Judul: Siklus Hidup Ichtophirius Multifiliis Fouquet
Abstrak: Suatu penelitian yang dilakukan di laboratorium Fakultas Perikanan Institut Pertanian Bogor dari tanggal 5 Oktober sampai dengan tanggal 11 Desember 1978 bertujuan untuk mengetahui siklus hidup (Ichthyophthirius multifillis Fouquet.
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Judul: Pemijahan, Embriologi dan Perkembangan Larva Ikan Menfis (Pterophyllum scalare)
Abstrak: Penelitian ini dilakukan mulai bulan Februari sampai Juli 1991 di Laboratorium Pengembangbiakan Ikan Fakultas Perikanan IPB, Darmaga. Penelitian ini bertujuan untuk mengamati tingkah laku induk ikan yang mijah, selang waktu pemijahan, fekunditas, telur yang diovulasikan, perkembangan embrio, perkembangan larva dan pertumbuhan juvenil ikan menfis. Induk ikan menfis yang digunakan sebanyak 5 pasang, strain marmer dan sleyer. Warna ikan adalah hitam, putih keperakan dan hijau zaitun. Telur yang digunakan untuk pengamatan perkembangan embrio masih hidup dan utuh. Larutan Methylene Blue, Novobiocin dan garam dapur digunakan untuk pencegahan terhadap penyakit. Makanan yang diberikan ke induk dan larva adalah cacing rambut dan nauplii Artemia sp.
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Judul: Molecular Identification of Mutant Candidate of Gα Gene from Soybean cv. Slamet Based on mRNA
Abstrak: Heterotrimeric G proteins composed of a, ß, and . subunits, are bound to the inside surface of the cell membrane. Ga proteins will be activated by extracellular stimuli (biotic or abiotic stress). It is presumably Ga proteins playing an important role in aluminum (Al)-stress tolerance. Soybean cv. Slamet was considered as a tolerant plant to Al and has a single copy of Ga gene. Gamma irradiation to soybean cv. Slamet seeds caused a random chromosomal mutation of second generation plants (designated as M2). The Ga mutant candidates were selected based on stomatal closure in the daylight and dwarf. Ga mutant candidates M4, number 338 and 103 have severe root cell tissues damage compared to non mutant ones. Plant number 338/3S1.174/12/14 (M4) and 103/3S3.103/8/5/1/4 (M5) have a Ga gene mutation at the U3-L4 sequences (exon 5 – exon 11). Whereas, plant number 344/3S1.196/14/1/8 (M4) is assumed as Ga gene mutant. All of those plants were replanted until sixth generation. The candidates of Ga mutant selected have more than fifty percent of closed stomata and dwarf. Four number of M5 plants and three number of M6 plants were selected for Ga mutant identification based on mRNA analysis. The fifth generation plant number 344/3S1.196/14/1/8/7 were decreased its Ga gene expression at L5-L4 sequences (exon 7 – exon 11). The M6 generation plant number 344/3S1.196/14/1/8/7/5 have mutation in Ga gene at L1-L3 sequences (exon 2 – exon 6). A major contributing factor to decreased expression levels of Ga gene may be due to mutation of Ga gene promotor. Three other of M5 plants were assumed as Ga mutant. M6 plant number 103/3S3.103/8/5/1/4/8 has mutation at L3-L2 sequences (exon 6 – exon 15) and number 103/3S3.103/8/5/1/4/9 has mutation at U3-L4 sequences (exon 5 – exon 11). The decreasing of gene expression caused by mutation in those plants was indicated by the less pronounced DNA bands on agarose gel than that of non mutant plants.
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