Judul: Perancangan Primer untuk Pengembangan Sistem Deteksi Berbasis PCR (Polymerase Chain Reaction) pada Ganoderma spp
Abstrak: Ganoderma spp. has been known a pathogenic fungus for palm oil. The aim of this research was to design specific primers and test it to Ganoderma spp. in order to obtain new detection system base on PCR method. Two pairs of primers were designed successfully; Gan-ITS12 primer derived from ITS region and β-Gan primer derivied from β-tubulin region. This two pairs primers were used to identified DNA from mycellia Ganoderma spp. from two different sample sources i.e IPBCC with code 10.658 (GIPB) and three different of Ganoderma sp. from Lombok (Glom1, Glom2, Glom3). The result indicates both Gan-ITS primer and β-Gan primer can amplify Ganoderma spp. on species and genus level respectively. Specifity of primers were tested to DNA from Basidiomycota group and non-Basidiomycota group. The results showed this two pairs primer can not amplification DNA from Basidiomycota group. But, this primers can amplified non-Basidiomycota groups even with more than one band formed.
Keyword: Ganoderma sp., ITS, PCR, β-tubulin

Judul: Identifikasi Ganoderma spp dan Trichoderma spp menggunakan marka molekuler ITS-RFLP
Abstrak: Genetic informations of Ganoderma spp and Trichoderma spp are needed because Ganoderma spp is one of important fungi patogen in estate crops, whereas Trichoderma spp is fungi that can control Ganoderma spp. Research has been conducted to identify the fungi morphologically and physiologically. Due to the low level of accuracy, the use of ITS-RFLP method is expected to identify the species of Trichoderma spp and Ganoderma spp more accurate and specific. The method used are preparation of pure isolates, genomic DNA isolation, amplification by PCR using primers ITS1_ITS4 and ITS5_ITS4, digesting with restriction enzymes AluI, MspI, MluI, and MnlI, and sequencing amplicons. The size of genomic DNA isolated from six samples were about 3000 bp. Trichoderma spp amplicons size were 680 bp, whereas Ganoderma spp amplicons were ranged from 600 to 700 bp. Restriction enzymes that produce the most polymorphic bands pattern are MspI. Samples with different patterns and size of bands indicated a different species, whereas the similar pattern and size of bands shows close relations.
Keyword: Bogor Agricultural University (IPB), Restriction enzyme, PCR, ITS-RFLP, Ganoderma spp, Trichoderma spp

Judul: Distribusi Massa Air Laut Jawa dari Data Hidrografi MAJAFLOX Cruise 2015.
Abstrak: Laut Jawa merupakan perairan dangkal yang dipengaruhi oleh Angin Muson dan Arlindo Selat Makassar. Pemahaman tentang stratifikasi dan distribusi massa air Laut Jawa dapat dilakukan dengan adanya data hidrografi dari MAJAFLOX Cruise pada bulan Agustus 2015. Data hidrografi didapatkan dari penurunan CTD di 14 stasiun pengamatan. Hasil penelitan menunjukkan bahwa stratifikasi vertikal massa air dicirikan oleh lapisan homogen yang kuat dari permukaan hingga dasar perairan. Hal tersebut terjadi akibat percampuran massa air yang kuat oleh aksi gelombang yang dibangkitkan Angin Muson Tenggara. Selain itu, ditemukan variasi spasial dalam arah utara-selatan, dimana karakteristik massa air di tepi selatan dan utara cukup berbeda dengan massa air di tengahnya. Massa air Laut Jawa pada puncak musim timur (Agustus) dicirikan dengan massa air yang hangat (27,18 °C – 28,11 °C), bersalinitas rendah (33,58 psu – 34,2 psu), dan ringan (21,33 kg/m3 – 22,06 kg/m3), kecuali pada stasiun 1 dan stasiun 5 yang cenderung lebih hangat dan lebih tawar dari stasiun lainnya. Pola sirkulasi permukaan menunjukkan salinitas yang relatif tinggi di bagian tengah Laut Jawa dan diduga berhubungan dengan suplai massa air Arlindo Selat Makassar. Variasi tahunan dari analisis keluaran model INDESO menunjukkan adanya stratifikasi massa air yang kuat pada musim peralihan.
Keyword: angin muson, keluaran model INDESO, MAJAFLOX Cruise 2015, pengukuran CTD, stratifikasi massa air