Judul: Analisis hubungan kekerabatan manggis (Garcinia mangostana L.) menggunakan penanda morfologi dan molekuler (ISSR) terhadap kerabat dekatnya
Abstrak: Mangosteen and its relatives of the genus Garcinia L. belong to the family Guttiferae which contains about 35 genera and up to 800 species. Guttifferae diversity was found across the Indonesian archipelago. In order to elucidate genetic diversity of mangosteen and its relatives, morphological and molecular analysis was conducted. Analysis of relationship through morphological marker and molecular ISSR (Inter Simple Sequence Repeat) between 19 accessions of G. mangostana and their close relatives revealed the phylogenetic relationship. The diversity based on 83 morphological characters, obtained 83 (100%) polymorphic loci Analysis of similarity resulted in 4 groups with distance of similarity coefficient 0.45-1.00. The diversity analysis using 12 ISSR primers, generated 130 loci in which 129 (99.23%) polymorphic loci and 1 (0.77%) monomorphic loci with 0.40-1.00 similarity coefficient, formed 3 groups. It is note worthy that epidermis cells pattern surround stomata cell of G. mangostana is similar same as G. malaccensis and G. celebica. It is concluded that G. celebica has a close relationship with G. mangostana and G. malaccensis.
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Judul: Analisis konsistensi pola genetik empat generasi manggis (garcinia mangostana l.) berdasarkan marka ISSR
Abstrak: Mangosteen reproduces trough apomixes mechanism, from which the seed develops without fertilization. Mangosteen is an obligate apomicts and it is believed that all of its progenies may have the same genotype as their mother plant. However it was found that genetic variations occurred among mangosteen accessions. This research is aimed to study pattern of morphology variations, genetic consistency among generations and genetic variations in single plant. The study was carried out at Center for Tropical Fruit Studies (PKBT) during February – September 2009. The plant materials used four generations (P1, P2, P3, and P4) of mangosteen were taken from Wanayasa, Purwakarta according to tree height and sector (north, east, west, and west). There are three analysis: morphology, molecular by ISSR, and data. Genetic observations were conducted by using Inter Simple Sequence Repeat (ISSR) technique. Ten primers were used to amplify the genomic DNA of mangosteen. The levels of polymorphism as revealed ISSR were 70.21%. It was found that genetic and morphology variations occurred among tree of different age. Morphology variations was bigger than genetic variations among mother plant. Genetic variation also was occurred within single plant, among progenies, and between progeni and mother plant. This information may be useful to determine of optimum age of mangosteen for becoming mother plant.
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Judul: Pemanasan Sekunder Reaksi Fusi Plasma dengan Menggunakan Radio Frequency
Abstrak: Plasma merupakan bentuk ke-empat dari materi setelah padat, cair dan gas yang terbentuk dari proses ionisasi gas. Plasma dapat digunakan dalam reaksi fusi, yaitu reaksi penggabungan dua inti atom ringan menjadi inti atom yang lebih berat sambil melepaskan energi. Untuk mendapatkan energi dari reaksi fusi, inti yang akan direaksikan harus dipanaskan pada temperatur yang sangat ekstrem tingginya. Salah satu cara agar dapat mencapai kondisi ini adalah memberikan pemanasan tambahan atau pemanasan sekunder kepada plasma dalam reaksi fusi tersebut. Nilai daya serap rata-rata ion Deuterium yang sesuai dengan selang nilai daya dari data ITER adalah daya yang menggunakan frekuensi 50 MHz sampai dengan 60 MHz. Dengan menggunakan frekuensi 60 Hz diperoleh nilai daya rata-rata yang diserap sebesar 1,235.10-12 W.
Keyword: gelombang radio, pemanasan sekunder, plasma, reaksi fusi