Judul: Konversi Lignoselulosa Tandan Kosong Kelapa Sawit menjadi Gula Pereduksi Menggunakan Enzim Xilanase dan Selulase Komersial
Abstrak: Oil palm empty fruit bunches (OPEFB) has the potential to hydrolized into reducing sugar. This research aimed to know best concentration and time of enzymatic hydrolysis using commercial xylanase and cellulase. OPEFB was milled, delignification of OPEFB, proximat analysis, chemical hydrolysis, detoxification and decolorization, and enzymatic hydrolysis by commercial xylanase and cellulase were done. Enzymatic Hydrolysis of OPEFB was used 2 different enzymes, xylanase and cellulase, which 2 different concentrations were used, 0.25% and 0.5% as well as hydrolysis time for 48 hours and 96 hours. After 48 hours hydrolysis by xylanase showed reducing sugar level 50.5 mg/L, and after 96 hours hydrolysis by cellulase showed 56.7 mg/L. Combination hydrolysis by 0.5% cellulase and netralization by HCl 1% and CH3COOH 1% showed reducing sugar level 56.7 mg/L and 21.04 mg/L respectively, larger than hydrolysis by 0.25% cellulase. Combination Hydrolysis by 0.5% xylanase and netralization by 1% HCl and 1% CH3COOH showed reducing sugar level 50.5 mg/L and 28.47 mg/L respectively, larger than hydrolysis by 0.25% xylanase.
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Judul: Optimasi Proses Hidrolisis Kimiawi dan Enzimatis Tandan Kosong Kelapa Sawit Menjadi Glukosa untuk Produksi Etanol
Abstrak: Recently, energy especially from petroleum fuel become a serious problem for the whole countries in the world, including Indonesia. Decreasing world’s petroleum stock make its price is very high. So, it is urgent to search other alternative energy sources, such as bioethanol. Oil Palm Empty Bunches (OPEB) are found abundantly as waste of CPO production. It is rich in lignocellulose content. After delignification by white rot fungi Omphalina sp, cellulose from OPEB can be degraded to glucose as the raw material for etanol production by hydrolysis process. In this research, hydrolysis were done chemically using H2SO4 and HCl and enzymatically using cellulolytic bacteria and Trichoderma isolates. Chemical hydrolysis was carried out in autoclave at temperature of 121oC and varation in acid concentration (0.1N; 0.5N; 1N; and 2N) and hydrolysis time (20; 40; 60; 120; and 240 min). The condition was then reoptimalized by increasing the temperature to 200 0C and variation hydrolisis time (5;7,5;10;12,5; dan 15 min). Enzymatic hydrolysis were done by two methods, namely simultaneuos and separated bacth methods using Trichoderma or cellulolytic bacteria and Saccharomyces cereviceae. Delignification process increased cellulose content from 47.18% to 49.09%. The optimum condition by chemical hydrolisis with H2SO4 2N was resulted in sugar content (30,86%) with ethanol production 1.82%. By enzymatic methods, higher sugar content was obtained by Trichoderma isolate with incubation time 72 hours. The sugar content was 1.46 g/L and ethanol production was 0.33%. Ethanol production from simultaneuos process by cellulolytic bacteria was the same as ethanol production from Trichoderma by separated batch process i.e as much as 0.27 %
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Judul: Psittacosis Sebagai Zoonosis Penting Yang Berasal Dari Burung
Abstrak: Psittacosis atau ornithosis adalah penyakit menular dari burung kepada manusia. Agens penyebab psittacosis termasuk ke dalam virus psittacosis-lymphogranuloma venereum group yang disebut Chlamydia psittaci. C. psittaci hanya dapat berkembang biak di dalam jaringan hidup tetapi peka terhadap antibiotik. Penyebaran penyakit ini sangat luas, terdapat hampir di seluruh dunia. Di Indonesia penyakit ini belum pernah di diagnosa, tetapi mengingat terdapat demikian banyaknya jenis burung maka diperkirakan psittacosis ini sudah ada. Kasus pada manusia pertamakali ditemukan oleh Ritter pada tahun 1879, di Eropa. Waktu itu dikenal sebagai pneumotyphus. Chlamydia psittaci tidak hanya sebagai penyebab infeksi pada burung, juga bisa menyebabkan infeksi pada mamalia diantaranya sapi, kerbau, kambing, domba, babi dan kucing. Pada kucing dikenal sebagai feline pneumonitis. Pada domba atau kambing mengakibatkan enzootic abortion. Pada burung, kalkun merupakan unggas paling rentan diikuti oleh burung psittacine dan burung lain di luar famili psittacidae. Virulensinyapun berbeda-beda. Yang berasal dari kalkun bervirulensi tinggi, sedangkan yang berasal dari burung merpati bisa bervirulensi tinggi bisa juga bervirulensi rendah. Penularan terjadi melalui kontak langsung maupun ti- dak langsung. Penularan melalui telur tidak terjadi. Umumnya penyakit ini memperlihatkan gejala klinis berupa deman, lesu, lemah, anorexia, kurus, diarrhea ber- warna kekuningan, discharge dari mata dan hidung, conjunc- tivitis. Gejala khas pada kalkun adalah istirahat dengan sternum sebagai dasarnya, karena adanya tekanan dari bagian perut yang sakit. Sedangkan pada manusia memperlihatkan adanya demam, kadang-kadang merasa dingin, lesu, sakit kepala yang hebat, sakit pada bagian belakang badan dan photophobia…dst
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