Judul: Perakitan Vektor Ekspresi Protein G Subunit α dari Kedelai (Glycine max) Kultivar Slamet
Abstrak: Heterotrimeric G protein consists of three subunits α, β, and γ. Activation of Gα protein is induced by biotic or abiotic stress. It is supposed that Gα protein has an important role in the Al tolerance in soybean cv. Slamet. To study the role of Gα in the tolerance to Al stress in the plant, the gene encoding for Gα had to be expressed in the transgenic plant. For this reason, the expression vector of this Gα gene has to constructed. So, this research had an objective to construct the expression vector of Gα gene of soybean cv Slamet. The expression vector pMSH1- Gα was succesfully constructed by inserting 1380 bp Gα gene into pMSH1 plasmid under 35S CaMV promoter, so this size of recombinant plasmid is 15380 bp. This recombinant plasmid was succesfully introduce into Escherichia coli DH5α strains.
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Judul: Identifikasi Secara Molekular Kandidat Mutan Gα dari Kedelai Kultivar Slamet Berdasarkan mRNA
Abstrak: Heterotrimeric G proteins composed of α, β, and γ subunits, are bound to the inside surface of the cell membrane. Gα proteins will be activated by extracellular stimuli (biotic or abiotic stress). It is presumably Gα proteins playing an important role in aluminum (Al)-stress tolerance. Soybean cv. Slamet was considered as a tolerant plant to Al and has a single copy of Gα gene. Gamma irradiation to soybean cv. Slamet seeds caused a random chromosomal mutation of second generation plants (designated as M2). The Gα mutant candidates were selected based on stomatal closure in the daylight and dwarf. Gα mutant candidates M4, number 338 and 103 have severe root cell tissues damage compared to non mutant ones. Plant number 338/3S1.174/12/14 (M4) and 103/3S3.103/8/5/1/4 (M5) have a Gα gene mutation at the U3-L4 sequences (exon 5 – exon 11). Whereas, plant number 344/3S1.196/14/1/8 (M4) is assumed as Gα gene mutant. All of those plants were replanted until sixth generation. The candidates of Gα mutant selected have more than fifty percent of closed stomata and dwarf. Four number of M5 plants and three number of M6 plants were selected for Gα mutant identification based on mRNA analysis. The fifth generation plant number 344/3S1.196/14/1/8/7 were decreased its Gα gene expression at L5-L4 sequences (exon 7 – exon 11). The M6 generation plant number 344/3S1.196/14/1/8/7/5 have mutation in Gα gene at L1-L3 sequences (exon 2 – exon 6). A major contributing factor to decreased expression levels of Gα gene may be due to mutation of Gα gene promotor. Three other of M5 plants were assumed as Gα mutant. M6 plant number 103/3S3.103/8/5/1/4/8 has mutation at L3-L2 sequences (exon 6 – exon 15) and number 103/3S3.103/8/5/1/4/9 has mutation at U3-L4 sequences (exon 5 – exon 11). The decreasing of gene expression caused by mutation in those plants was indicated by the less pronounced DNA bands on agarose gel than that of non mutant plants.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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