Judul: Regeneration of Adventitious Shoots in In Vitro Culture of Kitolod (Hippobroma longiflora (L.) G.Don)
Abstrak: Kitolod (Hippobroma longiflora (L.) G.Don) merupakan tumbuhan liar yang banyak dimanfaatkan oleh masyarakat sebagai obat tradisional. Bila pemanfaatan tanaman ini semakin berkembang, dikhawatirkan akan terjadi kekurangan ketersediaan sumber benih. Penelitian ini bertujuan mendapatkan teknik dan kondisi kultur terbaik dalam perbanyakan tanaman obat kitolod secara in vitro agar tersedia bibit yang dapat digunakan oleh masyarakat secara luas. Metode penelitian menggunakan rancangan acak lengkap (RAL) dengan dua faktor perlakuan dan 10 ulangan untuk semua percobaan kecuali pengakaran tunas adventif. Daun dan petiol kitolod digunakan sebagai bahan eksplan. Eksplan daun pada perlakuan BAP 1 mg/L + NAA 0,1 mg/L memberikan jumlah tunas adventif per eksplan paling tinggi yaitu sampai 49 tunas, namun perlakuan BAP 2 mg/L + NAA 0,1 mg/L lebih efektif dalam inisiasi dan perbanyakan tunas kitolod. Tunas-tunas pada BAP 2 mg/L + NAA 0,1 mg/L juga mampu menghasilkan tunas tertinggi (2,62±0,69 cm) pada media pembesaran dan memberikan 75% keberhasilan dalam tahap aklimatisasi. Media pengakaran terbaik adalah media MS yang ditambah NAA 0,5-1,0 mg/L., Kitolod (Hippobroma longiflora (L.) G. Don) is a wild plant that widely used by the community as a traditional medicine. If this plant is utilized more intensively, there may be a shortage of the plant due to the lack of the seed sources. This study aimed to obtain the best techniques and culture conditions for in vitro propagation of kitolod to provide a large number of planting materials for the community. The experiments were arranged using a completely randomized design (CRD) with two treatment factors and 10 replications for all experiments except shoot rooting. Leaves and petioles were used as explant sources. Leaf explants in 1 mg/L BAP + 0,1 mg/L NAA treatment produced the highest number of adventitious shoots per explant, up to 49 shoots, but 2 mg/L BAP + 0,1 mg/L NAA was more effective for shoot initiation and multiplication. The latter medium was also able to produce the tallest shoots (2,62±0,69 cm) and presented 75% of successful rate over the acclimatization period. The best rooting medium was MS medium added with 0,5-1,0 mg/L NAA.
Keyword: organogenesis, planting materials, tissue culture, traditional medicine

Judul: Induksi Proliferasi Tunas Adventif Kenaf (Hibiscus cannabinus L.) Secara In Vitro Melalui Penambahan Zat Pengatur Tumbuh 2.4-D Dan BAP
Abstrak: This research aimed to study the response of plant growth hormones 2.4-D and BAP to stimulate shoot proliferation initiation from three type of explants (meristem, meristem with cotyledon, and cotyledon) of kenaf. The research performed at Tissue Culture Research Laboratory, Agronomy and Horticulture Department, Agriculture Faculty, Bogor Agriculture University, from February to November 2010. Adventitious shoot formation on MS media supplemented with combinations of 2,4-D (0.0, 0.5, 1.0 mg/l) and BAP (0.0, 2.0, 4.0, 8.0, 10.0 mg/l). Callus began to appear in 2 weeks for all treatments on meristem and meristem with cotyledon. Results of this research showed that the medium composition without the 2.4-D + BAP had the highest number of adventitious shoots from meristem with cotyledon explants. Adventitious shoots formed after 11 weeks in culture without callus formation. The highest number of adventitious shoots resulted from the medium composition without 2.4-D +10.0 mg/l BAP. The medium composition without the 2.4-D +4.0 mg / l BAP resulted the highest number of roots from cotyledon explants. The roots of kenaf formed after 3 weeks which is marked by the emergence of roots from callus that formed on cotyledon explants.
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Judul: Metrik Einstein (Anti-) Self-Dual Berdimensi Empat dengan Metode Cartan
Abstrak: Gravitational field equation can be formulated in differential forms (differential geometry), which describes gravity as a manifestation of the curvature of space-time. Curvature of space-time is characterized by a metric that satisfies the gravitational field equation. With differential forms it can be formulated the (anti-) self-dual Einstein equation, which is a modification of the Cartan structure in (anti-) self-dual basis. Calculation of (anti-) self-dual metric with (anti-) self-dual Einstein equation is simpler than the calculation using Christoffel symbols.
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