Judul: Pemodelan Spasial Konservasi Lingkungan Dengan Pendekatan Sosial Ekonomi Masyarakat Di Kota Ambon
Abstrak: The increase of population may cause a domino effect in all aspects of life, especially in urban areas. The presence of vegetation cover will also threatened when the population continues to increase and subsequently may cause various effects of the erosion, flooding, landslides, and so on. This study, was performed to establish the Environmental Conservation Index (IKL) in Ambon to measure sustainability of the environment. The study was conducted from January to March 2012 in the Ambon City. Data collection was derived form face-to-face interviews with 440 respondents and secondary data compilation. The data analysis includes (a) weight determination; (b) score establishment; and (c) environmental conservation index development. The IKL models developed in this study is: IKL Urban = 0,127*Tl_skor + 0,095*Slop_skor + 0,175*Psm_skor + 0,238*Pdk_skor + 0206*Pddk_skor + 0,159*Pdptn_skor. The study found that 30.8 % of the Ambon City is categorized into conservative; 49.6% belong to less conservative categories, and into 19.7% extriemely conservative. Major proportion of the public community provides positive perseption to the importance of environment problem in Ambon City, where 77% of the activities involved in environment conservation. About 50% of the community expresses their satisfication the performance of the to government on management environmental problem.
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Judul: Kajian Spasial Kebutuhan Hutan Kota Berbasis Hidrologi di Kota Ambon
Abstrak: Air merupakan kebutuhan dasar bagi seluruh makhluk hidup. Tumbuhan berperan penting dalam proses pengaturan air. Di daerah perkotaan dengan segala perkembangannya, keberadaan air sangat diperlukan, namun pembangunan wilayah kota justru membuat lahan bervegetasi untuk penyerapan air semakin sempit. Pengembangan hutan kota merupakan rancangan ideal untuk pemenuhan kebutuhan ruang terbuka hijau di wilayah kota, terutama untuk fungsi peresapan air yang nantinya akan meningkatkan pasokan air tanah. Penelitian ini ditujukan untuk mengestimasi kebutuhan luas hutan kota di Kota Ambon khususnya untuk penyediaan air, dan untuk memberikan informasi bagi suatu perencanaan pengembangan RTH. Penelitian dilaksanakan mulai bulan Februari 2009 – April 2009 di Kota Ambon. Pengumpulan data dilakukan melalui observasi, wawancara dengan instansi terkait dan masyarakat, serta studi pustaka. Data yang dibutuhkan antara lain: jumlah dan laju pertumbuhan penduduk, industri dan ternak, potensi air tanah, kapasitas suplai air PDAM,dan data-data spasial berupa peta tutupan lahan, peta administrasi, dan peta jenis tanah. Keseluruhan data tersebut diolah dan dianalisis sehingga diperoleh informasi kebutuhan air total, suplai air total, kapasitas hutan kota dalam menyerap air, serta luas hutan kota optimal. Hasil penelitian menunjukkan bahwa kebutuhan hutan kota selalu meningkat dari tahun ke tahun. Perkiraan kebutuhan hutan kota di Kota Ambon untuk tahun 2010 yaitu 20.286.93 ha, tahun 2015 sebesar 22.691,32 ha, tahun 2020 sebesar 25.398 ha, dan tahun 2025 sebesar 28.444,93 ha. Titik keseimbangan antara kebutuhan dan ketersediaan hutan kota terdapat di pertengahan tahun 2012. Pada tahun tersebut diperkirakan jumlah penduduk Kota Ambon akan mencapai 309.065 jiwa dengan kebutuhan air sebesar 15.623.991 m3/tahun.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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