Judul: Fisiologi Pertumbuhan dan Pembungaan Tanaman Manggis (Garcinia mongostana L.) Asal Biji dan Sambungan
Abstrak: Mangosteen trees from seedling grow faster, but have longer juvenile period than the grafted trees. In contrast, grafted trees grow slower, but begin to flower earlier. The research was conducted to study the physiology of growth and flowering of seedling mangosteen.
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Judul: Perkembangan Morfologi dan Fisiologi Buah Manggis  (Garcinia mangostana L.) selama Pertumbuhan dan  Pematangan
Abstrak: Manggis merupakan tanaman asli Indonesia dan tersebar hampir di seluruh pulau di Indonesia. Buah manggis selain dikonsumsi sebagai buah segar dan minuman (jus), juga memiliki khasiat sebagai obat. Perikarp buah manggis memiliki keragaman kimia organik yang kompleks, diantaranya yang terkenal adalah asam tannin dan santonin yang dapat berperan sebagai anti inflammatory, anti bakteri, dan anti kanker. Indonesia merupakan eksportir terpenting buah manggis di dunia, dan untuk mendukung daya saing industri manggis di Indonesia diperlukan kajian ilmiah dalam hal perkembangan morfologi dan fisiologi buah manggis selama pertumbuhan dan pematangan untuk mendapatkan buah manggis yang berkualitas tinggi dan memenuhi standar. Mekanisme pembentukan biji manggis berbeda dengan kebanyakan tanaman pada umumnya. Biji manggis terbentuk tanpa melalui proses penyerbukan (polinasi) dan tanpa penggabungan gamet (fertilisasi). Berdasarkan reproduksi tersebut, maka manggis digolongkan sebagai buah apomiksis. Sampai saat ini penelitian mengenai perkembangan morfologi dan fisiologi bunga dan buah manggis masih sangat langka sehingga menarik untuk dikaji. Pemahaman mengenai perkembangan morfologi dan fisiologi buah manggis selama proses pertumbuhan dan pematangan sangat diperlukan sebagai landasan ilmiah untuk menentukan waktu panen yang tepat dengan kualitas hasil yang tinggi, mengingat sampai saat ini pemanenan buah manggis di tingkat petani umumnya hanya berdasarkan perubahan warna kulit buah sehingga sulit ditentukan waktunya dengan tepat.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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