Judul: Analisa kebutuhan ruang parkir kendaraan pada pusat pertokoan kota Pekanbaru
Abstrak: Pesatnya pertumbuhan fisik kota-kota dalam beberapa dasawarsa terakhir telah memunculkan konsekuensi logis berupa meningkatnya permintaan terhadap ruang lahan untuk pembangunan pusat-pusat kegiatan bisnis, komersil, perdagangan, industri, perumahan dan lain-lain. Hingga batas tertentu pembangunan pusat kegiatan berskala besar seperti plaza, mal, supermarket, swalayan dan lain-lain telah memenuhi kebutuhan sarana ruang parkir untuk menghindari kemacetan lalu lintas yang dimungkinkan terjadi pada ruas jalan di sekitarnya.
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Judul: Preferensi masyarakat terhadap ketersediaan taman kota di Kota Pekanbaru – Propinsi Riau
Abstrak: Urban park is one type of public open space in urban areas that people use to interact with the environment and other people. At present, many parks are available at urban area but rarely used or visited by the urban community because of those parks are not suitable to people preferences. This study aims to determine and analyze community preferences on the availability of urban park in Pekanbaru City. The method used in this study were qualitative and quantitative methods of analysis, which consist of content analysis and Multiple Correspondency Analysis (MCA). The study showed that the current system of urban park system in Pekanbaru City are the distribution pattern of the park where the park has not been evenly spread across the center of city, the availability of the park is still low (0.17 m2/jiwa of 0.3 m2/person it should be) and 93.8% of the whole park is a large garden (> 1500 m2), the shape and design of the park tend to be equal to the characteristic feel of Malays in the ornaments, the dominant function of the park that is a function of architecture as part of the aesthetic aspects of the city, and facilities predominantly recreational park (children playground, lawn, sports facilities (reflection track, volleyball court, basketball court and the tenant of the park is dominated by the city government's. Analysis of legal aspects related park system shown that the existing regulatory system already supports existing landscaping, but has not spelled out in technical detail and the lack of community participation in planning and formulation of regulations. Community preferences to park variables are park location close to settlements (<1,000 m), large park size (> 1500 m2), the function as a place of recreation and sports, and facilities within the park area. Recommendations resulting from this study that urban park availability based on community preference in Pekanbaru City are distribution within the city evently, short distance from home or center of daily activities, active type of recreation, quite large park size, organic design, save and comfort park. The recommendation of the availability model of a city park are expected to contribute to the Government of Pekanbaru City in determining the policies and actions in the management of urban parks in the future.
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Judul: Study of Tupaia javanica Hepatocytes Culture as In Vitro Replication Media of Nonhuman Primate HBV
Abstrak: Virus hepatitis B (VHB) adalah virus DNA yang menginfeksi organ hati sebagai target utama. Infeksi ini menyebabkan hepatitis yang dapat bersifat kronis dan berlanjut menjadi sirosis, kanker hati bahkan kematian. Pengembangan model in vivo dan in vitro berkaitan dengan hepatitis B menjadi perhatian khusus bagi para peneliti. Kultur hepatosit banyak digunakan untuk mempelajari berbagai ha] terkait hepatitis B baik dari aspek virus, penyakit, pencegahan, dan pengobatan. Pengembangan hepatosit asal hewan kecil sepe1ii Tupaia sp. untuk model in vitro merupakan salah satu altematif dalam penyediaan kultur hepatosit. Tujuan penelitian ini adalah mengembangkan kultur primer dan lestari hepatosit T. javanica yang mampu mendukung replikasi VHB orangutan (OuHBV) dan VHB owa jawa (GiHBV), dan dapat menjawab kebutuhan akan model in vitro kultur hepatosit. Isolasi hepatosit T. javanica dilakukan dengan prosedur sebagai berikut: organ hati yang telah dicacah dalam cawan petri dengan larutan perfusi, diinkubasi pada 37 °C. Suspensi disentrifugasi 300-500 g sehingga didapatkan pelet sel hati. Pelet yang sudah dicuci kemudian diresuspensi dengan Hepatocyte Basal Media untuk menumbuhkan hepatosit dan selanjutnya hepatosit akan ditransformasi menggunakan perangkat Lenti-SV 40. Sodium taurocholate cotransporting polypeptide (NTCP) yang diindikasikan sebagai reseptor hepatosit untuk melekatnya VHB, nampak pada hepatosit baik pada kultur primer maupun kultur yang ditransformasi. Reseptor hepatosit spesifik ini terlihat sebagai pendaran hijau dengan uji imunofluoresensi. Kultur hepatosit primer T. javanica berhasil dikembangkan selama 14 hari untuk mencapai kepadatan 80%, dan inokulasi GiHBV dan OuHBV ke dalam kultur pada hari ke-15 menunjukkan adanya replikasi virus hingga hari ke-8 (berdasarkan hasil PCR). Kuantifikasi virus dengan real-time PCR memperlihatkan replikasi tertinggi pada hari kedua setelah inokulasi. Penjajaran sebagian protein permukaan setelah virus dinokulasikan memperlihatkan kesamaan asam amino dengan virus wild type sebesar 99.29% dan 95.71 % pada GiHBV dan OuHBV., Hepatitis B virus (HBV) is a DNA virus which infects liver as primary target organ. This i11fection causes hepatitis which could become chronic and progress to cirrhosis, liver cancer and even death. Development of in vitro and in vivo model related to hepatitis B is a concern for researchers. Hepatocyte cultures widely used to study a variety of aspects of hepatitis B such as the virus, disease, prevention and treatment. Development qf hepatocyte from small animal e.g. Tupaia sp. for the in vitro model is an alternative of hepatocyte cultures. The purpose of the study is to develop primary and immortal T. javanica hepatocytes cultures capable to replicate orangutan HBV (OuHBV) and javan gibbon HBV (GiHBV), and to answer the need for in vitro model ofhepatocytes. Isolation of hepatocytes from T. javanica was done as followed: mincedliver were transported into petri dish containing perfusion buffer solution to be incubated at 37 °C. The suspension was centrifuged at 300-500 g to obtain liver cell pellets. The washed-pellet was resuspended in Hepatocyte Basal Media to let specific hepatocytes grow and later be transformed by Lenti-SV40 kit. Sodium taurocholate cotransporting polypeptide (NTCP) has been ident(fied as hepatocyte receptor for HBV attachment, and was seen on hepatocytes from both primary and transformed cultures. This hepatocytes specific receptor was shown as green luminenscent by immunofluorescence assay. Primary T. javanica hepatocytes cultures was successfully maintained for
Keyword: Tupaia javanica, in vitro model, hepatocytes culture, hepatitis B virus, Liver cancer, Picornaviridae, Hepadanaviridae, Flaviviridae, Caliciviridae