Judul: Identifikasi Molekuler Plasmodium sp. pada Macaca fascicularis dan Macaca nemestrina di Penangkaran Eksitu Jawa Barat
Abstrak: Plasmodium is a protozoan parasite that caused malaria which transmitted through the bite of an infected mosquito. A total of 27 (11.70%) positive parasites out of 316 blood samples of Macaca fascicularis and 18 positive samples from 166 blood samples of M. nemestrina were identified by microscopic examination. The objective of this study was to determine species specific based on molecular analysis using primer specifics to detect P. knowlesi, P. inui, P. fieldi, P. coatneyi and P. cynomolgi through nested Polymerase Chain Reaction, The results showed 8 out of 27 samples were positive for P. inui in M. fascicularis, whereas in M. nemestrina from 18 blood samples only 2 were positive for P. inui. The rest of microscopic positive samples were negative to the other 5 primer specifics. Based on these results, Macaca species from ex-situ captive breeding colony in West Java were positive for P. inui and this confirmed the potential zoonotic disease of Plasmodium species from M. fascicularis and M. nemestrina.
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Judul: Overview of White Blood Cell in Macaca fascicularis with Plasmodium spp Infection Status
Abstrak: Keadaan hutan Indonesia sangat cocok sebagai tempat berkembangbiak monyet ekor panjang (Macaca fascicularis) dan vektor malaria (Plasmodium spp.), serta pemanfaatan area hutan oleh manusia mungkinkan terjadinya penyebaran penyakit malaria pada manusia melalui monyet. Plasmodium spp. ditularkan dari individu ke individu lainnya melalui gigitan nyamuk Anopheles. Pada penelitian ini penggunaan monyet ekor panjang sebagai hewan model dikarenakan memiliki anatomi dan fisiologi yang sama dengan manusia. Sampel yang digunakan berupa whole blood dari Macaca fascicularis, selanjutnya dibuat ulasan darah diatas gelas objek dan diwarnai dengan Giemsa. Sampel tersebut telah dikonfrimasi status infeksi Plasmodium spp. melalui uji Polymerase Chain Reaction (PCR), selanjutnya ulasan tersebut dilakukan perhitungan diferensial leukosit. Data yang didapat dianalisis secara deskriptif dan diolah secara statistik dengan uji Mann Whitney. Hasil perhitungan diferensial leukosit menunjukkan adanya perbedaan yang nyata pada jenis neutrofil batang antara monyet ekor panjang yang positif infeksi Plasmodium spp. dengan kontrol negatif. Hasil pengamatan menunjukkan fase infeksi Plasmodium spp. yang ditemukan pada sampel darah adalah fase tropozoid, fase skizon, dan fase gametosit., The condition of Indonesia's forests is very suitable as a breeding ground for long-tailed monkeys (Macaca fascicularis) and malaria vectors (Plasmodium spp.), and the use of forest areas by humans allows the spread of malaria to humans through monkeys. Plasmodium spp. transmitted from individual to individual through the bite of the Anopheles mosquito. In this study, the use of long-tailed monkeys as animal models is due to having the same anatomy and physiology as humans. The sample used was whole blood from Macaca fascicularis, then blood smear preparations was made on an object glass and stained with Giemsa. The sample has confirmed the infection status of Plasmodium spp. through the Polymerase Chain Reaction (PCR) test, then calculated the differential leukocyte count. The data obtained were analyzed descriptively and statistically with the Mann Whitney test. The results of the differential leukocyte count showed a significant difference in the types of stem neutrophils between long-tailed monkeys that were positive for Plasmodium spp. infection with negative control. The results showed that the infection phase of Plasmodium spp. The results showed that the infection phase found in blood samples are trophozoite stage, schizont phase, and gametocyte phase.
Keyword: leukosit, monyet ekor panjang, Plasmodium spp, ulas darah

Judul: Hydroxyapatite-Chitosan Composite Coating on Stainless Steel 316 to Improve Corrosion Resistance
Abstrak: Metals that were used in bone implantation should have good corrosion resistance that were not easily corroded when implanted into the body. Metal corrosion resistance of stainless steel 316 can be enhanced through the metal coating by hydroxyapatite-chitosan composite with composition ratio of hydroxyapatite (gram) and chitosan 3% (mL) 0.2:0, 0.2:0.1, 0.2:0.5, 0.2:1.0, and 0.2:1.5. Besides improving the corrosion resistance of metal, the presence of chitosan also maintain the position of hydroxyapatite on metal surfaces. Corrosion resistance increases with increasing amount of chitosan added and the best composition of hydroxyapatite-chitosan composite was 0.2:1.0.
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