Judul: Hubungan Pola Konsumsi, Status gizi dan Aktivitas Fisik dengan Kadar Asam Urat Lansia Wanita Peserta Posbindu Sinarsari
Abstrak: Arthritis is one of the diseases that are often found in women aged 55-70 years who often occured after menopause. The purpose of this study was to analyze the relationship between consumption patterns, nutritional status, physical activity with levels of uric acid in older women. Study design used in this study is a cross-sectional design. The sampling of this reasearh is 48 elderly people ≥50 years. There was significant difference (p<0.05) on age of menopause among the samples of normal and high uric acid level. There was no relationship between water consumption, coffee drinking habits, consumption of energy, carbohydrate intake, protein intake, fat intake, purines intake, nutritional status, physical activity and exercise habits with uric acid levels in the sample. There is a relationship between age at menopause with high levels of uric acid in the sample (p<0.05).
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Judul: Aktivitas Fisik, Pola Konsumsi Pangan, Status Gizi dan Tekanan Darah Hubungannya dengan Kadar Asam Urat pada Peserta Lansia Wanita Posbindu Bukit Duri Jakarta
Abstrak: Penelitian ini bertujuan untuk menganalisis hubungan antara aktivitas fisik, pola konsumsi pangan, status gizi dan tekanan darah dengan kadar asam urat pada peserta lansia wanita Posbindu Bukit Duri Jakarta. Desain studi yang digunakan yaitu cross-sectional study dengan jumlah contoh sebanyak 60 orang lansia wanita. Teknik pengambilan subjek yang digunakan adalah purposive sampling. Subjek adalah wanita usia lanjut ≥ 60 tahun, memiliki pendengaran yang baik, dapat diwawancarai, tidak mengonsumsi alkohol dan dalam keadaan sehat. Penelitian ini dilaksanakan pada Maret – Mei 2015. Hasil penelitian menunjukkan kadar asam urat contoh 68.3% tergolong normal dan 31.7% tergolong tinggi. Lebih dari setengah (56.7%) status gizi contoh tergolong normal. Sebagian besar contoh (61.7%) memiliki aktivitas fisik tergolong ringan dan 35% contoh memiliki tekanan darah tergolong hipertensi II. Berdasarkan uji korelasi spearman terdapat hubungan signifikan (p<0.05) antara aktivitas fisik, status gizi, tekanan darah, tingkat kecukupan vitamin C dan frekuensi konsumsi nasi dengan kadar asam urat. Tidak terdapat hubungan yang signifikan antara tingkat kecukupan energi, protein, lemak, karbohidrat dan asupan purin dengan kadar asam urat.
Keyword: aktivitas fisik, asam urat, lansia, pola konsumsi pangan, status gizi

Judul: Characterization and Expression of Tomato infectious chlorosis virus Coat Protein Gene in Escherichia coli
Abstrak: Tomato infectious chlorosis virus (TICV) is a member of Crinivirus genus. TICV infects tomato crop in Garut and Cipanas, West Java Indonesia. Tomato initially showed bright interveinal yellowing symptoms. The coat protein gene (CP-TICV) was amplified by RT-PCR from total RNA extracted from infected tomato leaves and the amplified fragment was cloned and completely sequenced. The fragment was subsequently subcloned into the pET-21b expression vector. The recombinant plasmid was transformed to Escherichia coli strain BL21(DE3)pLysS to express the coat protein. The coat protein fused to a 6xhistag, was purified by affinity chromatography using a NiNTA spin column. The identity of the purified protein was confirmed by SDS-PAGE. In this experiment, 792 bp of CP-TICV gene of TICV virus has been successfully cloned, sequenced, and expressed in E. coli. Based on nucleotide sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain strain and 99% identity to North America, France, and California strains and based on amino acid sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain, North America, France strains and 99.2% identity to California strain. CP-TICV showed over expressed in E. coli when it is induced with 1 mM IPTG and incubated at 37oC. Purified CP-TICV-Histag recombinants protein sized 29 kDa based on SDSPAGE analysis.
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