Judul: Potensi gen bmpr-1b dan bmp-15 sebagai penciri untuk seleksi sifat prolifik pada domba ekor gemuk (DEG) Lombok
Abstrak: The Lomboknese fat tailed sheep (LFTS) is a local sheep that have been long time raised and well adapted to the extreme environments of Lombok island. The aim of this study was to identify the BMPR-1B and BMP-15 genes and their influences on the prolification of the LFTS. Polymorphism of BMPR-IB and BMP-15 genes were identified with restriction enzymes AvaII and HinfI. Data were analyzed based on allele frequencies and t test was applied to compare the mean values of litter size in different genotypes. The results of study showed that polymorphism of BMPR-1B gene resulted in three genotypes, namely : ++ (140 bp/140 bp), B+ (140 bp/109 bp/31 bp) and BB (109 bp/31 bp) with allele + and B frequencies were 0.839 and 0.161, respectively. While BMP-15 gene resulted in two genotypes, namely ++ (110 bp/110 bp) and G+ (141 bp / 110 bp) with allele G and + frequencies were 0.946 and 0.054, respectively. Chi-square test for confirming the Hardy-Weinberg equilibrium showed that both BMPR-1B and BMP-15 genes were in the equilibrium conditions. A highly significant difference (P<0.01) in the average of litter size in different genotypes was observed. The highest average of litter size was found in the genotype combination of the two genes (B+/G+), namely 1.781±0.123 lambs, while the lowest average was obtained in the genotype ++/++, namely 1.015 ± 0.063 lambs.
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Judul: Mutation Identification in Bone Morphogenetic Protein Receptor IB (BMPR-IB) and Bone Morphogenetic Protein 15 (BMP15) Genes are Associated with Proliferation in Prolific Garut Sheep.
Abstrak: Garut sheep is prolific sheep breed. A group of growth factor that is especially expressed in oocyte and ovarian follicle is grouped as oocyted-derived growth factor (ODGF), also known as the Tansforming Growth Factor β (TGFβ). Proliferation in sheep controlled by three main factor of TGFβ that are Bone Morphogenetic Protein Receptor IB (BMPR-IB), Bone Morphogenetic Protein 15 (BMP15) and Growth Differentiation Factor 9 (GDF9). Mutation identification in the 6th exon of BMPR-IB gene and 2nd exon of BMP15 gene was done in this research. Genotype composition of BMPR-IB gene in prolific Garut sheep consist of BB (11%), B- (69%), and – (20%). The frequency of B allele (mutant type) was 46% and -- allele (wild type) was 54%. Genotype composition of BMP15 gene was ++ (100%). These implied that there was no Galway mutation in BMP15 gene. Further detection of mutation on the 2nd exon of BMP15 gene using SSCP method was found four type of mutation denoted by A, B, C, D. All genotype had successfully detected; either as single or combined gene; were suggested that there was no correlation between gene and litter size.
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Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4