Judul: Perubahan Mutu Fisik Mentimun (Cucumis sativus L.) Pada Kemasan Plastik Polietilen dan Keranjang Bambu dalam Transportasi Darat
Abstrak: Transport model and selection on packaging type in land transportation of cucumber determine the level of mechanical damage which can degrade the quality of cucumber. Mechanical damage on cucumber grouped into wound scratch, wound rupture, bruising, and wound crack. Mechanical damage will affect the physical quality of cucumber. The objective of this research is to determine effect of some packaging types on cucumber physical quality change (polyethylene plastic and bamboo baskets). The observations were conducted based on the physical quality parameters such as mechanical damage, weight loss, color, hardness, and organoleptic tests. Simulations was performed using a simulator table for 2 hours and 3 hours with an amplitude of 2.5 cm and frequency of 2.75 Hz. Physical qualiy parameter of cucumber were observed before and after simulation. Polyethylene packaging showed better results in cucumber fruit packaging, with less mechanical damage (28.48% and 32.64%), weight loss (10:07% and 10.62%) and general acceptance of cucumber by the panelists after transport with a score of 3.5.
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Judul: Kajian Jenis Kemasan dan Simulasi Pengangkutan terhadap Mutu Fisik Buah Terung (Solanum melongena L.)
Abstrak: Eggplant is one of popular vegetable in Indonesia, commonly consumed in a fresh state or after cooking. The increase of demand of eggplanthas affected the need to improve the variety, quality, and the quantity of eggplant in the market. Proper postharvest handling is needed in order to mantain the quality of eggplant. The objective of this study is to find out the type of packaging and amount of mechanical damage along with the effect of the duration transportation simulation. The parameters observed in this research is based on the physical quality of eggplant such as mechanical damage, weightloss, hardness, total dissolved solids, and color change. The target of this study is a suggestion for type selection of packaging, that is ideal for the transportation of eggplant.
Keyword: transport simulation., physical quality, packaging, eggplant

Judul: Isolasi Konstruksi dan Rekombinasi Gen Pengkode Reverse Transcriptase asal Simian Retrovirus-2 (SRV-2)
Abstrak: The availability of reverse transcriptase enzym as a valuable component for Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique has been providing by foreign companies. Therefore, it needs more cost and time to provide it. This study is an early step to produce in house reverse transcriptase rekombinant enzym from SRV-2. The purpose of this study was isolated, constructed, and rekombined SRV-2 reverse transcriptase gene to entry clone and destination vector. Isolation was done by PCR amplification technique using three pairs of primers, which are RT I (3282-3683), RTII (3261-3900), and RTIII (3169-4133). Construction and recombination in this study used Gateway method from Invitrogen. SRV-2 reverse transcriptase gene was isolated in this study with A-549 cell inoculated SRV-2 as source of virus. This gene amplify by PCR technique and resulted specific band for RTI 401 bp, RTII 639 bp, and RTIII 964 bp. Analysis of pENTR/SD/TOPO and pDEST17 indicated SRV-2 reverse transcriptase gene code has already inserted to this plasmid. This result indicated SRV-2 reverse transcriptase gene have isolated, constructed, and recombined to entry clone and destination vector.
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