Judul: Identifikasi Postlarva Famili Gobiidae dari Muara Sungai Kedurang, Bengkulu melalui DNA Barcode
Abstrak: Gobiidae family fish postlarvae is one of seasonal migrant biota in a very large number. It is difficult to identify fishes species according to morphological characteristics of postlarvae because most of the fish identification guide is based on adult characters. The aim of this study was to identify the species composition of Gobiidae postlarvae that migrated in Kedurang estuary, Bengkulu through DNA Barcode. A total of 22 tails Gobiidae family postlarvae which were preserved in alcohol used as sampels. Segment of CO1 gene was used for barcoding purpose. Amplified of CO1 gene were successfully yielded in 700-800 bp fragment. Sample no 4, 5, 6, 10, 11, 12, 14, 16, 18, 21, 22 and 22 were identified as Stiphodon atratus, sample no 19 and 20 were identified as Amblyeleotris sungami while the sample no 3 were identified as of Novem Genus of subfamily Sicydiinae. Phylogeny tree reconstruction using the Neighbor Joining (NJ) bootstrap 1000x showed two major clades. One clade contains Stiphodon atratus and Novem Genus, other clades include only Amblyeleotris sungami
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Judul: DNA Barcode as an Alternative of Mantis Shrimp Species Identification
Abstrak: Udang mantis atau yang dikenal dengan udang ronggeng merupakan salah satu anggota Subfilum Crustacea Ordo Stomatopoda, Beberapa spesies udang mantis dikenal sebagai komoditi ekspor dan makanan eksotis. Beberapa udang mantis yang bernilai ekonomi tinggi adalah anggota famili Harpiosquillidae dan Squillidae. Udang-udang mantis tersebut biasa ditangkap dari Laut Jawa dan Laut Cina Selatan. Penelitian ini bertujuan untuk mengaplikasikan teknik DNA barcoding sebagai laternatif mengidentifikasi udang mantis sampai ke tingkat spesies. Sebanyak 34 sampel udang mantis berasal dari Jambi, Cirebon, dan Aceh yang digunakan adalah spesimen koleksi yang diawetkan dalam alkohol. Identifikasi spesies dilakukan berdasarkan morfologi. Ekstraksi DNA dilakukan dari otot tungkai dan ruas gen CO1 diamplifikasi sebagai ruas DNA yang dijadikan barcode. Amplifikasi gen CO1 berhasil dilakukan untuk semua sampel yang kesemuanya menghasilkan amplikon yang multiband. Perunutan nukelotida hanya berhasil dilakukan untuk tujuh sampel, yaitu Harpiosquilla harpax (Sampel No. 34 dan No. 37 asal Jambi), Harpiosquilla stephensoni (sampel No. 1 dan No. 2 asal Cirebon), dan Carinosquilla multicarinata (sampel No. 9 dan No. 11 asal Cirebon, dan No. 22 asal Aceh). Berdasarkan ruas gen CO1, C. multicarinata asal Aceh memiliki kekerabatan yang berbeda dengan C. multicarinata asal Cirebon.
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Judul: Natrium lignosulfonat sebagai agen pendispersi pada mortar
Abstrak: Lignosulphonate, a modification product of lignin waste from pulp and paper industry can be used as water reducer in mortar mixture. This substance can influence the mechanical characteristic of mortar such as diameter of flow, flexural strength, compressive strength, and setting time of cement paste. Lignosulphonate shows it function as dispersant on cement hydration at 3 days that analyzed with x-ray difractometer. Research result show that flow of fresh mortar rise with increase concentration (from weight of cement) of synthetic sodium lignosulphonate (NaLS). It show that mortar homogenity also increasing. The result of flexural strength and compressive strength test use synthetic NaLS 0.1-0.3% from weight of cement show that addition synthetic NaLS 0.2% is the best result because it has the biggest of flexural strenght and compressive strength value. Initial setting time of cement paste increase, about 50% with addition of synthetic NaLS 0.2%. Whereas the final setting time decrease, about 8%. Cement hydration test show increase hydration product peak, Ca(OH)2 dan calcium silicate hydrate (C-S-H).
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