Judul: Integression of opaque-2 gene into downy mildew resistance lines, utilizing Marker Asisted Selection (MAS) in developing hybrid maize
Abstrak: Jagung bermutu protein tinggi (QPM= Quality Protein Maize) merupakan salah satu sumber protein nabati yang diperlukan oleh manusia dan ternak monogastric karena mengandung gen mutan opaque-2 yang mengekspresikan peningkatan lisin dan triptofan pada endosperma menjadi lebih tinggi dibandingkan dengan jagung normal. Kendala pengembangan QPM di Indonesia adalah semua koleksi QPM yang ada rentan penyakit bulai. Pemanfaatan marka molekuler sebagai alat bantu seleksi (MAS = Marker Assisted Selection) untuk mengintrogresikan gen mutan opaque-2 ke galur elit resisten terhadap Peronosclerospora maydis dapat mempercepat pembentukan populasi atau hibrida QPM komersial yang resisten terhadap penyakit tersebut. Tujuan akhir yang ingin dicapai pada penelitian ini adalah mendapatkan kandidat varietas jagung hibrida QPM, resisten terhadap penyaki bulai dan hasil tinggi. Penelitian ini dilaksanakan dalam empat bagian percobaan. Penelitian pertama: Pendugaan ragam dan model genetik karakter ketahanan terhadap P. maydis. Dibuat masing-masing 7 macam populasi (P1, P2, F1, F2, BC1P1, BC1P2 dan F3) dari set persilangan CML161 x MR10 dan CML161 x Nei9008 kemudian diinokulasi dengan konidia P. maydis secara semi buatan, menggunakan rancangan acak kelompok (RAK). Hasil percobaan menunjukkan bahwa ketahanan penyakit bulai dikendalikan oleh gen-gen yang bersifat kuantitatif dan tingkat ketahanannya secara nyata diperankan oleh aksi gen-gen aditif, dominan dengan pengaruh interaksi epistasis komplementer pada set persilangan MR10 x CML161 dan interaksi epistasis duplikat pada set persilangan Nei9008 x CML161.
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Judul: Modifikasi Pati Sagu dengan Teknik Heat Moisture Treatment (HMT) dan Aplikasinya dalam Memperbaiki Kualitas Bihun
Abstrak: Maize is a strategy commodity in the development of Indonesian agriculture and economic since it’s multifunction as food and feed. However, maize crop cultivated by farmer at the present time is lack for the low protein quality. Therefore a breeding program for improving protein quality is essential to be conducted, especially for lysine and tryptophan. The objectives of this research are to identify and select QPM lines which are tolerant to downy mildew (Peronoscelrospora maydis) and soil acidity. Genetic material used was derived from a cross breeding between QPM lines (SK-1_QPM) and lines which is resistant to downy mildew and tolerance to acidic soil (SMRG). The research was conducted in four stages. The first stage was development of F2 population and identification of opaque-2 gene in F1 population using SSR specific marker phi057, conducted in ICERI Molecular laboratory from February until March 2008. The second stage was screening of F2 population selected using SSR marker to downy mildew, conducted at Maros Experimental Station from June until August 2008 and arranged in Augmented Design. The third stage was the screening of F2 population selected using SSR marker to alumunium toxicity in nutrient solution containing 10 ppm Al3+ and haematoxylin, conducted at ICERI Plant Breeding Laboratory from July until September 2008 and was arranged in Randomized Complete Design with two replications. The fourth stage was evaluation of F2 population on the acidic soil, conducted in Barambai Experimental Station, South Kalimantan from July until November 2008 and was arranged in Randomized Complete Block Design with two replications. The research result showed that there were 210 F2 plants carrying opaque-2 gene from the F2 population analysed, subsequently they were evaluated separately to downy mildew (Peronosclerospora maydis), alumunium toxicity (in the nutrient solution), and soil acidity. The evaluation for resistance to downy mildew revealed 19 plants were very resistant, 53 resistant plants, 62 moderately-resistant plants, and 76 susceptible plants. Evaluation at seedling stage to alumunium toxicity resulted 3 very tolerant plants, 5 tolerant plants, 12 moderately- tolerant plants, and 24 susceptible plants. None of the plants being analysed showed resistance to downy mildew and also tolerance to aluminium toxicity. Furthermore, we found one resistant to downy mildew and tolerant to aluminium toxicity, 5 plant moderately resistant and moderately tolerant, and 5 plant suceptible to both stresses. Evaluation of F2 population tolerance to soil acidity indicated that in general, maize character showed different responses. The principle component analysis (PCA) was clustering the tolerance genotypes to soil acidity into four clusters, namely 60 very tolerant plants, 55 tolerant plants, 47 moderately- tolerant plants, and 59 susceptible plant. There were 4 plants that very resistant to downy mildew, very tolerant to soil acidity and also have higher yield than Bima-2, Bima-3 and SMRG. Moreover, there were 5 tolerant plants, 7 moderately tolerant plants, and 27 susceptible plants to downy mildew and soil acidity.
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Judul: Kebijakan pemerintahan daerah kabupaten Situbondo dalam kerangka otonomi daerah di bidang perikanan tangkap
Abstrak: Undang-Undang nomor 31 tahun 2004 tentang Perikanan menyatakan bahwa pengelolaan perikanan merupakan suatu hal yang harus dilaksanakan baik oleh pemerintah pusat maupun daerah.
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