Judul: Identifikasi Keragaman Gen Growth Hormone Receptor Exon 8 (GHR|SspI) pada Sapi Friesian Holstein dengan Metode PCR-RFLP
Abstrak: Growth Hormone Receptor (GHR) is one factor affecting animal growth. GHR is required by growth hormone (GH) to carry out its effect on target tissues. The objective of this study was to identify polymorphism of the Growth Hormone Receptor gene in dairy cattle (Holstein Friesian) from BPPT SP Cikole, KPSBU Pasir Kemis, KPSBU Cilumber, BIB Singosari and BBIB Lembang. The gene polymorphism was identified with Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method using SSpI restriction enzyme. Genotyping in this study was performed on 312 animals. The product of amplification was 230 bp. Genotyping the GHR gene produced two alleles (A and T) and three genotypes (AA, AT, TT). Results from the analysis of the allele and genotype frequencies showed that the T allele had a higher frequencies than the A allele and TT genotype had a higher frequencies than AA or AT genotypes from all population. The GHR gene in HF from BPPT SP Cikole, KPSBU Cilumber, and KPSBU Pasir Kemis were in Hardy-Weinberg equilibrium. The equilibrium Hardy-Weinberg in HF from BIB Lembang and BBIB Singosari could not be calculated. Heterozigosity value of GHR gene (Ho) were lower than heterozigosity value in HF (He) from BPPT SP Cikole and KPSBU Pasir Kemis. In HF from other population, heterozigosity value of GHR gene (Ho) were higher than heterozigosity value (He). The conclusion was that GHR|SSpI gene in HF from BPPT SP Cikole, KPSBU Pasir Kemis, KPSBU Cilumber, BIB Lembang and BBIB Singosari was polymorphic.
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Judul: Identifikasi keragaman Gen Growth Hormone Receptor (GHR AluI) pada Sapi Friesian Holstein di BIB Lembang, BBIB Singosari, dan BET Cipelang.
Abstrak: Growth hormone receptor (GHR) is one factor affecting animal growth. GHR is required by growth hormone (GH) to carry out its effect on target tissues. The objective of this study was to identify polymorphism of the Growth Hormone Receptor gene in Holstein Friesian (HF) dairy cattle and beef cattle. Genotyping was performed on 126 animals. These consisted of HF 89 heads from BIB Lembang (17), BBIB Singosari (32), and BET Cipelang (40); as well as for 37 heads of four breeds of beef cattle including for Simmental (13), Limousin (14), Brahman (5), and Angus (5) for a comparison. Varian genetic of the GHR gene was identified by PCR-RFLP method using AluI restriction enzyme cutting the base position at AG|CT. Single nucleotide polymorphism (SNP) had been found in exon 10, coding for the cytoplasmic domain of GHR, which was located at position 81 bp (A/G) induced amino acid substitutions Ser/Gly. The product of amplification of GHR was 298 bp. Genotyping the GHR gene produced two alleles (A and G) resulting three genotypes (AA, AG and GG). The genotype AA was dominant in HF cattle from all locations, and also in Brahman and Limousin cattle from BET Cipelang. All of HF dairy cattle were in Hardy-Weinberg disequilibrium (χhit 2>χ0,05 2), in contrast, Simmental and Limousin were in Hardy-Weinberg equilibrium. The Hardy-Weinberg in Brahman and Angus values could not be calculated. Heterozigosity values in HF and beef cattle were low (ho<he). In beef cattle, Angus had highest heterozigosity value (0,600), while Brahman had the lowest heterozigosity value (0.000). The conclusion was that the variability of GHR|AluI gene can be used as a basic information for selection in growth caracteristic in the HF and beef cattle. Keywords: Beef cattle, Genetic polymorphism, GHR|AluI gene,
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Judul: Perencanaan Desain Embung di Sentul City.
Abstrak: Salah satu bangunan air yang dapat digunakan untuk menampung air hujan dan limpasan permukaan adalah embung. Penelitian ini bertujuan untuk mendapatkan desain embung di beberapa lokasi yang potensial di Sentul City. Dari 5 lokasi calon embung dipilih 3 lokasi yang sesuai persyaratan teknis untuk dijadikan embung. Lokasi 1 terletak pada koordinat 106.857° BT dan 6.532° LS, lokasi 2 terletak pada koordinat 106.850° BT dan 6.523° LS dan lokasi 3 terletak pada koordinat 106.849° BT dan 6.516° LS. Desain embung lokasi 1 memiliki ukuran lebar atas 57 m, panjang atas 65 m, lebar bawah 45 m, panjang bawah 53 m dan kedalaman 4 m. Desain embung lokasi 2 dan 3 memiliki ukuran lebar atas 100 m, panjang atas 80 m, lebar bawah 88 m, panjang bawah 68 m dan kedalaman 4 m. Kapasitas total embung lokasi 1, lokasi 2 dan lokasi 3 saat terisi penuh masingmasing sebesar 12 083.48 m3, 27 870.61 m3 dan 27 870.61 m3. Biaya pembuatan satu unit embung di lokasi 1, lokasi 2 dan lokasi 3 berturut-turut sekitar Rp 743.4 juta, Rp 1.8240 miliar dan Rp 1.8763 miliar.
Keyword: biaya pembangunan, desain, embung, limpasan permukaan