Judul: Analisis Keragaman Genetik Ganoderma spp. Menggunakan Penanda Molekuler Random Amplified Polymorphic DNA (RAPD)
Abstrak: Information on genetic diversity of Ganoderma spp. as a cause of root rot disease in crops is necessary in the development of protection technique from Ganoderma disease. The objectives of this research is to study the genetic diversity of Ganoderma spp. that associated with cacao and its shade trees (sengon, mahoni, and lamtoro) by random amplified polymorphic DNA (RAPD) marker analysis. DNA amplification using 10 arbitrary oligonucleotide primers produced 220 polymorphic DNA fragments which are 100% polymorphic. The cluster analysis showed that 45 number of Ganoderma samples had a high variability with the coefficient value ranged from 0.71 to 0.91. Further analysis using Winboot software showed that three groups of Ganoderma spp. had a high degree of confidence (>50%), which were Ganoderma samples from sengon Tasikmalaya, sengon Palembang, and mahoni Jember; whereas the other groups of samples had a low degree of confidence (<50%).
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Judul: Identifikasi Ganoderma spp. Menggunakan Teknik DNA Barcoding
Abstrak: Oil palm is one of comodity plantation which is very important. One of the problems faced by the oil palm plantations are disease, namely Basal Stem Rot (BSR) which caused by Ganoderma spp. If plants were attacked by BSR, sooner or later, they would die and the production will decreased significantly. Consequently, in order to have an effective control of Ganoderma, an accurate identification is needed. This could be achieved by molecular techniques through DNA Barcoding method. The aim of this study was to is to find a specific primer for the identification of Ganoderma spp. that attack estate crops and their shade trees. Based on this research, it showed that the primer pair ITS1_F/ITS4_B has a high percentage of the ability to identify isolates of Ganoderma spp. compared with the other primer pairs, which are primer BenA F/ BenA_R, Gan_ITS1_F/Gan_ITS1_R, CO1_PenF1/CO1_PenR1, CO1_B_F/ CO1_B_R, and Gan_ITS2_F/Gan_ITS2_R. The primer pair ITS1_F/ITS4_B had a potential as a DNA barcode for identification of Ganoderma species.
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Judul: Transisi Fasa Materi Hadronik ke Materi Kuark pada Inti Bintang Neutron.
Abstrak: Bintang neutron terdiri dari beberapa lapisan yang disusun oleh materi nuklir yang berbeda dengan kerapatan yang semakin tinggi ketika mendekati pusat bintang. Inti luar bintang neutron disusun oleh materi hadronik (nukleon, lepton, dan hyperon), sedangkan inti dalam bintang neutron disusun oleh kuark-kuark bebas (up, down, strange) yang saling berinteraksi. Tujuan penelitian ini adalah mempelajari bagaimana transisi dari inti luar ke inti dalam bintang neutron dengan menggunakan pendekatan RMF (Relativistic Mean Field) untuk materi hadronik dan CIDDM (Confined Isospin Density Dependent Mass) untuk materi kuark. Tekanan transisi diperoleh ketika potensial kimia barion materi hadronik dan materi kuark bernilai sama. Nilai tekanan transisi dengan parameter SU6-TM1 sekitar 87 MeV/fm3 dan diperoleh massa maksimum bintang neutron yaitu 1.92M dengan jari-jari sekitar 8.62 km. Nilai tekanan transisi dengan parameter SU6-G2 sekitar 53 MeV/fm3 dan massa maksimum bintang neutron yaitu 1.88M dengan jari-jari sekitar 8.16 km. Jenis transisi fasa adalah orde satu ditandai dengan diskontinuitas dalam kerapatan energi (adanya mixed phase). Parameter-parameter pada model nuklir yang digunakan menyebabkan perbedaan kekakuan persamaan keadaan (EOS) dari materi hadronik dan materi kuark sehingga nilai tekanan transisi dari fasa hadronik ke fasa kuark juga berbeda.
Keyword: bintang neutron, persamaan keadaan, transisi fasa