Judul: Penapisan Lektin dari Alga Hijau Asal Pantai Sepanjang, Y ogyakarta dan Binuangeun, Banten
Abstrak: Lectins are carbohydrate binding proteins that are reversible and have ability to agglutinate. Lectins derived from algae have several advantages, such as having low molecular mass, able to bind specific complex oligosaccharides and glycoproteins and they do not require divalent cations for their biological activity. This research aims to screen lectins of green algae from the coastal of Sepanjang, Yogyakarta and Binuangeun, Banten. This research consists of four stages: 1) sampling and identification of green algae; 2) extraction of the algal sample using Tris Buffer Saline (TBS) and Phosphate Buffer Saline (PBS); 3) hemagglutination test of the crude extract using rabbit eritrocytes and group A, B, and O of human eritrocytes, both native and enzyme treated erithrocytes; 4) analysis of total protein content using the BCA protein assay kits. Eleven species of green algae were collected and extracted. Five species of the algae (Cladhopora patentiramea, Ulva fasciata, Halimeda macroalba, Caulerpa racemosa var. macrophysa, dan Halimeda micronesica) showed hemagglutination activity on human erithrocytes. However, hemagglutination test of the other species (Ulva reticulata, Caulerpa sertulaioides, Caulerpa racemosa var. peltata, Chaetopmorha crassa, Enteromorpha intestinalis, dan morfospecies 1.) showed negative result on human erithrocytes. The hemagglutination activity of all the algae was positive on rabbit erithrocytes. Hemagglutination activity of all the algae except Ulva fasciata and Halimeda macroalba on the trypsin treated erithrocytes was lower than the native erithrocytes. A total of 5 species of green algae which showed activity against human erythrocytes, specifically on either O, A, and B blood group. The high total protein content of the algal extract did not positively correlated with the hemagglutination activity.
Keyword: Bogor Agricultural University (IPB), lectins., hemagglutination, green algae, glycoproteins, chlorophyta

Judul: Effect of Algae Extracts on the Growth of Bacillus  subtilis and Pleurotus sp. HS Isolate
Abstrak: Ganggang coklat dan merah banyak dibudidayakan di Indonesia. Ganggang coklat memiliki kandungan senyawa bioaktif dari produk metabolisme sekundernya seperti antimikrob, sedangkan ganggang merah memiliki potensi antioksidan dan penangkal radikal. Penelitian ini bertujuan untuk menguji ekstrak ganggang coklat (Sargassum sp.) dan merah (Gracilaria sp.) sebagai sumber senyawa antimikrob terhadap Bacillus subtilis dan dalam menghambat pertumbuhan koloni Pleurotus sp. isolat HS. Tahapan penelitian ini meliputi persiapan kultur bakteri dan cendawan, persiapan ekstrak ganggang Sargassum sp. dan Gracilaria sp. dengan metode maserasi, uji antimikrob esktrak terhadap B. subtilis dengan metode difusi cakram kertas, dan uji ekstrak terhadap pertumbuhan vegetatif Pleurotus sp. isolat HS pada media Potato Glucose Agar (PGA). Hasil uji antimikrob ekstrak Gracilaria sp. terhadap B. subtilis menghasilkan zona hambat dengan rerata diameter 5.6±1.14 mm dan menghambat pertumbuhan Pleurotus sp. isolat HS koloni sebesar 23%. Adapun ekstrak Sargassum sp. mempunyai aktivitas antimikrob dengan nilai rerata 1.2±1.10 mm terhadap B. subtilis dan menghambat pertumbuhan koloni Pleurotus sp. isolat HS sebesar 16%. Berdasarkan hasil tersebut menunjukkan bahwa ekstrak Gracilaria sp. mampu menghambat pertumbuhan B. subtilis dan Pleurotus sp. isolat HS lebih baik dibandingkan ekstrak Sargassum sp.., Brown and red algae are cultivated in Indonesia. The brown algae contains bioactive compounds from its secondary metabolic products, such as antimicrobials, and red algae has antioxidant with radical scavenging potential. This study aimed to evaluate extracts from brown algae (Sargassum sp.) and red algae (Gracilaria sp.) as sources of antimicrobial compounds against Bacillus subtilis and their inhibitory effect on the growth of Pleurotus sp. isolate HS colony. The stages include preparation of microbes growth media, the algae extracts by macerations, the antimicrobial test of extracts against B. subtilis by paper disc diffusion method, and the test of extracts against vegetative growth of Pleurotus sp. isolate HS on Potato Glucose Agar media. The research result shows that the Gracilaria sp. extract against B. subtilis produced an inhibition zone with an average diameter of 5.6±1.14 mm and inhibited the growth of Pleurotus sp. isolate HS colony by 23%. The extract of Sargassum sp. had antimicrobial activity with a mean value of 1.2±1.10 mm against B. subtilis and inhibited the growth of Pleurotus sp. HS isolate colony by 16%. These results show that the Gracilaria sp. extract can inhibit the growth of B. subtilis and Pleurotus sp. HS isolate better than the Sargassum sp. extract.
Keyword: Antimicrobial, macerations, paper disc, vegetative growth

Judul: Implementasi Space Time Composite (STC) Data Model Menggunakan Model Data Relasional
Abstrak: The data model is a concept that describes the data stored in the database and the relationship between the data to the user in logic. Development of data models is very important in the design of the database as the basis of the stage of database design to be made. This study aims to apply the concept of Space Time Composite (STC) on the Model Data storage vector data types that can store enough information and describes the process of drafting objects and objects spatiotemporal evolution over time. From the results of the test queries on the data model that have been created, queries in a system are acquired that can help display the information preparation of objects from time to time and know the history of the object polygon and arc that has undergone changes in topology.
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