Judul: Characteristic of Nucleotide Sequence of COI Gene of Blue Swimming Crab Portunus pelagicus (Linnaeus, 1758) for Fisheries Management in WPP-NRI 711
Abstrak: Sumberdaya perikanan Indonesia yang bersifat multispesies dapat menimbulkan fenomena cryptic dan complex species, sehingga dibutuhkan metode yang akurat dalam pemastian spesies rajungan, yaitu dengan DNA barcoding. Penelitian ini bertujuan menentukan kepastian spesies dan menganalisis kondisi populasi rajungan (Portunus pelagicus) di perairan WPP-NRI 711 berdasarkan gen COI. DNA contoh rajungan berjumlah 10 individu yang didapatkan dari tiga lokasi, yaitu perairan Bintan, Bangka, dan Ketapang telah diisolasi. Kualitas untaian DNA yang baik digandakan dan diurutkan pada proses sekuensing. Seluruh contoh rajungan tervalidasi secara genetik sebagai spesies Portunus pelagicus. Komposisi basa nukleotida dari contoh rajungan didominasi oleh pasangan basa adenin-timin. Keragaman genetik rajungan di perairan Ketapang dan Bintan tergolong tinggi, sedangkan di perairan Bangka tergolong sedang. Ketiga lokasi penelitian memiliki nilai Fst yang tergolong rendah. Hasil penelitian menunjukkan bahwa populasi rajungan berada pada kondisi yang baik., Indonesia's multi-species fisheries resources can cause cryptic and complex species phenomena, so an accurate method is needed to determine the blue swimming crab species, such as DNA barcoding. This study aimed to determine the species certainty and to analyze the population status of blue swimming crab (Portunus pelagicus) based on the COI gene in WPP-NRI 711. A total of 10 blue swimming crab samples DNA obtained from three locations such as Bintan, Bangka, and Ketapang waters was isolated. High quality DNA strands are duplicated and sequenced. All of the samples have been genetically validated as Portunus pelagicus species. The nucleotide base composition of the COI gene from samples was dominated by adenine-thymine pairs. The genetic diversity value in Ketapang and Bintan are high, while in Bangka is moderate. All sampling sites have a low fixation index value. It can be concluded that the blue swimming crab populations are in a good condition.
Keyword: blue swimming crab, COI gene, molecular analysis

Judul: Keragaman genetik rajungan Portunus pelagicus (Linnaeus, 1758) di WPP NRI 572 dan 573 berdasarkan marka gen COI.
Abstrak: Rajungan merupakan anggota Crustacea yang memiliki nilai ekonomi dan peminat yang tinggi pada pasar lokal maupun ekspor. Hal tersebut menjadikan rajungan banyak ditangkap di perairan Indonesia. Tingginya Intensitas penangkapan rajungan tanpa adanya pengelolaan dapat mengakibatkan menurunnya keragaman genetik. Penelitian ini bertujuan untuk menganalisis kekerabatan dan keragam genetik rajungan yang tertangkap di Wilayah Pengelolaan Perikanan Negara Republik Indonesia (WPP-NRI) 572 (Labuan) dan WPP-NRI 573 (Cilacap-Banyuwangi) menggunakan marka gen COI sebagai dasar pengelolaan sumberdaya rajungan. Sebanyak 90 contoh rajungan dari lokasi penelitian di ambil 30 individu untuk di analisis molekuler. Seluruh contoh rajungan yang dianalisis memiliki nilai persen identifikasi sebesar 99-100% sebagai Portunus pelagicus. Nilai jarak genetik antarsampel menunjukan kekerabatan yang dekat karena memiliki nilai dibawah 3%. Pohon filogeni menunjukan hasil kekerabatan rajungan dari tiap lokasi sangat dekat serta memiliki keragaman genetik yang tergolong sedang dan tinggi. Upaya pengelolaan yang dapat dilakukan yaitu mempertahankan tingkat eksploitasi dan evaluasi dengan meningkatkan monitoring pada WPP-NRI 573, sedangkan pada WPP-NRI 572 dilakukan penurunan intensitas penangkapan., Blue swimming crab (BSC) is a member of Crustacea which has high economic value and interest in local and export markets. Because of those reasons, blue swimming crabs are caught in Indonesian waters. The high intensity of crab fishing without management can decreased the genetic diversity. This study aims to analyze the kinship and genetic diversity of blue swimming crabs in the Fisheries Management Areas of the Republic of Indonesia ( FMA-RI) 572 (Labuan) and FMA-RI 573 (Cilacap-Banyuwangi) using COI gene markers as the basis for blue swimming crab resource management. A total of 30 individuals out of 90 samples taken were used for molecular analysis. All samples of BSC analyzed had a percent identification value of 99-100% as Portunus pelagicus. The value of the genetic distance between samples shows close kinship because it has a value below 3%. The phylogenetic tree shows the results of blue crab kinship from each location which are very close and have moderate and high genetic diversity. Management efforts can be carried out to maintain the level of exploitation and evaluation by increasing monitoring at FMA-RI 573, while at FMA-RI 572 a reduction in fishing intensity is carried out.
Keyword: Blue swimming crab, COI, genetic distance, genetic diversity

Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4