Judul: Persepsi, Motivasi, Sikap Dan Perilaku Masyarakat Lokal Terhadap Keberadaan Hutan (Kasus Di Kecamatan Gn. Kencana, Kabupaten Lebak, Propinsi Banten)
Abstrak: A good forest management is an important part to get sustainable forest in Gn. Kencana where “Baduy Luar” and non Baduy in local community live. The aim of this research is to analyze perception, motivation, attitude and behavior from Baduy Luar and non Baduy community in Gn. Kencana about managing their forest in their area. This research use purposive sampling to get 30 respondent and 3 village sampling (Cimanyangray, Sukanegara and Keramatjaya) in Gn.Kencana . Perception for land use is for farming area. Perception for wood can get from quantity from that forest, closed standing stock, and diameter of trees. And then, for perception of water, it can get from periods water flow, and clear of water condition. The result, Baduy Luar communities have better perception than non Baduy about this forest. Local communities have motivation to subtantiate for life and to increase economic value from forest resources. Actually, both of them are agree to conserve the land forest, source of wood and water supply. We can look that action from a part of Baduy Luar community get source of wood from twig or natural died tree. Altough non Baduy community will cut the trees when they want. Local wisdom still be a part of Gn. Kencana community when they use natural resources (in this case is forest), especially for Baduy Luar. Keywords : Gn. Kencana, Perception, motivation, attitude and behavior
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Judul: Kelembagaan Pengelolaan Sumber Daya Hutan Masyarakat Adat Baduy Desa Kanekes, Kecamatan Leuwidamar, Kabupaten Lebak, Propinsi Banten
Abstrak: Baduy is a traditional society in Indonesia who still keep the custom and tradition of their anchestor. Baduy society have uniqueness of traditional knowledge in their forest resources management. Its related with their tradition and the effect of anchestors credentials. The Baduy society lifelihood cannot be released from pikukuh (a guide, tradition, life regulation and credentials) that they obedient, which used for control their life. Pikukuh is a hereditary legal heir from their anchestors and never changed. Yet, the outside cultures developed make pikukuh changed, especially in Baduy Luar. In some cases, the Baduy society starts to leave their obedient to pikukuh, include the way to make decision about conservation in their society. The research purpose are to analyze the traditional knowledge and the experience of Baduy society in managing their forest resources, involving the conservation decision making. This research is a quality qualitative research which use the participant observation method by associate with the society and observe their social life. Eventhough a lot of the introduce of culture come inside, Baduy society have a traditional knowledge for generation. Baduy experiences in manage the foest give an effect balances in ecology
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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