Judul: Pertumbuhan dan kelangsungan hidup benih ikan gurame yang diberi pakan alami yang disuplementasi hormon pertumbuhan rekombinan
Abstrak: The study was conducted to determine the effectiveness of feeding Artemia nauplii and silk worm supplemented with recombinant growth hormone (rHP) on giant gourami fry to increase its growth. This study consisted of four treatments with two replications. The treatments were (A) Artemia enriched with rHP and silk worm without rHP supplementation, (B) Artemia enriched with rHP and silk worms with not lisated bacteria producing rHP, (C) Artemia enriched with rHP and silk worm with lisated bacteria producing rHP, and ( D) control without rHP enrichment. Giant gourami larvae used was two-day-old after yolk egg absorbed and in amount of 50 fish for each replication. Artemia nauplii of 202 mg wet weight were immersed into 200 ml of rHP solution for 30 minutes, and then it divided equally into each aquarium. Feeding fish on rHP-enriched Artemia nauplii was once a day for one week of rearing. Two gram of silk worms were mixed with rHP and allowed for 15 minutes. Feeding fish on rHP-enriched silk was once a day for 2 weeks of rearing. Fish were raised for 8 weeks and fed on live food ad libithum. The results showed that absolute and specific growth rate of treatments and control was similar (P>0.05). Fish biomass in Treatment A (192.31 g) was relatively higher compared to other treatments (172.88 and 173.95 g) and control (170.50 g). Survival rate of fish in treatment A (99%) and control (100%) was higher (P<0.05) compared to treatment C (91%) and B (89%). Thus, Artemia nauplii could be used to deliver rHP in order to increase growth of giant gourami fry.
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Judul: Respons benih ikan gurame (Osphronemus goramy) yang diberi hormon pertumbuhan rekombinan melalui oral pada dosis berbeda
Abstrak: This study was aimed to evaluate the response of giant gourami juvenile on oral administration of recombinant giant grouper growth hormone (rElGH). A total of 45 juvenile were fed with rElGH coated by hydropropylmethyl cellulose phthalate (HP55) and mix with commercial diet (35% protein content). Feeding fish with diet containing four different doses of rElGH (0 mg/kg feed, 0.3 mg/kg feed, 3.0 mg/kg feed, and 30.0 mg/kg feed) was performed in twice a week for 2 month. Fish were then maintained for 1 month without rElGH treatment. The results showed that biomass harvest in treatment 3.0 mg/kg (1,376.0±38.3 g) and 30.0 mg/kg (1,362.2±78.5 g) was similar, and the two treatments were 46.76% and 44.28% higher compared to control (977.6±96.7 g), respectively. In addition, rElGH treatment increased appetite, while artificial feed conversion ratio (1.18) was lower (P<0.05) by 23.72% compared to that of control (1.46). Oral administration of rElGH also increased hepatosomatic index (16.12%) and protein content (17%), while lipid content (21%) decreased compared to control. Similar in fish survival at end of experiment, insulin-like growth factor-1 and growth hormone receptor-1 gene expression levels in brain at 24 hours after feeding in rElGH treatment and control were obtained. Thus, orally rElGH administration was effective to be applied to enhance the growth of giant gourami juvenile.
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Judul: Penentuan Nilai Eigen dan Vektor Eigen dari Matriks Tridiagonal 2-Toeplitz dengan Pendekatan Polinomial Chebyshev
Abstrak: The eigenvalues and eigenvectors of a matrix can be determined by finding its characteristic polynomials. The characteristic polynomials of a tridiagonal 2-Toeplitz matrix is shown to be closely connected to polynomials which satisfy the Chebyshev recurrence relationship. If the order of the matrix is odd, then the eigenvalues are found explicitly in terms of the Chebyshev zeros and the eigenvectors are found in terms of the polynomials satisfying the recurrence relationship. For even ordered matrices, the situation is more complicated. The problem in these cases is that although the Chebyshev recurrence formula is still applied, its initial values are not generating Chebyshev polynomials
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