Judul: Sikap Masyarakat Kelurahan Pancoran Mas Terhadap Taman Hutan Raya Pancoran Mas, Depok.
Abstrak: Pancoran Mas Grand Forest Park is Located among residences, resulting interaction between grand forest park and the community. Today, only with a very bad repair iron fence that surrounding the park. This site had been neglected for several yaers. The main problem now is the management of this site. The effectivenes of grand foret park management depend on the recognition and support of the community living around the park. Knoweledge, attitudes and behaviour of the people will affect the grand forest park existence and conditions. Attitudes is a component that forms behaviour. This research is intended to measure the attitude of the people towards Pancoran Mas Grand Forest Park
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Judul: Public Perception toward the Status Change from a Natural Reserve Area to Grand Forest Park of Pancoran Mas in Depok
Abstrak: Taman Hutan Raya adalah kawasan pelestarian alam untuk tujuan koleksi tumbuhan dan atau satwa yang alami atau buatan, jenis asli dan atau bukan asli, yang dimanfaatkan bagi kepentingan penelitian, ilmu pengetahuan, pendidikan, menunjang budidaya, budaya, pariwisata dan rekreasi. Cagar Alam yang merupakan status awal kawasan membuat fungsi kawasan ini seolah-olah tidak terkelola. Padahal saat ini fungsinya telah diubah menjadi Taman Hutan Raya, dimana kawasan ini bisa digunakan untuk kepentingan wisata sesuai pembagian blok pengelolaannya. Penelitian ini bertujuan untuk memahami persepsi masyarakat terhadap perubahan status kawasan Cagar Alam menjadi kawasan Taman Hutan Raya Pancoran Mas Depok dan mengidentifikasi faktor-faktor yang mempengaruhi persepsi masyarakat tersebut. Penelitian ini dilaksanakan pada bulan April sampai Mei 2012 dengan pemilihan responden menggunakan metode purposive sampling. Responden yang diambil, yaitu: laki-laki 26 responden dan perempuan 26 responden di Kelurahan Pancoran Mas, Depok. Faktor-faktor yang mempengaruhi persepsi dianalisis dengan menggunakan korelasi Rank Spearman dengan SPSS 16.0.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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