Judul: IPB (Bogor Agricultural University)
Abstrak: Kucing lokal (filis domestica) merupakan salah satu jenis hewan kesayangan yang sering digemari dan dipelihara orang. Jenis kucing ras tertentu seperti ras Persian, Anggora, Chincila dan Abyssinian mempunyai nilai ekonomis tersendiri. Populasi kucing Iokal sangat banyak dan mudah didapat khususnya di Indonesia, daya adaptasinya cukup baik dan memiliki kemampuan reproduksi yang cukup tinggi (multipara). Hewan ini tergolong ke dalam Ordo Carnivora dan Famili Fellidae seperti harimau. Bentuk fisik, fisiologi dan biologi reproduksinya mempunyai banyak kesamaan sehingga dapat. dipakai sebagai hewan model dalam upaya penelitian sebagai salah satu upaya ke arah konservasi satwa langka.
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Judul: IPB (Bogor Agricultural University)
Abstrak: Cacing tanah E. foetida merupakan salah satu spesies yang mempunyai habitat asli pada limbah organik terutama feses ternak. Masatah utama yang dihadapi alam budidaya cacing tanah E. foetida adalah teknologi budidaya yang diterapkan oleh peternak belum sesuai dengan persyaratan kehidupannya, sehingga produktivitasnya rendah. Hal ini disebabkan karena cacing tanah termasuk hewan poikilotermik yang kehidupannya sangat dipengaruhi oleh &ktor-faktor lingkungan. Di antara faktor-faktor lingkungan yang berpengaruh adalah ketersediaan pakan, pH, suhu, kelembaban, aerasi media, cahaya, kepadatan populasi dan predator.
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Judul: Glucose biosensor using a glucose dehydrogenase flavin adenine dinucleotide electrochemically immobilized on zeolite nanoparticles
Abstrak: A glucose biosensor was developed to measure blood glucose levels. The performance of these new glucose biosensors was continuously improved, producing better performance and stability. The technique selection and immobilization matrix are key factors for biosensor development, so that the prospecting of materials that can be used as a matrix for supporting enzyme, continue to be explore. The purpose of this study was to determine the activity and stability of the enzyme glucose dehydrogenase (GDH) that were immobilized on zeolite nanoparticles (NPZ) and determine the kinetic parameters and analytical performance by electrochemical methods. The resulting enzyme electrode with NPZ and enzyme electrode with zeolite A showed a high activity against the enzyme electrode glucose than without NPZ. The reaction kinetics of third electrodes of enzyme followed the Dixon equation. The 𝐼����𝑚����𝑎����𝑥����𝑎����𝑝����𝑝���� values for enzyme electrode without NPZ, with NPZ, and with zeolite A were 27.25 μA, 77.52 μA, and 48.54 μA, respectively. The 𝐾����𝑀����𝑎����𝑝����𝑝���� values for GDH enzyme that immobilized on the NPZ were 1.16 mM, 12.30 mM for GDH enzyme that immobilized on zeolite A and 0.95 mM for GDH enzyme immobilization without NPZ. The enzyme electrode without NPZ, with NPZ, and with zeolite A also showed a high sensitivity with value for the sensitivity were 0.58 μA mM-1, 0.81 μA mM-1, and 2.51 μA mM-1, respectively. The third enzyme electrodes showed different linear area with range for the enzyme electrode without NPZ, with NPZ and with zeolite A were 1.00-15.00 mM, 2.00-20.00 mM, and 0.06-10.00 mM, respectively. The blood glucose level of normal person ranged from 4 to 6 mM, so that the enzyme electrode with NPZ and without NPZ are suitable for use in the determination of the concentration of blood glucose. While the enzyme electrode with zeolite A has a smaller range so it is more suitable to use in the determination of glucose concentration in the foods or beverages. The enzyme electrode with NPZ and the enzyme electrode with zeolite A showed a good accuracy with relative error values were 3.84% and3.48%, respectively. Repeatability measurements also showed a good precission results with relative standard deviation values (%RSD) for the enzyme electrode with NPZ and enzyme electrode with zeolite A were 3.83% and 3.16%, respectively. By combining two immobilization techniques, physical adsorption on zeolite and entrapment on carbon paste, high enzymatic activity, good accuracy, and repeatability was attained. The enzyme electrode with NPZ and the enzyme electrode with zeolite A demonstrated a good electrochemical response toward glucose, allowing it to detect blood glucose levels and glucose levels in foods and beverages.
Keyword: glucose biosensor, immobilization, zeolite