Judul: Interaksi Masyarai{at dengan Taman Nasional Alas Purwo (Studi Kasus di Desa Kendalrejo Kecamatan Tegaldlimo Kabupaten Banyuwangi Propinsi Jawa Timur)
Abstrak: 8erubahnya status suatu kawasan menjadi taman nasional tclah mengakibatkan masyarakat sekitar yang sudah sejak dulu berinteraksi dengall kawasan taman nasional secara de jure kehilangan akses terhadap sumberdaya di dalam kawasan tersebut. Bahkall mcreka seringkali dianggap sebagai ancaman terhadap upaya konservasi dan peiestarian taman nasional. Inilah yang kemudian menyebabkan hubungan antara pengelola dengan masyarakat sekitar menjadi kurang hannonis, dan pada akhirnya dapat memicu konflik kepentingan di antara keduanya.
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Judul: Interaksi Masyarakat Suku Asli (Masyarakat Adat) dengan Masyarakat Pendatang dan Implikasinya pada Rancangan Pengelolaan Taman Nasional Wasur
Abstrak: Wasur National Park (WNP) was one of Indonesia Natural Reserve Areainhabited by 68,8% native communities namely Marind Imbuti, Kanume, Marori Men Gey and Yeinan, also by 31,18% immigrants originating from Java, Sulawesi and Maluku.This research was aimed to formulate the implication of interaction between native communities with the immigrants for the management of WNP. The natives wisdoms were in the form of sasi tradition, wisdoms toward the sacred places, totemism, hunting and education system. Socio-economic interactions among the natives were expressed on the forms oflanduse and water consumption which might betrigger for conflicts, as happenened at the Tomer village between theKanume and the immigrants from Sulawesi. This interaction also established within the field of agriculture, labour, financial capital and land cultivation. Socio-cultural interaction were expressed through marriage, beliefs, religions, art and language, whilesocio-ecology interaction were shown by the similarity of resource exploitation pattern, tradition symbols and sacred places. Immigrants from Maluku also managed to establish an interaction in a matter of utilization regulation which influenced by the church sasi. The implications of the interaction of native communities with the immigrants for the management of WNP is the creation of regional regulations, zoning, law enforcement, cultivation of plants and animals ofnative communities’ totems, alternative natural resource utilization, community training and assistance which consider the presence and interest of the community itself.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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