Judul: Pengembangan Spektrofotometer Sinar Tampak (KuantiVis) Berbasis Komponen Elektronik Umum dan Uji Kinerjanya
Abstrak: Spectrophotometer can be developed by replacing the main components with electronic components that are easy to find. This study aims to develop visible spectrophotometer (KuantiVis) device using light emitting diode lamp as light source, optical layer of digital versatile disks as grating, and web camera with charge-couple device sensor as detector. KuantiVis was tested for its performance based on linearity, limit of detection, limit of quantitation, and accuracy parameters using a standard of chromium. The optimum result showed that KuantiVis was made with dimensions of 33 × 16 × 5 cm and distance between web camera and source of light was 25 cm. The result showed that linearity of data based on R2 value was 0.9973, limit of detection was 0.1231 ppm, limit of quantitation was 0.4102 ppm, and recovery was 103.19%. The performance was not much different from the performance of Spectronic 20D+ and Genesys spectrophotometer. The KuantiVis was applied for determining borax concentration in wet noodles sample. Concentration of borax in sample was 28.60 μg/gram samples. The result of F test and t test showed that the data was not significantly different with that of Spectronic 20D+ and Genesys spectrophotometer.
Keyword: Bogor Agricultural University (IPB), visible spectrophotometer, light emitting diode, digital versatile disks, charge-couple device, borax

Judul: Development of Portable Spectrophotometers and Performance Evaluation of Ponceau 4R Food Colorants Substances
Abstrak: Spektrofotometer dapat dikembangkan dari komponen elektronik yang tersedia secara umum menjadi spektrofotometer portabel. Penelitian ini bertujuan mengembangkan spektrofotometer jinjing dan menentukan uji kinerja analitiknya. Spetrofotometer jinjing yang disusun oleh komponen utama yang terdiri dari lampu light emitting diode sebagai sumber cahaya, lapisan optik digital versatile disks sebagai kisi, web camera Logitech c920 sebagai detektor dan akuisisi data melalui perangkat lunak Thereminospectro. Uji kinerja spektro menggunakan larutan standar Ponceau 4R dan dibandingkan dengan spektrofotometer komersial Hitachi Spectro U-2800. Spektrofotometer jinjing dengan ukuran optimum menghasilkan spektrum larutan standar yang sebanding dari hitachi spectro u-2800. Keduanya menunjukkan linearitas yang sangat baik karena memenuhi syarat keberterimaan linearitas pada metode spektrofotometri. Dengan demikian, spektrofotometer jinjing yang dikembangkan dapat menjadi alternatif untuk spektrofotometer komersial. Kata kunci: digital versatile disks, light emitting diode, ponceau 4R, spektrofotometer, web camera, Spectrophotometers can be expanded from commonly available electronic components to portable spectrophotometers. This study aims to develop a portable spectrophotometer and determine its analytical performance test. A portable spectrophotometer composed of main components consisting of a light-emitting diode lamp as a light source, an optical layer of digital versatile disks as a grid, a Logitech c920 web camera as a detector, and data acquisition via the Thereminospectro software. Spectro performance test using standard solution Ponceau 4R and compared with a commercial spectrophotometer Hitachi Spectro U-2800. The portable spectrophotometer with the optimum size produces a standard solution spectrum comparable to that of the Hitachi Spectro u-2800. Both of them show very good linearity because they meet the requirements for linearity acceptance in the spectrophotometric method. Thus, the developed portable spectrophotometer can be an alternative to commercial spectrophotometers. Keywords: digital versatile disks, light emitting diode, ponceau 4R, spectrophotometer, web camera
Keyword: Spektrofotometer UV-VIS, Spektrofotometer jinjing, LED, DVD, Webcamera, Ponceau 4R

Judul: Posisi Filogenetik Kura-Kura Carettochelys Insculpta  (Reptillia: Testudinata)
Abstrak: Carettochelys insculpta is an endemic freshwater turtle of southern Papua and northern Australia. Phylogenetic position of this turtle is remain unresolved until now. Most of the research conducted show close relationships of C. insculpta and members of Trionychidae family. However, some of the research show distance between C. insculpta and members of Trionychidae family. This research is conducted to analyze phylogenetic relationship between C. insculpta and other members of Testudinata using 12S rRNA, 16S rRNA, and both gene as markers. DNA samples were obtained from three different organisms of C. insculpta. Two species used as outgroups were Alligator mississippiensis and Iguana iguana. Phylogenetic analysis was carried out using Neighbor-Joining method with Felsenstein 1981 (F81) modeltest. A polytomy tree of Testudinata was produced by using 12S rRNA gene. However, analysis using 16S rRNA and both genes as markers confirmed that C. insculpta was located between the Chelonidae and the Trionychidae family.
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