Judul: Aplikasi Hormon Pertumbuhan Rekombinan melalui Perendaman untuk Memacu Pertumbuhan Benih Ikan Gurami (Osphronemus goramy)
Abstrak: This study aimed to determine the immersion frequency of recombinant common carp (CcGH) and giant gourami (OgGH) growth hormone in the growth stimulation of giant gourami juvenile. A total of 50 larvae at second day after first feeding, were immersed in 30 ppt salt water for 2 minutes, then transferred into 200 mL of 9 ppt saline water containing 30 mg/L CcGH in inclusion bodies form. Immersion was performed for 60 minutes with a frequency of 2, 3, 4 and 5 times at interval 7 days. The results showed that the highest (p<0.05) average body weight of giant gourami juvenile (2.232±0.190 g) was obtained at 4 times immersion treatment. The total dosage of 4 times immersion (120 mg/L OgGH) from the first experiment was then used in the second experiment to determine whether immersion frequency could be minimized. Fish larvae were immersed once a week, twice a week, and once a week for 2 weeks. The results showed that the highest (p<0.05) biomass (5482.58±110.70 g), and survival rate (87.38±8.84%) were obtained by once immersion time. Hence, both CcGH and OgGH could improve growth of giant gourami juvenile. The IGF-I mRNA expression level was increased significantly at 24 hours after OgGH immersion, and this suggested that IGF-I was involved in growth stimulation. Lower plasma cortisol levels in OgGH-treated fish after 180 minutes may also plays important role in growth induction. Thus, immersion of juvenile once a week in 120 mg/L OgGH solution can be applied to enhance the growth of giant gourami with a high survival rate.
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Judul: Kinerja Pertumbuhan Benih Ikan Gurame yang Diberi Perendaman Hormon Pertumbuhan Rekombinan dengan Masa Pemeliharaan Berbeda di Akuarium.
Abstrak: Slow growth of giant gourami became the main problem in cultivation of this commodity. Long rearing period surely would increase the risk and cost of production. Therefore, a technology is required to enhance growth rate of giant gourami. The aims of this experiment were to analyse growth performance of giant gourami to the recombinant giant gourami growth hormone (rOgGH) immersion at a dose of 120 mg/L for 60 minutes at juvenile stage and to evaluate the proper aquarium-rearing period. After immersed, fish were reared in aquarium for 2 weeks, 3 weeks and 4 weeks before moved to fish pond (aquarium-rearing period became the second factor on this experiment, in term to design a proper method to apply this technology). There were 3 replications for every treatment. RT-PCR analysis showed that IGF-I mRNA expression level increased just after rOgGH immersion about 208,3% compared with control. However, lipase analysis showed no differences between treated fish and control fish that is 0,055 unit/minute.g body weight and 0,053 unit/minute.g body weight for each group. Furthermore, treatment of rOgGH also altered the utilization of dietary and stored lipid, protein and carbohydrates. The results showed significant differences (P<0,05) for nutrient retention between treated fish and control fish. Fish that immersed by rOgGH had higher fat retention (15,92±0,37%) if compared with the control BSA fish (6,52±0,38%). Immersion treatment with rOgGH also had a higher protein retention that was 23,58±2,33% compared with BSA control fish 19,75±1,00%. Immersion treatment with rOgGH had a lower carbohydrate retention that was 2,86±0,21% if compared with BSA control 10,63±0,92%. Observation to feed efficiency value showed that immersion in rOgGH had no statistically significant affect (P>0,05) compared with BSA control for 3 types of feed. For tubifex worms, the feed efficiency value that reached was 14,8±5,6% (rOgGH) and 12,7±5,0% (BSA). So that crumble feed, 51,6±17,8% (rOgGH) and 41,9±13,6% (BSA), for small feed (2mm) 66,8±9,0% (rOgGH) and 54,8±10,0% (BSA). Treatment that gave a significant affect was aquarium-rearing period, it showed that fish which reared in aquarium for 2 weeks had a higher feed efficiency value for tubivex worm and crumble feed that were ,7±2,8% and 60,5±7% if compared with fish which reared in aquarium for 3 and 4 weeks with efficiency value for each were 15,6±2,1% and 7,9±4,4% for tubifex worms and 30,7±5,0% and 49,0±16,5% for crumble feed. Growth acceleration were expected in this immersion treatment, but results showed that there were no differences between rOgGH treated fish and BSA control fish (8,7±0,3% and 8,4±0,3%). Aquarium-rearing period seemed to be the effecting-factor which gave significant affect (P<0,05) to fish growth, it showed that fish that reared in aquarium for 2 weeks had a faster growth that was 8,7±0,2% if compared with fish that reared in aquarium for 3 and 4 weeks (8,3±0,1% and 8,6±0,5% for each group). Results also showed that immersion in rOgGH had no significant affect (P>0,05) if compared with BSA control for survival rate (58,9±17,9% and 57,9±14,6% for each group). Aquarium-rearing period seemed to have significant affect (P<0,05) for survival rate which fish that reared in aquarium for 2 weeks had a higher survival
Keyword: recombinant growth hormone, immersion, rearing period, growth, metabolic pathways, giant gourami

Judul: Penentuan Nilai Eigen dan Vektor Eigen dari Matriks Tridiagonal 2-Toeplitz dengan Pendekatan Polinomial Chebyshev
Abstrak: The eigenvalues and eigenvectors of a matrix can be determined by finding its characteristic polynomials. The characteristic polynomials of a tridiagonal 2-Toeplitz matrix is shown to be closely connected to polynomials which satisfy the Chebyshev recurrence relationship. If the order of the matrix is odd, then the eigenvalues are found explicitly in terms of the Chebyshev zeros and the eigenvectors are found in terms of the polynomials satisfying the recurrence relationship. For even ordered matrices, the situation is more complicated. The problem in these cases is that although the Chebyshev recurrence formula is still applied, its initial values are not generating Chebyshev polynomials
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