Judul: Pemetaan Area Prioritas Restorasi Habitat Macan Tutul Jawa (Panthera pardus melas Cuvier 1809) di Taman Nasional Gunung Halimun Salak
Abstrak: Taman Nasional Gunung Halimun Salak merupakan perluasan dari kawasan Gunung Halimun dan Gunung Salak yang juga merupakan salah satu habitat macan tutul jawa. Keberadaan spesies ini sangat rentan terhadap perubahan luasan habitat akibat deforestasi. Penelitian ini bertujuan untuk mengidentifikasi area prioritas restorasi habitat macan tutul jawa di Taman Nasional Gunung Halimun Salak. Analisis data menggunakan perangkat lunak maximum entropy dan condatis. Hasil pemodelan kesesuaian habitat digunakan untuk menentukan lokasi sumber dan target, yaitu lokasi asal dan lokasi setelah pergerakan macan tutul jawa. Hasil analisis perangkat lunak Condatis berupa nilai flow atau nilai pergerakan suatu spesies. Perubahan nilai antara skenario Bussiness As Usual dan skenario restorasi menunjukkan area yang perlu dipertimbangkan untuk restorasi. Pendugaan area prioritas restorasi kelas tinggi sebesar 3912 ha, kelas sedang sebesar 86912 ha dan kelas rendah sebesar 63464 ha. Lokasi area prioritas restorasi kelas tinggi berada pada area kebun teh Cikaniki serta beberapa area di Resort Gunung Botol, Gunung Bodas, Gunung Bedil, Kancana, Cisoka dan Cibedug berupa semak, pertanian dan pemukiman.
Keyword: condatis, kesesuaian habitat, maximum entropy

Judul: Penggunaan Koridor sebagai Habitat Macan Tutul Jawa (Panthera pardus melas, Cuvier 1809) di Taman Nasional Gunung Halimun Salak.
Abstrak: Areal koridor Gunung Halimun Salak sangat penting sebagai saluran pertukaran genetik diantara spesies flora dan fauna. Namun sepertiga kawasan hutan koridor telah terganggu akibat aktivitas manusia. Penelitian ini dilakukan untuk mengetahui bagian-bagian kawasan koridor yang sesuai digunakan oleh macan tutul jawa. Data yang diambil meliputi keberadaraan macan tutul jawa, kondisi habitat, dan gangguan habitat. Hasil kamera jebakan ditemukan empat individu yang saling overlap pada wilayah jelajahnya. Ada 18 jenis satwa mangsa dan paling banyak pada hutan sekunder. Ketersediaan air masih tersebar diseluruh kawasan koridor. Nilai kesesuaian habitat diantaranya kelas rendah (11%), sedang (40%), dan tinggi (49%). Bentuk gangguan yang ditemukan adalah penebangan liar, perburuan liar, pembukaan lahan, pencarian rumput serta kegiatan motorcross.
Keyword: macan tutul, kesesuaian habitat, koridor

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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