Judul: Penentuan prioritas penghijauan di desa-desa pegunungan Kabupaten Wonosobo
Abstrak: Tujuan penelitian ini adalah untuk nengetahui prioritas lokasi penghijauan yang sebaiknya dilaksanakan di desa-desa pegunungan Kabupaten Wonosabo dengan mengetahui faktor-faktor yang berpengaruh terhadap kerusakan lahan. Untuk tujuan ini telah diteliti 169 desa di Kabupaten Wonosobo yaitu desa-desa yang terletak di atas ketinggian 500 meter dpl. Untuk mengetahui faktor-faktor yang berperanan dalam menduga persamaan erosi, dipergunakan tehnik uji regresi ganda dengan cara regresi bertatar (stepwise) langkah maju (forward selection). Hasil pengujian ini nendapatkan 25 peubah yang masuk dalam persamaan dengan besarnya koefisien determinasi (R2) 70.15 persen. Dari hasil uji persiil ke-25 peubah tersebut terdapat 10 peubah yang nyata. Ke-10 faktor yang berpengaruh nyata terhadap kerusakan lahan rusak adalah (1) lereng lebih dari 40 % (2) lereng antara 15 - 40 % (3) lereng kurang dari 15 % (4) =ah hujan kurang dari 3500 mm/thn (5) curah hujan lebih dari 4000 mm/thn (6) jenis tanah dengan kepekaan erosi sedang (7) penggunaan lahan sawah (8) penggunaan lahan kebun campuran (9) penggunaan lahan perkebunan dan (10) Penggunaan lahan hutan.
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Judul: Pendugaan Tingkat Bahaya Erosi Untuk Optimalisasi Penggunaan Lahan Studi Kasus Di Desa Lamajang, Kecamatan Pangalengan, Kabupaten Bandung, Das Citarum Hulu
Abstrak: Erosion is a major cause of land degradation that lead to critical land formation. Erosion is influenced by several factor, such as : climate, soil erodibility, topograhy, land use, and conservation techniques. Among those five factors the first three factors are factors difficult to change. Therefore, erosion prevention efforts to reduce land degradation generaly focused on the regulation of land use and conservation techniques (land use optimization). Land use optimization was done by allocating any land use for a proper land use in such a way that land degradation can be prevented, i.e land use that produce erosion less than tolerable erosion. Therefore, prediction of actual and potential soil erosion as well as tolerable soil erosion level to determine erosion hazard is necessary. The method used is Universal Soil Loss Equation (USLE) which is a common method used to calculate the erosion rate. The advantages of this method are easy to apply and compatible in areas which have a rainfall and surface runoff as the main factors that causing erosion. The aim of this research is to produce a new scheme of land use based on the level of erosion hazard in order to obtain a sustainable agriculture (optimal allocation of land use) in the Lamajang Village, Sub-district Pangalengan, District of Bandung, West Java. This research includes: field observations, determination of rainfall erosivity, soil erodibility, topography, land use, land conservation techniques, and erosion hazard index. Based on data evaluation and interpretation, 7 land units are found in studied area with erosion rates ranging from low to very high. Land units with erosion hazard greater than tolerance level require land optimization. Optimization of land use activities should include the aspects of erosion, social, cultural, and economic, so make it easy to be accepted and applied for local communities The results of this study showed that erosion hazard in the studied area is very high due to high rainfall and steep slopes. Since actual soil erosion higher than tolerable level it is necessary to optimize the land use. The method of land use optimization suggested for the study area is “Good Terrace”. On areas with slope steepness higher than 60% Agroforestry system is proposed. This Agroforestry system compose of pine as high canopy, coffe or quinine as medium canopy, and chili or tomato as lower canopy (basal cover), that are planted follow contour.
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Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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