Judul: Perkembangan Embrio Sapi setelah Fertilisasi Menggunakan Metode Intracytoplasmic Sperm Injection (ICSI) dan Aktivasi dengan Strontium
Abstrak: Intracytoplasmic Sperm Injection (ICSI) is an in vitro fertilization method by injecting single sperm into oocyte cytoplasmic. The mechanic treatment on ICSI causes the sperm less optimal in oocytes activation. The oocyte activation after ICSI using strontium has a purpose to improve in vitro development of bovine embryo. The first experiment was conducted to find out the development of parthenogenetic embryo result using different strontium concentration for 6 hours. The second experiment was conducted to find out the influence of combination ICSI and strontium activation in order to find out pronucleous development. Combination ICSI and 20 mM of strontium increased development to pronucleous stage 2-PN up to 43,59%. The third experiment is to evaluate the in vitro development of embryo after ICSI followed by strontium activation. The development of embryo after ICSI treatment and 20 mM strontium up to 2-4 cells was 50,5%, 8-16 cells was 43,73%, and blastosyst was 15,63%. The conclusion of the experiments is the fertilization by ICSI method followed by activation using strontium 20 mM gives better results of bovine in vitro embryo development.
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Judul: Pembentukan Blastosis Pada Embrio Sapi yang Difertilisasi Secara In Vitro dengan Semen Sapi Bali (Bos javanicus) dan Semen Sapi Simmental (Bos taurus)
Abstrak: In vitro fertilization is one of reproductive biotechnology which can be used to increase population, productivity, and quality of cattle in Indonesia. Bali cattle (Bos javanicus) is one of native cattle from Indonesia, which have good carcass, meat production, which has adapted with invironment, and also have good fertility. Development of Bali cattle population can be accived through Embryo Transfer (ET) using IVF embryo production. The aim of this study was to determine the ability of Bali cattle semen (Bos javanicus) and compared with Simmental cattle semen (Bos taurus) to fertilized and its effect on cleavage rate until the blastocyst stage. This study was conducted in May and June 2012 at laboratory IVF production of Balai Embrio Ternak Cipelang, Bogor. Matured oocytes will be fertilized using Bali cattle semen and Simmental cattle semen, which have been capacitated before. Comparisons between bulls for cleavage, morula, and blastocyst rate, were analyzed using Chi-square analysis. The results indicate that Bali cattle can produced higher cleavage rate, morula rate, and blastocyst rate compared with Simmental cattle for in vitro embryo production. Embryo cleavage rate, morula rate and blastocyst rate were significantly different (P>0,05). The competence of embryo development from the blastocyst rate at day 6 was significantly different (P<0,05) but blastocyst rate at day 7, 8, and 9 were not significantly different (P>0,05).
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Judul: Cycle Hamilton pada Graf Lengkap, Graf Regular, dan Graf 2-Connected 4-Regular Berorder Kurang dari Sepuluh
Abstrak: Suatu graf disebut graf Hamilton apabila graf tersebut memuat cycle Hamilton, yaitu cycle yang melewati setiap vertex tepat satu kali. Menentukan keberadaan cycle Hamilton pada suatu graf dapat menggunakan syarat cukup yang berupa teorema-teorema yang telah diperkenalkan sejak tahun 1950-an. Beberapa syarat cukup yang dibahas dalam karya ilmiah ini adalah syarat cukup yang berhubungan dengan graf lengkap dan graf regular. Suatu graf lengkap berorder 𝑛������ ≥ 3 dan graf r-regular berorder 𝑛������ ≤ 2𝑟������ + 1 merupakan graf Hamilton, Karena kedua graf tersebut memuat cycle Hamilton. Selanjutnya, syarat cukup tersebut digunakan untuk menentukan keberadaan cycle Hamilton pada graf 2-connected 4- regular berorder kurang dari 10.
Keyword: graf Hamilton, graf regular, graf lengkap, graf 2-connected 4-regular