Judul: Hubungan iklim komunikasi dengan kinerja pengelola Badan Usaha Milik Desa “Tirta Mandiri”
Abstrak: Iklim komunikasi memberikan gambaran mengenai proses-proses komunikasi terjalin di dalam BUMDes sehingga menghasilkan suatu kinerja yang baik dan berujung pada peningkatan produktivitas. Tujuan penelitian ini adalah mendeskripsikan kondisi iklim komunikasi dan kondisi kinerja BUMDes, serta menganalisis hubungan iklim komunikasi terhadap kinerja pengurus BUMDes. Penelitian ini menggunakan pendekatan kuantitatif yang didukung oleh data kualitatif dengan responden sebanyak 47 orang. Unit analisis yang digunakan adalah pengelola BUMDes Tirta Mandiri di Desa Ponggok, Kecamatan Polanharjo, Kabupaten Klaten. Analisis data penelitian menggunakan uji korelasi rank Spearman. Hasil penelitian menunjukkan bahwa secara keseluruhan iklim komunikasi tidak berhubungan dengan kinerja pengelola BUMDes. Kondisi ini dikarenakan kinerja pengurus berada pada kategori menengah, sehingga tidak memiliki hubungan dengan tingginya kondisi iklim komunikasi.
Keyword: BUMDes, iklim komunikasi, kinerja

Judul: Correlation between Communication Climate and Work Effectiveness in the Development of BUMDes Business during the Pandemic (Cases: Panggung Lestari Village Owned-Enterprise, Panggungharjo Village, Sewon, Bantul, Special Region of Yogyakarta)
Abstrak: Iklim komunikasi menggambarkan suasana berlangsungnya proses komunikasi. Iklim komunikasi yang kondusif menghasilkan efektivitas kerja yang baik dan mendorong organisasi berkembang. Tujuan penelitian ini adalah menganalisis hubungan antara iklim komunikasi suportif dengan efektivitas kerja dan tingkat perkembangan usaha BUMDes di masa pandemi. Metode survei digunakan untuk memperoleh data primer dari 30 responden (empat pengurus dan 26 karyawan BUMDes). Data juga dikumpulkan melalui wawancara mendalam dengan narasumber dari Direktur BUMDes, Manajer unit usaha dan karyawan masing-masing unit usaha. Variabel yang diteliti terdiri atas iklim komunikasi suportif, tingkat efektivitas kerja dan tingkat perkembangan usaha BUMDes. Uji rank spearman digunakan untuk menganalisis data. Hasil penelitian menunjukkan terdapat hubungan nyata antara iklim komunikasi suportif (tingkat spontanitas karyawan dan tingkat keleluasaan karyawan menerima saran) dengan efektivitas kerja (tingkat prestasi kerja dan kemampuan menyesuaikan diri). Terdapat hubungan sangat nyata antara efektivitas kerja (tingkat kepuasan kerja, tingkat prestasi kerja dan kemampuan menyesuaikan diri) dengan tingkat perkembangan usaha BUMDes (tingkat kekuatan kelembagaan dan tingkat penguatan kapasitas karyawan)., Communication climate describes the atmosphere in which the communication process takes place. A conducive communication climate results in good work effectiveness and encourages the organization to develop. The purpose of this study was to analyze the relationship between a supportive communication climate with work effectiveness and the level of business development of BUMDes during the pandemic. The survey method was used to obtain primary data from 30 respondents (4 administrators and 26 BUMDes employees). Data were also collected through in-depth interviews with resource persons from the BUMDes Director, unit managers and employees. The variables studied consisted of a supportive communication climate, the level of work effectiveness and the level of BUMDes business development. Spearman rank test was used to analyze the data. The results showed that there was a significant relationship between supportive communication climate (level of spontaneity and level of freedom to accept suggestions) and work effectiveness (level of work performance and adaptability). There is a very real relationship between work effectiveness (level of job satisfaction, level of job performance and ability to adapt) with the level of business development of BUMDes (level of institutional strength and level of strengthening of employee capacity).
Keyword: business development, pandemic, suportive communication climate, work effectiveness

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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