Judul: Analisis Komunitas Bakteri Fruktosfer Kelapa Sawit Menggunakan Teknik Terminal Restriction Fragment Length Polymorphism (T-RFLP)
Abstrak: The knowledge about bacterial community could provide basic information for the development of further research. One method that could be used to analize the diversity of microbial community is Terminal Restriction Fragment Length Polymorphism (T-RFLP). The objective of this research is to analyze the existing bacterial diversity on oil palm fructosphere. The 16S rRNA genes were amplified by PCR using forward primer (27F) and reverse primer (926R) which have been labeled. The PCR product were then digested with four different enzymes, namely BstUI, MspI, DdeI, and Sau96I. The elecroforegram showed that these enzymes produce different patterns due to the unique restriction sites of each enzymes. In general, BstUI and MspI enzymes produce more Terminal Restriction Fragment (TRF) than two other enzymes. From T-RFLP analysis we could identify some bacterial genus. Bacteria that were predicted to be present in oil palm fructosphere were gram-positive and gram-negative bacteria, Proteobacteria, Actinobacteria, soil bacteria, marine bacteria, and bacteria that could not be cultured.
Keyword: 

Judul: Penapisan Bakteri Lipolitik asal Fruktosfer Kelapa Sawit
Abstrak: Lipase (EC 3.1.1.3, glycerol ester hidrolase) is defined as carboxylesterase that catalyzes the hydrolysis of long chain asilgliserol. Lipase produced by lipolytic bacteria that grow well in substrates containing oils such as oil palm fruit. The purpose of this study was to screen lipolytic bacteria from oil palm fructosphere and to characterize optimal pH and temperature of lipase enzyme from selected isolates. Seven isolates of lipolytic bacteria with different morphology were qualitatively screened from oil palm fructosphere using Rhodamin B media. Bacteria were isolated from the exocarp and mesocarp of ripe oil palm fruits. Lipolytic activity each isolates were measured and selected based on their activity. Three isolates possessed the best activity with result as follows EP2.22 (0.912 U/mg), F2 (0.792 U/mg), and F6 (0.650 U/mg). These isolates showed high activity at various range of pH and temperature. Isolate EP2.22 showed optimum pH at 7.5 and optimum temperature at either 40 oC or 70 oC. Isolate F2 showed optimum pH 8.5 and optimum temperature 70 oC. Isolate F6 showed optimum pH 8.5 and optimum temperature at 30 oC and 70 oC. Two peaks of high activity due to the use of crude enzyme extract. 16S rRNA gene sequencing indicated that isolates EP2.22, F2, and F6 closely related to Staphylococcus klosii Enterobacter sp., and Buttiauxella izardii.
Keyword: 

Judul: Manajemen Pemeliharaan Kuda (Equus caballus) Untuk Upacara Kenegaraan Dan Sarana Kesenjataan Di Detasemen Kavaleri Berkuda (DenKavkud) TNI-AD Parongpong
Abstrak: Cavalry Horse Detachement (Denkavkud) Indonesian National Army-Cavalary Parongpong is one of the Indonesian military units that utilize the horse and train it as a means of purpose of formal state ceremony and armed forces. Skills possessed by the horse cavalry was the result of care management and specialized training provided by the trainer. Aspects of care management are breeding, feeding, and management. Horses that are kept in the Denkavkud, Parongpong are stallions, mares, foals, and military horses. Total populations of horses at Denkavkud are 183 horses. Besides commited horse training (remonte), Denkavkud also did breeding program to produce horses that can be trained and used as a military horse. There are some issues contained in Denkavkud, such as some of the nutrients needed by the horses not being met from feed given. One of these is crude protein and lysine. Lactation mares was crude protein and lysine deficiency. The number of death in 2010 is higher than previous years. Based on the number of horses that died from January until June 2010 is 11 horses. The average mortality was caused by colic. Colic is caused by excessive eating, excessive drinking during hot, moldy food, and even by investment roundworms. Animal welfare in Denkavkud can be enhanced by improvement of feed given and improving the health of livestock through improved sanitation.
Keyword: