Judul: Skrining Bioaktivitas dan Penambatan Molekuler Secara In Silico Peptida   Baru dari Protein Ikan Bandeng (Chanos chanos)
Abstrak: In silico approaches integrated with bioinformatics tools have created more efficient and robust methods to overcome current challenges in developing bioactive molecules discovery. Virtually extracted peptides from milkfish (Chanos chanos) are predicted to exhibit antihypertensive and antidiabetic activity from this in silico study. This study aimed to analyze the bioactivity and physiochemical properties of peptides from hydrolyzed protein sequences and to discover the potential angiotensin-converting enzyme (ACE) and Dipeptidyl peptidase-4 (DPPIV) inhibitory of novel peptides from milkfish (Chanos chanos) using machine learning based tools. Nine peptides were predicted to have ACE inhibitor activity and three to be DPPIV inhibitors. The tetra-peptides of ACE-inhibitory peptides (MVWH and PPPS) were predicted to possess a competitive mode of ACE inhibitor by directly binding to the Zn-ion tetra-coordinate. The DPPIV inhibitor peptides are predicted to inhibit the DPPIV but with no known specific mode of inhibition. PPPS peptide is the only peptide in this study that satisfied the drug-likeness analysis with no violations of the Lipinski rule of five.
Keyword: ADME analysis, bioactive peptides, in silico, molecular docking

Judul: Angiotensin I-Converting Enzyme (ACE) Inhibition Mechanism of Novel Peptides from Velvet Bean: An In Silico Study
Abstrak: Velvet bean (Mucuna pruriens) peptides are potential ACE inhibitors. This study aimed to identify peptides from velvet bean protein hydrolysate which contribute significantly to ACE inhibitory activity and predict its inhibition mechanism. LC-MS analysis was conducted to identify peptide sequences. Molecular docking was performed to screen for potential ACE inhibitory peptides. Based on the resulting docking scores, four peptides (P1 - EQPRPYPYPRP, P2 - FCRFGVLKV, P3 - QTDEYGNPVHRS, P4 - QTDEYGNPVHR) were selected. Binding mode analysis showed that ACE inhibition by the novel peptides was a combination of competitive and noncompetitive. P1 and P3 were hypothesized to be competitive inhibitors which were able to coordinate with the enzyme’s cofactor (i.e., Zn2+), whereas P2 and P4 were hypothesized to be noncompetitive inhibitors as they were only able to form hydrophobic networks with the binding channels and were unable to coordinate with Zn2+. Furthermore, molecular dynamics simulations demonstrated slight changes in the conformation of the enzyme as a result of peptide binding, confirming the novel velvet bean-based peptides as ACE inhibitors.
Keyword: ACE inhibitor, bioactive peptides, in silico, molecular docking, velvet bean

Judul: Jagorawi Toll Highway's Traffic Accident (1996-1997
Abstrak: Jalan tol Jagorawi adalah jalan tol yang pertama dibangun di Indonesia, menghubungkan antara Jakarta, Bogor dan Ciawi. Jalan tol telah dirancang sedemikian rupa untuk memberi keamanan bagi pemakai jalan, namun kenyataan yang ada memperlihatkan jumlah kecelakaan lalu lintas yang terjadi cukup besar. Penelitian ini bertujuan untuk melihat gambaran kecelakaan lalu lintas di jalan tol Jagorawi mengenai jalur dan ruas tempat kejadian, hari, waktu, serta cuaca saat kecelakaan terjadi. Hasil perhitungan memperlihatkan tingkat kecelakaan di jalur yang menuju arah Jakarta lebih tinggi daripada arah meninggalkan Jakarta. Pada setiap ruas di setiap jalur, tingkat kecelakaan yang mengakibatkan luka-luka jauh lebih tinggi daripada tingkat kecelakaan tanpa korban; namun belum bisa ditentukan ruas mana yang paling berbahaya karena belum ditemui adanya pola tertentu yang khas dari angka-angka hasil perhitungan tingkat kecelakaan per ruas yang ada. Frekuensi kecelakaan di hari Sabtu dan Minggu lebih tinggi dari hari-hari lain, untuk hari Sabtu frekuensi kecelakaan pada jalur yang meninggalkan Jakarta lebih tinggi sedangkan di hari Minggu lebih tinggi pada jalur menuju arah Jakarta. Kecelakaan lebih banyak terjadi pada siang hari, namun kecelakaan fatal lebih banyak terjadi di malam hari. Sebagian besar kecelakaan di jalan tol Jagorawi terjadi pada saat cuaca cerah, di alinemen jalan yang lurus dengan permukaan jalan yang kering.
Keyword: tingkat kecelakaan, statistika deskriptif, uji khi kuadrat