Judul: Efektivitas Imunoglobulin Y (IgY) Antilipase sebagai Inhibitor Enzim Lipase Pankreas untuk Pencegahan Obesitas
Abstrak: Obesity has been a major risk for many degenerative diseases with an increasing prevalence.Pancreatic lipase is responsible for 50-70% of total dietary fat absorbtion and has been studied widely for new anti-obesity invention. Hen immunized with porcine pancreatic lipase will produce yolk containing IgY antilipase. IgY antilipase will bind the active site of pancreatic lipase in gastrointestinal tract and inhibit the activity of pancreatic lipase in the gastrointestinal tract. This research was conducted to evaluate the effectivity of IgY antilipase as anti-obesity. IgY antilipase was purified with sodium sulphate precipitation. The protein was analysed by SDS-PAGE and was identified by Immunoblotting. The ability of IgY antilipase to inhibit pancreatic lipase was tested by colorimetric microplate assay at five grades of concentration. Yolk containing IgY antilipase was then tested in rabbit to evaluate its ability to inhibit fat absorbtion in vivo. The result showed that IgY contained two proteins with molecular weights of 61.2 kDa and 26.9 kDa. IgY was confirmed because it positively reacted with rabbit-anti-chicken HRP. The average IgY concentration was 30.1 ± 6.3 mg/mL yolk. IgY antilipase and orlistat did not differ in inhibition capacity at various concentrations (p<0.05). The inhibition capacity of IgY obtained from unimmunized hen was significantly lower as compared to those of IgY antilipase and orlistat (p<0.05). Yolk containing IgY antilipase also had the ability to inhibit dietary fat absorbtion in vivo, but its inhibition capacity was lower than that of orlistat (p<0.05). It is concluded that IgY antilipase could be used to inhibit fat absorbtion to prevent obesity.
Keyword: IgY antilipase, orlistat, anti-obesity, pancreatic lipase inhibition, diatery fat absorbtion

Judul: Uji Potensi Inhibisi Ekstrak Daun Sirih Merah terhadap Lipase Pankreas sebagai Antiobesitas secara In Silico
Abstrak: Obesitas menjadi pemicu munculnya berbagai penyakit degeneratif, seperti stroke, jantung, diabetes tipe 2, dan resistensi insulin. Inhibitor lipase pankreas merupakan agen obat antiobesitas dengan kerja menginhibisi enzim lipase pankreas. Penelitian ini bertujuan melakukan pencarian dan prediksi aktivitas inhibisi terhadap lipase pankreas dari senyawa aktif daun sirih merah. Penelitian dimulai dengan penapisan virtual 60 ligan uji, prediksi stabilitas dan toksisitas ligan, validasi penambatan molekuler, penambatan molekuler, dan visualisasi 2D serta 3D. Hasil menunjukkan bahwa benzethonium octyloxyacetate, hydroxybenzoic acid β-D-glucosyl ester, dan anthocyanins memiliki potensi terbaik dalam menginhibisi enzim lipase pankreas berdasarkan penapisan virtual, stabilitas ligan, toksisitas ligan, energi afinitas, jumlah ikatan hidrogen dengan sisi aktif, jarak ikatan, dan konstanta inhibisi (Ki). Benzethonium octyloxyacetate memiliki nilai afinitas sebesar -8 kkal/mol, memiliki interaksi ikatan hidrogen pada residu His349 sisi aktif reseptor lipase pankreas (6KSM) dengan jarak ikatan 2,94 Å, dan nilai Ki sebesar 1,347 μM., Obesity triggers the emergence of various degenerative diseases, such as stroke, heart disease, type 2 diabetes, and insulin resistance. Pancreatic lipase inhibitors are anti-obesity drug agents that work by inhibiting pancreatic lipase enzymes. This study aims to search and predict the inhibitory activity of pancreatic lipase from the active compound of red betel leaf. The research began with virtual screening of 60 test ligands, prediction of ligand stability and toxicity, validation method of molecular docking, molecular docking, 2D and 3D visualization. The results showed that benzethonium octyloxyacetate, hydroxybenzoic acid β-D-glucosyl ester, and anthocyanins had the best potential in inhibiting pancreatic lipase enzymes based on virtual screening, ligand stability, ligand toxicity, energy affinity, number of hydrogen bonds with active site, bond distance, and constants inhibition (Ki). Benzethonium octyloxyacetate has an affinity value of -8 kcal/mol, has a hydrogen bond interaction on the His349 residue on the active site of the pancreatic lipase receptor (6KSM) with a bond distance of 2,94 Å and Ki value of 1,347 μM.
Keyword: molecular docking, pancreatic lipase, obesity, red betel

Judul: Morfologi Tanaman dan Fenologi Pembungaan Tacca chantrieri Andre
Abstrak: Tacca chantrieri Andre, salah satu spesies herba tropis keluarga Taccaceae memiliki nilai estetika yang tinggi sebagai komoditas tanaman hias. Bunga Tacca mempunyai bentuk yang unik, umumnya berwarna ungu, mempunyai aksesori menyerupai kumis yang panjang dan banyak, dengan braktea lebar seperti sayap kelelawar. Informasi mengenai Tacca masih sangat terbatas dan tanaman ini mulai sulit ditemukan di habitatnya. Penelitian ini bertujuan mendapatkan informasi morfologi tanaman dan pertumbuhannya, fenologi pembungaan serta perkembangan benih. Penelitian dilaksanakan di Laboratorium Mikro Teknik dan Laboratorium Biologi dan Biofisik Benih, Departemen Agronomi dan Hortikultura, Laboratorium Biosistematika Serangga, Departemen Proteksi Tanaman, dan screen house Anggrek-Lele (Angle), Kebun Percobaan Leuwikopo, Institut Pertanian Bogor sejak Februari-Desember 2016. Penelitian menggunakan 2 aksesi, yaitu aksesi Kalimantan, Indonesia dan Queensland, Australia. Pengamatan terhadap morfologi tanaman menunjukkan bahwa aksesi Kalimantan mempunyai daun berwarna hijau dengan posisi tangkai yang berserak, sementara aksesi Queensland mempunyai daun berwarna kecoklatan dengan posisi tangkai tegak. Struktur eksternal benih aksesi Kalimantan memiliki bentuk seperti ginjal berwarna coklat gelap dan membulat sedangkan aksesi Queensland berwarna emas kecoklatan dan berbentuk lebih ramping memanjang. Aksesi Kalimantan mulai berbunga pada saat jumlah daun mencapai empat daun dewasa, dengan diameter batang 3-10 cm dan terus berbunga setiap bulan, sementara aksesi Queensland belum berbunga sama sekali, walaupun rata-rata jumlah daun telah mencapai 8 daun dewasa dan diameter batang 5-12 cm.
Keyword: Bunga kelelawar, karakter botani, pembungaan, struktur bunga, aksesi