Judul: Land Using Sentinel-2 Imagery in Sukamakmur District, Bogor Regency from 2018-2023
Abstrak: Pertumbuhan penduduk yang pesat mengakibatkan tingkat kebutuhan masyarakat terhadap lahan semakin meningkat sehingga menyebabkan terjadinya perubahan tutupan lahan. Penelitian ini bertujuan untuk mengidentifikasi dan menganalisis perubahan tutupan lahan di Kecamatan Sukamakmur Kabupaten Bogor menggunakan Citra Sentinel-2 tahun 2018–2023. Terdapat 9 kelas tutupan lahan yaitu hutan, sawah, kebun campuran, pertanian lahan kering, pemukiman, lahan terbuka tanah, semak, sungai, dan danau. Kelas tutupan lahan yang mengalami peningkatan adalah tutupan lahan hutan (83,40 ha), pertanian lahan kering (48,94 ha), sawah (6,35 ha), dan permukiman (1,08 ha). Sedangkan kelas tutupan lahan yang mengalami penurunan adalah kelas tutupan lahan semak belukar (80,97 ha), lahan terbuka (40,01 ha), dan perkebunan campuran (18,79 ha). Perubahan tutupan lahan yang terjadi di Kecamatan Sukamakmur Kabupaten Bogor menunjukkan sebesar 73,86% mengalami perubahan ke arah positif dan 26,14% mengalami perubahan ke arah negatif. Perubahan ke arah positif yang lebih besar menunjukkan bahwa cadangan karbon di Kecamatan Sukamakmur semakin meningkat sehingga tanaman mampu menyerap CO2 dari atmosfer lebih banyak dan menyimpannya dalam bentuk biomassa tanaman., Rapid population growth results in the level of people's need for land increasing, causing changes in land cover. This research aims to identify and analyze changes in land cover in Sukamakmur District, Bogor Regency using Sentinel-2 Imagery in 2018–2023. There are 9 land cover classes, namely forest, rice fields, mixed gardens, dry land agriculture, settlements, open land, bushes, rivers, and lakes. The land cover classes that experienced an increase were forest land cover (83.40 ha), dry land agriculture (48.94 ha), rice fields (6.35 ha), and settlements (1.08 ha). Meanwhile, the land cover classes that experienced a decline were the land cover classes of shrubs (80.97 ha), open land (40.01 ha), and mixed plantations (18.79 ha). Land cover changes that occurred in Sukamakmur District, Bogor Regency showed that 73.86% experienced changes in a positive direction and 26.14% experienced changes in a negative direction. Changes in a greater positive direction indicate that carbon reserves in Sukamakmur District have increased so that plants are able to absorb more CO2 from the atmosphere and store it in the form of plant biomass.
Keyword: tutupan lahan, sentinel-2, Sukamakmur

Judul: Perubahan dan Kesesuaian Penutupan Lahan pada Pola Ruang Menggunakan Citra Resolusi Tinggi di Kecamatan Dramaga dan Ciomas, Kabupaten Bogor
Abstrak: Peningkatan jumlah penduduk menjadi faktor perubahan penutupan lahan di Kecamatan Dramaga dan Ciomas. Monitoring perubahan tersebut dilakukan dengan memanfaatkan teknologi penginderaan jauh. Penelitian ini bertujuan mengidentifikasi penutupan lahan menggunakan citra SPOT-7 tahun 2017 dan citra SPOT-6 tahun 2022, menganalisis perubahan penutupan lahan, dan menganalisis kesesuaian penutupan lahan dengan RTRW tahun 2016-2036. Metode yang digunakan adalah klasifikasi visual dengan digitasi on-screen. Akurasi hasil klasifikasi dengan metode digitasi on-screen mencapai 94,38%. Hasil penelitian menunjukkan bahwa perubahan penutupan lahan terbesar di Kecamatan Dramaga dan Ciomas yaitu permukiman dengan peningkatan berturut-turut sebesar 71,65 ha dan 54,66 ha. Namun Penutupan lahan di Kecamatan Dramaga didominasi oleh hutan tanaman rakyat, sedangkan di Kecamatan Ciomas didominasi oleh permukiman. Kesesuaian penutupan lahan tahun 2017 dan 2022 dengan RTRW Kabupaten Bogor menunjukkan peningkatan luas penutupan lahan yang sudah sesuai dengan pola ruangnya. Pada tahun 2022, luas kesesuaian lahan mencapai 79,90% dari seluruh luas Kecamatan Dramaga dan Ciomas., Population growth affects changes in land cover of Dramaga dan Ciomas subdistricts. Monitoring of land cover changes is carried out using remote sensing. This study aims to identify land cover using SPOT-7 imagery in 2017 and SPOT-6 imagery in 2022 to analyze land cover changes and conformity with the 2016-2036 RTRW. The method used is visual classification with on-screen digitization. The accuracy of the classification results with the on-screen digitization method reached 94.38%. The results showed that the most significant land cover change in the Dramaga and Ciomas sub-districts was settlements, with an increase of 71.65 ha and 54.66 ha, respectively. However, land cover in the Dramaga Sub-district is dominated by Plantation Forest, while settlements dominate the Ciomas Sub district. The suitability of land cover in 2017 and 2022 with the Bogor District RTRW shows an increase in the land cover area by the spatial pattern. In 2022, the area of land suitability reached 79,90% of the total area of Dramaga and Ciomas Sub-districts.
Keyword: citra SPOT 6/7, land cover, on-screen digitizing, regional spatial plan

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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