Judul: Perangkat Mikrofluida berbasis Penyeka Kapas untuk Deteksi Kebocoran Membran Amnion Sapi secara Semi Kuantitatif.
Abstrak: Deteksi kebocoran kantung amnion dini pada sapi sangat dibutuhkan untuk menghindari kerugian ekonomi pada peternak karena matinya anak sapi yang lahir secara prematur. Metode yang dikembangkan pada penelitian ini menggunakan reagen Bromocresol Green (BCG) untuk mendeteksi kenaikan konsentrasi Bovine Serum Albumin (BSA) pada urin sapi. Konsep alat dalam penelitian ini mengacu pada strip test yang telah digunakan di dunia kedokteran manusia, yaitu adanya rangkaian pendeteksi serta rangkaian yang menjadi parameter positif-negatif. Perangkat berkonsep mikrofluida yang dikembangkan memiliki dua bagian utama yaitu penyeka kapas sampling dan penyeka kapas kalibrator dari penyeka kapas yang tersusun oleh tiga benang katun. Penyeka kapas mampu menyerap contoh urin sebanyak 90-120 μL dalam 4 detik. Albumin pada urin sapi dapat dideteksi menggunakan reagen yang dikandungkan pada alat. Limit of Detection (LoD) pada perangkat mikrofluida memiliki nilai 0,04 g/dL dan Limit of Quantification (LoQ) 0,1 g/dL. Perubahan warna hasil reaksi antara BCG dan BSA pada benang dapat dilihat langsung oleh mata tanpa bantuan alat dalam waktu 120 detik pasca pencelupan.
Keyword: cotton swab, cow, microfluid, premature rupture of membranes, semiquantitative

Judul: Dip Spray Stick Microfluidic Device for Early Pregnancy Detection in Sheeps
Abstrak: Deteksi kebuntingan dini sangat diperlukan untuk meningkatkan produksi yang efisien dan hemat biaya pada peternakan domba di Indonesia. Deteksi kebuntingan dini menggunakan barium klorida (BaCl2) memerlukan sampel dan reagen dalam volume besar dan peralatan khusus. Penelitian ini bertujuan untuk mengembangkan alat deteksi kebuntingan dini pada domba yang praktis dan ekonomis dengan melakukan penyederhanaan prinsip uji BaCl2. Perangkat mikrofluida yang dikembangkan menggunakan kain katun sebagai bahan penyerap, filter, dan tempat terjadinya reaksi. Kain katun dengan pencucian satu kali menghantarkan cairan lebih optimal. Kain katun mampu menyerap cairan sebanyak 53µL selama 10,6 detik. Kristal BaCl2 terlihat jelas pada kain katun yang mengandung urin setelah dikeringkan selama nol sampai 15 menit. Kristal BaCl2 hasil reaksi pada kain katun dapat dilihat secara langsung tanpa bantuan alat pada konsentrasi 25-50% pada menit ke sepuluh. Nilai LoD dan LoQ perangkat mikrofluida secara berurutan adalah 0,010 g/mL dan 0,031 g/mL., Early pregnancy detections are strongly needed to increase production efficiency and cost-effective production on sheep farms in Indonesia. Early pregnancy detection using barium chloride (BaCl2) requires a large volume of samples and reagent and specialized equipment. This study was aimed to develop a practical diagnostic device to detect early pregnancy in sheep by simplifying the principles of the BaCl2 test. The microfluidic device was developed using a cotton cloth as an absorbent, filter, and reaction site. The cotton cloth with single washing had proven to be more optimal in delivering fluids. The results showed that evaporation of urine affected the formation of the BaCl2 crystals. BaCl2 crystals clearly inspected at the cotton cloth that has been contained by urine after zero up to 15 minutes evaporation. The result of the BaCl2 crystallization at the cotton cloth clearly inspected by eyes without supporting tool at the concentration of 25% up to 50%. The Limit of Detection and Limit of Quantification of the microfluidic device were 0.010 g/mL and 0.031 g/mL.
Keyword: barium chloride, cotton cloth, early pregnancy detection, microfluidic, sheep

Judul: Thermal Characteristics and Dynamics of Tropical Cyclone over Western North Pacific Ocean: A Case Study of Typhoon Songda (Chedeng) 2011
Abstrak: The frequent occasion of Tropical Cyclone (TC) over Western North Pacific (WNP) Ocean lies from 10˚N to 26˚N and 121˚E to 170˚E. Sources of TC development come from warm ocean temperature where latent heat is released during condensation processes and subsequently concentrated in the boundary layer. Relative humidity at 850-700 hPa is responsible to produce the most intense storm. Meanwhile, weak vertical wind shear between 850-200 hPa is one of the dynamical factors that lead to the development of TC. Pressure level of 850-200 hPa is chosen to minimize the thermodynamic effect in the lower troposphere. Typhoon Songda (Chedeng in the Philippines) or TSC lasted from May 18th to 30th, 2011 and strengthened into powerful Super Typhoon stage between May 24th and 27th. Long curving track and the exhibition of all sequences of TC life cycle, TSC is then considered as an ideal case of TC. The objective of the study with case of TSC is to investigate the role of thermal characteristics and dynamics on TC intensity. The research is carried out by using Advanced Research of Weather Research and Forecasting model (WRF ARW model) version 3.3. Two domains are employed in the model. The first domain situate from 02˚N to 40˚N and 118˚E to 149˚E and cover spatial resolution () of 33.1 km. The second domain situate from 09˚N to 27˚N and 119˚E to 132˚E with of 11 km. Timestep of the model () is 120 seconds. The model is supported by the following physical parameterization, i.e., Yonsei University Scheme (YSU) in the boundary layer, Kain-Fritsch scheme (KF) in the cumulus, and WRF Single-Moment 3-class scheme in the microphysics. Initial meteorological condition prior to the formation of the cyclone is generated using WRF ARW standard-release model. Sensitivity of the model output is obtained from a selected single-parameter change scenarios, namely, 10 % to 20 % decrease and increase of wind shear initial data at 850-200 hPa, and by 10 % to 20 % decrease of relative humidity initial data at 850-700 hPa. The result showed that the negative correlations between wind shear and intensity are found in wind shear scenarios. Wind shears of 10 ms-1 which lead to strengthening of the wind intensity in the early period of Typhoon development are found in wind shear scenarios. The effect of wind shear changes on wind intensity is statistically significant in the 20 % wind shear scenarios. During mature stage, weak wind intensity occurred when wind shear increase as given in the WRF standard-release and the 20 % decreasing scenario. Different situation is found in the 20 % increasing scenario where wind shear tend to weak and lead to strong wind intensity at this stage. Meanwhile, the reduction of moisture supply at 850-700 hPa resulting from 20 % decreasing relative humidity play a major role in the weakening of wind intensity and reduces mechanical energy of the cycle process as much as 300 J kg-1.
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