Judul: Kultur In Vitro Sel-sel Fibroblas Fetus Tikus
Abstrak: Research has been conducted on the in vitro culture of rat fetal fibroblast using Dulbeccos’ Modified Eagle Medium supplemented with 3mM sodium bicarbonate, 10% fetal calf serum and 50 μg/ml gentamycin (mDMEM) in CO2 incubator at 37oC. Evaluation was done on the proliferation rate and the protein production after several passages. The growth of the fibroblast was confirmed by their morphology. The proliferation rate was done based on the population doubling time (PDT) by counting the number of cells in each passage by using Improved Neubauer hemocytometer. Identification of proteins was done quatitatively using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Based on morphological observation the cell grown in in vitro culture were the fibrocyte and fibroblast and showed increasing of homogenicity after several passages. Based on the PDT, the proliferation rate was increased by each passages. Fibroblast also secrete proteins remain until passage 9 based on the results of electrophoresis. It can be concluded that the growth and protein secretion of rat fetal fibroblast in in vitro culture can be maintained up to 9 passages.
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Judul: Kultur in vitro sel-sel fibroblas fetus tikus
Abstrak: Penelitian yang dilakukan berupa kultur in vitro sel-sel fibroblas fetus tikus menggunakan Dulbeccos’ Modified Eagle Medium yang dimodifikasi dengan penambahan 3mM natrium bikarbonat, 10% fetal calf serum, dan 50 μg/ml gentamisin (mDMEM) di dalam inkubator CO2 pada suhu 37ºC. Evaluasi dilakukan terhadap tingkat proliferasi dan produksi protein setelah beberapa pasase. Pertumbuhan sel-sel fibroblas diidentifikasi melalui pengamatan morfologi. Tingkat proliferasi sel ditentukan berdasarkan population doubling time (PDT) dengan menghitung jumlah sel pada setiap pasase menggunakan hemositometer Improved Neubauer. Identifikasi protein dilakukan dengan metode Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Berdasarkan pengamatan morfologi, sel yang berkembang di dalam kultur in vitro adalah sel fibrosit dan sel fibroblas dan memperlihatkan peningkatan homogenitas setelah beberapa pasase. Berdasarkan nilai PDT, tingkat proliferasi sel juga meningkat seiring pasase. Sel fibroblas juga tetap mensekresikan protein hingga pasase 9 berdasarkan hasil elektroforesis. Dengan demikian dapat disimpulkan bahwa pertumbuhan dan sekresi protein dari kultur in vitro sel-sel fibroblas bisa dipertahankan sampai 9 pasase.
Keyword: Kultur sel, Fibroblas, Bogor Agricultural University

Judul: Characteristic of Overshooting Top (OT) Based on  Himawari  8  Satellite  as  a  Convective  Cloud  Feature  that  Generates  Extreme Weather in the Java Island Region
Abstrak: Overshooting Top (OT) is a feature at the top of a convective cloud that is associated with extreme weather. Research on OT in tropical Indonesia is still very limited. The existence of geostationary satellites with wide area coverage and fast frequency observations is useful for observing the fast dynamics of the OT life cycle. Therefore, this study intends to analyze the relationship between OT and extreme weather, updraft and thermodynamic conditions of OT when extreme weather occurs, the best objective method for detecting OT in the day time and night time, and the spatiotemporal distribution of OT in the tropical region of Java Island during the 2018-2021 period. Based on visual analysis using visible channel and Himawari 8 sandwich products, 87% of extreme weather events in Java were accompanied by the appearance of OT. It indicates that the presence of the OT pattern is a convection cloud pattern or feature that is closely related to extreme weather events for the Java Island region. The existence of a time lag between the appearance of OT and extreme weather events on Java Island indicates that the OT of convection cloud can be used as a precursor or nowcasting for the appearance of extreme weather. Most of the convective cloud OT features during extreme weather events on Java Island have altitude that were only able to exceed the altitude of the equilibrium level and few were able to exceed the tropopause level of 9 km – 16 km. The difference in atmospheric conditions during extreme weather events on Java Island, which is in the tropics and subtropics, is a strong and unstable convective energy condition characterized by a larger CAPE, as well as unstable KI, LI, SI and TTI values. It is offset by kinematic factors in the form of a weak vertical wind shear (bulk wind shear 0-6 km) and a higher freezing point height. The difference between extreme weather events in the Java Island region and other tropical regions compared to subtropical regions is the strong updraft speed of convection clouds. The IRW Texture COMB algorithm based on the IRW-Texture Gradient modification and the COMB algorithm shows a significant increase in quality for detecting OT and non-OT pixels in Java. Rainy days with rainfall > 50 mm/day are very common in areas with an altitude of > 500 m. On the other hand, the level of convection activity which is indicated by the presence of OT and lightning is significantly reduced in this area. OT of convective clouds appear more frequently during the DJF season and less frequently during the JJA season. OT often appears during the day time and rarely at night time. Most OTs appear in the central part, away from the south and north coasts of Java Island during the day, while OTs that appear near the south and north coasts of Java Island can appear in the morning or afternoon.
Keyword: convective clouds, extreme weather, Overshooting Tops, Himawari 8 Satellite