Judul: Coastline Change Using Landsat Satellite Imagery on the North Coast of Java, Banten
Abstrak: Perubahan garis pantai yang disebabkan abrasi dan akresi akan menunjukkan kecenderungan perubahan garis pantai yang terkikis atau bertambah. Deteksi perubahan garis pantai menggunakan teknologi penginderaan jauh merupakan salah satu metode observasi yang dapat dilakukan secara multi temporal. Tujuan penelitian untuk mengetahui perubahan garis pantai dengan menggunakan citra satelit di Pantai Utara Jawa, Banten dengan metode Digital Shoreline Analysis System (DSAS). Proses analisis data meliputi empat tahapan, yaitu pengolahan citra awal, ekstraksi garis pantai, perhitungan perubahan garis pantai, dan pengolahan data pendukung. Kecepatan angin dominan berasal dari arah barat yang berkisar antara 3,60-5,70 m/s. Pergerakan arus di daerah kajian menunjukkan pergerakan arus bergerak dari timur menuju barat. Nilai tinggi gelombang mencapai 0,21 m dengan periode gelombang 0,01-3,09 m/s. Hasil perhitungan nilai bilangan formzhal menghasilkan nilai tipe pasang surut sebesar 4,05 yang termasuk ke dalam tipe pasang surut tunggal. Abrasi terbesar terjadi di Kecamatan Mauk dengan kemunduran garis pantai sebesar 928,55 m, sedangkan akresi terbesar terjadi di Kecamatan Kemiri dengan penambahan garis pantai sebesar 380,99 m. Kata Kunci : Abrasi, Akresi, DSAS, Ekstraksi, Penginderaan Jauh
Keyword: Abrasion, Accretion, DSAS, Extraction, Remote Sensing

Judul: Deteksi Perubahan Garis Pantai di Kabupaten Indramayu, Jawa Barat
Abstrak: Pesisir Kabupaten Indramayu memiliki kerentanan pesisir yang tinggi akibat interaksi darat dan laut sehingga mudah mengalami perubahan secara fisik seperti perubahan garis pantai. Deteksi perubahan garis pantai menggunakan teknologi penginderaan jauh merupakan salah satu metode observasi yang dapat dilakukan secara multi temporal. Penelitian ini bertujuan untuk memetakan dan mengetahui perubahan garis pantai di Kabupaten Indramayu, Jawa Barat menggunakan citra Sentinel-2A pada tahun 2016 dan 2022 dengan metode Digital Shoreline Analysis System (DSAS). Hasil dari metode DSAS didapatkan nilai perubahan garis pantai yang memiliki nilai positif dan negatif. Nilai positif berarti pantai mengalami akresi sedangkan nilai negatif pantai mengalami abrasi. Laju abrasi tertinggi terjadi di Desa Paben llir, sementara itu akresi tertinggi terjadi di Desa Krangkeng. Faktor–faktor terjadinya abrasi ialah gelombang, arus, pembukaan lahan seperti tambak sehingga terjadi kemunduran garis pantai. Faktor–faktor terjadinya akresi adalah proses sedimentasi oleh aliran sungai. Kata Kunci: Abrasi, Akresi, DSAS, Garis Pantai., The coast of Indramayu Regency had high coastal vulnerability due to the interaction of land and sea so it easily experiences physical changes such as changes in coastlines. Detection of coastline changes using remote sensing technology is an observation method that can be carried out multi-temporally. This research aims to map and determine coastline changes in Indramayu Regency, West Java using Sentinel-2A imagery in 2016 and 2022 using the Digital Shoreline Analysis System (DSAS) method. The results of the DSAS method show coastline change values with positive and negative values. A positive value means the beach is experiencing accretion while a negative value is that the beach is experiencing abrasion. The highest abrasion rate occurred in Paben Illir Village, meanwhile, the highest accretion occurred in Krangkeng Village. The factors for abrasion were waves, currents, and the conversion of mangroves into ponds resulting in a decline in the coastline. The factors that cause accretion were sedimentation processed by river flows. Keywords: Abrasion, Accretion, DSAS, Shoreline
Keyword: Abrasi, Akresi, DSAS, Garis Pantai

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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