Judul: Perincian Parameter Usability Tipe Pengguna Browser dan Evaluator pada Situs Web E-commerce Indonesia
Abstrak: The development of e-commerce is quite rapid. E-commerce is quite desirable for its ability to give service around the clock without geography boundaries. Website usability is one of supporting factor. This study gives evaluation for each usability parameter based on browser and evaluator user type. The Assesment criterion defined to each parameter before evaluation. Evaluation can be done by doing observation followed with giving score for each parameter. Parameter of browser user type consists of homepage, navigation, and search, while evaluator user type consists of Search Engine Result Page (SERP) and page of product detail. The Assesment criterion defined into 81 criterion for 38 parameter. The number of evaluated Indonesia e-commerce websites are 102 websites. Most of evaluated websites are 70.6% with a good final score.
Keyword: Bogor Agricultural University (IPB), websites, usability, parameter, e-commerce, evaluator, browser

Judul: Kajian Usability Website E-commerce Indonesia Berdasarkan Perspektif Tipe Pengguna Browser dan Evaluator
Abstrak: This research aims to measure usability of Indonesia e-commerce websites based on browser and evaluator user type in determining the readiness of Indonesia e-commerce. This research is performed by searching Indonesia e-commerce websites, preprocessing, determining parameters in accordance with the user type of browser and evaluator, evaluating the websites, and analyzing the results. Website evaluation results show that out of 144 websites, 66% and 64.65% meet the browser and evaluator user type respectively. In addition, based on the evaluation weighting, 80% of Indonesia ecommerce websites are at level 3, and 20% are at level 4 on the fulfillment of usability parameters. This indicates that the fullfillment of usability aspects of Indonesia e-commerce websites based on browser and evaluator user type is quite good.
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Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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