Judul: Ekspresi Gen CsNitr1-L pada Padi Transgenik dan Pengaruhnya Terhadap Variasi Pemupukan Nitrogen
Abstrak: RSARI and SUSTIPRIJATNO. Nitrogen is one of macroessential nutrients needed by rice. Half of nitrogen applied to plants lost due to evaporation, denitrification, and leaching. CsNitr1-L is a gene encodes nitrite transporter in plants, which transfers nitrite from cytosol into chloroplast. This research has been done to measure expression rate from CsNitr1-L gene on F2B3 transgenic rice and to determine optimum fertilizer amount. Research started by phenotype selection using 20 mM KNO2. DNA from rice leaves amplified using PCR with 35 S primer. Plants contained CsNitr1-L gene was fertilized summed equal with 0, 100, 200, and 300 kg/ha on field, respectively. Total RNA from rice leaves was isolated and was reversetranscriptased to cDNA. CsNitr1-L gene expression was analysed using Real Time PCR with actin as reference and unfertilized nontransgenic rice as calibrator. Result shown that transgenic rice with 100, 200,and 300 kg/ha fertilization, respectively, had gene expression up to 42-50 times compared to calibrator. While transgenic rice without fertilization had gene expression up to 99 times compared to calibrator. Optimum amount of fertilizer to stimulate pinnacle gene expression was found out to be around 0-100 kg/ha. Gene expression of undetected shows nitrite transporter gene was inactive.
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Judul: Ekspresi Fenotipe Padi Transgenik Pembawa Gen CsNitr1-L terhadap Variasi Pemupukan Nitrogen
Abstrak: Ammonium is the major nitrogen source in rice. Nitrate assimilation pathway can be modified by introducing CsNitr1-L gene encoding nitrite transporter to increase the rate of nitrate and nitrite transport. The more efficienct nitrogen absorbtion can increase plant productivity. The aim of this research was to determine the phenotype expression of CsNitr1-L gene of transgenic rice on various doses of nitrogen fertilization. Transgenic rice seed was selected by nitrite solution 30 mM and PCR. Transgenic plants treated with four different concentration of urea fertilizer (0, 100, 200, and 300 kg/ha), experience were done in three replicates. Result shown that there were significant corelation (P<0.05) between dosage and rice varieties to all response variables. The most efficient nitrogen absorbtion of transgenic rice was 37.84% on doses 100 kg/ha. This doses produced growth of 80.07 cm plant height, tiller number as many as 10.71 stem, panicle number as many as 16, and grain yield of 43.72 g/grove. Non-transgenic rice generate growth and good results with a dose of 200 kg/ha.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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