Judul: Transformasi Genetik Tanaman Tembakau oleh Gen LFY Kakao (TcLFY) Menggunakan Agrobacterium tumefaciens
Abstrak: LFY is a protein that have important role in flowering. TcLFY gene have been isolated from cacao (Theobroma cacao L.). To examine its role, this gene that is under control of p35S CaMV promoter was introduced in the tobacco plant using Agrobacterium tumefaciens. Transformation efficiency of transgenic plants was 16 % with regeneration efficiency was 27.38%, and produce 4 buds each explant. PCR analyses toward 5 putative transgenic tobacco plants that were taken randomly showed that all of them were transgenic. They also flowered early. Expression analyse of TcLFY gene using PCR showed that transgenic plant expressed the TcLFY gene in the all of its parts. This result showed that transgenic tobacco plant contained TcLFY gene under control of p35S CaMV
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Judul: Uji kinerja promoter gen LEAFY dan AGAMOUS tanaman kakao (Theobroma cacao L.) pada tanaman tembakau (Nicotiana tabacum L.)
Abstrak: Performance of cocoa’s (Theobroma cacao L.) gene promoters, TcLFY and TcAG, in a tobacco plant (Nicotiana tabacum L.) used as a plant model has been tested. The two promoters were introduced into the tobacco plant cells using a modified leaf discs method employing Agrobacterium tumifaciens AGL0. The transformed tobacco plant cells were then regenerated and their morphology were observed periodically. The tobacco plants harbouring the gene promoter TcLFY, during regeneration, showed a leaf morphology similar to that of the positive control. However, the growth rate of tobacco plants inserted with promoter TcLFY was faster compared to that of the positive control. Tobacco plants harbouring gene promoter TcAG, during regeneration, showed imperfect leaf morphology. The growth rate of these tobacco plants was slower than that of the positive control and of the tobacco plants inserted harbouring gene promoter TcLFY. These results indicated that the introduced promoters affect the morphology of the tobacco plants. In order to further confirm the presence of the GFP gene and activation of TcLFY: GFP and TcAG: GFP, a molecular verification test was carried out. Variation of sucrose concentration in the media was made to examine the effect of sucrose on the activity promoters TcAG and TcLFY in tobacco plants. The sucrose concentration tested were 0%, 0,5% and 4% followed by observation on the 3rd and 7th day of cultivation. At 0% sucrose concentration the tobacco plants harbouring promoter TcLFY showed yellowish leaf, while those cultivated in the media with sucrose concentration of 0.5% and 4% grew well and normal. This indicated that sucrose affects the activity of the promoter TcLFY in tobacco plants. Tobacco plants inserted with promoter TcAG did not show significant morphological changes when grown in a medium of 0%, 0,5% or 4% sucrose concentration. This indicated that addition of sucrose does not significantly affect the induction of expression of the AG gene and the activation of the promoter TcAG.
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Judul: Prediksi Kurs Rupiah Terhadap Dollar Amerika Menggunakan Persamaan Schrodinger Nonlinear Diselesaikan dengan Metode Runge Kutta Orde Empat.
Abstrak: Kurs adalah pertukaran antara dua mata uang yang berbeda, yaitu perbandingan nilai atau harga antara kedua mata uang tersebut. Prediksi kurs rupiah dapat dimodelkan dengan persamaan Schrodinger nonlinear yang dihitung dengan metode Runge Kutta orde empat. Parameter yang terdapat dalam persamaan Schrodinger nonlinear dianalogikan ke dalam parameter ekonomi yang diasumsikan sebagai faktor-faktor yang mempengaruhi kurs rupiah. Faktor-faktor tersebut antara lain pergerakan atau pertumbuhan rata-rata kurs rupiah, tingkat inflasi, tingkat suku bunga, kecepatan rata-rata perubahan kurs rupiah, dan Produk Domestik Bruto (PDB). Penelitian ini bertujuan untuk mengetahui nilai prediksi kurs rupiah dari perubahan pergerakan kurs rupiah, sehingga pergerakan kurs rupiah yang fluktuatif akan mempengaruhi nilai prediksinya. Data hasil prediksi nilai kurs rupiah terhadap dolar Amerika dibandingkan dengan data kurs aktual. Hasil prediksi kurs rupiah dari persamaan Schrodinger nonlinear menggunakan metode Runge Kutta orde empat menghasilkan persentase error tertinggi dibawah 5% disetiap bulannya dan menghasilkan nilai Mean Absolute Percentage Error (MAPE) sebesar 0.4786%.
Keyword: kurs rupiah, parameter Schrodinger, Runge Kutta orde empat, Schrodinger nonlinear