Judul: Analisis keragaman genetik labi-labi (Amyda cartilaginea) berdasarkan genom mitokondria
Abstrak: Amyda cartilaginea belongs to the Order Testudinata, Suborder Cryptodira and Family Trionychidae. Recently, the population of A. cartilaginea has been decreasing because of human exploitation and environmental destruction. Therefore, it is important to carry on the research in genetic diversity among this spesies. The research was conducted using the mitochondrial DNA marker by Polymerase Chain Reaction-Restriction Fragment Length Polymorphisms (PCR–RFLP) technique. PCR technique was conducted to observe genetic diversity of mtDNA using a pair of primer 577 and 578. The amplified region was control region which flanked tRNA Pro and tRNA Phe based on reference sequence of Dogania subplana. The amplification result ed in the product of 500 base pairs and 710 base pairs. The PCR product was then cut by restriction endonuclease, namely Hha I (GCG?C), HaeIII(GG?CC), AluI (AG?CT), MboI (?GATC) and DraI (TTT ?AAA). Twelve haplotypes was found among 39 samples.. Haplotype 2 showed wide distribution compared to other haplotypes. Nucleotide diversity among haplotypes is 5.4 %.
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Judul: Analisis keragaman genetik labi-labi, dogania Subpkma (Testudines: trionychidae), melalui teknik PCR-RFLP
Abstrak: Saat ini telah terjadi penurunan populasi labi-labi yang sebagian besar diakibatkan adanya perburuan liar dan kerusakan lingkungan. Melihat hal itu diperlukan suatu langkah untuk mengumpulkan berbagai informasi biologi labi-labi. Salah satu caranya adalah dengan melakukan anal isis molekular sebelum berbagai informasi lainnya diungkapkan. Penanda molekular genetik yang sering digunakan dalam analisis molekular adalah daerah pengendali (d-loop) genom mitokondria menggunakan teknik PCR-RFLP. Bagian yang paling stabil dari daerah pengendali mtDNA diamplifikasi secara in vitro dengan pasangan primer 577 dan 578. Produk PCR dipotong dengan lima macam enzim restriksi, yaitu Alu I, Hsp92 II, Hha I, Hpa II dan Rsa I yang menghasilkan tujuh haplotipe. Keragaman nukleotida antara haplotipe yang ditemukan di Jawa dan Sumatera (interlokasi) adalah 12.11 %. Angka itu jauh lebih besar dibanding keragaman nukleotida antar haplotipe di dalam Sumatera maupun Jawa (intralokasi) yang berturut-turut adalah 6.5% dan 3%.
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Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4