Judul: Relationship of Spatial between Topography and  Soil Characteristics of Classification Differentiae in Quaternary Volcanic Hills in  Subang, West Java
Abstrak: Penarikan batas satuan peta tanah (SPT) untuk daerah dengan keragaman topografi yang tinggi dapat didasarkan pada perbedaan topografinya. Namun banyak kasus menunjukkan fakta bahwa di dalam satuan topografi yang sama masih dijumpai keragaman karakteristik tanah yang tinggi. Penelitian ini dirancang untuk mengamati morfologi tanah dengan intensitas tinggi pada area sempit yang memiliki faktor pembentuk tanah homogen kecuali topografi, di perbukitan volkan kuarter di Subang, Jawa Barat. Penelitian ini dimaksudkan untuk mengungkapkan apakah keragaman spasial topografi, khususnya di daerah dengan tipologi seperti area studi, sejalan dengan keragaman karakteristik tanahnya. Hasil penelitian diharapkan dapat dijadikan dasar dalam menentukan strategi pengamatan dan penarikan batas untuk pemetaan tanah khususnya tingkat detil. Penelitian dilaksanakan pada area seluas 10 ha yang merupakan bagian dari PT. Perkebunan Buah Subang dengan melakukan 50 pemboran (boring) secara menyebar menurut segmen lereng dan pengamatan 6 (enam) profil pewakil. Didasarkan pada karakteristik morfologinya ke-6 (enam) profil pewakil telah diklasifikasikan menurut dua sistem klasifikasi. Hasil klasifikasi hingga Subgroup menurut Taksonomi Tanah menunjukkan bahwa ke-6 (enam) profil pewakil merupakan Typic Dystrudepts, yang berarti bahwa seluruh area studi hanya terdiri dari Typic Dystrudepts. Sementara itu hasil klasifikasi hingga kategori Macam Tanah menurut Sistem Klasifikasi Nasional menunjukkan area studi didominasi Latosol Kromik dan sebagian kecil tanahnya termasuk Latosol Haplik. Penelusuran lebih seksama menunjukkan masih ada keragaman morfologi di antara ke-6 (enam) profil yang dideskripsi, terlepas dari hasil klasifikasi yang homogen. Keragaman morfologi yang tergolong menonjol adalah warna tanah, yaitu 3 (tiga) profil memiliki hue 7.5YR, dan 2 (dua) profil memiliki hue 5YR, dan 1 (satu) profil memiliki hue 10YR. Keragaman lainnya antara lain ketebalan horizon genetik dan diagnostik. Namun demikian, hasil penelusuran hubungan antara posisi profil pewakil pada segmen lereng dengan keragaman morfologi tersebut menunjukkan hubungan yang tidak konsisten, yaitu hue dominan 7.5YR atau 5YR atau 10YR dan atau ketebalan horizon genetik dan diagnostik tertentu dari tanah di daerah studi tidak bersifat unik untuk profil pada segmen lereng tertentu., The delination of soil map unit (SMU) boundaries for areas with high topographical variability can be based on the topographic boundaries. However, many cases show the fact that in the same topographic unit there is still a high variability of soil characteristics. This study was designed to intensively observe soil morphology in a small area that has homogeneous soil forming factors except topography, in the quarternary volcanic hills in Subang, West Java. This study is intended to reveal whether the spatial variability of topography, especially in areas with a typology such as the study area, is in line with the variability of soil characteristics. The results of the research are expected to be used as a basis for determining the strategy of soil observation and SMU delineation, especially for the detailed mapping. The research was carried out in an area of 10 ha which is part of PT. Buah Subang by carrying out 50 augers spread according to the slope segment and observing six representative profiles. Based on their morphological characteristics, the six representative profiles have been classified according to two classification systems. The results of the classification to Subgroups according to Soil Taxonomy show that the six
Keyword: morfologi tanah pembeda, pemetaan tanah detil, satuan peta tanah

Judul: Soil Heterogeneity Along Short Toposequence of Hilly Slopes on Quaternary Volcanic Geological Formations in Subang, West Java
Abstrak: Topografi merupakan salah satu faktor yang menentukan keragaman tanah di suatu wilayah. Pemilahan spasial kondisi topografi yang meliputi aspek-aspek lereng sangat penting diperhatikan dalam penarikan Satuan Peta Tanah (SPT), khususnya pada pemetaan tanah detil. Penelitian ini bertujuan mengetahui keragaman karakteristik tanah sepanjang toposekuen pendek yang terdiri dari segmen-segmen lereng berdasarkan ragam aspek lereng untuk melihat korelasi antar aspek-aspek lereng dengan karakteristik, klasifikasi, dan tingkat perkembangan dan pelapukan tanah. Penelitian dilaksanakan di PT. Perkebunan Buah Subang pada suatu toposekuen dengan panjang sekitar 1 km melintang dari puncak bukit hingga lembah di dekatnya. Toposekuen tersebut berada pada suatu formasi geologi dan zona iklim yang sama. Sepanjang toposekuen yang diteliti terdapat empat segmen lereng ditemukan karakteristik tanah beragam dan dijumpai korelasi antara beberapa ciri-ciri morfologi, tekstur tanah, dan kejenuhan basa dengan segmen lereng. Tingkat perbedaan beberapa keragaman karakteristik tanah yang dijumpai tidak seluruhnya menyebabkan perbedaan klasifikasi tanahnya yang termasuk ke dalam dua Subgrup yaitu Typic Dystrudepts dan Oxic Dystrudepts. Tingkat perkembangan dan pelapukan tanah pada toposekuen yang diteliti sulit diukur oleh parameter yang digunakan dalam penelitian ini. Terlepas dari lemahnya korelasi segmen lereng dengan karakteristik dan klasifikasi tanah yang dijumpai dalam penelitian ini, berdasarkan konsep dan teknik penarikan SPT khususnya untuk peta tanah detil, segmen lereng yang didasarkan pada aspek-aspek lereng tetap penting dijadikan dasar penarikan batas SPT, namun tidak selalu menjadi petunjuk pasti terkait sebaran individu tanah pada setiap kategori klasifikasinya untuk inter dan intra SPT., Topography is one of the major factors that determine the heterogeneity of soil in an area. Topographical separation that considers slope aspects is essential in the delineation of Soil Map Units (SMU), especially for the detail soil mapping. This study aims to determine the diversity of soil characteristics along a short toposequence consisting of slope segments based on various slope aspects to see the correlation between slope aspects with the soil characteristics, classification, and level of soil development and weathering. The research was conducted at PT. Perkebunan Buah Subang with the toposequence length of approximately 1 km across from the top of the hill to the nearby valley. The toposequence is located in the same geological formation and climate zone. Throughout the selected toposequence, four slope segments were found with variations in some soil characteristics of each and the correlations between the slope segments and the respected soil characteristics were indicated for few morphological feature, soil texture, and base saturation. Based on the analysis, the differences in some of the soil characteristics did not entirely cause distinction in soil classification which were classified into two subgroups, namely Typic Dystrudepts and Oxic Dystrudepts. The level of soil development and weathering in the toposequence was difficult to measure by the parameters used in this study. Regardless of the weak correlation between the slope segments with soil characteristics and classification, based on the concept and technique of delineating the boundaries of SMU, especially for detailed soil maps, slope segmentation based on slope aspects is still critical to be considered. However, it is not always a definite guide regarding the distribution of individual soil in each classification category for inter and intra
Keyword: faktor pembentuk tanah, keragaman tanah, korelasi, satuan peta tanah, segmen lereng

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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