Judul: Penentuan Nilai Karakteristik Akuifer Sumur Air Tanah Melalui Uji Pemompaan (Pumping Test) Dengan Metode Cooper-Jacob di Leuwikopo, Darmaga
Abstrak: Air merupakan salah satu sumberdaya alam utama yang sangat dibutuhkan oleh seluruh makhluk hidup yang ada di alam semesta ini Tanpa adanya air maka akan terjadi banyak penderitaan dan kesengsaraan. Air permukaan dan air tanah yang umumnya sering dimanfaatkan, walaupun pemanfaatan air tanah (groundwater) belum optimal dibandingkan air permukaan (surface water). Hal ini dikarenakan air permukaan lebih mudah diperoleh dan dimanfaatkan. Akan tetapi, bila dilihat dari distribusi air di bumi tersebut maka air tanah memiliki potensi pemanfaatan yang sangat besar
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Judul: Penentuan Karakteristik Akifer dan Potensi Airbumi di Jakarta
Abstrak: Groundwater is one of the water resources used for domestic and industrial water uses, particularly in Jakarta region. Excessive extraction of groundwater has caused groundwater depletion and land subsidance. This study aimed to determine the characteristics of aquifer and groundwater potential of the Jakarta region based on available geologic logs and well data, including pumping test data. Analysis of geological log indicated that Jakarta region had several layers of confined aquifer namely aquifer 20 – 30 m below ground and another aquifer 50 – 170 m below the ground surface. Analysis result of the pumping test data from 14 production wells in Jakarta region indicated transmisivity value ranged from 5 to 522.8 m2/days, while the values of hydraulic conductivity of the aquifer system in Jakarta region ranged from 0.62 to 58.09 m/day. Hydraulic conductivity values indicated that the rock types of aquifer in Jakarta region were dominated by fine sands and clay. From the aquifer characteristic, potential groundwater in Jakarta region ranged from 5 to 522.8 m3/day/ meter aquifer. Groundwater potential of each well in Jakarta region was still not fully utilized due to improper pumping construction in positioning the well –screen-, where was not installed properly on aquifer layers but on clay layers, so the well efficiency become low with the consequences of high operation costs.
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Judul: Cloning and Structure Prediction of C-Type Lectin 19 (CTL-19) from Silkworm (Bombyx mori L.)
Abstrak: Bombyx mori L. is a highly significant insect due to its silk production affects several industries. Unfortunately, B. mori farming has recently experienced mortality issues caused by pathogenic infectious diseases. Despite the extensive study of innate immunity of B. mori, information regarding the molecular functions and mode of actions of C-type lectin 19 (CTL-19) in the innate immunity of B. mori remains limited. Therefore, this study aimed to predict the structure of the B. mori CTL-19 also clone and produce the recombinant protein to better understand its role in the silkworm's innate immunity. The open reading frame (ORF) of the CTL-19 gene (945bp) was successfully amplified and cloned to pGEM®-T Easy Vector. The mature protein consists of 294 amino acids with 2 CRDs, 9 conserved cysteine, and two specific carbohydrate binding motifs. Protein threading demonstrated to be superior in order to predict the ligand binding site in comparison to homology modelling. The predicted molecular weight is 33.26 kDa, with pI value of 5.70. Furthermore, molecular docking analysis reveal that BmCTL-19 have ability to bind to various carbohydrate residues. The BmCTL-19 gene will be sub clone into an appropriate expression vector and produce purified recombinant protein to evaluate its properties.
Keyword: Innate immunity, Carbohydrate Binding Motif, Homology modelling, Protein Threading, Molecular docking