Judul: Biosystematic Study of the Fern Genus Diplazium in West Malesia, Biosystematic Study of the Fern Genus Diplazium in West Malesia
Abstrak: Diplazium is a large genus consisting of about 400 species occur mainly in the tropics, sparingly in the subtropic and only locally extending into temperate. It was estimated that 300 species of the records were occurred in Malesia. Taxonomically, Diplazium is very difficult and quite insufficiently known. Therefore a comprehensive study on Diplazium in West Malesia was conducted by using morphological, ecological, geographic distribution, anatomical, palinological, cytological, as well as DNA analysis to understand the diversity and relationship among species. Based on gross morphological study on 1051 collection number of specimens as well as living collections, it was concluded that West Malesian Diplazium comprises of 69 species with 14 varieties. Thirteen species of them are proposed and described as new species, namely Diplazium asymmetricum, D. batuayauense, D. crameri, D. densisquamatum, D. halimunense, D. loerzingii, D. megasegmentum, D. megasimplicifolium, D. meijeri, D. parallelivenium, D. profluens, D. subalternisegmentum, and D. subvirescens. Two new varieties are poposed, namely D. accedens var. spinosum and D. silvaticum var. pinnae-ellipticum. D. pallidum var. montanum and D. accedens var. ridleyi are proposed as new status. Based on their main habitats, Diplazium can be classified into three major groups, viz. dryland (dominant), riparian and rheophytic species. Species diversity was culminated at 1000- 1500 m above sea level. The individuals with different genetic load in the same species sometimes grow in the different habitat gradients. Based on the range of the geographical distribution, West Malesian species can be divided into three types: (1) very wide species (19 species), (2) Malesian species (27 species), and (3) locally endemic species (23 species). Anatomical study on the transversal section of stipe of 27 species showed that the vascular bundle shape is varying among species. Therefore the leaf-trace shapes are important diagnostic features which support species delimitation in Diplazium. Spore morphology study showed that perine ornamentations support in delimitating species in Diplazium. However the phylogenetic analysis using parsimony revealed that morphological variation of spore is inadequate to depict natural relationship among Diplazium species. Cytological study on 117 collection number from 54 localities included in 31 species found that West Malesian Diplazium has six ploidy levels with x = 41 (diploid, triploid, tetraploid, pentaploid, hexaploid, and octoploid). New cytological information for science on 19 species are recorded. They are D. aequibasale (2n = 164), D. angustipinna (2n = 123). D. asymmetricum (2n = 123), D. batuayauense (2n = 164, 205), D. crenatoserratum (2n = 123, 164), D. halimunense (2n = 123), D. hewittii (2n = 123), D. profluens (2n = 164), D. loerzingii (2n = 82, 123), D. pallidum (2n = 82), D. petiolare (2n = 82), D. porphyrorachis (2n = 164), D. riparium (2n = 82, 123), D. spiniferum (2n = 82), D. subserratum (2n = 82, 123, 164), D. subvirescens (2n = 123), D. tomentosum (2n = 82, 205), D. xiphophyllum (2n = 82, 246), and D. wahauense (2n=164). Phylogenetic analysis on morphological data sets of 69 species using parsimony revealed that the phylogenetic relationship among species in the genus Diplazium was very difficult to explain due to the lack of or weak support
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Judul: Daemonorops fissa Complex in West Malesia
Abstrak: Daemonorops fissa complex is highly varied in West Malesia, but information about this complex is limited. The objective of the research was to reveal species diversity of Daemonorops fissa complex in West Malesia based on morphological and anatomical characters. Evaluation of morphological characters was conducted in Herbarium Bogoriense (BO) Cibinong Science Centre LIPI, Bogor (West Java). A total of 355 herbarium specimens were observed in this study representing all species of Daemonorops fissa complex. This study explored Daemonorops fissa complex, including the following: 1) species diversity, (2) distribution, (3) morphological variation, (4) anatomical variation and (5) phenetic relationships. Data obtained then was analized descriptively and phenetically. Species of Daemonorops fissa complex in West Malesia share some unique characters such as erect peduncle (to ± 2 cm), alternate leaves, length of involucrum 1 cm, globose fruits, and ruminate endosperm. This complex species consists of eight species namely, D. angustifolia, D. calicarpa, D. fissa, D. grandis, D. lewisiana, D. melanochaetes, D. monticola, D. stenophylla. Daemonorops binnendijkii, D.palembanica and D. trichroa proposed as new synonymies. Daemonorops binnendijkii and D. trichroa are new synonyms to D. angustifolia, and D. palembanica is a new synonym to D. melanochaetes. All species found in lowland rain forest, especially along stream and other wet places or more open areas, swampy area, ridgetop hill Dipterocarp forest, bamboo forest, and river side on altitude from 250 m – 1900 m asl. Morphological variation of Daemonorops fissa complex in West Malesia could be differentiated into 20 morphological characters. Cluster analysis separated all species into two main groups with coefficient similarity of 36%. Group I consisted of D. angustifolia, D. fissa, D. grandis, D. melanochaetes, D. monticola and D. stenophylla, while group II consisted of D. calicarpa and D. lewisiana. They were separated from five characters, total height, number of leaflets, width of leaflets, type of inflorescence and type of spine in leafsheath. Leaf anatomical characters of paradermal section could not be used to separate among species within D. fissa complex, but leaf anatomical characters of transverse section could be used to identify among species. All examined species of D. fissa complex have three similar characters, tetracytyc stomata, cryptopor type with sunken position and cell wall of sinous, but they have different pattern of bulliform cell and palisade tissues. Both characters are considered as two valuable new characters for supporting morphological data for species delimitation.
Keyword: anatomy, Daemonorops fissa complex, morphology, West Malesia

Judul: Desain Instrumen Pengontrol Otomatis Pasteurisasi Susu
Abstrak: Pasteurisasi merupakan salah satu cara pengawetan ter- hadap susu segar sebelum disimpan pada suhu rendah atau langsung dikonsumsi. Caranya adalah dengan memanaskan sam- pai mencapai suhu minimal 62.8 atau 71.7 °C yang diperta- hankan tetap selama 1800 atau 15 detik. Kombinasi suhu dan waktu dapat dipilih sesuai dengan kemampuan alat. Makin tinggi suhu yang dipilih makin pendek waktunya. Melalui proses pasteurisasi, rasa dan aroma susu tidak berubah dan seluruh bakteri penyebab penyakit (bakteri pa- togen) musnah. Berdasarkan kombinasi suhu-waktu, pasterisasi susu di- bedakan atas tiga metoda, yaitu metoda waktu lama (long hold), temperatur tinggi waktu pendek (HTST) dan temperatur tinggi (UHT). Suhu pasteurisasi dengan metoda waktu lama minimal 62.8 °C yang dipertahankan tetap selama paling se- dikit 1800 detik. Sedang HTST suhu minimalnya adalah 71.7 °C dengan waktu 15 detik atau lebih lama. Pada UHT waktu yang diperlukan adalah dua detik atau lebih singkat dengan suhu 87.8 - 132.2 °C. Pemakaian kombinasi suhu-waktu yang melebih dari ke- tentuan minimalnya akan mempengaruhi rasa, aroma, komposisi ...
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