Judul: Stabilitas Sisipan Kandidat Gen Toleran Aluminium pada Padi Situ Bagendit Hasil Transformasi secara In-Planta
Abstrak: Situ Bagendit is one of the rice variety that sensitive to aluminum (Al) toxicity. The variety can be developed to an Al-tolerant variety because it can be planted in both dry and wet field. The effort to develop Al tolerant varieties could be done by inserting Al tolerance genes. An Al-tolerance gene, called B11 gene has been inserted to Situ Bagendit genome by in-planta technique and produced T1 generation. The research objective was to evaluate the stability of B11 gene insertion in T1 and T2 generation of rice transgenic SB-16. The evaluation was done by rice seed selection using hygromycin antibiotic with concentration 20 mg/l, molecular analysis using polymerase chain reaction (PCR) technique, and plant phenotype observation. The result of this research showed that B11 gene had been integrated in T1 plant based on molecular analysis and plant phenotype observation. The result of seed selection showed that T2 generation still segregated for B11 gene.
Keyword: Transgenic Rice., Situ Bagendit, B11 Gene, Aluminum Tolerance

Judul: Stabilitas Sisipan Kandidat Gen Toleran Aluminium (B11) pada Padi Transgenik Asal IR64 Hasil Infeksi In-Planta
Abstrak: Rice variety IR64 is categorized as a sensitive variety to Aluminum (Al) toxicity. Development of Al-tolerant rice varieties can be done by inserting Al-tolerant gene, which one of them is B11 gene that has been succesfully isolated from Al-tolerant rice genotipe, Hawara Bunar. The B11 gene has been succesfully introduced to plant rice variety IR64 through in-planta technique and produced T1 generation. The objective of this research was to evaluate the stability of B11 gene insertion on T1, T2, and T3 generation derived from IR64 transformed variety through antibiotic selection, molecular analysis, and plant phenotipic observation. Antibiotic selection was done using hygromycin antibiotic with concentration of 20 mg/l. Gene insertion analysis was done with Polymerase Chain Reaction (PCR) technique. The result showed that B11 gene had been integrated in T0, T1, and T2 generation of IR64 transgenic plant. The B11 gene integration in T0 did not cause a chimera. The antibiotic selection showed that antibiotic-tolerant and sensitive ratio of T1 and some T2 and T3 populations was 3:1. Some of the T2 and T3 generation populations showed homozigous on B11 gene. The vegetative and reproductive characters of transgenic plant were not different to the wild tipe
Keyword: transgenic rice, IR64, B11 gene, Acid soil

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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