Judul: Rizobakteria Pseudomonas sp. pemacu tumbuh toleran kekeringan dan aplikasinya pada tanaman jagung di rumah kaca
Abstrak: Kekeringan dapat menurunkan pertumbuhan dan produktivitas tanaman. Pseudomonas sp. merupakan bakteri rizosfer yang dapat memacu pertumbuhan tanaman (Plant Growth Promotion Rhizobacteria) karena menghasilkan hormon pertumbuhan IAA. Selain itu, Pseudomonas juga mesekresikan eksopolisakarida. Eksopolisakarida merupakan senyawa ekstraseluler yang mampu mempertahankan retensi air di sekitar rizosfer dan juga dapat melekat dengan baik pada rizosfer. Sebanyak sembilan isolat Pseudomonas CRB, dari 34 isolat Pseudomonas hasil penelitian sebelumnya, diketahui mampu meningkatkan pertumbuhan panjang akar dan panjang batang kecambah jagung. Isolat tersebut adalah CRB 4, 10, 19, 23, 24, 34, 47, 71, dan 98. Hasil uji eksopolisakarida juga menunjukkan bahwa semua isolat tersebut menghasilkan eksopolisakarida sebanyak 3.39.0 mg/ml. Enam dari isolat tersebut, yakni CRB 4, 10, 19, 23, 47, dan 98 dibuat formulanya dalam bahan pembawa gambut. Isolat ini terpilih karena mampu tumbuh pada media dengan tekanan osmotik -2 Mpa dengan menghasilkan Optical Density≥ 0.4.Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan dua faktor (cekaman dan formula). Faktor cekaman terdiri atas dua level, yaitu kering dan basah, sedangkan formula terdiri atas enam level yaitu Formula F1-F6 dengan 1 kontrol. Kombinasi faktor tersebut diulang sebanyak tiga kali. Hasil pengujian menunjukkan bahwa formula F1 yang mengandung CRB 4 meningkatkan berat basah akar tanaman jagung pada kondisi kering (kadar air 20,5%).
Keyword: Drought, Pseudomonas, Enhance growth, Exopolysaccharide, Formulation, Maize

Judul: Penapisan Rizobakteria Toleran Kekeringan Pemacu Tumbuh Tanaman Kedelai dan Aplikasinya di Rumah Kaca
Abstrak: Drought stress can reduce the productivity of crops. Therefore, strategies to promote growth of plants that can grow well in drought conditions is required. One of the way is by using the plant growth promoting rhizobacteria (PGPR). The aim of this study was to screen rhizobacteria in drought stress and apply them on soybean in greenhouse. A total of 27 rhizobacteria consisted of 14 Pseudomonas sp. (Crb), 11 Bacillus sp. (Cr), and 2 Bradyrhizobium japonicum. (Bj) was screened on drought conditions using media supplemented with Polythylene Glycol (PEG) 6000 then quantitatively tested for exopolysaccharide product. Selected bacteria were tested for antagonistic assay tested before combining them in a package formula in the peat. Bacteria were grown in media skim milk and molasses then inoculated into 50 g of peat soil. The formula were tested for the viability and effectiveness on soybeans in green house. Total of 17 bacteria were successfully screened and all grow well at low osmotic potential (-2 Mpa) medium and produced exopolysaccharide ranging 1.7-8.0 mg/ml. Based on the exopolysaccharide produced and antagonist test, five packages of formula were selected. Viability of five formulas were stable until the three months of storage period. Application of the formulas for soybean in green house, formula F4 showed significant effect on plant height, shoot and root fresh weight and root dry weight in both wet and dry conditions.
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Judul: Morfometri Organ Hati dan Kantung Empedu Kucing Kampung (Felis catus) dengan Teknik Ultrasonografi Dua Dimensi
Abstrak: The aims of this study was observed and determined the sonogram and size of liver and gallbladder in domestic cats using ultrasound examination. Animals used were 5 healthy male cats, aged between 3-5 years with a body weight between 3-4 kg. Examination was performed using a 7.5 MHz linear transducer. Sonogram showed that the liver parenchym was homogeneous and hypoechoic. On the transverse position of the transducer, the thickness of the medial, right, and left liver was 3.73 ± 0.10 cm, 3.75 ± 0.13 cm, and 3.72 ± 0.11 cm, respectively. There were three thickness values measured on the sagital position of the transduser, namely at the widest, medium and shortest distance to the diafragma. Hepatic vein sonogram showed that the lumen was anechoic and the outer line was hyperechoic. Hepatic vein had an internal diameter of 0.18 ± 0.01 cm on the transverse transducer position and 0.17 ± 0.01 cm on the sagital transducer position. Sonogram of the portal vein showed that the lumen was anechoic and the outside line was hyperechoic. Portal vein measured in the transverse transducer position showed an internal diameter of 0.17 ± 0.01 cm, external diameter of 0.30 ± 0.02 cm and wall thickness of 0.07 ± 0.01 cm. Portal vein measured in the sagital transducer position showed an internal diameter of 0.17 ± 0.01 cm, external diameter of 0.31 ± 0.01 cm and wall thickness of 0.07 ± 0.01 cm. Sonogram of the gallbladder showed an oval or round, and smooth shape. The lumen was anechoic and the wall was hyperechoic. The wall thickness was 0.08 ± 0.01 cm. Morphometry liver and gallbladder of an Indonesian Domestic House Cat (Felis catus) was clearly observed and determined using two dimensional ultrasonography.
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