Judul: Isolation and Cloning of P5CS (pyrrolin-5-carboxylate synthetase) Gene from Rubber Plant
Abstrak: Rubber plant (Hevea brasiliensis) is one of the plantations that has important role in Indonesia. Molecular technique by using P5CS gene as molecular marker is selection method that faster and more accurate for producing rubber plant that compatible for marginal land. The aim of this research is to isolate and clone P5CS gene as a marker gene of drought resistant. Steps of this research are isolation of rubber plant DNA genome, isolation of P5CS gene from rubber plant by P5CS CS forward-P5CS CS reverse and P5CS start-P5CS stop primers, cloning into pGEM-T easy plasmid, isolation of plasmid DNA, sequencing, and analyzing DNA sequence. Isolation of rubber plant DNA genome is successfully proceed. Isolation of P5CS gene by using P5CS CS forward-P5CS CS reverse primer is not been successfully proceed, while isolation gene by using P5CS start-P5CS stop primer produce amplicon that has 2250 bp length. The confirmation of P5CS gene cloning by PCR technique using M13 forward-M13 reverse primer produce DNA that has 2450 bp length. The length of plasmid DNA that has been isolated is 5450 bp. The result of DNA sequence analysis by BLASTX shows that amplicon which has been isolated is not P5CS gene, but it has high homology (68%) with drought resistant gene.
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Judul: Analisis Keragaman Genetik Tanaman Karet Hasil Persilangan antara RRIM 600 dan PN 1546 dengan Menggunakan Teknik RAPD
Abstrak: Low productivity of rubber product had been the main problem in public rubber plantation in Indonesia. New assembly of excellent genotipe rubber plant requires 25-30 years. The more appropriate and accurate method is needed to early determine the new rubber plant clone. This research aimed to shows genetic variation of rubber plants-crossed product between RRIM 600 and PN 1546 with RAPD technique. Two DNA samples from rubber plants parent and 25 DNA samples from rubber plants-crossed products were isolated and amplified by using 16 mTcCIR combination primers and 60 RAPD combination primers. Amplification by using 16 mTcCIR combination primers produced a specific selected marker candidates, while amplification by using 60 RAPD primers produced polymorphism ribbon pattern in agarose gel. Phylogenetic tree was created based on the ribbon to identify the group and family distances of rubber plants-crossed product. Result showed that combination primer ‟d‟ and ‟i‟can be developed into specific marker candidates for RRIM 600. Phylogenetic tree showed that 25 rubber plants-crossed product have 10 high family groups with coeficient 89%. The family group showed that 25 rubber plant-crossed product had high diversity.
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Judul: Karakteristik Anatomi Skelet Kepala Buaya senyulong (Tomistoma schlegelii).
Abstrak: Buaya sepit atau senyulong (Tomistoma schlegelii) merupakan jenis buaya asli Indonesia yang berhabitat di sungai-sungai Sumatera, Kalimantan, dan juga pedalaman Sulawesi. Salah satu ciri khas buaya senyulong adalah bentuk moncong yang runcing dan sempit, namun hingga saat ini informasi mengenai struktur skelet kepala buaya senyulong sangat terbatas. Penelitian ini bertujuan untuk mempelajari karakteristik anatomi skelet kepala buaya senyulong yang dikatikan dengan fungsi dan perilakunya. Penelitian dilakukan secara eksploratif dengan melakukan pengamatan, pencatatan, dan pengambilan gambar dari skelet kepala buaya senyulong. Skelet kepala berukuran panjang 70 cm, lebar 29 cm, dan tingggi 17 cm dengan permukaan kasar pada os nasale, os frontale, os maxilla, os mandibula, os lacrimale, os jugale. Jumlah gigi pada buaya ini sebanyak 78 buah, terdiri dari 4 buah gigi premaxila, 16 buah gigi maxila, dan 19 buah gigi mandibula. Pada regio orbitalis ditemukan dua pasang fenestrae temporales yang berkembang baik. Fenestra supratemporale merupakan lubang oval yang membentang di sepanjang os parietale. Fenestra infratemporale berbentuk segitiga dan dibatasi oleh os squamosum, os postorbitale, os jugulare, dan os quadratum. Os mandibula hewan ini mempunyai symphysis mandibularis yang panjang yaitu garis yang melintang di corpus mandibulae. Secara fungsional ukuran symphysis mandibularis berkaitan dengan adaptasi hewan terhadap diet makan.
Keyword: kepala, moncong, Tomistoma schlegelii