Judul: Glucose biosensor using a glucose dehydrogenase flavin adenine dinucleotide electrochemically immobilized on zeolite nanoparticles
Abstrak: A glucose biosensor was developed to measure blood glucose levels. The performance of these new glucose biosensors was continuously improved, producing better performance and stability. The technique selection and immobilization matrix are key factors for biosensor development, so that the prospecting of materials that can be used as a matrix for supporting enzyme, continue to be explore. The purpose of this study was to determine the activity and stability of the enzyme glucose dehydrogenase (GDH) that were immobilized on zeolite nanoparticles (NPZ) and determine the kinetic parameters and analytical performance by electrochemical methods. The resulting enzyme electrode with NPZ and enzyme electrode with zeolite A showed a high activity against the enzyme electrode glucose than without NPZ. The reaction kinetics of third electrodes of enzyme followed the Dixon equation. The 𝐼����𝑚����𝑎����𝑥����𝑎����𝑝����𝑝���� values for enzyme electrode without NPZ, with NPZ, and with zeolite A were 27.25 μA, 77.52 μA, and 48.54 μA, respectively. The 𝐾����𝑀����𝑎����𝑝����𝑝���� values for GDH enzyme that immobilized on the NPZ were 1.16 mM, 12.30 mM for GDH enzyme that immobilized on zeolite A and 0.95 mM for GDH enzyme immobilization without NPZ. The enzyme electrode without NPZ, with NPZ, and with zeolite A also showed a high sensitivity with value for the sensitivity were 0.58 μA mM-1, 0.81 μA mM-1, and 2.51 μA mM-1, respectively. The third enzyme electrodes showed different linear area with range for the enzyme electrode without NPZ, with NPZ and with zeolite A were 1.00-15.00 mM, 2.00-20.00 mM, and 0.06-10.00 mM, respectively. The blood glucose level of normal person ranged from 4 to 6 mM, so that the enzyme electrode with NPZ and without NPZ are suitable for use in the determination of the concentration of blood glucose. While the enzyme electrode with zeolite A has a smaller range so it is more suitable to use in the determination of glucose concentration in the foods or beverages. The enzyme electrode with NPZ and the enzyme electrode with zeolite A showed a good accuracy with relative error values were 3.84% and3.48%, respectively. Repeatability measurements also showed a good precission results with relative standard deviation values (%RSD) for the enzyme electrode with NPZ and enzyme electrode with zeolite A were 3.83% and 3.16%, respectively. By combining two immobilization techniques, physical adsorption on zeolite and entrapment on carbon paste, high enzymatic activity, good accuracy, and repeatability was attained. The enzyme electrode with NPZ and the enzyme electrode with zeolite A demonstrated a good electrochemical response toward glucose, allowing it to detect blood glucose levels and glucose levels in foods and beverages.
Keyword: glucose biosensor, immobilization, zeolite

Judul: Stabilitas dan Efektivitas Biosensor Glukosa Menggunakan Escherichia coli Yang Diimobilisasi Pada Matriks Nanokomposit Zeolit
Abstrak: Biosensor combines the selectivity of biological recognition element and the sensitivity of the transducer. The application of biosensor as blood glucose measurer for diabetes mellitus patients uses purified enzyme as biological sensor. The substitution of purified enzyme with the microbial cells producing enzymes as biological sensor is carried out to overcome the weaknesses of purified enzyme such as the low stability of the enzyme activity and the high cost. This research was carried out to measure the activity of glucose dehydrogenase (GDH)–the enzyme reacting specifically with glucose as substrate and binding with both pyrolloquinoline quinone (PQQ) and Mg2+ ion as prosthetic group and activator respectively-which is exhibited by escherichia coli, using electrochemical methods Design of experiments only to optimalization the activity of GDH bacterial cells of e. coli is a method of surface response. Optimum conditions of activity of GDH derived cells of e. coli bacteria in the absence of a matrix of nanocomposite zeolite on the process of immobilization is the temperature of 30°C, pH 6, concentration of glucose 15 mM, the concentration of PQQ 3,050 μM. As for the optimum conditions for the activity of GDH bacterial cells of e. coli with matrix nanocomposite zeolite is the temperature of 30°C, pH 6, glucose concentration of 15 mM, the concentration of PQQ 3,050 μM, and nanocomposite zeolite 137,50 mg. The reaction catalyzed by GDH kinetics of Glucose Kinetics equation follows the Lineweaver-Burk. The value of KM app bacterial cells of e. coli with the larger of nanocomposite zeolite mM compared to 1,4580 without nanocomposite of zeolite matrix 0,720 mM. This shows that affinity GDH e. coli immobilization of glucose substrate without nanocomposite zeolite is greater compared to the matrix nanocomposite zeolite.GDH activity stability of bacterial cells is not much different compared with no matrix nanocomposite zeolite.
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Judul: Warna bulu kucing ras Persia : variasi pola warna dan pewarisannya
Abstrak: Kucing Persia merupakan kucing ras yang paling umum dijumpai di Indonesia. Kucing Persia memiliki bulu yang panjang dengan variasi warna bulu yang sangat beragam. Perkawinan yang lebih terarah sangat perlu dilakukan untuk meningkatkan kualitas genetik warna dan pola warna bulu kucing. Penelitian ini bertujuan untuk mempelajari variasi warna dan pola warna bulu kucing Persia yang terdapat di Indonesia, yang dilakukan di kantor pusat Cat Fancy Indonesia mulai bulan Oktober 2000 sampai Januari 2001. Data yang digunakan merupakan data pedigree kucing Persia yang tercatat di Cat Fancy Indonesia dari tahun 1997 sampai Oktober 2001 yang terdiri dari 187 jantan dan 258 betina, selain itu juga dilakukan pengamatan langsung kepada tiga penyayang dan breeder kucing yang ada di Bandung. Secara umum tercatat 104 penyayang dan breeder kucing ras di Indonesia, 74,04% terdapat di Jawa Barat dan sisanya (25%) terdapat di daerah Jakarta. Jumlah kucing Persia yang lahir di Indonesia adalah 387 kucing (87,16%), yang terdiri dari 163 (42,12%) jantan dan 224 (57,88%) betina. Jumlah kucing Persia yang diimpor ke Indonesia adalah sebanyak 57 (12,845%) yang terdiri dari 23 (40,35%) jantan dan 34 (59,65%) betina. Jumlah kucing Persia yang terdapat di Jawa Barat (84,14%) lebih banyak dibanding jumlah kucing Persia yang ada di Jakarta (14,86%). Secara umum warna bulu cream (9,01%) dan pola warna solid (34,01%) memiliki persentase tertinggi. Warna cream merupakan warna dilusi dari warna merah (red) yang dapat dihasilkan dari berbagai macam kombinasi warna bulu yang dimiliki tetua sebelumnya, sebagai contoh dari perkawinan tetua yang memiliki warna bulu cream dengan red smoke. Perkawinan antara tetua yang memiliki warna dan pola warna bulu yang tidak murni dapat menghasilkan turunan yang memiliki warna dan pola warna bulu yang bervariasi.
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