Judul: Pemurnian Parsial Dan Karakterisasi Glukosa Oksidase dari Isolat Aspergillus niger (IPBCC.08.610)
Abstrak: The increasing application of enzymes in industry, healthcare and energy initiates production rise of enzymes from microorganisms, one of them are glucose oxidase from Aspergillus niger, which is now develop as enzymatic fuel cell. The aim of this study was to purified and characterized the enzyme glucose oxidase from Aspergillus niger (IPBCC.08.610). Partial purification using amonium sulfate precipitation and then followed by dialysis. The purification fold of dialysis enzyme was 6.17, specific activity enzyme was 43.17 Umg-1. In addition, the activity of glucose oxidase was stable at 25 oC. However, the result of ion stabilization of the enzyme showed that the activity of glucose oxidase reduced by the presence of Ag+ ion with relative activity was 4.8% in 1 mM and 2.1% in 5 mM. Thiourea inhibit the relative activity to 58.44% in 1 mM. On the other hand, the molecular weight of enzyme unit was estimated 160 kDa and there are 2 protein subunits of the glucose oxidase with approximately molecular weight 80 kDa. At the end, in the aspect of enzyme kinetics, glucose oxidase had Km and Vmaks value (glucose as a substrate) respectively 43 mM and 33.67 Umg-1
Keyword: partial purification, glucose oxidase, characterization, Aspergilus niger (IPBCC.08.610)

Judul: Pemurnian dan Karakterisasi Enzim Glukosa Oksidase dari Isolat Lokal Aspergillus niger (IPBCC.08.610)
Abstrak: Production of various enzymes using microorganisms is one way of developing microorganism biodiversity in Indonesia. For this study, glucose oxidase (GOD) which is widely available for use and purchase in the commercial market was used. This research intended to determine the optimum incubation time for the production of GOD from Aspergillus niger local isolate (IPBCC.08.610) and determine the specific activity of the crude extract and purified enzymes fraction. In addition, it sought to determine the optimum temperature and pH level in GOD reaction and establish the values of Km and vmax. Optimum incubation time for GOD production of A. niger (IPBCC.08.610) was 48 hours with initial velocity of the reaction and the specific activity at 0.80/minute and 3.21 U/mg. Ammonium sulfate with 80% saturation was optimum to precipitate the GOD protein with specific activity of the fraction at 22.18 U/mg and purity levels at 6.92 times. Specific activity of the fraction of dialysis was 21.25 U/mg and the level of purity was 6.63 times. Km and vmax values were 46 mM and 11 U/mg with optimal temperature and pH level for GOD reaction between 37 - 45°C and pH 5.5.
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Judul: Potensi Dampak Pelarangan Cantrang Ditinjau dari Segi Perekonomian Masyarakat di PPP Tasikagung Rembang
Abstrak: Cantrang merupakan salah satu jenis alat tangkap yang termasuk pukat tarik dan dioperasikan di dasar perairan. Cantrang terdiri dari empat bagian utama yaitu, kantong, badan, sayap, dan mulut. Di sisi lain, cantrang merupakan alat penangkapan ikan tradisional yang sebagian besar digunakan oleh nelayan Pantai Utara Pulau Jawa, sehingga pelarangan cantrang akan menimbulkan dampak bagi kehidupan nelayan. Dampak ekologis pelarangan cantrang akan menimbulkan dampak positif bagi kondisi lingkungan, namun kenyataan tersebut akan berbanding terbalik dengan dampak ekonomi dan sosial yang ditimbulkan. Pada aspek ekonomi, pelarangan cantrang akan memengaruhi tingkat pendapatan, jumlah hasil tangkapan, dan diferensiasi alat tangkap. Peraturan Menteri Kelautan dan Perikanan Nomor 2 Tahun 2015 sebagaimana telah dicabut oleh Peraturan Menteri Kelautan dan Perikanan Nomor 71 Tahun 2016 yang menegaskan bahwa alat tangkap cantrang dilarang pengoperasiannya di semua jalur penangkapan ikan di seluruh WPPNRI.
Keyword: Cantrang, dampak ekonomi, pelarangan, peraturan