Judul: Variasi genetik jati pada hutan tanaman di Jawa berdasarkan penanda RAPD (Random Amplified Polymorphic DNA)
Abstrak: Hutan jati merupakan aset yang sangat penting khususnya bagi Perum Perhutani dan umumnya untuk negara Indonesia. Permintaan yang tinggi terhadap produk hasil olahan kayu jati menyebabkan harga kayu jati terus meningkat. Meningkatnya permintaan akan kayu jati tersebut melebihi jumlah yang dapat diproduksi secara lestari dari hutan produksi. Kondisi tersebut akhirnya memperbesar jumlah antara jumlah pasokan dan permintaan. Kesenjangan antara permintaan dan produksi ini dapat dipenuhi dengan peningkatan produktivitas hutan jati melalui peningkatan kualitas dan kuantitas bibit.
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Judul: Variasi Genetika Jati Jawa Berdasarkan Metode Random Amplified Polymorphic DNA (RAPD)
Abstrak: Information on genetic variation of teak in Java at present is still limited. Existing data mostly were based on isozyme analysis, on few localized population, and using leaves as sample materials. In this study usage of RAPD (Random Amplified Polymorphic DNA) as one of DNA markers, and teak wood as material to study teak genetic diversity could enrich the available information. Samples were collected from nine Forest Management Unit (FMU) representing three provinces in Java, i.e. FMU Banten, FMU Ciamis and FMU Indramayu of West Java province, FMU Cepu, FMU Kebonharjo, FMU Kendal and FMU Randublatung of Central Java province, FMU Bojonegoro and FMU Ngawi of East Java province. Leaves DNA were extracted using CTAB method and woods DNA were extracted using CTAB (Cethyl Trimethyl Amonium Bromide) method, while RAPD analysis were done using four selected primers, i.e. OPY2, OPY9, OPO13 and OPY11 . Generated data were then analyzed using POPGENE version 3.2 and NTSYS 2.02. Results showed that teak genetic diversity in Java as measured from heterozygosity level ranged from 0,0614 (FMU Ngawi) to 0,2753 (FMU Indramayu). Dendrogram based on calculated Nei’s genetic distances clustered the populations into two main groups, i.e. West Java province and Central-East Java province. Amplified DNA from wood comprised only of small size fragments (maximum 500bp) as compared to those of leaves (maximum 2500bp). If wood is going to be used as material for fingerprinting traded forest products, a refinement method in DNA analysis should be done
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Judul: Case Study of Gallbladder Mucocele and Treatment Used In Small Animals
Abstrak: Gallbladder mucocele (GM) is defined as distention of the gallbladder caused by an inappropriate accumulation or deposition of high concentrations of bile. The purpose of this case study is to study GM conditions in small animals and to report the clinical sign and characteristics of GM through the sonogram interpretation and blood chemistry test evaluation, and studies related to the therapy given and other treatments recommended. A 14-year-old Toy Poodle dog has been diagnosed with severe GM and cholecystitis. No pain signs were shown during the physical examination as asymptomatic is a common characteristic in the early stage of biliary related diseases. In this study Abdominal ultrasonography interpretation and blood chemistry evaluation was conducted in 2021-2022 and analyzed as a secondary data source. Ultrasonography results showed presence of hyperechoic, immobile deposits in gallbladder accompanied by wall thickening and enlarged in size, followed by a significant increase in the enzyme alkaline phosphatase (ALP). This study has summarized mainly the diagnosis and treatments of GM in small animals and suitable alternatives treatments were recommended to encounter the GM conditions in small animals. The effectiveness of combined therapy shows the positive impact on the patient, the condition of GM has been under control.
Keyword: ultrasound, gallbladder, biliary sludge, abdominal ultrasonography