Judul: Uji keamanan lingkungan vaksin DNA anti-KHV: uji in vivo dengan bakteri Aeromonas hydrophila pada periode interaksi berbeda
Abstrak: Environmental safety assessment of the DNA vaccine Anti-KHV is required before it applied widely. This research aimed to examine interaction possibility of anti-KHV DNA vaccine and bacteria in fish body within 1, 7, and 20 days post interaction subsequently. DNA vaccine (12.5 μg/100 μL PBS) and bacteria Aeromonas hydrophila (106 cfu/mL) were injected to common carp (6-8 cm in total body length). Treatment A fish was injected with vaccine first and 24 hours afterward with bacteria. Treatment B fish were injected vaccine and bacteria simultaneously. Treatment C fish was injected bacteria first and 24 hours afterward with vaccine. Treatment D fish was injected with bacteria only. At day 1, 7, and 20, bacteria were isolated from blood and kidney using TSA media containing ampicilin (0.05 ppt). The results showed that several isolates grew in the bacterial culture media. However, DNA analysis revealed that no isolates contained DNA vaccine. Those indicates that no DNA vaccine transferred to A. hydrophila; hence it concluded that DNA vaccine is safe for environment.
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Judul: Deteksi Transmisi Vaksin Dna Anti-Khv Pada Bakteri Di Media Budidaya Ikan Mas
Abstrak: KHV merupakan penyakit menular yang menyerang ikan mas dalam berbagai stadia, dapat mengakibatkan kematian 80-100%. Salah satu cara penanggulangan serangan KHV adalah dengan penggunaan vaksin DNA. Vaksin DNA merupakan produk rekayasa genetika yang dikhawatirkan dapat melakukan transmisi pada bakteri di perairan dan mempengaruhi keragaman hayati. Penelitian ini bertujuan untuk mendeteksi transmisi vaksin DNA anti-KHV ke bakteri di air pemeliharaan ikan mas. Vaksinasi dilakukan menggunakan metode perendaman, dan pemberian melalui pakan. Bakteri dari air vaksinasi melalui perendaman dan akuarium pemeliharaan ikan mas diisolasi pada 6 jam, hari ke-4, dan hari ke-7 pascavaksinasi, sedangkan bakteri dari kolam diisolasi setelah 6 jam, hari ke-4 dan hari ke-7 ikan dipelihara di kolam. Bakteri tersebut disebar sebanyak 50 μL pada media kultur mengandung ampisilin (50 μg/μL). Bakteri yang tumbuh pada media TSA yang mengandung ampisilin sebanyak 103 bakteri dan yang teridentifikasi hingga tingkat spesies dengan menggunakank kit API ada 11 bakteri, yakni 2 Aeromonas hydrophila, 4 Bacillus cereus, 2 B. subtilis, 2 Staphylococcus auricularis dan 1 Enterobacter sakazakii. Keberadaan vaksin DNA dalam bakteri diuji menggunakan metode cracking, dan PCR. Hasil cracking menunjukkan tidak adanya bakteri yang mengandung vaksin DNA anti- KHV. Analisis bakteri dengan metode PCR menunjukkan bahwa bakteri tidak dapat meng-uptake atau mengambil vaksin DNA anti-KHV dari lingkungan, sehingga dapat disimpulkan bahwa vaksin DNA anti-KHV tidak ditransmisikan ke bakteri media pemeliharaan ikan mas.
Keyword: KHV, Vaksin DNA, transmisi, bakteri dalam air, ikan mas

Judul: Tree berdiameter lima adalah graceful
Abstrak: This manuscript presents some proofs that every tree of diameter five is graceful. A graceful tree is a graph which has a graceful labelling. This labelling assigns absolute difference of connected vertices, such that the resulting edge labels are mutually distinct. There are three theorems to be considered. First theorem proves that every tree of diameter four is graceful. Second theorem proves that every tree of diameter five is graceful or nearly graceful. The third theorem improves the second theorem and shows that every tree of diameter five is nearly graceful, which can be transformed into graceful using some certain patterns.
Keyword: Graf, Pelabelan graceful, Bogor Agricultural University, Institut Pertanian Bogor, IPB