Judul: Analysis of Soil Physical and Chemical Properties and Their Relationship with the Abundance of Soil Mesofauna and Macrofauna in Mount Gede Pangrango National Park
Abstrak: Kelimpahan fauna tanah dipengaruhi oleh berbagai faktor lingkungan meliputi faktor edafis, klimatis, dan keberadaan vegetasi di atasnya. Penelitian ini bertujuan menganalisis kelimpahan fauna tanah pada ketinggian tempat berbeda serta menganalisis pengaruh lingkungan terhadap kelimpahan fauna tanah. Penelitian dilaksanakan pada tiga ketinggian yang berbeda yaitu 1.550 m dpl, 1.650 m dpl, 1.750 m dpl, serta di lahan terbuka (1.200 m dpl). Hasil uji BNT menunjukkan kelimpahan fauna tanah yang berbeda nyata antara ketinggian 1.650 m dpl dengan lahan terbuka, sementara kelimpahan fauna tanah pada ketinggian 1.550 m dpl dan 1.750 m dpl tidak berbeda nyata pada selang kepercayaan 95%. Tutupan vegetasi turut memberikan kontribusi dalam kelimpahan fauna tanah dan dibuktikan dengan lahan terbuka yang memiliki kelimpahan fauna tanah terkecil. Hasil analisis korelasi menunjukkan bahwa intensitas cahaya dan suhu udara berkorelasi negatif, sedangkan kelembaban udara berkorelasi positif dengan kelimpahan fauna tanah. Faktor yang berkorelasi kuat dengan kelimpahan adalah berat basah serasah dan pH tanah., The abundance of soil fauna is influenced by various environmental factors including edaphic factors, climatic factors, and the presence of overlying vegetation. This study aims to analyze the abundance of soil fauna at different altitudes and analyze the influence of the environment on the abundance of soil fauna. The research was conducted at three different altitudes, namely 1.550 m asl, 1.650 m asl, 1.750 m asl, and in open land (1.200 m asl). The LSD test results showed that the abundance of soil fauna was significantly different between the altitude of 1,650 m asl and open land, while the abundance of soil fauna at an altitude of 1,550 m asl and 1,750 m asl was not significantly different at the 95% confidence interval. Vegetation cover also contributes to the abundance of soil fauna as evidenced by open land having the smallest abundance of soil fauna. The results of the correlation analysis show that light intensity and air temperature are negatively correlated, while air humidity is positively correlated with the abundance of soil fauna. Factors that are strongly correlated with abundance are litter wet weight and soil pH.
Keyword: altitude, climatic, diversity, edaphic, vegetation

Judul: Kelimpahan Fauna Tanah dan Hubungannya dengan Karakteristik Tapak pada Vegetasi Submontana di Taman Nasional Gunung Gede Pangrango
Abstrak: Fauna tanah merupakan komponen biotik tanah sebagai bioindikator kesuburan. Perbedaan ketinggian di Taman Nasional Gunung Gede Pangrango diduga mempengaruhi kelimpahan fauna tanah. Penelitian bertujuan mengidentifikasi perbedaan kelimpahan mesofauna dan makrofauna tanah di lokasi penelitian dan menganalisis hubungan faktor klimatis serta edafisnya. Analisis vegetasi dilakukan pada petak contoh 20 m x 20 m yang ditempatkan secara purposive sampling pada ketinggian 1.250, 1.350, 1.450 m dpl dan lahan terbuka (1.200 m dpl). Pengambilan sampel tanah dan pengukuran faktor lingkungan dilakukan pada subpetak contoh berukuran (1 m x 1 m). Hasil uji beda kelimpahan fauna tanah menunjukkan bahwa pada lokasi 1.250 dan 1.450 m dpl tidak berbeda nyata, 1.350 m dpl dan lahan terbuka menunjukkan perbedaan nyata. Kelimpahan fauna tertinggi terdapat pada lokasi 1.350 m dpl. Kelimpahan fauna terendah ditunjukkan pada lahan terbuka. Hubungan kelimpahan fauna tanah dengan faktor klimatis berupa suhu lingkungan dan intensitas cahaya memiliki nilai korelasi negatif, sedangkan kelembaban menunjukkan nilai positif. Faktor edafis seperti pH, Kapasitas Tukar Kation, memiliki nilai korelasi negatif dengan kelimpahan, sedangkan berat serasah, respirasi dan porositas tanah menunjukkan nilai korelasi positif.
Keyword: climatic, edaphic, population, soil fauna, submontane

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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