Judul: Analisis Keragaman Genetik Kunyit (Curcuma longa Linn.) Dan Temulawak (Curcuma xanthorrhiza Roxb.) Budidaya Tanah Jawa Berdasarkan Penanda Molekuler RAPD
Abstrak: Penanda molekuler RAPD (Random Amplified Polymorphic DNA) merupakan salah satu teknik yang digunakan dalam analisis keragaman genetik tanaman. Kunyit (Curcuma longa Linn.) dan temulawak (Curcuma xanthorrhiza Roxb.) sebagai tanaman obat herbal potensial memiliki distribusi luas di wilayah Indonesia yang dapat berpengaruh terhadap pola keragaman genetiknya. Penelitian ini bertujuan untuk mengetahui pola keragaman genetik dari 12 sampel genus Curcuma yang berasal dari berbagai daerah di Pulau Jawa. Penelitian diawali dengan optimasi isolasi DNA dan amplifikasi DNA, hingga analisis lokus dan konstruksi pohon filogenetik menggunakan metode UPGMA (Unweighted Pair Group Method Arithmatic Mean) dari software NTSYS. Modifikasi protokol isolasi DNA dengan penambahan Proteinase K setelah proses inkubasi dan RNAse setelah proses pencucian menunjukkan hasil paling baik. DNA yang diisolasi memiliki ukuran berkisar pada 9000 bp dengan kualitas pita yang cukup baik. Amplifikasi DNA dengan 20 primer acak terpilih pada 7 sampel kunyit menghasilkan 278 pita DNA dari 177 lokus, dan pada 5 sampel temulawak menghasilkan 184 pita DNA dari 143 lokus yang seluruhnya merupakan pita polimorfik. Analisis kemiripan genetik 7 varietas Curcuma longa Linn. menunjukkan nilai koefisien kemiripan genetik sebesar 42.37% - 94.35% dan 19.58% - 85.31% pada 5 varietas Curcuma xanthorrhhiza Roxb. Hasil pengelompokan pada nilai koefisien kemiripan genetik 51.65% dan 33.17% menunjukkan bahwa 7 varietas kunyit dan 5 varietas temulawak yang diteliti berasal dari dua kelompok besar.
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Judul: Pola Keragaman Genetik Tanaman Kunyit (Curcuma longa Linn.) dan Temulawak (Curcuma xanthorrhiza Roxb.) Asal Pulau Jawa dengan PCR-RAPD
Abstrak: Turmeric (Curcuma longa Linn.) and Temulawak (Curcuma xanthorrhiza Roxb.) are Indonesian medicinal plants which nutritious for improving health. The pattern of genetic diversity and genetic relationship of these rhizome plants are not well known yet. The purpose of this research to analyze the pattern of genetic diversity and genetic relationship both plants using Polymerase Chain Reaction- Random Amplified Polymorhic DNA (PCR-RAPD). The sample used in this research include 6 turmeric plants from Bogor, Wonogiri, Karanganyar, Sukabumi and Ngawi; and 5 temulawak plants from Bogor, Wonogiri, Sragen, Karanganyar and Sukabumi. Stage of these research include DNA isolation; DNA amplification; and analysis pattern of genetic diversity and genetic relationship using NTSYS 2.02. The result of these research showed that isolation DNA was done successfully. The result of DNA isolation generally has a fairly good purity. The purity values of turmeric and temulawak in sequence were 1.093-1.417 and 1.044-1.544. The number of bands that arise from DNA amplification of turmeric and temulawak in sequence were 131 and 132 locus. The number of amplification bands mixed results and different sizes for each sample show the patterns of genetic diversity found in turmeric and temulawak plants. The closest genetic relationship showed by turmeric plants from Sukabumi and Ngawi; and temulawak plants from Bogor and Sukabumi. Genetic similarity coefficient ranged from turmeric plants and temulawak plants in sequence are 54%-92% and 55 %-83 %.
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Judul: Kekonsistenan Hamiltonian pada Gerak Gelombang Interfacial
Abstrak: The interfacial wave is an internal wave that occurs at boundary of two layers fluid. It appears because there is difference of mass density between the two layers. The objective of this thesis is to formulate the motion of internal wave with flat base and flat as well as free upper bound surface. The motion equation obtained is a Hamiltonian system. The Hamiltonian in this system is expressed by variable of wave deviation and horizontal component of potential velocity. Under assumption that interfacial wave moves only in one direction, it can be shown that the Hamiltonian depends on the solution of the wave motion equation. The coefficients of the equation depend on the physical characteristics of the two layers of fluid, i.e. thickness and mass density. The numerical results show that Hamiltonian in the system is constant. It is consistent with Hamiltonian characteristic, which does not depend on time.
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