Judul: Persepsi manfaat dan risiko dalam perilaku pembelian konsumen online shop
Abstrak: The attendance of the internet is used by marketing person to create a new shopping environtment that is e-commerce. One of real forms of the e-commerce is online shop. The purpose of this research was for analizing an example purchasing behaviour. Design of this research was cross sectional study. The election of research location was done with purposively, those are Kaskus, Facebook Online Shop, and Komunitas Online Shop. The pattern election was performed with purposive way that was the consumer who had shopped fashion, book and electronic. The kind of product was most bought by example that does not work that is fashion, meanwhile the example works that is a book. The frequeny of buying of the two example groups are included in low category. The address of online shop was most visited by example that does not work that was Kaskus, meanwhile the example works thas was Facebook online shop. The expense of online shopping by example that does not work including low category, meanwhile for the example that works including very high category. The payment method is had by the example groups was transfer method. Gotten nine factors that become an independent variable those are sex, age, education, job status, income, life style, benefit perception, risk perception, and online searching. Sex, income, benefit perception, and online searching variables are significant factors that influence a purchasing behaviour. Keywords: life style, perceived benefit, perceived risk, searching online, evaluation alternative and purchased behaviour.
Keyword: Persepsi manfaat, Persepsi risiko, Pencarian online, Gaya hidup, Evaluasi informasi, Perilaku pembelian

Judul: The Effect of, Perceived Risk, and Beauty Lifestyle towards Skincare Purchasing Decision on E-commerce
Abstrak: Era digitalisasi mendorong berkembangnya transaksi belanja online (ecommerce). Konsumen sering kali tidak menyadari bahwa dibalik kemudahan berbelanja terdapat risiko yang harus dipertimbangkan. Tujuan penelitian ini adalah menganalisis keputusan konsumen dalam membeli skincare melalui e-commerce. Metode survei yang digunakan untuk pengambilan data adalah teknik nonprobability sampling yaitu voluntary sampling. Data dianalisis dengan analisis deskriptif dan analisis kolerasi, sementara, analisis dilakukan dengan Structural Equation Modeling (SEM) menggunakan SmartPLS. Penelitian ini dilakukan terhadap 196 responden yang merupakan Warga Negara Indonesia (WNI), berusia minimal 17 tahun dan pernah membeli produk perawatan kulit melalui e-commerce. Hasil studi menunjukan bahwa keputusan pembelian responden tergolong baik sedangkan, persepsi risiko dan beauty lifestyle responden tegolong pada kategori sedang. Analisis SEM menunjukkan bahwa beauty lifestyle berpengaruh signifikan terhadap keputusan pembelian konsumen, sedangkan persepsi risiko tidak memiliki efek terhadap keputusan pembelian., The digitalisation era promotes the growth of e-commerce, which enables online shopping transactions. However, consumers may not be aware of the potential risks associated with the convenience of e-commerce. Therefore, this study aims to examine consumer decision making when purchasing skincare products through e-commerce. Non-probability sampling, specifically voluntary sampling, was employed to gather data. Data were analysed using descriptive analysis, and correlation analysis. Additionally, Structural Equation Modelling (SEM) was utilized through SmartPLS for analysis. The study was conducted on 196 Indonesian citizen, aged 17 years old and above, and have purchased skin care products through e-commerce. The study results indicate that the respondents' purchasing decisions were high, whereas their risk perception and beauty lifestyles were classified as medium. The results of the SEM analysis demonstrate that a beauty-oriented lifestyle significantly impacts consumer purchasing decision, whereas risk perception have no significant effect towards purchasing decision.
Keyword: Gaya hidup, keputusan pembelian, persepsi risiko, perawatan kulit

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable