Judul: Social economics analysis of benefit marine protected area in Kepulauan Seribu, DKI Jakarta
Abstrak: This research is aimed to determine weather there are social and economic benefits that can be derived from marine protected area of Taman Nasional Laut Kepulauan Seribu. Especially this study was aimed to determine the social and economic consequence of establishment of MPA in Kepulauan Seribu. The study was time series analysis to asses tren of supporting facilities in Taman Nasional Laut. Result from this time series were then used to asses sustainability status of TNKL by means of Marine Protected Area Evaluation Model (MPAEM). A multicriteria analysis was used to evaluate socio-economic perceptions of people living at TNKL. Finally an economic valuation technique was employed to asses the monetary benefits or losses associated with the establishment of MPA.
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Judul: Economic Benefit Analysis of Fishery Resource at Marine Protected Area of Karimunjawa National Park.
Abstrak: Marine ecosytem has many benefits that provide an important economic justification for establishing Marine Protected Area (MPA), but these are often not fully understood by local community surrounding and stakeholders. This research was aimed to distinguish whether the establishment of Taman Nasional Karimunjawa (TNKj) as a MPA has contributed economic benefits of fishery resource to the local community as well as the regional economy. The change of benefit or loss rate was calculated using productivity approach of Economic Valuation Method and an iconic simulation. Location Quotient (LQ) analysis was applied to see the contribution of fishery sub sector to regional economic of Jepara. To know the perception of fishermen, Descriptive method was used. The Result showed that at present the economic benefit of fishery wasn’t quite significant, this probably corresponded to the increase of destructive fishing activities prior to the establishment of TNKj in 1999. The contribution of fishery sub-sector to Gross Domestic Product (GDP) of Jepara in 1999-2004 declined. However the datas indicated that the benefit would be obtained in afew years, as sea environment degradation declined and conservation management became more effective. As MPA, sustainable aspect is the most important thing, therefore the economic development should be in line with the ecology. It needs good management and coordination among all stakeholders as well as wider involvement of local community. The concept of collaborative management is proper to examine for advanced research.
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Judul: Construction and Subcloning of the GOx-Xho Gene Encoding Glucose Oksidase Enzyme into the Plasmid pPICZα
Abstrak: Glukosa oksidase (GOx; EC 1.1.3.4) adalah enzim yang mengoksidasi Dglukosa menjadi D-glukono-d-lakton dan menghasilkan hidrogen peroksida. Aktivitas enzim tersebut pada organisme asalnya, yaitu Aspergillus niger IPBCC 08.610, diketahui lebih tinggi secara intraseluler dibanding ektraselular sehingga upaya memproduksi GOx secara masif kurang efisien. Salah satu solusi dari permasalahan tersebut adalah dengan metode alternatif, yaitu produksi enzim GOx secara rekombinan. Penelitian ini bertujuan mengonstruksi ulang fragmen gen GOx dengan menambah situs restriksi XhoI menjadi GOx-Xho, mensubkloning gen tersebut ke dalam plasmid pPICZα, dan mentransformasi plasmid rekombinan ke dalam sel Escherichia coli DH5α. Konstruksi gen GOx dilakukan menggunakan metode PCR dalam dua tahap. Ligasi gen GOx dengan plasmid pPICZα dilakukan dengan dua perlakuan, yaitu dengan rasio 1:1 dan 1:3 antara plasmid dan gen. Hasil penelitian menunjukkan perbandingan plasmid dan gen yang optimal dalam subkloning adalah 1:3. Hasil subkloning berhasil ditransformasi ke dalam sel E. coli DH5α dan sel pembawa plasmid rekombinan pPICZα-GOx-Xho berhasil diperoleh.
Keyword: glucose oxidase enzyme, recombinant plasmid, reconstruction gene, subcloning gene