Judul: Konsentrasi CO2 pada Dua Kondisi Kerapatan Tajuk di Taman Kota 1 Bumi Serpong Damai
Abstrak: Permasalahan terkait peningkatan konsentrasi CO₂ sering terjadi di kota-kota besar karena tingginya aktivitas kendaraan bermotor. Keberadan taman kota yang didominasi oleh pepohonan berpotensi menurunkan konsentrasi CO₂ yang diakibatkan oleh aktivitas perkotaan. Penelitian ini bertujuan untuk mengetahui pengaruh perbedaan kondisi kerapatan tajuk, hari, waktu, dan jarak dari pinggir jalan terhadap konsentrasi CO₂. Pengambilan data primer dilakukan dengan tiga kali pengulangan berdasarkan waktu sebagai berikut: 06.00, 13.00, dan 17.00 pada lokasi kerapatan tajuk rendah dan kerapatan tajuk tinggi menggunakan alat Carbon dioxide meter AZ 7725. Kerapatan tajuk rendah memiliki indeks luas daun (LAI) 1,039, sedangkan kerapatan tajuk tinggi memiliki LAI 1,409. Berdasarkan hasil uji T, perbedaan kondisi hari dan waktu memberikan pengaruh signifikan terhadap konsentrasi CO₂, sedangkan perbedaan kerapatan tajuk dan jarak kurang memberikan berpengaruh signifikan terhadap konsentrasi CO₂., Problems related to increasing CO₂ concentrations often occur in big cities because of the high activity of motorized vehicles. The existence of urban parks that are dominated by trees has the potential to reduce CO₂ concentrations caused by urban activities. This study aims to determine the effect of differences in canopy density, day, time, and distance from the roadside on CO₂ concentrations. Primary data collection was carried out with three repetitions based on time as follows: 06.00, 13.00, and 17.00 at locations of low canopy density and high canopy density using a Carbon dioxide meter AZ 7725. Low crown density has a leaf area index (LAI) of 1,039, while the density the high header has an LAI of 1,409. Based on the results of the T test, the difference in day and time conditions had a significant effect on CO₂ concentration, while differences in crown density and distance had less significant effect on CO₂ concentration.
Keyword: canopy density, city park, CO₂ concentration, LAI

Judul: Daya Rosot Karbondioksida Oleh Beberapa Jenis Tanaman Hutan Kota di Kampus IPB Darmaga
Abstrak: The increase of temperature in urban area is indirectly caused by the increase of Carbon Dioxide (CO2) concentration in the atmosphere. The increase is caused by land cover alteration and high amount of human activities which uses fossil fuel. The existence of urban forest is one of the ways to coupe the increase of CO2 concentration in the urban atmosphere. Through photosynthesis process, CO2 gas is absorbed by various plants in urban forest and afterwards being released as Oxygen (O2). To control or reduce the concentration of CO2 effectively, in the establishment of urban forest, selection of plants which have maximum ability in reducing CO2 concentration needs to be done. The objectives of this research are to obtain data CO2 sink ability of 15 urban forest plant species in Bogor Agricultural University and to obtain data about the plant species that has the effective CO2 sink ability among the plants which are measured. The measuring of CO2 sink ability was conducted using carbohydrate method. CO2 mass is obtained by converting carbohydrate mass produced in photosynthesis. Other data taken include leaf area and the number of leaves. The higher the leaf area, the higher CO2 sink ability of a leaf. The higher number leaf of a tree, the higher CO2 sink in a tree. The CO2 sink ability of 15 plant species in cm2 of leaf area are (g CO2/cm2/hour): M. caesia 3,793 x 10-4; D. indica 2,180 x 10-4; B. racemosa 1,600 x 10-4; S. campanulata 1,249 x 10-4; M. champaca 1,176 x 10-4; S. malacense 0,820 x 10-4 ; B. capitella 0,805 x 10-4; C. cauliflora 0,734 x 10-4; V. pubescens 0,669 x 10-4; C. inophyllum 0,629 x 10-4 ; M. ferrea 0,479 x 10-4; A. moluccana 0,357 x 10-4; A. dammara 0,268 x 10-4-; and G. dulcis 0,089 x 10-4. The CO2 sink ability of 15 plant species in urban forest in order from the highest are D. indica, M. caesia, S. campanulata, C. inophyllum, B. racemosa, V. pubescens, M. ferrea, C. cauliflora,B. capitella, G. dulcis, M. champaca, S. malacense, A. moluccana, and A. dammara. Keywords: carbon dioxide sink, urban forest.
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Judul: Influenza Specific Immunoglobulin Y Purification from Egg Yolk with Sodium Chloride Precipitation Method
Abstrak: Imunoglobulin Yolk (IgY) yang terdapat dalam kuning telur telah banyak dimanfaatkan untuk imunoterapi dan imunodiagnostik. Salah satu kendala dalam pemurnian IgY dari kuning telur adalah pemisahan Ig dengan lemak, sehingga teknik pemurnian Ig selalu berkembang. Penelitian ini bertujuan untuk melakukan pemurnian IgY dari kuning telur dengan metode pengendapan menggunakan sodium chloride (NaCl) serta melakukan karakterisasi IgY spesifik influenza. Sodium chloride dipilih karena bahan kimia ini relatif murah, ramah lingkungan, dan tidak merusak imunoaktivitas IgY. Kuning telur yang digunakan dalam penelitian ini berasal dari telur ayam layer yang divaksinasi dengan vaksin influenza Flubio® yang berisi virus Influenza A/H3N2, A/H1N1 dan B/Washington. Pemurnian IgY hanya dilakukan pada kuning telur yang mengandung antibodi spesifik influenza. Deteksi keberadaan antibodi spesifik influenza ditentukan dengan uji agar gel presipitasi (AGP) dan metode enzyme linked immunosorbent assay (ELISA). Konsentrasi IgY hasil pemurnian dihitung dengan spektrofotometer dan karakterisasi protein dilakukan dengan metode Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Konsentrasi protein dari hasil pemurnian IgY memiliki nilai rata – rata 50,403 mg/mL. Hasil SDS-PAGE menunjukkan enam pita protein, yaitu struktur IgY utuh (174,63 kDa), Apoprotein-LDL (129,77 kDa), rantai berat IgY (66,54 kDa), Fab ( 47,65 kDa), rantai ringan IgY (21,85 kDa), dan β-livetin (36,74 kDa). IgY hasil pemurnian masih dapat berikatan dengan antigen influenza berdasarkan uji ELISA. Hasil penelitian ini menunjukkan bahwa pemurnian IgY menggunakan metode pengendapan NaCl dapat memurnikan IgY dengan struktur utuh, meskipun masih ada dua protein tidak spesifik hasil pemurnian. IgY yang dapat berikatan dengan antigen influenza mengindikasikan prospek pemanfaatannya untuk pencegahan influenza dan imunodiagnostik., Yolk immunoglobulin (IgY) contained in egg yolk has been widely used for immunotherapy and immunodiagnostics. One of the obstacles in IgY purification from egg yolk is separating Ig from fat, so that IgY purification techniques are always developing. This study aimed to purify IgY from egg yolk using sodium chloride (NaCl) precipitation method and to characterize influenza-specific IgY. Sodium chloride was chosen because this chemical is relatively unexpensive, environmentally friendly, and does not impair IgY immunoactivity. The egg yolks used in this study were collected from layer eggs vaccinated with the Flubio® influenza vaccine, which contains the Influenza A/H3N2, A/H1N1 and B/Washington viruses. IgY purification was only carried out on egg yolks containing influenza specific antibodies. The presence of influenza-specific antibodies was determined by the agar gel precipitation (AGP) test and the enzyme linked immunosorbent assay (ELISA) method. The purified IgY concentration was determined using spectrophotometer, and protein characterization was performed using the Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) method. Protein concentration from purification has a mean value of 50,403 mg/mL . SDS-PAGE result showed six protein bands, namely intact IgY structure (174,63 kDa), Apoprotein-LDL (129,77 kDa), IgY heavy chain (66,54 kDa), Fab (47,65 kDa), IgY light chain (21,85 kDa), and β-livetin (36,74 kDa). The purified IgY still able to bind with influenza antigen based on the ELISA test. The results of this study indicate that purification of IgY using the NaCl precipitation method can purify IgY with intact structure, although there are still two unspecific proteins in the purification results. The resulting IgY can bind to influenza antigens indicating the
Keyword: Immunoglobulin Y, influenza, egg yolk, purification, sodium chloride