Judul: Validasi Sekunder Metode Analisa Kapang dan Khamir pada Saos
Abstrak: Food are frequentlycontaminated by mold and yeast. The method used for analysis of mold on food in Indonesia set by BSN (Badan Standarisasi Nasional) was using potato dextrose agar (PDA) media. Problems are sometimes occured when using this media due to the spreading of mold colony that suppress other colonies to grow, resulting difficulties to asses morphology as well as enumeration of the mold. Statistifically, the outcome would be less acurate. Another method that could be used for the analysis of mold and yeast are the methods of ISO (International Standart Operation). Rose bengal dichloran chloramphenicol media (DRBC) was found to be a better media for quantative analysis of mold and yeast since it contains dichloran and rose bengal that could prevent the growth of spreading colonies. Laboratory experiments was needed to verify that using of different media can domonstrate the same performance for method enumeration. Secondary validation conducted for verification, if the laboratory using standard methods or adopting validated method. This Research was performed using the method of SNI 01-2897-1992 and ISO (International Standart Operation). Both of that methods were compared to obtained a valid methods. Parameters determined on method validation were accuracy precision, linearity, limit of detection, and limit of quantification. The results showed that all parameters were in accordance with the acceptance criteria of Internationa Standart Operation Method Validation. Accuracy was determined SNI method and ISO method with recovery percentage in range of 84%-109% for SNI and ISO method. The linearity in the concentration of 10; 100; 1000; and 10.000 cfu/gram resulted in correlation coefficent of 0,9979–0,9981. limit of detection in this method was 2cfu/gram and limit of quantification was 4 cfu/gram. For sauce product, the number of colonies grown in both media of DRBC and PDA were not significantly diffrent.
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Judul: Validasi dan verifikasi metode analisis bakteri asam laktat pada produk susu
Abstrak: Lactic acid bacteria (LAB) is a group of bacteria that capable converting carbohydrates (glucose) into lactic acid and mostly grow at facultatif anaerobic condition. At present, the most widely method used to enumerate LAB in some laboratory in Indonesia is incubation in aerob condition. In order to ensure that the method has a good performance, there is a need of method validation. Validation is the confirmation by examination and provision of objective evidence that particular requirements for specified intended use are fulfilled. The purpose of this study is to validate the quantitative method for enumeration of lactic acid bacteria. Two methods of enumeration of LAB in yoghurt were studied, i.e. ISO 7889-2003(E) as a reference method and SNI 2981-2009 as an alternative method. The principle of this method is to grow the facultative anaerobic bacteria in aerobic and anaerobic condition incubated for 3 days at 37 oC on MRSA media. The anaerobic condition was made using Anaxomat jar whose enrich with 10% CO2. The result obtained by these two method were compared. Therefore several parameters such as precision (RSD), relative accuracy, linearity, limit of detection, and limit of quantification were evaluated. The results showed that RSD of these methods between 0.44% and 3.03%, percent recovery of these methods between 97% and 101%, and limit of detection are 3 cfu/mL. It can be concluded that these two methods have good performance characteristics.
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Judul: Formulasi Hamiltonian untuk Menggambarkan Gerak Gelombang Soliter Dimensi Tiga di Permukaan Laut
Abstrak: Gelombang permukaan adalah gelombang yang terjadi perbedaan rapat massa antara air dan udara. Salah satu gelombang permukaan yang ditinjau adalah gelombang soliter. Formulasi gelombang soliter dilakukan dengan menggunakan formulasi Hamiltonian.
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