Judul: Diversity of Longhorn Beetles (Coleoptera: Cerambycidae) and Moths (Lepidoptera) at IPB Campus Dramaga
Abstrak: Serangga adalah suatu organisme yang berperan petunting bagi lingkungan karena merupakan suatu indikator keseimbangan ekosistem, seperti kumbang antena panjang dan ngengat. Penelitian ini bertujuan menganalisis keberadaan kumbang antena panjang dan ngengat pada beberapa tegakan di Kampus IPB Dramaga, yaitu di Segitiga Silvikultur (SS), Arboretum FAHUTAN (AF), dan Taman Hutan Kampus (THK). Metode penelitian menggunakan Artocarpus trap sebagai perangkap kumbang antena panjang dan ngengat. Hasil penelitian menunjukkan bahwa kelimpahan kumbang antena panjang dan ngengat tertinggi di plot penelitian THK karena tersedianya sumber makanan yang cukup. Kelimpahan kumbang antena panjang sebanyak 27 individu dengan 5 spesies, sedangkan kelimpahan ngengat sebanyak 39 individu dengan 5 morfospesies. Kelimpahan kumbang antena panjang didominasi oleh spesies Acalolepta rusticatrix dan ngengat didominasi oleh famili Crambidae. Keberadaan kumbang antena panjang dan ngengat dipengaruhi oleh faktor klimatis, keanekaragaman pohon, dan aktivitas manusia. Beberapa spesies kumbang antena panjang juga diduga berpotensi sebagai bioindikator suatu perubahan ekosistem hutan karena memiliki habitat yang khas.
Keyword: bioindikator, ekosistem, klimatis, perangkap Artocarpus

Judul: Diversity and Abundance of Two Canopy Beetle Families in Four Landuse Types at Harapan Rainforest, Jambi.
Abstrak: Pembukaan lahan hutan menjadi kawasan perkebunan menyebabkan penurunan berbagai spesies flora dan fauna di wilayah tropis, tidak terkecuali di Indonesia. Salah satu yang terdampak besar adalah penurunan kelimpahan dan kekayaan spesies serangga, diantaranya kumbang Elateridae dan Staphylinidae. Penelitian ini bertujuan memelajari pengaruh perbedaan waktu dan perubahan tata guna lahan terhadap keanekaragaman dan komposisi kumbang Elateridae dan Staphylinidae di beberapa wilayah Hutan Harapan, Jambi, Sumatra. Pengambilan sampel dilakukan dengan menggunakan metode pengasapan kanopi pohon pada empat tipe penggunaan lahan: hutan, hutan karet, perkebunan karet, dan perkebunan kelapa sawit. Pada masing-masing tipe penggunaan lahan, terdapat empat plot inti dan pada setiap plot terdapat tiga subplot sebagai ulangan. Serangga dikumpulkan dalam delapan perangkap (ukuran 1 m × 1 m) yang dipasang di bawah tajuk tanaman sebelum proses pengasapan. Sampel yang telah dikumpulkan, disortir, dan diidentifikasi. Kumbang Elateridae ditemukan sebanyak 527 individu yang terdiri dari 3 subfamili, 10 genus, dan 18 morfospesies, sedangkan kumbang Staphylinidae ditemukan sebanyak 694 individu yang terdiri dari 11 subfamili, 16 genus, dan 47 morfospesies. Perbedaan tipe penggunaan lahan mempengaruhi kekayaan spesies kumbang Elateridae dan Staphylinidae, tetapi perbedaan tipe penggunaan lahan tidak mempengaruhi kelimpahan spesies kumbang Elateridae dan Staphylinidae., Transformation of forest land into plantation areas has resulted in the decline of various species of flora and fauna in the tropics, including Indonesia. One of the major impacts is the decrease in the abundance and richness of insect species, including Elateridae and Staphylinidae beetles. This reserach was aimed to study the effect of time and landuse change toward the diversity and abundance of Elateridae and Staphylinidae beetles in Hutan Harapan landscape in Jambi, Sumatra. Sampling was carried out using the tree canopy fogging method on four types of land use: forest, jungle rubber, rubber plantation, and oil palm plantation. In each land use type, there are four core plots and in each core plot, there are three subplots. Insects were collected in 8 traps (1 m × 1 m) installed under the plant canopy before the fumigation process. Samples that have been collected, sorted, and identified. The Elateridae beetles were found in as many as 527 individuals consisting of 3 subfamilies, 10 genera, and 18 morphospecies. While Staphylinidae beetles were found as many as 694 individuals consisting of 11 subfamilies, 16 genera, and 47 morphospecies. Different types of land use affect the species richness of Elateridae and Staphylinidae beetles, but differences in land use types do not affect the abundance of Elateridae and Staphylinidae beetles species.
Keyword: arboreal, keanekaragaman hayati, transformasi habitat, arboreal, biodiversity, habitat transformation

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
Keyword: