Judul: Optimasi Rendemen Serisin Kokon Ulat Sutera Samia cynthia ricini menggunakan Response Surface Methodology (RSM).
Abstrak: Kokon ulat sutera mengandung 2 protein utama yaitu fibroin dan serisin. Protein fibroin merupakan protein serat, sementara protein serisin merupakan perekatnya. Rendemen crude protein serisin pada ulat sutera Samia cynthia ricini dipengaruhi oleh bahan pelarut, suhu, dan waktu degumming. Oleh karena itu, diperlukan suatu formulasi yang tepat untuk menghasilkan rendemen serisin yang optimal. Metode yang biasa digunakan untuk tujuan optimasi adalah Response Surface Methodology (RSM). RSM dapat menganalisis permasalahan tentang respon yang dipengaruhi oleh beberapa variabel bebas. Daerah optimum (respon ordo pertama) ditentukan berdasarkan penelitian sebelumnya. Penelitian ordo kedua dirancang menggunakan Central Composite Design (CCD) dengan titik pusat pada suhu 95 °C dan waktu 40 menit pada program R. Degumming menggunakan bahan pelarut NaOH 0.033 N. Data rendemen serisin dianalisis menggunakan metode RSM pada program R versi 3.12 dengan packages 2.10. Optimasi yang dihasilkan adalah pelana kuda (saddle point). Optimasi rendemen serisin menghasilkan kondisi optimum pada suhu 113.2 °C dan waktu 33.77 menit yaitu sebesar 21.94±2.08%.
Keyword: degumming, rendemen, response surface methodology, Samia cynthia ricini, serisin

Judul: Characteristic Sericin Protein Cocoon of Eri  (Samia cynthia ricini) using Different NaOH Solvent Concentrations
Abstrak: Serisin merupakan protein globular penyusun kokon ulat sutra yang memiliki beragam manfaat di bidang pangan, kosmetik, farmasi, dan biomedis. Pada penelitian sebelumnya, rendemen serisin yang diperoleh menggunakan larutan degumming NaOH 0,1 N belum optimal. Penelitian ini bertujuan menganalisis rendemen protein, bobot molekul, dan susunan asam amino protein serisin kokon ulat sutra Eri dengan larutan degumming NaOH 0,25 N. Hasil ekstraksi protein serisin dengan larutan degumming NaOH 0,25 N menghasilkan rendemen serisin sebesar 23,6±4,1%. Hasil tersebut lebih baik dibandingkan bila menggunakan larutan degumming NaOH 0,1 N (4,8±1,5%). Bobot molekul protein serisin hasil isolasi memiliki rentang bobot molekul protein 15,68-45,70 kDa yang sesuai dengan kisaran bobot molekul serisin dari literatur. Hasil analisis HPLC menunjukan bahwa komponen asam amino utama protein serisin adalah alanina, glisina, dan tirosina., Sericin is a globular protein forming silkworm coccon that have various function in foods, cosmetics, pharmaceuticals, and biomedical industry. Previous research have used 0.1 N NaOH degumming solvent and considered not optimal in producing yields. This research purpose to analyze the protein yield, molecular weight, and amino acid composition of the Eri silkworm cocoon sericin protein using 0.25 N NaOH degumming solvent. The results of protein sericin extraction with 0,25 N NaOH degumming solvent resulted sericin yield of 23.6%±4,1. These result is better than using NaOH 0.1 N degumming solvent (4,80%±1,51). The molecular weight of isolation sericin protein is 15.68-45.70 kDa which corresponds to the range of molecular weights from the literature. The result of the HPLC analysis showed that the main amino acid components of the sericin protein is alanine, glisine, and tyrosine.
Keyword: Eri Silkworm Cocoon, NaOH, Solvent, Samia cynthia ricini, Sericin

Judul: Lejeuneaceae Subfamily Ptychanthoideae (Hepaticae) in  West Java
Abstrak: Subfamily Ptychanthoideae of the family Lejeuneaceae (Hepaticae) in West Java is still poorly known. Publications on this subfamily are out of dated and written in the 19th or early of 20th century. The last taxonomic treatment of Ptychanthoideae of West Java was in 1933, many species names have changed since then. Studies on Ptychanthoideae of West Java are urgently needed. Studies on the Ptychanthoideae were based on the specimens collected from West Java. A total number of 361 of specimens deposited in the Herbarium Bogoriense (BO), Herbarium Biotrop (BIOT), Gottingen Herbarium (GOET) and Bangi Herbarium (UKMB) were examined, those are old and several recent specimens collected in West Java. Recent collections were made from Telaga Warna, Ciater, Gunung Halimun Salak National Park, Cibodas Botanical Garden, Bodogol Education Center and Conservation (Gunung Gede Pangrango National Park), University of Indonesia Campus Depok, including several collections from Banten Province. Twenty four species of Lejeuneaceae subfamily Ptychanthoideae are known from West Java, belonging to 8 genera namely Acrolejeunea (Spruce) Schiffn. (2 species), Archilejeunea (Spruce) Schiffn. (1 species), Lopholejeunea (Spruce) Schiffn. (10 species), Mastigolejeunea (Spruce) Schiffn. (3 species), Ptychanthus Nees (1 species), Schiffneriolejeunea Verd. (1 species), Spruceanthus Verd. (2 species) and Thysananthus Lindenb. (4 species). Two new records of Ptychanthoideae: Mastigolejeunea indica Steph. and Thysananthus minor Verdoorn are reported for Java. A determination key to the species is presented including their descriptions and illustrations.
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