Judul: Hubungan persepsi body dan image dan kebiasaan makan dengan status gizi pada atlet senam dan renang di sekolah atlet Ragunan Jakarta
Abstrak: Body image can be defined as the degree of satisfaction of the individual against himself physically that include size, shape, and appearance in general. Body image of adolescents is an important matter, because adolescence undergone many changes, both physically and psychologically. Because adolescence is one of the important stages in growth, perceptions of body image can be influenced by eating habits. Eating habits is what will affect nutritional status. This study aims to examine the relationship between perceptions of body image and eating habits with nutritional status of gymnastics and swimming athletes in the Ragunan Athletes School Jakarta. The research was conducted in May 2011 using cross-sectional study design. Purposively selected examples (n = 32 individuals) consist of 12 gymnastic athletes and 20 swimming athletes (average age 14.5 ± 1.8 years, average weight 52.5 ± 10.6 kg, and mean height 162.8 ± 10.5 cm). The results of this study shows that the level of nutrition knowledge is fair (40%) and the majority have normal nutritional status (96.9%). Most of the energy adequacy level examples are categorized highly deficit, while protein adequacy is normal. Iron and vitamin C adequacy are insufficient, whereas vitamin A adequacy is categorized sufficient. 68.75% of samples chose a positive perception of body image. The number of swimming athletes have more negative perceptions of body image than gymnastics athletes. There is a difference between the two samples on actual and ideal body shape. Spearman correlation test shows no correlation between family characteristics and sampel with perceptions of body image. Knowledge of nutrition didn’t have a significant correlation with the perception of body image (p = 0647 and r = 0084). While there is a significant correlation between actual body shape with perceptions of body image (p = 0.001 and r = 0718). The Spearman correlation test also examines the relationship between family characteristics and examples, knowledge of nutrition, eating habits, and perceptions of body image with nutritional status. Of the total surveyed, there is no correlation with nutritional status. Based on this study, there is a need of nutrition knowledge in samples which have a negative perception of body image in order to avoid misperceptions that could lead to over nutrition and even under nutrition.
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Judul: Hubungan antara Persepsi Body Image, Tingkat Kecukupan Gizi dengan Kelentukan dan Daya Tahan Atlet Senam di sekolah Atlet Ragunan Jakarta
Abstrak: Body image is the mental picture, perceptions, thoughts and feelings that one has to body size, body shape, and weight of the body leading to physical appearance. The purpose of this study was to determine the relationship between the perception of body image, nutrition level with flexibility and endurance athlete in school gymnastics athletes ragunan Jakarta. The design of this research study sesctional cross. Samples are taken purposively with the criteria or requirements that instance is students of Junior / Senior High School is a gymnastics athlete. There was no significant relationship between the perception of body image with energy adequacy level (p = 0.440) and protein adequacy level (p = 0.622). There is a relationship between the level of energy sufficiency and flexibility are significant (p=0.028) however, there is no significant relationship between the level of energy sufficiency and endurance (p=0.620). Relationship with the level of adequacy of protein with flexibility and endurance there is no significant relationship (p=0.220) and (p= 0.361).
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Judul: Isolasi Konstruksi dan Rekombinasi Gen Pengkode Reverse Transcriptase asal Simian Retrovirus-2 (SRV-2)
Abstrak: The availability of reverse transcriptase enzym as a valuable component for Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique has been providing by foreign companies. Therefore, it needs more cost and time to provide it. This study is an early step to produce in house reverse transcriptase rekombinant enzym from SRV-2. The purpose of this study was isolated, constructed, and rekombined SRV-2 reverse transcriptase gene to entry clone and destination vector. Isolation was done by PCR amplification technique using three pairs of primers, which are RT I (3282-3683), RTII (3261-3900), and RTIII (3169-4133). Construction and recombination in this study used Gateway method from Invitrogen. SRV-2 reverse transcriptase gene was isolated in this study with A-549 cell inoculated SRV-2 as source of virus. This gene amplify by PCR technique and resulted specific band for RTI 401 bp, RTII 639 bp, and RTIII 964 bp. Analysis of pENTR/SD/TOPO and pDEST17 indicated SRV-2 reverse transcriptase gene code has already inserted to this plasmid. This result indicated SRV-2 reverse transcriptase gene have isolated, constructed, and recombined to entry clone and destination vector.
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