Judul: Perkecambahan Embrio Endospermik Mangga (Mangifera indica L.) Varietas Arumanis Klon 143 secara In Vitro
Abstrak: This study aimed to obtain the optimal medium for germination of endospermical embryos in mango (Mangifera indica L.) cv Arumanis clone 143. The effect of cold treatment and GA3 treatment had no significant difference in endospermical embryos germination. In the first experiment, embryos with 8 days cold treatment 83.3% of cotiledonary embryos produced the secondary proembryos with 33 secondary embryos. In the second experiment, the highest percentage of secondary proembryos (83.3%) we obtain in MS medium with 5 μM GA3. In the third experiment, the solid MS medium with 5 mg/l BA produced 80 secondary embryos, highest than in liquid medium with the similar composition, and 6.7% of that embryos germinated on solid medium. In the fourth experiment, the embryo medium induction has significant difference effect in embryo which produced by inoculum, whereas preculture medium had no significant difference effect. Inoculum from MS medium with 10 μM GA3 and cultured on PM preculture medium (MS medium with 5 mg/l 2,4-D, 5 mg/l GA3, 400 mg/l amino acid glutamine, 300 mg/l thrypton and 200 ml/l coconut water) produced embryo at 25.67 in 7 MST. The highest percentage of germinated embryos (32.4%) produced by inoculum from medium containing MS halfstrength major and minor nutrients with 5% coconut water, and then cultured on PM preculture medium.
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Judul: Induksi embriogenesis in vitro sel endosperma mangga (Mangifera indica L.) varietas arumanis klon 143
Abstrak: Mango Arumanis (Mangifera indica L) is a diploid plant with 2n=2x=40 chromosome. Embryogenesis from endosperm cells is the one opportunity to produce seedless triploid plant in mango Arumanis. The objective of this research are to obtain the optimum age of endosperm of mango seed for embryogenesis induction and to obtain the optimum medium for embryogenesis induction. This research conducted with 3 experiment are : (1) embryogenic callus induction from endosperm cells; (2) callus proliferation and (3) maturation of somatic embryo. Endosperm cells from different age of immature seed are : 1 week old, 2 weeks old and 3 weeks old from the initial fruit set was cultured in 10 medium compotition with several plant growth regulators and organic compound to induce embryogenic calli. Result of this research are : (1) Three weeks old endosperm cells from immature seed showed the best results for callus induction with percentage 26.28%, (2) Medium MA3 (B5 macro + MS micro + 0,2 mg/l BAP + 1 mg/l NAA + 0,5 mg/l GA3 + 3% sucrose) was the best medium for callus induction and somatic embryo development.
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Judul: Formulasi TSP (Traveling Salesman Problem) menggunakan ILP (Integer Linear Programming)
Abstrak: TSP is a journey of salesman f?om his origin town (place) to visit n-cities (places) only at once, then return to his origin town (place) with a minimum total distance. Its purpose is to minimize the operating expense that is released by the company. Vehicle route at TSP in the form of a Hamiltonian cycle, that is closed path containing all nodes at the graph which representing a network walk. Its problem is to determine a journey route which is feasible (possibly can be passed by) in such a way the vehicle travelled distance passed the route is minimum.
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