Judul: Introduksi Gen Osdep1 ke dalam Tanaman Padi Varietas Ciherang, Nipponbare, dan Kasalath melalui Perantara Agrobacterium tumefaciens
Abstrak: Development of high yielding rice varieties can be performed through Agrobacterium-mediated transformation techniques by inserting a gene that regulates the characters related to high yield. DEP1 gene (dense and erect panicle1) is a gene with single locus responsible for pleiotropy manner of three important traits i.e. dense panicle, grain number per panicle and erect panicle. The objective of this research was to introduce an Osdep1 gene into genome rice cv. Nipponbare, Ciherang, and Kasalath by using Agrobacterium-mediated transformation to obtain transgenic rice carrying the transgene. The vector used in this research was Agrobacterium tumefaciens strain LBA-4404. Concentration of higromycin antibiotic used for selecting agent was 50 mg/L, and acetosyringone compound was added to the media that is equal to 100 μM. In vitro Agrobacterium tumefaciens (OD600 = 0.3)-mediated transformation through dropping the bacteria to rice immature embryo explants have been successfully performed and produced three transgenic plants of Nipponbare. Meanwhile, in planta transformation have produced two transgenic plants cv. Ciherang and three transgenic plants cv. Kasalath. Inheritance analysis of T1 generation Kasalath-Osdep1-4 dan Kasalath-Osdep1-8 lines showed that the transgenic lines were not chimeras. Molecular analysis should be performed to make sure that the T1 transgenic rice lines also carry the transgene.
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Judul: Transformasi Genetika Padi Indica dengan Gen OsMADS18 Menggunakan Agrobacterium Tumefaciens untuk Merakit Varietas Padi Umur Pendek
Abstrak: Rice is the staple food of more than half the world's human population. The need of rice each year continues to increase with increasing population. This study aimed to assemble a short-lived rice varieties by introducing OsMADS18 gene into the genome of plants using Agrobacterium tumefaciens. OsMADS18 gene is a short age gene which has been successfully constructed to pCAMBIA1301 vector under CaMV35S constitutive promoter. This is evidenced by the emergence of 12561 bp DNA band size, this measure is a combination among vector pCAMBIA 1301 (11819 bp) and OsMADS18 genes (742 bp). Then the vector pCAMBIA 1301 that contains OsMADS18, was used to transform Agrobacterium tumefaciens strain EHA105 through Heat-shock method. The success of transformation marked by the growth of the white colonies Agrobacterium tumefaciens on solid media LB, this media contained antibiotic, X-gal and IPTG. Then the bacteria are used to transforms 50 calli Indica rice by soaking the calli into colonies for 20 minutes. The calli moved to selection media that contains hygromycin. After 14 days, calli that resistant to hygromycin moved to regeneration media R3B grown for transgenic plant. 18 transformants plantlets were obtained. Gus-A successful test on the show with the appearance of blue color in leaf tissues. While PCR analysis of the 18 transgenic plantlets produced 742 bp sized bands.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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