Judul: Evaluasi Respons Makan Ikan Nila Oreohcromis niloticus dengan Metode Pemberian Pakan secara Terkondisi menggunakan Stimulus Cahaya
Abstrak: Metode yang dapat diterapkan dalam pemberian pakan ialah dengan penerapan kondisi tertentu agar menghasilkan respons terkondisi agar ikan dapat terlatih untuk mendekati lokasi pemberian pakan. Penggunaan cahaya untuk melatih ikan nila agar berkumpul di dekat cahaya pada waktu pemberian pakan yang membuat ikan akan mendekati lokasi pemberian pakan sehingga proses pemberian pakan lebih efisien. Penelitian ini bertujuan mengevaluasi pertumbuhan dan respons perilaku ikan nila dengan metode pemberian pakan secara terkondisi menggunakan stimulus cahaya. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 4 perlakuan dan 3 ulangan; Kontrol (tanpa perlakuan cahaya), P1 (warna cahaya acak, sisi pemberian pakan acak, tanpa stressor), P2(warna cahaya biru, sisi pemberian pakan sesuai sisi cahaya, tanpa stressor), P3 (warna cahaya biru, sisi pemberian pakan sesuai sisi cahaya,stressor). Hasil penelitian menunjukkan metode pemberian pakan secara terkondisi menggunakan stimulus cahaya tidak berpengaruh nyata (P>0,05) terhadap pertumbuhan dan efisiensi pakan, tetapi stimulus cahaya dan waktu latihan berpengaruh nyata (P<0,05) terhadap respons ikan nila mendekati dan bertahan pada sisi cahaya., The method that can be applied in feeding is by applying certain conditions to create a conditioned response fish can be trained to approach the feeding location. The use of light to train tilapia to gather near at the time of feeding which makes the fish will approach the feeding location so the feeding process will be more efficient. This study purposed to evaluate the effectiveness of the conditioned feeding method using a light as stimulus on the growth and response of tilapia. This study used a completely randomized design with 4 treatments and 3 replications; Control(without light treatment), P1(random light color, random feeding side, without stressor), P2(blue light color, feeding side same as light side, without stressor), P3(blue light color, feeding side same as light side, with stressor). The result showed that the conditioned feeding method using light stimulus had no significant effect (P>0,05) on growth and feed efficiency, but light stimulus and training time had a significant effect (P<0,05) on fish response of tilapia to approach stay at light side.
Keyword: stimulus cahaya, respons makan ikan nila, classical conditioning, metode pemberian pakan secara terkondisi, cahaya, kondisi, nila, pertumbuhan, respons

Judul: Evaluation of Feeding Response of Carp (Cyprinus carpio) Given Various Light Stimulus as a Sign of Eating and Stressor
Abstrak: Penelitian bertujuan mengevaluasi respon makan ikan mas yang sudah dikondisikan dengan menggunakan kilatan cahaya sebagai rangsangan terkondisi untuk perlakuan pemberian makan ikan sekaligus melihat bagaimana stressor dapat memengaruhi respon makan ikan tersebut. Cahaya berwarna digunakan sebagai tanda makan untuk dapat mengevaluasi kemampuan ikan yang dalam mengasosiasikan sinyal tertentu dengan kegiatan pemberian pakan. Penggunaan metode pada penelitian ini yaitu rancangan acak lengkap dengan melakukan 4 perlakuan dan 3 ulangan, di antaranya, P1 (warna dan sisi cahaya acak, tanpa stressor), P2(warna cahaya hijau, tanpa stressor), P3 (warna cahaya hijau, stressor) dan, Kontrol (tanpa stimulus cahaya). Hasil penelitian menunjukkan bahwa metode pemberian pakan menggunakan stimulus cahaya berwarna sebagai penanda makan berpengaruh terhadap respon ikan mas yang mampu beradaptasi atau belajar terhadap stimulus-stimulus yang diberikan berdasarkan waktu mendekati pada sumber cahaya yang diberikan, yakni rata-rata ikan pada awal pemberian stimulus membutuhkan waktu rata rata lebih dari 90 detik dan pada akhir pemberian stimulus meningkat menjadi 20–40 detik. Perlakuan cahaya maupun stressor tidak mempengaruhi pertumbuhan ikan pada pemeliharaan selama 28 hari., The aim of this study was to evaluate the conditioned feeding response of carp fish using a flash of light as the conditioned stimulus for the fish feeding treatment and to see how stressors could affect the feeding response of the fish. Colored light is used as a feeding signal to be able to evaluate the ability of fish to associate certain signals with feeding activities. The method used in this study was a completely randomized design with 4 treatments and 3 repetitions, including, P1 (color and random light side, without stressor), P2 (green light color, without stressor), P3 (green light color, stressor) and, Control (without light stimulus). The results showed that the feeding method using a colored light stimulus as a feeding marker had an effect on the response of carp fish that were able to adapt or learn to the given stimuli based on the time it took to approach the given light source, i.e. the average fish at the beginning of giving the stimulus needed time average more than 90 seconds and at the end of the stimulus increased to 20-40 seconds. Light and stressor treatment did not affect fish growth in maintenance for 28 days.
Keyword: Carp, Light, Response

Judul: Isolasi Konstruksi dan Rekombinasi Gen Pengkode Reverse Transcriptase asal Simian Retrovirus-2 (SRV-2)
Abstrak: The availability of reverse transcriptase enzym as a valuable component for Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique has been providing by foreign companies. Therefore, it needs more cost and time to provide it. This study is an early step to produce in house reverse transcriptase rekombinant enzym from SRV-2. The purpose of this study was isolated, constructed, and rekombined SRV-2 reverse transcriptase gene to entry clone and destination vector. Isolation was done by PCR amplification technique using three pairs of primers, which are RT I (3282-3683), RTII (3261-3900), and RTIII (3169-4133). Construction and recombination in this study used Gateway method from Invitrogen. SRV-2 reverse transcriptase gene was isolated in this study with A-549 cell inoculated SRV-2 as source of virus. This gene amplify by PCR technique and resulted specific band for RTI 401 bp, RTII 639 bp, and RTIII 964 bp. Analysis of pENTR/SD/TOPO and pDEST17 indicated SRV-2 reverse transcriptase gene code has already inserted to this plasmid. This result indicated SRV-2 reverse transcriptase gene have isolated, constructed, and recombined to entry clone and destination vector.
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