Judul: Comparison of Modular Channels with Canvas Concrete for the Planning of Tertiary Irrigation Channel in Argopeni Village Kebumen
Abstrak: Saluran irigasi tersier Desa Argopeni melayani lahan seluas 98.65 ha. Kondisi saluran saat ini menggunakan konstruksi urugan tanah yang menyebabkan kehilangan air yang besar. Ketika musim hujan debit aliran yang besar menyebabkan dinding tererosi sehingga mengakibatkan pendangkalan saluran. Kondisi ini mengurangi efisiensi irigasi. Oleh karena itu, diperlukan upaya untuk menyelesaikan masalah tersebut. Saat ini inovasi irigasi telah ditemukan, sehingga penelitian dilakukan untuk merencanakan saluran tersier dengan menerapkan teknologi irigasi berupa saluran modular dan beton kanvas kemudian membandingkan keduanya dari aspek desain, rencana anggaran biaya, dan efektivitas pekerjaan. Penelitian ini dilakukan di Saluran Tersier Desa Argopeni, Kebumen dengan panjang panjang 448 m. Untuk menentukan dimensi saluran terlebih dahulu menghitung kebutuhan air di area layanan. Hasil penelitian menunjukkan bahwa diperlukan 2.80 liter/detik/ha untuk memenuhi kebutuhan air pada petak tersier. Efisiensi saluran tersier sebesar 80% sehingga kapasitas saluran adalah 0.47 m3/s. Saluran direncanakan berbentuk trapesium dengan kemiringan dinding 1:1.5; lebar dasar 0.8 m; lebar atas 1.2 m; tinggi muka air 0.60 m; dan tinggi jagaan 0.2 m, total tinggi saluran adalah 0.80 m. Saluran modular terdapat modul yang memiliki ketebalan 0,8 m dengan konfigurasi 1-0, artinya terdiri dari modul 1 lantai, 2 modul siku, dan 2 modul dinding dengan anggaran yang direncanakan sebesar Rp 260.501.000,00,-. Saluran beton kanvas menggunakan tipe 8 (CC8) dengan ketebalan 0.8 m dan lebar gulungan 1.1 m dengan anggaran Rp 707.980.000,00,-. Pekerjaan saluran ini direncanakan pada bulan Oktober dengan jenis saluran terpilih yaitu saluran modular karena memiliki efektivitas pekerjaan yang lebih baik dibandingkan saluran beton kanvas menurut kebutuhan biaya dan efektivitas pekerjaan.
Keyword: beton kanvas, kebutuhan air irigasi, saluran irigasi tersier, saluran modular

Judul: Perencanaan dan Perancangan Saluran Irigasi Terbuka di Balai Besar Penelitian Tanaman Padi (Subang, Jawa Barat).
Abstrak: Irrigation techniques provides water with the right conditions, the right space, and timely in a way that is effective, economical, and efficient. Planning and design of irrigation system refers to the contour of the land and the flow pattern of the system is needed. So it will impact in increase of productivity and quality of the harvest quality. Placement of irrigation channel adjoining the location of the farm, intended to have a higher elevation with farmland. The planning of secondary irrigation channels along the branches 4196.47 m with tertiary channels have an average 400-800 m length. With an area of 343.89 ha of agricultural land in total consisting of zone 1, 2, and three. Irrigation water requirements by using an open channel with trapezoidal cross-section and use the design method of Strickler required discharge of 0.6043 m3 / s with a coefficient of crop water 1,46 l / s / ha. The results of channel design successfully done according to the appendix 3, in cross section two secondary channels are used to accommodate the irrigation discharge of 0.6043 m3 / s, k = 35, m = 1, n = 2, b = 1,142 m, y = 0,571 m, v = 0,6 m / s, and I = 0,0012. Based on the results of modeling using HEC-RAS software 4.1 on irrigation channels can be said to be safe, and able to accommodate discharge of irrigation according to the needs of 0.6043 m3 / sec with input value n (manning roughness) of 0.025 and a efficiency of secondary channel 90% and 95% of tertiary channels.
Keyword: design, planning, irrigation, HEC-RAS

Judul: Using cytochrome β (Cyt β) gene for species identification of dog, cat, and tiger to ensure food authenticity and drugs
Abstrak: Adulteration food products from animal still happen until now. Species identification method development in food and medicine derived from animal was needed to prevent falsification information. Molecular genetic improvement allows the detection is carried out at the level of DNA, so the results more accurate, although processed. Research on several meat have been conducted by several researchers using DNA mitochondrial DNA (mtDNA). Using mtDNA because it has high copy number in a cell, compared with nuclear DNA. In this case where the amount of extracted DNA is very small or degraded. Gene used as marker specific species is cytochrome β (cyt β) because it has varied sequences. The objective of this research was to study species authentication (i.e. dog, cat, and tiger) to ensure animal origin in product using cyt β gene specific marker. Total DNA samples for each species which identified are three samples were consisting blood (cat), cooked meat (dog), feces (tiger), and as a comparison used blood sample from several animal (i.e. goat, chicken, cattle, sheep, horse, rat) and pork. Reverse primer specific for dog, cat, and tiger was designed using MEGA 5 software. DNA extraction and fragment amplification was conducted using phenol-chloroform and multiplex PCR method, respectively. Multiplex PCR is a variant of PCR which many primers were used together for amplification of multiple region. This research showed that different length of amplification for the species tested (dog, cat, and tiger) were 523, 331, 319 bp, respectively; and for the comparison species (goat, chicken, cattle, sheep, pig, horse, and rat) were 157, 227, 274, 331, 398, 439, and 603 bp, respectively. Species specificity also indicated by high reverse primer homology percentage. Specific fragment amplification on genome pool with closed fragment length should be separate to avoid band overlapped between species. Taxonomy hierarchy (i.e. dog, cat, and tiger) could be distinguished to the species level.
Keyword: cat, cytochrome β gene, dog, multiplex PCR, tiger