Judul: Characteristic of Nucleotide Sequence of COI Gene of Blue Swimming Crab Portunus pelagicus (Linnaeus, 1758) for Fisheries Management in WPP-NRI 711
Abstrak: Sumberdaya perikanan Indonesia yang bersifat multispesies dapat menimbulkan fenomena cryptic dan complex species, sehingga dibutuhkan metode yang akurat dalam pemastian spesies rajungan, yaitu dengan DNA barcoding. Penelitian ini bertujuan menentukan kepastian spesies dan menganalisis kondisi populasi rajungan (Portunus pelagicus) di perairan WPP-NRI 711 berdasarkan gen COI. DNA contoh rajungan berjumlah 10 individu yang didapatkan dari tiga lokasi, yaitu perairan Bintan, Bangka, dan Ketapang telah diisolasi. Kualitas untaian DNA yang baik digandakan dan diurutkan pada proses sekuensing. Seluruh contoh rajungan tervalidasi secara genetik sebagai spesies Portunus pelagicus. Komposisi basa nukleotida dari contoh rajungan didominasi oleh pasangan basa adenin-timin. Keragaman genetik rajungan di perairan Ketapang dan Bintan tergolong tinggi, sedangkan di perairan Bangka tergolong sedang. Ketiga lokasi penelitian memiliki nilai Fst yang tergolong rendah. Hasil penelitian menunjukkan bahwa populasi rajungan berada pada kondisi yang baik., Indonesia's multi-species fisheries resources can cause cryptic and complex species phenomena, so an accurate method is needed to determine the blue swimming crab species, such as DNA barcoding. This study aimed to determine the species certainty and to analyze the population status of blue swimming crab (Portunus pelagicus) based on the COI gene in WPP-NRI 711. A total of 10 blue swimming crab samples DNA obtained from three locations such as Bintan, Bangka, and Ketapang waters was isolated. High quality DNA strands are duplicated and sequenced. All of the samples have been genetically validated as Portunus pelagicus species. The nucleotide base composition of the COI gene from samples was dominated by adenine-thymine pairs. The genetic diversity value in Ketapang and Bintan are high, while in Bangka is moderate. All sampling sites have a low fixation index value. It can be concluded that the blue swimming crab populations are in a good condition.
Keyword: blue swimming crab, COI gene, molecular analysis

Judul: Keragaman genetik rajungan Portunus pelagicus (Linnaeus, 1758) di WPP NRI 572 dan 573 berdasarkan marka gen COI.
Abstrak: Rajungan merupakan anggota Crustacea yang memiliki nilai ekonomi dan peminat yang tinggi pada pasar lokal maupun ekspor. Hal tersebut menjadikan rajungan banyak ditangkap di perairan Indonesia. Tingginya Intensitas penangkapan rajungan tanpa adanya pengelolaan dapat mengakibatkan menurunnya keragaman genetik. Penelitian ini bertujuan untuk menganalisis kekerabatan dan keragam genetik rajungan yang tertangkap di Wilayah Pengelolaan Perikanan Negara Republik Indonesia (WPP-NRI) 572 (Labuan) dan WPP-NRI 573 (Cilacap-Banyuwangi) menggunakan marka gen COI sebagai dasar pengelolaan sumberdaya rajungan. Sebanyak 90 contoh rajungan dari lokasi penelitian di ambil 30 individu untuk di analisis molekuler. Seluruh contoh rajungan yang dianalisis memiliki nilai persen identifikasi sebesar 99-100% sebagai Portunus pelagicus. Nilai jarak genetik antarsampel menunjukan kekerabatan yang dekat karena memiliki nilai dibawah 3%. Pohon filogeni menunjukan hasil kekerabatan rajungan dari tiap lokasi sangat dekat serta memiliki keragaman genetik yang tergolong sedang dan tinggi. Upaya pengelolaan yang dapat dilakukan yaitu mempertahankan tingkat eksploitasi dan evaluasi dengan meningkatkan monitoring pada WPP-NRI 573, sedangkan pada WPP-NRI 572 dilakukan penurunan intensitas penangkapan., Blue swimming crab (BSC) is a member of Crustacea which has high economic value and interest in local and export markets. Because of those reasons, blue swimming crabs are caught in Indonesian waters. The high intensity of crab fishing without management can decreased the genetic diversity. This study aims to analyze the kinship and genetic diversity of blue swimming crabs in the Fisheries Management Areas of the Republic of Indonesia ( FMA-RI) 572 (Labuan) and FMA-RI 573 (Cilacap-Banyuwangi) using COI gene markers as the basis for blue swimming crab resource management. A total of 30 individuals out of 90 samples taken were used for molecular analysis. All samples of BSC analyzed had a percent identification value of 99-100% as Portunus pelagicus. The value of the genetic distance between samples shows close kinship because it has a value below 3%. The phylogenetic tree shows the results of blue crab kinship from each location which are very close and have moderate and high genetic diversity. Management efforts can be carried out to maintain the level of exploitation and evaluation by increasing monitoring at FMA-RI 573, while at FMA-RI 572 a reduction in fishing intensity is carried out.
Keyword: Blue swimming crab, COI, genetic distance, genetic diversity

Judul: Pembuatan Film Tipis Cu2ZnSnS4 sebagai Lapisan Penyerap Cahaya pada Sel Surya Menggunakan Metode Elektrodeposisi
Abstrak: Thin film of Cu2ZnSnS4 (CZTS) is an alternative semiconductor used as light absorber layer on solar cell. CZTS can replace silicon that commonly used as commercial semiconductor. The advantage of using CZTS as absorber layer is due to the abundance of its chemical composer on earth, and its high value of absorption coefficient. The absorber layer was made by using electrodeposition method on ITO glass substrates. The films were deposited at room temperature using potentiostatic mode with a potential of –1.05 V for 30, 45, and 60 minutes. After that, the films were annealed at two different temperatures, 180 ºC and 500 °C, for 45 minutes in N2/H2S atmosphere. Based on X-ray diffraction analysis, the polycrystalline thin film obtained fall into kesterite crystal phase. This type of crystal was obtained when the CZTS was annealed at 500 °C in H2S/N2 atmosphere. The value of optical band gap energy for the CZTS thin film was 2.2 eV with the film thickness about 1.228–6.366 μm. The sample of CZTS with electrodeposition time of 45 minutes and annealing temperature of 500 °C showed the best result and in conformity with absorber layers criteria for solar cells.
Keyword: semiconductor, ITO glass, CZTS thin film, electrodeposition