Judul: Ekstraksi dan pemurnian protease dari Bacillus pumillus (Y1).
Abstrak: Bacillus adalah bakteri yang sangat potensial untuk dijadikan sumber enzim protease. Bacillus yang digunakan dalam penelitian ini adalah jenis B. pumillus (Y1) yang diisolasi dari limbah cair tahu (Likumahwa, 1993). Sedangkan penelitian ini bertujuan untuk melakukan ekstraksi dan pemurnian enzim protease dari B. pumillus (Y1) dengan menggunakan berbagai kolom kromatografi [DEAE (dietilaminoetil) Sephadex A-50 dan Amberlite] untuk mendapatkan enzim protease yang relatif murni. Dalam penelitian ini dilakukan fermentasi bakteri untuk menghasilkan enzim protease dalam limbah cair tahu dengan penambahan 2% skim, lalu diikuti ekstraksi enzim dengan garam ammonium sulfat 55% (w/v), besi (III) klorida, aluminium (III) klorida dan aseton dan dilarutkan kembali dalam buffer sodium sitrat, sodium ditionat dan fosfat 0.01 M pH 8.0, kemudian didialisis dalam kantung selofan. Dialisat lalu dikromatografi dalam resin penukar anion (Amberlite IRA 400 dan DEAE Sephadex A 50) dan penukar kation (Amberlite DP-1). Fraksi-fraksi yang diperoleh, dipekatkan dengan pengeringan beku dan selanjutnya dielektroforesis dalam gel SDS-poliakrilamid. ...
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Judul: Pemurnian dan karakterisasi protease dari Bacillus punilus
Abstrak: Penelitian ini bertujuan mempelajari sifat-sifat enzim protease ekstraselular dari Bacillus pumilus yang diisolasi dari limbah cair tahu di Indonesia. Galur murni yang digunakan Bacillus pumilus Y1 merupakan hasil isolasi dari Likumahua Yosi T. Maria (1993). Karakteristik enzim protease yang diamati meliputi pengaruh suhu, pH, jenis dan konsentrasi ion logam terhadap aktivitas enzim, param- eter kinetika reaksi ( Km dan Vmax ), dugaan berat molekul, dan tingkat kemurnian yang dapat dicapai setelah pemurnian melalui pengendapan garam, dialisis dan kromatografi. Produksi enzim dilakukan dengan proses fermentasi sistem curah ( batch). Limbah cair tahu sebanyak 800 ml (pH 7), ditambah 5% susu skim digunakan sebagai medium. untuk pertumbuhan Bacillus pumilus. selam 12 jam Fermentasi dilakukan dalam bejana fermentor pada suhu 37° C, dengan agitasi 100 rpm, dengan aerasi 1 VVM dan pH 7. Pemanenan enzim protease dilakukan dengan sentrifugasi pada 2000 rpm selama 20 menit pada suhu 0° C. Supernatan yang dihasilkan disebut ekstrak kasar…dst
Keyword: Protease, Bacillus punilus, Microbial enzym, Permentation

Judul: Thermal Characteristics and Dynamics of Tropical Cyclone over Western North Pacific Ocean: A Case Study of Typhoon Songda (Chedeng) 2011
Abstrak: The frequent occasion of Tropical Cyclone (TC) over Western North Pacific (WNP) Ocean lies from 10˚N to 26˚N and 121˚E to 170˚E. Sources of TC development come from warm ocean temperature where latent heat is released during condensation processes and subsequently concentrated in the boundary layer. Relative humidity at 850-700 hPa is responsible to produce the most intense storm. Meanwhile, weak vertical wind shear between 850-200 hPa is one of the dynamical factors that lead to the development of TC. Pressure level of 850-200 hPa is chosen to minimize the thermodynamic effect in the lower troposphere. Typhoon Songda (Chedeng in the Philippines) or TSC lasted from May 18th to 30th, 2011 and strengthened into powerful Super Typhoon stage between May 24th and 27th. Long curving track and the exhibition of all sequences of TC life cycle, TSC is then considered as an ideal case of TC. The objective of the study with case of TSC is to investigate the role of thermal characteristics and dynamics on TC intensity. The research is carried out by using Advanced Research of Weather Research and Forecasting model (WRF ARW model) version 3.3. Two domains are employed in the model. The first domain situate from 02˚N to 40˚N and 118˚E to 149˚E and cover spatial resolution () of 33.1 km. The second domain situate from 09˚N to 27˚N and 119˚E to 132˚E with of 11 km. Timestep of the model () is 120 seconds. The model is supported by the following physical parameterization, i.e., Yonsei University Scheme (YSU) in the boundary layer, Kain-Fritsch scheme (KF) in the cumulus, and WRF Single-Moment 3-class scheme in the microphysics. Initial meteorological condition prior to the formation of the cyclone is generated using WRF ARW standard-release model. Sensitivity of the model output is obtained from a selected single-parameter change scenarios, namely, 10 % to 20 % decrease and increase of wind shear initial data at 850-200 hPa, and by 10 % to 20 % decrease of relative humidity initial data at 850-700 hPa. The result showed that the negative correlations between wind shear and intensity are found in wind shear scenarios. Wind shears of 10 ms-1 which lead to strengthening of the wind intensity in the early period of Typhoon development are found in wind shear scenarios. The effect of wind shear changes on wind intensity is statistically significant in the 20 % wind shear scenarios. During mature stage, weak wind intensity occurred when wind shear increase as given in the WRF standard-release and the 20 % decreasing scenario. Different situation is found in the 20 % increasing scenario where wind shear tend to weak and lead to strong wind intensity at this stage. Meanwhile, the reduction of moisture supply at 850-700 hPa resulting from 20 % decreasing relative humidity play a major role in the weakening of wind intensity and reduces mechanical energy of the cycle process as much as 300 J kg-1.
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