Judul: Aktivitas Antibakteri dan Antioksidan Peptida Bioaktif Yoghurt yang Difermentasi dengan Lactococcus lactis Rekombinan
Abstrak: Yoghurt merupakan pangan fungsional yang dipercaya punya efek terhadap kesehatan. Efek tersebut salah satunya dikaitkan dengan keberadaan peptida bioaktif hasil fermentasi susu oleh bakteri asam laktat. Lactococcus lactis rekombinan merupakan bakteri asam laktat yang dapat menghasilkan plantarisin. Plantarisin diketahui memiliki efek antimikroba dan telah diuji sebagai bahan pengawet pada beberapa jenis pangan. Penelitian ini bertujuan menguji aktivitas antioksidan dan antibakteri yoghurt yang telah ditambahkan bakteri rekombinan L. lactis sebagai kultur starter. Selain itu juga untuk mengukur perubahan aktivitasnya setelah penyimpanan. Water soluble peptide extract (WSPE) yoghurt didapatkan dari hasil sentrifugasi dingin dan pemekatan dengan freeze dry. Kadar protein WSPE diukur terlebih dahulu dengan BCA kit sebelum dilakukan uji lanjutan. Yoghurt D hasil penyimpanan dengan total protein 5.226 mg/mL memiliki aktivitas antibakteri paling baik dengan zona bening 6.5 mm dan aktivitas antioksidan 20.11%. Hasil konfirmasi protein dengan trisin SDS-PAGE menunjukkan telah terjadi hidrolisis protein α-S2kasein (25.76 kD) pada sampel WSPE yoghurt D segar maupun penyimpanan.
Keyword: Antibakteri, Antioksidan, Peptida Bioaktif, WSPE

Judul: Aktivitas protease dan kandungan asam laktat pada yoghurt yang dimodifikasi Bifidobacterium bifidum dan diinokulasi pseudomonas fluorescens
Abstrak: Main health benefical related to yoghurt is its ability to improve lactose digestion and to control the pathogenic bacteria in digestion tract. This benefical health is threatened by natural existence of contaminant in milk especially P. fluorescens, that reduces shelf life of milk. The aim of this research is to determine the antagonistic nature of B. bifidum in inhibiting growth P. fluorescens and the effect of B. bifidum on protease activity and lactic acid content of yoghurt inoculated by P. fluorescens. Antagonism test of B. bifidum to P. fluorescens is conducted using turbidimetric method. Total titratable acid was measured to determine the percentage of lactic acid content by using acid base titration principle. Protease activity was measured with modification of Lowry method. The result of antagonism test showed that B. bifidum can inhibit P. fluorescens with minimum inhibitory concentration (MIC) of 2%. Lactic acid content of yoghurt with formula 1:1:0, 1:1:0.5, 1:1:1, and 1:1:1.5 on 0 day was 0.3747%, 0.3654%, 0.3732%, and 0.347% respectively and then increased during 25 day storage to 0.5709%, 0.5202%, 0.6638%, and 0.5945% respectively. Addition of B. bifidum decreased protease activity indicated by less protease activity of yoghurt modified by B. bifidum till 15 day storage compared to yoghurt without B. bifidum.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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