Judul: Keragaan Sifat Kimia Tanah di Bawah Vegetasi Mangrove dan Hubungannya dengan Air Tanah Serta Perkembangan Akar dari Beberapa Zona Hutan MAngrove Banyuwedang Kabupaten Buleleng, Bali
Abstrak: Penelitian bertujuan untuk mempelajari dan mengetahui perbedaan sifat kimia tanah di bawah vegetasi mangrove dan hubungannya dengan sifat kimia air tanah dan perkembangan akarnya. Penelitian telah dilakukan pada beberapa zona mangrove yaitu Rhizophora, Ceriops, Avicennia dan Lumnitzera di hutan mangrove Banyuwedang, Kabupaten Buleleng, Bali. Pada masing-masing zona diambil contoh tanah pada lapisan atas (0-30 cm) dan lapisan bawah (30-60 cm) yang dilakukan secara komposit, dan kemudian diambil contoh air tanah serta akar mangrovenya. Untuk melihat keeratan hubungan beberapa sifat kimia tanah, air tanah dan akar mangrove dilakukan melalui Analisis Regresi Berganda.
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Judul: Pengaruh Sifat-sifat Tanah terhadap Zonasi Hutan Mangrove Banyuweclang, Kabupaten Buleleng, Bali
Abstrak: Peneli tian ini bertu,juan mempelaj ari sifat-sifat tanah clan sifat-sifat vegetasinya. Selanjutnya clilakukan pengujian statistik untuk menetapkan sifat-sifat tanah mana yang berbecla nyata antara keempat lahan mangrove clan sifatsifat tanah yang menyebabkan terbentuknya zonasi hutan mangrove tersebut. Disamping itu dipelajari pula sifatsifat tanah yang berpengaruh terhadap kanclungan unsur dalam daun mangrove dan perkembangan akarnya. Sifat-sifat tanah lapisan atas ,,ang menjadi pembecla antara lahan Rhizophora dengan lahan Ceriops, lahan Avicennia clan lahan Lumnitzera adalah: pH H20, pH KCl, Corganik, N-total, t1g-dd, Na-dd, total basa, KTK, Al-dd, Hdd, Fe, S, FeS2, S03 dan persen debu. Pada lapisan bawah pembeda tersebut masih ditambah dengan P-tersedia, K-clcl dan persen pasir. Pembecla lahan Rhizophora dengan lahan Ceriops masih ditambah dengan P-tersedia pacla tanah lapisan atas. Pembecla lahan Rhizophora clengan Avicennia clan lahan Lumnitzera ditambah clengan persen pasir pacla tanah lapisan atas clan Ca-dd pada tanah lapisan bawah. Sifat tanah yang mempengaruhi terbentuknya zona Rhizophora adalah pH-CaC12 dan persen liat pada tanah lapisan atas dan H-dd pada tanah lapisan bawah_ Sifat-sifat tanah yang menjadi pembed.a antara lahan Ceriops dengan lahan Avicennia adalah: C-org.smik, N-total, K-dd dan KTK pada tanah lapisan atas serta C-organik, Ntotal, K-dd, KTK dan persen pasir pada tanah lapisan bawah_ Pembeda antar·a lahan Ceriops dengan lahan Lumnitzera adalah: C-organik, N-total, Na-dd dan persen liat pada tanah lapisan atas serta C-organik, N-total, Mg-dd, Na.-dd, persen debu, persen liat dan total basa pada tanah lapisan ba.wah_ Terbentuknya · zona Ceriops dipeng.aruhi oleh: basa-basa pada tanah lapisan atas serta C-organik dan per-sen debu pada tanah lapisan b.awah _ Sifat-sifat tanah yang menjadi pembeda antara lahan Avicennia dengan lahan Lumnitzera adalah: Mg-dd, K-dd, Na-dd, total basa dan KTK pada tanah lapisan atas serta Mg-dd, K-dd, Na-dd, H-dd dan total basa pada tanah lapisan bawah. dst ...
Keyword: Zonasi Hutan Mangrove, Banyuweclang

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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