Judul: Pengaruh Penggunaan Getaran Suara Oan Pemberian Nutrisi Terhadap Laju Pertumbuhan Tanaman Teh (Camellia sinensis (L) O. Kuntze) Di Pembibitan
Abstrak: Penelitian ini dilaksanakan di Kebun Pusat Penelitian Teh dan Kina Gambung, Bandung pada bulan Desember 2000 sampai Mei 2001. Penetitian ini bertujuan untuk mengetahui pengaruh penggunaan getaran suara dan pemberian nutrisi terhadap laju pertumbuhan tanaman teh di pembibitan.
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Judul: Pengaruh Pupuk Hayati Terhadap Pertumbuhan Dua Klo Tanaman The (Camellia sinesis (L) O. Kuntze) Belum Mengasilkan
Abstrak: Percobaan ini bertujuan untuk mengetahui pengaruh pupuk hayati terhadap pertumbuhan dua k10n tanaman teh belum menghasilkan. Percobaan ini dilakukan mulai bulan Juli sampai dengan bulan November 2000 di Kebun Percobaan Fakultas Pertanian IPB Cikabayan, Darmaga, Bogor. Dalam percobaan ini digunakan Rancangan Petak Terbagi (RPT) dengan 2 faktor perlakuan yang disusun secara faktorial. Sebagai petak utama, jenis k10n yang terdiri atas RB 3 (K1) dan Gambung 5 (K2), sedangkan sebagai anak petak terdiri atas : 6.25 g EMAS + 50 % dosis anjuran pupuk anorganik (d.a.p.a) (PI), 10 ml EN4 + 50 % d.a.p.a (P2), 25 g OST + 50 % d.a.p.a (P3), 20 ml Soils Plus + 50 % d.a.p.a (P4) dan 12.5 g Urea. + 5 g TSP + 5 g KCI (Ps). Dengan demikian terdapat 10 kombinasi perlakuan dan setiap kombinasi perlakuan diulang 3 kali, sehingga seluruhnya terdapat 30 satuan percobaan. Masing-masing satuan percobaan terdiri atas 10 tanaman sehingga jumlah seluruhnya ada 300 tanaman. Peubah yang diamati meliputi tinggi tanaman, jumlah daun, diameter batang, jumlah tunas, panjang tunas, yang diamati setiap dua minggu mulai bulan Agustus sampai dengan bulan November 2000.
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Judul: Identifikasi Molekuler Kompleks Spesies Himantura uarnak (Batoidea: Dasyatidae) di Indonesia.
Abstrak: Stingrays are one of the cartilaginous fish, apart of sharks and skates, and one of Indonesia's fishery resources. Almost of elasmobranch fishes are economically important fish. The refined products and live specimens of this fish are a lot of traded. Himantura uarnak, H. undulata and H. leoparda are stingray species in Himantura and Dasyatidae family, which are the most popular stingrays in Indonesia waters. The stingrays are major target species were used as a consumable product and trading products. Levels of species diversity of cartilaginous fish in Indonesia are very high, and also Indonesia is listed as one of the countries that use elasmobranch resources the highest in the world. Generally, this study aims to identify each species of H. uarnak species complex through molecular approaches. While specifically, this study aims to estimate the evolutionary process of species divergence and to determine the relationship between species. The methods were used in this study included field sampling, sample collection, and specimen identification, collection of genetic samples, analysis of genetic samples and data analysis . Size polymorphism nuclear DNA markers (intron EPIC) were used. Polymorphism alleles translated into genotypes and alleles frequency of five introns loci. The results of Bayesian analysis and Correspondence Analysis (CA) produced informative diagram of the population structure showed 4 different clusters. Cluster 1 and 4 were H. leoparda, Cluster 2 indicated as H. undulata and the Cluster 3 was H. uarnak. Cluster 1 was a variant of Cluster 4, in this time called as H. leoparda var.1. Separation of four clusters according to nuclear DNA need to be proved by mitochondria DNA maker by cytochrome c oxidase subunit I (COI) and cytochrome b (cyt b). Molecular approaches were using mitochondrial DNA gene cytochrome c oxidase subunit I (COI) and cytochrome b produced 4 main groups (Group I, II, III and IV). Both of these molecular approaches showed each individual of groups were in consistent clade and the main group. Hence the H. uarnak species complex in Indonesian waters were divided into 4 main groups, and each was a particular species. The Group I and IV were the two group species that have been released as H. leoparda and Group I was H. leoparda var. 1 identified as cryptic species. Group II was one individual who grouped as H. undulata. Group III was one of other group that identified as H. uarnak and in accordance with the morphological characteristic through spotting pettern. Morphological approach to identified the stingrays of Himantura uarnak species complex clearly less accurate than the molecular approaches. Morphological approach should not be used to identify a species complex in stingrays without helped by other approaches.
Keyword: Himantura uarnak species complex, intron EPIC, cyt b, COI, spotting pattern