Judul: Analisis Keragaman Genetika Tanaman Kunyit dan Temulawak secara Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) Menggunakan Primer OPA-OPD 6-10
Abstrak: There are many studies on turmeric (Curcuma domestica Val.) and wild ginger (Curcuma xanthorrizha Roxb.) from Indonesia is only about the content of secondary metabolites, but research on the genetic diversity information of turmeric and wild ginger plants has never been done before. This study was aimed to analyze the fingerprint pattern of Indonesian native turmeric and wild ginger plants by using OPA-OPD 6-10 primer series to obtain a phylogenetic tree of these plants. The steps of this research were DNA isolation, amplification of DNA, then analysis and construction of phylogenetic trees using the UPGMA (unweighted pair group method arithmetic mean) with NTSYS software. The resulted DNA showed good bands and had a size between 11000-12000 bp with the range of purity between 1091-2625. DNA amplification with 20 random OPA-OPD 6-10 primers produced 259 bands (100% polymorphic). Analysis of genetic similarity indicated a genetic similarity coefficient 0.3033-0.6721 for turmeric plant and 0.1939-0.9082 for wild ginger plant. This value indicated that the turmeric and wild ginger plants from Indonesia has a high level of genetic diversity.
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Judul: Pola Keragaman Genetik Tanaman Kunyit (Curcuma longa Linn.) dan Temulawak (Curcuma xanthorrhiza Roxb.) Asal Pulau Jawa dengan PCR-RAPD
Abstrak: Turmeric (Curcuma longa Linn.) and Temulawak (Curcuma xanthorrhiza Roxb.) are Indonesian medicinal plants which nutritious for improving health. The pattern of genetic diversity and genetic relationship of these rhizome plants are not well known yet. The purpose of this research to analyze the pattern of genetic diversity and genetic relationship both plants using Polymerase Chain Reaction- Random Amplified Polymorhic DNA (PCR-RAPD). The sample used in this research include 6 turmeric plants from Bogor, Wonogiri, Karanganyar, Sukabumi and Ngawi; and 5 temulawak plants from Bogor, Wonogiri, Sragen, Karanganyar and Sukabumi. Stage of these research include DNA isolation; DNA amplification; and analysis pattern of genetic diversity and genetic relationship using NTSYS 2.02. The result of these research showed that isolation DNA was done successfully. The result of DNA isolation generally has a fairly good purity. The purity values of turmeric and temulawak in sequence were 1.093-1.417 and 1.044-1.544. The number of bands that arise from DNA amplification of turmeric and temulawak in sequence were 131 and 132 locus. The number of amplification bands mixed results and different sizes for each sample show the patterns of genetic diversity found in turmeric and temulawak plants. The closest genetic relationship showed by turmeric plants from Sukabumi and Ngawi; and temulawak plants from Bogor and Sukabumi. Genetic similarity coefficient ranged from turmeric plants and temulawak plants in sequence are 54%-92% and 55 %-83 %.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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