Judul: Analisis biaya produksi dan minimisasi biaya persediaan bahan baku (Studi kasus di PT. Industri Sandang II Persero Patun Muriateks Kudus)
Abstrak: Perkembangan industri tekstil yang cukup pesat, mendorong masuknya perusahaan-perusahaan baru. Hal ini menimbulkan masalah : (1) semakin tingginya biaya produksi, (2) terjadinya perubahan kemampulabaan, (3) perlunya penanganan persediaan bahan baku yang lebih baik, (4) juga perlunya pedoman dalam pengelolaan bahan baku tekstil.
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Judul: Analisis Pengendalian Persediaan Bahan Baku Kemeja di PT Unie Sakinah Busana
Abstrak: Manajemen pengendalian persediaan bahan baku digunakan untuk mengelola penggunaan bahan baku agar penggunaannya optimal, sehingga tidak terjadi kekurangan maupun kelebihan bahan baku yang menyebabkan terjadinya peningkatan biaya persediaan. PT Unie Sakinah Busana memproduksi berbagai macam kebutuhan fashion, salah satunya adalah kemeja. Tujuan dari penelitian ini adalah : 1. Memahami manajemen dan permasalahannya sistem persediaan bahan baku saat ini pada PT Unie Sakinah Busana. 2. Mengidentifikasi bahan baku yang paling kritis atau memiliki prioritas utama karena memiliki dampak yang besar terhadap proses produksi di PT Unie Sakinah Busana. 3. Analisis efisiensi biaya penyimpanan bahan baku pada PT Unie Sakinah Busana. Untuk mengoptimalkan persediaan bahan baku yang digunakan, maka dilakukan perhitungan dengan menggunakan analisis ABC, dan metode Economic Order Quantity (EOQ) dengan menggunakan software POM for windows 3. Berdasarkan perhitungan analisis ABC, dari sebelas bahan baku penyusun, terdapat dua bahan baku yang berada pada kategori A yaitu, bahan polyester dan bahan cotton. Perhitungan total biaya persediaan bahan baku dala setahun keseluruhan untuk kedua bahan baku tersebut dengan menggunakan metode EOQ dengan bantuan software POM sebesar Rp. 4 139 866 000, nilai tersebut lebih kecil dibandingkan perhitungan perusahaan yang sebesar Rp. 4 145 424 009, sehingga perusahaan dapat melakukan penghematan sebesar Rp. 5 558 009 dalam satu tahun.
Keyword: EOQ, Optimalisasi POM, Pengendalian Bahan Baku

Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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