Judul: Transplantasi sel testikular ikan gurame Osphronemus gouramy pada ikan nila Oreochromis niloticus umur 1-4 hari
Abstrak: Giant gouramy is one of the targeted farmed species to increase its production level in the Indonesian Ministry of Maritime Affairs and Fisheries Program 2010-2014. Its production seemed slow because of the relatively long reproductive cycle. Transplantation of giant gouramy testicular cells to tilapia can be done in the hope that the surrogate broodstock (tilapia) will be able to give birth giant gouramy so the production cycle can be accelerated. In this research, transplantation of giant gouramy testicular cell (donor) in the peritoneal cavity of newly hatched tilapia (recipient) was performed. Giant gouramy testicular cells that contain spermatogonial cells obtained by dissociation of the gonad using PBS with 0.5% trypsin and then injected into tilapia with different ages. The results showed that SR (survival rate) of 1-2 days old injected larvae (A; 89.34%) were lower compared to that of 3-4 days old (B; 98.96%). Furthermore, the success rate of donor cells colonization in the recipient gonad at 2 months after injection in treatment A (6 of 6 recipients; 100%) was higher than that of treatment B (3 of 4 recipients; 75%). The results of this study indicated that transplantation using 1-2 days old larvae gave highest colonization and comparable level of SR. Thus, this technique has the potential both to manipulate gamet production of economically important fish species that relatively difficult to reproduction (commercial) and reproduce the endangered fish (as conservation).
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Judul: Xenotransplantation of giant gourami testicular germ cells into larvae of nile tilapia
Abstrak: We were attempting to develop testicular germ cell (TGC) transplantation as a tool to produce surrogate broodstock of commercially valuable fish and long generation time species. Giant gourami testis had been used as a model for donor and Nile tilapia larvae as recipient. The research was conducted to study the competency of giant gourami TGC as donor and Nile tilapia larvae as recipient for xenotransplantation of giant gouramy TGC into larvae of Nile tilapia. We developed TGC xenotransplantation system by some steps as follow : 1) The characterization of spermatogonia to identify optimal donor of giant gourami based on body weight using histological approach, 2) The determination of dissociation method for giant gourami testicular tissue by comparing two different composition of dissociation medium with 5 hours incubation time, 3) Optimizing the timing of intraperitoneal TGC transplantation into peritoneal cavity of 1, 3, 5 and 7 days post hatching (dph) recipient by investigating the colonization efficiency of donor cell labelled PKH 26 fluorescent membrane dye under fluorescent microscope and of molecular technique using growth hormone of giant gourami specific primer, 4 ) Analyzing the proliferation of spermatogonia colonized in recipient gonad using molecular approach, and 5) The evaluation of TGC isolated from testis of giant gourami which preserved at 4 oC using NaCl 0.7% for 6, 12, 24 and 48 hours and then transplanted into peritoneal cavity of recipient. The result showed that the donor with abundant spermatogonia stem cell and A type spermatogonia (cell diameter = 14.43–20.53 μm) were found in giant gourami with body weight ranged from 500–1000 g. The dissociation method produced high number of spermatogonia with high viability was one used medium PBS containing trypsin, DNase, CaCl2, HEPES, FBS and incubated for 3 hours. The highest colonization efficiency was observed at 3 dph recipient (61.1±34.71%) suggesting that 3 dph Nile tilapia larvae was the optimum recipient for transplantation. Intraperitoneally transplanted xenogenic spermatogonia efficiently colonized the ovary as well with sex ratio male out of female was 1:1, and possibly proliferated indicated by cell cluster forming and the increase of DNA concentration of donor in recipient testis during time interval 1 month to 2 or 3 month pt. The successful colonization of spermatogonia isolated from preserved testis were also observed with colonization efficiency not differed significantly as from non preserved testis. In conclusion : the testis of giant gouramy was composed of cells that had competency as donor for xenotransplantation using Nile tilapia larvae as recipient.
Keyword: Sel Testikular, Osphronemus goramy, Ikan Gurami, ikan nila, kolonisasi, proliferasi, preservasi

Judul: Analisis keamanan konstruksi fungsi hash berbasis sandi blok kunci simetrik (Kajian Teoritik)
Abstrak: Hash function is used to secure the integrity and authenticity of a massege, so that the message is save. The message which will be secured, will be attached to hash/message digest/digital signature value fro, the message. A secure hash function is the one which has pre-image resistance, 2nd pre-image resistance and collision resistance. Hash function has been classified into hash keyed function (Message Authentication Codes/MAC) and unkeyed (Modification Detection Codes/MDC)
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