Judul: Hama dan Penyakit Jambu Air (Syzygium samarangense (Blume) Merr. & L.M. Perry) di Kabupaten Demak, Jawa Tengah.
Abstrak: Water apple is a fruit icon of Demak Regency. Pests and diseases are major problems in water apple cultivation. This research aims to inventory the main pests and diseases of water apple in Demak Regency, Central Java. Research was conducted in three steps: field observation and collection, species identification in the laboratory, and interview water apple farmers. Field observation was conducted in four water apple plantations in Bintoro district. Ten water apple trees were sampled diagonally from each plantation and observation was conducted on four twigs that were determinated based on cardinal direction: west, east, north, and south. Interview farmer was based on questionaire specially designed to reveal farmer’s identity, cultivation techniques, pests and diseases management in water apple plantation. There were some pests found such as spiralling white fly (Aleurodicus dispersus), white moth cicada (Sanurus indecora), fruit flies (Bactrocera albistrigata), leaf-roller weevils (Apoderus trinotatus), leaf borer (Lepidoptera: Gracillaridae), leaf folder (Lepidoptera: Tortricidae), and shoot roller (Lepidoptera: Tortricidae). The diseases were found such as red rust (Cephaleuros sp.), sooty mold (Capnodium sp.), pink disease (Corticium salmonicolor), and anthracnose (Gloeosporium sp.).
Keyword: Density, identification, inventory, plant damage, severity

Judul: Validation of Microsatellite Markers on Several Water Apple (Syzygium aqueum (Brum.f.) Alston)
Abstrak: Jambu air (Syzygium aqueum (Burm.f.) Alston) merupakan spesies yang masuk ke dalam famili Myrtaceae. Famili ini memiliki jumlah spesies yang banyak sehingga sulit untuk menganalisis perbedaan antar spesies ataupun kultivar. Analisis dengan menggunakan parameter morfologi kurang akurat karena dipengaruhi lingkungan sehingga perlu dilakukan analisis secara molekuler menggunakan marka mikrosatelit. Penelitian ini bertujuan memvalidasi marka mikrosatelit pada beberapa kultivar jambu air. Percobaan ini dilakukan di Laboratorium Seed center, Kebun Percobaan Leuwikopo, Institut Pertanian Bogor, Dramaga, Bogor. Bahan penelitian ini terdiri dari 10 kultivar jambu air dan 10 marka mikrosatelit. Waktu penelitian dimulai dari bulan September 2022 hingga Mei 2023. Penelitian ini dimulai dari proses pengambilan sampel, ekstraksi DNA, Pengujian kualitas dan kuantitas DNA, amplifikasi DNA, hingga elektroforesis hasil amplifikasi. Hasil penelitian menunjukkan bahwa marka mikrosatelit yang digunakan mampu mengamplifikasi 60-100% kultivar jambu air. Semua marka/lokus dapat digunakan untuk analisis molekuler, namun hanya lokus P10 yang mampu membedakan genetik antar kultivar. Lokus P10 diduga merupakan lokus polimorfik., Water Apple (Syzygium aqueum (Burm.f.) Alston) is a species belonging to the family Myrtaceae. This family has a large number of species so it is difficult to analyze differences between species or cultivars. Analysis using morphological parameters is less accurate because it is influenced by the environment, so it is necessary to carry out molecular analysis using microsatellite markers. This study aims to validate microsatellite markers on several water apple. This experiment was conducted at the Seed center Laboratory, Leuwikopo Experimental Garden, Bogor Agricultural University, Dramaga, Bogor. The research material consisted of 10 guava cultivars and 10 microsatellite markers. The study time is from September 2022 to May 2023. This research starts from the sampling process, DNA extraction, DNA quality and quantity testing, DNA amplification, to electrophoresis of amplification results. The results showed that the microsatellite markers used was able to amplify 60-100% of the water apple cultivars. All markers/loci can be used for molecular analysis, but only P10 loci capable of distinguishing genetically between cultivars. The P10 locus is thought to be a polymorphic locus.
Keyword: Amplifikasi, Asam Nukleat, Elektroforesis, Genetik, Myrtaceae

Judul: Separation of Neodymium from U3Si2/Al Fuel Plate for Burn-Up Determination
Abstrak: Neodymium is a common fission product produced in nuclear fission reactions. Neodymium in this study was chosen as a burn-up indicator for U3Si2/Al fuel element plates because the neodymium isotope is a stable fission product with a fairly high yield. Some procedures were carried out to separate neodymium, starting with the separation of cesium isotopes (137Cs, 134Cs) through the precipitation method, then separation of uranium isotopes (235U, 238U) and plutonium isotopes (239Pu, 238Pu) with Dowex 1x8 ion exchange resin, and separation of neodymium (Nd), samarium (Sm), as well as cerium (Ce) with Dowex 1x4 ion exchange resin. The Cs recovery reached 99.95%. The U and Pu isotopes resulting from the separation are subjected to an electrodeposition process and the weight of each isotope can be measured with an alpha spectrometer. The separation results of Nd from Sm and Ce have been proven using an XRF spectrometer. The neodymium isotopes separation was corrected using the standard addition method, and an average total Nd content of 2.4 ppm was obtained in the PEB U3Si2/Al bottom solution. It is hoped that this separation method can be applied in determining burn-up of nuclear fuel.
Keyword: neodymium, burn-up indicator, U3Si2/Al fuel plate