Judul: Hubungan Antara Pola Menonton Sinetron Dengan Gaya Hidup Hedonisme Remaja Pedesaan
Abstrak: Sinetron merupakan salah satu hiburan bagi masyarakat yang disiarkan melalui televisi. Namun peningkatan minat masyarakat khususnya remaja terhadap sinetron tidak diimbangi dengan peningkatan kualitas dari sinetron tersebut. Kualitas sinetron yang buruk dikhawatirkan memberikan perubahan gaya hidup bagi remaja. Penelitian ini menggunakan metode survei dengan pendekatan kuantitatif menggunakan instrumen kuesioner yang didukung dengan data kualitatif, yaitu wawancara mendalam. Jumlah responden pada penelitian ini adalah 50 orang remaja Desa Benteng. Pada penelitian ini ditemukan bahwa tingkat perhatian menonton sinetron mempunyai hubungan dengan aktivitas gaya hidup hedonisme. Hubungan nyata lainnya adalah antara tingkat ekonomi keluarga dengan opini dan aktivitas gaya hidup hedonisme dan hubungan antara tingkat interaksi dengan keluarga dengan aktivitas gaya hidup hedonisme.
Keyword: hedonisme, remaja, sinetron

Judul: Pola menonton sinetron dan perilaku etis remaja : kasus sinetron bertemakan remaja di televisi
Abstrak: Pads bulan Maret 2006, sebanyak 35 persen dari sinetron yang ditayangkan di televisi adalah sinetron batemakan remaja. Sebagian besar sinetron tersebut memiliki rating yang tinggi Banyaknya jumlah sinetron bertemakan remaja dengan rating yang tinggi tersebut menyebabkan kekhawatiran banyak pihak mengenai dampak yang akan dialami oleh remaja karena di dalam sinetron bertemakan remaja banyak terkandung muatan negatif dan sering menampilkan adegan-adegan anti sosial. Melihat kenyataan tersebut, maka dilakukan penelitian dengan tujuan sebagai berikut : 1) Mengungkap pola menonton sinetron bertemakan remaja pada remaja yang tinggal di kota dan di desa; 2) Mendeskripsikan faktor-faktor yang mempengaruhi perilaku remaja yang tinggal di desa maupun di kota dalam menonton sinetron bertemakan remaja; 3) Mengetahui pengaruh menonton sinetron bertemakan remaja terhadap kesesuaian periilaku remaja dengan etika pergaulan pada remaja yang tinggal di desa maupun di kota.
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Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable