Judul: Dinamika Perubahan Penggunaan Lahan dan Kecukupan Usahatani di Kabupaten Subang
Abstrak: Subang regency is located adjacent to Jakarta on the north coast of West Java. This strategic location has positioned Subang for having an added value of easy access. Therefore, it is exposed to increasing regional growth and development. Approximately 11,250 ha of the area have been allocated in a spatial plan as industrial area. The allocation causes land alteration, primarily in agricultural fields. Considering its significant impact, land conversion needs to be controlled. This research aims to observe the patterns of agricultural land use change in Subang during 2007-2013, as well as to study factors affecting land conversion by using logistic regression and to identify farmer’s interest to maintain their agricultural land. Analysis of land use conversion in 2007-2013 indicates that mixed garden and built-up increased considerably. These rises consume other land use types, including plantations, rice fields, and dry land agriculture. Other land use types remained constant. Logistic regression analysis concluded that the change of agricultural fields to built-up was influenced by several factors, including soil type, spatial plan and distance to the capital city. While factors affecting the interests of farmers were net agricultural income, total production and landrent. The Markov Chain accuracy in predicting land use of 2013 in Subang regency as much as 96.4%. This method is fairly accurate in predicting the use of mangroves, ponds, and forests
Keyword: Subang, farming interest, markov chain, logistic regression, land use change

Judul: Perubahan Penggunaan/Penutupan Lahan dan Faktor yang Mempengaruhinya (Studi Kasus: Kota Sukabumi, Jawa Barat)
Abstrak: The strategic position of Sukabumi City between Jabodetabek and Bandung Raya intensified the development in this region. It implied to the high of land use/land cover change (LUCC) in Sukabumi City. We utilized IKONOS in 2010 and 2012 to determine LUCC pattern. Furthermore, multiple regression was employed to find out affecting factors of LUCC. Skalogram analysis was then utilized to find out the level of regional development, descriptive analysis to correlate LUCC and level of regional development, and benchmarking of current utilization to evaluate the conformity of current land use/land cover with Spatial Plan (RTRW). The spatial analysis showed that within two years, built up area of Sukabumi increased at 2.8% while rice field and unproductive land decreased at 1.2% and 1.9% respectively. Factors affecting of LUCC in Sukabumi City were the availability of land; distance to CBD, allocation of Spatial Plan (RTRW); distance to economic, education, and medical facilities; regional development index; and the increase of population. Unproductive land change into agricultural land occured in 2nd hierarchy and 3rd hierarchy, whereas agricultural land change into built up area and unproductive land change into built up area occured in all of the hierarchy. Land use/land cover in 2012 conformed with RTRW was 36.6%, while 45.1% was undeveloped, and 2.3% was unconfirmed the plan.
Keyword: regional development, factors of change, land use/land cover change

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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