Judul: Konstruksi Gen Kitinase untuk Ketahanan terhadap Ganoderma pada Vektor Ekspresi pCambia 1303
Abstrak: Efforts to increase palm oil production face problems associated with pest and diseases caused by pathogenic fungus such as Ganoderma spp. A new approach to obtain palm oil transgenic is by inserting a gene encoding antifungi compound. One of genes is chitinase gene derived from Trichoderma. The research targets are to insert chitinase gene into expression vector pCambia 1303 between the 35S Cauliflower Mosaic Virus (CaMV) promoter and the root specific promoter. The Chitinase gene was cloned into pCAMBIA 1303 expression vector followed by transformation of recombinant plasmid into Escherichia coli XL-1 Blue and transferred into Agrobacterium tumefaciens. Results showed that the chitinase gene was successfully inserted into pCambia 1303. Restriction of the recombinant DNA using restriction enzymes Nco1 and Spe1 resulted in two bands of 1200 bp which is pCambia 1303 and of 1500 bp which is the chi gene. The recombinant DNA was successfully transformed into Agrobacterium tumefaciens using competent cell of AGLO strain. The success of transformation was analyzed by restricting recombinant DNA isolated from transformant colonies using NcoI and SpeI restriction enzymes.
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Judul: The Construction of pCAMBIA 1303-Stilbene Syntase Recombinant DNA Preventive for Rotten Root Disease of Oil Palm
Abstrak: An oil palm is one of the important estate commodities in Indonesia. Nevertheless the effort to increase its productivity has many hindrances. One of them is rotten root disease caused by Ganoderma spp. New approach to get resistence oil palm toward Ganoderma attack is to insert gene produces substance played role as an antifungi. One of genes that produces antifungi is stilbene syntase gene derived from grapes (Vitis vinifera). The purpose of this research is to cloning stilbene syntase gene on to pCAMBIA 1303 expression vector between 35S CaMV promoter. Stilbene syntase gene was cloned on to pCAMBIA 1303 expression vector then to be transformed on to Escherichia coli XL-1 Blue and the last to be inserted on to Agrobacterium tumefaciens. The result of this research shows stilbene syntase gene was successful to be cloned to pCAMBIA 1303. This result was showed by two band sized 12000 bp (pCAMBIA 1303 plasmid) and 1500 bp (stilbene syntase gene) in agarose gel after have been restricted with SpeI and NcoI enzyme.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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