Judul: Isolasi Bakteri Proteolitik, Karakterisasi, dan Aplikasi Enzim Protease pada Kokon Cricula trifenestrata
Abstrak: Goldsilk is silk fiber that made by human which it get from Cricula trifenestrata cocoon. Existence of proteolitic bacterias from cocoon have potential to influence quality of goldsilk. The aims of the experiment were to get proteolytic bacteria, characterize the protease enzyme and apply protease on goldsilk cocoon. A total of 26 proteolytic bacteria were isolated from good and bad cocoons. Nutrient Broth (NB) and Nutrient Agar (NA) containing 1% skim milk used as growth media. Isolate CKH 7 and CKK 7 are selected for protease production because they have the highest protease activity. CKH 7 and CKK 7 were identified as Aeromonas salmonicida ssp salmonicida and Vibrio fluvialis. Highest protease productivity reached after 9 hour incubation. Optimum protease activity of isolate CKH 7 at pH 8 and temperature 25 °C. Its activity increased by the presence of 2 mM Na2+ and Ca2+. Activity isolate CKK 7 optimum showed at pH 5, reached after 12 hour incubation and temperature 35°C. Its activity increased by the presence of 2 mM Cu2+, Co2+ and Ca2+. Protease of CKH 7 characterized as metalloprotease. Result of submerge cocoon in protease and continued by NaOH 0,1 N are fiber be clean, shiny, and not easy cut off.
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Judul: Isolasi dan Identifikasi Bakteri Kitinolitik Penghambat Pertumbuhan Cendawan Patogen Asal Kokon Cricula trifenestrata
Abstrak: Gold Silkworm’s cocoon is a coat of Cricula trifenestrata’s pupa that include part of life cycle. The fiber which is resulted from processing cocoon has high economic value because the specific of gold colour that doesn’t have any else cocoon. However, on the process often occurred many problems that could decrease quantity and quality of cocoon, such as attack from pathogenic fungi. For controlling of the pathogenic fungi has been used chemical agent, but using regularly caused some negative effects. As alternative using chitinolytic bacteria as biocontrol, because of chitin is the highest component of the fungi cell wall. Therefore the objectives of this research were to isolate and identify of chitinolytic bacteria that inhibited fungi pathogen which isolated from cocoon of C. trifenestrata. 17 strains chitinolytic bacteria were isolated from two samples cocoon of health pupa and sick pupa. There were four isolates CH2, CH10, CS1, and CS4 had highest value of chitinolytic index. CH10 and CS1 strains showed highest chitinase activity, both isolates were identified using MicrogenTM GenA+B ID System and categorized as Shewanella putrefaciens. Scopulariopsis sp. and FC (unidentified) were isolated from cocoon of sick pupa. Strain CH10 inhibited FC on culture bacteria test but not from its in chitinase. Scopulariosis sp. had been inhibited by culture and its chitinase.
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Judul: Perbandingan Berbagai Metode Estimasi Curah Hujan Menggunakan Data Himawari-8 di Malang, Jawa Timur
Abstrak: Estimasi curah hujan dengan menggunakan penginderaan jauh dapat dimanfaatkan untuk mengatasi masalah pengamatan curah hujan. Saat ini, terdapat beberapa metode untuk memperkirakan curah hujan dengan menggunakan data satelit sehingga dapat dibandingkan antar metode. Penelitian ini menggunakan data Himawari-8 untuk membandingkan estimasi nilai curah hujan menggunakan tiga metode, yaitu i) Auto Estimator (AE), ii) Convective Stratiform Technique (CST), dan iii) Modified Convective Stratiform Technique (CSTm). Ketiga metode tersebut dievaluasi dengan menggunakan data curah hujan harian pada bulan Januari dan Februari 2016 yang diambil dari sembilan stasiun di Malang, Jawa Timur. Secara umum, metode CST menghasilkan estimasi yang lebih baik dibandingkan dengan metode AE dan CSTm. Estimasi curah hujan untuk Januari 2016 dari ketiga metode cenderung lebih tinggi dibandingkan dengan data observasi curah hujan yang diamati. Sebaliknya, perkiraan curah hujan pada Februari 2016 cenderung lebih kecil dibandingkan dengan data observasi. Nilai korelasi positif tertinggi pada bulan Januari diperoleh menggunakan metode CST di Stasiun Dampit (r = 0.79), sedangkan pada bulan Februari kemudia juga menggunakan metode CST di Stasiun Kalipare (r = 0.75). Nilai maksimum RMSE yang diperoleh pada bulan Januari adalah 51.5 untuk metode CST Stasiun Dampit, yang lebih baik dari kisaran RSME yang diperoleh dari dua metode lainnya.
Keyword: data satelit, estimasi curah hujan, Himawari-8, metode evaluasi