Judul: The Correlation between Eating Habit, Nutrients Intake and Adequacy, Sleep Quality with Academic Achievement of Nutrition and Economics Students in IPB
Abstrak: Prestasi akademik adalah derajat keberhasilan sebagai bentuk pencapaian belajar setelah mengikuti proses belajar. Penelitian ini bertujuan menganalisis hubungan kebiasaan makan, asupan zat gizi, serta kualitas tidur dengan prestasi akademik pada mahasiswa Gizi dan Ilmu Ekonomi IPB. Desain penelitian menggunakan cross sectional dengan melibatkan 31 mahasiswa Gizi dan 30 mahasiswa Ilmu Ekonomi. Data pengetahuan gizi dikumpulkan menggunakan kuesioner yang diisi sendiri oleh subjek. Data kebiasaan makan dikumpulkan dengan food frequency questionnaires, sedangkan data asupan makanan menggunakan metode recall 2x24 jam. Data kualitas tidur dikumpulkan dengan kuesioner pittsburgh sleep quality index, data konsentrasi belajar menggunakan kuesioner digit symbol sutitution test, sedangkan data prestasi akademik didapatkan dari indeks prestasi kumulatif. Hasil penelitian menunjukkan asupan zat gizi subjek secara keseluruhan kurang dan kebiasaan makannya tidak jauh berbeda. Pengetahuan gizi mahasiswa Gizi lebih baik dibandingkan mahasiswa Ilmu Ekonomi dan terdapat perbedaan nyata (p<0,05). Mayoritas kualitas tidur subjek tergolong buruk. Konsentrasi belajar mahasiswa Gizi (72,5±7,3) lebih baik dibandingkan mahasiswa Ilmu Ekonomi (63,2±11,1) dengan perbedaan nyata (p<0,05). Prestasi akademik sebagian besar subjek tergolong baik. Terdapat korelasi yang signifikan positif antara asupan protein dan lemak dengan konsentrasi belajar serta hubungan konsentrasi belajar dengan prestasi akademik (p<0,05)., Academic achievement is degree of success as a form of learning achievement after following learning process. This research aim to analyze the correlation between eating habit, nutrients intake and sleep quality with academic achievement of nutrition and economics students in IPB. This cross sectional study involving 31 nutrition students and 30 economics students. Nutritional knowledge data was collected using a questionnaire that was filled out by the subjects themselves. Eating habit collected by food frequency questionnaires, while food intake data was collected using 2x24 hour recall. Sleep quality collected by pittsburgh sleep quality index, learning concentration collected by digit symbol substitution test, while academic achievement data was collected by grade point average. The results showed, nutrients intake from most of subject was insufficient and their eating habits were not much different. Nutritional knowledge of nutrition students was better than economics students with significant difference (p<0,05). Majority of subjects’s sleep quality classified as poor. Learning concentration of nutrition students (72,5±7,3) was better than economics students (63,2±11,1) with significant difference (p<0,05). Academic achievement of most subjects is classified as good. There is significant positive correlation between protein and fat intake with learning concentration, and correlation between concentration ability with academic achievement (p<0,05).
Keyword: academic achievement, eating habit, sleep quality

Judul: Correlation Between Stress Level, Sleep Quality, and Food Habits with Nutritional Status in IPB University Dormitory Students
Abstrak: Penelitian ini bertujuan mengetahui dan menganalisis hubungan tingkat stres, kualitas tidur, dan kebiasaan makan dengan status gizi pada mahasiswa asrama IPB. Desain penelitian ini merupakan studi cross sectional dengan jumlah subjek sebanyak 78 mahasiswa asrama IPB University. Penelitian dilakukan secara langsung pada bulan Mei 2024 melalui pengisian kuesioner dan wawancara. Instrumen pengukuran tingkat stres menggunakan kuesioner PSS-10, kualitas tidur menggunakan kuesioner Pittsburgh Sleep Quality Index (PSQI), kebiasaan makan menggunakan kuesioner Adolescent Food Habits Checklist (AFHC), status gizi diukur secara langsung pada berat badan dan tinggi badan. Sebagian besar subjek mengalami stres sedang (72%), kualitas tidur yang buruk (85%), dan kebiasaan makan yang tidak sehat (65%). Sebagian besar status gizi subjek tergolong normal (61%). Hasil analisis menunjukkan tidak terdapat hubungan signifikan antara tingkat stres, kualitas tidur, dan kebiasaan makan dengan status gizi (p>0,05)., This research aims to determine and analyze the correlation between stress level, sleep quality, and food habits with nutritional status in IPB University dormitory students. The design of this research was a cross-sectional study with 78 IPB University dormitory students as subjects. The research was conducted online in May 2024 by filling out questionnaires and interviews. Instruments for measuring stress levels using the PSS-10, sleep quality using the Pittsburgh Sleep Quality Index (PSQI) questionnaire, food habits using the Adolescent Food Habits Checklist (AFHC) questionnaire, nutritional status was measured directly on body weight and height. Most subjects experienced moderate stress (72%), poor sleep quality (85%), and unhealthy food habits (65%). Most of the subjects nutritional status was classified as normal (61%). The results of the analysis showed that there was no significant correlation between stress level, sleep quality, and food habits with nutritional status (p>0.05).
Keyword: nutritional status, sleep quality, food habits, stress level

Judul: Isolasi Konstruksi dan Rekombinasi Gen Pengkode Reverse Transcriptase asal Simian Retrovirus-2 (SRV-2)
Abstrak: The availability of reverse transcriptase enzym as a valuable component for Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique has been providing by foreign companies. Therefore, it needs more cost and time to provide it. This study is an early step to produce in house reverse transcriptase rekombinant enzym from SRV-2. The purpose of this study was isolated, constructed, and rekombined SRV-2 reverse transcriptase gene to entry clone and destination vector. Isolation was done by PCR amplification technique using three pairs of primers, which are RT I (3282-3683), RTII (3261-3900), and RTIII (3169-4133). Construction and recombination in this study used Gateway method from Invitrogen. SRV-2 reverse transcriptase gene was isolated in this study with A-549 cell inoculated SRV-2 as source of virus. This gene amplify by PCR technique and resulted specific band for RTI 401 bp, RTII 639 bp, and RTIII 964 bp. Analysis of pENTR/SD/TOPO and pDEST17 indicated SRV-2 reverse transcriptase gene code has already inserted to this plasmid. This result indicated SRV-2 reverse transcriptase gene have isolated, constructed, and recombined to entry clone and destination vector.
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