Judul: Faktor-faktor yang mempengaruhi pembuatan teras dan persepsi petani terhadap teras bangku pada tanah pertanian lahan kering di wilayah DAS Citanduy: studi kasus di Sub DAS Cijolang, Kabupaten Kuningan
Abstrak: Salah satu penyebab rusaknya sumberdaya tanah pertanian lahan kering di Sub DAS Cijolang Kabupaten Kuningan, adalah laju pertumbuhan penduduk yang tinggi dan cara bertani yang kurang tepat, dimana mereka terpaksa menggarap tanah-tanah marginal yang mempunyai risiko kerusakan yang tinggi. Sehingga akibatnya telah menimbulkan masalah lingkungan yang serius di wilayah tersebut.
Keyword: Terracing, persepsi petani

Judul: Evaluasi Kuakitas Disain Dan Kondisi Teras Bangku DiDaerah Aliran Sungai Citanduy  : Studi Kasus Lokasi Model Farm Purwaraj Dan Dampaknya
Abstrak: Desa Purwaraja terletak di wilayah pebukitan DAS Citanduy, di mana penduduk telah membuka lahan marjinal untuk lahan pertanian. Di lahan tersebut, salah satu tindakan konservasi yang telah dilakukan oleh petani adalah teras bangku, baik secara subsidi (Proyek Citanduy IIS maupun secara swadaya. Proyek Citanduy II itu sendiri pada pokoknya bertujuan meningkatkan pendapatan petani dan menekan laju erosi. Keefektifan teras bangku untuk menekan erosi ditentukan oleh kualitas disain dan kondisinya. tersebut masih terbatas. Data tentang kualitas Penelitian ini dirancang sebagai studi evaluasi tentang kualitas disain dan kondisi teras bangku di Purwaraja. Data lapang yang diambil adalah mengenai kondisi teras bangku dan komponennya. Penilaian kualitas disain teras bangku didasarkan pada kriteria yang disusun oleh Tim Fak. Pertanian IPB (1984). Untuk melengkapi pemabahasan, dikumpulkan data sekunder berupa persepsi petani tentang masalah erosi dan produksi padi gogo pada lahan yang bersangkutan.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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