Judul: Identifikasi Keragaman Gen Alpha-S1 Casein g.12164G>A pada Kambing Perah di Balitnak
Abstrak: Polimorfisme gen Alpha-S1 Casein (CSN1S1) berimplikasi pada keragaman komposisi susu dan kualitas curd keju. Penelitian dilakukan untuk mengidentifikasi keragaman gen CSN1S1 g.12164G>A, berasal dari 67 set DNA terdiri dari kambing PE 5 ekor, saanen 8 ekor, Sapera 51 ekor, dan SaanPE 3 ekor di Balitnak. Polimorfisme diidentifikasi menggunakan PCR-RFLP enzim DdeI standar Phenol-chloroform. Keragaman gen dianalisis menggunakan program Popgen32. Amplifikasi menghasilkan panjang fragmen 438 bp. Enzim DdeI memotong produk PCR menjadi 3 fragmen, yakni 192 pb, 145 pb, dan 101 pb, dinyatakan sebagai alel G, sedangkan alel A apabila terpotong menjadi 2 fragmen, yaitu 337 pb dan 101 pb. Frekuensi alel G tertinggi terdapat pada kambing saanen (0.625), Sapera (0.578), PE (0.400) dan terendah SaanPE (0.333). Frekuensi alel A tertinggi terdapat pada kambing SaanPE (0.667), PE (0.600), Sapera (0.422) dan terendah pada kambing saanen (0.375). Keragamaan tertinggi terdapat pada kambing saanen (0.75). Sebagian besar kambing perah pengamatan berada dalam kesetimbangan Hardy-Weinberg, terkecuali kambing SaanPE. Berdasarkan data keragaman, dapat disimpulkan bahwa kambing perah di Balitnak Ciawi bersifat polimorfik untuk gen CSN1S1 g.12164G>A
Keyword: gen CSN1S1, kambing perah, PCR-RFLP, polimorfisme

Judul: Identifikasi keragaman gen β-Kasein (CSN2) pada kambing peranakan etawah, saanen dan persilangannya dengan metode PCR-SSCP
Abstrak: Casein genetic polymorphisms are important and well known due to their effects on quantitative traits and properties of milk. β-kasein gene is directly related to the quality and properties of milk. A protocol for the rapid and simultaneous genotyping of β-kasein alleles was conducted by single strand conformational polymorphism polymerase chain reaction (SSCP-PCR) method in goat. Screening β-kasein gene variability in 3 dairy goat breeds was conducted for Etawah Grade (77 samples), Saanen (67 samples) and PESA (Crossbreed Etawah Grade with Saanen) (29 samples) in Bogor and Sukabumi. The objective of this research was to identify polymorphism of the β-kasein (CSN2) gene in dairy goat. This research found three alleles of the β-casein gene, ie CSN2*A, CSN2*C, dan CSN2*O. In most breeds, CSN2*O occurred in the lowest frequency. The identification of the CSN2 gene variability in the goat breeds indicated the highly of the A allele. The CSN2*A allele had a high frequency in Saanen in Cijeruk (0,66); Etawah Grade in Cariu (0,62); and PESA in Cariu (0,54). While the CSN2*C allele had a high frequency in PESA in Balitnak (0,83); Etawah Grade in Ciapus (0,48); and Saanen in Taurus (0.38). Based on the results of chi-square analysis, found that Saanen in Cariu and Taurus were not in Hardy-Weinberg equilibrium.
Keyword: 

Judul: Utilization of terra/MODIS L1B data for analysis of horizontal wind profile in the troposphere
Abstrak: This study was concerned with horizontal wind derived from data collected with the Moderate-resolution Imaging Spectroradiometer (MODIS) on board the NASA’s Earth Observation System (EOS)-Terra satellite. The aim of this study is to develop a method for the estimation of horizontal wind movement in the troposphere based on Navier-Stokes equation. The method was applied to a calibrated and geolocated Terra/MODIS L1B 1 km data set. The data inputs to estimate surface temperature are retrieved from TIR (Thermal Infrared) bands 31 (10.780 μm to 11.280 μm) and 32 (11.770 μm to 12.270 μm). The obtained surface temperatures were later used to approximate geopotential height on a constant-pressure surface by using the modified Poisson equation. The main assumptions used are the hydrostatic equilibrium, ideal gas law, constant lapse rate and constant acceleration of gravity. Based on the distribution of geopotential height, the pressure gradient force is measured by the gradient of geopotential on an isobaric coordinate to present the horizontal wind profile. For certain topographic areas especially in the mountain ranges, there was a disadvantage of using pressure as a vertical coordinate which lead to inaccuracy. In spite of that, Terra/MODIS L1B data with 1 km spatial resolution can be used to estimate the horizontal wind movement in the troposphere.
Keyword: