Judul: Luasan Optimal Hutan Kota sebagai Penyerap CO2 dan Kesesuaiannya dengan Rencana Tata Ruang Wilayah di Kota Tangerang.
Abstrak: Tangerang City known as industrial area and residential area. The city has consumed a lot of energy, that lead to the increase of air pollution especially CO2 in atmosphere. One way to reduce level of air pollution is development of green open space, urban forest, and protected area within spatial planning of Tangerang City. The necessary of urban forest in the Tangerang City could be determined by CO2 absorption approach based on IPCC (Intergovermental Panel on Climate Change) method. The research aimed at estimating the optimal size of urban forest based on CO2 emission came from domestic, agriculture and industrial emission. Further, spatial suitability also were examined. Result showed that CO2 emissions amounted to 3188.9 Gg CO2/years in 2014. Meanwhile, the result of land cover classification revealed that the area of trees vegetation amounted to 1870.26 hectares and grass amounted to 1977.81 hectares. Both of trees vegetation and grass could only absorb 115.48 Gg CO2/years (3.62% of total emissions). The protected area established in land use plannning only 1421.06 Ha and therefore need a lot of additional urban forest, in fact there was no any urban forest established by the government.
Keyword: emission, spatial planning, urban forest

Judul: Penentuan Luasan Optimal Hutan Kota Berdasarkan Emisi Karbondioksida (CO2) di Kota Cilegon Provinsi Banten
Abstrak: Cilegon is one of the industrial cities that experience development are rapidly. Density of population activities such as transportation and industrial activity led to an increase in water and air pollution and temperature. This research was intented to know the optimal area of urban forest based on carbon dioxide emissions in the air that are generated by fuel oil, Liquid Petroleum Gas, coal, humans, cattle, and rice fields and also know the location of potentially managed as urban forest. methods of research conducted is the literature studies, interviews, preparation work, direct observation and ground check. Cilegon has eight locations of urban forest with a total area 26.11 ha. Carbon dioxide emissions are produced in Cilegon city amounted to 13240029 tons/year, necessitating the addition of city forest 227243.89 ha. The value of Carbon dioxide emissions in 2030 is 3924365870 ton, so land required is 67362298 ha. The potential for urban city is residential area, public cemetery, the beach, empty land industry with long term development plan, and road network.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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