Judul: Dampak Keberadaan TPST Bantar Gebang terhadap Harga Rumah dan Upaya dalam Pengelolaan Sampah Berkelanjutan (Studi Kasus: Kelurahan Ciketing Udik, Kecamatan Bantar Gebang, Kota Bekasi)
Abstrak: Tempat Pengelolaan Sampah Terpadu (TPST) Bantar Gebang saat ini telah mengalami peningkatan volume sampah yang menimbulkan dampak negatif berupa penurunan kualitas lingkungan yang berdampak kepada harga rumah. Penelitian ini bertujuan untuk: (1) menganalisis persepsi masyarakat, (2) menganalisis pengaruh faktor penurunan kualitas lingkungan terhadap harga rumah, (3) menganalisis stakeholder dan upaya pemerintah dalam pengelolaan sampah yang berkelanjutan. Skala likert untuk menganalisis persepsi masyarakat, Hedonic Price Method (HPM) dan analisis linear berganda untuk mengetahui faktor-faktor yang mempengaruhi harga rumah, metode analisis stakeholder untuk melihat pengaruh dan kepentingan stakeholder, dan analisis gap untuk melihat upaya keberlanjutan dari pemerintah. Hasil analisis persepsi menunjukkan TPST Bantar Gebang menurunkan kualitas lingkungan serta memberikan dampak negatif dari sisi ekonomi, sosial, dan lingkungan. Penurunan kualitas air dan udara berpengaruh nyata terhadap harga rumah. Terdapat enam stakeholder pengelolaan sampah di TPST Bantar Gebang. Pemerintah daerah telah berupaya dalam pengelolaan sampah melalui beberapa program. Namun, dalam pelaksanaan masih belum optimal terlihat masih adanya gap antara target dan realisasi capaian tiap program., The Bantar Gebang Integrated Waste Management Site has currently experienced an increase in the volume of waste which has a negative impact in the form of a decrease in environmental quality which has an impact on house prices. This research aims to: (1) analyze public perception, (2) analyze the influence of environmental quality degradation factors on house prices, (3) analyze stakeholders and government efforts in sustainable waste management. Likert scale to determine public perception, Hedonic Price Method and multiple linear analysis to determine the factors that affect house prices, stakeholder analysis method to see the influence and interests of stakeholders, and gap analysis to see the government's sustainability efforts. The results showed that the Bantar Gebang can reduce environmental quality and have a negative impact on the economic, social, and environmental aspects. Decreasing water and air quality has a significant effect on house prices. There are six stakeholders in waste management at the Bantar Gebang. The local government has made efforts in waste management through several programs. However, the implementation is still not optimal, it can be seen that there is still a gap between the target and the realization of the achievements of each program.
Keyword: Gap analysis, Stakeholder analysis, Hedonic Price Method, Water quality, Air quality

Judul: Public Perception Analysis and Willingness To Pay (WTP) Estimation on TPS 3R as Alternative of Waste Management System (Case Study: Kelurahan Pondok Karya, Kecamatan Pondok Aren, South Tangerang City)
Abstrak: Keterbatasan lahan Tempat Pemrosesan Akhir (TPA) Cipeucang untuk menampung timbulan sampah akan berimplikasi pada timbulnya Tempat Penampungan Sementara (TPS) secara liar di Kota Tangerang Selatan. TPS liar menggunakan sistem konvensional yang akan menimbulkan dampak negatif pada lingkungan pemukiman, salah satunya di Kelurahan Pondok Karya. Paradigma sistem konvensional harus ditinggalkan dan diperbaharui dengan pola pendekatan berbasis masyarakat seperti TPS 3R (reduce, reuse, recycle). Tujuan penelitian ini adalah: (1) mengidentifikasi upaya pemerintah terhadap rencana penyelenggaraan TPS 3R, (2) mengidentifikasi persepsi masyarakat terhadap pengelolaan sampah saat ini dan tingkat partisipasi terhadap rencana penyelenggaraan TPS 3R, (3) mengestimasi nilai Willingness to Pay (WTP) masyarakat, dan (4) menganalisis faktor-faktor yang mempengaruhi besarnya nilai WTP masyarakat. Penelitian ini menggunakan metode analis deskriptif, analisis skala likert, Contingent Valuation Methode (CVM), dan analisis regresi logistik ordinal. Hasil penelitian menunjukan adanya upaya pemerintah berupa peningkatan kegiatan sosialisasi serta pemberian bantuan dana pada pembangunan TPS 3R. Masyarakat sangat setuju bahwa pengelolaan sampah saat ini tidak layak baik dari aspek sosial, lingkungan, maupun ekonomi. Tingkat partisipasi masyarakat sangat tinggi untuk mendapat pengelolaan sampah yang lebih baik. Rataan nilai WTP sebesar Rp 40.100/KK/bulan akan digunakan untuk pembiayaan operasional TPS 3R. Faktor yang mempengaruhi besaran nilai WTP adalah lama tinggal, tingkat pendapatan, status tempat tinggal, dan pendidikan., The problem of limited capacity of Cipeucang landfill (TPA) to accommodate the urban waste cause the emergence of illegal temporary landfill (TPS) in South Tangerang. The illegal TPS is using the conventional waste management system which creates negative effects for the surrounding residential areas, including Kelurahan Pondok Karya. The conventional waste management system must be replaced with the new community-based approach , such as TPS 3R (reduce, reuse, recycle). The aims of this study are; (1) identifying the government plan to execute TPS 3R; (2) identifying people’s perception regarding to the current waste management and people’s participation level to TPS 3R execution plan; (3) estimating people’s Willingness to pay, and; (4) analyzing the factors that influence people’s WTP. This study used a descriptive analytical method, likert scale, Contingent Valuation Method (CVM), and ordinal logistic regression. The result of this study shows that government has conducted socialization on TPS 3R and provide budget for TPS 3R establishements. People strongly agree that the current waste management is not feasible in social, environmental and economic aspects. People's level of participation to get a better waste management was high. Mean of WTP value was Rp 40.100,00/household/month and would be used to finance the TPS 3R operational costs. Factors that influenced the WTP are period of stay, level of income, residence status, and level of education.
Keyword: biaya operasional, persepsi masyarakat, regresi logistik ordinal, TPS liar

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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