Judul: Prediksi Potensi Cadangan Airtanah Menggunakan Persamaan Darcy di Kota Tangerang Selatan, Provinsi Banten
Abstrak: Water is very important for human beings. Sources of freshwater that can be used is only 3% of the total water availability on earth. One of fresh water’s sources that can be used is groundwater. The objective of this study was to determine the capacity of groundwater storages (discharge) in aquifers at South Tangerang city using the Darcy equation. The method of research was include several stages, such as the preparation phase, data collection, data processing, data analysis, and presentation of results. Data were obtained from geoelectric measurements using Schlumberger software, and were used to analyse the type and depth of rock layers. Beside, the data were also used to determined the position of confined and unconfined aquifer. The flownet of confined and unconfined aquifer indicated the scheme of groundwater’s flow and was used to decide the cross section of the flow. Darcy equation required another parameter called hydraulic conductivity. In this research, the aquifer was dominated by sand, so the hydraulic conductivity for sand (12 m/day) was used in calculation. As the result groundwater storages on unconfined aquifer was 3551,50 m3/day, while on confined aquifer was 16864.11 m3/day.
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Judul: Prediksi Potensi Cadangan Airtanah Menggunakan Persamaan Darcy Di Kabupaten Tangerang, Provinsi Banten
Abstrak: Water is very important for human beings. Only 3% of the total water availability on earth is supplied by water resourses. Other alternative is groundwater or water inside aquifer. The objective of this study was to predict the capacity of groundwater in aquifers in Tangerang regency using the Darcy equation. Theresearch consisted of several stages, such as the preparation phase, data collection, data processing, data analysis, and presentation of results. Data were obtained from geoelectric measurements using Schlumberger software, and were used to analyse the type and depth of rock layers. Beside, the data were also used to determined the position of confined and unconfined aquifer. The flownet of aquifer indicated the scheme of groundwater’s flow. Darcy equation required another parameter called hydraulic conductivity. The average of Hydraulic conductivity 1 m/day for confine aquifer and 6,1 m/day for unconfine aquifer was used in calculation. As theresult prediction of groundwater storages on unconfined aquifer was 776,47 m3/day, while on confined aquifer was 4342,15 m3/ day
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Judul: Pemurnian dan pencirian protease serina dari ekstrak jamur shiitake (Lentinus edodes)
Abstrak: Shiitake (Lentimus edodes) adalah jamur untuk kesehatan yang rendah kalori, tinggi kandungan protein, kitin, zat besi, zink, serat, asam amino esensial, vitamin, dan mineral. Jamur shiitake telah digunakan lebih dari dua ribu tahun sebagai makanan dan obat tradisional di Jepang. Penelitian ini bertujuan memurnikan dan mencirikan protease serina dari ekstrak jamur shiitake. Ekstrak protease kasar diperoleh dengan cara ekstraksi jamur shiitake dalam 50 mM bufer universal pH 7. Ekstrak enzim dimurnikan dengan presipitasi amonium sulfat kejenuhan 70%, dialisis (cut off 10 kD), dan kromatografi penukar ion DEAE Sepharose. Pemurnian menghasilkan dua fraksi enzim aktif. Aktivitas spesifik tiap fraksi adalah 6,855 U/mg (fraksi 7) dan 10,098 U/mg (fraksi 19). Eluat DEAE memiliki tingkat kemurnian 1,89 dan 2,75 kali lebih tinggi terhadap ekstrak kasar dengan tingkat perolehan menurun hingga 0,4%. Hasil analisis SDS-PAGE 12% memperlihatkan fraksi protease terdiri atas 2 pita protein pada fraksi 7 (5) dan 47 kD) dan empat pita pada fraksi 19 (65, 51, 45, dan 37 kD). Aktivitas optimum protease dicapai pada subu 50°C dan pH 7. Uji zimogram 10% memperlihatkan bahwa protease mampu menghidrolisis substrat kasein, fibrinogen, albumin, dan gelatin dengan konsentrasi 1% (b/v). Aktivitas enzim protease kasar meningkat dengan adanya penambahan ion Na dan Fe (5 mM). Protease ekstrak kasar dihambat spesifik oleh inhibitor fenilmetilsulfonilklorida, N-p-tosil-L-lisin klorometilketon, dan soybean trypsine inhibitor sehingga digolongkan ke dalam kelompok protease serina serupa tripsin.
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