Judul: Pendeteksian Penutupan Lahan Menggunakan Citra Landsat-TM dengan Metode OIF dan PCA (Studi Kasus : Pesisir Teluk Banten, Jawa Barat)
Abstrak: Wilayah pesisir dan lautan merupakan ekosistem yang bemilai tinggi di dunia, dimana pesisir menyusun sebuah zona penyeimbang (buffer zone) antara lant dengan daratan dengan tingkat prodnktivitas yang tinggi (Nybakken, 1998). Didalam pesisir terdnpat satu atan lebih sistem lingkungan (ekosistem) pesisir dan sumberdaya pesisir. Ekosistem tersebut dapat bersifat alamiah ataupun buatan (man-made).
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Judul: Pemantauan Garis Pantai dengan Menggunakan Citra Landsat-TM. Studi Kasus di Daerah Muara Sungai Cisadane
Abstrak: Penanganan dan pemanfaatan perairan pesisir di Indonesia, tidak terlepas dari pengumpulan data mengenai lingkungan perairan pesisir itu sendiri. Tersedianya data yang memadai dan baik merupakan salah satu faktor keberhasilan dari penanganan dan pemanfaatan perairan pesisir.
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Judul: Excretory/secretory antigen of ascaridia galli L3 stage to trigger yolk immunoglobulin in laying hens
Abstrak: Ascaridiosis caused by the nematode Ascaridia galli. A study was carried out to characterize excretory/secretory antigen of A. galli from larval stage to trigger anti-ascaridiosis yolk immunoglobulin in laying hens. A. galli L3 were recovered from intestines of 100 heads chickens 7 days after oesophagus inoculation with 6000 L2. L3 recovered in this manner were cultured (5 – 10 ml-1) in flasks containing rosswell park memorial institute (RPMI) 1640 media, pH 6.8, without phenol red. Cultures were incubated at 370C in 5% CO2 and culture fluid was collected after 3 days in culture. To prepare excretory/secretory antigen, culture medium were concentrated with vivaspin 30.000 MWCO, and antigen protein concentration were counted as described in Bradford method. The molecular weight of excretory/secretory antigen was determined with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). Active immunizations were applied intra muscularly of chickens with an initial dose of 80 μg. The immunizations were repeated three times with dose of each 60 μg with an interval of one week. The first immunizations were excretory/secretory antigen mixed with fruend adjuvant complete and subsequently mixed with freund adjuvant incomplete. The chicken’s eggs were collected from 49 day after immunizations. Anti-ascaridiosis yolk immunoglobulin (IgY) was extracted from egg yolks by means of ammonium sulphate and purified using fast performance liquid chromatography (FPLC). The purity of anti-ascaridiosis IgY was determined by Bradford method (λ = 280 nm). Antibody was detected by agar gel precipitation test (AGPT) and enzyme linked immunosorbant assay (ELISA). Antigen were detected with immunohistochemistry. Chickens were challanged with dose of 1000 L2 A. galli. Passive immunizations were applied ten times with dose of each 0,875 mg IgY with an interval of one day intra orally. IgY anti-ascaridiosis was tested against A. galli survival in vitro.
Keyword: Unggas, Isolasi dan karakterisasi antigen, Purifikasi imunoglobulin yolk, Pengukuran antibodi ayam petelur, Metode Imunohistokimia, Ascaridiosis