Judul: Gambaran Restriction Fragment Length Polymorphism (Rflp)Gen Sitokrom B DNA Mitokondria Dari Sembilan Spesies Ikan Air Tawar Konsumsi
Abstrak: Penelitianinibertujuanuntukmengetahui datadasarfragmenrestriksi dari sembilanspesiesikanairtawarberdasarkanvariasigenetikyangterdapatpada daerahsitokrom bDNAmitokodriamelaluimetode PolymeraseChainReaction- RestrictionFragmentLengthPolymorphism (PCR-RFLP). Jenissampel yang digunakan berupajaringan ikan. Sebagaimediatransport danpreservasi ,sampel disimpandalamNaCl 4,5M yangmengandungDMSO 25%. EkstraksiDNA mitokondriadarijaringanmenggunakanmetodepresipitasiamoniumasetat, dilanjutkandenganamplifikasifragmenDNAmitokondriamelaluimetodePCR menggunakanprimeruniversalsitokrombL14841/H15149.Amplikonyang diperolehdarimetodePCR,selanjutnyadipotongdenganenzimrestriksi. PemotonganfragmenDNAmetodeRFLPmenggunakanenzimrestriksi endonuklease HinfI dan RsaI masing-masingselama2jamdan6jam. PemeriksaanfragmenDNAdenganelektroforesisgelagarose2%,laludilanjutkan analisishasilpenghitunganukuranfragmenrestriksidarisetiapspesiesdengan mengukurjarakantaramigrasiDNAsampeldenganmigrasiDNAladder.Hasil penelitianmenunjukkanbahwaPCRmenggunakanprimeruniversalsitokromb darikesembilanspesies ikandidapatkanamplikonsitokrombberukuran359 pasangbasa(pb).Amplikonsitokrombdarikesembilanspesiesikanmemiliki situsrestriksi HinfI danatau RsaI,kecualiamplikonsitokrombikansepat. Terdapatperbedaanukuranfragmenrestriksi(RFLP) hasilpemotonganbaik denganenzim HinfI maupun RsaI kecualipadaamplikonsitokrombikanpatin. Hasilpenelitianinidapatdisimpulkanbahwa metodePCRmenggunakanprimer universalsitokrombsertapemotonganfragmendenganenzimrestriksi HinfI dan RSAI dapatdigunakanuntukmengenali kesembilanspesiesikanairtawar. Katakunci:ikanairtawar,sitokromb,PCR,RFLP
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Judul: Restriction Fragment Length Polymorphism (RFLP) Gen Sitokrom B Dna Mitokondria Dari Delapan Spesies Burung
Abstrak: Penelitianinibertujuanuntuk mempelajari RestrictionFragmentLength Polymorphism (RFLP) delapanspesiesburunglokalberdasarkanfragmen Sitokrom b DNAmitokondria.Hasilyangdiperolehdapatdigunakansebagai dasarinformasiuntukmengenalispesiesburung.Delapansampeljaringanburung diperolehdisekitarBogordandisimpandalamlarutan NaCl4,5Myang mengandungDMSO25 %.DNAdiisolasidenganmetodepresipitasiamonium asetat.Primeruniversal SitokrombL14841/H15149 digunakanuntukamplifikasi fragmengen sitokrom b DNAmitokondriadenganmenggunakanmetode PolymeraseChainReaction (PCR) yangdilanjutkandenganelektroforesis.DNA hasilPCR(amplikon)kemudian dicerna (dipotong) denganmenggunakanenzim restriksiendonuklease Hinf I(2,5unit)dan Rsa I (20unit) masing-masing selama 2jamdan6jam.PenghitunganukuranDNA amplikon danfragmenhasil pemotongan dilakukandenganmengukurjarakantaramigrasiDNAsampel denganmigrasi DNAladder dalamgelagarose2%.Hasilpenelitianmenunjukkan bahwadelapanspesiesburunglokalmempunyaiamplikonyangberukuran 359pb,sertamempunyaiukuranfragmenrestriksi HinfI yangberbedakecuali bondolpekingdanmanyarjambul.Fragmenrestriksi Rsa I yangdihasilkanjuga berbedakecualimanyarjambuldandederukjawa,sertaayamkampungdan merbahcerukcuk.Berdasarkanhasilpenelitianinidapatdisimpulkanbahwa metodePCRmenggunakanprimeruniversalsitokrombsertapemotongan fragmendenganenzim restriksi HinfI dan atau Rsa I dapatdigunakanuntuk mengenali kedelapan spesiesburung. Katakunci: sitokromb, DNA mitokondria, HinfI, RsaI,burung
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Judul: Thermal Characteristics and Dynamics of Tropical Cyclone over Western North Pacific Ocean: A Case Study of Typhoon Songda (Chedeng) 2011
Abstrak: The frequent occasion of Tropical Cyclone (TC) over Western North Pacific (WNP) Ocean lies from 10˚N to 26˚N and 121˚E to 170˚E. Sources of TC development come from warm ocean temperature where latent heat is released during condensation processes and subsequently concentrated in the boundary layer. Relative humidity at 850-700 hPa is responsible to produce the most intense storm. Meanwhile, weak vertical wind shear between 850-200 hPa is one of the dynamical factors that lead to the development of TC. Pressure level of 850-200 hPa is chosen to minimize the thermodynamic effect in the lower troposphere. Typhoon Songda (Chedeng in the Philippines) or TSC lasted from May 18th to 30th, 2011 and strengthened into powerful Super Typhoon stage between May 24th and 27th. Long curving track and the exhibition of all sequences of TC life cycle, TSC is then considered as an ideal case of TC. The objective of the study with case of TSC is to investigate the role of thermal characteristics and dynamics on TC intensity. The research is carried out by using Advanced Research of Weather Research and Forecasting model (WRF ARW model) version 3.3. Two domains are employed in the model. The first domain situate from 02˚N to 40˚N and 118˚E to 149˚E and cover spatial resolution () of 33.1 km. The second domain situate from 09˚N to 27˚N and 119˚E to 132˚E with of 11 km. Timestep of the model () is 120 seconds. The model is supported by the following physical parameterization, i.e., Yonsei University Scheme (YSU) in the boundary layer, Kain-Fritsch scheme (KF) in the cumulus, and WRF Single-Moment 3-class scheme in the microphysics. Initial meteorological condition prior to the formation of the cyclone is generated using WRF ARW standard-release model. Sensitivity of the model output is obtained from a selected single-parameter change scenarios, namely, 10 % to 20 % decrease and increase of wind shear initial data at 850-200 hPa, and by 10 % to 20 % decrease of relative humidity initial data at 850-700 hPa. The result showed that the negative correlations between wind shear and intensity are found in wind shear scenarios. Wind shears of 10 ms-1 which lead to strengthening of the wind intensity in the early period of Typhoon development are found in wind shear scenarios. The effect of wind shear changes on wind intensity is statistically significant in the 20 % wind shear scenarios. During mature stage, weak wind intensity occurred when wind shear increase as given in the WRF standard-release and the 20 % decreasing scenario. Different situation is found in the 20 % increasing scenario where wind shear tend to weak and lead to strong wind intensity at this stage. Meanwhile, the reduction of moisture supply at 850-700 hPa resulting from 20 % decreasing relative humidity play a major role in the weakening of wind intensity and reduces mechanical energy of the cycle process as much as 300 J kg-1.
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