Judul: Flushing pada Tanaman Amherstia nobilis dan Brownea capitella di Kebun Raya Bogor
Abstrak: Phenology is a study on periodicity sightings of plants in relation to climate, such as flowering time and flushing. Flushing means the growth of shoots/ flush simultaneously. This study aims to observe the shoots and leaves growth in response to light intensity under the difference of growing positions and environmental factors. This study used purposive sampling as a descriptive method. The growing positions of flush were on terminal and axillary of the plant, both in exposed and shaded conditions. The growth pace speeds of Amherstia nobilis and Brownea capitella in exposed condition were faster than shaded condition. The rate of growth was accompanied by a change of color. The changing color of A. nobilis leaves were faster for two months than B. capitella. This study showed that light intensity and growing positions of the plants (terminal and axillary) influenced the growth of both species.
Keyword: Phenology, Flushing, Flush, Amherstia nobilis, Brownea capitella

Judul: Studi Periode Flushing Pohon dan Aplikasinya dalam Taman.
Abstrak: Pohon merupakan salah satu vegetasi yang dapat menjadi daya tarik suatu lanskap. Salah satu penelitian yang dapat memberikan informasi tentang jenis pohon yang tepat sesuai dengan fungsi serta nilai estetikanya adalah studi fenologi. Fenologi adalah ilmu tentang periode fase-fase yang terjadi secara alami pada tumbuhan yang dipengaruhi oleh keadaan lingkungan sekitar, seperti lama penyinaran, suhu, dan kelembaban udara. Salah satu peristiwa fenologi yang paling mudah diamati adalah flushing pohon. Flushing adalah pertumbuhan tunas yang terjadi secara bersamaan pada waktu tertentu. Lokasi penelitian ini berada di Institut Pertanian Bogor, Kampus Dramaga. Penelitian ini dilakukan selama 6 bulan dimulai dari bulan Maret sampai Agustus 2018. Penelitian ini bertujuan mengetahui periode flushing pohon, mengetahui pengaruh faktor iklim terhadap persentase flushing pohon, dan menyusun rekomendasi rancangan penanaman menggunakan pohon yang diamati. Pohon tersebut, yaitu Bouea macrophylla Griff., Brownea coccinea Jacq., Maniltoa grandiflora Scheff., Maniltoa spp., dan Saraca thaipingensis Cantley ex Prain. Sampel pohon yang diamati sebanyak 5 spesies dengan 3 pengulangan pada masing-masing jenis pohon. Analisis data terdiri dari analisis warna daun menggunakan software Munsell Conversion, analisis kadar klorofil daun menggunakan spektrofotometer, dan analisis pengaruh faktor iklim terhadap persentase flushing pohon menggunakan metode grid dan analisis regresi linear. Berdasarkan hasil pengamatan, persentase flushing Bouea macrophylla Griff. berkisar 0.10 – 7.46 %, Brownea coccinea Jacq. berkisar 0.55 – 1.25 %, Maniltoa grandiflora Scheff. berkisar 1.19 – 5.34 %, Maniltoa spp. berkisar 0.11 – 5.98 %, dan Saraca thaipingensis Cantley ex Prain. berkisar 0.58 – 4.04 %. Semua jenis pohon mengalami periode flushing yang cenderung sama, tetapi puncak flushing setiap jenis pohon berbeda. Sebagian besar pohon mengalami puncak flushing pada bulan Maret dan Juli. Hasil analisis regresi linier berganda menunjukkan bahwa jenis pohon Brownea coccinea Jacq. dipengaruhi secara nyata oleh kombinasi unsur iklim suhu rata-rata dengan kelembaban udara rata-rata dan penyinaran, kelembaban udara rata-rata dengan lama penyinaran, dan curah hujan dengan lama penyinaran. Pohon Saraca thaipingensis Cantley ex Prain. juga dipengaruhi secara nyata oleh kombinasi unsur iklim suhu rata-rata dengan kelembaban udara, curah hujan, dan lama penyinaran, kelembaban udara rata-rata dengan curah hujan, dan curah hujan dengan lama penyinaran. Tiga jenis pohon lainnya tidak dipengaruhi secara nyata oleh semua kombinasi unsur iklim yang ada. Selain itu, penelitian ini juga membuat rancangan penanaman pohon di area Gedung Fakultas Ekonomi dan Manajemen (FEM) menggunakan pohon yang telah diamati. Penataan pohon yang dibuat akan memberikan kesan semarak, meningkatkan estetika, dan menciptakan suasana yang tidak membosankan atau monoton untuk dapat mendukung kegiatan belajar dan mengajar di kampus.
Keyword: Desain penanaman, fenologi, iklim, periode flushing

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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