Judul: Kajian Biologis dan Morfologis Oosit Domba setelah Kriopreservasi dengan Metode Vitrifikasi
Abstrak: Didalam implementasi teknologi transfer embrio serta teknik terkait lainnya, tersedianya embrio serta oosit secara kesinambungan merupakan faktor utama yang harus diupayakan. Penerapan kriopreservasi oosit merupakan salah satu upaya untuk meningkatkan nilai tambah maupun pernanfaatan oosit karena kendala waktu dan jarak pemakaian dapat teratasi. Namun demikian oosit beku hasil kriopreservasi hams memiliki keadaan morfologi yang normal sehingga dapat dipakai untuk produksi embrio. Penelitian ini bertujuan untuk mempelajari
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Judul: Kaji banding viabilitas embrio mencit(Mus musculus albinus) tahap blastosis setelah kriopreservasi dengan metode konvensional dan vitrifikasi open pulled straw (OPS)
Abstrak: Penelitian ini bertujuan untuk mengetahui viabilitas embrio mencit tahap blastosis setelah kriopreservasi dengan metode konvensional (slow freezing) dan vitrifikasi menggunakan metode Open Pulled Straw (OPS). Metode kriopreservasi konvensional dilakukan dengan tahapan: pemaparan embrio di dalam medium pembekuan (PBS + 20% serum + sukrosa 0.5M + ethylene glycol, EG 10%) selama 10 menit, kemudian embrio dikemas di dalam straw dan dibekukan dengan menggunakan mesin pembekuan embrio yang dapat diprogram. Sedangkan vitrifikasi embrio dilakukan dengan tahapan: pemaparan embrio di dalam medium ekuilibrasi (PBS + serum 20% + EG10%) selama 10 menit, dilanjutkan dengan pemaparan di dalam medium vitrifikasi (PBS + serum 20% + sukrosa 0.5M + EG 15% + dimethylsulfoxidase 15%). Selanjutnya embrio dikemas dalam open pulled straw dan langsung dimasukkan ke dalam LN2 (-192 °C). Pemanasan (warming) dan rehidrasi embrio beku dilakukan dengan cara merendam embrio ke dalam larutan sukrosa bertingkat yaitu 0.5M, 0.25M dan 0.1M. Hasil penelitian menunjukkan bahwa viabilitas embrio mencit hasil vitrifikasi mencapai 92% setelah 3 jam dikultur. Hasil ini tidak berbeda nyata (P>0.05) dengan kontrol (100%). Demikian halnya dengan pengamatan 24 jam setelah kultur, persentase blastosis mencapai tahap hatched lebih tinggi (P<0.05) pada kriopreservasi dengan metode OPS (18%) dibandingkan dengan metode konvensional (2,7%). Hal ini menunjukkan bahwa vitrifikasi dengan metode Open Pulled Straw (OPS) dapat digunakan untuk kriopreservasi embrio dengan hasil yang memuaskan.
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Judul: Thermal Characteristics and Dynamics of Tropical Cyclone over Western North Pacific Ocean: A Case Study of Typhoon Songda (Chedeng) 2011
Abstrak: The frequent occasion of Tropical Cyclone (TC) over Western North Pacific (WNP) Ocean lies from 10˚N to 26˚N and 121˚E to 170˚E. Sources of TC development come from warm ocean temperature where latent heat is released during condensation processes and subsequently concentrated in the boundary layer. Relative humidity at 850-700 hPa is responsible to produce the most intense storm. Meanwhile, weak vertical wind shear between 850-200 hPa is one of the dynamical factors that lead to the development of TC. Pressure level of 850-200 hPa is chosen to minimize the thermodynamic effect in the lower troposphere. Typhoon Songda (Chedeng in the Philippines) or TSC lasted from May 18th to 30th, 2011 and strengthened into powerful Super Typhoon stage between May 24th and 27th. Long curving track and the exhibition of all sequences of TC life cycle, TSC is then considered as an ideal case of TC. The objective of the study with case of TSC is to investigate the role of thermal characteristics and dynamics on TC intensity. The research is carried out by using Advanced Research of Weather Research and Forecasting model (WRF ARW model) version 3.3. Two domains are employed in the model. The first domain situate from 02˚N to 40˚N and 118˚E to 149˚E and cover spatial resolution () of 33.1 km. The second domain situate from 09˚N to 27˚N and 119˚E to 132˚E with of 11 km. Timestep of the model () is 120 seconds. The model is supported by the following physical parameterization, i.e., Yonsei University Scheme (YSU) in the boundary layer, Kain-Fritsch scheme (KF) in the cumulus, and WRF Single-Moment 3-class scheme in the microphysics. Initial meteorological condition prior to the formation of the cyclone is generated using WRF ARW standard-release model. Sensitivity of the model output is obtained from a selected single-parameter change scenarios, namely, 10 % to 20 % decrease and increase of wind shear initial data at 850-200 hPa, and by 10 % to 20 % decrease of relative humidity initial data at 850-700 hPa. The result showed that the negative correlations between wind shear and intensity are found in wind shear scenarios. Wind shears of 10 ms-1 which lead to strengthening of the wind intensity in the early period of Typhoon development are found in wind shear scenarios. The effect of wind shear changes on wind intensity is statistically significant in the 20 % wind shear scenarios. During mature stage, weak wind intensity occurred when wind shear increase as given in the WRF standard-release and the 20 % decreasing scenario. Different situation is found in the 20 % increasing scenario where wind shear tend to weak and lead to strong wind intensity at this stage. Meanwhile, the reduction of moisture supply at 850-700 hPa resulting from 20 % decreasing relative humidity play a major role in the weakening of wind intensity and reduces mechanical energy of the cycle process as much as 300 J kg-1.
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