Judul: Keberadaan Mangrove dan Produksi Ikan di Desa Grinting, Kecamatan Bulakamba, Kabupaten Brebes
Abstrak: Ekosistem mangrove merupakan komunitas pantai tropis, yang didominasi oleh beberapa jenis vegetasi mangrove yang mampu tumbuh dan berkembang pada daerah pasang-surut pantai berlumpur. Hutan mangrove tersebut memiliki beberapa manfaat, namun manfaat yang paling penting adalah kaitannya terhadap perikanan pesisir, baik budidaya maupun nonbudidaya (tangkap). Tujuan penelitian ini untuk mengkaji kondisi mangrove, produksi ikan di kawasan mangrove dan kemungkinan adanya hubungan antara kondisi mangrove, kondisi lingkungan dan produksi ikan di Desa Grinting, Kecamatan Bulakamba, Kabupaten Brebes. Lokasi penelitian dilakukan di Desa Grinting yang merupakan sebuah desa pesisir yang terletak di Pantai Utara Kecamatan Bulakamba, Kabupaten Brebes. Penelitian dilakukan selama lima bulan, yaitu mulai dari bulan Juli – November 2009. Data yang dikumpulkan berupa vegetasi mangrove dominan, kualitas perairan dan produksi ikan (budidaya dan nonbudidaya). Analisis data diamati dengan uji statistik F untuk melihat perbedaan secara nyata antar Kanal. Kemudian dilakukan analisis komponen utama (principle componenet analysis/PCA) untuk melihat kedekatan hubungan dari parameter yang diuji dan mereduksi variabel yang paling berpengaruh.
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Judul: Relationship between Mangrove Ecosystem Conditions and  Fish Resource Diversity in the Mangrove Sari Tourism Area, Kaliwlingi, Brebes,  Central Java
Abstrak: Ekosistem mangrove merupakan salah satu ekosistem pesisir yang memiliki fungsi ekologi dan ekonomi penting. Salah satu fungsi ekologi adalah sebagai habitat bagi berbagai jenis ikan. Hubungan ekosistem mangrove dengan keanekaragaman ikan di Kawasan Wisata Mangrove Sari Brebes hingga saat ini masih belum banyak diteliti lebih lanjut. Penelitian ini bertujuan menganalisis keterkaitan antara struktur komunitas mangrove dan ikan di Kawasan Wisata Mangrove Sari Brebes. Penelitian dilaksanakan selama 4 bulan (November 2023 hingga Februari 2024) di Kawasan Wisata Mangrove Sari Brebes pada tiga stasiun pengamatan. Hasil penelitian menunjukkan bahwa kerapatan mangrove tertinggi di Stasiun 1. Ekosistem mangrove di Pandansari termasuk dalam kategori rapat dengan didominasi oleh mangrove jenis Rhizophora mucronata dan R. apiculata. Total hasil tangkapan ikan dari tiga stasiun sebanyak 4940 individu dengan keanekaragaman ikan dalam kategori sedang dan ikan tersebar secara merata serta tidak terdapat jenis ikan yang mendominasi. Kerapatan mangrove dengan keanekaragaman ikan di Kawasan Wisata Mangrove Sari memiliki hubungan yang positif., Mangrove ecosystem is a coastal ecosystem that has important ecological and economic functions. One of the ecological functions as a habitat for various types of fish. Relationship between the mangrove ecosystem and fish diversity in the Mangrove Sari Brebes Tourism Area has not yet been studied further. This research aims to analyze the relationship between the structure of mangrove communities and fish in the Mangrove Sari Brebes Tourism Area. Research was carried out for 4 months (November 2023 to February 2024) in the Mangrove Sari Brebes Tourism Area at three observation stations. The research results show that the highest mangrove density is at Station 1. Mangrove ecosystem in Pandansari is included in the dense category, dominated by mangrove types Rhizophora mucronata and R. apiculata. Total fish catch from three stations is 4940 individuals with fish diversity in the medium category and fish are evenly distributed and there are no dominating fish species. Mangrove density with fish diversity in Mangrove Sari Tourism Area have a positive relationship.
Keyword: Brebes, ikan, Tourism, Brebes, Fish, Mangroves

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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