Judul: Manajemen Sumber Daya Keluarga dan Ketahanan Keluarga Lanjut Usia
Abstrak: This study is conducted to analyze the influence of family resource management (FRM) to family strength on aging families. The data was collected on June-July 2012 purposively at three villages (Ciherang, Cikarawang, and Babakan) which are included to five areas with the largest aging family populations in Bogor. Interviews were conducted on 34 early-elders (60-75 years old) who still have spouses and have no dependents. FRM was measured with human resource management, time management, and financial management of all phases of the family. Family strength was measured with physical strength, psychological strength, and social strength. The result showed that average of family strength aging families had performance by three-quarters of family strength ability (75% for physical strength, 68% for psychological strength, dan 83% for social strength). Performance index of FRM ability had only achieve half of FRM indicators (81% for human resource management, 19% for time management, and 71% for financial management). FRM had influence on family strength. Length of education and of marriage and percapita income had a significant positive influence on family strength.
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Judul: Resource Management and Family Strength of the Participants of Community Empowerment Program in Rural Areas in Bogor District
Abstrak: Program pemberdayaan merupakan salah satu bentuk intervensi yang dilakukan oleh berbagai pihak untuk menanggulangi permasalahan yang dihadapi keluarga. Prioritas kegiatan pemberdayaan keluarga dan target pencapaian utamanya adalah ketahanan keluarga. Untuk mencapai target tersebut, maka salah satu upaya penting dalam strategi pemberdayaan adalah proses pendidikan, baik yang bersifat formal maupun nonformal. Dalam pelaksanaannya program pemberdayaan tidak cukup hanya memberi bantuan materi saja karena hal tersebut tidak dapat menyelesaikan permasalahan secara tuntas dan sifatnya sesaat. Agar keluarga dapat bertahan dalam menjalankan kegiatannya sehari-hari sehingga tercipta ketahanan keluarga di masa krisis maka sumberdaya keluarga yang terbatas maupun sumberdaya tambahan dari program pemberdayaan, baik yang bersifat materi maupun nonmateri harus dikelola dengan baik melalui manajemen sumberdaya keluarga. Perbedaan kemampuan keluarga dalam mengelola sumberdaya, tentunya akan menghasilkan variasi keluarga dalam menjalani hidup di masa krisis, yang selanjutnya melahirkan variasi ketahanan keluarga pada setiap keluarga. Penelitian ini bertujuan untuk : (1) Menganalisis praktik manajemen sumberdaya keluarga peserta program pemberdayaan masyarakat di pedesaan, (2) menganalisis faktor-f~or yang mempengaruhi manajemen sumberdaya keluarga peserta program pemberdayaan masyarakat di pedesaan, (3)Menganalisis faktor yang mempengaruhi ketahanan keluarga perserta program pemberdayaan masyarakat di pedesaan, dan (4) Merumuskan model dan strategi untulc peningkatan manajemen swnberdaya dan ketahanan keluarga anggota peserta program pemberdayaan masyarakat di pedesaan., In order to achieve family strength among family members, the family needs to implement family resource management either material or non material efficiently. This research was aimed to (1) analyze the family resource management practices, (2) analyze the factors influenced family resource management, (3) analyze the factors influenced family strength, and (4) formulate model and strategy to improve family resource management and family strength. The research was design as a crossectional study using survey method to collect data from 300 respondents. The research sites were in Dramaga, Leuwisadeng, and Pamijahan subdistrict in Bogor District from February to October 2010. The analysis used descriptive statistic, and inferensial statistic by using Structural Equation Modeling (SEM) helped by software LISREL 8.70. The results showed that the highest percentage (40,3%) of the family resource management quality categorized as moderate level and 35,3 percent of the family categorized as low level. It was found that social characteristics of the family, gender relation and family participation toward extension practices directly influenced family resource management. In general the highest percentage (54,7%) of family strength categorized as moderate level and found 28,0 percent of the family categorized as low level. It was found that quality of gender relation, family resource management, and family demographic characteristics were directly and positive influenced to family strength. Whereas, the number of family members was directly negative influenced to family strength: Recommendation to increased family strength could be reached by improving gender relations, productivity of spouse, family financial management and time allocation pattern
Keyword: family resource management, family strength, community empowerment, extension

Judul: Characterization and Expression of Tomato infectious chlorosis virus Coat Protein Gene in Escherichia coli
Abstrak: Tomato infectious chlorosis virus (TICV) is a member of Crinivirus genus. TICV infects tomato crop in Garut and Cipanas, West Java Indonesia. Tomato initially showed bright interveinal yellowing symptoms. The coat protein gene (CP-TICV) was amplified by RT-PCR from total RNA extracted from infected tomato leaves and the amplified fragment was cloned and completely sequenced. The fragment was subsequently subcloned into the pET-21b expression vector. The recombinant plasmid was transformed to Escherichia coli strain BL21(DE3)pLysS to express the coat protein. The coat protein fused to a 6xhistag, was purified by affinity chromatography using a NiNTA spin column. The identity of the purified protein was confirmed by SDS-PAGE. In this experiment, 792 bp of CP-TICV gene of TICV virus has been successfully cloned, sequenced, and expressed in E. coli. Based on nucleotide sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain strain and 99% identity to North America, France, and California strains and based on amino acid sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain, North America, France strains and 99.2% identity to California strain. CP-TICV showed over expressed in E. coli when it is induced with 1 mM IPTG and incubated at 37oC. Purified CP-TICV-Histag recombinants protein sized 29 kDa based on SDSPAGE analysis.
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