Judul: Penetapan Kadar Air Kapasitas Lapang dengan Metode Alhricks, Drainase Bebas, dan Pressure Plate pada Berbagai Tekstur Tanah untuk Tanaman Bunga Matahari (Helianthus annuus L.)
Abstrak: Soil water availability is one of the important factors influencing plant growth. Field capacity is an upper limit of available water for plants. Field capacity can be determinated by different methods. The objectives of this research are to compare among methods of field capacity measurement and to observe a response of sunflower growth in the range of field capacity that define with different methods. This research was initiated with field capacity measurement with three diffrent methods, i.e. Alhricks, Free drainage, and Pressure Plate at different soil textures, i.e. clay, sandy clay loam, and sandy loam. Furthermore, sunflower planting with different watering treatment based on field capacity of each mentioned methods was carried out in greenhouse. The research was designed by completely randomized design factorial with three replications. The result showed that soil texture and field capacity method and interaction of both significantly affected a field capacity value. In clay textured soil, watering treatment based on field capacity of each method did not significantly affect on plant height, number of leaves, stem diameter, and dry root biomass but significantly affected on wet root biomass, wet and dry canopy biomass, and root length. The result also showed that different texture will affect growth response of sunflower.
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Judul: Perlakuan berbagai taraf kadar air terhadap pertumbuhan bunga matahari (Helianthus annuus L.) pada tanah paleudult tipik Parung dan hapludert tipik Ngawi
Abstrak: Ketersediaan air merupakan faktor utama dalam upaya mencapai produktivitas lahan kering yang maksimum. Penelitian ini bertujuan untuk mengetahui pengaruh tipe mineral liat dan perlakuan berbagai taraf kadar air terhadap pertumbuhan bunga matahari. Penelitian ini menggunakan dua jenis tanah, yaitu Paleudult Tipik Parung yang mempunyai tipe mineral liat 1:1 dan Hapludert Tipik Ngawi yang mempunyai tipe mineral liat 2:1. Kadar air diberikan 5 taraf pada masing-masing jenis tanah, yaitu 70%, 80%, 90%, 100%, dan 110% dari kapasitas lapang. Rancangan percobaan yang digunakan adalah rancangan faktorial 2 x 5 di dalam acak lengkap. Untuk membandingkan pengaruh masing-masing perlakuan tersebut dilakukan uji Beda Nyata Terkecil. Pada tahap awal dilakukan pengukuran kapasitas lapang dengan metoda Alhricks. Kemudian dilanjutkan dengan penanaman bunga matahari di rumah kaca, pengukuran tinggi tanaman dilakukan pada setiap minggu. Setelah tanaman mencapai pertumbuhan vegetatif maksimum, tanaman tersebut dilayukan dan diukur kadar airnya. ..dst
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Judul: Mitochondrial DNA Gene Organization and Phylogenetic Relationship of Naja siamensis
Abstrak: Naja siamensis is one of Asiatic cobra in the Family of Elapidae, known as a cryptic snake. It has many morphological variations that lead to misidentification. Misidentification can cause the wrong concept in developing antivenom types due to high snakebite cases occur in Thailand. Thus, an accurate species identification using a molecular approach is crucially required. Mitochondrial DNA has been used widely in snake molecular studies. These studies were aimed to determine the arrangement of the N. siamensis partial mitochondrial genes using reptile-oriented primers and to reveal the phylogenetic relationship among other species of Asian cobra. DNA extraction was carried out based on the standard DNA salting-out protocol and was conducted by bioinformatics analysis using BLASTn and MEGA 6 software. Gene annotation was performed against N. kaouthia complete genome. A total of 3121 bp of N. siamensis mtDNA genes were sequenced consisting of partial of six regions: 16S rRNA, ND1, tRNA-Ile, CRII region, tRNA-Leu, tRNA-Gln, and COI genes. The mtDNA gene organization of N. siamensis was predicted to has a similar pattern with the common Elapidae mtDNA gene organization. This study showed that there is a monophyletic form in Naja genus and N. kaouthia is the closest species with N. siamensis based on partial 16S rRNA and ND1 gene.
Keyword: 16S rRNA, Elapidae, ND1, phylogenetic relationship