Judul: Bioactive Profile Analysis and Inhibitory Activity of Prostaglandin Synthase of Sungkai Leaf Extract (Peronema canescens Jack) In Silico
Abstrak: Daun sungkai (Peronema canescens Jack) memiliki sifat antipiretik yang telah dibuktikan secara etnobiologi. Namun, penelitian seputar daun sungkai masih sangat minim, terutama dalam bidang in silico maupun in vitro. Penelitian ini bertujuan untuk membuktikan kemampuan daun sungkai untuk mempercepat proses penurunan suhu tubuh saat demam. Daun sungkai diekstraksi menggunakan pelarut etanol 70%. Hasil ekstraksi dipekatkan menggunakan rotary evaporator, lalu dianalisis menggunakan perangkat LC-MS. Senyawa yang didapat dari LC-MS dianalisis secara in silico menggunakan kriteria Rules of 5 Lipinski dan ADMET. Senyawa yang berhasil lolos skrining ditambatkan dengan enzim target mPGES-1 menggunakan aplikasi Yasara Structure. Ekstraksi dengan pelarut etanol 70% menghasilkan rendemen ekstrak sebesar 14,75%. Hasil analisis LC-MS menunjukkan keberadaan 22 senyawa yang diketahui dan satu senyawa Unknown. Hasil skrining menujukkan sepuluh senyawa berhasil lolos. Simpulan dari penelitian ini adalah senyawa 2-Oxo-6-(piperidine-1-sulfonyl)-benzooxazole-3-carboxylic acid isobutyl ester; 2-(5-{[Isopropyl(methyl) amino]methyl}-1-tetrazolidinyl)-N-methylN-[2-(2-methyl-1-imidazolidinyl)ethyl]acetamide;2-({2-[4-(2-Hydroxyethyl)-1-piperazinyl]-2-oxoethyl}sulfanyl)- 5,6-dimethylthieno [2,3-d] pyrimidin -4(3H)-one;2-({4-Amino-5-[3-(diethylsulfamoyl)phenyl]-4H-1,2,4-triazol-3-yl}sulfanyl)-N-methyl Acetamide; dan 3,4,5-Trimethoxycinnamate memiliki kemampuan inhibisi mPGES-1 berdasarkan binding energy dan konstanta inhibisi yang dimiliki tiap ligan uji., Sungkai leaves (Peronema canescens Jack) have antipyretic properties that have been ethnobiologically proven. However, research on sungkai leaves is very minimal, especially in the field of in silico and in vitro. This study aims to prove the ability of sungkai leaves to accelerate the process of decreasing body temperature during fever. Sungkai leaves were extracted using a 70% ethanol solvent. The extraction results were concentrated using rotary evaporator, then analyzed using an LC-MS device. Compounds obtained from LC-MS were analyzed in silico using Lipinski's Rules of 5 criteria and ADMET. Compounds that successfully passed the screening were docked with the target enzyme mPGES-1 using the Yasara Structure application. Extraction with 70% ethanol solvent resulted in an extract yield of 13.1570%. The results of the LC-MS analysis showed the presence of 22 known compounds and one Unknown compound. The screening results showed that ten compounds had successfully passed. The conclusion of this study is compound 2-Oxo-6-(piperidine-1-sulfonyl)-benzooxazole-3-carboxylicacid isobutyl ester; 2-(5-{[Isopropyl(methyl)amino]methyl}-1-tetrazolidinyl)-N-methyl N-[2-(2-methyl-1-imidazolidinyl)ethyl]acetamide; 2-({2-[4-(2-Hydroxyethyl)-1-piperazinyl]-2-oxoethyl}sulfanyl)-5,6-dimethylthieno [2,3-d] pyrimidin -4(3H)-one; 2-({4-Amino-5-[3-(diethylsulfamoyl)phenyl]-4H-1,2,4-triazol-3-yl}sulfanyl)-N-methyl Acetamide; and 3,4,5-Trimethoxycinnamate have the ability to inhibit mPGES-1 based on the binding energy and inhibition constant that each test ligand has.
Keyword: Bioaktif, Demam, LC-MS, Molecular Docking, Sungkai

Judul: Potensi Senyawa Aktif Sungkai (Peroema canescens) dalam Menginhibisi Enzim Beta-Laktamase Secara In Silico.
Abstrak: Kasus resistensi bakteri terhadap antibiotik menjadi ancaman serius bagi kesehatan manusia karena menurunkan efektivitas obat untuk mencegah dan menyembuhkan infeksi bakteri. Resistensi dapat disebabkan oleh pengobatan yang tidak tuntas. Salah satunya yaitu bakteri Escherichia coli yang menghasilkan enzim CTX-M yang merupakan jenis dari beta-laktamase yang menghambat kerja antibiotik golongan beta-laktam. Sungkai merupakan salah satu tanaman obat tradisional dan terbukti menghambat pertumbuhan bakteri Escherichia coli. Penelitian ini bertujuan mengidentifikasi senyawa aktif sungkai yang berpotensi menginhibisi beta-laktamase serta analisis interaksi yang terjadi secara in silico. Senyawa aktif daun sungkai ditambatkan ke reseptor beta-laktamase, kemudian dianalisis interaksinya serta diprediksi bioavailabilitas dan toksisitas. Hasil penelitian menunjukan sebanyak 6 dari 19 ligan uji lolos penapisan hingga akhir dan berpotensi menjadi inhibitor beta-laktamase. Senyawa tersebut antara lain, beta-sitosterol, 3,4’,5-thrihydroxy-3,’,7-dimenthoxyflavanone, physcion, isorhamnetin, chrysoeriol, quercetin 3,3 dimetly ether, dan vintexin-2-O rhamnoside., Cases of bacterial resistance to antibiotic has become a serious threat for human health because they reduce the effectiveness of drugs in preventing and treating bacterial infections. Resistance can be caused by uncomplete treatment. One of example is Escherichia coli bacteria which produces the CTX-M enzyme, that is a type of of beta-lactamase that inhibits the action of beta-lactam antibiotics. Sungkai is one of the traditional medicinal PLANTS and it is proved inhibiting the growth of Escherichia coli. This research aims to identify the active compounds of sungkai that have the potential to inhibits beta-lactamase and analyze interactions of molecular docking. The active compounds of sungkai leaves are docked to the beta-lactamase receptor, then their interactions are analyzed, predicting bioavailability, and toxicity. The results showed that 6 of the 19 tested ligands pass the screening and have the potential to become beta-lactamase inhibitors. The compounds are beta-sitosterol, 3,4’,5-thrihydroxy-3’7-dimenthoxyflavanone, physcion, isorhmaetin, chrysoeriol, quercetin 3,3 dimetly ether, and vintexin-2-O-rhamnoside.
Keyword: resistent, beta-lactamase inhibitors, sungkai

Judul: Penentuan Nilai Eigen dan Vektor Eigen dari Matriks Tridiagonal 2-Toeplitz dengan Pendekatan Polinomial Chebyshev
Abstrak: The eigenvalues and eigenvectors of a matrix can be determined by finding its characteristic polynomials. The characteristic polynomials of a tridiagonal 2-Toeplitz matrix is shown to be closely connected to polynomials which satisfy the Chebyshev recurrence relationship. If the order of the matrix is odd, then the eigenvalues are found explicitly in terms of the Chebyshev zeros and the eigenvectors are found in terms of the polynomials satisfying the recurrence relationship. For even ordered matrices, the situation is more complicated. The problem in these cases is that although the Chebyshev recurrence formula is still applied, its initial values are not generating Chebyshev polynomials
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