Judul: Analisis Respon Toleransi Padi Nipponbare Transgenik Terhadap Salinitas Tinggi
Abstrak: The global climate change causing concerns and challenges ahead in improving the productivity of rice continuously. Increasing the temperature of the earth causes increased salinity on land around the beach due to rising sea levels. Anticipatory measures and strategically to deal with these changes is improve the nature of rice tolerant to high salinity through genetic engineering approaches. OsDREB1A and OsERF1 is a specific transcription factor genes of plants that a role in inducing other genes that responsible regulate response to stress salinity. This study aims to analyze response tolerance transgenic rice Nipponbare- OsDREB1A and Nipponbare-OsERF1 to high salinity. Analysis carried out by polymerase chain techniques reaction (PCR) using primers HPT and for selection used NaCl concentration 25 mM and 150 mM. Results treatment of 25 mM NaCl stress obtained 38 plants positive transgenic gene contains factors OsDREB1A and OsERF1 transcription of 48 plants putative transgenic with high growth characteristics of plants and root length was better than the wild type. Results selection of 150 mM NaCl stress obtained 23 plants positive transgenic of 29 plants putative transgenic with characteristics can lead a normal life when all types of wild rice dead.
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Judul: Over-expression of OsDREB1A gene for improvement of drought tolerance on rice.
Abstrak: Drought is a major factor that influence the growth and productivity of rice. Drought tolerant rice varieties are needed to increase rice production. Development of improved rice varieties can be done through classical breeding and genetic transformation. The research objective was to obtain rice plants containing the construct 35S::OsDREB1A. Agrobacterium tumefaciens mediated transformation of rice cv. Nipponbare using OsDREB1A gene under the control of CAMV 35 promoter produced 81 putative transgenic rice plants (T0) positively containing the transgene based on PCR analysis. The plant height, panicle number, panicle length, and the grain number of transgenic plants were in general lower than the Nipponbare wild type. T1 seeds from selected genotypes were screened for hygromycine resistence, and rice seedlings were evaluated for tolerance to drought stress in the glasshouse, based on the standard evaluation system for rice (IRRI 1996). Five independent transgenic rice plants were identified to be tolerant to drought stress. Molecular characterization using Southern hybridization showed that these plants had 2- 4 copies of transgene. Physiological analysis showed that the drought avoidance is the possible mechanism of adaptation to drought of the transgenic plants. The drought tolerant transgenic plants were crossed to Indonesian elite rice cultivars (Ciherang, Batutegi, Code, and Konawe). Glasshouse evaluation showed that F1 transgenic plants derived from cultivars Batutegi, Code, and Konawe were more tolerant to drought than their wild-type counterparts. F1 transgenic plants were backcrossed into corresponding elit rice cultivars, and PCR analysis showed that those plants contained the transgene. These results showed that 35S::OsDREB1A could be transferred and inherited to elite rice genotipes through crossing. The new drought tolerant germplasms generated in this research can be useful materials for breeding program for improvement of drought tolerance on rice in Indonesia.
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Judul: Cycle Hamilton pada Graf Lengkap, Graf Regular, dan Graf 2-Connected 4-Regular Berorder Kurang dari Sepuluh
Abstrak: Suatu graf disebut graf Hamilton apabila graf tersebut memuat cycle Hamilton, yaitu cycle yang melewati setiap vertex tepat satu kali. Menentukan keberadaan cycle Hamilton pada suatu graf dapat menggunakan syarat cukup yang berupa teorema-teorema yang telah diperkenalkan sejak tahun 1950-an. Beberapa syarat cukup yang dibahas dalam karya ilmiah ini adalah syarat cukup yang berhubungan dengan graf lengkap dan graf regular. Suatu graf lengkap berorder 𝑛������ ≥ 3 dan graf r-regular berorder 𝑛������ ≤ 2𝑟������ + 1 merupakan graf Hamilton, Karena kedua graf tersebut memuat cycle Hamilton. Selanjutnya, syarat cukup tersebut digunakan untuk menentukan keberadaan cycle Hamilton pada graf 2-connected 4- regular berorder kurang dari 10.
Keyword: graf Hamilton, graf regular, graf lengkap, graf 2-connected 4-regular