Judul: Isolasi dan Identifikasi Staphylococcus aureus pada Vagina Sapi Simmental yang Mengalami Kawin Berulang
Abstrak: Staphylococcus aureus merupakan salah satu bakteri penyebab infeksi sekunder pada sapi yang mengalami kawin berulang (repeat breeding, RB). Kawin berulang dapat menyebabkan penurunan angka kebuntingan dan kelahiran pada sapi. Tujuan dari penelitian ini adalah mengisolasi dan mengidentifikasi Staphylococcus aureus dari vagina sapi Simmental yang mengalami RB dari BPTU-HPT Padang Mengatas. Sebanyak lima spesimen bilasan vagina dikoleksi dari lima ekor sapi Simmental yang mengalami RB. Metode yang digunakan untuk identifikasi bakteri pada penelitian ini, yaitu pewarnaan Gram dan uji biokimia. Spesimen dikultur pada media blood agar (BA) dengan teknik gores T, lalu dilakukan pewarnaan Gram dan dilanjutkan dengan uji biokimia. Hasil penelitian menunjukkan bahwa tidak ditemukan bakteri Staphylococcus aureus dari kelima spesimen bilas vagina sapi yang diidentifikasi. Adapun bakteri yang teridentifikasi, yaitu bakteri genus Micrococcus dan Bacillus. Kata kunci: kawin berulang, sapi Simmental, Staphylococcus aureus, Staphylococcus aureus is a bacteria’s causes secondary infection toward the repeated breeding (RB) of cows. repeated breeding could affect the decline of pregnancy rate for the cows. This study aimed to isolate and identify the Staphylococcus aureus in Simmental’s vagina through the RB in BPTU-HPT Padang Mengatas. There were five specimens of vaginal douching collected from five Simmental cows had been through repeated breeding. There were two methods used in this research to identify the bacteria, those were Gram Staining and Biochemical test. The specimens were cultivated within the media of Blood Agar (BA) by using T-Streak technique. The next step was Gram staining, and continued by biochemical testing. The result showed that there were no Staphylococcus aureus bacteria found from those identified specimens. On the other side, the bacteria identified from those specimens were genus Micrococcus and Bacillus. Keywords: repeated breeding, Simmental cows, Staphylococcus aureus
Keyword: repeated breeding, Simmental cows, Staphylococcus aureus

Judul: Identification of Staphylococcus aureus from Vagina of Limousine Cattle with Repeat Breeding in BPTU-HPT Padang Mengatas
Abstrak: Repeat breeding (RB) merupakan satu diantara kasus gangguan reproduksi yang umum terjadi pada sapi yang dapat menyebabkan penurunan fertilitas induk dan angka kebuntingan. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri Staphylococcus aureus yang diduga menjadi satu diantara penyebab kasus repeat breeding pada sapi Limousin di BPTU-HPT Padang Mengatas. Sampel yang digunakan sebanyak lima ekor sapi Limousin yang mengalami RB. Spesimen berasal dari cairan vagina sapi yang mengalami RB. Metode uji yang digunakan untuk identifikasi bakteri adalah kultur, pewarnaan Gram dan uji biokimia. Hasil uji identifikasi menunjukkan bahwa pada lima sampel yang diuji tidak ditemukan bakteri Staphylococcus aureus. Namun, ditemukan bakteri lain diantaranya Micrococcus sp., Bacillus sp., Escherichia sp., Achromobacter sp., dan Alcaligenes sp. Kata Kunci: gangguan reproduksi, repeat breeding, sapi Limousin, Staphylococcus aureus, Repeat breeding (RB) is one of the common cases of reproductive disorders in cattle that could lead to decrease of calf birth and conception rate. The objectives of this study was to isolate and identify the Staphylococcus aureus which was suspected as one of the causes of repeat breeding cases on Limousine cattle in BPTU-HPT Padang Mengatas. The samples used were five Limousine cattle which suffer from RB. The specimens came from vaginal discharge of the cattle which suffer from RB. The test method used to identify bacteria are culture, Gram stain and biochemical test. The results of the identification test showed that there is no Staphylococcus aureus bacteria found over the five samples tested. However, there were other bacteria found including Micrococcus sp., Bacillus sp., Escherichia sp., Achromobacter sp., and Alcaligenes sp. Keywords : Limousine cattle, repeat breeding, reproductive disorder, Staphylococcus aureus
Keyword: repeat breeding, Staphylococcus aureus, Gangguan reproduksi, sapi Limousin

Judul: Hybrid solar cells of ZnO nanoparticles/chlorophyll was modified metal ions (Zn2+ and Cu2+ )
Abstrak: Zinc Oxide (ZnO) is a n-type semiconductor with a wide the energy band (Band Gap) is 3.37 eV at room temperature and 3.34 eV at low temperature with a large exiton binding energy is 60 meV. ZnO has been synthesized by hydrothermal method, resulting in a decrease in particle size over the duration of the hydrothermal changes. Crystallographic structure of ZnO were characterized using XRD, has shown the characteristic pattern of a hexagonal wurtzite structure, the crystallite size increases to changes in particle size. Changes in particle size has also led to a change in the absorption peak of ZnO as has been demonstrated posted under test using the optical properties of UV-Vis spectrophotometer. Changes of this uptake has also led to changes in the energy band gap, which increases its band gap energy of the particle size. In electronic applications, especially in solar cells. Chlorophyll is considered less effective, because of their very nature are easily degraded as a result of several reactions which are acid, lighting and temperature. The results of the stability test using a halogen lamp 34 W/m2 for 5 hours, that the substitution of chlorophyll with metal ions Zn2+ and Cu2+ has been shown to form more stable than natural chlorophyll. The characterization of the optical properties, the combination of ZnO / chlorophyll in the form of hybrid films has led to a shift in the absorption region of ZnO becomes more widely from the UV to Visible. ZnO/chlorophyll has absorption range of 350-515 nm, ZnO / Zn-feofitin 332-401 nm and 332-632 nm for Cu-feofitin. The combination of ZnO/chlorophyll and CuSCN as a solid electrolite in hybrid solar cell, a modified chlorophyll with metal ions Zn2+ and Cu2+ has been shown to form a more stable I-V curve. It is characterized by its fill factor value greater than natural chlorophyll. Nevertheless, solar cells with natural chlorophyll has been able to produce higher efficiency values of chlorophyll were substituted.
Keyword: Hydrothermal, ZnO/Chlorophyll modified, Hybrid Solar Cell