Judul: Land Cover Change Using High-Resolution Imagery in  Nagrak Sub-district, Sukabumi District
Abstrak: Perubahan penutupan lahan merupakan isu secara global yang disebabkan oleh faktor sosial, ekonomi, pertumbuhan penduduk, dan kebijakan pemerintah. Taman Nasional Gunung Gede Pangrango merupakan salah satu kawasan yang mengalami perubahan penutupan lahan akibat kebijakan pemerintah. Wilayah perluasan yang signifikan berada di Kecamatan Nagrak. Selain itu, di Kecamatan Nagrak dalam beberapa tahun terakhir terjadi peningkatan penduduk, sehingga perlu dilakukan monitoring. Monitoring dilakukan dengan memanfaatkan teknologi penginderaan jauh. Penelitian ini bertujuan mengklasifikasi kelas penutupan lahan, menganalisis perbandingan metode, dan perubahan penutupan lahan dari tahun 2017 sampai tahun 2022. Metode yang digunakan diantaranya, klasifikasi visual dan digital. Klasifikasi visual menghasilkan sembilan kelas dengan uji akurasi 94%. Klasifikasi digital menggunakan random forest menghasilkan uji akurasi yang lebih rendah dari visual yaitu 89%. Hasil klasifikasi visual dan digital digabungkan dengan menu identity yang bertujuan untuk menghasilkan kelas penutupan lahan lebih baik dan akurat. Perubahan penutupan lahan tahun 2017–2022 dianalisis berdasarkan pengaruh perubahan penutupan lahan dari segi ekologi. Perubahan yang terjadi ke arah positif sebesar 192,36 ha atau 2,79% sementara ke arah negatif sebesar 33,17 ha atau 0,48%.
Keyword: citra SPOT 6/7, digitasi on-screen, penutupan lahan, random forest

Judul: Land Using Sentinel-2 Imagery in Sukamakmur District, Bogor Regency from 2018-2023
Abstrak: Pertumbuhan penduduk yang pesat mengakibatkan tingkat kebutuhan masyarakat terhadap lahan semakin meningkat sehingga menyebabkan terjadinya perubahan tutupan lahan. Penelitian ini bertujuan untuk mengidentifikasi dan menganalisis perubahan tutupan lahan di Kecamatan Sukamakmur Kabupaten Bogor menggunakan Citra Sentinel-2 tahun 2018–2023. Terdapat 9 kelas tutupan lahan yaitu hutan, sawah, kebun campuran, pertanian lahan kering, pemukiman, lahan terbuka tanah, semak, sungai, dan danau. Kelas tutupan lahan yang mengalami peningkatan adalah tutupan lahan hutan (83,40 ha), pertanian lahan kering (48,94 ha), sawah (6,35 ha), dan permukiman (1,08 ha). Sedangkan kelas tutupan lahan yang mengalami penurunan adalah kelas tutupan lahan semak belukar (80,97 ha), lahan terbuka (40,01 ha), dan perkebunan campuran (18,79 ha). Perubahan tutupan lahan yang terjadi di Kecamatan Sukamakmur Kabupaten Bogor menunjukkan sebesar 73,86% mengalami perubahan ke arah positif dan 26,14% mengalami perubahan ke arah negatif. Perubahan ke arah positif yang lebih besar menunjukkan bahwa cadangan karbon di Kecamatan Sukamakmur semakin meningkat sehingga tanaman mampu menyerap CO2 dari atmosfer lebih banyak dan menyimpannya dalam bentuk biomassa tanaman., Rapid population growth results in the level of people's need for land increasing, causing changes in land cover. This research aims to identify and analyze changes in land cover in Sukamakmur District, Bogor Regency using Sentinel-2 Imagery in 2018–2023. There are 9 land cover classes, namely forest, rice fields, mixed gardens, dry land agriculture, settlements, open land, bushes, rivers, and lakes. The land cover classes that experienced an increase were forest land cover (83.40 ha), dry land agriculture (48.94 ha), rice fields (6.35 ha), and settlements (1.08 ha). Meanwhile, the land cover classes that experienced a decline were the land cover classes of shrubs (80.97 ha), open land (40.01 ha), and mixed plantations (18.79 ha). Land cover changes that occurred in Sukamakmur District, Bogor Regency showed that 73.86% experienced changes in a positive direction and 26.14% experienced changes in a negative direction. Changes in a greater positive direction indicate that carbon reserves in Sukamakmur District have increased so that plants are able to absorb more CO2 from the atmosphere and store it in the form of plant biomass.
Keyword: tutupan lahan, sentinel-2, Sukamakmur

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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