Judul: Analisis Perilaku Penggunaan Tas Belanja pada Ibu Rumah Tangga Tidak Bekerja Menggunakan Theory of Planned Behavior.
Abstrak: Penggunaan tas belanja merupakan salah satu perilaku ramah lingkungan yang masih perlu didorong. Penelitian ini bertujuan menganalisis pengaruh karakteristik contoh, sikap terhadap perilaku, norma subjektif, kontrol perilaku, dan niat terhadap perilaku penggunaan tas belanja berdasarkan theory of planned behavior. Penelitian ini menggunakan desain cross sectional study. Lokasi yang dipilih secara purposive adalah Desa Cijujung dan Kelurahan Panaragan. Contoh dalam penelitian ini adalah 100 ibu rumah tangga. Data dikumpulkan melalui wawancara meliputi variabel karakteristik contoh dan variabel dalam theory of planned behavior. Hasil penelitian menunjukkan bahwa sikap terhadap perilaku, norma subjektif, kontrol perilaku, dan niat termasuk dalam kategori sedang. Berdasarkan hasil uji korelasi, terdapat hubungan yang positif dan signifikan di antara variabel sikap terhadap perilaku, norma subjektif, kontrol perilaku dengan niat serta di antara variabel niat dan perilaku. Hasil uji regresi menunjukkan bahwa kontrol perilaku berpengaruh positif signifikan terhadap niat. Frekuensi penggunaan tas belanja dipengaruhi secara positif oleh niat, norma subjektif, dan kontrol perilaku serta dipengaruhi secara negatif oleh sikap terhadap perilaku.
Keyword: kontrol perilaku, niat, norma subjektif, sikap terhadap perilaku, tas belanja

Judul: Analisis niat pembelian produk melalui Online Shop pada Mahasiswa dengan pendekatan Theory Of Planned Behavior
Abstrak: Perkembangan internet memberikan dampak positif pada bidang perdagangan yang ditandai dengan lahirnya online shop. Sekarang ini, banyak online shop yang berkembang di Indonesia dengan berbagai macam bentuk seperti sebagai media perantara yang mempertemukan penjual dan pembeli di dunia maya. Tujuan penelitian ini adalah menganalisis pengaruh sikap terhadap perilaku, norma subjektif, dan kontrol perilaku yang dipersepsi terhadap niat pembelian di online shop. Penelitian ini mengggunakan design cross sectional-study dengan contoh sebanyak 100 mahasiswa Pascasarjana (S2) IPB yang tercatat aktif sebagai mahasiswa tahun ajaran 2013/2014. Teknik penarikan contoh penelitian ini adalah purposive sampling yang diambil secara proporsional dari 4 fakultas terpilih. Hasil penelitian menunjukkan bahwa karakteristik contoh yang memiliki hubungan dan pengaruh terhadap variabel Theory of Planned Behavior (TPB) adalah pendapatan, intensitas akses internet, jumlah sumber informasi, dan jumlah alasan membuka website. Sikap terhadap perilaku, norma subjektif, kontrol perilaku yang dipersepsi, dan niat pembelian saling berhubungan dan memengaruhi sesuai yang dihipotesiskan. Jadi, dapat dikatakan bahwa penelitian ini sesuai dengan Theory of Planned Behavior., the birth of online shop. Nowadays, many online shops grow in Indonesia on many forms such as online media that bring together the sellers and buyers in virtual world. The purpose of this study was to analyze the influence of attitudes toward behavior, subjective norms, and perceived control behavior toward purchase intentions through online shop. This research used cross-sectional design with 100 postgraduate students (S2) of IPB who are active as a student of 2013/2014 academic year. Sampling technique of this study was purposive sampling taken proportionally from 4 selected faculties. The result showed that the sample’s characterisric who have correlation and influence toward the Theory of Planned Behavior (TPB) variables were income, internet access intensity, mount of information resources, and mount of reasons to access the website. Attitude toward behavior, subjective norm, perceived control behavior, and purchase intention were interconnected and influenced appropriate with the hyphotesis. So, it can be said that this study was consistent with TPB
Keyword: Niat pembelian, Online shopping, Theory of Planned Behavior

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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