Judul: Konstruksi Gen Kitinase untuk Ketahanan terhadap Ganoderma pada Vektor Ekspresi pCambia 1303
Abstrak: Efforts to increase palm oil production face problems associated with pest and diseases caused by pathogenic fungus such as Ganoderma spp. A new approach to obtain palm oil transgenic is by inserting a gene encoding antifungi compound. One of genes is chitinase gene derived from Trichoderma. The research targets are to insert chitinase gene into expression vector pCambia 1303 between the 35S Cauliflower Mosaic Virus (CaMV) promoter and the root specific promoter. The Chitinase gene was cloned into pCAMBIA 1303 expression vector followed by transformation of recombinant plasmid into Escherichia coli XL-1 Blue and transferred into Agrobacterium tumefaciens. Results showed that the chitinase gene was successfully inserted into pCambia 1303. Restriction of the recombinant DNA using restriction enzymes Nco1 and Spe1 resulted in two bands of 1200 bp which is pCambia 1303 and of 1500 bp which is the chi gene. The recombinant DNA was successfully transformed into Agrobacterium tumefaciens using competent cell of AGLO strain. The success of transformation was analyzed by restricting recombinant DNA isolated from transformant colonies using NcoI and SpeI restriction enzymes.
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Judul: Construction of Gene Chitinase in Expression Vector by Method Gateway and Transformation to Agrobacterium sp.
Abstrak: Kelapa sawit merupakan tumbuhan industri penting penghasil minyak goreng, minyak industri, maupun bahan bakar. Usaha peningkatan produksi perkebunan kelapa sawit tersebut secara terus menerus dilakukan antara lain untuk mengatasi masalah hama dan penyakit yang disebabkan oleh Ganoderma spp yang dikenal dengan nama penyakit busuk akar. Salah satu cara untuk menanggulangi masalah ini adalah dengan menyisipkan gen penghasil zat yang berfungsi sebagai antijamur salah satunya yaitu gen penyandi kitinase. Penelitian ini bertujuan untuk membuat konstruksi gen penyandi kitinase dalam vektor ekspresi dengan metode Gateway yang cepat dan terarah. Gen penyandi kitinase yang berukuran 1500 pb dari penelitian sebelumnya oleh Novianthy (2009) terlebih dahulu disisipkan ke dalam vektor donor selanjutnya ke dalam vektor ekspresi. Pengujian gen penyandi kitinase yang telah tersisipkan ke dalam vektor ekspresi menghasilkan pita DNA berukuran 1500 pb yang ditunjukkan oleh elektroforegram PCR koloni gen penyandi kitinase. Vektor ekspresi yang telah tersisipi gen penyandi kitinase selanjutnya ditransformasikan ke dalam Agrobacterium tumefaciens galur AGL-0. Pengujian PCR koloni menunjukkan gen penyandi kitinase telah berhasil disisipkan pada A. tumefaciens. Gen penyandi kitinase siap ditransfer ke dalam tanaman.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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