Judul: Pengaruh Nilai dan Perilaku Investasi Anak terhadap Kesejahteraan Anak Usia Prasekolah.
Abstrak: Kesejahteraan anak usia prasekolah merupakan hal yang penting untuk diperhatikan karena pada masa tersebut anak sedang berada pada masa kritis yang sangat menentukkan keberhasilan pertumbuhannya. Nilai dan perilaku investasi anak adalah faktor yang berkontribusi dalam mewujudkan kesejahteraan anak. Penelitian ini bertujuan untuk menganalisis pengaruh nilai dan perilaku investasi anak terhadap kesejahteraan anak usia prasekolah. Desain penelitian yang digunakan adalah cross sectional study yang dilakukan di Kelurahan Babakan Pasar, Kecamatan Bogor Tengah, Jawa Barat. Penarikan contoh menggunakan metode simple random sampling sejumlah 98 keluarga yang memiliki anak usia 3-6 tahun. Data yang digunakan adalah data primer yaitu data yang dikumpulkan melalui metode wawancara dengan alat bantu kuesioner. Analisis data yang digunakan adalah regresi linear berganda. Hasil penelitian menunjukkan bahwa nilai anak, perilaku investasi anak, dan kesejahteraan anak terkategori sedang. Perilaku investasi anak berpengaruh positif signifikan, sedangkan nilai anak dimensi sosial berpengaruh negatif signifikan terhadap kesejahteraan anak. Selain itu, ditemukan juga bahwa usia anak berpengaruh positif signifikan terhadap kesejahteraan anak.
Keyword: nilai anak, investasi anak, kesejahteraan anak, anak usia prasekolah

Judul: Pengaruh Nilai Anak Terhadap Perilaku Investasi Anak pada Keluarga Miskin dan Tidak Miskin
Abstrak: Children investment behavior is on important factor to reach qualified children outcomes. This research was to analyze influence of value of children on children investment behavior of poor and non-poor family, involving 28 poor family and 27 non-poor family. Samples were famillies that have children in 1st grade and 2nd grade elementary school that were chosen randomly from the population who lived in Kotabatu Village, Ciomas Sub District, Bogor District. Data were collected by interviewing the mothers and using questionnaire. This research showed that there was positive and significant influence of wife’s education level, family poverty category, and social value of children on children investment behavior. Furthermore, analysis result showed that the number of schooled children and economic value of children had positive and significant influence on children investment expenditure allocation
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Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable