Judul: Studi Pustaka Pengaruh Aplikasi Probiotik untuk Pencegahan Penyakit Motile Aeromonas Septicemia pada Ikan Lele Clarias sp.
Abstrak: Penggunaan probiotik sebagai alternatif dari antibiotik untuk penyakit bakterial Motile Aeromonas Septicemia (MAS) yang disebabkan oleh bakteri Aeromonas hydrophila. Mekanisme kerja probiotik yaitu melekat pada mukosa usus, menghasilkan zat antibakteri, mengoptimalkan produksi enzim pencernaan, memelihara populasi mikroba di dalam usus, serta meningkatkan pemanfaatan pakan, pertumbuhan dan sintasan. Penelitian bertujuan mengumpulkan, merangkum dan mengkaji berbagai pustaka mengenai aplikasi probiotik dengan jenis dan dosis yang berbeda untuk mencegah penyakit Motile Aeromonas Septicemia (MAS) pada ikan lele (Clarias sp.). Penelitian dilakukan di Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor pada bulan Juli 2023 sampai Januari 2024 dengan metode studi literatur. Probiotik dapat mengubah mikrobiota usus, meningkatkan ketahanan terhadap penyakit, meningkatkan kelangsungan hidup, dan menjaga kualitas air selain memperkuat dan meningkatkan respon imun. Pemberian probiotik jika dibandingkan dengan perlakukan kontrol (tanpa probiotik) mampu meningkatkan respon imun humoral dan seluler serta imunitas ikan lele terhadap patogen Aeromonas hydrophila penyebab penyakit Motile Aeromonas Septicemia. Dosis probiotik untuk ikan lele sebaiknya berkisar antara 105 hingga 107 CFU mL-1 untuk memaksimalkan respon imunologi dan efek kelangsungan hidup sebesar 60–90%., The use of probiotics as an alternative to antibiotics for the bacterial disease Motile Aeromonas Septicemia (MAS) which is caused by the bacteria Aeromonas hydrophila. The mechanism of action of probiotics is that they attach to the intestinal mucosa, produce antibacterial substances, optimize the production of digestive enzymes, maintain the microbial population in the intestine, and increase feed utilization, growth and survival. The research aims to collect, summarize and review various literature regarding the application of probiotics with different types and doses to prevent Motile Aeromonas Septicemia (MAS) disease in catfish (Clarias sp.). The research was conducted at the Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural Institute from July 2023 to January 2024 using the literature study method. Probiotics can alter gut microbiota, increase disease resistance, improve survival, and maintain water quality in addition to strengthening and improving the immune response. Probiotic administration compared with the control treatment (without probiotics) was able to increase the humoral and cellular immune response as well as the immunity of catfish against the pathogen Aeromonas hydrophila which causes the disease Motile Aeromonas Septicemia. The probiotic dose for catfish should be in the range of 105 to 107 CFU mL-1 to maximize the immunological response and survival effect of 60–90%.
Keyword: Aeromonas hydrophila, catfish, probiotic, MAS

Judul: Application of Monospecies Synbiotic and Multispecies for Prevention of Aeromonas hydrophila Bacterial Infection in Sangkuriang Catfish (Clarias gariepinus)
Abstrak: Penyakit bakterial yang mengancam peningkatan produksi ikan lele salah satunya disebabkan oleh bakteri Aeromonas hydrophila. Penggunaan sinbiotik yang merupakan kombinasi probiotik dan prebiotik menjadi alternatif untuk pencegahan penyakit. Penelitian ini bertujuan untuk menguji pengaruh probiotik Bacillus cereus BR2 dengan prebiotik madu (sinbiotik monospesies), probiotik Staphylococcus lentus L1k dengan prebiotik madu (sinbiotik monospesies), probiotik B. cereus BR2 dan S. lentus L1k dengan prebiotik madu (sinbiotik multispesies) yang diberikan melalui pakan untuk pencegahan infeksi A. hydrophila pada ikan lele sangkuriang (Clarias gariepinus). Ikan uji dengan bobot awal (17,43 ± 0,22) g dipelihara selama 30 hari dan diberi pakan suplementasi, kemudian diuji tantang dengan bakteri A. hydrophila selama tujuh hari. Penelitian ini terdiri dari lima perlakuan dan tiga ulangan yaitu perlakuan A (BR2 1% + madu 0,5% + diuji tantang), B (L1k 1% + madu 0,5% + diuji tantang), C (BR2 0,5% + L1k 0,5% + madu 0,5% + diuji tantang), kontrol positif (tanpa probiotik dan prebiotik + diuji tantang), dan kontrol negatif (tanpa probiotik dan prebiotik + tanpa diuji tantang). Hasil penelitian menunjukkan sinbiotik monospesies dan multispesies mampu meningkatkan tingkat kelangsungan hidup, kinerja pertumbuhan, dan respons imun ikan lele untuk pencegahan infeksi A. hydrophila dengan hasil terbaik terdapat pada perlakuan sinbiotik multispesies., One of the bacterial diseases that threaten the increase in catfish production is Aeromonas hydrophila. The used of synbiotics which is a combination of probiotics and prebiotics is an alternative for disease prevention. This study aimed to examine the effect of probiotic Bacillus cereus BR2 with prebiotic honey (monospecies synbiotic), probiotic Staphylococcus lentus L1k with prebiotic honey (monospecies synbiotic), probiotic B. cereus BR2 and S. lentus L1k with prebiotic honey (multispecies synbiotic) through feed for the prevention of A. hydrophila bacterial infection to sangkuriang catfish (Clarias gariepinus). The fish size (17,43 ± 0,22) g were reared for 30 days and given supplementary feed, then challenged with A. hydrophila bacteria for seven days. This study consisted of five treatments and three replications i.e. treatment A (BR2 1% + honey 0,5% + challenged), B (L1k 1% + honey 0,5% + challenged), C (BR2 0,5% + L1k 0,5% + honey 0,5% + challenged), positive control (without probiotics and prebiotics + challenged), and negative control (without probiotics and prebiotics + no challenged). The results showed that monospecies and multispecies synbiotic could increase survival rate, growth performance, and immune respons of catfish to prevent of A. hydrophila infection with the best result found in the multispecies synbiotic treatment.
Keyword: Aeromonas hydrophila, Bacillus cereus BR2, Clarias gariepinus, Madu, Staphylococcus lentus L1k

Judul: Penyusunan Overlap Graph menggunakan Suffix Tree pada DNA Sequence
Abstrak: The DNA sequence assembly is very important in genome analysis. This step aims to reconstruct contigous DNA fragment (contigs) from short fragment by concatenating fragments based on the overlap region among them. This method employs overlap graph for solving the problems. In this graph, nodes represent fragments and edges represent overlap. This research developed a software for constructing overlap graph using suffix tree. The evaluation was conducted by measuring the number of nodes, number of edges, and the execution time in many values of minimum overlap. The minimum overlap is defined as the minimum length of substring which is considered as the overlap region between two fragments. The evaluation results showed that the number of nodes, the number of edges, and the execution time were increased with the decreasing of the minimum overlap value.
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