Judul: Hubungan Tingkat Penggunaan Smartphone Pada Remaja Dengan Interaksi Dalam Keluarga (Kasus Remaja Di Kelurahan Mulyaharja, Kecamatan Bogor Selatan, Kota Bogor)
Abstrak: Pada zaman modern seperti sekarang ini banyak yang bergantung pada kecanggihan teknologi media, salah satunya dengan memanfaatkan smartphone seperti yang digunakan oleh remaja. Banyak remaja yang terpengaruh oleh kecanggihan smartphone, sehingga penggunaan smartphone memberikan perubahan terhadap pola interaksi antara remaja dengan orang tua. Pada saat proses komunikasi berlangsung, gangguan yang membuat perhatian remaja menjadi teralih saat diajak berkomunikasi secara tatap muka atau sering terjadi miscommunication akibat keseruan dengan smartphone mereka masing-masing. Penelitian ini menganalisis keterkaitan antara hubungan tingkat penggunaan smartphone pada remaja dengan interaksi di keluarga. Penelitian dilakukan dengan menggunakan pendekatan kuantitatif yang didukung oleh data kualitatif dan menggunakan metode teknik accidental sampling untuk penarikan sampel. Hasil penelitian menunjukkan bahwa tidak terdapat hubungan yang signifikan antara tingkat penggunaan smartphone pada remaja dengan interaksi di keluarga.
Keyword: Interaksi keluarga, remaja, smartphone

Judul: Sikap dan Perilaku Remaja Desa dalam Menggunakan Telepon Seluler (Studi Kasus Remaja Desa Cihideung Ilir Kecamatan Ciampea Kabupaten Bogor Provinsi Jawa Barat).
Abstrak: This study aims to analyze the attitudes and behavior of mobile phone use in rural youth, to analyze the relationship of rural youth characteristics with their attitude towards mobile phone, to analyze the relationship of rural youth characteristics with their behavior in the use of mobile phone, and analyze the relationship of attitudes with the behavior of rural youth in the use of mobile phone. The study method were quantitative method using kuesioner and support by qualitative method. The results of this study is rural youth tend to have a positive attitude toward mobile phone and behavioral levels high enough in using the mobile phone. There is a close and significant relationship between the level of media and advertising exposure with rural youth behavior in using the phone. In the meantime, there is no close and significant relationship among rural youth attitudes toward the phone with their behavior in using the phone
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Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable