Judul: Pengaruh Literasi Keuangan dan Sosialisasi Orang Tua Terhadap Perencanaan Keuangan Hari Tua pada Generasi Milenial
Abstrak: Generasi milenial di Indonesia pada tahun 2022 merupakan individu dengan usia produktif dan aktif bekerja. Banyak hal yang perlu disiapkan oleh para pekerja termasuk untuk kesejahteraan hari tua. Tujuan penelitian ini adalah menganalisis pengaruh literasi keuangan dan sosialisasi orang tua terhadap perencanaan keuangan hari tua pada generasi milenial. Penelitian ini menggunakan desain cross sectional study dan teknik penarikan contoh secara purposive sampling dengan total 206 responden yang merupakan generasi milenial Indonesia. Hasil penelitian menunjukkan bahwa sebagian besar responden memiliki sikap keuangan pada kategori tinggi, sedangkan pengetahuan dan perilaku keuangan serta perencanaan keuangan hari tua berada pada kategori moderat. Studi ini juga menemukan bahwa sosialisasi finansial dari orang tua masih rendah. Uji regresi menunjukkan bahwa jenis kelamin, pekerjaan, literasi keuangan, serta sosialisasi finansial dari orang tua berpengaruh secara signifikan terhadap perencanaan keuangan hari tua.
Keyword: Generasi milenial, literasi keuangan, pensiun, perencanaan hari tua, sosialisasi orang tua

Judul: Literasi Keuangan dan Pengaruhnya terhadap Pengelolaan Keuangan pada Generasi Millenial
Abstrak: Otoritas Jasa Keuangan (OJK) melalui Survei Nasional Literasi dan Inklusi Keuangan menunjukkan bahwa indeks literasi keuangan masyarakat hanya mencapai 29.66% yang tergolong kedalam kelompok well literate. Jawa Barat sebagai provinsi dengan jumlah penduduk Indonesia terbanyak menjadikan target potensial bagi kegiatan edukasi keuangan OJK. Terlebih provinsi Jawa Barat memiliki total 47% generasi millenial yang mendominasi tenaga kerja. Sebagai generasi yang mendominasi tenaga kerja, generasi millenial dituntut untuk dapat mengelola keuangannya dengan baik. Penelitian ini bertujuan untuk menganalisis tingkat literasi keuangan dan pengelolaan keuangan pada generasi millenial di Jawa Barat serta menganalisis pengaruh literasi keuangan terhadap pengelolaan keuangan generasi millenial. Analisis data menggunakan analisis statistika deskriptif dan analisis Structural Equation Modeling (SEM) LISREL. Hasil penelitian menujukkan nilai literasi keuangan pada generasi millenial sebesar 74.09% yang termasuk kedalam kategori sufficient literate. Nilai pengelolaan keuangan sebesar 3.47 yang termasuk kedalam kategori baik serta terdapatnya pengaruh positif literasi keuangan terhadap pengelolaan keuangan pada generasi millenial.
Keyword: literasi keuangan, millenial, pengelolaan keuangan

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable