Judul: Kloning gen CYP71AV asal Artemisia annua di dalam Escherichia coli DH-5α
Abstrak: Infection of malaria caused 1.7-2.5 million people die each year. Artemisia annua contains artemisinin compounds effective to eradicate malaria types that are resistant to quinine and chloroquine. CYP71AV is a gene that has critical role in the biosynthesis of artemisinin by catalyzing the oxidation of intermediate products Amorpha-4,11-diene, which are highly produced in the biosynthesis of artemisinin. This study aimed to clone the CYP71AV gene through recombinant DNA technology. CYP71AV gene from total cDNA was amplified using specific primers. CYP71AV gene was inserted into pGEM-T Easy plasmid. The recombinant plasmid containing CYP71AV gene introduced into Escherichia coli strain DH5-α. CYP71AV gene sequencing produce approximately 1571 base pairs encoding 523 amino acids. Local alignment analysis of nucleotides with CYP71AV of several species listed in GeneBank showed that the cDNA CYP71AV have 98% similarity with complete cds A.annua (JQ254992.1). Domain analysis showed that the CYP71AV gene has three conserved domain
Keyword: Bogor Agricultural University (IPB), Artemisia annua., clone, cDNA CYP71AV

Judul: Identifikasi Populasi Mutan Artemisia annua L. Hasil Induksi Sistem CRISPR.
Abstrak: Artemisia annua L. (A annua) adalah tanaman yang mengandung artemisinin, senyawa yang efektif mengobati malaria. Walaupun demikian, kandungan artemisinin sangat kecil pada A annua. Dalam rangka meningkatkan kandungan artemisinin, dilakukan rekayasa jalur metabolik dengan sistem clustered regularly interspaced short palindromic repeats (CRISPR). Tujuan penelitian ini adalah mengidentifikasi tanaman populasi A annua yang mengalami mutasi setelah diinduksi oleh sistem CRISPR. Penelitian ini dimulai dengan preparasi benih, sterilisasi benih, isolasi DNA tanaman, optimasi suhu annealing primer, amplifikasi DNA tanaman, dan analisis dengan elektroforesis. Identifikasi yang dilakukan menunjukkan bahwa terdapat 46 tanaman yang berhasil diamplifikasi dan sebanyak 24 tanaman lainnya tidak berhasil diamplifikasi. Ukuran amplikon artemisia adalah berkisar 700 bp sedangkan ukuran amplikon plasmid yang merupakan kontrol positif hanya berkisar 500 bp. Sebanyak 46 sampel DNA kembali diamplifikasi dan dianalisis dengan elektroforesis QIAxcel. Sebanyak 39 sampel ditemukan mengalami mutasi. Ukuran basa nukleotida bervariasi yaitu 465 bp, 466 bp, 467 bp, dan 468 bp sedangkan ukuran plasmid adalah 468 bp.
Keyword: Artemisia annua L., artemisinin, CRISPR/Cas9

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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