Judul: Composition of tree species and standing structure of mangrove forest in the pasar banggi village rembang central java province
Abstrak: Ekosistem mangrove Desa Pasar Banggi merupakan salah satu yang cukup baik di Pantura Jawa Tengah. Jenis pohon yang dapat ditemui di hutan mangrove ini yaitu Rhizophora mucronata, Rhizophora apiculata, Sonneratia alba, danAvicennia marina. Pada tingkat semai didominasi oleh Rhizophora mucronata dengan nilai INP 175.99 begitu pula dengan tingkat pancang dan pohon juga didominasi oleh jenis Rhizophora mucronata dengan nilai INP masing-masing 208.2 dan 183.19. Indeks keragaman (H’) pada hutan mangrove ini tergolong rendah, tingkat semai memiliki indeks keragaman dengan nilai hanya 0.95 tidak jauh berbeda dengan tingkat pancang dan pohon yang masing-masing 0.84 dan 0.75. Tingkat kerawanan degradasi dari parameter kerapatan vegetasi masuk ke dalam tingkat rendah, sedangkan dari parameter indeks keragaman tergolong tingkat tinggi yang menunjukan rawan degradasi. Struktur tegakan secara umum berbentuk huruf “J” terbalik kecuali jenis Sonneratia alba, hal ini kemungkinan disebabkan kegagalan regenerasi oleh jenis Sonneratia alba., Mangrove ecosystem at the Pasar Banggi village is one of the mangrove ecosystem which is quite good in the northern coastal of Central Java. The tree spesies that can be found in this mangrove forest is Rhizophora mucronata, Rhizophora apiculata, Sonneratia alba and Avicennia marina. For seedlings dominated by the Rhizophora mucronata with score of Important Value Indeks 175.99 similarly with saplings and trees are also dominated by Rhizophora mucronata types with Important Value Indeks 208.2 and 183.19. Index of biodiversity in mangrove forests is relatively low, seedlings have a diversity index with a value of only 0.94 is not much different from the saplings and trees which are also only 0.84 and 0.75. The vulnerability of the degradation for density parameters into the low class while the parameters of the biodiversity index into high classified which showed cartilage degradation. Stand structure in general shape of reverse "J" exception types Sonneratia alba, this condition maybe caused by unsuccsed regeneration for Sonneratia alba.
Keyword: ekosistem mangrove, komposisi jenis, struktur tegakan

Judul: Cadangan Karbon Tegakan Mangrove di Desa Pasar Banggi Kabupaten Rembang, Jawa Tengah
Abstrak: Mangroves are usually used as a prevention of coastal erosion and the fish farming (ponds). Ecologically mangrove has potential as carbon stocks. Mangroves that located in Pasar Banggi Village Rembang Regencyhave a function as conservation area. There are four types of mangroves such as Rhizophora mucronata, Rhizophora apiculata, Sonneratia alba, and Avicennia marina. The purposes of this study are to calculate the biomass,the carbon stocks and CO2 sequestration estimation using per tree method and midpoint diameter calculation method. The result showed that the value of the biomass, carbon stocks, and CO2 sequestration consecutively are 81.274 ton/ha, 38.199 ton/ha, dan 140.190 ton/ha. The use of midpoint diameter calculation method can not be applied to find out the value of the biomass, carbon stocks, and CO2 sequestration in mangrove of Pasar Banggi Village Rembang Regency because it has a significant difference.
Keyword: Bogor Agricultural University (IPB), estimation, CO2 sequestration, carbon stocks, biomass, mangrove

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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