuel price subsidy policy used NEWTON method and SIMNLIN procedure. The government policy to increase oil fuel price subsidy showed that surplus of balance of trade was decreased, and the economic growth was decreased. However, the number of poor people was decreased because the inflation rate was decreased. The government policy to decrease oil fuel price subsidy showed that surplus of balance of trade was increased, fiscal deficit was decreased, and the economic growth was increased. However, the number of poor of people was decreased because inflation rate was decreased and income was increased. As the conclusion, the government policy to decrease oil fuel price subsidy was need to increase the surplus of balance of trade, decrease the deficit fiscal, increase the economic growth, and decrease the number of poor people.
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