Judul: Identifikasi dan Karakterisasi Plasmodium Pada Beruk (Macaca nemestrina) di Pusat Studi Satwa Primata IPB
Abstrak: Beruk (Macaca nemestrina) merupakan salah satu satwa primata yang saat ini populasinya di alam mengalami penurunan. Penyakit yang dapat menyerang satwa, khususnya pada primata adalah malaria yang disebabkan oleh Plasmodium sp.. Penelitian ini bertujuan mempelajari morfologi Plasmodium sp. pada beruk (Macaca nemestrina) melalui teknik ulas darah. Penelitian ini juga membedakan presentase sel darah putih (leukosit) antara beruk yang terinfeksi dan yang tidak terinfeksi Plasmodium sp. Sampel darah diambil dari total 24 ekor beruk pada satu populasi penangkaran untuk pemeriksaan Polymerase Chain Reaction (PCR) dan dilanjutkan dengan pemeriksaan hematologi. Data dianalisis secara deskriptif dan statistik. Analisis deskriptif pada masing-masing sampel menunjukkan darah beruk yang terinfeksi Plasmodium sp. sudah mencapai stadium skizon. Analisis statistik menunjukkan tidak ada perbedaan nyata antara beruk yang positif dan negatif Plasmodium sp.., Southern pig-tailed macaque (Macaca nemestrina) is one of the primates whose population in nature is currently decreasing. One of the diseases that can attack animals, especially primates, is malaria caused by Plasmodium sp.. This study aims to determine the morphology of Plasmodium sp. on macaques (Macaca nemestrina) through the blood smear technique. In addition, this study also differentiates the percentage of white blood cells (leukocytes) between infected and uninfected monkeys with Plasmodium sp. Blood samples were taken from 24 macaques in one captive population for Polymerase Chain Reaction (PCR) examination and continued with hematological examination. Data collection was carried out by examining a blood sample in the form of a blood smear. Data were analyzed descriptively and statistically. A Descriptive analysis on each sample showed the blood of monkeys infected with Plasmodium sp. had reached the schizont stage. Statistical analysis showed that there was no significant difference between positive and negative Plasmodium sp..
Keyword: beruk, malaria, Plasmodium sp., sel darah putih, ulas darah

Judul: Overview of White Blood Cell in Macaca fascicularis with Plasmodium spp Infection Status
Abstrak: Keadaan hutan Indonesia sangat cocok sebagai tempat berkembangbiak monyet ekor panjang (Macaca fascicularis) dan vektor malaria (Plasmodium spp.), serta pemanfaatan area hutan oleh manusia mungkinkan terjadinya penyebaran penyakit malaria pada manusia melalui monyet. Plasmodium spp. ditularkan dari individu ke individu lainnya melalui gigitan nyamuk Anopheles. Pada penelitian ini penggunaan monyet ekor panjang sebagai hewan model dikarenakan memiliki anatomi dan fisiologi yang sama dengan manusia. Sampel yang digunakan berupa whole blood dari Macaca fascicularis, selanjutnya dibuat ulasan darah diatas gelas objek dan diwarnai dengan Giemsa. Sampel tersebut telah dikonfrimasi status infeksi Plasmodium spp. melalui uji Polymerase Chain Reaction (PCR), selanjutnya ulasan tersebut dilakukan perhitungan diferensial leukosit. Data yang didapat dianalisis secara deskriptif dan diolah secara statistik dengan uji Mann Whitney. Hasil perhitungan diferensial leukosit menunjukkan adanya perbedaan yang nyata pada jenis neutrofil batang antara monyet ekor panjang yang positif infeksi Plasmodium spp. dengan kontrol negatif. Hasil pengamatan menunjukkan fase infeksi Plasmodium spp. yang ditemukan pada sampel darah adalah fase tropozoid, fase skizon, dan fase gametosit., The condition of Indonesia's forests is very suitable as a breeding ground for long-tailed monkeys (Macaca fascicularis) and malaria vectors (Plasmodium spp.), and the use of forest areas by humans allows the spread of malaria to humans through monkeys. Plasmodium spp. transmitted from individual to individual through the bite of the Anopheles mosquito. In this study, the use of long-tailed monkeys as animal models is due to having the same anatomy and physiology as humans. The sample used was whole blood from Macaca fascicularis, then blood smear preparations was made on an object glass and stained with Giemsa. The sample has confirmed the infection status of Plasmodium spp. through the Polymerase Chain Reaction (PCR) test, then calculated the differential leukocyte count. The data obtained were analyzed descriptively and statistically with the Mann Whitney test. The results of the differential leukocyte count showed a significant difference in the types of stem neutrophils between long-tailed monkeys that were positive for Plasmodium spp. infection with negative control. The results showed that the infection phase of Plasmodium spp. The results showed that the infection phase found in blood samples are trophozoite stage, schizont phase, and gametocyte phase.
Keyword: leukosit, monyet ekor panjang, Plasmodium spp, ulas darah

Judul: Hydroxyapatite-Chitosan Composite Coating on Stainless Steel 316 to Improve Corrosion Resistance
Abstrak: Metals that were used in bone implantation should have good corrosion resistance that were not easily corroded when implanted into the body. Metal corrosion resistance of stainless steel 316 can be enhanced through the metal coating by hydroxyapatite-chitosan composite with composition ratio of hydroxyapatite (gram) and chitosan 3% (mL) 0.2:0, 0.2:0.1, 0.2:0.5, 0.2:1.0, and 0.2:1.5. Besides improving the corrosion resistance of metal, the presence of chitosan also maintain the position of hydroxyapatite on metal surfaces. Corrosion resistance increases with increasing amount of chitosan added and the best composition of hydroxyapatite-chitosan composite was 0.2:1.0.
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