Judul: Molecular identification of the indian mackerel (Rastrelliger kanagurta) based on COI gene markers in Sunda Strait, Cilacap, and Banyuwangi in supporting fisheries management.
Abstrak: Ikan kembung lelaki (Rastrelliger kanagurta) memiliki nilai ekonomis penting bagi nelayan. Ikan pelagis kecil di WPPNRI 572 dan WPPNRI 573 telah mengalami kondisi fully-exploited. Tekanan eksploitasi yang tinggi akan menurunkan keragaman genetik spesies. Penelitian ini bertujuan untuk memastikan spesies, menganalisis kekerabatan dan keragaman genetik secara molekuler ikan kembung lelaki dengan menggunakan marka gen COI di Perairan Selat Sunda, Cilacap dan Banyuwangi. Isolasi dan ekstraksi DNA menghasilkan 30 DNA total, lalu diamplifikasi dengan metode PCR. Hasil sekuensing divalidasi dengan fitur BLASTn pada aplikasi MEGA 11, menunjukkan bahwa 30 contoh dari ke tiga lokasi merupakan spesies R. kanagurta dengan kemiripan antara 98,15% hingga 100%. Jarak genetik ikan kembung lelaki di tiga lokasi memiliki nilai yang rendah. Hasil pohon filogeni menunjukkan contoh memiliki hubungan kekerabatan yang dekat dan adanya pencampuran populasi dari tiga lokasi, sehingga dapat dikelola dalam satu unit pengelolaan., The Indian Mackerel has important economic value for fishermen. The small pelagic fisheries in WPPNRI 572 and WPPNRI 573 have experienced a fully-exploited condition. High exploitation pressure will reduce the genetic diversity of Indian Mackerel species in the wild. This study aims to determine the species, analyze the molecular kinship and genetic diversity of Indian Mackerel (Rastrelliger kanagurta) using COI gene markers in Sunda Strait waters, Cilacap and Banyuwangi. DNA isolation and extraction yielded 30 total DNA, amplified by the PCR method. Sequencing results were validated with the BLASTn feature on the MEGA 11 application, showing that 30 samples were R. kanagurta species with the highest similarity of 100%. The genetic distance of Indian Mackerel in three locations has a low value. The results of the phylogeny tree show that the sample has a close relationship, and there is a mixing of populations from three locations so that it can be managed in one management unit.
Keyword: COI, genetic, Rastrelliger kanagurta

Judul: Molecular identification of the indian mackerel (Rastrelliger  kanagurta) based on 16S rRNA gene markers in FMA NRI 572 and 573 as a basis  for fisheries management
Abstrak: Ikan kembung lelaki (Rastrelliger kanagurta) memiliki nilai ekonomis penting bagi nelayan. Ikan pelagis kecil di WPP-NRI 572 dan WPP-NRI 573 telah mengalami kondisi fully-exploited. Penelitian ini bertujuan memastikan spesies, keragaman genetik dan kekerabatan spesies secara molekuler ikan kembung lelaki (Rastrelliger kanagurta) berdasarkan marka gen 16S rRNA di WPP-NRI 572 dan 573 sebagai dasar pengelolaan perikanan. Penelitian dilakukan pada bulan Juni 2021 hingga Januari 2022. Sampel ikan diambil dari perairan Labuan, Cilacap, dan Banyuwangi dengan jumlah total sebanyak 9 individu. Urutan basa nukleotida hasil sekuensing dianalisis menggunakan software MEGA X. Hasil identifikasi DNA barcoding, menunjukkan spesies ikan kembung dari tiga lokasi berbeda merupakan ikan kembung lelaki (Rastrelliger kanagurta). Nilai keragaman genetik ikan kembung lelaki dari tiga lokasi termasuk dalam kategori tinggi. Hal ini menunjukkan populasi ikan kembung tergolong stok yang baik, The Indian Mackerel has important economic value for fishermen. The small pelagic fisheries in WPP-NRI 572 and WPP-NRI 573 have experienced a fully-exploited condition. This study aims to determine species certainty, genetic diversity and genetic relationships of indian mackerel (Rastrelliger kanagurta) molecularly based on 16S rRNA gene markers in WPP-NRI 572 and 573 as a basis for fisheries management. The research was conducted from June 2021 to January 2022. Fish samples taken from the waters of Labuan, Cilacap, and Banyuwangi with a total of 9 individuals. The nucleotide base sequences of the sequencing results were analyzed using MEGA X software. The identification results of DNA barcoding showed that the mackerel species studied from three different locations were the Indian Mackerel (Rastrelliger kanagurta). The results showed that the genetic diversity value of male mackerel from three locations was included in the high category indicating that the mackerel population was classified as a good stock.
Keyword: 16S rRNA genes, genetic diversity, Rastrelliger kanagurta

Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4