Judul: Pengujian virus Newcastle disease gen VII isolat lapang sebagai virus standar pada uji tantang vaksin
Abstrak: Penelitian ini dilakukan untuk mengetahui standar virus Newcastle Disease Gen VII isolat lapang sebagai virus tantang pada pengujian vaksin. Suspensi virus yang telah diuji HA sebelumnya dititrasi dengan uji Embryo Infectious Dose 50 (EID menggunakan telur embrio tertunas (TET) SPF umur 10 hari sebanyak 30 butir. Eji Chicken Lethal Dose30 (CLD50) dilakukan untuk mengetahui titer virus yang mampu menyebabkan kematian sebesar 50% populasi ayam. Uji CLD50 menggünakan 25 ekor day-old-chicken (DOC) yang dibagi menjadi 5 kelompok. Virus i encerkan dari 10 sampai 10%. Pada pengenceran 10 sampai 10%, virus ND dinfeksikan kepada setiap kelompok ayam melalui tetes mata dan intranasal sebanyak 0.1 ml/ekor. Uji hemaglutinasi menunjukkan bahwa cairan alantois yang dipanen memiliki titer virus yang tinggi yaitu 512 HAU. Virus infektif ND yang dititrasi dengan uji EID50 menunjukkan titer virus sebesar 10° EID50/0.1 ml atau 100 ELD50/ml dan titer virus pada uji CLD50 menunjukkan titer virus sebesar 10648 CLD 20.1 ml atau 1074 CLD50/ml. Hasil ini dapat disimpulkan bahwa isolat ND Gen Vil sangat sesuai digunakan sebagai virus standar pada uji tantang vaksin…
Keyword: CLD50 test, EID50 test, HA test, Newcastle disease virus gene VII

Judul: Pengujian Virus Newcastle Disease Gen VII Isolat Lapang Sebagai Virus Standar Pada Uji Tantang Vaksin
Abstrak: This study was conducted to determine the standard of Newcastle disease virus Gene VII field isolate as a virus challenge to vaccine test. A virus suspension that has previously been tested by HA test then titrated with EID50 using 30 Specific Pathogen Free (SPF) of 10 day old chicken embryonated eggs. In addition, chicken lethal dose50 (CLD50) test were used to determine the virus titer that could cause 50% death of the chicken population. The CLD50 test was used 25 day-old-chickens (DOC) divided into 5 groups. The virus was diluted from 10-1 to 10-8. The 104 to 10-8 dilution of NDV was infected to every group of chicken via eyedrop and intranasal with 0.1 ml/chicken dosage. Haemagglutination test showed that the allantoic fluid were contain high titers of NDV is 512 HAU. The infective NDV that had been titrated by EID50 test showed the virus titers is 109 EID50/0.1 ml or 1010 EID50/ml and the virus titers of CLD50 test e.i 106.48 CLD50/0.1 ml or 107.48 CLD50/ml. These results can be concluded that the NDV Gene VII field isolates were most appropriate to be used as a standard virus to vaccine test
Keyword: Newcastle Disease Virus Gene VII, HA tes, EID50 test, CLD50 test

Judul: Penggunaan Teorema Homeomorphy 2-Manifold dan Teorema Euler Poincare pada Torus 𝑇�� dan Simplicial Complex 𝐾��
Abstrak: Two dimensional topological spaces are said to be homeomorphic if they have the same topological invariant, where one of topological invariant used is an Euler characteristic. Homeomorphy 2-Manifold’s Theorem and Euler Poincare’s Theorem are used to distinguish two topological spaces. Homeomorphy 2-Manifold’s Theorem uses Euler characteristic to identify two dimensional topological spaces. Euler Poincare’s Theorem is an alternative way to find Euler characteristic with Betti number, which is topological invariant as well. The objective of this paper is to investigate the homeomorphism of torus 𝑇����� and simplicial complex 𝐾�����. Topological space torus and simplicial complex 𝐾����� have the same Euler characteristic, which is two. Based on Homeomorphy 2-Manifold’s Theorem topological space torus 𝑇����� and simplicial complex 𝐾����� is homeomorphic.
Keyword: simplicial, torus, Euler, homeomorphism, topology