Judul: Persepsi dan perilaku mahasiswa tentang pergaulan lawan jenis
Abstrak: Secara urnurn tujuan dari penelitian ini adalah untuk mengetahui persepsi contuh tentang pergaulan lawan jenis dan perilaku pergaulan lawan jenis contoh. Sedangkan tujuan khususnya, yeitu : (I)M engetahui karakteristik contoh, karakteristik keluarga contoh dart karakteristik lingkungan '(perasaan contoh terhadap permasalahan di kampus); (2) Mengetahui tujuan hidup contoh, persepsi contoh tentang pergaulan lawan jenis dan perilaku contoh tentang pergaulan lawan jenis, dan tingkat kepuasan hidup contoh; (3) Mengetahui hubungan antar variabel (karakteristik contoh dengan karakeristik keluarga dan karakteristik lingkungan; karakteristik keluarga dengan karakteristik lingkungan; karakteristik contoh, karakteristik keluarga dan karaheristik lingkungan dengan tujuan hidup, pemepsi tentang pergaulan lawan jenis, perilaku pergaulan tawan jenis dan tingkat kepuasan hidup; tujuan hldup dengan persepsi tentang pergaulan lawan jenis dan perilaku pergaulan tawan jenis; persepsi tentang pergaulan lawan jenis dengan perilaku pergaulan fawan jenis den tingkat kepuasan hidup; dan perilaku pergaulan lawan jenls dengan tingkat kepuasan hidup); (4) Menganalisis faktor-faktor yang berpengaruh terhadap persepsi contoh terhadap pergaulan lawan jenis dan perilaku pergaufan lawan jenis contoh.
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Judul: Persepsi Identitas Gender dan Konsep Diri Tentang Peranan Gender di Kalangan Mahasiswa (Kasus Mahasiswa Tingkat Persiapan Bersama Institut Pertanian Bogor Tahun Ajaran 2007/2008)
Abstrak: Ketidaksetaraan antara perempuan dan laki-laki dalam berbagai dimensi kehidupan, termasuk pendidikan, diduga berhubungan dengan berbagai faktor, diantaranya ideologi gender yang terinternalisasi pada hampir semua masyarakat. Mahasiswa sebagai generasi muda nantinya akan mengisi posisi-posisi strategis dalam kelembagaan masyarakat Indonesia di masa depan, sehingga persepsi dan konsep diri mahasiswa mengenai peranan gender dalam keluarga, masyarakat, dan bernegara serta faktor-faktor yang mempengaruhinya akan menentukan keberhasilan pembangunan dalam mewujudkan KKG dalam beragam dimensi kehidupan. Tujuan penelitian ini adalah untuk mempelajari mengenai : (1) persepsi identitas gender -maskulin, feminin, dan androgini- yang terinternalisasi pada mahasiswa TPB IPB, serta untuk mengetahui faktor-faktor yang mempengaruhinya, (2) Peranan gender -produktif, reproduktif, dan pengelolaan masyarakat dan politik- yang menjadi bagian dari konsep diri mahasiswa TPB IPB, (3) Agen sosialisasi gender di kalangan mahasiswa yang berperan mempengaruhi pembentukan identitas gender dan konsep diri peranan gender mahasiswa TPB IPB, dan (4) Stereotipe gender di kalangan mahasiswa TPB IPB serta harapan atas peranan gender mereka, khususnya ketika mereka akan memilih pasangan hidup dalam membentuk keluarga inti.
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Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable