Judul: Pengaruh Pemberian Ekstrak Minyak Jinten Hitam (Nigella sativa) Terhadap Gambaran Mikroskopis Paruparu Mencit (Mus musculus)
Abstrak: This study was aimed to get information about the effect of blackseed (Nigella sativa) effect on the histopathology of mice lung. Twelve male mice of 4 weeks old were divided into four groups, each group consisted of three mice. Group K or negative control received aquadest, group HS 0.1 was received 0.1 ml blackseed oil, group HS 0.2 was received 0.2 blackseed oil and group HS Madu was received 0.3 ml mixed of blackseed oil and honey (1:20) for two months. At the end of the experiment, the mice were euthanized by atlanto occipital dislocation and necropsied to collect the lung sample. The lungs were processed to prepare the histopathology slides with Hematoxyllin-Eosin and Periodic Acid Schiff (PAS) stain. The parameters observed include counting percentage of bronchus and bronchial presenting exudates, counting the goblet cell between the respiratory epithelial cell, calculating the BALT area, counting the limfoid cell density of the BALT, counting the congestion, hemorrhage, inflammation and emphysema area on the certain fields of view, measuring the thickness of smooth muscle surround bronchial using Image J® for Microsoft® Windows®. Quantitative data were analyzed using SPSS® 16.0 software and ANOVA test followed Duncan test. The result showed that Nigella sativa treatment does not lead to increase exudation in the bronchus and bronchioles lumen,a decrease in the activity of goblet cells, a decrease the BALT area and an increase in the lymphoid cell density,a reduce the area of congestion and hemorrhage, inflammation and emphysema, reduction at the smooth muscle thickness. The blackseed content such thymoquinone and longifolene were thought to provide a favorable effect on lung histomorphology.
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Judul: Pengaruh Pemberian Ekstrak Minyak Jintan Hitam (Nigella sativa) terhadap Gambaran Diferensiasi Leukosit dan Luasan Sumsum Tulang Mencit (Mus musculus)
Abstrak: This study was aimed to get information about blackseed (Nigella sativa) suplementation effect on leukocyte differentiation and bone marrow of mice. Seventy two mice (36 male and 36 female) of 4 weeks old were divided into four group, each group consists of nine mice. Group I was negative control (received aquadest 0.1 ml), group II (received 0.1 ml blackseed oil), group III (received 0.2 ml blackseed oil), and group IV (received 0.3 ml combination of blackseed oil and honey). This treatment were done for two months. Afterward, the mice were euthanized and then necropsied followed by blood and os femur collection as the sample. The blood samples were processed to prepare blood smear and os femur samples were processed to prepare histopathology slides with Hematoxilyn-Eosin stain. The parameters observed include to count and differenciate of the leukocyte cells and bone marrow volume using Image J® software for Microsoft® Windows®. Quantitative data were analyzed with SAS® 9.1.3 software for Microsoft® Windows®, ANOVA test and followed by Duncan test. The result showed that blackseed oil caused increasing of lymphocyte and bone marrow volume, and decreasing of neutrophyl on group II, III, and IV which were significant (p<0.05) with the control group
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Judul: Cycle Hamilton pada Graf Lengkap, Graf Regular, dan Graf 2-Connected 4-Regular Berorder Kurang dari Sepuluh
Abstrak: Suatu graf disebut graf Hamilton apabila graf tersebut memuat cycle Hamilton, yaitu cycle yang melewati setiap vertex tepat satu kali. Menentukan keberadaan cycle Hamilton pada suatu graf dapat menggunakan syarat cukup yang berupa teorema-teorema yang telah diperkenalkan sejak tahun 1950-an. Beberapa syarat cukup yang dibahas dalam karya ilmiah ini adalah syarat cukup yang berhubungan dengan graf lengkap dan graf regular. Suatu graf lengkap berorder 𝑛������ ≥ 3 dan graf r-regular berorder 𝑛������ ≤ 2𝑟������ + 1 merupakan graf Hamilton, Karena kedua graf tersebut memuat cycle Hamilton. Selanjutnya, syarat cukup tersebut digunakan untuk menentukan keberadaan cycle Hamilton pada graf 2-connected 4- regular berorder kurang dari 10.
Keyword: graf Hamilton, graf regular, graf lengkap, graf 2-connected 4-regular