Judul: Molecular identification of the indian mackerel (Rastrelliger  kanagurta) based on 16S rRNA gene markers in FMA NRI 572 and 573 as a basis  for fisheries management
Abstrak: Ikan kembung lelaki (Rastrelliger kanagurta) memiliki nilai ekonomis penting bagi nelayan. Ikan pelagis kecil di WPP-NRI 572 dan WPP-NRI 573 telah mengalami kondisi fully-exploited. Penelitian ini bertujuan memastikan spesies, keragaman genetik dan kekerabatan spesies secara molekuler ikan kembung lelaki (Rastrelliger kanagurta) berdasarkan marka gen 16S rRNA di WPP-NRI 572 dan 573 sebagai dasar pengelolaan perikanan. Penelitian dilakukan pada bulan Juni 2021 hingga Januari 2022. Sampel ikan diambil dari perairan Labuan, Cilacap, dan Banyuwangi dengan jumlah total sebanyak 9 individu. Urutan basa nukleotida hasil sekuensing dianalisis menggunakan software MEGA X. Hasil identifikasi DNA barcoding, menunjukkan spesies ikan kembung dari tiga lokasi berbeda merupakan ikan kembung lelaki (Rastrelliger kanagurta). Nilai keragaman genetik ikan kembung lelaki dari tiga lokasi termasuk dalam kategori tinggi. Hal ini menunjukkan populasi ikan kembung tergolong stok yang baik, The Indian Mackerel has important economic value for fishermen. The small pelagic fisheries in WPP-NRI 572 and WPP-NRI 573 have experienced a fully-exploited condition. This study aims to determine species certainty, genetic diversity and genetic relationships of indian mackerel (Rastrelliger kanagurta) molecularly based on 16S rRNA gene markers in WPP-NRI 572 and 573 as a basis for fisheries management. The research was conducted from June 2021 to January 2022. Fish samples taken from the waters of Labuan, Cilacap, and Banyuwangi with a total of 9 individuals. The nucleotide base sequences of the sequencing results were analyzed using MEGA X software. The identification results of DNA barcoding showed that the mackerel species studied from three different locations were the Indian Mackerel (Rastrelliger kanagurta). The results showed that the genetic diversity value of male mackerel from three locations was included in the high category indicating that the mackerel population was classified as a good stock.
Keyword: 16S rRNA genes, genetic diversity, Rastrelliger kanagurta

Judul: Molecular identification of the indian mackerel (Rastrelliger kanagurta) based on COI gene markers in Sunda Strait, Cilacap, and Banyuwangi in supporting fisheries management.
Abstrak: Ikan kembung lelaki (Rastrelliger kanagurta) memiliki nilai ekonomis penting bagi nelayan. Ikan pelagis kecil di WPPNRI 572 dan WPPNRI 573 telah mengalami kondisi fully-exploited. Tekanan eksploitasi yang tinggi akan menurunkan keragaman genetik spesies. Penelitian ini bertujuan untuk memastikan spesies, menganalisis kekerabatan dan keragaman genetik secara molekuler ikan kembung lelaki dengan menggunakan marka gen COI di Perairan Selat Sunda, Cilacap dan Banyuwangi. Isolasi dan ekstraksi DNA menghasilkan 30 DNA total, lalu diamplifikasi dengan metode PCR. Hasil sekuensing divalidasi dengan fitur BLASTn pada aplikasi MEGA 11, menunjukkan bahwa 30 contoh dari ke tiga lokasi merupakan spesies R. kanagurta dengan kemiripan antara 98,15% hingga 100%. Jarak genetik ikan kembung lelaki di tiga lokasi memiliki nilai yang rendah. Hasil pohon filogeni menunjukkan contoh memiliki hubungan kekerabatan yang dekat dan adanya pencampuran populasi dari tiga lokasi, sehingga dapat dikelola dalam satu unit pengelolaan., The Indian Mackerel has important economic value for fishermen. The small pelagic fisheries in WPPNRI 572 and WPPNRI 573 have experienced a fully-exploited condition. High exploitation pressure will reduce the genetic diversity of Indian Mackerel species in the wild. This study aims to determine the species, analyze the molecular kinship and genetic diversity of Indian Mackerel (Rastrelliger kanagurta) using COI gene markers in Sunda Strait waters, Cilacap and Banyuwangi. DNA isolation and extraction yielded 30 total DNA, amplified by the PCR method. Sequencing results were validated with the BLASTn feature on the MEGA 11 application, showing that 30 samples were R. kanagurta species with the highest similarity of 100%. The genetic distance of Indian Mackerel in three locations has a low value. The results of the phylogeny tree show that the sample has a close relationship, and there is a mixing of populations from three locations so that it can be managed in one management unit.
Keyword: COI, genetic, Rastrelliger kanagurta

Judul: Pembuatan Film Tipis Cu2ZnSnS4 sebagai Lapisan Penyerap Cahaya pada Sel Surya Menggunakan Metode Elektrodeposisi
Abstrak: Thin film of Cu2ZnSnS4 (CZTS) is an alternative semiconductor used as light absorber layer on solar cell. CZTS can replace silicon that commonly used as commercial semiconductor. The advantage of using CZTS as absorber layer is due to the abundance of its chemical composer on earth, and its high value of absorption coefficient. The absorber layer was made by using electrodeposition method on ITO glass substrates. The films were deposited at room temperature using potentiostatic mode with a potential of –1.05 V for 30, 45, and 60 minutes. After that, the films were annealed at two different temperatures, 180 ºC and 500 °C, for 45 minutes in N2/H2S atmosphere. Based on X-ray diffraction analysis, the polycrystalline thin film obtained fall into kesterite crystal phase. This type of crystal was obtained when the CZTS was annealed at 500 °C in H2S/N2 atmosphere. The value of optical band gap energy for the CZTS thin film was 2.2 eV with the film thickness about 1.228–6.366 μm. The sample of CZTS with electrodeposition time of 45 minutes and annealing temperature of 500 °C showed the best result and in conformity with absorber layers criteria for solar cells.
Keyword: semiconductor, ITO glass, CZTS thin film, electrodeposition