Judul: Penelusuran karakteristik kelompok pelanggan churn: Studi kasus pelanggan churn PT Telkomsel Jakarta
Abstrak: Penelusuran kelompok pelanggan churn yang terdiri dari churn stop dan churn switch berdasarkan peubah yang diamati ditujukan untuk mengetahui karakteristik kelompok pelanggan churn. Persepsi yang selama ini ada mengatakan bahwa kedua kelompok tersebut adalah berbeda. Untuk membuktikan kebenaran dari persepsi tersebut maka dilakukan suatu perbandingan sekaligus pengujian secara statistik terhadap kedua kelompok tersebut berdasarkan peubah yang diamati. Pengamatan berdasarkan Proporsi responden dari setiap kategori peubah yang diamati menghasilkan kesimpulan bahwa kedua kelompok tersebut adalah sama. Begitu juga dengan hasil pengujian secara statistik yang dilakukan dengan menggunakan Uji Mann-Whitney dan Uji Kehomogenan. Pengujian ini menghasilkan kesimpulan bahwa kelompok pelanggan churn stop dan kelompok pelanggan churn switch berdasarkan peubah yang diamati tidak ada perbedaan antara satu sama lain. Dengan kata lain, kita belum bisa menyatakan bahwa kedua kelompok pelanggan churn stop dan churn switch adalah berbeda. Hal ini tidak mendukung persepsi yang selama ini ada bahwa kedua kelompok pelanggan churn berbeda.
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Judul: Understanding Customer Insight by Segmenting, Profiling, and Churn Analysis in Mobile Telecommunication
Abstrak: As the telecommunication industry has rapidly developed, it may cause tighter competition among providers. Accurate customer analysis such segmentation, profiling, and churn analysis are the appropriate tools to encounter this challenge. Providers compete fiercely to each other in acquiring new subscribers and retaining the existing ones to raise profitability. To gain high value customer, providers need to know well about their customers; what they wants and what they needs. So that it is necessary to classify subscribers in several segments and profile them according to desirable criteria. Moreover, provider also has to recognize the predominant factors influence customer churn. Conventional statistical methods such Logistic Regression is very successful in predicting customer into churner or non-churner. Furthermore it is needed to accommodate changes of customer characteristics by forecasting the time of ‘churner’ predicted churn using survival analysis. It is possible to diagnose and repair the root causes of customer resignation and dissatisfaction based on logistic regression and survival analysis.
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Judul: Gene Editing  of Two Cyanide Synthesis-related Genes in Cassava using CRISPR/Cas9
Abstrak: Cassava (Manihot esculenta) contain toxic quantities of cyanogenic glucoside. Reducing the potentially toxic cyanogenic glucoside levels in cassava could be achieved by gene editing through Clustered Regularly Interspaced Short Palindromic Repeats – CRISPR associated protein 9 (CRISPR/Cas9), a high- precision genome editing technique in plants. Two cassava UDP- glucosyltransferase (UGT) paralogs, UGT85K4 and UGT85K5, are the genes catalyzing the synthesis of cyanogenic glucosides in plants. The study aimed to construct the CRISPR/Cas9 recombinant vector carrying single guide RNA (sgRNA) to edit UGT85K4 and UGT85K5 genes, and transform sgRNA CRISPR/Cas9 construct into KU50 cassava calli. Five sgRNAs were designed, considering the on/off target efficiency score and GC content. Those sgRNAs were ligated into pRGEB32 plasmid and introduced to competent Escherichia coli DH5a. The PCR colony and DNA sequencing were performed to verify the sgRNA insert. The amplicons showed the expected band size of recombinant vector (180 bp). Based on sequencing results, only one sgRNA was successfully ligated into the plasmid that will be used to edit the UGT85K5 gene. The sgRNA CRISPR/Cas9 construct in pRGEB32 plasmid was successfully transformed into Agrobacterium tumefaciens and introduced into KU50 cassava calli through co-cultivation. The overgrowth of Agrobacterium occurred after the co-cultivation phase.
Keyword: CRISPR/Cas9, Cyanogenic glucoside, Gene editing, sgRNA