Judul: Pemurnian dan Karakterisasi Enzim Glukoamilase dari Koji Merah (Monaseus anka IFO 3403)
Abstrak: Enzim glukoamilase merupakan enzim komersial yang memecah pati menjadi gula. Dalam penelitian ini, enzim glukoamilase yang diisolasi merupakan enzim yang diproduksi oleh kapang Monascus anka yang digunakan untuk memproduksi koji merah.
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Judul: Isolasi Dan Penapisan Kapang Penghasil Β-Glukosidase Dan Karakterisasi Enzim Yang Dihasilkan
Abstrak: Tumbuh-tumbuhan menghasilkan karbohidrat dalam bentuk pati dan lignoselulosa. Selulosa tidak bisa dimanfaatkan sebagai sumber energi oleh ternak unggas karena hewan tersebut tidak memiliki mikroorganisme rumen yang mampu mencerna selulosa. Beberapa cara pengolahan limbah dilakukan dengan menggunakan bantuan mikrob yang dapat menghasilkan enzim selulolitik. Selain kapang, enzim selulase juga dihasilkan oleh siput dan rayap. Penelitian ini bertujuan untuk mengisolasi kapang yang dapat menghasilkan enzim β-glukosidase, memproduksi dan mengkarakterisasi enzim. Sebanyak 22 isolat yang menunjukkan aktivitas β-glukosidase dengan menghasilkan daerah hitam pada medium yang mengandung eskulin, berhasil diisolasi. Kapang yang menghasilkan aktivitas β-glukosidase tertinggi (RM 3D) diidentifikasi sebagai Aspergillus foetidus (Naka.) Thom dan Raper. Produksi enzim β-glukosidase dari Aspergillus foetidus (Naka.) (RM 3D) pada medium yang mengandung 3% polard pada suhu ruang, dan waktu inkubasi 6 hari menghasilkan aktivitas sebesar 3.56 U/ml. Aktivitas optimum enzim β-glukosidase berada pada pH 5.0 dan suhu 60 ºC. Enzim β-glukosidase relatif stabil pada pH 4.2 - 5.0. Enzim β-glukosidase juga stabil pada penyimpanan suhu 28 dan 40 ºC, tetapi tidak stabil pada suhu 80 ºC. Aktivitas β-glukosidase meningkat dengan adanya penambahan kation-kation Mg2+, Ba2+, Ca2+, Mn2+, dan Co2+ dengan konsentrasi akhir 1mM. Aktivitas β-glukosidase meningkat setelah penambahan EDTA dan ion Fe2+ dengan konsentrasi akhir 5 mM. Aktivitas β-glukosidase menurun setelah penambahan kation Cu2+ dan Zn2+, baik dengan konsentrasi akhir 1 mM maupun 5 mM. Aktivitas spesifik β-glukosidase meningkat 2 kali lipat setelah pemekatan dengan menggunakan pelarut organik aseton pada tingkat kejenuhan 80%, tetapi kadar proteinnya menurun 64%. Hasil analisis SDS-PAGE memperlihatkan adanya 4 pita protein yang terbentuk, masing-masing dengan berat molekul 134.9, 104.7, 87.1, dan 41.7 kDa. Hasil analisis zimografi menunjukkan bahwa enzim β-glukosidase Aspergillus foetidus (Naka.) (RM 3D) mempunyai berat molekul 134.9 kDa.
Keyword: Karakterisasi, Energy sources, Celluldytic enzyme, Aspergillus foetidus, Polusacharide, Carbohydrate

Judul: Thermal Characteristics and Dynamics of Tropical Cyclone over Western North Pacific Ocean: A Case Study of Typhoon Songda (Chedeng) 2011
Abstrak: The frequent occasion of Tropical Cyclone (TC) over Western North Pacific (WNP) Ocean lies from 10˚N to 26˚N and 121˚E to 170˚E. Sources of TC development come from warm ocean temperature where latent heat is released during condensation processes and subsequently concentrated in the boundary layer. Relative humidity at 850-700 hPa is responsible to produce the most intense storm. Meanwhile, weak vertical wind shear between 850-200 hPa is one of the dynamical factors that lead to the development of TC. Pressure level of 850-200 hPa is chosen to minimize the thermodynamic effect in the lower troposphere. Typhoon Songda (Chedeng in the Philippines) or TSC lasted from May 18th to 30th, 2011 and strengthened into powerful Super Typhoon stage between May 24th and 27th. Long curving track and the exhibition of all sequences of TC life cycle, TSC is then considered as an ideal case of TC. The objective of the study with case of TSC is to investigate the role of thermal characteristics and dynamics on TC intensity. The research is carried out by using Advanced Research of Weather Research and Forecasting model (WRF ARW model) version 3.3. Two domains are employed in the model. The first domain situate from 02˚N to 40˚N and 118˚E to 149˚E and cover spatial resolution () of 33.1 km. The second domain situate from 09˚N to 27˚N and 119˚E to 132˚E with of 11 km. Timestep of the model () is 120 seconds. The model is supported by the following physical parameterization, i.e., Yonsei University Scheme (YSU) in the boundary layer, Kain-Fritsch scheme (KF) in the cumulus, and WRF Single-Moment 3-class scheme in the microphysics. Initial meteorological condition prior to the formation of the cyclone is generated using WRF ARW standard-release model. Sensitivity of the model output is obtained from a selected single-parameter change scenarios, namely, 10 % to 20 % decrease and increase of wind shear initial data at 850-200 hPa, and by 10 % to 20 % decrease of relative humidity initial data at 850-700 hPa. The result showed that the negative correlations between wind shear and intensity are found in wind shear scenarios. Wind shears of 10 ms-1 which lead to strengthening of the wind intensity in the early period of Typhoon development are found in wind shear scenarios. The effect of wind shear changes on wind intensity is statistically significant in the 20 % wind shear scenarios. During mature stage, weak wind intensity occurred when wind shear increase as given in the WRF standard-release and the 20 % decreasing scenario. Different situation is found in the 20 % increasing scenario where wind shear tend to weak and lead to strong wind intensity at this stage. Meanwhile, the reduction of moisture supply at 850-700 hPa resulting from 20 % decreasing relative humidity play a major role in the weakening of wind intensity and reduces mechanical energy of the cycle process as much as 300 J kg-1.
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