Judul: Genetic diversity analisis on pineapple (Ananas comosus (L.) Merr.) by random amplified polymrphic dna (RAPD)
Abstrak: Pineapple is belong to the Bromeliaceae, a large family of American tropics (only one species originated from Africa) which has 45 genera and 2 000 species. Out of six species of pineapple, Ananas comosus (L.) Merr. is the only species grown commercially for its fruit (Nakasone and Paull, 1998). To date, world cultivation just based on six cultivars, i.e., Cayenne, Queen, Singapore Spanish, Red Spanish, Abacaxi and Perolera, while the first three have been the genetic base of distribution from the original area. In Indonesia, cultivars that recommended by Department of Agriculture are only Queen (Bogor pineapple and Palembang pineapple) for fresh fruit and Cayenne (Smooth or Lisse) for processing uses and assumed to have low genetic variability. In this study, 17 accessions of pineapple (Ananas comosus (L.) Merr.) were evaluated for their morphological performance and subjected to analysis of Random Amplified Polymorphic DNA (RAPD). Eight morphological characters of the accessions were measured to examine their morphological relationship. For RAPD analysis, thirteen primers of 10-mers were used to amplify the genomic DNA of the pineapple accessions. Out of them, three random primers yielded monomorphic products while the remaining amplified a total of 20 (87%) fragments, all of which were polymorphic, with an average of 1.77 fragments per primer. A dendogram based on UPGMA-link method and principal component analysis (PCA) plots were examined the clustering pattern and distribution of the accessions, both for morphological characters and amplification products. As the result, RAPD analysis showed higher coefficient of similarity between pineapple accessions tested rather than morphological's, which may indicate the high environmental influence on the morphological performance. This study was demonstrated that RAPD analysis was able to detect genetic similarity among accessions in one species, eventhough among all primers used, there was no single primer could distinguish between Queen and Smooth Cayenne group of pineapple tested. ...
Keyword: 

Judul: Keragaman Genetik Nenas (Ananas comosus (L.) Merr.) berdasarkan Penanda Morfologi dan Amplified Fragment Length Polymorphism (AFLP)
Abstrak: Information on genetic diversity is essential in breeding program. Characterization of germplasm for providing genotype variation data can be accomplished by phenotypic or genotypic assessment. The aim of this research was to know the genetic diversity among 21 pineapple accessions from Sumatera, Kalimamtan, Sulawesi, Irian Jaya, Jawa Barat, Bali and Jepang based on morphological character and Amplified Fragment Length Polymorphism (AFLP). The morphological data consisted of length, width, spine position and coloration of leaves. The data were categorized into class data and converted to binary form. Two selected primers P11 and M48 were used to generat AFLP markers. The twenty nine AFLP fragments were scored as binary form based on the presence (1) and absence (0) of each fragment. Similarity matrix was obtained to perform cluster analysis based on Dice coefficient. Dendrogram was constructed through clustering analysis using SPSS-13 software. An integrated similarity analysis of morphological and AFLP data showed that similarity coefficient among the 21 accessions ranged between 0.67-1.00. All the 21 accessions were clustered into tree groups at similarity index of 0.74. The first group consisted of 17 accessions, the second group consisted of 3 accessions and the third group contained 1 accession. However, all 21 accessions were closely related each other at 0.67 similarity coefficient.
Keyword: 

Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4