Judul: Penentuan pH dan suhu optimum aktivitas kitinase Bacillus cereus I.5 dan pengujian kitinase dalam mendegradasi eksoskeleton kutu bertepung putih (Ferrisia virgata Cockerell)
Abstrak: Bacillus cereus I.5 is one of chitinolytic bacteria isolated from chilli rhizosphere. The chitinase could be apply as a biocontrol of plant pest. One method to precipitate enzyme by using ammonium sulphate. It could increase specific activity enzyme. This research was conducted to determine optimum pH and temperature of chitinase activity of Bacillus cereus I.5 and tested its ability to degrade chitin exoskeleton of Ferrisia virgata Cockerell, one of Jatropha’s pest. Enzyme activity was obtained at the 10% ammonium sulphate saturation level, which is 20 times higher compared to crude enzyme. Specific enzyme activity increased from 0,039 U/mg of crude extract to 0,778 U/mg after ammonium sulphate precipitation. Chitinase of B. cereus i.5 showed highest activity at pH 7,0 and 55 °C although the enzyme also showed its activity at pH 5,0-9,0 and temperature 26-≤55 oC. Application of crude chitinase and the enzyme after ammonium sulphate precipitation on degrading of chitin Ferrisia virgata showed it worked better if incubated firstly at its optimum temperature.
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Judul: Karakterisasi kitinase hasil pemurnian parsial dari isolat bakteri tanah asal Cianjur
Abstrak: Kitinase (EC 3.2.1.14) merupakan enzim yang mampu menghidrolisis kitin yang terdapat dalam eksoskeleton serangga, bakteri, dan jamur, serta enzim ini berpotensi sebagai agen biokontrol. Tujuan penelitian ini adalah memurnikan dan mengkarakterisasi kitinase dari isolat terpilih, serta mengukur kemampuan penghambatan terhadap jamur Colletotrichum sp. oleh bakteri kitinolitik. Aktivitas kitinase ditentukan dengan metode Spindler dan kadar proteinnya ditentukan dengan metode Bradford. Produksi kitinase isolat rizobakteri menunjukkan aktivitas spesifik kitinase tertinggi sebesar 0.0489 U/mg pada isolat C5C dengan pemurnian amonium sulfat 70% serta dapat meningkatkan kemurnian 13.97 kali dibandingkan ekstrak kasar dan fraksi lainnya. Karakterisasi kitinase asal isolat C5C menunjukkan bahwa enzim aktif pada suhu 55 °C, pH 7, waktu inkubasi optimum 120 menit, serta enzim ini memiliki nilai Km sebesar 1.300x 103 mg/L dan Vmaks sebesar 0.0294 mg L -1 detik-1 . Aktivitas antifungi isolat bakteri kitinolitik C5C dapat menghambat pertumbuhan jamur Colletotrichum sp. secara in vitro.
Keyword: Biocontrol, Chitin, Chitinase, Colletotrichum sp., Rhizobacteria, Insect, Bacteria, Fungi, Biocontrol agent, Chitinolitic bacteria, In vitro

Judul: Visualisasi Data Spasial di MonetDB Menggunakan OpenJUMP
Abstrak: There are currently the most used data model is relational. This model a database as a set of data that is stored in structural rows and columns. Besides the popullarity, the relational data model is lacking in the implementation of data is large and has a high complexity. Different of the relational model, MonetDB using a column store method to divide the data into sections binary tables . This method is only necessary to call the data, resulting method is faster. Anggi ( 2013 ) have tested the MonetDB as a spatial and non spatial data storage, but not yet visualize data from MonetDB. This result successfully visualize spatial data using a simple query “like” and “and” the average time 1 second , while the complex spatial queries using “joins”, “constains” and “Intersect” with average time more than 20 minutes. So it looks that MonetDB also can process spatial data simple and complex . But in the process of complex spatial data visualization that require run-time is relatively long .
Keyword: visualize, OpenJUMP, MonetDB, spatial data