Judul: Prevalensi anemia dan perilaku makan remaja puteri di SMUN 2 Kuningan kabupaten Kuningan
Abstrak: Penelitian ini secara umum bertujuan untuk mengetahui prevalensi anemia dan perilaku makan remaja puteri SMUN 2 Kuningan. Adapun tujuan khususnya adalah untuk mengetahui prevalensi anemia remaja puteri, karakteristik contoh dan keluarganya, pengetahuan tentang anemia, sikap tentang anemia, kebiasaan makan, dan tingkat konsumsi protein, zat besi, vitamin A dan vitamin C, serta mengetahui perbedaan pengetahuan, sikap, kebiasaan makan, dan tingkat konsumsi antara remaja puteri anemia dengan non anemia. Penelitian ini dilakukan di SMUN 2 Kuningan yang berada di Kabupaten Kuningan, Jawa Barat, dari Bulan April sampai Mei 2001. Contoh dalam penelitian ini adalah remaja puteri Kelas 1 dan Kelas 2. Jumlah contoh diambil secara proporsional adalah 20% dari jumlah siswa yang diperiksa (236) yakni 48 orang yang diambil secara Systematic Sampling. Data yang dikumpulkan adalah data primer dan data sekunder. Data primer meliputi kadar Hb untuk menentukan status anemia dengan menggunakan metode Sahli, keadaan umum contoh, keadaan sosial ekonomi keluarga, pengetahuan, sikap, kebiasaan makan yang dikumpulkan melalui kuesioner, serta konsumsi pangan dengan metode recall. Data sekunder meliputi keadaan umum daerah dan sekolah. Analisis data dilakukan secara statistik deskriptif dan inferensial yang menggunakan uji Man Whitney U dan t-test dengan menggunakan program komputer Excel 2000 dan SPSS 10,0 for windows.
Keyword: anemia

Judul: Keragaan Perilaku Remaja Putri terkait Anemia Gizi, Aktivitas Fisik, dan Kebiasaan Makan di Pondok Pesantren Ummul Quro Bogor.
Abstrak: Proporsi anemiapada remaja putri usia 10-18 tahun di Indonesia berdasarkan SKRT tahun 2012 sebesar 57.1%. Pengetahuan tentang anemia dapat mempengaruhi sikap dan perilaku remaja dalam pemilihan makanan yang selanjutnya akan mempengaruhi keadaan gizi termasuk status anemia. Tujuan penelitian ini adalah untuk menganalisis hubungan antara perilaku remaja putri terkait anemia gizi, aktivitas fisik, dan kebiasaan makan pada santri putri di Pondok Pesantren Ummul Quro Bogor. Desain penelitian yang digunakan adalah cross-sectional study dengan jumlah contoh sebesar 78 orang yang merupakan kelas XI IPA. Pengetahuan, sikap, dan praktik diperoleh melalui pengisian kuesioner, aktivitas fisik diperoleh melalui recall aktivitas 2x24 jam, konsumsi pangan diperoleh melalui food recall 2x24 jam, serta kebiasaan makan diperoleh melalui food frequency questionnaire (FFQ). Hasil penelitian menunjukkan bahwa pengetahuan dan sikap tentang anemia pada sebagian besar contoh tergolong cukup, sedangkan praktik anemia tergolong kurang. Terdapat hubungan yang signifikan pada aspek sebab-akibat anemia antara domain pengetahuan dan sikap(r= 0.265, p= 0.019). Tidak terdapat hubungan yang signifikan antara perilaku anemia dengan asupan dan tingkat kecukupan gizi pada contoh.
Keyword: aktivitas fisik, anemia, pengetahuan, praktik, sikap

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable