Judul: Identifikasi dan Karakterisasi Fragmen DNA Genomik Kakao (Theobroma cacao L.) Hasil PCR  Menggunakan Primer Heterologous Leafy
Abstrak: Cacao, in Indonesia from the social and economically point of view, and important commoditie, through the cacao productivity is still low. The unpreddictable blooming seasons bring about the low productivity of cacao plantations.
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Judul: Isolasi dan Karakterisasi Fragmen Gen LEAFY dari Bantalan Bunga Kakao
Abstrak: Cocoa plant productivity in Indonesia is still low. Information and knowledge at the molecular level about the process of development of regenerative organs of cocoa can be an effective solution to problems associated with the regenerative low productivity of cocoa plants.LEAFY gen is key gene in the shoot meristem transition to flower one. The purposes of the research were to isolate LEAFY gene from cacao’s flower cushion and analyse its expresion in several cacao’s tissues. Isolation of LEAFY gene using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) technique showed that three spesific primer pairs designed based conserved area can amplify the target gene. Futhermore this fragment were inserted into pGEM-T Easy vector then transformed into competent cell E. Coli XL-1 Blue. The result of bioinformatic analysis showed that the fragment of target gene has a high homology to LEAFY gene of other plants in gene bank’s database. Characterization of target gene was initiated with RNA isolation phase from several cacao’s tissues then those were amplified with RT-PCR technique. Amplification at 62 ºC of annealing temperature for 33 cycles was successful to show the existence of LEAFY gene in several cacao’s tissues isolated.
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Judul: Kajian Dimensi Bangunan Spillway
Abstrak: Bangunan spillway merupakan bangunan hidrolika dari suatu bendungan yang berfungsi pada saat keadaan darurat (emergency), yaitu keadaan muka air pada saat volume tampungan banjir maksimum. Penelitian ini bertujuan untuk melakukan kaji ulang bangunan spillway pada Bendungan Randugunting berdasarkan kajian terhadap desain existing bangunan spillway, debit rencana bangunan spillway, kedalaman aliran kritis, dan profil muka air bangunan spillway. Tipe bangunan spillway yang direncanakan adalah tipe side spillway dengan tipe mercu Ogee. Hasil analisis luas catchment area Sungai Banyuasin adalah 17.39 km2 dengan panjang sungai 8.52 km. Nilai curah hujan rencana dengan periode ulang 1000 tahun adalah sebesar 180.40 mm. Hasil analisis debit rencana metode rasional sebesar 124.07 m3/det, sedangkan dengan metode HSS Nakayasu distribusi hujan 2 jam sebesar 207.40 m3 /det. Kedalaman aliran kritis di atas mercu bendung bangunan spillway diperkirakan setinggi 1.39 m. Elevasi muka air pada saat banjir maksimum adalah 95.66 mdpl di atas mercu bendung spillway, 88.78 - 90.11 mdpl di segmen saluran samping, 88.23 - 88.78 mdpl di segmen saluran transisi, 61.34 - 88.62 mdpl di segmen saluran peluncur, dan 66.28 mdpl di segmen akhir peredam energi., Spillway is a hydraulics building of a dam that functions during an emergency, when the water level of the maximum flood storage volume. This study aims to review the spillway building on the Randugunting Dam based on a study of the existing design of the spillway building, the design discharge of the spillway building, the critical flow depth, and the water level profile of the spillway building. The planned type of spillway building is a side spillway type with an Ogee crest type. The analysis of Banyuasin River catchment area are 17.39 km2 with the river length of 8.52 km. The result of the planned rainfall with a return period of 1000 years is 180.40 mm. The result of discharge with rational method is 124.07 m3/s, while with the Nakayasu HSS method the 2-hour rain distribution is 207.40 m3/s. The critical depth above the weir crest is estimated to be 1.39 m. The maximum water level during flooding is 95.66 masl above the spillway weir crest, 88.78 - 90.11 masl in the side channel segment, 88.23 - 88.78 masl in the transition channel segment, 61.34 - 88.62 masl in the drop channel segment, and 66.28 masl in the stilling basin segment.
Keyword: Critical flow depth, design discharge, water surface profile