Judul: Pertumbuhan dan Transmisi Gen Pada Ikan Mas Transgenik Hormon Pertumbuhan Generasi Kedua
Abstrak: Transfer gen penyandi growth hormone (GH) merupakan salah satu metode untuk meningkatkan pertumbuhan beberapa spesies ikan budidaya. Ikan mas transgenik gen hormon pertumbuhan ikan nila (tiGH) telah dibuat hingga generasi pertama (F1). Pengembangan selanjutnya untuk produksi ikan mas transgenik homozigot perlu dilakukan. Tujuan penelitian ini adalah untuk mengevaluasi pertumbuhan dan transmisi gen tiGH pada ikan mas transgenik generasi kedua (F2). Ikan F2 dihasilkan melalui persilangan tiga betina F1 transgenik dengan jantan non-transgenik (A), tiga jantan F1 transgenik disilangkan dengan betina non-transgenik (B), dan sebagai kontrol digunakan sepasang induk nontransgenik (C). Ikan dipelihara hingga 60 hari pascatetas. Hasil penelitian menunjukan derajat pembuahan telur, derajat penetasan telur dan kelangsungan hidup ikan tidak berbeda nyata antar persilangan (p>0,05). Laju pertumbuhan panjang dan bobot ikan persilangan A dan B berturut-turut adalah 1,37-1,52 dan 1,31-1,39 kali lebih tinggi dibandingkan kontrol (p<0,05). Jumlah konsumsi pakan dan efisiensi pakan ikan persilangan A dan B berbeda nyata dengan kontrol (p<0,05). Transmisi gen tiGH pada F2 mengikuti pola pewarisan Mendel.
Keyword: Gene transfer, growth hormone, growth, gene transmission

Judul: Introduksi dan Ekspresi Gen Hormon Pertumbuhan pada Ikan Mas Transgenik
Abstrak: Common carp Cyprinus carpio is one of the economically valuable aquaculture fish species in Indonesia. This research was aimed to produce transgenic common carp that grow rapidly by over-expressing the tilapia growth hormone (tiGH) gene. Trangenic common carp were produced by sperm-mediated gene transfer using electroporation method. In this study, two electroporation programs that developed by other researcher were applied to obtain a suitable electroporation method for common carp. The electroporated sperm were then used to fertilize eggs to produce F0 transgenic fish. Transgenic F0 carrying tiGH in their sperm were crossed with non-transgenic fish to produce transgenic first (F1) generation. Identification of transgenic fish was performed by PCR method using specific primer for tiGH gene. Expression of tiGH was analyzed using RT-PCR method and growth measurement at F1 fish generation. The results showed that electroporation of sperm using 50 Volt/cm, and DNA concentration of 25 ng/μl was produced higher percentarge of fish carrying tiGH in their gamet compared to that of another electroporation program (40 Volt/cm, DNA concentration of 50 ng/μl). Five sexually matured males F0 transgenic fish were selected and mated with non-transgenic to produce F1 generation. Percentage of F1 progenies carrying tiGH was 47,2-66,7%. Variated measure of F1 genereations was 10,1->12,0 cm size (22,5%-42,9%), 8,1->10,0 cm size (14,3%-42,9%), 6,1->8,0 cm size (11,9%-29,2%), 4,1->6,0 cm size (0,0%-39,3%). Increasing of growth of F1 was about 1,4-1,87 times for non transgenic fish. Survival of transgenic and non-transgenic F1 was generally similar. RT-PCR analysis showed that the F1 transgenic fish have tiGH gene expression in caudal fin, while no expression in non-transgenic fish were found. Thus, the increased body weight of F1 transgenic common carp was associated with expression of tiGH gene, and this fish can be used as broodstock for the production of fast-grow stable transgenic common carp.
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Judul: Karakteristik curah hujan DKI Jakarta dengan metode Empirical Orthogonal Function (EOF)
Abstrak: Curah hujan di Indonesia dipengaruhi oleh monsun yang digerakan oleh adanya sel tekanan tinggi dan sel tekanan rendah di benua Asia dan Australia secara bergantian. Dalam bulan Desember hingga Februari (DJF) dibelahan bumi utara terjadi musim dingin akibatnya terdapat sel tekanan tinggi di Benua Asia, sedangkan dibelahan bumi selatan pada waktu ini terjadi musim panas, akibatnya sel tekanan rendah di benua Australia. Karena ada perbedaan tekanan udara di kedua benua tersebut maka pada periode DJF bertiup angin dari tekanan tinggi di Asia menuju ke tekanan rendah di Australia, angin ini disebut monsun barat (winter monsoon). Dalam bulan Juni hingga Agustus (JJA) terdapat sel tekanan rendah di Asia dan sel tekanan tinggi di Australia yang menggerakan monsun timur (suntnter monsoon).
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