Judul: Gaya Pengasuhan, Kontrol Diri, Intensitas Bermain Game Online, dan Prokrastinasi Akademik pada Mahasiswa yang Pernah Bermain Game Online
Abstrak: Prokrastinasi akademik merupakan perilaku menunda untuk menyelesaikan tugas akademik. Penelitian ini bertujuan untuk menganalisis pengaruh karakteristik individu, karakteristik keluarga, gaya pengasuhan orang tua, kontrol diri, dan intensitas bermain game online terhadap prokrastinasi akademik mahasiswa. Penelitian ini menggunakan desain eksplanatori dengan melibatkan 220 mahasiswa S1 Institut Pertanian Bogor yang memiliki orang tua lengkap dan pernah atau sering bermain game online. Penelitian berlokasi di Institut Pertanian Bogor dan pengambilan contoh dilakukan secara purposive. Data diperoleh dengan pelaporan diri melalui kuesioner daring, selanjutnya diolah dengan analisis deskriptif, uji korelasi, dan uji regresi linear berganda. Sebagian besar ibu dan lebih dari separuh ayah menerapkan gaya pengasuhan otoritatif. Lebih dari separuh mahasiswa memiliki kontrol diri kategori rendah. Hampir separuh mahasiswa memiliki intensitas bermain game online kategori sangat tinggi dan sebagian besar mahasiswa memiliki prokrastinasi akademik kategori rendah. Hasil uji regresi (R2= .241) menunjukkan bahwa kontrol diri berpengaruh negatif signifikan terhadap prokrastinasi akademik dan intensitas bermain game online berpengaruh positif signifikan terhadap prokrastinasi akademik.
Keyword: gaya pengasuhan orang tua, intensitas bermain game online, kontrol diri, mahasiswa, prokrastinasi akademik

Judul: Influence of Parent Attachment, Self-Regulation, and Intensity of Playing Online Game toward Student Engagement
Abstrak: Keterlibatan mahasiswa dalam pembelajaran merupakan faktor keberhasilan akademik. Keterlibatan mahasiswa dalam pembelajaran mampu mengatasi kebosanan dalam belajar terlebih saat pembelajaran daring yang sedang diterapkan ataupun permasalahan lain seperti game online, permainan yang sangat disukai oleh mahasiswa. Penelitian ini bertujuan untuk menganalisis pengaruh karakteristik individu, karakteristik keluarga, kelekatan dengan orang tua, regulasi diri, dan intensitas bermain game online terhadap keterlibatan mahasiswa dalam pembelajaran. Penelitian ini menggunakan desain explanatory dengan melibatkan 220 mahasiswa sarjana. Penelitian berlokasi di Institut Pertanian Bogor dan pengambilan contoh dilakukan secara purposive. Hasil penelitian menunjukkan bahwa lebih dari separuh mahasiswa memiliki kelekatan yang sedang pada ibu dan rendah pada ayah, serta memiliki regulasi diri yang sedang. Hampir separuh mahasiswa memiliki intensitas bermain game online yang sangat tinggi dan lebih dari separuh mahasiswa memiliki keterlibatan dalam pembelajaran yang rendah. Hasil uji regresi menunjukkan besar keluarga, kelekatan dengan orang tua, dan regulasi diri berpengaruh pada keterlibatan mahasiswa dalam pembelajaran. Berdasarkan hasil penelitian keterlibatan mahasiswa dalam pembelajaran berkatagori rendah, maka peran kelekatan dengan orang tua dan regulasi disi sangat dibuutuhkan untuk meningkatkan keterlibatan mahasiswa dalam pembelajaran., Student engagement is an academic success factor. Student engagement overcome boredom at study especially at online classes or any other cases like game online, students like the most. This study aims to analyze the influence of individual characteristics, family characteristics, parent attachment, self-regulation, and the intensity of playing online game toward engagement on learning. This study used an explanatory design by involving 220 undergraduate students of IPB University. The research was located at the IPB University and the sampling was done purposively. The results showed that more than half of the students had moderate attachment to parents and had moderate self-regulation. Nearly half of students have a very high intensity of playing online games and have low student engagement on learning. The results of the influence test showed that family size, parent attachment and self-regulation had an effect on student engagement on learning. Based on the study result in the low category of student engagement, the role of parent attachment and self-regulation is needed to incrase student engagement.
Keyword: intensity of playing online games, parent attachment, self-regulation, students, student engagement

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable