Judul: Inhibition of carmoisine and rhodamine toxic effects on rat’s spleen lymphocytes proliferation activity by Urtica dioica L. leaf extract
Abstrak: Urtica dioica L. has long been known as a herbal remedy and nutritious addition to the diet. It has been consumed for a long time as medicinal plants in many parts of the world. This study aimed to observe the effect of U. dioica L. leaf extracts in enhancing proliferation of rat’s spleen lymphocytes. Furthermore, this study also aimed to observe the capability of U. dioica L. leaf extracts in defending lymphocyte against toxic effects of carmoisine and rhodamine B . 30 Sprague Dawley rats were split into three groups, which are control, 0.1 g/kg BW/day dose, 1 g/kg BW/day dose group. The control group was given drinking water while the others were given U. dioica L. leaf crude extracts at their dose respectively. After 90 days of treatment, lymphocyte cells from the rat’s spleen then isolated and tested using MTT proliferation assay with addition of mitogen, and various concentration of carmoisine and rhodamine B. Stimulation index of spontaneous proliferation in 0.1 g/kg BW/day dose group was higher than the other groups. Mitogen-induced proliferation shows an increased proliferation activity in control and 1 g/kg BW/day dose group, while in 0.1 g/kg BW/day dose group a decreased proliferation activity was observed. U. dioica L. leaf crude extracts given at 1 g/kg BW/day shows that the rat’s lymphocyte proliferation activity at the presence of carmoisine was higher than control and 0.1 g/kg BW/day dose group. Likewise, at the presence of rhodamine B, the rat’s lymphocyte proliferation activity of 1 g/kg BW/day dose group was higher than control and 0.1 g/kg BW/day dose group. It is concluded that U. dioica L. leaf crude extract given at 1 g/kg BW/day could inhibit carmoisine and rhodamine B toxic effect on rat’s spleen lymphocytes proliferation activity.
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Judul: Pengaruh Ekstrak Biji Bligo (Benincasa hispida Thunb Cogn) terhadap Penghambatan Efek Toksik Tartrazin dan Rhodamin pada Aktivitas Proliferasi Limfosit Tikus
Abstrak: Bligo (Benincasa hispida Thunb Cogn) is one of medical plants that would be useful as medicine for some diseases. Previous study of bligo showed that bligo extract has the ability to cure ulcer in rats and should no toxicity in rat serum analysis. This effect is estimated to be due to some bioactive compounds, like fenolic, terpene, flavonoid c-glycoside and sterols that have antioxidant effect. This bioactive compound were asumed to be able to increase lymphocyte cell proliferation activity. This research purpose is to get the scientific data and to know the effect of consuming bligo seed extract in rat growth and on spleen lymphocyte cell proliferation activity. Three groups of rats, including control or 0 g/kg bw of bligo seed extract, 0.1 g/kg bw bligo seed extract, and 1 g/kg bw bligo seed extract. After 90 day treatment, splenocytes of rats were cultured in the presence of LPS 12.5 μg/ml culture, tartrazine 90 μg/ml, 180 μg/ml, 270 μg/ml culture, or rhodamine 6 μg/ml, 12 μg/ml, 18 μg/ml culture. After 72 hours of incubation, spleen lymphocyte cell proliferation activity was measured by MTT method. Results of this research showed that 0.1 g/kg bw bligo seed extract has increased proliferation activity by 14.30% on treatment with LPS, -0.09% on tartrazine 90 μg/ml culture, 11.42% on tartrazine 180 μg/ml culture, 21.92% on tartrazine 270 μg/ml culture, meanwhile, lymphocyte cultured with rhodamine 6 μg/ml culture, rhodamin 12 μg/ml culture, and rhodamin 18 μg/ml culture, has increased proliferation activity of 25.22%, 7.42%, and 12.19% respectively, compare to that of lymphocyte cultured with media alone. And 1 g/kg bw bligo seed extract can increase proliferation activity by 12.30% on LPS, 24.38% on tartrazine 90 μg/ml, 11.54% on tartrazine 180 μg/ml, 52.74% on tartrazine 270 μg/ml, -4.03% on rhodamine 6 μg/ml, -15.04% on rhodamin 12 μg/ml, and -17.66% on rhodamin 18 μg/ml, compare to that of control culture. Bligo seed extract can prevent toxic effect of tartrazine and rhodamine. The effective result was from rats that gavaged with 0.1 g/kg bw bligo seed extract. The conclusion of this research is bligo is good for health.
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Judul: Analisis Dinamik Struktur Gedung Terhadap Beban Gempa (Studi Kasus Tower Altiz – Tangerang Selatan).
Abstrak: Penelitian ini bertujuan untuk melakukan pemodelan gedung, menganalisis besarnya periode getar dan kekakuan yang dimiliki gedung, menentukan mode shape dan partisipasi massa, serta menganalisis besarnya gaya geser dasar gempa. Objek penelitian adalah Tower Altiz. Prosedur penelitian mengacu kepada SNI 1727-2013, SNI 1726-2012, SNI 2847-2013, dan PPURG 1987. Berdasarkan hasil penelitian, pemodelan Tower Altiz telah berhasil dilakukan. Periode getar yang dialami Tower Altiz adalah 2.735 detik. Mode shape dari Tower Altiz telah berhasil didapatkan. Untuk besarnya partisipasi massa pada arah x yang lebih dari 90 %, didapat pada mode ke-10, sedangkan untuk partisipasi massa pada arah y didapat pada mode ke-11. Gaya geser dasar statik (Vstatik) sebesar 11359.164 kN sedangkan besarnya gaya geser dasar dinamik (Vdinamik) 8339.260 kN untuk arah gempa-x dan 7722.238 kN untuk arah gempa-y. Faktor skala desain untuk gaya geser dinamik (Vdinamik) sumbu-x adalah 1.15, sedangkan untuk sumbu-y adalah 1.25. Besarnya gaya geser dinamik (Vdinamik) yang diperoleh untuk sumbu-x dan sumbu-y adalah 9655.289 kN.
Keyword: gaya geser, gedung, gempa, struktur