Judul: Desain alun-alun kraksaan sebagai taman kota tradisional di Kota Kraksaan Jawa Timur
Abstrak: Kota Kraksaan merupakan kota baru yang baru saja ditunjuk sebagai Ibukota Kabupaten Probolinggo. Dalam lima tahun terakhir, perkembangan infrastruktur serta fasilitas di Kota Kraksaan mengalami peningkatan yang sangat pesat dibandingkan sebelumnya. Potensi pembangunan yang ada di Kota Kraksaan sangatlah menarik karena dapat mempengaruhi masyarakat Kraksaan khususnya. Lokasi yang membutuhkan perlakuan khusus salah satunya adalah Alun-Alun Kraksaan yang terletak di jantung Kota Kraksaan. Lokasi alun-alun ini memiliki potensi yang besar untuk menjadi landmark dari Kota Kraksaan. Selain itu, Alun-Alun Kraksaan sering disebut sebagai taman kota untuk tempat berinteraksinya masyarakat serta ruang terbuka hijau untuk menyeimbangkan pembangunan infrastruktur. Metode yang digunakan untuk penelitian ini menggunakan metode perancangan dari Gold (1980) dengan modifikasi yang meliputi inventarisasi, analisis sintesis, dan perancangan. Penelitian ini diharapkan dapat menjadi rekomendasi perancangan bagi pemerintah setempat untuk membangun alun-alun sebagai taman kota dengan berbasis nilai tradisional untuk Kota Kraksaan.
Keyword: Alun-Alun,, Design, Kraksaan City, Urban park

Judul: Pengelolaan dan Pengembangan Hutan Kota di Kota Kraksaan Kabupaten Probolinggo Jawa Timur
Abstrak: Kraksaan town as the capital of Probolinggo district had one urban forest. The purpose of this research it to identify the role in the management of Kraksaan’s urban forest and also determine the management concept of the urban forest including the type, form and function. This research was conducted between July –November 2013. The using method was in-depth interview with related maintaners and institutions, vegetation analysis, and literature studies. The management of Kraksaan’s urban forest was not going optimally because the Environment Department as the maintaner had not a clear management plan in accordance with PP No 63 year 2002 and there was no participation of the other stakeholders. The result of vegetation analysis indicated that varieties and evenness of the tree growth rate of species in the Kraksaan’s urban forest belonged to middle range and evenly. Local governments need to increase urban forest area by involving the various parties as well as its construction refers to the existence of environmental problems or potentially emergence in the future.
Keyword: management, Kraksaan’s urban forest, development

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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