Judul: DNA Barcoding dan Evolusi Taxa Pari Gergaji (Anoxypristis cuspidata) dan Pari Kekeh (Rhynchobatus laevis) di Perairan Merauke, Papua
Abstrak: Indonesia dikenal dengan negara yang memiliki keanekaragaman Elasmobranchii yang tinggi. Pari gergaji di dunia terdapat lima spesies, empat ada di Indonesia sedangkan Pari kekeh memiliki 10 spesies, lima ada di Indonesia. Tujuan penelitian adalah untuk mengidentifikasi spesies dan evolusi taxa pari gergaji dan pari kekeh menggunakan DNA barcoding di perairan Merauke, Papua. Primer yang digunakan pada penelitian ini adalah adalah primer universal Fish F1 untuk forward dan Fish R1 untuk reverse. Identifikasi tingkat spesies dilakukan menggunakan BLAST pada genbank di NCBI. Hubungan sejarah evolusi taxa menggunakan metode Neighbor-joining dan Maximum likelihood. Identifikasi celah menggunakan metode ABGD untuk mendapatkan partisi dan jumlah spesies kandidat. Dari total 15 sampel pari yang ditemukan, 12 spesies merupakan Rhynchobatus laevis dan 3 spesies Anoxypristis cuspidata. Pohon filogenetik merekontruksi dua clade yang terbentuk yang terdiri dari clade pari gergaji dan pari kekeh. Pemisahan cabang menjadi dua kelompok spesies dari satu garis yang sama mempresentasikan sebuah pemisahan garis evolusi ke dalam dua spesies yang berbeda., Indonesia is known as a country that has high Elasmobranchii diversity. There are five Sawfish species in the world, four are found in Indonesia, and while there are 10 wedgefish species, five are found in Indonesia. The research objective was to identify the species and evolution taxa of pointed sawfishes and wedgefishes by using barcoding DNA in Merauke waters, Papua. Primers used in this study are Fish F1 universal primer for forward and Fish R1 universal primer for reverse. Species level identification was carried out using the BLAST on the genbank at NCBI. Historic relationship between taxa evolution uses the neighbor-joining and Maximum likelihood. Gap identification uses the ABGD method to obtain the partition and number of candidate species. Of the total 15 samples of rays found in Merauke waters, 12 species were Rhynchobatus laevis and 3 species Anoxypristis cuspidata. The phylogenetic tree reconstructs two clades that are formed, consisting of clades of sawfish and wedgefish. The splitting of branches into two groups of species from the same line represents a splitting of the evolutionary line into two distinct species.
Keyword: DNA barcoding, Elasmobranchii, evolusi taxa

Judul: DNA Barcoding dan Rekonstruksi Filogenetik Spesies Hiu yang Didaratkan di Pelabuhan Perikanan Pantai Muncar, Banyuwangi, Jawa Timur
Abstrak: Sharks are one of a fisheries commodity that are exploited on a large scale because of their very high economic value. The identification of sharks is very problematic, because specimens has similarity in morphology and mostly have had key diagnostic features removed. This study aimed to identify and to review the status of sharks landed in Muncar Fisheries Landing Site and see the trend of catches in 2012 to 2013, and also to reconstruct the shark species that were landed at South Java fishing port using molecular approaches. The DNA amplification was using Cytochrome Oxidase I locus mitochondrial. A total of 7 species was identified including Alopias pelagicus, Carcharhinus falciformis, C. sorrah, C. amblyrhynchos, Galeocardo Cuvier, Atelomycterus marmoratus, and Spyrna lewini. The diversity of shark species that landed in Muncar during the last 2 years has been decreased. The identified sharks species in this study sites were about 18% of all Indonesian sharks. The result of this study is expected help the Government to manage shark fisheries in Indonesia
Keyword: Muncar, Conservation, Phylogenetic, DNA barcoding, Sharks

Judul: Natrium lignosulfonat sebagai agen pendispersi pada mortar
Abstrak: Lignosulphonate, a modification product of lignin waste from pulp and paper industry can be used as water reducer in mortar mixture. This substance can influence the mechanical characteristic of mortar such as diameter of flow, flexural strength, compressive strength, and setting time of cement paste. Lignosulphonate shows it function as dispersant on cement hydration at 3 days that analyzed with x-ray difractometer. Research result show that flow of fresh mortar rise with increase concentration (from weight of cement) of synthetic sodium lignosulphonate (NaLS). It show that mortar homogenity also increasing. The result of flexural strength and compressive strength test use synthetic NaLS 0.1-0.3% from weight of cement show that addition synthetic NaLS 0.2% is the best result because it has the biggest of flexural strenght and compressive strength value. Initial setting time of cement paste increase, about 50% with addition of synthetic NaLS 0.2%. Whereas the final setting time decrease, about 8%. Cement hydration test show increase hydration product peak, Ca(OH)2 dan calcium silicate hydrate (C-S-H).
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