Judul: Uji Toksisitas Recombinant Growth Hormone terhadap Artemia salina Leach.
Abstrak: Hormon pertumbuhan rekombinan (rGH) merupakan protein yang diproduksi dengan memanfaatkan bakteri Escherichia coli yang membawa vektor ekspresi gen hormon pertumbuhan dan terbukti mampu meningkatkan laju pertumbuhan ikan maupun udang. Potensi toksisitas rGH ikan kerapu kertang (rElGH) belum diketahui. Penelitian ini bertujuan untuk menentukan tingkat dan golongan toksisitas rGH ikan kerapu kertang terhadap A. salina menggunakan metode brine shrimp lethality test (BSLT). Konsentrasi rElGH yang digunakan, yaitu: A. 1,5; B. 15; C. 150; dan D. 1,500 mg L-1 dengan lima kali ulangan. Sebagai kontrol, digunakan dua perlakuan, yakni perlakuan K1 hanya menggunakan 20 mL air laut, dan perlakuan K2 dengan pemberian 3 mL PBS (pelarut rElGH) dan 17 mL air laut. Kepadatan A. salina yang digunakan yakni 100 ekor dalam 20 mL larutan yang berisi 3 mL rElGH dan 17 mL air laut. Pengamatan terhadap jumlah naupli yang mati dilakukan setiap 6 jam sekali selama 24 jam pengamatan. Persentase kematian pada perlakuan A, B, C, dan D berturut-turut ialah 4,0%; 11,2%; 26,6%; dan 100%. Berdasarkan hasil analisis probit Minitab 16, nilai LC50 adalah 222,05 mg L-1, sehingga rElGH digolongkan dalam senyawa dengan tingkat toksisitas rendah.
Keyword: A. salina, BSLT, rElGH, toksisitas

Judul: Respons benih ikan sidat terhadap hormon pertumbuhan rekombinan ikan kerapu kertang melalui perendaman dan oral
Abstrak: This study was aimed to determine an appropriate administration method and observe the response of eel fingerling on recombinant giant grouper growth hormone (rElGH). First study aimed to determine the immersion dose of rElGH to increase growth of eel juvenile (glass eel). After shock salinity treatment (NaCl 3% for 2 min), glass eel were immersed in water containing 0.9% NaCl, 0.01% bovine serum albumin, and different of dose of rElGH (0, 0.12, 1.2, 12 and 120 mg/L). The results showed that higher in growth body weight was obtained in treatment 12 mg/L (101.2±0.8), by increment of about 37.4% higher compared to that of control (72.93±1.8). Second study was aimed to determine the dose of rElGH mixed in artificial diet to increase growth of eel elver. HP55-coated rElGH was mixed to diet at different doses (0, 0.3, 3, and 30 mg/kg). Elver was fed on diet containing rElGH twice daily, 6% fish biomass. The result showed that higher in growth body weight was obtained in treatment 30 mg/kg (60.18±1.38), by increment of about 65.7% compared to control (36.32±0.97). Third study was aimed to compared of three administration method, namely immersion, oral, and combination of immersion and oral. The dosage of rElGH by immersion (12 mg/L) and oral administration (30 mg/kg feed) was obtained in previous study. Fish were maintained in 23-L glass aquaria for 120 days, at density of 45 fish per aquarium. The results showed that higher (P<0.05) biomass of harvest (73.68±2.07 g) was obtained in immersion and oral combination treatment, by increment of about 102.9% compared to control (36.32±0.97 g). Administration of rElGH also improved protein and lipid retentions by 142.5%, and 720% compared to that of control, respectively (P<0.05). In addition, rElGH treatment increased appetite, while artificial feed conversion ratio (4.75) was lower (P<0.05) by 55.15% compared to that of control (7.37). Combination of rElGH immersion and oral administrations also increased hepatosomatic index, reduced ammonia excretion and increased insuline-like growth factor-1/IGF-1 gene expression. Thus, rElGH administration via immersion and oral was a proper method to improve performance of eel fingerling, and application of rElGH can be useful to increase aquaculture production.
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Judul: Simulasi Protokol Autentikasi Pelanggan pada Voice Privacy
Abstrak: Existing design of the telephone public network is susceptible to eavesdropping on its voice stream. Consequently, any wire taper can listen to supposedly private conversation. In order to prevent such eavesdropping, we propose a protocols and its implementation for end-to-end voice privacy authenticate as a service available for interested subscribers. Our protocols consist of customer enrollment system, customer authentication system and simulation voice privacy. Protocols that we made are adaptation from Sharif and Wijekesera journal with the title Providing Voice Privacy as a Service over the Public Telephone Network. The result of the process shows how the customer enrolls and how the system process the password, how the system authenticate customer simulate voice privacy.
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