Judul: Pengembangan Metode Isolasi Total RNA, mRNA dan Sintesis cDNA Sengon (Falcataria moluccana L. Nielsen) yang Sehat dan Terserang Hama Boktor (Xystrocera Festiva).
Abstrak: Sengon (Falcataria moluccana L. Nielsen) has a natural self-defense mechanism towards pest infection by synthesizing certain macromolecul such as tripsin inhibitor and alfa-amilase inhibitor. Characterization of those genes which produce the inhibitor need to be done on resistant trees and the vulnerable ones, to define the differences so that can be used for tree improvement. This research aim to develop an appropriate isolation methods of total RNA, mRNA and cDNA of plants that have a inhibitor enzymes such as sengon. The materials used in this research were cambium and timber tissue from resistant and vulnerable tree. Total RNA was isolated using Total RNA Mini Kit by Geneaid, mRNA was isolated using PolyATract mRNA Isolation System III with Magnetic Stand by Promega and cDNA synthesize was conducted using cDNA Substraction Kit by Clontech and RevertAid RT Transcription Kit by Thermo. The highest concentration of total RNA isolation were 65,60ng/μl from modification methods, and the best mRNA yields was resulted by modification methods. The highest concentration for cDNA synthesize were 2296,17ng/μl from modification methods.
Keyword: sengon, RNA and mRNA isolation, cDNA synthesize

Judul: Analisis Transkriptomik Pohon Sengon (Falcataria moluccana Miq.) Rentan dan Resisten terhadap Hama Boktor (Xystrocera festiva Pascoe).
Abstrak: Sengon (Falcataria moluccana Miq.) plantations in monoculture system has increases boktor (Xystocera festiva Pascoe) pest population and distribution, however there is no effective and efficient control method yet. This study aims to analyze sengon transcriptomic data and identify expressed genes involved in sengon resistance to boktor pests, also other genes related to abiotic and biotic stresses. Unpublishe de novo transcriptomic data was acquired using the RNAseq and deposited in the DDBJ repository with accession number DRA008389. DEG analysis was perfomed using R program with edgeR package. The results of gene screening were then annotated using genes analog with the Swissprot database. DEG analysis produced 6859 upregulated contigs, genes in resistant tree were Heat shock cognate 70 kDA protein, Trypsin inhibitor, and Chalcone synthase. Based on previous research, trypsin inhibitor is a defensive mechanism of sengon against boktor pests, while other genes are known to be involved in the abiotic and biotic stress response.
Keyword: de novo transcriptomic, Falcataria moluccana Miq, trypsin inhibitor, Xystocera festiva Pascoe

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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