Judul: Faktor-Faktor yang Berhubungan dengan Gangguan Makan pada Remaja Putri di Model Agency Tasikmalaya
Abstrak: Objective of this research was to analyze factors associated to eating disorders in adolescent girls. Study design used in this research was cross sectional study. The number of samples was 61 adolescent girl aged 12-21 years in the Tasikmalaya model agency. The results showed adolescent girls with eating disorders are 46 girls (75,4%) and 15 girls (24,6%) classified as normal. The type of eating disorder are 38 girls (62,3%) eating disorder not otherwise specified, five girls (8,2%) binge eating disorder, two girls (3,3%) bulimia nervosa, and one girl (1,6%) anorexia nervosa. Dietary history (OR: 3,71; 95% CI: 1,03- 13,76) and internet access (OR: 4,44; 95% CI: 1,16-17,02) associated significantly (p<0,05) with eating disorder. However, eating disorder is not associated significantly with body perceptions, magazines/tabloids access, watching television, criticing by friend, boyfriend, and family, as well as family dietery habits.
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Judul: Faktor-Faktor yang Berhubungan dengan Gangguan Makan pada Remaja Perempuan di Modeling School
Abstrak: The objective research was to analyze the factors associated with eating disorders in adolescent girls. Research design used in this was cross sectional study. The number of samples were are 50 adolescent girls from Modeling school aged 15-22. There are 25 adolescent girls with eating disorders and 25 people (50.0%) classified as normal. This type of eating disorders are as follow: 15 girls with no specified eating disorder, six girls with binge eating disorder, three girls with anorexia nervosa, and one person with bulimia nervosa. There is a significant association between eating disorders and the self-confidence (OR=6.47; 95%CI=1.230-34.012; p=0.017), body image distortion (OR= 7.58; 95%CI=0.839-68.461; p=0.04), the peer criticism (OR=4.89; 95%CI=1.150- 20.790; p=0.024) and mass media (OR=6.47; 95%CI=1.230-34.012; p=0.017) (p<0.05).
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Judul: Isolasi Konstruksi dan Rekombinasi Gen Pengkode Reverse Transcriptase asal Simian Retrovirus-2 (SRV-2)
Abstrak: The availability of reverse transcriptase enzym as a valuable component for Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique has been providing by foreign companies. Therefore, it needs more cost and time to provide it. This study is an early step to produce in house reverse transcriptase rekombinant enzym from SRV-2. The purpose of this study was isolated, constructed, and rekombined SRV-2 reverse transcriptase gene to entry clone and destination vector. Isolation was done by PCR amplification technique using three pairs of primers, which are RT I (3282-3683), RTII (3261-3900), and RTIII (3169-4133). Construction and recombination in this study used Gateway method from Invitrogen. SRV-2 reverse transcriptase gene was isolated in this study with A-549 cell inoculated SRV-2 as source of virus. This gene amplify by PCR technique and resulted specific band for RTI 401 bp, RTII 639 bp, and RTIII 964 bp. Analysis of pENTR/SD/TOPO and pDEST17 indicated SRV-2 reverse transcriptase gene code has already inserted to this plasmid. This result indicated SRV-2 reverse transcriptase gene have isolated, constructed, and recombined to entry clone and destination vector.
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