Judul: Research of Internet Addiction for the Design of Finet Community  features Application
Abstrak: Perkembangan internet secara signifikan meningkatkan kecenderungan meningkatnya adiksi internet, terutama yang dijumpai pada kalangan muda atau yang didefinisikan sebagai dewasa muda. Permasalahan pada penelitian ini adalah minimnya informasi mengenai kondisi orang dengan adiksi internet pada kelompok usia tersebut. Salah satu cara untuk mengatasi adiksi internet adalah menggunakan teknik support groups. Penelitian ini menggunakan metode User Centered Design dengan tujuan untuk mengetahui fokus permasalahan sehingga dapat menghadirkan pengalaman yang disesuaikan untuk orang dengan adiksi internet dengan fokus pada kelompok usia dewasa muda usia 18-29 tahun dengan melibatkan bantuan dukungan dari support groups yang diimplementasikan dalam fitur komunitas aplikasi Finet. Dalam penelitian ini hanya berfokus pada tahapan research users dan assess the situation, sedangkan tahapan lainnya dilakukan oleh saudara Rafiandi Ammar Putra. Hasil akhir penelitian ini disajikan dalam bentuk user persona, user journey maps, dan assess the situation checklist., The development of the internet has significantly increased the progress of internet addiction, which is found among young people or defined as young adults. The problem in this study is the lack of information about the condition of people with internet addiction in this age group. One way to overcome internet addiction is to use support group techniques. This study is using User Centered Design method to determine the focus of the problem so that it can present a tailored experience for people with internet addiction with a focus on the young adult age group aged 18-29 years by involving support from support groups that are implemented in the community feature of the Finet application. This study only focuses on the research user assess the situation step, while the other steps will be done by Rafiandi Ammar Putra. The result of this study is presented in the form of user persona, user journey maps, and assess the situation checklist.
Keyword: community, internet addiction, research, support groups, user centered design

Judul: Addiction of Internet Usage (Social Networking Sites) Among Digital Native on South Tangerang Commuter Train Line
Abstrak: Penggunaan internet telah mengubah gaya hidup masyarakat pada dua dekade terakhir. Aspek yang berubah meliputi, cara berkomunikasi, bersosialisasi, hiburan, pekerjaan, hingga pola berfikir. Banyak dampak positif yang berkaitan dengan penggunaan media sosial, seperti menjalin hubungan, tetapi ada juga dampak negatif yang ditimbulkan. Cara pengumpulan data dilakukan dengan pendekatan kuantitatif dan kualitatif dengan total 153 responden. Setiap partisipan mengisi kuisioner yang berisi tes adiksi internet bersamaan dengan pertanyaan mengenai pemakaian internet dalam satu hari. Hasil dari penelitian ini menunjukkan bahwa responden yang lebih muda telah mengenal internet sejak dini dibandingkan dengan responden yang lebih tua. Sebesar 52.29% responden menggunakan internet lebih dari enam jam per hari. Rata-rata nilai adiksi internet sebesar 52 poin untuk pria dan 48.7 poin untuk wanita. Nilai adiksi internet menurun sepanjang pertambahan umur (p-value= 0.005). Nilai adiksi internet meningkat sepanjang durasi penggunaan internet per hari (p-value= 0.006). Media sosial yang paling banyak digunakan antara lain, Instagram, Line, Whatsapp, Youtube, Facebook, dan permainan. Penelitian ini menemukan adanya hubungan antara penggunaan internet dan adiksi pada masyarakat digital. Hasil dari penelitian ini juga menunjukkan bahwa pengguna yang lebih muda memiliki tingkat adiksi yang lebih tinggi dibanding responden yang lebih tua.
Keyword: adiksi, masyarakat digital, media sosial

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable