Judul: Peranan Pemimpin Lokal dalam Meningkatkan Dinamika Kelompok Masyarakat di Pedesaan
Abstrak: Tujuan penelitian adalah untuk mengetahui: (1) peranan pemimpin lokal dalam meningkatkan dinamika kelompok, (2) ting- kat dinamika kelompok, ( 3 ) persepsi anggota kelompok terhadap pemimpin lokal , (4) karakteristik anggota kelompok, dan (5) hubungan antara peranan pemimpin lokal , persepsi anggota kelompok terhadap pemimpin lokal, karakteristik anggota kelompok, dan dinamika kelompok. Penelitian dilaksanakan di Desa Sumberwungu, Kecamatan Tepus, Kabupaten Gunung Kidul, Propinsi Daerah Istimewa Yogyakarta (DIY). Metode penelitian yang digunakan adalah metode kasus , sedangkan sifat penelitian adalah eksploratif dan verifikatif. Prosedur penelitian yang ditempuh adalah prosedur survai.
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Judul: Dinamika Politik dan Kepemimpinan Lokal, Pedesaan Jawa. Studi Kasus Desa Gadingsari, Kecamatan Sanden, Kabupaten Bantul, Provinsi Daerah Istimewa Yogyakarta.
Abstrak: Penelitian ini menggunakan konsep tentang politik dan kepemimpinan pedesaan sebagai bahan anaIisisnya Dinamika politik pedesaan dalam penelitian ini, erat kaitannya dengan proses demokrasi dan kehidupan politik yang terjadi. Kepemimpinan, terkait dengan kekuasaan dan hubungan di antara pemimpin pedesaan. Kehidupan politik dan kepemimpinan nantinya akan mampu memperlihatkan semangat demokratisasi dan otonomi desa yang ada
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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