Judul: Temilok (Bactronophorus thoracites) glycogen extract as co-precipitant of deoxyribonucleic acid
Abstrak: Temilok (Bactronophorus thoracites) is one of Teredinidae or family of wood-borer shellfish in fact it has a fairly high polysaccharide content, particularly interesting source of glycogen. The objectives of this experiment are to determine KOH concentration, percentation of cationic resin Amberlite IR-120 Na and its stirring time to extract the glycogen from temilok flesh based on Nicoletti and Baiocchi glycogen polysaccharide extraction patent method, and the ability of the extracted glycogen as co-precipitant in the precipitation of deoxyribonucleic acid (DNA) of human femur. Nitrogen content of extracted glycogen was measured by kjeldahl method. Nucleic acid content of extracted glycogen were spectrophotometrically measured at 260 nm, 280 nm and 320 nm. Extracted glycogen samples was characterized by determining the glucose content quantitatively using phenol-sulphate (phesul) method which measured spectrophotometrically at 490 nm . The treatment of 100g temilok flesh with 40% potassium hydroxide and heated to 100 oC decreased the nitrogen content of extracted glycogen to 250 ppm. Treated the solution with 12% resin cationic Amberlite IR-120 and stirring for 16 hours at room temperature yielded 10,19±2,23% glycogen characterized by the lowest nucleic acids content which is 0,07 mg/mL. In order to asses the ability of the glycogen extract as co-precipitant of low copy number DNA, 2% solution of glycogen extract were added until 20 μL per 800 μL solution of femur DNA and 40 μL sodium acetate. Results revealed that by adding the extracted glycogen solutions into the first step of DNA precipitation procedure, the yield of DNA were not significantly affected at the level of 0,05 though the yield of DNA decreased against blanko about 0,006 ng/μL of 0,0401 ng/μL.
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Judul: Peran Ekstrak Etanol Tempe Terhadap Kadar DNA dan RNA Testis Tikus Jantan Usia 70 hari
Abstrak: Phytoestrogen is a subtance in soybean that have similiar structure with natural estrogen. Soybean is the main raw material of making tempe. Phytoestrogen are natural estrogen in isoflavon group. This study was conducted to investigate the role of tempe extract in reproduction performance of adult male rats. Nine male rats weaning age (21 days) were divided into 3 groups, control, treatment groups that were given a tempe extract 0.25 g/rat/day and 0.5 g/rat/day until ages of 48 days. Testicular wet weight, testicular dry weight, concentration of DNA levels, concentration of RNA levels, and sperms quantity in each group were measured. Data were collected at the age of 70 days and were analyzed using ANOVA test and Duncan test with 95% confidence interval (a=0.05).The results show that tempe extract of 0.25 g/rat/day could decrease the concentration of DNA levels and 0.5 g/rat/day increase the RNA levels and the sperm quantity.
Keyword: Bogor Agricultural University, tempe extract, male rats, rna, dna

Judul: Perencanaan Tapak Eks-Pasar Inpres Sebagaitaman Kota Subang Di Kabupatensubang Jawa Barat
Abstrak: Ketersediaan tamanpublik di Kota Subang saat ini masih terbilang kurang, sedangkan permintaan masyarakat akan tempat rekreasi di Kota Subang sendiri sangat besar. Sementara itu masih banyak lahan di Kota Subang yang dibiarkan tidak tertata dan tidak terawatt, salah satunya ialah tapak eks-Pasar Inpres Kabupaten Subang.Lahan eks-Pasar Inpres yang berada di pusat kota merupakan titik strategis untuk menjadi taman kota. Selain itu, perencanaan taman kota di lahan tersebut juga merupakan salah satu wacana dalamKeputusan Bupati Kabupaten Subang No. 644/KEP.1016-Kumdang/2002.Penelitian ini bertujuan untuk menyusun perencanaan taman kota di lahan eks-Pasar Inpres Kabupaten Subang sehingga memiliki nilai fungsional dan estetik yang baik. Fasilitas yang direncanakan pada taman kota inidisesuaikan dengan kondisi fisik dan sosial pada tapak. Metode yang digunakan dalam tahapan perencanaan tapak Eks-Pasar Inpres Kabupaten Subang ini adalah dengan pendekatan sumber daya dan aktivitas yang dikemukakan oleh Gold (1980). Pendekatan sumber daya meliputi aspek fisik dan biofisik, adapun pendekatan aktivitasmeliputi aktivitas yang ada pada masa lampau dan saat ini sebagai dasar pertimbangan. Faktor sosial lebih diutamakan daripada faktor lainnya dalam pendekatan ini. Perencanaan Taman Kota Subang dilakukan terhadap aspek tata ruang, sirkulasi,tata hijau dan fasilitas. Hasil perencanaan dipresentasikan dalam bentuk gambar rencana tapak (site plan).
Keyword: perencanaan tapak, rekreasi, Subang, taman kota