Judul: Perubahan Struktur Penguasaan Lahan dan Pendapatan Keluarga Anggota Pengelolaan Hutan Bersama Masyarakat (PHBM)(Kasus LMDH Rimba Lestari, BKPH Ponorogo Selatan, KPH Lawu Ds, Kabupaten Ponorogo, Jawa Timur).
Abstrak: Program PHBM sangat berkaitan erat dengan pengelolaan dan pemanfaatan lahan hutan yang berpengaruh terhadap luas penguasaan lahan hutan dan berdampak pula terhadap pendapatan masyarakatnya. Tujuan dari penelitian ini untuk menjelaskan peran PHBM terhadap peningkatan dan distribusi penguasaan lahan dan pendapatan pada masyarakat Desa Slahung, Kecamatan Slahung, Kabupaten Ponorogo. Penelitian ini menggunakan metode survei dengan pemilihan responden dengan metode purposive sampling sedangkan pemilihan informan menggunakan metode snowball. Data primer dikumpulkan dari hasil wawancara terstruktur menggunakan kuisioner dan data sekunder diperoleh dari data pemerintah daerah setempat atau stakeholder terkait. Peningkatan penguasaan lahan dan pendapatan rumah tangga diukur dengan cara membandingkan luas penguasaan lahan dan jumlah pendapatan rumahtangga peserta PHBM sebelum dengan sesudah menjadi perserta PHBM. Distribusi penguasaan lahan dan pendapatan diukur dengan menggunakan indeks Gini. Hasil penelitian ini menunjukan bahwa program PHBM berpengaruh terhadap perubahan penguasaan lahan. Penambahan penguasaan lahan sebesar 0,30 ha pada rumahtangga yang termasuk kategori luas penguasaan lahan 0,2- 0,5 ha; sebesar 0,75 ha pada kategori 0,6-1,0 ha; dan sebesar 1,60 ha pada kategori >1,0 ha. Indeks Gini penguasaan lahan sebelum PHBM adalah 0,80 yang menunjukkan ketimpangan sangat tinggi; Sedangkan Indeks Gini selama mengikuti PHBM adalah 0,47 yang menunjukkan ketimpangan rendah. Dengan demikian distribusi penguasaan lahan diantara rumahtangga peserta PHBM menjadi lebih merata. Program PHBM juga meningkatkan pendapatan masyarakat. Pendapatan rata-rata rumahtangga sebelum PHBM sebesar Rp.335.583; Sedangkan selama mengikuti PHBM rata-rata pendapatan masyarakat sebesar Rp. 1.058.750. Indeks Gini pendapatan rumahtangga sebelum mengikuti PHBM sebesar 0,71; sedangkan indeks Gini pendapatan rumahtangga selama mengikuti PHBM sebesar 0,39. Dengan demikian distribusi pendapatan rumahtangga lebih merata.
Keyword: PHBM, Land Tenure Structure, Income Distribution, Gini Index

Judul: Evaluasi Pelaksanaan Program Pengelolaan Hutan Bersama Masyarakat (PHBM) LMDH Wana Bumi Tirta Makmur, Desa Banjaranyar, BKPH Margasari, KPH Balapulang, Perum Perhutani Unit I Jawa Tengah
Abstrak: Perum Perhutani as a state-owned companywho mandated to manage state forests are required to give great attention to the socio-economic problems of society, especially the rural people who mostly live arround the forest. An increase in market demand and the needs of the people who lives arround the forest for land that continue to increase for the sustainability of their lifes, making Perum Perhutanito conduct the forest resource management systems that involve the community who lives arround the forest with a system of Pengelolaan Hutan Bersama Masyarakat (PHBM).The purpose of this study is to analyze the public perception of PHBM program and obtain the information about the welfare level of the PHBM participants after join the PHBM program. The selection of respondents was done by a simple random sampling, which amounted to 30 respondents. An analysis of the respondents perception referred to the Likert scale and calculated using the basic statistic formula and assisted with the "Descriptive Statistics" Frequencies" application in SPSS 17, while for the analysis forthe relationship of respondents' income before and after participating in PBHM program was tested by using the "Compare Means "Paired -Samples T Test" applicationor the t-test in SPSS 17 software. PHBM program help to increase the income of rural forest communities whobecomes a pesanggemand have join in LMDH whichhold a partnership with the party of PerumPerhutani to participate in PHBM program. That income increase> 50% from Rp. 9,200,450, -/year to Rp. 14,495,950, -/year through the land provision by the local Perum Perhutani which can be tilled by the communities around the forest through the intercropping system. Villagers around the forest feel a huge benefit with the PHBM program, therefore the majority of the villagers have a good perception of the PHBM program with a percentage of 43.3%, followed the percentage of medium perception is36.7% and the percentageof low perception is 20 %. PHBM program also help the economy and improve the rural forest community welfare. The income according to the two criteria of welfare, the Sajogyo Poverty Line and the Tegal regency local minimum wage, most pesanggem not considered poor with percentage of 100% according to the Sajogyo Poverty Line and 56.67% by Tegal regency local minimum wage.
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Judul: Characterization and Expression of Tomato infectious chlorosis virus Coat Protein Gene in Escherichia coli
Abstrak: Tomato infectious chlorosis virus (TICV) is a member of Crinivirus genus. TICV infects tomato crop in Garut and Cipanas, West Java Indonesia. Tomato initially showed bright interveinal yellowing symptoms. The coat protein gene (CP-TICV) was amplified by RT-PCR from total RNA extracted from infected tomato leaves and the amplified fragment was cloned and completely sequenced. The fragment was subsequently subcloned into the pET-21b expression vector. The recombinant plasmid was transformed to Escherichia coli strain BL21(DE3)pLysS to express the coat protein. The coat protein fused to a 6xhistag, was purified by affinity chromatography using a NiNTA spin column. The identity of the purified protein was confirmed by SDS-PAGE. In this experiment, 792 bp of CP-TICV gene of TICV virus has been successfully cloned, sequenced, and expressed in E. coli. Based on nucleotide sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain strain and 99% identity to North America, France, and California strains and based on amino acid sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain, North America, France strains and 99.2% identity to California strain. CP-TICV showed over expressed in E. coli when it is induced with 1 mM IPTG and incubated at 37oC. Purified CP-TICV-Histag recombinants protein sized 29 kDa based on SDSPAGE analysis.
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