Judul: Pengaruh Interaksi Orang Tua-Anak dan Dukungan Sosial terhadap Insecurity Remaja Madya
Abstrak: Insecurity merupakan permasalahan yang marak terjadi pada remaja di Indonesia. Insecure merupakan pandangan kurang damai atau ketidaknyamanan yang dialami oleh individu. Karakteristik insecure yang sering terjadi adalah tidak percaya diri, kecemasan, khawatir terhadap masa depan, dan perasaan kurang dalam berbagai hal. Orang tua dan lingkungan sosial berperan penting dalam pencegahan insecurity pada remaja. Penelitian ini bertujuan untuk menganalisis pengaruh interaksi orang tua-anak dan dukungan sosial terhadap tingkat insecurity remaja madya. Penelitian ini menggunakan desain penelitian explanatory. Teknik penarikan sampel menggunakan metode non-probablity sampling dengan teknik voluntary sampling. Jumlah sampel sebanyak 202 responden siswa SMA berusia 16-18 tahun dan berdomisili di Jabodetabek. Hasil penelitian menunjukkan hubungan negatif antara pendidikan orang tua, interaksi orang tua-anak dan dukungan sosial dengan insecurity remaja. Terdapat pengaruh negatif signifikan antara pendidikan ibu, interaksi orang tua-anak dan dukungan sosial terhadap insecurity remaja. Berdasarkan penelitian ini interaksi orang tua-anak dan dukungan sosial yang baik akan menurunkan tingkat insecurity pada remaja., Insecurity is a problem that is currently happening among adolescent in Indonesia. Insecure is a lack of peace feeling or discomfort experienced by individuals. Insecure characteristics are lack of self confidence, anxiety, overthink about future, and feelings of lack in everything. Parents and social environment around adolescent play an important role in preventing insecurity. This study aims to analyze the effect of parent-child interaction and social support on insecurity among adolescent. This study used an explanatory research design. The sampling technique used a non-probablity sampling method with a voluntary sampling technique. The sample size was 202 high school student respondents aged 16-18 years in Jabodetabek. The results showed negative relationship between parent education, parent-child interaction and social support with adolescent insecurity. There was a significant negative effect between mother’s education, parent-child interaction and social support with adolescent insecurity. Based on this study, a good parent-child interaction and social support will reduce the level of insecurity in adolescents.
Keyword: adolescents, insecurity, parent-child interaction, social support

Judul: The Effect of Social Support and Adolescent-Parents Communication on Subjective Well-Being of Adolescent among Indonesian Migrant Worker Families
Abstrak: Kesejahteraan subjektif adalah persepsi seseorang mengenai kebahagiaan dan kepuasan terhadap pengalaman hidupnya. Tujuan penelitian ini adalah menganalisis karakteristik remaja, karakteristik keluarga, pengaruh dukungan sosial, komunikasi remaja-orang tua terhadap kesejahteraan subjektif remaja pada keluarga pekerja migran. Metode penelitian ini menggunakan metode non-probability sampling dengan teknik convenience sampling di lima sekolah terpilih di wilayah Kecamatan Kesugihan, Kabupaten Cilacap, Provinsi Jawa Tengah. Responden penelitian ini melibatkan 99 remaja SMP sederajat terdiri atas 54 persen perempuan dan 46 persen laki-laki dengan usia berkisar 12-16 tahun. Hasil penelitian menunjukkan bahwa dukungan sosial tertinggi yang didapatkan remaja diperoleh dari dukungan sosial orang tua. Komunikasi remaja dengan ibu lebih baik dibandingkan komunikasi ayah dengan remaja. Hasil uji korelasi menyatakan dukungan sosial dan komunikasi remaja-orang tua mempunyai hubungan positif signifikan dengan kesejahteraan subjektif remaja. Hasil penelitian menemukan jenis kelamin berpengaruh negatif signifikan terhadap kesejahteraan subjektif remaja. Dukungan sosial komposit berpengaruh positif terhadap kesejahteraan subjektif remaja. Komunikasi ibu berpengaruh positif signifikan terhadap kesejahteraan subjektif remaja., Subjective well-being is the perception of happiness and satisfaction with life experiences from someone's point of view. The purpose of this research is analyze the effect of adolescent characteristics, family characteristics, social support, and adolescent-parental communication on the subjective well-being of adolescents in migrant worker families. The Method used in this research is non-probability sampling method with a convenience sampling technique in five selected schools in the Kesugihan District, Cilacap Regency, Central Java. The respondents of this study involved 99 junior high school students with ages ranging from 12-16 years. The highest social support is received from parents. The result of the study also found that mother communication with adolescent is better than father communication with adolescent. The result shows that social support and child-parent communication has a significant positive correlation with adolescents subjective well-being. The results of the study suggest that gender had a significant negative effect on the subjective well-being of adolescents. Composite social support has a positive effect on the subjective well-being of adolescents. Mother's communication has a significant positive effect on the subjective well-being of adolescents.
Keyword: dukungan sosial, keluarga pekerja migran, kesejahteraan subjektif, komunikasi remaja-orang tua

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable