Judul: Pengaruh dukungan sosial dan interaksi keluarga terhadap kebahagiaan keluarga generasi sandwich
Abstrak: Generasi sandwich di Indonesia, yang tinggal bersama dalam keluarga besar dengan kakek, nenek, atau saudara kandung orang tua, sering menghadapi stres dan penurunan kebahagiaan karena tanggung jawab ganda dalam memenuhi kebutuhan diri sendiri dan orang lain. Dukungan sosial dari keluarga melalui interaksi yang baik dapat membantu mengurangi stres dan memperkuat ketahanan keluarga.Tujuan dari penelitian ini adalah untuk menganalisis pengaruh karakteristik keluarga, dukungan sosial, interaksi keluarga, terhadap kebahagiaan keluarga. Pengambilan contoh menggunakan teknik non-probability sampling dengan melibatkan 40 istri dari keluarga generasi sandwich di Kelurahan Situgede, Kecamatan Bogor Tengah, Kota Bogor, Jawa Barat. Pengambilan data dilakukan pada bulan Januari-Maret 2024. Hasil uji korelasi menunjukkan bahwa terdapat hubungan signifikan positif antara pendapatan keluarga dengan variabel interaksi keluarga dan dukungan sosial. Hasil uji regresi menunjukan bahwa terdapat pengaruh signifikan positif antara jumlah anak dan usia istri terhadap kebahagiaan keluarga. Selain itu, variabel interaksi keluarga berpengaruh signifikan positif terhadap kebahagiaan keluarga. Tidak terdapat hubungan signifikan antara variabel dukungan sosial dengan kebahagiaan keluarga, The sandwich generation in Indonesia, who live together in extended families with grandparents or siblings, often face stress and decreased happiness. parents' siblings, often face stress and decreased happiness due to the dual responsibilities of meeting the needs of themselves and others. others. Social support from family through good interactions can help reduce stress and strengthen family resilience. The purpose of this study is to analyze the effect of family characteristics, social support from the family through good interactions. The purpose of this study is to analyze the effect of family characteristics, social support, family interaction, on family happiness. Sampling using non-probability sampling technique involving 40 wives of sandwich generation families in from a sandwich generation family in Situ Gede Village, Bogor Central, Bogor City, West Java. Data collection was conducted in January-March 2024. The correlation test results show that there is a positive between family income and the variables of family interaction and social support. and social support. The regression test results show that there is significant positive effect between the number of children and wife's age on family happiness. family happiness. In addition, the family interaction variable has a significant positive effect on family happiness. There is no significant relationship between social support variables and family happiness
Keyword: family interaction, happiness, sandwich generation, social support

Judul: The Influence of Gender Roles and Husband-Wife Interaction among Sandwich Generation Family's Happiness
Abstrak: Seseorang yang telah menikah akan tinggal bersama dengan keluarga intinya, namun terdapat rumah tangga yang harus mengasuh orang tua dan anak secara bersamaan sehingga dikategorikan dalam generasi sandwich. Tujuan penelitian ini untuk menganalisis peran gender, interaksi suami-istri, dan kebahagiaan keluarga generasi sandwich. Pengambilan contoh menggunakan teknik non-probability purposive sampling dengan melibatkan 40 istri dari keluarga generasi sandwich di Kecamatan Cicendo, Astana Anyar, dan Bandung Kulon yang merupakan kecamatan wilayah Kota Bandung. Pengambilan data dilakukan pada bulan April-Mei 2023. Data diolah menggunakan analisis deskriptif, uji korelasi Pearson, dan uji regresi linear berganda. Hasil menunjukkan bahwa peran gender berada pada kategori rendah, sedangkan interaksi suami-istri dan kebahagiaan keluarga berada pada kategori tinggi. Uji korelasi menunjukkan terdapat hubungan signifikan positif antarvariabel. Uji regresi menunjukkan bahwa terdapat pengaruh antara jumlah lansia dan kontribusi lansia dalam pemenuhan ekonomi terhadap kebahagiaan keluarga generasi sandwich. Dimensi kohesi pada variabel interaksi suami-istri juga memiliki pengaruh signifikan positif terhadap kebahagian keluarga generasi sandwich. Artinya, ikatan emosional antaranggota keluarga memiliki pengaruh yang signifikan terhadap kebahagiaan keluarga., A married individual shall reside with their nuclear family, however, there are households that must care for both parents and children simultaneously, and categorized as the sandwich generation. The purpose of this study was to analyze the role of gender, spousal interaction, and family happiness in the sandwich generation. Sampling was conducted using the non-probability purposive sampling, involving 40 wives from sandwich generation families in the Cicendo, Astana Anyar, and Bandung Kulon districtss in Bandung City. Data collection took place in the months of April-May 2023. The data was processed using descriptive, Pearson correlation, and multiple linear regression analysis. The results indicate that the role of gender in the low category, while spousal interaction and family happiness in high category. The correlation test shows a significant positive relationship between the variables. The regression analysis shows that there was an influence of the number of elderly individuals and their contribution to economic fulfillment on the happiness of the sandwich generation family. The dimension of cohesion in the variable of spousal interaction also has a significant positive impact on the happiness of the sandwich generation family. This means that the emotional bonds among family members have a significant influence on family happiness.
Keyword: gender, generasi sandwich, interaksi, kebahagiaan keluarga

Judul: Pemurnian Rekombinan Eritropoietin Manusia (rhEPO) Menggunakan Kromatografi Afinitas Ni-NTA dan Co-Talon
Abstrak: Suitable and efficient techniques for purification of recombinant human erythropoietin (rhEPO) are required to obtain pure rhEPO which can be continued to preclinical trials step. One of these techniques is immobilized metal affinity chromatography (IMAC), which has advantages such as high selectivity, easy to elute, and simple procedure for column regeneration. At least, there are 2 kinds of commercial IMAC resin using nitriloacetic acid (NTA) matrix as chelating agent, namely nickel-based (Ni-NTA) and cobalt-based IMAC resin (Co-NTA), the latter is well known as Talon. This research studied the influence of metal ions Ni2+ and Co2+ in rhEPO purification. The results indicated that Ni- NTA had higher affinity in binding rhEPO protein compared with Co-Talon. Average total protein by Ni-NTA was 226.72 μg/mL and Co-Talon was 60.52 μg/mL, but the protein purity with Co-Talon was better than Ni-NTA. Base on protein yield that can be separated, Ni-NTA is more suitable to obtain high yield of rhEPO protein.
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