Judul: Pembuatan dan Penentuan Umur Simpan Minuman Hidrolisat Daging Kambing Kacang (C. aegragus hircus)
Abstrak: Goat is one of farming commodity that has considerable potential and opportunities to be developed in Indonesia. Hydrolysis of protein in goat’s meat will produce meat hydrolyzate with better solubility and digestibility than goat meat. Meat hydrolizate has been used for making beverage. The problem that occurs is the smell of goat meat which less preferred. In this study, beverage products will be made from goat meat hydrolyzate with the addition of orange essens. The most preferred formula is by addition of 0,09% fruit essens. This product was made by two heat treatment, sterilization and pasteurization. This product has 7,1 of pH value, 8,0 brix of total soluble solid, and Yellow Red in colour. Meaty flavor that still appears on this product causes the lacking of acceptance in taste parameters, but overall, this product is acceptable by the panelists (overall score of 5.03 for sterilized product and 5,00 for pasteurized product). The protein digestibility of sterilized product is 10,11%, whereas the pasteurized product has 1,52% protein digestibility. The protein digestibility of this product was count relatively to the digestibility of casein (100%). The self life of sterilized product is 164 days in chiller storage and 43 days in room storage based on total plate count of the product.
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Judul: Karakterisasi Peptida Inhibitor Angiotensin Converting Enzyme (ACE) Hidrolisat Daging Kambing Kacang (Capra aegagrus hircus Linn) dalam Potensinya sebagai Ingredien Pangan Fungsional
Abstrak: The kacang goat (Capra aegagrus hircus) is indigenous to Indonesia and Malaysia, and is common throughout in Southeast Asia. The negative issue facing Indonesian people is that consumption of goat meat can lead to hypertension. Natural angiotensin converting enzyme (ACE) inhibitory peptides from animal protein are considered as an alternative antihypertensive agent. ACE inhibitory peptide could be generated by hydrolysis the protein sources by using the proteolytic enzymes. The purpose of this research was to obtain the water soluble fraction of protein hydrolysates derived from kacang goat in their inhibition of ACE and antihypertensive activities. This study used ten male of kacang goats aged 8-24 months from a local farm in East Java were brought to Bogor by Mitra Tani Farm, West Java Indonesia. The goats divided into two slaughter ages, namely < 1.5 years old and >1.5 years old. Kacang goats are generally slaughtered for consumption at these ages. The body part used for this research was the carcass leg. The contents of protein, fat, moisture, ash, and amino acid in the goat meat were not different on both of slaughter ages. The dominant amino acids found in the goat meat were glutamic acid, aspartic acid, leucyne and lysine. Protein hydrolysate of kacang goat meat was generated by adding endoprotease into homogenate (minced meat to distilled water, 1:3) with several concentrations (0.5%, 1.0%, 1.5% w/w of the total substrate) and hydrolyzed for 60 min. The next hydrolysis was conducted by adding protease complex at several concentrations (0.5%, 1.0%, 1.5% w/w of the total substrate) to the first hydrolysate. Digestion proceeded for 1, 3, and 5 h. The pH of homogenate was kept constant by addition of 6 N NaOH and was monitored every 30 min during hydrolysis. The water soluble fraction (WSF) of hydrolysate was obtained by separating the supernatant and its residue. The crude hydrolysates were charactherized by analysis the ACE inhibitory activity, degree of hydrolysis, soluble protein content, peptide content and yield percentage. The ACE inhibitory activity of crude hydrolysate at 0.5 mg/mL and 1.0 mg/mL ranged between 21.2-45.2% and 48-58%, respectively. The crude hydrolysates had degree of hydrolysis ranged between 10.4-26.3%, soluble protein contents ranged between 534.7 - 579.9 mg/g, peptide contents 400.4-520.8 mg/g and the yield percentage ranged between 49.9-76.0%. The highest ACE inhibitory activity resulted from a hydrolysate digested for 4 h with 5 g kg-1 of both enzymes. The hydrolysate was further fractionated gradually to determine the sequence of ACE inhibitory peptide. Fractionation of protein hydrolysate was started by concentrating the hydrolysate through ultrafiltration system by using centrifugal filter membrane (molecular weight cut-off, MWCO; 3 kDa). Furthermore, an active fraction was separated on a Sephadex G-10 column. The most active fraction was then purified by high performance liquid chromatography- reversed phase (HPLC-RP) gradually with two different types of columns and was obtained an ACE inhibitory peptide with a molecular weight of 478 Da. The ACE inhibitory peptide from most active fraction with IC50 : 12.91 mg/mL (27.0 μM) was identified as tetra-peptide, Phe-Gln-Pro-Ser. Protein hydrolysate and Phe-Gln-Pro-Ser demonstrated the potent antihypertensive activities in spontaneous hypertensive
Keyword: ACE inhibitor, peptida bioactive, protein hydrolysate, meat of kacang goat, antihypertensive

Judul: Teknik penentuan posisi bintang berdasarkan pelat foto overlapping
Abstrak: Bintang adalah salah satu objek langit yang menarik untuk diamati. Untuk itu, pengetahuan tentang posisi bintang sangat diperlukan. Posisi bintang di langit dapat dihitung melalui foto bintang. Fotografi astronomi atau astrometri merupakan ilmu yang mempelajari metode untuk menentukan posisi bendabenda langit seperti bintang berdasarkan pelat foto (Kamp, 1967). Sebuah pelat foto berisi citra bintang-bintang yang sudah diketahui posisinya berdasarkan katalog dan citra bintang-bintang yang belum diketahui posisinya. Posisi bintang yang ada di katalog dinyatakan dengan (a,3). Bintang yang belum diketahui posisinya lebih banyak jumlahnya daripada bintang yang sudah diketahui posisinya. Citra bintang pada pelat foto berupa sebuah titik dalam bentuk tetap. Posisinya pada pelat merupakan hasil pengukuran pada bidang kartesius dan dinyatakan dengan (1;,fl). Hasil pengukuran tersebut kemudian direduksi menjadi koordinat terukur (x,y). Hubungan antara (a,3) dan (x,y) didekati dengan sebuah deret dengan konstanta-konsranta deret merupakan konstanta pelat. Nilai konstanta pelat diperoleh dengan menggabungkan hasil pengukuran posisi bintang pada masing-masing pelat menjadi sebuah persamaan normal (Jefferys, 1963). Hasil akhir dari gugus persamaan normal tersebut dijabarkan dalam bentuk nOlasi matriks partisi dan berupa sebuah sistem persamaan linier Ax = b di mana A adalah matriks jarang, dan x merupakan konstanta pelar yang akan dicari nilainya. Untuk menyelesaikan sistem persamaan linier terse but digunakan metode transformasi given. Konstanta pelat yang sudah diperoleh dengan menyelesaikan sistem persamaan linier di atas, kemudian disubtitusi ke sebuah persamaan untuk memperoleh posisi bintang yang tidak tercantum dalam katalog. .
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