Judul: Analisis keragaman genetik labi-labi (Amyda cartilaginea) berdasarkan genom mitokondria
Abstrak: Amyda cartilaginea belongs to the Order Testudinata, Suborder Cryptodira and Family Trionychidae. Recently, the population of A. cartilaginea has been decreasing because of human exploitation and environmental destruction. Therefore, it is important to carry on the research in genetic diversity among this spesies. The research was conducted using the mitochondrial DNA marker by Polymerase Chain Reaction-Restriction Fragment Length Polymorphisms (PCR–RFLP) technique. PCR technique was conducted to observe genetic diversity of mtDNA using a pair of primer 577 and 578. The amplified region was control region which flanked tRNA Pro and tRNA Phe based on reference sequence of Dogania subplana. The amplification result ed in the product of 500 base pairs and 710 base pairs. The PCR product was then cut by restriction endonuclease, namely Hha I (GCG?C), HaeIII(GG?CC), AluI (AG?CT), MboI (?GATC) and DraI (TTT ?AAA). Twelve haplotypes was found among 39 samples.. Haplotype 2 showed wide distribution compared to other haplotypes. Nucleotide diversity among haplotypes is 5.4 %.
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Judul: Genetic Differentiation and Labi-Labi  Distribution Amyda cartilaginea in Populations in Northern Kalimantan
Abstrak: Labi-labi is a freshwater turtle with a soft shell and is widespread in some Asian countries. Indonesia has a species of labi-labi that is spread naturally, one of which is Amyda cartilaginea. The research was conducted using mitochondrial d loop DNA as a genetic marker. The study aims to study the genetic variation and the phylogeographic relationship of A. cartilaginea in the Kalimantan population. Peripheral samples of carapace and liver labi-labi A. cartilaginea are collected in the laboratory as a source of DNA molecules. DNA amplification is performed using the primary pairs AF574 (forward) and AF575 (reverse). The sequencing results had a length of 377 bp and all samples were confirmed as species A. cartilaginea. The genetic variation of A. cartilaginea populations in Northern Kalimantan contains 27 polymorphic sites that form six haplotipes with a haplotype diversity of 0.8444 and a nucleotide variety of 0.02107. The resulting genetic distance ranges from 0.000–0.061 with the phylogeographic tree A. cartilaginea Northern Kalimantan forming a single cluster compared to the distance., Labi-labi merupakan kura-kura air tawar dengan cangkang yang lunak dan tersebar luas di beberapa negara Asia. Indonesia memiliki spesies labi labi yang tersebar secara alami, salah satunya Amyda cartilaginea. Penelitian dilakukan dengan memanfaatkan ruas d-loop DNA mitokondria sebagai penanda genetik. Penelitian ini bertujuan mempelajari variasi genetik serta hubungan filogeografi A. cartilaginea pada populasi Kalimantan. Sampel perifer karapas dan hati labi-labi A. cartilaginea koleksi laboratorium sebagai sumber molekul DNA. Amplifikasi DNA dilakukan menggunakan pasangan primer AF574 (forward) dan AF575 (reverse). Hasil sekuensing memiliki panjang 377 bp dan semua sampel terkonfirmasi sebagai spesies A. cartilaginea. Variasi genetik A. cartilaginea populasi Kalimantan Utara terdapat 27 situs polimorfik yang membentuk enam haplotipe dengan keragaman haplotipe sebesar 0,8444 dan keragaman nukleotida sebesar 0,02107. Jarak genetik yang dihasilkan berkisar 0,000–0,061 dengan pohon filogeografi A. cartilaginea Kalimantan Utara membentuk satu klaster yang dibandingkan dengan jarak.
Keyword: filogeografi, molekuler, mtDNA, PCR, phylogeography, molecular

Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4