Judul: The Study of Vanilla’s Water Uptake (Vanilla Planifolia A.) while Experiencing Terrestrial Root Dysfunction
Abstrak: Tanaman vanili sering kali terserang penyakit yang menyebabkan jaringan perakaran utama menjadi terputus sehingga fungsi mensuplai air dan hara ke daun terhenti. Penelitian dilakukan untuk mengevaluasi peran serapan air pada akar udara serta implikasinya terhadap pertumbuhan dan status air dalam jaringan saat tanaman vanili mengalami gagal fungsi akar utama. Penelitian dilaksanakan di Rumah Kaca Balittro pada periode Juli 2021-April 2022. Penelitian menguji faktor tunggal (pemberian akses serapan air pada akar udara saat tanaman vanili mengalami gagal fungsi akar utama) menggunakan desain Rancangan Kelompok Lengkap Teracak (RKLT) dengan ulangan sebanyak lima kali. Berdasarkan hasil penelitian, pemberian akses serapan air pada akar udara berpengaruh sangat nyata terhadap pertumbuhan tajuk. Tanaman dengan akses serapan air akar udara terbukti dapat menjaga pertumbuhan sulur dan daun dengan baik hingga pekan ke-8 setelah mengalami gagal fungsi akar utama, dibandingkan tanaman yang tanpa akses serapan air pada akar udara yang mengalami stagnasi tumbuh pada pekan ke-5. Di sisi lain, variasi respons perubahan status air jaringan tidak terdeteksi secara nyata sepanjang pengujian., Vanilla plants are often attacked by diseases causing the terrestrial root becomes disconnected so that the function of supplying water and nutrients to the leaves is stopped. This study aimed to evaluate the role of water uptake in aerial roots and its implications for growth and water status in tissues when vanilla plants experienced terrestrial root dysfunction. The research was carried out in the Balittro greenhouse in the period July 2021-April 2022. The study used a single factor (providing access to water uptake in aerial roots when the vanilla plant experienced terrestrial root dysfunction) in the Randomized Complete Block Design (RCBD) and was replicated 5 times. The results showed that providing access to water uptake in aerial roots had a very significant effect on crown growth. Plants with access to water uptake in aerial roots were proven to be able to maintain the growth of stem and leave well up to 8 weeks after experiencing terrestrial root dysfunction, compared to plants without access to water uptake in aerial roots which experienced stagnation in growth at 5 weeks. On the other hand, variations in the response to changes in tissue water status were not detected significantly throughout the research.
Keyword: Vanili, Gagal Fungsi Akar Utama, Pertumbuhan, Status Air Jaringan

Judul: Induksi Kalus dan Regenerasi Tunas Vanili (Vanilla planifolia Andrews) Varietas Unggul secara In Vitro
Abstrak: Vanili (Vanilla planifolia Andrews) merupakan tanaman yang secara komersil dibudidayakan dan dieksploitasi dalam skala besar. Perbanyakan vanili secara generatif sukar dilakukan, sedangkan secara vegetatif melalui stek batang masih sangat terbatas dan rentan terhadap penyebaran cendawan patogen penyebab busuk batang vanili (BBV). Perbanyakan vanili secara in vitro menjadi upaya alternatif dalam memenuhi ketersediaan benih vanili bebas penyakit. Penelitian ini bertujuan mendapatkan kombinasi auksin dan sitokinin yang terbaik untuk memperoleh kalus dan regenerasinya untuk perbanyakan tanaman vanili varietas unggul dengan ketahanan lebih tinggi terhadap BBV. Metode penelitian ini menggunakan rancangan acak lengkap (RAL) dengan dua faktor perlakuan. Kombinasi 2,4-D yang lebih tinggi dari kinetin digunakan untuk menginduksi kalus, sedangkan kombinasi 2,4-D dengan kinetin yang ditinggikan diterapkan pada metode pembesaran kalus; kedua tahap ini dilakukan tanpa cahaya. Perlakuan 2,4-D (0,5 - 1,5 mg/L) + kinetin (0,1 mg/L) merupakan kombinasi terbaik dalam menginduksi kalus pada eksplan buku pertama dan pucuk. Kombinasi 2,4-D (0,1 mg/L) + kinetin (0,5 - 1 mg/L) efektif dalam proliferasi dan pembentukan kalus organogenik. Regenerasi kalus organogenik menjadi tunas di bawah cahaya pada media dengan BAP 0,5 mg/L menghasilkan regenerasi tunas dan bakal tunas terbanyak., Vanilla (Vanilla planifolia Andrews) is a commercial plant cultivated and exploited on a large scale. Sexual propagation of vanilla is difficult to carry out, while vegetative way through stem cuttings is still very limited and vulnerable against the spread of pathogenic fungi causing vanilla stem rot (SRD). Micropropagation is an alternative effort to provide disease-free vanilla seedlings. This study aimed to obtain the best combination of auxin and cytokinin for callus and its regeneration to provide plantlets of vanilla superior variety with higher resistance to SRD. This research used a completely randomized design (CRD) with two factors. A combination of 2,4-D which is higher than kinetin was used to induce callusing, while the combination of 2,4-D with higher concentration of kinetin was applied for callus proliferation; both steps were done in the dark. The treatments of 2,4-D (0,5 - 1,5 mg/L) + kinetin (0,1 mg/L) were the favorable in inducing callus on first node and apical shoot explants. The combinations of 2,4-D (0,1 mg/L) + kinetin (0,5 - 1 mg/L) were effective for callus proliferation and organogenic callus formation. Organogenic callus regeneration shoots under the light on media with BAP 0,5 mg/L produced the most abundant regenerated shoots and buds.
Keyword: BAP, kinetin, organogenic callus, photomorphogenesis, 2,4-D

Judul: Penentuan Nilai Eigen dan Vektor Eigen dari Matriks Tridiagonal 2-Toeplitz dengan Pendekatan Polinomial Chebyshev
Abstrak: The eigenvalues and eigenvectors of a matrix can be determined by finding its characteristic polynomials. The characteristic polynomials of a tridiagonal 2-Toeplitz matrix is shown to be closely connected to polynomials which satisfy the Chebyshev recurrence relationship. If the order of the matrix is odd, then the eigenvalues are found explicitly in terms of the Chebyshev zeros and the eigenvectors are found in terms of the polynomials satisfying the recurrence relationship. For even ordered matrices, the situation is more complicated. The problem in these cases is that although the Chebyshev recurrence formula is still applied, its initial values are not generating Chebyshev polynomials
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