Judul: Desain Water Park di Kemang, Kabupaten Bogor
Abstrak: Meningkatnya angka jumlah penduduk di Kemang, Kabupaten Bogor menghadapi dampak yang nyata terhadap pertumbuhan penduduk menyebabkan semakin banyaknya kawasan terbangun yang tidak memiliki ruang terbuka hijau. Hal ini memperburuk kondisi keberadaan ruang terbuka hijau yang semakin hari semakin berkurang persentasenya sehingga penting bagi pemerintah maupun pihak swasta bekerja sama dalam meningkatkan ruang terbuka hijau di Kemang, Kabupaten Bogor. Menghadirkan kawasan wisata baru berupa waterpark menjadi salah satu daya tarik wisata utama di Kemang, Kabupaten Bogor yang dapat menjaga dan mempertahankan keberadaan ruang terbuka hijau. Belakangan ini, trend waterpark global yang mengutamakan nuansa alam menjadi pilihan populer bagi pengunjung yang mencari hiburan dan kesegaran. Pengembangan waterpark di Kemang mendukung pertumbuhan ekonomi dan pariwisata daerah, sejalan dengan misi pembangunan kepariwisataan Bogor. Adapun tujuan penelitian ini yaitu menganalisis kondisi eksisting berdasarkan potensi dan kendala yang terdapat pada tapak dan mendesain waterpark untuk pengembangan wisata baru di Kemang, Kabupaten Bogor. Metode yang digunakan dalam penelitian ini adalah metode deskriptif melalui survei atau pengamatan lapang, studi pustaka, dan wawancara. Pendekatan desain yang digunakan adalah program-based design. Hasil penelitian menghasilkan konsep dasar waterpark berupa “tropical paradise oasis” dengan konsep desain yang mengadaptasi bentuk bambu sebagai elemen utama dan salah satu jenis tanaman tropikal yang digunakan pada waterpark ini. Desain tapak ditujukan untuk menawarkan tempat hiburan dengan berbagai wahana air sekaligus healing, serta menawarkan pengalaman holistik yang mencakup aktivitas leisure (bersantai) dan culinary (kuliner).
Keyword: Landscape design, oasis, recreation, tropical garden, water garden

Judul: Landscape Design Situ Jatijajar as Water Tourism Area of Depok City
Abstrak: Danau adalah salah satu lahan basah dengan sistem airnya yang tergenang. Air dalam danau alami atau buatan dapat digunakan untuk kebutuhan manusia dan berbagai macam keperluan hidup manusia. Salah satunya dapat menjadi tujuan rekreasi, dimana keperluan ini dibutuhkan untuk menyegarkan fisik maupun mental manusia. Terdapat beberapa kota yang memiliki fungsi sebagai kota tangkapan air. Kota Depok sebagai salah satunya difasilitasi oleh banyaknya danau dan badan air yang membuat kota ini cocok untuk menjadi kota tangkapan air. Penelitian ini bertujuan untuk menganalisa dan mengembangkan potensi wisata air sehingga dapat membentuk kawasan wisata air yang mempertahankan kondisi sosial dan ekologi lingkungan. Hasil penelitian ini memberikan rekomendasi kepada pemerintah dan pengelola terkait mengenai perancangan kawasan tersebut sebagai wisata air yang sesuai dengan kondisi sosial dan lingkungan. Konsep dasar penelitian adalah sustainable tourism yaitu konsep pengembangan kawasan dengan memperhatikan kondisi lingkungan dan menunjang potensi aktivitas pariwisata. Pemilihan material dan desain fasilitas berbasis pada gambaran pohon jati secara natural dan organik. Hasil penelitian berupa rancangan kawasan yang terbagi menjadi area budidaya, area konservasi, area pelayanan, area penerimaan, area rekreasi serta area wisata dan edukasi yang menunjang berbagai aktivitas dan kebutuhan pengunjung tetapi tetap menjamin batasan konservasi kawasan. Area wisata dan edukasi sebagai area utama kawasan menunjang aktivitas mengelilingi situ menaiki sepeda air atau perahu naga dan menjelajah hutan jati atau melihat pemandangan di menara observasi, Lake is one of the wetlands with its stagnant water system. Water in either natural or artificial lakes can be used for human needs and various purposes of human life. One of them can be a recreational purpose, where this necessity is needed humans to refresh physically and mentally. Several activities have the function of Water Catchment City. Depok City is one of them facilitated by the existence of many lakes and water body that makes suitable as the Water Catchment City. This study intended to analyze and develop the area-based potential of water tourism so that formed a water tourism area that considers the social and ecological condition of the environment. The result of this study provides recommendations for the government and related managers on the design of the area as suitable for water tourism and by social and environmental conditions. The basic concept of the study is Sustainable Tourism, the concept of regional development by taking account of environmental conditions and supporting potential tourism activities. Material selection and facility design are based on the natural and organic image of the teak tree. The result of the study is design which is divided into cultivation area, conservation area, service area, reception area, recreation area as well as tourist and educational area that support various activities and visitor needs but still guarantee the boundaries of area conservation. The tourist and educational area as the main supports activities such as around the lake with water bike or dragon boat and exploring the teak forest and seeing the scenery at the observation tower.
Keyword: lake, water catchment, water tourism

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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