Judul: Identifikasi Keragaman Gen Hormon Pertumbuhan (GH|MspI) Pada Sapi Friesian Holstein Di Bib Lembang, BBIB Singosari, Dan BET Cipelang
Abstrak: Growth hormone (GH) is an anabolic hormone synthesized and secreted by somatotroph cells from the anterior lobe of the pituitary. The GH plays an important role in postnatal growth and development, tissue growth, lactation, reproduction, and metabolism. This study was aimed to identify polymorphism of the growth hormone gene (GH) in dairy cattle and beef cattle as a comparison. Holstein Friesian (HF) cattle for a total number of 89 heads from BIB Lembang (17), BBIB Singosari (32) and BET Cipelang (40); and four breeds of beef cattle for a total number of 37 heads from BET Cipelang were genotyped at intron 3 of the GH gene using PCR-RFLP method by MspI restriction enzyme. Genotyping the GH gene resulted in three genotypes, namely GH|MspI (+/+), (+/-), and (-/-), with two alleles, namely GH|MspI (+) and (-). Genetic polymorphism was detected in HF cattle and beef cattle, the exception was for Simental and Angus. The frequency of the GH|MspI (+) allele contrast to the GH|MspI (-) allele for HF cattle were 0.826 vs 0.174. GH|MspI (+/+) genotype had the highest frequency for HF (0,826), Simental, and Angus (1,000); but very limited for Limousin and Brahman (0,144 and 0,000). Chi-Square analysis showed that HF from the three locations were in Hardy-Weinberg equilibrium (χ2 < χ2 (0,05)), but contrast on beef cattle (χ2 > χ2 (0,05)). The value of heterozigosity expectation (He) for HF and beef cattles were estimated between 0.000-0.800. The genetic variation of the GH|MspI gene was low in most of these cattles, the exception was for Brahman.
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Judul: Identifikasi Keragaman Gen Growth Hormone Releasing Hormone (GHRH|HaeIII) Pada Sapi Friesian Holstein Di BIB Lembang, BBIB Singosari, Dan BETCipelang
Abstrak: This study was aimed to identify polymorphism of the GHRH gene in Holstein Friesian (FH) cattle from BIB Lembang, BBIB Singosari, and BET Cipelang; and beef cattle from BET Cipelang for a comparison. Blood samples used were 126 samples, consisting of 49 HF bulls from BIB Lembang (17 hds), BBIB Singosari (32 hds), and HF cows from BET Cipelang (40 hds); and a number of 37 hds of beef cattle consisting of Simmental (13 hds), Limousin (14 hds), Brahman (5 hds), and Angus (5 hds) from BET Cipelang. This study used PCR-RFLP method using restriction enzyme HaeIII. This enzyme restricted the GHRH gene sequences at the bases GG | CC at the positions of 118, 312, and 406 bp resulting four fragments with the lengths of 118, 194, 94, and 45 bp respectively. Genotyping GHRH gene produced two alleles, namely A and B alleles. These alleles produced three genotypes, namely the genotypes of AA (312, 94, and 45 bp), AB (312, 194, 118, 94, and 45 bp), and BB (194, 118, 94, and 45 bp). Frequency of the B allele was higher than the A allele. Frequencies of the A and B alleles in HF were in BIB Lembang 0,235 and 0,765, BBIB Singosari 0,234 and 0,766, and BET Cipelang 0,225 and 0,775. Chi-square analysis showed that all of HF and beef cattles were in Hardy-Weinberg equilibrium (χ2 < χ2 (0.05)), the exception in HF cattles in BIB Lembang, Brahman and Angus cattles in BET Cipelang. The highest heterozigosity values in HF was found in BIB Lembang (0,471), while the lowest was in BBIB Singosari (0,344). Heterozygosity values in the HF were lower than those of Simmental and Limousin beef cattle. GHRH gene in HF and beef cattle were polimorfic, the exception in Brahman cattle as possesing only the B allele.
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Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4