Judul: Studi keberadaan roof garden terhadap kondisi iklim mikro di sekitar bangunan  Kasus di Kondominium Taman Anggrek, Grand Tropic Suite's Hotel, dan gedung Fakultas Kedokteran Universitas Trisakti.
Abstrak: Ketersediaan ruang terbuka hijau (RTH) merupakan salah satu usaha untuk mengatasi permasalahan panas kota dan pencemaran lingkungan di kota Jakarta. Saat ini penghijauan ke arah horizontal sulit untuk dilaksanakan karena terbatasnya lahan dan mahalnya harga lahan. Taman atap (roof garden) merupakan salah satu alternatif untuk meningkatkan kualitas lingkungan. Beberapa dekade terakhir, keberadaan roof garden sudah mulai tampak pada beberapa bangunan seperti apartemen, perkantoran, dan hotel. Tujuan dari penelitian ini yaitu untuk menganalisis keberadaan roof garden (struktur dan desain) dan pengaruhnya terhadap kondisi iklim mikro. Studi dilakukan pada bulan maret 2006 sampai dengan Agustus 2006, bertempat di Kondominium Taman Anggrek (KDTA), Grand Tropic Suite’s Hotel (GTSH), dan Fakultas Kedokteran Universitas Trisakti (FKUT). Penelitian ini bersifat deskriptif dan dilakukan dengan metode survei. Data yang diambil meliputi kondisi umum tapak, kondisi iklim mikro, struktur lapisan roof garden, media tanam serta elemen lunak.
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Judul: Studi Kasus : Kondominium Taman Anggrek dan Apartemen Roxy Mas
Abstrak: Penghijauan yang dilakukan untuk mengatasi masalah heat island di DKI Jakarta terasa belum maksimal akibat keterbatasan lahan yang dapat dimanfaatkan. Roof garden ditilik dari pengertian katanya yaitu taman di atas atap, temyata mampu menjawab masalah ketetbatasan lahan tersebut. Keberadaan roof garden sudah mulai tampak pada beberapa bangunan seperti apartemen, perkantoran dan hotel di DKI Jakarta. Mengingat fungsi ekologi dan estetika yang dihasilkannya, keberadaan roof garden tampaknya harus !ebi h disosialisasikan khususnya kepada masyarakat di perkotaan.
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Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.