Judul: Identifikasi Senyawa Aktif Lengkuas (Alpinia galanga) Dengan LC-MS dan Potensinya Sebagai Inhibitor Lipase Pankreas Secara In Silico
Abstrak: Prevalensi penderita obesitas yang terus meningkat setiap tahunnya, serta risiko komplikasi yang ditimbulkan membuat perhatian besar saat ini. Orlistat berefek buruk karena sifatnya yang bekerja dengan menginhibisi enzim lipase pankreas secara ireversibel. Lengkuas banyak ditemukan di Indonesia diketahui dapat berperan sebagai antiobesitas. Penelitian ini bertujuan mengidentifikasi senyawa aktif lengkuas yang berpotensi memiliki aktivitas inhibisi enzim lipase pankreas dengan menggunakan YASARA. Senyawa aktif lengkuas diekstrak menggunakan etanol 70% dan diidentifikasi menggunakan LC-MS/MS QToF serta dilakukan studi pustaka. Sebanyak 8 senyawa berhasil diidentifikasi menggunakan LC-MS/MS QToF dan termasuk golongan polifenol, fenolik, terpen, dan flavonoid, serta terdapat 38 senyawa yang hasil studi pustaka. Terdapat 4 senyawa aktif lengkuas memiliki potensi antiobesitas dengan empat yang terbaik yaitu, galangin, galaganol C, isorhamnetin, dan kampferide. Galangin menjadi senyawa yang paling potensial menjadi kandidat antiobesitas pada tanaman lengkuas., The prevalence of obesity, which continues to increase every year, as well as the risk of complications caused by it, is of great concern today. Orlistat has adverse effects because it works by irreversibly inhibiting the pancreatic lipase. Galangal, found in Indonesia, is known to act as an antiobesity agent. This study aimed to identify active compounds of galangal that have the potential to inhibit pancreatic lipase using YASARA. The active compounds of galangal were extracted using 70% ethanol and identified using LC-MS/MS QToF and literature study. A total of 8 compounds were successfully identified using LC-MS/MS QToF and included polyphenols, phenolics, terpenes, and flavonoids, and there were 38 compounds from literature studies. There are 4 active compounds that have antiobesity potential with the four best ones, namely, galangin, galaganol C, isorhamnetin, and kaempferol. Galangin is the most potential compound to be an antiobesity candidate in galangal plants.
Keyword: Alpinia galanga, pancreatic lipase, antiobesity, molecular docking

Judul: Potensi Senyawa Aktif Serai Wangi (Cymbopogon nardus) sebagai Antiobesitas melalui Penghambatan Lipase Pankreas  In Silico
Abstrak: Obesitas telah menjadi permasalahan gizi yang mendunia. Kejadian ini ditandai oleh IMT ≥ 30 kg/m2. Kandungan β-sitronelal dan β-sitronelol dalam serai wangi pada penelitian in vivo terbukti dapat menurunkan berat badan melalui peningkatan aktivitas saraf simpatik yang berdampak pada penurunan nafsu makan, namun potensinya sebagai inhibitor lipase pankreas in silico belum diketahui. Penelitian ini bertujuan menguji potensi senyawa aktif serai wangi sebagai inhibitor HPL 1LPB in silico. Metode yang digunakan yaitu preparasi protein dan ligan, validasi gridbox, penapisan virtual, visualisasi interaksi residu asam amino, serta analisis bioavailabilitas dan toksisitas. Hasil penelitian menunjukan bahwa sebanyak 26 dari 47 ligan uji hasil studi literatur dan analisis LCMS diketahui memiliki kemampuan yang baik dalam menghambat lipase pankreas (Kode: 1LPB) dibandingkan dengan MUP berdasarkan parameter energi ∆G, Kd, dan %BSS. Ligan uji apigenin-8-C-glucoside yang termasuk ke dalam golongan senyawa fenolik merupakan ligan yang memiliki penghambatan paling kuat dengan nilai ∆G -8,326 kkal/mol, Kd 0,78 µM dan %BSS sebesar 93% serta memenuhi analisis bioavailabilitas dan toksisitas. Oleh karena itu serai wangi diprediksi memiliki potensi sebagai antiobesitas melalui penghambatan lipase pankreas dan aman apabila dikembangkan menjadi kandidat obat., Obesity has been one of nutrient problems in the world. This is indicated by IMT ≥ 30 kg/m2. The component of β-citronellal and β-citronellol in serai wangi, based on this research in vivo discovered that it can reduced the weight through incresing sympathic neuron causing the reducing of apatite, yet its potency as inhibitor as pancreatic lipase in silico was not proved. The research is intended for testing the potency of active compounds of Serai Wangi as inhibitor of HPL 1PLB in silico. The method applied was protein and ligan preparation, gridbox validation, virtual screening, amino acid visualization interaction, with bioavailability and toxicity analysis. The research found that the number of 26 from 47 Ligan as the result of literature study and LCMS analysis was discovered having good ability in inhabiting pancreatic lipase (code: 1LPB) compared with MUP based on ∆G, Kd, dan %BSS parameters. Ligand of apigenin-8-C glucoside belongs to phenolic compounds as the most potential ligan in inhabiting pancreatic lipase with the value ∆G -8,326 kcal/mol, Kd 0,78 μM and % BSS up to 93% and fulfilling bioavailability and toxicity analysis. That is why serai wangi was predicted having the potency of antiobesity through inhabiting pancreatic lipase and safe for candidate medicine.
Keyword: Obesity, pancreatic lipase, serai wangi, in silico

Judul: Implementation of Spatio-Temporal Change Query in Relational Database.
Abstrak: Geographic object changes in various views. Object changes are recorded using a model of static storage. No historical connection between old version data and new version data has been built. For that, a storage model of spatio-temporal queries that can analyze spatio-temporal changes has been developed. The object changes the geometry are grouped according to the taxonomy of Spatio-Temporal Pattern Change. This research has been able to apply the query patterns change including born, expand, merge, contract, split, normal and die. This query based on geometric (area) changes. The implementation of spatio-temporal change queries represents an object changes continuously with easy, change detection is done by comparing two versions of data. Keyword: spatio-temporal change query, spatio-temporal change pattern.
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