Judul: Karakterisasi Gen Ser2 pada Ulat Sutera Domestik (Bombyx mori)
Abstrak: Insect of the family Bombycidae is a beneficial insect because it produces cocoon as a source of natural silk fibers. Silk fibers from Bombyx mori is produced in the silk gland at the stage of last instar larvae before the formation of the cocoon. The main components of silk are fibroin and serisin. Ser2 gene dominates process of fiber formation when the caterpillar is on the growth stage. The study of genes characteristic serisin 2 in B. mori is still limited. The objective of this study was to analyze characteristic of Ser2 gene in silkworm B. mori originated from Candiroto. Middle silk gland (MSG) of the silkworm B. mori was extracted for the RNA analysis using the RNeasy Mini Kit (Qiagen). Complementary DNA (cDNA) was synthesized from RNA using Revertaid Transcriptase (Thermo Science Inc). It was then amplified using three pairs of the primers, i.e two pairs of housekeeping genes and one pair of Ser2 genes. The result of Ser2 gene amplification was sequenced to obtain composition of nucleotide bases. Sampels BC8 and BC10 were successfully sequenced and then aligned using Molecular Evolutionary Genetic Analysis software version 4 (MEGA 4.0). To obtain kindship percentage, those sequenced samples were compared with other individuals acquired from gene bank using Basic Local Alignment Search Tool-nucleotide ( BLAST n) program. The results was that B. mori BC8 and BC10 having similarity as high as 100% with Bombyx mori serisin 2 (Ser2) transcript variant 2, mRNA (Genebank: NM001172817.1), Bombyx mori serisin 2 (Ser2) transcript variant 1, mRNA (Genebank: NM001172816.1) dan Bombyx mori serisin 2 precursor (Ser2) gene, complete cds alternatively spliced (Genebank: GQ381286.1) .
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Judul: Characteristics  of  Bombyx  mori  L.  Silkworm Sericin Protein in Different Races and Solvent Concentrations
Abstrak: Protein serisin merupakan produk samping dari industri sutra yang memiliki struktur biokimia dan sifat biofisik yang unik. Penelitian ini bertujuan mengidentifikasi karakteristik protein serisin yang terdapat pada kokon ras ulat sutra Bombyx mori L. dengan konsentrasi pelarut degumming (NaOH) yang berbeda. Penelitian menggunakan rancangan acak kelompok faktorial dengan faktor perbedaan ras ulat sutra dan konsentrasi pelarut degumming (NaOH). Analisis yang dilakukan yaitu analisis rendemen serisin, analisis bobot molekul serisin dan analisis asam amino. Proses ekstraksi serisin menghasilkan rendemen protein serisin sebesar 2,6%±0,5 pada konsentrasi NaOH 0% dan 29,8%±6,57 pada konsentrasi 33,3% NaOH 0,1 N. Hasil penelitian menunjukan bahwa penambahan pelarut NaOH pada proses degumming berpengaruh nyata (P<0,05) pada rendemen serisin yang dihasilkan, sedangkan perbedaan ras tidak berpengaruh nyata pada rendemen serisin (P>0,05). Bobot molekul serisin yang dihasilkan berkisar 15,68- 87,87 kDa. Asam amino protein terlarut serisin dikarakterisasi menggunakan high performance liquid chromatography (HPLC) dan dihasilkan asam amino tertinggi pada glisina (30,87%)., Sericin protein is a by-product of the silk industry which has a unique biochemical structure and biophysical properties silkworm races Bombyx mori L. with different concentrations of degumming solvent (NaOH). The study used a factorial randomized block design with different factors of silkworm race and concentrations of degumming solvent degumming (NaOH). The analyzes carried out were sericin yield analysis, sericin molecular weight analysis and amino acid analysis. The sericin extraction process resulted in sericin protein yield of 2,6%±0,5 at 0% NaOH concentration and 29,8%±6,57 at 33,3% NaOH 0,1 N concentration. The results showed that the addition of NaOH solvent in the degumming had a significant effect (P<0,05) on the yield of sericin produced, while racial differences had no significant effect on the yield of sericin (P>0,05). The molecular weight of sericin produced ranged from 15,68 to 87,87 kDa. The soluble protein amino acid sericin was characterized using high performance liquid chromatography (HPLC) and the highest amino acid yield was glycine (30,87%).
Keyword: Bombyx mori, ras berbeda, serisin

Judul: Perencanan Lanskap Sunda Kalapa, Jakarta Utara Sebagai Kawasan W isata Sejarah
Abstrak: Perkembangan kota Jakarta dari waktu ke waktu sangatlah cepat, sehingga menyebabkan kota-kota tua banyak yang tergusur untuk dijadikan daerah baru yang lebih modern. Apabila hal tersebut tidak ditanggulangi secara dini maka keberadaan Kota Lama Jakarta akan hilang. Hal inilah yang melatar belakang untuk dilakukan tindakan perlindungan dan revitalisasi kawasan bersejarah Sunda Kalapa.
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