Judul: Keragaman Genetik Tanaman Penghasil Gaharu Aquilaria malaccensis berdasarkan Penanda Mikrosatelit
Abstrak: Aquilaria malaccensis is a high quality agarwood producing tree of which population in nature has become rare and almost extinct due to over-exploitation. This research aim to estimate the genetic variation of innoculated A.malaccensis seedling and tree populations using microsatellite marker and to determine the correlation between the plant genotypes and the agarwood components based on analysis using GCMS. DNA was extracted using Dneasy plant mini kit from QIAGEN, and then amplified using 4 microsatellite primer pairs, i.e. 6pa18, 10pa17, 14pa17, and 16pa17. However, only two primer pairs, i.e. 6pa18 and 16pa17 were able to cross-amplify and produced polymorphic fragments with different sizes. In microsatellite locus of 16pa17 the presence of one specific allele was detected which could differentiate the plant that potentially able to produce the large amount of agarwood. The estimated value of genetic variation of the populations studied was categorized as high (He = 0.31). This variation was spread as much as 63% within populations and 37% among populations.
Keyword: microsatellite, genetic variation, genetic structure, A.malaccensis

Judul: Karakterisasi interaksi antara tanaman aquilaria microcarpa baill dengan fusarium solani dalam pembentukan gaharu
Abstrak: Aquilaria microcarpa is native to Indonesia and has been identified and promoted as potential tree species to yield high quality agarwood (gaharu). Individual trees of this species, both in its natural habitat and plantation, do not always yield agarwood due to many influencing factors. It is estimated that only 10% trees would be able to yield the resin. A clear mechanism of agarwood formation is unknown and there is a lack of information on underlying causes and triggers of the formation due to natural complexity and uncertainty. However, natural defense mechanisms through a series of pathogenesis are presumed as naturally observed in other resin producing tree species. Research on the characterization of host-pathogen interaction in gaharu formation was carried out based on the above mentioned problems with the following specific objectives, namely: i). to identify morphological characters of A. microcarpa trees interacting with F. solani sp, ii). to determine wood anatomical characters of A. microcarpa interacting with F. solani, and iii) to verify the genotypes of A. microcarpa seedlings and trees interacting with F.solani based on microsatellites. Results on tree morphological analysis showed that two out of 17 morphological characters (descriptor), namely tree height (m) and branching angles (0), showed significant differences between inoculated and non-inoculated individuals. In addition, microscopic observations on wood anatomical characters of A. microcarpa revealed that inoculated plants clearly deposited golden-brown colored resin in pores and canticles with a distinct scent. Further verification using GC MS on the sediment deposits identified common compounds as reported in many previous research on agarwood, namely: baimuxinal, elemol, 3-phenyl-2-butanone and chromone-3-one. On the other hand, tests on the viulence degrees of F. solani showed that one out of 4 strains, i.e. F.solani FORDA 512, showed the highest level of virulence and was significantly different with other F.solani, especially if inoculated on seedlings A60, A68 and B30. Two microsatellite loci of A. crassna, i.e. 6Pa18 and 71Pa17, were successfully transferable to A. microcarpa and could be used to characterize the genotypes of Fusarium-treated seedlings (n=40), inoculated trees (n=20) and non-inoculated trees (n=20). There were differences in terms of genetic structures between tree population and seedling population. Further sequence analysis on the microsatellite fragments on the most virulent F.solani FORDA 512- inoculated seedlings indicated genotype dependant responses for both seedling and tree stages. In addition, Sequence based cladogram showed that inoculated seedlings were closely placed with inoculated trees indicating certain genetic roles in the agarwood formation. In conclusion, tree morphological (i.e. height growth and branch habit) as well as genetic and genomic characters were clearly observed and useful to provide early information on gaharu formation. It is also supported by the wood anatomy analysis showing the presence of gaharu resin.
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Judul: Perhitungan Kebutuhan Tendik PNS Berdasarkan Analisis Beban Kerja Pada Biro Umum dan Keuangan Universitas Negeri Padang
Abstrak: Diketahui dalam rentang tahun 2022 hingga 2025 akan ada 6 orang tendik PNS yang pensiun pada BUK UNP, namun BUK UNP belum pernah melakukan perhitungan Analisis Beban Kerja (ABK) sebelumnya. ABK sendiri merupakan salah satu persyaratan dalam pengusulan formasi CPNS bagi instansi sehingga perhitungan ABK akan sangat berpengaruh terhadap jumlah formasi CPNS yang masuk untuk tiap instansi. Tujuan penelitian ini yaitu, (1) menganalisis jumlah beban kerja tendik PNS pada BUK UNP dan (2) menganalisis kebutuhan tendik PNS berdasarkan analisis beban kerja pada BUK UNP. Metode yang digunakan dalam penelitian ini yaitu metode analisis beban kerja menurut Permenpan RB Nomor 1 Tahun 2020 dan FTE. Metode nonprobability sampling dengan teknik purposive sampling dipakai sebagai penarikan sampel. Hasil perhitungan menunjukkan bahwa dibutuhkannya penambahan 8 orang serta pemindahan 3 orang pegawai ke unit lain pada Biro Umum dan Keuangan UNP dengan total pegawai optimal 5 orang.
Keyword: Analisis Beban Kerja, Analisis Jabatan, Analisis Kebutuhan Pegawai, Full Time Equivalent (FTE)