Judul: Identification of polymorphism of the Growth Hormone Gene and its Receptor and their Association with Partial Cumulative Milk Yield in Holstein Friesian Cattle in West Java Production Center
Abstrak: Growth hormone gene has a critical role in the regulation of lactation, mammary gland development and growth process through its interaction with its specific receptor. Growth Hormone (GH) and Growth Hormone Receptor (GHR) genes have been suggested as candidate genes for traits related to milk production. The purpose of this study was to identify genetic polymorphism of the Growth Hormone (GH) and the Growth Hormone Receptor (GHR) genes, and the association of their genetic variants with milk yield in Holstein Friesian (HF) cattle. A total of 370 HF cattle from six populations of Institute for breeding of dairy cattle Cikole station (88 samples), Pasir Kemis (96 samples), Cilumber (98 samples), Lembang Artificial Insemination Center (LAIC) (17 samples), Livestock Embryo Transfer Center (40 samples) and Singosari Artificial Insemination Center (SAIC) (32 samples) were genotyped by the Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP) methods. Genotyping the GH gene with Alu1 restriction enzyme to all DNA samples produced two variant genotypes, i.e LL (89 %) and LV (11%) genotypes. The frequency of L allele was 94 % and V allele was 6%. Genotyping of GHR gene with Alu1 restriction enzymes produced three genotypes and two alleles. The frequencies of AA, AG, and GG genotypes were 58 %, 34%, and 8% respectively. The frequency of A allele was 75% and G allel was 25%. There was no association between GH and GHR polymorphism with milk production.
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Judul: Identifikasi keragaman Gen Growth Hormone Receptor (GHR AluI) pada Sapi Friesian Holstein di BIB Lembang, BBIB Singosari, dan BET Cipelang.
Abstrak: Growth hormone receptor (GHR) is one factor affecting animal growth. GHR is required by growth hormone (GH) to carry out its effect on target tissues. The objective of this study was to identify polymorphism of the Growth Hormone Receptor gene in Holstein Friesian (HF) dairy cattle and beef cattle. Genotyping was performed on 126 animals. These consisted of HF 89 heads from BIB Lembang (17), BBIB Singosari (32), and BET Cipelang (40); as well as for 37 heads of four breeds of beef cattle including for Simmental (13), Limousin (14), Brahman (5), and Angus (5) for a comparison. Varian genetic of the GHR gene was identified by PCR-RFLP method using AluI restriction enzyme cutting the base position at AG|CT. Single nucleotide polymorphism (SNP) had been found in exon 10, coding for the cytoplasmic domain of GHR, which was located at position 81 bp (A/G) induced amino acid substitutions Ser/Gly. The product of amplification of GHR was 298 bp. Genotyping the GHR gene produced two alleles (A and G) resulting three genotypes (AA, AG and GG). The genotype AA was dominant in HF cattle from all locations, and also in Brahman and Limousin cattle from BET Cipelang. All of HF dairy cattle were in Hardy-Weinberg disequilibrium (χhit 2>χ0,05 2), in contrast, Simmental and Limousin were in Hardy-Weinberg equilibrium. The Hardy-Weinberg in Brahman and Angus values could not be calculated. Heterozigosity values in HF and beef cattle were low (ho<he). In beef cattle, Angus had highest heterozigosity value (0,600), while Brahman had the lowest heterozigosity value (0.000). The conclusion was that the variability of GHR|AluI gene can be used as a basic information for selection in growth caracteristic in the HF and beef cattle. Keywords: Beef cattle, Genetic polymorphism, GHR|AluI gene,
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Judul: Penerbangan Otomatis Pesawat Tanpa Awak Jenis Sayap Tetap dengan iNav
Abstrak: Pesawat tanpa awak (UAV) dapat dikendalikan secara manual dengan remote control atau secara otomatis dengan flight controller (FC). Salah satu jenis UAV adalah sayap tetap yang memiliki bentuk seperti pesawat terbang pada umumnya. Penelitian ini bertujuan untuk melakukan penerbangan otomatis pada UAV jenis sayap tetap dengan mengkonfigurasi perangkat FC. UAV yang digunakan adalah model SkySurfer X8 dengan bentang sayap 1.4 m. FC yang digunakan adalah Matek F405-Wing yang dikonfigurasi dengan perangkat lunak iNav. Berdasarkan hasil uji terbang, UAV berhasil terbang secara otomatis mengikuti skenario yang diberikan. Skenario terjauh berupa persegi berukuran 600×600 m pada ketinggian 100 m, yang ditempuh dalam waktu sekitar 2 menit dengan kecepatan sekitar 65 km/jam. Diharapkan prosedur perakitan, konfigurasi, operasi, dan hasil data uji terbang dapat menjadi acuan dalam penelitian selanjutnya.
Keyword: iNav, penerbangan otomatis, pesawat tanpa awak, sayap tetap