Judul: Studi Infestasi Caplak dan Deteksi Borrelia spp. pada Caplak yang Menginfestasi Kura-Kura Darat
Abstrak: Hard-bodied ticks of the genus Amblyomma that parasitize reptiles are potential vectors of zoonoses. This study aimed to describe tick infestations and detect Borrelia spp. infections in ticks found on tortoises. One hundred six tortoise hosts were examined at selected locations in Indonesia. DNA extraction and Borrelia spp. detection were carried out by conventional PCR. In this study, nineteen hard-bodied ticks were collected from one Chelonoidis carbonarius, one Chelonoidis denticulatus, and six Geochelone sulcata. All collected ticks were identified as Amblyomma sparsum. The prevalence of A. sparsum on tortoises was 7.55%. Borrelia spp. were detected in several of the A. sparsum infested on tortoises. Sequencing revealed two separate reptile-associated (REP) Borrelia strains., Caplak keras dari genus Amblyomma yang menjadi parasit pada reptil berpotensi menjadi vektor zoonosis. Penelitian ini bertujuan mendeskripsi infestasi caplak dan mendeteksi infeksi Borrelia spp. pada caplak yang ditemukan pada kura-kura darat. Seratus enam inang kura-kura darat diperiksa di beberapa tempat di Indonesia. Ekstraksi DNA dan deteksi Borrelia spp. dilakukan menggunakan metode PCR konvensional. Hasil penelitian diperoleh sembilan belas caplak keras yang dikoleksi dari seekor kura-kura darat jenis Chelonoidis carbonarius, seekor Chelonoidis denticulatus, dan enam ekor Geochelone sulcata. Jenis caplak yang ditemukan adalah Amblyomma sparsum, dengan prevalensi sebesar 7.55%. Borrelia spp. dideteksi pada beberapa A. sparsum yang menginfestasi kura-kura darat. Hasil sekuensing menunjukkan adanya dua galur reptile-associated (REP) Borrelia.
Keyword: Amblyomma sparsum, PCR, reptile-associated Borrelia, tick infestations, tortoises

Judul: Detection of Anaplasma spp., Rickettsia spp., and Borrelia spp., on Brown Dog Ticks (Rhipicephalus sanguineus) in Bogor
Abstrak: Caplak keras Rhipicephalus sanguineus merupakan ektoparasit yang biasa menginfestasi anjing dan berpotensi menjadi vektor zoonosis. Penelitian bertujuan mendeteksi jenis bakteri patogen yaitu Anaplasma spp., Rickettsia spp., dan Borrelia spp. dari caplak yang dikoleksi di Bogor. Sebanyak 77 ekor anjing diperiksa secara acak dari total 217 anjing dan ditemukan 142 caplak hasil koleksi dari enam ekor anjing. Sampel caplak dilakukan ekstraksi DNA untuk mendeteksi bakteri patogen dengan menggunakan metode PCR. Hasil PCR terhadap gen 17-kDa dan gltA menunjukkan satu sampel caplak positif terdapat Rickettsia sp. yang mendekati Rickettsia felis pada caplak yang menginfestasi anjing di Saint Kitts and Nevis. Hasil PCR terhadap gen GroEL menunjukkan satu hasil positif Anaplasma sp. yang mendekati Anaplasma platys pada caplak di China yang menginfestasi anjing sedangkan deteksi Borrelia spp. menunjukkan hasil negatif pada setiap sampel caplak.
Keyword: Anaplasma, Anjing, Borrelia, Rhipicephalus sanguineus, Rickettsia

Judul: Approximation of the Median of Poisson Distribution Using the Relation of Poisson-Gamma Distribution
Abstrak: A major drawback of median is that median cannot usually be defined in closed form, even in the cases where their distribution functions are known. This study gives an approximation of the closed form for the median of the Poisson distribution by using the relation of Poisson-Gamma distribution. The approximation uses elementary techniques based on the monotonicity of certain sequences involving tail probabilities of the Poisson distribution and the Central Limit Theorem. Furthermore, a closed form expression for the mean absolute deviation 􀜧|􀜰􀰒 􀵆 􀜯􀝁􀝀􁈺􀜰􀰒􁈻| is also studied. The median is relevant because of the fact that it minimizes the mean absolute deviation of a Poisson random variable (􀜰􀰒􁈻 by considering median as central tendency.
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