Judul: Produksi dan Karakterisasi Protease Ljocillus Sp. Galur BBU-32 Asal Saluran Pencernaan Parastronuuems niger
Abstrak: Sebanyak 42 isolat bakteri proteolitik diisolasi dari saluran pencemaan ilean bawal hitam (Paroslromateus niger). Dipilih 4 isolat yang memiliki indeks proteolitik tertinggi dengan penampakan morfologi yang berbeda untuk diukur aktivitas proteasenya. Terpilih isolat 8BU-32 yang memiliki aktivitas spesifik tertinggi dan diidentifikasi sebagai Bacillus sp. Media yang optimum bagi pertumbuhan Bacillus sp. galur 8BU-32 terdiri alas peptOR 0.50/0. pati terlamt 0.10/0. susu skim 0.5% dan MgCh 0.1%. Pada media yang dimodifikasi im, protease Bacillus sp. galur 8BU-32 menunjukkan aktivitas maksimum pada saat biakan berumur 24 jam. Dengan menggunakan media NB yang mengandung susu skim 0.5% dan glukosa 0.1% aktivitas protease Bacillus sp. gator BBU- 32 maksimum dicapai pada suhu SOoC dan pH 7.6. Sedangkan pada media yang dimodifikasi aktivitas protease maksimum dicapai pada suhu SOoC dan pH 8. Penambahan 5 mM Na + dan K+ mampu meningkatkan aktivitas protease masing-masing sebesar 100.4% dan 103.7% sedangkan penambahan 5 mM EDT A dan PMSF menurunkan aktivitas protease, masing-masing tersisa sebesar 16.71%dan 5.47%.
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Judul: Karakterisasi dan produksi protease ekstraselular Bacillus sp.galur BKU-10 dari saluran pencernaan Epinephelus tauvina
Abstrak: Bacillus sp. galur BKU-10 diisolasi dari saluran pencernaan ikan kerapu lumpur (Epinephelus tauvina). Aktivitas protease ekstrak kasar yang dihasilkan Bacillus sp. galur BKU-10 dihitung dan dikarakterisasi pada jam ke-32 setelah inokulasi pada media NB + susu skim 0.5% dan jam ke-33 pada media modifikasi, yang terdiri atas glukosa 0.5% sebagai sumber karbon, ekstrak khamir 0.3% sebagai sumber nitrogen, susu skim 0.5%, dan garam anorganik CaCl₂ 0.1%. Protease Bacillus sp. galur BKU-10 mempunyai karakteristik sebagai protein teradaptasi panas, yaitu antara 30 sampai 60°C dan memiliki aktivitas optimum pada suhu 50°C. Enzim ini memiliki pH optimurn 8 pada media NB+ susu skim 0.5% dan pH 7.6 pada media modifikasi. Pengaruh penambahan kation divalen seperti Ca, Mg, Cu, Mn, Zn, and Co² 5 mM menurunkan aktivitas protease. Senyawa inhibitor-metaloprotease, etilenadiamina tetraasetat (EDTA) 5 mM tidak berpengaruh pada aktivitas protease. Enzim ini dihambat oleh inhibitor-serin, fenilmetilsulfonil fluorida (PMSF) dengan konsentrasi 5 mM.
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Judul: Thermal Characteristics and Dynamics of Tropical Cyclone over Western North Pacific Ocean: A Case Study of Typhoon Songda (Chedeng) 2011
Abstrak: The frequent occasion of Tropical Cyclone (TC) over Western North Pacific (WNP) Ocean lies from 10˚N to 26˚N and 121˚E to 170˚E. Sources of TC development come from warm ocean temperature where latent heat is released during condensation processes and subsequently concentrated in the boundary layer. Relative humidity at 850-700 hPa is responsible to produce the most intense storm. Meanwhile, weak vertical wind shear between 850-200 hPa is one of the dynamical factors that lead to the development of TC. Pressure level of 850-200 hPa is chosen to minimize the thermodynamic effect in the lower troposphere. Typhoon Songda (Chedeng in the Philippines) or TSC lasted from May 18th to 30th, 2011 and strengthened into powerful Super Typhoon stage between May 24th and 27th. Long curving track and the exhibition of all sequences of TC life cycle, TSC is then considered as an ideal case of TC. The objective of the study with case of TSC is to investigate the role of thermal characteristics and dynamics on TC intensity. The research is carried out by using Advanced Research of Weather Research and Forecasting model (WRF ARW model) version 3.3. Two domains are employed in the model. The first domain situate from 02˚N to 40˚N and 118˚E to 149˚E and cover spatial resolution () of 33.1 km. The second domain situate from 09˚N to 27˚N and 119˚E to 132˚E with of 11 km. Timestep of the model () is 120 seconds. The model is supported by the following physical parameterization, i.e., Yonsei University Scheme (YSU) in the boundary layer, Kain-Fritsch scheme (KF) in the cumulus, and WRF Single-Moment 3-class scheme in the microphysics. Initial meteorological condition prior to the formation of the cyclone is generated using WRF ARW standard-release model. Sensitivity of the model output is obtained from a selected single-parameter change scenarios, namely, 10 % to 20 % decrease and increase of wind shear initial data at 850-200 hPa, and by 10 % to 20 % decrease of relative humidity initial data at 850-700 hPa. The result showed that the negative correlations between wind shear and intensity are found in wind shear scenarios. Wind shears of 10 ms-1 which lead to strengthening of the wind intensity in the early period of Typhoon development are found in wind shear scenarios. The effect of wind shear changes on wind intensity is statistically significant in the 20 % wind shear scenarios. During mature stage, weak wind intensity occurred when wind shear increase as given in the WRF standard-release and the 20 % decreasing scenario. Different situation is found in the 20 % increasing scenario where wind shear tend to weak and lead to strong wind intensity at this stage. Meanwhile, the reduction of moisture supply at 850-700 hPa resulting from 20 % decreasing relative humidity play a major role in the weakening of wind intensity and reduces mechanical energy of the cycle process as much as 300 J kg-1.
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