Judul: Identification of Bioactive Compounds of Hemiplecta humphreysiana Lea, 1840 Mucus and Its Potential as An Inhibitors of Tyrosinase and Elastase In Silico
Abstrak: Lendir dari beberapa spesies keong telah diketahui bioaktivitasnya sebagai anti-tirosinase dan anti-elastase. Namun, keong darat spesies Hemiplecta humphreysiana Lea, 1840 native Indonesia belum diketahui potensi bioaktivitasnya sebagai inhibitor tirosinase dan elastase. Penelitian ini bertujuan mengidentifikasi senyawa bioaktif lendir H. humphreysiana Lea, 1840 serta memprediksi bioaktivitasnya sebagai inhibitor tirosinase dan elastase secara in silico. Metode penelitian meliputi produksi lendir keong, identifikasi senyawa bioaktif dengan instrumen UPLC-MS/MS, preparasi ligan dan reseptor, validasi penambatan molekuler, penambatan molekuler dengan Autodock Vina, visualisasi dan analisis interaksi molekul, serta prediksi bioavailabilitas dan toksisitas ligan. Hasil penelitian menunjukkan dari 34 senyawa bioaktif lendir H. humphreysiana Lea, 1840 hasil analisis UPLC-MS/MS, terdapat 12 dan 11 senyawa masing-masing berpotensi menginhibisi tirosinase dan elastase. Indoleacrylic acid dan withanone masing-masing merupakan senyawa potensial terbaik sebagai inhibitor tirosinase dan elastase, Mucus from several species of snails has been known to have bioactivies as anti-tyrosinase and anti-elastase. However, the land snail species Hemiplecta humphreysiana Lea, 1840, a native Indonesian snail, is not yet known for its potential bioactivity as a tyrosinase and elastase inhibitors. This study aimed to identify bioactive compounds in the H. humphreysiana Lea, 1840 mucus and to predict their bioactivities as tyrosinase and elastase inhibitors in silico. Research methods included production of snail mucus, identification of bioactive compounds using UPLC-MS/MS instrument, ligand and receptors preparation, validation of molecular docking, molecular docking with Autodock Vina, visualization and molecular interaction analysis, followed by bioavailability and toxicity ligand prediction. The results showed out of 34 bioactive compounds in the H. humphreysiana Lea, 1840 mucus from UPLC-MS/MS analysis, are respectively 13 and 12 potential tyrosinase and elastase inhibitors compounds. Indoleacrylic acid and withanone, respectively, are the best potential compounds as tyrosinase and elastase inhibitors
Keyword: elastase, Hemiplecta humphreysiana mucus, in silico, tyrosinase, UPLC-MS/MS

Judul: Identification of Bioactive Compounds of Moringa oleifera Leaves and Its Potential as Pancreatic Lipase Inhibitor In Silico
Abstrak: Obesitas merupakan penyakit kronis multifaktoral yang berbahaya bagi kesehatan karena memiliki komplikasi terhadap penyakit-penyakit degeneratif sehingga perlu dilakukan langkah pengobatan yang tepat untuk menanggulangi kasus obesitas. Sebuah obat antiobesitas orlistat yang disetujui FDA merupakan inhibitor ireversibel lipase pankreas yang masih memiliki efek samping gastrointestinal. Oleh karena itu, penelitian terhadap senyawa antiobesitas alami diperlukan. Kelor mengandung banyak senyawa alami dan berpotensi sebagai antiobesitas. Penelitian bertujuan mengidentifikasi senyawa aktif dalam ekstrak etanol kelor (Moringa oleifera) dan menentukan senyawa yang berpotensi menginhibisi aktivitas enzim lipase pankreas secara in silico. Metode LC MS/MS-QToF digunakan untuk mengidentifikasi senyawa aktif, dilanjutkan dengan penambatan molekuler secara penapisan virtual dengan aplikasi YASARA Structure. Sebanyak 18 senyawa aktif dari golongan flavonoid, fenolik, dan saponin berhasil diidentifikasi berdasarkan hasil analisis LC MS/MS-QTOF dari ekstrak etanol 70% kelor. Senyawa aktif kelor hasil LC MS dan 72 senyawa hasil studi literatur digunakan sebagi ligan uji dalam penelitian ini. Hasil penelitian menunjukkan bahwa ada sebanyak 32 dari 90 ligan uji berpotensi sebagai inhibitor lipase pankreas. Kaemferol, apigenin, dan luteolin merupakan tiga senyawa terbaik yang berpotensi sebagai inhibitor lipase pankreas., Obesity is a chronic multifactorial disease that is dangerous to health because it has complications for degenerative diseases, Therefore, an appropriate treatment step requires to be taken to overcome obesity cases. An FDA-approved anti-obesity drug, orlistat, is an irreversible inhibitor of pancreatic lipase that still has gastrointestinal side effects. Thus, research on natural anti-obesity compounds is needed. Moringa contains a lot of natural compounds and has potential as anti-obesity. This research aims to identify the bioactive compounds in the ethanolic extract of Moringa oleifera and determine the potential inhibitor of pancreatic lipase in silico. The LC MS/MS-QTOF method was used to identify the bioactive compound, followed by molecular docking based on virtual screening using the YASARA Structure. Out of 18 active compounds were identified based on the flavonoid, phenolic, and saponin results of the LC MS/MS-QTOF from M. oleifera ethanol 70% extract. All identified Moringa bioactive compounds from LC MS and 72 bioactive compounds from literature were used as experimental ligands in this study. The results showed that 32 out of 90 ligands have potential as pancreatic lipase inhibitor. Kaemferol, apigenin, and luteolin are the most potential of pancreatic lipase inhibitor.
Keyword: antiobesitas, inhibitor lipase pankreas, in silico, Moringa oleifera

Judul: Implementasi Space Time Composite (STC) Data Model Menggunakan Model Data Relasional
Abstrak: The data model is a concept that describes the data stored in the database and the relationship between the data to the user in logic. Development of data models is very important in the design of the database as the basis of the stage of database design to be made. This study aims to apply the concept of Space Time Composite (STC) on the Model Data storage vector data types that can store enough information and describes the process of drafting objects and objects spatiotemporal evolution over time. From the results of the test queries on the data model that have been created, queries in a system are acquired that can help display the information preparation of objects from time to time and know the history of the object polygon and arc that has undergone changes in topology.
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