Judul: Kontribusi makanan jajanan, tingkat kecukupan energi dan zat gizi serta morbiditas anak usia sekolah dasar di kota Bogor
Abstrak: Tujuan umum dari penelitian ini adalah untuk mengetahui kontribusi makanan jajanan terhadap tingkat kecukupan energi dan zat gizi serta hubungannya dengan morbiditas pada anak usia sekolah dasar di Kota Bogor. Tujuan khusus penelitian ini antara lain: (1) Mengetahui karakteristik siswa dan orang tua siswa SD Swasta dan SD Negeri, (2) Mengetahui jenis dan frekuensi makanan jajanan yang dikonsumsi siswa, (3) Membandingkan kontribusi makanan jajanan terhadap total konsumsi sehari antara siswa yang bersekolah di SD Swasta dan SD Negeri di Kota Bogor, (4) Menilai dan membandingkan tingkat kecukupan energi dan zat gizi antara siswa yang bersekolah di SD Swasta dan SD Negeri di Kota Bogor, (5) Membandingkan morbiditas antara siswa yang bersekolah di SD Swasta dan SD Negeri di Kota Bogor, dan (6) Menganalisis hubungan konsumsi makanan jajanan dan tingkat kecukupan energi dan zat gizi dengan morbiditas anak usia sekolah. Pemilihan lokasi penelitian dilakukan dengan cara acak atau simple random sampling dengan kriteria berada di wilayah Kota Bogor serta bersedia menjadi tempat penelitian. Kerangka sampling diperoleh dari data Dinas Pendidikan Kota Bogor (tahun ajaran 2008/2009), yaitu 289 Sekolah Dasar (SD). Selanjutnya dipilih 2 SD Swasta dan 2 SD Negeri untuk dijadikan tempat penelitian. SD Swasta yang terpilih adalah Sekolah Dasar Islam Terpadu (SDIT) Aliya dan Sekolah Dasar Swasta (SDS) Pertiwi, sedangkan SD Negeri yang terpilih adalah SDN Baranangsiang dan SDN Kedung Badak I. Penelitian ini dilaksanakan dari bulan April sampai bulan Juni 2009. Contoh dalam penelitian ini adalah siswa kelas 5 SD yang berusia 10-13 tahun. Seluruh siswa tersebut dijadikan sebagai contoh penelitian setelah dikurangi siswa yang drop out karena tidak masuk saat penelitian berlangsung dan data dari siswa yang tidak lengkap. Jumlah contoh dari SD Swasta dan SD Negeri setelah dikurangi siswa yang drop out adalah masing-masing sebesar 120 dan 90 orang.
Keyword: Elementary school children, Street food contribution, Nutrient adequacy level and morbidity

Judul: Kontribusi Makanan Di Sekolah Dan Tingkat Kecukupan Energi Dan Zat Gizi Pada Anak Usia Sekolah Dasar Di Kota Bogor
Abstrak: The objective of this research was to know the contribution of school feeding and home feeding; and to know nutrient adequacy level elementary school children. cross sectional study design was used is in the research which was conducted by using from April to June 2009 in Aliya, Pertiwi, Baranangsiang and Kedung Badak 1 Elementary School Bogor. The samples of this research were 5 grade of elementary school students with and without school lunch program. The contribution of school feeding of samples with school lunch program were higher than samples without school lunch (p<0,05). The contribution of home feeding of samples with school lunch program were lower than samples without school lunch service (p<0,05). Adequacy level of energy, vitamin A and C of school with lunch services (p<0,05). The average level of protein, calcium, phosphor, and iron of samples with and without school lunch program were not different. These result elaborate that the lower contribution of feeding school of student without school lunch program was compensated by home feeding.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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