Judul: Identification of Variety and Association of Fatty Acids in NUDT8 Gene with Native Chicken and Broiler Crossbreed
Abstrak: Gen Nudix Hydrolase 8 (NUDT8) memiliki peran penting dalam pertumbuhan kanker, khususnya CoA terhidrolisis, enzim mitokondria dan banyak terekspresi dalam berbagai organ mitokondria seperti jantung, ginjal, hati, dan juga jaringan adiposa coklat (BTA). Penelitian ini bertujuan untuk mengidentifikasi gen NUDT8 dan hubungannya dengan sifat asam lemak. Jumlah sampel yang digunakan adalah 63 ekor ayam ras pedaging (KBKB). Identifikasi gen NUDT8 dianalisis menggunakan Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP). Hasil penelitian menunjukkan bahwa gen NUDT8 bersifat polimorfik yang ditunjukkan dengan adanya tiga genotipe pada gen NUDT8 (CC, CT, dan TT). Keragaman genotipik dari alel NUDT8 berada di Hardy Weinberg Equilibrium. Analisis SNP g.372602 C> T, gen NUDT8 diasosiasikan secara signifikan (P> 0,05) dengan Asam Lemak Jenuh (SFA), khususnya Asam Palmitat (C16: 0). Gen NUDT8 bersifat polimorfik dan dapat digunakan sebagai kandidat penanda komposisi asam lemak pada ayam ras broiler (KBKB)., Nudix Hydrolase 8 (NUDT8) gene had an important role in cancer growth, specifically hydrolyzed CoA, a mitochondrial enzyme and widely expressed in mitochondrial diverse organs as a heart, kidney, liver, and also brown adipose tissue (BTA). This research aimed to identify the NUDT8 gene and its association with fatty acid traits. The total sample used was 63 broiler cross-breed chickens (KBKB). Identification of NUDT8 gene were analyzed by using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP). The results showed that NUDT8 gene were polymorphic, which was indicated by presence of three genotypes in the NUDT8 (CC, CT, and TT) gene. The genotyipic diversity of the NUDT8 allels was in Hardy Weinberg Equilibrium. The analyses on SNP g.372602 C>T, NUDT8 gene was assosiated significantly (P>0.05) with Saturatted Fatty Acid (SFA), specially of Palmitat Acid (C16:0). NUDT8 gene is polymorphic and can be utilized as a candidate marker for fatty acids composition in broiler crossbreed chickens (KBKB).
Keyword: fatty acids, NUDT8 gene, PCR-RFLP

Judul: Keragaman Gen HSPG2 dan Asosiasinya terhadap Asam Lemak pada Ayam F2 Persilangan Kampung Broiler
Abstrak: Gen HSPG2 merupakan salah satu gen yang berperan penting dalam pemeliharaan dan fungsi jaringan adiposa dan serat otot. Penelitian ini bertujuan menganalisis keragaman gen HSPG2 serta asosiasinya dengan asam lemak pada ayam F2 persilangan kampung broiler. Total sampel yang digunakan sebanyak 62 sampel DNA ayam F2 persilangan kampung broiler. Identifikasi keragaman gen HSPG2|BsaHI dianalisis menggunakan PCR-RFLP. Produk amplifikasi memiliki panjang sebesar 356 bp. Gen HSPG2 bersifat polimorfik dengan menghasilkan 3 genotipe yaitu CC, CT, dan TT. Hasil chi-kuadrat menunjukkan gen HSPG2 berada dalam kesetimbangan Hardy-Weinberg. SNP gen HSPG2 posisi g.6574957 C>T pada genotipe CC berasosiasi (P<0,05) berasosiasi dengan asam lemak jenuh yaitu asam laurat (C12:0); asam palmitat (C16:0), asam lemak tidak jenuh tunggal yaitu asam oleat (C18:1n9c), asam lemak tidak jenuh ganda yaitu asam eikosedoneat (C20:2) dan total asam lemak. SNP gen HSPG2 disimpulkan berpotensi digunakan sebagai penanda genetik dalam program seleksi yang menghasilkan daging ayam dengan kandungan asam lemak tak jenuh tinggi.
Keyword: asam lemak, ayam, Gen HSPG2, PCR-RFLP, Nadira Andarini Siregar

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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