Judul: The Interrelation between Stress Level, Quality of Diet, Nutritional Status, and Academic Achievement of Students, The Interrelation between Stress Level, Quality of Diet, Nutritional Status, and Academic Achievement of Students
Abstrak: Konsumsi pangan yang beragam dan bergizi merupakan hal yang penting bagi status gizi normal serta fungsi otak agar dapat memiliki prestasi belajar yang baik. Penelitian ini bertujuan menganalisis keterkaitan tingkat stres, kualitas diet, status gizi, dan prestasi belajar SMA. Penelitian cross-sectional ini melibatkan 82 subjek yang dipilih secara purposive. Pengumpulan data tingkat stres dilakukan melalui pengisian kuesioner oleh subjek, kualitas diet diestimasi menggunakan Indonesian Healthy Eating Index (I-HEI), berat badan dan tinggi badan diukur secara langsung untuk mengestimasi status gizi. Sebagian besar subjek memiliki tingkat stres tinggi (62,2%), kualitas diet dengan kategori membutuhkan perbaikan atau need improvement (58,6%), dan status gizi normal (62,2%). Tidak terdapat hubungan signifikan (p>0,05) antara tingkat stres dengan kualitas diet (p=0,320, r=-0,111) dan prestasi belajar (p=0,236, r=0,039); antara kualitas diet dengan status gizi (p=0,618, r=0,056); serta antara status gizi dengan prestasi belajar (p=0,180, r=0,150). Namun demikian, seluruh subjek yang gemuk (overweight dan obes) memiliki kualitas diet yang tidak baik (buruk dan membutuhkan perbaikan). Subjek dengan prestasi belajar baik cenderung memiliki status gizi baik (60,9%) dan tingkat stres tinggi (56,5%). Hal ini mengindikasikan bahwa subjek dengan status gizi baik lebih berpeluang memiliki prestasi belajar yang baik pula., The consumption of varies and nutritious foods is important for normal nutritional status and brain function to improve academic achievement. This study aimed to analyze the correlation between stress levels, the quality of diet, nutritional status, and high school academic achievement. This cross-sectional study involved 82 subjects selected by purposively. Stress level data was collected through filling out questionnaires by subjects, diet quality was estimated by Indonesian Healthy Eating Index (I-HEI), body weight and height were measured directly to estimate nutritional status. Most subjects had high level of stress (62,2%), diet quality with the category need improvement (58,6%), and normal nutritional status (62,2%). There was no significant correlation (p>0,05) between stress levels with diet quality (p=0,320, r=-0,111) and academic achievement (p=0,236, r=0,039); between diet quality with nutritional status (p=0,618, r=0,056); between nutritional status with academic achievement (p=0,180, r=0,150). However, all subjects who were overweight and obese had either poor or need improvement their diet quality. Subjects with good academic achievements tended to have a good nutritional status (60,9%) and high stress levels (56,5%). This indicated that subjects with good nutritional status were more likely to have a good academic achievement as well.
Keyword: Academic Achievement, Diet Quality, Nutritional Status, Stress Level

Judul: Factors related to Stress Levels and Quality of Food Consumption of Overweight and Normal Hight school Adolecents during the Pandemic Period
Abstrak: Masa pandemi Covid-19 menimbulkan pembatasan aktivitas di luar rumah termasuk belajar secara daring yang memengaruhi stres akademik, konsumsi pangan dan gaya hidup (kebiasaan makan, kegiatan daring, aktivitas fisik). Penelitian ini bertujuan menganalisis faktor-faktor yang berhubungan dengan tingkat stres dan kualitas konsumsi pangan remaja SMA dengan status gizi overweight dan normal pada masa pandemi. Desain penelitian ini adalah comparative cross sectional study dengan subjek sebanyak 41 remaja dengan status gizi normal dan 41 remaja overweight. Subjek dipilih secara purposive dengan pengumpulan data primer melalui kuesioner dan wawancara. Berdasarkan hasil uji beda Mann-Whitney dan Independent Sample T-Test terdapat perbedaan yang signifikan (p<0,05) pada variabel waktu kegiatan daring (p=0,041), tingkat kecukupan energi (p=0,040), frekuensi konsumsi makanan pokok (p=0,004), protein nabati (p=0,021), dan minuman (p=0,003) antara subjek normal dan overweight. Hasil uji korelasi Spearman menunjukkan terdapat hubungan yang signifikan positif (p<0,05) antara tingkat kecukupan energi, protein, lemak, karbohidrat, dan vitamin C dengan kualitas konsumsi pangan. Variabel jenis kelamin berhubungan signifikan (p<0,05) dengan tingkat stres. Berdasarkan uji korelasi Spearman, tidak terdapat hubungan yang signifikan (p>0,05) antara tingkat stres dan kualitas konsumsi pangan dengan status gizi., The Covid-19 pandemic has caused restrictions on activities outside the home including online learning which affects academic stress, food consumption and lifestyle (eating habits, online activities, physical activity). This study aims to analyze factors related to stress levels and quality of food consumption Hight school Adolecent with overweight and normal nutritional status during the pandemic Period. The design of this study was comparative cross sectional study of 41 adolescents with normal nutritional status and 41 overweight adolescents selected by purposive sampling. Data was collected through questionnaires and interviews. Based on the results of the Mann-Whitney difference test and Independent Sample T-test, there were significant differences (p<0.05) in the online activity time variable (p=0.041), energy adequacy level (p=0.040), frequency of consumption of staple food (p=0.004), protein vegetable (p=0.021), and drinks (p=0.003) between normal and overweight subjects. The results of the Spearman correlation test showed that there was a significant positive relationship (p<0.05) between the level of adequacy of energy, protein, fat, carbohydrates, and vitamin C with the quality of food consumption. Gender variable has a significant relationship (p<0.05) with stress levels. Based on the Spearman correlation test, there is no significant relationship (p>0.05) between stress levels and the quality of food consumption with nutritional status.
Keyword: adolescents, level of nutritional adequacy, nutritional status, quality of food consumption, stress level

Judul: Isolasi Konstruksi dan Rekombinasi Gen Pengkode Reverse Transcriptase asal Simian Retrovirus-2 (SRV-2)
Abstrak: The availability of reverse transcriptase enzym as a valuable component for Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique has been providing by foreign companies. Therefore, it needs more cost and time to provide it. This study is an early step to produce in house reverse transcriptase rekombinant enzym from SRV-2. The purpose of this study was isolated, constructed, and rekombined SRV-2 reverse transcriptase gene to entry clone and destination vector. Isolation was done by PCR amplification technique using three pairs of primers, which are RT I (3282-3683), RTII (3261-3900), and RTIII (3169-4133). Construction and recombination in this study used Gateway method from Invitrogen. SRV-2 reverse transcriptase gene was isolated in this study with A-549 cell inoculated SRV-2 as source of virus. This gene amplify by PCR technique and resulted specific band for RTI 401 bp, RTII 639 bp, and RTIII 964 bp. Analysis of pENTR/SD/TOPO and pDEST17 indicated SRV-2 reverse transcriptase gene code has already inserted to this plasmid. This result indicated SRV-2 reverse transcriptase gene have isolated, constructed, and recombined to entry clone and destination vector.
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