Judul: Evaluasi Perseptual Kualitas Estetika dan Ekologi Objek Wisata Kebun Raya Cibodas
Abstrak: The usage of Cibodas Botanical Garden as a recreation area, as well as a collection function with its natural beauty, can change the aesthetics and ecological quality of this garden. An aesthetics and ecological quality assessement with Scenic Beauty Estimation (SBE) and Semantic Differential methode were applied. Eleven area spreading over three zone (service area, collection area, and recreation area) had been assessed their quality and characteristic into three group i.e. good, fair, and bad. Some of them had been changed far enough from their nature. Lawn (recreation area) showed good aesthetics quality and Rhododendron Garden (collection area) have bad aesthetics quality. Result of the evaluation on ecological quality showed that almost all of vantage point in the garden showed good ecological quality except some areas such as Rhododendron Garden and Ciismun Waterfall that have bad quality. Some recommendation how to increase landscape intregrity of the Garden were elaborate on the basis of these research result.
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Judul: Pengelolaan Jalur Wisata Air Terjun Cibeureum sebagai Penyedia Jasa Keindahan Lanskap di Taman Nasional Gunung Gede Pangrango
Abstrak: Landscape beautification service is one of landscape services that provided by Cibeureum Waterfall tourism track. The landscape beautification service is the biggest attraction for visitors. That is why the presence of landscape beautification service should be sustainable. One way to keep the good quality of landscape beautification service is to make a management plan. The making of management plan was started with evaluation of landscape aesthetic quality by using Scenic Beauty Estimation (SBE) analysis and also evaluation of landscape aesthetic characteristics by using Semantic Differential (SD) analysis. Furthermore, it also used SWOT analysis to arrange the recommendation of management plan. The result of SBE analysis showed that the wide and measured images of the landscape were giving a significant impact for landscape aesthetic quality value of Cibeureum Waterfall tourism track. Moreover, any kind of hardscape addition, such as bridge and viewing deck and also water element would give an influence to the quality of landscape aesthetic. From SD analysis, the result showed that modification nor addition of the hardscape type would give an impact to landscape aesthetic characteristics. The value of landscape aesthetic characteristics would be increased if the type of hardscape that was used is in a harmony and fused with the landscape condition. The result of SD analysis then used for factor analysis which showed that the most influence factors for landscape aesthetic characteristics are safety, comfort, and brightness factors. By using the result of SD analysis, it also analyzed with regression analysis which showed that narrow-wide of landscape aesthetic characteristic was giving a significant impact to landscape aesthetic quality. All the results of landscape quality and landscape characteristics evaluation were considered for the management plan recommendation. As the result of SWOT analysis, there are eight recommendations or alternative strategies for Cibeureum Waterfall tourism track management plan.
Keyword: Bogor Agricultural University (IPB), management plan, landscape aesthetic quality, landscape aesthetic character

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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