Judul: Keragaman Gen HSPG2 dan Asosiasinya terhadap Asam Lemak pada Ayam F2 Persilangan Kampung Broiler
Abstrak: Gen HSPG2 merupakan salah satu gen yang berperan penting dalam pemeliharaan dan fungsi jaringan adiposa dan serat otot. Penelitian ini bertujuan menganalisis keragaman gen HSPG2 serta asosiasinya dengan asam lemak pada ayam F2 persilangan kampung broiler. Total sampel yang digunakan sebanyak 62 sampel DNA ayam F2 persilangan kampung broiler. Identifikasi keragaman gen HSPG2|BsaHI dianalisis menggunakan PCR-RFLP. Produk amplifikasi memiliki panjang sebesar 356 bp. Gen HSPG2 bersifat polimorfik dengan menghasilkan 3 genotipe yaitu CC, CT, dan TT. Hasil chi-kuadrat menunjukkan gen HSPG2 berada dalam kesetimbangan Hardy-Weinberg. SNP gen HSPG2 posisi g.6574957 C>T pada genotipe CC berasosiasi (P<0,05) berasosiasi dengan asam lemak jenuh yaitu asam laurat (C12:0); asam palmitat (C16:0), asam lemak tidak jenuh tunggal yaitu asam oleat (C18:1n9c), asam lemak tidak jenuh ganda yaitu asam eikosedoneat (C20:2) dan total asam lemak. SNP gen HSPG2 disimpulkan berpotensi digunakan sebagai penanda genetik dalam program seleksi yang menghasilkan daging ayam dengan kandungan asam lemak tak jenuh tinggi.
Keyword: asam lemak, ayam, Gen HSPG2, PCR-RFLP, Nadira Andarini Siregar

Judul: Identifikasi Keragaman Gen Stearoyl-CoA Desaturase (SCD|HinfI) pada Ayam Silangan Kampung dan Broiler dengan Teknik PCR-RFLP
Abstrak: Gen Stearoyl-CoA Desaturase (SCD) merupakan salah satu gen pengode enzim yang terlibat dalam biosintesis asam lemak. Penelitian ini dilakukan untuk mengidentifikasi keragaman gen SCD pada generasi kedua persilangan kampung-broiler (KBKB) dengan menggunakan metode Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Sampel DNA yang digunakan untuk identifikasi keragaman sebanyak 100 sampel DNA ayam yang terdiri atas 62 sampel ayam KBKB, 20 sampel DNA ayam broiler dan 18 sampel DNA ayam kampung. Perhitungan genotip, frekuensi alel, nilai heterosigositas, dan keseimbangan Hardy-Weinberg pada penelitian ini dilakukan pada ayam persilangan kampung dan broiler. Gen diamplifikasi pada posisi c.17492542G>C dengan panjang sekuen 407 bp dan dipotong menggunakan enzim restriksi HinfI menghasilkan ; 373 dan 34 bp pada genotip GG; 407, 373 dan 34 bp pada genotip GC, serta 407 pada genotip CC. Frekuensi alel tertinggi ditemukan pada alel G. Genotip GG dan GC lebih banyak ditemukan dibandingkan genotip CC pada populasi ayam KBKB. Hasil identifikasi gen SCD|HinfI menunjukkan bahwa populasi ayam KBKB, ayam broiler dan ayam kampung yang digunakan bersifat polimorfik (beragam) dan berada pada keseimbangan Hardy-Weinberg. SCD merupakan gen kandidat yang potensial mengontrol biosintesis asam lemak ayam.
Keyword: heterosigositas, keragaman, PCR-RFLP, SCD

Judul: Thermal Characteristics and Dynamics of Tropical Cyclone over Western North Pacific Ocean: A Case Study of Typhoon Songda (Chedeng) 2011
Abstrak: The frequent occasion of Tropical Cyclone (TC) over Western North Pacific (WNP) Ocean lies from 10˚N to 26˚N and 121˚E to 170˚E. Sources of TC development come from warm ocean temperature where latent heat is released during condensation processes and subsequently concentrated in the boundary layer. Relative humidity at 850-700 hPa is responsible to produce the most intense storm. Meanwhile, weak vertical wind shear between 850-200 hPa is one of the dynamical factors that lead to the development of TC. Pressure level of 850-200 hPa is chosen to minimize the thermodynamic effect in the lower troposphere. Typhoon Songda (Chedeng in the Philippines) or TSC lasted from May 18th to 30th, 2011 and strengthened into powerful Super Typhoon stage between May 24th and 27th. Long curving track and the exhibition of all sequences of TC life cycle, TSC is then considered as an ideal case of TC. The objective of the study with case of TSC is to investigate the role of thermal characteristics and dynamics on TC intensity. The research is carried out by using Advanced Research of Weather Research and Forecasting model (WRF ARW model) version 3.3. Two domains are employed in the model. The first domain situate from 02˚N to 40˚N and 118˚E to 149˚E and cover spatial resolution () of 33.1 km. The second domain situate from 09˚N to 27˚N and 119˚E to 132˚E with of 11 km. Timestep of the model () is 120 seconds. The model is supported by the following physical parameterization, i.e., Yonsei University Scheme (YSU) in the boundary layer, Kain-Fritsch scheme (KF) in the cumulus, and WRF Single-Moment 3-class scheme in the microphysics. Initial meteorological condition prior to the formation of the cyclone is generated using WRF ARW standard-release model. Sensitivity of the model output is obtained from a selected single-parameter change scenarios, namely, 10 % to 20 % decrease and increase of wind shear initial data at 850-200 hPa, and by 10 % to 20 % decrease of relative humidity initial data at 850-700 hPa. The result showed that the negative correlations between wind shear and intensity are found in wind shear scenarios. Wind shears of 10 ms-1 which lead to strengthening of the wind intensity in the early period of Typhoon development are found in wind shear scenarios. The effect of wind shear changes on wind intensity is statistically significant in the 20 % wind shear scenarios. During mature stage, weak wind intensity occurred when wind shear increase as given in the WRF standard-release and the 20 % decreasing scenario. Different situation is found in the 20 % increasing scenario where wind shear tend to weak and lead to strong wind intensity at this stage. Meanwhile, the reduction of moisture supply at 850-700 hPa resulting from 20 % decreasing relative humidity play a major role in the weakening of wind intensity and reduces mechanical energy of the cycle process as much as 300 J kg-1.
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