Judul: Optimasi Metode Loop-mediated Isothermal Amplifcation (LAMP) untuk Uji Kehalalan Daging Segar.
Abstrak: Metode loop-mediated isothermal amplification (LAMP) merupakan alternatif dari metode PCR dengan tingkat sensitifitas yang sama namun menggunakan peralatan yang lebih sederhana. Hal ini penting untuk infrastruktur pengujian halal di berbagai daerah yang mungkin memiliki keterbatasan sumber daya pengujian, khususnya dalam menyongsong kewajiban sertifikasi halal. Penelitian ini bertujuan memperoleh kondisi optimal pengujian LAMP sebagai metode deteksi babi yang sensitif dan sederhana untuk uji kehalalan pangan. Gen sitokrom B dan gen troponin I babi diamplifikasi mengunakan komposisi reagen, proses amplifikasi serta metode deteksi yang bervariasi. Hasil pengujian menunjukkan bahwa reaksi dapat berlangsung dengan tambahan 2 primer loop (loopF dan loopB) dan optimum pada suhu 65 oC selama 45 menit, dilanjutkan suhu 80 oC selama 5 menit. Kedua gen target dapat diamplifikasi dan tidak bereaksi silang dengan spesies lain. Sejauh ini, hanya elektroforesis yang memberikan hasil berbeda antara kontrol positif dan negatif. Meskipun menunjukkan hasil positif, reaksi LAMP memerlukan optimasi lebih lanjut sehingga lebih sensitif dan DNA hasil amplifikasi mampu divisualisasi dengan metode yang lebih sederhana.
Keyword: babi, DNA, halal, LAMP, sitokrom B, troponin I

Judul: In Silico LAMP Primer Design for Pig Gene Detection Applications in Processed Meat Products
Abstrak: Kasus pencemaran daging babi pada produk olahan daging meningkatkan isu pentingnya identifikasi kehalalan pangan. Salah satu metode identifikasi yang dapat digunakan yaitu loop-mediated isothermal amplification (LAMP). Tujuan penelitian ini adalah mendesain primer LAMP menggunakan DNA mitokondria babi pada gen 12S rRNA, 16S rRNA, dan sitokrom b secara in silico. Metode yang digunakan adalah preparasi sekuen, penentuan dan identifikasi daerah target desain primer, desain primer menggunakan Primer Explorer V5, identifikasi primer, dan uji spesifitas primer secara in silico. Hasil penelitian diperoleh set primer gen sitokrom b dengan urutan basa F3: CCACCCCATATTAAACCAGAA, B3: GGTTGTCCTCCA-ATTCATGTT, F2: CTACGCTATCTACGTTCAATTCC, F1c: AGGATGG-AGGCTACTAGGGCTAAC, B2: AGGTCTGCTACTAGTATTCAGA, B1c: TGCCCATACTACACACATCCAAACA, LB: ATTTCGACCACTAAGTC-AATGCCT memenuhi parameter ideal dan memiliki spesifitas terbaik sehingga dapat digunakan untuk uji lanjutan secara in vitro menggunakan metode LAMP., The cases of pork contamination in processed meat products raises the issue of the importance of identifying halal food. One of the identification methods that can be used is loop-mediated isothermal amplification (LAMP). The purpose of this study was to design a LAMP primer using pig mitochondrial DNA in the 12S rRNA, 16S rRNA, and cytochrome b genes in silico. The research steps consisted of sequence preparation, determination and identification of target region primer design, primer design using Primer Explorer V5, identification of primer and in silico primer specificity test. The results of the study obtained a primary set of cytochrome b genes with the order of bases F3 CCACCATATTAAACCAGAA, B3: GGTTGTCCTCCA-ATTCATGTT, F2: CTACGCTATCTACGTTCAATTCC, F1c: AGGATG-GAGGCTACTAGGGCTAAC, B2: AGGTCTGCTACTAGTATTCAGA, B1c: TGCCCATACTACACACATCCAAACA, LB: ATTTCGACCACTAA-GTCAATGCCT meets ideal parameters and has the best specificity so it can be used for advanced testing in vitro using the LAMP method.
Keyword: 12S rRNA, 16S rRNA, cytochrome b, LAMP, processed meat  products

Judul: Thermal Characteristics and Dynamics of Tropical Cyclone over Western North Pacific Ocean: A Case Study of Typhoon Songda (Chedeng) 2011
Abstrak: The frequent occasion of Tropical Cyclone (TC) over Western North Pacific (WNP) Ocean lies from 10˚N to 26˚N and 121˚E to 170˚E. Sources of TC development come from warm ocean temperature where latent heat is released during condensation processes and subsequently concentrated in the boundary layer. Relative humidity at 850-700 hPa is responsible to produce the most intense storm. Meanwhile, weak vertical wind shear between 850-200 hPa is one of the dynamical factors that lead to the development of TC. Pressure level of 850-200 hPa is chosen to minimize the thermodynamic effect in the lower troposphere. Typhoon Songda (Chedeng in the Philippines) or TSC lasted from May 18th to 30th, 2011 and strengthened into powerful Super Typhoon stage between May 24th and 27th. Long curving track and the exhibition of all sequences of TC life cycle, TSC is then considered as an ideal case of TC. The objective of the study with case of TSC is to investigate the role of thermal characteristics and dynamics on TC intensity. The research is carried out by using Advanced Research of Weather Research and Forecasting model (WRF ARW model) version 3.3. Two domains are employed in the model. The first domain situate from 02˚N to 40˚N and 118˚E to 149˚E and cover spatial resolution () of 33.1 km. The second domain situate from 09˚N to 27˚N and 119˚E to 132˚E with of 11 km. Timestep of the model () is 120 seconds. The model is supported by the following physical parameterization, i.e., Yonsei University Scheme (YSU) in the boundary layer, Kain-Fritsch scheme (KF) in the cumulus, and WRF Single-Moment 3-class scheme in the microphysics. Initial meteorological condition prior to the formation of the cyclone is generated using WRF ARW standard-release model. Sensitivity of the model output is obtained from a selected single-parameter change scenarios, namely, 10 % to 20 % decrease and increase of wind shear initial data at 850-200 hPa, and by 10 % to 20 % decrease of relative humidity initial data at 850-700 hPa. The result showed that the negative correlations between wind shear and intensity are found in wind shear scenarios. Wind shears of 10 ms-1 which lead to strengthening of the wind intensity in the early period of Typhoon development are found in wind shear scenarios. The effect of wind shear changes on wind intensity is statistically significant in the 20 % wind shear scenarios. During mature stage, weak wind intensity occurred when wind shear increase as given in the WRF standard-release and the 20 % decreasing scenario. Different situation is found in the 20 % increasing scenario where wind shear tend to weak and lead to strong wind intensity at this stage. Meanwhile, the reduction of moisture supply at 850-700 hPa resulting from 20 % decreasing relative humidity play a major role in the weakening of wind intensity and reduces mechanical energy of the cycle process as much as 300 J kg-1.
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