Judul: Transformasi Genetik Tanaman Tembakau oleh Gen LFY Kakao (TcLFY) Menggunakan Agrobacterium tumefaciens
Abstrak: LFY is a protein that have important role in flowering. TcLFY gene have been isolated from cacao (Theobroma cacao L.). To examine its role, this gene that is under control of p35S CaMV promoter was introduced in the tobacco plant using Agrobacterium tumefaciens. Transformation efficiency of transgenic plants was 16 % with regeneration efficiency was 27.38%, and produce 4 buds each explant. PCR analyses toward 5 putative transgenic tobacco plants that were taken randomly showed that all of them were transgenic. They also flowered early. Expression analyse of TcLFY gene using PCR showed that transgenic plant expressed the TcLFY gene in the all of its parts. This result showed that transgenic tobacco plant contained TcLFY gene under control of p35S CaMV
Keyword: 

Judul: Penyisipan Gen LEAFY Lengkap Tanaman Kakao pada Vektor Ekspresi dan Transformasi ke dalam Agrobacterium sp.
Abstrak: Cacao (Theobroma cacao L.) is one of the plantations that has important role for the national economy. However, one of the problems faced in the cocoa plantations is cherelle wilt. Cherelle wilt reduces the level of productivity of cocoa. Flowering genes regulate the normal flower formation in cacao. One of the genes that play a major role in the flower formation is the LEAFY gene. Studying the flowering genes are expected to elucidate the effect of these genes to cherelle wilt. The objective of this research is to construct expression vector harbouring fulllength of LEAFY gene. The fulllength of LEAFY gene that has been inserted into the expression vector was then transformed into Agrobacterium tumefaciens AGL-0. Cloning of LEAFY gene into expression vector was carried out using the Gateway technology. The full size LEAFY gene of 1200 bp was first inserted into a donor vector and then inserted into the expression vector. Characterization of expression vector inserted with LEAFY gene was carried out using the PCR plasmid technique which resulted in a DNA band of 500 bp. Expression vector inserted with LEAFY gene was transformed into the Agrobacterium tumefaciens AGL-0. Analysis of recombinant plasmid after transformation into the Agrobacterium tumefaciens AGL-0 using PCR plasmid technique result in a DNA band of 500 bp. The conclusion of this research is that the fulllength of LEAFY gene had successfully been inserted into the expression vector with Gateway technology.
Keyword: 

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
Keyword: