Judul: Eco-Design of Merdeka Park as an Alternative to the Development of Urban Green Open Space
Abstrak: Taman Merdeka berperan penting secara sosial dan ekologis di tengah padatnya Kecamatan Sukmajaya. Taman ini membutuhkan desain yang mampu menyediakan ruang yang indah dan memenuhi kebutuhan pengunjung tanpa mengesampingkan fungsi ekologisnya. Dengan demikian, desain Taman Merdeka perlu dilakukan dengan pendekatan desain ekologis yang mengurangi dampak negatif terhadap lingkungan. Penelitian ini bertujuan mengidentifikasi dan menganalisis kondisi existing tapak, menyusun konsep desain ekologis Taman Merdeka, dan mendesain secara ekologis Taman Merdeka Depok yang indah, fungsional, dan ramah lingkungan. Penelitian ini dilaksanakan menggunakan metode deskriptif melalui empat tahap yang mencakup persiapan, inventarisasi, analisis dan sintesis, dan desain dengan pendekatan fisik dan biofisik, sosial, dan desain ekologis. Konsep dasar dari desain ekologis Taman Merdeka adalah Urban Eco-Excursion yang berarti sebuah ruang terbuka hijau yang mampu mengintegrasikan berbagai kegiatan, untuk berbagai kelompok, dengan pendekatan yang ramah lingkungan. Konsep desain dikembangkan dari motif Batik Depok. Desain ekologis diterapkan melalui penggunaan green infrastructure untuk stormwater infiltration and cleaning, konservasi vegetasi eksisting, upcycling center untuk pengelolaan sampah, dan penggunaan bahan yang ramah lingkungan. Desain taman mencakup bioswales, stormwater wet pond, lapangan-lapangan olahraga, playground, plaza, area pujasera, upcycling center, welcome area, area duduk, elevated walkway, amphitheatre, lawn, promenade, dan toilet., Merdeka Park plays important social and ecological roles in the midst of the very dense Sukmajaya District. This park needs a design that is able to provide an aesthetically pleasing space and meet the needs of visitors without neglecting ecological functions. Thus, the design of Merdeka Park needs to be done with an ecological design approach that minimizes the impacts that may damage the environment. This study aims to identify and analyze the existing site conditions, create an ecological design concept of Merdeka Park, and ecologically design Merdeka Park that is beautiful, functional, and environmentally friendly. This study was conducted using descriptive method through four stages which are preparation, inventory, analysis and synthesis, and design with a physical and biophysical, social, and ecological approaches. The basic concept for the eco-design of Merdeka Park is Urban Eco-Excursion which means a green open space that integrates various activities for various groups with an environmentally friendly approach. The design concept is inspired by Depok's Batik. Eco-design is implemented through the use of green infrastructure for stormwater infiltration and cleaning, conservation of existing vegetation, upcycling center for waste management, and environmentally-friendly materials. The design of the park include bioswales, stormwater wet pond, sports fields, playground, plaza, food court, upcycling center, welcome area, seating areas, elevated walkway, amphitheatre, lawn, promenade, and toilet.
Keyword: green infrastructure, recycling, upcycling, urban park

Judul: Design of Amri Yahya Park Kayuagung Ogan Komering Ilir South Sumatera Based on Ecodesign Concept
Abstrak: Taman Amri Yahya sebagai taman kota memiliki lokasi yang strategis dan potensial tetapi belum memiliki pola desain dan konsep fungsional yang jelas. Permasalahan yang ditemukan yakni penataan tanaman yang kurang tertata, kurangnya pemeliharaan softscape dan hardscape, dan pola desain lanskap yang kurang jelas sehingga kualitas visual lanskap menurun. Penelitian ini bertujuan mengidentifikasi dan menganalisis kualitas desain dan fungsi berdasarkan potensi dan kendala pada Taman Amri Yahya, menganalisis persepsi dan preferensi masyarakat terhadap kondisi Taman Amri Yahya sebagai sumber acuan pada proses desain, dan membuat usulan desain Taman Amri Yahya berbasis konsep Eco design. Penelitian ini menggunakan metode deskriptif kualitatif dan kuantitatif dengan mengambil data persepsi dan preferensi Masyarakat untuk mendapatkan solusi desain. Hasil dari penelitian ini menunjukkan bahwa kualitas desain saat ini kurang baik dengan kerusakan pada fasilitas dan utilitas sehingga menurnkan estetika. Persepsi masyarakat menunjukkan bahwa taman terasa nyaman saat pagi dan sore hari namun tidak didukung denga kelayakan fasilitas dan utilitas. Preferensi masyarakat berupa adanya fasilitas pendukung seperti bench, foodcourt, toilet umum, amphitheater, dan playground serta penggunaaan desain modern yang geometrik dan organik. Konsep ecodesign diimplementasikan sebagai konsep dasar pada proses desain. Konsep desain Taman Amri Yahya mengusung perahu kajang dan sungai yang menggambarkan budaya dan ciri khas identitas Kabupaten OKI., As a city park, Amri Yahya Park has a strategic and potential location but still needs a clear design pattern and functional concept. The problems found are the arrangement of less organized plants, lack of softscape and hardscape maintenance, and unclear landscape design patterns so the visual quality of the landscape decreases. This study aims to identify and analyze design quality and function based on the potential and constraints of Amri Yahya Park, analyze community perceptions and preferences on the condition of Amri Yahya Park as a reference source in the design process, and make suggestions for designing Amri Yahya Park based on the eco-design concept. The method used in this research is a descriptive qualitative and quantitative methods by taking data perceptions and community preferences to obtain design solutions. The results of this study indicate that the current design quality is not good with damage to facilities and utilities thereby reducing aesthetics. Community perceptions indicate that the park feels comfortable in the morning and evening but it is not supported by proper facilities and utilities. Community preferences include supporting facilities such as benches, food courts, public toilets, amphitheater, and playgrounds as well as the use of geometric and organic modern designs. The eco-design concept is implemented as a basic concept in the design process. The design concept for Amri Yahya Park is perahu kajang and river flow which depicts the culture and identity characteristics of OKI Regency.
Keyword: Ecological, Green Infrastructure, Landscape Design, Public Park, Urban Green Space

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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