Judul: Coastline Change Using Landsat Satellite Imagery on the North Coast of Java, Banten
Abstrak: Perubahan garis pantai yang disebabkan abrasi dan akresi akan menunjukkan kecenderungan perubahan garis pantai yang terkikis atau bertambah. Deteksi perubahan garis pantai menggunakan teknologi penginderaan jauh merupakan salah satu metode observasi yang dapat dilakukan secara multi temporal. Tujuan penelitian untuk mengetahui perubahan garis pantai dengan menggunakan citra satelit di Pantai Utara Jawa, Banten dengan metode Digital Shoreline Analysis System (DSAS). Proses analisis data meliputi empat tahapan, yaitu pengolahan citra awal, ekstraksi garis pantai, perhitungan perubahan garis pantai, dan pengolahan data pendukung. Kecepatan angin dominan berasal dari arah barat yang berkisar antara 3,60-5,70 m/s. Pergerakan arus di daerah kajian menunjukkan pergerakan arus bergerak dari timur menuju barat. Nilai tinggi gelombang mencapai 0,21 m dengan periode gelombang 0,01-3,09 m/s. Hasil perhitungan nilai bilangan formzhal menghasilkan nilai tipe pasang surut sebesar 4,05 yang termasuk ke dalam tipe pasang surut tunggal. Abrasi terbesar terjadi di Kecamatan Mauk dengan kemunduran garis pantai sebesar 928,55 m, sedangkan akresi terbesar terjadi di Kecamatan Kemiri dengan penambahan garis pantai sebesar 380,99 m. Kata Kunci : Abrasi, Akresi, DSAS, Ekstraksi, Penginderaan Jauh
Keyword: Abrasion, Accretion, DSAS, Extraction, Remote Sensing

Judul: Detection of Coastline Changes Using Landsat Imagery in the Coastal of Sumur, Pandeglang, Banten.
Abstrak: Pesisir merupakan kawasan peralihan antara darat dan laut sehingga sangat potensial sebagai lokasi pembangunan dan cenderung mendapat tekanan yang berat sehingga rawan terhadap perubahan. Penginderaan jauh efisien dan efektif dalam pemantauan perubahan garis pantai. Penelitian ini dilakukan di pesisir Sumur, Pandeglang, Banten. Metode Digital Shoreline Analysis System (DSAS) digunakan untuk menghitung statistik laju perubahan garis pantai. Program DSAS membutuhkan beberapa parameter dalam pengukuran laju perubahan garis pantai seperti baseline, shoreline yang sudah terkoreksi pasang surut, dan transek. Perubahan garis pantai dihitung pada transek dengan perhitungan Net Shoreline Movement (NSM) dan End Point Rate (EPR). Abrasi terjadi pada subzona A2, A3, B3, C3, D1, D2, dan D3.Wilayah yang mengalami akresi meliputi subzona A1, A2, A3, B1, B2, dan B3. Perubahan garis pantai berupa abrasi terbesar terjadi di sub zona D1 dengan nilai -77 m. Nilai perubahan berupa akresi terbesar terjadi pada sub zona A3 yaitu sebesar 61,11 m. Perubahan yang terjadi disebabkan adanya arus menyusur pantai yang dipengaruhi oleh angin. Perubahan garis pantai yang terjadi bersifat ringan hingga sedang., Coastal area is a transitional area between land and sea which is very potential to be developed and tends to be under heavy pressure, thus it is vulnerable to change. Remote sensing is an efficient and effective tools in monitoring shoreline changes. This research was conducted on the coast of Sumur, Pandeglang, Banten. The Digital Shoreline Analysis System (DSAS) method was used to calculate the rate of change of the time series of shoreline vector data. The DSAS program requires several parameters in measuring the rate of shoreline change in this study, such as the baseline, tide-corrected shoreline, and transects. Shoreline changes were calculated on the transect by calculating the Net Shoreline Movement (NSM) and End Point Rate (EPR). Abrasion was occurred in subzones A2, A3, B3, C3, D1, D2, and D3. Accretion was occurred in subzones A1, A2, A3, B1, B2, and B3. Larges abrasion in shoreline was occurred in sub zone D1 with a value of -77 m. The larges accretion occurred in the A3 sub zone, which was 61.11 m. The changes are caused by the current along the coast which is influenced by the wind. Changes in the coastline that occur are mild to moderate.
Keyword: Abrasi, Akresi, DSAS, Garis Pantai, Landsat

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
Keyword: