Judul: Identifikasi Keragaman Gen Follicle Stimulating Hormone Receptor (FSHR|AluI) pada Spesies Sapi Bos javanicus, Bos taurus, dan Bos indicus dengan Metode PCR-RFLP.
Abstrak: Follicle Stimulating Hormone Receptor (FSHR) gene is a very important in reproduction trait. FSHR is required by Follicle Stimulating Hormone (FSH) to carry out on target tissues. The purpose of this study was to identify polymorphism of the Follicle Stimulating Hormone Receptor gene in Bos javanicus, Bos taurus, and Bos indicus Cattle. Blood samples used 207 samples consisting of Bos javanicus (Bali Cattle) 59 heads, Bos taurus 137 heads (Limousin 57 heads, Simmental 50 heads, Angus 6 heads, and Holstein Friesian (FH) 24 heads), and Bos indicus 11 heads (Brahman 9 heads and Ongole 2 heads). The polymorphism site analysis from digestion with AluI restriction enzyme allowed the genotype identification. AluI restriction enzyme cut the base position at AG|CT in 193 and 243 bp. Genotyping FSHR gene produced two alleles, namely C and G alleles. These allele produced three genotypes respectively, there are CC (243 and 63 bp), GG (193, 63, and 50 bp), and CG (243, 193, 63, and 50 bp). The highest G allele frequency was found in Bos javanicus (Bali cattle), but the lowest in Bos taurus. The highest C allele frecuency was found in Bos taurus. The value of heterozigosity was the highest in Bos indicus and the lowest in Bos javanicus. The result of this study showed that the allele of FSHR gene in Bos taurus and Bos indicus were polymorphic, while in Bos javanicus was monomorphic.
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Judul: Identifikasi keragaman Gen Growth Hormone Receptor (GHR AluI) pada Sapi Friesian Holstein di BIB Lembang, BBIB Singosari, dan BET Cipelang.
Abstrak: Growth hormone receptor (GHR) is one factor affecting animal growth. GHR is required by growth hormone (GH) to carry out its effect on target tissues. The objective of this study was to identify polymorphism of the Growth Hormone Receptor gene in Holstein Friesian (HF) dairy cattle and beef cattle. Genotyping was performed on 126 animals. These consisted of HF 89 heads from BIB Lembang (17), BBIB Singosari (32), and BET Cipelang (40); as well as for 37 heads of four breeds of beef cattle including for Simmental (13), Limousin (14), Brahman (5), and Angus (5) for a comparison. Varian genetic of the GHR gene was identified by PCR-RFLP method using AluI restriction enzyme cutting the base position at AG|CT. Single nucleotide polymorphism (SNP) had been found in exon 10, coding for the cytoplasmic domain of GHR, which was located at position 81 bp (A/G) induced amino acid substitutions Ser/Gly. The product of amplification of GHR was 298 bp. Genotyping the GHR gene produced two alleles (A and G) resulting three genotypes (AA, AG and GG). The genotype AA was dominant in HF cattle from all locations, and also in Brahman and Limousin cattle from BET Cipelang. All of HF dairy cattle were in Hardy-Weinberg disequilibrium (χhit 2>χ0,05 2), in contrast, Simmental and Limousin were in Hardy-Weinberg equilibrium. The Hardy-Weinberg in Brahman and Angus values could not be calculated. Heterozigosity values in HF and beef cattle were low (ho<he). In beef cattle, Angus had highest heterozigosity value (0,600), while Brahman had the lowest heterozigosity value (0.000). The conclusion was that the variability of GHR|AluI gene can be used as a basic information for selection in growth caracteristic in the HF and beef cattle. Keywords: Beef cattle, Genetic polymorphism, GHR|AluI gene,
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Judul: Kinerja model pengering bangunan tembus cahaya dari plastik tahan UV untuk pengeringan buah kopi
Abstrak: Kopi merupakan komoditas ekspor yang memerlukan pengeringan sebelum dikonsumsi, sehingga kemampuannya dapat disimpan dalam waktu lama sangat menentukan mutu dan nilai ekonominya. Penyediaan sarana pengeringan yang memadai sangat dibutuhkan untuk mencapai tujuan tersebut. Sebuah model bangunan tembus cahaya untuk pengeringan kopi skala 1.5 ton sudah dirancang dan diuji coba. Bangunan tersebut berukuran 6 m x3 m, tinggi 2.46 m terbagi menjadi dua. yaitu atap 0.56 m dengan sudut kemiringan 32.4" dan dinding 1.9 m membentuk sudut kemiringan 85.6" terhadap lantai. Plastik tahan UV menutupi atap dan dindingnya. Penelitian ini bertujuan menguji kinerja model pengering bangunan tembus cahaya pada kapasitas maksimum. Percobaan dilakukan dengan mengeringkan kopi pada ketebalan 30 cm dan 40 cm (kapasitas penuh) dibantu blower 0.5 11P, 220 volt untuk mengalirkan udara pengering di dalam bangunan tembus cahaya dan mengeluarkan uap air bahan yang telah diuapkan dari bangunan tersebut. Hasil percobaan memperlihatkan bahwa penutup transparan mampu memanfaatkan 70% radiasi matahari yang jatuh di permukaannya. Pada kondisi radiasi termanfaatkan rata-rata sebesar 435.1 W/m². laju aliran udara sebesar 2520...
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