Judul: Kajian Kemitraan pengelolaan air Taman Nasional Bromo Tengger Semeru di Kecamatan Senduro, Kabupaten Lumajang
Abstrak: Bromo Tengger Semeru National Park (BTSNP) is one of protected area that is managed by involving local people. The collaborative management in this national park is carried out in term of partnership water management by signing agreement of collaboration between national park and some partners. The aims of this study are to identify the partnership water management at BTSNP and finally give recommendation concerning collaboration of water management based on analysis result of impact and partnership problem and evaluation of partnership system. This study hopefully can give information about partnership water management at BTSNP and be useful as consideration to manager and partners for the future partnership.
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Judul: Studi Pemanfaatan Kawasan Laut Pasir Di Taman Nasional Bromo Tengger Semeru
Abstrak: Taman Nasional Bromo Tengger Semeru merupakan satu-satunya kawasan konservasi yang mempunyai keunikan berupa laut pasir. Metode yang digunakan untuk melihat persepsi pengunjung dan masyarakat menggunakan kuesioner dalam bentuk close ended dan menggunakan teknik skala likert . Analisis data dilakukan secara deskriptif dan menggunakan uji statistik chi square. Persepsi dari responden mendapatkan skor 5 (agak setuju) untuk jenis kegiatan yang dilakukan dan kondisi kawasan. Responden memberikan skor 3 (kurang setuju) untuk sumberdaya alam yang dimanfaatkan. Uji chi square untuk aspek persepsi menunjukkan bahwa nilai X2 hitung < X2tabel sebesar 0,95 < 12,59 hal ini menyatakan bahwa jawaban dari responden tidak memiliki perbedaan/ hubungan terhadap pertanyaan yang diberikan. Responden memberikan skor 5 (agak setuju) terhadap dampak ekonomi dan dampak ekologi yang ditimbulkan dari kegiatan pemanfaatan. Uji chi square untuk aspek dampak menunjukkan bahwa nilai X2 hitung > X2tabel sebesar 13,83 > 7,81 hal ini menyatakan bahwa jawaban dari responden memiliki perbedaan/ hubungan terhadap pertanyaan yang diberikan.
Keyword: dampak kegiatan pemanfaatan, laut pasir, pemanfaatan kawasan

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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