Judul: Hubungan antara Pengetahuan, Sikap, dan Praktik Gizi dengan Frekuensi Jajan Anak Sekolah Dasar di Kota Tangerang Selatan
Abstrak: Makanan jajanan merupakan bagian yang tidak dapat terpisahkan dengan anak sekolah. Penelitian ini bertujuan mengetahui hubungan antara pengetahuan, sikap dan praktik gizi dengan frekuensi jajan anak sekolah dasar di Kota Tangerang Selatan. Desain penelitian berupa cross-sectional study dengan 105 subjek penelitian. Data meliputi karateristik contoh, karakteristik keluarga, pengetahuan, sikap dan praktik gizi, serta frekuensi jajan. Sebagian besar pengetahuan dan sikap gizi contoh tergolong sedang (52.4%) dan baik (59%), hanya saja praktik gizi sebagian besar (41%) tergolong kurang. Sebagian besar contoh (81%) memiliki frekuensi jajan ≥4 kali/hari. Hasil uji korelasi chi-square menunjukkan bahwa tidak terdapat hubungan yang signifikan antara karakteristik contoh (jenis kelamin, usia dan besar uang saku), pekerjaan orang tua dan pendapatan orang tua dengan frekuensi jajan (p>0.05). Sedangkan, adanya hubungan yang sigmifikan antara pendidikan ayah (p=0.000) dan pendidikan ibu (p=0.004) dengan frekuensi jajan. Hasil uji korelasi chi-square menunjukkan bahwa tidak terdapat hubungan yang signifikan antara pengetahuan, sikap dan praktik gizi (>0.05) dengan frekuensi jajan.
Keyword: anak sekolah, frekuensi jajan, pengetahuan gizi, praktik gizi, sikap gizi

Judul: Hubungan Pengetahuan serta Sikap Gizi dan Keamanan Pangan dengan Kebiasaan Jajan dan Status Gizi Siswa
Abstrak: Anak usia sekolah dasar cenderung memiliki kebiasaan jajan dan belum memiliki pengetahuan serta sikap yang baik terkait gizi keamanan pangan. Tujuan penelitian adalah menganalisis hubungan pengetahuan serta sikap gizi dan keamanan pangan dengan kebiasaan jajan dan status gizi siswa. Penelitian menggunakan desain cross sectional serta melibatkan 71 subjek kelas IV dan V SDN Ciawi 02. Penelitian dilakukan bulan Mei 2024 melalui wawancara, pengisian kuesioner, serta pengukuran antropometri. Instrumen pengetahuan dan sikap diukur menggunakan kuesioner terstruktur, asupan dengan Food Recall serta Food Frequency Questionnaire, dan status gizi dengan berat dan tinggi badan. Mayoritas subjek memiliki pengetahuan sedang (63,4%) dan sikap netral (54,9%), tergolong sering jajan (76,1%), kontribusi zat gizi jajanannya berlebih (>20% ), dan status gizinya tergolong normal (69%). Hasil penelitian menunjukkan tidak terdapat hubungan signifikan antara pengetahuan serta sikap gizi dan keamanan pangan dengan kebiasaan jajan dan status gizi subjek (p>0,05). Faktor yang berpengaruh terhadap asupan energi jajanan adalah jenis kelamin, usia, pendidikan ayah, pekerjaan ayah, pendidikan ibu, pendapatan ibu, dan sikap subjek (p<0,05). Faktor yang berpengaruh terhadap asupan protein jajanan adalah jenis kelamin, usia, pekerjaan ayah, dan pendidikan ibu (p<0,05). Faktor yang berpengaruh terhadap asupan lemak jajanan adalah jenis kelamin, usia, pekerjaan ayah, pendidikan ibu, dan sikap subjek (p<0,05)., Elementary school-age children have snacking habits and not yet having good knowledge and attitudes related to food safety nutrition. The purpose was to analyze relationship between knowledge and attitudes towards nutrition and food safety with snacking habits and nutritional status. The study used a cross-sectional design and involved 71 subjects of class IV and V SDN Ciawi 02. The study was conducted in May 2024 through interviews, filling out questionnaires, and anthropometric measurements. Knowledge and attitude instrument measured using structured questionnaires, intake with Food Recall and Food Frequency Questionnaire, and nutritional status with weight and height. The majority of the subjects had moderate knowledge (63.4%), neutral attitudes (54.9%), were classified as frequent snacks (76.1%), their snack nutrient contribution was excessive (>20%), and their nutritional status classified as normal (69%). There was no significant relationship between knowledge and attitudes with snacking habits and nutritional status of subjects (p>0.05). Factors that influence the energy intake of snacks are gender, age, father's education, father's occupation, mother's education, mother's income, and subject's attitude (p<0.05). Factors affecting snack protein intake are gender, age, father's occupation, and mother's education (p<0.05). Factors influencing snack fat intake are gender, age, father's occupation, mother's education, and subject's attitude (p<0.05).
Keyword: pengetahuan gizi, sikap gizi, status gizi, kebiasaan jajan, siswa sekolah dasar

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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