Judul: Over-expression of OsDREB1A gene for improvement of drought tolerance on rice.
Abstrak: Drought is a major factor that influence the growth and productivity of rice. Drought tolerant rice varieties are needed to increase rice production. Development of improved rice varieties can be done through classical breeding and genetic transformation. The research objective was to obtain rice plants containing the construct 35S::OsDREB1A. Agrobacterium tumefaciens mediated transformation of rice cv. Nipponbare using OsDREB1A gene under the control of CAMV 35 promoter produced 81 putative transgenic rice plants (T0) positively containing the transgene based on PCR analysis. The plant height, panicle number, panicle length, and the grain number of transgenic plants were in general lower than the Nipponbare wild type. T1 seeds from selected genotypes were screened for hygromycine resistence, and rice seedlings were evaluated for tolerance to drought stress in the glasshouse, based on the standard evaluation system for rice (IRRI 1996). Five independent transgenic rice plants were identified to be tolerant to drought stress. Molecular characterization using Southern hybridization showed that these plants had 2- 4 copies of transgene. Physiological analysis showed that the drought avoidance is the possible mechanism of adaptation to drought of the transgenic plants. The drought tolerant transgenic plants were crossed to Indonesian elite rice cultivars (Ciherang, Batutegi, Code, and Konawe). Glasshouse evaluation showed that F1 transgenic plants derived from cultivars Batutegi, Code, and Konawe were more tolerant to drought than their wild-type counterparts. F1 transgenic plants were backcrossed into corresponding elit rice cultivars, and PCR analysis showed that those plants contained the transgene. These results showed that 35S::OsDREB1A could be transferred and inherited to elite rice genotipes through crossing. The new drought tolerant germplasms generated in this research can be useful materials for breeding program for improvement of drought tolerance on rice in Indonesia.
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Judul: Karakterisasi Molekuler Tanaman Padi Nipponbare Transgenik 35S::OsERA1 yang Toleran Kekeringan
Abstrak: Drought is one of the factors that resulted in the growth and development of rice plants inhibited. One way to overcome this problem is use of transgenic rice plants that are tolerant to drought. The success of the transformation using OsERA1 gene in Nipponbare genotype rice requires further confirmation of tolerance to drought stress. The aim of this study was to obtain transgenic Nipponbare genotype rice contain gene 35S::OsERA1 drought tolerant, know the number of gene copies, and compare the contents and empty seeds with control. The evaluation of drought the tolerance was performed using Standard System Evaluation for Rice method (IRRI 1996). All tested transgenic genotypes were tolerant to drought. Molecular characterization using southern hybridization showed that transgenic rice plants had 1-3 copies of transgen. Drought stress inhibited plant growth, which lowers the amount of seed and increasing the void content. 18.5.4 and 6.4.10 genotypes are considered tolerant to drouht. 18.5.4 genotype have the number of grains per panicle contents of 36 seeds and 6.4.10 genotype have a 38.03% percentage of empty seeds
Keyword: transgenic plant, drought, southern hybridization, OsERA1 gene

Judul: Cycle Hamilton pada Graf Lengkap, Graf Regular, dan Graf 2-Connected 4-Regular Berorder Kurang dari Sepuluh
Abstrak: Suatu graf disebut graf Hamilton apabila graf tersebut memuat cycle Hamilton, yaitu cycle yang melewati setiap vertex tepat satu kali. Menentukan keberadaan cycle Hamilton pada suatu graf dapat menggunakan syarat cukup yang berupa teorema-teorema yang telah diperkenalkan sejak tahun 1950-an. Beberapa syarat cukup yang dibahas dalam karya ilmiah ini adalah syarat cukup yang berhubungan dengan graf lengkap dan graf regular. Suatu graf lengkap berorder 𝑛������ ≥ 3 dan graf r-regular berorder 𝑛������ ≤ 2𝑟������ + 1 merupakan graf Hamilton, Karena kedua graf tersebut memuat cycle Hamilton. Selanjutnya, syarat cukup tersebut digunakan untuk menentukan keberadaan cycle Hamilton pada graf 2-connected 4- regular berorder kurang dari 10.
Keyword: graf Hamilton, graf regular, graf lengkap, graf 2-connected 4-regular