Judul: Periode Pembungaan dan Aplikasinya dalam Taman
Abstrak: Trees is one of the vegetation that can be an attraction in landscape. Some trees show a strong character in color which is presented by flower. Nowadays, information and studies about the types of trees and flowering period is still limited. One of the studies that can be conducted to determine the time of flowering periode is phenology. This study aims to determine the flowering period of trees and the application in site design. The study was conducted for 6 months using 10 species of trees from the different types of families. This study used a grid method to count percentage of coverage flowers in the canopy and linear regression method to determine the relation between flowering periode and climatic factors. Based on the results, Samanea saman and Tabebuia caraiba showed there is a significant influence of climate factors to flowering percentage. The result of this study showed the flowering of S. saman is significantly influenced by climatic factors, such as maximum-minimum temperature difference, daylight, and frequency of rain. The increasing of the temperature difference between minimum and maximum and daylight tends to increase flowers percentage of S. Saman, whereas the increasing of percentage frequency of rain tend to decrease it. Flowers percentage of T. caraiba also significantly influenced by daylight. The increasing of daylight tend to increase it flowers percentage. The final product of this study is a recommendation of application of flowering trees in a site design.
Keyword: phenology, garden design, flowering tree, flowering period

Judul: Flowering Periods of Scented Trees on Several Levels of NPK Fertilizer and its Application in Garden
Abstrak: Pohon adalah salah satu tipe tanaman yang dapat memberikan nilai ekologis dan estetik pada suatu taman . Beberapa pohon ada yang menunjukan karakter kuat dengan aroma, warna dan bentuk yang dihadirkan dari bunga. Pohon dengan aroma bunga yang khas di Indonesia cukup banyak, tetapi masih kurangnya penelitian tentang periode pembungaan dan informasi penataan yang sesuai dengan analisis pada taman. Studi fenologi merupakan ilmu tentang periode fase-fase yang terjadi secara alami pada tumbuhan yang dipengaruhi oleh keadaan lingkungan sekitar , yang berupa faktor internal dan eksternal. Faktor eksternal yaitu cahaya, kelembaban, curah hujan, unsur hara, dan faktor internal faktor genetik dan fitohormon. Penelitian ini bertujuan untuk mengetahui masa pembungaan pada pohon beraroma pada beberapa taraf pemberian pupuk serta aplikasi penggunaannya dalam taman . Penelitian dilakukan selama 7 bulan dengan menggunakan 3 jenis pohon dari famili yang berbeda, 1) Cananga odorata (Lam.) Hook. F.&Thoms , 2) Magnolia champaca L. dan 3) Plumeria alba L. Perlakuan pupuk terbagi menjadi 3 taraf dosis NPK dengan 3 ulangan . Pemupukan dilakukan dua kali selama penelitian yaitu pada bulan November dan Februari dengan dosis 30 gram/m², 60 gram/m² dan 90 gram/m². Hasil menunjukkan pemberian pupuk 30 gram/m² pada Cananga odorata memiliki efek yang nyata pada peningkatan persentase pembungaan, pada Magnolia champaca tidak berbeda nyata oleh dosis berapapun, dan dosis pupuk Plumeria alba berbeda nyata pada persentase pembungaan pada bulan Januari yang menghasilkan persentase bunga terendah pada dosis 90 gram/m² dan tertinggi dengan dosis 60 gram/m². Hasil pengamatan pada ketiga spesies terlihat bahwa faktor iklim mempengaruhi persentase pembungaan . Cananga odorata dipengaruhi oleh kelembaban udara , Plumeria alba dipengaruhi oleh suhu rata-rata , sedangkan Magnolia champaca unsur iklim apapun tidak berpengaruh nyata . Hasil penilaian dengan kuisioner diketahui dosis pupuk berpengaruh terhadap tingkat kekuatan aroma yang dihasilkan. Hasil persentase penilaian tingkat aroma paling tinggi dari semua spesies terlihat pada dosis 90 gram/m² dan dari persentase ini menunjukkan semakin tinggi dosis semakin kuat aroma yang dikeluarkan oleh bunga. Aroma bunga yang paling disukai responden yaitu kamboja dan yang kurang disukai adalah kenanga. Hasil dari penelitian ini berupa rekomendasi pengaplikasian penataan pohon bunga aromatik dalam suatu rencana penanaman yang diaplikasikan pada salah satu taman segitiga yang berlokasi di Fakultas Kedokteran Hewan IPB., Trees is one of plant type that can provide ecological and aesthetic value in the garden, some trees show a strong character with the aroma, color and shape of flowers. There are quite a lot of trees with a distinctive floral aroma in Indonesia, but there is still a lack of research on the flowering period and information on its application according to garden analysis. The study of phenology is the science of the period of phases that occur naturally in plants which are influenced by internal and external factors. External factors including such as light, humidity, rainfall, nutrients, and internal factors including genetic factors and phytohormones. This study aimed to determine the flowering period of scented trees at several dosage of fertilizer application and its application in the gardens. The research was conducted for 7 months using 3 species of scented tree, namely 1) Cananga odorata (Lam.) Hook.
Keyword: Flowering Period, Flowering Tree, Phenology, Planting Plan

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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