Judul: Pengaturan Laju Oksigenasi untuk Meningkatkan Ketahanan Sel Rekombinan Escherichia coli pada Kultivasi Etanol Tinggi
Abstrak: The cell tenacity improvement of Escherichia coli recombinant under high ethanol culture conditions by arrangement oxygenation rate was investigated in this study. Dry cell weight, glucose, and acetate production were analysed to show the effect of this treatment. E. coli cells were grown in 25 and 50 ml of medium with 250 rpm agitation which shown the high and low oxygenatian rate. Cultivation taken a place during 24 hours aerobically with ethanol addition into the culture time of t = 6 h. Improvement of oxygenation rate under high ethanol culture condition could increase the cell growth until 56%. Improvement of oxygenation rate also decreased the acetate production from 6.27 g/l to 4.45 g/l. This acetate production was ralated with overflow metabolism phenomenon. Improvement of oxygenation rate intent on improvement TCA cycle activity for increasing cell growth and repairing cell structure by producing glutamate. The glutamate (2 g/l) was added to confirm the effect of improvement oxygenation rate on cell tenacity. Improvement of oxygenation rate and glutamate addition resulted in carbon flux in TCA cycle was over capacity. It caused the carbon was accumulated and was used for acetate production
Keyword: Bogor Agricultural University (IPB), oxygenation rate, overflow metabolism, glutamate, Escherichia coli, cell tenacity

Judul: Penambahan Glutamat sebagai Pelindung Tekanan Osmosis dalam Kondisi Pembiakan Etanol Tinggi pada Rekombinan Escherichia coli
Abstrak: Enhancement of Escherichia coli recombinant cell growth in high ethanol culture conditions was investigated by additional glutamate as osmoprotectant. It was also supported by analysis of dry cell weight (DCW), glucose uptake, and acetate accumulation. Escherichia coli cells were grown under aerobic conditions with ethanol and glutamate addition in the culture time of t = 6 h. In high ethanol culture conditions (20 g/l ethanol) without glutamate acid additions, DCW and glucose uptake reached in 0.91±0.14 g/l and 13.23±0.89 g/l, respectively. These values were lower than culture conditions with 2 g/l glutamate acid additions (1.8±0.03 g/l DCW and 17.75±0.43 g/l glucose uptake), which also suppressed the accumulation of acetate in 3.29 g/l. Based on carbon flow in central carbon metabolism, the accumulation of acetate was suppressed by glutamate additions in the culture because the carbon flow diversion to tricarboxylic acid (TCA) cycle instead of entering the acetate. The accumulation of acetate also showed as a result of an overflow metabolism by high rate of glucose uptake and oxygenation level.
Keyword: Bogor Agricultural University (IPB), overflow metabolism, glutamate, Escherichia coli, central carbon metabolism, acetate

Judul: Warna bulu kucing ras Persia : variasi pola warna dan pewarisannya
Abstrak: Kucing Persia merupakan kucing ras yang paling umum dijumpai di Indonesia. Kucing Persia memiliki bulu yang panjang dengan variasi warna bulu yang sangat beragam. Perkawinan yang lebih terarah sangat perlu dilakukan untuk meningkatkan kualitas genetik warna dan pola warna bulu kucing. Penelitian ini bertujuan untuk mempelajari variasi warna dan pola warna bulu kucing Persia yang terdapat di Indonesia, yang dilakukan di kantor pusat Cat Fancy Indonesia mulai bulan Oktober 2000 sampai Januari 2001. Data yang digunakan merupakan data pedigree kucing Persia yang tercatat di Cat Fancy Indonesia dari tahun 1997 sampai Oktober 2001 yang terdiri dari 187 jantan dan 258 betina, selain itu juga dilakukan pengamatan langsung kepada tiga penyayang dan breeder kucing yang ada di Bandung. Secara umum tercatat 104 penyayang dan breeder kucing ras di Indonesia, 74,04% terdapat di Jawa Barat dan sisanya (25%) terdapat di daerah Jakarta. Jumlah kucing Persia yang lahir di Indonesia adalah 387 kucing (87,16%), yang terdiri dari 163 (42,12%) jantan dan 224 (57,88%) betina. Jumlah kucing Persia yang diimpor ke Indonesia adalah sebanyak 57 (12,845%) yang terdiri dari 23 (40,35%) jantan dan 34 (59,65%) betina. Jumlah kucing Persia yang terdapat di Jawa Barat (84,14%) lebih banyak dibanding jumlah kucing Persia yang ada di Jakarta (14,86%). Secara umum warna bulu cream (9,01%) dan pola warna solid (34,01%) memiliki persentase tertinggi. Warna cream merupakan warna dilusi dari warna merah (red) yang dapat dihasilkan dari berbagai macam kombinasi warna bulu yang dimiliki tetua sebelumnya, sebagai contoh dari perkawinan tetua yang memiliki warna bulu cream dengan red smoke. Perkawinan antara tetua yang memiliki warna dan pola warna bulu yang tidak murni dapat menghasilkan turunan yang memiliki warna dan pola warna bulu yang bervariasi.
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