Judul: Pemurnian dan Karakterisasi Enzim Glukosa Oksidase dari Isolat Lokal Aspergillus niger (IPBCC.08.610)
Abstrak: Production of various enzymes using microorganisms is one way of developing microorganism biodiversity in Indonesia. For this study, glucose oxidase (GOD) which is widely available for use and purchase in the commercial market was used. This research intended to determine the optimum incubation time for the production of GOD from Aspergillus niger local isolate (IPBCC.08.610) and determine the specific activity of the crude extract and purified enzymes fraction. In addition, it sought to determine the optimum temperature and pH level in GOD reaction and establish the values of Km and vmax. Optimum incubation time for GOD production of A. niger (IPBCC.08.610) was 48 hours with initial velocity of the reaction and the specific activity at 0.80/minute and 3.21 U/mg. Ammonium sulfate with 80% saturation was optimum to precipitate the GOD protein with specific activity of the fraction at 22.18 U/mg and purity levels at 6.92 times. Specific activity of the fraction of dialysis was 21.25 U/mg and the level of purity was 6.63 times. Km and vmax values were 46 mM and 11 U/mg with optimal temperature and pH level for GOD reaction between 37 - 45°C and pH 5.5.
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Judul: Pemurnian dan Karakterisasi Glukosa Oksidase dari Isolat Aspergillus niger (IPBCC 08.610)
Abstrak: Glukosa oksidase (GOD) sedang banyak dikembangkan dalam berbagai aplikasi seperti pada industri makanan, obat-obatan, dan elektronik. Sumber GOD terbaik berasal dari Aspergillus niger karena diketahui memiliki aktivitas spesifik yang tinggi. Penelitian ini bertujuan untuk memurnikan glukosa oksidase dari A.niger (IPBCC 08.610) dengan menggunakan teknik kromatografi kolom gel filtrasi dan karakterisasinya. Pemurnian menggunakan kromatografi filtrasi gel menghasilkan satu fraksi enzim yang memiliki kadar protein lebih tinggi (Fraksi 4) dibandingkan fraksi lainnya. Aktivitas spesifik fraksi hasil kromatografi adalah 31.92 U/mg dengan tingkat kemurnian 8.86 kali dibandingkan dengan ekstrak enzim kasar yaitu 3.61 U/mg. Estimasi bobot molekul hasil analisis metode SDS-PAGE menunjukkan 2 pita protein yang berukuran 161 kDa dan 84 kDa. Adapun nilai Km dan Vmaks glukosa oksidase dengan substrat glukosa adalah 39 mM dan 3.7 x 10-3 mmol/det.
Keyword: Aspergillus niger, glukosa oksidase, Km, Vmaks, kromatografi kolom filtrasi gel, pemurnian, SDS-PAGE

Judul: Implementasi Space Time Composite (STC) Data Model Menggunakan Model Data Relasional
Abstrak: The data model is a concept that describes the data stored in the database and the relationship between the data to the user in logic. Development of data models is very important in the design of the database as the basis of the stage of database design to be made. This study aims to apply the concept of Space Time Composite (STC) on the Model Data storage vector data types that can store enough information and describes the process of drafting objects and objects spatiotemporal evolution over time. From the results of the test queries on the data model that have been created, queries in a system are acquired that can help display the information preparation of objects from time to time and know the history of the object polygon and arc that has undergone changes in topology.
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