Judul: Desain Water Park di Kemang, Kabupaten Bogor
Abstrak: Meningkatnya angka jumlah penduduk di Kemang, Kabupaten Bogor menghadapi dampak yang nyata terhadap pertumbuhan penduduk menyebabkan semakin banyaknya kawasan terbangun yang tidak memiliki ruang terbuka hijau. Hal ini memperburuk kondisi keberadaan ruang terbuka hijau yang semakin hari semakin berkurang persentasenya sehingga penting bagi pemerintah maupun pihak swasta bekerja sama dalam meningkatkan ruang terbuka hijau di Kemang, Kabupaten Bogor. Menghadirkan kawasan wisata baru berupa waterpark menjadi salah satu daya tarik wisata utama di Kemang, Kabupaten Bogor yang dapat menjaga dan mempertahankan keberadaan ruang terbuka hijau. Belakangan ini, trend waterpark global yang mengutamakan nuansa alam menjadi pilihan populer bagi pengunjung yang mencari hiburan dan kesegaran. Pengembangan waterpark di Kemang mendukung pertumbuhan ekonomi dan pariwisata daerah, sejalan dengan misi pembangunan kepariwisataan Bogor. Adapun tujuan penelitian ini yaitu menganalisis kondisi eksisting berdasarkan potensi dan kendala yang terdapat pada tapak dan mendesain waterpark untuk pengembangan wisata baru di Kemang, Kabupaten Bogor. Metode yang digunakan dalam penelitian ini adalah metode deskriptif melalui survei atau pengamatan lapang, studi pustaka, dan wawancara. Pendekatan desain yang digunakan adalah program-based design. Hasil penelitian menghasilkan konsep dasar waterpark berupa “tropical paradise oasis” dengan konsep desain yang mengadaptasi bentuk bambu sebagai elemen utama dan salah satu jenis tanaman tropikal yang digunakan pada waterpark ini. Desain tapak ditujukan untuk menawarkan tempat hiburan dengan berbagai wahana air sekaligus healing, serta menawarkan pengalaman holistik yang mencakup aktivitas leisure (bersantai) dan culinary (kuliner).
Keyword: Landscape design, oasis, recreation, tropical garden, water garden

Judul: Redesign of Situ Gede Landscape as a Nature Park
Abstrak: Situ merupakan salah satu sumber daya perairan yang memberikan manfaat bagi kehidupan manusia baik dari segi ekonomi maupun lingkungan. Situ Gede, salah satu danau yang terdapat di Kota Bogor. Tujuan penelitian ini adalah mengidentifikasi dan menganalisis potensi lanskap Situ Gede dan membuat rekomendasi desain lanskap Situ Gede sebagai kawasan wisata alam. Penelitian ini mengacu pada proses perancangan Simonds (2006) dengan modifikasi penggabungan tahap akhir, yaitu tahap construction dan operation menjadi tahap proses desain. Potensi Situ Gede yaitu akses mudah dan memiliki kesan ruang terbuka hijau dan dikelilingi banyak pepohonan yang dapat menyediakan udara segar. Kendalanya adalah lahan parkir yang terbatas dan kurangnya fasilitas yang layak untuk digunakan serta sampah yang menumpuk di tepi danau. Lahan di kawasan Situ Gede potensial sebagai kawasan wisata alam. Jenis kegiatan yang diinginkan responden adalah berkemah, bersampan keliling situ dan bersepeda, kegiatan tersebut dapat dilakukan dengan menyediakan lahan khusus berkemah, memperbaiki fasilitas perahu dan menyediakan jalur untuk bersepeda. Konsep dasar yang akan diaplikasikan pada kawasan wisata danau Situ Gede adalah ekowisata dengan konsep desain, pola organik dan bentuk kujang. Hasil dari penelitian dibatasi hingga produk akhir berupa site plan, gambar potongan, gambar detail, dan ilustrasi., Situ is one of the water resources that provide benefits for human life both in terms of economy and the environment. Situ Gede, one of the lakes in the city of Bogor. The purpose of this study is to identify and analyze the potential of the Situ Gede landscape and make recommendations for the design of the Situ Gede landscape as a natural tourist area. This study refers to the design process of Simonds (2006) with the modification of combining the final stages, namely the construction and operation stages, into the design process stage. The potential there is great, namely easy access and has the impression of green open space and surrounded by many trees that can provide fresh air. The problem is the limited parking space and the lack of proper facilities to use as well as the garbage that has accumulated on the shores of the lake. The land in the area has great potential as a natural tourist area. The types of activities that respondents want are camping, canoeing around the lake, and cycling, these activities can be carried out by providing special camping areas, repairing boat facilities, and providing paths for cycling. The basic concept that will be applied to the Lake Situ Gede tourist area is ecotourism with design concepts, organic patterns, and the shape of a cleaver. The results of the research are limited to the final product in the form of site plans, cut drawings, detailed drawings, and illustrations.
Keyword: alam, lanskap, redesain, situ, wisata

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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