Judul: Vulnerability Analysis of E-Voting System Pilkada Kota Bogor using Attack Trees.
Abstrak: Conventional elections process had some weakness such as double voting and the length of time recapitulation. Furthermore that cost’s and relatively large resources. E-voting is one alternative to replace election. The main of problem in e-voting is safety factor, therefore be required a good method for identification and vulnerability analysis. Attack trees give way to simplify the task of vulnerability analysis. Purpose of this research is doing vulnerability analysis in e-voting system pilkada in bogor city. This research include the description of e-voting system pilkada in bogor city, defining secure voting requirement and vulnerability analysis to the components of the e-voting system using the method of attack trees. This analysis oriented e-voting special attack that aims to influence outcome of the election is not to sabotage election. Qualitative analysis results showed that there is a vulnerability in the e-voting system Bogor city pilkada election.
Keyword: attack trees, elections, e-voting, vulnerability

Judul: Desain E-Voting Pilkada Kota Bogor Menggunakan Protokol Two Central Facilities yang Dimodifikasi
Abstrak: Penerapan sebuah protokol harus sesuai dengan kebijakan-kebijakan yang berlaku protokol tersebut akan diterapkan agar dapat diterima dan diterapkan sesuai dengan keadaan. Desain sistem e-voting menggunakan protokol Two Central Facilities yang dimodifikasi untuk pilkada Kota Bogor masih berupa rancangan dasar yang harus dikembangakan lebih lanjut agar dapat diterapkan secara langsung untuk pilkada Kota Bogor, sebab masih banyak hal-hal yang harus dipertimbangkan untuk menyempurnakan sistem e-voting baik itu faktor internal, eksternal, maupun kebijakan yang mendasari sistem e-voting.
Keyword: Bogor e-voting, E-voting, E-voting protocol, Voting machine

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable