Judul: Pola penyebaran dan komposisi jenis mollusca dan crustecea di hutan Mangrove Kecamatan Muara Gembong - Bekasi
Abstrak: Karya Ilmiah ini merupakan hasil penelitian yang lakukan pada tanggal 12 April sampai dengan 12 Juni 1984. Tujuan penelitian untuk mengetahui pola penyebaran dan komposisi jenis macrofauna (mollusca dan crustacea) di hutan mangrove pantai timur Teluk Jakarta (Kecamatan Muara Gembong) serta hubungannya dengan faktor lingkungan. Kecamatan Muara Gembong terletak pada koordinat sekitar 106°57'30" 107°02'00" BT dan 05°54'00"06°03'00" LS. Daerah ini merupakan dataran rendah yang berada pada ketinggian 0-7 meter di atas permukaan air laut. Keadaan pantai berbentuk landai dan terdiri dari tanah berlempung (liat) berlanau dan lempung berpasir halus. Sepanjang garis pantai merupakan kawasan hutan mangrove (sebagian besar berupa semaian muda), juga banyak didapatkan muara. Tegakan hutan mangrove terdiri dari Avicennia spp., Sonneratia spp., dan Rhizophora spp., serta jenis-jenis lainnya. Pengambilan contoh menggunakan metoda "transek dan kuadrat", bingkai yang digunakan berukuran 0,5 x 0,5 m². Faktor lingkungan yang diamati adalah: suhu (udara dan air C tanah), salinitas (air tanah dan tanah), pH (air tanah dan dan tanah) serta tekstur tanah (persentase liat, debu dan pasir). Sedangkan faktor biotik yang diamati adalah jenis pohon mangrove dan fauna lainnya.
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Judul: Komposisi Jenis dan Struktur Vegetasi Hutan Mangrove di Suaka Margasatwa Muara Angke, Jakarta Utara
Abstrak: Hutan mangrove Suaka Margasatwa Muara Angke (SMMA) merupakan kawasan penyangga kehidupan di DKI Jakarta yang memerlukan penjagaan dari kerusakan. Informasi struktur dan komposisi jenis hutan mangrove dapat membantu pengelolaan berkelanjutan. Penelitian ini bertujuan mempelajari komposisi jenis dan struktur vegetasi hutan mangrove di SMMA. Analisis vegetasi dilakukan menggunakan metode grid dengan luasan yang diamati seluas 6.800 m2 berjumlah 17 petak dengan ukuran setiap petak 20 x 20 m. Penelitian ini menunjukkan bahwa hutan mangrove SMMA memiliki 26 jenis tumbuhan penyusun dari 21 famili. Jenis Sonneratia caseolaris dan Rhizophora mucronata mendominasi pada pohon dan permudaannya, Brachiaria mutica pada tumbuhan bawah, serta Nypa fruticans mendominasi palem-paleman. Nilai keanekaragaman dan indeks kekayaan jenis hutan mangrove SMMA tergolong rendah. Indeks dominansi hutan mangrove SMMA pada pohon dan palem tergolong tinggi, semai dan tumbuhan bawah tergolong sedang, dan pancang tergolong rendah, sedangkan kemerataan jenis pada pohon, pancang, dan tumbuhan bawah tergolong sedang, dan semai dan palem-paleman tergolong rendah. Struktur horizontal hutan mangrove membentuk kurva J terbalik, sedangkan struktur vertikal membentuk lapisan stratum C dan stratum D., Muara Angke Wildlife Reserve (SMMA) mangrove forest is the buffer zones for DKI Jakarta which requires protection from damage. Information of mangrove forest structure and species composition can assist sustainable management. This study aims to study species composition and vegetation structure of SMMA mangrove forest. Vegetation analysis was carried out by the grid method with an observed area of 6.800 m2 totaled 17 plots with a size of each quadrat 20 x 20 m. This study shows that the results of SMMA mangrove forest has 26 species from 21 families. Species Sonneratia caseolaris and Rhizophora mucronata dominate the level of trees and the regeneration, Brachiaria mutica of undergrowth, with Nypa fruticans dominate palms. The diversity and species richness index of SMMA mangrove forest was low. The dominance index on trees and palms were high, while seedlings and undergrowth were moderate, and on saplings were low, while the species evenness index on trees, saplings, and undergrowth was moderate, and for seedlings and palms low. For the horizontal structure of mangrove forest forms an inverted J-curve, while the vertical structure forms stratum C and stratum D.
Keyword: analisis vegetasi, hutan mangrove, Suaka Margasatwa Muara Angke

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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