Judul: Water Footprint Analysis of Pocket Fertigation System for Cultivating Horticultural Plants in Open Land
Abstrak: Masalah efisiensi air masih menjadi isu penting dalam pertanian yang dapat direpresentasikan dengan indikator water footprint. Penghematan air dapat dilakukan dengan pengaplikasin sistem irigasi bawah permukaan, salah satunya sistem pocket fertigation. Tujuan penelitian ini menganalisis water footprint pada sistem pocket fertigation dengan berbagai skenario. Penelitian ini dilakukan di Kinjiro Farm pada waktu Maret – Juni 2023. Keempat skenario meliputi IK1 yang merupakan irigasi konvensional dengan irigasi satu kali evaporasi, IK2 yang merupakan irigasi konvensional dengan irigasi dua kali evaporasi, PF1 yang merupakan pocket fertigation dengan irigasi satu kali evaporasi, dan PF2 yang merupakan pocket fertigation dengan irigasi dua kali evaporasi, Hasil panen terbaik ditunjukkan PF1 dengan rata-rata produksi 13,38 ton/ha. Nilai water footprint terendah terjadi pada PF1 sebesar 540,37 m3/ton. Skenario PF2, IK1, dan IK2 memiliki water footprint berturut-turut 24%, 27%, dan 51% lebih besar dari water footprint PF1. PF1 menjadi skenario terbaik dikarenakan pemberian air yang langsung menuju perakaran dan jumlah irigasi mendekati kebutuhan air tanaman.
Keyword: evaporation, conventional irrigation, production, pocket fertigation, water footprint

Judul: Analisis Water Footprint pada Budidaya Pakcoy dengan Sistem Irigasi Bawah Permukaan Pocket Fertigation
Abstrak: Keterbatasan ketersediaan air dan penurunan produktivitas tanaman sawi khususnya pakcoy menyebabkan diperlukannya teknologi irigasi tepat guna. Pocket fertigation merupakan pengembangan dari sistem irigasi cincin yang diharapkan dapat meningkatkan produktivitas tanaman dan air. Penelitian ini bertujuan menganalisis water footprint tanaman pakcoy menggunakan sistem irigasi pocket fertigation denganberbagai skenario, serta menentukan sistem irigasi yang paling optimal. Penelitian dilaksanakan pada bulan April-Juni 2022 bertempat di Kinjiro Farm, Kota Bogor. Tahapan penelitian meliputi persiapan lahan, pengumpulan data, pengolahan data, dananalisis water footprint. Rancangan skenario irigasi dipengaruhi faktor sistem irigasi pocket fertigation (P1) dan konvensional (P2), serta pemberian air satu kali evaporasi (A1) dan dua kali evaporasi (A2). Total water footprint pada skenario P1A1, P2A1, P1A2, dan P2A2 secara berturut-turut sebesar 251,72 m3/ton, 231,56 m3/ton, 296,18 m3/ton, dan 222,53 m3/ton dengan produktivitas air tertinggi terjadi pada skenario P2A1, sehingga diperlukan pengembangan pocket fertigation lebih lanjut untuk meminimalkan water footprint dan meningkatkan produktivitas air., The limited availability of water and the decrease in the productivity of mustard plants, especially bok choy, have led to the need for appropriate irrigation technology. Pocket fertigation is the development of a ring irrigation system which is expected to increase crop and water productivity. This study aims to analyze the water footprint of bok choy plants using pocket fertigation irrigation system with various scenarios, and to determine the optimal irrigation system. The research was conducted in April-June 2022 at Kinjiro Farm, Bogor City. The research stages include land preparation, data collection, data processing, and water footprint analysis. The irrigation scenario design is influenced by the pocket fertigation (P1) and conventional irrigation systems (P2), as well as the provision of water one time evaporation (A1) and two times evaporation (A2). The total water footprint in the P1A1, P2A1, P1A2, and P2A2 scenarios, respectively, was 251,72 m3/ton, 231,56 m3/ton, 296,18 m3/ton, and 222,53 m3/ton with the highest water productivity occurs in the P2A1 scenario, so that further pocket fertigation is needed to minimize the water footprint and increase water productivity.
Keyword: bok choy, pocket fertigation, subsurface irrigation, water footprint

Judul: Mitochondrial DNA Gene Organization and Phylogenetic Relationship of Naja siamensis
Abstrak: Naja siamensis is one of Asiatic cobra in the Family of Elapidae, known as a cryptic snake. It has many morphological variations that lead to misidentification. Misidentification can cause the wrong concept in developing antivenom types due to high snakebite cases occur in Thailand. Thus, an accurate species identification using a molecular approach is crucially required. Mitochondrial DNA has been used widely in snake molecular studies. These studies were aimed to determine the arrangement of the N. siamensis partial mitochondrial genes using reptile-oriented primers and to reveal the phylogenetic relationship among other species of Asian cobra. DNA extraction was carried out based on the standard DNA salting-out protocol and was conducted by bioinformatics analysis using BLASTn and MEGA 6 software. Gene annotation was performed against N. kaouthia complete genome. A total of 3121 bp of N. siamensis mtDNA genes were sequenced consisting of partial of six regions: 16S rRNA, ND1, tRNA-Ile, CRII region, tRNA-Leu, tRNA-Gln, and COI genes. The mtDNA gene organization of N. siamensis was predicted to has a similar pattern with the common Elapidae mtDNA gene organization. This study showed that there is a monophyletic form in Naja genus and N. kaouthia is the closest species with N. siamensis based on partial 16S rRNA and ND1 gene.
Keyword: 16S rRNA, Elapidae, ND1, phylogenetic relationship