Judul: Pengaruh Panjang dan Batang Penyusun Kolom terhadap Faktor Tekuk Bambu Andong
Abstrak: Pemanfaatan kayu sebagai bahan konstruksi masih menjadi pilihan utama, sehingga penggunaan bahan alam dengan sifat yang sebanding perlu dilakukan. Bambu andong memiliki sifat yang sebanding dengan kayu sebagai bahan konstruksi. Penelitian ini bertujuan untuk memberikan informasi terkait kapasitas tekuk dan nilai faktor tekuk bambu andong pada kolom tunggal dan multikolom untuk komponen struktur bangunan. Tekuk merupakan peristiwa ketika struktur kolom tidak mampu lagi mempertahankan bentuk aslinya. Nilai kapasitas tekuk tertinggi dimiliki oleh kolom rangkap 4 berukuran panjang 1 meter sebesar 170 kN, sedangkan nilai kapasitas tekuk terendah terdapat pada kolom tunggal dengan panjang 6 meter sebesar 4 kN. Kolom struktur dikatakan aman ketika nilai faktor tekuk empiris yang dianalisis lebih besar dibandingkan faktor tekuk teoritis. Nilai faktor tekuk empiris karakteristik (Rk) dan batas bawah 5% (R0.05) menghasilkan nilai yang lebih tinggi dibandingkan faktor tekuk teoritis pada semua komponennya sehingga aman digunakan dalam analisis struktur., Bamboo has properties comparable to wood as a construction material. Andong bamboo is a natural solid material to be used in the construction world. This study aims to provide information regarding the buckling capacity and buckling factor values of andong bamboo in single and multi-column columns for building structural components. Buckling is an event when the column structure is no longer able to maintain its original shape. The highest buckling capacity value is owned by a double-four column measuring 1 meter in length of 170 kN. In contrast, the lowest buckling capacity value is found in a single column with a length of 6 meters at 4 kN. The structural column is said to be safe when the empirical buckling factor analyzed is greater than the theoretical buckling factor. The empirical buckling factor characteristic capacity (Rk) and the lower limit of 5% (R0.05) produces a higher value than the theoretical
Keyword: Andong bamboo, buckling factor, buckling capacity, column, multicolumn

Judul: Analisis Tekuk Kolom Bambu Hitam Sebagai Komponen Struktur Bangunan
Abstrak: Bambu merupakan salah satu alternatif biomaterial terbarukan yang dapat dikembangkan sebagai bahan konstruksi dan bersifat komplementer terhadap kayu. Bambu hitam (Gigantochloa atroviolaceae) merupakan tanaman yang tumbuh lurus dengan batang hitam kuat yang dapat dimanfaatkan sebagai bahan konstruksi. Penelitian ini bertujuan memberikan informasi terkait kapasitas tekuk dan nilai faktor tekuk bambu hitam pada kolom tunggal dan multikolom sebagai komponen struktur bangunan. Contoh uji yang digunakan merupakan bambu hitam dengan ukuran 4 dan 6 m serta ditambahkan data hasil pengujian sebelumnya dengan berukuran 1, 2, dan 3 m untuk analisis faktor tekuk. Pengujian tekuk dilakukan menggunakan mesin uji tekuk kolom bambu ukuran penuh kapasitas 100 ton. Nilai kapasitas tekuk tertinggi dimiliki oleh kolom rangkap empat berukuran panjang 1 m yaitu 207 kN, sedangkan kolom tunggal dengan panjang 6 m memiliki kapasitas tekuk terendah yaitu 3 kN. Hasil analisis faktor tekuk menunjukkan bahwa kapasitas tekuk karakteristik dan batas bawah 5% yang telah dikoreksi faktor tekuk aman digunakan dalam analisis struktur kolom bambu hitam., Bamboo is an alternative renewable biomaterials developed as a construction material and complementary to wood. Hitam Bamboo (Gigantochloa atroviolaceae) is a plant that can grow straight with intense black culm that can be used as construction materials. This study aims to Hitam Bamboo in single and multi-culm columns as components of building structures. The test samples used were Hitam bamboo with sizes of 4 and 6 m and added data from the results of previous tests with sizes of 1, 2, and 3 m for buckling factor analysis. Buckling testing is carried out using a full-size bamboo column buckling testing machine with a capacity of 100 tons. The highest buckling capacity value is owned by a four-culm column measuring 1 m long, 207 kN. In contrast, a single column with 6 m length has the lowest buckling capacity of 3 kN. The results of the buckling factor analysis showed that the characteristic buckling capacity and the lower limit of 5% corrected by buckling factor are safe for analyzing bambu hitam column structures.
Keyword: buckling capacity, buckling factor, column, hitam bamboo, multicolumn

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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