Judul: Inspeksi User Interface pada Aplikasi Nelayan Pintar (Nelpin)
Abstrak: Kementrian Kelautan dan Perikanan meluncurkan aplikasi Nelayan Pintar (Nelpin) sebagai penunjang nelayan dalam memperoleh informasi mengenai kondisi laut dan pasar ikan. Penelitian ini bertujuan mengetahui nilai kesesuaian user interface Nelpin dengan kaidah mobile user interface yang dilakukan melalui inspeksi terhadap setiap komponennya. Kaidah yang digunakan yaitu Material Design, Designing Map Apps, dan Design Principles for Cartography. Inspeksi dilakukan melalui 3 tahap yaitu dekomposisi, inventarisasi komponen, dan pembahasan tiap komponen. Tahap dekomposisi menghasilkan 24 halaman. Inventarisasi dari semua halaman menghasilkan 15 komponen. Hasil inspeksi menunjukkan user interface Nelpin belum sepenuhnya memenuhi kaidah mobile user interface. Persentase kesesuaian antara komponen-komponen dengan kaidah tersebut rata-rata mencapai 47,64%. Persentase komponen tertinggi adalah Gambar Peta sebesar 80% dan terendah adalah Icon dan Margin sebesar 16,67%.
Keyword: kaidah, komponen, Nelpin, user interface

Judul: Pengujian Usability dan Black-Box Testing Aplikasi Nelayan Pintar Studi Kasus: Nelayan dan Penyuluh Tasikmalaya
Abstrak: Saat ini banyak kendala yang dihadapi oleh nelayan seperti kurangnya informasi kondisi alam serta infomasi mengenai harga ikan di pasaran. Untuk membantu nelayan mengatasi masalah tersebut, Badan Penelitian dan Pengembangan Kelautan dan Perikanan membuat sebuah aplikasi bernama Nelayan Pintar. Untuk menambah daya guna aplikasi ini, diperlukan serangkaian pengujian sistem. Ada 3 metode pengujian yang akan digunakan yaitu survei, think-aloud usability testing dan black-box testing. Dari hasil survei dan think-aloud usability testing, didapatkan poin-poin perbaikan aplikasi seperti perbaikan antarmuka, perbaikan peta dan keterangannya, serta perbaikan pada menu aplikasi. Dari hasil black-box testing, 4 dari 8 fungsi kompleks masih memiliki tingkat keberhasilan di bawah 50%. Namun terlepas dari semua rekomendasi tampilan untuk mempermudah pengguna, perbaikan terutama berada pada perbaikan source code dan aspek teknis lainnya.
Keyword: Nelayan, Pengujian Black-box, Usability, Think-Aloud, Survei

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.