Judul: Manajemen pengendalian penduduk pendatang dalam upaya perbaikan lingkungan kota balikpapan
Abstrak: Balikpapan City is one city that high levels of urbanization. Pull factors are the most prominent of the City of Aberdeen is the city's economy relies on oil and gas industry, commerce and services. The phenomenon of the high number of immigrants, it is necessary to control the management of migrants in order to achieve balance and harmony with the population carrying capacity. The results showed that the City of Aberdeen population not spread evenly throughout the district. With a population that exist today and the next few years is still sufficient capacity of West District Balikpapan, East and North. As for Aberdeen South and Central districts already exceed the carrying capacity. Aberdeen is also able to accommodate the arrival of job-seekers as the capacity of labor is very minimal. Proper management of population control is redirecting immigrants to the county that still has the capacity of the environment and have the availability of water. Since the capacity of labor is very minimal, then you should only accept immigrants Aberdeen City who have migrated for reasons to move the task from city to city Balikpapan. Issue of the settlements, one with a built Rusunawa not slums located on the river, forming a more healthy environment.
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Judul: An analysis of environmental quality of medium and small cities in Kalimantan.
Abstrak: Based on the 2010 census, Kalimantan region inhibited by 13 787 831 people where 5 799 291 people or 42.06 % among them live in urban areas. The population growth occurred because of the rising birth rate, urbanization from rural areas, as well as immigrant that coming from other areas. On one side, this condition contributes positively to abundant availability of human resources. On the other hand, this condition causes problems or negative impacts on the environment such as pollution and more intense use of urban land. Through good urban environment management, these negative impacts can be reduced. This study conducted in order to achieve a sustainable city through good environmental management. This study conducted in order to achieve a sustainable city through good environmental management. Based on population classifications, Kalimantan region consist of 52 cities. These cities divided into 5 big cities, 4 medium cities and 43 small cities. Until now, there was a tendency of urban environmental management research more focused on big cities and very limited similar researches have done in medium and small cities. As the result, information about environment management in medium and small cities is generally difficult to obtain. Therefore, in these researches medium and small cities were chosen to be the object of the research. The objectives of this research are : (1) analyzing and grouping medium and small cities in Kalimantan based on common characteristics of the environment, (2) analyzing factors that affect the medium and small cities environmental quality index in Kalimantan, (3) analyzing relationship between environmental budget allocations, solid waste management budget allocation and city environmental quality index, (4) analyzing relationship between population density and city environmental quality index and (5) developing direction in improving environmental quality for medium and small cities. The research was conducted over 14 months in the period of June 2012 to July 2013. Research area covers 47 cities in Kalimantan, which consist of 4 medium cities and 43 small cities. Analysis of the data used in this study including: (1) Cluster analysis, (2) Principal component analysis and (3) Panel data analysis. Cluster analysis was used to group cities based on common characteristics related to environmental management. The analysis obtained 6 or 12.77 % of the cities belongs to best category, 7 or 14.89 % of the cities belongs to good category, 19 or 40.43 % of the cities belongs to sufficient category, 11 or 23.40 % of the cities belongs to bad category while the rest 4 or 8.51 % are belongs to the worst category. Principal component analysis showed that management of public area such as city park and traditional market has greatest impact on city environment quality index. While management of private area such as citizen settlements has less positive impact on city environment quality index. Panel analysis was used to get correlation between environmental management budget allocation, solid waste management budget allocation, urban area population density and environmental quality index of the cities. As the result environmental management budget allocation and solid waste management budget allocation have positive correlation with city environmental quality index.
Keyword: budget allocations, green open space, population density, solid waste, urban environment quality

Judul: Characterization and Expression of Tomato infectious chlorosis virus Coat Protein Gene in Escherichia coli
Abstrak: Tomato infectious chlorosis virus (TICV) is a member of Crinivirus genus. TICV infects tomato crop in Garut and Cipanas, West Java Indonesia. Tomato initially showed bright interveinal yellowing symptoms. The coat protein gene (CP-TICV) was amplified by RT-PCR from total RNA extracted from infected tomato leaves and the amplified fragment was cloned and completely sequenced. The fragment was subsequently subcloned into the pET-21b expression vector. The recombinant plasmid was transformed to Escherichia coli strain BL21(DE3)pLysS to express the coat protein. The coat protein fused to a 6xhistag, was purified by affinity chromatography using a NiNTA spin column. The identity of the purified protein was confirmed by SDS-PAGE. In this experiment, 792 bp of CP-TICV gene of TICV virus has been successfully cloned, sequenced, and expressed in E. coli. Based on nucleotide sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain strain and 99% identity to North America, France, and California strains and based on amino acid sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain, North America, France strains and 99.2% identity to California strain. CP-TICV showed over expressed in E. coli when it is induced with 1 mM IPTG and incubated at 37oC. Purified CP-TICV-Histag recombinants protein sized 29 kDa based on SDSPAGE analysis.
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