Judul: Spatial Analysis of Settlement Distribution on  Slope in Depok City
Abstrak: Pertumbuhan penduduk yang pesat serta bertambahnya tuntutan kebutuhan masyarakat akan lahan, seringkali mengakibatkan benturan kepentingan atas penggunaan lahan serta terjadinya ketidaksesuaian antara penggunaan lahan dengan rencana peruntukannya. Pembangunan perumahan yang dilakukan pada daerah bukan peruntukannya seperti daerah yang berlereng pada kenyataannya berbahaya dan dapat memperbesar ancaman bencana yang mungkin terjadi di kemudian hari seperti tanah longsor. Penelitian ini dilakukan selama bulan Februari-Juni 2021. Lokasi objek penelitian berada di Kota Depok. Penelitian diawali dengan studi pustaka. Langkah selanjutnya yaitu menentukan lokasi penelitian. Setelah lokasi penelitian ditentukan, dilanjut dengan mengumpulkan data sekunder. Pengolahan data yang dilakukan berupa analisis penggunaan lahan, uji akurasi, analisis sebaran permukiman, analisis kemiringan lereng, dan evaluasi sebaran permukiman terhadap kemiringan lereng. Luas sebaran permukiman di kelas kemiringan lereng 25%-45% dan diatas 45% berturut-turut sebesar 37,81 hektar dan 4,68 hektar. Pada kemiringan tersebut tidak baik untuk dibangunnya perumahan. Upaya mitigasi yang dapat dilakukan adalah dengan mengembalikan fungsi hutan di lereng-lereng bukit yang telah digunakan serta mengendalikan pembangunan (properti) sesuai dengan daya dukung lingkungan., Rapid population growth and increasing demands for land needs, often resulting in conflicts of interest over land use and the occurrence of discrepancies between land use and its allocation plan. Housing development carried out in the area is not the designation as the marbled area is in fact dangerous and can magnify the threat of disasters that may occur in the future such as landslides. This research was conducted during February-June 2021. The location of the research object is in Depok City. The research began with a library. The next step is to determine the location of research. Once the research location is determined, it is continued by collecting secondary data. Data processing conducted in the form of land use analysis, accuracy test, settlement distribution analysis, slope analysis, and evaluation of settlement distribution to slope. The distribution area of settlements in the slope class of 25%-45% and above 45% respectively is 37.81 hectares and 4.68 hectares. On that slope is not good for the construction of housing. Mitigation efforts that can be done is to restore the function of forests on the hillsides that have been used and control the development (property) in accordance with the carrying capacity of the environment.
Keyword: Mapping, settlement, slope

Judul: Analisis Perubahan Penggunaan Lahan dan Perkembangan Wilayah pada Lingkungan Kota Depok
Abstrak: Kota Depok mengalami perkembangan wilayah dan urbanisasi yang dipengaruhi perluasan perkotaan Jakarta. Hal ini terlihat dari adanya perubahan penggunaan lahan pertanian ke non pertanian yang berdampak pada kualitas lingkungan. Penelitian ini bertujuan mengidentifikasi perubahan penggunaan lahan tahun 2011-2018, menganalisis kualitas lingkungan dengan Local Environmental Sustainability Index (LESI) dan menganalisis faktor-faktor yang mempengaruhi kualitas lingkungan di Kota Depok. Analisis perubahan penggunaan lahan dilakukan dengan overlay (SIG) peta penggunaan lahan tahun 2011 dan 2018 serta menggunakan tabulasi matriks perubahan lahan. Penetapan Indeks Kualitas lingkungan (LESI) didasarkan metode yang dikembangkan Pravitasari et al. (2016). Faktor-faktor yang mempengaruhi kualitas lingkungan wilayah dianalisis dengan regresi berganda dimana salah satu variabelnya adalah indeks perkembangan wilayah (IPW) tiap kelurahan dari hasil skalogram. Ruang terbuka hijau, permukiman dan jasa/industri mengalami peningkatan luas dari tahun 2011 ke 2018. Luas lahan sawah dan pertanian lahan kering mengalami penurunan. Pengelolaan urbanisasi yang disertai bertambahnya lahan terbangun dan ruang terbuka hijau (RTH) di Depok telah semakin berpengaruh signifikan meningkatkan kualitas lingkungan di Kota Depok., Urbanization in Jakarta and its surroundings has influenced the development of Depok City. An indication of the ongoing urbanization process is the change in land use from agriculture to non-agriculture. These conditions can affect the land quality and environment. This study aims to identify land use changes in 2011-2018, to analyze environmental quality with the Local Environmental Sustainability Index (LESI) and to analyze factors that affect environmental quality in Depok City. Land use change analysis was carried out by overlaying (GIS) land use maps from 2011 to 2018 and using a land change matrix tabulation. The factors that affect the quality of the regional environment were analyzed by multiple regression in which one of the independent variables was the regional development index (IPW) of each urban village (kelurahan) from the scalogram results. Green open space, settlements, and services/industries have increased in area from 2011 to 2018. On the other hand, the area of rice fields and dry land agriculture has decreased. The increase in built-up land in Depok City shows that the urbanization process is being/has occurred. Depok City. The management of green open space (RTH) has significantly improved the environment quality.
Keyword: Urbanisasi, Lahan terbangun, Ruang Terbuka Hijau, LESI

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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