Judul: Keberadaan Antibodi Antiprotease Escherichia Coli Enteropatogenik Pada Neonatus dan Balita Penderita Diare
Abstrak: Pengambilan serum dilakukan pada 5 orang balita penderita diare dan 30 orang neonatus. Sebagai antigen digunakan protease EPEC K.l.l yang merupakan protein ekstraselular. Antigen diperoleh dengan menumbuhkan bakteri pada media minimaI M, dan isolasi protein dilakukan dengan amonium sulfat 45%. Keberadaan antibodi antiprotease EPFlC pada serum penderita diare dan neonatus dilihat dengan uji Dot Blot. Sebagai kontrol positif, digunakan serum meneit Balb/c yang telah diimunisasi dengan antigen protease EPEC. Produksi serum tersebut dilakukan dengan imunisasi protease EPEC ditambah adjuvant dilakukan sebanyak lima kali, dengan dosis 25 ~g'"per ekor, rute subkutan, interval 8-10 hari. Dari penelitian ini diperoleh hasil pada 40% serum balita penderita diare dan 13,3% serum neonatus terdapat antibodi antiprotease EPEC. Semua serum neonatus dari ibu yang sehat, pada uji Dot Blot menunjukkan hasil negatif. Pada serum neonatus dari ibu yang tidak pernah menderita diare namun pernah menderita penyakit lain, didapatkan tiga sampel positif. Hal ini menunjukkan adanya kemungkinan antigen protease EPEC K. 1.1 dapat bereaksi silang dengan antigen penyebab penyakit lain.
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Judul: Deteksi Keberadaan Antibodi Anti-Escherichia coli di Dalam Serum Sapi Neonatus yang Diberi Kolostrum dengan Metode Enzyme-Linked Immunosorbent Assay (ELISA)
Abstrak: The aim of this research is to study the present of antibody against E. coli in the sera of neonates calf which given colostrum and challenged by enterotoxigenic Escherichia coli (ETEC) K99. Eleven neonates calves were clinically healthy kept since birth until the age of one week.This neonates calves are divided into two groups, first group is colostrums group (8 calves) and the other is non-colostrum group (3 calves). Colostrum group were given colostrum on age 0-72 hours with 12 hours interval, whereas non-colostrum group are given cow milk at the same time. Each group were challenge by ETEC K99 5X1010 CFU emulsion it in alhydrogel peroral at the 12 hours of age. Blood sample were taken from jugularis vein on age 0th, 12th, 24th, 48th, 72th, and 168th hours. The present of antibody in the sera detecting by indirect ELISA (Enzyme-Linked Immunosorbent Assay Method). The result showed of the highest concentration of antibody in the sera at 24 hours of age for colostrums group, whereas no antibody against E. coli were detected for non-colostrums group . Based on the results, we concluded the concentration of antibody in colostrum can detection at 24 hours until one week after given colostrum. The highest concentration of antibody anti- E. coli in 24 hours after given colostrum.
Keyword: colostrum, neonates calf, antibody anti-E. coli, sapi neonatus, Kolostrum,

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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