Judul: Studi mutu sekolah melalui nem SMA sekotamadya Bandung
Abstrak: Penelitian ini mengkaji nilai-nilai bidang studi Ebtanas untuk melihat peranannya sebagai indikator mutu sekolah. Dari hasil analisis biplot diperoleh bahwa komponen pertama merupakan indikator prestasi sekolah. secara umum, sedangkan Komponen kedua menunjukkan prestasi sekolah pada pelajaran-pelajaran pokok masing-masing jurusan. Selain itu sekolah-sekolah dapat dikelompokkan menjadi tiga kelompok, yaitu sekolah-sekolah yang terletak di sebelah kiri titik asal dianggap memiliki prestasi rendah, sekolah-sekolah yang terletak di sebelah kanan tetapi tidak jauh dari titik asal adalah kelompok sekolah dengan prestasi sedang; sedangkan sekolah-sekolah dengan prestasi baik terletak jauh di sebelah kanan titik asal. Masing-masing sekolah berprestasi baik ini ialah SMAS Santo Aloysius 1 dan 2, SMAS BPK 1, dan SMAN Bandung 3.
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Judul: Nilai rapor, tes aptitud, dan nem SMP : studi kasus di SMA Kornita dan SMA Negeri 1 Bogor
Abstrak: Tujuan penelitian ini adalah menelaah hubungan antara nilai rapor, tes aptitud, dan nilai EBTA murni SMP, hubungan antar mata pelajaran (nilai rapor) dan hubungan antar tes aptitud. Juga untuk melihat keberadaan hasil tes aptitud dan keberadaan hasil penjurusan dalam peubah penentu penjurusan. Data diperoleh dari dokumentasi pada SMA Kornita dan SMA Negeri 1 Bogor. Dengan obyek pengamatan siswa SMA Negeri 1 angkatan tahun 1985/1986 dan siswa SMA Kornita angkatan tahun 1986/1987. Hasil analisis korelasi menunjukkan bahwa tes aptitud ada berhubungan dengan nilai rapor dan NEM SMP. pula untuk NEM SMP dengan nilai rapor. Demikian Berdasarkan penggambaran biplot didapatkan bahwa siswa A1 secara rata-rata memiliki skor tertinggi baik dalam prestasi di sekolah (nilai rapor), kemampuan potensial maupun NEM SMP. Siswa A2 berada dibawah skor siswa Al, sedang siswa A3 memiliki skor terendah. Hasil lain menunjukkan bahwa arah vektor peubah mata pelajaran IPA (Biologi, Fisika, Kimia dan Matematika) cenderung menyatu. Bahasa Inggris muncul dengan arah yang berlainan dan sangat berperan dalam menyusun komponen utama kedua.
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Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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