Judul: Perbandingan Pola Pita Amplifikasi DNA Daun, Bunga, dan Buah Kelapa Sawit Normal dan Abnormal.
Abstrak: Kelapa sawit dimanfaatkan sebagai bahan baku industri minyak goreng, makanan, kosmetik, sabun, lilin, logam, serta biodiesel. Permintaan Crude Palm Oil (CPO) yang semakin meningkat kurang bisa dipenuhi sehingga perbanyakan bibit unggul kelapa sawit dilakukan dengan kultur jaringan. Namun teknik ini menghasilkan tanaman kelapa sawit yang abnormal, sehingga diperlukan penanda molekuler yang mampu mendeteksi abnormalitas secara dini. Penelitian bertujuan mengetahui perbedaan genotip daun, bunga, dan buah dari tanaman kelapa sawit normal dan abnormal, berdasarkan perbedaan pola pita amplifikasi DNA sampel terhadap primer acak menggunakan teknik amplifikasi DNA. Hasil penelitian menunjukkan perbedaan pita amplifikasi yang muncul pada sampel DNA bunga abnormal dengan primer OPA 8, DNA buah normal dengan primer OPB 2, dan DNA buah abnormal (primer OPA 18, OPA 20, OPB 2, dan OPB 9). Fragmen DNA yang berbeda kemudian diisolasi dan disekuensing. Sekuen basa yang berhasil dibaca hanya DNA buah abnormal dengan primer OPB 9. Analisis hasil sekuen dilakukan dengan program Basic Local Alignment and Search Tool for Nucleotida (BLAST) namun tidak menunjukkan homologi yang tinggi dengan gen dalam bank gen. Hasil BLAST juga tidak menghasilkan urutan basa yang ada pada suatu gen penyandi tertentu dalam tanaman kelapa sawit.
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Judul: Analisis genotipe normal dan abnormal pada klon kelapa sawit (Elaeis guineensis Jacq.) dengan RAPD
Abstrak: The formation of flower and fruit abnormalities in oil palm still unclear. The aim of this study is to analyze the genetic similarities, grouping among normal and abnormal genotypes, and to obtained a specific DNA band for abnormalities by RAPD analysis. Plant materials have been used i.e. MK152, Mk203, MK209, and MK212 (normaVabnormal and male flowers), while clones MK104 and MK176 (normal and abnormal). Amplification of DNA samples have been done with 15 random primers. Genetic similarities and phenogram were analyzed with NTSYS-pc.
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Judul: A Continuous Vaccination Strategy in the SVIR Epidemic Model
Abstrak: Vaccination is conducted to minimize the spread of a disease. The vaccination is usually done several times within a fixed time interval. In the SVIR model, it is assumed that individuals do not get immediate immunity following a vaccination program. So according to process of vaccination on SVIR model, there are two strategies, i.e. the continuous vaccination strategy (CVS) and the pulse vaccination strategy (PVS). In this study, only the CVS strategy in epidemic model SVIR is considered. Results of the study indicate that dynamics of the CVS system depends on the basic reproductive number. If the basic reproductive number is less than one, then the disease-free fixed point is asymptotically stable. It means that the disease will eventually disappear from the population. Conversely, if the basic reproductive number is greater than one, then the endemic fixed point is asymptotically stable, which means that the disease will remain exist in the population. Simulation result shows that the vaccination would minimize the spread of disease by reducing the basic reproductive number. If the time for the vaccinated recipients to obtain the immunity, as well as a possibility of vaccinated recipients to be infected are neglected, then the disease will be eradicated. This condition is called an over-evaluating effect of vaccination.
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