Judul: Keterandalan dan Intensitas Keruskan Konstruksi Bangunan Gedung Permukiman Di Jakarta Selatan
Abstrak: Jumlah penduduk berkaitan erat dengan peruntukan lahan bagi bangunan pemukiman. Proses kerusakan bangunan salah satunya disebabkan oleh faktor manusia. Manusia perlu pengetahuan tentang kondisi bangunan, faktor-faktor perusaknya dan cara perawatan sedini mungkin sehingga masa pakai bangunan bisa menjadi lebih lama. Penelitian ini bertujuan untuk mengetahui keterandalan dan intensitas kerusakan bangunan gedung permukiman di Jakarta Selatan. Penentuan bangunan rumah contoh dilakukan dengan teknik pengambilan contoh acak dengan empat tahap. Pada masing-masing rumah dilakukan pengamatan terhadap ada atau tidaknya kerusakan pada komponen bangunan rumah serta dilakukan wawancara dengan masing-masing penghuni rumah untuk menghimpun informasi umur bangunan, pemeliharaan dan perawatan bangunan yang kemudian dicatat pada kuesioner. Data yang diperoleh, kemudian dihitung keterandalan gedung dan intensitas kerusakan bangunan gedung. Hasil penelitian menunjukan bahwa keterandalan bangunan gedung permukiman di empat perumahan di Jakarta Selatan sebagian besar berkategori sedang. Sementara itu intensitas kerusakannya berkisar antara 34.65%sampai dengan 44.8%.
Keyword: Construction, Demage intensity, Structure, Model houses, Building could, Intensity, Serangan rayap, Kayu lapuk

Judul: Frekuensi dan Intensitas Serangan Rayap Tanah pada Bangunan Gedung milik Pemerintah Provinsi DKI Jakarta.
Abstrak: Jakarta is a province with the most developed infrastructure in Indonesia, including its building constructions. However, termites attack were often found on the buildings in this region. This study was conducted to investigate the frequency and intensity of termites attack on Jakarta Government’s buildings. The targeted buildings in this research were selected by four stage stratified random sampling technique. Termites speciemens were collected from attacked buildings, then identified according to (Ahmad 1958). The results of this research showed that the frequency of termites attack on Jakarta Government’s buildings reaches 16.67% with the degree of infestation could classified as light infestation. Compared to other building components, windows sills has the highest termites attack rate (14.58%), followed by door sills (8.34%), and roof truss (4.16%). In addition, Microtermes inspiratus Kemner (Isoptera: Termitidae) have been found as the most frequent termite species attacking the buildings in Jakarta Province with infestation frequency reaches 77.8%, followed by Coptotermes curvignathus Holmgren (Isoptera: Rhinotermitidae) with infestation frequency of 22.22%. The highest intensity of termites attack occurred in Cililitan Village, Subdistrict Kramat Jati, East Jakarta.
Keyword: buildings, Jakarta, termites

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable