Judul: Persistensi vaksin DNA penyandi glikoprotein 25 yang diberikan melalui pakan buatan pada ikan mas (Cyprinus carpio)
Abstrak: Koi Herpes Virus (KHV) is a major viral pathogen that infects common carp and koi. KHV disease outbreak in almost all centre of common carp culture in Indonesia and caused mass mortality. DNA vaccination method is one of ways to cope with KHV infection. Vaccines were commonly given by injection. In this research, persistence test of DNA vaccine (pMBa-GP25) were carried out by oral feeding on common carp. DNA vaccine persistence test were done using PCR method with the specific primer for GP25 gene. Escherichia coli DH5α bacteria containing pMBa-GP25 DNA vaccine were cultured in four treatment mediums namely 2xYT, LB polypeptone, LB triptone and TSB. Bacteria densities were counted with spectrophotometer using 580 nm wave lengths. The results showed that an efficient culture media for E. coli was LB tripton. Furthermore, GP25 DNA was detected in kidney and spleen of vaccinated fish. Level of DNA vaccine that could be detected was 7,56 ng (amount of DNA copy 1,598 x 1010 copies).
Keyword: 

Judul: Efikasi Vaksin DNA Penyandi Glikoprotein Koi Herpesvirus pada Ikan Mas Stadia Benih melalui Perendaman
Abstrak: Koi herpesvirus (KHV) is a herpesvirus which especially infects and causes mass mortality to koi and common carp. Therefore the protection of carp from KHV infection is urgently needed. In this study, we developed an application of DNA vaccine containing gene encoding KHV glycoprotein by immersion method to increase survival of common carp against KHV infection. As many as 400 of 30-day-old common carp juveniles were immersed in the water containing 1.3 x 108 CFU/ml of the killed Escherichia coli cells carrying DNA vaccine. Treatments performed were variation of duration and frequency of DNA vaccine immersion; as follows: 1x 30 minutes, 1x 60 minutes, 1x 90 minutes, 2x 90 minutes, and 3x 90 minutes of immersion treatments. Reverse transcription polymerase chain reaction (RT-PCR) analysis showed that DNA vaccine was successfully expressed among the vaccinated fish. Twenty eight days post vaccination, the fish were challenged by injecting 10-4 ml/fish of KHV. The result demonstrated that vaccination by 30 minutes immersion; allowed the 61% of fish survived, this was significantly different (P<0.05) compared to control (without vaccination), but insignificantly different (P>0.05) compared to those of other treatments. The relative percent survival of vaccinated fish were also similar among different treatments (P>0.05). Vaccination by immersion have increased the survival rate about 2 fold compared to unvaccinated fish control (26.67%). Thus, DNA vaccine was effective delivered by immersion for 1x 30 minutes, and this technique can be useful to level up the resistance to KHV infection in common carp juveniles.
Keyword: 

Judul: Pemanfaatan Buluh Bambu Tali Sebagai Komponen Pada Konstruksi Rangka Batang Ruang
Abstrak: Salah satu bentuk konstruksi yang banyak digunakan sebagai konstruksi rangka atap adalah konstruksi rangka batang (truss) yang kemudian berkembang menjadi konstruksi rangka batang ruang (space truss). Dengan kelurusan bambu yang terbatas, buluh bambu sesuai untuk digunakan pada konstruksi rangka batang ruang, karena konstruksi ini tersusun dari komponen-komponen yang relatif pendek. Pada konstruksi ini, komponen-komponen batang dihubungkan secara sendi, sehingga beban yang bekerja pada batang hanya gaya aksial tekan dan tarik. Bambu diketahui mempunyai kuat tarik yang tinggi dengan kuat geser serta kuat belah yang rendah, sehingga dari sudut mekanika bahan cocok untuk dimanfaatkan pada konstruksi rangka batang. Bentuk bambu yang berupa tabung agak tirus (tappered) dengan diameter yang beragam, selama ini dianggap sebagai hambatan dalam pemanfaatannya di bidang konstruksi. Selain itu adanya buku dengan jarak yang tidak seragam menjadi kendala dalam membuat sambungan, khususnya sambungan yang dapat dianalisa secara mekanika.
Keyword: buckling properties, tensile strength, resistance force, inner part of bamboo, West Java