Judul: Pengaruh Koreksi Pencaran dan Penggunaan Daerah Sidik Jari terhadap Klasifikasi Asal Geografis Tanaman Obat. (Studi Kasus : Tanaman Meniran dan Kumis kucing di Pulau jawa).
Abstrak: Tanaman obat adalah tanaman yang memiliki khasiat untuk pengobatan maupun pencegahan penyakit. Khasiat ini bersumber pada komponen kimia aktif dalam tanaman yang tidak sedikit dipengaruhi oleh faktor-faktor geografis. Oleh karena itu, perbedaan asal geografis tanaman obat memungkinkan adanya perbedaan karakteristik yang menyebabkan inkonsistensi khasiat sehingga upaya eksploratif untuk mengelompokkan jenis tanaman obat berdasarkan asal geografis penting dilakukan. Pengukuran yang seringkali digunakan adalah dengan teknik Fourier Transform Infra Red (FTIR). Namun, keluaran yang dihasilkan FTIR ini hanya berupa spektrum yang menunjukkan nilai serapan saat contoh disinari inframerah yang kontinu terhadap bilangan gelombang. Untuk memperoleh informasi yang menyeluruh dan akurat dari spektrum keluaran FTIR ini diperlukan teknik penanganan tertentu. Untuk mengantisipasi hal itu maka digunakan teknik Koreksi Pencaran. Sebagai studi kasus, pada penelitian ini digunakan data keluaran FTIR untuk sampel tanaman Meniran hijau dan Kumis Kucing berbunga putih yang diperoleh dari beberapa daerah di Pulau Jawa. Analisis Komponen Utama digunakan sebagai analisis perantara untuk mereduksi dimensi data yang sangat besar sehingga menjadi lebih mudah untuk diklasifikasikan dengan analisis gerombol. Hasil penggerombolan menunjukkan bahwa pada sampel tanaman Meniran maupun Kumis Kucing diperoleh sembilan gerombol karakteristik wilayah.
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Judul: Kendali Mutu Daun Kumis Kucing (Orthosiphon stamineus) Menggunakan Pengolahan Citra Dan Teknik Pengenalan Pola Secara Kemometrik
Abstrak: Kumis kucing (Orthosiphon stamineus) is one of medicinal plants that widely used as a traditional medicine. This research aims to use the spectral imaging as a method to quality control of kumis kucing leaves with diversity of quality based on the different from 3 areas of the plant that is correlated with the intensity of leaf color and pattern recognition techniques combine with chemometric PCA and PLSDA. Analysis of thin-layer chromatography with software image J shows the difference of quality active compound kumis kucing from 3 areas is Cigombong, Nagrak, and Pacet. The best standard model and polynomial using polynomial orde 2 (term 7). Samples from Nagrak have better quality than other areas. Model PCA produces 97% of the value of PC 1 and PC 2 3%. PLSDA analysis produces 3 models, namely models PLSDA from Cigombong (R2 kalibrasi= 0.8395, R2 prediksi= 0.8051), Nagrak (R2 kalibrasi= 0.8185, R2 prediksi= 0.7779), dan Pacet (R2 kalibrasi= 0.7603, R2 prediksi= 0.7392). This model has successfully predicted the kumis kucing leaves to approach the quality of different areas of the plant.
Keyword: Bogor Agricultural University, spectral imaging, quality control, PLSDA, PCA, kumis kucing

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable