Judul: Identifikasi Keragaman Gen Prolaktin (PRL) pada Ayam IPB-D1
Abstrak: Gen prolaktin pada ayam (PRL) merupakan salah satu gen yang mengontrol sifat mengeram dan produksi telur pada ayam petelur. Sifat yang banyak ditemukan pada ayam lokal yaitu sifat mengeram dan rontok bulu. Sifat ini akan menurunkan produksi telur dan mengganggu sistem reproduksi pada ayam lokal. Tujuan dari penelitian ini yaitu mengidentifikasi keragaman pada ayam IPB-D1 dengan menggunakan metode sequencing. Keragaman pada gen prolaktin dilihat pada 46 sampel DNA ayam IPB-D1. Pada penelitian ini dihitung frekuensi genotipe, frekuensi alel, dan nilai heterozigositas serta keseimbangan Hardy-Weinberg pada ayam IPB-D1. Analisis data menggunakan bantuan program aplikasi FinchTV, Bioedit, dan MEGAX. Hasil penelitian menemukan 5 SNP diantaranya g.7823 A>G, g.7835 A>G, g.7886 T>A, g.8052 T>C, g.8069 T>C. Seluruh SNPs bersifat polimorfik dan berada dalam kesetimbangan Hardy-Weinberg.
Keyword: gen prolaktin, mengeram, mutasi, produksi telur

Judul: Karakterisasi Gen Prolaktin (PRL) pada Ayam Kampung Ayam Walik Ayam Leher Gundul dan Ayam Arab
Abstrak: Indigenous and local chicken germ plasm in Indonesia have a high potency to be developed. One of the potency that can be developed is an improvement egg production. Low egg production of those chickens can be increased by selecting the in broodiness. Broodiness is controlled by prolactin gene (PRL). Since the characteristics of PRL gene of kampung, walik, leher gundul and arab are still limited refence, therefore this study aim to investigate the characteristic of PRL gene on their. Blood samples were extracted to obtain DNA. PCR then was used to amplified the fragment of PRL. A total of 20 samples successfully amplified at annealing temperature of 60 °C for 45 seconds and then directly sequenced. DNA sequencing results were analyzed using the MEGA 5 program. PRL gene in this study had similarity of 98%-99% with the PRL gene sequence data available on GenBank. This study also shows that there were 11 point mutation of PRL gene’s fragment with the type of mutations were deletions, insertions, transitions and transvertion. Those mutation types have potency as new single nucleotide polymorphisms (SNPs).
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Judul: Pembuatan Film Tipis Cu2ZnSnS4 sebagai Lapisan Penyerap Cahaya pada Sel Surya Menggunakan Metode Elektrodeposisi
Abstrak: Thin film of Cu2ZnSnS4 (CZTS) is an alternative semiconductor used as light absorber layer on solar cell. CZTS can replace silicon that commonly used as commercial semiconductor. The advantage of using CZTS as absorber layer is due to the abundance of its chemical composer on earth, and its high value of absorption coefficient. The absorber layer was made by using electrodeposition method on ITO glass substrates. The films were deposited at room temperature using potentiostatic mode with a potential of –1.05 V for 30, 45, and 60 minutes. After that, the films were annealed at two different temperatures, 180 ºC and 500 °C, for 45 minutes in N2/H2S atmosphere. Based on X-ray diffraction analysis, the polycrystalline thin film obtained fall into kesterite crystal phase. This type of crystal was obtained when the CZTS was annealed at 500 °C in H2S/N2 atmosphere. The value of optical band gap energy for the CZTS thin film was 2.2 eV with the film thickness about 1.228–6.366 μm. The sample of CZTS with electrodeposition time of 45 minutes and annealing temperature of 500 °C showed the best result and in conformity with absorber layers criteria for solar cells.
Keyword: semiconductor, ITO glass, CZTS thin film, electrodeposition