Judul: Keragaman gen Toll-Like Receptor4 pada ayam lokal dan persilangan kampung - broiler
Abstrak: Ayam kampung merupakan salah satu ayam lokal Indonesia yang telah terkenal dengan kelezatan dan ketahanannya terhadap penyakit, terutama tahan terhadap Salmonella. Salah satu penciri genetik ketahanan ayam pada bakteri ini adalah gen Toll-like Receptor 4 (TLR4). TLR4 (toll-like receptor 4) merupakan gen pengkode protein TLR4 yang mengontrol sinyal imun non spesifik sebagai pertahanan pertama terhadap serangan bakteri S. entiritidis. Tujuan penelitian ini adalah mengidentifikasi keragaman gen TLR4 pada ayam lokal Indonesia dan persilangannya (kampung, broiler, KB, KBKB, BK, kapas, sentul, dan merawang). Gen TLR4 berhasil digenotyping pada 214 ayam dengan teknik PIRA-PCR. Genotyping dilakukan pada exon 2 dengan panjang produk 220 bp. Enzim restriksi MscI memotong produk PCR dengan panjang fragmen 196 bp dan 24 bp, diperoleh dua alel (G dan A), dan tiga genotipe (GG, AG, dan AA). Hasil penelitian menunjukkan bahwa ayam KBKB, KB, kampung, merawang, dan sentul bersifat polimorfik (0.05-0.33). Ayam broiler, BK, dan kapas bersifat monomorfik. Keragaman tertinggi ditunjukkan oleh rumpun ayam lokal yaitu kampung, merawang, dan sentul. Nilai PIC tertinggi berada pada ayam lokal (ayam kampung) dengan kategori sedang (0.40). Kata kunci : ayam lokal, genotyping, keragaman, TLR4
Keyword: Genotyping, Local chicken, Polymorphism, TLR4, Salmonella e., Protein coding gene, Immue system, Monomerphics, Heterozygosity

Judul: Keragaman Gen Toll-like Receptor 4 (TLR4) dan Myxovirus (Mx) pada Ayam Kampung serta Asosiasinya terhadap Sifat Pertumbuhan.
Abstrak: Kampung chicken has two production purposes as meat and egg producer. As layer chicken, the egg of kampung chicken was used as jamu. So, the food security of kampung chicken egg needs to be guaranted especially from Salmonella sp. The other problem which commonly happened in kampung chicken industry was the highest of mortality rate which caused by Newcastle Disease (ND) virus infection. Genetically, there were some gene that control self endurance against Salmonella sp and virus infection such as TLR4 and Mx genes. Research aimed to identify the polymorphisms of TLR4 and Mx genes in kampung chicken and the association with growth traits. Research consisted of two steps. The first step was analysis of TLR4 and MX genes polymorphisms and the second step was the association of TLR4 and Mx genes with growth traits. A total of 98 bird of kampung chickens were used in the first procedure and 45 birds were used in the second procedure. Identification of TLR4 and Mx genes polymorphisms was done using PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) method. Data analysis were genotype frquency, allele frequency, heterozygosity, Hardy- Weinberg equilibrium, Polymorphics Informative Content value and association between genotype and growth used GLM (General Linear Model). The result showed GA mutation on TLR4 gene and AG mutation on Mx gene. TLR4 and Mx gene were polymorphics. There was no association between TLR4 gene and growth traits in kampung chicken. Kampung chicken which resist to Salmonella sp and ND virus had low 16 weeks body weight, total average gain daily, and average weekly gain.
Keyword: growth traits, kampung chicken, Mx gene, TLR4 gene

Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4