Judul: Prediksi Potensi Cadangan Airtanah Menggunakan Persamaan Darcy di Kota Tangerang Selatan, Provinsi Banten
Abstrak: Water is very important for human beings. Sources of freshwater that can be used is only 3% of the total water availability on earth. One of fresh water’s sources that can be used is groundwater. The objective of this study was to determine the capacity of groundwater storages (discharge) in aquifers at South Tangerang city using the Darcy equation. The method of research was include several stages, such as the preparation phase, data collection, data processing, data analysis, and presentation of results. Data were obtained from geoelectric measurements using Schlumberger software, and were used to analyse the type and depth of rock layers. Beside, the data were also used to determined the position of confined and unconfined aquifer. The flownet of confined and unconfined aquifer indicated the scheme of groundwater’s flow and was used to decide the cross section of the flow. Darcy equation required another parameter called hydraulic conductivity. In this research, the aquifer was dominated by sand, so the hydraulic conductivity for sand (12 m/day) was used in calculation. As the result groundwater storages on unconfined aquifer was 3551,50 m3/day, while on confined aquifer was 16864.11 m3/day.
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Judul: Penentuan Sebaran Akuifer Dengan Metode Tahanan Jenis (Resistivity Method) Di Kota Tangerang Selatan, Provinsi Banten
Abstrak: Water is a basic requirement for humans being. The population growth can result a higher water demand. Surface water quality is declining due to human activities that make the usage of groundwater increased. The excessive exploration of groundwater can result soil subsidence, it is necessary to study the characteristics of groundwater. This study aimed to identify the lithology of soil, the position and thickness of aquifer, and to analyze aquifer distribution at the research area. The resistivity method was used to to identivity the lithology of soil. Analysis results showed that the depth of unconfined aquifer at the research area was 3,00-44,73 m below soil surface with a thickness of 2-12 m. Litology of soil was a clay and tufaan sand. The depth of confined aquifer was 80-130 m below soil surface with a thickness of more than 75 m. Groundwater flow patterns at the research location headed toward the north.
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Judul: Pemurnian dan pencirian protease serina dari ekstrak jamur shiitake (Lentinus edodes)
Abstrak: Shiitake (Lentimus edodes) adalah jamur untuk kesehatan yang rendah kalori, tinggi kandungan protein, kitin, zat besi, zink, serat, asam amino esensial, vitamin, dan mineral. Jamur shiitake telah digunakan lebih dari dua ribu tahun sebagai makanan dan obat tradisional di Jepang. Penelitian ini bertujuan memurnikan dan mencirikan protease serina dari ekstrak jamur shiitake. Ekstrak protease kasar diperoleh dengan cara ekstraksi jamur shiitake dalam 50 mM bufer universal pH 7. Ekstrak enzim dimurnikan dengan presipitasi amonium sulfat kejenuhan 70%, dialisis (cut off 10 kD), dan kromatografi penukar ion DEAE Sepharose. Pemurnian menghasilkan dua fraksi enzim aktif. Aktivitas spesifik tiap fraksi adalah 6,855 U/mg (fraksi 7) dan 10,098 U/mg (fraksi 19). Eluat DEAE memiliki tingkat kemurnian 1,89 dan 2,75 kali lebih tinggi terhadap ekstrak kasar dengan tingkat perolehan menurun hingga 0,4%. Hasil analisis SDS-PAGE 12% memperlihatkan fraksi protease terdiri atas 2 pita protein pada fraksi 7 (5) dan 47 kD) dan empat pita pada fraksi 19 (65, 51, 45, dan 37 kD). Aktivitas optimum protease dicapai pada subu 50°C dan pH 7. Uji zimogram 10% memperlihatkan bahwa protease mampu menghidrolisis substrat kasein, fibrinogen, albumin, dan gelatin dengan konsentrasi 1% (b/v). Aktivitas enzim protease kasar meningkat dengan adanya penambahan ion Na dan Fe (5 mM). Protease ekstrak kasar dihambat spesifik oleh inhibitor fenilmetilsulfonilklorida, N-p-tosil-L-lisin klorometilketon, dan soybean trypsine inhibitor sehingga digolongkan ke dalam kelompok protease serina serupa tripsin.
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