Judul: Keterandalan dan Intensitas Keruskan Konstruksi Bangunan Gedung Permukiman Di Jakarta Selatan
Abstrak: Jumlah penduduk berkaitan erat dengan peruntukan lahan bagi bangunan pemukiman. Proses kerusakan bangunan salah satunya disebabkan oleh faktor manusia. Manusia perlu pengetahuan tentang kondisi bangunan, faktor-faktor perusaknya dan cara perawatan sedini mungkin sehingga masa pakai bangunan bisa menjadi lebih lama. Penelitian ini bertujuan untuk mengetahui keterandalan dan intensitas kerusakan bangunan gedung permukiman di Jakarta Selatan. Penentuan bangunan rumah contoh dilakukan dengan teknik pengambilan contoh acak dengan empat tahap. Pada masing-masing rumah dilakukan pengamatan terhadap ada atau tidaknya kerusakan pada komponen bangunan rumah serta dilakukan wawancara dengan masing-masing penghuni rumah untuk menghimpun informasi umur bangunan, pemeliharaan dan perawatan bangunan yang kemudian dicatat pada kuesioner. Data yang diperoleh, kemudian dihitung keterandalan gedung dan intensitas kerusakan bangunan gedung. Hasil penelitian menunjukan bahwa keterandalan bangunan gedung permukiman di empat perumahan di Jakarta Selatan sebagian besar berkategori sedang. Sementara itu intensitas kerusakannya berkisar antara 34.65%sampai dengan 44.8%.
Keyword: Construction, Demage intensity, Structure, Model houses, Building could, Intensity, Serangan rayap, Kayu lapuk

Judul: Frekuensi dan Intensitas Serangan Rayap Tanah pada Bangunan Gedung milik Pemerintah Provinsi DKI Jakarta.
Abstrak: Jakarta is a province with the most developed infrastructure in Indonesia, including its building constructions. However, termites attack were often found on the buildings in this region. This study was conducted to investigate the frequency and intensity of termites attack on Jakarta Government’s buildings. The targeted buildings in this research were selected by four stage stratified random sampling technique. Termites speciemens were collected from attacked buildings, then identified according to (Ahmad 1958). The results of this research showed that the frequency of termites attack on Jakarta Government’s buildings reaches 16.67% with the degree of infestation could classified as light infestation. Compared to other building components, windows sills has the highest termites attack rate (14.58%), followed by door sills (8.34%), and roof truss (4.16%). In addition, Microtermes inspiratus Kemner (Isoptera: Termitidae) have been found as the most frequent termite species attacking the buildings in Jakarta Province with infestation frequency reaches 77.8%, followed by Coptotermes curvignathus Holmgren (Isoptera: Rhinotermitidae) with infestation frequency of 22.22%. The highest intensity of termites attack occurred in Cililitan Village, Subdistrict Kramat Jati, East Jakarta.
Keyword: buildings, Jakarta, termites

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
Keyword: