Judul: Komposisi jenis tumbuhan bawah pada tegakan sengon (Paraserianthes falcataria, L. Nielsen) (Studi Kasus di Areal Kampus IPB Darmaga, Bogor)
Abstrak: Sengon (P. falcataria) merupakan jenis pohon yang bernilai ekonomi tinggi dan berpotensi untuk pengembangan agroforestry. Informasi mengenai keanekaragaman jenis tumbuhan bawah pada tegakan sengon belum banyak diketahui. Tumbuhan bawah selain memiliki fungsi ekologi dalam keseimbangan ekosistem hutan juga dapat berperan sebagai gulma dalam budidaya tanaman monokultur. Selain itu beberapa jenis telah diidentifikasi sebagai tumbuhan bermanfaat. Penelitian ini bertujuan untuk mengetahui komposisi dan keanekaragaman jenis tumbuhan bawah pada tegakan sengon.
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Judul: Komposisi Jenis Tumbuhan Bawah pada Tegakan Sengon (Paraserianthes falcataria, L. Nielsen) (Studi Kasus di Areal Kampus IPB Darmaga, Bogor)
Abstrak: Sengon (Paraserianthes falcataria L. Nielsen) is a tree species which has high economy value and potential for agroforestry development. Information on the diversity of undergrowth species at sengon stands has not been much known. Undergrowth vegetation have ecological functions to keep the ecosystem balance and as addition, it can also take a role as a weed in the monoculture plants cultivation. In other hand, some undergrowth species have been identified as beneficial plants. This research aims to know the composition and diversity of undergrowth species at sengon stands. Results of inventory at the age of 5 years sengon stands, found 41 species from 23 families which dominated by the type of Ottochloa nodosa, Paspalum conjugatum, Lepistemon binectarifer, and Asytasia gangetica. At the age of 15 years, found 34 species from 20 families which dominated by the type of Ottochloa nodosa, Oplismenus compositus, Axonophus compressus, and Asytasia gangetica. The diversity levels of species according to the classification of Magurran (1998) at age 5 years sengon stands of 2.11 (medium), while at the age of 15 years sengon stands of 1.86 (low). Meanwhile, in the calculation of community similarity levels, condition of the community based on the number of types and abundance of individuals of each species is different (IS ≤ 75%), with the value 48.42. Among the species found, there are several types of plants which have potency as medicinal plants, such as pacing (Costus speciosus) and nut-grass (Cyperus rotundus), as food resource, such as taro (Colocasia esculenta) and takokak (Solanum jamaicense). The types of cover crops and fodder such as ornamental peanut (Arachis pintoi) and sentro (Centrocema pubescens). The type of ornamental plants such as wedelia (Wedelia trilobata) and taro (caladium bicolor). Furthermore, there is the type of plants which has potency as a fuel source such as iles-iles (Amorphopallus variabilis).
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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