Judul: Analisis Keragaman Genetik Kelapa Sawit (Elaeis guineensis Jacq) Asal Jawa Barat Dengan Penanda RAPD (Random Amplified Polymorphic DNA)
Abstrak: Genetic diversity study is the way to clustering an individual in a population for various prospect such as plant improvement. Oil palm (Elaeis guineensis Jacq) in this research are a population of oil palm that still exist since Holland colony and estimate have resistances to Ganoderma sp. Genotype information from those were conducted by RAPD. This research aims to clustering 58 oil palm trees by RAPD method. The research consist of four stages that begin from DNA isolation, DNA amplification, genetic similarity analysis, and construction of phylogenetic tree by UPGMA (Unweighted Pair Group Method Arithmatic Mean) method in NTSYS. The result of DNA isolation were showed good band and 12000 bp in size. The DNA amplification with 20 random primer were resulted 1870 DNA band from 229 locus. About 95% among of them are polymorphic band. Similarity analysis was showed the genetic similarity coeficient value about 69% - 95%. The clustering at genetic similarity coeficient value 76% was divided the population into two big group. The increasing of genetic similarity coeficient value will be obtained more cluster. This genotype information can be used as references in oil palm improvement program
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Judul: Genetic Diversity Analysis of Oil Palm (Elaeis guineensis Jacq) Based on RAPD (Random Amplified Polymorphic DNA) Using Primer OPJ.
Abstrak: Random Amplified Polymorphic DNA (RAPD) dapat menjadi salah satu alternatif untuk menyeleksi tanaman kelapa sawit berproduktivitas tinggi dan tahan terhadap Ganoderma sp. Pada penelitian ini RAPD digunakan untuk mempelajari keragaman genetik 58 pohon kelapa sawit. Tahapan penelitian dimulai dari isolasi DNA, amplifikasi menggunakan primer OPJ, dan analisis filogenetik menggunakan metode UPGMA (Unweighted Pair Group Method Arithmatic Mean) dengan software NTSYS. DNA hasil isolasi memiliki kualitas yang baik berdasarkan analisis spektofotometri dan elektroforesis gel. Amplifikasi DNA dengan 20 primer seri OPJ menghasilkan 1.592 pita DNA dari 115 lokus, sebesar 68% diantaranya merupakan pita polimorfik. Analisis kemiripan genetik menunjukkan nilai koefisien kemiripan genetik sebesar 49% - 95%. Hasil pengelompokkan populasi kelapa sawit pada nilai kemiripan genetikRandom Amplified Polymorphic DNA (RAPD) dapat menjadi salah satu alternatif untuk menyeleksi tanaman kelapa sawit berproduktivitas tinggi dan tahan terhadap Ganoderma sp. Pada penelitian ini RAPD digunakan untuk mempelajari keragaman genetik 58 pohon kelapa sawit. Tahapan penelitian dimulai dari isolasi DNA, amplifikasi menggunakan primer OPJ, dan analisis filogenetik menggunakan metode UPGMA (Unweighted Pair Group Method Arithmatic Mean) dengan software NTSYS. DNA hasil isolasi memiliki kualitas yang baik berdasarkan analisis spektofotometri dan elektroforesis gel. Amplifikasi DNA dengan 20 primer seri OPJ menghasilkan 1.592 pita DNA dari 115 lokus, sebesar 68% diantaranya merupakan pita polimorfik. Analisis kemiripan genetik menunjukkan nilai koefisien kemiripan genetik sebesar 49% - 95%. Hasil pengelompokkan populasi kelapa sawit pada nilai kemiripan genetik
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Judul: Hydroxyapatite-Chitosan Composite Coating on Stainless Steel 316 to Improve Corrosion Resistance
Abstrak: Metals that were used in bone implantation should have good corrosion resistance that were not easily corroded when implanted into the body. Metal corrosion resistance of stainless steel 316 can be enhanced through the metal coating by hydroxyapatite-chitosan composite with composition ratio of hydroxyapatite (gram) and chitosan 3% (mL) 0.2:0, 0.2:0.1, 0.2:0.5, 0.2:1.0, and 0.2:1.5. Besides improving the corrosion resistance of metal, the presence of chitosan also maintain the position of hydroxyapatite on metal surfaces. Corrosion resistance increases with increasing amount of chitosan added and the best composition of hydroxyapatite-chitosan composite was 0.2:1.0.
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