Judul: Genetic Diversity of Siamese Gourami from Sumatra, Java and Kalimantan for Selective Breeding of Fish Culture
Abstrak: Siamese gourami (Trichogaster pectoralis) is a species of fish that was introduced from Thailand to Indonesia in 1934. Siamese gourami farming activities during the period 2008-2011 ranged between 2.82-12.36% of total aquaculture production annually. Meanwhile, Siamese gourami fishing activities has led to overfishing and environmental degradation that could threaten its sustainability. The development of fish farming should be done for Siamese gourami supported by the efforts of domestication. Population genetics database of Siamese gourami from natural and domesticated populations stocks are necessary to be evaluated for its genetic structure and potential based on genotype fitness potential, selection, genetic drift, and population genetic diversity genotypes and phenotypes characteristics of intra and interpopulation are very important informations as references for sustainable management of genetic resources in aquaculture activities and selective breeding efforts through selection and crossbreeding. The purpose of this study is to identify the genotypes and phenotypes diversity of 9 Siamese gourami populations from Sumatra, Java and Kalimantan island based on Random Amplified Polymorphism DNA (RAPD) and truss morphometric analysis. The significancy of this research was to provide the population genetics database of Siamese gourami for selection and development of fish farming and breeding strategies on Siamese gourami culture. Fish samples of Siamese gourami analysed were originating from the island of Sumatra (Jambi, Palembang, Lampung), Java (West Java, Central Java, East Java), and Kalimantan (West Kalimantan, Central Kalimantan and South Kalimantan). Thirty samples of each population were used for truss morphometric analysis and 10 samples of fin specimen for RAPD analysis. DNA extraction was conducted using Phenol-Chloroform, while the primers of DNA amplification consisted of OPC 01-20 and 01-20 OPA. The DNA amplification process was held by polymerize chain reaction (PCR) using the reaction composition of a total volume of 25 mL: DNA 2 mL, 2 mL with a concentration of 10 pmol primer, 12.5 mL KAPA2G Robust HotStart Ready Mix, and 21 mL H2O. The characterization of truss morphometric were measured using with reference the theory of Blezinsky and Doyle method (1987). Water quality parameters in fish sampling locations included temperature, pH and dissolved oxygen.Three Siamese gourami populations showed genetic diversity above 50% which were as natural population collected from East Java (65.62%), Lampung (59.37%) and Palembang (56.25%). While two populations that had lower genetic diversity were collected from West Java (31.25%) and West Kalimantan (21.87%). As domestication population, the population showed a relatively low genetic diversity, these were from Central Java (12.5%) and Jambi (6.25%). Similarly with the population from Central Kalimantan (9.37%) and South Kalimantan (6.25%) that were from nature population. Based on genetic distance dendrogram of nine Siamese gourami populations from Sumatra, Java, and Kalimantan illustrated that the populations from West Java and South Kalimantan is the most distant, while three populations from East Java, Lampung and Palembang formed a different group with six other populations. By morphometric characteristics, the coefficient of variance 16 truss morphometric characters ranged between 2.92-12.99%, and the similarity index of intrapopulation was generally below to 50% except the population from Central Java showed high uniformity index (73.3%) and population from West Kalimantan (66.7%). Based
Keyword: RAPD, genetic diversity, truss morphometrics, Siamese gourami, Trichogaster pectoralis

Judul: Analisis Ragam Genotip RAPD dan Fenotip Truss Morfometrik Tiga Populasi Ikan Gabus Channa Striata (Bloch, 1793)
Abstrak: In order to manage genetic resouces for aquaculture development of snakehead fish, the evaluation of genetic variability of three populations from different geographical areas is needed to be performed. The purpose of this study is to identify the genotype and phenotype of snakehead fish from Jawa, Sumatera and Kalimantan using RAPD and truss morphometric. RAPD method used OPA-02, OPA-04 and OPA-07 primers was conducted to type the genotypes. Twenty one measurement of truss morphometric was done on the body of fish observed. The results showed that population from Java had highest percentage of polymorphism and heterozygosity than Sumatera and Kalimantan fish population that was, 83,33% and 0,3655. The population from Kalimantan and Jawa had the highest genetic distance was 0,1908. Population from Sumatera and Kalimantan had interpopulation relationship based on the similarity of truss morphometric was 50%. Coefficien of morphometric variation data showed that population of snakehead fish from Kalimantan was higher than Jawa and Sumatera.
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Judul: Pengembangan Desain Sistem Pakar untuk Diagnosa Kerusakan alat Berat Bulldozer D375A-2
Abstrak: Alat berat adalah sarana untuk mempercepat proses pelaksanaan suatu pekerjaan yang sukar bahkan tidak mungkin dilakukan oleh manusia. Dalam penggunaan alat berat seringkali mengalami kerusakan·. Kerusakan yang terjadi mengakibatkan tertundanya pekerjaan yang sedang dilaksanakan. Untuk memperbaiki kerusakan memerlukan biaya dan waktu yang cukup lama agar alat berat tersebut dapat berfungsi kembali.
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