Judul: Struktur dan Keragaman Genetik Populasi Hama Boktor (Xystrocera festiva) pada Hutan Rakyat Sengon (Falcataria moluccana) di Jawa.
Abstrak: Boktor (Xystrocera festiva) is the most destructive sengon pests, because the larvae feeding activity is done by broaching the woody tissue inside sengon trees. The objective of this research was to study population structure and genetic diversity of boktor pests in Java using RAPD markers. Boktor larvae were sampled from 10 susceptible trees in eight districts in Jawa, and then census was done to calculate the total individuals found in each tree, before taking measurements of body length and diameter of its head. Having been census a number of 10 larvae per tree of various sizes were picked up as sample for DNA analysis. DNA extraction was done using CTAB (Cetyl Trimethil Ammonium Bromide) method, and the PCR (Polymerase Chain Reaction) was done using OPP 9, OPP 15, and OPP 19 primers to get RAPD (Random Amplified Polymophic DNA) fragment. Scored fragment data was analyzed using software POPGENE 1.31 version 32 and NTSYS version 2.0. The results showed the heterogeneity of the population structure according to the diameter and body length of each population. Result of RAPD analysis showed that the highest heterozigosity (He) value was from Tasikmalaya (0.2525) and the lowest heterozigosity (He) was 0.1039 from Magelang. Dendrogram based on genetic distance showed that Kediri population was closely related with Temanggung while Ciamis population is farthest.
Keyword: CTAB, RAPD, sengon, population structure, Xystrocera festiva

Judul: Analisis Keragaman Genetik Dan Filogenetik Populasi Hama Boktor Xystrocera Festiva (Coleoptera: Cerambycidae) Di Jawa
Abstrak: Banyak studi yang telah dilakukan untuk mencari sistem pengendalian hama boktor (Xystrocera festiva), yaitu hama utama pada tegakan sengon. Namun hingga saat ini sistem pengendalian yang ditemukan belum berhasil menangani serangan hama boktor. Hal ini disebabkan oleh minimnya informasi mengenai hama tersebut, salah satunya informasi mengenai variasi genetik pada hama boktor. Penelitian ini bertujuan untuk menganalisis pola sebaran serangan hama boktor pada delapan populasi sengon di Jawa, dan menganalisis variasi genetik larva yang ada di Jawa. Sampling dilakukan menggunakan metode purposive pada 8 populasi, yaitu Sukabumi, Cianjur, Tasikmalaya, Ciamis, Temanggung, Magelang, Kediri dan Malang. Variasi genetik boktor disekuen pada gen Cytochrome Oxidase subunit I (COI). Pola sebaran hama boktor di Jawa bervariasi di masing-masing populasi, yaitu beragamnya ukuran larva berdasarkan diameter kepala dan panjang tubuh yang mengindikasikan larva melakukan serangan berulang pada suatu populasi. Sekuen gen COI larva boktor yang berasal dari Jawa diperoleh sepanjang 605 pb yang terdiri dari 9 haplotipe yaitu 4 haplotipe diperoleh dari Jawa Barat, 3 haplotipe dari Jawa Tengah, dan 2 haplotipe dari Jawa Timur. Keragaman genetik X. festiva di Jawa termasuk kategori tinggi, yaitu Hd=0.772. Berdasarkan hasil analisis jarak genetik menunjukkan ilustrasi pohon filogenetik menunjukkan jarak genetik suatu populasi berbeda dipengaruhi oleh jarak geografis masing-masing populasi. Pohon filogenetik juga menunjukkan semua sekuen X. festiva berkelompok, dan berbeda dengan sekuen kelompok X. globosa yang diperoleh dari basis data Genbank. Hasil sekuen tersebut dapat digunakan sebagai barcode untuk membedakan spesies dari genus Xystrocera.
Keyword: Boktor, COI, populasi, sengon, variasi genetik

Judul: Hydroxyapatite-Chitosan Composite Coating on Stainless Steel 316 to Improve Corrosion Resistance
Abstrak: Metals that were used in bone implantation should have good corrosion resistance that were not easily corroded when implanted into the body. Metal corrosion resistance of stainless steel 316 can be enhanced through the metal coating by hydroxyapatite-chitosan composite with composition ratio of hydroxyapatite (gram) and chitosan 3% (mL) 0.2:0, 0.2:0.1, 0.2:0.5, 0.2:1.0, and 0.2:1.5. Besides improving the corrosion resistance of metal, the presence of chitosan also maintain the position of hydroxyapatite on metal surfaces. Corrosion resistance increases with increasing amount of chitosan added and the best composition of hydroxyapatite-chitosan composite was 0.2:1.0.
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