Judul: Identification of Rice Aromatic Properties Using Molecular Aromatic Gene-based Marker.
Abstrak: Rice as a basic commodity can be distinguished between aromatic and nonaromatic. Its different based on gene deletion at chromosom 8. Conventional methods like assessment was method which frequently uses to detect aromatic characteristic of rice. Molecular gene based marker was new method to identify aromatic rice accurately and shorthen analyzing time. The purpose of this research is to distinguish the type of aromatic and nonaromatic variety using aromatic gene-based marker. DNA as rice genetic material was isolated from leaves using CTAB method which broke the cell wall and release the components thus precipitated the DNA. DNA amplification conducted by PCR machine using specific aromatic primer and the result verified by 1.5 % gel agarose electrophoresis. The result of Bradbury primer obtained size of band about 580 bp and 355 bp for nonaromatic, also 580 bp and 257 bp for aromatic. Mentik Wangi and Gunung Perak variety could distinguish to band pattern. Furthermore, RM223 primer can be differentiate both of patterns of aromatic and nonaromatic rice by the band size about 120 bp to 160 bp.
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Judul: Aplikasi Marka Aromatik Bradbury dan RM 223 untuk Identifikasi Hasil Persilangan Ciherang-Mentik Wangi dan Ciherang-Pandan Wangi.
Abstrak: Rice as a basic commodity can be distinguished between aromatic and nonaromatic. Its different based on gene deletion at chromosom 8. Conventional methods like assessment was frequently uses to detect aromatic characteristic of rice. Gene based molecular was a new method to identify aromatic rice accurately and shorthen analyzing time. The purpose of this research were to select and to get rice F1 Ciherang-Mentik Wangi use Bradbury primer and BC1F1 Ciherang- Pandan Wangi use RM223 primer. DNA as rice genetic material were isolated from leaves using Cetil Trimetil Amonium Bromida (CTAB) method which broke the cell wall and release the components, thus precipitated the DNA. DNA amplification conducted by PCR machine using specific aromatic primer and the result verified by 1.5 % and 3% gel agarose electrophoresis. The result of amplification by using Bradbury primer of F1 Ciherang-Mentik Wangi obtained size of band about 580 bp and 355 bp for nonaromatic, also 580 bp and 257 bp for aromatic. Furthermore, RM223 primer can be differentiate both of patterns of aromatic and nonaromatic rice by the band size about 120 bp to 160 bp.
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Judul: Fasciloliasis Pada Hewan Ternak Di Indonesia
Abstrak: Fascioliasis adalah salah satu penyakit hewan yang sangat merugikan yang disebabkan infeksi cacing hati (Fasciola sp.). Cacing hati termasuk kelas Trematoda dari famili Fasciolidae, bentuknya pipih seperti daun de- ngan ukuran bervariasi. Cacing ini termasuk hewan hermoprodit dimana alat kelamin jantan dan betina berada dalam satu tubuh. Di Indonesia hanya ditemukan satu spesies cacing hati yang menyerang hewan ternak yaitu Fasciola gigantica (Mukhlis, 1984 komunikasi pribadi). Siklus hidup cacing hati memerlukan siput sebagai induk semang antaranya. Di Indonesia siput ini ialah Lymnaea rubiginosa. Menurut Hall (1977) cacing hati yang sudah dewasa di dalam pembuluh empedu induk semang utama akan bertelur, tiap ekor cacing dapat menghasilkan 3000-3500 telur/hari. Fascioliasis dapat bersifat akut atau kronis tergan tung faktor umur, jumlah cacing yang menginfeksi dan jenis hewan ternak. Pada domba penyakit ini lebih sering bersifat akut, sedang pada sapi biasanya bersifat kronis. Pada keadaan akut biasanya hewan penderita tiba-tiba mati tanpa menimbulkan gejala klinis, bila ada gejalanya terlihat hanya sebentar. Gejala klinis pada bentuk akut yaitu lemah, nafsu makan berkurang, mudah lelah bila ber jalan dan sikapnya berbaring dengan bagian dada serta hidung kena ke tanah (Taylor, 1964). ...
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