Judul: Hubungan Peran Community Development Workers dengan Tingkat Keberdayaan Anak Jalanan.
Abstrak: Masalah kemiskinan sudah menjadi salah satu fenomena sosial di seluruh dunia, termasuk di Indonesia. Tingginya angka kemiskinan di Indonesia berdampak pada masalah kesejahteraan sosial, salah satunya yaitu meningkatnya jumlah anak jalanan. Penanganan permasalahan anak jalanan sangat penting untuk dilakukan dan diperhatikan, selain untuk mendapatkan pelayanan kesejahteraan juga untuk menghindari dampak negatif dari tindakan marjinalisasi terhadap anak jalanan.Oleh karena itu, untuk melakukan upaya pemberdayaan anak jalanan diperlukan peran pendamping atau biasa disebut sebagai CD Workers. Tujuan dari penelitian ini adalah untuk menganalisis hubungan peran CD Worker dengan tingkat keberdayaan anak jalanan. Penelitian ini menggunakan pendekatan kuantitatif yang didukung oleh data kualitatif. Data kuantitatif dalam penelitian ini diolah menggunakan uji korelasi Rank Spearman dengan jumlah responden sebanyak 63 anak jalanan yang mendapat pendampingan dari CD Workers melalui program pemberdayaan anak jalanan di Rumah Singgah Sanggar Senja. Hasil penelitian menunjukkan bahwa terdapat hubungan antara peran CD Workers dengan tingkat keberdayaan anak jalanan.
Keyword: CD Workers, empowerment, street children

Judul: Analisis Program Pemberdayaan Anak Jalanan di Rumah Singgah Tabayun, Kecamatan Cibinong, Kabupaten Bogor.
Abstrak: Penelitian ini bertujuan untuk menganalisis hubungan antara karakteristik peserta dengan tingkat efektivitas program pemberdayaan anak jalanan dan menganalisis hubungan tingkat efektivitas program dengan perubahan perilaku anak jalanan. Penelitian ini dilakukan di Cibinong, Kabupaten Bogor dengan menggunakan pendekatan kuantitatif melalui metode survei terhadap 30 responden peserta program di Rumah Singgah Tabayun. Penelitian ini juga didukung dengan data kualitatif dengan metode wawancara mendalam, observasi lapang, dan penelusuran dokumen. Analisis data kuantitatif dilakukan menggunakan uji korelasi Rank Spearman dan Chi-Square. Hasil dari penelitian ini menunjukkan bahwa karakteristik peserta program seperti usia, jenis pekerjaan, dan jam kerja memiliki hubungan dengan tingkat efektivitas program. Hasil uji korelasi menunjukkan bahwa terdapat hubungan antara tingkat efektivitas program dengan perubahan perilaku peserta program di tingkat perubahan pengetahuan. Penelitian ini juga menunjukkan bahwa tingkat efektivitas program di Rumah Singgah Tabayun masih rendah.
Keyword: efektivitas, karakteristik, perubahan perilaku

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable