Judul: Status Anemia dan Prestasi Belajar Siswi SMU I Kuala Kapuas Kabupaten Kapuas
Abstrak: Penelitan ini secara umum bertujuan untuk mengetahui hubungan status anemia dengan prestasi belajar siswi SMUN 1 Kuala Kapuas. Adapun tu.juan khususnya adalah mengidentifikasi status anemia siswi, mengkaji status sosial ekonomi keluarga siswi berdasarkan status anemia, mengkaji kebiasaan makan siswi berdasarkan status anemia, mengidentifikasi status kesehatan siswi berdasarkan status anemia, membandingkan prestasi belajar siswi berdasarkan status anemia, dan mengetahui hubungan status anemia dengan prestasi belajar siswi.
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Judul: Status Anemia dan Prestasi Belajar Siswa/i Madrasah Aliah Pesantren Al-Hamidiyah Depok
Abstrak: Penelitian ini bertujuan untuk mempelajari status anemia dan prestasi belajar siswa/i Madrasah Aliah Pesantren Al-Hamidiyah Depok. Penelitian ini menggunakan desain cross sectional dengan melibatkan 63 siswa dan dilaksanakan pada bulan Februari sampai Maret 2016. Contoh adalah siswa dan siswi kelas satu Madrasah Aliah Pesantren Al-Hamidiyah Depok. Pengumpulan data karakteristik contoh, sosial ekonomi keluarga, dan konsumsi pangan dilakukan dengan cara pengisian kuesioner; kadar hemoglobin contoh didapatkan dengan menggunakan alat Easy Touch GcHb; dan prestasi belajar diestimasi berdasarkan nilai rata-rata ujian tengah semester yang diperoleh dari sekolah. Hasil studi menunjukkan bahwa tingkat kecukupan energi dan zat gizi dari kedua kelompok contoh masih dibawah standar AKG 2013 dengan tingkat kecukupan energi (68.4%), protein (78%), kalsium (27.4%), fosfor (44.4%), vitamin C (25.4%), dan zat besi (63%), kecuali vitamin A (128.2%). Sebagian besar contoh (65.1%) mengalami anemia dan lebih dari tiga perempat (73.2%) contoh yang anemia adalah perempuan. Hasil uji korelasi menunjukkan bahwa terdapat hubungan signifikan positif antara tingkat kecukupan zat besi dengan kadar Hb (p<0.05, r=0.522) tetapi tidak terdapat hubungan antara tingkat kecukupan protein dengan kadar Hb (p>0.005, r=0.078). Tidak terdapat perbedaan signifikan (p>0.05) prestasi belajar dan tingkat kecukupan zat besi antara contoh anemia dan contoh yang tidak anemia.
Keyword: prestasi belajar, remaja, status anemia

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable