Judul: Ekstraksi dan pemurnian protease dari Bacillus pumillus (Y1).
Abstrak: Bacillus adalah bakteri yang sangat potensial untuk dijadikan sumber enzim protease. Bacillus yang digunakan dalam penelitian ini adalah jenis B. pumillus (Y1) yang diisolasi dari limbah cair tahu (Likumahwa, 1993). Sedangkan penelitian ini bertujuan untuk melakukan ekstraksi dan pemurnian enzim protease dari B. pumillus (Y1) dengan menggunakan berbagai kolom kromatografi [DEAE (dietilaminoetil) Sephadex A-50 dan Amberlite] untuk mendapatkan enzim protease yang relatif murni. Dalam penelitian ini dilakukan fermentasi bakteri untuk menghasilkan enzim protease dalam limbah cair tahu dengan penambahan 2% skim, lalu diikuti ekstraksi enzim dengan garam ammonium sulfat 55% (w/v), besi (III) klorida, aluminium (III) klorida dan aseton dan dilarutkan kembali dalam buffer sodium sitrat, sodium ditionat dan fosfat 0.01 M pH 8.0, kemudian didialisis dalam kantung selofan. Dialisat lalu dikromatografi dalam resin penukar anion (Amberlite IRA 400 dan DEAE Sephadex A 50) dan penukar kation (Amberlite DP-1). Fraksi-fraksi yang diperoleh, dipekatkan dengan pengeringan beku dan selanjutnya dielektroforesis dalam gel SDS-poliakrilamid. ...
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Judul: Pemurnian dan karakterisasi protease dari Bacillus punilus
Abstrak: Penelitian ini bertujuan mempelajari sifat-sifat enzim protease ekstraselular dari Bacillus pumilus yang diisolasi dari limbah cair tahu di Indonesia. Galur murni yang digunakan Bacillus pumilus Y1 merupakan hasil isolasi dari Likumahua Yosi T. Maria (1993). Karakteristik enzim protease yang diamati meliputi pengaruh suhu, pH, jenis dan konsentrasi ion logam terhadap aktivitas enzim, param- eter kinetika reaksi ( Km dan Vmax ), dugaan berat molekul, dan tingkat kemurnian yang dapat dicapai setelah pemurnian melalui pengendapan garam, dialisis dan kromatografi. Produksi enzim dilakukan dengan proses fermentasi sistem curah ( batch). Limbah cair tahu sebanyak 800 ml (pH 7), ditambah 5% susu skim digunakan sebagai medium. untuk pertumbuhan Bacillus pumilus. selam 12 jam Fermentasi dilakukan dalam bejana fermentor pada suhu 37° C, dengan agitasi 100 rpm, dengan aerasi 1 VVM dan pH 7. Pemanenan enzim protease dilakukan dengan sentrifugasi pada 2000 rpm selama 20 menit pada suhu 0° C. Supernatan yang dihasilkan disebut ekstrak kasar…dst
Keyword: Protease, Bacillus punilus, Microbial enzym, Permentation

Judul: Characteristic of Overshooting Top (OT) Based on  Himawari  8  Satellite  as  a  Convective  Cloud  Feature  that  Generates  Extreme Weather in the Java Island Region
Abstrak: Overshooting Top (OT) is a feature at the top of a convective cloud that is associated with extreme weather. Research on OT in tropical Indonesia is still very limited. The existence of geostationary satellites with wide area coverage and fast frequency observations is useful for observing the fast dynamics of the OT life cycle. Therefore, this study intends to analyze the relationship between OT and extreme weather, updraft and thermodynamic conditions of OT when extreme weather occurs, the best objective method for detecting OT in the day time and night time, and the spatiotemporal distribution of OT in the tropical region of Java Island during the 2018-2021 period. Based on visual analysis using visible channel and Himawari 8 sandwich products, 87% of extreme weather events in Java were accompanied by the appearance of OT. It indicates that the presence of the OT pattern is a convection cloud pattern or feature that is closely related to extreme weather events for the Java Island region. The existence of a time lag between the appearance of OT and extreme weather events on Java Island indicates that the OT of convection cloud can be used as a precursor or nowcasting for the appearance of extreme weather. Most of the convective cloud OT features during extreme weather events on Java Island have altitude that were only able to exceed the altitude of the equilibrium level and few were able to exceed the tropopause level of 9 km – 16 km. The difference in atmospheric conditions during extreme weather events on Java Island, which is in the tropics and subtropics, is a strong and unstable convective energy condition characterized by a larger CAPE, as well as unstable KI, LI, SI and TTI values. It is offset by kinematic factors in the form of a weak vertical wind shear (bulk wind shear 0-6 km) and a higher freezing point height. The difference between extreme weather events in the Java Island region and other tropical regions compared to subtropical regions is the strong updraft speed of convection clouds. The IRW Texture COMB algorithm based on the IRW-Texture Gradient modification and the COMB algorithm shows a significant increase in quality for detecting OT and non-OT pixels in Java. Rainy days with rainfall > 50 mm/day are very common in areas with an altitude of > 500 m. On the other hand, the level of convection activity which is indicated by the presence of OT and lightning is significantly reduced in this area. OT of convective clouds appear more frequently during the DJF season and less frequently during the JJA season. OT often appears during the day time and rarely at night time. Most OTs appear in the central part, away from the south and north coasts of Java Island during the day, while OTs that appear near the south and north coasts of Java Island can appear in the morning or afternoon.
Keyword: convective clouds, extreme weather, Overshooting Tops, Himawari 8 Satellite