Judul: Application High Resolution Melting (HRM)-qPCR Method for Identification of Silky Shark in Fisheries Products
Abstrak: Penggunaan ikan hiu sebagai bahan baku produk olahan perikanan di Indonesia mengancam eksistensi hiu salah satunya spesies hiu lanjaman (C. falciformis). Metode deteksi cepat berbasis molekuler dengan sensitivitas tinggi dan terjangkau diperlukan untuk identifikasi ikan pada produk perikanan. Aplikasi metode HRM-qPCR dapat digunakan sebagai solusi. Penelitian ini bertujuan mendapatkan primer spesies spesifik secara in silico untuk identifikasi spesies C. falciformis dari berbagai lokasi pendaratan ikan. Prosedur penelitian meliputi desain primer, isolasi DNA sampel, pengujian kualitas dan kuantitas DNA, dan optimalisasi HRM-qPCR. Desain primer menggunakan aplikasi Mega-X dan Bioedit pada marka gen mtDNA berhasil dilakukan. Isolat DNA dan primer NADH2 digunakan dalam analisis HRM-qPCR. Spesies target dan non target dapat dibedakan dengan sebaran garis pada kurva leleh dan nilai Tm. Nilai Tm Sampel C. falciformis dari daerah Aceh sebesar 72,85-73,2°C, sedangkan sampel dari NTB berkisar 73,15-73,3°C. Primer-primer dengan marka gen selain NADH2 pada penelitian ini dapat digunakan dalam penelitian selanjutnya. Pengujian menggunakan pewarna Sensi-fast HRM dari Bioline memiliki nilai Tm yang lebih tinggi dengan tingkat kenaikkan suhu kecil sehingga hasilnya lebih baik daripada menggunakan pewarna Evagreen dari Biotium., The use of sharks as raw material for processed fisheries products in Indonesia threatens the existence of sharks, one of them is silky shark (C. falciformis). Molecular-based identification methods with high sensitivity and reliability are needed for fish identification in fishery products. The application of the HRM-qPCR method can be used as a solution. The aim of this research was to design in silico species-specific primers for identification of C. falciformis from various fish landing sites. The research procedures included designing specific primers, isolation of DNA samples, testing the quality and quantity of DNA samples, and optimization of HRM-qPCR. The primary design using Mega-X and Bioedit applications on mtDNA gene markers was successfully made. DNA isolates and NADH2 primers were used in HRM-qPCR analysis. The target and non target species can be distinguished by the distribution of lines on the melting curve and the Tm value. The Tm value of C. falciformis from Aceh was 72,85-73,2°C, while the samples from NTB was 73,15-73,3°C. The other primers with gene markers than NADH2 in this study can be used in future studies. The tests using sensi-fast HRM dye from Bioline has a higher Tm value with small temperature increase so that the results are better than using Evagreen dye from Biotium.
Keyword: Hiu, Primer spesifik, C. falciformis, HRM, Suhu

Judul: Autentikasi Ikan Hiu Dilindungi pada Produk Perikanan dengan Metode qPCR dan Marka Gen NADH Dehydrogenase subunit 2
Abstrak: Hiu merupakan salah satu hasil perikanan yang memiliki nilai ekonomis tinggi. Perdagangan hiu umumnya berupa potongan tubuh, seperti sirip, daging, kulit, dan ekor sehingga dilakukan identifikasi secara molekuler sebagai upaya mengetahui produk hiu yang diperdagangkan. Tujuan penelitian ini mendesain primer spesifik berbasis gen marka NADH2 pada ikan hiu dilindungi (Rhincodon typus, Sphyrna lewini, S. mokarran, S. zygaena, Carcharhinus longimanus, C. falciformis, Alopias pelagicus, A. pelagicus, dan Rhynchobatus australiae) serta aplikasinya dalam autentikasi produk perikanan berbasis qPCR. Tahapan penelitian terdiri dari perancangan primer spesifik, isolasi DNA, pengujian kuantitas isolat DNA, dan identifikasi menggunakan qPCR. Hasil perancangan primer pada sembilan spesies hiu dilindungi berhasil dilakukan. Primer Alopias pelagicus NADH2 dan Carcharhinus falciformis NADH2 hasil rancangan dapat mengamplifikasi DNA target., Sharks are one of the fishery products that have high economic value. Shark trade is generally in the form of body parts, such as fins, meat, skin, and tails so that molecular identification is carried out to identify the shark products being traded. The purpose of this study was to design specific primes based on the NADH2 marker gene. The primer will be used to identify protected sharks species (Rhincodon typus, Sphyrna lewini, S. mokarran, S. zygaena, Carcharhinus longimanus, C. falciformis, Alopias pelagicus, A. pelagicus, and Rhynchobatus australiae) and their application in qPCR-based authentication of fishery products. The research stages consisted of specific primer design, DNA isolation, testing the quantity of DNA isolate, and identification using qPCR. The results of the primer design on nine protected shark species were successfully implemented. Alopias pelagicus NADH2 and Carcharhinus falciformis NADH2 primers were designed to amplify target DNA.
Keyword: Shark, NADH2, primer, qPCR

Judul: Pelabelan Super Edge Magic pada Graf Cycle dan Graf Wheel
Abstrak: This manuscript proves that cycle graph and wheel graph have a super edge magic labeling. Super edge magic labeling on a graph is labeling that has an edge magic labeling with a set of vertices were mapped in to {1,2,… ,𝑝} and a set of edges were mapped in to {𝑝+1,𝑝+2,… ,𝑝+𝑞}, in which 𝑝 is order and 𝑞 is size on the graph. There are one lemma and two theorems to be discussed. The lemma is used to prove the two theorems. The first theorem proves that cycle graph 𝐶𝑛 is super edge magic if and only if 𝑛 is odd. The second theorem proves that wheel graph 𝑊𝑛 of order 𝑛 is not super edge magic. Moreover 𝑊𝑛 is not edge magic if 𝑛≡0 mod
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