Judul: Pembacaan KWH meter otomatis berbasis mikrokontroler menggunakan sensor infra merah
Abstrak: The customer data acquisition system used by the PLN still uses manual methods, which opens the possibilities for an error to happen. These errors is caused by the manual reading oh the KWH meter it self. And it can happen while entering the data to the database, because it were manually inputted.
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Judul: Peracangan Alat Pencatatan Pemakaian Listrik Digital
Abstrak: Penelitian ini dilakukan untuk merancang sebuah alat pencatatan pemakaian listrik (meteran listrik) digital untuk penggunaan skala kecil (rumah tangga) dengan akurasi yang diperoleh dari hasil pengujian adalah 98%. Meteran listrik ini juga memiliki kemampuan untuk berkomunikasi dalam jaringan karena meteran listrik memiliki fitur device addressing yang memungkinkan pembedaan antara meteran listrik yang satu dengan yang lainya. Selain itu, meteran listrik juga dapat berkomunikasi dengan PC (Personal Computer) melalui komunikasi serial dengan interface RS232 melalui aplikasi software yang telah dibuat dalam penelitian ini. Variabel listrik yang dapat dibaca oleh meteran listrik ini adalah akumulasi pemakaian energy, tegangan RMS, arus RMS, daya aktif, daya reaktif, daya total, faktor daya, dan frekuensi.
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Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.