Judul: Evaluasi Kemurnian Genetik dengan Marka Mikrosatelit dan Aplikasi Rizobakteri untuk Meningkatkan Produksi dan Mutu Fisiologis Benih Jagung Hibrida
Abstrak: One effort to improve maize production is through the use of high quality maize seed. In order to assure high quality seed, it’s genetic quality must be tested. SSR markers often used in genetic studies of maize plants. Therefore SSR can be used to analyze the genetic purity of maize hybrid seed. Another effort to increase productivity and physiological quality of maize hybrid seeds is using rhizobacteria and phosphate fertilizer. The objectives of this research were 1) to determine SSR markers specific for male and female parents, 2) to know the effectiveness of SSR markers for the genetic purity testing of maize hybrid seeds, 3) to find non phytopathogenic rhizobacteria isolates capable of dissolving phosphate, producing IAA, and improving the physiological quality of seed and seedling growth of female parent of maize hybrid, 4) to assess the effect of rhizobacteria and phosphate fertilizers to increase plant growth and yield female parent of maize hybrid, 5) to investigate the effect of rhizobacteria and P fertilizer for increasing productivity and physiological quality of maize hybrid seed. This research consisted of four stages: (1) Selection of SSR primers specific for female and male parents, and testing of genetic purity of hybrid maize seed using SSR markers, (2) Characterization of rhizobacteria in producing IAA, dissolving unsoluble forms of phosphate, non phytopathogenic (hypersensitive reaction test), and improving the physiological quality of maize hybrid seed, (3) Testing the effectiveness of rhizobacteria and phosphate fertilizer on plant growth of female parent in polybag, and (4) Evaluation the effectiveness of rhizobacteria and phosphate fertilizer to increase productivity and the physiological quality of maize hybrid seed in the field. All stages of the research were conducted from April 2011 until December 2012. Results of experiment 1 showed that: 1) Out of five markers tested, three markers namely phi96100, phi328175 and phi072 produced polymorphic bands and were able to distinguish parental lines of two maize hybrid varieties. SSR marker phi96100 was specific for detection the genetic purity of cv.Bima-4 and phi072 for cv.Bima-3. While phi328175 was specific markers to both maize hybrids. The genetic purity of cv.Bima-3 and cv. Bima-4 were 97.5% and 80% respectively. Results of experiment 2 showed that from 34 rhizobacteria isolates, consisted of Actinomycetes, Bacillus spp., and Fluorescent pseudomonads, five isolates of each group having characteristic of dissolving phosphate, producing IAA, and non phytopathogenic. B28 and B46 isolates increased growth rate and vigour index of female parent seeds of hybrid maize. AB2, ATS4, B28, P14, and P31 isolates were selected based on the ability to improve the physiological seed quality of female parent and the rhizobacteria B42 based on the highest production of IAA. These isolates were then used in the field experiment. Results of experiment 3 showed that phosphate fertilizer did not effect the plant growth, weight of ear, and weight kernel per ear but rhizobacteria B42 isolate was potential to increase plant growth compared the control and other rhizobacteria. B28 isolate was potential to increase weight of ear, and weight kernel per ear. Results of the field experiment showed that at the first planting season (rainy
Keyword: Actinomycetes, Fluorescent pseudomonads, parental lines of maize hybrid, seed storage, SSR marker

Judul: Pengaruh perlakuan rizobakteri pada benih dan tanaman serta pemupukan fosfat terhadap pertumbuhan tanaman tetua betina jagung hibrida
Abstrak: The use of quality seeds from improved varieties will produce plants more productive and efficient. Seed quality constitute of genetic, physical, physiological, and health quality. Phosphate fertilizer can enhance plant growth, however only 10-30% of fertilizer P that can be absorbed by plants. The use of rhizobacteria can enhance phosphate uptake and plant growth. The objectives of this study were to improve the quality of physiological seeds and plant growth hybrid corn using rhizobacteria and phosphate fertilizers. The research has conducted in three stages of experiments in the laboratory, greenhouse and in the field. Experiment used parent of hybrid corn seed cv. Bima-3, namely: Nei9008 (female parent) and Mr14 (male parent). The groups of isolates Actinomycetes, Bacillus spp. and fluorescent pseudomonads are used in these experiments. The experiment steps were: (1) characterization and screening of rhizobakteria, (2) Evaluation of selected rhizobacteria on seed quality and seedling growth of female parent and (3) Effect of rhizobakteria treatment and phosphate fertilizer on plant growth of female parent. Experiment 3 were conducted in two seasons, season I using six isolates selected from Experiment 1 and 2, while the season II using two isolates which indicated the best result from season I. In the field experiment, the seeds has planted in polybags with sterilized soil. Experiment 3 was arranged in Split Plot Design. The main plot consisted of five doses of phosphate fertilizer, which is 0, 75, 125, 175, and 200 kg SP-36/ha (100% of recommended dosage). Subplot is rhizobacteria treatment. The results showed, there were five isolate choosed from 10 isolates of each genus of rhizobacteria based on the criteria of high producing phosphatase, negative hypersensitive reaction test, producing IAA and chitinase. Experiment 2 showed the rhizobacteria B28 and B46 increase growth rate and seed vigor index. The results of Experiment 1 and 2, rhizobacteria AB2, ATS4, B28, P14 and P31 was selected based on the ability to improve the physiological seed quality of female farent and rhizobacteria B42 based on the highest production of IAA (14.4 μg/ml). Field experiment at the season I, there were rhizobacteria ATS4 and B28 increased plant growht compare to other rhizobacteria. The results from season II, ATS4 has potential to promote plant growth with average number of leaf and plant height at 4 weeks after planting 5.2 and 56.88 cm compared to control 4.8 and 55.98 cm respectively. Phosphate fertilizer strongly influenced on the average number of leaves and plant height. In the season II at 2 and 4 weeks after planting, fertilizing with the recommended dose (200 kg SP-36/ha) showed highest average the number of leaves and the plant height compared with other treatments.
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Judul: Kajian Persyaratan Penempatan Jabatan Struktural Eselon Iv dan Iii Di Dinas Kebakaran Dki Jakarta
Abstrak: Dinas Kebakaran Daerah Khusus Ibukota (DKI) Jakarta adalah perangkat pelaksana Pemerintah DKI Jakarta dalam bidang pelaksanaan usaha pencegahan dan penanggulangan kebakaran serta perlindungan keselamatan jiwa termasuk harta benda dari bahaya kebakaran dan bencana lainnya di wilayah Ibukota Jakarta. Dalam melaksanakan tugas pokok dan fungsinya, Dinas Kebakaran DKI memiliki kewenangan dan tanggung jawab, sumberdaya, dana, sarana, prasarana dan personil yang memerlukan keahlian dan keterampilan, peralatan dan perlengkapan yang diatur dalam Peraturan Daerah (Perda) Nomor 9 Tahun 1980 Juncto Nomor 11 tahun 1986. Dimensi dan kompleksitas permasalahan kebakaran, dalam hal ini adalah kebakaran. kota merupakan salah satu bencana bagi di wilayah Kota DKI Jakarta. Terjadinya kebakaran kota semakin lama semakin bertambah seiring dengan perkembangan Jakarta sebagai kota metropolitan. Tingginya insiden-insiden yang terjadi pada beberapa periode belakangan ini, baik variasi jenis maupun penyebab dan akibat kerugian yang ditimbulkannya, memperlihatkan kecenderungan yang juga semakin meningkat yang secara keseluruhan masih merupakan kerugian yang langsung dapat dihitung, belum termasuk kerugian tak langsung sebagai akibat terjadinya insiden-insiden tersebut. Oleh karena itu, Dinas Kebakaran DKI Jakarta selaku organisasi yang berkompeten dalam penanggulangannya harus mengambil langkah-langkah antisipatif yang diperlukan agar pelaksanaan tugas yang berada dalam kondisi yang baik dapat dipertahankan atau bahkan ditingkatkan. Lajunya perkembangan ilmu pengetahuan dan teknologi (IPTЕК), menghadapkan Dinas Kebakaran DKI Jakarta kepada berbagai permasalahan, terutama masalah rendahnya kualitas pelayanan kepada masyarakat. Masalah ini dirasakan oleh masyarakat karena adanya kebijakan zero growth sampai tahun 2000 oleh Menteri Pembinaan Aparatur Negara, sehingga membawa konsekuensi logis bagi unit instansi masing-masing untuk meningkatkan produktivitas kerja dengan memberdayakan sumberdaya manusia yang ada. Usaha untuk meningkatkan produktivitas kerja harus disertai dengan pendidikan dan pelatihan atau dengan perpaduan pandangan antara karyawan dengan pimpinan untuk menciptakan kerjasama yang kondusif, pembinaan efektivitas kerja ke arah pengaturan dan pengusahaan secara maksimal dengan penempatan karyawan atau pegawai atas prinsip penempatan orang yang tepat pada tempat yang tepat (tke right man on the ringt job). Dalam upaya tersebut di atas, Dinas Kebakaran DKI Jakarta telah membuat standar jabatan dengan mengacu kepada National Fire Protection Association (NFPA). Namun demikian, standar jabatan tersebut berbenturan dengan peraturan-peraturan yang berlaku di Pememerintah DKI Jakarta. Benturan tersebut diantaranya adalah untuk karier dalam jabatan struktural Eselon IV dan III harus mengikuti dan lulus dari Diklat ADUM (Administrasi Umum) dan SPAMA (Staf dan Pimpinan Administrasi Tingkat Pertama). Hal ini tentunya membawa dampak tersendiri bagi Dinas Kebakaran DKI Jakarta dan mempengaruhi pelaksanaan penanggulangan kebakaran. Sebagaimana yang tercantum dalam Perda Nomor 11 Tahun 1986 mengenai struktur organisasi Dinas Kebakaran DKI Jakarta, sebagai 'ujung tombak Dinas Kebakaran adalah Kepala Kepala Suku Dinas Kebakaran di lima Wilayah Kotamadya. Untuk meningkatkan kemampuan manajerial dan teknisnya, pejabat-pejabat tersebut hendaknya dibekali kemampuan manajerial dan kemampuan tehnik sebagai fire fighter (petugas pemadam kebakaran) dan penguasaan wilayah kota untuk pengawasan penanggulangan kebakaran. Keberhasilan Dinas Kebakaran dalam melaksanakan tugas pokok dan fungsi akan tercapai apabila didukung oleh peran sumberdaya manusia yang dimiliki. Oleh karena itu, mengingat pentingnya peran sumberdaya manusia dalam tugas operasional Dinas Kebakaran, maka perlu diupayakan adanya suatu rancang bangun sistem pengembangan karier melalui kajian persyaratan penempatan jabatan struktural Eselon IV dan III di Dinas Kebakaran DKI Jakarta. Berdasarkan latar belakang di atas dan
Keyword: Jabatan Struktural, Dinas Kebakaran