Judul: In Vitro Stress Treatment On Axillary Shoots of Mucuna bracteata to Increase Shoot Number
Abstrak: Mucuna bracteata is a plant that is very effectively used as a legume cover crop (LCC), which is a cover crop plant that have a function to suppress weeds growth. Productivity of M. bracteata shoots in tissue culture have an effect in the plantation industry. However, at a certain subculture from the multiplication medium to the next subculture, there is a decline in shoot growth of M. bracteata. This study aims is to increase the number of shoots of M. Bracteata with stress methods of culture induction of shoots. Medium stress treatments performed for 14 days in the dark and bright with the composition of medium MS macro 0-25% (A), MS macro 25-50% (B), MS macro 50-75% (C), sucrose 50-75% (D), and vitamins 50-75% (E). After observation for two months, the number of shoots is same between sample with the media's treatment of (A), (B), and (E), even the number is decreasing 100%. That is unlike the (C) which the number of shoots is increase. Similar thing occurs in (D) treatment, although it is not as good as (C). Differences of storage space affect the morphology of the explants. Explants were incubated in the dark room similar to the sprouts, while the explants were incubated in the light have a morphology that similar to the original plant. This shows that the best media of stress treatment used is a composition of MS macro 0-25% (C) in bright conditions.
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Judul: In Vitro Shoot Induction of Kaempferia parviflora Wall. Ex. Baker
Abstrak: This research aimed to find the best MS salt concentration and cytokinin (BAP) concentration for K. parviflora rapid multiplication by using in vitro buds as explants. The experiment was done at Plant Tissue Culture Laboratory of Agronomy and Horticulture Department, Faculty of Agriculture, Bogor Agricultural University from January to November 2011. This research began by sterilizing shoots of K. parviflora rhizomes received from PT. Ogawa Indonesia to produce axenic culture. After about 8 weeks of growth, young shoots began to sprout. The young shoots of K. parviflora axenic culture were then cut and cultured individually on the treatment medium, which consist of two different MS salt concentrations (MS and ½ MS) and five different 6-benzylaminopurine (BAP) concentrations (0, 1.25, 2.5, 3.75, and 5 ppm) respectively. Explants started to grow after 2 weeks of culture. Both MS salt concentration and BAP concentration treatments did not give a significant effect on the first shoot induction time, but ½ MS medium supplemented with 5 ppm BAP could induce first shoot faster than other treatments, which is within an average of 2.5 weeks after cultured (WAC). At early observation (2 to 4 WAC), medium enriched with 3.75 ppm BAP induced more shoot than the other treatments, but at the end, control medium (MS0) induced more shoot than other treatments. Starting from 2 WAC, the explants cultured on 0 ppm BAP medium grow more rapidly than the other treatments, but after 10 WAC, they grow slower. All treatments have the same trend of leaf growth. At 2 to 8 WAC, the number of leaves is rising rapidly, but at the 9 WAC, it began to slow down.
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Judul: Infeksi Virus Parainfluenza Pada Anjing
Abstrak: Virus parainfluenza anjing termasuk famili Paramyxoviridae, berbentuk pleomorf dengan ukuran bervariasi (bergaris tengah antara 100-500 nm), berpembungkus peka ether, berisi genom RNA dan secara serologis mempunyai kesamaan dengan virus SV-5 kera; dikenak sebagai salah satu kausa penting dari sindrom "kennel cough". Infeksinya dapat mengenai anjing semua umur yang belum pernah terinfeksi atau mendapatkan vaksinasi mengakibatkan gejala klinis ringan yang terbatas pada traktus respirasi atu infeksi yang secara klinis tidak memperlihatkan gejala nyata. Gejala klinis yang nampak setelah masa inkubasi (1-5 hari) terlewati berupa batuk yang tidak berat selama beberapa hari, berbagai tingkat tonsilitis dan pharyngitis dan kadang-kadang ditemukan kenaikan temperatur rektal antara 1-2 derajat C dari temperatur normal rat-rata. Anjing terinfeksi mengandung virus pada traktus respirasi bagian atas dan bawah serta limfonoduli sampai hari ke 9 setelah infeksi. Penyebaran virus antar anjing terjadi secara aerogen.
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