Judul: Characterization of Protease from Blood and Cloning of Factor C cDNA from Indonesian Horseshoe Crab (Tachypleus gigas)
Abstrak: The horseshoe crab blood has some medical benefits, especially for endotoxin detection, which is an ability thought to be due to proteases’ role. Therefore, this study aimed to characterize proteases derived and to isolate factor C from horseshoe crab blood collected from Banyuasin Estuary, South Sumatra, Indonesia. With the blood sample, 1 EU/ mL of endotoxin was detected using the LAL test method, but a concentration of 0.5 EU/mL was not detected. Furthermore, the molecular mass was between 60-75 kDa and 25-35 kDa. It also had protease activity at a molecular mass below 60 kDa, while the highest activity was at temperatures 70 °C as well as pH 11. Besides, the enzyme’s activity was mostly inhibited in the presence of PMSF, EDTA, and SDS, but potentially increased by Tween-20 and cofactors such as Mg2+ and Ca2+. Based on the results, the enzymes contained in horseshoe crab blood belong to serine proteases. Light chain factor C fragment has been isolated from the blood of Tachypleus gigas (990 bp) and ligated to pGem-T-easy. The light chain sequence from T.gigas is similar to T. tridentatus sequence with a percent identity of 98.38%.
Keyword: blood, endotoxin, factor C, horseshoe crab, serine protease

Judul: Karakterisasi aktivitas dialisat enzim protease fibrinolitik dari cacing tanah (Eisenia foetida) galur lokal
Abstrak: Commercially available thrombolytic drugs were usually administered intraveously and priced above consumer resources. Earthworm, in several Eastern Asia countries, was known for its thrombolytic properties. Its herbal drug had been used to increase blood flow and to treat leakage in blood vessel. Thrombolytical properties of fibrinolytic protease (lumbrokinase) enzyme from local strain Eisenia foetida had never been reported. This research was purposed to characterize its enzyme activity and its specificity to fibrin as substrate. Enzyme was purified through precipitation, dialysis, and column chromatography fractionation. The highest protease activity was obtained when the dialysate sample was incubated for 10 minutes in pH 8 at 60°C which activity was 0.239 Um . Spectrophotometry based analysis showed that lumbrokinase was able to degrade fibrin up to 30.9% compared to sample without enzyme addition. Working condition to obtain fibrinolytic activity was 10 minutes incubation in pH 8 at 60°C. Lumbrokinase samples electrophoresis at each step have molecular weight between 25.200 and 29.318 kD. The highest protein concentration was found in dialysate with 1.74 m m . Obtained dialysate has protein purity up to 72.28% higher than crude extract.
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Judul: Peranan Pesantren dalam Pembangunan Pedesaan
Abstrak: Pesantren (pondok atau pondok pesantren) adalah suatu lembaga pendidikan keagamaan Islam yang pada umumnya tumbuh di kalangan masyarakat pedesaan. Pertumbuhannya merupakan hasil refleksi pimpinan pesantren dan masyarakat terhadap kebutuhan masyarakat sekitar. Disamping sebagai lembaga pendidikan keagamaan, diharapkan pesantren dapat berperan sebagai lembaga sosial kemasyarakatan yang dapat membantu pemerintah dalam menyebar luaskan inovasi pembangunan kepada masyarakat, dan menggerakkan masyarakat agar berperan serta dalam pembangunan.
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