Judul: Analisis Topik Data Media Sosial Twitter Menggunakan Model Topik Latent Dirichlet Allocation
Abstrak: Media sosial merupakan sebuah platform yang penggunanya dapat berbagi pandangan, ide, perasaan, dan emosi mengenai suatu konteks peristiwa atau hal yang sedang terjadi saat itu. Dengan adanya media sosial Twitter, para pengguna dapat menyampaikan pendapatnya dengan mengikuti isu terkini yang sedang berkembang. Penelitian ini bertujuan untuk melakukan analisis topik menggunakan metode pemodelan topik Latent Dirichlet Allocation (LDA) sehingga diperoleh topik utama yang sedang ramai diperbincangkan. Pemodelan topik menghasilkan distribusi kata yang membentuk topik dan kumpulan dokumen dengan topik tertentu. Jumlah topik yang digunakan berbeda untuk masing-masing data yang diolah, berdasarkan pada konteks dari kumpulan tweet yang diakuisisi. Pemodelan topik menggunakan parameter yang sama untuk semua data kecuali parameter jumlah topik. Analisis topik dilakukan berdasarkan data keluaran hasil pemodelan topik dengan LDA. Analisis topik menghasilkan deskripsi topik yang dapat mewakili isi dari topik tersebut. Deskripsi topik dapat dimanfaatkan oleh pembaca atau pengguna Twitter dalam memahami segala perkembangan isu terkini.
Keyword: latent dirichlet allocation, pemodelan topik, Twitter

Judul: Topic Analysis of Twitter User About Covid-19 Vaccination in Indonesia Using Latent Dirichlet Allocation
Abstrak: Pandemi Covid-19 mendorong banyak pihak agar mampu beradaptasi dengan kondisi terkini. Salah satu program yang diluncurkan pemerintah agar dapat mengatasi penyebaran Covid-19 adalah dengan menjalankan progam vaksinasi. Agar dapat mengetahui animo masyarakat terkait program vaksinasi Covid-19 yang diluncurkan, perlu dilakukan analisis topik. Tujuan penelitian ini adalah mengetahui topik-topik terkait vaksin Covid-19 yang dibicarakan masyarakat di Twitter dan melakukan analisis sentimen pengguna Twitter terhadap vaksinasi corona. Untuk mendapatkan topik-topik pembicaraan terkait vaksin Covid-19 digunakan metode Latent Dirichlet Allocation (LDA). Metode penelitian yang dilakukan meliputi praproses data, pelabelan sentimen, penentuan jumlah, pemodelan topik, dan analisis topik. Hasil dari penelitian yang yang dilakukan adalah berupa topik-topik terkait vaksinasi Covid-19 yang sedang diperbincangkan di media Twitter di Indonesia. Tiga topik besar yang dibicarakan yaitu mengenai vaksinasi gratis oleh pemerintah Indonesia, sebab akibat mengikuti vaksinasi, dan varian vaksinasi., The Covid-19 pandemic has pushed many stakeholders to be able to adapt to the current conditions. One of the programs launched by the government to overcome the spread of Covid-19 is to run a vaccination program. To know the public's interest regarding the Covid-19 vaccination program that was launched, it is necessary to conduct a topic analysis. The purpose of this study was to find out the topics related to the Covid-19 vaccine that were discussed by the public on Twitter and to analyze the sentiments of Twitter users towards corona vaccination. To get topics of discussion related to the Covid-19 vaccine, Latent Dirichlet Allocation (LDA) method is used, while the Random Forest algorithm is used to carry out sentiment analysis. The research methods include data preprocessing, sentiment labeling, number determination, topic modeling, and topic analysis. The results of the research carried out are in the form of topics related to Covid-19 vaccination which are being discussed on Twitter media in Indonesia. The three major topics discussed were about free vaccination by the Indonesian government, the causes and effects of following vaccination, and vaccination variants.
Keyword: topic analysis, Covid-19, vaccination, LDA, Twitter

Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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