Judul: Kolonisasi dan proliferasi sel testikular ikan nila putih yang ditransplantasikan ke ikan nila hitam triploid
Abstrak: The technology of fish testicular cells transplantation had been established to create a surrogate broodstock. This technology can also be utilized to generate YY-progenies that are useful to produce all-male fish. In this study, as a first step towards all-male tilapia production, white Nile tilapia testicular cells was transplanted into peritoneal cavity of triploid black tilapia larvae. A specific primer was designed to identify colonization and proliferation of transplanted cells in recipient fish using PCR method. Analysis of gonadosomatic index (GSI) was also performed to determine the transplanted cells proliferation in recipient gonad. The result of PCR analysis showed a specific DNA amplification product in the gonad of transplanted fish. The specific DNA donor was detected on the recipient gonad 90 dpt (days post transplantation) and it was not detected on 30 and 60 dpt. The GSI analysis showed that GSI recipient was increased and relatively similar with diploid non-transplanted Nile tilapia and it was higher than triploid non-transplanted Nile tilapia. These results showed that the donor cell could be colonized and proliferated in triploid black tilapia gonad. Thus, it is likely to obtain a broodstock that is able to produce donor-derived gamete in the near future.
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Judul: Xenotransplantation of giant gourami testicular germ cells into larvae of nile tilapia
Abstrak: We were attempting to develop testicular germ cell (TGC) transplantation as a tool to produce surrogate broodstock of commercially valuable fish and long generation time species. Giant gourami testis had been used as a model for donor and Nile tilapia larvae as recipient. The research was conducted to study the competency of giant gourami TGC as donor and Nile tilapia larvae as recipient for xenotransplantation of giant gouramy TGC into larvae of Nile tilapia. We developed TGC xenotransplantation system by some steps as follow : 1) The characterization of spermatogonia to identify optimal donor of giant gourami based on body weight using histological approach, 2) The determination of dissociation method for giant gourami testicular tissue by comparing two different composition of dissociation medium with 5 hours incubation time, 3) Optimizing the timing of intraperitoneal TGC transplantation into peritoneal cavity of 1, 3, 5 and 7 days post hatching (dph) recipient by investigating the colonization efficiency of donor cell labelled PKH 26 fluorescent membrane dye under fluorescent microscope and of molecular technique using growth hormone of giant gourami specific primer, 4 ) Analyzing the proliferation of spermatogonia colonized in recipient gonad using molecular approach, and 5) The evaluation of TGC isolated from testis of giant gourami which preserved at 4 oC using NaCl 0.7% for 6, 12, 24 and 48 hours and then transplanted into peritoneal cavity of recipient. The result showed that the donor with abundant spermatogonia stem cell and A type spermatogonia (cell diameter = 14.43–20.53 μm) were found in giant gourami with body weight ranged from 500–1000 g. The dissociation method produced high number of spermatogonia with high viability was one used medium PBS containing trypsin, DNase, CaCl2, HEPES, FBS and incubated for 3 hours. The highest colonization efficiency was observed at 3 dph recipient (61.1±34.71%) suggesting that 3 dph Nile tilapia larvae was the optimum recipient for transplantation. Intraperitoneally transplanted xenogenic spermatogonia efficiently colonized the ovary as well with sex ratio male out of female was 1:1, and possibly proliferated indicated by cell cluster forming and the increase of DNA concentration of donor in recipient testis during time interval 1 month to 2 or 3 month pt. The successful colonization of spermatogonia isolated from preserved testis were also observed with colonization efficiency not differed significantly as from non preserved testis. In conclusion : the testis of giant gouramy was composed of cells that had competency as donor for xenotransplantation using Nile tilapia larvae as recipient.
Keyword: Sel Testikular, Osphronemus goramy, Ikan Gurami, ikan nila, kolonisasi, proliferasi, preservasi

Judul: Peran Masyarakat dalam Pengendalian Kebakaran Hutan di KPH Cepu Jawa Tengah.
Abstrak: Kebakaran Hutan merupakan salah satu bentuk kerusakan hutan. Penyebab terjadinya kebakaran hutan biasanya karena adanya pembukaan areal hutan yang digunakan untuk lahan pertanian. Faktor terjadinya kebakaran hutan ada 2 yaitu, faktor alami dan manusia. Faktor alami disebabkan adanya letusan gunung api dan petir, kemudian faktor manusia dikarenakan adanya pembukaan kawasan hutan untuk areal pertanian. Penelitian ini bertujuan untuk mengkaji upaya dan peran mayarakat dalam pengendalian kebakaran hutan di KPH Cepu Jawa Tengah. Kejadian kebakaran hutan tertinggi terjadi pada tahun 2015 dengan frekuensi 40 kali dengan luas total lahan yang terbakar 140.0 Ha. Kebakaran hutan pada tahun 2015 merupakan kejadian kebakaran hutan terbesar di KPH Cepu yang merugikan sebesar Rp 38 292 909. Bentuk pengendalian yang dilakukan oleh pihak KPH Cepu sendiri yaitu dengan adanya penyuluhan atau sosialisasi terkait kebakaran dan bagaimana cara penanggulangan apabila terjadi kebakaran. Bentuk penyuluhan secara langsung diberikan kepada masyarakat perkumpulan LMDH, perkumpulan RT, dan perkumpulan lainnya yang tidak ditentukan waktunya. Partisipasi masyarakat sangat tinggi dalam kegiatan pencegahan maupun pemadaman di KPH Cepu Jawa Tengah.
Keyword: Faktor, partisipasi, pengendalian kebakaran hutan