Judul: Luasan Optimal Hutan Kota sebagai Penyerap CO2 dan Kesesuaiannya dengan Rencana Tata Ruang Wilayah di Kota Tangerang.
Abstrak: Tangerang City known as industrial area and residential area. The city has consumed a lot of energy, that lead to the increase of air pollution especially CO2 in atmosphere. One way to reduce level of air pollution is development of green open space, urban forest, and protected area within spatial planning of Tangerang City. The necessary of urban forest in the Tangerang City could be determined by CO2 absorption approach based on IPCC (Intergovermental Panel on Climate Change) method. The research aimed at estimating the optimal size of urban forest based on CO2 emission came from domestic, agriculture and industrial emission. Further, spatial suitability also were examined. Result showed that CO2 emissions amounted to 3188.9 Gg CO2/years in 2014. Meanwhile, the result of land cover classification revealed that the area of trees vegetation amounted to 1870.26 hectares and grass amounted to 1977.81 hectares. Both of trees vegetation and grass could only absorb 115.48 Gg CO2/years (3.62% of total emissions). The protected area established in land use plannning only 1421.06 Ha and therefore need a lot of additional urban forest, in fact there was no any urban forest established by the government.
Keyword: emission, spatial planning, urban forest

Judul: Penentuan Luasan Optimal Hutan Kota sebagai Penyerap Karbondioksida di Kabupaten Tegal Jawa Tengah
Abstrak: Tegal Regency is a city that is experiencing rapid development. This development makes things such as people’s activities and transportation increases. It tends to ignore the presence of lands that have vegetation. It makes carbondioxide (CO2) gas emission from transportation and people increases. Urban forest concept is one of effective and efficient way to improve life quality in urban areas through its ability to absorb the CO2 from the air. This research was to explore optimal area of urban forest based on CO2 emissions that are generated by oil and gas fuel and humans. This research was done by field observation and literature studies. CO2 emissions in Tegal on 2011 amounted to 66 879 965.11 ton, so urban forest area that needed tends to 1 148 004.12 ha. Tegal Regency now have 1.03 ha of urban forest area, so it needs to be enhanced. CO2 emissions increases each year. It will reach to 80 427 512.27 ton on 2025, so it requires 1380549.70 ha of urban forest area on that year.
Keyword: urban forest, Tegal, emissions, carbon dioxide

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
Keyword: