Judul: Application  of  Recombinant  Giant  Grouper  Growth  Hormone on Glass eel With Different Immersion Dose.
Abstrak: Penelitian dilakukan untuk menentukan dosis optimum hormon pertumbuhan rekombinan ikan kerapu kertang (rElHP) yang menghasilkan pertumbuhan tertinggi pada ikan sidat stadia glass eel melalui metode perendaman. Penelitian ini menggunakan 6 perlakuan dengan 3 ulangan. Dosis rElHP yang diberikan, yaitu 0 mg/L, 3 mg/L, 6 mg/L, 9 mg/L, 12 mg/L dan kontrol tanpa rElHP. rElHP dilarutkan dalam 200 mL air yang mengandung NaCl 0,6% dan serum albumin sapi 0,01%. Ikan sebanyak 50 ekor diberi kejutan salinitas (NaCl 3%) selama 2 menit, kemudian direndam dalam larutan rElHP selama 2 jam. Benih dipelihara selama 8 minggu dan diberi pakan berupa cacing sutera secara ad libitum. Hasil penelitian menunjukkan bahwa perlakuan perendaman 3 mg/L memberikan pertumbuhan bobot, biomassa, dan kelangsungan hidup tertinggi. Ikan perlakuan 3 mg/L memiliki biomassa sekitar 28,0% lebih tinggi, dan kelangsungan hidup sekitar 15,2% lebih tinggi dibandingkan dengan kontrol. Rerata panjang total tubuh ikan sidat antar perlakuan adalah tidak berbeda. Dengan demikian, perendaman rElHP dosis 3 mg/L adalah optimum dalam meningkatkan pertumbuhan bobot dan kelangsungan hidup benih ikan sidat.
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Judul: Respons benih ikan sidat terhadap hormon pertumbuhan rekombinan ikan kerapu kertang melalui perendaman dan oral
Abstrak: This study was aimed to determine an appropriate administration method and observe the response of eel fingerling on recombinant giant grouper growth hormone (rElGH). First study aimed to determine the immersion dose of rElGH to increase growth of eel juvenile (glass eel). After shock salinity treatment (NaCl 3% for 2 min), glass eel were immersed in water containing 0.9% NaCl, 0.01% bovine serum albumin, and different of dose of rElGH (0, 0.12, 1.2, 12 and 120 mg/L). The results showed that higher in growth body weight was obtained in treatment 12 mg/L (101.2±0.8), by increment of about 37.4% higher compared to that of control (72.93±1.8). Second study was aimed to determine the dose of rElGH mixed in artificial diet to increase growth of eel elver. HP55-coated rElGH was mixed to diet at different doses (0, 0.3, 3, and 30 mg/kg). Elver was fed on diet containing rElGH twice daily, 6% fish biomass. The result showed that higher in growth body weight was obtained in treatment 30 mg/kg (60.18±1.38), by increment of about 65.7% compared to control (36.32±0.97). Third study was aimed to compared of three administration method, namely immersion, oral, and combination of immersion and oral. The dosage of rElGH by immersion (12 mg/L) and oral administration (30 mg/kg feed) was obtained in previous study. Fish were maintained in 23-L glass aquaria for 120 days, at density of 45 fish per aquarium. The results showed that higher (P<0.05) biomass of harvest (73.68±2.07 g) was obtained in immersion and oral combination treatment, by increment of about 102.9% compared to control (36.32±0.97 g). Administration of rElGH also improved protein and lipid retentions by 142.5%, and 720% compared to that of control, respectively (P<0.05). In addition, rElGH treatment increased appetite, while artificial feed conversion ratio (4.75) was lower (P<0.05) by 55.15% compared to that of control (7.37). Combination of rElGH immersion and oral administrations also increased hepatosomatic index, reduced ammonia excretion and increased insuline-like growth factor-1/IGF-1 gene expression. Thus, rElGH administration via immersion and oral was a proper method to improve performance of eel fingerling, and application of rElGH can be useful to increase aquaculture production.
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Judul: Penggunaan Teorema Homeomorphy 2-Manifold dan Teorema Euler Poincare pada Torus 𝑇�� dan Simplicial Complex 𝐾��
Abstrak: Two dimensional topological spaces are said to be homeomorphic if they have the same topological invariant, where one of topological invariant used is an Euler characteristic. Homeomorphy 2-Manifold’s Theorem and Euler Poincare’s Theorem are used to distinguish two topological spaces. Homeomorphy 2-Manifold’s Theorem uses Euler characteristic to identify two dimensional topological spaces. Euler Poincare’s Theorem is an alternative way to find Euler characteristic with Betti number, which is topological invariant as well. The objective of this paper is to investigate the homeomorphism of torus 𝑇����� and simplicial complex 𝐾�����. Topological space torus and simplicial complex 𝐾����� have the same Euler characteristic, which is two. Based on Homeomorphy 2-Manifold’s Theorem topological space torus 𝑇����� and simplicial complex 𝐾����� is homeomorphic.
Keyword: simplicial, torus, Euler, homeomorphism, topology