Judul: Kualitas lingkungan pengasuhan, kelekatan ibu-anak, dan perkembangan kognitif anak usia prasekolah pada keluarga dengan ibu bekerja
Abstrak: Penelitian ini bertujuan untuk menganalisis kualitas lingkungan pengasuhan, kelekatan, perkembangan kognitif anak usia prasekolah, dan korelasi antar variabel penelitian. Penelitian dilakukan di Kecamatan Tanah Sareal dan Kecamatan Bogor Barat, Kota Bogor dan melibatkan 39 anak usia prasekolah beserta ibunya yang dipilih secara purposive. Pengambilan data dilakukan dengan teknik wawancara dan observasi dengan alat bantu kuisioner dan Alat Permainan Edukatif (APE). Data dianalisis dengan analisis deskriptif dan korelasi. Hasil penelitian menunjukkan bahwa anak memiliki kategori sedang pada kualitas lingkungan pengasuhan, kelekatan, serta perkembangan kognitif. Lama pendidikan ibu berhubungan positif signifikan dengan kelekatan dan kulitas lingkungan pengasuhan. Sementara itu perkembangan kognitif tidak berkorelasi dengan variabel apapun….
Keyword: Home learning environment, attachment, cognitive development, working mother, preschool child, Human ecology, Cross sectional study, Grand parenting, Spearman

Judul: The Influence of Family Welfare, the Quality of Home Environment, and the Quality of Preschool Education on Children’s Cognitive Development
Abstrak: Penelitian ini bertujuan untuk menganalisis: (1) perbedaan antara kesejahteraan keluarga, kualitas lingkungan pengasuhan, kualitas pendidikan prasekolah, dan perkembangan kognitif anak yang mengikuti pendidikan prasekolah formal dan nonformal, (2) pengaruh karakteristik keluarga terhadap kesejahteraan keluarga, (3) pengaruh karakteristik anak, karakteristik keluarga, dan kesejahteraan keluarga terhadap kualitas lingkungan pengasuhan, dan (4) pengaruh karakteristik anak, karakteristik keluarga, kualitas lingkungan pengasuhan, dan kualitas pendidikan prasekolah terhadap perkembangan kognitif anak., This study aimed to analyze: (1) the difference between family welfare, quality of home environment, quality of preschool education, and cognitive development of children who are attending formal and nonformal preschool education, (2) the influence of family characteristics on family welfare, (3) the influence of child characteristics, family characteristics, and family welfare on quality of home environment, and (4) the influence of child characteristic, family characteristics, quality of home environment, and quality of preschool education on child's cognitive development.
Keyword: cognitive development, family welfare, home environment, preschool education

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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