Judul: Analisis Molekuler dan Evaluasi Umur Berbunga Tanaman Padi Putatif Transgenik AtCO
Abstrak: The timing of flowering is an important character which impact yield and quality in crop plant. The flowering time control is best understood in the model Arabidopsis (Arabidopsis thaliana). Recent result from rice plant research to be related with Arabidopsis thaliana reported that flowering control is manage with fotoperiodism. Insertion of flowering gene control was done on Nipponbare rice plant to hasten of flowering time with transfer CONSTANS gene. The aim of research are evaluation and analys response transgenic rice plant putative AtCO for flowering time, and testing genes existence transcriptional factor which is responsible on flowering time use PCR method. PCR analysis use spesific primer and DNA genom as template, meanwhile for expression analysis of AtCO gene on transcriptional level done by isolation total RNA. cDNA sintesis doing by reverse transcription-PCR (RT-PCR) and amplification AtCO gene product with cDNA as a template. Result of this research showed that out of 80 transgenic plants putative AtCO there are 35 positive plants containing AtCO gene. Flowering time evaluation for 35 rice plants positive AtCO showed earlier flowering time are during 63 until 67 days than their wildtype plant are 68 days. Expression analysis of AtCO gene on 5 plants showed that only one plant expressed AtCO gene that indicated by PCR product from cDNA template.
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Judul: Analisis Molekuler dan Fenotipe Galur Padi Transgenik IR64 Generasi T3 yang Mengandung Penanda Aktivasi untuk Toleransi Kekeringan
Abstrak: Activation tagging transgenic rice cv. IR64 had been developed by introducing a construct of activation tagging transposon Ac/Ds. Phenotipic character associated with drought stress tolerance of the transgenic rice lines have not been analyzed. This study aimed to evaluate T3 generation of activation tagging transgenic rice lines cv. IR64 to drought stress treatment. The methodology of study consisted of several steps including germination of samples seeds, screening the seedling with drought strees treatment, molecular analysis of the rice lines identify hpt and bar genes, and observation of agronomic character. Results of study showed that there were 34 out of 84 plants that tolerant to drought stress. A total of 23 plants of tolerant plants were detected carrying only bar gene after PCR analysis and indicated that the plants were stable mutants. Generaly, compared to the non-transgenic plants, there were changes in several agronomic parameters of transgenic rice plants cv. IR64 T3 generation. A total of nine plants increased of its height, six plants had a faster harvesting time, and 21 plants had higher weight of 100 seeds compared by non-transgenic plants.
Keyword: Bogor Agricultural University (IPB), drought stress tolerance, activation tagged, Rice (Oryza sativa L.)

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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