Judul: Sistem Pakar Fuzzy Penentuan Kesesuaian Jenis Ikan Berdasarkan Kualitas Air.
Abstrak: Pengetahuan tentang parameter kualitas air yang tepat adalah hal penting pada proses budidaya ikan karena optimalitas produksi ikan dipengaruhi oleh kualitas air. Penelitian ini bertujuan untuk membuat sistem pakar penentu jenis ikan budidaya berdasarkan kualitas air. Parameter kualitas air yang berupa parameter kimia adalah kandungan oksigen terlarut, amoniak, dan nitrit, sedangkan parameter fisika adalah suhu dan kecerahan. Ikan yang dipilih adalah ikan mas (Cyprinus carpio), gurame (Osphronemus goramy), dan patin (Pangasianodon hypophthalmus) masing-masing mewakili ikan dengan tolerasi rendah, sedang, dan tinggi terhadap kualitas air yang buruk. Hasil keakuratan sistem terhadap kualitas air parameter kimia sebesar 92% dan parameter fisika sebesar 85% untuk ikan mas, parameter kimia sebesar 63% dan parameter fisika sebesar 59% untuk ikan gurame, dan parameter kimia sebesar 89% parameter fisika sebesar 70% untuk ikan patin.
Keyword: expert system, fuzzy inference system, Mamdani

Judul: Sistem Pakar Penentuan Jenis Budidaya Ikan Air Tawar Berdasarkan Lokasi dan Kualitas Air
Abstrak: This research developed an expert system designed to record and use knowledge, experience, and expertise of the experts in aquaculture to identify what types of freshwater fish farming based on location and existing water quality. K-fold cross validation method is used for sharing subset of data train and data test with three replications for each training and testing. Nominal distance is used to calculate distances on the nominal parameters, while Euclidean distance is used to calculate numerical parameters that will be combined using aggregate formula. The resulting distances are combined by aggregate formula. The classification method used for determining the appropriate class type is K-Nearest Neighbor that give the best average of accuracy at k = 3 with normalization, with a value of 97.41%.
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Judul: Isolasi Konstruksi dan Rekombinasi Gen Pengkode Reverse Transcriptase asal Simian Retrovirus-2 (SRV-2)
Abstrak: The availability of reverse transcriptase enzym as a valuable component for Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique has been providing by foreign companies. Therefore, it needs more cost and time to provide it. This study is an early step to produce in house reverse transcriptase rekombinant enzym from SRV-2. The purpose of this study was isolated, constructed, and rekombined SRV-2 reverse transcriptase gene to entry clone and destination vector. Isolation was done by PCR amplification technique using three pairs of primers, which are RT I (3282-3683), RTII (3261-3900), and RTIII (3169-4133). Construction and recombination in this study used Gateway method from Invitrogen. SRV-2 reverse transcriptase gene was isolated in this study with A-549 cell inoculated SRV-2 as source of virus. This gene amplify by PCR technique and resulted specific band for RTI 401 bp, RTII 639 bp, and RTIII 964 bp. Analysis of pENTR/SD/TOPO and pDEST17 indicated SRV-2 reverse transcriptase gene code has already inserted to this plasmid. This result indicated SRV-2 reverse transcriptase gene have isolated, constructed, and recombined to entry clone and destination vector.
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