Judul: Antimicrobial Activity of Bacillus sp. Lts 40 against Ralstonia solanacearum, Pseudomonas syringae pv. glycines, and Pseudomonas fluorescens.
Abstrak: The using of chemical substance as biocontrol could leave the residue which acumulated on environment. Therefore, it is necessary to have more safety and non residual substances. Determination of antimicrobial activity of Bacillus sp. Lts 40 isolated from shirmp pond was conducted in vitro experiments. This isolate was assayed their inhibition activity to the growth of six isolates (Escherichia coli, Staphylococcus aureus, Pseudomonas fluorescens A, Pseudomonas fluorescens B, Ralstonia solanacearum, and Pseudomonas syringae pv. glycines) of plant pathogenic bacteria using spread plate method. The results showed that the isolate was able to inhibit the growth of all indicator bacteria. The higher inhibiting activity was found on Ralstonia solanacearum.
Keyword: 

Judul: The potency of antifungal bioactive compound producing bacteria to control rice sheath blight disease
Abstrak: The objectives of this research were to (i) screen some potential bacteria as biological control agents of the sheath blight disease caused by R. solani, (ii) identify character of the selected bacteria strains as biological control agents, and (iii) characterize the antifungal activities of the antifungal bioactive compound produced by the selected strains against R. solani. The antagonist bacteria were isolated from rhizospheric soil, rice plant, and pond water collected from several locations. Screening of bacteria strains on the growth inhibition of R. solani was conducted in the laboratory, while the selection of bacteria for their effectiveness to suppress sheath blight disease were done in the screen house. The result of this research showed that there are three bacterial isolates which antifungal activity significantly suppressed the rice sheath blight disease, i.e. TT47, SS19, and BR2 with suppression ability of 79,6, 56,4, and 49,47%, respectively. Molecular analysis of partial sequence of 16S rRNA showed that SS19, TT47, and BR2 isolates were Serratia marcescens, Ralstonia pickettii, and Bacillus subtilis, respectively. These biocontrol agents are not phythotoxic, produce siderophore, and phosphate-solubilizing. S. marcescens SS19 and R. pickettii TT47 were chitin-degrading bacteria but B. subtilis BR2 was not. The filtrate from fermentation broth of the R. pickettii TT47 and B. subtilis BR2 inhibited the growth of R. solani. The antifungal bioactive compound was relatively stable on pH range from acidic to basic (pH 4, pH 7, and pH 10). The optimum activity of antifungal bioactive compound was at room temperature (25-27 0C) and tend to decrease at higher temperature (60, 80. 100, and 121 0 Key words: The rice sheath blight, antifungal bioactive compounds, Serratia marcescens, Ralstonia pickettii, Bacillus subtilis. C). The antifungal activity of hexane extract of R. pickettii TT47 and ethyl acetate extract of B. subtilis BR2 in vitro revealed significant growth inhibition to R. solani, respectively by 86,3% and 77,8%. MIC of hexane extract and ethyl acetate extract were 0,1 mg/l and 10 mg/l respectively, and both extracts are not phythotoxic against the rice seedling.
Keyword: Bacillus subtilis, Rhizoctonia solani Kühn, Plant diseases

Judul: Millennial and Zoomer Generation Consumers’ Purchasing Decisions and Preferences at Coffee Shops in Madiun City
Abstrak: Perkembangan zaman melahirkan generasi baru dengan preferensi minuman kopi yang diduga berbeda dari generasi sebelumnya. Ketersediaan pilihan kedai kopi telah memperluas alternatif yang dapat dipertimbangkan oleh konsumen dalam keputusan pembelian. Sebagai salah satu kedai kopi, IKIO Coffee dapat melakukan segmentasi pasar berdasarkan karakteristik tertentu. Penelitian ini bertujuan untuk mengidentifikasi perbedaan karakteristik konsumen generasi Millennial dan Zoomer serta melakukan segmentasi konsumen berdasarkan karakteristik demografis, perilaku, dan psikografis dalam pembelian minuman kopi di kedai-kedai kopi di Kota Madiun. Data primer dikumpulkan melalui kuesioner yang diisi oleh 180 responden pada periode April - Juli 2022. Metode pengambilan sampel menggunakan teknik judgmental sampling dengan kriteria kelompok usia. Analisis yang dilakukan meliputi analisis deskriptif, uji T sampel bebas untuk analisis perbedaan, dan analisis cluster menggunakan metode K-Means Clustering. Hasil penelitian menunjukkan bahwa konsumen generasi Millennial dan Zoomer cenderung didominasi oleh perempuan, angkatan kerja, memiliki tujuan mengisi waktu luang, mencari informasi melalui media sosial, dan memberikan prioritas pada cita rasa. Terdapat perbedaan signifikan antara konsumen Millennial dan Zoomer dalam preferensi kedai kopi terkait media promosi, fasilitas WIFI/stopkontak, fasilitas hiburan live music, proses pemesanan, dan proses pembayaran. Berdasarkan preferensi terhadap atribut kedai kopi, segmentasi konsumen terbagi menjadi tiga segmen, yaitu pecinta kopi, petualang rasa kopi, dan konsumen yang mengedepankan kualitas minuman kopi. Implikasi manajerial bagi IKIO Coffee dan kedai-kedai kopi lainnya adalah mengarahkan strategi pemasaran lebih khusus pada segmen konsumen petualang rasa untuk generasi Zoomer, serta mengutamakan inovasi dan diferensiasi dalam menyajikan minuman kopi berkualitas untuk generasi Millennial.
Keyword: coffee shops, consumer preferences, generation Millennial, generation Zoomer, segmentation