Judul: Potensi Antikanker dan Identifikasi Fraksi Drakorodin Jernang Daemonorops draco
Abstrak: Dragon’s blood Daemonorops sp. has been used as traditional medicines and natural dyes. However, dragon’s bloods of Dracaena sp. and Croton sp. are well-known to have many pharmacological activities in some other countries, including anticancer. This research aimed to determine the toxicity of the extract related to its potential as anticancer and to determine dracorhodin fraction as the main anthocyanin compound in dragon’s blood. The experiment involved extraction, partition, fractionation, and brine shrimp lethality test. The LC50 value of methanol and ethyl acetate extracts were 70 and 570 ppm, respectively. Fractionation of the methanol extract by using column chromatography with gradient elution with n-hexane and ethyl acetate provided 6 fractions. Ultraviolet-visible spectrum of the fraction 6 showed a maximum absorbance at 467 nm, lying in the range of anthocyanin maximum wavelength. Identification by using gas chromatograph-mass spectrometer proved that the presumed dracorhodin existed in this fraction, at 22.45 minutes retention time and with 4.30% abundance.
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Judul: Identification Antioxidant Compounds of Jernang Extract From Rattan Friut Daemonrops acehensis R. using Metabalomic based LC-MS/MS
Abstrak: Jernang merupakan resin yang banyak digunakan dalam pengobatan tradisional karena memiliki beberapa aktivitas biologis, salah satunya sebagai antioksidan. Salah satu spesies penghasil jernang adalah Daemonorops acehensis Rustiami. Tanaman ini banyak ditemukan di Aceh, tetapi belum ada penelitian yang melaporkan senyawa aktif antioksidan ekstrak jernang dari buah D. acehensis menggunakan pendekatan metabolomik. Penelitian ini bertujuan mengidentifikasi senyawa aktif antioksidan ekstrak jernang dari buah D. acehensis menggunakan pendekatan metabolomik berbasis liquid cromatography mass spectrometry/mass spectrometry (LCMS-MS). Jernang dari buah rotan diekstraksi menggunakan metode maserasi dengan pelarut etanol p.a, etanol 70%, etanol 50%, etanol 30%, dan air. Aktivitas antioksidan ditentukan menggunakan metode difenil pikrilhidrazil. Metabolit dalam ekstrak jernang diidentifikasi menggunakan LCMS/MS. Setiap ekstrak didiskriminasi menggunakan analisis data multivariat principal component analysis (PCA), dan senyawa antioksidan diprediksi menggunakan orthogonal partial least squares-discriminant analysis (OPLS-DA). Ekstrak jernang memiliki IC50 yang berkisar antara 52,40±4,35 hingga 662,72±37,86 mg/L. Ekstrak etanol 70% memiliki aktivitas antioksidan paling tinggi, sedangkan air paling rendah aktivitas antioksidannya. Analisis LC-MS/MS berhasil mengidentifikasi secara putatif sebanyak 31 metabolit, yang terdiri atas 25 metabolit golongan flavonoid, 4 metabolit golongan kalkon, dan 2 metabolit golongan fenolik. PCA berhasil mengelompokkan metabolit berdasarkan pelarut pengekstraksinya, sedangkan model OPLS-DA mampu mengklasifikasi metabolit menjadi dua kelompok, yaitu aktif dan tidak aktif antioksidan. Terdapat enam metabolit yang diprediksi berpotensi sebagai antioksidan dari ekstrak jernang, yakni nordrakorodin (1), drakorodin (2), 2,4-dihidroksi-5-metil-6-metoksikalkon (7), (2S)-5-metoksiflavan-7-ol (14), (2S)-5-metoksi-6-metilflavan-7-ol (17), dan 1 senyawa unknown. Keenam metabolit tersebut memiliki nilai p <0,05, log2 fold change >1, dan variable influence on projection >1 yang menujukkan bahwa metabolit tersebut berpengaruh signifikan terhadap aktivitas antioksidannya., Jernang is a dragon blood resin that coats the rattan fruit and is widely used in traditional medicine because it has several biological activities, such as antioxidant. One of the species that produces jernang is Daemonorops acehensis. This plant is commonly found in Aceh, but no studies are reported on the active antioxidant compounds jernang extract from D. acehensis fruit using a metabolomic approach. This study aims to identify the active antioxidant compounds jernang extract from D. acehensis fruit using an liquid cromatography mass spectrometry/mass spectrometry (LC-MS/MS)-based metabolomics approach. Jernang was extracted using the maceration method with p.a ethanol, 70% ethanol, 50% ethanol, 30% ethanol, and water as solvents. Antioxidant activity was determined using the diphenyl picrylhydrazyl method. Metabolites in jernang extract were identified using LC-MS/MS. Each extract was discriminated using principal component analysis (PCA), and antioxidant compounds were predicted using orthogonal partial least squares-discriminant analysis (OPLS-DA). Jernang extract has an IC50 ranging from 52,40±4,35 to 662,72±37,86 mg/L, where 70% ethanol extract has the highest antioxidant activity, while water has the lowest antioxidant activity. LC-MS/MS analysis putatively identified 31 metabolites, consisting of 25 metabolites from the flavonoid group, 4 metabolites from the chalcone group, and 2 metabolites from the phenolic group. PCA succeeded in classifying metabolites based on the extraction solvent, while the OPLS-DA model classified metabolites into two groups, namely active and inactive antioxidants. Six metabolites are predicted to be potential antioxidants from jernang extract, namely nordracorhodin (1), dracorhodin (2), 2,4- dihydroxy-5-methyl-6-methoxychalcone (7), (2S)-5-methoxyflavan-7-ol (14), (2S)-5-methoxy-6-methylflavan-7-ol (17), and 1 unknown compound. The six metabolites had p
Keyword: DPPH, resin jernang D. acehensis, UHPLC Q-Orbitrap HRMS

Judul: Komputerisasi Model Umum Studi Kelayakan Pembangunan Industri
Abstrak: Masa pembangunan pada Pelita IV membawa Indonesia kepada era baru dalam pembangunannya, yang merupakan tahap tinggal landas menuju masyarakat industri.
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