Judul: Pengaruh Interaksi Orang Tua-Anak dan Dukungan Sosial terhadap Insecurity Remaja Madya
Abstrak: Insecurity merupakan permasalahan yang marak terjadi pada remaja di Indonesia. Insecure merupakan pandangan kurang damai atau ketidaknyamanan yang dialami oleh individu. Karakteristik insecure yang sering terjadi adalah tidak percaya diri, kecemasan, khawatir terhadap masa depan, dan perasaan kurang dalam berbagai hal. Orang tua dan lingkungan sosial berperan penting dalam pencegahan insecurity pada remaja. Penelitian ini bertujuan untuk menganalisis pengaruh interaksi orang tua-anak dan dukungan sosial terhadap tingkat insecurity remaja madya. Penelitian ini menggunakan desain penelitian explanatory. Teknik penarikan sampel menggunakan metode non-probablity sampling dengan teknik voluntary sampling. Jumlah sampel sebanyak 202 responden siswa SMA berusia 16-18 tahun dan berdomisili di Jabodetabek. Hasil penelitian menunjukkan hubungan negatif antara pendidikan orang tua, interaksi orang tua-anak dan dukungan sosial dengan insecurity remaja. Terdapat pengaruh negatif signifikan antara pendidikan ibu, interaksi orang tua-anak dan dukungan sosial terhadap insecurity remaja. Berdasarkan penelitian ini interaksi orang tua-anak dan dukungan sosial yang baik akan menurunkan tingkat insecurity pada remaja., Insecurity is a problem that is currently happening among adolescent in Indonesia. Insecure is a lack of peace feeling or discomfort experienced by individuals. Insecure characteristics are lack of self confidence, anxiety, overthink about future, and feelings of lack in everything. Parents and social environment around adolescent play an important role in preventing insecurity. This study aims to analyze the effect of parent-child interaction and social support on insecurity among adolescent. This study used an explanatory research design. The sampling technique used a non-probablity sampling method with a voluntary sampling technique. The sample size was 202 high school student respondents aged 16-18 years in Jabodetabek. The results showed negative relationship between parent education, parent-child interaction and social support with adolescent insecurity. There was a significant negative effect between mother’s education, parent-child interaction and social support with adolescent insecurity. Based on this study, a good parent-child interaction and social support will reduce the level of insecurity in adolescents.
Keyword: adolescents, insecurity, parent-child interaction, social support

Judul: Kelekatan Remaja dengan Orang Tua dan Teman Sebaya, Penghargaan Diri, dan Online Resilience Remaja
Abstrak: Salah satu cara agar remaja dapat menghadapi risiko saat menggunakan internet adalah dengan memiliki online resilience yang baik. Online resilience merupakan kemampuan seseorang dalam menyikapi berbagai pengalaman ketika menggunakan media sosial. Penelitian ini bertujuan untuk menganalisis pengaruh karakteristik remaja, karakteristik keluarga, kelekatan orang tua, kelekatan teman sebaya, dan penghargaan diri terhadap online resilience remaja. Penelitian ini menggunakan desain eksplanatori. Responden dalam penelitian ini berjumlah 224 remaja yang berada pada rentang usia 15-18 tahun, memiliki dan tinggal dengan orang tua lengkap, serta menggunakan media sosial. Kota Bogor sebagai lokasi penelitian dipilih secara purposive, dengan pengambilan contoh sebanyak empat SMA yang terdiri atas dua sekolah negeri dan dua sekolah swasta dengan menggunakan simple random sampling. Data kemudian diolah dengan analisis deskriptif, uji korelasi, dan uji regresi linier berganda. Penelitian ini meneliti kelekatan orang tua dan remaja per dimensi. Dimensi kepercayaan keduanya terkategori sedang, dan dimensi komunikasi serta dimensi pengasingan terkategori rendah Lebih dari separuh remaja memiliki penghargaan diri dengan kategori rendah, dan online resilience kategori sedang. Hasil penelitian menunjukkan dimensi kepercayaan, komunikasi, dan pengasingan pada kelekatan ayah, kelekatan ibu, dan kelekatan remaja berhubungan nyata dengan penghargaan diri remaja. Selain itu, gender remaja dan dimensi pengasingan pada kelekatan ayah berpengaruh signifikan positif terhadap online resilience remaja., One of the things that teenagers should prepare for when facing risks on the internet is having good online resilience. Online resilience is the ability to respond to the experience that happened when using social media. This study aims to analyze the influence of parent attachment, peer attachment, and self-esteem on online resilience on adolescent social media users. This research used an explanatory design. A total of 224 adolescent social media users with the age of 15-18 also have and live with complete parents was participated in this study. Bogor City was chosen purposively as the location of this study, while the senior high school (two public schools and two private schools) was selected with simple random sampling. The data were analyzed with descriptive tests, correlation tests, and multiple linear regression tests. This study examined the attachment of parents and adolescents per dimension. Both trust dimension is categorized as a medium, and both the communication dimension and alienation dimension is in a low category. More than half of the adolescents have self-esteem in the low category and online resilience in the medium category. The result showed that trust, communication, and alienation dimensions in father, mother, and adolescent attachment has a significant correlation with adolescent’ self-esteem. The result also showed that gender and alienation dimensions on father attachment have positive significant effects with online resilience in adolescent social media users.
Keyword: adolescent, online resilience, parent attachment, peer attachment, self-esteem

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable