Judul: Genetic Polymorphism of Growth Hormone (GH) in Association with Carcass Quality of Aceh Cattle
Abstrak: This study was conducted in order to identify polymorphism of growth hormone gene and to characterize nucleotide changes and its position in the DNA sequence of the exon five in the bovine growth hormone gene. In addition, this study was done to determine the association of GH/AluI polymorphism with carcass quality and to describe D-Loop of mtDNA and microsatellite alleles in Aceh cattle. A total of 242 DNA genome samples were extracted from four Aceh cattle population, i.e., Banda Aceh (29), Aceh Besar (30), Indrapuri (129), and Saree (54), while for sample comparison was derived from the Gen Bank. The bGH gene was sequenced to identify new single nucleotide polymorphism (SNP). The sequence data were analyzed using BLAST and MEGA 4 software, and the PCR-RFLP was used to amplify 404 bp of GH gene. D-Loop sequences of mtDNA amplification were done by using specific primer with the PCR product of 980 bp, and the dnaSP program was used to build the NJ tree and to identify the haplotype. Three markers of BM1824, SPS115, and ILSTS028 were used for microsatellite DNA genotyping. The data of genotyping was analyzed using GeneMapper versi 4.0 software and Excel program. The result showed that one new SNP was found in the exon five of bGH gene, which located at position of 2.230 bp (C/T). Genotype frequencies of SNP in position 2.230 bp of Aceh cattle were 0.36; 0.14; and 0.50; for CC, TT, and CT respectively. On the contrary, the LL genotype was the only genotype which found in other Aceh cattle population. This finding indicated that there was not evidence of polymorphism of GH/AluI in Aceh cattle, and there was not correlation of GH/AluI gene with carcass quality of Aceh cattle. It could be affected by small number of sampling size. D-Loop mtDNA analyses showed that there was 27 haplotypes, and 28 Aceh cattle integrated into group of Buthan, China, India, and Zebu cattle with maternal origin of Bos indicus and one sample was from Bos taurus. These finding could be assumed that Aceh cattle has more genetic introgression of Bos indicus compared to that Bos taurus breeds. Microsatellite analyses showed that the average number of allele per locus was 7.6. The genotype of Indrapuri population was higher than those of Banda Aceh and Saree population. Based on the microsatellite alleles analyses, the frequency of C alele locus of SPS115 and ILSTS028 was higher in Indrapuri population. Nevertheless, these locus could be uses for further study to get more information about polymorphism in Indrapuri population. However, this study suggests that GH gene could be possible used as genetic marker.
Keyword: Growth hormone, Carcass quality, Bos sondaicus, Bos javanicus

Judul: Identifikasi keragaman Gen Growth Hormone Receptor (GHR AluI) pada Sapi Friesian Holstein di BIB Lembang, BBIB Singosari, dan BET Cipelang.
Abstrak: Growth hormone receptor (GHR) is one factor affecting animal growth. GHR is required by growth hormone (GH) to carry out its effect on target tissues. The objective of this study was to identify polymorphism of the Growth Hormone Receptor gene in Holstein Friesian (HF) dairy cattle and beef cattle. Genotyping was performed on 126 animals. These consisted of HF 89 heads from BIB Lembang (17), BBIB Singosari (32), and BET Cipelang (40); as well as for 37 heads of four breeds of beef cattle including for Simmental (13), Limousin (14), Brahman (5), and Angus (5) for a comparison. Varian genetic of the GHR gene was identified by PCR-RFLP method using AluI restriction enzyme cutting the base position at AG|CT. Single nucleotide polymorphism (SNP) had been found in exon 10, coding for the cytoplasmic domain of GHR, which was located at position 81 bp (A/G) induced amino acid substitutions Ser/Gly. The product of amplification of GHR was 298 bp. Genotyping the GHR gene produced two alleles (A and G) resulting three genotypes (AA, AG and GG). The genotype AA was dominant in HF cattle from all locations, and also in Brahman and Limousin cattle from BET Cipelang. All of HF dairy cattle were in Hardy-Weinberg disequilibrium (χhit 2>χ0,05 2), in contrast, Simmental and Limousin were in Hardy-Weinberg equilibrium. The Hardy-Weinberg in Brahman and Angus values could not be calculated. Heterozigosity values in HF and beef cattle were low (ho<he). In beef cattle, Angus had highest heterozigosity value (0,600), while Brahman had the lowest heterozigosity value (0.000). The conclusion was that the variability of GHR|AluI gene can be used as a basic information for selection in growth caracteristic in the HF and beef cattle. Keywords: Beef cattle, Genetic polymorphism, GHR|AluI gene,
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Judul: Nanokristalin Pirit (FeS2) sebagai Material Semikonduktor Fotovoltaik.
Abstrak: Teknologi fotovoltaik adalah suatu proses yang dapat mengubah sinar matahari menjadi energi listrik dengan media semikonduktor. Penggunaan sel surya untuk fotovoltaik hanya dapat mengonversi radiasi matahari menjadi energi listrik kurang dari 20%. Efisiensi akan meningkat ketika material semikonduktor untuk fotovoltaik dibuat menjadi ukuran nano. Pirit merupakan salah satu material semikonduktor fotovoltaik yang memiliki energi pita celah (Eg) dalam kisaran 0.8–0.95 eV. Energi pita celah tersebut terbukti meningkat ketika disintesis dengan metode hidrotermal menggunakan beragam molar [S]:[Fe] pada suhu 200 °C dalam 24 jam. Nilai energi pita celah yang dihasilkan adalah 1.58–2.073 eV. Ukuran nanokristalin pirit yang dihasilkan berada pada kisaran 21.04–58.77 nm menurut data Particle Size Analyzer. Besarnya ukuran nanokristal pirit yang tumbuh kurang dari 50 nm merupakan poin penting untuk aplikasi fotovoltaik.
Keyword: Hidrotermal, fotovoltaik, nanokristal, pirit