Judul: Analisis Kemitraan Agroforestri antara Kelompok Tani dengan PT BUMN Hijau Lestari I di Kabupaten Bandung Jawa Barat
Abstrak: PT BUMN Hijau Lestari I (PT BHL) is applying the concept of agroforestry partnership with the community to implement the mandate reforestation in watersheds. Partnership in Bandung regency has built over three years. This research aims to identify pattern of agroforestry partnership between KMK and PT BHL, analyze the performances of plants cooperation based aspect of percent growing plants and crops coffee and analyze the level of partnership. This research was done to 20 of 59 KMK who have been partnered since 2010. The result showed that, partnership built is cooperation patterns operational agribusiness (COA) that is both long term. Performance plant cooperation only reached 79% (woody plant), 37% (fruit trees) and 77% (coffee plant). The level of partnership scored 659.3 (Prima Intermediate Partnership) which means this partnership still needs to increase cooperation especially optimization aspect benefits.
Keyword: prima intermediate, performance, partnership, agroforestry

Judul: Pola Kemitraan dalam Pembangunan Hutan Rakyat Oleh PT Insan Prima Berdikari di Kabupaten Pandeglang Banten
Abstrak: Pengelolaan hutan rakyat yang terjadi sampai saat ini sebagian besar masih belum dapat meningkatkan kesejahteraan masyarakat dan petani. Hadirnya kemitraan dalam pengelolaan hutan rakyat dapat mendorong petani berperan lebih aktif dan mengubah pengelolaan hutan rakyat yang dulunya masih tradisional menjadi ke arah komersial. Tujuan pada penelitian ini untuk mengetahui sistem pengelolaan dan menganalisis pola hubungan kemitraan yang diselenggarakan oleh PT Insan Prima Berdikari (PT IPB) dengan para mitranya. Pengambilan data menggunakan metode survey dengan pemilihan responden secara purposive sebanyak 32 orang terdiri dari penggarap lahan, pemilik lahan, dan PT. IPB. Hasil penelitian menunjukan bahwa hutan rakyat dikembangkan dengan pola tanam agroforestri antara pohon sengon dengan tanaman pisang dan talas. Pola hubungan kemitraan antara PT IPB dengan para mitranya merupakan Kerjasama Operasional Agribisnis (KOA) dengan sistem bagi hasil penjualan sengon sebagai berikut: PT IPB mendapat 25%, penggarap lahan 10%, penyedia dana 35%, lembaga desa, dan pendamping desa yang masing-masing pihak tersebut 2,5%., Most of the community forest management that has occurred so far has not been able to improve the welfare of the community and farmers. The presence of partnerships in community forest management can encourage farmers to play a more active role and change community forest management from traditional to commercial ones. The purpose of this study is to determine the management system and analyze the pattern of partnership relationships held by PT Insan Prima Berdikari (PT IPB) with its partners. Data were collected using a survey method with a purposive selection of 32 respondents consisting of land cultivators, land owners, and PT. IPB. The results showed that community forests were developed with an agroforestry cropping pattern between sengon trees and banana and taro plants. The pattern of the partnership relationship between PT IPB and its partners is the Agribusiness Operational Cooperation (KOA) with a profit-sharing system from the sale of sengon as follows: PT IPB gets 25%, land cultivators 10%, fund providers 35%, village institutions, and village assistants each each party is 2.5%.
Keyword: community forest, partnership, profit sharing, agroforestry

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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