Judul: Analisis Perdagangan Sumber Daya Mineral Intra-ASEAN dengan Pendekatan Panel Spasial.
Abstrak: Perkembangan zaman pada era globalisasi membuat negara di dunia saling terhubung satu sama lain, salah satu sarana interaksi antar negara tersebut adalah dengan adanya perdagangan internasional. Dalam perdagangan di kawasan ASEAN, sektor sumber daya mineral adalah sektor yang paling mendominasi pasar. Tingginya kontribusi sektor ini tentu dipengaruhi oleh berbagai faktor. Selain itu, dengan kondisi negara ASEAN yang berdekatan maka aspek spasial diduga memiliki pengaruh terhadap perdagangan sumber daya mineralnya. Tujuan dari penelitian ini adalah untuk menganalisis keterkaitan spasial serta faktor-faktor yang memengaruhi perdagangan sumber daya mineral intra-ASEAN. Dalam penelitian ini metode yang digunakan adalah regresi data panel spasial, periode penelitian yang digunakan adalah tahun 2012 sampai 2016 dengan data 10 negara anggota ASEAN. Hasil dari penelitian ini menunjukkan bahwa populasi secara signifikan berpengaruh negatif sedangkan PDB perkapita dan foreign direct investment secara signifikan berpengaruh positif terhadap perdagangan sumber daya mineral intra-ASEAN. Selain itu penelitian ini menyimpulkan bahwa terdapat efek spasial pada perdagangan sumber daya mineral intra-ASEAN.
Keyword: Panel spasial, Perdagangan internasional, Sumberdaya mineral

Judul: Analisis Pengaruh Masyarakat Ekonomi ASEAN terhadap Perdagangan Sumber Daya Mineral Intra ASEAN 7 dengan Pendekatan Panel Spasial
Abstrak: Sektor mineral menjadi salah satu kontributor utama dalam nilai perdagangan intra ASEAN. Tujuan dari penelitian ini adalah untuk menganalisis pengaruh MEA serta faktor lainnya seperti kualitas infrastruktur, stabilitas politik, PDB Perkapita, trade openness, dan nilai tukar nominal pada periode t-1 terhadap nilai perdagangan sumber daya mineral intra ASEAN 7 yaitu Kamboja, Malaysia, Singapura, Thailand, Indonesia, Filipina, dan Vietnam dengan periode analisis dari tahun 2007 sampai 2017. Data dalam penelitian bersumber dari World Bank, ASEAN Statistic Database, dan Worldwide Government Indicator. Metode yang digunakan adalah panel spasial dengan pertimbangan adanya ketergantungan spasial dalam model berdasarkan hasil uji Moran I. General Spatial Model dipilih sebagai model terbaik. Berdasarkan hasil estimasi GSM, diperoleh kesimpulan bahwa MEA dan nilai tukar nominal pada periode t-1 berpengaruh negatif dan signifikan terhadap nilai perdagangan mineral intra ASEAN 7. Kualitas infrastruktur, PDB Perkapita, dan trade openness berpengaruh positif dan signifikan. Sedangkan stabilitas politik tidak berpengaruh signifikan.
Keyword: Masyarakat Ekonomi ASEAN, Panel spasial, Perdagangan internasional, Sumber daya Mineral

Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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