Judul: Kajian taman rumah tinggal berbasis budaya Madura
Abstrak: Indonesia merupakan bangsa yang memiliki keanekaragaman tinggi. Keanekaragaman tersebut tidak hanya dalam bentuk seni dan adat istiadat, tetapi juga dalam bentuk penataan ruang bentang alam. Dalam literatur dapat ditemukan berbagai kajian mengenai pola penataan ruang dari berbagai suku bangsa. Pola– pola keruangan tersebut merupakan salah satu bentuk kearifan lokal masyarakat dalam beradaptasi dengan alamnya sehingga membentuk suatu lanskap budaya. Pada umumnya pola keruangan dalam penataan lanskap berkaitan erat dengan karakter masyarakat atau suku bangsa yang tinggal di dalamnya. Penataan lanskap ini dapat tercermin dalam penataan ruang tinggal sebagai lingkungan terdekat bagi individu masyarakat. Hal inilah yang kemudian menjadi bahan kajian dalam penelitian desain taman rumah tinggal berbasis budaya ini. Salah satu suku bangsa Indonesia yang memiliki karakter khas adalah Suku Madura. Suku Madura mendiami pulau Madura dan sebagian Jawa Timur bagian utara. Mata pencaharian masyarakat umumnya bercocok tanam palawija. Lanskap yang tandus membentuk karakter masyarakat Madura sehingga dikenal sebagai manusia yang ulet dan keras kepala. Karakter ini tampak dalam pembawaan sehari–hari melalui interaksi dengan orang lain, sikap hidup, hingga pengaturan teritorialnya. Penelitian ini bertujuan mempelajari penataan elemen lanskap pada rumah tinggal masyarakat Madura serta filosofi yang mendasarinya sehingga dapat disusun suatu konsep taman rumah tinggal masyarakat Madura yang sesuai dengan karakter budaya Madura. Metode penelitian yang digunakan dalam penelitian ini adalah metode deskriptif dengan teknik pengumpulan data berupa studi pendahuluan, studi pustaka, dan wawancara. Wawancara dilakukan kepada narasumber dengan latar belakang budaya Madura cukup kuat dan diutamakan berdarah asli Madura. Selain itu, peneliti juga melakukan observasi lapang ke pulau Madura.
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Judul: Kajian Desain Taman Rumah Tinggal Tradisional Budaya Sunda
Abstrak: Sundanese culture is one of the oldest culture in Indonesia. The study of traditional Sundanese garden needs to be done in order to get a real image about traditional Sundanese culture. The objectives of this research are to identify the elements, layout, and interpretation of sundanese garden. This research was obtained through descriptive analysis by field observation and tracing the information of culture and history. The study area are Urug Village, Bogor District; Sindang Barang Village, Bogor District; and Naga Village, Tasikmalaya District. The result of this study showed the spatial order of traditional Sundanese house divided into three parts in according the Tritangtu concept; those are the front or upper part, the central part, and back or under part. Yard of Sundanese house divided into three parts, those are the frontyard (buruan), sideyard (pipir), and the backyard (kebon). The dominant open space is an expanse of land or a grass. The forming elements of traditional sundanese garden are leuit, saung lisung, traditional bathroom (tampian), pond (balong), garden light (obor), cage of hen, and plants.
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Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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