Judul: Analisis Keragaman Genetik Ikan Baung (Hemibagrus nemurus) dengan Menggunakan Teknik PCR-RFLP pada DNA Mitokondria
Abstrak: This research has an objective to analyze the genetic variation among four Hemibagrus nemurus population by using PCR-RFLP of D-loop region of mitochondrial DNA. H. nemurus were collected from Cirata, Serayu, Jambi and Bogor. Four restriction enzymes were used to digest D-loop region mt-DNA, i.e. MboI, HaeIII, AluI and RsaI. Based on this analysis, these four population have seven composite haplotypes. The highest haplotype diversity was found at the population from Jambi. Population from Serayu has just one composite haplotype indicating that this population has the same ancestor. Population from Cirata and from Bogor has the same composite haplotype indicating that there was probably migration between these two populations.
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Judul: Analisis keragaman genetik labi-labi, dogania Subpkma (Testudines: trionychidae), melalui teknik PCR-RFLP
Abstrak: Saat ini telah terjadi penurunan populasi labi-labi yang sebagian besar diakibatkan adanya perburuan liar dan kerusakan lingkungan. Melihat hal itu diperlukan suatu langkah untuk mengumpulkan berbagai informasi biologi labi-labi. Salah satu caranya adalah dengan melakukan anal isis molekular sebelum berbagai informasi lainnya diungkapkan. Penanda molekular genetik yang sering digunakan dalam analisis molekular adalah daerah pengendali (d-loop) genom mitokondria menggunakan teknik PCR-RFLP. Bagian yang paling stabil dari daerah pengendali mtDNA diamplifikasi secara in vitro dengan pasangan primer 577 dan 578. Produk PCR dipotong dengan lima macam enzim restriksi, yaitu Alu I, Hsp92 II, Hha I, Hpa II dan Rsa I yang menghasilkan tujuh haplotipe. Keragaman nukleotida antara haplotipe yang ditemukan di Jawa dan Sumatera (interlokasi) adalah 12.11 %. Angka itu jauh lebih besar dibanding keragaman nukleotida antar haplotipe di dalam Sumatera maupun Jawa (intralokasi) yang berturut-turut adalah 6.5% dan 3%.
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Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4