Judul: Mutation Identification in Bone Morphogenetic Protein Receptor IB (BMPR-IB) and Bone Morphogenetic Protein 15 (BMP15) Genes are Associated with Proliferation in Prolific Garut Sheep.
Abstrak: Garut sheep is prolific sheep breed. A group of growth factor that is especially expressed in oocyte and ovarian follicle is grouped as oocyted-derived growth factor (ODGF), also known as the Tansforming Growth Factor β (TGFβ). Proliferation in sheep controlled by three main factor of TGFβ that are Bone Morphogenetic Protein Receptor IB (BMPR-IB), Bone Morphogenetic Protein 15 (BMP15) and Growth Differentiation Factor 9 (GDF9). Mutation identification in the 6th exon of BMPR-IB gene and 2nd exon of BMP15 gene was done in this research. Genotype composition of BMPR-IB gene in prolific Garut sheep consist of BB (11%), B- (69%), and – (20%). The frequency of B allele (mutant type) was 46% and -- allele (wild type) was 54%. Genotype composition of BMP15 gene was ++ (100%). These implied that there was no Galway mutation in BMP15 gene. Further detection of mutation on the 2nd exon of BMP15 gene using SSCP method was found four type of mutation denoted by A, B, C, D. All genotype had successfully detected; either as single or combined gene; were suggested that there was no correlation between gene and litter size.
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Judul: Polimorfisme Gen Fekunditas (BMPR1B dan BMP15) pada Kambing Kacang, Samosir dan Muara.
Abstrak: Indonesia memiliki potensi keanekaragaman hayati yang tinggi. Salah satunya adalah kambing lokal. Beberapa kambing lokal di Indonesia memiliki sifat unggul, antara lain bersifat prolifik. Sifat prolifik ini dikendalikan oleh gen fekunditas, antara lain Bone Morphogenetic Protein Receptor 1B (BMPR1B), Bone Morphogenetic Protein 15 (BMP15) dan Growth Differentiation Factor 9 (GDF9). Gen BMPR1B yang dikenal sebagai FecB terletak pada kromosom 6 dan diekspresikan oleh sel oosit dan sel granulosa dalam ovarium. Mutasi pada FecB yang berkaitan dengan sifat prolifik adalah substitusi A746G yang menyebabkan substitusi asam amino Q249R. Adapun gen BMP15 yang dikenal sebagai FecX terletak di kromosom X dan hanya diekspresikan di dalam sel-sel oosit. Beberapa alel mutan pada gen BMP15 yang berhubungan dengan sifat prolifik adalah FecXI (Inverdale), FecXH (Hanna), FecXB (Belclare), FecXG (Galway), FecXL (Lacaune) dan FecXR (Rasa Aragonesa). Penelitian ini bertujuan untuk mengidentifikasi keragaman genetik gen fekunditas (BMPR1B dan BMP15) pada kambing Kacang, Samosir dan Muara menggunakan metode PCR-RFLP dan sekuensing nukleotida.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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