Judul: Efisiensi Hara pada Rumput Golf dengan Pemberian Pupuk Hayati
Abstrak: objective of the research was to study the effect of using biofertilizer on nutrient efficiency of turf grass. The research was conducted at the Rainbow Hills Golf Course, Cijayanti, Bogor on February to May 2012. The experiment was designed at randomized complete block design with two factors and three replications. The first factor is inorganic fertilizer with four dosages (A1:100%; A2:75%; A3:50%; and A4:0% of inorganic fertilizer recommended dosage (DA)) as main plot and the second factor is biofertilizer with four dosages (H1:100%; H2:75%; H3:50%; and H4:0% of biofertilizer recommended dosage (DH)) as sub plot. The results showed that biofertilizer aplication increased coverage area, density, crown dry weight, shoot-root ratio, clipping dry weight, nutrient efficiency (uptake and use), and reduced inorganic fertilizer
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Judul: Pengaruh dosis pupuk hayati mikoriza terhadap serapan hara, pertumbuhan dan kualitas turgrass
Abstrak: Penelitian bertujuan untuk mendapatkan tingkat dosis pupuk hayati mikoriza arbuskula yang optimum pada turfgrass var Tifdwarf (Cynodon dactylon x Cynodon transvaalensis). Percobaan terdiri atas satu faktor yaitu dosis mikoriza yang terdiri atas beberapa taraf. Penelitian dilakukan dengan menggunakan rancangan acak kelompok (RAK). Dosis pupuk hayati mikoriza yang digunakan terdiri dari atas 0 g/pot (M0), 100 g/pot (M1), 200 g/pot (M2), 300 g/pot (M3), 400 g/pot (M4), 500 g/pot (M5). Percobaan dilakukan dengan 4 ulangan sehingga terdapat 24 satuan percobaan. Pengamatan dilakukan terhadap kecepatan penutupan, pertambahan tinggi vertikal, lebar daun, densitas, warna, panjang akar, bobot kering hasil pangkas, persentase infeksi mikoriza pada akar, jumlah spora, kandungan klorofil a dan b tajuk, dan kandungan hara N dan P tajuk. Pemberian pupuk hayati mikoriza meningkatkan jumlah spora, persentase infeksi akar, dan persentase penutupan rumput. Pemberian pupuk hayati mikoriza mengurangi pertambahan tinggi vertikal rumput dibanding dengan perlakuan tanpa pupuk hayati mikoriza. Pemberian pupuk hayati mikoriza tidak meningkatkan kandungan dan serapan hara N dan P tajuk. Namun, kandungan hara N dan P tajuk telah menunjukkan kandungan hara yang optimum untuk rumput tifdwarf. Pemberian dosis pupuk hayati 300 g/pot pada penelitian ini merupakan perlakuan terbaik karena mampu mencapai persentase penutupan rumput, pertambahan tinggi vertikal, bobot kering hasil pangkas, densitas, tekstur, jumlah spora, persen infeksi pada akar, serapan dan kandungan hara yang optimum pada turfgrass.
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Judul: Mitochondrial DNA Gene Organization and Phylogenetic Relationship of Naja siamensis
Abstrak: Naja siamensis is one of Asiatic cobra in the Family of Elapidae, known as a cryptic snake. It has many morphological variations that lead to misidentification. Misidentification can cause the wrong concept in developing antivenom types due to high snakebite cases occur in Thailand. Thus, an accurate species identification using a molecular approach is crucially required. Mitochondrial DNA has been used widely in snake molecular studies. These studies were aimed to determine the arrangement of the N. siamensis partial mitochondrial genes using reptile-oriented primers and to reveal the phylogenetic relationship among other species of Asian cobra. DNA extraction was carried out based on the standard DNA salting-out protocol and was conducted by bioinformatics analysis using BLASTn and MEGA 6 software. Gene annotation was performed against N. kaouthia complete genome. A total of 3121 bp of N. siamensis mtDNA genes were sequenced consisting of partial of six regions: 16S rRNA, ND1, tRNA-Ile, CRII region, tRNA-Leu, tRNA-Gln, and COI genes. The mtDNA gene organization of N. siamensis was predicted to has a similar pattern with the common Elapidae mtDNA gene organization. This study showed that there is a monophyletic form in Naja genus and N. kaouthia is the closest species with N. siamensis based on partial 16S rRNA and ND1 gene.
Keyword: 16S rRNA, Elapidae, ND1, phylogenetic relationship