Judul: The Activity of Microalgae Extract to RNA Helicase Inhibitor Japanese Encephalitis Virus
Abstrak: Japanese encephalitis virus is an agent of encephalitis disease. Cellular target therapy is one of the ways to prevent japanese encephalitis virus which has been recently developed. Microalgae extract assesment is potential to inhibit japanese encephalitis virus through inhibition of NS3 helicase. Microalgae were taken from Batam, Pari, and Ciater. Each microalgae has been extracted by four solvents (methanol 80%, acetone 80%, hexane 80%, and etyl acetate 80%) to produce 101 extracts. The ATPase assay was used on inhibition activity of the extracts, whereas for the toxicity method done is Brine Shrimp Lethality Test. The results showed that five microalgae extracts have a high inhibition activity which are CTR 07-09 etyl acetate 80% (97,19331%), CTR 07-10 etyl acetate 80% (95,61652%), CTR 07-01 etyl acetate 80% (95,1661%), CTR 06-08 etyl acetate 80% (94,54431%), and CTR 07-07 etyl acetate 80% (94,22955%). Then the five of microalgae extracts were tested with the toxicity test to calculate LC50 value. Microalgae extract of CTR 06-08, CTR 07-09, and CTR 07-10 have a character of non-toxic, whereas microalgae extract of CTR 07-01 and CTR 07-07 have a character of toxic.
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Judul: Isolasi dan pemurnian bahan aktif dari mikroalga BTM 11 sebagai inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus is the cause of hepatitis C disease which has high virulence. Recent therapy using combination of ribavirin and alpha interferon has short efficiency < 80%. Thus, the discovery of new drug is needed. Antiviral drugs can be discovered through molecular target therapy by finding the inhibitor of RNA helicase that play role in viral replication. Inhibitor can be derived from chemical compound produced by microalgae. The aim of this research was to isolate the active compound from microalgae BTM 11 which had inhibitory activity against RNA helicase. Inhibitory activity was measured by releasing of phospate inorganic in colorimetric ATPase assay. Crude extract was fractionated using gel filtration chromatography with methanol in chloroform solvent. The result showed that Fraction 3 has the highest inhibitory activity which (81.205%). Phytochemical test of crude extract indicated positive flavonoids and coumarin. Thin layer chromatography (TLC) analysis with eluent chloroform: methanol gave 2 spots with Rf value of 0.8 and 0.37. Moreover, high performance liquid chromatography (HPLC) analysis showed absorption peak with the highest abundance at the retention time of 12.483 minute and 16.617 minute was 266 nm and 230 nm. According to these of phytochemical, TLC, and HPLC, inhibitor compound from BTM 11 belongs to flavonoids.
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Judul: Analisis persepsi mengenai jenis pekerjaan dengan menggunakan metode penskalaan dimensi ganda
Abstrak: Persepsi seseorang terhadap pekerjaan atau anggapan kemiripan antar pasang pekerjaan, termasuk sikap dari orang tersebut. Ada lebih dari satu aspek yang membentuk sikap, karenanya sikap dikatakan memiliki dimensi ganda. Teknik pembuatan kuesioner kini semakin banyak dikembangkan karena banyak kesulitan dalam mengukur sikap atau persepsi seseorang, salah satunya adalah dengan menggunakan skala ordinal. Metode analisa peubah ganda untuk data persepsi juga semakin berkembang, di antaranya adalah metode penskalaan dimensi ganda. Penskalaan dimensi ganda merupakan suatu alat matematika yang memungkinkan kita untuk dapat mewakili kemiripan antara kedekatan atau obyek-obyek secara spasial seperti dalam sebuah peta. Obyek yang diteliti dapat diwakili oleh titik dalam ruang berdimensi dan biasanya digunakan dimensi yang serendah mungkin sehingga konfigurasi titiknya mudah dilihat dan ditafsirkan. ...
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