Judul: Analisis Biaya Produksi Kursi dan Lemari: Studi Kasus di PT. Mustika Jepara Kota Jakarta Timur, DKI Jakarta
Abstrak: Jati (Tectona grandis Linn.f.) termasuk famili Verbenaceae, memiliki berat jenis 0,67 dan kelas kuat II. Jati di Jawa tumbuh di daerahdaerah dengan musim kemarau yang sangat panjang pada tanah yang agak kering berkala atau sangat kering, sampai ketinggian kurang lebih 650 m dari permukaan laut. Meskipun keras dan kuat, kayu jati mudah dipotong dan dikerjakan, sehingga disukai untuk membuat furniture dan ukir-ukiran. Penelitian ini bertujuan untuk menganalisis biaya produksi, Break Event Point (BEP), profabilitas yang ditunjukan oleh presentase ROI, dan harga pokok kursi dan lemari di PT. Mustika Jepara. Hasil penelitian menunjukkan bahwa biaya produksi kursi tamu, kursi makan, lemari pakaian, lemari hias, lemari 2 pintu, dan lemari 3 pintu di Mustika Jepara tahun 2022 berturut-turut sebesar Rp391.926811,67; Rp342.288.461,9; Rp237.360.665; Rp192.158.831,97; Rp192.095.664,83; dan Rp200.975.492,88 per tahunnya. Break Event Point (BEP) dalam produksi kursi tamu, kursi makan, lemari pakaian, lemari hias, lemari 2 pintu, dan lemari 3 pintu di Mustika Jepara pada tahun 2022 tercapai ketika produksi yang dihasilkan berturut-turut sebesar 11,70; 13,73; 5,48; 8,19; 8,18; dan 8,70 unit. Nilai ROI yang didapat PT. Mustika Jepara sebesar 15,97% pada tahun 2022. Harga pokok produksi kursi tamu, kursi makan, lemari pakaian, lemari hias, lemari 2 pintu, dan lemari 3 pintu berturut-turut yang hanya sebesar Rp19.238.707,4; Rp16.197.375; Rp22.450.362; Rp19.007.711,1; Rp19.001.462,8; Rp19.745.842,18
Keyword: Base Price, BEP, Production Cost, Chairs, Cabinets

Judul: Production Cost Analysis of Frame and Teak Door: Case Study PD Sumber Jati Makmur at Cirebon Regency, West Java
Abstrak: Industri furniture khususnya jati, memiliki prospek yang sangat baik untuk tumbuh dan berkembang karena sumber daya alam yang berlimpah serta nilai ekspor yang cukup tinggi mencapai 1,95 miliar US$ atau meningkat hingga 14,6% (Gunadi 2021). Penelitian ini bertujuan untuk menganalisis biaya produksi, Break Event Point (BEP), profitabilitas yang dinyatakan dalam persentase Return On Invesment (ROI), dan harga pokok kusen pintu dan daun pintu jati di PD Sumber Jati Makmur. Hasil penelitian menunjukkan bahwa biaya produksi kusen sebesar Rp 513.356,1 unit dengan biaya tetap Rp 194.797,9 per unit dan biaya variabel Rp 318.558,2 per unit. Biaya produksi daun pintu Rp 834.282 per unit dengan biaya tetap Rp 247.416,1 per unit dan biaya variabel Rp 586.865,5. BEP produk kusen pintu sebesar 111,3 unit per tahun dan daun pintu sebesar 102,6 unit per tahun. Harga pokok kusen pintu Rp 641.695,1 jauh dari harga jual sebenarnya yaitu Rp 800.000 dan daun pintu Rp 1.042.852 per unit dimana harga jual sebenarnya yaitu Rp 1.250.000. Return On Invesment (ROI) PD Sumber Jati Makmur sebesar 55,2% sehingga kegiatan produksi kusen dan daun pintu jati menguntungkan.
Keyword: Production Cost, Frame, Teak Doors, Best Price, Profitability

Judul: DNA Extraction and Characterization of Salmonella‟s Lytic Bacteriophage Genome
Abstrak: Bacteriophages are ubiquitous in nature and are known to proliferate wherever their bacterial hosts exist. Their virion particles can exist independently outside the host, however all phages are need their host to propagate. Several phages are highly specific to host cell surface receptors and any slight changes in structure results in little or no interaction between the phage and its host. Salmonella‟s phage isolated from sewage water where their bacteria exist. Three isolated of phages (phages 15, 19 and 38) were used in this research that concentrated using three types of methods. PEG/ NaCl method was firstly used to concentrate the phages based on their molecular weight to precipitate the phage particles during centrifugation. The second method was Scraping Plaque method. In this method, overlay plaques were scraped and collected in SM buffer to get phage concentrates. Enrichment method was the last method used to concentrate the phages. Phage genome isolation was done using two methods i.e. EDTA/SDS and Proteinase K methods. The first method was done using DNase I to eliminate bacterial DNA followed by SDS/EDTA treatment to release phages DNA and phenol-chloroform-isoamyl alcohol to extract the DNA. DNA was collected by isopropanol extraction. While in Proteinase K method, bacterial DNA was eliminated by DNase I and Proteinase K/SDS was used to degrade phages capsids and ethanol for DNA precipitation. The result showed that PEG/NaCl method resulted the highest phage concentration for phage 15 and phage 19, while scrapping method was the highest for phage 38. The quantity and purity of extracted DNA were low for PEG/NaCl and Scraping methods, while Enrichment method was higher indicated higher purity. Run on agarose gel electrophoresis of extracted DNA confirmed that Enrichment method results were the best while scraping and PEG/NaCl method was appeared smear background. Based on DNA genome size determination, the bacteriphages of Salmonella sp were comparable with other study of Salmonella phages. Phage P15 with genome size of 39,5 kb was close to phage epsilon15. Phage P19 with genome size of 41 kb was close to phage ST160. And phage P38 with genome size of 48,5 kb was close to phage Gifsy-1.
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