Judul: Kemampuan Benih Kedelai (Glycine max L.) untuk Mempertahankan Viabilitasnya setelah Didera dengan Etanol
Abstrak: Seed lots with different viability levels, 60–80%, are needed for seed invigoration studies to improve seed vigor and crop yield. Laboratory experiments were conducted to determine an accelerated aging method with liquid ethanol (96%) producing the desirable seed-viability levels and the storability of the treated seeds on soybean (Glycine max L.). The experiments were conducted in the Seed Science and Technology Laboratory, Bogor Agricultural University from November 2012 to May 2013. Soybean seeds of varieties Gema, Burangrang, and Ijen were treated by different duration of soaking into 96% liquid ethanol. Viability of the treated seeds were tested with UKD-dp method. The seeds with desirable viability levels P20 and P40 , were stored in two storage conditions, 27–30°C with 61–72% RH and 22–27°C with 58–74% RH. The results indicated that soybean seed of varieties Gema, Burangrang, and Ijen, with viability level of 80% (P20 ) could be obtain by soaking the seed into ethanol 96% for 22 minutes 31.8 secons, 22 minutes 58.8 secons, 15 hours 19.8 minutes respectively, whereas those with viability level of 60% (P40 )were obtained by soaking durations of 99 minutes 27.6 secons, 109 minutes 34.2 secons, 40 hours 4.8 minutes, respectively. The seed with selected viability levels, (P20 ) and (P40 ), could maintain the viability for 8 weeks in the both storage conditions.
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Judul: Soybean (Glycine max (L.) Merrill) Seed Longevity Prediction Using Several Vigour Test Methods
Abstrak: Kedelai (Glycine max L.) merupakan salah satu komoditas penting di Indonesia. Metode yang tepat untuk menduga viabilitas berdasarkan nilai daya berkecambah setelah melewati periode tertentu dalam penyimpanan sangat penting untuk memastikan benih kedelai yang sampai ke tangan konsumen (petani) masih memiliki mutu yang baik. Penelitian ini bertujuan mendapatkan satu metode yang mampu menduga daya simpan benih kedelai. Penelitian dilaksanakan di Laboratorium Ilmu dan Teknologi Benih IPB. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) terdiri dari faktor lot benih yang tersarang pada periode simpan. Faktor pertama adalah lot benih kedelai yang terdiri atas 6 taraf yaitu Dena 1 (tahun 2019), Devon 1 (tahun 2019), Detap 1 (tahun 2019), Anjasmoro (tahun 2019), Gepak Kuning (tahun 2019), dan Argomulyo (tahun 2019). Faktor kedua adalah periode simpan dengan 7 taraf yaitu 0, 1, 2, 3, 4, 5 dan 6 bulan. Benih kedelai dari berbagai varietas yang digunakan sebagai lot benih memiliki daya berkecambah awal berkisar 84,25% - 94,25%, dapat mempertahankan viabilitasnya hingga 73,50% - 79,50% setelah disimpan selama 6 bulan. Kondisi kadar air sebelum penyimpanan berkisar 8,51% - 9,94% dan 8,41% - 9,51% pada 6 bulan penyimpanan. Kondisi suhu dan RH ruang penyimpanan berkisar 20,2 ± 2 ºC dan 49 ± 2% dengan kemasan benih berupa plastik polypropylene (PP) 0,8 mm. Ekstrapolasi pada grafik penurunan daya berkecambah menunjukkan benih kedelai yang diuji memiliki variasi daya simpan antara 8,5 – 11,8 bulan untuk penurunan hingga daya berkecambah 65% atau 7,0 – 8,9 bulan untuk penurunan hingga daya berkecambah 70%. Radicle emergence (RE) yang diamati pada 42 jam merupakan tolok ukur yang paling potensial untuk menduga daya simpan benih kedelai dengan nilai koefisien korelasi sebesar 0,6033 untuk menduga daya simpan hingga daya berkecambah menjadi 65% dan 0,6442 untuk menduga daya simpan hingga daya berkecambah menjadi 70%., Soybean (Glycine max L.) is one of the important commodities in Indonesia. The right method to estimate viability based on the value of germination after a certain period of storage is very important to ensure that soybean seeds that reach consumers (farmers) still have good quality. This study aims to obtain a method that can estimate the seed longevity of soybean. The research was carried out at the Seed Science and Technology Laboratory of IPB. This study used a completely randomized design (CRD) consisting of several nested seed lots in the storage period. The first factor is the soybean seed lot which consists of 6 levels, namely Dena 1 (in 2019), Devon 1 (in 2019), Detap 1 (in 2019), Anjasmoro (in 2019), Gepak Kuning (in 2019), and Argomulyo (in 2019). The second factor is the storage period with 7 levels, namely 0, 1, 2, 3, 4, 5, and 6 months. Soybean seeds of various varieties used as seed lots have initial germination rates ranging from 84.25% - 94.25%, can maintain their viability up to 73.50% - 79.50% after being stored for 6 months. Conditions of water content before storage ranged from 8.51% - 9.94% and 8.41% - 9.51% at 6 months of storage. The temperature and RH conditions of the storage room ranged from 20.2 ± 2 ºC and 49 ± 2%
Keyword: expired date, radicle emergence, rapid aging, storage, viability

Judul: Studi Taksonomi Katak Subgenus Papurana (Lesson, 1831) dari Halmahera (Anura, Ranidae, Hylarana), Studi Taksonomi Katak Subgenus Papurana (Lesson, 1831) dari Halmahera (Anura, Ranidae, Hylarana
Abstrak: I reevaluated taxonomic of subgenus Papurana position from Halmahera based on morphological and molecular analyses. My results show that two separate species occur on the Halmahera Island, each nested within two distinct clades (hereafter papua clade and celebensis clade), one of which corresponds to H. moluccana (celebensis clade) and the other to an unidentified species (papua clade). By p-distance ranging from 6.9 to 11.5% on the 16S rRNA gene, the unidentified species (Hylarana sp.) is genetically different from all congeners of the papua clade. The Hylarana sp. can be distinguished from all congeners by following combination of charactes: vomer teeth in two oblique rows with narrow inter-vomer distance; snout rounded dorsally; dorsum with few scattered cone-shaped tubercles that are black with white tips; distinct skin folds (ridges) on the dorsal side of the thigh coinciding with dark brown cross bars; a marbled pattern on the ventral side of the thigh and yellowish groin. I also present new data for H. daemeli, H. volkerjane, and H. arfaki from the western region of Papua. Furthermore, I found that H. celebensis has numerous mtDNA lineages, indicating cryptic diversity within the celebensis clade. The discovery of two separate species on Halmahera necessitates more research into the biogeographic history of Hylarana in Northern Moluccas.
Keyword: frog, Halmahera, Hylarana, morphology, Papurana, phylogenetic