Judul: Hubungan Berat Badan LAhir Bayi Dismature dengan Morbiditas dan Status Gizi pada Bayi Umur 1-6 Bulan
Abstrak: Tujuan penelitian ini untuk mengetahui : berat badan lahir bayi dismature; pertumbuhan fisik; morbiditas bayi; status gizi bayi; pengetahuan gizi ibu; konsumsi bayi; mengetahui hubungan berat badan lahir bayi dismature dengan morbiditas; berat badan lahir bayi dismature dengan status gizi bayi; morbiditas dengan status gizi bayi; berat badan lahir bayi dismature dengan pertumbuhan fisik, pengetahuan gizi ibu dengan konsumsi bayi.
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Judul: Hubungan antara Kebiasaan Makan, Antenatal Care, dan Status Gizi Ibu Hamil dengan Berat dan Panjang Badan Bayi Lahir
Abstrak: Masa kehamilan merupakan tahap awal dari 1000 HPK, dimana masa ini dapat mempengaruhi status kesehatan di periode selanjutnya. Keberhasilan kehamilan dapat diketahui dengan indikator berat dan panjang lahir. Tujuan penelitian adalah menganalisis hubungan antara kebiasaan makan, pemeriksaan kesehatan, dan status gizi ibu hamil dengan berat dan panjang badan bayi lahir di Desa Gobang, Kecamatan Rumpin, Kabupaten Bogor. Desain penelitian adalah cross-sectional study dengan jumlah subjek 32 orang ibu yang memiliki bayi berusia maksimal 3 bulan. Hasil penelitian menunjukkan bahwa terdapat 9.4% subjek yang memiliki bayi BBLR (<2500 g) dan 21.9% subjek yang memiliki bayi dengan panjang lahir rendah (<48 cm). Terdapat hubungan yang signifikan antara berat lahir dengan LLA, kunjungan ANC pertama kali, konsumsi TTD, kebiasaan makan makanan pokok, buah, cemilan, dan susu (p<0.05), serta frekuensi ANC, konsumsi TTD, dan kebiasaan makan protein hewani dengan panjang lahir (p<0.05).
Keyword: ANC, BBLR, ibu hamil, riwayat kehamilan, stunting

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.