Judul: Penggunaan Pupuk Majemuk Untuk Multiplikasi Nilam (Pogostemon cablin Benth) Secara In Vitro
Abstrak: Patchouli (P. cablin Benth) is an aromatic plant that produces essential oil. Patchouli oil is needed in chemical, perfume, cosmetics, and health industries. Patchouli seeds by conventional cutting procurement had several problems, therefore techniques of tissue culture was taken as a solution. Expensive culture medium has led to increased seed prices. In order to look for an alternative to replace the MS (Murashige-Skoog) basic medium, we tried to use compound fertilizer medium. The objective of this research was to determine the best concentration of compound fertilizer with the addition of 10% coconut water or BAP of 0.5 mg / l for the multiplication of patchouli in vitro. Data of growth parameters (number of leaves, number of shoots, shoot height) of patchouli in the medium was observed after 3 Months After Culture (MAC). The compound fertilizer medium at 2 g / l was the best concentration compared to the other concentrations, although the value was still far below the growth on MS medium.
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Judul: Perbanyakan In Vitro Klon Tanaman Nilam (Pogostemon cablin Benth.) Hasil Iradiasi Sinar Gamma
Abstrak: The aim of this experiment was to determine the proper composition of culture medium for multiplication and shoot elongation of patchouli clone. The experiment was devided into two parts. The first experiment was to determine the effect of PGRs on the multiplication of shoots and nodes of patchouli clone derived from 30 gray of gamma ray irradiation. The second experiment used three concentrations (full, ½ and ¼) of Murashige and Skoog medium (MS) in the elongation of patchouli shoots derived from 30 gray of gamma irradiation and control. Both experiments were prepared with completely randomized design (CRD) factorial and observed for eight weeks. The first experiment was divided into two parts. The first part used MS medium with 0, 0.1, 0.3, 0.5 and 0.7 mg/L of BAP without IAA and the second part used an addition of 0.1 mg/L IAA. In the first part the best number of adventitious buds was inducted in 0.5 mg/L of BAP concentration and node explant. In the second part was no difference found in the number of adventitious buds between the BAP concentrations and the explants. In the second experiment, ¼ MS media can be used for shoot elongation of both clones because it obtained the same root initiation, number of roots and increase in leaves number of with other concentrations. Stomata analysis was perfomed on 30 gray and control of patchouli leafs. The result showed the variety between clones through the difference between the density and size of the stomata.
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Judul: Cycle Hamilton pada Graf Lengkap, Graf Regular, dan Graf 2-Connected 4-Regular Berorder Kurang dari Sepuluh
Abstrak: Suatu graf disebut graf Hamilton apabila graf tersebut memuat cycle Hamilton, yaitu cycle yang melewati setiap vertex tepat satu kali. Menentukan keberadaan cycle Hamilton pada suatu graf dapat menggunakan syarat cukup yang berupa teorema-teorema yang telah diperkenalkan sejak tahun 1950-an. Beberapa syarat cukup yang dibahas dalam karya ilmiah ini adalah syarat cukup yang berhubungan dengan graf lengkap dan graf regular. Suatu graf lengkap berorder 𝑛������ ≥ 3 dan graf r-regular berorder 𝑛������ ≤ 2𝑟������ + 1 merupakan graf Hamilton, Karena kedua graf tersebut memuat cycle Hamilton. Selanjutnya, syarat cukup tersebut digunakan untuk menentukan keberadaan cycle Hamilton pada graf 2-connected 4- regular berorder kurang dari 10.
Keyword: graf Hamilton, graf regular, graf lengkap, graf 2-connected 4-regular