Judul: Analisis Polimorfisme DNA Tanaman Kunyit (Curcuma longa L.) dan Temulawak (Curcuma xanthorrhiza Roxb) Lokal Menggunakan Marka RAPD
Abstrak: Tanaman kunyit (Curcuma longa L.) dan temulawak (Curcuma xanthorrhiza Roxb) lokal Indonesia yang tersebar dan mudah ditemukan di wilayah Indonesia banyak digunakan sebagai obat tradisional. Kandungan kurkumin pada kedua tanaman tersebut memiliki banyak khasiat kesehatan, yaitu sebagai obat antiproliferatif dan antiinflamasi. Adanya polimorfisme DNA pada kedua tanaman tersebut di beberapa lokasi Pulau Jawa belum diidentifikasi. Oleh karena itu, perlu dilakukan analisis genetik untuk menentukan keragaman kedua tanaman tersebut. Salah satu teknik yang biasa digunakan untuk analisis keragaman genetik adalah PCR RAPD. Tujuan penelitian ini mengisolasi DNA tanaman kunyit dan temulawak dan menentukan pola genetik tanaman tersebut dengan PCR RAPD. PCR dilakukan dengan menggunakan 20 primer acak OPA, OPB, OPC, dan OPD (1-5). Ekstraksi DNA menggunakan metode modifikasi Zheng et al. 1995 pada 13 sampel daun temulawak dan kunyit. Hasil menunjukkan bahwa primer yang dapat mengamplifikasi DNA sampel, yaitu: primer OPA-1, OPA-3, OPA-4, OPC-1, OPC-2, OPC-4, dan OPD-4. Selain itu, hanya tiga sampel yang dapat teramplifikasi, yaitu: temulawak Wonogiri, temulawak Karang Anyar, dan kunyit Wonogiri. Sampel yang teramplifikasi memiliki pola genetik yang berbeda yang dihasilkan oleh setiap primer acak sehingga kedua puluh primer acak dapat digunakan sebagai marka untuk analisis fingerprint.
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Judul: Pola Keragaman Genetik Tanaman Kunyit (Curcuma longa Linn.) dan Temulawak (Curcuma xanthorrhiza Roxb.) Asal Pulau Jawa dengan PCR-RAPD
Abstrak: Turmeric (Curcuma longa Linn.) and Temulawak (Curcuma xanthorrhiza Roxb.) are Indonesian medicinal plants which nutritious for improving health. The pattern of genetic diversity and genetic relationship of these rhizome plants are not well known yet. The purpose of this research to analyze the pattern of genetic diversity and genetic relationship both plants using Polymerase Chain Reaction- Random Amplified Polymorhic DNA (PCR-RAPD). The sample used in this research include 6 turmeric plants from Bogor, Wonogiri, Karanganyar, Sukabumi and Ngawi; and 5 temulawak plants from Bogor, Wonogiri, Sragen, Karanganyar and Sukabumi. Stage of these research include DNA isolation; DNA amplification; and analysis pattern of genetic diversity and genetic relationship using NTSYS 2.02. The result of these research showed that isolation DNA was done successfully. The result of DNA isolation generally has a fairly good purity. The purity values of turmeric and temulawak in sequence were 1.093-1.417 and 1.044-1.544. The number of bands that arise from DNA amplification of turmeric and temulawak in sequence were 131 and 132 locus. The number of amplification bands mixed results and different sizes for each sample show the patterns of genetic diversity found in turmeric and temulawak plants. The closest genetic relationship showed by turmeric plants from Sukabumi and Ngawi; and temulawak plants from Bogor and Sukabumi. Genetic similarity coefficient ranged from turmeric plants and temulawak plants in sequence are 54%-92% and 55 %-83 %.
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Judul: Kekonsistenan Hamiltonian pada Gerak Gelombang Interfacial
Abstrak: The interfacial wave is an internal wave that occurs at boundary of two layers fluid. It appears because there is difference of mass density between the two layers. The objective of this thesis is to formulate the motion of internal wave with flat base and flat as well as free upper bound surface. The motion equation obtained is a Hamiltonian system. The Hamiltonian in this system is expressed by variable of wave deviation and horizontal component of potential velocity. Under assumption that interfacial wave moves only in one direction, it can be shown that the Hamiltonian depends on the solution of the wave motion equation. The coefficients of the equation depend on the physical characteristics of the two layers of fluid, i.e. thickness and mass density. The numerical results show that Hamiltonian in the system is constant. It is consistent with Hamiltonian characteristic, which does not depend on time.
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