Judul: Kualitas Tidur Karyawan di Jakarta
Abstrak: DKI Jakarta is a capital city of indonesia and it has became a megapolitan city. DKI Jakarta as a central of business and government also, so its citizens and workers have high activities. High activity is so frequently associated with sleep quality. Sleep is a condition when the brain does not process the information from sensory neurons and does not give the command to motor neurons. The aim of this research were to collect the data and to determine sleep quality of employees in Jakarta. In determining sleep quality, 295 subjects were measured with Pittsburgh Sleep Quality Index (PSQI). Present study shows that sleep quality of older employees better than younger. Statistical analysis indicates that there is significant negative correlation between age and sleep quality (p=0.0322). Either male or female employees have similar percentages between their good and poor sleep quality. Thus, sex does not significantly affect to sleep quality and they have no statistical correlation (p=0.1615). Income and education indirectly are not much important factor in relation to sleep quality. Statistical analysis shows that sleep quality has no statistical correlation to income (p=0.5432) and education (p=0.6621).
Keyword: Age, education, employees, income, sex, sleep quality.

Judul: Studi Kualitas Tidur Pegawai Institut Pertanian Bogor
Abstrak: Sleep is an important activity undertaken by humans and spend nearly a third of the human life time. Sleep is a condition in which the brain does not process information from sensory neurons and does not give orders to the motor neurons. Sleep quality of person can be determined using the Pittsburgh Sleep Quality Index (PSQI). The data of this research were obtained by giving questionnaires contain personal data and questions about the components of sleep quality during the past month There are seven components of sleep quality, subjective sleep quality, sleep latency, sleep duration, sleep efficiency, sleep disturbances, use of sleeping medication, and daytime dysfunction. The purpose of this study was to determine the sleep quality of employee of Bogor Agricultural University. Value of PSQI obtained from the calculation of the seven components, if someone has a value of PSQI < 5 then he has good sleep quality, and if someone has value of PSQI >5 then he has poor sleep quality. The purpose of this study is to determine the sleep quality of employee of Bogor Agricultural University. The proportion of male employee who had good quality sleep were equal to the proportion of female who had good quality sleep. As much as 48.27% of the total sample of employees have good sleep quality. As much as 62,5% of employees over the age of 51 years have poor sleep quality.
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Judul: Delesi Sinyal Sekresi Gen CalBsyn dalam Plasmid pGAPZα-proCalBsyn-egfp melalui Teknik Mutagenesis Terarah.
Abstrak: ProCalBsyn merupakan gen penyandi Candida antartica lipase B yang dibuat secara sintetik dalam plasmid pGAPZα-proCalBsyn-egfp (4839 bp). Sekuen proCalBsyn masih mengandung sinyal sekresi native (ss-CalBsyn), sementara plasmid pGAPZα memiliki sinyal sekresi mating-factor-α.(MFα). Tujuan dari penelitian ini adalah mendelesi sinyal sekresi ss-CalBsyn, mengintroduksi situs EcoRI serta transformasi plasmid mutan ke E.coli TOP10F’. Delesi ss-CalB dilakukan dengan teknik mutagenesis terarah melalui proses PCR. Sepasang primer F-mCalB dan R-mCalB serta high-fidelity DNA-polymerase G-Hifi digunakan untuk amplifikasi. Hasil amplifikasi PCR diperoleh plasmid mutan berupa DNA linear berukuran 4780 bp. Setelah direstriksi dengan enzim EcoRI dan ligasi, plasmid mutan ditransformasikan kembali ke E.coli. Transformasi menghasilkan lebih dari 60 koloni transforman. Analisis PCR menunjukkan 3 dari 9 klon hasil isolasi plasmid yang dianalisis terindikasi membawa plasmid mutan yaitu klon 7, 19 dan 20. Ketiga klon tersebut menunjukkan adanya pita gen mCalBsyn (970 bp).
Keyword: mutagenesis terarah, mutasi delesi, sinyal sekresi native CalB, proCalBsyn, plasmid pGAPZα