Judul: Clustering Menggunakan Self Organizing Maps (Studi Kasus: Data Perkembangan Anak di Kabupaten Bogor)
Abstrak: Child development data which were gathered by the team from the Department of Family and Consumer Science, Bogor Agricultural University, require data processing to assess the characteristics of child development in Kabupaten Bogor. The purpose of this research is to implement the Self Organizing Maps (SOM) algorithm for data clustering and to obtain the characteristics from the clustering results. The data were obtained from 71 childrens at 2.5–3.4 years of age, 97 childrens at 3.5–4.4 years of age, and 126 childrens at 4.5–5.4 years of age. The data consist of four attributes: Cognitive, Language, Gross Motor, and Fine Motor. These data were the input for SOM algorithm. SOM clustering result was validated using Davies-Bouldin Index. The research shows that the clustering result for children at 2.5–3.4 years of age is 3 clusters, 3.5–4.4 years of age is 4 clusters, and 4.5–5.4 years of age is 3 clusters.
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Judul: Clustering menggunakan self organizing maps studi kasus:data PPMB IPB
Abstrak: Perkembangan teknologi mengakibatkan meningkatnya data dalam jumlah besar. Data berukuran besar yang sudah disimpan tidak digunakan secara optimal karena manusia seringkali tidak memiliki waktu dan ilmu yang cukup untuk mengelolanya. Kasus ini terjadi di Panitia Penerimaan Mahasiswa Baru Institut Pertanian Bogor (PPMB IPB). Penelitian ini bertujuan untuk mengimplementasikan Algoritma Self Organizing Maps (SOM) dalam clustering data, dan untuk mendapatkan karakteristik data dari hasil clustering.
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Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable