Judul: IPB (Bogor Agricultural University)
Abstrak: The Indonesia population growth rate during the past 10 years tend to decrease and nearly reach the ideal rate of one percent of growth per year. This success was supported by family planning program and campaign of using contraception. Unfortunately, almost all contraceptive tools and methods affect women. Various effort needs to be done by promoting men contraception, in order to increase participatory rate of men. Medicinal plants have been used as alternative medicine with an asumption that it perform less side effects, beside other consideration such as easy to find and cheaper than the chemical and commercial medicines. One of the medicinal plants that have been used widely in the community is pegagan (Centella asiatica) that used to treat injuries and improve cognitive function. Pegagan has been reported to have an antifertility properties in male rats. However, the scientific reports on the use of this plant on reproduction purposes are still limited. So that, the purpose of this study is to determine the antifertility effect of pegagan extract and reversibility of reproductive capability in male rats. In the first stage, this experiment was done experimentally with a completely randomized design, consisting of 16 combinations of four treatment doses (0, 50, 150 and 450 mg/BW) and four duration of administration (28, 35, 42 and 49 days). Parameters measured were population of spermatogenic cells, sperm quality and testosterone levels. Results of the experiment showed that the population of late spermatids, sperm concentration, sperm motility and testosterone levels decreased following the treatment, although they were still in a normal range. Results of the experiment also indicated that pegagan extract did not affect the population of spermatogonia, primary spermatocytes and sperm abnormality. It also showed that pegagan still be able to maintain testosterone levels within the normal range. Based on these results, it can be concluded that the dose of 450 mg/kg BW caused the lowest level of late spermatids and sperm concentration if compared to the other paramaters. Twenty eight days administration of pegagan appears to be most efficient duration of administration than any longer time. Results of the experiment also indicated that treatment doses of 450 mg/kg BW and duration of 28 days is still able to maintain testosterone levels in the normal range. Decrease of testosterone levels is associated with inhibition of late stages of spermatogenesis. Based on these results, follow up experiment was carried out to explore the antifertility effects of pegagan and its reversibility. The study was conducted with a complete randomized design, with 8 treatment of combination of dose-duration (450 mg/kg BW-21 day, 600 mg/kg BW-21 day, 450 mg/kg BW-28 days, and 600 mg/kg BW-28 days) and the cessation length of pegagan (1 day and 12 days). Several parameters consisting of the degree of spermatogenesis and sperm quality of male rats were determined. Results of the experiment showed that the degree of spermatogenesis and sperm quality decreased after administration of pegagan. The decrease of spermatogenesis may be due to apoptosis process. The results also showed that 12 days cessation length of pegagan cause the increase of developmental stage, quality
Keyword: antifertility, reproductive function, pegagan, rat

Judul: IPB (Bogor Agricultural University)
Abstrak: Pasak bumi is popular in the traditional medical herb that is believed can increase the secondary reproduction characters such as: 1. libido and 2. the duration of erection. Here a reported the research that focus on: 1. sexual behavior of the male white rat treated by pasak bumi. and the better dose of pasak bumi that indicated highest libido. 2. mechanism of pasak bumi in the hypophysis cells that produce reproduction hormones including: acidophile and basophile cells distribution, the response of luteinizing hormone (LH) cells and the response of follicle stimulating hormone(FSH) cells. 3. The works of pasak bumi in the production of testosteron 4. The works of pasak bumi in the development stage of spermatogenesis in the seminiferous tubule. 5. The works of pasak bumi in the quality of semen in cauda epididymis including profile of cauda epididymis ductus diameter, some parameters of macroscopical and microscopical semen quality. Research carried out in five experiments; experiment 1. using the designed cage to observe the male white rat behavior seduced by female oestrus in separating wire net. Then, the male white rat administered orally by various doses of pasak bumi to find out the best dose. Experiment 2, using HE staining method to study micromorphological distribution of the acidophile and basophile cells in hypophysis, and using immunohistochemical staining method to study the response of LH cells of the male rat hypophysis treated by pasak bumi, also using immunohistochemical staining method to study the response of FSH cells. Experiment 3.using the RIA method to measure testosterone level in blood serum. Experiment 4.using HE staining method to study micromorphological of the profile of spermatogenesis stage in the seminiferous tubule. Experiment 5.using HE staining method to study micromorphological of the diameter of cauda epididymis, also doing macroscopical and microscopical semen quality in the cauda epididymis. Result of the research showed that: 1. Sexual behavior that reflected the male white rat libido were: a. face to face meeting, b. moving close to wire net partition, c. scratching the wire net partition. 2.The optimal dose of pasak bumi resulted highest libido was 18 mg/200 g bw (body weight) compared with 100 mg/200 g bw, 200 mg/200 g bw, and the control (1 ml aquadest). 3. Basophyle cells increased significantly in the third day of pasak bumi administration; LH cells strongly significantly response to produce LH hormone within cells in the third day; FSH cells weakly response (non significantly) to produce FSH hormone within cells in the third day. 4. The level of testosterone in plasma increased significantly in the third day of pasak bumi administration. 5. The last spermatid actively increased in the third day. 6. The quality of semen in the cauda of epididymis increased significantly in the third day of pasak bumi administration.
Keyword: pasak bumi, libido, LH cells, FSH cells, testosterone, semen quality

Judul: Formulasi Hamiltonian untuk Menggambarkan Gerak Gelombang Soliter Dimensi Tiga di Permukaan Laut
Abstrak: Gelombang permukaan adalah gelombang yang terjadi perbedaan rapat massa antara air dan udara. Salah satu gelombang permukaan yang ditinjau adalah gelombang soliter. Formulasi gelombang soliter dilakukan dengan menggunakan formulasi Hamiltonian.
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