Judul: Isolasi dan Kloning Gen Sitokinin Oksidase (OsCKX) Pengendali Sifat Produktivitas dari Padi Varietas Ciherang
Abstrak: Gn1a/OsCKX gene (grain number 1a/Cytokinin Oxidase) of rice (Oryza sativa L.) that encodes cytokinin oxidase has been identified as a quantitative trait loci that contribute to an increase in the number of grains of rice. This study aimed to isolate an OsCKX gene from rice cv. Ciherang. Amplification of OsCKX gene was done by PCR (Polymerase Chain Reaction) technique using specific primer pairs and cDNA template of rice cv. Ciherang. Subsequently, OsCKX gene fragment was cloned into a cloning vector pGEM-T Easy using T4-ligase enzyme and transformed into competent cell Escherichia coli Dh5α through heat shock method, and grown on LB agar medium containing ampicillin. Result showed that amplification with specific primers of OsCKX gene generated an amplicon fragment with size of ± 2000 bp. The gene fragment was succesfully cloned into pGEM-T vector and transformed into cells of E. coli Dh5α. Verification of recombinant clone by cutting using two restriction enzymes SmaI and KpnI regenerated two bands of 3015 bp (pGEM-T Easy vector) and 2000 bp (OsCKX gene fragment).
Keyword: rice (Oryza sativa L.), restriction enzymes, pGEM-T Easy vector, OsCKX gene, gene isolation, Cloning

Judul: Karakterisasi Molekuler Padi Transgenik dengan Beberapa Metode Isolasi DNA
Abstrak: Rice is a major food commodity for agroindustrial country. Grain size is a major determinant of grain quality. One attempt that has been made in research to increase rice production is assembly of transgenic plants inserted with Os-GS3 (Oryza sativa Grain Size 3) gene. The material used in this study is cultivar Taipei 309. Rice plants that have been inserted with Os-GS3 gene were grown and the presence of the genes was analyzed through molecular characterization by PCR and Southern hybridization. DNA isolation methods used in this study were the simple method, miniprep scale, and large scale. They were compared to obtain the most efficient method in terms of turnaround time and resources used for PCR and Southern hybridization. PCR analysis of the three methods produced bands of the same size, in that 500 bp. Southern hybridization test of the DNA isolated using the large scale method produced bands or copy genes, where as that isolated using miniprep method did not produce band. The fast scale method is the most efficient DNA isolation method for PCR analysis, where as the large scale method is the most efficient DNA isolation method for Southern hybridization. The results in the silencing of gene expression of Os-GS3 with antisense RNA was successful when 4 transgenes integrated into the plant genome.
Keyword: 

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
Keyword: