Judul: Validation of Microsatellite Molecular Markers on Matoa (Pometia pinnata)
Abstrak: Indonesia adalah negara yang kaya akan keanekaragaman hayati dengan berbagai jenis spesies tumbuh-tumbuhan. Keanekaragaman tersebut terdiri dari tanaman buah yang salah satunya berasal dari buah Matoa (Pometia pinnata). Matoa merupakan tanaman dari famili Sapindaceae yang tersebar di wilayah tropis. Tanaman matoa banyak dimanfaatkan sebagai obat-obatan tradisional yang diketahui mengandung kelompok senyawa diantaranya flavonoid, tanin, dan saponin. Analisis keragaman genetik matoa menjadi informasi dasar untuk pelaksanaan kegiatan seleksi dan persilangan lanjutan dalam program pemuliaan matoa. Marka mikrosatelit merupakan salah satu metode analisis marka molekuler kodominan. Tujuan dari penelitian ini untuk melakukan seleksi primer untuk analisis kekerabatan 10 aksesi matoa Dramaga dengan 4 matoa Banjarmasin melalui pendekatan marka mikrosatelit. DNA diisolasi menggunakan CTAB (Cetyl Trimethyl Ammonium Bromide). Sepuluh primer mikrosatelit yang digunakan adalah Pp.e.6402, Pp.e.66857, Pp.e.80271, Pp.e.89451, Pp.e.115726, Pp.e.161167, Pp.e.186462, Pp.e.187105, Pp.e.238124, Pp.e.287861. Amplifikasi mengacu pada protokol DreamTaq Green PCR Master Mix. Hasil penelitian menunjukkan bahwa primer yang digunakan mampu mengamplifikasi 20-30% aksesi yang digunakan, dan hanya 2 aksesi yang dapat diamplifikasi pada 9 pasang primer yang digunakan, yaitu aksesi IPBA8, IPBA9. Sembilan primer dapat digunakan untuk analisis molekuler, kecuali primer Pp.e.287861 yang tidak mampu mengamplifikasikan 14 aksesi yang digunakan., Indonesia is a country rich in biodiversity with various types of plant species. One of the diversity of fruit plants comes from the Matoa fruit plant (Pometia pinnata). Matoa is a plant from the Sapindaceae family which is spread throughout the tropics. The matoa plant is widely used as a traditional medicine which is known to contain a group of compounds including flavonoids, tannins and saponins. Analysis of matoa genetic diversity is the basic information for implementing selection and crossbreeding activities in matoa breeding programs. Microsatellite markers are one of the codominant molecular marker analysis methods. This study aims to conduct primary selection for kinship analysis of 10 Dramaga matoa accessions with 4 Banjarmasin matoa using a microsatellite marker approach. DNA was isolated using the CTAB (Cetyl Trimethyl Ammonium Bromide). The ten microsatellite primers used were Pp.e.6402, Pp.e.66857, Pp.e.80271, Pp.e.89451, Pp.e.115726, Pp.e.161167, Pp.e.186462, Pp.e.187105, Pp.e.238124, Pp.e.287861. SSR marker analysis using PCR DreamTaq Green Master Mix. The results showed that the primers used were able to amplify 20- 30% of the accessions used, and only 2 accessions could be amplified in the 9 pairs of primers used, namely IPBA8, IPBA9 accessions. Nine primers could be used for molecular analysis, except for the primer Pp.e.287861 which was unable to amplify the 14 accessions used.
Keyword: allele, biodiversity, codominant, fruit plants, genetic

Judul: Validation of Microsatellite Molecular Markers on Several Santol Plants (Sandoricum koetjape Merr.)
Abstrak: Tanaman kecapi tergolong dalam famili Meliaceae yang banyak dibudidayakan di Asia Tenggara, terutama di Indonesia dan Thailand. Aksesi tanaman kecapi sulit dibedakan secara morfologi, sehingga perlu dilakukan pendekatan berbasis molekuler menggunakan marka mikrosatelit. Penelitian ini dilakukan di Laboratorium Seed Center Departemen Agronomi dan Hortikultura Institut Pertanian Bogor pada bulan September 2022 hingga Mei 2023. Pada penelitian ini digunakan 16 aksesi tanaman kecapi masing-masing dari lokasi yang berbeda. Uji kuantitas dan kualitas DNA 16 aksesi kecapi menggunakan spektrofotometer dihasilkan konsentrasi DNA berkisar antara 100,6 hingga 786,6 ng/µL. Kemurnian DNA berkisar antara 1,41 hingga 2,14 pada A260/A280 dan 0,38 hingga 1,97 pada A260/A230. DNA masing-masing aksesi diamplifikasi menggunakan 10 primer mikrosatelit, namun hanya aksesi BGR5, BNT, IPB3 yang dapat teramplifikasi. Hal tersebut menunjukkan primer Sk1 hingga Sk10 masih perlu divalidasi., The santol plant belongs to the Meliaceae family which is widely cultivated in Southeast Asia, especially in Indonesia and Thailand. It is difficult to distinguish morphologically between santol plant accessions, so it is necessary to do a molecular-based approach using microsatellite markers. This research was conducted at the Seed Center Laboratory of the Department of Agronomy and Horticulture, Bogor Agricultural University from September 2022 to May 2023. In this study, 16 accessions of the santol plant were used, each from a different location. Testing the quantity and quality of DNA of 16 santol accessions using a spectrophotometer resulted in DNA concentrations ranging from 100.6 to 786.6 ng/µL. DNA purity ranged from 1.41 to 2.14 for A260/A280 and 0.38 to 1.97 for A260/A230. The DNA of each accession was amplified using 10 microsatellite primers, but only the BGR5, BNT, IPB3 accessions could be amplified. This shows that the primers Sk1 to Sk10 still need to be validated.
Keyword: Santol, microsatellite, amplification, genetic

Judul: Dynamic New Keynesian Model for Unemployment and Inflation Rate.
Abstrak: This paper deals with a scientific analysis of the equilibrium level of hiring, employment and unemployment. The basic model being used is a standard new Keynesian model, which states that utility function depends on consumptions and working hours. Utility maximization refers to a standard labour supply equation, with the marginal cost’s dynamics on new Keynesian model simply reflects movements in real wages and productivity. Furthermore, trade-off between output and inflation stabilization can be considered using the new Keynesian Phillips curve. Dynamic general equilibrium model is a model that can be used to study interactions between market imperfections and nominal rigidities, which is capable of overcoming the main shortcoming of the standard new Keynesian model. In the general equilibrium model there are three groups of the economy agents, namely households, firms and monetary authority. The results of this research science that bargaining processes between firms and workers will yield optimal wage. Increase in labour will cause wages to fall, but the unemployment rate can be reduced. An increase in the firm’s monopoly power will shift the labour demand curve downward, which will reduce the number of workers that firm is willing to hire for any given bargained wage.
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