Judul: Pengelolaan Lanskap Taman Margasatwa Ragunan Jakarta Selatan
Abstrak: Taman Margasatwa Ragunan (TMR) has been serviced as a conservation, education, research, and outdoor recreation functions. Meantime an evaluation related to the landscape management in TMR is needed to know either management is good and compatible or not. Therefore, the study to identify the aspects that affect the landscape management in TMR has been done by having internship activity. The data needed in this internship include the physical/biophysical aspects, management aspects, legal aspects, economic and social aspects of the TMR. Thus, it will produce a landscape management strategy for TMR. Analysis of data is performed by using the descriptive analysis, visitor comfort analysis, and SWOT analysis. The results of SWOT analysis found that there are nine point of strategies for landscape management of TMR. TMR has some shortcomings in its management. TMR needs more professional management with support from various parties. TMR is under the responsibility of the Dinas Kelautan dan Pertanian of DKI Jakarta, so that TMR needs more attention from the Government of DKI Jakarta
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Judul: Landscape Planning of Taman Margasatwa Ragunan Jakarta Based on Bioregion
Abstrak: Taman Margasatwa Ragunan (TMR) mempunyai fungsi sebagai tempat konservasi, edukasi, penelitian, dan rekreasi alam. Sejalan dengan upaya menyejahterakan satwa serta memberikan pengalaman di dunia satwa bagi pengunjung, TMR perlu menata kawasan konservasi satwa ex-situ dengan tema lanskap alami yang menyerupai habitat aslinya. Simulasi habitat yang alami dapat terbentuk dari penataan kawasan berbasis bioregion. Oleh karena itu, diperlukan suatu studi untuk mengetahui karakteristik habitat satwa yang mempengaruhi perencanaan lanskap tersebut. Tujuan dari penelitian ini adalah menyusun rencana lanskap TMR berbasis bioregion. Metode perencanaan lanskap dilakukan dengan survei, analisis deskriptif, dan analisis spasial. Tahap analisis yang dilakukan yaitu menganalisis konsep bioregion melalui analisis karakteristik bioklimat yang merupakan faktor signifikan penyusun bioregion, menganalisis karakteristik eksisting kawasan konservasi ex-situ TMR, dan menyusun rencana lanskap. Hasil penelitian berupa perencanaan lanskap kebun binatang yang berupaya menyediakan ruang untuk mendukung fungsi utama dari kebun binatang tersebut. Perencanaan lanskap TMR ini menghasilkan rencana ruang, rencana aktivitas dan fasilitas, rencana sirkulasi, dan rencana vegetasi., Taman Margasatwa Ragunan has been serviced as a conservation, education, research, and outdoor recreation functions. TMR arranges an ex-situ wildlife conservation area with a natural landscape resembles its natural habitat. This is in line to supports the welfare of its animal, and provide to visitors a total environtmental experience and an opportunity to rediscover the beauty and value of natural fauna and flora in urban oasis. In line with the efforts, TMR needs to organize ex-situ animal conservation areas with natural landscape themes that resemble their natural habitat. Natural habitat simulation can be formed from the arrangement of bioregion-based areas. Therefore, a study is needed to determine the characteristics of animal habitats that affect landscape planning. The purpose of this study is to develop a bioregion-based TMR landscape plan. This landscape planning method is based on a field survey, descriptive and spatial analysis. The results of research in the form of zoo landscape planning which seeks to provide space to support the main functions of the zoo. The TMR landscape planning then produces spatial plans, activity and facility plans, circulation path plans, and vegetation plans.
Keyword: bioregion, ex-situ conservation, habitat simulation, landscape planning, Taman Margasatwa Ragunan

Judul: Influenza Specific Immunoglobulin Y Purification from Egg Yolk with Sodium Chloride Precipitation Method
Abstrak: Imunoglobulin Yolk (IgY) yang terdapat dalam kuning telur telah banyak dimanfaatkan untuk imunoterapi dan imunodiagnostik. Salah satu kendala dalam pemurnian IgY dari kuning telur adalah pemisahan Ig dengan lemak, sehingga teknik pemurnian Ig selalu berkembang. Penelitian ini bertujuan untuk melakukan pemurnian IgY dari kuning telur dengan metode pengendapan menggunakan sodium chloride (NaCl) serta melakukan karakterisasi IgY spesifik influenza. Sodium chloride dipilih karena bahan kimia ini relatif murah, ramah lingkungan, dan tidak merusak imunoaktivitas IgY. Kuning telur yang digunakan dalam penelitian ini berasal dari telur ayam layer yang divaksinasi dengan vaksin influenza Flubio® yang berisi virus Influenza A/H3N2, A/H1N1 dan B/Washington. Pemurnian IgY hanya dilakukan pada kuning telur yang mengandung antibodi spesifik influenza. Deteksi keberadaan antibodi spesifik influenza ditentukan dengan uji agar gel presipitasi (AGP) dan metode enzyme linked immunosorbent assay (ELISA). Konsentrasi IgY hasil pemurnian dihitung dengan spektrofotometer dan karakterisasi protein dilakukan dengan metode Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Konsentrasi protein dari hasil pemurnian IgY memiliki nilai rata – rata 50,403 mg/mL. Hasil SDS-PAGE menunjukkan enam pita protein, yaitu struktur IgY utuh (174,63 kDa), Apoprotein-LDL (129,77 kDa), rantai berat IgY (66,54 kDa), Fab ( 47,65 kDa), rantai ringan IgY (21,85 kDa), dan β-livetin (36,74 kDa). IgY hasil pemurnian masih dapat berikatan dengan antigen influenza berdasarkan uji ELISA. Hasil penelitian ini menunjukkan bahwa pemurnian IgY menggunakan metode pengendapan NaCl dapat memurnikan IgY dengan struktur utuh, meskipun masih ada dua protein tidak spesifik hasil pemurnian. IgY yang dapat berikatan dengan antigen influenza mengindikasikan prospek pemanfaatannya untuk pencegahan influenza dan imunodiagnostik., Yolk immunoglobulin (IgY) contained in egg yolk has been widely used for immunotherapy and immunodiagnostics. One of the obstacles in IgY purification from egg yolk is separating Ig from fat, so that IgY purification techniques are always developing. This study aimed to purify IgY from egg yolk using sodium chloride (NaCl) precipitation method and to characterize influenza-specific IgY. Sodium chloride was chosen because this chemical is relatively unexpensive, environmentally friendly, and does not impair IgY immunoactivity. The egg yolks used in this study were collected from layer eggs vaccinated with the Flubio® influenza vaccine, which contains the Influenza A/H3N2, A/H1N1 and B/Washington viruses. IgY purification was only carried out on egg yolks containing influenza specific antibodies. The presence of influenza-specific antibodies was determined by the agar gel precipitation (AGP) test and the enzyme linked immunosorbent assay (ELISA) method. The purified IgY concentration was determined using spectrophotometer, and protein characterization was performed using the Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) method. Protein concentration from purification has a mean value of 50,403 mg/mL . SDS-PAGE result showed six protein bands, namely intact IgY structure (174,63 kDa), Apoprotein-LDL (129,77 kDa), IgY heavy chain (66,54 kDa), Fab (47,65 kDa), IgY light chain (21,85 kDa), and β-livetin (36,74 kDa). The purified IgY still able to bind with influenza antigen based on the ELISA test. The results of this study indicate that purification of IgY using the NaCl precipitation method can purify IgY with intact structure, although there are still two unspecific proteins in the purification results. The resulting IgY can bind to influenza antigens indicating the
Keyword: Immunoglobulin Y, influenza, egg yolk, purification, sodium chloride