Judul: Analisis Proksimat dan Profil Asam Amino pada Maggot Black Soldier Fly Setelah Perlakuan Tripsin
Abstrak: Maggot black soldier fly (Hermetia illucens) merupakan salah satu komuditas serangga yang populasinya berlimpah di alam dengan kandungan protein dan lemak yang tinggi. Penelitian ini bertujuan mengidentifikasi kandungan asam amino yang terdapat di dalam maggot bsf yang berasal dari Depok, dengan metode High Performance Liquid Chromatography (HPLC). Analisis yang dilakukan pada penelitian ini yaitu uji proksimat, ekstraksi dan uji digesti protein dengan menggunakan metode Bradford dan ninhidrin, serta uji Tukey menggunakan analisis ANOVA. Hasil penelitian menunjukkan bahwa ekstraksi protein pada maggot bsf berhasil dilakukan, dan didapatkan kadar protein sebelum dan setelah dihidrolisis dengan tripsin yaitu 224,74 μg/mL dan 102,52 μg/mL dengan selisih yang berbeda nyata (P<0.05), yang menunjukkan bahwa hampir setengah dari total protein maggot dapat dicerna oleh tripsin. Jenis asam amino yang digunakan sebagai suplemen untuk atlet atau olahragawan yaitu valin, isoleusin, dan leusin, dengan konsentrasi masing-masing sebesar 40, 40 dan 50 μg/mL, yang diperoleh dari filtrat hasil ekstraksi protein. Konsentrasi asam amino dari filtrat hasil ekstraksi protein yaitu sebesar 1218,64 μg/mL, lebih tinggi daripada filtrat hasil digesti protein yaitu hanya 290,75 μg/mL.
Keyword: asam amino, HPLC, maggot bsf, protein, tripsin

Judul: The Performance of Maggot Black Soldier Fly (Hermetia  illucens) on Different Carbohydrate Protein Ratio Substrates
Abstrak: Penelitian ini bertujuan mengevaluasi maggot yang diberi pakan dengan rasio karbohidrat protein yang berbeda. Penelitian ini menggunakan 2 perlakuan dan 5 ulangan, perlakuan substrat dengan rasio karbohidrat : protein 88%:12% (P1) dan 74%:26% (P2), substrat perlakuan diberikan sebanyak 2,25 kilogram selama pemeliharaan. Parameter performa yang diukur yaitu konsumsi bahan kering (BK), protein kasar (PK), lemak kasar (LK), serat kasar (SK) dan asam amino, bobot hidup, persentase prepupa, reduksi substrat, serta feed conversion ratio (FCR). Data dianalisis menggunakan sidik ragam T-Test (Independent Sample T-Test). Performa maggot BSF perlakuan P2 menunjukkan hasil yang nyata lebih tinggi (P<0,05) dibandingkan dengan perlakuan P1 pada parameter konsumsi BK dan PK, bobot hidup, persentase prepupa, reduksi substrat, serta FCR. Konsumsi asam amino esensial tertinggi yaitu leusin, lisin, dan threonin, sementara konsumsi asam amino nonesensial tertinggi yaitu asam glutamat, asam aspartat, dan glisin. Kesimpulannya, pemberian pakan rasio protein tinggi (74%:26%) menghasilkan performa paling baik dan optimal., This study aimed to evaluate maggots' performance that fed with different protein-carbohydrate ratios. This study used two treatments and five replications, substrate with ratio of carbohydrate and protein 88%:12% (P1) and 74%:26% (P2), 2,25 kilogram substrates was given to the maggot during cultivation. The performance parameters measured were consumption of dry matter (DM), crude protein (CP), ether exctract (EE), crude fiber and amino acids, maggot weight, percentage of prepupae, substrate reduction, and feed conversion rasio (FCR). Data were analyzed using a T-Test of variance (Independent Sample T-Test). The performance of BSF larvae on P2 treatment were significantly higher (P<0.05) compared to the P1 treatment from consumption of DM and CP, maggot weight, percentage of prepupae, substrate reduction, and FCR. The highest consumption of the essential amino acids were lysine, isoleucine, and arginine, while the highest consumption of the non-essential amino acids were glutamic acid, aspartic acid, and alanine. In conclusion, maggot fed with high protein ratio (74%:26%) produced the best and most efficient performance.
Keyword: asam amino, black soldier fly, performa, rasio karbohidrat protein

Judul: Thermal Characteristics and Dynamics of Tropical Cyclone over Western North Pacific Ocean: A Case Study of Typhoon Songda (Chedeng) 2011
Abstrak: The frequent occasion of Tropical Cyclone (TC) over Western North Pacific (WNP) Ocean lies from 10˚N to 26˚N and 121˚E to 170˚E. Sources of TC development come from warm ocean temperature where latent heat is released during condensation processes and subsequently concentrated in the boundary layer. Relative humidity at 850-700 hPa is responsible to produce the most intense storm. Meanwhile, weak vertical wind shear between 850-200 hPa is one of the dynamical factors that lead to the development of TC. Pressure level of 850-200 hPa is chosen to minimize the thermodynamic effect in the lower troposphere. Typhoon Songda (Chedeng in the Philippines) or TSC lasted from May 18th to 30th, 2011 and strengthened into powerful Super Typhoon stage between May 24th and 27th. Long curving track and the exhibition of all sequences of TC life cycle, TSC is then considered as an ideal case of TC. The objective of the study with case of TSC is to investigate the role of thermal characteristics and dynamics on TC intensity. The research is carried out by using Advanced Research of Weather Research and Forecasting model (WRF ARW model) version 3.3. Two domains are employed in the model. The first domain situate from 02˚N to 40˚N and 118˚E to 149˚E and cover spatial resolution () of 33.1 km. The second domain situate from 09˚N to 27˚N and 119˚E to 132˚E with of 11 km. Timestep of the model () is 120 seconds. The model is supported by the following physical parameterization, i.e., Yonsei University Scheme (YSU) in the boundary layer, Kain-Fritsch scheme (KF) in the cumulus, and WRF Single-Moment 3-class scheme in the microphysics. Initial meteorological condition prior to the formation of the cyclone is generated using WRF ARW standard-release model. Sensitivity of the model output is obtained from a selected single-parameter change scenarios, namely, 10 % to 20 % decrease and increase of wind shear initial data at 850-200 hPa, and by 10 % to 20 % decrease of relative humidity initial data at 850-700 hPa. The result showed that the negative correlations between wind shear and intensity are found in wind shear scenarios. Wind shears of 10 ms-1 which lead to strengthening of the wind intensity in the early period of Typhoon development are found in wind shear scenarios. The effect of wind shear changes on wind intensity is statistically significant in the 20 % wind shear scenarios. During mature stage, weak wind intensity occurred when wind shear increase as given in the WRF standard-release and the 20 % decreasing scenario. Different situation is found in the 20 % increasing scenario where wind shear tend to weak and lead to strong wind intensity at this stage. Meanwhile, the reduction of moisture supply at 850-700 hPa resulting from 20 % decreasing relative humidity play a major role in the weakening of wind intensity and reduces mechanical energy of the cycle process as much as 300 J kg-1.
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