Judul: Induksi Kalus dan Embrio Somatik Tanaman Jambu Biji Merah (Psidium guajava L.)
Abstrak: The objective of the research was to get the best protocol for callus and somatic embryo induction of guava. The experiment was conducted at Tissue Culture Laboratory, Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University from September 2012 until March 2013. The research was arranged into two experiments, that were callus induction and somatic embryo induction. The results showed that media with plant growth regulator composition treatments R2 (MS + 5 mg l-1 2.4-D), R4 (MS + 5 mg l-1 2.4-D + 1 mg l-1 BAP) and R5 (MS + 2.5 mg l-1 2.4-D + 2 mg l-1 2-iP) were the best treatments for inducting compact callus in 4 weeks after culture (WAC). In while, the friable callus can be induced at media with plant growth regulator composition treatment R6 (MS + 5 mg l-1 2.4-D + 2 mg l-1 2-iP). The primary somatic embryos can be induced at the treatment KR1E4 (callus from MS + 2.5 mg l-1 2.4-D was moved into MS + 1 mg l-1 2.4-D + 2 mg l-1 2-iP) and the treatment KR6E4 (callus from MS + 5 mg l-1 2.4-D + 2 mg l-1 2-iP was moved into MS + 1 mg l-1 2.4-D + 2 mg l-1 2-iP) in 12 and 16 WAC for each treatment.
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Judul: Induksi Embrio Somatik Melon (Cucumis melo L.) pada Beberapa Media yang Dilengkapi dengan Auksin dan Sitokinin
Abstrak: Penelitian ini terdiri dari dua percobaan. Percobaan I bertujuan untuk mengetahui jenis media dan konsentrasi zat pengatur tumbuh picloram (auksin) yang terbaik dalam induksi embriogenesis somatik pada melon menggunakan eksplan biji muda. Sedangkan percobaan II bertujuan untuk mengetahui konsentrasi beberapa jenis auksin (2.4 D dan NAA) dengan BAP (sitokinin) yang terbaik untuk menginduksi embriogenesis somatik pada melon menggunakan eksplan hipokotil. Penelitian dilaksanakan di Laboratorium Kultur Jaringan Departemen Agronomi dan Hortikultura Institut Pertanian Bogor pada bulan April-September 2009. Percobaan I dilaksanakan dengan menggunakan Rancangan faktorial yang disusun dalam Rancangan Acak Kelompok (RAK). Penelitian terdiri dari dua faktor dengan faktor pertama media tanam terdiri dari tiga taraf media yaitu MS (Murashige and Skoog), WPM (Woody Plant Medium), dan B5. Konsentrasi picloram sebagai faktor kedua terdiri dari empat taraf yaitu 0, 0.5, 1.0, 1.5 mg/L. Percobaan dilakukan dengan 3 ulangan dan setiap ulangan terdapat 5 botol. Setiap botol terdapat 5 eksplan. Sumber eksplan yang digunakan yaitu biji muda melon hibrida H7 koleksi Pusat Kajian Buah-Buahan Tropika IPB yang berumur ± 15 Hari Setelah Penyerbukan.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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