Judul: Analysis of Characteristic Working from Home, Subjective Well-Being and Outcomes Working from Home at PT. Asuransi Rama Satria Wibawa Jakarta
Abstrak: Penerapan kebijakan WFH bukanlah suatu metode bekerja di Indonesia, melainkan upaya mitigasi penyebaran COVID-19. PT. Asuransi Rama Satria Wibawa merupakan salah satu perusahaan yang menerapkan kebijakan WFH dan karyawan dituntut untuk tetap menjaga kinerjanya. Selain itu, subjective well-being bagi karyawan yang menjalankan WFH juga perlu diperhatikan dalam meningkatkan outcomes WFH. Penelitian ini bertujuan untuk mengindentifikasi karakteristik responden, menganalisis persepsi characteristic WFH, subjective well-being dan outcomes WFH serta pengaruh characteristic WFH dan subjective well-being terhadap outcomes WFH. Metode penarikan sampel menggunakan sensus dengan jumlah responden sebanyak 46 orang. Penelitian ini menggunakan metode analisis deskriptif dan SEM-PLS. Karyawan pada PT. Asuransi Rama Satria Wibawa didominasi oleh karyawan laki-laki sebanyak 33 orang, rentang usia 31-40 tahun sebanyak 16 orang, berstatus menikah sebanyak 32 orang, lulusan Strata Satu (S1) sebanyak 23 orang dan masa kerja 2-5 tahun sebanyak 30 orang. Persepsi karyawan terhadap characteristic WFH, subjective well-being dan outcomes WFH mayoraitas baik. Hasil analisis SEM-PLS menunjukkan bahwa characteristic WFH dan subjective well-being berpengaruh positif terhadap outcomes WFH., The implementation of WFH policies is not an organizational culture in Indonesia but rather an effort to mitigate the spread of COVID-19. PT. Asuransi Rama Satria Wibawa is one company that implements WFH policies, and employees are required to maintain their performance. Besides, subjective well-being for employees who practice WFH also needs to be considered in improving WFH outcomes. This study aims to identify the characteristic of respondents, analyze the perceptions of WFH characteristics, subjective well-being, and WFH outcomes and the influence of WFH characteristics and subjective well-being on WFH outcomes. The sampling method uses cencus with 46 total respondents. This research uses a descriptive analysis method and SEM-PLS. Employees at PT. Rama Satria Wibawa Insurance is dominated by 33 male employees, 16 people from 31-40 years old, 32 people were married, 23 people graduated from S1 and 30 people working periods of 2-5 years. Employees' perceptions of WFH characteristics, subjective well-being, and WFH outcomes are generally good. The SEM-PLS analysis results show that WFH characteristics and subjective well-being have a positive effect on WFH outcomes.
Keyword: characteristic WFH, outcomes WFH, SEM-PLS, subjective well-being

Judul: The Influence of Motivation on  Employee’s Performance during WFH Throughout the Covid-19 Pandemic
Abstrak: Kebijakan Work from Home (WFH) merupakan salah satu upaya mengurangi tingkat kasus Covid-19. Selama WFH karyawan hanya dapat melaksanakan tugasnya dan kegiatannya di dalam rumah. Salah satu cara adalah tetap memotivasi karyawan agar dapat melaksanakan pekerjaannya dengan baik, untuk itu perlu diketahui faktor-faktor yang dapat memberikan motivasi selama WFH. Tujuan dari penelitian ini adalah mengidentifikasi karakteristik karyawan di DKI Jakarta yang melaksanakan WFH, menganalisis persepsi karyawan terhadap motivasi dan kinerja karyawan, dan menganalisis pengaruh motivasi terhadap kinerja karyawan selama melaksanakan WFH. Jenis data pada penelitian ini adalah data kuantitatif dan sampel dari penelitian ini diambil menggunakan teknik purposive sampling. Responden penelitian ini adalah 100 orang karyawan. Metode yang digunakan adalah metode analisis deskriptif dan analisis SEM-PLS. Hasil dari penelitian ini menunjukkan bahwa motivasi memiliki pengaruh yang positif dan signifikan terhadap kinerja karyawan., Work from Home (WFH) policy as one way to overcome the rate of Covid 19 cases. Employee are required to do their activity either working or basic daily activities at home. The organization should take a look at the problem closely and give work motivation to the employee to increase their performance. The purposes of this study are to identify the characteristics of employees in DKI Jakarta during WFH, determine the employee’s perception of motivation and employee’s performance, analyze the influence of motivation on the employee’s performance in DKI Jakarta during WFH. The type of data in this study is quantitative and the respondent in this study are determinate by purposive sampling. The respondent obtained were 100 employees. The method of analysis in this study were descriptive analysis method and SEM-PLS analysis. The results of this study indicate the influence of motivation on employee performance was accepted that motivation has influence the employee performance during WFH activity throughout the Covid-19 pandemic with positive and significant effect.
Keyword: Employee Performance, Motivation, Work from Home

Judul: Gene Editing  of Two Cyanide Synthesis-related Genes in Cassava using CRISPR/Cas9
Abstrak: Cassava (Manihot esculenta) contain toxic quantities of cyanogenic glucoside. Reducing the potentially toxic cyanogenic glucoside levels in cassava could be achieved by gene editing through Clustered Regularly Interspaced Short Palindromic Repeats – CRISPR associated protein 9 (CRISPR/Cas9), a high- precision genome editing technique in plants. Two cassava UDP- glucosyltransferase (UGT) paralogs, UGT85K4 and UGT85K5, are the genes catalyzing the synthesis of cyanogenic glucosides in plants. The study aimed to construct the CRISPR/Cas9 recombinant vector carrying single guide RNA (sgRNA) to edit UGT85K4 and UGT85K5 genes, and transform sgRNA CRISPR/Cas9 construct into KU50 cassava calli. Five sgRNAs were designed, considering the on/off target efficiency score and GC content. Those sgRNAs were ligated into pRGEB32 plasmid and introduced to competent Escherichia coli DH5a. The PCR colony and DNA sequencing were performed to verify the sgRNA insert. The amplicons showed the expected band size of recombinant vector (180 bp). Based on sequencing results, only one sgRNA was successfully ligated into the plasmid that will be used to edit the UGT85K5 gene. The sgRNA CRISPR/Cas9 construct in pRGEB32 plasmid was successfully transformed into Agrobacterium tumefaciens and introduced into KU50 cassava calli through co-cultivation. The overgrowth of Agrobacterium occurred after the co-cultivation phase.
Keyword: CRISPR/Cas9, Cyanogenic glucoside, Gene editing, sgRNA