Judul: Kajian morfometrik cumi-cumi sirip besar (Sepioteuthis lessoniana, Lesson) di Perairan Teluk Jakarta
Abstrak: Salah satu jenis cumi-cumi mempunyai nilai ekonomis penting adalah cumi-cumi sirip besar (Sepioteuthis lessoniana, Lesson) dan merupakan salah satu anggota Famili Lolinginidae. Cumi-cumi sirip esar ditemukan di seluruh perairan Indonesia dan seluruh produksinya merupakan hasil tangkapan. Studi mengenai morfometrik dari cumi-cumi sirip besar sangat diperlukan huna selektivitas ukuran bagi kegiatan penangkapan. Selektivitas ini sangat penting dilakuakn untuk menghindari terjadinya overfishing cumi-cumi sirip besar hidup di daerah lepas pantai, terumbu karang, daerah dekat pantai dan estuaria.
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Judul: Studi Perkembaogan Kapsul Telur dan Embrio Cumicumi Sinp Besar (Sepioteuthis lessoniana, Lesson) Pada Berbagai Tingkat Salinitas.
Abstrak: Cumi-cumi sirip besar (Sepioteuthis lessoniana, Lesson), merupakan salah salu jenis dari kelas Cephalopoda yang memiliki nilai ekonomis penting karena merupakan salah satu sumber makanan laut yang memiliki nilai gizi yang baik karena mengandung asam amino essensial yang dibutuhkan oleh tubuh manusia. Sebagian besar produksi cumi-cumi sirip besar di Indonesia berasal dari tangkapan di alam dan jika hal ini dilakukan terus-menerus tanpa memperhatikan kelestariannya maka penurunan populasi bahkan kepunahan akan segera terjadi. Populasi cumi-cumi di laut semakin menurun sehingga diperlukan penelitian untuk mengantisipasi punahnya cumi-cumi. Budidaya cumi-cumi merupakan salah satu alternatif dalam memenuhi kebutuhan lokal maupun ekspor dengan mengetahui beberapa aspek dari sistem pembenihan cumi-cumi sirip besar. Tujuan dilakukan penelitian ini adalah untuk mengetahui perkembangan kapsul telur dan embrio pada tingkat salinitas berbeda yang diharapkan dapat memberikan tambahan informasi untuk meningkatkan kualitas dan kuantitas benih cumi-cumi sirip besar.
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Judul: Glucose biosensor using a glucose dehydrogenase flavin adenine dinucleotide electrochemically immobilized on zeolite nanoparticles
Abstrak: A glucose biosensor was developed to measure blood glucose levels. The performance of these new glucose biosensors was continuously improved, producing better performance and stability. The technique selection and immobilization matrix are key factors for biosensor development, so that the prospecting of materials that can be used as a matrix for supporting enzyme, continue to be explore. The purpose of this study was to determine the activity and stability of the enzyme glucose dehydrogenase (GDH) that were immobilized on zeolite nanoparticles (NPZ) and determine the kinetic parameters and analytical performance by electrochemical methods. The resulting enzyme electrode with NPZ and enzyme electrode with zeolite A showed a high activity against the enzyme electrode glucose than without NPZ. The reaction kinetics of third electrodes of enzyme followed the Dixon equation. The 𝐼����𝑚����𝑎����𝑥����𝑎����𝑝����𝑝���� values for enzyme electrode without NPZ, with NPZ, and with zeolite A were 27.25 μA, 77.52 μA, and 48.54 μA, respectively. The 𝐾����𝑀����𝑎����𝑝����𝑝���� values for GDH enzyme that immobilized on the NPZ were 1.16 mM, 12.30 mM for GDH enzyme that immobilized on zeolite A and 0.95 mM for GDH enzyme immobilization without NPZ. The enzyme electrode without NPZ, with NPZ, and with zeolite A also showed a high sensitivity with value for the sensitivity were 0.58 μA mM-1, 0.81 μA mM-1, and 2.51 μA mM-1, respectively. The third enzyme electrodes showed different linear area with range for the enzyme electrode without NPZ, with NPZ and with zeolite A were 1.00-15.00 mM, 2.00-20.00 mM, and 0.06-10.00 mM, respectively. The blood glucose level of normal person ranged from 4 to 6 mM, so that the enzyme electrode with NPZ and without NPZ are suitable for use in the determination of the concentration of blood glucose. While the enzyme electrode with zeolite A has a smaller range so it is more suitable to use in the determination of glucose concentration in the foods or beverages. The enzyme electrode with NPZ and the enzyme electrode with zeolite A showed a good accuracy with relative error values were 3.84% and3.48%, respectively. Repeatability measurements also showed a good precission results with relative standard deviation values (%RSD) for the enzyme electrode with NPZ and enzyme electrode with zeolite A were 3.83% and 3.16%, respectively. By combining two immobilization techniques, physical adsorption on zeolite and entrapment on carbon paste, high enzymatic activity, good accuracy, and repeatability was attained. The enzyme electrode with NPZ and the enzyme electrode with zeolite A demonstrated a good electrochemical response toward glucose, allowing it to detect blood glucose levels and glucose levels in foods and beverages.
Keyword: glucose biosensor, immobilization, zeolite