Judul: Analisis Kepuasan dan Loyalitas Konsumen Jigoku Ramen, Bandung, Jawa Barat
Abstrak: This study aims (1) to analyze the characteristics of consumers in Jigoku Ramen, (2) to analyze the buying decision process, (3) to analyze the level of consumer satisfaction, and (4) to analyze the level of consumer loyalty. The sampling method used was convenience sampling. Descriptive analysis, Importance Performance Analysis (IPA), Customer Satisfaction Index (CSI), and loyalty pyramid were employed in this study. There were 100 respondents participated on this research. The major respondents were female customers of 19-24 years old who live in Bandung, mostly students, single marital status, and high school graduates. Their average income per month was IDR500 000–IDR1 500 000, and their average spending in Jigoku Ramen was IDR50 000–IDR100 000. The descriptive analysis described the level of needs, the source of information about the restaurant, the evaluation of alternative options, the consuming process, and after-consuming process. The analysis of Customer Satisfaction Index (CSI) showed that this restaurant was rated by 72.48 percent. It means that the most costumers were satisfied by Jigoku Ramen’s performance. The consumer loyalty was rated by 91 percent, meaning that consumers were mostly loyal buyer.
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Judul: Analisis Tingkat Kepuasan dan Loyalitas Konsumen Restoran Sushi Nest Bogor, Jawa Barat
Abstrak: Restoran Sushi Nest merupakan salah satu restoran yang menawarkan menu masakan Jepang dengan menu utama sushi dengan harga relatif terjangkau oleh kalangan pelajar dan mahasiswa. Tujuan dari penelitian ini adalah untuk menganalisis proses keputusan pembelian konsumen, tingkat kepuasan konsumen, dan loyalitas konsumen yang akan memberikan implikasi terhadap bauran pemasaran dari Restoran Sushi Nest. Metode yang digunakan dalam penelitian ini adalah metode analisis deskriptif, Customer Satisfaction Index (CSI), Importance Performance Analysis (IPA), dan piramida loyalitas. Lokasi Restoran Sushi Nest berada di Jalan Mega Mendung No. 3, Bogor. Karakteristik konsumen dari Restoran Sushi Nest dengan mayoritas berjenis kelamin perempuan yang berasal/domisili di Bogor dengan usia 19-24 tahun dan berprofesi sebagai pelajar/mahasiswa, berpendidikan terakhir SMU, berstatus belum menikah, dan memiliki pendapatan/uang saku per bulan sebesar Rp 1 000 000 sampai Rp 2 000 000. Pada tahap keputusan pembelian (pembelian terencana, sumber yang mempengaruhi adalah teman, hari berkunjung pada hari kerja, dan waktu berkunjung adalah saat malam hari), dan keputusan pasca pembelian (konsumen merasa puas dan hal tersebut mempengaruhi konsumen untuk berkunjung kembali). Nilai dari Costumer Satisfaction Index (CSI) adalah sebesar 72 persen. Konsumen merasa puas terhadap produk restoran namun cenderung tidak loyal terhadap Restoran Sushi Nest.
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Judul: Gene Editing  of Two Cyanide Synthesis-related Genes in Cassava using CRISPR/Cas9
Abstrak: Cassava (Manihot esculenta) contain toxic quantities of cyanogenic glucoside. Reducing the potentially toxic cyanogenic glucoside levels in cassava could be achieved by gene editing through Clustered Regularly Interspaced Short Palindromic Repeats – CRISPR associated protein 9 (CRISPR/Cas9), a high- precision genome editing technique in plants. Two cassava UDP- glucosyltransferase (UGT) paralogs, UGT85K4 and UGT85K5, are the genes catalyzing the synthesis of cyanogenic glucosides in plants. The study aimed to construct the CRISPR/Cas9 recombinant vector carrying single guide RNA (sgRNA) to edit UGT85K4 and UGT85K5 genes, and transform sgRNA CRISPR/Cas9 construct into KU50 cassava calli. Five sgRNAs were designed, considering the on/off target efficiency score and GC content. Those sgRNAs were ligated into pRGEB32 plasmid and introduced to competent Escherichia coli DH5a. The PCR colony and DNA sequencing were performed to verify the sgRNA insert. The amplicons showed the expected band size of recombinant vector (180 bp). Based on sequencing results, only one sgRNA was successfully ligated into the plasmid that will be used to edit the UGT85K5 gene. The sgRNA CRISPR/Cas9 construct in pRGEB32 plasmid was successfully transformed into Agrobacterium tumefaciens and introduced into KU50 cassava calli through co-cultivation. The overgrowth of Agrobacterium occurred after the co-cultivation phase.
Keyword: CRISPR/Cas9, Cyanogenic glucoside, Gene editing, sgRNA