Judul: Erythrocyte Profile of Ongole Crossbred Cattle which Stored for 48 Hours in Heparin Anticoagulant
Abstrak: Erythrocyte is one of the main parameters in blood tests. The blood sample that tested is sometimes damaged, so it is necessary to add an anticoagulant to keep the blood intact. This study was conducted to determine the profile of erythrocytes in blood samples of Ongole Crossbred Cattle with heparin added and different storage times. This study used 9 Ongole Crossbred Cattle aged 4-5 years. Blood samples were collected through the coccygeal vein and stored in a vacutainer tube containing the anticoagulant heparin. Samples were stored in the refrigerator and analyzed for the total parameters of RBC, HGB, HCT, MCV, MCH, and MCHC at 0-3, 3, 6, 9, 12, 24, and 48 hours. The results showed that the total number of RBC, HGB, HCT, MCV, MCH and MCHC fluctuated during the observation time. The results of the examination of the total number of RBC, HGB, HCT, MCV, MCH and MCHC values of PO cattle added with heparin anticoagulant did not show significantly different results (P> 0.05) in each hour of observation. This shows that the addition of the anticoagulant heparin to Ongole Croosbred Cattle blood samples can be used for up to 48 hours., Eritrosit merupakan salah satu parameter utama dalam pemeriksaan darah. Sampel darah yang diuji terkadang mengalami kerusakan sehingga perlu ditambahkan antikoagulan agar kondisi darah tetap utuh. Penelitian ini dilakukan untuk mengetahui profil eritrosit Sapi Peranakan Ongole (PO) yang ditambahkan heparin dengan waktu penyimpanan berbeda. Penelitian ini menggunakan 9 ekor sapi PO yang berumur 4-5 tahun. Sampel darah dikoleksi melalui vena coccygea dan disimpan dalam tabung vaccutainer berisi antikoagulan heparin. Sampel disimpan dalam lemari pendingin dan dilakukan analisa terhadap parameter total RBC, HGB, HCT, MCV, MCH, dan MCHC pada waktu 0-3, 3, 6, 9, 12, 24, dan 48 jam. Hasil penelitian menunjukkan bahwa jumlah total RBC, HGB, HCT, MCV, MCH dan MCHC mengalami fluktuasi selama waktu pengamatan. Hasil pemeriksaan jumlah total nilai RBC, HGB, HCT, MCV, MCH dan MCHC sapi PO yang ditambahkan antikoagulan heparin tidak menunjukkan perbedaan yang nyata (P>0,05) dalam setiap jam pengamatan. Hal ini menunjukkan bahwa penambahan antikoagualan heparin pada sampel darah sapi PO dapat digunakan hingga rentang waktu sampai 48 jam.
Keyword: Heparin, HGB, HCT, MCV, MCH, MCHC, Ongole Croosbred Cattle, RBC

Judul: Observation of the Blood Leukocytes of  Ongole Crossbred Cattle Given with Ethylene Diamine Tetraacetic Acid Anticoagulants and Stored for 48 Hours
Abstrak: Complete blood count (CBC) merupakan salah satu pemeriksaan darah yang dilakukan untuk mengevaluasi kesehatan ternak. Beberapa faktor pra-analitik yang turut berpengaruh terhadap data yang dihasilkan di antaranya jenis antikoagulan yang digunakan, jeda waktu pemeriksaan, serta suhu penyimpanan. Oleh karena itu faktor tersebut penting dan perlu diperhatikan pada saat sebelum pemeriksaan darah dilakukan. Penelitian ini bertujuan untuk mengetahui jumlah dan morfologi leukosit darah yang diberi antikoagulan Tripotassium Ethylene Diamine Tetraacetic Acid (K3EDTA) dan disimpan selama 48 jam pada suhu 4 °C. Hewan yang digunakan adalah 10 ekor sapi peranakan ongole (PO) betina dengan umur 4 - 5 tahun. Sampel darah diambil melalui vena jugularis untuk diamati jumlah dan morfologi jenis sel leukosit dengan waktu penyimpanan 0, 3, 6, 9, 12, 24, dan 48 jam. Hasil penelitian menunjukkan jumlah total leukosit, limfosit, neutrofil, eosinofil, dan basofil sampai pengamatan 48 jam tidak menunjukkan perubahan nilai secara nyata (P>0,05) serta masih berada dalam kisaran normal. Jumlah monosit cenderung mengalami kenaikan dan secara statistik berbeda nyata pada penyimpanan 48 (P<0,05). Morfometri jenis sel leukosit mengalami pembesaran diameter sebanding dengan semakin lama penyimpanan. Selain perubahan ukuran sel, juga ditemukan vakuolisasi inti dan sitoplasma pada monosit, neutrofil, dan eosinofil mulai 24 jam penyimpanan., Complete blood count is one of the blood tests carried out to evaluate the health of livestock. Some pre-analytical factors that also affect the data prodeced include the type of anticoagulant used, the time lag of the examination, and the storage temperature. Therefore, the factor is important and needs to be considered before the blood examination is done. This study aimed to determine the number and morphology of blood leukocytes given the Anticoagulant Tripotassium Ethylene Diamine Tetraacetic Acid (K3EDTA) and stored for 48 hours at 4 °C. This study were used 10 female ongole crossbred cattle (PO) cows aged 4 - 5 years. Blood samples were collected through the jugular vein to observe the number and morphology of leukocyte cell types with storage times of 0, 3, 6, 9, 12, 24, and 48 hours. The results showed that the total number of leukocytes, lymphocytes, neutrophils, eosinophils, and basophils until 48-hour observation did not show a noticeable change in values (P>0,05) and was still within the normal range. The number of monocytes increase significantly at 48 hours of storage (P<0,05). Morphometry of leukocyte cell types undergoes a magnification in diameter proportional to the longer the storage. In addition to change in cell size, vacuolization of nucleus and cytoplasm were also found in monocytes, neutrophils, and eosinophils after being stored for 24 hours.
Keyword: Darah, K3EDTA, Leukosit, Morfometri, Sapi Peranakan Ongole

Judul: Perencanaan arsitektur enterprise di perguruan tinggi: Studi Kasus STMIK Darmajaya
Abstrak: The research is about enterprise architecture planning for university using Zachman framework. The research based on case study at STMIK Darmajaya-located at Bandar Lampung. research scope is limited to academic/education aspect as STMIK Darmajaya core business and one of three Tridarma Perguruan Tinggi. Academic aspect in STMIK Darmajaya consist of Three main actibity: student admission, academic operation and student graduation. Research output is enterprise information system blueprint according research scope above
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