Judul: Diteksi antibodi anti-escherichia coli K99 dalam kolostrum induk sapi friesian holstein post vaksinasi escherichia coli polivalen dengan teknik elisa
Abstrak: Penelitian ini bertujuan untuk mendeteksi adanya antibodi terhadap Escherichia coli (E. coli) K99 di dalam kolostrum sapi Friesian Holstein (FH) yang divaksin dengan vaksin E. coli polivalen. Vaksinasi terhadap enam ekor induk sapi dilakukan sebanyak 3 kali yaitu pada 8, 6, dan 4 minggu sebelum induk diperkirakan melahirkan. Deteksi antibodi dilakukan menggunakan teknik ELISA dengan metode tidak langsung. Hasil pengamatan menunjukkan bahwa antibodi terhadap E. coli dapat dideteksi di dalam kolostrum hasil pemerahan ke-1 sampai dengan ke-9. Konsentrasi kolostrum ke-1 sampai ke-3 berkisar antara 1,054-1,129 μg/100μl. Konsentrasi antibodi tertinggi terdapat di dalam kolostrum hasil pemerahan pertama, yaitu sebesar 1,129±0.005 μg/100μl. Hasil penelitian ini dapat disimpulkan bahwa induk sapi bunting trimester akhir yang divaksin dengan vaksin E. coli polivalen mampu memproduksi antibodi spesifik terhadap E. coli K99 di dalam kolostrum dari kolostrum hasil pemerahan ke-1 sampai ke-9 dengan konsentrasi IgG total yang makin menurun pa
Keyword: Escherichia coli K99, Bovine colostrum, ELISA

Judul: Deteksi Antibodi Anti-Escherichia coli K99 Dalam Kolostrum Induk Sapi Friesian Holstein Post Vaksinasi Escherichia coli Polivalen dengan Teknik
Abstrak: MIZWAR AMANSYAH. B04051631. Detection of Anti-Escherichia coli’s antibody for Friesian Holstein’s cow colostrum Post E. coli Vaccination with ELISA Method. Under directed: ANITA ESFANDIARI dan SRI MURTINI. This experiment was conducted to detect Escherichia coli (E. coli) K99 antibody in colostrum of Friesian Holstein’s cows vaccined by E. coli K99. Six pregnant cows were vaccined three times at 8, 6, 4 weeks before parturition. The vaccine was given by intramuscular with 5 ml/ animal doses. The colostrum was collected until 5th day after parturition. Immunoglobulin Gamma (IgG) antibody in the colostrum was detected using indirect ELISA method. The result of research shown that antibody can detected from first until colostrum 9, with concentration about 1,054 – 1,129 μg/100 μl. The highest colostrum concentration there in colostrum 1 with 1,129 ± 0,005 μg/100 μl. The conclusion of this research is pregnant cows which given vaccine E. coli polivalen can produce of spesific antibody from E. coli K99 in colostrum first until colostrum nineth with IgG concentration have decrease from colostrum first until colostrum third. Key word: Escherichia coli K99, bovine colostrum, ELISA
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Judul: Thermal Characteristics and Dynamics of Tropical Cyclone over Western North Pacific Ocean: A Case Study of Typhoon Songda (Chedeng) 2011
Abstrak: The frequent occasion of Tropical Cyclone (TC) over Western North Pacific (WNP) Ocean lies from 10˚N to 26˚N and 121˚E to 170˚E. Sources of TC development come from warm ocean temperature where latent heat is released during condensation processes and subsequently concentrated in the boundary layer. Relative humidity at 850-700 hPa is responsible to produce the most intense storm. Meanwhile, weak vertical wind shear between 850-200 hPa is one of the dynamical factors that lead to the development of TC. Pressure level of 850-200 hPa is chosen to minimize the thermodynamic effect in the lower troposphere. Typhoon Songda (Chedeng in the Philippines) or TSC lasted from May 18th to 30th, 2011 and strengthened into powerful Super Typhoon stage between May 24th and 27th. Long curving track and the exhibition of all sequences of TC life cycle, TSC is then considered as an ideal case of TC. The objective of the study with case of TSC is to investigate the role of thermal characteristics and dynamics on TC intensity. The research is carried out by using Advanced Research of Weather Research and Forecasting model (WRF ARW model) version 3.3. Two domains are employed in the model. The first domain situate from 02˚N to 40˚N and 118˚E to 149˚E and cover spatial resolution () of 33.1 km. The second domain situate from 09˚N to 27˚N and 119˚E to 132˚E with of 11 km. Timestep of the model () is 120 seconds. The model is supported by the following physical parameterization, i.e., Yonsei University Scheme (YSU) in the boundary layer, Kain-Fritsch scheme (KF) in the cumulus, and WRF Single-Moment 3-class scheme in the microphysics. Initial meteorological condition prior to the formation of the cyclone is generated using WRF ARW standard-release model. Sensitivity of the model output is obtained from a selected single-parameter change scenarios, namely, 10 % to 20 % decrease and increase of wind shear initial data at 850-200 hPa, and by 10 % to 20 % decrease of relative humidity initial data at 850-700 hPa. The result showed that the negative correlations between wind shear and intensity are found in wind shear scenarios. Wind shears of 10 ms-1 which lead to strengthening of the wind intensity in the early period of Typhoon development are found in wind shear scenarios. The effect of wind shear changes on wind intensity is statistically significant in the 20 % wind shear scenarios. During mature stage, weak wind intensity occurred when wind shear increase as given in the WRF standard-release and the 20 % decreasing scenario. Different situation is found in the 20 % increasing scenario where wind shear tend to weak and lead to strong wind intensity at this stage. Meanwhile, the reduction of moisture supply at 850-700 hPa resulting from 20 % decreasing relative humidity play a major role in the weakening of wind intensity and reduces mechanical energy of the cycle process as much as 300 J kg-1.
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