Judul: Penapisan dan Identifikasi Bakteri Endofit dari Tanaman Binahong dan Ketepeng Cina Penghasil Senyawa Antibakteri Patogen
Abstrak: Endophytic bacteria is a beneficial microorganism that interacts with plant without causing any harm to the host. Most studies showed that certain endophytic bacteria can produce chemical compound which have medical effect for human, especially endophytic bacteria from medicinal plant. The objective of this research are to isolation endophytic bacteria from binahong and ketepeng cina that produce antibacterial compound and to identify the potencial isolate through 16S rRNA gene. Endophytic bacteria from both plants was tested to 5 human pathogenic bacteria, that are Escherichia coli dH5α, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, and Enterobacter sakazakii. Endophytic bacteria isolate from binahong code BB5 and BB14 have ability to inhibit 5 pathogenic bacteria growth (showed by inhibition zone). Identification based on 16S rRNA gene showed that both isolates possibly are Pseudomonas sp. KR 1.13.
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Judul: Identifikasi Cendawan Endofit Asal Tanaman Obat dan Penapisan Potensinya sebagai Antibakteri
Abstrak: Endophytic fungi are microorganisms living in plant tissue without causing any harm to its host. The fungi can be found in all plants including medicinal plants. Endophytic fungi produced variety of bioactive compounds such as antibacterial substances. This research aimed to identify 3 isolates of endophytic fungi JBa8, JMa3, and SLbt3 which were isolated from medicinal plants and to screen their potency as source of antibacterial compounds. Identification of endophytic fungi was performed based on morphological characteristics and DNA barcode using ITS region. Antibacterial test was conducted against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Enteropathogenic Escherichia coli (EPEC) K1-1, and microflora E. coli by antagonism technique and agar diffusion method using mycelia extract with methanol solvent. The inhibition activity of fungal extract on bacterial tested was observed on the day 3rd, 7th, 10th, and 14th after incubation by measuring clear zone around the colonies. The 3 isolates were tentatively identified as Fusarium solani for JBa8, Fusarium sp. for JMa3, and Colletotrichum sp. for SLbt3. Antibacterial activities were observed in F. solani and Fusarium sp. which were isolated from jati belanda (Guazuma ulmifolia) and jahe merah (Zingiber officinale), respectively. F. solani inhibited the growth of EPEC K1-1, whereas Fusarium sp. suppressed P. aeruginosa with clear zone produced was 12 mm. Fusarium sp. had antibacterial activity similar with that of 8 μg/mL cefotaxime positive control. The F. solani antibacterial activity, however, was lower than the two positive control concentrations used. Colletotrichum sp. isolated from sambiloto (Andrographis paniculata) did not have any antibacterial to all bacteria tested.
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Judul: Model Proyeksi Demografi Uniradix Multistate dan Aplikasinya Dalam Status Perkawinan
Abstrak: Demografi multistate merupakan pemodelan penduduk dengan transisi aolar slale, yaitu transisi aolar status sosia! yang dialami individu-indiviclu selring dengan perubahan \Vakiu. Pergerakan transisi ini dipengaruhi oleh faklar pendidikan. ekonomi. dan pemikahan. Transisi status sosial ini dapa! terjadi dalam satu wilayah, dua wilayah stau lebih
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