Judul: Pemetaan tingkat bahaya erosi sub das Bengawan Solo Hulu (Waduk Wonogiri
Abstrak: Tingkat Bahaya Erosi yang akan terjadi pada unit suatu lahan, dapat menentukan urutan prioritas Rencana Teknik Lapangan Rehabilitasi Lahan dan Konservasi Tanah (RTL RLKT). Tujuan dari penelitian ini adalah menduga - laju kehilangan tanah tiap grid, laju kehilangan tanah rata rata Sub DAS Bengawan Solo Hulu (Waduk Wonogiri), - Tingkat Bahaya Erosi rata rata Sub DAS Bengawan Solo - Hulu (Waduk Wonogiri), Menghitung prosentase masing masing Tingkat Erosi maupun Tingkat Bahaya Erosi, Menyajikan peta grid Laju Kehilangan tanah dan Tingkat Bahaya Erosi serta peta grid data penunjang. - Besarnya laju kehilangan tanah dihitung dengan metoda USLE sistem "grid method". Indek E130 diperoleh dari Peta Iso-eroden Bols. Erodibilitas tanah diperoleh dari Peta Tanah Tinjau. Informasi kemiringan lereng diperoleh dari Peta Lereng AGRARIA. Penggunaan lahan ditentukan dari Peta Penggunaan Tanah AGRARIA dengan 11 kategori penggunaan lahan. IPB Univ Kedalaman solum tanah berbeda beda untuk setiap - jenis tanah akibat proses pembentukan tanahnya...
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Judul: Aplikasi Sistem Informasi Geografis dalam Penentuan Tingkat Bahaya Erosi di Sub DAS Batanghari Hulu.
Abstrak: Watershed (DAS) Batanghari is a water catchment area which plays an important role in maintaining of ecosystem. Keputusan Kementerian Pekerjaan Umum (2012) states that forest degradation that occurs in the upstream Sub DAS. Prediction of erosion hazard was assessed using USLE with GIS software (ArcGIS 9.3 and ERDAS Imagine 9.1). The rate of erosion in the Sub DAS Batanghari Hulu calculated based on factors USLE erosion, i.e rain erosivity, soil erodibility, length and slope, land use and conservation actions. GIS analysis results showed that the rate of erosion in Sub DAS Batanghari Hulu is divided into five classes, i.e very light, mild, moderate, severe and very severeclass erosion. The Sub DAS Batanghari Hulu dominated by very mild class erosion, reaches 434 584.62 ha with a percentage of 34.80% of the total area. The Sub DAS Batanghari Hulu with very severe erosion hazard level has the smallest area, 64 430.04 ha, shows that most of the watershed area has a tolerable erosion value.
Keyword: erosion, gis, usle, watershed

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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