Judul: Variasi genetik jati pada hutan tanaman di Jawa berdasarkan penanda RAPD (Random Amplified Polymorphic DNA)
Abstrak: Hutan jati merupakan aset yang sangat penting khususnya bagi Perum Perhutani dan umumnya untuk negara Indonesia. Permintaan yang tinggi terhadap produk hasil olahan kayu jati menyebabkan harga kayu jati terus meningkat. Meningkatnya permintaan akan kayu jati tersebut melebihi jumlah yang dapat diproduksi secara lestari dari hutan produksi. Kondisi tersebut akhirnya memperbesar jumlah antara jumlah pasokan dan permintaan. Kesenjangan antara permintaan dan produksi ini dapat dipenuhi dengan peningkatan produktivitas hutan jati melalui peningkatan kualitas dan kuantitas bibit.
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Judul: Variasi Genetika Jati Jawa Berdasarkan Metode Random Amplified Polymorphic DNA (RAPD)
Abstrak: Information on genetic variation of teak in Java at present is still limited. Existing data mostly were based on isozyme analysis, on few localized population, and using leaves as sample materials. In this study usage of RAPD (Random Amplified Polymorphic DNA) as one of DNA markers, and teak wood as material to study teak genetic diversity could enrich the available information. Samples were collected from nine Forest Management Unit (FMU) representing three provinces in Java, i.e. FMU Banten, FMU Ciamis and FMU Indramayu of West Java province, FMU Cepu, FMU Kebonharjo, FMU Kendal and FMU Randublatung of Central Java province, FMU Bojonegoro and FMU Ngawi of East Java province. Leaves DNA were extracted using CTAB method and woods DNA were extracted using CTAB (Cethyl Trimethyl Amonium Bromide) method, while RAPD analysis were done using four selected primers, i.e. OPY2, OPY9, OPO13 and OPY11 . Generated data were then analyzed using POPGENE version 3.2 and NTSYS 2.02. Results showed that teak genetic diversity in Java as measured from heterozygosity level ranged from 0,0614 (FMU Ngawi) to 0,2753 (FMU Indramayu). Dendrogram based on calculated Nei’s genetic distances clustered the populations into two main groups, i.e. West Java province and Central-East Java province. Amplified DNA from wood comprised only of small size fragments (maximum 500bp) as compared to those of leaves (maximum 2500bp). If wood is going to be used as material for fingerprinting traded forest products, a refinement method in DNA analysis should be done
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Judul: Studi Kasus Liver Neoplasia pada Anjing Shih Tzu
Abstrak: Liver neoplasia is the excessive and uncontrolled replication of hepatocytes that forms nodules visible in ultrasonography. The purpose of this study is to study and discuss the characteristic, diagnosis and treatment of liver neoplasm through the interpretation of ultrasonography and hematology in a Shih Tzu dog. Ultrasonography is conducted with a Chison Q8 ultrasound machine with a 5 to 7 MHz frequency convex probe on lateral recumbency. The blood cells, liver enzymes and nodules sizes were the main parameters in this study. The results in the period of three years from 2021 to 2023 were interpreted and supported with secondary data sources. The Shih Tzu dog is 14 years old in the year 2023 with a body weight of 5.21kg. No obvious clinical signs were observed during previous examinations. However, lethargy, vomiting and anorexia were recorded in its latest examination. Thrombocytosis, granulocytosis, high liver enzymes and hypoglycemia, which are some of the neoplastic clinical manifestations were also presented by this patient. Besides, liver neoplasm, spleen neoplasm and biliary sludge were the sonogram findings of this patient. The liver and spleen neoplasm was observed stable, while the biliary sludge volume fluctuated. The treatment strategy recorded is Ondansetron, Samylin®, Ornipural and Ursodeoxycholic acid.
Keyword: Blood biochemistry profile, Hematology, Liver Neoplasia, Shih Tzu, Ultrasonography