Judul: Variasi Sensila pada Antena Ngengat Scirpophaga incertulas Walker (Lepidoptera: Crambidae)
Abstrak: Scirpophaga incertulas Walker (Lepidoptera: Crambidae) moth infests paddy, therefore it is known as yellow rice stem borer. The antenna of moth S. incertulas serve as the olfactory organ. The pores in antennal sensilla allow the feromon pass to the inner side of the antenna. The aim of this research was to study the diversity of antennal sensilla from the male and female S. incertulas by using scanning electron microscope (SEM). Goldplating method was applied for the purpose of coating process to increase the slide contrast. The antenna S. incertulas is filiform type and the antenna consists of three main parts, i.e. scape, pedicle, and flagellum. This study found that the S. incertulas male antenna has two types of sensila: trichodea sensilla (having three variations: TrI, TrII, and TrIII types) and bassiconica sensilla with two variations, BaII and BaIII types. Further in female antennal, the result of this study revelaed four types of sensilla: trichodea sensilla (TrI, TrII, and TRIII types), bassiconica sensilla (BaIII type), chaetica sensilla, and three variations of coeloconica sensilla (CoI, CoII, and CoIII types).
Keyword: Trichodea, Yellow rice stem borer, Sensila, Scanning Electron Microscope (SEM), Chaetica, Bassiconica

Judul: Identification of Pheromone Binding Protein Gene of Yellow Rice Stem Borer Scirpophaga Incertulas (Walker) (Lepidoptera: Crambidae)
Abstrak: The yellow rice stem borers (YRSB) moth, Scirpophaga incertulas (Walker) (Lepidoptera: Crambidae), is known as the most major rice stem borer in tropical Asia. Pesticides are not effective to control the population of these insects due to almost entire larvae phase and pupae are in the rice stem. Hence, other control technique is needed such as based on the mating behaviour. Pheromone binding protein (PBP) in male S. incertulas antennae plays a role in the recognition of sex pheromone produced by the female, therefore influenced in their mating behavior. The aim of this study was to identify PBP gene of S. incertulas. For the purpose to collect imagoes as DNA source, S. incertulas were reared. Touchdown PCR and touchdown-nested PCR were the main techniques conducted to identify genomic of PBP gene from S. incertulas and revealed 700 and 600 bp amplicons, respectively. Those amplicons strongly expected as PBP gene. Sequence analysis of S. incertulas from touchdown-nested amplicon identified 575 bp which was consisted of 169 bp of exon 3 and 406 bp of intron 2. This study revealed putative amino acid sequences of exon 3 from S. incertulas has one conserved cysteine while other Lepidopterans PBP have three conserved cysteine. In phylogenetic analysis, the putative amino acid sequences obtained, showed a phylogenetic signal i.e. by clustering with PBPs from other Crambidae moths. The result of this study is important as a basic data for PBP expression analysis in female or male S. incertulas as the initial step to develope new insect biocontrol.
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Judul: Perencanan Lanskap Sunda Kalapa, Jakarta Utara Sebagai Kawasan W isata Sejarah
Abstrak: Perkembangan kota Jakarta dari waktu ke waktu sangatlah cepat, sehingga menyebabkan kota-kota tua banyak yang tergusur untuk dijadikan daerah baru yang lebih modern. Apabila hal tersebut tidak ditanggulangi secara dini maka keberadaan Kota Lama Jakarta akan hilang. Hal inilah yang melatar belakang untuk dilakukan tindakan perlindungan dan revitalisasi kawasan bersejarah Sunda Kalapa.
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