Judul: An Analysis of Attitude Model: Relationships among Perception, Affective, Preference, and Intention to Buy Batik Clothing.
Abstrak: Tujuan dari penelitian ini adalah untuk menganalisis model sikap, terkait dengan hubungan antara variabel persepsi, afektif, preferensi, dan minat beli pakaian batik. Metode survey dari disain cross sectional study ini dilakukan dengan menggunakan 350 mahasiswa Institut Pertanian Bogor sebagai responden. Uji korelasi Pearson digunakan untuk menganalisis hubungan antara variabel persepsi, afektif, preferensi, dan minat beli pakaian batik. Analisis Conjoint digunakan untuk menganalisis preferensi contoh terhadap pakaian batik. Hasil uji regresi menunjukkan bahwa persepsi tidak berpengaruh secara signifikan. Terkait dengan hal yang berpengaruh terhadap minat beli pakaian batik, variabel afektif dan preferensi berpengaruh secara signifikan terhadap minat beli pakaian batik.
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Judul: self concept, lifestyle, and purchasing behavior to batik cloth forward undergraduate students.
Abstrak: Tujuan dari penelitian ini adalah menganalisis kepribadian, konsep diri, gaya hidup dan perilaku pembelian pakaian batik pada mahasiswa. Metode penelitian ini menggunakan desain cross sectional study dengan jumlah contoh 350 orang mahasiswa Institut Pertanian Bogor. Penelitian ini dianalisis menggunakan analisis deskriptif, Crosstab, Chi-Square, dan K Means Cluster. Berdasarkan uji K Mean Cluster, kepribadian mahasiswa IPB adalah extraversion, yaitu mudah dekat dengan orang lain; konsep diri ideal self, yaitu memandang dirinya ideal dimata orang lain; dan gaya hidup individualis, yaitu gaya hidup bersenang-senang, berprestasi, dan mengatur diri sendiri. Perilaku pembelian pada jenis batik adalah batik tulis dengan memperhatikan pada atribut produk dalam pembelian, sedangkan asal batik cenderung membeli batik lokal dengan memperhatikan atribut produk. Hasil Crosstab menunjukkan, contoh yang memiliki kepribadian (extraversion, consciouness, dan sophistication), konsep diri (actual self dan ideal self), dan gaya hidup individualis cenderung membeli batik tulis dibandingkan batik cap dan print. Semua dimensi kepribadian, tipe konsep diri, dan gaya hidup contoh lebih cenderung membeli pakaian batik lokal dibandingkan impor. Hasil Chi-Square menunjukkan hubungan antara jenis kelamin dengan tingkat kesukaan contoh pada jenis batik (batik tulis, cap, dan print), uang saku berhubungan dengan pattern batik (pakaian jadi dan kain) dan asal batik (batik lokal dan batik impor). Selain itu kepribadian juga berhubungan dengan pattern batik.
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Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable