Judul: Marigold (Tagetes erecta) Callus Induction on Several In Vitro Culture Media Composition
Abstrak: ALDYNO JULIAN. Induksi Kalus Marigold (Tagetes erecta) pada Beberapa Komposisi Media Kultur In Vitro. Dibimbing oleh DEWI SUKMA dan SHANDRA AMARILLIS. Marigold merupakan tanaman dari keluarga Asteraceae yang tersebar luas di seluruh dunia dengan berbagai spesies dan biasa digunakan sebagai tanaman hias dalam pot maupun sebagai karangan bunga. Permintaan konsumen yang tinggi akan tanaman ini, menyebabkan diperlukan upaya untuk meningkatkan produksi dan kualitas. Kultur jaringan merupakan teknik perbanyakan tanaman yang efektif agar dapat menghasilkan tanaman dalam jumlah yang besar dalam waktu yang cepat, serta sifat yang sama dengan induknya. Perbanyakan dalam kultur jaringan dapat dilakukan melalui jalur organogenesis dan embriogenesis somatik. Tahap awal dari organogenesis maupun embryogenesis somatic dalam kultur jaringan adalah induksi kalus. Pemberian zat pengatur tumbuh, vitamin maupun asam amino dalam media dibutuhkan untuk mendorong inisiasi dan pertumbuhan kalus. Kalus yang terbentuk kemudian akan berkembang dan beregenerasi menjadi tanaman lengkap. Penelitian ini bertujuan mempelajari respon pertumbuhan kalus dari eksplan hipokotil marigold varietas Vanilla Gayatri pada beberapa komposisi media kultur jaringan. Penelitian dilaksanakan di Laboratorium Kultur Jaringan 1, Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor. Percobaan ini dilaksanakan pada bulan Januari-Maret 2020. Penelitian ini menggunakan Rancangan Kelompok Lengkap Teracak (RKLT) dengan satu faktor perlakuan. Hasil dari penelitian ini menunjukkan respon pembentukan kalus pasca perlakuan selama tiga minggu terbaik ditemukan pada komposisi media (MSO + 2 ppm BAP+ 0,5 ppm NAA + 50 ml air kelapa) + thiamin 2,0 µM + biotin 2,0 µM) dengan tekstur remah dengan warna hijau. Pengujian dan pengamatan lebih lanjut masih dibutuhkan untuk melihat kemampuan organogenesis tunas dari kalus. Kata kunci: hipokotil, kalus, kultur jaringan tanaman, vitamin, ALDYNO JULIAN. Marigold (Tagetes erecta) Callus Induction on Several In Vitro Culture Media Composition. Supervised by DEWI SUKMA and SHANDRA AMARILLIS. Marigolds are plants from the Asteraceae family that are widely distributed throughout the world with various species and are commonly used as ornamental plants in pots and as bouquets. The high consumer demand for this plant requires efforts to increase production and quality. Tissue culture is an effective plant propagation technique that can produce a large number of plants in a short time, as well as the same properties as the parent. Propagation in tissue culture can be done through organogenesis and somatic embryogenesis. The initial stage of organogenesis and somatic embryogenesis in tissue culture is callus induction. The provision of growth regulators, vitamins and amino acids in the media is needed to encourage callus initiation and growth. The callus formed will then develop and regenerate into a complete plant. This research aims to study the callus growth response of hypocotyl explants of marigold variety Vanilla Gayatri on several compositions of tissue culture media. The research was conducted at Tissue Culture Laboratory 1, Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University. The experiment was conducted from January to March 2020. This study used a completely randomized group design (RKLT) with one treatment factor. The results of this study showed that the best callus formation response after treatment for three weeks was found in the media composition (MSO + 2 ppm BAP + 0.5 ppm
Keyword: callus, hypocotyl, plant tissue culture, vitamin

Judul: Growth and Flowering Response of Two Clones of Marigold (Tagetes erecta L.) to Frequency of Pinching and Fertilization
Abstrak: Marigold (Tagetes erecta L.) merupakan tanaman herba hias yang berasal dari famili Asteraceae. Bunga marigold memiliki bentuk yang unik serta warna yang indah sehingga biasa digunakan sebagai tanaman hias dalam pot maupun sebagai karangan bunga. Marigold memiliki potensi untuk dikembangkan karena memiliki nilai ekonomis yang tinggi dan bunganya memiliki banyak kegunaan. Salah satu tindakan budidaya yang dapat meningkatkan pertumbuhan dan kualitas bunga adalah dengan perlakuan pinching dan pemupukan. Penelitian dilaksanakan pada bulan Desember 2022 - Juni 2023 di Kebun Percobaan CV. Benih Dramaga, Kecamatan Dramaga, Kabupaten Bogor. Penelitian ini menggunakan Rancangan Kelompok Lengkap Teracak (RKLT) faktorial dengan taraf dua faktor yaitu frekuensi pinching dan frekuensi pemupukan AB mix. Faktor pertama adalah frekuensi pinching dari tiga taraf yaitu: 1 hari sekali (A1), 2 hari sekali (A2), dan 3 hari sekali (A3). Faktor kedua adalah frekuensi pemupukan dari dua taraf yaitu 2x/minggu (B1) dan 3x/minggu (B2). Pupuk yang digunakan pada penelitian ini adalah pupuk AB mix. Varietas marigold yang digunakan yaitu Sudamala Emas 1 sebagai tanaman utama dan Maharani sebagai tanaman pembanding. Hasil penelitian dari pengaruh frekuensi pinching, frekuensi pemupukan serta interaksi pinching dan pemupukan menunjukan pengaruh yang tidak berbeda nyata terhadap tinggi tanaman, jumlah bunga per tanaman, bobot bunga per tanaman, bobot bunga per kuntum, diameter bunga, dan Umur 50% berbunga., Marigold (Tagetes erecta L.) is an ornamental herbaceous plant from the Asteraceae family. Marigold flowers have a unique shape and beautiful colors so they are usually used as ornamental plants in pots or as flower bouquets. Marigolds have the potential to be developed because they have high economic value and the flowers have many uses. One of the cultivation measures that can improve the growth and quality of flowers is pinching and fertilizing. The research was carried out in December 2022 - June 2023 at the CV. Benih Dramaga, Perumahan IPB Alam Sinarsari Dramaga, Kecamatan Dramaga, and Kabupaten Bogor. This research used a factorial Randomized Complete Block Design (RCBD) with two factor levels, namely pinching frequency and AB mix fertilization frequency. The first factor was the frequency of pinching from three levels, namely: once a day (A1), once every 2 days (A2), and once every 3 days (A3). The second factor was the frequency of fertilization at two levels, namely 2x/week (B1) and 3x/week (B2). The fertilizer used in this research was AB mix fertilizer. The marigold variety used was Sudamala Emas 1 as the main variety and Maharani as the comparison. The results of the study of the effect of pinching frequency, fertilization frequency and the interaction of pinching and fertilization showed an effect that was not significantly different from plant height, number of flowers per plant, flower weight per plant, flower weight per flower, flower diameter, and 50% flowering time.
Keyword: marigold, pinching, pemupukan

Judul: Truncated Stop Loss as a Optimal Solution of Reinsurance Agreement in One-Period Model
Abstrak: Reinsurance is one of risk management efforts of an insurance company, which is called the cedent. Similar with insurance, reinsurance constitutes the cedent to pay the premium. We consider one-period reinsurance model and derive a rule which minimizes the ruin probability of the cedent for a fixed reinsurance premium. Therefore, it is needed to construct an optimal reinsurance agreement for the cedent. The premium is calculated according to four different principles, i.e. economic, generalized zero-utility, Esscher, and mean-variance principles. Stop loss is a reinsurance contract which protect the insurance company for losses that exceed a certain amount. The result of this research shows that a truncated stop loss is an optimal agreement in the class of all reinsurance contracts with various premiums calculated using above mentioned principles.
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