Judul: Community’s Perception of Mangrove Forest Management in The South Coastal Area of Bunaken National Park
Abstrak: Hutan mangrove merupakan ekosistem yang penting di kawasan pesisir selatan Taman Nasional Bunaken (TNB) baik bagi Balai Taman Nasional (BTN). Bunaken sebagai agen perlindungan penyangga taman nasional maupun bagi masyarakat desa penyangga yang menerima dampak tindakan pengelolaan. Persepsi masyarakat desa penyangga dapat merepresentasikan kinerja pengelolaan hutan mangrove yang dilakukan BTN Bunaken. Tujuan penelitian ini adalah menggambarkan persepsi masyarakat setempat terhadap pengelolaan hutan mangrove Wilayah Pesisir Selatan TNB yang mencakup aspek ekologi, sosio-ekonomi, dan kelembagaan. Penelitian dilakukan dengan menggunakan metode survai. Responden dipilih secara sengaja. Pada aspek ekologi, pengelolaan BTN Bunaken dinilai berhasil dalam meningkatkan kelimpahan dan kualitas habitat mangrove. Pada aspek sosio-ekonomi, pengelolaan BTN Bunaken memiliki kesesuaian yang baik dengan budaya desa dan konflik jarang terjadi. Pada aspek kelembagaan, masyarakat cenderung memiliki kepercayaan yang baik terhadap BTN Bunaken meskipun masyarakat menilai interaksi dan komunikasi langsung dengan pihak Balai perlu ditingkatkan.
Keyword: hutan mangrove, persepsi masyarakat, taman nasional bunaken, desa penyangga

Judul: Persepsi dan Kesediaan Masyarakat untuk Berpartisipasi dalam Pengelolaan Hutan Mangrove (Kajian di Dusun Sidofajar, Tanjungwidoro, Kabupaten Gresik)
Abstrak: Ekosistem mangrove menyediakan berbagai jasa lingkungan yang bermanfaat bagi kehidupan masyarakat pesisir. Ekosistem mangrove harus dijaga kelestariannya. Pengelolaan mangrove berbasis masyarakat (PMBM) menjadi salah satu alternatif pengelolaan ekologis hutan mangrove secara lestari. Pendekatan PMBM dalam praktiknya bervariasi di berbagai negara sehingga memberikan dampak yang berbeda pula. Penelitian ini bertujuan untuk menganalisis dinamika PMBM di Dusun Sidofajar yang penuh dengan kontestasi kepentingan aktor. Penelitian ini juga menganalisis pengaruh karakteristik individu terhadap persepsi masyarakat pada hutan mangrove dan kesediaannya untuk berpartisipasi dalam pengelolaan mangrove, serta pengaruh persepsi terhadap kesediaan untuk berpartisipasi ini. Metode yang digunakan dalam penelitian ini, yakni kuantitatif dan kualitatif dengan instrumen berupa kuesioner dan panduan wawancara mendalam serta observasi. Hasil penelitian menunjukkan karakteristik individu berpengaruh terhadap tingkat persepsi dan tingkat kesediaan berpartisipasi. Aspek pengetahuan berpengaruh signifikan terhadap tingkat kesediaan berpartisipasi., The mangrove ecosystem provides various environmental services that are beneficial to the lives of coastal communities. The mangrove ecosystem must be preserved. Community-based mangrove management (CBMM) is one of the alternative methods for sustainable ecological management of mangrove forests. The CBMM approach varies in practice across different countries, resulting in different impacts. This research aims to analyze the dynamics of CBMM in Tanjung Widoro Village, where there is a contestation of interests among actors. This study also examines the influence of individual characteristics on the community
Keyword: mangrove, Persepsi, partisipasi, Dinamika

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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