Judul: Transformasi Genetik Kentang (Solanum tuberosum L.) Kultivar Baraka dengan Gen Pembungaan Hd3a
Abstrak: Flowering has a very important role in plant breeding. Flowering is the phase transition from the vegetative to the generative phase. One of the genes associated with flowering is Hd3a. This research has an objective to transform genetically potato (Solanum tuberosum L.) cultivar Baraka with Hd3a flowering gene under control of a strong promoter prolC. Potato plants were propagated in vitro on MS2 medium. Genetic transformation was carried out by co-cultivation method by using Agrobacterium tumefaciens LBA4404. Leaf disc and internodes were used as explants for transformation. Selection of transgenic plants was performed in MS medium containing 10-40 mg/L hygromycin. The results showed that the efficiency of transformation was relatively low. The efficiency of shoot regeneration of transgenic calli was very high. The low efficiency of transformation was caused by damage of explant during transformation process. The efficiency of transformation of internode was higher than leaf explants. Putative transgenic shoot regeneration per callus was 2.5. In MS0 media, four of twenty-four putative transgenic plants produced tuber whereas all non-transgenic plants did not. This result showed that Hd3a induced the formation of tuber of transgenic potato cv. Baraka.
Keyword: Bogor Agricultural University (IPB), Hd3a flowering gene, genetic transformation, Solanum tuberosum L. cultivar Baraka

Judul: Rekayasa genetik pada tanaman kentang (Solanum tuberosum) kultivar Diamant dengan gen Hd3a dibawah kendali promoter 35SCaMV
Abstrak: Potato is one of important horticultural and food crops in Indonesia due to the high economic value. Flower has an important role in the conventional breeding to cross, so the induction of flowering is very important. In several species, Hd3a induced flowering. In potato cv. Andigena, Hd3a does not only induce the flowering, but also induces the tuber formation. This research has an objective to engineer genetically potato cv. Diamant with Hd3a under the control of the 35SCaMV promoter. Potato plants were propagated in vitro in MS2 macro media. Genetic transformation is done by using the internode fragments as explants intermediated by Agrobacterium tumefaciens with co-cultivation method. Calli and putative transgenic plants sere selected in MS media containing 10-40 mg/l hygromicin. The results showed that the average transformation efficiency was 38.88% with low regeneration efficiency, i.e. 9.5%. The average of shoots produced per callus was 2.25. At six weeks old, putative transgenic and non transgenic potato plants did not form a flower, but 10% of putative transgenic 3 plants formed a tuber and all non transgenic ones did not produce a tuber. This result indicates that Hd3a is capable to induce tuber formation. Six weeks is not enough time for flower formation.
Keyword: Bogor Agricultural University (IPB), Solanum tuberosum, Hd3a gene, genetic transformation, cv. Diamant, 35SCaMV

Judul: Asymptotic Distribution of Estimator for the Intensity Function of a Doubly Periodic Poisson Process
Abstrak: In this manuscript, estimation of the intensity function of a doubly periodic Poisson process is discussed. It is assumed that only a single realization of the Poisson process is observed in a bounded window [0,n]. It is also assumed that the period of the intensity function is known. The rate of consistency and asymptotic normality of the estimator are formulated. Finally, a confidence interval for the local intensity function is also formulated.
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