Judul: Analysis of City Parks Ecological Function for Central Jakarta Sustainability
Abstrak: Taman Kota sebagai ruang terbuka hijau, memiliki fungsi ekologis yang berperan dalam mendukung keberlanjutan kota. Jakarta Pusat memiliki beberapa taman kota yang berpotensi mendukung keberlanjutan kota jika berfungsi dengan optimal. Penelitian ini bertujuan menganalisis fungsi ekologis dan persepsi masyarakat terhadap fungsi ekologis di Taman Menteng, Taman Situ Lembang, dan Taman Suropati. Fungsi ekologis dinilai dengan membandingkan kondisi eksisting dengan kriteria yang telah ditetapkan, dan persepsi masyarakat didapatkan melalui kuesioner. Hasil penelitian menunjukkan ketiga taman memiliki fungsi yang berjalan cukup baik, namun masih perlu peningkatan dalam beberapa fungsi. Mayoritas responden menganggap fungsi sudah berjalan baik, namun mengharapkan beberapa fungsi dapat ditingkatkan., City parks, as green open spaces, serve an ecological function that plays a crucial role in supporting urban sustainability. In Central Jakarta, a few city parks have the potential to contribute significantly to the city's sustainability if their ecological functions are optimized. This research aims to analyze the ecological functions and public perceptions regarding these functions in Taman Menteng, Taman Situ Lembang, and Taman Suropati. Ecological functions assessed by comparing the current condition of each tree against established criteria, and public perceptions gathered through questionnaire data. The results indicate that each park is functioning adequately, but there is room for improvement in certain ecological functions. The majority of respondents acknowledge that the functions are performing well; however, they express a desire for some aspects of the functions to be improved.
Keyword: City Park, Ecological Function, Green Open Space, City Sustainability

Judul: Analysis of City Parks Ecological Function for Tangerang City Sustainability
Abstrak: Taman kota sebagai salah satu jenis ruang terbuka hijau publik memiliki peran penting dalam meningkatkan kualitas lingkungan dan mendukung keberlanjutan perkotaan. Untuk mengetahui sejauh mana implementasi fungsi ekologis, diperlukan analisis terhadap fungsi-fungsi tersebut. Penelitian ini bertujuan untuk menganalisis kesesuaian fungsi ekologis dan persepsi dan preferensi masyarakat terhadap fungsi ekologis taman kota di Kota Tangerang. Penelitian dilakukan di tiga taman yang ada di Kota Tangerang yaitu Eco Park, Taman Pramuka, dan Taman Gajah Tunggal. Metodologi penelitian terdiri dari dua metode yaitu analisis fungsi ekologis dan analisis persepsi dan preferensi masyarakat di masing-masing taman. Fungsi ekologis dianalisis dengan membandingkan kondisi eksisting dengan kriteria standar, sedangkan analisis sosial dilakukan melalui pengumpulan data menggunakan kuesioner. Hasil analisis ekologi menunjukkan bahwa Eco Park unggul dalam fungsi modifikasi suhu. Taman Pramuka memiliki vegetasi yang berfungsi baik sebagai penahan angin dan penahan erosi. Taman Gajah Tunggal memiliki fungsi peredam kebisingan terbaik. Melalui penyebaran kuisioner diketahui bahwa mayoritas masyarakat merasa puas dengan kondisi ketiga taman tersebut., Urban city parks, as a type of public green open space, play an important role in improving the quality of the urban environment and supporting urban sustainability. To determine the extent of ecological function implementation, an analysis of these aspects is necessary. This research aims to analyze the suitability of ecological functions and examine public perceptions and preferences regarding the ecological functions of city parks in Tangerang City. The study was conducted in three parks in Tangerang City, namely Eco Park, Pramuka Park, and Gajah Tunggal Park. The research methodology consists of two methods: ecological function analysis and analysis of public perceptions and preferences in each park. Ecological functions are analyzed by comparing the existing conditions with standard criteria, while social analysis is conducted through data collection using questionnaires. The results of the ecological analysis show that Eco Park excels in temperature modification function. Pramuka Park has vegetation that adequately serves as windbreak and erosion control. Gajah Tunggal Park performs the best in noise reduction function. Through the distribution of questionnaires, it was found that the majority of the community is satisfied with the conditions of all three parks.
Keyword: city park, ecological function, green open space, urban sustainability

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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