Judul: Variasi Gen Pengkode Subunit E1β Branched-Chain α- Keto Acid Dehydrogenase Complex Pada Kambing Kacang Dan Kosta
Abstrak: Kompleks branched-chain α-keto acid dehydrogenase (BCKDH) merupakan kompleks multi enzim yang memiliki peran penting dalam regulasi katabolisme asam amino bercabang (branched-chain amino acids, BCAA). Informasi mengenai gen BCKDH telah dilaporkan pada sapi, sedangkan pada ruminansia kecil seperti kambing dan domba hingga saat ini belum ada laporan. Tujuan dari penelitian ini adalah memetakan variasi ekson 1, 3, dan 4 dari gen pengkode subunit E1β branched-chain α-ketoacid dehydrogenase complex (BCKDHB) pada kambing kacang (n=2) dan kosta (n=2) jantan. Proses amplifikasi DNA dengan teknik PCR menggunakan pasangan primer AF515-AF516, AF519-AF520, dan AF521-AF522 adalah sekuen ekson 1 (64 bp), ekson 3 (134 pb), dan ekson 4 (156 pb) yang conserved pada keempat sekuen yang dianalisis. Situs yang bervariasi terdapat pada daerah 5’UTR (C10G dan A11C), intron 1 (G199C, C302T, C461T, C538T, dan C583T), dan intron 4 (T553C dan A758G). Penyejajaran hasil deduksi asam amino menunjukkan bahwa keempat sekuen kambing identik dengan sekuen asam amino dari kambing hitam Yunnan yang telah dipublikasikan di GenBank (XM_005684454 dan NC_022301). Hal ini menunjukkan bahwa variasi nukleotida yang terdapat pada intron 1 dan 4 merupakan mutasi netral.
Keyword: BCAA, BCKDHB, kambing kacang, kambing kosta

Judul: Analisis Keragaman Nukleotida Ruas Ekson 4 Gen Branched Chain Ketoacid Dehydrogenase E1-α Polypeptide (BCKDHA) pada Sapi
Abstrak: Branched Chain Ketoacid Dehydrogenase (BCKD) is the enzyme complex that have a role in changing branched chain amino acid (leusin, isoleusin, and valin) into α-keto which can involve in Crebs cycle. Deficiency of enzyme complex BCKD will cause Maple Syrup Urine Disease (MSUD). This research aimed to analyze the variability of exon 4 nucleotides of BCKDHA gene on cattle. Analysis of variants from BCKDHA gene was performed using 5 DNA samples from madura cattle, 3 DNA samples from sumba ongole (so) cattle, and 3 DNA samples from ongole descendant cattle and nucleotides sequencing was conducted on PCR product from amplification of DNA pooling sample method. The variant of the exon 4 was G36T on po cattle. This variant revealed transversion substitution which changed Glu (GAA) to stop codon (TAA). The other variant, G144C was found in intron 4 of madura and so cattle.
Keyword: BCKDHA, exon 4, variant, DNA pooling, MSUD

Judul: Utilization of terra/MODIS L1B data for analysis of horizontal wind profile in the troposphere
Abstrak: This study was concerned with horizontal wind derived from data collected with the Moderate-resolution Imaging Spectroradiometer (MODIS) on board the NASA’s Earth Observation System (EOS)-Terra satellite. The aim of this study is to develop a method for the estimation of horizontal wind movement in the troposphere based on Navier-Stokes equation. The method was applied to a calibrated and geolocated Terra/MODIS L1B 1 km data set. The data inputs to estimate surface temperature are retrieved from TIR (Thermal Infrared) bands 31 (10.780 μm to 11.280 μm) and 32 (11.770 μm to 12.270 μm). The obtained surface temperatures were later used to approximate geopotential height on a constant-pressure surface by using the modified Poisson equation. The main assumptions used are the hydrostatic equilibrium, ideal gas law, constant lapse rate and constant acceleration of gravity. Based on the distribution of geopotential height, the pressure gradient force is measured by the gradient of geopotential on an isobaric coordinate to present the horizontal wind profile. For certain topographic areas especially in the mountain ranges, there was a disadvantage of using pressure as a vertical coordinate which lead to inaccuracy. In spite of that, Terra/MODIS L1B data with 1 km spatial resolution can be used to estimate the horizontal wind movement in the troposphere.
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