Judul: Pengaruh Interaksi Keluarga, Kepadatan dan Kesesakan terhadap Kesejahteraan Subjektif Remaja.
Abstrak: Fenomena laju pertumbuhan penduduk dapat menyebabkan perubahan kepadatan dan kesesakan di lingkungan tempat tinggal. Lingkungan yang mendukung dan interaksi keluarga yang baik akan menentukan kebahagiaan. Penelitian ini bertujuan untuk menganalisis pengaruh interaksi keluarga, kepadatan, dan kesesakan dilingkungan tempat tinggal terhadap kesejahteraan subjektif remaja. Penelitian ini menggunakan desain cross sectional study di Kelurahan Panaragan, Bogor Tengah. Contoh penelitian melibatkan 120 keluarga lengkap yang memiliki anak remaja, dipilih dengan stratified disproportional random sampling. Uji korelasi menunjukkan adanya hubungan positif antara interaksi keluarga dengan kesejahteraan subjektif remaja, sementara kesesakan memiliki hubungan negatif dengan kesejahteraan subjektif remaja. Uji regresi menunjukkan bahwa interaksi keluarga dan jumlah ruangan berpengaruh positif terhadap kesejahteraan subjektif remaja, sementara kesesakan berpengaruh negatif terhadap kesejahteraan subjektif.
Keyword: interaksi keluarga, kepadatan, kesesakan, kesejahteraan subjektif remaja

Judul: Pengaruh Interaksi Suami-Istri dan Interaksi Orang Tua- Anak terhadap Kesejahteraan Subjektif Keluarga di Kampung KBdan Non-KB.
Abstrak: Kesejahteraan subjektif keluarga merupakan suatu evaluasi kepuasan yang berkaitan dengan aspek fisik-ekonomi, sosial, dan psikologis. Penelitian ini bertujuan untuk menganalisis interaksi suami-istri, interaksi orang tua-anak, dan kesejahteraan subjektif keluarga di Kelurahan Pasirjaya. Penelitian ini menggunakan desain cross sectional melibatkan 120 keluarga utuh yang memiliki anak balita yang dipilih secara stratified disproportional random sampling menurut tempat tinggal (kampung KB dan kampung non-KB) di Kampung Muara, Kelurahan Pasirjaya, Bogor Barat. Hasil analisis menunjukkan terdapat korelasi positif antara kesejahteraan subjektif dengan interaksi suami-istri dan interaksi orang tua-anak. Hasil uji beda menunjukkan rataan lebih tinggi pada interaksi suami-istri (dimensi cinta, arahan, dan menghargai) dan interaksi orang tua-anak (dimensi arahan) pada keluarga di kampung KB. Hasil pada uji regresi menunjukkan bahwa kesejahteraan subjektif dipengaruhi secara positif oleh lama pernikahan dan interaksi orang tuaanak, namundipengaruhi secara negatif oleh besar keluarga.
Keyword: interaksi orang tua-anak, interaksi suami-istri, kampung KB, kampung non-KB, kesejahteraan subjektif

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable