Judul: The hypoglycemic activity 0f protein hydrolysate, concentrate, and isolate of sea cucumber (Holothuria scabra J.) in rats
Abstrak: Sea cucumber (Holothuriascabra J.) contains high protein. The protein consists of several specific amino acids that can lower blood glucose, making it beneficial for patients with diabetes melitus (DM). The research aims to study the influence of free amino acids content in protein hydrolysate, concentrate, and isolate of sea cucumber on the reduction of blood glucose in rats. The study consisted of five stages: (1) preparation and proximate analysis of sea cucumber meat, (2) analysis of total and free amino acids hydrolysate, concentrate, and isolate, (3) determination of the hypoglycemic effect in rats, (4) bioassay (in vivo), and (5) testing of histopathology (hematoxylin-eosin and imunohistochemical). The results showed that protein content of sea cucumbers of meat and powder were 72.93%db and 72.07%db, respectively. Hydrolysate, concentrate, and isolate showed relativelely similar content of total amino acid at level, 48.6%, 47.77%, and 48.61% respectively. However, the free amino acid content of hydrolysate was higher (2.88 %) than those of free amino acid content of concentrate (0.25%) and isolate (0.22%). The treatment of 300 mg/kg (dw) of hydrolysate, concentrate, and isolate showed the best hypoglicemic effect in rats. In vivo test showed that treatment of DM+hydrolysate, DM+concentrate, and DM+isolate lower blood glucose levels and promote weight loss. These result were better than treatment of positive control (DM). Decreasing of blood glucose levels was noticed at about 53.9%, 6.6%, and 27.6% respectively, whereas weight gain was noticed at about 22.6%, 12.9%, and 17.3% respectively. The result intracellular antioxidant enzyme activity showed that hydrolisate, concentrate, and isolate was quite capable of maintaining the activity of the enzyme superoxide dismutase (SOD) and was unable to maintain the activity of the enzyme glutathione peroxidase (GPx) and catalase. Histopathologic test with hematoxylin-eosin staining showed that the number of islets per field of view in group DM+hydrolysate treated rats was greater than those found in the DM+concentrate and DM+isolate groups. The number of islet were 2.47±0.92, 1.80±0.78, and 2.00±0.93 respectively. The number of islets of Langerhans found in positive and negative control rats were 1.80±0.78 and 3.07±0.80 respectively. Imunohistochemical test showed that the number of beta cells in the DM+hydrolysate group was higher than those of the DM+concentrate and DM+isolate groups, namely 40.10±6:35, 37.42±27.61, and 28.40±10.75. The number of beta cells in negative and positive control groups were 84.30±20.02 and 12.50 ±3.21 respectively.
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Judul: Efek Pemberian Teripang Pasir (Holothuria scabra J) terhadap Profil Imunohistokimia Antioksidan Dismutase (SOD) pada Pankreas Tikus Diabetes
Abstrak: The aim of this research is to analyse the effect of sea cucumber (Holothuria scabra J) on the profile of antioxidant enzymes copper,zinc-superoxide dismutase (Cu,Zn-SOD) in the pancreatic of diabetes mellitus rats. A total of 25 male white rats (Sprague Dawley) were used for this study. They were divided into five groups; (1) negative control (KN), (2) positive control, rats with diabetes mellitus (KP), (3) diabetic rats treated with sea cucumber protein hydrolyzate (HDL), (4) diabetic rats treated with sea cucumber protein concentrated (KST), (5) diabetic rats treated with sea cucumber protein isolate (ISL). Diabetic condition conducted by alloxan injection with doses 110 mg/kg bb. Pancreatic β cells will be damaged by alloxan, then the insulin secretion will decrease. The treatment were done for 28 days. The pancreatic tissue were obtained after treatment ends. Pancreatic tissue were fixed with Bouin solution and then processed into histological preparations. The tissue were then stained with immunohistochemical staining techniques using monoclonal antibody of Cu,Zn-SOD. The results showed that treatment of HDL, KST, and ISL sea cucumber (Holothuria scabra J) increased content antioxidant Cu,Zn-SOD in pancreatic tissue. The HDL treatment gave the best effect in increasing the antioxidant content of Cu,Zn-SOD in pancreatic tissue of diabetic rats.
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Judul: Konsep Lanskap Kampung Tradisional Sillanan Berbasis Budaya Setempat
Abstrak: Konsep Lanskap Kampung Tradisional Sillanan Berbasis Budaya Setempat. Dibimbing oleh ANDI GUNAWAN. Kampung Sillanan memiliki konsep lanskap permukiman yang terdiri dari ruang permukiman lama dan permukiman baru. Penelitian ini bertujuan untuk mengidentifikasi karakter lanskap meliputi elemen lanskap, tata letak elemen, serta faktor pembentuk permukiman tradisional dari sudut pandang budaya setempat hingga menyusun konsep lanskap permukiman tradisional Kampung Adat Sillanan. Penelitian ini berlokasi di Kampung Adat Sillanan yang terletak di Kecamatan Mengkendek, Kabupaten Tana Toraja. Penelitian ini menggunakan metode analisis deskriftif melalui penelurusan informasi budaya dan dilakukan observasi lapang serta wawancara tokoh adat. Kampung Adat Sillanan kuat dengan aturan adat Aluk To Dolo. Permukiman tradisional Sillanan merupakan permukiman dengan desain cluster yang bersifat berkumpul dan dipisahkan oleh jalan lingkungan. Setiap cluster terdiri dari rumah, Tongkonan, dan alang (lumbung) yang masing-masing menghadap kearah utara. Permukiman tradisional Sillanan memiliki pola permukiman yang penetapan polanya berdasarkan aturan kosmologi, pada bagian datar yang bersifat mengelompok dan bagian berbukit berpenduduk jarang. Konsep lanskap permukiman yang meliputi elemen hardscape dan softscape serta tata letak dan filosofi menurut masyarakat setempat.
Keyword: landscape design, residential landscape, vernacular landscape, tongkonan, toraja