Judul: Pengaruh pengetahuan, motivasi, dan akses Informasi terhadap niat komplain pada pengguna listrik
Abstrak: Adanya ketidakpuasan terhadap pelayanan listrik akan memunculkan suatu niat kompain. Penelitian ini bertujuan untuk menganalisis pengaruh pengetahuan, motivasi, dan akses informasi terhadap niat komplain. Desain penelitian yang digunakan adalah cross sectional study dengan lokasi penelitian di Kelurahan Ciparigi, Kecamatan Bogor Utara, Kota Bogor. Contoh dalam penelitian ini sebanyak 120 suami atau istri yang mengambil keputusan dalam melakukan komplain ketika mengalami permasalahan tentang listrik dan yang sering berinteraksi dengan karyawan PLN atau PT PLN. Pengambilan data dilakukan dengan wawancara menggunakan kuesioner. Hasil penelitian menunjukkan bahwa motivasi komplain, jumlah informasi tentang komplain, dan status pekerjaan secara signifikan berpengaruh positif terhadap niat komplain. Faktor yang tidak berpengaruh terhadap niat komplain antara lain pengetahuan hak dan kewajiban konsumen dan komplain, jumlah sumber informasi tentang hak dan kewajiban konsumen, usia, jenis kelamin, lama pendidikan, dan pendapatan., The dissatisfaction toward electricity will triger complaint intention. This research was aimed to analyze effects of knowledge, motivation, and information accsess on complaint intention. This study used cross sectional design that held in Sub-district Ciparigi, District North Bogor, Bogor City. Samples involved in this research as many as 120 persons whether husband or wife who takes decision on complaint related to electricity and frequently interacting with PLN officer or PT PLN. The data was obtained by using questionnaired interview. The result showed that complaint motivation, the number of information accsess related to complaint and occupational status were positively significant influence on complaint intention. There were several factors did not influence on complaint intention, such as knowledge of consumer right and obligation also complaint, number of information resources related to consumer right and obligation, age, sex, education length, and income.
Keyword: niat komplain, pengetahuan, motivasi, akses informasi, dan kewajiban konsumen

Judul: Pengaruh pengetahuan dan kesadaran terhadap niat Komplain pada barang dan jasa
Abstrak: Niat komplain merupakan tindakan berupa keluhan yang akan dilakukan oleh konsumen ketika barang dan jasa yang digunakan tidak sesuai dengan harapan. Keluhan yang akan disampaikan oleh konsumen sangat ditentukan oleh pengetahuan dan kesadaran akan hak dan kewajiban konsumen yang dimiliki. Tujuan penelitian ini adalah menganalisis pengaruh pengetahuan dan kesadaran terhadap niat komplain pada barang dan jasa. Disain penelitian ini menggunakan cross sectional study dengan metode survei yang dilakukan di Kelurahan Paledang, Kecamatan Bogor Tengah, Kota Bogor. Penelitian ini melibatkan 90 contoh, yang dipilih dengan menggunakan teknik purposif. Hasil penelitian menunjukkan hanya tiga jenis barang yang bermasalah setelah dibeli yaitu, gadget, fashion (pakaian, kosmetik, sepatu atau sandal), dan kendaran (mobil, motor, dan sepeda) memiliki hubungan yang signifikan untuk responden berniat melakukan komplain. Tingkat pengetahuan objektif dan kesadaran hak dan kewajiban tergolong tinggi sedangkan pengetahuan objektif tentang komplain, pengetahuan subjektif hak dan kewajiban serta komplain tergolong sedang. Hasil penelitian menunjukkan pengetahuan tidak memengaruhi niat komplain, tetapi kesadaran memengaruhi niat komplain. Selain itu, faktor yang memengaruhi niat komplain pada barang dan jasa adalah akses infomasi, dan ketidakpuasan konsumen., Complaint intentions is the action in the form of complaint that will be carried out by the consumer when the goods and services that are used are not in line with expectations. Complaints that will be submitted by the consumers is determined by the knowledge and awareness of the rights and obligations of consumers owned. The purpose of this research was to analyze the influence of knowledge and awareness toward complaint intentions on goods and services. The design of the research was used a cross-sectional study with a survey methods on Paledang Village, District of Central Bogor, Bogor. The research involved 90 samples, which were selected by using purposive technique. The results showed that only three types of problemtic goods after purchasing (gadgets, fashion (clothes, cosmetics, shoes or sandals), and the vehicle) have a significant relationship with complaint intentions of respondent. The level of objective knowledge and awareness of rights and obligations are classified high, whereas the objective knowledge about the complaints, subjective knowledge about the rights, obligations and complaints are classified moderate. The results showed that knowledge didn’t affect the intention of complain, but awareness affected the intention of complain. Moreover, factors that influenced the complaint intentions on goods and services were the amount of information access, and consumer’s dissatisfaction.
Keyword: hak dan kewajiban konsumen, kesadaran, niat komplain, dan pengetahuan

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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