Judul: Pengembangan Backend Forum Tani pada Digital Tani dengan Metode Prototyping
Abstrak: Indonesia adalah negara agraris yang sebagian besar masyarakatnya bermata pencaharian sebagai petani. Kurangnya edukasi yang merata mengenai pertanian mengakibatkan sulitnya pemahaman tentang permasalahan baru yang dihadapi petani. Adanya forum online dapat membantu petani dalam mendiskusi permasalahan baru dengan lingkup yang lebih luas. Tani Center IPB mengembangkan aplikasi Digital Tani sebagai one stop solution untuk masalah pertanian Indonesia. Penelitian ini bertujuan untuk mengembangkan backend forum tani pada aplikasi Digital Tani dengan metode prototyping. Sistem dikembangkan dengan menggunakan framework Laravel dengan arsitektur Model, View, Controller (MVC). Hasil pengembangan diuji menggunakan blackbox testing dan telah berhasil memenuhi Minimum Viable Product (MVP) dengan iterasi yang dilakukan sebanyak 2 kali., Indonesia is an agricultural country where most of the people make a living as farmers. The lack of equitable education about agriculture makes it difficult to understand the new problems faced by farmers. The existence of online forums can help farmers in discussing new problems with a wider scope. Tani Center IPB developed the Digital Tani application as a one stop solution for Indonesian agricultural problems. This study aims to develop a farmer forum backend on the Digital Tani application using the prototyping method. The system was developed using the Laravel framework with Model, View, Controller (MVC) architecture. The results of the development were tested using blackbox testing and had successfully met the Minimum Viable Product (MVP) with 2 iterations.
Keyword: agriculture, backend, forum, MVC, prototyping method

Judul: Pengembangan Backend Pengelolaan Artikel untuk Aplikasi Digital Tani
Abstrak: IPB sebagai kampus yang terus mendukung pertanian Indonesia telah melakukan banyak inovasi sebagai upaya untuk memajukan pertanian Indonesia. Salah satu bentuk upayanya dengan mengembangkan aplikasi Digital Tani. Pada aplikasi digitani terdapat fitur artikel untuk memudahkan petani dalam mendapatkan informasi. Saat ini pembuatan artikel dilakukan di Wordpress dan aplikasi digitani berjalan di arsitektur yang berbeda. Penelitian ini akan mengembangkan backend pengelolaan artikel untuk mengintegrasikan artikel yang ada pada Wordpress dengan aplikasi Digitani. Pengembangan menggunakan metode prototyping dan hasil dari penelitian ini adalah backend berbasis REST API dengan response yang dihasilkan adalah JSON. Pengujian dilakukan dengan internal testing menggunakan metode blackbox dan semua fungsi berhasil berjalan dengan sesuai sehingga dapat dikatakan pengembangan berhasil dilakukan., IPB as a campus that continues to support Indonesian agriculture has made many innovations as an effort to advance Indonesian agriculture. One of the efforts is to develop the Digital Tani application. In the Digitani application there is an article feature to help farmers in obtain information. Currently, the creation of articles is done on Wordpress and the Digitani application runs on a different architecture. This study will develop an article management backend to integrate existing articles on Wordpress with the Digitani application. The development uses the prototyping method and the result of this research is a REST API-based backend with the resulting response is JSON. Testing was carried out by internal testing using the blackbox method and all functions were running successfully so that it can be said that the development was successful.
Keyword: Backend, Digital Tani, Prototyping, Wordpress

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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