Judul: Efek anabolik dan androgenik ekstrak heksana buah okra(Abelmoschusesculentus L.Moench)
Abstrak: The analyse of genetic variety was done through 14 samples of timor deer (Cervus timorensis) from South East Sulawesi and Nusa Tenggara Timur by using PCR (Polymerase Chain Reaction) tehnique. Observation method were (1) Total DNA isolation, (2) Quality of DNA evaluation, (3) Amplification of genome Mitochondrial DNA D-Loop region, (4) Cutting by restriction enzyme, (5) Analyse PCR result and its cutting and (6) Data analyse. Amplification D-Loop region of deer mitochondrial DNA was done by a pair of primers. CTDF primer (5'-TAA TAT ACT GGT CTT GTA AAC C-3') stick to threonin tRNA and CTDR primer (5'- GGC TGG CAC GAG ATT TAC CAA CCC-3') stick to 12SrRNA region. Amplification through all deer samples gave a fragment, which is sized 1340 bp. Base on reference sequence data, the fragment that's got, sized 1437 bp. There are 97 bp diversities, these were caused by sequence reference using RNA, RNA, tRNA Phe and a half of 12SrRNA came from another species, that was Muntiacus reevesi (Gene-Bank, access code NC_004069) and it's not from timor deer because the fragment basic data wasn't available. Further amplification result was cut by six kinds of restriction enzyme, which is cutting enzyme four bases HaelII (GG-CC), HapII (C/CGG), Alul (AGCT) and Rsal (GT-AC) and cutting enzyme six bases BamHI (G-GATCC) and EcoRI (GAATTC). The samples gave monomorphic cutting result, we can also say, it's not detected a variation to D-Loop region of timor
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Judul: Keragaman fragmen DNA mitokondria daeha D-loop rusa timor cervus timorensis asal Sulawesi Tenggara, dan Nusa Tenggara Timur
Abstrak: Analisis keragaman genetik dilakukan pada 14 sampel rusa timor (Cervus timorensis) yang berasal dari Sulawesi Tenggara, dan Nusa Tenggara Timur dengan menggunakan teknik PCR (Polymerase Chain Reaction). Metode penelitian meliputi: (1) Isolasi DNA total, (2) Evaluasi kualitas DNA, (3) Amplifikasi daerah D-Loop genom DNA Mitokondria, (4) Pemotongan dengan enzim restriksi, (5) Analisis hasil PCR dan pemotongannya, dan (6) Analisis data. Amplifikasi daerah D-loop DNA mitokondria rusa dilakukan dengan menggunakan sepasang primer, yaitu primer CTDF (5'-TAA TAT ACT GGT CTT GTA AAC C-3') yang menempel pada tRNA threonin dan primer CTDR (5'-GGC TGG CAC GAG ATT TAC CAA CCC-3') yang menempel pada daerah 12S rRNA. Amplifikasi pada semua sampel rusa menghasilkan fragmen berukuran 1340 bp. Berdasarkan data sekuen acuan, fragmen yang didapat berukuran 1437 bp. Terjadi perbedaan sebanyak 97 bp yang disebabkan karena sekuen acuan menggunakan tRNA Thr, tRNA Pro, tRNA dan sebagian 12SrRNA berasal dari spesies lain, yaitu Muntiacus reevesi (Gene-Bank, kode akses NC_004069) dan bukan dari rusa timor, karena data dasar fragmen tersebut tidak tersedia. Hasil amplifikasi selanjutnya dipotong dengan menggunakan enam macam enzim restriksi, yakni enzim pemotong empat basa, yaitu: HaelII (GG CC), Hapll (C/CGG), Alul (AG-CT), dan Rsal (GT-AC), dan enzim pemotong enam basa BamHI (G-GATCC) dan EcoRI (GAATTC). Semua sampel memberikan hasil pemotongan yang seragam (monomorfik), dengan kata lain tidak terdeteksi adanya variasi pada daerah D-Loop rusa timor.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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