Judul: Konsumsi Ikan, Status Gizi dan Prestasi Belajar Siswa SekolahDasar Kelas IV di Daerah Pantai dan Daerah Non Pantai
Abstrak: The general objective of this study was to analyze fish consumption, nutritional status and learning achievement of 4th grade elementary school children in coastal and non coastal areas. The research was conducted by using Cross Sectional Study design and implemented in September to Desember 2011 in Tanjung Pasir and Ciriung III elementary schools. Samples were determined by purposive sampling, 30 students from coastal and non coastal areas were chosen. Amount of fish consumption in coastal areas (57.6 g) was higher than non coastal areas (32.6 g). Most student in coastal areas (43.3%) and in non coastal areas (76.7%) were caregorized as normal based on BMI for age. There was no significant association between fish consumption with nutritional status. There was a significant association between nutritional status with learning achievement (height for age r=0.320, p<0.05 and BMI for age r=0.255, p<0.05). There was a significant association between fish consumption with learning achievement (p<0.05).
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Judul: Konsumsi Ikan dan Kaitannya dengan Status Gizi, Tingkat Kecerdasan, dan Prestasi Belajar Siswa Sekolah Dasar di Kabupaten Karawang
Abstrak: This study aimed to determine the relationship between fish consumption and nutritional status, intelligence level, and academic achievement among elementary school students in Karawang district. A Cross-sectional design was used in this study. Samples were 148 students of V and VI grades. The result showed that fish consumed by most samples are shrimp (88.5%), shellfish (81.8%), crab (80.4%), sardines (70.9%), and snapper fish (60.1%). Samples’s average fish consumption and protein intake from fish were 45 g/day and 10.9 g/day, respectively. A total of 60.9% and 70.9% samples had normal nutritional status by BMI/A and H/A. Intelligence Level (IQ) and academic achievement of samples were generally categorized as good. Spearman correlation test showed that there was no significant correlation between fish consumption (frequency, total, protein intake) and nutritional status, IQ level, and academic achievement (p > 0.05), except protein intake with H/A nutritional status (p = 0.020). Nutritional status by BMI/A was not correlated significantly with IQ level and achievement (p > 0.05). Whereas H/A nutritional status had significant correlation with IQ level on 90% confidence level (p = 0.076) and academic achievement (p = 0.029).
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Judul: Isolasi Konstruksi dan Rekombinasi Gen Pengkode Reverse Transcriptase asal Simian Retrovirus-2 (SRV-2)
Abstrak: The availability of reverse transcriptase enzym as a valuable component for Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique has been providing by foreign companies. Therefore, it needs more cost and time to provide it. This study is an early step to produce in house reverse transcriptase rekombinant enzym from SRV-2. The purpose of this study was isolated, constructed, and rekombined SRV-2 reverse transcriptase gene to entry clone and destination vector. Isolation was done by PCR amplification technique using three pairs of primers, which are RT I (3282-3683), RTII (3261-3900), and RTIII (3169-4133). Construction and recombination in this study used Gateway method from Invitrogen. SRV-2 reverse transcriptase gene was isolated in this study with A-549 cell inoculated SRV-2 as source of virus. This gene amplify by PCR technique and resulted specific band for RTI 401 bp, RTII 639 bp, and RTIII 964 bp. Analysis of pENTR/SD/TOPO and pDEST17 indicated SRV-2 reverse transcriptase gene code has already inserted to this plasmid. This result indicated SRV-2 reverse transcriptase gene have isolated, constructed, and recombined to entry clone and destination vector.
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