Judul: Perkembangan Praimplantasi Embrio Partenogenetik Mencit (Mus musculus albinus) dengan Perlakuan Trichostatin A dan Sciptaid pada Medium Aktivasi
Abstrak: Partenogenesis merupakan tipe reproduksi aseksual yang biasa terjadi pada hewan tingkat rendah. Secara alami, embrio mamalia diperoleh melalui proses fertilisasi sel telur dan sperma. Embrio partenogenetik mulai digunakan dalam penelitian rekayasa embrio untuk mengatasi permasalahan etika. Ketiadaan materi genetik paternal pada embrio partenogenetik ini menimbulkan permasalahan epigenetika, atau disebut juga genomic imprinting. Oleh karena itu, berbagai cara digunakan untuk mengatasi hal tersebut, salah satunya dengan penggunaan histone deacetylation inhibitor (HDACi). Embrio partenogenetik diaktivasi dengan medium kombinasi strontium (SrCl2) dan cytochalasin B. Pada kontrol positif medium aktivasi tidak ditambah HDACi sedangkan perlakuan pertama ditambah trichostatin A (TSA) dan perlakuan kedua ditambah scriptaid. Hasil penelitian menunjukkan bahwa sebanyak 80,0% embrio membelah menjadi 2 sel pada kontrol, 85,0% pada perlakuan pertama, dan 95,0% pada perlakuan kedua. Meskipun tidak berbeda nyata (P>0.05) penambahan HDACi cenderung meningkatkan aktivasi embrio dibandingkan kontrol dan scriptaid cenderung menghasilkan embrio yang teraktivasi lebih baik dibandingkan TSA. Sebanyak 3,4% embrio mampu membelah hingga mencapai tahap 5 sel pada perlakuan scriptaid dan 1,8% pada perlakuan TSA, sedangkan 12,1% embrio pada kontrol hanya mampu berkembang hingga 4 sel. Embrio partenogenetik yang diaktivasi dengan penambahan scriptaid menunjukkan perkembangan yang cenderung lebih baik dibandingkan dengan TSA dan kontrol meskipun hasil kedua perlakuan dan kontrol tidak berbeda nyata (P>0.05).
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Judul: Suplementasi piruvat dan laktat dalam medium kultur modifikasi m-16 guna meningkatkan perkembangan embrio mencit (Mus musculus albinus) in vitro
Abstrak: Penelitian ini dilaksanakan pada bulan April 1997 hingga bulan Februari 1998 di laboratorium Embriologi Fakultas Kedokteran Hewan Institut Pertanian Bogor. Tujuan penelitian ini adalah untuk mengetahui suplementasi piruvat dan laktat dalam medium kultur modifikasi M-16 guna meningkatkan perkembangan embrio mencit (Mus musculus albinus) in vitro. Manfaat penelitian ini adalah untuk memperoleh medium modifikasi yang ideal dengan konsentrasi yang lebih baik untuk meningkatkan perkembangan embrio mencit in vitro.
Keyword: Mus musculus albinus, Reproduction, Mouse

Judul: Characteristic of Overshooting Top (OT) Based on  Himawari  8  Satellite  as  a  Convective  Cloud  Feature  that  Generates  Extreme Weather in the Java Island Region
Abstrak: Overshooting Top (OT) is a feature at the top of a convective cloud that is associated with extreme weather. Research on OT in tropical Indonesia is still very limited. The existence of geostationary satellites with wide area coverage and fast frequency observations is useful for observing the fast dynamics of the OT life cycle. Therefore, this study intends to analyze the relationship between OT and extreme weather, updraft and thermodynamic conditions of OT when extreme weather occurs, the best objective method for detecting OT in the day time and night time, and the spatiotemporal distribution of OT in the tropical region of Java Island during the 2018-2021 period. Based on visual analysis using visible channel and Himawari 8 sandwich products, 87% of extreme weather events in Java were accompanied by the appearance of OT. It indicates that the presence of the OT pattern is a convection cloud pattern or feature that is closely related to extreme weather events for the Java Island region. The existence of a time lag between the appearance of OT and extreme weather events on Java Island indicates that the OT of convection cloud can be used as a precursor or nowcasting for the appearance of extreme weather. Most of the convective cloud OT features during extreme weather events on Java Island have altitude that were only able to exceed the altitude of the equilibrium level and few were able to exceed the tropopause level of 9 km – 16 km. The difference in atmospheric conditions during extreme weather events on Java Island, which is in the tropics and subtropics, is a strong and unstable convective energy condition characterized by a larger CAPE, as well as unstable KI, LI, SI and TTI values. It is offset by kinematic factors in the form of a weak vertical wind shear (bulk wind shear 0-6 km) and a higher freezing point height. The difference between extreme weather events in the Java Island region and other tropical regions compared to subtropical regions is the strong updraft speed of convection clouds. The IRW Texture COMB algorithm based on the IRW-Texture Gradient modification and the COMB algorithm shows a significant increase in quality for detecting OT and non-OT pixels in Java. Rainy days with rainfall > 50 mm/day are very common in areas with an altitude of > 500 m. On the other hand, the level of convection activity which is indicated by the presence of OT and lightning is significantly reduced in this area. OT of convective clouds appear more frequently during the DJF season and less frequently during the JJA season. OT often appears during the day time and rarely at night time. Most OTs appear in the central part, away from the south and north coasts of Java Island during the day, while OTs that appear near the south and north coasts of Java Island can appear in the morning or afternoon.
Keyword: convective clouds, extreme weather, Overshooting Tops, Himawari 8 Satellite