Judul: Identifikasi Gen Xa pada Galur F1 Code x Angke dan Ciherang x IRBB21 serta Uji Patogenitas Padi Galur Isogenik
Abstrak: Developing of Ciherang varieties using bacterial leaf blight resistance genes (Xa genes) and pathogenicity tests using bacterial isolate Xanthomonas oryzae pv. oryzae collections in 2012 from Central Java toward near-isogenic lines (NILs) have not been reported. Transfer of resistance genes in Ciherang varieties did by cross method and molecular marker. Varieties of Angke crossed with Code because each has a Xa gene, namely xa5 and Xa7 gene. While Ciherang crossed with IRBB21 as Xa21 gene donor. Identification of xa5 and Xa7 genes in Code x Angke were resulted 16 lines have those genes. xa5 gene amplified in 213 bp using SSR marker, while Xa7 gene amplified in 294 bp using STS marker. These DNA size are resistant marker. Identification of Xa21 gene in Ciherang x IRBB21 was resulted 21 lines have this gene. Xa21 gene amplified in 1000 bp using STS marker as resistant marker. The result of pathogenicity tests obtained bacterial isolate whose have highest levels of virulence, that is isolate with the code number Xoo12-183 from Duwet Pekalongan.
Keyword: Xa gene, pathogenicity, paddy Ciherang, near-isogenic lines (NILs), molecular marker

Judul: Identifikasi Xanthomonas oryzae pv. oryzae dengan Teknik Biomolekuler dan Karakter Patogenitas terhadap Padi Galur Isogenik.
Abstrak: Increased production of paddy can face obstacle because the biotic stresses like bacterial leaf blight (BLB). The aim of this research to identification BLB caused by Xanthomonas oryzae pv oryzae (Xoo) by biomolecular technique using three primers specific Xoo through PCR colony, and to know virulence of Xoo isolates from West Java, West Sumatera, West Borneo by clip method for character of pathogenicity analysis using near-Isogenic lines and popular varieties. The purifying result were obtained 72 isolates namely 20 isolates from West Java, 30 isolates from West Sumatera, 22 isolates from West Borneo. Most of isolates were identified Xoo positive, except isolate Xoo11-023 from West Sumatera were identified Xoo negative. The character of pathogenicity showed that Code which contain resistant gene Xa4-Xa7 and IRBB5 which contain resistant gene xa5 were resistant to Xoo from all three areas. The average disease intensity on Code and IRBB5 of isolates from West Java was 1.62 % and 3.55%, West Sumatera was 2.10 % and 2.97 %, West Borneo was 2.17 % and 4.45 %. Isolate Xoo11-042 from West Sumatera was the one having highest virulence with average disease intensity on Code 3.29 % and IRBB5 6.90 % which classified as moderate resistant.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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