Judul: Evaluasi Kinerja Sistem Distribusi Air Pada PDAM Tirta Pakuan di Perumahan Griya Melati, Bogor, Jawa Barat
Abstrak: Water is one of the basic requirement for human being. It means that maintenance and menagement of water distribution system gets the first priority. The objective of this research was to evaluate performance of PDAM Tirta Pakuan water distribution system in Griya Melati recidence. The analysis was held based on questioner and Epanet 2.0. Analysis of network capability’s performance was done, including quantity, pressure, continuity, and quality, and also costumer satisfaction about PDAM performance. Based on costumer satisfaction survey about water physical quality parameters (i.e. taste, color and smell), the water was good, even sometime its smell like chlorine. The comparison between water use measurement and Epanet 2.0 simulation showed that PDAM was succeed to fulfill costumer demand even the heads and discharges were fluctuated. The highest headloss on pipe 19 was 0.072 m because the changed of pipe’s diameter from 100 mm to 50 mm so the velocity was increasing. PDAM has been distributing water for 24 hours so the water were always available for customers. Based on water quality test in PDAM laboratory, the result was suitable with Permenkes RI. No. 492/Menkes/Per/IV/2010.
Keyword: clean water, costumer, Epanet 2.0, performance, water distribution system

Judul: Analisis Sistem Distribusi Air Bersih di Perumahan Ciomas Permai, Kabupaten Bogor, Jawa Barat.
Abstrak: Sistem distribusi air bersih sering bermasalah dalam hal kuantitas, tekanan, kontinuitas dan kualitas air. Penelitian ini bertujuan untuk menganalisis sistem distribusi air bersih PDAM Tirta Kahuripan, Kabupaten Bogor di Perumahan Ciomas Permai. Penelitian dilakukan dengan menggunakan data primer dan data sekunder. Analisis sistem distribusi air bersih dilakukan dengan menggunakan aplikasi EPANET 2.0. Perumahan Ciomas Permai termasuk ke dalam zona 6 PDAM Tirta Kahuripan. Berdasarkan hasil penelitian didapatkan bahwa kualitas air yang didistribusikan telah sesuai dengan standar yang berlaku dan kontinyu selama 24 jam walaupun terdapat perbedaan debit yang signifikan saat jam puncak. Berdasarkan pengukuran pada hari Minggu dan Senin besarnya debit minimum sebesar 14.4 l/det dan 13.8 l/det, melebihi kebutuhan pelanggan sebesar 7.34 l/det, sedangkan tekanan berkisar antara 0.7 – 1.35 bar. Pada simulasi distribusi air bersih menggunakan EPANET 2.0 didapatkan bahwa kecepatan air dan headloss berdasarkan simulasi tidak memenuhi persyaratan yang berlaku.
Keyword: debit, EPANET 2.0, headloss, kualitas air, tekanan

Judul: Cloning and Structure Prediction of C-Type Lectin 19 (CTL-19) from Silkworm (Bombyx mori L.)
Abstrak: Bombyx mori L. is a highly significant insect due to its silk production affects several industries. Unfortunately, B. mori farming has recently experienced mortality issues caused by pathogenic infectious diseases. Despite the extensive study of innate immunity of B. mori, information regarding the molecular functions and mode of actions of C-type lectin 19 (CTL-19) in the innate immunity of B. mori remains limited. Therefore, this study aimed to predict the structure of the B. mori CTL-19 also clone and produce the recombinant protein to better understand its role in the silkworm's innate immunity. The open reading frame (ORF) of the CTL-19 gene (945bp) was successfully amplified and cloned to pGEM®-T Easy Vector. The mature protein consists of 294 amino acids with 2 CRDs, 9 conserved cysteine, and two specific carbohydrate binding motifs. Protein threading demonstrated to be superior in order to predict the ligand binding site in comparison to homology modelling. The predicted molecular weight is 33.26 kDa, with pI value of 5.70. Furthermore, molecular docking analysis reveal that BmCTL-19 have ability to bind to various carbohydrate residues. The BmCTL-19 gene will be sub clone into an appropriate expression vector and produce purified recombinant protein to evaluate its properties.
Keyword: Innate immunity, Carbohydrate Binding Motif, Homology modelling, Protein Threading, Molecular docking