Judul: Respon Fisiologis dan Tingkah Laku Domba Ekor Tipis dengan Waktu Pemberian Pakan yang Berbeda
Abstrak: This study aimed to examine the relationship of feeding time on physiological response and the effect of feeding time on behavior of Javanesse thin tailed sheep. The composition of feed was 70% of concentrate + 30% of grass. The treatment in this study is the feeding time in the morning and evening. Parameters of physiological response that had been observed were heart rate, respiration rate, rectal temperature. Whilethe observed behavior were locomotive, ingestive and resting. This research was designed by completely randomized design with 2 treatments of feeding time with 6 replications for each of it. Data processed by ANOVA. The behavior data was analyzed by student’s test. The result showed that physiological responses of Javanese Thin Tailed Sheep were normal. Locomotive behavior of sheeps by feeding treatment in the morning was higher in the daytime. Ingestive behavior of sheeps by feeding treatment in the morning and afternoon completely reversed. Resting behavior was more frequently in the night in both paramaters.
Keyword: behavior, physiological responses, Javanese thin tailed sheep

Judul: Respon Fisiologis dan Tingkah Laku Domba Garut dengan Pakan Limbah Tauge atau Kangkung Kering sebagai Pengganti Rumput
Abstrak: Garut sheep is commonly reared in West Java and usually fed with grass as the main feed and energy source. However, this grass has low quality and seasonally available, therefore should be an alternative feed such as mung bean sprout waste and dried kangkung. This research aimed to study sheep physiology and behavior as a response to diet and environment. This research used nine garut sheep (I0) with three treatments and replicates. The treatments were P0 (50% grass+50% consentrate), P1 (50% mung bean sprout waste+50% concentrate) and P2 (50% dried kangkung+50% concentrate). Physiological response data were analyzed by analysis of variance (ANOVA) and tested by Tukey post test method, while behavior frequency data were analyzed by Kruskal-Wallis test. Result showed that diet treatment did not affect (P>0.05) the heart and respiration rate, but significantly affected (P<0.05) on rectal temperature. All behaviors parameters were not significantly different (P>0.05) except for resting, grooming, licking strange object, and locomotion were higher in P1 than P0 and P2.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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