Judul: Production Management of Banana Seedlings (Musa spp.) at Tissue Culture Laboratory of Lebak Bulus DKI Jakarta
Abstrak: Kultur jaringan merupakan metode terbaik untuk memproduksi bibit pisang yang seragam dan bebas penyakit. Kebun Bibit dan Laboratorium Kultur Jaringan Lebak Bulus merupakan instansi yang melakukan produksi bibit pisang berbagai kultivar. Kegiatan magang memiliki tujuan umum untuk mempelajari manajemen laboratorium kultur jaringan pisang dan tujuan khusus untuk mempelajari regenerasi eksplan pisang di media tanam yang ditambahkan zat pengatur tumbuh (ZPT) pada setiap tahapan kultur jaringan yang berbeda. Tujuan umum dicapai dengan melakukan aspek manajerial sementara tujuan khusus dicapai melalui pengamatan aspek khusus. Hasil pengumpulan data pada aspek manajerial menunjukkan bahwa: (1) metode kultur jaringan yang dikembangkan di Laboratorium Lebak Bulus sudah sangat baik, (2) laboratorium perlu dirancang dengan tata ruang yang efektif, (3) organisasi dan koordinasi yang jelas dari atas ke bawah dan dari laboratorium ke kebun bibit sangat diperlukan, (4) manajemen limbah kimia sangat penting dan harus diperhatikan. Hasil pengamatan pada aspek khusus menunjukkan bahwa: (1) komposisi media tanam berbeda sesuai tahapan kultur, (2) tingkat induksi tunas saat tahap multiplikasi sangat mempengaruhi jumlah total produksi bibit, (3) benzyl amino purin (BAP) 3 mg l-1 sangat baik dalam meningkatkan induksi tunas pisang pada media MS, (4) thidiazuron (TDZ) 0,01 mg l-1 memiliki pengaruh yang sama terhadap tunas dengan auksin pada media MS, yaitu sangat baik dalam pembesaran tunas dan pembentukan organ, (5) genom dan ekspresi genetik kultivar mempengaruhi pertumbuhan tunas, namun pengaruhnya tidak sebesar pengaruh hormon tumbuhan., Tissue culture is the best method for producing uniform and disease-free banana seedlings. Lebak Bulus Nursery and Tissue Culture Laboratory is an institution that produces banana seedlings of various cultivars. The internship has a general objective of studying management of the banana tissue culture laboratory and a specific objective to study the explant regeneration of bananas in the growing media added with plant growth regulators (PGR) at each different stage of tissue culture. General objectives are achieved by carrying out managerial aspects, while specific objectives are achieved through observing specific aspects. The results of data collection on managerial aspects indicate that: (1) the tissue culture method developed at the Lebak Bulus Laboratory is proper, (2) the laboratory needs to be designed with an effective spatial plan, (3) clear organization and coordination from top to bottom and from the laboratory to the nursery is very necessary, (4) chemical waste management is very important and must be considered. The results of observations on specific aspects showed that: (1) the composition of the growing media was different according to the stage of culture, (2) the rate of shoot induction during the multiplication stage greatly affected the total amount of seedling production, (3) benzyl amino purine (BAP) 3 mg l-1 is good in increasing banana shoot induction on MS media, (4) thidiazuron (TDZ) 0.01 mg l-1 had the same effect on shoots as auxin on MS media, which was very good in bud enlargement and organ formation, (5) genome and genetics expression of cultivar affect shoot growth, but the effect is not as prominent as the influence of plant hormones.
Keyword: BAP, genome, multiplication, shoot induction, TDZ

Judul: Pengaruh sitokinin (TDZ) dan auksin (IAA dan NAA) terhadap multiplikasi nenas, Ananas comosus L. Merr. cv. Queen dalam perbanyakan kultur jaringan
Abstrak: The objective of this research was to study the effect of Cytokinin (TDZ) and Auxin (IAA and NAA) as growth regulators in shooting multiplication capacity of shoot base pineapple explant. This research was conducted in tissue culture laboratory at Tropical Fruits Study Center. IPB. Baranangsiang. Bogor. since January until September 2004. Planting material of 5-10 mm base shoot of third subculture Queen pincapple's planlet were cultured in shooting induction medium consisted of MS suplemented with Auxin (IAA and NAA) and TDZ in various concentrations. The experiment was set up in factorial design with two factors, Auxin (5 levels: 0 mg/1. 0.1 mg/l IAA, 0.01 mg/l IAA, 0.1 mg/I NAA and 0.01 mg/l NAA) and TDZ (6 levels: 1x10 mg/l. 1x10 mg/l, 1x10 mg/l, 1x10 mg/l, 1x10 mg/l and 1x10 mg/l). There were 30 combinations treatment. Each treatment was consist of 4 replicates, containing 6 explants per replicate, so that found 720 unit. After 20 weeks in shooting induction medium, explants were transferred to MSO medium for shooting multiplication. Each treatment was consist of 12 replicates. In shooting induction phase, the percentage and the the number of shoot. root. nodule and chap nodule were examined. After subculture to MS free medium, the number of axillar shoot, adventitious shoot and chap nodule were examined every weeks. The weight of nodule were examined in the first and last week of observation. Meanwhile. the number of shoot per gram nodule weight were also examined in the last week of observation. The data were statistically analyzed using completely randomized design and the means were compared with Duncan's Multiple Range Test (DMRT) procedure at 5% level of significance. In the first week of the culture shoot induction medium. explants have shown its growing which marked with crosscut leaves elongation and new leaves formation. In almost treatments, shoot started to be formed at 2 weeks after culture (MST) and rooted at 3 MST. except for the treatments of TDZ at concentration 1x10 mg/l. which is combined with various Auxin concentrations. Explants grown in media supplemented with 1x10 mg/l. 1x105 mg/l, 1x10 mg/l. 1x10 mg/l TDZ in different concentrations of Auxin. initially responded by shoot and root formation with no nodules. There were little number of shoots (with amount of mean less than two) that formed in these culture medium by the end of perception week in both phases, shoot induction phase and shoot multiplication phase. Although the shoots cultured in media supplemented with low concentrations of TDZ (below 1x102 mg/l) were fewer in number. but the shoots were longer and have better quality than those formed in media supplemented with high level concentrations of TDZ (above 1x10 mg/) in the same perception week. Shoots became capable of rooting at combinations of 1x10- 1x10 mg/l TDZ with different concentrations of Auxin, but 1x10 mg/l without Auxin gave rise to the best rooting...dst
Keyword: 

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
Keyword: