Judul: Analisis ekspresi gen penyandi enzim antioksidan pada kulit batang hevea
Abstrak: Tingginya permintaan dunia akan karet perlu diimbangi dengan usaha untuk meningkatkan produksi lateks di antaranya melalui penggunaan stimulan seperti etefon. Stimulasi etefon yang berlebiian oleh tanaman kmt difespons sebagai cekaman bagi kehidupannya sehingga menyebabkan akumulaqi Reactive Oxygen Species (ROS) yang memicu kematian sel. Penelitian ini bertujuan mempelajari ekspresi gen penyandi enzim antioksidan yang terlibat dalam ROS-Scavenging system, yang meliputi mangan superoksida dismutase (MnSOD), askorbat peroksidase (APX), Zn-Cu superoksida dismutase (Zn-CuSOD), glioksilatkarboligase kloroplastik (GCLchl), katalase (CAT), dan glikosilatkarboligase sitosolik (GCLcyt) terhadap pengamh pelukaan dan pemberian etilen menggunakan aktin sebagai kontrol. RNA karet dari kulit batang diisolasi kemudian dikarakterisasi menggunakan spektrofotometer dan elektroforesis gel agarosa. Ekspresi gen diamati menggunakan rnetode RT-PCR.Ekspresi gen CuZnsod, gclsitosolik, Mnsod, gclkloroplastik, cat, dan Hbapx umumnya meningkat setelah dilakukan penyadapan sebanyak tujuh kali. Peningkatan ekspresi gen tertinggi diperlihatkan oleh gen Mnsod sebesar 13,38. Ekspresi gen menurut setelah diberi perlakuan penyadapan 4 hari sekali dan pemberian etilen 12 kali dalam setahun yang menunjukkan bahwa tanaman tersebut mengalami cekaman oksidatif karena kehadim ROS. Hal ini memberi peluang sebagai penanda molekuler dalam seleksi klon-klon karet berdaya hasil tinggi dan responsif terhadap aplikasi etefon.
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Judul: Expression Gene of ACC Oksidase effect of Etilena Eksogen and Tapping at Hevea brasiliensis Clone of PB 260.
Abstrak: High request of natural rubber on the world have made researcher to do increase natural rubber product and also look for or yield good clones holding to disease and have high production. This research used clone PB 260 coming from result of cross of primary clone and secondary clone. This clone have fastly of high latex metabolism but less responsive to stimulan. Effort to increase natural rubber product with treatment of tapping and usage of etephon stimulan. Etephon is compound 2-chloro-ethylposphonic acid (CEPA) as incentive or stimulant to increase produce hormone of ethilene endogen at rubber crop. Gene of HbACO1 will be test its expression of etefon and influence of tapping at bark and latex of rubber crop. This research aim to study gene expression of HbACO1 to influence of tapping and giving stimulant at various phase growth of crop. Result of research show gene of HbACO1 only have expression at bark of rubber crop not in latex. Treatment which good to bark of rubber crop is 2 day once tapping and 3 times in one year giving stimulant.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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