Judul: Karakterisasi Protein IgG Anti H5N1 Menggunakan Metode SDS-Page (Sodium Dodecyl Sulfate Polyacrilamide Gel Electrophoresis) Dari Kolostrum Sapi Yang Divaksin H5N1
Abstrak: This experiment was conducted to characterize immunoglobulin gamma (IgG) in colostrum of Friesian Holstein (FH) cow which vaccined by Avian Influenza virus vaccine subtype H5N1. Samples was divided into three sample, Kol I Sp4, Kol II Sp4, Kol III Sp4. These samples were compared to IgG control. IgG control was colostrum sample from unvaccinated FH cow. Characterization of IgG were done by SDS-PAGE method. The result showed that IgG control have three band proteins and the IgG colostrums from vaccinated cow have five to six band proteins. The molecular weight (MW) of these protein samples ranged between 19.49 to 228.09 kDa. There were differences between the molecular weight of sample protein Kol I SP4, Kol II SP4, and Kol III SP4 with IgG control. Based on the protein size bands, there were different between Kol I SP4, Kol II SP4, and Kol III SP4. There were some unidentified protein in the sample. Conclusion, MW of IgG from colostrum samples vaccinated are 147.37 kDa, 147.37 kDa, 140.57 kDa and the IgG control was 161.57 kDa.
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Judul: Pengaruh Ph Terhadap IgG Anti H5N1 Asal Kolostrum Sapi Yang Divaksin Dengan Vaksin Avian Influenza H5N1
Abstrak: The aims of this study is to determine the stability of IgG anti-H5 from bovine colostrum (BC) after exposure to acid and basic pH. Two samples of BC, whole colostrum and whey colostrum incubated at pH 4, 5, 9, and 10 for 30 and 60 minutes. Haemagglutination Inhibition (HI) test is used to determine IgG titer after incubation. IgG titers decreased after exposure to acid or basic pH. Acid exposure (pH 5) decreased antibodi titer of whole colostrum as much as 3,75% and 16,25% after incubation 30 and 60 minutes respectively. Antibody titer to pH 4 also decreased until 12,5% and 16,5% after for 30 and 60 minutes respectively. In whey colostrum, both pH 4 and pH 5 reduce 18,75% antibody titer. On other hand, IgG titers is decreased until 100% at basic pH (pH 9-10). In conclusion, IgG from BC is degraded in acidic and alkaline conditions.
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Judul: Rencana Pengelolaan Lanskap Kebun Raya Toledo, Ohio, Amerika Serikat
Abstrak: Kebun raya (atau bisa juga disebut kebun botani, taman botani) adalah suatu area kebun yang ditanami berbagai jenis tumbuhan yang ditujukan terutama untuk keperluan koleksi tanaman sebagai tempat penelitian dan pendidikan. Selain itu, kebun raya juga kerap kali digunakan sebagai sarana wisata bagi pengunjung. Kebun Raya merupakan tempat yang sangat sesuai untuk melakukan kegiatan magang dalam bidang pengelolaan lanskap. Jika ditinjau dari segi tujuan, kegiatan magang ini memiliki dua tujuan, yaitu: tujuan umum dan tujuan khusus. Secara umum, kegiatan magang dilakukan untuk mendapatkan pengalaman profesi, memperluas wawasan, dan mengasah keahlian dalam mempelajari fungsi lanskap kebun raya. Secara khusus, kegiatan magang dilakukan untuk mempelajari dan mengevaluasi proses pengelolaan lanskap kebun raya serta untuk mengevaluasi dan mengenalisis berbagai masalah yang muncul di lapangan.
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