Judul: Pengetahuan, Persepsi, Dan Sikap Ibu Terhadap Paud Nonformal
Abstrak: Lembaga pendidikan usia dini diselenggarakan sebagai sarana alternatif dalam memaksimalkan perkembangan anak pada rentang usia dini. Lembaga PAUD nonformal diselenggarakan pemerintah dengan tujuan pemerataan akses pendidikan untuk masyarakat berpenghasilan rendah. Penelitian ini bertujuan menganalisis hubungan persepsi, pengetahuan, dan sikap ibu terhadap lembaga PAUD nonformal. Penelitian ini menggunakan 100 ibu siswa PAUD nonformal sebagai responden. Pengambilan contoh dalam penelitian menggunakan metode random sampling. Hasil penelitian menunjukkan bahwa ibu sudah memiliki persepsi baik terhadap PAUD nonformal dan berpandangan bahwa biaya pendidikan di PAUD nonformal lebih terjangkau. Pengetahuan ibu berada pada kategori sedang namun belum mengetahui usia minimal keikutsertaan anak pada lembaga PAUD. Sikap ibu berada dalam kategori netral terhadap lembaga PAUD. Hasil uji hubungan memperlihatkan bahwa usia, lama pendidikan, dan pendapatan keluarga berhubungan positif dengan persepsi. Pengetahuan memiliki hubungan positif dengan lama pendidikan. Persepsi memiliki hubungan positif dengan pengetahuan dan sikap.
Keyword: PAUD Nonformal, pengetahuan, persepsi, sikap

Judul: Hubungan Antara Karakteristik Dan Sikap Ibu Rumah Tangga Dengan Tingkat Partisipasi Dalam Program Rumah Pintar
Abstrak: Pendidikan merupakan hal yang penting bagi setiap manusia, sesuai pada Pembukaan UUD 1945 bahwa setiap masyarakat Indonesia berhak untuk memperoleh pendidikan. Namun belum semua masyarakat terutama daerah pedesaan dapat merasakan adanya akses pendidikan. Oleh sebab itu dibutuhkan program pendidikan non formal yang dapat memfasilitasi masyarakat desa untuk memperoleh pendidikan. Melalui Solidaritas Istri Kabinet Indonesia Bersatu (SIKIB) merancang sebuah pendidikan non formal yang dapat memberikan akses pendidikan kepada masyarakat pedesaan. Kehadiran rumah pintar diharapkan akan mampu meningkatkan kesejahteraan, perekonomian serta keterampilan masyarakat desa. Beberapa fasilitas yang disediakan dari adanya program terdiri dari lima sentra antara lain sentra buku, sentra bermain dan permainan, sentra komputer, sentra audio visual atau panggung dan sentra kriya, semua layanan mengarahkan pada keterlibatan kebutuhan masyarakat desa. Jenis penelitian ini adalah penelitian kuantitatif didukung oleh data kualitatif dengan instrumen kuesioner dan panduan wawancara mendalam. Hasil penelitian ini akan memperlihatkan bagaimana hubungan antara karakteristik dan sikap ibu rumah tangga dengan tingkat partisipasi dalam program rumah pintar.
Keyword: masyarakat desa, partisipasi, SIKIB, sentra

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable