Judul: Keragaman Gen CSN3 Ekson 4 pada Kambing Peranakan Etawah di BPTU KDI-HPT Pelaihari Kalimantan Selatan
Abstrak: Etawah grade goat as one of Indonesian local animal genetic resources has advantages, one of which is its high milk yield. CSN3 gene is a member of casein fragment related to protein and casein contents. The aim of this study was to identify polymorphisms of CSN3 in Etawah grade goat from BPTU KDI-HPT Pelaihari, South Kalimantan using the PCR-RFLP method. Polymorphisms of CSN3 gene were identified of 115 blood samples using PstI restriction enzyme that cuts the fragment of CTGCA|G. CSN3 amplification resulted 407 bp which is located at exon 4 was performed with annealing temperature 60 °C for 20 seconds. The results of PCR-RFLP analysis indicated that the analyzed CSN3 gene of Etawah grade goat which have monomorphic F allele was 100%.
Keyword: PCR-RFLP, Etawah grade goats, CSN3 gene

Judul: Eksplorasi Gen Growth Hormone Exon 3 Pada Kambing Peranakan Etawah (PE), Saanen Dan Pesa Melalui Teknik PCR-SSCP
Abstrak: This research was conducted to identify genetic polymorphism at the exon 3 growth hormone gene in three goat breeds. Polymorphisms at exon 3 growth hormone gene was identified by single strand conformational polymorphism polymerase chain reaction (SSCP-PCR) method. The DNA of 234 goat used were Etawah Grade (98 samples), Saanen (92 samples) and their crossbred (44 samples) in Cariu, Ciapus, Sukajaya, Cijeruk, Balitnak and Sukabumi. The PCR-SSCP method was performed at 250 V for 8 hours using 12% of acrylamide concentration. The result showed that the annealing temperature is 60 0C. The PCR product was 157 bp (base pair). The result SSCP method found four conformational patterns. The genotype frequency in exon 3 are AA (0,205), AB (0,856), AC (0,163) and BC (0,045). Beside that it was found three allele (allele A, B, and C). The highest frequencies were allele A (0,602) and B (0,443) at Saanen, Etawah Grade and their crossbred goat. The highest genotype frequency was AB at three goat breeds. The highest heterozygosity was found in Etawah Grade, Saanen and their crossbred goat (0,938). Exon 3 GH gene on three breeds goat have high polymorphism in six population.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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