Judul: Kesesuaian Ruang Terbuka Hijau pada Rencana Tata Ruang Wilayah dan Tutupan Lahan di Kota Bekasi
Abstrak: Changes in land cover in Bekasi City, which grew rapidly resulting in a potential unsuitable between the Spatial Planning (RTRW) with existing land cover. The purpose of this study was (1) To analyze changes in land cover classification results using Landsat 7 ETM + and Landsat 8 OLI in Bekasi City in 2003, 2009 and 2013, (2) To analyze the suitability of green open space (RTH) on theland cover with the Spatial Plan (RTRW) of Bekasi City. The method used was the digital image analysis using supervised classification method, and the thematic change analysis using geographic information system The results showed that the suitability of green open space on Spatial Planning and land cover between 2003, 2009 and 2013 indicated the most appropriate RTH between Spatial Planning with land cover was in 2009 in the amount of 312.54 Ha while the most unsuitable was the RTH of 2003 in the amount of 129.89 ha
Keyword: Bogor Agricultural University (IPB), Bekasi City, green open spaces, cover change

Judul: Suitability of Green Open Spaces on Spatial Patterns Using SPOT-7 Imagery in Sukaraja and Babakan Madang Districts Bogor Regency
Abstrak: Perkembangan daerah urban yang cukup tinggi berpengaruh pada percepatan pembangunan daerah, dan mengakibatkan perubahan tutupan lahan. Perubahan tutupan lahan terkendali dengan adanya rencana tata ruang wilayah (RTRW). Penelitian ini bertujuan menganalisis perubahan tutupan lahan di Kecamatan Sukaraja dan Babakan Madang, serta analisis kesesuaian tutupan ruang terbuka hijau (RTH) dengan pola ruang RTRW Kabupaten Bogor. Analisis perubahan tutupan lahan dilakukan dengan mengklasifikasi secara visual menggunakan citra SPOT-7 tahun 2016 dan 2021. Hasil klasifikasi tutupan lahan memiliki nilai indeks kappa sebesar 0,91. Perubahan luas tutupan lahan menjadi lahan terbangun terus bertambah, sedangkan luas RTH menurun. Luas RTH mengalami penurunan sebesar 5,41% di Kecamatan Sukaraja dan 2,79% di Kecamatan Babakan Madang. Kesesuaian luas RTH dengan pola ruang RTRW di Kecamatan Sukaraja sebesar 93,71% pada tahun 2016 dan mengalami penurunan pada tahun 2021 menjadi 91,83%, sedangkan di Kecamatan Babakan Madang sebesar 97,87% pada tahun 2016 dan mengalami penurunan menjadi 97,14% pada tahun 2021. Luas RTH di Kecamatan Sukaraja dan Babakan Madang masih memenuhi aturan yang ditetapkan pemerintah yaitu minimal 30% dari luas wilayah perkotaan., The relatively high development of urban area has an effect on acceerating regional development, resulting in changes in land cover. Changes of land cover are controlled by having a regional spatial planning (RTRW). This study aims to analyze the suitability of green open space (RTH) cover with the spatial patterns of the Bogor Regency spatial plan. Analyze of land cover change was carried out by visual classification method using SPOT-7 imagery for 2016 and 2021. The results of land cover classification have a kappa index value of 0,91. Changes in land cover to built-up land have continued to increase, while the area of green open space decreased. The area of green open space decreased by 5,41% in Sukaraja District and 2,79% in Babakan Madang District. The suitability of the area green open space with spatial patterns in Sukaraja District was 93,71% in 2016 and decreased in 2021 to 91,83%, while in Babakan Madang District it was 97,87% in 2016 an decreased to 97,14% in 2021. The area of green open space in Sukaraja an Babakan Madang sub-district still meets the goverment’s regulations, namely a minimum of 30% of the urban area.
Keyword: green open space, index kappa, land cover change, regional spatial plan, SPOT-7 imagery

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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