Judul: Persepsi, sikap dan prilaku masyarakat sekitar hutan terhadap nilai sumberdaya hutan : Studi kusus di Desa Cihayawar Kecamatan Nagrak Kabupaten Sukabumi
Abstrak: Masyarakat sekitar hutan memiliki keterkaitan yang erat dengan hutan dan lingkungan dan adanya ketergantungan antara masyarakat dan sumberdaya hutan. Pemberdayaan masyarakat menjadi hal yang penting dan menentukan dalam upaya menjaga kelestarian hutan. Informasi mengenai keadaan dan kondisi masyarakat menjadi hal yang perlu diperhatikan seperti persepsi, sikap dan perilaku masyarakat yang diharapkan selaras dengan prinsip-prinsip kelestarian. Persepsi, sikap dan perilaku memiliki keterkaitan satu sama lain dan saling mempengaruhi. Persepsi akan mempengaruhi sikap dan sikap akan mempengaruhi perilaku. Namun hubungan yang ada tidak selalu konsisten dan dipengaruhi faktor-faktor lain. Persepsi responden terhadap sumberdaya hutan di Desa Cihanyawar yang merupakan desa yang berbatasan langsung dengan hutan produksi (Perhutani) dan Taman Nasional Gunung Gode Pangrango merasa memiliki keterkaitan secara langsung dalam segi ekonomi dan ekologi. Manfaat ekonomi yang dirasakan responden terkait dengan adanya lahan tumpang sari sebagai lahan garapan dan hasil hutan lainnya.
Keyword: forestry, persepsi

Judul: Persepsi, Sikap, Perilaku Masyarakat Sekitar Hutan terhadap Keberadaan hutan Penelitian Haurbentes (Studi Kasus desa Jugalaya, RPH Jasinga, BKPH Jasinga, KPH Bogor
Abstrak: The Society around forest has a strong correlation with the forest and the environment; also there is dependence between the society and forest resources. Making efficient use of the society becomes an important and determined thing in order to keep the preservation of the forest. The information about the situation and condition of the society becomes the things that need to be concerded. Such as perception, attitude and behaviour of the society which are expected to be in accordance with the principle of the conservation. Perception, attitude and behaviour prossess a connection one another and influence each with production forest (Perhutani)and Research Forest haurbenties, is they feel that they have a direct connection in economics and ecology. The economics use that the respondents feel interrelated with the existence of land as an area to produce and others forest products. This research intends to know the perception, attitude and behaviour of the society towards the forest in keeping the preservation of the forest, to know the factors that influence the perception, attitude and behaviour towards forest resources, also to know the relation among the perception, attitude and behaviour of the society it self
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Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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