Judul: Place-based Landscape Planning for Conservation of The Cultural Area of Baduy Dalam Tribe
Abstrak: Suku Baduy merupakan salah satu kelompok masyarakat adat sunda yang masih memegang teguh adat istiadat leluhurnya, tujuan hidupnya didedikasikan untuk memenuhi amanah leluhur dalam menjaga keseimbangan dan keharmonisan alam semesta. Perkembangan jaman dan perubahan iklim secara global mendatangkan ancaman bagi suku-suku tradisional seperti Suku Baduy ini. Oleh karena itu, penelitian ini dilakukan untuk mengidentifikasi berbagai masalah yang mengancam kelestarian kawasan budaya Suku Baduy Dalam dan memberikan solusi akhir berupa perencanaan lanskap. Perencanaan lanskap dikembangkan melalui pendekatan place-based, pendekatan berbasis unit tempat dan nilai-nilai lokal yang membentuknya. Penelitian ini dilakukan melalui tahapan persiapan, inventarisasi, analisis, sintesis, hingga menghasilkan rencana lanskap. Unit tempat sendiri dianalisis melalui analisis bioregional dengan mengelompokkan lanskap ke dalam tiga unit bioregional: unit bioregion, unit lanskap, dan unit tempat. Berdasarkan analisis bioregional, lanskap Baduy Dalam dikelompokkan menjadi 103 unit tempat yang selanjutnya pada setiap karakter tempat dilakukan evaluasi serta pemetaan ancaman dan permasalahan. Isue utama yang ditemukan pada lanskap Baduy Dalam yaitu adanya keterbatasan sumberdaya lahan pertanian (huma) yang berimplikasi pada penurunan kesuburan lahan pada satu sisi dan permasalahan laju pertumbuhan penduduk pada sisi lain. Solusi masalah tersebut yaitu melalui pembagian ruang dengan menyeimbangkan luas penggunaan lahan produktif (huma, jami, reuma). Berdasarkan solusi tersebut dihasilkan konsep perencanaan yang dikembangkan menjadi rencana ruang, rencana sirkulasi, dan rencana vegetasi sehingga terbentuk hasil akhir berupa rencana lanskap.
Keyword: Baduy Dalam, bioregion, cultural landscape, landscape planning, place based approach

Judul: Perencanaan Lanskap Konservasi Budaya Suku Baduy Luar dan Dangka dengan Pendekatan Bioregion
Abstrak: The Baduy Tribe is a community which preserves their culture, tradition, and hold true to their ancesters believe. The Baduy Tribe has suffered some external influence towards their culture, especially effecting the Outter Baduy and Dangka community which functions as buffer area for the Inner Baduy region. The general purpose of this research is to plan a cultural conservation landscape for the Outter Baduy and Dangka Tribe using the bioregion approach. Bioregion constitues the geographical area, which its delineation is not determine by administrative or political boundaries, instead its limited by the culture domain and the nature boundaries. The research consists of: preparation, preliminary study, analysis, synthesis, and planning. Analysis is done to v determine the bioregion unit, landscape unit, and place unit. After that, evaluation in matching the bioregion characteristic of Inner Baduy with bioregion characteristic of Outter Baduy and Dangka. The matching is done in order to utilise the result of the evaluation, as a concept guide in the landscape planning to be developed. The bioregional based of planning concept in this particular research has a purpose to escalate the quality of living combining nature as a resource and human activity, through sustainable refirement and optimazing the environment in the facet of ecology, social, economy, and culture. The final result of this research are arranged as landscape planning for cultural landscape conservation of The Outter Baduy and Dangka Tribe
Keyword: The Outter Baduy dan Dangka Tribe, landscape planning, cultural conservation, bioregion

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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