Judul: Induksi Proliferasi Tunas Adventif Kenaf (Hibiscus cannabinus L.) Secara In Vitro Melalui Penambahan Zat Pengatur Tumbuh 2.4-D Dan BAP
Abstrak: This research aimed to study the response of plant growth hormones 2.4-D and BAP to stimulate shoot proliferation initiation from three type of explants (meristem, meristem with cotyledon, and cotyledon) of kenaf. The research performed at Tissue Culture Research Laboratory, Agronomy and Horticulture Department, Agriculture Faculty, Bogor Agriculture University, from February to November 2010. Adventitious shoot formation on MS media supplemented with combinations of 2,4-D (0.0, 0.5, 1.0 mg/l) and BAP (0.0, 2.0, 4.0, 8.0, 10.0 mg/l). Callus began to appear in 2 weeks for all treatments on meristem and meristem with cotyledon. Results of this research showed that the medium composition without the 2.4-D + BAP had the highest number of adventitious shoots from meristem with cotyledon explants. Adventitious shoots formed after 11 weeks in culture without callus formation. The highest number of adventitious shoots resulted from the medium composition without 2.4-D +10.0 mg/l BAP. The medium composition without the 2.4-D +4.0 mg / l BAP resulted the highest number of roots from cotyledon explants. The roots of kenaf formed after 3 weeks which is marked by the emergence of roots from callus that formed on cotyledon explants.
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Judul: In Vitro Shoot Induction of Kaempferia parviflora Wall. Ex. Baker
Abstrak: This research aimed to find the best MS salt concentration and cytokinin (BAP) concentration for K. parviflora rapid multiplication by using in vitro buds as explants. The experiment was done at Plant Tissue Culture Laboratory of Agronomy and Horticulture Department, Faculty of Agriculture, Bogor Agricultural University from January to November 2011. This research began by sterilizing shoots of K. parviflora rhizomes received from PT. Ogawa Indonesia to produce axenic culture. After about 8 weeks of growth, young shoots began to sprout. The young shoots of K. parviflora axenic culture were then cut and cultured individually on the treatment medium, which consist of two different MS salt concentrations (MS and ½ MS) and five different 6-benzylaminopurine (BAP) concentrations (0, 1.25, 2.5, 3.75, and 5 ppm) respectively. Explants started to grow after 2 weeks of culture. Both MS salt concentration and BAP concentration treatments did not give a significant effect on the first shoot induction time, but ½ MS medium supplemented with 5 ppm BAP could induce first shoot faster than other treatments, which is within an average of 2.5 weeks after cultured (WAC). At early observation (2 to 4 WAC), medium enriched with 3.75 ppm BAP induced more shoot than the other treatments, but at the end, control medium (MS0) induced more shoot than other treatments. Starting from 2 WAC, the explants cultured on 0 ppm BAP medium grow more rapidly than the other treatments, but after 10 WAC, they grow slower. All treatments have the same trend of leaf growth. At 2 to 8 WAC, the number of leaves is rising rapidly, but at the 9 WAC, it began to slow down.
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Judul: Sirkuit Euler dan Penentuan Rute Optimal
Abstrak: Chinese Postman Problem (CPP) is well-known as a problem of determining the optimal route. In this work, the problem of CPP is studied by using two algorithms, namely Fleury algorithm and van Aardenne-Ehrenfest - de Bruijn algorithm. The solution of CPP can then be stated as an optimal route, which satisfies a condition, where every single edge must be traversed exactly once, i.e., Eulerian circuit. Graph balancing process in determining Eulerian circuit is done by using Orloff transportation method. The determination of extra shortest path is solved using Dijkstra algorithm. We discuss the application of determining Eulerian circuit for the case of determining the shortest route of garbage collection.
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