Judul: The Influence of Knowledge, Awareness, Responsibility, and Environmental Attitudes on Ecological Behavior of School-Age Children
Abstrak: Permasalahan lingkungan yang masih terjadi di Indonesia adalah masalah sampah dan penggunaan energi yang berlebihan. Di sisi lain, pemerintah telah mengadakan program sekolah Adiwiyata sebagai bentuk implementasi pembelajaran tentang lingkungan hidup di ranah sekolah. Penelitian ini bertujuan untuk menganalisis pengaruh pengetahuan, kesadaran, tanggung jawab, dan sikap lingkungan terhadap perilaku ekologis anak usia sekolah. Penelitian ini menggunakan desain penelitian cross sectional study dan teknis penarikan contoh menggunakan probability sampling dengan metode cluster random sampling. Data dikumpulkan dengan membagikan kuesioner penelitian yang diisi sendiri oleh responden (self-administered) dengan pendampingan. Responden dalam penelitian ini merupakan siswa SD kelas 4, 5, dan 6 dengan jumlah 207 siswa. Analisis data deskriptif dan uji korelasi menggunakan SPSS, sedangkan untuk uji SEM menggunakan Smart PLS. Hasil uji korelasi menunjukkan bahwa terdapat hubungan antara pendidikan terakhir ayah dan ibu, serta pekerjaan ayah dengan perilaku ekologis. Analisis SEM menunjukkan bahwa tanggung jawab lingkungan berpengaruh positif signifikan secara langsung terhadap sikap lingkungan. Pengetahuan lingkungan, tanggung jawab lingkungan, dan sikap lingkungan memiliki pengaruh langsung yang positif signifikan terhadap perilaku ekologis. Perilaku ekologis anak dapat ditingkatkan dengan cara membiasakan diri mengurangi limbah sampah, membuang sampah pada tempatnya, menghemat energi listrik dan air, serta melakukan daur ulang sampah., Environmental problems that still occur in Indonesia are waste and excessive energy use. On the other hand, the government has held the Adiwiyata school program as a form of implementation of learning about the environment in the school realm. This study aims to analyze the influence of knowledge, awareness, responsibility, and environmental attitudes on the ecological behavior of school-age children. This study used a cross sectional study design and sampling technique using probability sampling with cluster random sampling method. Data were collected by distributing research questionnaires that were filled out by the respondents themselves with assistance. Respondents of this study were elementary school students in grades 4, 5, and 6 with a total of 207 students. Descriptive data analysis and correlation tests used SPSS, while the SEM test used Smart PLS. The correlation test results show that there is a relationship between the last education of the father and mother, as well as the father's occupation with ecological behavior. SEM analysis shows that environmental responsibility has a significant positive effect directly on environmental attitudes. Environmental knowledge, environmental responsibility, and environmental attitudes also have a significant positive direct effect on ecological behavior. Children's ecological behavior can be improved by getting used to reducing waste, throwing garbage in its place, saving electricity and water, and recycling waste.
Keyword: ecological behavior, environmental attitudes, environmental awareness, environmental knowledge, environmental responsibility

Judul: Green Consumption Behavior of Indonesian Young Consumers: The  Role of Knowledge, Responsibilities, and Attitudes towards the Environment
Abstrak: Konsumsi merupakan salah satu penyebab masalah lingkungan yang harus segera diatasi. Konsumen sebagai pelaku utama kegiatan konsumsi memiliki tanggung jawab utama dalam aksi penyelamatan lingkungan. Tujuan penelitian ini adalah untuk menganalisis pengaruh pengetahuan lingkungan, tanggung jawab lingkungan, dan sikap lingkungan terhadap perilaku konsumsi hijau di kalangan konsumen muda Indonesia. Penelitian ini menggunakan mix methods dengan metode pengumpulan data berupa survei online¸ wawancara mendalam, dan observasi. Sebanyak 328 responden terlibat sebagai contoh dalam penelitian menggunakan teknik voluntary sampling. Analisis data dilakukan secara deskriptif dan SEM menggunakan bantuan aplikasi SPSS 25.0 dan LISREL 8.80. Hasil penelitian ini menunjukkan pengaruh yang positif signifikan pengetahuan lingkungan terhadap tanggung jawab lingkungan, sikap lingkungan, dan perilaku konsumsi hijau. Tanggung jawab lingkungan berpengaruh positif signifikan terhadap sikap lingkungan. Sikap lingkungan berpengaruh positif signifikan perilaku konsumsi hijau. Pengetahuan, tanggung jawab lingkungan, dan perilaku konsumsi hijau konsumen muda Indonesia berada pada kategori sedang sedangkan sikap lingkungan berada pada kategori tinggi. Dengan demikian perlu peningkatan pengetahuan dan tanggung jawab konsumen terhadap lingkungan untuk mendorong peningkatan perilaku konsumsi hijau., Consumption is one of the causes of environmental problems that must be addressed immediately. Consumers as the main actors in consumption activities have the main responsibility in environmental saving actions. The purpose of this study was to analyze the effect of environmental knowledge, environmental responsibility, and environmental attitudes on green consumption behavior among young Indonesian consumers. This study uses mixed methods with data collection methods in the form of online surveys, in-depth interviews, and observations. A total of 328 respondents were involved as a sample in the study using voluntary sampling technique. Data analysis was carried out descriptively and SEM using SPSS 25.0 and LISREL 8.80. The results of this study indicate a significant positive effect of environmental knowledge on environmental responsibility, environmental attitudes, and green consumption behavior. Environmental responsibility has a significant positive effect on environmental attitudes. Environmental attitudes have a significant positive effect on green consumption behavior. Knowledge, environmental responsibility, and green consumption behavior of young Indonesian consumers are in the medium category while environmental attitudes are in the high category. Thus, it is necessary to increase consumer knowledge and responsibility towards the environment to encourage an increase in green consumption behavior.
Keyword: attitude, environmental responsibility, green consumption behavior, knowledge

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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