Judul: Transformasi Tanaman Padi Dengan Gen Osdreb1a Melalui Agrobacterium Tumefaciens
Abstrak: Kekeringan, salinitas tinggi dan suhu rendah merupakan kendala abiotik yang dapat menurunkan produktivitas padi. Penggunaan varietas toleran kekeringan, salinitas tinggi dan suhu rendah merupakan alternatif yang tepat. Perakitan tanaman padi toleran kekeringan, salinitas tinggi dan suhu rendah dilakukan dengan transformasi gen OsDREB1A melalui Agrobacterium tumefaciens. Penelitian bertujuan untuk mendapatkan tanaman putatif transgenik padi Nipponbare dan IR64 yang mengandung gen OsDREB1A. Dari 410 embrio muda tanaman padi Nipponbare menghasilkan 40 (9,8%) tanaman putatif transgenik. Dari 40 tanaman putatif transgenik 28 (70%) tanaman transgenik positif mengandung gen OsDREB1A melalui analisis molekuler. Transformasi gen OsDREB1A pada 440 embrio muda tanaman padi IR64 belum berhasil mendapatkan tanaman putatif transgenik. Karakter agronomi menunjukkan bahwa 13 tanaman galur padi transgenik Nipponbare OsDREB1A generasi To mempunyai jumlah malai yang lebih banyak dibandingkan Nipponbare yang tidak mengandung OsDREB1A dan Nipponbare wild type yang ditumbuhkan dari biji. Sedangkan 6 tanaman galur padi transgenik Nipponbare OsDREB1A (To) mempunyai kisaran persentase gabah isi yang sama dengan Nipponbare wild type.
Keyword: analisis molekuler, gen OsDREB1A, padi, transformasi.

Judul: Karakterisasi Molekuler Tanaman Padi Nipponbare Transgenik 35S::OsERA1 yang Toleran Kekeringan
Abstrak: Drought is one of the factors that resulted in the growth and development of rice plants inhibited. One way to overcome this problem is use of transgenic rice plants that are tolerant to drought. The success of the transformation using OsERA1 gene in Nipponbare genotype rice requires further confirmation of tolerance to drought stress. The aim of this study was to obtain transgenic Nipponbare genotype rice contain gene 35S::OsERA1 drought tolerant, know the number of gene copies, and compare the contents and empty seeds with control. The evaluation of drought the tolerance was performed using Standard System Evaluation for Rice method (IRRI 1996). All tested transgenic genotypes were tolerant to drought. Molecular characterization using southern hybridization showed that transgenic rice plants had 1-3 copies of transgen. Drought stress inhibited plant growth, which lowers the amount of seed and increasing the void content. 18.5.4 and 6.4.10 genotypes are considered tolerant to drouht. 18.5.4 genotype have the number of grains per panicle contents of 36 seeds and 6.4.10 genotype have a 38.03% percentage of empty seeds
Keyword: transgenic plant, drought, southern hybridization, OsERA1 gene

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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