Judul: Analisis faktor-faktor yang memengaruhi keputusan pembelian di Maraca Books and Coffee
Abstrak: Meningkatnya jumlah pengunjung kafe di Indonesia menunjukan bahwa tingkat gaya hidup yang semakin meningkat sedangkan tingkat ketertarikan membaca buku masyarakat Indonesia masih sangat rendah. Hal ini membuat para pelaku bisnis kafe untuk mengembangkan inovasi library café yaitu kafe yang menyediakan fasilitas membaca berbagai jenis buku. Tujuan dari penelitian ini adalah (1) untuk mengetahui karakteristik konsumen, (2) proses pengambilan keputusan konsumen dan (3) faktor-faktor yang memengaruhi keputusan pembelian konsumen di Maraca Books and Coffee. Responden pada penelitian ini yaitu 100 orang. Metode yang digunakan adalah non probability sampling dengan teknik purposive sampling. Penelitian ini menggunakan bantuan program Statistical Package for the Social Sciences (SPSS) versi 23 untuk menguji validitas, reliabilitas, analisis deskriptif dan analisis faktor. Hasil penelitian terdapat delapan faktor yang berpengaruh terhadap keputusan pembelian konsumen yaitu ketertarikan buku dan kenyamanan membaca, kualitas produk kopi dan kemudahan lokasi, kualitas pelayanan, daya tarik pribadi dan promosi, ketertarikan coffee, ramah lingkungan, fasilitas pendukung dan pengaruh kerabat.
Keyword: analisis faktor, keputusan pembelian, Maraca Books and Coffee

Judul: Faktor-Faktor Yang Mempengaruhi Keputusan Pembelian Minuman Kopi Di Malabar Mountain Cafe Kota Bogor
Abstrak: Penelitian ini bertujuan untuk menganalisis bagaimana proses keputusan pembelian serta faktor-faktor yang mempengaruhi keputusan pembelian kopi di Malabar Mountain Cafe. Penelitian ini bersifat studi kasus dengan teknik pengambilan sampling menggunakan metode convenience sampling dan jumlah sampel yang digunakan sebanyak 80 orang. Metode yang digunakan dalam analisis ini yaitu menggunakan analisis deskriptif dan analisis faktor. Analisis deskriptif digunakan untuk mengidentifikasi karakteristik konsumen serta bagaimana proses pengambilan keputusan pembelian konsumen Malabar Mountain Cafe, sedangkan analisis faktor digunakan untuk mengelompokkan variabel-variabel yang memiliki kemiripan untuk dijadikan satu faktor sehingga akan diperoleh faktor-faktor utama yang membentuk keputusan pembelian. Berdasarkan hasil perhitungan analisis faktor, diperoleh tujuh faktor utama yang membentuk keputusan pembelian yaitu 1) harga dan karakteristik produk (8.252), 2) pelayanan dan fasilitas (2.639), 3) promosi dan tempat (1.744), 4) manfaat (1.631), 5) kepribadian (1.356), 6) pengaruh lingkungan (1.259), dan 7) kualitas (1.142).
Keyword: Bogor, faktor, kopi, konsumen, pembelian

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable