Judul: Performa Reproduksi Ikan Mas (Cyprinus carpio L) Jantan Transgenik Hormon Pertumbuhan Generasi Kedua
Abstrak: Fertility and viability are common parameters used to evaluate environmental safety as well as breeding potency of transgenic organisms. This research was performed to evaluate the reproductive performance of F2 generation GH (growth hormone) transgenic male common carp. This research used completely random design with two factors (transgenic and non-transgenic) and three replications. Three GH transgenic male common carp (TGJ) and three non-transgenic (NTJ) fish were crossbred with two non-transgenic female common carps (NTB), resulting 12 families. Parameters observed were semen volume, motility, duration of motility, number of sperm cells, survival of embryo, hatching rate, survival of larva until 3 days old, and morphology of sperm and larva. The results showed that semen volume of TGJ and survival of juvenile in 30 days was higher compared to NTJ (p<0,05), while motility rate, duration of motility, number of sperm cells, survival of embryo, hatching rate, and survival of larva were similar (p>0,05). Furthermore, there were no abnormalities found in sperm morphology of TGJ and the hatched larva of TGJ x NTB crossbred were the same with NT. As the conclusion, reproductive performance of GH transgenic male common carp showed good and similar result with the non-transgenic fish.
Keyword: reproductive performance, male broodstock, tiGH gene, common carp

Judul: Introduksi dan Ekspresi Gen Hormon Pertumbuhan pada Ikan Mas Transgenik
Abstrak: Common carp Cyprinus carpio is one of the economically valuable aquaculture fish species in Indonesia. This research was aimed to produce transgenic common carp that grow rapidly by over-expressing the tilapia growth hormone (tiGH) gene. Trangenic common carp were produced by sperm-mediated gene transfer using electroporation method. In this study, two electroporation programs that developed by other researcher were applied to obtain a suitable electroporation method for common carp. The electroporated sperm were then used to fertilize eggs to produce F0 transgenic fish. Transgenic F0 carrying tiGH in their sperm were crossed with non-transgenic fish to produce transgenic first (F1) generation. Identification of transgenic fish was performed by PCR method using specific primer for tiGH gene. Expression of tiGH was analyzed using RT-PCR method and growth measurement at F1 fish generation. The results showed that electroporation of sperm using 50 Volt/cm, and DNA concentration of 25 ng/μl was produced higher percentarge of fish carrying tiGH in their gamet compared to that of another electroporation program (40 Volt/cm, DNA concentration of 50 ng/μl). Five sexually matured males F0 transgenic fish were selected and mated with non-transgenic to produce F1 generation. Percentage of F1 progenies carrying tiGH was 47,2-66,7%. Variated measure of F1 genereations was 10,1->12,0 cm size (22,5%-42,9%), 8,1->10,0 cm size (14,3%-42,9%), 6,1->8,0 cm size (11,9%-29,2%), 4,1->6,0 cm size (0,0%-39,3%). Increasing of growth of F1 was about 1,4-1,87 times for non transgenic fish. Survival of transgenic and non-transgenic F1 was generally similar. RT-PCR analysis showed that the F1 transgenic fish have tiGH gene expression in caudal fin, while no expression in non-transgenic fish were found. Thus, the increased body weight of F1 transgenic common carp was associated with expression of tiGH gene, and this fish can be used as broodstock for the production of fast-grow stable transgenic common carp.
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Judul: Simulasi Vaiabel Iklim pada Erupsi Gunung Api dengan Model WRF-Chem (Studi Kasus: Erupsi Gunung Kelud 13 Februari 2014)
Abstrak: Indonesia sering mengalami gempa tektonik maupun vulkanik karena dilalui oleh “Cincin Api Pasifik”. Letusan gunung api merupakan salah satu bencana alam yang perlu diwaspadai. Langkah preventif telah dilakukan oleh Pusat Vulkanologi dan Mitigasi Bencana Geologi (PVMBG) seperti penempatan alat observasi di 69 dari 127 gunung api aktif di Indonesia untuk mengukur seismik. Salah satu gunung api aktif di Indonesia adalah Gunung Kelud. Gunung Kelud terakhir meletus pada tanggal 13 Februari 2014 pukul 22.50 WIB. Penelitian mengenai deteksi dan prediksi aktivitas gunung api sering dilakukan oleh peneliti vulkanologi dengan mengamati parameter geofisika: seismik, deformasi tanah, perubahan gravitasi, suhu air kawah, warna air kawah, dan tinggi muka air tanah. Akan tetapi, penelitian tentang gunung api terkait dengan deteksi aktivitas vulkanik masih jarang dan umumnya terkait dengan perubahan iklim. Peneliti mengembangkan dan menggunakan model-model iklim dan satelit untuk mempelajari dampak erupsi gunung api terhadap iklim dan mendeteksi aktivitasnya. Model iklim, seperti Weather Research Forecasting (WRF) berpotensi digunakan untuk mendeteksi erupsi gunung api melalui pendekatan deteksi perubahan titik (change-point detection). Penelitian ini bertujuan mengevaluasi dan mendeteksi perubahan data deret waktu dari luaran WRF-Chem pada kejadian erupsi Gunung Kelud 13 Februari 2014 dengan variabel iklim yang dianalisis, yaitu suhu permukaan tanah, suhu udara, dan kelembapan relatif. Metode yang dipilih untuk mendeteksi perubahan data deret waktu adalah Noise-Based Change Point (NBCP), sedangkan untuk evaluasi model adalah koefisien korelasi Pearson (r) dan root mean square error (RMSE). Model WRF-Chem membutuhkan data masukan berupa data cuaca dan kimia vulkanik, yaitu Global Forecast System (GFS) dan Eruption Source Parameters (ESP). Data pendukung seperti produk MODIS yang dianggap sebagai data observasi diperlukan untuk menilai kemampuan dari WRF-Chem untuk waktu analisis mulai 1 Desember 2013 00.00 UTC hingga 22 Maret 2014 23.00 UTC. Penggunaan parameterisasi di dalam simulasi WRF-Chem dipilih berdasarkan penelitian sebelumnya dan dievaluasi dengan data lapangan yang dilakukan pengukuran pada 31 Juli 07.00 WIB – 1 Agustus 2019 06.55 WIB. Hasil simulasi WRF-Chem pada 31 Juli 07.00 WIB – 1 Agustus 06.55 WIB menunjukkan suhu permukaan tanah, suhu udara, dan kelembapan relatif sesuai dengan pengukuran lapangan (r = 0,94; RMSE = 1,97oC, r = 0,97; RMSE = 0,63oC, r = 0,89; RMSE = 9,44%). Ketika dievaluasi menggunakan data MODIS, hanya suhu udara yang sesuai (r = 0,99; RMSE = 2,81oC), sedangkan belum sesuai pada suhu permukaan tanah dan kelembapan relatif. Hasil perhitungan dengan metode NBCP dan smoothing bahwa terdeteksi perubahan meningkat pada suhu permukaan tanah pada 22 Januari 2014 00.00 UTC atau 22 hari 15 jam sebelum erupsi., Indonesia frequently experiences tectonic and volcanic earthquakes because it is traversed by the "Ring of Fire". Volcanic eruption is a natural disaster that needs to be alert. Preventive approach was taken by the Center for Volcanology and Geological Hazard Mitigation (CVGHM) such as installing the observation instruments at 69 of 127 Indonesia volcanoes to measure seismic. One of active volcanoes in Indonesia is Mount Kelud. Mount Kelud last erupted on February 13, 2014 22.50 WIB. Research on detection and prediction of volcanic activity is often carried out by volcanology researchers by observing geophysical parameters: seismic, soil deformation, gravity change, crater water temperature, crater
Keyword: air temperature, land surface temperature, noise-based change point, relative humidity