Judul: Pertumbuhan Gmelina (Gmelina arborea Roxb) pada Beberapa Pola Agroforestri di Desa Sekarwangi, Kecamatan Malangbong, Kabupaten Garut
Abstrak: Agroforestry is a system of land use which combines wooden plants (trees, shrubs, rattan) with plants is not a wooden or may with grass (pasture), sometimes there is a component of livestock and other animals so that ecological interactions between plant formed a wooden and other components. Selection of inappropriate agroforestry pattern will affect the growth of plants by the existence of high competition to get nutrient elements, air, and light of the Sun. Agroforestry patterns which were selected as a treatment are agroforestry pattern one (AgF1) consists of gmelina, suren, mahoni, pete, the dominance of chili plants, and corn. Agroforestri pattern two (AgF2) consists of gmelina, sengon, dominance of plant corn, and cassava. Agroforestri pattern three (AgF3) consists of gmelina, suren, crop the dominance of cassava, peanut, and corn. Observed parameters is gmelina dimension, which are total tall, total diameter trunk, tall branch without crown, and the crown diameter. This research aims to analyze the effect of agroforestry pattern on the growth of G. arborea and analyze the relationship of land use management system with the growth of G. arborea. The research held in the Sekarwangi Village, Malangbong, Garut in December 2011 up to February 2012. This research uses a Complete random Design (RAL) with one factorial analysis, i.e. agroforestry pattern. The result showed that agroforestry pattern one give the best effect to gmelina growth. It obtained based on the test and test fingerprints variegated Duncan. Agroforestry pattern one gives highest average on all parameters. Besides that, land management system agroforestry pattern one also exert the best effect to the gmelina growth
Keyword: 

Judul: Pertumbuhan tanaman pokok Gmelina arborea Roxb. pada beberapa pola agroforestri di Desa Cikanyere, Kecamatan Sukaresmi, Kabupaten Cianjur
Abstrak: Pemilihan kombinasi tanaman yang dibudidayakan dalam program RHLP Desa Cikanyere memungkinkan terjadinya perbedaan respon bagi pertumbuhan tanaman pokok. Penelitian ini bertujuan untuk mengkaji pertumbuhan tanaman pokok gmelina (Gmelina arborea Roxb.) pada beberapa pola agroforestri serta pengaruh sistem pengelolaan terhadap pertumbuhan tanaman pokok gmelina (Gmelina arborea Roxb.) yang dikembangkan oleh kelompok tani di Desa Cikanyere, Kecamatan Sukaresmi, Kabupaten Cianjur. Survei pendahuluan dilakukan untuk menentukan pola agroforestri yang diteliti. Data dimensi tanaman pokok diambil secara sensus terhadap tanaman gmelina pada masing-masing pola agroforestri yang terpilih berupa tinggi, diameter dan proyeksi tajuk. Parameter tanah yang diamati berupa sifat fisik dan kimia dari contoh tanah masing-masing pola agroforestri yang kemudian dianalisis di laboratorium. Data penutupan tajuk tegakan diambil dengan menggunakan alat spiracle densiometer. Sejarah pengelolaan lahan ditelusuri dengan melakukan kegiatan wawancara terhadap petani pengelola masing-masing pola agroforestri terpilih. Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan pola agroforestri tanaman pokok gmelina yang dominan dikembangkan meliputi AF 1 (gmelina, jagung, singkong, pisang), AF 2 (gmelina, jagung, kopi, pisang), AF 3 (gmelina, mahoni, singkong, petai), AF 4 (gmelina, mahoni, jagung, cabai) dan AF 5 (gmelina, mahoni, padi gogo, singkong, jagung). Pola agroforestri AF 3 menunjukkan pertumbuhan dimensi tinggi dan diameter paling rendah, sedangkan pola AF 1 dan AF 4 menunjukkan pertumbuhan dimensi yang paling tinggi dibandingkan dengan pola lain. Pola AF 3 mempunyai LCR dan LAI yang kecil sehingga proses fotosintesis berjalan kurang optimal sedangkan pada pola AF 1 dan AF 4 nilai LCR dan LAI paling besar sehingga fotosintesis berjalan optimal dan hasilnya pertumbuhan tanaman pokok lebih baik daripada pola yang lain. Unsur fosfor (P) pada pola agroforestri AF 3 diduga menjadi faktor pembatas bagi pertumbuhan tanaman pokok gmelina. Unsur P dan K dibutuhkan tanaman singkong dalam jumlah yang cukup untuk membentuk akar dan pembesaran umbi sehingga diduga sebagai penyebab rendahnya kandungan unsur P pada pola AF 3. Pemupukan yang paling intensif ditemukan pada pola AF 4 sedangkan yang yang paling rendah pada pola AF 3. Pertumbuhan tanaman pokok gmelina (Gmelina arborea Roxb.) dipengaruhi oleh interaksi antar komponen serta sistem pengelolaan lahan yang dilakukan petani pada masing-masing pola agroforestri.
Keyword: Agroforestry, Gmelina arborea, RHLP

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
Keyword: