Judul: Pendugaan Life Table Penduduk Wanita Indonesia Berdasarkan Data Sensus
Abstrak: Indonesian life tables are obtained indirectly using Coale-Demeny life table, because of the difficulty of getting data required especially the data of population mortalities. One of direct data set to be considered is the number of survival to age . In life insurance, life tables are used to determine the amount of premium to be paid by the insurance holder. This paper is intended to estimate a life table based on census data. This is done using surviving probability method, which will be done in three different approaches, i. e. smoothed Coale-Demeny life table, smoothed logit system, and the third method is the projection and accumulation approach. The resulting life tables are then compared to the life tables of BPS and concluded that both are similar.
Keyword: Coale-Demeny life table., surviving probability, life table

Judul: Pendugaan Life Table Penduduk Wanita Menggunakan Data Durasi Pernikahan dan Metode Berbasis Regresi
Abstrak: Data kematian merupakan salah satu data kependudukan yang banyak digunakan orang saat ini, seperti para pekerja kesehatan masyarakat, demografer, petugas asuransi dan ekonomi. Data kematian suatu negara biasanya dinyatakan dalam bentuk life table. Life table Indonesia saat ini dihitung menggunakan metode tidak langsung berdasarkan life table Coale-Demeny, dikarenakan sulit dalam mendapatkan sumber data yang dibutuhkan untuk membentuk life table. Penelitian ini bertujuan untuk menduga life table penduduk wanita dengan menggabungkan mortalitas anak perempuan berdasarkan data durasi pernikahan dan mortalitas perempuan dewasa dengan data proporsi ibu yang masih hidup menggunakan metode berbasis regresi. Data yang digunakan diperoleh dari Badan Pusat Statistika tahun 2010 dan dilengkapi dengan data bangkitan. Kelompok umur life table yang didapat belum lengkap dan dilengkapi dengan menggunakan laju kematian distribusi Gompertz dan Makeham. Hasil ekstrapolasi yang didapat dengan menggunakan laju kematian Gompertz dan Makeham menghasilkan angka harapan hidup berturut-turut adalah 50.81 dan 51.33, sedangkan angka harapan hidup wanita di Indonesia pada tahun 2010 adalah 71.83., Life table is one of the population data that is often used by people today, such as public health workers, demographers, insurance officers and economists. Data on mortality in a country are usually expressed in the form of a life table. Indonesia's life table is currently calculated using an indirect method based on the Coale-Demeny life table, because it is difficult to find the source of data needed to form a life table. This manuscript is intended to estimate the life table women population by combining child mortality based on data on duration of marriage and mortality of adult women using the proportion of mother was alive and regression-based methods. The data used were obtained from Badan Pusat Statistika (BPS) in 2010 and completed by generation data. The age group life table obtained was incomplete and was completed by using the Gompertz and Makeham distribution of mortality rates. The result of the probability of death obtained from life table using the Gompertz and Makeham mortality generate life expectancy 50.81 and 51.33, while the life expectancy of Indonesian women calculated by BPS in 2010 is 71.83.
Keyword: duration of the marriage, Gompertz, Makeham, life table, RegressionBased Method

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.