Judul: Analisis Pendugaan Limpasan di Perumahan Tamansari Persada, Bogor
Abstrak: Runoff is affected by various factors, i.e., meteorological factor represented by precipitation and regional flow factor stated the regional drainage physical properties. The change in land characteristics from natural land into developed land will reduce the absorption function in catchment areas and increase runoff. The situation could lead to the occurrence of flood if the planning of drainage at a residential area is not good. It often happens in Tamansari Persada Residence in Bogor. A study was conducted with the objective to identify the direction of runoff (incoming flow and outgoing flow) and to predict runoff in the Tamansari Persada Residence. The methods used in this study were literature review and field study. The material used included Google Earth satellite image acquisition of September 12, 2012, the rainfall data from Atang Sanjaya Weather Station at Bogor from 2003 until 2012 and topographic contour map of the residence. From the observation and analysis, the direction of runoff in the Tamansari Persada Residence followed the topographic from the south to the north. The total discharge of runoff reached 1.31 m3 s-1 in the area of 27.98 ha.
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Judul: Analisis Hubungan Curah Hujan dengan Volume Genangan Air di Perumahan Tamansari Persada, Bogor
Abstrak: Negative impact due to the disappearence of water catchment is the decrease of land capability to infiltrate rainwater causing run off, ponding water or even flooding. This research was conducted with anobjective to know a relationship between rainfall and volume of the ponding water. The location selected in this research was Tamansari Persada Residence in Bogor between March until April 2013. The research was conducted in two phases, which were primary data collection by measuring coordinate points of the ponding water and secondary data collection of daily rainfall. Gridding method based on Surfer 8 was used to calculate the volume. The results showed linear equation relationships between rainfall and volume of ponding water at Location 1 is y = 14.80x - 1.456 with the coefficient of determination 0.558, in Location 2 is y = 30.56x + 96.18, with the coefficient of determination 0.901, and Location 3 linear is y = 53.16x + 46.89, with the coefficient of determination 0.685. The small coefficient of determinations at Locations 1 and Location 3 might be caused by several factors such as ineffective use of time measurement.
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Judul: Primer Spesifik Ikan Hiu Biru (Prionace glauca) Berbasis Marka Cytochrome B untuk Autentikasi Produk Olahan Ikan Hiu
Abstrak: Primer merupakan aspek krusial yang perlu diperhatikan dalam proses mengidentifikasi spesies pada produk olahan hiu berbasis genetik. Jenis marka mtDNA yang dapat digunakan untuk mengidentifikasi species salah satunya adalah gen cytochrome b. Prionace glauca merupakan spesies ikan hiu yang tersebar luas di perairan Indonesia yang jumlah populasinya terus mengalami penurunan. Mendesain primer cytochrome b spesifik Prionace glauca menjadi tujuan dalam penelitian untuk mengidentifikasi adanya spesies tersebut dalam produk olahan ikan hiu komersil. Primer didesain pada fitur Primer-BLAST pada situs NCBI. Primer diujikan melalui proses amplifikasi pada isolat DNA produk olahan hiu yang meliputi ikan hiu asin (A1), abon ikan hiu (B1 dan B2), ikan hiu asap (C1), ikan hiu empal (E1), dan fillet ikan hiu (F1 dan F2) dengan tingkat kemurnian 0.909-2.106 (A260/280). Pada suhu annealing 55oC teridentifikasi ukuran DNA seluruh sampel yang diujia dalah 500 bp. Sekuens dari sampel yang telah diamplifikasi menunjukkan hasil BLAST spesies yaitu Prionace glauca dengan tingkat kemiripan mencapai 99,66%. Kedekatan genetik pada hasil filogenetik terlihat pada kelompok yang terbentuk dengan spesies target Prionace glauca dengan nilai bootstrap 100%., Primer is an essential aspect of the species identification procedure. One of the DNA sourced at cytochrome B mtDNA is recognized suitable as a marker. Shark species that distribute almost in every Indonesia's sea is Prionace glauca. A pair of specific cytochrome b primers were designed with the Primer-BLAST feature on NCBI. The primer got applied at DNA amplification that run to analyze some products labeled as salted shark meat (A1), shark meat floss (B1 and B2), smoked shark meat (C1), shark empal (E1), and shark meat fillet (F1 and F2), have 0,909-2,106 (A260/280) purity rate. The sizes of all DNA samples were identified at 500 bp at 55oC annealing temperature. Prionace glauca was spotted at BLAST result from the sample's sequence with the percent identity up to 99,66%. The phylogenic tree shows a group containing product and Prionace glauca with 100% bootstrap value.
Keyword: Prionace glauca, Cytochrome b, Ikan hiu, Primer spesifik