Judul: Evaluasi Asam α-Lipoat Dalam Pakan Terhadap Kinerja Pertumbuhan Benih Ikan Gurami Osphronemus goramy
Abstrak: This study was performed to evaluate the supplementation of α-lipoic acid in diets on the growth performance of giant gourami juvenile. This study used the completely randomized design experimental method containing four diets treatments and four replications. The experimental diets comprised L0 as control (0.0 g/kg), L03 (0.3 g/kg), L06 (0.6 g/kg) and L09 (0.9 g/kg), which made as isonitrogenous and isocaloric diet. The experimental diets were given on the giant gourami juvenile (7.05 ± 0.08 g initial weight) for 60 days. The supplementation of α-lipoic acid on L06 treatment significantly improved the feed intake, specific growth rate, feed conversion ratio, protein retention, lipid retention, liver lipid, liver glycogen and superoxide dismutase enzyme. On the other hand, the highest malondialdehyde, blood triglycerides and hepatosomatic index were obtained from L0 treatment (Duncan: p < 0.05). The water quality parameters (temperature, pH, DO, nitrite, nitrate and TAN) had no significant difference among treatments. This study concludes that the supplementation of α-lipoic acid in diet improves the growth performance of giant gourami juvenile.
Keyword: α-lipoic acid, giant gouramy, growth

Judul: Physiology, Biochemistry, and Molecular Responses of Giant Gourami Juvenile Treated by Recombinant Growth Hormone
Abstrak: Ikan gurame (Osphronemus goramy Lac.) merupakan salah satu spesies target revitalisasi perikanan untuk tujuan konsumsi dalam negeri yang produksinya ditargetkan meningkat sekitar 4% per tahun pada tahun 2014. Kendala utama dalam mewujudkan target tersebut adalah laju pertumbuhan somatik ikan gurame yang relatif rendah, yang membutuhkan waktu sekitar 1,5 tahun untuk mencapai ukuran konsumsi (300-500 gram). Target produksi tersebut dapat diwujudkan antara lain melalui penerapan peningkatan kecepatan pertumbuhan ikan gurame menggunakan protein hormon pertumbuhan rekombinan (rGH). Protein rGH telah terbukti mampu memacu pertumbuhan ikan, udang, dan kekerangan, sehingga berdasarkan hal tersebut percobaan ini dilakukan. rGH ikan mas (rCcGH) dan ikan kerapu kertang (rElGH) diekspresikan pada bakteri Escherichia coli strain BL21 (DE3), diekstraksi dan kemudian diujikan ke benih ikan gurame melalui perendaman untuk memacu pertumbuhannya. Jenis rGH yang memberikan efek pertumbuhan terbaik digunakan pada penelitian selanjutnya untuk menganalisis efeknya terhadap proses metabolisme dan ekspresi gen insulin-like growth factors (IGFs) dan growth hormone receptors (GHRs). Pada penelitian ini juga dilakukan kloning gen IGF-1 dan perancangan primer spesifik IGFs dan GHRs ikan gurame untuk analisis kuantitatif polymerase chain reaction (qPCR). Pada tahap akhir penelitian dilakukan uji biokimia untuk mengevaluasi status nutrisi, potensi alergi, dan analisis performa otot daging dan beberapa organ dalam ikan gurame., Aquaculture production of giant gourami in Indonesia is targeted to increase by 4% per year. The main constraint to meet the goal is slow growth of giant gourami, Osphronemus goramy Lac, which takes nearly 1.5 years to grow to a market size of 300-500 g. The target can be achieved by using recombinant growth hormone (rGH) to enhance growth of juvenile. The research was divided into four chapters: (1) analysis of growth and biochemical responses of giant gourami juvenile immersed in recombinant Cyprinus carpio growth gormone with different dosages, (2) analysis of growth, metabolism, and molecular responses of giant gourami juvenile immersed by recombinant Epinephelus lanceolatus (rElGH) and Cyprinus carpio growth hormone (rCcGH), (3) cloning of insulinlike growth factor I (IGF-1) gene and designing specific primer for quatitative PCR (qPCR), (4) evaluation of nutritional properties, allergenic potential, and histological performance of giant gourami immersed with rElGH. The results showed that both rElGH and rCcGH could promote growth gain of juvenile giant gourami. The juveniles immersed by 0.03 mg L-1 rElGH was more efficient and effective to enhance the growth of giant gourami juvenile over 120 mg L-1 rCcGH. The rGH has gluconeogenesis effect, in the muscle and liver rGH stimulates triglyceride uptake by enhancing lipoprotein lipase (LPL) activity and its subsequent storage. The effects of rGH on carbohydrate metabolism may be mediated indirectly via the antagonism of insulin action.
Keyword: Giant gourami, growth hormone, immersion, growth, metabolism, IGF- 1, fatty acid, amino acid, histological performance, histamine

Judul: Sistem Pengklasifikasian Entitas pada Pesan Twitter Menggunakan Ekspresi Regular dan Naïve Bayes
Abstrak: The widespread use of social networks like Twitter encourages some institutions to find out the entities that are discussed and the sentiment toward the entities. This research aims to build a system that can extract entities in tweets and classify the sentiment from the tweets. Entity extraction process is done using regular expressions. Writing regular expressions can capture the diverse entities. Sentiment classification is performed using naïve Bayes classification with Multinomial and Bernoulli models. Sentiment in this research consists of three classes, namely positive, negative, and neutral. Before entering the classification, tweets are normalized by replacing the non-standard words with the standard ones. The test results show that regular expression is quite effective for the entity extraction process. As for the results of the classification, naïve Bayes method produces high accuracy (96.75% for Multinomial and 96.33% for Bernoulli). However, the accuracy obtained depends on the neutral class which amounts to 97.88% for Multinomial and 99.95% for Bernoulli, while the positive class are 85.66% and 33.13% and negative class are 33.67% and 0%.
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