Judul: Analysis of ecological function of urban green open space for Bekasi's sustainability
Abstrak: Penelitian ini dilakukan untuk menganalisis kesesuaian fungsi ekologis dan persepsi masyarakat terhadap peran taman kota bagi keberlanjutan Kota Bekasi. Penelitian difokuskan pada tiga RTH yaitu Taman Kota Bekasi, Taman Pemerintah Kota Bekasi dan Hutan Kota Patriot Bina Bangsa yang berada di Kota Bekasi dengan luas masing-masing 1,19 Ha, 6,80 Ha dan 6,50 Ha. Penelitian ini dilakukan dengan tahapan persiapan, inventarisasi, analisis dan sintesis. Pengumpulan data dilakukan melalui observasi lapangan, wawancara dengan pengelola, studi literatur dan penyebaran kuesioner. Diperoleh taman-taman di Kota Bekasi sesuai terhadap fungsi ekologisnya karena dapat mereduksi kebisingan sebesar 16,03 dBA, menurunkan suhu sebesar 3,01°C, dan mengendalikan kelembaban sebesar 3,22%. Mayoritas responden sadar akan keberlanjutan dan peran mereka dalam mencapai keberlanjutan tersebut, juga sadar akan tindakannya dalam menjaga lingkungan dan berperan penting dalam keberlanjutan Kota Bekasi. Hal tersebut perlu mendapat dukungan pemerintah dalam pengembangan fasilitas dan utilitas serta sarana dan prasarana yang ada seperti taman kota yang ada di sekitar Kota Bekasi., This research aimed to analyze the ecological functions and public perceptions of urban parks in relation to the sustainability of Bekasi City. The research focused on three green spaces, namely Bekasi City Park, Bekasi City Government Park and Patriot Bina Bangsa Urban Forest in Bekasi City with an area of 1,19 Ha, 6,80 Ha and 6,50 Ha respectively. This research was conducted with the stages of preparation, inventory, analysis and synthesis. Data was collected through field observations, interviews with managers, literature studies and distributing questionnaires. The results show that the parks in Bekasi City fulfill their ecological functions as they can decrease noise levels by 16,03 dBA, lower temperature by 3,01°C, and control humidity by 3,22%. The majority of respondents are aware of sustainability and their contribution towards its attainment. Additionally, they acknowledge their environmental preservation efforts and pivotal role in the sustainability of Bekasi City. This needs government support in the development of facilities and utilities as well as existing facilities and infrastructure such as city parks around Bekasi City.
Keyword: Analisis, Keberlanjutan, Ruang terbuka hijau, green open space, sustainability

Judul: Analysis of City Parks Ecological Function for Central Jakarta Sustainability
Abstrak: Taman Kota sebagai ruang terbuka hijau, memiliki fungsi ekologis yang berperan dalam mendukung keberlanjutan kota. Jakarta Pusat memiliki beberapa taman kota yang berpotensi mendukung keberlanjutan kota jika berfungsi dengan optimal. Penelitian ini bertujuan menganalisis fungsi ekologis dan persepsi masyarakat terhadap fungsi ekologis di Taman Menteng, Taman Situ Lembang, dan Taman Suropati. Fungsi ekologis dinilai dengan membandingkan kondisi eksisting dengan kriteria yang telah ditetapkan, dan persepsi masyarakat didapatkan melalui kuesioner. Hasil penelitian menunjukkan ketiga taman memiliki fungsi yang berjalan cukup baik, namun masih perlu peningkatan dalam beberapa fungsi. Mayoritas responden menganggap fungsi sudah berjalan baik, namun mengharapkan beberapa fungsi dapat ditingkatkan., City parks, as green open spaces, serve an ecological function that plays a crucial role in supporting urban sustainability. In Central Jakarta, a few city parks have the potential to contribute significantly to the city's sustainability if their ecological functions are optimized. This research aims to analyze the ecological functions and public perceptions regarding these functions in Taman Menteng, Taman Situ Lembang, and Taman Suropati. Ecological functions assessed by comparing the current condition of each tree against established criteria, and public perceptions gathered through questionnaire data. The results indicate that each park is functioning adequately, but there is room for improvement in certain ecological functions. The majority of respondents acknowledge that the functions are performing well; however, they express a desire for some aspects of the functions to be improved.
Keyword: City Park, Ecological Function, Green Open Space, City Sustainability

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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