Judul: Strategi Pengelolaan Kawasan Wisata berdasarkan Willingness to Pay dan Daya Dukung Kawasan (Studi kasus: Pantai Sulamadaha, Kelurahan Sulamadaha, Kecamatan Ternate Barat, Kota Ternate, Provinsi Maluku Utara)
Abstrak: Pantai Sulamadaha merupakan salah satu kawasan wisata bahari yang terletak di Provinsi Maluku Utara. Kencenderungan peningkatan jumlah kunjungan tahun 2011 hingga 2015 berpotensi menimbulkan overcarrying capacity (OCC). Penelitian ini bertujuan untuk mengestimasi daya dukung kawasan dari atraksi wisata Pantai Sulamadaha, mengestimasi tarif masuk wisata berdasarkan WTP (Willingness to Pay) pengunjung untuk mengontrol jumlah pengunjung sesuai carrying capacity di Pantai Sulamadaha, serta menganalisis strategi pengelolaan kawasan wisata berbasis daya dukung di Pantai Sulamadaha. Hasil penelitian menunjukkan spot hol sulamadaha mengalami OCC pada aktivitas snorkeling dan rekreasi pantai baik pada peak season maupun tanpa mempertimbangkan peak season sedangkan spot pantai pasir hitam cenderung under carrying capacity. Estimasi tarif berdasarkan WTP memperoleh hasil sebesar Rp5,700. Penetapan tarif tersebut akan meningkatkan pendapatan sebesar Rp219,735,600 atau dengan rasio sebesar 112.04%, namun belum sepenuhnya menghilangkan fenomena OCC. Analisis SWOT menempatkan Pantai Sulamadaha pada sel II yang mengindikasikan berada pada posisi tumbuh dan membangun. Adapun strategi yang dapat dilakukan diantaranya menetapkan segmentasi tarif berdasarkan spot wisata yang dikunjungi serta menggalakkan promosi wisata baik melalui media cetak, elektronik, ataupun sosial media.
Keyword: Overcarrying Capacity, SWOT, Tarif, WTP

Judul: Beach Tourism Development Strategy in Ternate City, North Maluku Province
Abstrak: Kota Ternate Provinsi Maluku Utara memiliki potensi yang besar dalam sektor pariwisata, letak geografis Kota Ternate yang strategis menyebabkan tingkat kunjungan wisatawan yang tinggi baik wisatawan lokal maupun mancanegara, memiliki keanekaragaman obyek dan daya tarik wisata, memiliki karakteristik kota sebagai kota pantai (waterfront city) dan peluang peningkatan pendapatan anggaran daerah (PAD) dari sektor pariwisata. Ditinjau dari kebijakan yang ada saat ini, baik dari tingkat pusat maupun daerah mendukung untuk pengembangan pariwisata di Kota Ternate beberapa hal yang diamanatkan seperti penyediaan sarana dan prasarana pariwisata, pembangunan aksesibilitas pariwisata, serta pembangunan daya tarik wisata. Namun kondisi saat ini potensi pariwisata di sepanjang pantai Kota Ternate belum tergali secara maksimal. Tujuan penelitian ini yaitu: (1) menganalisis obyek wisata pantai yang sudah berkembang, yang berpotensi untuk dikembangkan dan yang kurang berpotensi untuk dikembangkan, (2) menganalisis faktor-faktor penarik minat wisatawan pada obyek wisata pantai di Kota Ternate, (3) menyusun strategi pengembangan pariwisata pantai di Kota Ternate Metode analisis yang digunakan meliputi analisis Multi-Criteria Evaluation (MCE) untuk mengetahui obyek wisata yang sudah berkembang, yang berpotensi dikembangkan dan kurang berpotensi dikembangkan, analisis regresi logistik biner untuk mengetahui faktor-faktor penarik minat berkunjung wisatawan ke obyek wisata pantai di Kota Ternate, metode hybrid Analytic Hierarchy Process (AHP) dan SWOT (Strenghts, Weakness, Opportunities, and Thereats) (A’WOT) untuk menyusun strategi pengembangan pariwisata pantai di Kota Ternate. Hasil penelitian didapatkan bahwa dari 9 obyek wisata pantai, 5 diantarannya masuk ke dalam klasifikasi obyek wisata sudah berkembang yakni Pantai Sulamadaha, Pantai Jikomalamo, Pantai Tobololo, Pantai Bubane Ici, dan Pantai Kastela. Dua obyek wisata pantai yang berpotensi untuk dikembangkan yakni Pantai Tabanga, dan Pantai Hol. Sementara itu, 2 obyek wisata yang kurang berpotensi untuk dikembangkan adalah Pantai Ake Rica dan Pantai Talaga Nita. Faktor-faktor signifikan yang mempengaruhi terhadap minat berkunjung wisatawan pada obyek wisata pantai di Kota Ternate yaitu :: umur, jenis kelamin, frekuensi berkunjung, biaya berkunjung dan fasilitas. Strategi prioritas pengembangan pariwisata pantai di Kota Ternate terdiri dari: pengembangan obyek wisata dengan memanfaatkan keunikan, pegadaan dan peningkatan program community based tourism, meningkatkan penyelenggaraan event-event kepariwisataan, peningkatan sarana akomodasi, pengembangan sektor pariwisata sebagai sektor unggulan, dan mempertegas perizinan bahan galian C di beberapa kawasan obyek wisata pantai, The City of Ternate, North Maluku Province, has great potential in tourism sector, the strategic geographical location of Ternate City causes it to have a high level of tourist visits, both local and foreign tourists, has a variety of tourist objects and attractions, has the characteristics of the city as a coastal city (waterfront city) and opportunities to increase regional budget revenue (PAD) from the tourism sector. According to the current policies, both at the central and regional levels to support the development of tourism in the City of Ternate, there are several things that are mandated, such as the provision of tourism facilities and infrastructure, development of tourism accessibility, and development of tourist attractions. However, the current condition of the tourism potential along the coast of Ternate City has not been optimally explored. The aims of this study are: (1) to analyze beach tourism objects that have been developed, those that have the potential to be developed and
Keyword: A’WOT, Beach town, Multi criteria evaluation, Binary logistic  	regression, Development strategy

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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