Judul: Studi Morfologi dan Molekuler Boesenbergia angustifolia (Hallier f.) Schltr. dan Boesenbergia sp.
Abstrak: Zingiberaceae is the largest family of Zingiberales with 53 genera, over 1200 species and spread in the tropics. Boesenbergia is the one of Zingiberaceae genera, that has not been explored, and therefore, in this research morphological and phylogenetic studies were conducted using Boesenbergia angustifolia and Boesenbergia sp. of Sumatra. The method used in this study is the observation of morphological characters and molecular characters in based of variation in Internal Transcribed Spacer (ITS) sequence are used for phylogenetic tree construction. DNA sample was isolated using cetyltrimethylammonium bromide based method of Doyle & Doyle modification. Amplification of ITS region resulted in ± 800 bp amplicon. Sequencing DNA of ITS region resulted in ± 788 bp amplicon from Boesenbergia angustifolia and ± 789 bp amplicon from Boesenbergia sp. Recontruction of phylogenetic tree was analysed using the Phylogenetic Analysis Using Parsimony program. The morphological characters of Boesenbergia angustifolia and Boesenbergia sp have similar flowers and inflorescence character. Suggestion that they are of the same genera. This is also supported by ITS DNA sequence, both of these plant species have 17 bases difference. Phylogeneticly, these plant species also form branching close to the high level of confidence with 97% bootstrap value. They are of the same veriety.
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Judul: Analisis Biomolekuler Gen Internal Transcribed Spacer (ITS) dalam Studi Filogenetik Zingiber loerzingii Valeton (Zingiberaceae)
Abstrak: Zingiberaceae consists of 50 genera and 1400 species distributed in tropical and subtropical areas. The center of the diversity of Zingiberaceae is South Asia and Southeast Asia. Zingiber loerzingii is only found in Sumatera. Unlike other species, this species has not been well studied so the information is still limited, whereas the clarity of plant identity is very crucial. The present study was aimed to determine phylogenetic relationship of Zingiber loerzingii and its relative based on the Internal Transcribed Spacer (ITS) gene. DNA sample was isolated using cetyltrimethylammonium bromide based method of Doyle & Doyle modification. Amplification of ITS region resulted in ± 700 bp amplicon. Sequencing of ITS region of Zingiber loerzingii produced 813 bp DNA sequences that has high rate of evolution. Recontruction of phylogenetic tree was analysed using the Phylogenetic Analysis Using Parsimony program. The results of phylogenetic analysis showed that the genus of Zingiber formed a clade with bootstrap value of 61.9%. The clade consisted of two clades, clade I with bootstrap 62% and clade II with bootstrap 70.8%. Therefore, we conclude that the genus Zingiber is possibly monophyletic. Zingiber loerzingii is nested in clade I with the nearest or sister taxa: Zingiber rubens, Zingiber orbiculatum dan Zingiber fragile.
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Judul: Feeder kelinci ternak otomatis menggunkan prinsip aktuator solenoid
Abstrak: Feeder kelinci ternak otomatis merupakan prototipe yang diciptakan dengan pengaturan distribusi pakan berbentuk pellet pada konveyor sekrup termodifikasi dengan tambahan pintu akses teraktivasi oleh aktuator solenoid. Mikrokontroler Arduino Uno R3 pada prototipe ini digunakan untuk proses perhitungan jarak pintu akses, waktu tempuh pakan, dan kecepatan distribusi pakan. Prototipe ini bermanfaat untuk para pembudidaya/peternak kelinci sebagai inovasi teknologi dalam kegiatan pemeliharaan kelinci secara moderen. Pakan kelinci diatur melalui rotasi motor stepper pada penakar pakan sehingga dapat mengukur porsi pakan secara akurat. Penggunaan motor DC dilengkapi gearbox bertujuan mengendalikan konveyor sekrup agar berputar dengan konstan sehingga menghasilkan gaya dorong pada pakan yang akan didistribusikan. Hasil perhitungan kecepatan distribusi pakan menentukan waktu aktivasi aktuator solenoid yang akan membuka pintu akses pakan untuk keluar dari konveyor sekrup.
Keyword: Aktuator solenoid, feeder, penakar pakan, konveyor sekrup