Judul: Histopathological Changes of The Liver, Kidney, and Spleen in mice (Mus musculus) after Human-Platelet Rich Plasma Injection
Abstrak: Terapi sel berbasis sel manusia di Indonesia memiliki potensi yang tinggi untuk memenuhi kebutuhan unmet medical need. Sel berbasis manusia hanya bisa diujicobakan kepada manusia apabila mampu melewati uji praklinik, dimana sediaan yang diuji harus aman bagi hewan coba. Tujuan penelitian ini yaitu mengetahui pengaruh Human-Platelet Rich plasma terhadap jaringan mencit melalui analisa histopatologi organ hati, ginjal, dan limpa. Sepuluh ekor mencit jantan strain DDY berumur 8 minggu dibagi ke dalam 2 kelompok. Sebanyak 5 ekor mencit diinjeksi dengan human-PRP dengan dosis 4.1 × 10& sel/ gram BB, dan 5 ekor mencit diinjeksi dengan normal saline. Mencit dipelihara selama 14 hari, kemudian dinekropsi dan diambil organ hati, ginjal, dan limpa. Organ dibuat menjadi preparat histopatologi dengan pewarnaan HE. Hasil penelitian yaitu PRP menyebabkan jumlah sel Kupffer menjadi lebih sedikit, hepatosit tidak mengalami piknosis dan sitoplasma menjadi lebih basa, penyempitan ruang bowman ginjal, dan limpa didominasi oleh splenosit immature. Penyuntikan human-PRP tidak menimbulkan efek toksik bagi organ hati, ginjal, dan limpa., Human based cell therapy has a high potential to comply the unmet medical need in Indonesia. Human based cell can only be tested on human only if it has passed the preclinical trial, where the substance is proven to be safe for the animal model. This study aims to find out the effects of human-platelet rich plasma on mice tissue through the analysis of liver, kidney, and spleen histopathology. Ten male DDY strain mice aged 8 weeks were divided into two equal groups. Five mice were injected with human-PRP with 4.1 × 10& cells/ gram weight dose, while the other five were injected with normal saline. Mice were necropsied on the 14th day with the liver, kidney, and spleen collected. The organs were made into histopathology slide and stained with HE under the same condition. The results are reduced numbers of Kupffer cells, non-pyknotic nuclei and alkalized cytoplasm of hepatocyte, narrowed Bowman’s space, and domination of immature splenocytes. These results shows that human-PRP injection had no toxic effects on liver, kidney, and spleen.
Keyword: cell therapy, histopathology, platelet rich plasma

Judul: Quantitative Analysis of Histopathology Changes in Liver, Kidney, and Spleen Tissue of Mice (Mus musculus) after Injection of Human Mesenchymal Stem Cells (hMSCs) and Conditioned Medium (CM)
Abstrak: Terapi stem cell menjadi salah satu metode pengobatan baru yang paling menjanjikan untuk mengobati penyakit degeneratif dan iskemik. Human Mesenchymal Stem Cells (hMSCs) dan Conditioned Medium (CM) dapat menghasilkan respon imun. Penelitian ini bertujuan untuk mempelajari respon jaringan hati, ginjal, dan limpa mencit (Mus musculus) melalui gambaran histopatologi setelah diberi hMSCs dan CM melalui vena coccygea. Mencit DDY jantan dengan jumlah 15 ekor dibagi menjadi tiga kelompok perlakuan, kelompok hMSCs, kelompok CM, dan kelompok kontrol yang diinjeksi normal saline. Mencit dinekropsi pada hari ke-14 dan diambil sampel organ untuk difiksasi dengan formalin 10%, kemudian dibuat preparat histopatologi dengan pewarnaan Haematoxylin Eosin (HE) dengan processing yang sama sehingga warna comparable. Gambar histopatologi di analisa secara kuantatif menggunakan aplikasi ImageJ ver. 1.50g dan R software. Hasil penelitian menunjukkan jumlah sel Kupffer menurun yang mengindikasikan tidak adanya inflamasi, hepatosit diduga mengalami regenerasi, luas ruang Bowman kelompok CM menyempit, sel tubulus kelompok hMSCs lebih mature, dan splenosit kelompok hMSCs dan CM immature. Perubahan tidak memperlihatkan adanya respon toksisitas pada jaringan hati, ginjal, dan limpa mencit., Stem cell therapy is one of the most promising new treatment methods for treating degenerative and ischemic diseases. Human Mesenchymal Stem Cells (hMSCs) and Conditioned Medium (CM) can produce an immune response. This study aims to determine the response of liver, kidney, and spleen tissue of mice (Mus musculus) through histopathology after being given hMSCs and CM through the coccygeal vein. The 15 male DDY mice were divided into three treatment groups, hMSCs, CM, and control injected with normal saline. Mice were necropsied on the 14th day and organ samples were taken to be fixed with 10% formalin, then histopathology preparations were made with Hematoxylin Eosin (HE) staining with the same processing so that the color was comparable. Histopathology images were analyzed quantitatively using the ImageJ app version 1.50 g and R software. The result showed that the number of Kupffer cells decreased which indicated the absence of inflammation, hepatocytes are thought to regenerate, Bowman’s space tightened in the CM group, tubular cells in the hMSCs group were more mature, and splenocytes in the hMSCs and CM groups were immature. The changes did not show any toxic response in the liver, kidney, and spleen tissues of mice.
Keyword: conditioned medium, human mesenchymal stem cells, kidney histopathology, liver histopathology, spleen histopathology

Judul: Construction of Strongly Optimal Binary Linear Code with Minimum Distance 9 and 11
Abstrak: A binary linear code of length n over q F is a subspace of n q F . A code has three parameters that attached to it, namely length, dimension, and minimum distance. A code with length n, dimension k and minimum distance d is often called [n, k, d ]-code. Usually, when two parameters are given, then we want to find a code that has the best value for the last parameter. Based on Gilbert- Varshamov bound, if a [n, k, d ]-code exists and can not be expanded, we call it a strongly optimal code. In this paper, we construct strongly optimal code with minimum distance 9 and 11. In constructing the code, we created a theorem and algorithm based on Gilbert-Varshamov bound before we implement the algorithm to MAPLE programming language. Because of computational limitations, the program can only construct up to k = 10 for d = 9 and k = 12 for d = 11.
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