Judul: Father's Perception, Child Educational Facilities, Child-Parent Communication, and Motivation for Continuing School of Adolescence Circular Migrant Worker Families
Abstrak: Anak pada keluarga pekerja migran sirkuler dengan kondisi hubungan orang tua dan anak yang terpisah memiliki tantangan tersendiri agar terus termotivasi melanjutkan sekolah. Penelitian ini bertujuan untuk menganalisis pengaruh persepsi ayah mengenai pendidikan anak, penyediaan fasilitas pendidikan anak, dan komunikasi anak-orang tua terhadap motivasi melanjutkan sekolah pada remaja keluarga pekerja migran sirkuler. Penelitian ini merupakan penelitian kuantitatif dengan metode penarikan contoh dengan non-probability sampling melalui teknik snowball sampling. Jumlah responden dalam penelitian ini adalah 40 anak dan 40 ayah menggunakan kuesioner melalui Google Form. Hasil penelitian menunjukkan pendidikan terakhir ayah, penyediaan fasilitas pendidikan anak, komunikasi anak-ibu berhubungan positif signifikan dengan motivasi melanjutkan sekolah. Pendidikan terakhir ayah dan komunikasi anak dengan ibu berhubungan signifikan positif dengan penyediaan fasilitas pendidikan anak. Penyediaan fasilitas pendidikan anak, frekuensi kepulangan, dan komunikasi anak-ibu memiliki pengaruh positif signifikan terhadap motivasi melanjutkan sekolah. Sementara itu, jumlah anak berpengaruh signifikan negatif terhadap motivasi anak dalam melanjutkan sekolah., Children in circular migrant worker families with separate parent-child relationships have challenges to keep them motivated to continue school. This study aims to analyze the effect of fathers' perceptions of children's education, provision of children's educational facilities, and parent-child communication on the motivation to continue schooling in adolescent families of circular migrant workers. This research is quantitative research with a sampling method with non-probability sampling through the snowball sampling technique. This study’s respondents were 40 children and 40 fathers using a questionnaire via Google Forms. The results showed that the father's last education, children's educational facilities, and child-mother communication had a significant positive relationship with motivation to continue school. The father's last education and the child's communication with the mother were significantly positively related to the child's educational facilities. Children's educational facilities, frequency of return, and child-mother communication significantly positively affect motivation to continue school. Meanwhile, the number of children significantly negatively affects children's motivation to continue school.
Keyword: educational facilities, circular migrant worker families, motivation to continue school, perception of education, parent-child communication

Judul: Pengaruh Dukungan Sosial dan Komunikasi Orang Tua-Anak terhadap Motivasi Melanjutkan Sekolah pada Remaja di Perdesaan
Abstrak: Motivasi menjadi salah satu hal yang penting dimiliki dalam diri remaja untuk melanjutkan pendidikan khususnya pada remaja di perdesaan. Tujuan penelitian ini adalah menganalisis pengaruh karakteristik anak dan keluarga, dukungan sosial, dan komunikasi orang tua-anak terhadap motivasi melanjutkan sekolah pada remaja di perdesaan. Penelitian ini menggunakan desain explanatory study dengan teknik pengambilan contoh non-probability sampling dengan jumlah 483 anak Sekolah Menengah Pertama di lokasi terpilih. Hasil penelitian menunjukkan bahwa dukungan sosial tertinggi didapat dari ibu dan komunikasi antara ibu-anak masuk pada kategori tinggi. Hasil penelitian juga menunjukkan peningkatan motivasi melanjutkan sekolah lebih besar peluangnya pada siswa perempuan. Selain itu, terdapat hubungan positif sangat signifikan antara variabel dukungan sosial dan komunikasi ibu-anak dengan motivasi melanjutkan sekolah. Berdasarkan model regresi yang dihasilkan, variabel usia ayah dan ibu, pekerjaan ayah dan ibu, dukungan sosial, dan komunikasi orang tua-anak memengaruhi motivasi melanjutkan sekolah pada remaja SMP di perdesaan., Motivation is one of the important things for teenager to continue their education, especially for teenager who lives in rural area. The purpose of this study was to analyze the influence of family and child characteristics, social support, and parent-child communication on motivation in continuing education among teenagers in rural area. This study used an explanatory design with nonprobability sampling technique with a total sample of 483 junior high school students. The result shows that the highest social support was obtained from the mother and communication between mother and child is in the high category. The result also shows that the increasing in motivation to continue education was greater in female students. Moreover, there is also a very significant positive relationship between social support, mother-child communication, and motivation to continue education. Based on regression model, the variables of father and mother's age, father and mother's occupation, social support, and parent-child communication influence the motivation to continue education among junior high school teenagers in rural area.
Keyword: motivation to continuing education, parent-child communication, rural area, social support, teenager

Judul: Characterization and Expression of Tomato infectious chlorosis virus Coat Protein Gene in Escherichia coli
Abstrak: Tomato infectious chlorosis virus (TICV) is a member of Crinivirus genus. TICV infects tomato crop in Garut and Cipanas, West Java Indonesia. Tomato initially showed bright interveinal yellowing symptoms. The coat protein gene (CP-TICV) was amplified by RT-PCR from total RNA extracted from infected tomato leaves and the amplified fragment was cloned and completely sequenced. The fragment was subsequently subcloned into the pET-21b expression vector. The recombinant plasmid was transformed to Escherichia coli strain BL21(DE3)pLysS to express the coat protein. The coat protein fused to a 6xhistag, was purified by affinity chromatography using a NiNTA spin column. The identity of the purified protein was confirmed by SDS-PAGE. In this experiment, 792 bp of CP-TICV gene of TICV virus has been successfully cloned, sequenced, and expressed in E. coli. Based on nucleotide sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain strain and 99% identity to North America, France, and California strains and based on amino acid sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain, North America, France strains and 99.2% identity to California strain. CP-TICV showed over expressed in E. coli when it is induced with 1 mM IPTG and incubated at 37oC. Purified CP-TICV-Histag recombinants protein sized 29 kDa based on SDSPAGE analysis.
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