Judul: Penentuan prioritas penghijauan di desa-desa pegunungan Kabupaten Wonosobo
Abstrak: Tujuan penelitian ini adalah untuk nengetahui prioritas lokasi penghijauan yang sebaiknya dilaksanakan di desa-desa pegunungan Kabupaten Wonosabo dengan mengetahui faktor-faktor yang berpengaruh terhadap kerusakan lahan. Untuk tujuan ini telah diteliti 169 desa di Kabupaten Wonosobo yaitu desa-desa yang terletak di atas ketinggian 500 meter dpl. Untuk mengetahui faktor-faktor yang berperanan dalam menduga persamaan erosi, dipergunakan tehnik uji regresi ganda dengan cara regresi bertatar (stepwise) langkah maju (forward selection). Hasil pengujian ini nendapatkan 25 peubah yang masuk dalam persamaan dengan besarnya koefisien determinasi (R2) 70.15 persen. Dari hasil uji persiil ke-25 peubah tersebut terdapat 10 peubah yang nyata. Ke-10 faktor yang berpengaruh nyata terhadap kerusakan lahan rusak adalah (1) lereng lebih dari 40 % (2) lereng antara 15 - 40 % (3) lereng kurang dari 15 % (4) =ah hujan kurang dari 3500 mm/thn (5) curah hujan lebih dari 4000 mm/thn (6) jenis tanah dengan kepekaan erosi sedang (7) penggunaan lahan sawah (8) penggunaan lahan kebun campuran (9) penggunaan lahan perkebunan dan (10) Penggunaan lahan hutan.
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Judul: Pendugaan Tingkat Bahaya Erosi Untuk Optimalisasi Penggunaan Lahan Studi Kasus Di Desa Lamajang, Kecamatan Pangalengan, Kabupaten Bandung, Das Citarum Hulu
Abstrak: Erosion is a major cause of land degradation that lead to critical land formation. Erosion is influenced by several factor, such as : climate, soil erodibility, topograhy, land use, and conservation techniques. Among those five factors the first three factors are factors difficult to change. Therefore, erosion prevention efforts to reduce land degradation generaly focused on the regulation of land use and conservation techniques (land use optimization). Land use optimization was done by allocating any land use for a proper land use in such a way that land degradation can be prevented, i.e land use that produce erosion less than tolerable erosion. Therefore, prediction of actual and potential soil erosion as well as tolerable soil erosion level to determine erosion hazard is necessary. The method used is Universal Soil Loss Equation (USLE) which is a common method used to calculate the erosion rate. The advantages of this method are easy to apply and compatible in areas which have a rainfall and surface runoff as the main factors that causing erosion. The aim of this research is to produce a new scheme of land use based on the level of erosion hazard in order to obtain a sustainable agriculture (optimal allocation of land use) in the Lamajang Village, Sub-district Pangalengan, District of Bandung, West Java. This research includes: field observations, determination of rainfall erosivity, soil erodibility, topography, land use, land conservation techniques, and erosion hazard index. Based on data evaluation and interpretation, 7 land units are found in studied area with erosion rates ranging from low to very high. Land units with erosion hazard greater than tolerance level require land optimization. Optimization of land use activities should include the aspects of erosion, social, cultural, and economic, so make it easy to be accepted and applied for local communities The results of this study showed that erosion hazard in the studied area is very high due to high rainfall and steep slopes. Since actual soil erosion higher than tolerable level it is necessary to optimize the land use. The method of land use optimization suggested for the study area is “Good Terrace”. On areas with slope steepness higher than 60% Agroforestry system is proposed. This Agroforestry system compose of pine as high canopy, coffe or quinine as medium canopy, and chili or tomato as lower canopy (basal cover), that are planted follow contour.
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Judul: Cloning and Structure Prediction of C-Type Lectin 19 (CTL-19) from Silkworm (Bombyx mori L.)
Abstrak: Bombyx mori L. is a highly significant insect due to its silk production affects several industries. Unfortunately, B. mori farming has recently experienced mortality issues caused by pathogenic infectious diseases. Despite the extensive study of innate immunity of B. mori, information regarding the molecular functions and mode of actions of C-type lectin 19 (CTL-19) in the innate immunity of B. mori remains limited. Therefore, this study aimed to predict the structure of the B. mori CTL-19 also clone and produce the recombinant protein to better understand its role in the silkworm's innate immunity. The open reading frame (ORF) of the CTL-19 gene (945bp) was successfully amplified and cloned to pGEM®-T Easy Vector. The mature protein consists of 294 amino acids with 2 CRDs, 9 conserved cysteine, and two specific carbohydrate binding motifs. Protein threading demonstrated to be superior in order to predict the ligand binding site in comparison to homology modelling. The predicted molecular weight is 33.26 kDa, with pI value of 5.70. Furthermore, molecular docking analysis reveal that BmCTL-19 have ability to bind to various carbohydrate residues. The BmCTL-19 gene will be sub clone into an appropriate expression vector and produce purified recombinant protein to evaluate its properties.
Keyword: Innate immunity, Carbohydrate Binding Motif, Homology modelling, Protein Threading, Molecular docking