Judul: Kajian myelositomatosis pada ayam broiler sebagai imunosupresor
Abstrak: Myelocytomatosis or myeloid leucosis (ML) is a kind of neoplastic disease caused by Avian leucosis virus subtype J (ALV–J) infection, which often found in chickens worldwide. Myelocytomatosis in chickens cause reduced productivity and increased mortality in flocks. The aim of this study was to make pathological assessment of ML occurred in a broiler farm. Increased mortality with breathing difficulty (dyspnoea) were noted in broiler flocks aging 7 and 14 days. Afterwards necropsy was performed and organs were sampled for histopathological examinations. All organs were embedded in paraffin, sectioned at 5 μm, and stained with hematoxylin and eosin (for morphology), periodic acid–Schiff for carbohydrates, and Masson trichrome for collagens. Histopathological findings of ML included scattered myeloid cells depositions in the interstitium and perivascular area of kidney, liver, lung, and heart. Additionally, high percentage (>70%) of myeloid cells were found in the bone marrow. In addition to ML in various organs, there were invasive granulomatous pneumonia comprises of poorly demarcated fibrous bands, and septate fungal hyphae which consistent with the morphology of Aspergillus spp. Myelocytomatosis were also detected in the breeder flocks aging 29 weeks, notably in liver and ovaries. These findings suggest that vertical ML transmission may cause immunosupression which lead to invasive granulomatous lesion. Invasive granulomatous pneumonia with ML has never been documented in poultry.
Keyword: Bogor Agricultural University (IPB), myelocytomatosis., invasive granulomatous pneumonia, broiler, aspergillosis

Judul: Studi Kasus Patomorfologi Inclusion Body Hepatitis Pada Ayam Broiler Imunosupresif
Abstrak: Inclusion Body Hepatitis (IBH) yang disebabkan oleh infeksi adenovirus pada unggas sudah dilaporkan di berbagai belahan dunia. Akan tetapi, penyakit ini sangat jarang dilaporkan di Indonesia. Tulisan ini mendeskripsikan gambaran patologi IBH yang terjadi pada ayam broiler. Ayam broiler berumur tujuh hari yang menunjukkan gejala-gejala non-spesifik seperti lemah, lesu, dan penurunan nafsu makan, dibawa ke Laboratorium Patologi, Departemen Klinik, Reproduksi dan Patologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor untuk dinekropsi. Gambaran makroskopik utama yang ditemukan adalah hati yang bengkak, rapuh, dan pucat disertai bercak perdarahan titik dan ekimosa serta Hidroperikardium. Sampel organ kemudian diambil untuk pemeriksaan histopatologi. Selanjutnya, organ ditanam dalam parafin dan diwarnai dengan hematoksilin-eosin (HE). Pada pemeriksaan histopatologi hati ditemukan multifokal nekrosis yang dikelilingi oleh infiltrasi sel radang mononuklear dan Ferdarahan yang mengindikasikan hepatitis nekrotikan; dan pada inti sel hepatosit banyak ditemukan badan inklusi eosinofilik dan basofilik yang sama dengan badan inklusi adenovirus. Selain lesi yang ditemukan di hati, ditemukan juga atropi bursa Fabrisius yang dicirikan oleh apoptosis sel-sel limfoid, edema terstitial, dan pembentukan kista pada epitel plika. Pada berbagai organ ditemukan pula lesio limfomatous yang diduga disebabkan infeksi Marek dengan ari banyaknya kumpulan sel-sel limfoid berukuran kecil sampai besar pada esofagus, proventrikulus, limpa, otak, dan iris. Invasi sel tumor Marek ke jaringan limpa menyebabkan deplesi pulpa putih limpa. Pada kasus ini lesi nefrosis dan kardiomiopati ringan merupakan lesi minor. Adanya deplesi folikel limfoid bursa fabrisius dan limpa merupakan lesi kronis yang mengindikasikan imunosupresi. Berdasarkan deskripsi di atas, munculnya kasus IBH kemungkinan didahului imunosupresi sebagai faktor predisposisi. Pada kasus ini, sumber adenovirus tidak dapat diketahui asalnya.
Keyword: Inclusion Body Hepatitis (IBH), Patomorphology, Depletion of lymphoid follicles, Immunosuppresion

Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4