Judul: Pengaruh Tekanan Ekonomi dan Interaksi Orangtua-Remaja Terhadap Motivasi Menikah Dini
Abstrak: Anak memiliki perlindungan di dalam hukum salah satunya terbebas dari pernikahan anak. Pernikahan yang terjadi pada usia anak memberikan dampak negatif terhadap tumbuh kembang anak dan keluarga yang dibentuk. Pernikahan anak tidak hanya disebabkan oleh faktor eksternal tetapi faktor internal remaja, seperti keinginan remaja untuk menikah dini. Penelitian ini bertujuan untuk mengetahui pengaruh tekanan ekonomi dan interaksi orang tua-remaja terhadap motivasi remaja untuk menikah dini. Penelitian ini menggunakan desain penelitian cross sectional dengan teknik voluntary sampling. Jumlah sampel penelitian sebanyak 165 remaja di Jabodetabek dan tinggal bersama kedua orang tua. Hasil analisis korelasi menunjukan bahwa tekanan ekonomi subjektif memiliki hubungan positif signifikan dengan motivasi menikah dini dan tingkat pendidikan ibu memiliki hubungan negatif signifikan dengan motivasi menikah dini. Hasil analisis pengaruh menunjukan tekanan ekonomi subjektif dan interaksi orang tua-remaja pada dimensi kekerasan dan kontrol psikologi berpengaruh positif signifikan terhadap motivasi menikah dini.
Keyword: child marriage, early marriage motivation, economic pressure, parent-adolescent interaction

Judul: Pengaruh Tekanan Ekonomi dan Penyesuaian Perkawinan terhadap Kepuasan Perkawinan Keluarga yang Menikah Muda
Abstrak: Pernikahan adalah ikatan lahir batin antara pria dan wanita sebagai suami istri dengan tujuan membentuk keluarga yang bahagia atau kekal berdasarkan Ketuhanan yang Maha Esa. Pernikahan muda adalah pernikahan yang dilakukan pasangan yang menikah di bawah usia 20 tahun. Penelitian ini bertujuan untuk mengetahui pengaruh tekanan ekonomi dan penyesuaian perkawinan terhadap kepuasan perkawinan keluarga yang menikah muda. Penelitian ini melibatkan 60 keluarga yang dipilih secara snowball dengan istri sebagai contoh. Analisis data menggunakan uji deskriptif, uji korelasi, dan uji regresi berganda. Hasil penelitian menunjukkan adanya hubungan negatif signifikan antara tekanan ekonomi obyektif dengan kepuasan perkawinan. Hasil penelitian juga menunjukkan adanya pengaruh positif signifikan antara besar keluarga, tekanan ekonomi subyektif, dan penyesuaian perkawinan terhadap kepuasan perkawinan serta pengaruh negatif signifikan antara tekanan ekonomi obyektif dengan kepuasan perkawinan.
Keyword: kepuasan perkawinan, menikah muda, penyesuaian perkawinan, tekanan ekonomi

Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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