Judul: Hubungan Kebiasaan Tidur dan Menonton Televisi dengan Status Gizi Remaja di SMP Bina Insani Bogor
Abstrak: In Indonesia, especially big cities, there has been lifestyle changing of adolescents who commonly tend to be sedentary physical activities. One of the sedentary physical activities that affect body mass index (BMI) is watching television. In addition, sleeping habits also influence adolescents BMI. The purpose of the present study was to determine the relationship between sleeping and watching television habits on adolescents and BMI. This study used a cross-sectional study design with purposive sampling. Samples consisted of 48 adolescents aged 13–15 years who were junior high school student at Bina Insani Bogor School. The results showed a significant relationship between adolescents BMI with gender (p = 0.01), father's education (p = 0.04), night sleep duration (p = 0.01) and the consumption of snacks while watching television (p = 0.03). However, there was no significant relationship between adolescents BMI with age (p = 0.26), maternal education (p = 0.05), level of physical activities (p = 0.77), night sleep time (p = 0.75), and duration of watching television (p = 0.93).
Keyword: watching television, sleeping, body mass index, adolescent

Judul: Kebiasaan Makan dan Perspsi Body Image pada Siswa smp Berstatus Gizi Lebih dan Normal
Abstrak: The purpose of this study was to analyze the differences between food habits and body image perception and the correlation between the two variables observed in overweight and normal nutritional status of junior high school boys students. This research used cross sectional design involved 100 students, 50 overweight and 50 normal status from two schools in Bogor. The data consist of individual and family characteristic, food habits, nutritional knowledge, body image perceptions, and healthy status. Most of the subject of 12 years old, with family members in the middle category, dan almost all parents are college graduates. Subjects have middle category of nutritional knowledge, and topic about healthy and safe food is answered correctly only by one third of the subjects.. The result found no difference in food habit (p>0.05), but significant difference (p=0.000) in body image perception. Was found no correlation (p>0.05) was between food habits and body image perception, but was found significant correlation exist (p=0.027) between nutritional knowledge and body image perception.
Keyword: boys., adolescent, body image, food habit

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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