Judul: Perubahan sosial di desa pertanian jawa : analisis terhadap sistem penghidupan masyarakat tani
Abstrak: Penelitian ini mencoba melihat perubahan sosial di pedesaan di Jawa dimana modernisasi pertanian dan pedesaan mendorong dengan sangat kuat perkembangan pedesaan menjadi semakin "maju". Berbagai program pengentasan kemiskinan dan peningkatan kesejahteraan masyarakat desa berdasarkan berbagai penelitian telah menyebabkan musnahnya berbagai kelembagaan lokal. Penelitian ini ingin melihat sejauh mana kelembagaan sosial asli di pedesaan mendukung sistem penghidupan masyarakat tani?
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Judul: Perubahan Sosial Masyarakat Desa Bangunjaya Sejak Tahun 1980-an
Abstrak: The result showed that modernization that began with the construction which going on in 1985 in the village of Bangunjaya an impact on people's lives. The effect is the change in aspects of community life in the village Bangunjaya the structural aspects and the cultural community and the public welfare. Changes in aspects of structural and cultural changes in the society is the system of government, the increasing diversity of livelihoods, changes in social stratification, the new social groups, as well as a shift in values and norms that exist in society. In addition, changes in the level of welfare is the difference in the condition of education, health, and income in 1980’s and the time the study was conducted according to public perception. Public attitudes towards social change mostly positive value. Public attitudes towards social change has a positive relationship with respondent characteristics including education level and income level. A negative relationship was indicated from public attitudes toward social change with respondent characteristics including age, gender, and social status.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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