Judul: Optimasi Penggunaan IgY Dan IgG Sebagai Antibodi Penangkap Dan Pendeteksi Antigen Ekskretori Sekretori (ES) Fasciola gigantica Dalam Uji Elisa
Abstrak: Fasciolosis is a parasitic disease of domestic ruminant that cause high economic loss. Currently diagnosis of fasciolosis relies on microscopic examination of Fasciola sp. eggs in fecal sample. Serological test such as ELISA has been developed to improve the diagnostic method of fasciolosis. This study was designed to optimize the use of polyclonal antibodies (IgY and IgG) as capture antibody and detection antibody in ELISA assay to detect excretory secretory (ES) of Fasciola gigantica. The conformation was done in two models which compared IgY (9,57 mg/ml) and IgG (3,75 mg/ml) as capture antibody. The first conformation was IgY as capture antibody in 1:1000 dilution and IgG as detection antibody in 1:100, 1:1000, and 1:10000 dilutions. The second conformation was IgG as capture antibody in 1:1000 dilution and IgY as detection antibody in 1:100, 1:1000, and 1:10000 dilutions. The results showed that IgY as capture antibody in 1:1000 dilution and IgG as detection antibody in 1:100, 1:1000, 1:10000 dilutions were able to capture ES antigen 3,87; 2,85; and 1,65 times than cut off value. The other conformation with IgG as capture antibody in 1:1000 dilution was able to capture ES antigen as much as 1,91 times than cut off value with IgY as detection antibody in 1:100 dilution. This showed that the good optimation ELISA was IgY as capture antibody in 1:1000 dilution and IgG as detection antibody in 1:100 dilution.
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Judul: Pengembangan metode elisa untuk mendeteksi keberadaan koproantigen fasciola gigantica pada ruminansia: model uji diagnostik untuk human fasciolosis
Abstrak: The study was designed to develop ELISA method to detect coproantigen of Fasciola gigantica in ruminant as a model for human fasciolosis diagnostic test. Fasciolosis Diagnostic Test. Under direction of FADJAR SATRIJA and SRI MURTINI. Excretory Secretory (ES) antigen were isolated from adult stage of F. gigantica incubated in the RPMI medium at 370C for 4 hours. Each worm produced roughly 0.18 mg ES antigen. ES protein showed five protein bands with the molecular weight of 104.99, 90.05, 56.43, 47, 30.27 kDa in SDS-PAGE analysis. P olyclonal antibodies against ES antigen were raised from rabbits and chicken egg yolk. These polyclonal antibodies were used in sandwich ELISA for detection of coproantigen using IgY as capture antibody and IgG as detection antibody. Specificity and sensitivity test revealed that the ELISA have 87,5% sensitivity and 100% specificity compared to gold standard post-mortem examination.
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Judul: Budaya Perkawinan Anak dan Kerentanan Rumah Tangga yang Ditimbulkan pada Masyarakat Madura
Abstrak: Kasus perkawinan anak masih banyak ditemukan di Indonesia, salah satunya yaitu di Madura. Perkawinan anak di Madura merupakan suatu budaya yang dianggap sah meskipun bertentangan dengan syarat Undang-Undang Perkawinan dan Undang-Undang Perlindungan Anak. Penelitian ini bertujuan untuk menganalisis budaya perkawinan anak dan kerentanan rumah tangga. Metode yang digunakan dalam penulisan adalah pendekatan kuantitatif yang didukung oleh data kualitatif dengan teknik pemilihan responden secara purposive. Unit analisis penelitian ini adalah rumah tangga. Hasil penelitian menunjukkan perkawinan anak memiliki kerentanan yang lebih besar dari pada perkawinan non anak. Di antara pasangan perkawinan anak, pada kerentanan ekonomi jenis pasangan yang paling rentan adalah anak perempuan dengan laki-laki dewasa, pada kerentanan sosial dan psikologi jenis pasangan yang paling rentan adalah anak perempuan dengan anak laki-laki.
Keyword: perkawinan anak, karakteristik, kerentanan