Judul: Fisiologi Pertumbuhan dan Pembungaan Tanaman Manggis (Garcinia mongostana L.) Asal Biji dan Sambungan
Abstrak: Mangosteen trees from seedling grow faster, but have longer juvenile period than the grafted trees. In contrast, grafted trees grow slower, but begin to flower earlier. The research was conducted to study the physiology of growth and flowering of seedling mangosteen.
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Judul: Studies on anatomical and physiological aspects of mangosteen graftings with several relatives of Clusiaceae.
Abstrak: Pertumbuhan pohon manggis (Garcinia mangostana L.) dikenal sangat lambat. Usaha mempercepat pertumbuhan tanaman sejak dari pembibitan dilakukan dengan bibit-semai mau pun bibit-sambung. Pertumbuhan vegetatif bibitsemai di lapangan lebih hat, tetapi bibit-sambung lebih cepat berbuah. Penelitian ini mengkaji faktor-faktor penyebab terhambatnya pertumbuhan bibit-sambung dengan mengamati aspek-stspek anatomi dan fisiologi pada saat penyembuhan luka dan pertautan jaringan pembuluh. Percobaan dilaksanakan dari bulan Maret 2000 hingga November 200 1 bertempat di Tarnan Buah Mekarsari, Cileungsi dan Kebun Citra Ciptaku di Cijeruk, Bogor. Analisis laboratorium dilakukan di Balitbang Botani dan Balitbio Tanaman Pangan di Bogor, dan BATAN di Jakarta
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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