Judul: Analisis Keragaman Genetik Tanaman Karet Hasil Persilangan antara RRIM 600 dan PN 1546 dengan Menggunakan Teknik RAPD
Abstrak: Low productivity of rubber product had been the main problem in public rubber plantation in Indonesia. New assembly of excellent genotipe rubber plant requires 25-30 years. The more appropriate and accurate method is needed to early determine the new rubber plant clone. This research aimed to shows genetic variation of rubber plants-crossed product between RRIM 600 and PN 1546 with RAPD technique. Two DNA samples from rubber plants parent and 25 DNA samples from rubber plants-crossed products were isolated and amplified by using 16 mTcCIR combination primers and 60 RAPD combination primers. Amplification by using 16 mTcCIR combination primers produced a specific selected marker candidates, while amplification by using 60 RAPD primers produced polymorphism ribbon pattern in agarose gel. Phylogenetic tree was created based on the ribbon to identify the group and family distances of rubber plants-crossed product. Result showed that combination primer ‟d‟ and ‟i‟can be developed into specific marker candidates for RRIM 600. Phylogenetic tree showed that 25 rubber plants-crossed product have 10 high family groups with coeficient 89%. The family group showed that 25 rubber plant-crossed product had high diversity.
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Judul: Analisis Sebaran Genetik Hasil Persilangan Tanaman Karet RRIM 600 dan PN 1546 Menggunakan Teknik RAPD
Abstrak: The main problem in providing recommended rubber clones for rejuvenating process is breeding cycle length that took 25-30 years. Therefore, before rejuvenated an early detection and selection for superior clones is needed. RAPD (Random Amplified Polymorphic DNA) molecular marker is one of alternative method that can be used for the selection. This research focusing on genetic diversity observation of cross hybrid rubber plant between RRIM 600 and PN 1546. This research used 56 primers that consist of 16 combination primers (named with alphabet from letter A to letter P) and 40 RAPD primers (named OPH-OPJ (1-20)). Version 2.02 of NTSys program was used as tabulation method. DNA amplification of 16 combination primer produced 5 primary candidates for molecular marker, including primer C, primer D, primer N, primer M, and primer I. Each primer has their own specification. Primer D used for RRIM 600 only. Both primer C and M used for PN 1564 only. Primer N and I used for intermediate only. DNA amplification result of 40 RAPD primers has coefficient value range between 0.89 until 0.98. At 0.89 coefficient, 27 rubber plants separated in two major groups. Dendogram analysis also showed that the elders were distantly related.
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Judul: Karakteristik Anatomi Skelet Kepala Buaya senyulong (Tomistoma schlegelii).
Abstrak: Buaya sepit atau senyulong (Tomistoma schlegelii) merupakan jenis buaya asli Indonesia yang berhabitat di sungai-sungai Sumatera, Kalimantan, dan juga pedalaman Sulawesi. Salah satu ciri khas buaya senyulong adalah bentuk moncong yang runcing dan sempit, namun hingga saat ini informasi mengenai struktur skelet kepala buaya senyulong sangat terbatas. Penelitian ini bertujuan untuk mempelajari karakteristik anatomi skelet kepala buaya senyulong yang dikatikan dengan fungsi dan perilakunya. Penelitian dilakukan secara eksploratif dengan melakukan pengamatan, pencatatan, dan pengambilan gambar dari skelet kepala buaya senyulong. Skelet kepala berukuran panjang 70 cm, lebar 29 cm, dan tingggi 17 cm dengan permukaan kasar pada os nasale, os frontale, os maxilla, os mandibula, os lacrimale, os jugale. Jumlah gigi pada buaya ini sebanyak 78 buah, terdiri dari 4 buah gigi premaxila, 16 buah gigi maxila, dan 19 buah gigi mandibula. Pada regio orbitalis ditemukan dua pasang fenestrae temporales yang berkembang baik. Fenestra supratemporale merupakan lubang oval yang membentang di sepanjang os parietale. Fenestra infratemporale berbentuk segitiga dan dibatasi oleh os squamosum, os postorbitale, os jugulare, dan os quadratum. Os mandibula hewan ini mempunyai symphysis mandibularis yang panjang yaitu garis yang melintang di corpus mandibulae. Secara fungsional ukuran symphysis mandibularis berkaitan dengan adaptasi hewan terhadap diet makan.
Keyword: kepala, moncong, Tomistoma schlegelii