Judul: Produksi Oligosakarida dari Umbi Kentang Hitam melalui Hidrolisis Ekstrak Kasar α-Amilase asal Brevibacterium sp.
Abstrak: Oligosaccharides is a carbohydrate macromolecule composed of 2-10 monosaccharide residues that have benefits in the field of food and health. One way to produce oligosaccharides with enzymatic hydrolisis of starch. Black potatoes have high carbohydrate content, especially starch by 83%. Activity of amylase enzyme from raw extraction have 1.708 U/ml. Degree of polymerization of black potatoe’s starch is 10. Hydrolysis reaction of black potatoe’s starch with that enzyme gave 3 spots on TLC analysis with mobile phase n-buthanol:acetic acid:water purified (2:1:1). In HPLC analysis showed 3 main peaks that means consist of 3 compound with mobile phase acetonitrile:water (75:25). Fresh sample consist of glucose as many as 1,60% (b/v), maltose 0,36% (b/v), and maltotriose 0,46% (b/v). Freeze dried sample consist of glucose as many as 4,20% (b/v), maltose 0,21% (b/v), and maltotriose 0,30 % (b/v).
Keyword: Brevibacterium sp., α-amylase enzyme, HPLC, oligosaccharides, black potatoes

Judul: Analisis oligosakarida hasil hidrolisis pati kentang hitam (Culeus tuberosus) oleh Enzim amilase dari Brevibacterium sp.
Abstrak: Oligosaccharide is an important group of carbohydrate macromolecul composed of 2 to 20 saccharide units. Oligosaccharide has many benefits in the field of food and health. Starch is one of the source origin material for oligosaccharide production by enzimatic process. Black potatoes contain high carbohydrates especially starch of about 83%. To obtain oligosaccharide from black potato starch can be hidrolyzed using amylase enzymes which are produced by Brevibacterium sp. Crude extracts amylase enzyme production from Brevibacterium sp. producing enzyme activity of 3.25 U/mL. Black potato starch hydrolysis results a value of reducing sugar of 7482.500 ppm, after freeze drying a value of reducing sugar was 9647.500 ppm, and after concentrated by vacuum evaporator the value of reducing sugar was 14908.300 ppm. The results of HPLC analysis of black potato starch hydrolysis showed that there are oligosaccharide compound such as maltose, maltotriose, and maltopentose. The samples were given preferential treatment freeze dry and evaporation produce reducing sugar value and concentration of the oligosaccharide better than the fresh samples without treatment. The identification result of oligosaccharide obtained by HPLC shows that freeze drying and evaporating samples has a higher concentration and better peak and sharp.
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Judul: Etno-ornitologi Burung Kancilan Flores (Pachycephala nudigula nudigula Hartert 1897) pada Masyarakat Lio di Taman Nasional Kelimutu
Abstrak: Bare-throathed whistler is one of several wild birds in Kelimutu National Park (TNKL) that is believed to have correlation with Lio Community‟s belief. The estimation of Bare-throated whistler‟s population in Kelimutu National Park was about 186.41-1668.07 individual by the density about 0.0389-0.3481 ind/ha with the sex ratio 6.2:1 that spreaded on area with height of 1100 – 1600 masl. The characteristic of Bare-throated whistler‟s habitat is a natural forest with various canopy level, high plant species diversity and high density. The habitat characteristics related to the availability of food in the form of insects, cover, safety and a nesting site. Generally the most of Lio community knew the existence of Bare-throated whistler directly or from their parents‟ stories. The perceptions of Lio Community to the Bare-throated whistler‟s existence is generally support the conservation efforts. The perceptions construct the Lio Community‟s atitude that tend to be conservative to the existence of Bare-throated whistler. The attitude shown by their behavior that not cathing the Bare-throated whistler from the TNKL‟s area or the outside. The attitude have an important role to the existence of Bare-throated whistler in Kelimutu National Park‟s area.
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