Judul: Pengaruh leverage terhadap profitabilitas pada PT Mitra Niaga Sentratama
Abstrak: Sebuah perusahaan harus mengantisipasi agar tetap mempertahankan eksistensinya di masyarakat yaitu bisa dengan cara diversifikasi usaha. Untuk melakukan diversifikasi usaha dibutuhkan struktur pendanaan yang tepat seperti seberapa banyak modal yang diperlukan, bagaimana rencana alokasi modal dan darimana dana tersebut diperoleh. Tujuan penelitian ini adalah untuk mengetahui struktur modal perusahaan sebelum dan sesudah menjadi PT, mengetahui tingkat cost of capital yang timbul dari penggunaan utang dan mengetahui tingkat keefektifan leverage dari utang yang digunakan. Penelitian ini menggunakan laporan keuangan PT. Mitra Niaga Sentratama tahun 2004 – 2013 dengan menggunakan alat analisis rasio keuangan diantaranya rasio solvabilitas (DR dan DER), rasio profitabilitas (ROI dan Profit Margin) dan WACC (Weighted Average Cost of Capital). Hasil analisis menunjukkan struktur modal sebelum PT menunjukkan tingkat utang jangka panjang sebesar 1,2% dan setelah PT sebesar 10,3% dengan biaya modal yang timbul adalah sebesar 15,48%. Hasil analisis regresi menunjukkan nilai R square 0,004 yang artinya tingkat Leverage tidak berpengaruh terhadap profitabilitas.
Keyword: capital structure, cost of capital, leverage

Judul: Pengaruh leverage terhadap likuiditas dan profitabilitas pada perusahaan pertambangan menurut indeks kompas 100 periode 2010-2013
Abstrak: Pendanaan menjadi masalah penting bagi perusahaan perusahaan. Pendanaan melalui utang akan mempengaruhi aset dan laba perusahaan. Penelitian ini bertujuan untuk me ngetahui kondisi struktur modal perusahaan pertambangan dan pengaruh dari leverage terhadap likuiditas dan profitabilitas pada perusahaan pertambangan menurut Indeks Kompas 100 periode 2010 2010-2013. Metode yang digunakan dalam penelitian adalah Structural Equ ation Modeling menggunakan alternatif Partial Least Square. Hasil penelitian adalah bahwa perusahaan tambang menggunakan modal internal yang lebih besar daripada utang untuk sumber pendanaannya yang berarti perusahaan masih mampu membiayai aktivitas operas ionalnya sendiri. Dari pengujian statistik, diketahui bahwa pengaruh leverage adalah negatif dan signifikan baik terhadap likuiditas maupun profitabilitas dengan nilai R-Square pada variabel likuiditas sebesar 0,427 dan pada variabel profitabilitas sebesar 0,426 yang berarti bahwa 43% variabilitas pada likuiditas dan 43% variabilitas pada profitabilitas dapat dijelaskan oleh leverage.
Keyword: leverage, liquidity, profitability

Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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