Judul: Genetic authentication on morphological variation in cultivated pompano (Trachinotus spp.) based on the 16s rRNA gene marker
Abstrak: Ikan bawal (Trachinotus spp.) termasuk komoditas unggulan perairan laut dangkal yang mengalami peningkatan permintaan sehingga perlu dilakukan konservasi melalui kegiatan budidaya. Penelitian ini bertujuan mengautentikasi spesies ikan bawal yang dibudidayakan di BPBL Batam sebagai informasi keragaman genetik dan kekerabatannya. Penelitian dilakukan pada bulan April hingga November 2021. Metode yang digunakan yaitu isolasi dan ekstraksi DNA, amplifikasi dan visualisasi, dan sekuensing gen 16s rRNA. Analisis data meliputi analisis morfologi, pensejajaran, analisis filogeni dan jarak genetik menggunakan MEGA 11. Seluruh sampel menghasilkan DNA total yang baik namun terdapat kontaminan. Hasil PCR menunjukkan pita DNA tunggal dan tebal dengan ukuran basa sesuai target. Hasil validasi menunjukkan seluruh sampel merujuk pada T. blochii dengan nilai persen identifikasi 100% dan nilai jarak genetik 0,000. Pohon filogeni menunjukkan tiga spesies bawal berada satu klaster dengan famili Carangidae. Oleh karena itu, ketiga jenis bawal memiliki kemiripan yang cukup identik dengan spesies T. blochii., Pompano (Trachinotus spp.) is one of the leading commodities in shallow marine waters which is experiencing an increase in demand, so it is necessary to conserve it through aquaculture. This study aims to authentify species of pompano cultivated at BPBL Batam as information on genetic diversity and kinship. The study was conducted from April to November 2021. The methods are using DNA isolation and extraction, amplification and visualization, and 16s rRNA gene sequencing. Data analysis includes morphological analysis, alignment, phylogeny analysis, and genetic distance using MEGA 11. All samples produced good total DNA but contained contaminants. The result of PCR showed DNA single and thick with the base size according to the target. Results of validation showed that all samples referred to T. blochii with a percent identification value of 100% and a genetic distance is 0.000. The phylogeny tree shows that three types of pompano are in one cluster with the family of Carangidae. Therefore it can be concluded that three types of pompano have an identical resemblance to the species of T. blochii.
Keyword: 16s rRNA, genetic, pompano, T. Blochii

Judul: Species Identification in Fisheries Products using Genetic Molecular Marker Cytochrome B Minibarcode
Abstrak: Fish is one of the largest food commodities, so market demand for fish is high. Fish with high economic value is one of the high market demands, this causes fraud in the sale of fish. DNA barcoding is used to identify fishery processed products as well as the authenticity of raw materials so as to reduce food safety problems. The isolation method used was Cetyltrymethyl Ammonium Bromide (CTAB) with concentration and purity testing using a Nanodrop spectrophotometer. The 25 samples consisting of frozen fish fillets, processed fishery product, and salted fish fillets from traditional and supermarket in Jabodetabek. DNA isolates have a concentration of 4,65-3172,6 ng/μL, with a purity of 1,067-2,125. An electrophoregram showed 7 samples sampled on a target DNA of 200-300bp. Analysis using BLAST showed samples detected as Makaira nigricans, Rachycentron canadum, Lates calcarifer, Priacanthus tayenus, Lepidocybium flavobrunneum, and Salmo salar. Phylogeny analysis showed the samples formed a group corresponding to database from Genbank., Ikan menjadi salah satu komoditi pangan yang cukup besar, sehingga permintaan pasar terhadap ikan menjadi tinggi. Ikan dengan nilai ekonomis tinggi menjadi salah satu permintaan pasar yang tinggi, hal tersebut menyebabkan terjadinya kecurangan dalam penjualan ikan. DNA barcoding dengan penanda genetik cytochrome b digunakan untuk mengidentifikasi produk olahan perikanan serta keaslian bahan baku sehingga dapat mengurangi permasalahan keamanan pangan. Metode isolasi yang digunakan adalah Cetyl Trymethyl Ammonium Bromide (CTAB) dengan pengujian konsentrasi dan kemurnian menggunakan spektrofotometer Nanodrop. Sampel berjumlah 25 terdiri dari filet ikan beku, produk olahan perikanan, dan filet ikan asin yang didapat dari pasar tradisional dan swalayan di Jabodetabek. Isolat DNA memiliki konsentrasi 4,65-3172,6 ng/µL, dengan kemurnian 1,067-2,125. Elektroforegram menunjukan 7 sampel teramplifikasi pada DNA target 200-300bp. Hasil analisis menggunakan BLAST menunjukan sampel terdeteksi sebagai Makaira nigricans, Rachycentron canadum, Lates calcarifer, Priacanthus tayenus, Lepidocybium flavobrunneum, dan Salmo salar. Analisis filogeni menunjukan sampel membentuk kelompok yang sesuai dengan basis data dari Genbank.
Keyword: Cytochrome b, DNA barcoding, fishery products, PCR

Judul: Pembuatan Film Tipis Cu2ZnSnS4 sebagai Lapisan Penyerap Cahaya pada Sel Surya Menggunakan Metode Elektrodeposisi
Abstrak: Thin film of Cu2ZnSnS4 (CZTS) is an alternative semiconductor used as light absorber layer on solar cell. CZTS can replace silicon that commonly used as commercial semiconductor. The advantage of using CZTS as absorber layer is due to the abundance of its chemical composer on earth, and its high value of absorption coefficient. The absorber layer was made by using electrodeposition method on ITO glass substrates. The films were deposited at room temperature using potentiostatic mode with a potential of –1.05 V for 30, 45, and 60 minutes. After that, the films were annealed at two different temperatures, 180 ºC and 500 °C, for 45 minutes in N2/H2S atmosphere. Based on X-ray diffraction analysis, the polycrystalline thin film obtained fall into kesterite crystal phase. This type of crystal was obtained when the CZTS was annealed at 500 °C in H2S/N2 atmosphere. The value of optical band gap energy for the CZTS thin film was 2.2 eV with the film thickness about 1.228–6.366 μm. The sample of CZTS with electrodeposition time of 45 minutes and annealing temperature of 500 °C showed the best result and in conformity with absorber layers criteria for solar cells.
Keyword: semiconductor, ITO glass, CZTS thin film, electrodeposition