Judul: Father's Perception, Child Educational Facilities, Child-Parent Communication, and Motivation for Continuing School of Adolescence Circular Migrant Worker Families
Abstrak: Anak pada keluarga pekerja migran sirkuler dengan kondisi hubungan orang tua dan anak yang terpisah memiliki tantangan tersendiri agar terus termotivasi melanjutkan sekolah. Penelitian ini bertujuan untuk menganalisis pengaruh persepsi ayah mengenai pendidikan anak, penyediaan fasilitas pendidikan anak, dan komunikasi anak-orang tua terhadap motivasi melanjutkan sekolah pada remaja keluarga pekerja migran sirkuler. Penelitian ini merupakan penelitian kuantitatif dengan metode penarikan contoh dengan non-probability sampling melalui teknik snowball sampling. Jumlah responden dalam penelitian ini adalah 40 anak dan 40 ayah menggunakan kuesioner melalui Google Form. Hasil penelitian menunjukkan pendidikan terakhir ayah, penyediaan fasilitas pendidikan anak, komunikasi anak-ibu berhubungan positif signifikan dengan motivasi melanjutkan sekolah. Pendidikan terakhir ayah dan komunikasi anak dengan ibu berhubungan signifikan positif dengan penyediaan fasilitas pendidikan anak. Penyediaan fasilitas pendidikan anak, frekuensi kepulangan, dan komunikasi anak-ibu memiliki pengaruh positif signifikan terhadap motivasi melanjutkan sekolah. Sementara itu, jumlah anak berpengaruh signifikan negatif terhadap motivasi anak dalam melanjutkan sekolah., Children in circular migrant worker families with separate parent-child relationships have challenges to keep them motivated to continue school. This study aims to analyze the effect of fathers' perceptions of children's education, provision of children's educational facilities, and parent-child communication on the motivation to continue schooling in adolescent families of circular migrant workers. This research is quantitative research with a sampling method with non-probability sampling through the snowball sampling technique. This study’s respondents were 40 children and 40 fathers using a questionnaire via Google Forms. The results showed that the father's last education, children's educational facilities, and child-mother communication had a significant positive relationship with motivation to continue school. The father's last education and the child's communication with the mother were significantly positively related to the child's educational facilities. Children's educational facilities, frequency of return, and child-mother communication significantly positively affect motivation to continue school. Meanwhile, the number of children significantly negatively affects children's motivation to continue school.
Keyword: educational facilities, circular migrant worker families, motivation to continue school, perception of education, parent-child communication

Judul: The Influence of Paternal Self-Efficacy and Mother Support on Father Involvement of Circular Migrant Worker
Abstrak: Peran ayah dalam pengasuhan anak sangat menentukan perkembangan anak. Bagi ayah pekerja migran sirkuler yang tinggal jauh dari keluarga karena bekerja di luar daerah akan lebih sulit untuk menjalankan peran pengasuhan. Penelitian ini bertujuan menganalisis pengaruh paternal self-efficacy dan dukungan ibu terhadap keterlibatan ayah dalam pengasuhan pada pekerja migran sirkuler. Penelitian ini menggunakan pendekatan kuantitatif dengan desain penelitian eksplanatori. Pengambilan data dilakukan secara daring menggunakan Google Form dengan jumlah 41 responden ayah yang berstatus sebagai pekerja migran sirkuler. Data diolah menggunakan Microsoft Office Excel dan dianalisis menggunakan SPSS. Hasil penelitian menunjukkan bahwa usia ayah berhubungan positif signifikan dengan paternal self-efficacy, sedangkan paternal self-efficacy, dukungan ibu, dan keterlibatan ayah dalam pengasuhan saling berhubungan positif signifikan. Selanjutnya, pendidikan terakhir ayah berhubungan negatif signifikan terhadap paternal self-efficacy dan dukungan ibu. Hasil penelitian juga menunjukkan bahwa paternal self-efficacy dan dukungan ibu berpengaruh positif signifikan terhadap keterlibatan ayah dalam pengasuhan pada pekerja migran sirkuler., Fathering plays a very important role in child development. The circular migrant worker fathers who live in different region from their families will have many difficulties to carry out the parenting role. This study aimed to analyze the influence of paternal self-efficacy and mother support on father involvement in circular migrant worker. This study used a quantitative approach with an explanatory research design. Data collection was done online using Google Form with a total of 41 respondents of circular migrant worker fathers. The data was processed using Microsoft Office Excel and analyzed using SPSS. The results showed that the father's age was significant positively correlated with paternal self efficacy, while paternal self-efficacy, mother support, and father involvement have a significant positive relationship with each other. Furthermore, the education level of father has significant negative relationship with paternal self-efficacy and mother support. The results also show that paternal self-efficacy and mother support have a significant positive effect on father involvement.
Keyword: dukungan ibu, keterlibatan ayah, paternal self-efficacy, pengasuhan, pekerja migran sirkuler

Judul: Characterization and Expression of Tomato infectious chlorosis virus Coat Protein Gene in Escherichia coli
Abstrak: Tomato infectious chlorosis virus (TICV) is a member of Crinivirus genus. TICV infects tomato crop in Garut and Cipanas, West Java Indonesia. Tomato initially showed bright interveinal yellowing symptoms. The coat protein gene (CP-TICV) was amplified by RT-PCR from total RNA extracted from infected tomato leaves and the amplified fragment was cloned and completely sequenced. The fragment was subsequently subcloned into the pET-21b expression vector. The recombinant plasmid was transformed to Escherichia coli strain BL21(DE3)pLysS to express the coat protein. The coat protein fused to a 6xhistag, was purified by affinity chromatography using a NiNTA spin column. The identity of the purified protein was confirmed by SDS-PAGE. In this experiment, 792 bp of CP-TICV gene of TICV virus has been successfully cloned, sequenced, and expressed in E. coli. Based on nucleotide sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain strain and 99% identity to North America, France, and California strains and based on amino acid sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain, North America, France strains and 99.2% identity to California strain. CP-TICV showed over expressed in E. coli when it is induced with 1 mM IPTG and incubated at 37oC. Purified CP-TICV-Histag recombinants protein sized 29 kDa based on SDSPAGE analysis.
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