Judul: Analisis Hubungan Penggunaan Media Sosial Facebook dengan Perilaku Konsumtif Siswa Sekolah Menengah Atas.
Abstrak: Penggunaan media sosial facebook di kalangan remaja masih cukup popular, seperti di kalangan remaja SMA Negeri 1 Ciampea. Mayoritas siswa dan gurunya memiliki media sosial facebook. Media sosial facebook digunakan tidak hanya untuk berkomunikasi namun juga menjadi market place (tempat jual beli) sehingga menimbulkan daya tarik pada kalangan remaja. Adanya berbagai macam produk yang beredar di media sosial facebook dapat mempengaruhi perilaku konsumtif pada kalangan remaja terutama pada siswa Sekolah Menengah Atas. Tujuan penelitian ini adalah mendeskripsikan penggunaan media sosial facebook di kalangan remaja serta perilaku konsumtif remaja dan menganalisis hubungan penggunaan media sosial facebook dengan perilaku konsumtif remaja. Metode penelitian ini menggunakan metode kuantitatif yang didukung dengan data kualitatif. Hasil penelitian ini menunjukkan bahwa tingkat penggunaan media sosial facebook remaja di SMA Negeri 1 Ciampea memiliki hubungan dengan perilaku konsumtif. Intensitas dalam penggunaan media sosial dapat menjadi pemicu terbentuknya perilaku konsumtif meski pada kenyataannya tingkat konsumsi remaja di SMA Negeri 1 Ciampea terbilang cukup rendah.
Keyword: Media Sosial facebook, Perilaku Konsumtif, Remaja

Judul: Hubungan Penggunaan Media Sosial dengan Perilaku Makan, Gaya Hidup Sedentari, dan Prestasi Belajar Siswa SMAN 84 Jakarta Barat
Abstrak: Penelitian ini bertujuan menganalisis hubungan penggunaan media sosial dengan perilaku makan, gaya hidup sedentari, dan prestasi belajar siswa SMAN 84 Jakarta Barat. Desain penelitian ini adalah cross sectional study melibatkan 53 subjek laki-laki dan 52 subjek perempuan kelas XI MIPA usia 15-18 tahun. Perilaku makan diukur menggunakan Food Frequency Questionnaire, asupan makanan dengan Recall 2 x 24 jam, dan gaya hidup sedentari menggunakan modifikasi Physical Activity Questionnaire for Adolescent. Hasil penelitian menunjukkan penggunaan media sosial pada hari libur lebih lama daripada hari sekolah (p=0.001). Subjek perempuan lebih tinggi daripada subjek laki-laki dalam hal sisa uang saku selain transportasi dan makan, tingkat kecukupan energi, tingkat aktivitas fisik, nilai bahasa Inggris, dan rata-rata nilai mata pelajaran (p<0.05). Uji korelasi Spearman menunjukkan terdapat hubungan signifikan antara uang saku dengan perilaku makan fast food (p=0.005; r=0.270), namun tidak terdapat hubungan signifikan antara frekuensi akses informasi mengenai makanan dari media sosial dengan perilaku makan, durasi penggunaan media sosial dengan gaya hidup sedentari, perilaku makan dan gaya hidup sedentari dengan prestasi belajar dan status gizi, serta status gizi dengan prestasi belajar (p>0.05).
Keyword: gaya hidup sedentari, media sosial, perilaku makan, prestasi belajar

Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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