Judul: Penghambatan Autooksidasi Lipid oleh Ekstrak Maggot Black Soldier Fly (Hermetia illucens)
Abstrak: Stres oksidatif merupakan kondisi ketidakseimbangan antara produksi dan degradasi senyawa radikal bebas di dalam tubuh. Jumlah radikal bebas berlebih dapat diatasi dengan adanya antioksidan. Maggot Black Soldier Fly diketahui dapat mengaktifkan persinyalan antioksidan tubuh sehingga berpotensi dijadikan sumber antioksidan alami. Penelitian ini bertujuan mengidentifikasi metabolit dan menganalisis penghambatan proses autooksidasi lipid oleh ekstrak kasar maggot Black Soldier Fly asal Desa Sindangjaya, Cianjur. Sebanyak total 83 senyawa diidentifikasi pada ekstrak kasar maggot berdasarkan analisis menggunakan Liquid Chromatography Tandem-Mass Spectrometry. Aktivitas antioksidan tertinggi berdasarkan metode thiobarbituric acid dihasilkan oleh ekstrak metanol maggot yang diberi pakan limbah dapur, dengan nilai inhibisi sebesar 94,71% pada konsentrasi 5 μg/mL. Nilai ini jauh lebih tinggi dibandingkan dengan α-tokoferol yang hanya menghasilkan nilai inhibisi sebesar 74,85% pada konsentrasi yang sama. Ekstrak maggot yang diberi pakan limbah dapur memiliki aktivitas antioksidan yang lebih kuat dibandingkan dengan yang diberi pakan limbah pertanian., Oxidative stress is a condition of imbalance between the production and degradation of free radicals in the body. Excessive amounts of free radicals can be counteracted by the presence of antioxidants. The Black Soldier Fly maggot is known to activate antioxidant signaling, making it a potential source of natural antioxidants. This research aimed to identify metabolites and analyze the inhibition of lipid autooxidation process by crude extract of Black Soldier Fly maggot from Sindangjaya Village, Cianjur. A total of 83 compounds were identified in maggot crude extract based on Liquid Chromatography Tandem-Mass Spectrometry analysis. The highest antioxidant activity based on the thiobarbituric acid method was produced by the methanol extract of maggot fed with kitchen waste, with an inhibition value of 94.71% at a concentration of 5 μg/mL. This value was much higher than α-tocopherol which only produced an inhibition value of 74.85% at the same concentration. Maggot extracts fed with kitchen waste had stronger antioxidant activity than those fed with agricultural waste.
Keyword: Antioksidan, Autooksidasi Lipid, Maggot Black Soldier Fly, Metabolit, Metode Thiobarbituric Acid

Judul: Secondary Metabolite Profile and Antioxidant Activity of Black Soldier Fly (Hermetia Illucens) Pupae Extract
Abstrak: Black Soldier Fly (BSF) atau Hermetia illucens adalah salah satu serangga dekomposer alami. BSF mampu mengubah limbah organik menjadi metabolit primer dengan gizi tinggi. BSF memiliki potensi sebagai antioksidan karena kandungan metabolit sekundernya. Senyawa antioksidan pada pupa BSF akan diidentifikasi untuk mengetahui kandungan senyawanya. Penelitian ini bertujuan menentukan pelarut yang tepat untuk mendapatkan senyawa aktif pada pupa BSF. Sampel diekstraksi menggunakan tiga pelarut berbeda. Ekstrak kemudian diuji kemampuan antioksidannya dengan metode DPPH dan diukur IC50. Ekstrak dengan aktivitas antioksidan terbaik akan diuji senyawa penyusunnya menggunakan LC-MS. Hasil penelitian menunjukkan pelarut aseton menghasilkan rendemen tertinggi yaitu 10.73%. Aktivitas antioksidan yang dibuktikan dengan IC50 memberikan hasil terbaik pada ekstrak metanol, dengan rata-rata konsentrasi IC50 sebesar 22 ppb. Berdasarkan hasil LC-MS, ekstrak metanol mengandung senyawa golongan indol dan terpenoid. Senyawa-senyawa ini memberikan aktivitas antioksidan yang tinggi pada semua ekstrak pupa BSF., Black Soldier Fly (BSF) or Hermetia illucens is one of the natural decomposer insects. BSF is able to convert organic waste into primary metabolites with high nutrition. BSF has the potential as an antioxidant because of its secondary metabolite content. Antioxidant compounds in BSF Pupa will be identified to determine the compound content. This study aimed to determine the suitable solvent for obtaining active BSF pupae compounds. Samples were extracted using three different solvents. The extract was then tested for its antioxidant ability using the DPPH method, and the IC50 was measured. The extract with the best antioxidant activity will be tested for its constituent compounds using LC-MS. The results showed that acetone solvent produced the highest yield, 10.73%. Antioxidant activity, as proven by IC50, gave the best results in methanol extract, with an average IC50 concentration of 22 ppb. Based on the LC-MS results, the methanol extract contained indole and terpenoid group compounds. These compounds provide high antioxidant activity in all BSF pupa extracts.
Keyword: antioxidants, BSF, IC50, LC-MS

Judul: Implementation of Spatio-Temporal Change Query in Relational Database.
Abstrak: Geographic object changes in various views. Object changes are recorded using a model of static storage. No historical connection between old version data and new version data has been built. For that, a storage model of spatio-temporal queries that can analyze spatio-temporal changes has been developed. The object changes the geometry are grouped according to the taxonomy of Spatio-Temporal Pattern Change. This research has been able to apply the query patterns change including born, expand, merge, contract, split, normal and die. This query based on geometric (area) changes. The implementation of spatio-temporal change queries represents an object changes continuously with easy, change detection is done by comparing two versions of data. Keyword: spatio-temporal change query, spatio-temporal change pattern.
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