Judul: Distribusi Sedimen Pantai di Ujung Genteng, Sukabumi, Jawa Barat
Abstrak: Distribusi sedimen pantai Ujung Genteng dipengaruhi oleh karakteristik hidrooseanografi Samudera Hindia. Penelitian ini bertujuan mempelajari sebaran jenis dan ukuran butir sedimen pantai dan kaitannya dengan kemiringan pantai dan dinamika perairan Samudera Hindia. Sampel sedimen diambil di enam titik pengamatan dan dianalisis dengan menggunakan metode pengayakan kering. Data hidrooseanografi di wilayah Ujung Genteng dianalisis secara spasial dan temporal, dan data kemiringan pantai dianalisis secara spasial. Pada Musim Barat, tinggi gelombang berkisar 0.99-2.95 m dengan kecepatan arus 0.10-127.60 cm s-1 dan curah hujan 321-590 mm/bulan, sedangkan pada Musim Timur tinggi gelombang berkisar 0.96-3.74 m dengan kecepatan arus berkisar 0.14-104.87 cm s-1 dan curah hujan 0-59 mm/bulan. Tipe kemiringan pantai di seluruh stasiun termasuk kategori pantai datar. Sedimen di Ujung Genteng didominasi oleh pasir sangat kasar dan pasir sedang dimana semakin kearah selatan ukuran butir sedimen semakin besar. Tinggi gelombang dan kecepatan arus yang lebih besar di bagian selatan menyebabkan ukuran butir sedimen yang lebih kasar dan kemiringan pantai yang lebih besar dari pada di bagian utara.
Keyword: sedimen, kemiringan pantai, Ujung Genteng, arus, gelombang

Judul: Distribusi Sedimen di Sekitar Muara Sungai Cimandiri Teluk Palabuhanratu Sukabumi.
Abstrak: Muara Sungai Cimandiri yang terletak di kepala Teluk Palabuhanratu memberikan masukan sedimen yang cukup besar ke perairan teluk. Penelitian ini bertujuan mempelajari dinamika perairan dan pengaruhnya terhadap sebaran ukuran butir sedimen. Penelitian ini juga memetakan sebaran sedimen di sepanjang pesisir muara Sungai Cimandiri. Sedimen dianalisis dengan fraksinasi dan metode pipet. Data pasang surut, curah hujan, dan debit sungai dianalisis secara time series sedangkan gelombang menggunakan MIKE 21 Spectral Wave FM. Titik pengambilan sampel dilakukan di sepanjang pesisir utara Muara Sungai Cimandiri hingga ke bagian selatan. Pada Musim Barat curah hujan dapat mencapai 726 mm dengan debit sungai sebesar 668.82 m3/s dan menghasilkan masukan sedimen sebesar 189.6 kg/s selanjutnya kecepatan arus di Musim Barat sebesar 11.6-21.7 cm/s dengan tinggi gelombang mencapai 1.05 m, sedangkan pada Musim Timur arus mampu mencapai 13.0-16.1 cm/s, tinggi gelombang 0.9 m, curah hujan 129 mm, dan debit sungai sebesar 376.87 m3/s sehingga masukan sedimen ke perairan hanya 10.62 kg/s. Sedimen yang berada di wilayah penelitian menunjukkan fraksi butir sangat halus, fraksi sedimen pada bagian utara sedikit kasar dibandingkan bagian selatan. Pengaruh jetty menghalangi sebaran sedimen ke utara sedangkan pengaruh penambangan pasir membuat ukuran sedimen di muara sungai lebih halus karena fraksi sedimen kasar diambil untuk penambangan pasir.
Keyword: fraksinasi, sedimen, metode pipet, muara Sungai Cimandiri

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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