Judul: Isolasi Cendawan Mikoriza Arbuskula pada Tanaman Berkayu Asal Pulau Bangka
Abstrak: Arbuscular Mycorrhizal Fungi (AMF) associate with a variety of plants including forest plants. Research on AMF in forestry in Indonesia is limited, especially on woody plants grown in Bangka Island. Therefore, the aim of this research was to isolate and identify AMF associate with woody plants in Bangka Island grown in post mining area. The Soil samples derived from 8 trapping pots of 4 years woody plants from Bangka Island grown in green house were used as spore inoculum source. Spore extraction from the soil used wet sieving and decanting method. Fungal identification carried out based on the method of “Manual for The Identification of VA Mycorrhizal Fungi”, and fungal isolation used pot culture with Pueraria javanica as a host plant. Fungal structures within the root were analyzed after root staining with trypan blue was conducted. There were 18 spore morphotypes observed which belong to 5 types of Acaulospora and 13 types of Glomus. Seven single spore pot cultures were successfully done from species of Acaulospora sp.1, Acaulospora sp.4, Acaulospora sp.5, Glomus sp.2, Glomus sp.3, Glomus sp.6, and Glomus sp.11. AM fungal colonization observed within the root was arum type which consisted of internal hyphae, asbuscules and vesicles.
Keyword: Bogor Agricultural University (IPB), Bangka Island., Glomus, Acaulospora, woody plants, AMF

Judul: Cendawan Mikoriza Arbuskula pada Sawo Duren (Chrysophyllum cainito) di Kampus IPB Darmaga
Abstrak: Arbuscular mycorrhizal fungi (AMF) are soil fungi that symbiosis with the plant roots. Research about AMF on fruit plants are rarely reported. The objectives of this research are to identify AMF spore and to detect the structure of arbuscular mycorrhiza (AM) on star apple. AMF spore was isolated from the star apple rhizosphere soil following the wet sieving and decanting method and centrifugation, and then identified based on shape, color, size, and spore wall ornamentation. The AM structure was observed on colored root using trypan blue. The result of the research showed that AMF was associated with star apple. The structures of AM in the roots of star apple were internal hyphae, coiled hyphae, and vesicles. The percentage of AM infection in the star apple’s root was 12-31 %. The AMF spores found in the star apple rhizosphere were Acaulospora bireticulata, A. scrobiculata, Ambispora cf. fecundisporum, Funneliformis cf. geosporum, Glomus cf. aggregatum, Glomus cf. microaggregatum, Glomus sp. 1, and Glomus sp. 2.
Keyword: Acaulospora, Ambispora, arbuscular mycorrhiza, Chrysophyllum cainito, Funneliformis, Glomus

Judul: Studi Kasus Liver Neoplasia pada Anjing Shih Tzu
Abstrak: Liver neoplasia is the excessive and uncontrolled replication of hepatocytes that forms nodules visible in ultrasonography. The purpose of this study is to study and discuss the characteristic, diagnosis and treatment of liver neoplasm through the interpretation of ultrasonography and hematology in a Shih Tzu dog. Ultrasonography is conducted with a Chison Q8 ultrasound machine with a 5 to 7 MHz frequency convex probe on lateral recumbency. The blood cells, liver enzymes and nodules sizes were the main parameters in this study. The results in the period of three years from 2021 to 2023 were interpreted and supported with secondary data sources. The Shih Tzu dog is 14 years old in the year 2023 with a body weight of 5.21kg. No obvious clinical signs were observed during previous examinations. However, lethargy, vomiting and anorexia were recorded in its latest examination. Thrombocytosis, granulocytosis, high liver enzymes and hypoglycemia, which are some of the neoplastic clinical manifestations were also presented by this patient. Besides, liver neoplasm, spleen neoplasm and biliary sludge were the sonogram findings of this patient. The liver and spleen neoplasm was observed stable, while the biliary sludge volume fluctuated. The treatment strategy recorded is Ondansetron, Samylin®, Ornipural and Ursodeoxycholic acid.
Keyword: Blood biochemistry profile, Hematology, Liver Neoplasia, Shih Tzu, Ultrasonography