Judul: Spesific Antibody Response of Influenza amongst Layer Chickens Vaccinated with Influenza Quadrivalent
Abstrak: Virus influenza merupakan virus yang dapat menular dan menginfeksi saluran pernapasan, memiliki spektrum yang luas meliputi manusia dan beberapa spesies lainnya. Virus influenza yang sering menginfeksi manusia yaitu influenza A dan B. Penularan virus influenza antar manusia terjadi melalui droplet saat batuk, bersin, dan berbicara. Penelitian ini bertujuan untuk mempelajari respons pembentukan antibodi spesifik virus influenza pada ayam layer yang divaksinasi influenza kuadrivalen. Penelitian ini digunakan tiga ekor ayam layer ras Hy-line Brown yang divaksinasi vaksin influenza kuadrivalen yang berisi virus influenza tipe A dan B. Vaksinasi dilakukan sebanyak tiga kali dengan dosis 0,25 mL/ekor melalui rute intramuskular pada otot dada. Vaksinasi pertama dilakukan pada saat usia ayam 24 minggu, kemudian booster dilakukan pada usia 28 dan 32 minggu. Serum dan kuning telur dikoleksi dan diperiksa keberadaan antibodi spesifik-nya sejak minggu pertama sampai dengan minggu ke-20 pasca vaksinasi pertama. Keberadaan antibodi spesifik ditentukan dengan metode Agar Gel Precipitation Test (AGPT). Antibodi spesifik influenza telah terdeteksi pada serum dan telur sejak minggu ke-1 sampai dengan minggu ke-20 pasca vaksinasi pertama. Titer antibodi influenza tertinggi pada serum dan kuning telur mencapai 23, diperoleh setelah booster kedua. Hasil penelitian ini menunjukkan bahwa antibodi spesifik virus influenza tipe A dan B dapat di hasilkan pada serum dan kuning telur ayam., Influenza virus is able to infect respiratory tract, has a broad host range such as human and other species. Influenza type A an B is the most influenza type that infect human. Its transmission between humans occurred through droplets when coughing, sneezing and talking. This research aims to study the specific antibody response of influenza virus amongst layer chickens vaccinated with quadrivalent influenza. Three Hy-line Brown layers were vaccinated with quadrivalent influenza type A and B inactivated vaccines. Vaccination was carried out three times at a dose of 0.25 mL/chicken by intramuscular route. The first vaccination was carried out at 24 weeks of age, then boosters were performed at 28 and 32 weeks of age. Serum and egg yolk were collected and checked for the presence of specific antibodies from the first week to the 20th week after the first vaccination. The presence of specific antibodies and antibody titer was determined by the Agar Gel Precipitation Test (AGPT) method. Influenza-specific antibodies have been detected in serum and egg yolk from week 1 to week 20 after the first vaccination. The highest influenza antibody titer in serum and egg yolk reached 23, obtained at 9 and 10 weeks after the first vaccination. This highest titer is obtained after the 2nd booster. The results of this study indicate that specific antibodies to influenza virus types A and B can be produced in serum and chicken egg yolk.
Keyword: agar gel precipitation test (AGPT),, antibody, influenza, layer chicken

Judul: Evaluation of Influenza Antibody Titers in Serum and Egg Yolk of Layers Vaccinated with Influenza Vaccines.
Abstrak: Influenza belongs to the Orthomyxoviridae family which causes upper and lower respiratory tract infection. The aim of this study was to evaluate influenza-A specific antibody titers from serum and egg yolk of hens vaccinated with quadrivalent influenza vaccines. Three Hy-line Brown layers were vaccinated with quadrivalent influenza type A and B inactivated vaccines. Vaccination was carried out three times at a dose of 0.25 ml/chicken via the intramuscular route. The first vaccination was carried out at 24 weeks of age, then boosters were performed at 28 and 32 weeks of age. Serum and egg yolk were collected every week for 20 weeks since the first vaccination. Influenza A H3N2 and H1N1 antibody titers were determined using hemagglutination-inhibition (HI) test and agar-gel-precipitation (AGP) test. Influenza-A H3N2 and H1N1 specific antibodies were detected in both serum and egg yolk from the first to twenty weeks after the initial vaccination. Highest HI geometric mean titer (GMT) of H1N1 antibody reached 26.67 HIU at nine weeks post first vaccination (p.f.v), while H3N2 antibody reached 26 HIU at six weeks p.f.v. Our result showed that influenza antibodies can be produced in layers using quadrivalent vaccines and layer chickens are able to produce equivalent antibody levels for each influenza subtype.
Keyword: antibody, egg yolk, influenza, layer chickens, serum

Judul: Pengelolaan Ekosistem Magrove di Kecamatan Percut Sei Tuan Kabupaten Deli Serdang Sumatera Utara
Abstrak: Distric of Percut Sei TUan as one of coastal region in North Sumatera with mangrove resource which supporting the life of the society near the ecosystem, now faced to problems such as over exploitation and area conversion for various activity that causing damage until 79,8%. research about Mangrove Ecosystem Management on District of Percut Sei Tuan aim to know the potential and existing condition of mangrove ecosystem and also give directive policy strategic needed in managing mangrove ecosystem in this area.
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