Judul: Deteksi dan Kloning Gen Inhibitor α-Glukosidase Streptomyces sp. BWA 65 serta Potensinya sebagai Anti Hiperglikemik pada Mencit (Mus musculus)
Abstrak: Diabetes mellitus is a metabolic disorder characterized by the presence of hyperglycemia due to defective insulin secretion, defective insulin action or both. One therapeutic approach for treating diabetes is to decrease the post-prandial hyperglycemia. This is done by preventing the absorption of glucose through the inhibition of the carbohydrate-hydrolysing enzymes α-glucosidase and α-amylase in the digestive tract. Actinomycetes have been known as source of commercialized acarbose, an α-glucosidase inhibitor. Acarbose is keto analog moieties of the C7N aminocyclitols. Sedoheptulose 7-phosphate is converted into 2-epi-5-epi-valiolone via the activity of sedoheptulose 7-phosphate cyclase, at the first step of the biosynthesis of C7N aminocyclitol. This research aimed to detect and clone sedoheptulose 7-phosphate cyclase gene and to investigate the capability of crude α-glucosidase extract from Streptomyces sp. BWA 65 in lowering blood glucose levels in mice. Detection of sedoheptulose 7-phosphate cyclase gene was done by using Polymerase Chain Reaction (PCR) with designed primers C7N aminocyclitol. The primer used was designed on the basis of the known sequence of sedoheptulose 7-phosphate cyclase (acbC) from that of Actinoplanes sp. SE50/100 which was then cloned by T-Vector pMD20. The in vivo experiment was conducted by using thirty mice, by the oral glucose tolerance test (OGTT) and induction of streptozotocin diabetes methods. The result showed that there was similarity of nucleotide series sedoheptulose 7-phosphate cyclase of Streptomyces sp. BWA 65 wich have 100 % sequence similiarity with DNA fragment of sedoheptulose 7-phosphate cyclase Actinoplanes sp. complete acarbose (acb) gene cluster, strain SE50/110, accses number Y18523.4 reported in the GenBank analysis through Blast Program. The result indicated that the designed primer was able to amplify the sedoheptulose 7-phosphate cyclase acarbose although the accomplishment in amplifying the gene was still up to 300 bp. The result for in vivo experiment by oral glucose tolerance test (OGTT) showed that α-glucosidase extract from Streptomyces sp. BWA 65 had potency about 75 percent in decreasing blood glucose levels postprandial compare to acarbose and for induction of streptozotocin diabetes showed that α-glucosidase extract from Streptomyces sp. BWA 65 had an effect in decreasing blood glucose levels diabetic mice hyperglycemia better than the acarbose concentration examined. The in vivo experiment indicated that α-glucosidase inhibitor from Streptomyces sp. BWA 65 had potential as antidiabetic in mice.
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Judul: Aktivitas Inhibitor Alpha Glukosidase Streptomyces sp. BWA 65 pada beberapa Media, pH, dan Suhu
Abstrak: Streptomyces sp. BWA 65 is a genus of actinomycetes from brotowali (Tinospora crispa), which produces α-glucosidase inhibitor as antidiabetic. Production of this compound is influenced by external factors such as media, pH, and temperature. The α-glucosidase inhibitor activity was assessed at various media, pH, and temperature. Streptomyces sp. BWA 65 grew and sporulated well on YSA medium, indicated by the formation of white colonies after incubation for 7-10 days. ISP-2 medium was the best growth medium compared with YS and ISP-4 media. In this ISP-2 medium, Streptomyces sp. BWA 65 produced optimum inhibition of it’s supernatant and it’s ethyl acetate extract after 10 days of production time. This optimum growth condition was used for the production of α-glucosidase inhibitor compound using a fermentor at control condition, e.g. 27- 29 °C, pH 7, and oxygen pressure at 3-5 mmHg. The α-glucosidase inhibitor compound produced was assayed at various pH and temperature. The higest percentage of inhibitor activity of supernatant and ethyl acetate extract was found at pH 7, 27 °C for 24 h incubation. At this condition, the highest percentage of inhibition of the supernatant was 90.2 %, while for ethyl acetate extract was 91.8 %. As a comparison, acarbose at 1000 ppm concentration gave 98,6 % inhibition activity.
Keyword: Bogor Agricultural University (IPB), temperature, Streptomyces sp. BWA 65, pH, medium, α-glukosidase inhibitor activity

Judul: Distribusi suhu, salinitas dan oksigen terlarut di Laut Arafura Selatan pada bulan November 1992
Abstrak: Tujuan penelitian ini adalah (1) mengetahui variabilitas sebaran suhu, salinitas dan oksigen terlarut secara mendatar dan menegak, (2) Mengidentifikasi massa air di Laut Arafura Selatan. Data yang digunakan dalam penelitian ini adalah data Ekspedisi MINA ZEE-06 1992 pada bulan November 1992. Daerah yang diteliti adalah perairan Laut Arafura bagian selatan yang termasuk Zona Ekonomi Eksklusif Indonesia (ΖΕΕΙ). Suhu dan konduktivitas air laut diukur dengan menggu- nakan CTD Guildline 6000. Nilai suhu ditentukan dengan persamaan salinitas dan konduktivitas. Pengukuran dilakukan di 18 stasiun oseanografi dari permukaan hingga dekat dasar. Sampel air laut untuk menentukan kadar oksigen terlarut diambil dengan botol Niskin pada kedalaman "stan- dar". Kadar oksigen terlarut dianalisa dengan menggunakan metode Winkler. Data menunjukkan bahwa suhu lapisan permukaan berkisar antara 27.72 28.47 °C dengan rata-rata 28.10 °C, dan nilai yang lebih tinggi terdapat di bagian barat. Sebaran suhu secara mendatar memperlihatkan adanya kecenderungan suhu lebih tinggi di daerah barat.
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