Judul: Analisis keragaman genetik labi-labi (Amyda cartilaginea) berdasarkan genom mitokondria
Abstrak: Amyda cartilaginea belongs to the Order Testudinata, Suborder Cryptodira and Family Trionychidae. Recently, the population of A. cartilaginea has been decreasing because of human exploitation and environmental destruction. Therefore, it is important to carry on the research in genetic diversity among this spesies. The research was conducted using the mitochondrial DNA marker by Polymerase Chain Reaction-Restriction Fragment Length Polymorphisms (PCR–RFLP) technique. PCR technique was conducted to observe genetic diversity of mtDNA using a pair of primer 577 and 578. The amplified region was control region which flanked tRNA Pro and tRNA Phe based on reference sequence of Dogania subplana. The amplification result ed in the product of 500 base pairs and 710 base pairs. The PCR product was then cut by restriction endonuclease, namely Hha I (GCG?C), HaeIII(GG?CC), AluI (AG?CT), MboI (?GATC) and DraI (TTT ?AAA). Twelve haplotypes was found among 39 samples.. Haplotype 2 showed wide distribution compared to other haplotypes. Nucleotide diversity among haplotypes is 5.4 %.
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Judul: Analisis keragaman genetik labi-labi, dogania Subpkma (Testudines: trionychidae), melalui teknik PCR-RFLP
Abstrak: Saat ini telah terjadi penurunan populasi labi-labi yang sebagian besar diakibatkan adanya perburuan liar dan kerusakan lingkungan. Melihat hal itu diperlukan suatu langkah untuk mengumpulkan berbagai informasi biologi labi-labi. Salah satu caranya adalah dengan melakukan anal isis molekular sebelum berbagai informasi lainnya diungkapkan. Penanda molekular genetik yang sering digunakan dalam analisis molekular adalah daerah pengendali (d-loop) genom mitokondria menggunakan teknik PCR-RFLP. Bagian yang paling stabil dari daerah pengendali mtDNA diamplifikasi secara in vitro dengan pasangan primer 577 dan 578. Produk PCR dipotong dengan lima macam enzim restriksi, yaitu Alu I, Hsp92 II, Hha I, Hpa II dan Rsa I yang menghasilkan tujuh haplotipe. Keragaman nukleotida antara haplotipe yang ditemukan di Jawa dan Sumatera (interlokasi) adalah 12.11 %. Angka itu jauh lebih besar dibanding keragaman nukleotida antar haplotipe di dalam Sumatera maupun Jawa (intralokasi) yang berturut-turut adalah 6.5% dan 3%.
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Judul: Pembuatan Film Tipis Cu2ZnSnS4 sebagai Lapisan Penyerap Cahaya pada Sel Surya Menggunakan Metode Elektrodeposisi
Abstrak: Thin film of Cu2ZnSnS4 (CZTS) is an alternative semiconductor used as light absorber layer on solar cell. CZTS can replace silicon that commonly used as commercial semiconductor. The advantage of using CZTS as absorber layer is due to the abundance of its chemical composer on earth, and its high value of absorption coefficient. The absorber layer was made by using electrodeposition method on ITO glass substrates. The films were deposited at room temperature using potentiostatic mode with a potential of –1.05 V for 30, 45, and 60 minutes. After that, the films were annealed at two different temperatures, 180 ºC and 500 °C, for 45 minutes in N2/H2S atmosphere. Based on X-ray diffraction analysis, the polycrystalline thin film obtained fall into kesterite crystal phase. This type of crystal was obtained when the CZTS was annealed at 500 °C in H2S/N2 atmosphere. The value of optical band gap energy for the CZTS thin film was 2.2 eV with the film thickness about 1.228–6.366 μm. The sample of CZTS with electrodeposition time of 45 minutes and annealing temperature of 500 °C showed the best result and in conformity with absorber layers criteria for solar cells.
Keyword: semiconductor, ITO glass, CZTS thin film, electrodeposition