Judul: Penggandaan Kromosom Jati (Tectona grandis L.) dengan Oryzalin dalam Kultur In Vitro dan Pendugaan Tingkat Ploidi
Abstrak: Teak (Tectona grandis L.) is one of the forestry plants that have a high economic value and an important role in carbon absorbing. Chromosome doubling in teak was intended to produce a better plant both morphologically and it’s wood quality. Antimicrotubule, such as oryzalin treatment is one of the chromosome doubling inducing method. This research was conducted to get the most effective concentration and treatment period to induce the chromosome doubling of teak through in vitro culture. It’s effectivity was measured by the changes of chloroplast number in the stomatal guard cell and stomatal density, also supported by the lowest lethal effect that was measured by it’s survival percentation. The most effective oryzalin treatment to induce chromosome doubling of teak from diploids into tetraploids was estimated at 5 μM concentration with 3 days treatment period. The survival at this treatment reached 70% of the treated plants. In which about 60% of the treated plants had a doubled chloroplast number in their guard cells from average 6,0-11,9 into 12,0-23,9 chloroplast per stoma along with the decreased of stomatal density.
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Judul: Pembentukan Tanaman Haploid Ganda dengan Perlakuan Oryzalin Secara In Vitro pada Biji Jagung (Zea mays) Diduga Haploid
Abstrak: BC2 maize purple kernels estimated haploid were resulted from a cross between Bisma variety with haploid inducer line (3490) by backcross. Chromosome doubling to these kernels was done to produce doubled haploid plants (pure lines). This study was conducted to get an in vitro diploidisation method by oryzalin treatment as a chromosome doubling agent, with concentration of 0, 5, 10, and 15 mM for 24 hours on seeds and root of seedlings. Chromosome analysis on the control of purple kernels showed 66.7% of the seeds was haploid and 33.3% was diploid. Oryzalin treatment affected in doubling the chromosome number from haploid to doubled haploid or from diploid to tetraploid. The higher concentration of oryzalin increased the opportunity of chromosome doubling, but increased death rate. Combination of treatment that is most efficient was 5 mM oryzalin on seedling roots that produced the highest number of doubled haploid plants was found by 71.4% with a low mortality rate by 30%. Increasing of the chromosome number caused the growth of the longer canopy length and more number of leaves and the lower stomata density.
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Judul: Infeksi Virus Parainfluenza Pada Anjing
Abstrak: Virus parainfluenza anjing termasuk famili Paramyxoviridae, berbentuk pleomorf dengan ukuran bervariasi (bergaris tengah antara 100-500 nm), berpembungkus peka ether, berisi genom RNA dan secara serologis mempunyai kesamaan dengan virus SV-5 kera; dikenak sebagai salah satu kausa penting dari sindrom "kennel cough". Infeksinya dapat mengenai anjing semua umur yang belum pernah terinfeksi atau mendapatkan vaksinasi mengakibatkan gejala klinis ringan yang terbatas pada traktus respirasi atu infeksi yang secara klinis tidak memperlihatkan gejala nyata. Gejala klinis yang nampak setelah masa inkubasi (1-5 hari) terlewati berupa batuk yang tidak berat selama beberapa hari, berbagai tingkat tonsilitis dan pharyngitis dan kadang-kadang ditemukan kenaikan temperatur rektal antara 1-2 derajat C dari temperatur normal rat-rata. Anjing terinfeksi mengandung virus pada traktus respirasi bagian atas dan bawah serta limfonoduli sampai hari ke 9 setelah infeksi. Penyebaran virus antar anjing terjadi secara aerogen.
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