Judul: Keragaman Genetik Ikan Kiper (Scatophagus argus) Berdasarkan  Marka Gen 16s rRNA di WPP 573 Bagian Barat sebagai Dasar Pengelolaan
Abstrak: Ikan kiper (Scatophagus argus) merupakan salah satu ikan demersal yang memiliki nilai ekonomis penting, ikan ini dijadikan sebagai ikan konsumsi dan ikan hias. Ikan kiper juga merupakan ikan yang aktif bermigrasi sehingga memungkinkan adanya perpindahan genetik dari perairan yang berbeda. Penelitian ini bertujuan untuk menganalisis keragaman genetik ikan kiper di WPP 573 Bagian Barat berdasarkan marka gen 16s rRNA sebagai pengelolaan sumberdaya yang berkelanjutan. Hasil amplifikasi menunjukkan amplicon dengan pita untaian tunggal namun masih terdapat smear akibat adanya kontaminan. Hasil sekuensing dan validasi BLASTn menunjukkan bawah seluruh contoh sampel ikan kiper termasuk ke dalam spesies Scatophagus argus dengan persen identifikasi sebesar 99,51 sampai dengan100%. Hasil penelitian menunjukkan bahwa antar sampel ikan kiper memiliki hubungan kekerabatan yang sangat dekat, namun populasi ikan kiper tergolong ke dalam kategori tidak baik karena tingkat keragaman yang rendah., Spotted scat (Scatophagus argus) is one of the demersal fish that has important economic value, this fish is used as a consumption fish and ornamental fish. It is also an active migratory fish that allows genetic transfer from different waters. This study aims to analyze the genetic diversity of Spotted scat in WPP 573 West Part based on 16s rRNA gene markers as a sustainable resource management. Amplification results show amplicon with single strand band but there are still smears due to the presence of contaminants. The BLASTn sequencing and validation results showed that all Spotted scat samples belonged to the Scatophagus argus species with a percent identification of 99.51 until100%. The results showed that the Spotted scat samples had a very close kinship relationship, but the population of spotted scat was classified into the unfavorable category because of the low level of diversity.
Keyword: Gen 16s, Genetic diversity, Scatophagus argus

Judul: Molecular identification of the indian mackerel (Rastrelliger  kanagurta) based on 16S rRNA gene markers in FMA NRI 572 and 573 as a basis  for fisheries management
Abstrak: Ikan kembung lelaki (Rastrelliger kanagurta) memiliki nilai ekonomis penting bagi nelayan. Ikan pelagis kecil di WPP-NRI 572 dan WPP-NRI 573 telah mengalami kondisi fully-exploited. Penelitian ini bertujuan memastikan spesies, keragaman genetik dan kekerabatan spesies secara molekuler ikan kembung lelaki (Rastrelliger kanagurta) berdasarkan marka gen 16S rRNA di WPP-NRI 572 dan 573 sebagai dasar pengelolaan perikanan. Penelitian dilakukan pada bulan Juni 2021 hingga Januari 2022. Sampel ikan diambil dari perairan Labuan, Cilacap, dan Banyuwangi dengan jumlah total sebanyak 9 individu. Urutan basa nukleotida hasil sekuensing dianalisis menggunakan software MEGA X. Hasil identifikasi DNA barcoding, menunjukkan spesies ikan kembung dari tiga lokasi berbeda merupakan ikan kembung lelaki (Rastrelliger kanagurta). Nilai keragaman genetik ikan kembung lelaki dari tiga lokasi termasuk dalam kategori tinggi. Hal ini menunjukkan populasi ikan kembung tergolong stok yang baik, The Indian Mackerel has important economic value for fishermen. The small pelagic fisheries in WPP-NRI 572 and WPP-NRI 573 have experienced a fully-exploited condition. This study aims to determine species certainty, genetic diversity and genetic relationships of indian mackerel (Rastrelliger kanagurta) molecularly based on 16S rRNA gene markers in WPP-NRI 572 and 573 as a basis for fisheries management. The research was conducted from June 2021 to January 2022. Fish samples taken from the waters of Labuan, Cilacap, and Banyuwangi with a total of 9 individuals. The nucleotide base sequences of the sequencing results were analyzed using MEGA X software. The identification results of DNA barcoding showed that the mackerel species studied from three different locations were the Indian Mackerel (Rastrelliger kanagurta). The results showed that the genetic diversity value of male mackerel from three locations was included in the high category indicating that the mackerel population was classified as a good stock.
Keyword: 16S rRNA genes, genetic diversity, Rastrelliger kanagurta

Judul: Sintesis dan Pencirian Natrium Titanat (Na2Ti2O4(OH)2) Berbahan Dasar Titanium Dioksida
Abstrak: TiO2 can be utilized as basic material for synthesis of sodium titanate (Na2Ti2O4(OH)2). This research was purposed to synthesize and characterize sodium titanate from TiO2 rutile powder as a basic material. Sodium titanate was synthesized by hydrothermal process at temperature 130 °C by mixing TiO2 rutile powder with 5 M and 10 M NaOH solution, then each was calcinated at temperature 700 °C. The energy dispersive X-ray analysis showed that sodium titanate has been successfully synthesized. This result was also supported by functional group analysis with Fourier transform infrared (FTIR) spectrofotometer showing the existence of Ti-O-Na vibration band at 989.42 cm-1. The formation of sodium titanate was depend on the reaction condition such as temperature, reaction time, and NaOH concentration. Scanning electron microscope image indicated that sodium titanate synthesized at 130 °C in 20 hours reaction time with 10 M NaOH had crystal size of 99.37 nm.
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