Judul: Populasi dan keragaman fauna tanah pada tipe penggunaan lahan di desa situgede kecamatan Bogor Barat
Abstrak: Indonesia merupakan negara tropis yang kaya akan keanekaragaman flora dan fauna, karena didukung oleh suhu dan intensitas matahari yang cukup, kelembaban dan curah hujan yang tinggi. Fauna tanah adalah salah satu komponen penting dalam tanah. Keberadaannya sangat berperan terhadap perbaikan sifat-sifat tanah, baik sifat fisik, kimia, dan biologi tanah. Penelitian mengenai fauna tanah dan perannya terhadap fungsi-fungsi di dalam ekosistem tanah masih belum banyak dilakukan di Indonesia. Tujuan dari penelitian ini untuk mempelajari populasi yang meliputi jumlah dan biomassa, serta keragaman fauna tanah pada 3 tipe penggunaan lahan yang berbeda di desa Situgede kecamatan Bogor Barat.
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Judul: Analysis Biological and Chemical Characteristics of Soil in Several Types Land Use in Bogor Regency
Abstrak: Penggunaan lahan di Kabupaten Bogor memiliki vegetasi beragam dan karakteristik tanah berbeda. Karakteristik tanah memiliki pengaruh terhadap pengelolaan lahan. Keberadaan mikrob tanah dapat digunakan sebagai bioindikator kesuburan tanah. Kesuburan tanah dihasilkan oleh interaksi sifat fisik, kimia, dan biologi tanah. Penelitian ini bertujuan mengetahui karakteristik sifat biologi dan kimia tanah pada berbagai tipe penggunaan lahan di Kabupaten Bogor. Pengambilan sampel tanah dilakukan dengan menggunakan metode purposive random sampling dengan 5 kali ulangan. Lokasi penelitian meliputi delapan penggunaan lahan yaitu kebun bambu, kebun jabon, kebun sengon, kebun lamtorogung, tegalan, tanaman sereh wangi, kebun buah naga, dan kebun pisang. Hasil analisis menunjukkan lahan pisang dan buah naga memiliki kadar C-organik, N-total tertinggi dan populasi fungi tertinggi yaitu 3,78 x 105 CFU/gram. Sedangkan pada lahan di Kecamatan Jasinga yang memiliki hara dan populasi fungi yang cukup dan populasi Azotobacter tertinggi yaitu pada lahan lamtorogung dapat diusahakan untuk tanaman perkebunan yang lebih memiliki nilai jual lebih tinggi seperti pada lahan tegalan dapat ditanami pohon karet, kelapa sawit, atau kakao., Land use in Bogor Regency has diverse vegetation and different soil characteristics. Soil characteristics have an influence on land management. The presence of soil microbes can be used as bioindicators of soil fertility. Soil fertility is produced by the interaction of physical, chemical, and biological properties of the soil. This study aims to determine the characteristics of the biological and chemical properties of soil in various types of land use in Bogor Regency. Soil sampling using the purposive random sampling method with 5 repeats. The research location includes eight land uses, namely bamboo, jabon, sengon, lamtorogung gardens, moorings, fragrant lemongrass plants, dragon fruit orchards, and banana gardens. The results of the analysis showed that banana and dragon fruit fields had the highest C-organic, N-total levels and the highest fungi population of 3,78 x 105 CFU/gram. Meanwhile, on land in Jasinga District which has sufficient nutrients and fungi populations and the highest Azotobacter population, namely on lamtorogung land, it can be cultivated for plantation crops that have a higher selling value, such as in moorlands that can be planted with rubber trees, oil palm, or cocoa.
Keyword: Azotobacter sp., microbes, soil fertility, total fungi

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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