Judul: Pengaruh persepsi gaya pengasuhan orangtua dan konsep diri terhadap motivasi berprestasi atlet muda di SMA Negeri Ragunan Jakarta
Abstrak: Pencapaian prestasi atlet muda tidak terlepas dari pengaruh berbagai faktor, yaitu faktor fisik, teknik, dan psikologis. Faktor psikologis tersebut merupakan aspek yang penting dalam menunjang motivasi berprestasi dari seorang atlet. Dalam hal ini, penetapan sasaran dan persiapan mental menghadapi pertandingan akan memacu atlet untuk meningkatkan motivasinya, sehingga mampu meraih prestasi di bidang olahraga. Selain itu, konsep diri yang dimiliki oleh seorang atlet juga akan memberikan hasil pada penampilan atlet di arena pertandingan. Konsep diri merupakan hasil dari pola pengasuhan orangtua, dimana konsep diri tersebut diduga mempunyai pengaruh terhadap motivasi berprestasi.
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Judul: Sibling Rivalry, Komunikasi Orang tua-Remaja dan Motivasi Berprestasi.
Abstrak: Beberapa faktor yang memengaruhi motivasi beprestasi adalah dukungan keluarga, baik dari orang tua maupun saudara sekandung. Penelitian ini bertujuan untuk menganalisis pengaruh sibling rivalry, dan komunikasi orang tua-remaja terhadap motivasi berprestasi remaja laki-laki maupun perempuan. Desain yang digunakan dalam penelitian ini ialah cross-sectional study pada mahasiswa PPKU IPB. Contoh penelitian berjumlah 60 orang dengan kriteria mahasiswa bukan anak sulung, memilliki kakak kandung yang berada pada tahap perkembangan dewasa awal yaitu lebih dari 21 tahun dan memiliki orang tua lengkap. Teknik yang digunakan dalam pengambilan contoh menggunakan teknik simple random sampling. Hasil penelitian menunjukkan tidak ada perbedaan rata-rata indeks sibling rivalry, komunikasi orang tua-remaja dan motivasi berprestasi antara remaja laki-laki dan remaja perempuan. Komunikasi yang bermasalah antara orang tuaremaja dan dimensi rasa cemas berpengaruh negatif terhadap motivasi berprestasi, sedangkan urutan kelahiran remaja, pekerjaan ayah dan dimensi keterbukaan komunikasi orang tua-remaja berpengaruh positif terhadap motivasi berprestasi.
Keyword: komunikasi orang tua-remaja, motivasi berprestasi, remaja akhir, sibling rivalry

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable