Judul: Produksi dan Uji Bioaktivitas Protein Rekombinan Hormon Pertumbuhan Ikan Mas.
Abstrak: Teknologi rekayasa DNA dan protein telah merambah dunia perikanan. Rekayasa DNA seperti transgenik bermanfaat untuk menyisipkan gen-gen yang berguna dan menghasilkan ikan budidaya dengan karakter unggul. Contoh penggunaan teknologi transgenik adalah penyisipan gen penyandi hormon pertumbuhan (growth hormone (GH)) yang dapat memacu pertumbuhan ikan menjadi beberapa kali lipat dari ikan normal (Lutz 2001), anti freeze protein (AFP) yang dapat meningkatkan toleransi ikan pada suhu dingin (Beardmore & Porter 2003) dan pigmen warna yang dapat menghasilkan ikan hias yang lebih menarik dengan beraneka ragam warna. Sementara itu, rekayasa protein yang bermanfaat bagi ikan secara massal dilakukan dengan menggunakan bakteri, ragi, maupun sel sebagai media perbanyakannya (Sekine et al. 1985; Mahmoud 1999; Anathy et al. 2001; Li et al. 2003).
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Judul: Pertumbuhan benih ikan betok (Anabas testudineus, Bloch) yang disuntik hormon pertumbuhan rekombinan ikan kerapu kertang dengan dosis berbeda
Abstrak: Penelitian dilakukan untuk menentukan dosis hormon pertumbuhan rekombinan (recombinant growth hormone, rGH) yang sesuai pertumbuhan ikan betok Anabas testudineus. Penelitian ini dilakukan dengan tiga perlakuan dosis rGH (0 μg rGH/g bobot tubuh, 0,5 μg rGH/g bobot tubuh dan 1,5 μg rGH/g bobot tubuh) dan satu kontrol. Setiap perlakuan diulang sebanyak tiga kali. Produksi protein rGH dilakukan menggunakan bakteri Escherichia coli BL21 sehingga dihasilkan pelet rGH yang digunakan untuk penyuntikan pada ikan. Hasil penelitian menunjukkan bahwa pemberian rGH melalui injeksi dapat meningkatkan pertumbuhan dan kelangsungan hidup ikan betok. Dosis rGH yang baik untuk meningkatkan pertumbuhan dan mempertahankan kelangsung an hidup ikan betok melalui injeksi adalah 0,5 μg rGH/g bobot tubuh ikan betok.
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Judul: Thermal Characteristics and Dynamics of Tropical Cyclone over Western North Pacific Ocean: A Case Study of Typhoon Songda (Chedeng) 2011
Abstrak: The frequent occasion of Tropical Cyclone (TC) over Western North Pacific (WNP) Ocean lies from 10˚N to 26˚N and 121˚E to 170˚E. Sources of TC development come from warm ocean temperature where latent heat is released during condensation processes and subsequently concentrated in the boundary layer. Relative humidity at 850-700 hPa is responsible to produce the most intense storm. Meanwhile, weak vertical wind shear between 850-200 hPa is one of the dynamical factors that lead to the development of TC. Pressure level of 850-200 hPa is chosen to minimize the thermodynamic effect in the lower troposphere. Typhoon Songda (Chedeng in the Philippines) or TSC lasted from May 18th to 30th, 2011 and strengthened into powerful Super Typhoon stage between May 24th and 27th. Long curving track and the exhibition of all sequences of TC life cycle, TSC is then considered as an ideal case of TC. The objective of the study with case of TSC is to investigate the role of thermal characteristics and dynamics on TC intensity. The research is carried out by using Advanced Research of Weather Research and Forecasting model (WRF ARW model) version 3.3. Two domains are employed in the model. The first domain situate from 02˚N to 40˚N and 118˚E to 149˚E and cover spatial resolution () of 33.1 km. The second domain situate from 09˚N to 27˚N and 119˚E to 132˚E with of 11 km. Timestep of the model () is 120 seconds. The model is supported by the following physical parameterization, i.e., Yonsei University Scheme (YSU) in the boundary layer, Kain-Fritsch scheme (KF) in the cumulus, and WRF Single-Moment 3-class scheme in the microphysics. Initial meteorological condition prior to the formation of the cyclone is generated using WRF ARW standard-release model. Sensitivity of the model output is obtained from a selected single-parameter change scenarios, namely, 10 % to 20 % decrease and increase of wind shear initial data at 850-200 hPa, and by 10 % to 20 % decrease of relative humidity initial data at 850-700 hPa. The result showed that the negative correlations between wind shear and intensity are found in wind shear scenarios. Wind shears of 10 ms-1 which lead to strengthening of the wind intensity in the early period of Typhoon development are found in wind shear scenarios. The effect of wind shear changes on wind intensity is statistically significant in the 20 % wind shear scenarios. During mature stage, weak wind intensity occurred when wind shear increase as given in the WRF standard-release and the 20 % decreasing scenario. Different situation is found in the 20 % increasing scenario where wind shear tend to weak and lead to strong wind intensity at this stage. Meanwhile, the reduction of moisture supply at 850-700 hPa resulting from 20 % decreasing relative humidity play a major role in the weakening of wind intensity and reduces mechanical energy of the cycle process as much as 300 J kg-1.
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