Judul: Karakterisasi Molekuler Tanaman Padi Nipponbare Transgenik 35S::OsERA1 yang Toleran Kekeringan
Abstrak: Drought is one of the factors that resulted in the growth and development of rice plants inhibited. One way to overcome this problem is use of transgenic rice plants that are tolerant to drought. The success of the transformation using OsERA1 gene in Nipponbare genotype rice requires further confirmation of tolerance to drought stress. The aim of this study was to obtain transgenic Nipponbare genotype rice contain gene 35S::OsERA1 drought tolerant, know the number of gene copies, and compare the contents and empty seeds with control. The evaluation of drought the tolerance was performed using Standard System Evaluation for Rice method (IRRI 1996). All tested transgenic genotypes were tolerant to drought. Molecular characterization using southern hybridization showed that transgenic rice plants had 1-3 copies of transgen. Drought stress inhibited plant growth, which lowers the amount of seed and increasing the void content. 18.5.4 and 6.4.10 genotypes are considered tolerant to drouht. 18.5.4 genotype have the number of grains per panicle contents of 36 seeds and 6.4.10 genotype have a 38.03% percentage of empty seeds
Keyword: transgenic plant, drought, southern hybridization, OsERA1 gene

Judul: Analisis Respon Toleransi Padi Nipponbare Transgenik Terhadap Salinitas Tinggi
Abstrak: The global climate change causing concerns and challenges ahead in improving the productivity of rice continuously. Increasing the temperature of the earth causes increased salinity on land around the beach due to rising sea levels. Anticipatory measures and strategically to deal with these changes is improve the nature of rice tolerant to high salinity through genetic engineering approaches. OsDREB1A and OsERF1 is a specific transcription factor genes of plants that a role in inducing other genes that responsible regulate response to stress salinity. This study aims to analyze response tolerance transgenic rice Nipponbare- OsDREB1A and Nipponbare-OsERF1 to high salinity. Analysis carried out by polymerase chain techniques reaction (PCR) using primers HPT and for selection used NaCl concentration 25 mM and 150 mM. Results treatment of 25 mM NaCl stress obtained 38 plants positive transgenic gene contains factors OsDREB1A and OsERF1 transcription of 48 plants putative transgenic with high growth characteristics of plants and root length was better than the wild type. Results selection of 150 mM NaCl stress obtained 23 plants positive transgenic of 29 plants putative transgenic with characteristics can lead a normal life when all types of wild rice dead.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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