Judul: Studi interaksi Fusarium sp. dengan pohon gaharu (Aquilaria sp.) menggunakan pendekatan sitologi
Abstrak: The research is aiming to study the pathogenesis mechanism of Fusarium sp. IPBCC. 08.569 on Aquilaria sp. seedling in respect to the respons of the host towards Fusarium sp. IPBCC. 08.569 infection. Penetration mechanism was determined by observing the appresorium formation on the bark surface in vitro, the potency to produce lignocellulase and toxin. While colonization was studied by histopathological methods using both staining process and SEM. The respons of the tree towards infection was observed at either cellular by histochemestry or organ level using scoring system. Fusarium sp. IPBCC. 08.569 showed no appresorium formation but had lignocellulolytic activity. Hypersensitivity reaction of tobacco leaf towards crude extract of extracellular substance indicated that Fusarium sp. IPBCC. 08.569 produce toxin. Direct penetration into the bark was not found, therefore penetration had to be through wounded area. While modification of hyphae was observed in the living cells of the wood tissue (parenchyma rays, included phloem, and pith) these cells might contain terpenoid as conversion of starch. These cellular changes might be observed as discolored wood and the wood became scented. Discolored wood was extended outside the colonization area, indicated that the dark substance might be part of hypersensitive respons to delimit the infection. Using scoring system, after inoculation Fusarium sp. IPBCC. 08.569 can be stated as wound infection fungi with limited colonization in living tissue and induce terpenoid formation in certain tissue.
Keyword: 

Judul: Karakterisasi interaksi antara tanaman aquilaria microcarpa baill dengan fusarium solani dalam pembentukan gaharu
Abstrak: Aquilaria microcarpa is native to Indonesia and has been identified and promoted as potential tree species to yield high quality agarwood (gaharu). Individual trees of this species, both in its natural habitat and plantation, do not always yield agarwood due to many influencing factors. It is estimated that only 10% trees would be able to yield the resin. A clear mechanism of agarwood formation is unknown and there is a lack of information on underlying causes and triggers of the formation due to natural complexity and uncertainty. However, natural defense mechanisms through a series of pathogenesis are presumed as naturally observed in other resin producing tree species. Research on the characterization of host-pathogen interaction in gaharu formation was carried out based on the above mentioned problems with the following specific objectives, namely: i). to identify morphological characters of A. microcarpa trees interacting with F. solani sp, ii). to determine wood anatomical characters of A. microcarpa interacting with F. solani, and iii) to verify the genotypes of A. microcarpa seedlings and trees interacting with F.solani based on microsatellites. Results on tree morphological analysis showed that two out of 17 morphological characters (descriptor), namely tree height (m) and branching angles (0), showed significant differences between inoculated and non-inoculated individuals. In addition, microscopic observations on wood anatomical characters of A. microcarpa revealed that inoculated plants clearly deposited golden-brown colored resin in pores and canticles with a distinct scent. Further verification using GC MS on the sediment deposits identified common compounds as reported in many previous research on agarwood, namely: baimuxinal, elemol, 3-phenyl-2-butanone and chromone-3-one. On the other hand, tests on the viulence degrees of F. solani showed that one out of 4 strains, i.e. F.solani FORDA 512, showed the highest level of virulence and was significantly different with other F.solani, especially if inoculated on seedlings A60, A68 and B30. Two microsatellite loci of A. crassna, i.e. 6Pa18 and 71Pa17, were successfully transferable to A. microcarpa and could be used to characterize the genotypes of Fusarium-treated seedlings (n=40), inoculated trees (n=20) and non-inoculated trees (n=20). There were differences in terms of genetic structures between tree population and seedling population. Further sequence analysis on the microsatellite fragments on the most virulent F.solani FORDA 512- inoculated seedlings indicated genotype dependant responses for both seedling and tree stages. In addition, Sequence based cladogram showed that inoculated seedlings were closely placed with inoculated trees indicating certain genetic roles in the agarwood formation. In conclusion, tree morphological (i.e. height growth and branch habit) as well as genetic and genomic characters were clearly observed and useful to provide early information on gaharu formation. It is also supported by the wood anatomy analysis showing the presence of gaharu resin.
Keyword: 

Judul: Konsumsi Pangan dan Status Gizi Ibu Hamil di Kecamatan Danurejan, Kodya Yogyakarta, Daerah Istimewa Yogyakarta
Abstrak: Hasil penelitian menunjukkan bahwa rata-rata konsumsi energi per orang per hari adalah 1 525 Kalori, konsumsi protein 45,l g, konsumsi zat besi 12,8 mg dan konsumsi vitamin A 2 264 SI. Rata-rata kecukupan energi per orang per hari adalah 2 213 Kalori, kecukupan protein 75,6 g, kecukupan zat besi 29,O mg dan kecukupan vitamin A 4 500 SI.
Keyword: