Judul: Pengetahuan dan Sikap Gizi Ibu, Pola Asuh, dan Pertumbuhan Linier Baduta di Rumah Susun Kemayoran Jakarta.
Abstrak: Stunting merupakan salah satu bentuk malnutrisi yang merefkleksikan kekurangan gizi kronis sehingga balita mengalami keterlambatan pertumbuhan linier. Penelitian ini bertujuan menganalisis hubungan pengetahuan dan sikap gizi ibu, pola asuh, dan pertumbuhan linier baduta di Rumah Susun Kemayoran Jakarta dengan desain studi crossectional melibatkan 55 baduta. Hasil penelitian menunjukkan bahwa tingkat pengetahuan gizi dan sikap gizi ibu sebagian besar tergolong baik. Data dikumpulkan melalui wawancara dan antropometri. Pola asuh makan terkategori sedang sedangkan pola asuh kesehatan terkategori baik. Tingkat kecukupan energi, protein, dan zinc sebagian besar subjek berada dalam kategori cukup, sedangkan tingkat kecukupan kalsium tergolong kurang. Sebagian besar subjek memiliki pertumbuhan linier berdasarkan status gizi PB/U yang terkategori normal. Berdasarkan uji korelasi Spearman terdapat hubungan signifikan antara tingkat pendidikan ibu dengan pengetahuan gizi ibu, PB lahir dan BB lahir dengan pertumbuhan linier, pengetahuan gizi ibu dengan pertumbuhan linier dan sikap gizi ibu dengan pola asuh kesehatan. Tidak terdapat hubungan signifikan antara sikap gizi ibu dan pola asuh dengan pertumbuhan linier, pengetahuan gizi ibu dengan pola asuh, dan sikap gizi ibu dengan pola asuh makan.
Keyword: pengetahuan gizi, pertumbuhan linier, pola asuh, sikap gizi, stunting

Judul: Pengetahuan, Sikap, dan Praktik Ibu Balita Stunting Usia 24−59 Bulan di Kecamatan Cibungbulang, Kabupaten Bogor
Abstrak: Stunting merupakan salah satu permasalahan gizi kronis di Indonesia yaitu kondisi kurangnya panjang atau tinggi badan balita menurut usianya yang berkaitan dengan pengetahuan, sikap, dan praktik ibu mengenai stunting. Penelitian ini bertujuan untuk mengetahui pengetahuan, sikap, dan praktik ibu mengenai stunting pada balita usia 24−59 bulan. Desain penelitian ini adalah cross sectional study yang dilaksanakan pada bulan November hingga Desember 2019 di Kecamatan Cibungbulang, Kabupaten Bogor. Subjek berjumlah 86 orang ibu yang memiliki balita stunting yang dipilih dengan metode purposive sampling. Hasil penelitian menunjukkan bahwa subjek memiliki pengetahuan dan sikap positif yang kurang mengenai stunting, yaitu 53,5% dan 66,3%. Namun, sebagian besar subjek memiliki praktik yang baik mengenai stunting (91,9%). Terdapat hubungan yang signifikan antara tingkat pendidikan ibu dengan pengetahuan ibu mengenai stunting (p=0,014). Hubungan antara pengetahuan dan sikap ibu mengenai stunting juga menunjukkan hasil yang signifikan berdasarkan uji korelasi Spaerman (p=0,000; r=0,617)., Stunting is one of chronic nutritional problems in Indonesia that is condition of the lack of length or height of toddlers according to their age related to the knowledge, attitude, and practice of mothers regarding stunting. The aim of this study is to determine the knowledge, attitude, and practice of mothers regarding stunting in toddlers aged 24−59 months. This study used a cross sectional study design which was conducted in November until December 2019 in Cibungbulang District, Bogor Regency. The subjects were 86 mothers with stunting toddlers who was selected by purposive sampling method. The results showed that the subjects had poor knowledge and positive attitude regarding stunting, i.e 53.5% and 66.3% respectively. However, most of the subjects had good practice regarding stunting (91.9%). There is a significant relationship between maternal education level and knowledge regarding stunting (p=0.014). The relationship between maternal knowledge and attitude regarding stunting also showed significant results based on the Spearman correlation test (p=0.000; r=0.617).
Keyword: attitude, knowledge, practice, stunting toddler

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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