Judul: Transformasi Genetik Tanaman Jarak Pagar (Jatropha curcas L.) dengan Gen Metallothionein Tipe 2 dari Melastoma malabathricum melalui Perantara Agrobacterium tumefaciens
Abstrak: The main reason why we use marginal land for non-food crops like jatropha is the limited potential land to cultivate it. The condition of marginal land with low fertility, low pH and high content of heavy-metal can caused inhibit the growth and decreasing production of crop. The tolerance of plants to metal toxicity can be genetically improved by introducing and expressing a gene associated with the metal tolerance. One of these genes is MaMt2, a gene coding type 2 metallothionein that isolated from Melastoma affine. Metallothionein is a cystein-rich protein so it can binding various types of metal. The objective of this research was to obtain increase resistance of jatropha to heavy-metal by integrated MaMt2 gene to jatropha and molecular analysis from transgenic plant. Genetic transformation was performed by using Agrobacterium tumefaciens strain LBA4404 which containing pIG6-SMt2 plasmid. pIG6-SMt2 plasmid contains of MaMt2 gene, hptII gene (hygromycin phosphotransferase) under ubiquitin promoter and NOS (nopaline synthase) terminator. Cotyledon from 2 weeks-old of jatropha cultivar IP-2P was used. The non-transformed explants regenerated 14 days in the non selective regeneration medium while the transformed ones regenerated 18 days in the selective regeneration medium. The average of regenerated shoot each explant was 2.1 for non-transformed explants and 1.6 for transformed ones. Putative transgenic shoots were selected in two step by using medium containing 1.5 mg l-1 and 2.5 mg l-1 of hygromycin successively. This research resulted 25 putative transgenic shoots. Molecular analysis was performed to determine the integration of MaMt2 gene under control for ubiquitin promoter in transgenic shoots by PCR. PCR was carried out by using with UbiQF-NosTR and SMt2F-NosTR combination primers. PCR of transgenic shoots resulted 1160 bp and 526 bp fragments respectively. Molecular analysis showed that three of 15 putative transgenic shoots are transgenic shoots containing MaMt2 gene under control of ubiquitin promoter.
Keyword: 

Judul: Transformasi Jarak Pagar (Jatropha curcas L.) dengan gen MmCuZn-SOD pe nyandi superoksida dismutase melalui perantara Agrobacterium tumefaciens
Abstrak: This research has an objective to ob tain the transgenic Jatropha curcas shoo ts containing MmCuZn-SOD gene. For genetic transfor mation, we used the pieces of cotyledons as an explants. These cotyledons were obtained from 2 week old seedlings. By co-cultivation with Agrobacterium tumefaciens containing MmCuZn-SOD gene under the control of 35S CaMV promoter linked to hygromyc in phosphotrans ferase selectable marker gene. We have successfully regenerated the putative transgenic shoots of J. curcas from the explants. The selection of putative transgenic shoots was carried out by using 1.5 mg/l hygromycin. Molecular analysis by PCR showed that two of sixteen independe nt putative transgenic shoots resulted from indirect selection contain the target gene. These results indicated that we already successfully introduced the MmCuZn-SOD gene into J. curcas and obtained the transgenic adventives shoo t of J. curcas.
Keyword: 

Judul: Infeksi Virus Parainfluenza Pada Anjing
Abstrak: Virus parainfluenza anjing termasuk famili Paramyxoviridae, berbentuk pleomorf dengan ukuran bervariasi (bergaris tengah antara 100-500 nm), berpembungkus peka ether, berisi genom RNA dan secara serologis mempunyai kesamaan dengan virus SV-5 kera; dikenak sebagai salah satu kausa penting dari sindrom "kennel cough". Infeksinya dapat mengenai anjing semua umur yang belum pernah terinfeksi atau mendapatkan vaksinasi mengakibatkan gejala klinis ringan yang terbatas pada traktus respirasi atu infeksi yang secara klinis tidak memperlihatkan gejala nyata. Gejala klinis yang nampak setelah masa inkubasi (1-5 hari) terlewati berupa batuk yang tidak berat selama beberapa hari, berbagai tingkat tonsilitis dan pharyngitis dan kadang-kadang ditemukan kenaikan temperatur rektal antara 1-2 derajat C dari temperatur normal rat-rata. Anjing terinfeksi mengandung virus pada traktus respirasi bagian atas dan bawah serta limfonoduli sampai hari ke 9 setelah infeksi. Penyebaran virus antar anjing terjadi secara aerogen.
Keyword: