Judul: Karakterisasi Gen Hormon Pertumbuhan (Chicken Growth Hormone) pada Ayam Lokal Indonesia
Abstrak: Ayam lokal merupakan salah satu sumber daya genetik yang berpotensi untuk dikembangkan. Karakterisasi genetik yang berkaitan dengan sifat produksi dapat diketahui berdasarkan gen, salah satunya adalah gen cGH. Penelitian ini bertujuan untuk mengidentifikasi karakteristik genetik dan mendeteksi situs mutasi pada gen cGH. Sampel yang digunakan adalah ayam kampung, walik, arab dan leher gundul. DNA hasil ekstraksi kemudian dilakukan proses amplifikasi fragmen gen cGH dan sekuensing. Sebanyak 19 sampel berhasil teramplifikasi. Amplifikasi gen cGH menghasilkan produk PCR sepanjang 528 bp. Hasil sekuensing DNA dianalisis menggunakan program MEGA 5. Hasil analisis sekuens fragmen gen cGH menunjukan nilai kesamaan sebesar 99% dengan data sekuens gen cGH pada GenBank. Mutasi pada fragmen gen cGH ditemukan sebanyak 10 situs dengan tipe mutasi transisi dan transversi. Penelitian lanjutan diperlukan untuk mengidentifikasi karakteristik genetik gen cGH pada G. g. spadiceus, G. g. bankiva, G. g. murghi dan G. g. jabouillei.
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Judul: Keragaman Gen Hormon Pertumbuhan pada Ayam Lokal Indonesia dan Persilangannya
Abstrak: Indonesia local chickens have genetic potency with high ecomonic value such as meat and egg production, adapted in tropic and strong immunity. The chicken growth hormone (cGH) gene has a crucial role in controlling growth and metabolism, leading to potential correlations between cGH gene polymorphism and economic traits. This study aimed to identify the cGH gene polymorphism and find out the variation of gene at several local chickens, the crossbred, commercial meat type and layer. Polymorphism in intron 4 of cGH gene was studied in 229 chicken blood samples consisted of kampung, pelung, sentul, commercial meat type, layer, merawang, commercial meat type x kampung, kampung x commercial meat type, sentul x kampung and pelung x sentul chickens. A specific primer set was used to amplify a fragment of growth hormone locus using PCR. PCR products were digested with MspI restriction endonucleases. The amplified fragment digested with MspI enzyme revealed 2 alleles A and B with the frequency of 0.88-0.96 and 0.04-0.12, except in layer chickens only had 1 allele A with the frequency of 1.00. The results showed that cGH gene in MspI locus at kampung, pelung, sentul, commercial meat type, merawang, commercial meat type x kampung, kampung x commercial meat type, sentul x kampung and pelung x sentul chickens were polymorphics, except the layer chickens was monomorphics. The population of Indonesia local chicken and the crossbred that was studied in this research had low heterozygosity value but it was still in the Hardy-Weinberg equilibrium.
Keyword: polymorphism, PCR-RFLP, local chicken, MspI, cGH

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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