Judul: Aspek manajemen dan gizi makanan yang disediakan taman kanak-kanak di kota Bogor
Abstrak: Secara umum tujuan penelitian ini adalah mengkaji aspek manajemen dan gizi makanan yang disediakan Taman Kanak-Kanak di Kota Bogor. Tujuan khusus penelitian ini adalah (1)menguraikan manajemen penyelenggaraan makan di TK, (2) menguraikan penerapan prinsip-prinsip sanitasi dan higiene makanan yang disediakan TK, (3) menguraikan kualitas menu (jenis dan variasi) makanan yang disediakan TK, (4) menghitung kandungan gizi makanan yang disediakan TK, dan (5) menguji perbedaan tingkat pengetahuan gizi kesehatan guru pada TK yang menyelenggarakan makan bersama dengan frekuensi yang berbeda.
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Judul: Materi pengajaran dan tingkat pengetahuan gizi kesehatan guru taman kanak-kanak di Kota Bogor
Abstrak: Secara umum tujuan penelitian ini adalah menguraikan materi pengajaran dan tingkat pengetahuan gizi kesehatan guru Tarman Kanak-kanak di Kota Bogor. Tujuan khusus penelitian ini adalah (I) menguraikan tema pelajaran yang digunakan untuk menyampaikan materi gizi kesehatan, (2) menguraikan materi pengajaran dan kegiatan gizi kesehatan di TK, (3) menguraikan pemahaman guru TK mengenai materi gizi kesehatan yang masih perlu ditambahkan (4) menguraikan metode pengajaran pendidikan gizi kesehatan yang dilakukan oleh guru TK, (5) menguraikan tingkat pengetahuan gizi kesehatan guru TK, dan (6) menguji hubungan antara usia, tingkat pendidikan, kualifikasi pendidikan dan lama kerja dengan tingkat pengetahuan gizi kesehatan guru TK.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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