Judul: Identification Shark Fin Morphologically and Biomolecular with Partial COI Gene
Abstrak: Ikan hiu memiliki permintaan pasar yang tinggi khususnya terhadap bagian siripnya. Faktor tersebut memicu terjadinya IUU (Illegal, Unreported and Unregulated) fishing terhadap hewan Kelas Elasmobranchii ini. Beberapa jenis hiu di Indonesia telah masuk ke daftar merah IUCN. Identifikasi terhadap hiu sulit dilakukan karena hiu dijual tidak dalam keadaan utuh dan memiliki kemiripan secara morfologi, umumnya dalam bentuk sirip. Penelitian ini bertujuan mengidentifikasi jenis sirip ikan hiu yang didapatkan di Muara Baru, Jakarta. Proses analisis meliputi identifikasi morfologi dan molekuler dengan teknik DNA barcoding menggunakan marka gen parsial COI. Jumlah sampel pada penelitian ini sebanyak 4 buah sirip hiu yang berbeda secara morfologis. Pada penelitian ini sampel sirip ikan hiu kode SP281 secara morfologis tidak dapat dipastikan, tetapi secara molekuler dapat dipastikan memiliki kemiripan dengan Sphyrna lewini dengan nilai identify sebesar 99,85%. Penelitian ini berhasil mengidentifikasi 4 spesies yang berbeda, yaitu Carcharhinus albimarginatus, Carcharhinus amblyrhynchoides, Sphyrna lewini, dan Prionace glauca. Berdasarkan penelitian ini DNA barcode dapat mempermudah proses identifikasi dan mencegah kesalahan identifikasi. Aplikasi dalam pengelolaan adalah membantu melindungi jenis ikan hiu yang termasuk ke dalam daftar merah IUCN lolos dari perairan Indonesia dan siripnya diekspor keluar negeri. Kata kunci: Morfologi, COI, sirip hiu, konservasi
Keyword: COI, conservation, morphological, shark fin

Judul: DNA Barcoding dan Analisis Filogenetik Ikan Hiu yang Didaratkan di Pelabuhan Perikanan Samudera Cilacap
Abstrak: Indonesian waters are known to be lived by more than 75 species of shark. Most shark species are now being caught and traded for their body parts, including their fins, liver oil, meat, bones, skin, and eyes. However, trade data on shark in Indonesia is very lack. The identification of a shark fin to species level is very problematic, as morphologically similar species and specimens, which are poorly preserved or have had key diagnostic features removed, can be difficult to identify. Therefore this research was conducted to identify shark species being traded in Cilacap fisheries port, to confirm their conservation status with IUCN redlist and to determine their phylogenetic relationship. The PCR using COI (Cytochrome Oxidase I) was successfully amplified for 32 samples. A total of 10 spesies was identified and 6 clusters of different shark family was revealed. These identified shark were categorized in three IUCN conservation status : vulnerable status (Sphyrna zygaena, Alopias superciliosus, Carcharhinus obscurus, Isurus paucus, and Isurus oxyrinchus), near threatened status (Pseudocarcharias kamoharai, Carcharhinus falciformis, Prionace glauca, and Squalus hemipinnis) and one species in endangered status (Sphyrna lewini). The identification result is important to help government to build shark conservation and trading policy.
Keyword: PPS Cilacap, conservation, species identification, shark, phylogenetic, DNA Barcoding

Judul: Pengaruh Faktor Pembentuk Agregat Tanah terhadap Kemantapan Agregat Tanah Latosol Dramaga pada Berbagai Penggunaan Lahan
Abstrak: Latosol Soil generally used for agricultural activities. Soil aggregate stability is an important property because it affects to many other soil physical properties. Soil aggregate stability is influenced by various factors of foundation soil aggregate such as soil texture, clay mineral type, exchangeable cations, soil organic matter, biological processes, land use and soil tillage. The purpose of this research is to identify the influence of land use of soil aggregate stability and soil factors that influence on soil aggregate stability in Latosol Soil Dramaga. Analyzed parameters were soil aggregate stability, soil texture, soil organic matter, exchangeable calcium, magnecium, sodium. The analysis showed soil aggregate stability on all land uses were classified to very stable category (ISA> 200). Soil aggregate stabiliy was influenced by land use (P< 0.05). Highest soil aggregate stability was founded in Cocoa Garden and lowest in the Cassava Garden. Land use affected the Latosol Soil aggregates stability and aggregateforming factors such as soil organic matter and exchangeable calcium (P< 0.05). Analysis of variance showed soil aggregate stability were significantly influenced by percent of soil organic matter, exchangeable calcium, magnecium and sodium, and percent of clay and sand (R-Square = 93%, P< 0.05). The exchangeable calcium and magnecium had the highest impact than the other aggregate form factor in soil aggregates stability (P = 0.004).
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