Judul: Pertumbuhan, Efisiensi Pakan, Kelangsungan Hidup, dan Tingkat Stres Ikan Mas Cyprinus carpio L Transgenik Hormon Pertumbuhan pada Pemeliharaan Suhu Rendah
Abstrak: Growth hormone has been reported to be able to increase fish adaptability to the environment. The purpose of this research was to evaluate the growth performance, feed efficiency, survival rate, and stress level of growth hormone transgenic common carp reared in low temperature (15±0.3 °C). The design used in this research was completely randomized design consisted of 2 treatments (transgenic and non-transgenic) and 3 replications. Transgenic and non-transgenic common carp were reared in 30x25x30 cm3 sized tanks with stocking density of 10 fishes/tank for 30 days. The results showed that there was no significant differences in growth between transgenic and non-transgenic fishes (p>0.05). The survival rate of both transgenic and non-transgenic fishes was 100%. There was also no significant difference found in total feed consumption of transgenic and non-transgenic fishes (p>0.05) and it was 3.43±0.01 g for transgenic and 3.71±0.16 g for non-transgenic. Similar result was also found in feed efficiency between transgenic and non-transgenic fishes (p>0.05), 86.85±14.03% for transgenic and 70.57±8.36% for non-transgenic. Resistance against stress of transgenic fish was presumed higher compared to non-transgenic fish indicated by significant difference in their blood glucose level (p<0.05). Glucose level of transgenic fish (51.56±7.40 mg/dL) was lower compared to non-transgenic fish (90.78±12.44 mg/dL). It could be concluded that transgenic common carp reared in low temperature was presumed has higher resistance against stress compared to non-transgenic fish, meanwhile the survival rate, growth, and feed efficiency of transgenic and non-transgenic common carp were similar.
Keyword: Bogor Agricultural University (IPB), transgenic., low temperature, growth, common carp

Judul: Introduksi dan Ekspresi Gen Hormon Pertumbuhan pada Ikan Mas Transgenik
Abstrak: Common carp Cyprinus carpio is one of the economically valuable aquaculture fish species in Indonesia. This research was aimed to produce transgenic common carp that grow rapidly by over-expressing the tilapia growth hormone (tiGH) gene. Trangenic common carp were produced by sperm-mediated gene transfer using electroporation method. In this study, two electroporation programs that developed by other researcher were applied to obtain a suitable electroporation method for common carp. The electroporated sperm were then used to fertilize eggs to produce F0 transgenic fish. Transgenic F0 carrying tiGH in their sperm were crossed with non-transgenic fish to produce transgenic first (F1) generation. Identification of transgenic fish was performed by PCR method using specific primer for tiGH gene. Expression of tiGH was analyzed using RT-PCR method and growth measurement at F1 fish generation. The results showed that electroporation of sperm using 50 Volt/cm, and DNA concentration of 25 ng/μl was produced higher percentarge of fish carrying tiGH in their gamet compared to that of another electroporation program (40 Volt/cm, DNA concentration of 50 ng/μl). Five sexually matured males F0 transgenic fish were selected and mated with non-transgenic to produce F1 generation. Percentage of F1 progenies carrying tiGH was 47,2-66,7%. Variated measure of F1 genereations was 10,1->12,0 cm size (22,5%-42,9%), 8,1->10,0 cm size (14,3%-42,9%), 6,1->8,0 cm size (11,9%-29,2%), 4,1->6,0 cm size (0,0%-39,3%). Increasing of growth of F1 was about 1,4-1,87 times for non transgenic fish. Survival of transgenic and non-transgenic F1 was generally similar. RT-PCR analysis showed that the F1 transgenic fish have tiGH gene expression in caudal fin, while no expression in non-transgenic fish were found. Thus, the increased body weight of F1 transgenic common carp was associated with expression of tiGH gene, and this fish can be used as broodstock for the production of fast-grow stable transgenic common carp.
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Judul: Determinan dan Invers Matriks Leslie Ganda dengan Formula Schur
Abstrak: Matriks Leslie ganda merupakan bentuk umum dari matriks Leslie, matriks companion, dan matriks companion ganda. Bentuk khusus komplemen Schur dapat digunakan pada formula Schur untuk mendapatkan rumus determinan dan invers. Tujuan dari penulisan karya ilmiah ini adalah mengkaji secara teoritis pembuktian formulasi determinan dan invers matriks Leslie ganda berdasarkan formula Schur. Pembuktian formula determinan dan invers matriks Leslie ganda dilakukan dengan mensubstitusi formula determinan dan invers matriks persegi menggunakan komplemen Schur., The doubly Leslie matrix is generalized of the Leslie matrix, the double companion matrix, and the companion matrix. The unique form of Schur complement can be used in Schur’s formula to obtain a formula of the determinant and inverse of doubly Leslie matrix. In this article, the proof of those formulation are discussed theoretically based on Schur’s formula. Proof of the determinant and inverse formulation perform by substitute the determinant and inverse of square matrix with Schur Complement.
Keyword: Komplemen Schur, Formula Schur, Determinan, Invers, Matriks Leslie Ganda