Judul: Analisis Jumlah Kromosom Biji Ungu Hasil Persilangan Jagung Varietas Pulut dengan BC2 Putatif Penginduksi Haploid dan Hasil Perlakuan Kolkisin
Abstrak: Bulbosum method is very potential to produce haploid plant of maize. The development of Bulbosum method in Indonesia was done until initial step, that is cross between local variety with haploid inducer line. This research was conducted to analyze the chromosome number of purple seed from a cross between Pulut and BC2 putative haploid inducer and from in vitro colchicine treatment. The chromosome number of these purple seed were found not haploid (x=10) but diploid (2n=2x=20). The effect of colchicine with different concentrations and durations of treatment to ploidy level was not be able to be observed, because death happened at almost all of the seeds and seedlings treated. Chromosome doubling was found at treatment of seedling root by colchicine concentration of 0.06% for 6 hours, that is became tetraploid (2n=4x=40), but the plant could not survive until planting in greenhouse. The only plant survived after colchicine treatment until planting in greenhouse was still diploid with morphological and anatomical characters which were not different from control plants.
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Judul: Induksi Mutasi Melalui Penggandaan Kromosom Nilam (Pogostemon cablin Benth.) Varietas Sidikalang dengan Kolkisin secara In Vitro
Abstrak: The research aimed to study the in vitro genetic mutation induction through chromosome doubling of patchouli (Pogostemon cablin Benth) using colchicine. This research was conducted from February 2011 toDecember 2011 at Biotechnology and Micro Technique Laboratory, Department of Agronomy and Horticulture, IPB, Bogor. The research was used factorial design which arranged with Completely Randomized Design. The research was consist of 2 factors, concentrations of colchicine (0 %, 0,02 %, 0.04 % and 0.06 %) and the long immersion with colchicine (24 hours, 48 hours, and 72 hours). The experiment showed that consentrations of colchicine (0.02%, 0.04%, and 0.06%) were significantly affected to increase number of shoots, leaves, chloroplast, stomatas and size of stomata.Concentration of 0.04 % colchicine with 24 immer was produced the highest number of shoots and leave. Concentration of 0.02 % colchicine was produced the highest number of chloroplasts per cell and the lowest density of stomata Concentration of 0.06 % of colchicine and 48 hours immersion was produced the biggest size of stomata. Concentration 0.06 % colchicine with 24 hours immersion and concentration 0.04% colchicine with 72 hour immersion gained chimera. Few shoots had different number of leaves per bud than control. This experiment also can increased phenotypic variance of number shoots and leaves. Concentration of 0.02 % colchicine with 72 hours immersion has the highest coefficent of phenotypic variance and concentration of 0 % colchicine with 24 hours immersion has the lowest coefficient of phenotypic variance. Potential mutant plants which are produced 1189 plants.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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