Judul: Analysis of Land Cover Changes for Mangrove Vegetation and Coastlines in Indramayu Regency, West Java
Abstrak: Mangrove merupakan individu maupun komunitas tumbuhan yang hidup di kawasan pesisir. Wilayah pesisir merupakan ekosistem peralihan antara laut dan daratan. Kondisi tersebut menyebabkan wilayah pesisir mendapatkan tekanan dari darat sehingga pada akhirnya memberikan dampak pada ekosistem pantai. Kabupaten Indramayu merupakan salah satu wilayah di pantai utara Jawa Barat yang mengalami kerusakan paling parah di antara seluruh kabupaten di wilayah pesisir utara Jawa Barat. Penelitian ini bertujuan untuk mengetahui perubahan tutupan lahan vegetasi mangrove, garis pantai, dan penyebab terjadinya perubahan luasan tutupan lahan vegetasi mangrove dan garis pantai tahun 1990-2020 di Kabupaten Indramayu. Penelitian ini dilakukan pada bulan Maret-Juni 2021 di Kecamatan Losarang, Cantigi, Pasekan, dan Indramayu. Hasil penelitian menunjukan perubahan yang signifikan dari setiap periode tahun penelitian. Perubahan tutupan lahan vegetasi mangrove terjadi karena adanya konversi tutupan lahan vegetasi mangrove menjadi tambak. Sedangkan perubahan garis pantai terjadi karena dari faktor alam dan faktor manusia., Mangroves are individuals and plant communities that live in coastal areas. The coastal area is a transitional ecosystem between sea and land. This condition causes the coastal area to come under pressure from the land, impacting the coastal ecosystem. Indramayu Regency is one of the areas on the North Coast of West Java that experienced the most severe damage among all districts in the North Coast of West Java. This study aims to determine changes in land cover of mangrove vegetation, coastlines and the causes of changes in mangrove vegetation and coastlines from 1990-2020 in Indramayu district. This research was conducted in March-June 2021 in Losarang, Cantigi, Pasekan, and Indramayu Districts. The results showed a significant change in each period of the research year. Changes in land cover of mangrove vegetation occur because of the conversion of mangrove vegetation land cover into ponds. Meanwhile, shoreline changes occur due to natural and human factors.
Keyword: garis pantai, landsat, mangrove, shoreline

Judul: Study of Changes in Mangrove Area and Density Using Satellite  Imagery in Indramayu, West Java
Abstrak: Hutan mangrove merupakan salah satu tipe hutan yang memiliki karakteristik berbeda dengan formasi hutan lainnya. Mangrove memiliki fungsi ekologi dan ekonomi. Mangrove terus mengalami penurunan secara kualitas dan kuantitas karena berbagai faktor. Kabupaten Indramayu merupakan salah satu wilayah persebaran mangrove di Indonesia yang mengalami penurunan. Penelitian ini bertujuan mengetahui perubahan luasan dan kerapatan mangrove dari tahun 1990- 2020 dan penyebab terjadinya perubahan luasan dan kerapatan mangrove di Kabupaten Indramayu. Penelitian ini dilakukan pada bulan Maret-Juni 2021 di Kecamatan Losarang, Cantigi, Pasekan, dan Indramayu, Kabupaten Indramayu, Jawa Barat. Data citra dianalisis menggunakan model Forest Canopy Density. Hasil penelitian menunjukan perubahan yang beragam dari setiap periode tahun penelitian. Mangrove di Indramayu mengalami penurunan terbanyak pada tahun 2000. Perubahan tersebut terjadi karena banyaknya konversi lahan menjadi tambak dan pemanfaatan batang mangrove yang berlebihan. Kesadaran masyarakat terus meningkat pada tahun 2010 dan 2020 dilihat dari luasan dan tingkat kerapatan mangrove yang terus bertambah.
Keyword: dynamic, forest canopy density, mangrove

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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