Judul: Analisis Molekuler dan Fenotipe Galur Padi Transgenik IR64 Generasi T3 yang Mengandung Penanda Aktivasi untuk Toleransi Kekeringan
Abstrak: Activation tagging transgenic rice cv. IR64 had been developed by introducing a construct of activation tagging transposon Ac/Ds. Phenotipic character associated with drought stress tolerance of the transgenic rice lines have not been analyzed. This study aimed to evaluate T3 generation of activation tagging transgenic rice lines cv. IR64 to drought stress treatment. The methodology of study consisted of several steps including germination of samples seeds, screening the seedling with drought strees treatment, molecular analysis of the rice lines identify hpt and bar genes, and observation of agronomic character. Results of study showed that there were 34 out of 84 plants that tolerant to drought stress. A total of 23 plants of tolerant plants were detected carrying only bar gene after PCR analysis and indicated that the plants were stable mutants. Generaly, compared to the non-transgenic plants, there were changes in several agronomic parameters of transgenic rice plants cv. IR64 T3 generation. A total of nine plants increased of its height, six plants had a faster harvesting time, and 21 plants had higher weight of 100 seeds compared by non-transgenic plants.
Keyword: Bogor Agricultural University (IPB), drought stress tolerance, activation tagged, Rice (Oryza sativa L.)

Judul: Over-expression of OsDREB1A gene for improvement of drought tolerance on rice.
Abstrak: Drought is a major factor that influence the growth and productivity of rice. Drought tolerant rice varieties are needed to increase rice production. Development of improved rice varieties can be done through classical breeding and genetic transformation. The research objective was to obtain rice plants containing the construct 35S::OsDREB1A. Agrobacterium tumefaciens mediated transformation of rice cv. Nipponbare using OsDREB1A gene under the control of CAMV 35 promoter produced 81 putative transgenic rice plants (T0) positively containing the transgene based on PCR analysis. The plant height, panicle number, panicle length, and the grain number of transgenic plants were in general lower than the Nipponbare wild type. T1 seeds from selected genotypes were screened for hygromycine resistence, and rice seedlings were evaluated for tolerance to drought stress in the glasshouse, based on the standard evaluation system for rice (IRRI 1996). Five independent transgenic rice plants were identified to be tolerant to drought stress. Molecular characterization using Southern hybridization showed that these plants had 2- 4 copies of transgene. Physiological analysis showed that the drought avoidance is the possible mechanism of adaptation to drought of the transgenic plants. The drought tolerant transgenic plants were crossed to Indonesian elite rice cultivars (Ciherang, Batutegi, Code, and Konawe). Glasshouse evaluation showed that F1 transgenic plants derived from cultivars Batutegi, Code, and Konawe were more tolerant to drought than their wild-type counterparts. F1 transgenic plants were backcrossed into corresponding elit rice cultivars, and PCR analysis showed that those plants contained the transgene. These results showed that 35S::OsDREB1A could be transferred and inherited to elite rice genotipes through crossing. The new drought tolerant germplasms generated in this research can be useful materials for breeding program for improvement of drought tolerance on rice in Indonesia.
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Judul: Karakteristik Anatomi Skelet Kepala Buaya senyulong (Tomistoma schlegelii).
Abstrak: Buaya sepit atau senyulong (Tomistoma schlegelii) merupakan jenis buaya asli Indonesia yang berhabitat di sungai-sungai Sumatera, Kalimantan, dan juga pedalaman Sulawesi. Salah satu ciri khas buaya senyulong adalah bentuk moncong yang runcing dan sempit, namun hingga saat ini informasi mengenai struktur skelet kepala buaya senyulong sangat terbatas. Penelitian ini bertujuan untuk mempelajari karakteristik anatomi skelet kepala buaya senyulong yang dikatikan dengan fungsi dan perilakunya. Penelitian dilakukan secara eksploratif dengan melakukan pengamatan, pencatatan, dan pengambilan gambar dari skelet kepala buaya senyulong. Skelet kepala berukuran panjang 70 cm, lebar 29 cm, dan tingggi 17 cm dengan permukaan kasar pada os nasale, os frontale, os maxilla, os mandibula, os lacrimale, os jugale. Jumlah gigi pada buaya ini sebanyak 78 buah, terdiri dari 4 buah gigi premaxila, 16 buah gigi maxila, dan 19 buah gigi mandibula. Pada regio orbitalis ditemukan dua pasang fenestrae temporales yang berkembang baik. Fenestra supratemporale merupakan lubang oval yang membentang di sepanjang os parietale. Fenestra infratemporale berbentuk segitiga dan dibatasi oleh os squamosum, os postorbitale, os jugulare, dan os quadratum. Os mandibula hewan ini mempunyai symphysis mandibularis yang panjang yaitu garis yang melintang di corpus mandibulae. Secara fungsional ukuran symphysis mandibularis berkaitan dengan adaptasi hewan terhadap diet makan.
Keyword: kepala, moncong, Tomistoma schlegelii