Judul: Pembentukan rimpang mikro secara in vitro dan analisis kestabilan genetik antar sub-kultur  temulawak (Curcuma xanthorrhiza Roxb.)
Abstrak: Temulawak (Curcuma xanthorrhiza Roxb.) was used to be propagated from rhizome. High propagul weight will produce high yield. The use of big sized rhizome for seedling will reduce its use for consumption and processing purposes. Therefore, in vitro micro rhizome could be used as propagul alternative for the propagation of temulawak because of its simplicity and high multiplication in shorter time. Plantlet from repeated sub-cultures induced to form micro rhizome. High rate shoot multiplication caused somaclonal variation. In plants propagation, somaclonal variation was not desired because seed uniformity was very important. Therefore, genetic stability of plantlets for get true to type seed from the use of repeated sub-culture in vitro shoot for micro rhizome formation must be studied. This research used RFLP and sequencing method to find the genetic stability of plantlet among sub-cultures generations. RFLP method was used to mother plant’s DNA and plantlet’s DNA from 3rd, 4th, 5th and 12th sub-cultures. Sequencing analysis was used to mother plant’s and 12th sub-cultures plantlet DNA. In vitro micro rhizome formation research consisted of three studies : 1) The effect of physical of media (liquid and solid medium) and sucrose (30, 60, 90, 120 gL-1); 2) The effect of the strength of MS medium (half and full strength) and sucrose (30, 60, 90, 120 gL-1) ; 3) The effect of level of BAP (0, 1, 2, 3 mgL-1) and sucrose (30, 60, 90, 120 gL-1) to plantlet growth and micro rhizome formation. Cultures were incubated in the dark for 16 hours day-1. Electrophoresis band of DNA temulawak in RFLP analysis with restriction enzymes MBo1, Taq1 and Alu1 showed the same pattern between 5 samples DNA. There is possibility of genetic stability of temulawak plantlet between generation 3rd, 4th, 5th, and 12th sub-cultures and mother’s plants DNA. Homology result of sequencing DNA also showed genetic stability for 300 bp DNA. There is genetic sequence equality between DNA mother plants sequence at base number 61-361 and DNA plantlet 12th sub-culture at base sequence number 61- 361. Micro rhizomes of temulawak was formed in liquid half and full strength MS. Interaction BAP and sucrose have significant effect on rhizomes formation at 18 weeks after planting. The higher frequency of rhizomes formation was enhanced in medium with BAP 1 and 2 mgL-1 on sucrose concentration 60, 90, 120 gL-1. The increased of sucrose concentration also had positive effect to higher number, diameter and weight of micro rhizomes 8 weeks after acclimatization.
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Judul: Pola Keragaman Genetik Tanaman Kunyit (Curcuma longa Linn.) dan Temulawak (Curcuma xanthorrhiza Roxb.) Asal Pulau Jawa dengan PCR-RAPD
Abstrak: Turmeric (Curcuma longa Linn.) and Temulawak (Curcuma xanthorrhiza Roxb.) are Indonesian medicinal plants which nutritious for improving health. The pattern of genetic diversity and genetic relationship of these rhizome plants are not well known yet. The purpose of this research to analyze the pattern of genetic diversity and genetic relationship both plants using Polymerase Chain Reaction- Random Amplified Polymorhic DNA (PCR-RAPD). The sample used in this research include 6 turmeric plants from Bogor, Wonogiri, Karanganyar, Sukabumi and Ngawi; and 5 temulawak plants from Bogor, Wonogiri, Sragen, Karanganyar and Sukabumi. Stage of these research include DNA isolation; DNA amplification; and analysis pattern of genetic diversity and genetic relationship using NTSYS 2.02. The result of these research showed that isolation DNA was done successfully. The result of DNA isolation generally has a fairly good purity. The purity values of turmeric and temulawak in sequence were 1.093-1.417 and 1.044-1.544. The number of bands that arise from DNA amplification of turmeric and temulawak in sequence were 131 and 132 locus. The number of amplification bands mixed results and different sizes for each sample show the patterns of genetic diversity found in turmeric and temulawak plants. The closest genetic relationship showed by turmeric plants from Sukabumi and Ngawi; and temulawak plants from Bogor and Sukabumi. Genetic similarity coefficient ranged from turmeric plants and temulawak plants in sequence are 54%-92% and 55 %-83 %.
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Judul: Orbit elektron dan probabilitas level energi sistem chaotic atom hidrogen
Abstrak: a. Mendapatkan orbit elektron sistem chaotic atom hidrogen (dalam koordinat semiparabolik). b. Mendapatkan probabilitas dua level energi yang berdekatan dari sistem chaotic atom hidrogen.
Keyword: Atom, Orbit Elektron