Judul: Pengaruh Dukungan Orang Tua dan Status Identitas Ego terhadap Kematangan Karier Mahasiswa Tingkat Akhir
Abstrak: Mahasiswa tingkat akhir perlu memiliki kematangan karier untuk memasuki dunia kerja setelah lulus dari Perguruan Tinggi. Penelitian ini bertujuan menganalisis pengaruh karakteristik mahasiswa tingkat akhir, karakteristik keluarga, dukungan orang tua, dan status identitas ego terhadap kematangan karier. Sebanyak 100 mahasiswa tingkat akhir yang dilibatkan dalam penelitian ini, terdiri atas 48 persen laki-laki dan 52 persen perempuan mahasiswa tingkat akhir yang dipilih dengan teknik proportional random sampling. Data dikumpulkan dengan teknik self report, kemudian data dianalisis menggunakan analisis deskriptif dan inferensia. Hasil penelitian menunjukkan kematangan karier mahasiswa tingkat akhir antara laki-laki dan perempuan tidak berbeda signifikan. Dukungan ayah secara otonomi dan status identitas ego achievement berpengaruh positif signifikan terhadap kematangan karier, sebaliknya status identitas ego diffusion dan status identitas ego moratorium berpengaruh negatif terhadap kematangan karier.
Keyword: dukungan orang tua, kematangan karier, kesiapan karier, pemuda, status identitas ego

Judul: Pengaruh Peran Pengasuhan Ayah terhadap Kekuatan Karakter dan Prestasi Akademik Mahasiswa
Abstrak: Peran pengasuhan ayah sangat penting dalam pembentukan kekuatan karakter dan prestasi akademik mahasiswa. Penelitian ini bertujuan untuk menganalisis pengaruh karakteristik mahasiswa, karakteristik keluarga, dan peran pengasuhan ayah terhadap kekuatan karakter dan pengaruh kekuatan karakter terhadap prestasi akademik. Penelitian ini menggunakan desain eksplanatori dengan melibatkan 289 mahasiswa tingkat pertama yang memiliki orang tua lengkap dan tinggal di asrama. Penarikan contoh diambil secara purposive sampling. Data diperoleh dengan pelaporan diri melalui kuesioner daring serta dianalisis dengan uji regresi linear berganda dan regresi logistik. Peran pengasuhan ayah dipersepsikan oleh sebagian besar mahasiswa pada kategori rendah. Rata-rata kekuatan karakter mahasiswa berada pada kategori tinggi dan hampir separuh mahasiswa memiliki indeks prestasi yang termasuk kategori baik. Hasil uji regresi linear berganda (R2=0.106) menunjukkan bahwa peran pengasuhan ayah berpengaruh terhadap kekuatan karakter dan hasil uji regresi logistik menunjukkan bahwa kekuatan karakter berpengaruh terhadap prestasi akademik mahasiswa.
Keyword: indeks prestasi kumulatif, kekuatan karakter, mahasiswa tingkat pertama, pengasuhan ayah, prestasi akademik

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable