Judul: Sustainability Study of the Pantura Jawa Urban Area on the  Phenomenon of Land Subsidence
Abstrak: Proses urbanisasi dan perkembangan perekonomian Pulau Jawa sebagian besar di kota Pantai Utara Jawa (Pantura Jawa), akibatnya menimbulkan dampak kerusakan lingkungan samakin parah. Salah satu kerusakan lingkungan di Pantura Jawa adalah rentan terhadap penurunan muka tanah yang diakibatkan oleh aktivitas alam maupun manusia. Penurunan muka tanah terjadi di kota Pantura Jawa dimana penurunan tertinggi terjadi di Jakarta, Pekalongan, dan Semarang. Dampak permasalahan ini adalah retaknya bangunan dan infrastruktur serta perluasan daerah banjir rob, sehingga mengganggu aktivitas masyarakat. Untuk itu sangatlah penting menyelidiki penurunan muka tanah dan faktor penyebabnya, serta menyusun strategi adaptasi dan mitigasi. Tujuan penelitian ini adalah untuk menilai status keberlanjutan wilayah Pantura Jawa terhadap fenomena penurunan muka tanah berdasarkan laju penurunan tanah, identifikasi faktor penyebab, serta penilaian aspek keberlanjutan. Hasil penilaian status keberlanjutan menghasilkan rekomendasi strategi terhadap fenomena penurunan muka tanah. Metode yang digunakan untuk mendapatkan laju penurunan muka tanah adalah Permanent Scatterer Interferometric Synthetic Aperture Radar (PS-InSAR) dengan data radar Sentinel-1A. Metode yang digunakan untuk mengidentifikasi faktor kunci penyebab penurunan muka tanah adalah Matrix of Cross Impact Multiplications Applied to a Classification (MICMAC). Selanjutnya untuk menilai status keberlanjutan menggunakan metode Multiaspect Sustainability Analysis (MSA) dengan inputan data tiga aspek utama pembangunan berkelanjutan yaitu aspek ekologi, ekonomi dan sosial. Untuk rekomendasi strategi keberlanjutan diperoleh dari hasil analisis penilaian aspek keberlanjutan. Hasil penelitian menunjukkan bahwa tiga kota Pantura Jawa masih mengalami penurunan muka tanah dengan nilai rata-rata di Jakarta sebesar -5,64 cm/tahun, Semarang -4,48 cm/tahun, dan Pekalongan -4,26 cm/tahun. Faktor kunci yang menyebabkan penurunan muka tanah adalah ekploitasi air tanah berlebihan dan konsolidasi alami tanah aluvial. Hasil penilaian status keberlanjutan di tiga kota Pantura Jawa, menghasilkan nilai indeks dibawah 50% di masing-masing kota yang termasuk kategori kurang berkelanjutan. Dari penilaian menghasilkan faktor pengungkit yang menjadi dasar rekomendasi strategi di setiap kota. Untuk wilayah Jakarta adalah peningkatan kuantitas dan kualitas ruang terbuka hijau, penataan kawasan peruntukan industri, dan penyediaan air bersih rumah tangga dan industri. Di Pekalongan, strategi penerapan sumur resapan serta penyediaan pasokan air permukaan untuk penduduk, sedangkan di wilayah Semarang adalah rehabilitasi dan konservasi mangrove, pembangunan struktur tanggul dan bendungan, dan pengawasan sumberdaya air tanah. Strategi-strategi tersebut sebagai upaya menyusun langkah adaptasi dan mitigasi yang komprehensif dan efektif., The process of urbanization and economic development on the island of Java is mostly in the cities of the North Coast of Java (Pantura Jawa), resulting in increasingly severe environmental damage. One of the environmental damages in Pantura Jawa is that it is susceptible to land subsidence, which is caused by natural and human activities. Land subsidence occurred in the city of Pantura Jawa, where the highest subsidence occurred in Jakarta, Pekalongan and Semarang. The impact of this problem is the cracking of buildings and infrastructure as well as the expansion of tidal flood areas, thus disrupting community activities. For this reason, it is very important to investigate land subsidence and its causes, as well as develop adaptation and mitigation strategies. The aim of this research is to assess the sustainability status of the Pantura Jawa region regarding the phenomenon of land subsidence based on the rate of subsidence, identification of causal
Keyword: MICMAC, MSA, Pantura Jawa, land subsidence, PS-InSAR, sustainability status

Judul: Perencanaan Lanskap Kawasan Permukiman Berbasis Mitigasi Penurunan Muka Tanah Studi Kasus Kecamatan Cengkareng Jakarta Barat
Abstrak: Kecamatan Cengkareng merupakan salah satu kecamatan yang terdapat di Kota Jakarta Barat yang kerap mengalami fenomena penurunan muka tanah. Penurunan muka tanah (land subsidence) merupakan suatu proses gerakan penurunan muka tanah yang didasarkan atas suatu datum tertentu (kerangka referensi geodesi) dimana terdapat berbagai macam variabel penyebabnya (Marfai 2006). Penurunan muka tanah dapat disebabkan oleh beberapa faktor, yaitu faktor alami, faktor pengambilan air tanah dan faktor massa bangunan. Penurunan muka tanah dapat berdampak, baik secara material maupun non-material. Untuk mengatasi dampak yang akan muncul dibutuhkan perencanaan tata ruang yang dapat meminimalisir dampak yang terjadi akibat penurunan muka tanah pada kawasan Kecamatan Cengkareng. Tujuan penelitian ini adalah menyusun rencana lanskap kawasan permukiman berbasis mitigasi bencana penurunan muka tanah. Penelitian dilakukan dengan pendekatan lanskap dalam proses perencanaan lanskap sebagaimana dikemukakan oleh Fabos (1979) yang terdiri tiga tahap, yaitu: penentuan sumber daya kritis, penentuan sumber daya bahaya dan penentuan kawasan permukiman yang dapat dikembangkan. Penelitian dilakukan untuk mengetahui karakteristik kawasan rawan bencana penurunan muka tanah, keterkaitan antara penurunan muka tanah dan bencana banjir, serta menghasilkan perencanaan tata ruang yang dapat menjadi solusi dari permasalahan penurunan muka tanah. Berdasarkan hasil analisis, rencana lanskap dituangkan kedalam empat zona yakni zona konservasi, zona rawan bencana, zona penyangga dan zona pengembangan. Setiap zona dikembangkan secara fungsional dan terintegrasi dengan kebutuhan ruang, fasilitas serta aktivitas masyarakat Kecamatan Cengkareng., Cengkareng District, located in West Jakarta City, experiences frequent land subsidence phenomenon. Land subsidence is a process of land subsidence movement based on a certain datum (geodesy reference framework) where there are various variables (Marfai 2006). This process is caused by natural, groundwater extraction, and building mass factors. This phenomenon could lead to material and non-material impacts. In order to minimize the negative effects of land subsidence in the area, there is a need for spatial planning. The aim of this research is to develop a landscape plan for residential areas based on land subsidence disaster mitigation. The process involved three stages of landscape planning as proposed by Fabos (1979) determining critical resources, determining hazardous resources and determining residential areas that can be developed. The research was conducted to identify areas prone to land subsidence disasters, the relationship between land subsidence and flood disasters, and to produce spatial planning that can mitigate the problem of land subsidence. Based on the analysis, the landscape plan is divided into four zones: conservation, disaster-prone, buffer and development zones. Each zone is developed with the needs of the community in mind, integrating space, facilities, and activities.
Keyword: Sumber Daya Bahaya, Sumber Daya Kritis, Sumber Daya Pengembangan, Penurunan Muka Tanah, Critical Resources, Development Resources, Hazardous Resources, Land Subsidence

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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