Judul: Stability and Estimation of Shelf Life of Tuna Eye Fish Oil-Based Emulsion Products and Katsuobushi Protein Hydrolyzate
Abstrak: Docosahexaenoic Acid (DHA) sangat dibutuhkan pada masa kehamilan dan laktasi untuk perkembangan otak janin (bayi), dan dapat dikonsumsi sebagai suplemen untuk anak-anak serta orang dewasa dalam bentuk kapsul lunak maupun produk emulsi cair. Formulasi produk emulsi suplemen minyak ikan mata tuna kaya DHA dan hidrolisat protein ikan kayu masih diperlukan terkait kendala stabilitas emulsi dan umur simpan. Penelitian ini bertujuan menentukan stabilitas dan umur simpan produk emulsi berbasis minyak ikan mata tuna dan hidrolisat protein ikan kayu. Penelitian meliputi produksi minyak ikan mata tuna, pembuatan hidrolisat protein ikan kayu dengan enzim papain 3.000 unit/g, pembuatan produk emulsi minyak ikan mata tuna dan hidrolisat protein ikan kayu, penyimpanan selama 14 hari, dan pendugaan umur simpan dengan metode Arrhenius pada suhu 40°C, 45°C, dan 50°C menggunakan titik kritis bilangan peroksida. Stabilitas emulsi menunjukkan adanya penurunan selama penyimpanan yang ditandai dengan peningkatan ukuran globula dari 1 µm menjadi 3 µm dan terjadi agregasi serta koalesensi pada penyimpanan hari ke 8 dan ke 10. Umur simpan melalui pendugaan Arrhenius menggunakan ordo nol dihasilkan lama penyimpanan 144 hari atau sekitar 4 bulan pada suhu 29°C., Docosahexaenoic Acid (DHA) is necessary during pregnancy and lactation for fetal brain development (infants), and can be consumed as a supplement for children and adults in the form of soft capsules or liquid emulsion products. The formulation of a DHA-rich tuna eye fish oil supplement emulsion product and katsuobushi protein hydrolyzate is still desired due to the constraints of emulsion stability and shelf life. This objective of this study were to determine the stability and shelf life of tuna eye fish oil-based emulsion products and katsuobushi protein hydrolyzate. The research included the production of tuna eye fish oil, manufacture of katsuobushi protein hydrolyzate with papain enzyme 3,000 units/g, manufacture of tuna eye fish oil emulsion and katsuobushi protein hydrolyzate, storage for 14 days, and estimation of shelf life using the Arrhenius method at 40° C, 45°C, and 50°C with the critical point of peroxide number. Emulsion stability showed a decrease during storage which was indicated by an increase in globule size from 1 m to 3 m and the aggregation and coalescence occurred on the 8th and 10th days of storage. Shelf life through Arrhenius estimation using zero order resulted in a storage period of 144 days or about 4 months at 29°C.
Keyword: Arrhenius, DHA, Stunting

Judul: Pregnancy Supplement Formulation Based on Tuna Eyes Virgin Fish Oil Protected by Antioxidant Secang Wood with the Mixture Design
Abstrak: Indonesia menghadapi tantangan besar terkait prevalensi stunting, sehingga dari aspek gizi, pemenuhan docosahexaenoic acid (DHA) menjadi penting dalam pencegahan dampak penurunan kecerdasan akibat stunting. Docosahexaenoic acid dapat menjadi model suplementasi omega-3 untuk perkembangan otak dan retina mata. Mata tuna mengandung DHA yang tinggi, namun fortifikasi minyak ikan dalam produk pangan rentan terhadap oksidasi sehingga membutuhkan antioksidan. Kayu secang mengindikasikan memiliki aktivitas antioksidan yang tinggi. Penelitian ini bertujuan menentukan optimasi formulasi suplemen kehamilan berbasis virgin fish oil mata tuna terproteksi antioksidan kayu secang menggunakan mixture design. Penelitian meliputi determinasi karakteristik kayu secang, karakteristik mata tuna, cold centrifugation separation, karakteristik virgin fish oil mata tuna dan optimasi formulasi dengan mixture design. Hasil penelitian menunjukkan mata tuna memiliki kandungan DHA sebesar 32,12%. Kayu secang memiliki aktivitas antioksidan 5,08±0,21 ppm dan nilai total fenol 2165,80±0,14 mg GAE/g. Formula optimal didapatkan dari variabel virgin fish oil mata tuna 39,98%, kayu secang 21,68% dan guar gum 38,33% dengan inhibisi bilangan peroksida 63,72%. Nilai respon optimal pH dan viskositas 3,10 dan 478,76 cPs, sedangkan verifikasi di laboratorium 3,27±0,9 dan 479±2,83 cPs., Indonesia faces major challenges related to the prevalence of stunting, so from nutritional aspect, the fulfillment of docosahexaenoic acid (DHA) is important in preventing the decline in intelligence due to stunting. Docosahexaenoic acid (DHA) can be a model for omega-3 supplementation for the development of the brain and retina of the eye. Tuna eyes contain high DHA. However, fortification of fish oil in food products is susceptible to oxidation and thus requires antioxidants. Secang wood has high antioxidant activity. The study aims to determine the optimization of a pregnancy supplement formulation based on tuna eyes virgin fish oil protected antioxidant secang wood with the mixture design. Research covering determination of the characterisctics of secang wood, characterisctics of tuna eyes, cold centrifugation separation, characterisctics of tuna eyes virgin fish oil and formulation optimizing with mixture design. The results showed that tuna eyes had a DHA content of 32,12%. Secang wood has an antioxidant activity of 5,08±0,21 ppm and a total phenol value of 2165,80±0,14 mg GAE/g. The optimal formula obtained was 39,98% tune eyes virgin fish oil, secang wood 21,68% and guar gum 38,33% with 63,72% inhibition of peroxide value. The optimal pH and viscosity response values were 3,10 and 478,76 cPs, while the laboratory results were 3,27±0,9 and 479±2,83 cPs.
Keyword: Kayu Secang, stunting, tuna, virgin fish oil, secang wood, stunting, tuna, virgin fish oil

Judul: Etno-ornitologi Burung Kancilan Flores (Pachycephala nudigula nudigula Hartert 1897) pada Masyarakat Lio di Taman Nasional Kelimutu
Abstrak: Bare-throathed whistler is one of several wild birds in Kelimutu National Park (TNKL) that is believed to have correlation with Lio Community‟s belief. The estimation of Bare-throated whistler‟s population in Kelimutu National Park was about 186.41-1668.07 individual by the density about 0.0389-0.3481 ind/ha with the sex ratio 6.2:1 that spreaded on area with height of 1100 – 1600 masl. The characteristic of Bare-throated whistler‟s habitat is a natural forest with various canopy level, high plant species diversity and high density. The habitat characteristics related to the availability of food in the form of insects, cover, safety and a nesting site. Generally the most of Lio community knew the existence of Bare-throated whistler directly or from their parents‟ stories. The perceptions of Lio Community to the Bare-throated whistler‟s existence is generally support the conservation efforts. The perceptions construct the Lio Community‟s atitude that tend to be conservative to the existence of Bare-throated whistler. The attitude shown by their behavior that not cathing the Bare-throated whistler from the TNKL‟s area or the outside. The attitude have an important role to the existence of Bare-throated whistler in Kelimutu National Park‟s area.
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