Judul: Alel Monomorfik Gen Osteopontin (OPN|BsrI) pada Sapi Bali dengan Metode PCR-RFLP
Abstrak: Bangsa sapi bali sebagai sumberdaya genetik ternak asli Indonesia memiliki keunggulan, salah satunya adalah daya reproduksi yang tinggi. Gen osteopontin (OPN) merupakan anggota dari alur POU class 1 homeobox 1 (POU1F1) yang berkaitan dengan sifat reproduksi. Penelitian ini bertujuan untuk mengidentifikasi ada tidaknya polimorfisme gen osteopontin (OPN) sebagai pengontrol sifat reproduksi pada sapi bali di Pulau Bali dengan menggunakan metode PCR-RFLP. Sampel sapi bali sebanyak 100 sampel yang berasal dari BPTU sapi bali dideteksi keberadaan keragaman genetiknya pada gen OPN menggunakan enzim restriksi BsrI yang memotong sekuens basa ACTGG|N. Amplifikasi gen OPN menghasilkan fragmen dengan panjang 290 bp yang terletak pada intron 4 dengan suhu annealing 60 oC selama 20 detik. Hasil analisis PCR-RFLP menunjukkan sapi bali yang dianalisis memiliki alel monomorfik T adalah 100%. Hasil sekuens fragmen gen OPN intron 4 menunjukkan terdapat mutasi antara basa sitosin (C) dengan timin (T).
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Judul: Identifikasi Keragaman Fragmen Promotor Gen Myostatin (MSTN|MspI) pada Sapi Bali menggunakan Metode PCR-RFLP.
Abstrak: Sapi bali memiliki kemampuan produksi, reproduksi, dan persentase karkas yang tinggi serta mampu beradaptasi pada kondisi lingkungan di Indonesia. Perbaikan mutu genetik sangat diperlukan untuk meningkatkan potensi yang dimiliki sapi bali. Gen myostatin merupakan gen yang memiliki peranan dalam mengatur pertumbuhan otot dan kualitas daging sebagai regulator negatif pertumbuhan. Penelitian ini bertujuan mengidentifikasi keragaman gen myostatin dengan metode Polymerase Chain Reaction – Restriction Fragment Length Polymorphism (PCR-RFLP). Sekuen primer gen myostatin forward dan reverse yang digunakan pada penelitian ini daerah promotor. Fragmen promotor dipotong dengan enzim MspI. Sampel DNA sapi bali yang digunakan berjumlah 44 sampel yang berasal dari BPPTU-HPT Denpasar, Bali. Hasil penelitian didapatkan bahwa gen myostatin daerah promotor pada sapi bali bersifat monomorfik dengan genotipe CC dan frekuensi alel C adalah 1 dan alel T adalah 0.
Keyword: enzim MspI, gen myostatin, PCR-RFLP, sapi bali

Judul: Thermal Characteristics and Dynamics of Tropical Cyclone over Western North Pacific Ocean: A Case Study of Typhoon Songda (Chedeng) 2011
Abstrak: The frequent occasion of Tropical Cyclone (TC) over Western North Pacific (WNP) Ocean lies from 10˚N to 26˚N and 121˚E to 170˚E. Sources of TC development come from warm ocean temperature where latent heat is released during condensation processes and subsequently concentrated in the boundary layer. Relative humidity at 850-700 hPa is responsible to produce the most intense storm. Meanwhile, weak vertical wind shear between 850-200 hPa is one of the dynamical factors that lead to the development of TC. Pressure level of 850-200 hPa is chosen to minimize the thermodynamic effect in the lower troposphere. Typhoon Songda (Chedeng in the Philippines) or TSC lasted from May 18th to 30th, 2011 and strengthened into powerful Super Typhoon stage between May 24th and 27th. Long curving track and the exhibition of all sequences of TC life cycle, TSC is then considered as an ideal case of TC. The objective of the study with case of TSC is to investigate the role of thermal characteristics and dynamics on TC intensity. The research is carried out by using Advanced Research of Weather Research and Forecasting model (WRF ARW model) version 3.3. Two domains are employed in the model. The first domain situate from 02˚N to 40˚N and 118˚E to 149˚E and cover spatial resolution () of 33.1 km. The second domain situate from 09˚N to 27˚N and 119˚E to 132˚E with of 11 km. Timestep of the model () is 120 seconds. The model is supported by the following physical parameterization, i.e., Yonsei University Scheme (YSU) in the boundary layer, Kain-Fritsch scheme (KF) in the cumulus, and WRF Single-Moment 3-class scheme in the microphysics. Initial meteorological condition prior to the formation of the cyclone is generated using WRF ARW standard-release model. Sensitivity of the model output is obtained from a selected single-parameter change scenarios, namely, 10 % to 20 % decrease and increase of wind shear initial data at 850-200 hPa, and by 10 % to 20 % decrease of relative humidity initial data at 850-700 hPa. The result showed that the negative correlations between wind shear and intensity are found in wind shear scenarios. Wind shears of 10 ms-1 which lead to strengthening of the wind intensity in the early period of Typhoon development are found in wind shear scenarios. The effect of wind shear changes on wind intensity is statistically significant in the 20 % wind shear scenarios. During mature stage, weak wind intensity occurred when wind shear increase as given in the WRF standard-release and the 20 % decreasing scenario. Different situation is found in the 20 % increasing scenario where wind shear tend to weak and lead to strong wind intensity at this stage. Meanwhile, the reduction of moisture supply at 850-700 hPa resulting from 20 % decreasing relative humidity play a major role in the weakening of wind intensity and reduces mechanical energy of the cycle process as much as 300 J kg-1.
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