Judul: Efikasi Vaksin DNA Penyandi Glikoprotein Koi Herpesvirus pada Ikan Mas Stadia Benih melalui Perendaman
Abstrak: Koi herpesvirus (KHV) is a herpesvirus which especially infects and causes mass mortality to koi and common carp. Therefore the protection of carp from KHV infection is urgently needed. In this study, we developed an application of DNA vaccine containing gene encoding KHV glycoprotein by immersion method to increase survival of common carp against KHV infection. As many as 400 of 30-day-old common carp juveniles were immersed in the water containing 1.3 x 108 CFU/ml of the killed Escherichia coli cells carrying DNA vaccine. Treatments performed were variation of duration and frequency of DNA vaccine immersion; as follows: 1x 30 minutes, 1x 60 minutes, 1x 90 minutes, 2x 90 minutes, and 3x 90 minutes of immersion treatments. Reverse transcription polymerase chain reaction (RT-PCR) analysis showed that DNA vaccine was successfully expressed among the vaccinated fish. Twenty eight days post vaccination, the fish were challenged by injecting 10-4 ml/fish of KHV. The result demonstrated that vaccination by 30 minutes immersion; allowed the 61% of fish survived, this was significantly different (P<0.05) compared to control (without vaccination), but insignificantly different (P>0.05) compared to those of other treatments. The relative percent survival of vaccinated fish were also similar among different treatments (P>0.05). Vaccination by immersion have increased the survival rate about 2 fold compared to unvaccinated fish control (26.67%). Thus, DNA vaccine was effective delivered by immersion for 1x 30 minutes, and this technique can be useful to level up the resistance to KHV infection in common carp juveniles.
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Judul: Efektivitas vaksin DNA anti-KHV pada benih ikan mas Cyprinus carpio melalui metode perendaman dan perlakuan hiperosmotik
Abstrak: Common carp production has decreased since the mass deaths caused by disease of koi herpesvirus (KHV). Therefore, safe technique is needed and can be applied in bulk with cost efficient. The purpose of this research was verified influence of DNA vaccine anti-KHV with dose of 1,3 x 108 cfu / mL was performed through immersion in different duration after hyperosmotic treatment for 2 minutes treatment to produced the resistence common carp. There were six treatments with three replications, A: vaccination for 60 minutes immersion, B: 90 minutes, C: 120 minutes, positive control: fish were not vaccinated and infected with KHV, negative control: fish not vaccinated and were not infected with KHV, control bacteria: fishes that soaked with bacteria did not containing vaccine. The results showed that vaccination for 60 minutes giving highest relative survival (93.55%). Thus, vaccination by immersion and hyperosmotic treatment effectively improve the survival and relative percent survival of fish, and this method can be an alternative in the early control of KHV infection in common carp and koi.
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Judul: Penerpan Analisis Struktur Jaringan Sosial Pada Jaringan Twitter Akun@matematikaipb.
Abstrak: Twitters is one of the popular social media in cyber space. Each of twitter user has different centrality weights based on its positions i n yhe network structure. The centrality calculations is compared in term of to the number of friendss of users.
Keyword: Twitter, Social Network Analysis, Centrality