Judul: Pengembangan Kultur Primer Hepatosit Tupaia javanica Sebagai Media Isolasi dan Deteksi Virus Hepatitis B
Abstrak: Human hepatitis B virus is a major health problem worldwide which have a very narrow host range. The susceptibility of Tupaia belangeri hepatocytes primary culture has been used as an alternative in vitro model for studying hepatitis B virus infection. In this research, we proposed the usage of T. javanica hepatocytes primary culture as an in vitro model to isolate and detect hepatitis B virus from human and Hylobates moloch. Both in vitro infection of the hepatitis B virus in T. javanica hepatocytes primary culture show viral DNA replication in Real Time PCR. The result suggest that T. javanica may become a useful model for studying hepatitis B virus infecton
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Judul: Isolasi, Identifikasi dan Karakterisasi Molekular Virus Hepatitis B pada Owa Jawa (Hylobates moloch) di Indonesia
Abstrak: Hepatitis B virus (HBV) was reported not only able to infect human being, but also non-human primates, especially the great apes group such as orangutans, gorillas, chimpanzees, as well as the lesser apes from the family of Hylobatidae. Among other species within the genus, Hylobates moloch (owa jawa or silvery/ javan gibbons), which is considered as one of Indonesia’s endemic endangered species, has been reported to harbor HBV of their own strain. Analyses of HBV isolated from infected gibbons were done in this study to reveal their relatedness to the published data. Plasma samples from nine javan gibbons were obtained as part of diagnostic purpose. Those animals were previously tested positive for the presence of hepatisis B-surface antigen (HBsAg) by enzyme-linked immunosorbent assay (ELISA). DNA samples were extracted from these nine plasma samples to be used as templates for amplification of the Pre-S1 region by polymerase chain reactions (PCR) technique using semi degenerate primers that could amplify several strains of HBVs. The PCR products of 457 - 459 base pairs were then subject to restriction fragment length polymorphism (RFLP) analyses using Bst2UI enzyme to evaluate the pattern of cleaved fragments. The PCR products were sequenced for further homology analyses and to create phylogenetic tree of the sequenced data obtained from the study in comparison with published data. Two plasma samples were used as templates for whole region amplification using sets of 4 primer. The PCR products of 3192 base pairs were sequenced for further homology analyses and to create phylogenetic tree. Phylogenetic tree analyses for Pre-S1 nucleotide region and whole region showed that the javan gibbon isolates formed their own clusters, separate from the other nonhuman primate isolates.
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Judul: Pelabelan Super Edge Magic pada Graf Cycle dan Graf Wheel
Abstrak: This manuscript proves that cycle graph and wheel graph have a super edge magic labeling. Super edge magic labeling on a graph is labeling that has an edge magic labeling with a set of vertices were mapped in to {1,2,… ,𝑝} and a set of edges were mapped in to {𝑝+1,𝑝+2,… ,𝑝+𝑞}, in which 𝑝 is order and 𝑞 is size on the graph. There are one lemma and two theorems to be discussed. The lemma is used to prove the two theorems. The first theorem proves that cycle graph 𝐶𝑛 is super edge magic if and only if 𝑛 is odd. The second theorem proves that wheel graph 𝑊𝑛 of order 𝑛 is not super edge magic. Moreover 𝑊𝑛 is not edge magic if 𝑛≡0 mod
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