Judul: Isolation of Endophytic Actinomycetes in Medicinal Plants and Their Potency as an Antidiabetes Based on α-glucosidase Activity.
Abstrak: Endophytic actimomycetes lives in plant tissues without causing a negative impact on plant. Several medicinal endophytic microbes are able to produce the same bioactive compounds as produced by the host. Inhibition of α-glucosidase is one of antidiabetes mechanism. The potency of endophytic actinomycetes from medicinal plant as α-glucosidase inhibitor needs to be elucidated. Temulawak (Curcuma xanthorrhiza), ciplukan (Physalis minima) and brotowali (Tinospora crispa) were surface sterilized and crushed, then plated on humic acid vitamin B. Actinomycetes colonies were purified and produced on International Streptomyces Project No. 4 followed by α-glucosidase assay. Crude extracts with the highest inhibition of α-glucosidase were freeze dried, then diluted at 0.07%, 0.1%, 0.3%, 0.5% (w/v) with dimethylsulfoxide and assayed. Commercial inhibitor, acarbose (Glucobay; Bayer) were used as a comparation. Fourty six isolates were classified into 29 isolates Gram positive and 17 isolates Gram negative. Fifteen isolates were actinomycetes which 11 isolates obtained from brotowali stem, and 4 isolates from temulawak rhizome. The highest inhibition (61.27%) were shown by Cx-10.1 temulawak isolate at 0.07% (w/v) concentration compared with Glucobay 0.1% (w/v) which was only 39.70%. At 0.07% (b/v) concentration, Tc-2.1 from brotowali produce low activity (24.16%). Endophytic Cx-10.1 isolate could be potential to produce higher activity of α-glucosidase inhibitor compared with Glucobay.
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Judul: Potensi Aktinobakteri Endofit Tabat Barito ('Ficus deltoidea Jack) sebagai Penghasil Inhibitor a-Glukosidase
Abstrak: Diabetes mellitus (DM) is a chronic and serious metabolic disorder that is t revalent in the world. Diabetes mellitus occurs due to insulin deficiency and inswli resistance. This condition causes high blood glucose levels or called hYN.. rglycemia condition. One therapeutic approach to treat diabetes is to retard the abs" tion of glucose via inhibition of enzymes, such as a-glucosidase, in the small tinal. Inhibition of this enzyme slows the elevation of blood glucose following · rl:hohydrate meal. The a-glucosidase inhibitor is a bioactive compounds i8uted widely in plants and microorganisms including actino bacteria. }\ctinobacteria are Gram positive bacteria widely distributed in nature. They produce numerous metabolic products, most of which are bioactive compound with applications in many therapeutic areas. Actinobacteria are predominantly free living and are found in diverse environments including plants tissue. Microbes that live on plant tissues without causing harmfull to the host are called endophytes. Such icrobial endophytes are beginning to pay attention ofresearchers due to their abilit)1 to produce various compounds of pharmacological value similar to their li.osts. Previous reports showed that endophytic actinobacteria have a-glucosidase mhibi or activity such as Tinospora crispa (Brotowali) and Datura stramonim L (Kecubung). lf abat barito (Ficus deltoidea Jack) is a medicinal plant originating from Southeast Asia. In Indonesia, tabat barito has the most distribution in Borneo island. Tabat barito was used traditionally to treat diabetes. On another hand, several studies have shown that tabat barito has an a-glucosidase inhibitor activity. The abilit)1of tabat barito to produce a-glucosidase inhibitors is suggested to be related to the presence of endophytic microbes including actinobacteria. Previous studies showed that the abundance of actinobacteria in the tabat barito plant tissue is quite umer:ous and varied. Based on these reports, it is expected that the capability of abat barito on producing a-glucosidase inhibitors is related to the presence of actinobacteria. In this study, it was proven that endophytic actinobacteria from tabat barito <t:ould produce a-glucosidase inhibitors. Screening using a-glucosidase from rat intestine is recommended for in vitro a-glucosidase inhibitor because the homology sequeroce of catalytic side closer to enzymes in the human intestine. In this study endophytic actinobacterial isolates more inhibited a-glucosidase from rat intestines according to acarbose which only inhibit mammalian a-glucosidase. Screening of 40 enoophytic actinobacteria isolates using yeast a-glucosidase showed that 25 sG).}ates (62%) of isolates had a-glucosidase inhibitors activity. Whereas screening msm rat intestinal enzymes from 40 isolates, 31 isolates (77%) had a-glucosidase lnor activity. Aqueous extract and n-hexane ofTBL 7 isolates were the best extracts for a­ sidase inhibition, IC₅₀ value of 159.25 µg mL-¹ (aqueous extract) and 118.52 mIL-1 (n-hexane extract). Based on the 16S rRNA gene TBL 7 showed was similar to Streptomyces tendae (99.91%). The ISP 2 medium with 10 days incubation period is the best condition to produce a-glucosidase inhibitor compound. After fractionation, AF 2 from aqueous crude extract has ICso value of 58.8 µg mL-I, higher than acarbose as positive control (ICso = 90.4 µg mL-I). The LC-MS analysis showed that the fraction of AF 2 had bioactive compounds similar t phenylpropynal, butyric acid, solketal, p-coumaric
Keyword: Actinobacteria, Diabetes mellitus, Endophytes, Tabat barito

Judul: Transisi Fasa Materi Hadronik ke Materi Kuark pada Inti Bintang Neutron.
Abstrak: Bintang neutron terdiri dari beberapa lapisan yang disusun oleh materi nuklir yang berbeda dengan kerapatan yang semakin tinggi ketika mendekati pusat bintang. Inti luar bintang neutron disusun oleh materi hadronik (nukleon, lepton, dan hyperon), sedangkan inti dalam bintang neutron disusun oleh kuark-kuark bebas (up, down, strange) yang saling berinteraksi. Tujuan penelitian ini adalah mempelajari bagaimana transisi dari inti luar ke inti dalam bintang neutron dengan menggunakan pendekatan RMF (Relativistic Mean Field) untuk materi hadronik dan CIDDM (Confined Isospin Density Dependent Mass) untuk materi kuark. Tekanan transisi diperoleh ketika potensial kimia barion materi hadronik dan materi kuark bernilai sama. Nilai tekanan transisi dengan parameter SU6-TM1 sekitar 87 MeV/fm3 dan diperoleh massa maksimum bintang neutron yaitu 1.92M dengan jari-jari sekitar 8.62 km. Nilai tekanan transisi dengan parameter SU6-G2 sekitar 53 MeV/fm3 dan massa maksimum bintang neutron yaitu 1.88M dengan jari-jari sekitar 8.16 km. Jenis transisi fasa adalah orde satu ditandai dengan diskontinuitas dalam kerapatan energi (adanya mixed phase). Parameter-parameter pada model nuklir yang digunakan menyebabkan perbedaan kekakuan persamaan keadaan (EOS) dari materi hadronik dan materi kuark sehingga nilai tekanan transisi dari fasa hadronik ke fasa kuark juga berbeda.
Keyword: bintang neutron, persamaan keadaan, transisi fasa