Judul: The Effect of Cytokinin and Auxin Treatment in the Multiplication Through in vitro Phase to Acclimatization of Pineapple (Ananas comosus (L) Merr.) cv. Smooth Cayenne.
Abstrak: The mass propagation of pineapple are hampered by low multiplication rate in natural way, therefore in vitro propagation is an alternative to overcome mass production. To support mass propagation then we need the best acclimatization way to support multiplication phase to achieved the highest mass propagation rate. The objective of this research was to elucidate the effect of Cytokinin (BA) and Auxin (NAA) that had been applied during multiplication of sub culture I and sub culture II through in vitro phase to acclimatization of pineapple (Ananas comosus L. Merr) cv. Smooth Cayenne. This research was conducted in greenhouse laboratory at Center for Tropical Fruits Studies, IPB Baranangsiang, Bogor, since July until November 2006. This research used 20 combination of treatment from two factors for each subculture 1 and subculture 2. Each subculture conducted in six groups to accomodate difference planlet’s acclimatization readiness. The first factor was 0,00μM, 4,40μM, 8,80μM, 13,32μM, and 17,76μM of BA, and the second factor was 0,00μM, 0,50μM, 1,00μM, 2,00μM of NAA. Number of leaves and plant’s height were examined every week until 13th week after acclimatization. Examination data were statistically analyzed using Complete Randomized Block Design and the means were compared with Duncan’s Multiple Range Test (DMRT) procedure at 5% level of significance. The result showed that treatment of 17,76μM BA and 2,00μM NAA, and its interactions have the highest score for subculture 1. The result of 2,00μM NAA showed the highest performance for subculture 2. Overall performance showed that subculture 1 has better growth rate than subculture 2.
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Judul: Pengaruh sitokinin (TDZ) dan auksin (IAA dan NAA) terhadap multiplikasi nenas, Ananas comosus L. Merr. cv. Queen dalam perbanyakan kultur jaringan
Abstrak: The objective of this research was to study the effect of Cytokinin (TDZ) and Auxin (IAA and NAA) as growth regulators in shooting multiplication capacity of shoot base pineapple explant. This research was conducted in tissue culture laboratory at Tropical Fruits Study Center. IPB. Baranangsiang. Bogor. since January until September 2004. Planting material of 5-10 mm base shoot of third subculture Queen pincapple's planlet were cultured in shooting induction medium consisted of MS suplemented with Auxin (IAA and NAA) and TDZ in various concentrations. The experiment was set up in factorial design with two factors, Auxin (5 levels: 0 mg/1. 0.1 mg/l IAA, 0.01 mg/l IAA, 0.1 mg/I NAA and 0.01 mg/l NAA) and TDZ (6 levels: 1x10 mg/l. 1x10 mg/l, 1x10 mg/l, 1x10 mg/l, 1x10 mg/l and 1x10 mg/l). There were 30 combinations treatment. Each treatment was consist of 4 replicates, containing 6 explants per replicate, so that found 720 unit. After 20 weeks in shooting induction medium, explants were transferred to MSO medium for shooting multiplication. Each treatment was consist of 12 replicates. In shooting induction phase, the percentage and the the number of shoot. root. nodule and chap nodule were examined. After subculture to MS free medium, the number of axillar shoot, adventitious shoot and chap nodule were examined every weeks. The weight of nodule were examined in the first and last week of observation. Meanwhile. the number of shoot per gram nodule weight were also examined in the last week of observation. The data were statistically analyzed using completely randomized design and the means were compared with Duncan's Multiple Range Test (DMRT) procedure at 5% level of significance. In the first week of the culture shoot induction medium. explants have shown its growing which marked with crosscut leaves elongation and new leaves formation. In almost treatments, shoot started to be formed at 2 weeks after culture (MST) and rooted at 3 MST. except for the treatments of TDZ at concentration 1x10 mg/l. which is combined with various Auxin concentrations. Explants grown in media supplemented with 1x10 mg/l. 1x105 mg/l, 1x10 mg/l. 1x10 mg/l TDZ in different concentrations of Auxin. initially responded by shoot and root formation with no nodules. There were little number of shoots (with amount of mean less than two) that formed in these culture medium by the end of perception week in both phases, shoot induction phase and shoot multiplication phase. Although the shoots cultured in media supplemented with low concentrations of TDZ (below 1x102 mg/l) were fewer in number. but the shoots were longer and have better quality than those formed in media supplemented with high level concentrations of TDZ (above 1x10 mg/) in the same perception week. Shoots became capable of rooting at combinations of 1x10- 1x10 mg/l TDZ with different concentrations of Auxin, but 1x10 mg/l without Auxin gave rise to the best rooting...dst
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Judul: Formulasi Hamiltonian untuk Menggambarkan Gerak Gelombang Soliter Dimensi Tiga di Permukaan Laut
Abstrak: Gelombang permukaan adalah gelombang yang terjadi perbedaan rapat massa antara air dan udara. Salah satu gelombang permukaan yang ditinjau adalah gelombang soliter. Formulasi gelombang soliter dilakukan dengan menggunakan formulasi Hamiltonian.
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