Judul: Penggunaan Beberapa Jenis Sitokinin Terhadap Multiplikasi Tunas dan Pertumbuhan Binahong (Anredera cordifolia [Ten.] Steenis) secara In Vitro
Abstrak: Biological diversity in Indonesia occupied third place in the world that using to fulfill needs of basic necessities and supply of medicines. Utilization of medicinal plant in domestic region become larger in a row with improvement of people awareness to consumed natural medicines. Binahong (Anredera cordifolia [Ten.] Steenis) is one of medicinal plant that have a potention to expand as medicine basic commodity. It also useful to treat any diseases and motivated some researchers to examine their bioactive substances. This examination also need research about multiplication using tissue culture technique. Application various of single cytokinin in different concentration is expected able to influenced shoot multiplication and growth of binahong to keep this plant preservation. The research consist of two experiments that first experiment is monitoring effect of sterilization treatment to contamination level, browning and death. Whereas second experiment is monitoring about the effect media treatment in various of cytokinin (AdSO4, BAP, kinetin and thidiazuron) with different concentration (0,50; 1,00; 1,50; and 2,00) mg/l to shoot multiplication and growth of binahong. In second experiment compiled using RAL statistic method with 10 repetations. Parameters are shoot amount (adventif and lateral shoot), height increment, leaf amount, root amount and callus process. In sterilization treatment shows addition of 70 % alcohol during 3 minutes gived highest success percentage (92,76 %). Lowest success percentage happend in control sterilization treatment (46,99 %). Shoot multiplication and growth of binahong shows in 1 MST. This research produced two various of shoots which is adventif shoot ang lateral shoot. Average great amount of shoots produced by 1,50 mg/l kinetin treatment (2,10 adventif shoot) and 1,50 mg/l BAP (3,90 lateral shoot). Control media not produced both of shoots. MS media with addition 0,50 mg/l kinetin showed average height increment and the best amount of leaves are 4,33 cm and 4,70 sheet. In every treatment shows root formation with greatest number produced by control media (8,80 roots). Callus formed in great part of explants that planted in treatment media except in AdSO4 (0,50; 1,00; and 2,00) mg/l and control media. Conclusion of this research is addition various of cytokinin with different concentration in MS media have obvious influental to the parameters.
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Judul: Pengaruh sitokinin (TDZ) dan auksin (IAA dan NAA) terhadap multiplikasi nenas, Ananas comosus L. Merr. cv. Queen dalam perbanyakan kultur jaringan
Abstrak: The objective of this research was to study the effect of Cytokinin (TDZ) and Auxin (IAA and NAA) as growth regulators in shooting multiplication capacity of shoot base pineapple explant. This research was conducted in tissue culture laboratory at Tropical Fruits Study Center. IPB. Baranangsiang. Bogor. since January until September 2004. Planting material of 5-10 mm base shoot of third subculture Queen pincapple's planlet were cultured in shooting induction medium consisted of MS suplemented with Auxin (IAA and NAA) and TDZ in various concentrations. The experiment was set up in factorial design with two factors, Auxin (5 levels: 0 mg/1. 0.1 mg/l IAA, 0.01 mg/l IAA, 0.1 mg/I NAA and 0.01 mg/l NAA) and TDZ (6 levels: 1x10 mg/l. 1x10 mg/l, 1x10 mg/l, 1x10 mg/l, 1x10 mg/l and 1x10 mg/l). There were 30 combinations treatment. Each treatment was consist of 4 replicates, containing 6 explants per replicate, so that found 720 unit. After 20 weeks in shooting induction medium, explants were transferred to MSO medium for shooting multiplication. Each treatment was consist of 12 replicates. In shooting induction phase, the percentage and the the number of shoot. root. nodule and chap nodule were examined. After subculture to MS free medium, the number of axillar shoot, adventitious shoot and chap nodule were examined every weeks. The weight of nodule were examined in the first and last week of observation. Meanwhile. the number of shoot per gram nodule weight were also examined in the last week of observation. The data were statistically analyzed using completely randomized design and the means were compared with Duncan's Multiple Range Test (DMRT) procedure at 5% level of significance. In the first week of the culture shoot induction medium. explants have shown its growing which marked with crosscut leaves elongation and new leaves formation. In almost treatments, shoot started to be formed at 2 weeks after culture (MST) and rooted at 3 MST. except for the treatments of TDZ at concentration 1x10 mg/l. which is combined with various Auxin concentrations. Explants grown in media supplemented with 1x10 mg/l. 1x105 mg/l, 1x10 mg/l. 1x10 mg/l TDZ in different concentrations of Auxin. initially responded by shoot and root formation with no nodules. There were little number of shoots (with amount of mean less than two) that formed in these culture medium by the end of perception week in both phases, shoot induction phase and shoot multiplication phase. Although the shoots cultured in media supplemented with low concentrations of TDZ (below 1x102 mg/l) were fewer in number. but the shoots were longer and have better quality than those formed in media supplemented with high level concentrations of TDZ (above 1x10 mg/) in the same perception week. Shoots became capable of rooting at combinations of 1x10- 1x10 mg/l TDZ with different concentrations of Auxin, but 1x10 mg/l without Auxin gave rise to the best rooting...dst
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Judul: Metrik Einstein (Anti-) Self-Dual Berdimensi Empat dengan Metode Cartan
Abstrak: Gravitational field equation can be formulated in differential forms (differential geometry), which describes gravity as a manifestation of the curvature of space-time. Curvature of space-time is characterized by a metric that satisfies the gravitational field equation. With differential forms it can be formulated the (anti-) self-dual Einstein equation, which is a modification of the Cartan structure in (anti-) self-dual basis. Calculation of (anti-) self-dual metric with (anti-) self-dual Einstein equation is simpler than the calculation using Christoffel symbols.
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