Judul: Metode Pemulus dengan Regresi Tertimbang Lokal Kekar
Abstrak: Kajian ini bertujuan untuk membandingkan garis regresi yang diperoleh melalui metode OLS (Drdinrrry Least Square)dengan suatu metode kekar terhadap pengingkaran anggapananggapan formal, yaitu LOWESS (LocaLLy Weighted Smoothing Scat terpLots). Data referensi yang digunakan adalah data sekunder, hasil penelitian Puslitbang Gizi, Bogor, mengenai ."keadaan kesehatan dalam hubungannya dengan keadaan gizi ibu hamil" Sebagai peubah respons digunakan data ntropometri bayi lahir yaitu bobot dan panjang bayi lahir hidup dengan peubah prediktor lama dikandung.
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Judul: Kajian Perbandingan Metode Least Median of Squares dan Maximum Likelihood Type pada Regresi Kekar
Abstrak: Keberadaan pencilan dapat memengaruhi pendugaan parameter dalam analisis regresi yang menggunakan metode ordinary least squares (OLS). Metode regresi kekar dapat digunakan sebagai pendekatan alternatif dari OLS. Penelitian ini dilakukan untuk mengkaji perbandingan dari dua metode regresi kekar, yaitu least median of squares (LMS) dan maximum likelihood type (M) melalui data simulasi. Data simulasi diperoleh dengan membangkitkan bilangan acak berdasarkan model regresi linear berganda dua peubah penjelas. Kombinasi yang digunakan adalah jenis pencilan, proporsi pencilan, dan ukuran contoh. Berdasarkan nilai bias mutlak dan kuadrat tengah galat (KTG) diperoleh bahwa metode M lebih tepat diterapkan ketika data mengandung vertical outlier atau good leverage point, lalu diikuti oleh metode LMS. Sementara itu, metode LMS dapat menduga parameter regresi pada data yang mengandung bad leverage point. Analisis selanjutnya dilakukan pada data pertanian mengenai pengaruh pupuk organik dan konsumsi beras per kapita terhadap produksi padi sawah di 34 provinsi tahun 2017. Hasil yang diperoleh menunjukkan bahwa metode LMS menghasilkan nilai bias mutlak dan KTG terkecil., The presence of outliers can be affect to estimate the parameters of the linear regression using ordinary least squares (OLS). The robust regression methods can be used as an alternative to OLS. This study aims to compare the performance of two methods, namely least median of squares (LMS) and maximum likelihood type (M) using simulation study. The simulation data obtained by generating random number based on multiple linear regression with two explanatory variables. With combination of the sample size, outliers type, and the number of outliers. Based on the criteria: absoulut bias and mean square error (MSE), the M-estimator gave better result than the LMS-estimator when the data contains vertical outliers or good leverage point. Meanwhile, the LMS-estimator gave better result at data containing bad leverage points. The next step is analysed data application for agriculture on the affect of organic fertilizer and rice consumption per capita against the use of rice production in 34 provinces in 2017 using the best method based on data characteristic. The results from LMS-estimator produce the smallest absolut bias and MSE.
Keyword: LMS, M, OLS, Outlier, Robust Regression

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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