Judul: Identifikasi Keragaman Gen Hormon Pertumbuhan (GH|MspI) Pada Sapi Friesian Holstein Di Bib Lembang, BBIB Singosari, Dan BET Cipelang
Abstrak: Growth hormone (GH) is an anabolic hormone synthesized and secreted by somatotroph cells from the anterior lobe of the pituitary. The GH plays an important role in postnatal growth and development, tissue growth, lactation, reproduction, and metabolism. This study was aimed to identify polymorphism of the growth hormone gene (GH) in dairy cattle and beef cattle as a comparison. Holstein Friesian (HF) cattle for a total number of 89 heads from BIB Lembang (17), BBIB Singosari (32) and BET Cipelang (40); and four breeds of beef cattle for a total number of 37 heads from BET Cipelang were genotyped at intron 3 of the GH gene using PCR-RFLP method by MspI restriction enzyme. Genotyping the GH gene resulted in three genotypes, namely GH|MspI (+/+), (+/-), and (-/-), with two alleles, namely GH|MspI (+) and (-). Genetic polymorphism was detected in HF cattle and beef cattle, the exception was for Simental and Angus. The frequency of the GH|MspI (+) allele contrast to the GH|MspI (-) allele for HF cattle were 0.826 vs 0.174. GH|MspI (+/+) genotype had the highest frequency for HF (0,826), Simental, and Angus (1,000); but very limited for Limousin and Brahman (0,144 and 0,000). Chi-Square analysis showed that HF from the three locations were in Hardy-Weinberg equilibrium (χ2 < χ2 (0,05)), but contrast on beef cattle (χ2 > χ2 (0,05)). The value of heterozigosity expectation (He) for HF and beef cattles were estimated between 0.000-0.800. The genetic variation of the GH|MspI gene was low in most of these cattles, the exception was for Brahman.
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Judul: Identifikasi Keragaman Gen Growth Hormone Releasing Hormone (GHRH|HaeIII) Pada Sapi Friesian Holstein Di BIB Lembang, BBIB Singosari, Dan BETCipelang
Abstrak: This study was aimed to identify polymorphism of the GHRH gene in Holstein Friesian (FH) cattle from BIB Lembang, BBIB Singosari, and BET Cipelang; and beef cattle from BET Cipelang for a comparison. Blood samples used were 126 samples, consisting of 49 HF bulls from BIB Lembang (17 hds), BBIB Singosari (32 hds), and HF cows from BET Cipelang (40 hds); and a number of 37 hds of beef cattle consisting of Simmental (13 hds), Limousin (14 hds), Brahman (5 hds), and Angus (5 hds) from BET Cipelang. This study used PCR-RFLP method using restriction enzyme HaeIII. This enzyme restricted the GHRH gene sequences at the bases GG | CC at the positions of 118, 312, and 406 bp resulting four fragments with the lengths of 118, 194, 94, and 45 bp respectively. Genotyping GHRH gene produced two alleles, namely A and B alleles. These alleles produced three genotypes, namely the genotypes of AA (312, 94, and 45 bp), AB (312, 194, 118, 94, and 45 bp), and BB (194, 118, 94, and 45 bp). Frequency of the B allele was higher than the A allele. Frequencies of the A and B alleles in HF were in BIB Lembang 0,235 and 0,765, BBIB Singosari 0,234 and 0,766, and BET Cipelang 0,225 and 0,775. Chi-square analysis showed that all of HF and beef cattles were in Hardy-Weinberg equilibrium (χ2 < χ2 (0.05)), the exception in HF cattles in BIB Lembang, Brahman and Angus cattles in BET Cipelang. The highest heterozigosity values in HF was found in BIB Lembang (0,471), while the lowest was in BBIB Singosari (0,344). Heterozygosity values in the HF were lower than those of Simmental and Limousin beef cattle. GHRH gene in HF and beef cattle were polimorfic, the exception in Brahman cattle as possesing only the B allele.
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Judul: Alat Perekam Data Hujan dengan Mode Hemat Daya
Abstrak: Pengukuran intensitas hujan dapat dilakukan dengan beberapa pendekatan, seperti menggunakan perekam data hujan otomatis (automatic rain recorder). Data curah hujan yang terukur oleh tipping bucket akan tersimpan dalam SDcard setiap kejadian hujan terjadi. Penggunaan arduino sebagai komponen utama dipilih karena perangkat open source yang mudah dimodifikasi. Beberapa komponen arduino yang dapat dimodifikasi adalah ATmega dengan konsumsi daya sebesar 12 mA pada 3 volt serta 17 mA pada 5 volt. Adapun FTDI mengkonsumsi daya sebesar 20 mA pada 5 volt. Voltage regulator mengkonsumsi daya sebesar 38 mA pada 5 volt, 64 mA pada 9 volt, 103 mA pada 12 volt, selain itu led mengkonsumsi 1 mA pada 5 volt. Komponen yang dapat dihilangkan pada arduino antara lain FTDI, voltage regulator, serta led. Pengurangan komponen ini bertujuan agar daya tahan baterai menjadi 6.5 kali lebih hemat dibandingkan arduino standar. Setelah bagian bagian tersebut dihilangkan maka total konsumsi daya yang diperlukan oleh mikrokontroler on board arduino adalah 12 mA saat 3 volt dan 17 mA saat 5 volt saat mode kerjanya. Hasil pengujian menunjukkan bahwa pada penggunaan mode sleep lebih efisien daya dibandingkan mode power save. Mode sleep membantu cara penghematan daya pada pembuatan alat perekam data hujan Penggunaan metode ini jika diasumsikan kejadian hujan hanya terjadi selama tiga jam dalam sehari maka dengan menggunakan baterai sebesar 400 mAh dengan tegangan 3 volt maka alat perekam data hujan ini dapat hidup selama 15 Hari….
Keyword: Arduino, Power save, Rain, Automatic recorder, Tipping bucket, Geophysics and Meteorology, Rain fall, Model penakar hujan, Arduino promini, Arduino uno