Judul: Identifikasi dan Karakterisasi Fragmen DNA Genomik Kakao (Theobroma cacao L.) Hasil PCR  Menggunakan Primer Heterologous Leafy
Abstrak: Cacao, in Indonesia from the social and economically point of view, and important commoditie, through the cacao productivity is still low. The unpreddictable blooming seasons bring about the low productivity of cacao plantations.
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Judul: Amplifikasi dan Karakterisasi In Silico Daerah Pengapit Ujung 5’ Gen APETALA1 Kakao (Theobroma cacao L.)
Abstrak: Cacao is one of important export comodities in Indonesia but its productivity is still in low category. The long juvenile phase is one of the cause of low productivity of cacao plantations. The effort to increase productivity drives on cacao studies of molecular flowering gene. In Arabidopsis, APETALA1 functions in earlier developmental stages in determination of the floral meristem identity and one of flowering identity genes that determines the formation of sepal and petal tissues. The purposes of the research are to amplify 5’-flanking regions APETALA1 gene from cacao (Theobroma cacao L.) and analyse its promoter characteristic. Amplification of 5’-flanking regions APETALA1 gene using Thermal Asymmetric Interlaced-PCR (TAIL-PCR) technique shows that spesific primer and arbitrary primer pairs can amplify the flanking region. Futhermore this fragment were transformed at competent cell E. Coli XL-1 Blue by using pGEMT Easy vector. The result of bioinformatic analysis using online promoter analysis software softberry shows undiscovered TATA box/enhancer on the amplified sequence and it contains 11 motifs of plant functional promoter element. Further analysis indicated that the amplified sequence belong to TATA-less promoter group with core motif microsatellite (CG)
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Judul: Kajian Dimensi Bangunan Spillway
Abstrak: Bangunan spillway merupakan bangunan hidrolika dari suatu bendungan yang berfungsi pada saat keadaan darurat (emergency), yaitu keadaan muka air pada saat volume tampungan banjir maksimum. Penelitian ini bertujuan untuk melakukan kaji ulang bangunan spillway pada Bendungan Randugunting berdasarkan kajian terhadap desain existing bangunan spillway, debit rencana bangunan spillway, kedalaman aliran kritis, dan profil muka air bangunan spillway. Tipe bangunan spillway yang direncanakan adalah tipe side spillway dengan tipe mercu Ogee. Hasil analisis luas catchment area Sungai Banyuasin adalah 17.39 km2 dengan panjang sungai 8.52 km. Nilai curah hujan rencana dengan periode ulang 1000 tahun adalah sebesar 180.40 mm. Hasil analisis debit rencana metode rasional sebesar 124.07 m3/det, sedangkan dengan metode HSS Nakayasu distribusi hujan 2 jam sebesar 207.40 m3 /det. Kedalaman aliran kritis di atas mercu bendung bangunan spillway diperkirakan setinggi 1.39 m. Elevasi muka air pada saat banjir maksimum adalah 95.66 mdpl di atas mercu bendung spillway, 88.78 - 90.11 mdpl di segmen saluran samping, 88.23 - 88.78 mdpl di segmen saluran transisi, 61.34 - 88.62 mdpl di segmen saluran peluncur, dan 66.28 mdpl di segmen akhir peredam energi., Spillway is a hydraulics building of a dam that functions during an emergency, when the water level of the maximum flood storage volume. This study aims to review the spillway building on the Randugunting Dam based on a study of the existing design of the spillway building, the design discharge of the spillway building, the critical flow depth, and the water level profile of the spillway building. The planned type of spillway building is a side spillway type with an Ogee crest type. The analysis of Banyuasin River catchment area are 17.39 km2 with the river length of 8.52 km. The result of the planned rainfall with a return period of 1000 years is 180.40 mm. The result of discharge with rational method is 124.07 m3/s, while with the Nakayasu HSS method the 2-hour rain distribution is 207.40 m3/s. The critical depth above the weir crest is estimated to be 1.39 m. The maximum water level during flooding is 95.66 masl above the spillway weir crest, 88.78 - 90.11 masl in the side channel segment, 88.23 - 88.78 masl in the transition channel segment, 61.34 - 88.62 masl in the drop channel segment, and 66.28 masl in the stilling basin segment.
Keyword: Critical flow depth, design discharge, water surface profile