Judul: Isolasi Promotor Gen LFY pada Tanaman Theobroma cacao L. dengan Teknik Genome WalkingTM
Abstrak: Cacao is one of the main agricultural commodities in Indonesia. Unfortunately, there are many obstacles faced by Indonesian cacao farmers, which results in the low cacao productivity. A major obstacle is the cherelle wilt disease. This disease is affected by the central flowering gene (gene LFY), whose expression level is regulated by its promoter. The objective of this research is to isolate the promoter of gene LFY, to then be cloned into its cloning and expression vectors. The promoter isolation was done from a young cacao leaf. The DNA in the leaf was extracted first, and then the promoter was isolated using Genome WalkingTM technique. Subsequently, the sequencing method and Softberry software were utilized to determine the promoter’s nucleotides. The result, which was then confirmed by TATA box, proved the existence of gene LFY promoter fragment. A fragment of 1650 bp size was then cloned into cloning and expression vectors: pGEM-T Easy and pCambia 1303. Next, the plasmid of the recombinant DNA was transformed into Agrobacterium tumefaciens using the AGLO strain. The conclusion of this research is that the gene LFY promoter of Theobroma cacao L. can be isolated using the Genome WalkingTM method, enabling more information on the occurence of cherelle wilt on cacao plants.
Keyword: 

Judul: Isolasi dan Karakterisasi Fragmen Gen LEAFY dari Bantalan Bunga Kakao
Abstrak: Cocoa plant productivity in Indonesia is still low. Information and knowledge at the molecular level about the process of development of regenerative organs of cocoa can be an effective solution to problems associated with the regenerative low productivity of cocoa plants.LEAFY gen is key gene in the shoot meristem transition to flower one. The purposes of the research were to isolate LEAFY gene from cacao’s flower cushion and analyse its expresion in several cacao’s tissues. Isolation of LEAFY gene using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) technique showed that three spesific primer pairs designed based conserved area can amplify the target gene. Futhermore this fragment were inserted into pGEM-T Easy vector then transformed into competent cell E. Coli XL-1 Blue. The result of bioinformatic analysis showed that the fragment of target gene has a high homology to LEAFY gene of other plants in gene bank’s database. Characterization of target gene was initiated with RNA isolation phase from several cacao’s tissues then those were amplified with RT-PCR technique. Amplification at 62 ºC of annealing temperature for 33 cycles was successful to show the existence of LEAFY gene in several cacao’s tissues isolated.
Keyword: 

Judul: Studi tentang pengaruh panjang saluran, debit, dan dimensi saluran terhadap kehilangan air pada saluran
Abstrak: Penelitian ini merupakan suatu studi tentang kehilangan air pada saluran untuk berbagai panjang saluran, debit air yang diberikan, dan dimensi saluran. Dari data-data, yang diperoleh dalam penelitian diharapkan dapat dibuat suatu alat untuk menduga kehilangan air pada saluran. Saluran-saluran yang diteliti mempunyai kemiringan dasar 0.00021 dan "side slope" 1:1.5. Kehilangan air diukur pada panjang saluran 150 m, 300 m, dan 450 m. Debit air yang diberikan pada saluran yang diteliti adalah 74.4-104.9 liter/detik dan 172.3 192.6 liter/detik pada saluran sekunder Tinggarjambe yang mempunyai lebar dasar saluran 65 cm, 191.0 267.0 liter/detik dan 395.3438.3 liter/detik pada saluran sekunder Doplang (kode ruas BDp4 BDp5) yang mempunyai lebar dasar saluran 105 cm, dan debit 405.0-475.0 liter/detik dan 666.5 710.0 liter/detik pada saluran sekunder Doplang (kode ruas BDp2 BDp3) yang mempunyai lebar dasar 130 cm. Kehilangan air pada saluran ada kecenderungan untuk meningkat dengan semakin panjang saluran, semakin kecil debit yang diberikan, dan semakin besar dimensi saluran. ...
Keyword: