Judul: Analisis Polimorfisme DNA Tanaman Kunyit (Curcuma longa L.) dan Temulawak (Curcuma xanthorrhiza Roxb) Lokal Menggunakan Marka RAPD
Abstrak: Tanaman kunyit (Curcuma longa L.) dan temulawak (Curcuma xanthorrhiza Roxb) lokal Indonesia yang tersebar dan mudah ditemukan di wilayah Indonesia banyak digunakan sebagai obat tradisional. Kandungan kurkumin pada kedua tanaman tersebut memiliki banyak khasiat kesehatan, yaitu sebagai obat antiproliferatif dan antiinflamasi. Adanya polimorfisme DNA pada kedua tanaman tersebut di beberapa lokasi Pulau Jawa belum diidentifikasi. Oleh karena itu, perlu dilakukan analisis genetik untuk menentukan keragaman kedua tanaman tersebut. Salah satu teknik yang biasa digunakan untuk analisis keragaman genetik adalah PCR RAPD. Tujuan penelitian ini mengisolasi DNA tanaman kunyit dan temulawak dan menentukan pola genetik tanaman tersebut dengan PCR RAPD. PCR dilakukan dengan menggunakan 20 primer acak OPA, OPB, OPC, dan OPD (1-5). Ekstraksi DNA menggunakan metode modifikasi Zheng et al. 1995 pada 13 sampel daun temulawak dan kunyit. Hasil menunjukkan bahwa primer yang dapat mengamplifikasi DNA sampel, yaitu: primer OPA-1, OPA-3, OPA-4, OPC-1, OPC-2, OPC-4, dan OPD-4. Selain itu, hanya tiga sampel yang dapat teramplifikasi, yaitu: temulawak Wonogiri, temulawak Karang Anyar, dan kunyit Wonogiri. Sampel yang teramplifikasi memiliki pola genetik yang berbeda yang dihasilkan oleh setiap primer acak sehingga kedua puluh primer acak dapat digunakan sebagai marka untuk analisis fingerprint.
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Judul: Pola Keragaman Genetik Tanaman Kunyit (Curcuma longa Linn.) dan Temulawak (Curcuma xanthorrhiza Roxb.) Asal Pulau Jawa dengan PCR-RAPD
Abstrak: Turmeric (Curcuma longa Linn.) and Temulawak (Curcuma xanthorrhiza Roxb.) are Indonesian medicinal plants which nutritious for improving health. The pattern of genetic diversity and genetic relationship of these rhizome plants are not well known yet. The purpose of this research to analyze the pattern of genetic diversity and genetic relationship both plants using Polymerase Chain Reaction- Random Amplified Polymorhic DNA (PCR-RAPD). The sample used in this research include 6 turmeric plants from Bogor, Wonogiri, Karanganyar, Sukabumi and Ngawi; and 5 temulawak plants from Bogor, Wonogiri, Sragen, Karanganyar and Sukabumi. Stage of these research include DNA isolation; DNA amplification; and analysis pattern of genetic diversity and genetic relationship using NTSYS 2.02. The result of these research showed that isolation DNA was done successfully. The result of DNA isolation generally has a fairly good purity. The purity values of turmeric and temulawak in sequence were 1.093-1.417 and 1.044-1.544. The number of bands that arise from DNA amplification of turmeric and temulawak in sequence were 131 and 132 locus. The number of amplification bands mixed results and different sizes for each sample show the patterns of genetic diversity found in turmeric and temulawak plants. The closest genetic relationship showed by turmeric plants from Sukabumi and Ngawi; and temulawak plants from Bogor and Sukabumi. Genetic similarity coefficient ranged from turmeric plants and temulawak plants in sequence are 54%-92% and 55 %-83 %.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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