Judul: Pola Distribusi Geografis Pada Udang Mantis Di Laut Jawa Berdasarkan Genom Mitokondria
Abstrak: Mantis shrimp or ronggeng shrimp (Harpiosquilla harpax) is a large sized shrimp. The mostly habitat of mantis shrimp is beach. Shrimps are exploited for trade as an exotic food. Shrimp liked as a source of food because large size and presentation of meat can be as high as 50% by weight. Characteristics stomatopod as predators have two methods to catch prey are smashers and spearers. H. Harpax classified into spearers. This research was conducted to analyze the genetic diversity of mantis shrimp H. harpax in Java Sea and study the distribution based on mitochondrial genome. The sample used is the mantis shrimp H. harpax many as eight tails collection of Dr. Yusli Wardiatno from the Department of Aquatic Resources and Management IPB preserved in ethanol. Amplification was tested in vitro with the technique of Polymerase Chain Reaction (PCR). Product amplification is 1100 bp were arranged and phylogeni analyzed. All of the eight sample are successfully amplified. The distribution pattern of mantis shrimp larvae is mainly larvae carried of alongshore current shoreline. According by filogeografi H. harpax in Cirebon water separately with H. harpax in Vietnam. This is evidenced by the value of the farthest genetic distance is between H. harpax Vietnam and H. harpax 3 is 10.5%, while the closest genetic distance was between H. harpax 1 and 6 (group 1) is 1.8%. Genetic Diversity of H.harpax in Cirebon Aquatic based on d-loop segment has a greater value is 5.1% (± 0.005).
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Judul: DNA Barcode as an Alternative of Mantis Shrimp Species Identification
Abstrak: Udang mantis atau yang dikenal dengan udang ronggeng merupakan salah satu anggota Subfilum Crustacea Ordo Stomatopoda, Beberapa spesies udang mantis dikenal sebagai komoditi ekspor dan makanan eksotis. Beberapa udang mantis yang bernilai ekonomi tinggi adalah anggota famili Harpiosquillidae dan Squillidae. Udang-udang mantis tersebut biasa ditangkap dari Laut Jawa dan Laut Cina Selatan. Penelitian ini bertujuan untuk mengaplikasikan teknik DNA barcoding sebagai laternatif mengidentifikasi udang mantis sampai ke tingkat spesies. Sebanyak 34 sampel udang mantis berasal dari Jambi, Cirebon, dan Aceh yang digunakan adalah spesimen koleksi yang diawetkan dalam alkohol. Identifikasi spesies dilakukan berdasarkan morfologi. Ekstraksi DNA dilakukan dari otot tungkai dan ruas gen CO1 diamplifikasi sebagai ruas DNA yang dijadikan barcode. Amplifikasi gen CO1 berhasil dilakukan untuk semua sampel yang kesemuanya menghasilkan amplikon yang multiband. Perunutan nukelotida hanya berhasil dilakukan untuk tujuh sampel, yaitu Harpiosquilla harpax (Sampel No. 34 dan No. 37 asal Jambi), Harpiosquilla stephensoni (sampel No. 1 dan No. 2 asal Cirebon), dan Carinosquilla multicarinata (sampel No. 9 dan No. 11 asal Cirebon, dan No. 22 asal Aceh). Berdasarkan ruas gen CO1, C. multicarinata asal Aceh memiliki kekerabatan yang berbeda dengan C. multicarinata asal Cirebon.
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Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4