Judul: Pengaruh status ekonomi keluarga terhadap motif menikah dini di Perdesaan
Abstrak: Pernikahan yang dilakukan pada perempuan di bawah umur berkaitan dengan kesiapan fisik maupun psikis yang belum mencapai kematangan termasuk pembentukan identitas diri maupun sosial individu sebagai remaja yang berada pada masa pencarian identitas. Tujuan penelitian ini adalah untuk mengidentifikasi motif-motif dan faktor yang melatarbelakangi pernikahan dini yang terjadi, serta menganalisis hubungannya terhadap pembentukan identitas. Penelitian ini dilakukan dengan menggunakan metode penelitian survei. Pengumpulan data dilakukan menggunakan teknik purposive sampling dengan jumlah responden sebanyak 30 orang. Pengujian pengaruh antara variabel faktor-faktor menikah dini terhadap motif menikah dini dilakukan dengan menggunakan uji regresi berganda, sedangkan variabel pembentukan identitas diuji melalui pendekatan kualitatif deskriptif. Hasil yang diperoleh menunjukkan bahwa pernikahan dini yang terjadi dilatarbelakangi oleh motif remaja untuk memenuhi kebutuhan akan keamanan, sosial, dan harga diri. Pembentukan identitas yang terbentuk pada remaja putri yang melakukan menikah dini ialah pembentukan identitas diri kuat dan pembentukan identitas sosial lemah., A marriage of under age women related with physical as well as psychological state of readiness which have not reached maturity include the formation of self identity and social identity as an adolescence that in fact are on the search for identity. The purpose of this research is to identify the motives and factors which aspects influenced early marriages that occurred, as well as analyzing its relationship towards the formation of identity. The research was carried out using survey research methods. Data collection was done using a purposive sampling technique with 30 respondents. Influence testing between variables factors of early married toward motives of early married conducted using test of multiple regression while formation of identity tested through by qualitatif description. The result showed that early marriage occurred by adolescence motives to fulfill the security, social, and self esteem. The identity formation related in adolescence who married early is strong formation self identity and weak formation social identity.
Keyword: Pernikahan dini, Faktor-faktor menikah dini, Motif menikah dini, Pembentukan identitas

Judul: The Correlation Between Perception of Merarik Culture and The Socio Economic Status with The Motivation to Engage in Early Marriage
Abstrak: Pernikahan dini memberikan beragam dampak negatif bagi individu yang melaksanakannya, oleh karena itu perlu diketahui motivasi mendorong munculnya fenomena tersebut. Berdasarkan sumbernya motivasi dibagi menjadi dua yaitu motivasi internal berupa persepsi individu terhadap budaya merarik, dan eksternal berupa status sosial ekonomi. Penelitian ini bertujuan untuk menganalisis hubungan antara persepsi terhadap budaya merarik dan status sosial ekonomi dengan motivasi melakukan pernikahan dini di Desa Aikmel. Pendekatan yang digunakan dalam penelitian ini adalah pendekatan kuantitatif yang didukung oleh data kualitatif. Data akan dianalisis menggunakan analisis korelasi rank spearman. Instrumen utama yang digunakan dalam penelitian ini adalah kuesioner, dan wawancara mendalam digunakan untuk mendapatkan data pendukung kualitatif. Responden dalam penelitian ini adalah masyarakat Desa Aikmel yang melakukan pernikahan dini dengan budaya merarik berjumlah 67 orang yang di pilih secara purposive. Hasil penelitian menunjukan terdapat hubungan yang kuat dan signifikan antara persepsi terhadap budaya merarik dan motivasi melakukan pernikahan dini, sedangkan status sosial ekonomi dan motivasi melakukan pernikahan dini tidak memiliki hubungan yang signifikan., Early marriage has various negative impact. Therefore its necessary to know the motivation that drives that phenomenon. Based on its resources motivations divided by two. Internal in the form of individual perception and external in the form of socio economic status This research aims to analyze the relationship between perceptions of Merik culture and family socio-economic status with motivation for early marriage in Aikmel Village. The approach used in this research is a quantitative approach supported by qualitative data. Data will be analyzed using Spearman rank correlation analysis. The main instrument used in this research was a questionnaire, and in-depth interviews were used to obtain qualitative supporting data. The respondents in this research were the people of Aikmel Village who entered into early marriages with the Merarik culture, totaling 67 people who were selected purposively. The results of the research show that there is a strong and significant relationship between the perception of Merik culture and motivation for early marriage, while socio-economic status and motivation for early marriage do not have a significant relationship.
Keyword: merarik, motivasi, pernikahan dini, persepsi, motivation, Perception, early marriage

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.