Judul: Aplikasi PCR berbasis marka sub1 (AEX1 dan RM219) pada seleksi padi BC1F1 Ciherang-Sub1
Abstrak: Submergence stress is the third most important limitation to rice production from 42 biotic and abiotic stresses. FR13A has adapted to excessive flooding environment by submergence tolerance is controlled by a single major quantitative trait locus (QTL) on chromosome 9, named Sub1. However, these varieties are susceptible to diseases and insect pests. It is therefore an attractive research proposition to transfer Sub1 gene from traditional cultivars into more productive varieties. In this study, the Ciherang was used as host, and F1 Ciherang-Sub1 was used as donors Sub1. The purpose of this study were to generate BC1F1 Ciherang-Sub1 varieties from backcross between F1 Ciherang-Sub1 and Ciherang by site-directed crossing methods. Selection of BC1F1 was done by submergence test and PCR analysis based Sub1 marker. BC1F1 Ciherang-Sub1 was succed in producing and contain Sub1 gene are obtained 16 BC1F1 Ciherang/Swarna-Sub1 and 22 BC1F1 Ciherang/IR64-Sub1 plants. Results of PCR amplification based AEX1 showed DNA band for Sub1A gene at 231 bp. The rices were containing DNA band belong to submergence tolerance rices. RM219 marker could distinguished between Ciherang, Sub1 donors, and BC1F1 Ciherang-Sub1 with DNA fragment at 204 bp and 194 bp. However, the different is too small, it is ranges of 5-10 bp until difficult to determine clearly heterozygous of BC1F1 Ciherang-Sub1.
Keyword: 

Judul: Identifikasi dan Karakterisasi Gen Toleran Genangan Populasi Padi BC4F1 Ciherang-Sub1
Abstrak: Submergence stress is one of the inhibitor factor at rice productivity. Therefore, in order to improve food security, the rice of submergence-tolerant need to be developed. Introduction of gene related to submergence-tolerant (Sub1) was done by site-directed crossing between Ciherang rice as host with rice IR64-Sub1 and Swarna-Sub1 as Sub1 donor. The aim of this study was to identify and characterize BC4F1 Ciherang-Sub1 that resulted from backcross between BC3F1 Ciherang-Sub1 and Ciherang. The existence of Sub1 gene was detected by using PCR analysis based on molecular marker that concerned Sub1 gene. Stages of research was started from planting of BC4F1 Ciherang-Sub1 seed, testing of submergence tolerance, isolation of rice DNA, measuring of concentration and purity of rice DNA, and PCR analysis with AEX1 and RM219 markers. The test results of the submergence showed that 15 plants of BC4F1 Ciherang/Swarna-Sub1 and 21 BC4F1 Ciherang/IR64-Sub1. PCR results with markers AEX1R and AEX1F showed that the DNA band sized 231 bp, while the results of PCR with the combined markers AEX1R and RM219F can distinguish heterozygote between Ciherang rice, Swarna-Sub1 and IR64-Sub1.
Keyword: Padi BC4F1, padi IR64-Sub1

Judul: Konsumsi Pangan dan Status Gizi Ibu Hamil di Kecamatan Danurejan, Kodya Yogyakarta, Daerah Istimewa Yogyakarta
Abstrak: Hasil penelitian menunjukkan bahwa rata-rata konsumsi energi per orang per hari adalah 1 525 Kalori, konsumsi protein 45,l g, konsumsi zat besi 12,8 mg dan konsumsi vitamin A 2 264 SI. Rata-rata kecukupan energi per orang per hari adalah 2 213 Kalori, kecukupan protein 75,6 g, kecukupan zat besi 29,O mg dan kecukupan vitamin A 4 500 SI.
Keyword: