Judul: Yellows Disease on Carrots (Daucus carota L.) Associated with Phytoplasma in West Java.
Abstrak: Carrot (Daucus carota L.) is in the family Umbelliferae grown for its edible root. Carrots are widely cultivated in the highlands with loose soil type that rich of humus. It is an important vegetable plant in Indonesia and is widely consumed because it is rich in beta-carotene (A vitamin). Carrot cultivation in Indonesia was initially concentrated in West Java, such as in Cipanas (Bogor) and Lembang (Bandung), but currently it has spread to Central and East Java and outside Java due to its higher demand. One of the problems in carrot cultivation is pathogen infections that cause plant diseases and can potentially reduce yield quality and productivity. Diseases infecting carrot plants in Indonesia caused by viruses, bacteria, fungi and nematodes have been widely reported, but until now there have been no reported diseases caused by phytoplasma. Infection of phytoplasma has been reported to cause significant yield loss in some countries. Therefore, it is necessary to conduct research to determine the status of phytoplasma infecting carrot in Indonesia. Research was carried out with the following objectives: (1) Conducting observation and survey to confirm the presence of yellowing disease and leafhoppers in carrot growing areas in West Java; (2) Studying the morphological, histological and molecular properties of phytoplasmas associated with yellow disease in carrot in West Java; (3) Studying the potential of leafhoppers species Orosius argentatus and Balclutha incisa as the vector of yellow disease in carrot. Research activity began with a field survey on carrot plantations, followed by identification and characterization of phytoplasma in the laboratory and transmission assay of phytoplasma through leafhoppers in a screenhouse. During the survey in Bogor, Cianjur and Bandung, yellowing symptoms and the presence of leafhoppers were observed. Plant samples from the field were brought to the laboratory for identification of phytoplasma. The identification method consisted of microscopic observation using a transmission electron microscope (TEM), molecular detection using nested-polymerase chain reaction (nested-PCR), followed by cloning of phytoplasma DNA. Planthopper samples from the field were brought to the laboratory for identification based on morphological characters and the molecular identification of phytoplasmas associated with planthoppers. Furthermore, sequence analysis was carried out based on the 16S rRNA gene using the BLASTn, phylogenetic analysis, and in silico RFLP. The incidence of yellow disease in carrots is low, ranging from 6.22 - 7.87% in Bogor, 7.47% in Cianjur, and 5.97% in Bandung. Five species of leafhopper were found in carrot fields showing yellow symptoms in Bogor and Cianjur, i.e. B. incisa, Cicadulina bipunctata, Empoascanara indica, Exitianus indicus, O. argentatus and one species of planthopper, Sogatella furcifera. Molecular detection using nested-PCR confirmed the association of phytoplasma with yellow symptom in carrot and 4 planthopper species. Observations using TEM showed that there were abnormalities in the shape and integrity of cells in carrot leaf tissue with yellow symptoms compared to healthy plant cells. In addition, phytoplasma cells were found in the phloem tissue of symptomatic plant leaves with a rounded (spherical) but not fixed (pleomorphic) shape measuring about 0.2-0.5 m. Chlorophyl content of symptomatic leaves decreases 0.106%, but carbohydrate content as starch and reducing sugar
Keyword: analisis filogenetik, gen 16Sr RNA, insidensi penyakit, nested-PCR, wereng daun

Judul: Identifikasi Spesies Polerovirus pada Tanaman Wortel melalui Analisis Sekuen Nukleotida Gen Coat Protein
Abstrak: During survey on central production of carrot in West Java, some plants were found exhibiting virus-like induced symptoms. The diseased plants showed reddening and yellowing of leaves, but the plants were unlikely to be stunting. The symptom was resemble to the disease reported in other countries as „motley dwarf‟, in which the reddening, yellowing, and stunting is accentuated. The disease was reported to be caused by mixed infection of two different viruses that were Carrot red leaf virus (CtRLV), member of genus Polerovirus and Carrot mottle mimic virus (CMoMV), member of genus Umbravirus. To know the causal agent of the disease present in Indonesia, total RNA extraced from the symptom exhibiting carrot plants obtained from field in Cipanas, Cianjur, West Java was subjected to reverse transcription-polymerase chain reaction (RT-PCR) using primer pair specific to CtRLV. Unfortunately, the virus was failed to be detected and therefore, further identification was focused on other virus. Using primer pair of CP-F (5‟-AATTAAGGATCCAATACGGGAGGGGTTAGGAGAAAT-3‟) and CP-R (5‟-AATTAACTGCAGTTTCGGGTTGTGCAATTGCACAGTA-3‟), that were specific to Polerovirus. RT-PCR was successfully amplified a DNA band of about 650 bp, the size which accordance with the designed primers. The RT-PCR product was then sequenced and the 630 nucleotides were succesfully read. The BLAST analysis of the nucleotide sequence revealed that the virus associated with red leaf disease on carrot in West Java was associated with Pepper vein yellow virus (PeVYV). PeVYV was a virus found previously infected chilli pepper in Bali. This is the first report concerning PeVYV infected carrot in Indonesia.
Keyword: reverse transcriptionpolymerase chain reaction, red leaf disease, Pepper vein yellow virus

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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