Judul: Pengembangan Model Pemanfaaatan Hutan Untuk Pertumbuhan Ekonomi Wilayah dan Peningkatan Kesejahteraan Masyarakat Secara Berkelanjutan
Abstrak: Fundamental law of forest notifies that deforestation was done by the forest concessionaires will influence to the traditional community around the forest, that is their activities, their living condition, and also to the regional (Jayapura) economic growth. Forest rehabilitation, guidance, and counseling activities in the community that was done by the forest concessionaires was not match to their objectives, thus the report documentatio~l was not fulfill to the target of this activities. Forest protection rules for the comm~~niwtye lfare have not shown significantly changes to the community and forest sustainability. Analysis with Social Accounting Matrix Model (SAM) was shown that the forest management in Jayapura had negative impact to the income of rural community, and indirectly to the economic growth of Jayapura regency. By micro approach shown the correlation between educa-t ion, informal institution, with community income and welfare. Thus, forest management needs participation from traditional community, goverment and forest concessionaires in term of economic growth and community welfare.
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Judul: Kelembagaan Lokal dalam Pengelolaan dan Pemanfaatan Sumberdaya Hutan (Kasus Masyarakat Nambluong di Kabupaten Jayapura, Papua)
Abstrak: The objective of this study was to describe the local institution 111 the management of forest resource and its implications for forest performance. This @ dy used a case study method. Data were collected through in-depht interviews, ~ctured interviews, observation and measurement. The result showed that the ~stitutional aspects in the forest management in the Nambluong community (") i-iiclude values, norms and lUles. The value of forest resources refers to the ~thropocentric orientation. The identified systems of knowledge and nOlms are ,rest categorization, cultivation system, descent groups, prohibition of cultivation ~ld cutting down trees at the riverside, prohibition of using certain types of wood, ~ohibition of stealing or destroying villages, farms and forests, as well as the ban ~ selling villages/farm land to outsiders. The lUles on the holding rights of forest ~sources refer to the rights to control and use as well traditional sanctions. These ~hts are based on the system of khinship and traditional leadership. Social & rms and lUle on the rights of the forest resources have implications to the :::::s iiistainable forest resource as the main sources of livelihood for the community. :::::s Qihese implications are demonstrated by the sustainable forest resource to o ~oduction of crops, dusun of sago and timber, which are have a ecologi function. k the secondary and primary forest areas, the measurement of perfOlmance found a good implication in performance. This performance is indicated by the sufficient number or density of plant species, which is of good category with the diversity of plant species at the moderate level. The institutional aspects that are tlUsted, obeyed and practiced in managing and utilizing forest have given the implications of a good management of forest resources. This is indicated by the clarity of tenure limits for the tang groups that have been recognized by the community, thus providing guaranteed security in utilizing forest. The regulation of the l!ilizing rights for the tang members can control the behavior of individuals who ~e forest resources excessively.
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Judul: Influenza Specific Immunoglobulin Y Purification from Egg Yolk with Sodium Chloride Precipitation Method
Abstrak: Imunoglobulin Yolk (IgY) yang terdapat dalam kuning telur telah banyak dimanfaatkan untuk imunoterapi dan imunodiagnostik. Salah satu kendala dalam pemurnian IgY dari kuning telur adalah pemisahan Ig dengan lemak, sehingga teknik pemurnian Ig selalu berkembang. Penelitian ini bertujuan untuk melakukan pemurnian IgY dari kuning telur dengan metode pengendapan menggunakan sodium chloride (NaCl) serta melakukan karakterisasi IgY spesifik influenza. Sodium chloride dipilih karena bahan kimia ini relatif murah, ramah lingkungan, dan tidak merusak imunoaktivitas IgY. Kuning telur yang digunakan dalam penelitian ini berasal dari telur ayam layer yang divaksinasi dengan vaksin influenza Flubio® yang berisi virus Influenza A/H3N2, A/H1N1 dan B/Washington. Pemurnian IgY hanya dilakukan pada kuning telur yang mengandung antibodi spesifik influenza. Deteksi keberadaan antibodi spesifik influenza ditentukan dengan uji agar gel presipitasi (AGP) dan metode enzyme linked immunosorbent assay (ELISA). Konsentrasi IgY hasil pemurnian dihitung dengan spektrofotometer dan karakterisasi protein dilakukan dengan metode Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Konsentrasi protein dari hasil pemurnian IgY memiliki nilai rata – rata 50,403 mg/mL. Hasil SDS-PAGE menunjukkan enam pita protein, yaitu struktur IgY utuh (174,63 kDa), Apoprotein-LDL (129,77 kDa), rantai berat IgY (66,54 kDa), Fab ( 47,65 kDa), rantai ringan IgY (21,85 kDa), dan β-livetin (36,74 kDa). IgY hasil pemurnian masih dapat berikatan dengan antigen influenza berdasarkan uji ELISA. Hasil penelitian ini menunjukkan bahwa pemurnian IgY menggunakan metode pengendapan NaCl dapat memurnikan IgY dengan struktur utuh, meskipun masih ada dua protein tidak spesifik hasil pemurnian. IgY yang dapat berikatan dengan antigen influenza mengindikasikan prospek pemanfaatannya untuk pencegahan influenza dan imunodiagnostik., Yolk immunoglobulin (IgY) contained in egg yolk has been widely used for immunotherapy and immunodiagnostics. One of the obstacles in IgY purification from egg yolk is separating Ig from fat, so that IgY purification techniques are always developing. This study aimed to purify IgY from egg yolk using sodium chloride (NaCl) precipitation method and to characterize influenza-specific IgY. Sodium chloride was chosen because this chemical is relatively unexpensive, environmentally friendly, and does not impair IgY immunoactivity. The egg yolks used in this study were collected from layer eggs vaccinated with the Flubio® influenza vaccine, which contains the Influenza A/H3N2, A/H1N1 and B/Washington viruses. IgY purification was only carried out on egg yolks containing influenza specific antibodies. The presence of influenza-specific antibodies was determined by the agar gel precipitation (AGP) test and the enzyme linked immunosorbent assay (ELISA) method. The purified IgY concentration was determined using spectrophotometer, and protein characterization was performed using the Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) method. Protein concentration from purification has a mean value of 50,403 mg/mL . SDS-PAGE result showed six protein bands, namely intact IgY structure (174,63 kDa), Apoprotein-LDL (129,77 kDa), IgY heavy chain (66,54 kDa), Fab (47,65 kDa), IgY light chain (21,85 kDa), and β-livetin (36,74 kDa). The purified IgY still able to bind with influenza antigen based on the ELISA test. The results of this study indicate that purification of IgY using the NaCl precipitation method can purify IgY with intact structure, although there are still two unspecific proteins in the purification results. The resulting IgY can bind to influenza antigens indicating the
Keyword: Immunoglobulin Y, influenza, egg yolk, purification, sodium chloride