Judul: Perbandingan Pola Pita Amplifikasi DNA Daun, Bunga, dan Buah Kelapa Sawit Normal dan Abnormal.
Abstrak: Kelapa sawit dimanfaatkan sebagai bahan baku industri minyak goreng, makanan, kosmetik, sabun, lilin, logam, serta biodiesel. Permintaan Crude Palm Oil (CPO) yang semakin meningkat kurang bisa dipenuhi sehingga perbanyakan bibit unggul kelapa sawit dilakukan dengan kultur jaringan. Namun teknik ini menghasilkan tanaman kelapa sawit yang abnormal, sehingga diperlukan penanda molekuler yang mampu mendeteksi abnormalitas secara dini. Penelitian bertujuan mengetahui perbedaan genotip daun, bunga, dan buah dari tanaman kelapa sawit normal dan abnormal, berdasarkan perbedaan pola pita amplifikasi DNA sampel terhadap primer acak menggunakan teknik amplifikasi DNA. Hasil penelitian menunjukkan perbedaan pita amplifikasi yang muncul pada sampel DNA bunga abnormal dengan primer OPA 8, DNA buah normal dengan primer OPB 2, dan DNA buah abnormal (primer OPA 18, OPA 20, OPB 2, dan OPB 9). Fragmen DNA yang berbeda kemudian diisolasi dan disekuensing. Sekuen basa yang berhasil dibaca hanya DNA buah abnormal dengan primer OPB 9. Analisis hasil sekuen dilakukan dengan program Basic Local Alignment and Search Tool for Nucleotida (BLAST) namun tidak menunjukkan homologi yang tinggi dengan gen dalam bank gen. Hasil BLAST juga tidak menghasilkan urutan basa yang ada pada suatu gen penyandi tertentu dalam tanaman kelapa sawit.
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Judul: Analisis genotipe normal dan abnormal pada klon kelapa sawit (Elaeis guineensis Jacq.) dengan RAPD
Abstrak: The formation of flower and fruit abnormalities in oil palm still unclear. The aim of this study is to analyze the genetic similarities, grouping among normal and abnormal genotypes, and to obtained a specific DNA band for abnormalities by RAPD analysis. Plant materials have been used i.e. MK152, Mk203, MK209, and MK212 (normaVabnormal and male flowers), while clones MK104 and MK176 (normal and abnormal). Amplification of DNA samples have been done with 15 random primers. Genetic similarities and phenogram were analyzed with NTSYS-pc.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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