Judul: Identifikasi Metabolit Akar Sidaguri (Sida rhombhifolia L.) Berdasarkan Waktu Tanam Berbeda Menggunakan LC-MS/MS
Abstrak: Sidaguri (Sida rhombifolia L.) merupakan salah satu jenis tanaman yang telah digunakan sebagai obat tradisional dengan berbagai khasiat untuk mengobati banyak penyakit karena adanya kandungan metabolit bioaktif di dalamnya. Kandungan metabolit di dalam akar tanaman sidaguri dipengaruhi oleh beberapa faktor, salah satunya adalah usia tanam. Penelitian ini bertujuan mengidentifikasi senyawa dan mengelompokkan ekstrak akar sidaguri berdasarkan waktu tanam berbeda menggunakan data hasil ekstraksi yang diperoleh dari LC-MS/MS yang dikombinasikan dengan kemometrik. Sebanyak 19 senyawa teridentifikasi dan terkonfirmasi pada ekstrak akar tanaman sidaguri dengan usia tanam 3, 4, dan 5 bulan. Senyawa-senyawa tersebut termasuk ke dalam golongan fenolik, alkaloid, steroid, flavonoid, kumarin, asam benzoat dan asam lemak. Berdasarkan hasil pengelompokkan dengan PCA menggunakan variabel luas puncak 19 senyawa metabolit yang teridentifikasi, ekstrak sidaguri mengelompok sesuai usia tanam masing-masing dengan total plot skor sebesar 68%., Sidaguri (Sida rhombifolia L.) is a type of plant that has been used as a traditional medicine with various properties for treating many diseases because of the bioactive metabolites it contains. The metabolites content in the roots of the sidaguri were affected by some factors, such as cultivation age. This research aims to identify compounds and classify sidaguri root extracts based on different planting times using extraction data obtained from LC-MS/MS combined with chemometrics.. A total of 19 compounds were identified and confirmed in the root extract of the sidaguri plant with a planting age of 3, 4 and 5 months. These compounds belong to the phenolic, alkaloids, steroids, flavonoids, coumarins, benzoic acid and fatty acids groups. Based on the results of grouping using PCA using the peak area variable of 19 identified metabolite compounds, sidaguri extracts were grouped according to their respective planting ages with a total score plot of 68%.
Keyword: metabolit, metabolit sekunder

Judul: Metabolite Profiling of Leaves Extracts of Sida rhombifolia L. with Different Planting Age Using Liquid Chromatography-Mass Spectrometry
Abstrak: Sidaguri (Sida rhombifolia L.) adalah tanaman obat yang diyakini khasiatnya oleh masyarakat sebagai obat asam urat, antihipertensi, dan lain sebagainya. Penelitian ini bertujuan mengidentifikasi komposisi metabolit bioaktif daun sidaguri. Analisis ekstrak daun sidaguri diklasifikasikan berdasarkan umur tanam tiga, empat, dan lima bulan menggunakan kromatografi cair-spektrometri massa (LC-MS/MS) dan dikelompokan menggunakan analisis komponen utama (PCA). Kromatogram LC-MS/MS diolah menggunakan perangkat lunak Thermo Scientific Xcalibur 4.2 dan Compound Discoverer 3.2. Analisis multivariat menggunakan perangkat lunak The Unscrambler X 10.4. Sebanyak 36 metabolit berhasil teridentifikasi dalam ekstrak. Metabolit tersebut termasuk dalam golongan alkaloid, asam fenolat, flavonoid, kumarin, steroid, dan golongan lain. Ekstrak daun sidaguri terkelompok ke dalam kelasnya masing-masing, yaitu umur tanam 3, 4, dan 5 bulan dengan total varians 77%., Sida rhombifolia L. (sidaguri) is a medicinal plant that is believed to be useful by the community as a gout drug, antihypertensive, and so on. This study aims to identify the composition of bioactive metabolites of sidaguri leaves. Analysis of sidaguri leaf extract was classified based on the planting age of three, four, and five months using liquid chromatography-mass spectrometry (LC-MS/MS) and grouped using principal component analysis (PCA). The LC-MS/MS chromatograms were processed using Thermo Scientific Xcalibur 4.2 and Compound Discoverer 3.2 software. Multivariate analysis using The Unscrambler X 10.4 software. A total of 36 metabolites were identified in the extract. These metabolites are included in the group of alkaloids, phenolic acids, flavonoids, coumarins, steroids, and other groups. Sidaguri leaf extracts were grouped into their respective classes, namely 3, 4, and 5 months of planting age with a total variance of 77%.
Keyword: LC-MS/MS, metabolit sekunder, PCA, Sida rhombifolia L., LC-MS/MS, PCA, Sida rhombifolia L., secondary metabolites

Judul: Dynamical System of Hepatitis B in Three Groups with Vaccination
Abstrak: The aim of this study is to modify the hepatitis B transmission model in three age groups involving vaccination from the model conducted by Zhoe and Zhang.The method used is to analyze the stability of the fixed point and its basic reproduction number (R_0). Performed stability analysis and numerical simulation to see population dynamics. Next, a sensitivity analysis of the parameters was carried out to see its effect on R_0. From the analysis obtained two fixed points, namely non-endemic and endemic fixed points. Non-endemic fixed points are locally asymptotically stable if R_0 <1 Meanwhile, the endemic fixed point is locally asymptotically stable if R_0 > 1. Hepatitis vaccination affects the number of susceptible population, because the more successful the vaccination, the smaller the number of susceptible populations so that the smaller the population will be infected.
Keyword: Dynamic, Hepatitis B, Vaccine, Mathematical model