Judul: African Swine Fever Virus Detection Using Gel Based Polymerase Chain Reaction
Abstrak: African Swine Fever (ASF) diyakini sebagai salah satu penyakit menular babi yang paling berbahaya dan menyebabkan kematian yang tinggi pada babi. Gejala klinis maupun pemeriksaan postmortem ASF sulit dibedakan dari Classical Swine Fever (CSF) dan penyakit hemoragik lainnya. Karenanya uji laboratorium penting dilakukan untuk mengetahui langkah yang tepat dalam pengobatan dan pengendaliannya. Penelitian bertujuan mendeteksi virus ASF di organ (paru-paru, hati, dan ginjal) babi menggunakan teknik Polymerase Chain Reaction (PCR) berbasis gel dengan primer P72-U dan P72D sebagai langkah peneguhan diagnosis penyakit. Metode penelitian ini terdiri dari beberapa tahapan seperti preparasi sampel, isolasi DNA virus, uji PCR, elektroforesis, dan visualisasi hasil. Hasil menunjukkan reaksi positif pada semua sampel organ tetapi pita amplifikasi sampel ginjal terlihat lebih tipis yang kemungkinan disebabkan oleh konsentrasi DNA virus yang relatif lebih sedikit pada ginjal. Penelitian lebih lanjut diperlukan untuk mengetahui jumlah konsentrasi virus dan kepastian penyebab tipisnya pita.
Keyword: African swine fever, pig, PCR

Judul: Pathological Overview of African Swine Fever (ASF) in Pigs (Sus scrofa domesticus)
Abstrak: Babi domestik merupakan salah satu ternak potensial yang semakin meningkat produksinya. Maraknya kasus African Swine Fever (ASF) mengakibatkan berbagai kerugian antara lain kerugian sosial, ekonomi, bahkan lingkungan. Berdasarkan permasalahan tersebut, perlu diketahui lebih lanjut gambaran patologi penyakit ASF. Studi kasus ini bertujuan mempelajari gambaran patologi ASF pada babi (Sus scrofa domesticus). Penelitian dilakukan dengan nekropsi pada babi, beberapa organ dan bagian diamati. Kemudian diambil sampel organ sebagai preparat histopatologi dengan pewarnaan hematoksilin eosin (HE). Perubahan dalam patologi anatomi dan histopatologi kedua ekor babi mengindikasikan kejadian ASF. Perubahan patologi anatomi seperti hemoragi pada beberapa organ, meliputi otak, kulit, kelenjar pertahanan, dan limpa sebagai organ target utama. Data ditunjang dengan pemeriksaan melalui pewarnaan HE yang menunjukkan lesio-lesio ASF. Bukti kuat ditandai heemorrhagic splenitis dan infiltrasi sel radang pada limpa sebagai tanda patognomonik ASF. Berdasarkan hasil kedua pemeriksaan tersebut, babi terinfeksi ASF dengan tipe akut., Domestic pigs are one of the potential livestock whose production is increasing. The rise of cases of african swine fever (ASF) has resulted in various losses, incluiding social, economic and even environmental losses. Based on these problems, it is necessary to know more about the pathological features of ASF disease. This case study aims to study the pathological overview of ASF in pigs (Sus scrofa domesticus). The research was carried out by necropsy on pigs, observing several parts and organs. Then organ samples were taken as histopathological preparations stained with hematoxylin and eosin (HE). Changes in the anatomical pathology and histopathology of the two pigs indicate the occurrence of ASF. Anatomical pathological changes such as hemorrhage in several organs, including the brain, skin, defense glands, and spleen as the main target organs. The data was supported by examination through HE staining which showed ASF lesions. Strong evidence also supported by hemorrhagic splenitis and infiltration of inflammatory cell of the spleen as pathognomonic signs of ASF. Based on the results of the two examinations, pigs were infected with ASF of the acute type.
Keyword: akut, ASF, histopatologi, patognomonik, patologi anatomi

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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