Judul: Analisis keragaman genetik kerbau lokal (Bubalus bubalis) berdasarkan haplotipe DNA mitokondria
Abstrak: Mitochondrial genome (mtDNA) in vertebrate is a circular double stranded DNA molecule. It has a high mutation rate and strictly maternally inherited. This research was conducted to investigate the diversity among local buffalos based on mtDNA’s control region. Samples that were used in this research were native buffalos from 10 Jawa Tengah, 12 Nusa Tenggara Barat, 10 Sumatera Utara and 12 Banten. By in vitro amplification with Polymorphism Chain Reaction method (PCR) using a pair of primer AF22 and AF23, yielded 1145 base pair long. The selected region is a segment spanning the region of end part 3’ cyt-b gene through the partial of the 3’ control region (d-loop). The PCR product was digested with four restriction enzymes, which were AluI (AG↓CT), HaeIII (GG↓CC), HinfI (G↓AnTC) and MspI (C↓CGG). Two haplotypes were detected among 44 samples. The nucleotide diversity among these haplotypes is 0,17%. Haplotype 1 found in all sample locations, while haplotype 2 was only found in one sample from Sumatera Utara. Genetically, the buffalos from Jawa Tengah, Nusa Tenggara Barat and Banten guessed have a same origin heredity (D = 0,000) and they have a near kinship with Sumatera Utara’s buffalo (D= 0,0061).
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Judul: Analisis daerah d-loop dna mitokondria pada kerbau belang (bubalus bubalis) dengan menggunakan enzim restriksi (pcr- rflp)
Abstrak: Spotted buffalo is slaughtered buffalo for sacrifices in Tana Toraja funeral ceremonies. Spotted buffalo is believed gives security in the hereafter. Therefore at the funeral ceremony, many spotted buffaloes slaughtered. Generally, hundreds of buffaloes from every class sacrificed every year. Further studies about DNA genetic pattern from spotted buffalo is needed in order to kept it existence. One of them is mitochondrial DNA expecially D-loop region. The objective of this research was to know the the connection between D-loop fragment variation and skin color variation in B. bubalis as a basic information to kept the existence of spotted buffalo. Tha research was performed using PCR-RFLP technique. DNA amplification in Dloop region used spesific primers CTDF and CTDR was then cutted using restriction enzymes Hae III, Alu I, Rsa I, Hinc II, Bam HI and Eco RI. PCR amplification of D-loop generated same size DNA bands that were approximately 1361 bp. Cutting PCR product with Hae III, Alu I, Rsa I, and Hinc II generated DNA bands with same pattern (monomorphic). Meanwhile Bam HI and Eco RI did not generated any cutted DNA band. Cutting with Hae III generated DNA bands those were 636 bp, 166 bp, and 559 bp. Cutting with Alu I generated DNA bands those were 71 bp, 82 bp, 365 bp, 191 bp, 276 bp, 185 bp, dan 191 bp. Cutting with Rsa I generated DNA bands those were 284 bp dan 1077 bp. Cutting with Hinc II generated DNA bands those were 1203 bp dan 158 bp. Restriction site in D-loop fragmen in buffalo had no polymorphism. The prediction is first it was caused by the lack of restriction enzymes that have been used. Second it can be caused by restriction enzymes cutting site that being used were at the non hypervariable D-loop region. Third it can be caused by buffalo domestication which centered in Indonesia or because of buffalo introduction to Indonesia from one distribution source. So it can be said that those buffaloes came from the same ancestor. Because the result was monomorphic, it can be concluded that there was no clear connection between mitochondrial variation in D-loop fragmen with spotted skin color in buffalo.
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Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4