Judul: Kondisi ekosistem mangrove berdasarkan indeks kualitas lingkungan, morfometrik daun, dan kepadatan makrozoobentos di kabupaten Sampang-Madura, Jawa Timur
Abstrak: Ekosistem mangrove dapat tumbuh dan bertahan hidup dalam kondisi lingkungan yang ekstrim. Tekanan lingkungan yang kurang sesuai untuk mengrove memberikan respon yang berbeda. Kerusakan dan hilangnya ekosistem mangrove dapat menyebabkan dampak negatif jangka panjang terhadap kehidupan manusia di sekitarnya.
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Judul: Kondisi ekosistem mangrove berdasarkan indikator kualitas lingkungan dan pengukuran morfometrik daun di muara sungai Bengawan Solo, Ujung Pangkah, Gresik, Jawa Timur
Abstrak: Pertumbuhan penduduk yang tinggi dan pesatnya pembangunan di wilayah pesisir menyebabkan tekanan ekologis terhadap ekosistem pesisir, khususnya ekosistem mangrove. Hal ini berdampak terhadap kerusakan ekosistem hutan mangrove baik secara langsung maupun tidak langsung. Demikian juga yang terjadi pada hutan mangrove di Jawa Timur, yang telah mengalami kerusakan baik akibat aktivitas manusia maupun secara alami. Penelitian ini bertujuan untuk menganalisis kualitas lingkungan mangrove di Muara Sungai Bengawan Solo, Ujung Pangkah, Gresik, Jawa Timur berdasarkan indikator kualitas lingkungan mangrove dan mengetahui kondisi ekosistem mangrove berdasarkan sebaran morfometrik daun mangrove.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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