Judul: Partisipasi Masyarakat dalam Kegiatan Pembangunan Desa Melalui Strategi Gerakan Desa Makmur Merata (Gersamata) di Propinsi Sulawesi Tenggara
Abstrak: Penelitian lapang bersifat studi kasus yang di lakukan di desa Sindang Kasih, yang terdapat di Kecamatan Ranomeeto, Kabupaten kendari, Sulawesi Tenggara. Pemilihan lokasi didasarkan pada pertimbangan bahwa lokasi tersebut merupakan salah satu lokasi sub pengembangan kegiatan dengan pendekatan dan strategi GERSAMATA, disamping pertimbangan jarak dan transportasi yang dapat di jangkau. Responden di pilih secara acak sebanyak 40 orang yang tersebar di ketiga dusun desa Sindang Kasih, dengan sistem les.
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Judul: Peranserta Masyarakat dalam Pembangunan Desa (Studi Kasus di Desa Malasari, Kecamatan Nanggung, Kabupaten Bogor, Jawa Barat)
Abstrak: PEran serta masyarakat dalam pembangunan diperlakukan untuk mempercepat terwujudnya tujuan pembangunana. Motivasi dan latar belakang masyarakat berpengaruh terhadap peranserta mereka dalam pembangunan. Kenyataan tersebut merupakan ruang lingkup permasalahan yang telah dilapang. Selain itu dilihat juga kesesuaian program pembangunan pemerintah atas desa dan keinginan masyrakat. Praktek Lapangan dilaksanakan di Desa Malasari, Kecamatan Nanggung, Kabupaten Bogor selama tiga bulan. Metoda yang digunakan adalah wawancara dan pengamatan langsung terhadap kegiatan pembangunan yang sedang berlangsung.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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