Judul: Modeling Land-Use Change and Its Driving Factors (A preliminary dynamic landscape-based model of Sumberjaya Watershed
Abstrak: The prototype land-use model in this research is intended to provide support for negotiation making processes with information on the dynamics of land use change under some combinations of economic scenarios. The main characteristic of the model is to integrate economic and spatial dynamics. A combination of system dynamics and constrained cellular Automata simulates changes in land use driven by economic factors. At its current status, it provides insight of past and simulated land use changes based on economic scenarios.
Keyword: 

Judul: Forecasting Land Use Change and the Impact on Water Yield at Watershed Scale
Abstrak: One of the most important issues in watershed management is the use of land, especially in the upstream catchment area. People effort to fulfill their ends meet can cause land conversion. As a result of the land conversion, the Cisadane River condition seems quite apprehensive in the last two decades. Environmental degradation in Cisadane watershed in both the upstream and downstream has a heavy impact to the availability of water resources in Cisadane River. In relation to this fact, an in-depth study on land use change in Upstream Cisadane Watersheds and its dynamics need to be conducted. Particularly, the impact of land use change on water yield. This research purpose is to forecast the future of land use changes (using CLUE-S) and assess the impact of the predicted land use changes on water yield in Upstream Cisadane Watershed (using HEC-HMS). This research was conducted in Upstream Cisadane Watershed – Bogor. The study area is Upstream Cisadane Watershed with outlet of watershed in Empang, which located in both Bogor Regency and Bogor Municipality. Geographically, it is located between 6036’26.05” – 6047’08.49” South Latitude and 106044’30.27” – 106056’36.76” east longitude. Four scenarios are developed based on land use demand (population growth) and area restriction (spatial policy). The scenarios presented are not necessary the most realistic, but are made in such a way that they provide information on the functioning of the model. Binomial logistic regression is used to examine the relation between land use and possible driving factor. The result of this analysis are coefficient values that shows the contribution of each driving factor to land use change. ROC (Relative Operating Characteristics) is a method to measure the goodness of the statistical model. The probability of each land use resulted from logistic regression is compared to the real land use map to calculate the equal category of each grid cell between those maps. This method will depict the capability of regression equation to represent land use characteristics. The goodness of the statistical measurement revealed that ROC values for urban water area, grassland area, estate area, settlement area and forest area were 0.903, 0.701, 0.780, 0.813 and 0.994, which indicated that the probability of land uses built from these models were capable to represent land use changes and empirical analysis by using logistic regression method was satisfactory to examine the relationship between driving factors and land use change in study area. Through model validation process shows that overall accuracy and Kappa accuracy for land use simulation are 90.83 % and 86.00 % or categorized as fit. It indicates that the driving factors have a good capability to explain land use pattern in study area and it can be used to predict future land use pattern. According to the change detection for the land use classification year 1991, simulated year 2000, year 2010, year 2020 and year 2030, it can be seen that settlement, forest and grassland area are increasing over time, whereas estate tend to decrease and water remains constant. Based on the findings, settlement area were significantly increase during the period 1991-2009 and causing
Keyword: Bogor Agricultural University (IPB)

Judul: Influenza Specific Immunoglobulin Y Purification from Egg Yolk with Sodium Chloride Precipitation Method
Abstrak: Imunoglobulin Yolk (IgY) yang terdapat dalam kuning telur telah banyak dimanfaatkan untuk imunoterapi dan imunodiagnostik. Salah satu kendala dalam pemurnian IgY dari kuning telur adalah pemisahan Ig dengan lemak, sehingga teknik pemurnian Ig selalu berkembang. Penelitian ini bertujuan untuk melakukan pemurnian IgY dari kuning telur dengan metode pengendapan menggunakan sodium chloride (NaCl) serta melakukan karakterisasi IgY spesifik influenza. Sodium chloride dipilih karena bahan kimia ini relatif murah, ramah lingkungan, dan tidak merusak imunoaktivitas IgY. Kuning telur yang digunakan dalam penelitian ini berasal dari telur ayam layer yang divaksinasi dengan vaksin influenza Flubio® yang berisi virus Influenza A/H3N2, A/H1N1 dan B/Washington. Pemurnian IgY hanya dilakukan pada kuning telur yang mengandung antibodi spesifik influenza. Deteksi keberadaan antibodi spesifik influenza ditentukan dengan uji agar gel presipitasi (AGP) dan metode enzyme linked immunosorbent assay (ELISA). Konsentrasi IgY hasil pemurnian dihitung dengan spektrofotometer dan karakterisasi protein dilakukan dengan metode Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Konsentrasi protein dari hasil pemurnian IgY memiliki nilai rata – rata 50,403 mg/mL. Hasil SDS-PAGE menunjukkan enam pita protein, yaitu struktur IgY utuh (174,63 kDa), Apoprotein-LDL (129,77 kDa), rantai berat IgY (66,54 kDa), Fab ( 47,65 kDa), rantai ringan IgY (21,85 kDa), dan β-livetin (36,74 kDa). IgY hasil pemurnian masih dapat berikatan dengan antigen influenza berdasarkan uji ELISA. Hasil penelitian ini menunjukkan bahwa pemurnian IgY menggunakan metode pengendapan NaCl dapat memurnikan IgY dengan struktur utuh, meskipun masih ada dua protein tidak spesifik hasil pemurnian. IgY yang dapat berikatan dengan antigen influenza mengindikasikan prospek pemanfaatannya untuk pencegahan influenza dan imunodiagnostik., Yolk immunoglobulin (IgY) contained in egg yolk has been widely used for immunotherapy and immunodiagnostics. One of the obstacles in IgY purification from egg yolk is separating Ig from fat, so that IgY purification techniques are always developing. This study aimed to purify IgY from egg yolk using sodium chloride (NaCl) precipitation method and to characterize influenza-specific IgY. Sodium chloride was chosen because this chemical is relatively unexpensive, environmentally friendly, and does not impair IgY immunoactivity. The egg yolks used in this study were collected from layer eggs vaccinated with the Flubio® influenza vaccine, which contains the Influenza A/H3N2, A/H1N1 and B/Washington viruses. IgY purification was only carried out on egg yolks containing influenza specific antibodies. The presence of influenza-specific antibodies was determined by the agar gel precipitation (AGP) test and the enzyme linked immunosorbent assay (ELISA) method. The purified IgY concentration was determined using spectrophotometer, and protein characterization was performed using the Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) method. Protein concentration from purification has a mean value of 50,403 mg/mL . SDS-PAGE result showed six protein bands, namely intact IgY structure (174,63 kDa), Apoprotein-LDL (129,77 kDa), IgY heavy chain (66,54 kDa), Fab (47,65 kDa), IgY light chain (21,85 kDa), and β-livetin (36,74 kDa). The purified IgY still able to bind with influenza antigen based on the ELISA test. The results of this study indicate that purification of IgY using the NaCl precipitation method can purify IgY with intact structure, although there are still two unspecific proteins in the purification results. The resulting IgY can bind to influenza antigens indicating the
Keyword: Immunoglobulin Y, influenza, egg yolk, purification, sodium chloride