Judul: Pengukuran Hambur Balik Akustik Dasar Laut di Sekitar Kepulauan Seribu Menggunakan Split Beam Echosounder.
Abstrak: The purpose of this research is to compute the backscattering strength of the seabed by measuring volume backscattering strength (SV), bottom surface backscattering strength (SS), and the echo level (EL) from seabed using split beam echosounder. The research was conducted from 29th January to 3rd February 2011, around the Seribu Islands: Pramuka island, Panggang island, Karya island and Semak Daun island, North Jakarta. Acquisition of acoustic data was conducted using the SIMRAD EY 60 instrument. Acoustic data obtained from 9 stations simultaneously with sediment sampling. Acoustic processing data was conducted by Rick Towler program with Matlab based. The SV and SS were analyzed Manik et al, model by using. Sediment sampling station consisted of 9 stations: Pramuka island there are 1 station (Station 1), Karya island there are 2 stations (Station 2, and Station 3), Panggang island there are 3 stations (Station 4, Station 7, Station 9), and Semak Daun island there are 3 stations (Station 5, Station 6, Station 8). Sediment classified based on the sediment texture. Seabed surface sediments were separated into 3 types, they are: sand, mud, and clay. This analysis showed that the location of the 9 stations observation is dominated by sand fraction with the percentage of 80.85%. Mud and clay fractions had the average percentage value of 18.32% and 0.83%, respectively. The backscattering value (SV) of sand substrate ranged -10.62 to -18.51 dB with the average of -13.91 dB, and the muddy sand substrate ranged from -16.58 to -25.42 dB with the average -20.57 dB. The value of SS for the sand substrate ranged from -20.70 to -28.58 dB with the average value of -23.98 dB. Muddy sand substrate has a value of SS in the range of -26.64 to -35.49 dB with the average SS of -30.64 dB, from this research, the classification of seabed type using hydroacoustic technology was possible.
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Judul: Measurement of acoustic backscattering strength of sea-bottom with single and multi beam echosounder
Abstrak: Underwater acoustic instruments has been an indispensable tool to study the ocean. Application of acoustic such as sonar technologies to bottom acoustics study has made significant advances over recent decades. The sonar systems evolved from single beam systems to more sophisticated digital ones like multi beam echo sounder. In this paper, both systems of single beam echo sounder (SBES) and multi beam echo sounder (MBES) were applied to detect and measure acoustic backscattering strength of sea-bottom. Sampling sediment was taken for ground truth data using bottom sampler. For SBES, data collection was carried out in Seribu Islands, Jakarta. Data were collected using CruzPro PcFF80 FishFinder with operating frequency 200 kHz. The algorithm to compute underwater signal using this instrument had been developed by Manik (2011). For MBES, data collection was carried out in Buyat Bay, North Sulawesi Province. Data were collected using Elac Seabeam 1050D MBES with operating frequency 180 kHz and processed with MBSystem version 5 software. For SBES, the results showed the value of surface backscattering strength (SS) for sand varied from -18.68 dB to -17.39 dB, silty sand -23.25 dB to -19.32 dB, and clayey sand was -19.83 dB. The echo level (EL) for sand varied from 131.4 dB to 138.2 dB, silty sand 133.5 dB to 135.4 dB, and for clayey sand was 136.5 dB (using source level (SL) 163 dB). For MBES, the SS for silty sand varied from -24.26 dB to -20.56 dB, and sandy silt -25.12 dB to -24.30 dB.
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Judul: Gene Editing  of Two Cyanide Synthesis-related Genes in Cassava using CRISPR/Cas9
Abstrak: Cassava (Manihot esculenta) contain toxic quantities of cyanogenic glucoside. Reducing the potentially toxic cyanogenic glucoside levels in cassava could be achieved by gene editing through Clustered Regularly Interspaced Short Palindromic Repeats – CRISPR associated protein 9 (CRISPR/Cas9), a high- precision genome editing technique in plants. Two cassava UDP- glucosyltransferase (UGT) paralogs, UGT85K4 and UGT85K5, are the genes catalyzing the synthesis of cyanogenic glucosides in plants. The study aimed to construct the CRISPR/Cas9 recombinant vector carrying single guide RNA (sgRNA) to edit UGT85K4 and UGT85K5 genes, and transform sgRNA CRISPR/Cas9 construct into KU50 cassava calli. Five sgRNAs were designed, considering the on/off target efficiency score and GC content. Those sgRNAs were ligated into pRGEB32 plasmid and introduced to competent Escherichia coli DH5a. The PCR colony and DNA sequencing were performed to verify the sgRNA insert. The amplicons showed the expected band size of recombinant vector (180 bp). Based on sequencing results, only one sgRNA was successfully ligated into the plasmid that will be used to edit the UGT85K5 gene. The sgRNA CRISPR/Cas9 construct in pRGEB32 plasmid was successfully transformed into Agrobacterium tumefaciens and introduced into KU50 cassava calli through co-cultivation. The overgrowth of Agrobacterium occurred after the co-cultivation phase.
Keyword: CRISPR/Cas9, Cyanogenic glucoside, Gene editing, sgRNA