Judul: Desain Strategi Manajemen Risiko Iklim di Indonesia
Abstrak: Komitmen Indonesia dalam melawan perubahan iklim, terlihat pada keikutsertaan dalam menandatangani Perjanjian Paris yang berisikan pembatasan kenaikan suhu global tidak lebih dari 2 oC dan mengeluarkan peraturan-peraturan yang berkaitan termasuk Permen LHK No. P.7 Tahun 2018 tentang pedoman kajian kerentanan, risiko, dan dampak perubahan iklim. Penelitian ini menggunakan tinjauan literatur sistematis dalam mendapatkan informasi dampak dan risiko iklim di berbagai tempat dalam menyusun data indikator sesuai Permen tersebut, lalu menyusunnya menjadi beberapa kebijakan; serta MCA digunakan dalam menentukan skala prioritas dari kebijakan yang dibuat. Pencarian literatur menggunakan Scopus menggunakan string yang telah ditentukan dengan rentang tahun 2016-2024 untuk melihat respons terhadap Perjanjian Paris. Kebijakan yang didapatkan pada penelitian ini, yaitu asuransi iklim pada sektor pertanian, peningkatan kapasitas, peningkatan kesejahteraan masyarakat pesisir, Pemaksimalan ruang terbuka hijau, dan pemberdayaan masyarakat adat, dengan peningkatan kapasitas menjadi skala prioritas utama pada penilaian MCA. Saran untuk desain kebijakan kedepannya harus multi sektor, keberlanjutan, dan promosi., Indonesia's commitment to fighting climate change can be seen from its participation in signing the Paris Agreement which contains limits on global temperature rise to no more than 2 OC and issued related regulations including Minister of Environment and Forestry Regulation No. P.7 of 2018 concerning guidelines for assessing the vulnerability, risks and impacts of climate change. This research uses a systematic literature review to obtain information on climate impacts and risks in various places in compiling indicator data according to the Ministerial Regulation, then compiling it into several policies; and MCA is used in determining the priority scale of the policies being made. Literature search using Scopus string which has been determined with a range of 2016-2024 to see the response to the Paris Agreement. The policies obtained in this research are climate insurance in the agricultural sector, increasing capacity, improving the welfare of coastal communities, maximizing green open space, and empowering indigenous communities, with increasing capacity being the main priority scale in the MCA assessment. Suggestions for future policy design must be multi-sectoral, sustainable and promotional.
Keyword: Climate Change Impact, Climate Risk, Climate Risk Management, MCA

Judul: Evaluation of Climate Change Adaptation Related Programs in Indonesia
Abstrak: Indonesia telah menunjukkan komitmen yang kuat dalam mengatasi perubahan iklim, terutama melalui aksi adaptasi. Komitmen ini tercermin dalam ratifikasi berbagai perjanjian internasional dan pengembangan Nationally Determined Contribution (NDC). Namun, pengukuran capaian adaptasi masih menghadapi tantangan karena kurangnya kerangka kerja yang terpadu dan keterbatasan sumber daya. Penelitian ini bertujuan untuk mengevaluasi capaian aksi adaptasi perubahan iklim yang dilakukan oleh Pemerintah Indonesia, terutama dalam merespons dampak perubahan iklim pada sektor bencana. Penelitian ini menggunakan analisis risiko iklim komprehensif dan kerangka kerja Tracking Adaptation and Measuring Development (TAMD) untuk menilai manajemen risiko iklim dan implementasi aksi adaptasi. Hasil penelitian menunjukkan bahwa capaian manajemen risiko iklim masih perlu ditingkatkan, terutama dalam hal pendanaan. Program Kampung Iklim (Proklim) memiliki capaian terbaik, namun semua program menghadapi kendala pendanaan. Evaluasi juga menunjukkan keterbatasan data dan indikator untuk mengukur hasil implementasi aksi adaptasi. Hasil penelitian ini diharapkan dapat meningkatkan sinergi antara pemangku kepentingan dan memperkuat mekanisme pendanaan untuk mendukung aksi adaptasi perubahan iklim secara berkelanjutan di Indonesia., Indonesia has strongly committed to addressing climate change, particularly through adaptation actions. This commitment is reflected in the ratification of various international agreements and development the Nationally Determined Contribution (NDC). However, measuring adaptation achievements remains challenging due to the lack of an integrated framework and limited resources. This study aims to evaluate climate change adaptation actions taken by the Government of Indonesia, especially in responding to climate change impacts on the disaster sector. This research uses a comprehensive climate risk analysis and Tracking Adaptation and Measuring Development (TAMD) framework to assess climate risk management and adaptation action implementation. The results show that climate risk management achievements still need to be improved, especially in terms of funding. The Climate Village Program (Proklim) has the best achievements, but all programs face funding constraints. The evaluation also showed limited data and indicators to measure the results of adaptation action implementation. The results of this research are expected to improve synergies between stakeholders and strengthen funding mechanisms to support climate change adaptation actions in a sustainable manner in Indonesia.
Keyword: adaptasi, perubahan iklim, Manajemen Risiko Iklim, Resiliensi

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.