Judul: Hubungan Antara Perilaku Menonton Film Percintaan Dengan Sikap Remaja Terhadap Perilaku Pacaran (Kasus Di Jakarta Timur: Sekolah Lanjutan Tingkat Pertama Negeri 99, Pulomas; Sekolah Menengah Atas Negeri 36, Rawamangun; dan Fakultas Ekonomi Universitas Negeri Jakarta, Rawamangun).
Abstrak: Film merupakan cermin atau jendela masyarakat di mana media massa itu berada sehingga nilai, norma, dan gaya hidup yang berlaku pada masyarakat akan disajikan dalam film yang diproduksi. Akan tetapi film juga mempengaruhi sikap dengan cara memperteguh, menciptakan, dan mengubah norma jika film terse but ditonton berulangkali. Dalam hal ini, menonton film merupakan perilaku penggunaan media yang dapat dilihat dari frekuensi menonton film dan jenis film yang ditonton. Kehadiran film terutama film percintaan yang menampilkan nilai. norma, dan tingkah laku pacaran yang mengarah kepada aktivitas seksual maka nilai, norma dan tingkah laku pacaran yang ditunjukkan dalam film tersebut akan diadopsi oleh remaja sehingga sikap remaja lebih permisif terhadap perilaku pacaran yang mengarah kepada aktivitas seksual. Perilaku menonton film percintaan dapat mempengaruhi sikap remaja terhadap perilaku pacaran. Bagaimanakah hubungan antara perilaku menonton film percintaan dengan sikap remaja terhadap perilaku pacaran? Oleh karena itu, tujuan penelitian ini adalah mengetahui perilaku menonton film percintaan remaja dan sikap remaja terhadap perilaku pacaran, serta menganalisa hubungan yang terjadi antara perilaku menonton film percintaan dengan sikap remaja terhadap perilaku pacaran.
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Judul: Persepsi, pengetahuan, dan perilaku menonton film layar lebar pada siswa SMA Negeri 3 Kota Bogor
Abstrak: The rapid development of movie industries over the past years makes producers compete in making more movies. Facts shows that Indonesian movie produces only think of profits alone, without regarding to the benefits and impact of a movie. This study aims to analyze the perceptions, knowledge, and behaviors on SMA 3 Bogor high school students towards watching movies.Respondents are SMA 3 Bogor students who had watched a movie in the past year.The study involved 100 students were selected conveniently. Data is collected through interviews using questionnaires. The data obtained were analyzed using descriptive and inference statistics. Results showed that students had a perception less amenable towards the movies with the level of knowledge in the middle category. In the meantime, the watching behavior of students are in the low category. Pearson correlation test results shows that the amount of students pocket money alone have a significant relationship towards the behavior of watching a movie.
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Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.