Judul: Transplantasi sel testikular ikan gurame pada ikan nila
Abstrak: One of the constraints in the cultivation of giant gouramy is the lack of fry availability, necessitating the development of fry production engineering technology to address these issues. Engineering technology of fry production through the surrogate broodstock generated by testicular cells transplantation technique has been developed in salmonid. Testicular cells transplantation technology requires a suitable recipient that can accept and support the development of spermatogonia donor cell. This study examined the potential of Nile tilapia as a recipient for donor giant gouramy cells by observing its colonization in the gonads of Nile tilapia. Fish donor of 598,82 grams body weight with Gonado Somatic Index (GSI) of 0,013% and spermatogonial cells percentage of 28,22% was used. Testicular cells in amount of 20.000 cells/0,5 μl PBS were injected into peritoneal cavity of 4-day-old tilapia larvae. The success of transplantation and donor cell colonization were analyzed using PCR method with DNA template that have been extracted from tilapia larvae of 1 day post injection and the gonad of 2 months old fish. PCR was performed using a set of specific primer for the giant gouramy growth hormone gene and tilapia β-actin as an internal control of DNA loading. PCR results showed that the transplanted tilapia has a DNA band in the same size with giant gouramy, and not in untransplanted control. The success rate of transplantation in larvae was 100% (5/5) and the percentage of individual recipients containing donor cells in their gonad was 60% (3/5). This indicates that donor cells from giant gouramy was successfully injected and can be colonized in the gonads of Nile tilapia, so the Nile tilapia potentially be used as recipient for giant gouramy testicular cells transplantation. Further analysis needs to be conducted to observe the ability of spermatogonial cells develop into sperm and eggs in the gonad of tilapia recipient.
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Judul: Transplantasi sel testikular ikan gurame Osphronemus gouramy pada ikan nila Oreochromis niloticus umur 1-4 hari
Abstrak: Giant gouramy is one of the targeted farmed species to increase its production level in the Indonesian Ministry of Maritime Affairs and Fisheries Program 2010-2014. Its production seemed slow because of the relatively long reproductive cycle. Transplantation of giant gouramy testicular cells to tilapia can be done in the hope that the surrogate broodstock (tilapia) will be able to give birth giant gouramy so the production cycle can be accelerated. In this research, transplantation of giant gouramy testicular cell (donor) in the peritoneal cavity of newly hatched tilapia (recipient) was performed. Giant gouramy testicular cells that contain spermatogonial cells obtained by dissociation of the gonad using PBS with 0.5% trypsin and then injected into tilapia with different ages. The results showed that SR (survival rate) of 1-2 days old injected larvae (A; 89.34%) were lower compared to that of 3-4 days old (B; 98.96%). Furthermore, the success rate of donor cells colonization in the recipient gonad at 2 months after injection in treatment A (6 of 6 recipients; 100%) was higher than that of treatment B (3 of 4 recipients; 75%). The results of this study indicated that transplantation using 1-2 days old larvae gave highest colonization and comparable level of SR. Thus, this technique has the potential both to manipulate gamet production of economically important fish species that relatively difficult to reproduction (commercial) and reproduce the endangered fish (as conservation).
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Judul: Karakteristik curah hujan DKI Jakarta dengan metode Empirical Orthogonal Function (EOF)
Abstrak: Curah hujan di Indonesia dipengaruhi oleh monsun yang digerakan oleh adanya sel tekanan tinggi dan sel tekanan rendah di benua Asia dan Australia secara bergantian. Dalam bulan Desember hingga Februari (DJF) dibelahan bumi utara terjadi musim dingin akibatnya terdapat sel tekanan tinggi di Benua Asia, sedangkan dibelahan bumi selatan pada waktu ini terjadi musim panas, akibatnya sel tekanan rendah di benua Australia. Karena ada perbedaan tekanan udara di kedua benua tersebut maka pada periode DJF bertiup angin dari tekanan tinggi di Asia menuju ke tekanan rendah di Australia, angin ini disebut monsun barat (winter monsoon). Dalam bulan Juni hingga Agustus (JJA) terdapat sel tekanan rendah di Asia dan sel tekanan tinggi di Australia yang menggerakan monsun timur (suntnter monsoon).
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