Judul: Komponen Kimia Fraksi Nonpolar Ekstrak Metanol Daun Trengguli (Cassia fistula)
Abstrak: Many studies have been carried out on trengguli’s leaf due to its various secondary metabolites having bioactive properties. This study covered extraction, fractionation of chemical components, and characterization of compounds contained in the isolated fractions. The extraction was done by maceration of leaves sample in methanol. Phytochemical tests on the methanol extract indicated the presence of alkaloid, flavonoid, phenolic, tannin, and steroid as the secondary metabolites. Fractionation performed by vacuum liquid chromatography method produced 7 fractions. Analysis of fraction B on thin layer chromatography (TLC) indicated a single stain in various eluents, supported by the 2-dimensional TLC and spraying with cerium sulfate results. Identification by using gas chromatograph-mass spectrometer, combined with ultraviolet-visible and Fourier transformed infrared spectrophotometer suggested that fraction B contained nonpolar compounds, including is fatty ester.
Keyword: Cassia fistula, fatty ester, trengguli leaves

Judul: Metil-p-Hidroksibenzoat dari Fraksi Nonpolar Ekstrak Metanol Daun Ketepeng (Cassia alata)
Abstrak: Ketepeng (Cassia alata) adalah tanaman perdu yang tumbuh berkelompok dan berguna bagi kesehatan. Daunnya secara tradisional digunakan untuk mengobati sembelit dan penyakit kulit di banyak negara seperti Indonesia. Penelitian secara khusus pada daun ketepeng belum banyak dilakukan di Indonesia, terutama mengenai informasi komponen kimia dalam daun tersebut. Dalam penelitian ini, metabolit sekunder daun ketepeng dari Tangerang, Provinsi Banten diisolasi menggunakan metode ekstraksi dan berbagai teknik kromatografi, seperti kromatografi cair vakum, kromatografi radial, dan kromatografi lapis tipis preparatif. Uji fitokimia ekstrak metanol menunjukkan kandungan senyawa steroid/triterpenoid, flavonoid, alkaloid, saponin, dan tanin. Sebanyak 4 subfraksi dari fraksi nonpolar ekstrak metanol dianalisis menggunakan spektrofotometer resonans magnet inti. Hasil analisis menunjukkan bahwa salah satu subfraksi dari fraksi n-heksana-etil asetat (8:2) merupakan senyawa metil p-hidroksibenzoat, sedangkan struktur molekul dari 3 subfraksi lainnya belum dapat ditentukan karena masih berupa campuran.
Keyword: Cassia alata, ketepeng, kromatografi, metil p-hidroksibenzoat

Judul: Antibody Responses against Newcastle Disease and Evaluation of Infectious Bronchitis Maternal Derived Antibody on Vaccinated Layers.
Abstrak: Information of maternal derived antibody (MDA) and onset of immunity after vaccination is important to determine appropriate vaccination programme of chickens. The aim of this study was to evaluate duration of Infectious Bronchitis (IB) MDA and evaluate onset of immunity Newcastle Disease (ND) vaccination among layers. All layers were vaccinated using live ND and Infectious Bursal Disease (IBD) at one day old age, and received Avian Rhinotracheitis (AR) vaccine at 4 day old age. Serum samples were collected at various ages: 7, 10, 14, 20, 22, 24, 29 and 35 day old. The presence of ND antibodies was measured by the hemagglutination inhibition (HI) test, whilst the presence of IB antibodies was measured by enzyme-linked immunosorbent assay (ELISA). Our study showed that ND antibodies were at positive level until 24 day old age, and reached protective level ≥ 25 only at 7 day old age, whilst IB MDA were positive up to 17 day old age. This result indicating the needs of ND booster vaccination at least at 24 day old and IB vaccination at 17 day old age.
Keyword: antibody, Infectious Bronchitis, layers, Newcastle Disease, vaccination