Judul: Penerapan Scrum dengan Game-Based Retrospective: Starfish dalam Pengembangan Aplikasi Zikirin
Abstrak: Tim pengembang aplikasi Zikirin menggunakan metode manajemen pengembangan perangkat lunak Scrum, tetapi masih menggunakan metode sprint retrospective dengan menjawab pertanyaan “Apa yang sudah berjalan dengan baik?” dan “Apa yang harus di-improve?” melalui platform Notion. Metode sprint retrospective ini tidak bisa menjelaskan aktivitas-aktivitas apa saja yang perlu dipertahankan, diperbanyak, dikurangi, dimulai, dan dihindari tim pengembang aplikasi Zikirin pada sprint berikutnya. Pada sprint keenam, peneliti berhasil menerapkan game-based retrospective: Starfish kepada tim pengembang aplikasi Zikirin secara daring melalui perangkat lunak kolaboratif Figma dan Zoom untuk melihat evaluasi sprint yang telah dilakukan oleh tim pengembang aplikasi Zikirin secara lebih rinci dan detail. Metode game-based retrospective: Starfish yang diterapkan pada sprint keenam ini dapat mengetahui aktivitas-aktivitas yang perlu dipertahankan, diperbanyak, dikurangi, dimulai, dan dihindari pada sprint selanjutnya selama proses pengembangan aplikasi Zikirin., The Zikirin application development team utilizes the Scrum software development management method, but still uses the sprint retrospective method by answering the questions "What went well?" and "What needs improvement?" through the Notion platform. This sprint retrospective method cannot explain the specific activities that need to be maintained, increased, decreased, started, and avoided by the Zikirin application development team in the next sprint. In the sixth sprint, the researcher successfully implemented a game-based retrospective: Starfish to the Zikirin application development team through the collaborative software Figma and Zoom to obtain a more detailed evaluation of the sprint conducted by the Zikirin application development team. The game-based retrospective method: Starfish implemented in this sixth sprint can identify the activities that need to be maintained, increased, decreased, started, and avoided in the next sprint during the Zikirin application development process.
Keyword: perangkat lunak, retrospeksi, Scrum, Zikirin

Judul: Mobile Application Development for Shrimp  Pond Water Quality Monitoring and Control System
Abstrak: Udang merupakan salah satu komoditas budidaya perairan di Indonesia. Nilai ekspor sebesar 1.719.172.000 USD pada tahun 2019, membuat udang memiliki potensi besar untuk meningkatkan devisa negara. Potensi yang besar tersebut diikuti dengan resiko yang besar seperti pencemaran lingkungan dan kegagalan budidaya. Hal tersebut menjadi alasan dilakukannya pengembangan aplikasi front-end mobile Water Quality Monitoring and Controlling System menggunakan framework Flutter. Melalui aplikasi yang dikembangkan, petambak udang dapat memantau dan mengontrol kualitas air berdasarkan data sensor yang ditanamkan pada kolam tambak udang. Aplikasi ini dikembangkan menggunakan metode agile dengan framework Scrum. Diawali dengan User Requirement Analysis untuk perencanaan product backlog yang berisikan fitur yang dibutuhkan dan pelaksanaan sprint. Sprint dilakukan melalui 4 tahap, yaitu sprint planning, daily scrum, sprint review dan sprint retrospective. Sprint yang dilaksanakan selama 89 hari (4 sprint) telah menghasilkan aplikasi dengan fitur utama Monitoring, Maintenance dan Aktivasi perangkat IoT., Shrimp is one of the aquaculture commodities in Indonesia. The export value of 1,719,172,000 USD in 2019, makes shrimp has great potential to increase foreign exchange. This great potential is followed by great risks such as environmental pollution and cultivation failure. This is the reason for developing a front-end mobile Water Quality Monitoring and Controlling System application using the Flutter framework. Through the developed application, shrimp farmers can monitor and control water quality based on sensor data embedded in shrimp ponds. This application was developed using agile method with Scrum framework. It begins with User Requirement Analysis for product backlog planning which contains the required features and sprint implementation. Sprints are carried out through 4 stages, namely sprint planning, daily scrum, sprint review and sprint retrospective. The sprint that was carried out for 89 days (4 sprints) has produced an application with the main features of Monitoring, Maintenance and Activation of IoT devices.
Keyword: flutter, aplikasi mobile, Recirculating Aquaculture System (RAS), scrum

Judul: Characterization and Expression of Tomato infectious chlorosis virus Coat Protein Gene in Escherichia coli
Abstrak: Tomato infectious chlorosis virus (TICV) is a member of Crinivirus genus. TICV infects tomato crop in Garut and Cipanas, West Java Indonesia. Tomato initially showed bright interveinal yellowing symptoms. The coat protein gene (CP-TICV) was amplified by RT-PCR from total RNA extracted from infected tomato leaves and the amplified fragment was cloned and completely sequenced. The fragment was subsequently subcloned into the pET-21b expression vector. The recombinant plasmid was transformed to Escherichia coli strain BL21(DE3)pLysS to express the coat protein. The coat protein fused to a 6xhistag, was purified by affinity chromatography using a NiNTA spin column. The identity of the purified protein was confirmed by SDS-PAGE. In this experiment, 792 bp of CP-TICV gene of TICV virus has been successfully cloned, sequenced, and expressed in E. coli. Based on nucleotide sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain strain and 99% identity to North America, France, and California strains and based on amino acid sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain, North America, France strains and 99.2% identity to California strain. CP-TICV showed over expressed in E. coli when it is induced with 1 mM IPTG and incubated at 37oC. Purified CP-TICV-Histag recombinants protein sized 29 kDa based on SDSPAGE analysis.
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