Judul: The Characterization of Microbiome Through Next Generation Sequencing (NGS) Platform on Indo-Pasific Bottlenose Dolphin (Tursiops aduncus)
Abstrak: Mamalia laut merupakan memainkan peran penting dalam ekosistem laut, sebagai predator puncak, konsumen primer dan sekunder, serta sebagai indikator kesehatan ekosistem laut. Salah satu mamalia laut yang banyak ditemukan di Indonesia adalah lumba-lumba hidung botol Indo-Pasifik (Tursiops aduncus). Terlepas dari kepentingannya, terdapat beberapa hal yang menyebabkan terjadinya penurunan populasi lumba-lumba, salah satunya adalah infeksi penyakit yang disebabkan oleh mikroba patogen termasuk didalamnya virus, bakteri, dan jamur. Saat ini informasi mengenai mikrobioma pada mamalia laut khususnya lumba-lumba hidung botol Indo-Pasifik (T.aduncus) masih sangat terbatas, terutama informasi mengenai mikrobioma jamur. Penelitian ini bertujuan untuk mengeksplorasi profil mikrobima jamur pada lumba-lumba hidung botol Indo-Pasifik (T.aduncus) yang diambil pada saluran pencernaan lumba-lumba (lambung dan feses) dengan menggunakan platform Next Generation Sequencing (NGS). Hasil penelitian menunjukan Filum Ascomycota (12.233%, 85.46%) dan Basidiomycota (1.584%,11.06%) memiliki kelimpahan tertinggi pada saluran pencernaan lumba-lumba, hanya sedikit ditemukan filum Mortierellomycota (0.309%, 2.16%) dan Mucoromycota (0.188%, 1.31%). Hasil analisis Internal Transcribed Spacer (ITS) metagenomik, ditemukan kelimpahan relatif mikrobioma jamur pada lambung dan feses lumba-lumba hidung botol Indo-Pasifik (T.aduncus) didominasi oleh genus Aspergillus, diikuti Alternaria dan Malassezia. Aspergillus dominan terdapat pada sampel lambung, sedangkan Alternaria dan Malassezia lebih dominan terdapat pada sampel feses., Marine mammals play an important role in marine ecosystems as apex predators, primary and secondary consumers, and as indicators of the health of marine ecosystems. One of Indonesia's most common marine mammals is the Indo-Pacific bottlenose dolphin (Tursiops aduncus). Regardless of its importance, several things cause a decline in the dolphin population, such as infectious diseases caused by pathogenic microbes, including viruses, bacteria, and fungi. Currently, information about the microbiome of marine mammals, especially the Indo-Pacific bottlenose dolphin (T.aduncus) are still limited, especially information about the fungal microbiome. This study aimed to explore the profile of the fungal microbiome in the Indo-Pacific bottlenose dolphin (T.aduncus) taken from the digestive tract of dolphins (stomach and feces) using the Next Generation Sequencing (NGS) platform. The results showed that the phylum Ascomycota (12.233%, 85.46%) and Basidiomycota (1.584%, 11.06%) had the highest abundance in the digestive tract of dolphins. Only a few were found phylum Mortierellomycota (0.309%, 2.16%) and Mucoromycota (0.188%, 1.31). %). The metagenomic Internal Transcribed Spacer (ITS) analysis found the relative abundance of the fungal microbiome in the stomach and feces of the Indo-Pacific bottlenose dolphin (T.aduncus) was dominated by the genus Aspergillus, followed by Alternaria and Malassezia. Aspergillus was dominant in gastric samples, while Alternaria and Malassezia were dominant in stool samples.
Keyword: Marine Mammals, Microbiome, Next Generation Sequencing (NGS), Tursiops aduncus

Judul: Identifikasi Bakteri Asal Saluran Pernafasan Atas (Blowhole) Lumba-lumba Hidung Botol Indo-Pasifik (Tursiops aduncus) di Kawasan Konservasi Pantai Cahaya Kendal Jawa Tengah
Abstrak: Recently years, dolphin conservations more active to do in Indonesia. The population of dolphin has declined because of illegal hunting. The concerned problems of dolphin conservations are health management which related with infectious diseases. The knowledge of microorganisms associated with dolphin is still limited, particularly which is bacteria in upper respiratory tract. The aim of this research was to identify the type of bacteria in upper respiratory tract of indo-pacific bottlenose dolphin (Tursiops aduncus). Swab sampling from the upper respiratory tract (blowhole) of 11 dolphins was cultured in selective media. This research found 15 types of bacteria in the upper respiratory tract such as Actinobacillus sp., Pseudomonas sp., Moraxella sp., Bacillus sp., Staphylococcus aureus, Staphylococcus epidermidis, Proteus sp., Citrobacter sp., Salmonella sp., Serratia sp., Klebsiella sp., Pasteurella sp., Edwardsiella tarda, Alcaligenes faecalis, and Yersinia sp. The frequent bacteria that found in dolphin upper respiratory tract are Actinobacillus sp., Pseudomonas sp., Moraxella sp., and Bacillus sp. Some bacterias, such as Pseudomonas sp., Staphylococcus aureus., Staphylococcus epidermidis, Salmonellasp., Klebsiella sp., Proteus sp., Pasteurella sp., and Edwardsiella tarda are potentially patogen for dolphins.
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Judul: Pembuatan Film Tipis Cu2ZnSnS4 sebagai Lapisan Penyerap Cahaya pada Sel Surya Menggunakan Metode Elektrodeposisi
Abstrak: Thin film of Cu2ZnSnS4 (CZTS) is an alternative semiconductor used as light absorber layer on solar cell. CZTS can replace silicon that commonly used as commercial semiconductor. The advantage of using CZTS as absorber layer is due to the abundance of its chemical composer on earth, and its high value of absorption coefficient. The absorber layer was made by using electrodeposition method on ITO glass substrates. The films were deposited at room temperature using potentiostatic mode with a potential of –1.05 V for 30, 45, and 60 minutes. After that, the films were annealed at two different temperatures, 180 ºC and 500 °C, for 45 minutes in N2/H2S atmosphere. Based on X-ray diffraction analysis, the polycrystalline thin film obtained fall into kesterite crystal phase. This type of crystal was obtained when the CZTS was annealed at 500 °C in H2S/N2 atmosphere. The value of optical band gap energy for the CZTS thin film was 2.2 eV with the film thickness about 1.228–6.366 μm. The sample of CZTS with electrodeposition time of 45 minutes and annealing temperature of 500 °C showed the best result and in conformity with absorber layers criteria for solar cells.
Keyword: semiconductor, ITO glass, CZTS thin film, electrodeposition