Judul: Identifikasi keragaman DNA mikrosatelit lokus CSSM018, ILSTS054 dan IDVGA-30 pada domba lokal
Abstrak: The objectives of this research were (1) to determine genetic variation, (2) to analyze genetic differentiation and (3) genetic distance of local sheep. The blood samples were collected from 96 heads local sheep from 8 regions. Three pairs of primers were used to amplify three loci microsatellites DNA by Polymerase Chain Reaction (PCR) technique. The result showed that the alleles distribution were various for all breeds. CSSM018 locus was the most various allele for all breeds. d allele of CSSM018 locus was found on Madura Fat Tail sheep and a allele of IDVGA-30 locus was founded on Donggala and Madura Fat Tail sheep. Another hand, b allele of ILSTS054 was founded on Margawati Medium Tail Sheep. The highest frequency of allele was a allele of ILSTS054 locus for each breeds. They were 79,27%, 79,17% and 77,08%, respectively. The highest of heterozygosity (h) were 0,5607, 0,5405 and 0,3768 for CSSM018, IDVGA-30 and ILSTS054 loci, respectively. Average heterozygosity (H) for Fat Tail sheep, Medium Fat Tail Sheep and Thin Tail sheep were 0,4694, 0,4645 and 0,4686, respectively. The genetic deferentiation of Fat Tail sheep, Medium Tail sheep and Thin Tail sheep were 0,1497, 0,1235 dan 0,3858, respectively. Geneticly, the Thin Tail sheep was close to Medium Tail sheep (D = 0,7558), but Thin Tail sheep was far to Fat Tail sheep (D = 0,801 1). Keywords: Indonesian local sheep, Microsatellite, Genetic variation
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Judul: Identifikasi Keragaman Dna Mikrosatelit Lokus CSSM066, ILSTS029 Dan ILSTS061 Pada Sapi Katingan Di Kalimantan Tengah
Abstrak: The objective of this study was to identify the polymorphism of CSSM066, ILSTS029 and ILSTS061 microsatellite DNA loci on Katingan cattle in Central Kalimantan. The blood samples used in this study was 70 samples originated from the three sub populations of Pendahara sub populations (26 samples), Buntut Bali (13 samples) and Tumbang Lahang (31 samples). DNA amplification was done by using Polymerase Chain Reaction (PCR) then electrophoresed using 6% polyacrylamide gel followed by silver staining. Data were analyzed using allele frequency, genotype frequency, degree of heterozygosity and genetic tree. The result showed that CSSM066, ILSTS029 and ILSTS061 microsatellite DNA loci were high polymorphisms. CSSM066 locus had 9 alleles with a length of 168-188 bp allele. The highest allele frequency was allele G (0.385) in Buntut Bali sub populations and the lowest allele O (0.017) in Tumbang Lahang sub populations. ILSTS029 locus had 16 alleles with a length of 159-180 bp allele. The highest allele frequency was allele L (0.333) in Buntut Bali sub populations and lowest allele H, P and V (0.017) in Tumbang Lahang sub populations. ILSTS061 locus had 14 alleles with a length of 136-164 bp allele. The highest allele frequency was allele G (0.308) in Buntut Bali sub populations and the lowest is allele G and I (0.021) in Pendahara sub populations. The highest heterozygosity values was found in Tumbang Lahang sub population (ĥ=0.7333) ILSTS029 locus and lowest in Pendahara sub population (ĥ=0.0000) for CSSM066 locus. The highest heterozigosity average was found (Ĥ=0.6558) in ILSTS029 locus and the lowest (Ĥ=0.1692) in CSSM066 locus.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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