Judul: Komposisi Jenis dan Struktur Tegakan Hutan Terganggu di Gunung Papandayan, Garut, Jawa Barat
Abstrak: Growth process of forest community to reach balanced condition often undergoes disturbances which create destruction. Papandayan mountain has been known since Dutch colonial government as an area with very rich floristic composition. Research on species composition and stand structure of disturbed forest has never been conducted in this area. The objective of this study is to learn the species composition and stand structure of disturbed forest in Papandayan mountain, whose forests are disturbed both by human and by natural process. Research results show that biodiversity and species richness in sites disturbed by human are higher than those disturbed by nature. Besides that, forest crown stratification pattern in disturbed forest shows that there is no any forest crown which reaches stratum A (>30 meter). They only reach stratum B (20–30 meter) and stratum C (4–20 meter). Crown stratification in disturbed forest never reached stratum A because stratum A constitutes the character of stable forest condition.
Keyword: stand structure, species composition, Papandayan Mountain, disturbed forest

Judul: Identifikasi Gangguan Ekosistem Hutan Gunung Papandayan di Garut, Jawa Barat
Abstrak: Gunung Papandayan is one of the active volcanoes in West Java and it is a conservation area consisting Nature Reserve and Nature Tourism Park. Therefore, the protection of Gunung Papandayan’s forest ecosystem is needed to be done so that the area can be well maintained. This study was conducted on the forest area which was disturbed by the eruption and also by human activities. The level of disturbance was determined by measuring the tree basal area with line plot method. The socio-economic data was collected from 5 villages which borders Gunung Papandayan. The result showed that the presence of disturbance in Gunung Papandayan. The disturbance was occurred by three factors, consisting natural such as eruption, human factor such as forest fires, illegal logging, and forest encroachment, and also accessibility. The level of forest disturbance on the area of eruption, forest fires and forest encroachment was categorized as high level; while at the illegal logging area was categorized as medium level.
Keyword: cause of disturbance, disturbance level, forest disturbance, Gunung Papandayan

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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