Judul: Karakterisasi Gen Ghrelin (GHRL) pada Ayam Kampung Ayam Leher Gundul Ayam Walik dan Ayam Arab
Abstrak: Ghrelin gene (GHRL) is one of important gene that stimulates secretion of gastric acid, growth hormone and food intake. Sekuens of GHRL fragment in this study was located in promoter, exon 1 and partial intron 1. This study aimed to characterize GHRL gene especially to detect mutation sites of kampong, naked neck, walik, and arab chicken. Twenty samples were amplified to result of fragment with length of 1 050 bp. Characterization of GHRL gene was processed by BLAST-N, then MEGA 5 was used to analize the sequence data. Annealing temperature was performed at 60 ºC for 1 minute. The alignment product of eight samples by using primer forward produced sequence of 554 bp. The similarity value of GHRL gene fragment with sequence data on GenBank (AB158617) was 99%. There was found 3 mutation sites of GHRL gene fragment. However, the 554 bp of GHRL gene fragment has low power to distinguish chicken breeds since all the samples have pairwise distance based on the phylogenetic tree construction analysis.
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Judul: Identifikasi Keragaman Gen Growth Hormone (GH|EcoRV) pada Ayam Kampung di Indonesia Menggunakan Metode PCR-RFLP.
Abstrak: Growth hormone (GH) dikenal sebagai salah satu hormon pengontrol pertumbuhan makhluk hidup termasuk produksi telur, pertumbuhan otot, dan pertumbuhan tulang. Tujuan penelitian ini adalah mengidentifikasi keragaman gen GH yang terdapat pada ayam kampung dengan metode Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Keragaman gen GH dianalisa dari 106 ekor ayam kampung yang terdiri dari 49 ekor ayam kampung Ciawi dan 57 ekor ayam kampung Sukabumi, sebagai data pembanding digunakan juga 20 ayam Cobb, 10 Isa Brown, dan 10 ayam kampung x Isa Brown. Gen diamplifikasi dengan suhu annealing 60 oC selama 20 detik dengan panjang sekuen 339 pb yang terletak pada intron 3. Hasil amplifikasi dipotong menggunakan enzim EcoRV dengan suhu 37 oC. Data yang diambil adalah frekuensi genotipe, frekuensi alel, heterozigositas, dan keseimbangan Hardy- Weinberg. Hasil penelitian menunjukkan alel G lebih besar dari alel A. Berdasarkan hasil dapat disimpulkan bahwa ayam kampung, Cobb, dan Isa Brown bersifat polimorfik sedangkan ayam kampung x Isa Brown bersifat monomorfik.
Keyword: ayam kampung, gen GH, keragaman, PCR-RFLP

Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4