Judul: Pengaruh Interaksi antara Ayah dan Ibu dengan Anak terhadap Permasalahan Perilaku Sosial Emosi Anak Usia Sekolah.
Abstrak: Penelitian ini bertujuan untuk menganalisis pengaruh interaksi antara ayah dan ibu dengan anak terhadap permasalahan perilaku sosial emosi anak usia sekolah. Lokasi penelitian berada di Desa Ciasmara dan Desa Purwabakti, Kecamatan Pamijahan, Kabupaten Bogor. Penelitian ini mengggunakan desain cross sectional study. Contoh pada penelitian ini adalah anak usia sekolah kelas 4 dan 5 dengan kriteria mempunyai orang tua kandung lengkap dan bertempat tinggal dekat sekolah. Sebanyak 76 anak dipilih secara purposive berdasarkan kriteria. Hasil penelitian menunjukkan bahwa interaksi ayah-anak dan interaksi ibu-anak berhubungan negatif signifikan dengan permasalahan perilaku sosial emosi anak. Hasil menunjukkan bahwa tidak ada perbedaan signifikan antara interaksi ayah dan ibu dengan anak laki-laki dan anak perempuan, dan tidak ada perbedaan signifikan permasalahan perilaku sosial emosi anak laki-laki dan anak perempuan. Interaksi ayah-anak berpengaruh negatif signifikan terhadap permasalahan perilaku sosial emosi anak usia sekolah.
Keyword: anak usia sekolah, interaksi ayah-ibu-anak, permasalahan perilaku sosial emosi

Judul: Pengaruh Sosialisasi Emosi Dan Kecerdasan Emosi Terhadap Perilaku Agresi Anak Usia Sekolah Pada Keluarga Perdesaan
Abstrak: Penelitian ini bertujuan untuk menganalisis pengaruh sosialisasi emosi dan kecerdasan emosi terhadap perilaku agresi anak usia sekolah. Lokasi penelitian terletak di Desa Ciasmara dan Desa Ciasihan, Kecamatan Pamijahan, Kabupaten Bogor. Penelitian ini menggunakan desain cross sectional study. Contoh penelitian ini adalah keluarga lengkap yang memiliki anak usia sekolah. Sebanyak 100 anak dipilih dengan menggunakan teknik acak proporsional, pada dua sekolah terpilih. Hasil penelitian menunjukkan bahwa sosialisasi emosi berhubungan positif dengan kecerdasan emosi. Kecerdasan emosi memiliki hubungan negatif signifikan dengan perilaku agresi. Hasil lain menunjukkan bahwa dimensi penerimaan pada sosialisasi emosi, dimensi kesadaran diri dan pengaturan diri pada kecerdasan emosi berpengaruh negatif signifikan terhadap perilaku agresi anak usia sekolah pada keluarga perdesaan.
Keyword: Anak usia sekolah, kecerdasan emosi, keluarga perdesaan, perilaku agresi, sosialisasi emosi

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable