Judul: Pemurnian Parsial dan Karakterisasi Beta-Galaktosidase Lactobacillus plantarum B 123
Abstrak: Lactose intolerance was a problem faced by a large proportion of the world's population. These problem could be alleviated by hydrolyzing lactose to glucose and galactose by β-galactosidase. β-Galactosidase occurred widely in nature and it was produced by a number of microorganisms. However, their characteristics were very different and it varied with the source of microorganism. The aim of this study was purification and characterization of β-galactosidase L. plantarum B 123. β-Galactosidase was extracted and partially purified by ammonium sulfate precipitation and dialysis. These steps resulted a purification level of 7.61 fold, a yield of 10.67%, and a specific activity of 61.53 U/mg protein. Optimum temperature and pH for dialyzed β-galactosidase were optimized at 50 °C and 7.0, respectively. Metal ions of Mg2+, Mn2+, Co2+, and Zn2+ in 10 mM stimulated the activity of crude β-galactosidase, while metal ions of Cu2+, Hg2+, and Ca2+ in 10 mM inhibited the activity of crude β-galactosidase. KM and Vmax for this dialyzed β-galactosidase were 1.13 mM and 7.25 U/mL, respectively.
Keyword: Bogor Agricultural University (IPB), characterization, dialysis, Lactobacillus plantarum B 123, β-galactosidase

Judul: Purifikasi Parsial Dan Karakterisasi β-Galaktosidase Dari Isolat Bakteri Asam Laktat (BAL) Strain B134
Abstrak: Lactose intolerance is often occurs in baby, adult, and eldery. It could happen because of the absence of β-galactosidase in gastrointestinal tract. One of solution is β-galactosidase intake from other sources. LAB strain B134 Isolate is a source of β-galactosidase. The aim of the present study was to partial purification and characterization of β-galactosidase from LAB strain B134 isolate. Maximum production of β-galactosidase was obtained when 2% inoculums in MRS that contain 2% lactose, pH: 5, and incubation for 24 hours in 37ºC. Partial purification of β-galactosidase used dialysis. The purification factor, yield, and specific activity of β-galactosidase were found to be 3.313 times, 3.026%, and 45.828 U/mg respective. β-galaktosidase showed higest activity was at pH 6 and temperature 45ºC. Activity of β-galactosidase was stable at pH 4.5 and 30ºC. Cation Co2+, Zn2+, Mn2+, Mg2+ in 0.1 M increased β-galactosidase activity. Meanwhile, Ca2+, Cu2+, and Hg2+ in 0,1 M inhibited β-galactosidase activity. Km and Vmaks of β-galactosidase were 3.934 mM and 3.278 μmol/min.
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Judul: Warna bulu kucing ras Persia : variasi pola warna dan pewarisannya
Abstrak: Kucing Persia merupakan kucing ras yang paling umum dijumpai di Indonesia. Kucing Persia memiliki bulu yang panjang dengan variasi warna bulu yang sangat beragam. Perkawinan yang lebih terarah sangat perlu dilakukan untuk meningkatkan kualitas genetik warna dan pola warna bulu kucing. Penelitian ini bertujuan untuk mempelajari variasi warna dan pola warna bulu kucing Persia yang terdapat di Indonesia, yang dilakukan di kantor pusat Cat Fancy Indonesia mulai bulan Oktober 2000 sampai Januari 2001. Data yang digunakan merupakan data pedigree kucing Persia yang tercatat di Cat Fancy Indonesia dari tahun 1997 sampai Oktober 2001 yang terdiri dari 187 jantan dan 258 betina, selain itu juga dilakukan pengamatan langsung kepada tiga penyayang dan breeder kucing yang ada di Bandung. Secara umum tercatat 104 penyayang dan breeder kucing ras di Indonesia, 74,04% terdapat di Jawa Barat dan sisanya (25%) terdapat di daerah Jakarta. Jumlah kucing Persia yang lahir di Indonesia adalah 387 kucing (87,16%), yang terdiri dari 163 (42,12%) jantan dan 224 (57,88%) betina. Jumlah kucing Persia yang diimpor ke Indonesia adalah sebanyak 57 (12,845%) yang terdiri dari 23 (40,35%) jantan dan 34 (59,65%) betina. Jumlah kucing Persia yang terdapat di Jawa Barat (84,14%) lebih banyak dibanding jumlah kucing Persia yang ada di Jakarta (14,86%). Secara umum warna bulu cream (9,01%) dan pola warna solid (34,01%) memiliki persentase tertinggi. Warna cream merupakan warna dilusi dari warna merah (red) yang dapat dihasilkan dari berbagai macam kombinasi warna bulu yang dimiliki tetua sebelumnya, sebagai contoh dari perkawinan tetua yang memiliki warna bulu cream dengan red smoke. Perkawinan antara tetua yang memiliki warna dan pola warna bulu yang tidak murni dapat menghasilkan turunan yang memiliki warna dan pola warna bulu yang bervariasi.
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