Judul: Pola asuh makan, Perkembangan Bahasa dan Kognitif pada Anak Balita Stunted dan Normal di Kelurahan Sumur Batu Bantar Gebang Bekasi
Abstrak: The problem of protein energy inadequacy is an major problem among underfives that effects of child growth. The prevelance of stunted and severe stunted status among underfives in Indonesia in 2010 based on Height/Age (H/A) Index was 35.6 % (18.5% severe stunted and 17.1% stunted). The purpose of this study to identify the association between nutrition status and language and cognitive development of underfives also differences of language and cognitive development between stunted and normal childern. The study design was a cross-sectional study. Sample size was 70 children consisted of 35 normal and 35 stunted childern. The highest percentage level of education of fathers and mothers only primary school and more than half of children came from poor families. It was show that stunted childern had lower language and cognitive development than normal childern. There was significant difference (p<0.05) of language and cognitive development between normal (non-stunted) childern and stunted. Also there was significant difference (p<0.05) between age of childern, mother level of education and family income with language and cognitive development. Child care practices was no difference between stunted childeren and non-stunted childern. There was a significant association between nutrition status based on Height/Age with language development (p<0.05), but it was no association with cognitive developmentm (p>0.05).
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Judul: Praktek Pemberian Makan, Konsumsi Pangan, Stimulasi Psikososial dan Perkembangan Balita Stunting dan Normal
Abstrak: Stunting it’s a nutrition problem in Indonesia, prevalence of stunting in Indonesia is 35.6%. The impact of chronic under nutrition in children can decreased brain development that can result in low intelligence, learning ability in children. The purpose of this study was to identify feeding practices, food consumption and psychosocial stimulation and it’s relation to child development in stunting and normal under five children. Design used in this study was a crosssectional study. Subjects in this study were 92 children under the age of 36-60 months in the village Cibatok Dua. The result showed, there was significant differences in food consumption and language development between stunting and normal under five children (p<0.05). Correlation test result is significant positive relation between food consumption and nutritional status (HAZ), nutritional status (HAZ) and cognitive development, and nutritional status (HAZ) and language development (p<0.05).
Keyword: child development, food consumption, feeding practice, stunting

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.