Judul: Kebutuhan pelatihan dalam rangka pengembangan kemampuan peternak domba (kasus di desa Gunung Sari, kecamatan Pamijahan, kabupaten Bogor)
Abstrak: Research is aimed identify the following items in Gunung Sari Village Kecamatan Pamijahan kabupaten Bogor; 1) characteristic of sheep farmer. 2) characteristics of livesyock effort, 3) training needs in sheep livestock, 4) relationship between shepp family and sheep livestock in developing traning needs. Questionnaire is the method of data collection that was done in June 2006.
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Judul: Kasus Peternak Domba di Kelurahan Cimahpar, Kecamatan Bogor Utara, Kota Bogor, Jawa Barat
Abstrak: Cimahpar’s Village represent one of a village in Bogor City which some of its society are sheep farmers. Motivation of sheep farmers is important aspect in livestock development. The research aims are needed the sheep farmer characteristics, motivational level of sheep farmers, and influencing of motivation level factors of sheep farmers in Cimahpar’s Village, Subdistrict of North Bogor, Bogor City, West Java. The research conducted on June-July 2005 at Cimahpar’s Village, Subdistrict of North Bogor, Bogor City, West Java. The total of sheep farmers as population are 390 farmers. Sample take by simple random sampling, totaly 63 farmers. The results indicated that the motivation of sheep farmers in Cimahpar’s Village categorized medium. It’s mean that sheep farmers in Cimahpar’s Village have motivation to manage sheep livestock, including to increase sheep productivity. Those motivation influencing by age, number of family members, education and knowledge of market information. This research recommended as follow. Sheep farmer need facilitate to build group farmer sheep that orientate to increase sheep farmer economic condition. The institute expected able to assist the sheep farmer in marketing and on farm aspect, including livestock produce accomplishment. All the activity will be more succeed if followed with the activity of empowerment of sheep farmer.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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