Judul: Rainfall Behavior in some Area Over Indonesia When Monsoon and Dipole Mode Phenomenon have Interaction
Abstrak: Penelitian ini didasarkan pada Monsun sebagai salah satu fenomena iklim regional. Selain itu Indian Ocean Dipole merupakan fenomena couple antara atmosfer dan laut yang memiliki peran penting dalam variabilitas curah hujan di Indonesia. Menurut itu, studi ini dilakukan dengan asumsi ketika Monsun dan IOD berinteraksi. Kajian wilayah studi curah hujan meliputi Lampung, Pontianak, Indramayu dan Banjar Baru untuk periode Januari 1998 – Agustus 2010 dengan menggunakan data TRMM 3B43. Data indeks monsun yang digunakan adalah ISMI (Indian Summer Monsoon Index), WNPMI (Western North Pacific Monsoon Index) dan AUSMI (Australian Monsoon Index). Hasil Power Spektral Density (PSD) dan Analisis Wavelet pada anomali curah hujan dan data indeks monsun menunjukkan pola osilasi dominan 12 bulanan dimana curah hujan dan sinyal monsun yang kuat. Sedangkan pola osilasi dominan IOD sekitar 38 bulan. Hasil analisis regresi menunjukkan hubungan yang signifikan antara anomali curah hujan dengan data AUSMI. Oleh karena itu, AUSMI dan IOD dapat digunakan untuk menganalisis varibialitas curah hujan di Indonesia. Dalam studi ini, model prediksi fenomena interkoneksi antara AUSMI dan IOD dikembangkan dengan menggunakan metode regresi multivariat dengan model awal masing-masing wilayah kajian Lampung Yt = 0.153Xt + 0.066Xt-1 + 0.021Xt-2 + 0.79901, Pontianak Yt = 0.053Xt-1 + 0.011Xt-2 – 0.076Xt-3 + 1.33557, Banjarbaru Yt = 0.108Xt + 0.098Xt-1 – 0.006Xt-2 + 1.10343, dan Indramayu Yt = 0.162Xt + 0.122Xt-1 – 0.004Xt-2 + 0.94325. Metode Box-Jenkins berdasarkan ARIMA (Autoregressive Integrated Moving Average) digunakan untuk prediksi data deret waktu anomali curah hujan untuk Lampung adalah ARMA (2,2)4 dengan pesamaan Zt = -0,0245Zt-12 - 0,9711Zt-24 + at -0,0013at-12 + 0,8560at-24. Model prediksi untuk Pontianak adalah ARMA (2,2)12 dengan Zt = 0.0319Zt-12 + 0.96171Zt-24 + at + 0.0388at -12 + 0.8428at-24, Banjar baru adalah ARMA (1,1)12 dengan Zt = 0.9967Zt-12 + at + 0.8967at-12, dan Indramayu adalah ARMA (1,1)12 with Zt = -1,0037Zt-12 + at + 0,9363at-12 Berdasarkan dari nilai koefisien korelasi, Lampung dan Indramayu memiliki model prediksi curah hujan yang baik.
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Judul: Pengembangan Model Indeks Monsun (Monsoon) Indonesia (IMI) Berbasis Hasil Analisis Data WPR (Wind Profile Radar)
Abstrak: There are three regions as a heat source to drive a circulation in the equatorial namely South America, Africa, and Indonesian. Indonesian in a geographyc is a unique country. Because in the cross two continental (Asia continental and Australian continental) and two ocean (Hindian ocean and Pacific ocean). Until Indonesian climate is dominated by Monsoon to have one year of cycle. Development of model in one of purpose to easier in predicted climate in the future is seldom done. So throught the research are model can be used Monsoon prediction especially in Indonesia. This research to used a WPR in three city, Pontianak, Manado, and Biak with supporting data is global climate that ISMI (Indian Summer Monsoon Index), WNPMI (Western North Pacific Monsoon Index), and AUSMI (Australian Monsoon Index). The results of this research are the linkage between the WPR with global climate data, so AUSMI data to selected which oscillate around 12 months. The powerfull of correlation in the fist period (November 1, 2007 – January 30, 2008) is AUSMI in Pontianak and Biak with the correltion 0.486 and 0.599. whereas second period (Desember 7, 2008 – March 7, 2009) AUSMI dominant in Pontianak with correlation value 0.21 and ISMI dominant in Biak with correlation value 0.419. Furthermore with bifilar linier regression analysis to made model for the period with model like U (850) = a (ISMI) + b (WNPMI) + c (AUSMI) + error.
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Judul: Gene Editing  of Two Cyanide Synthesis-related Genes in Cassava using CRISPR/Cas9
Abstrak: Cassava (Manihot esculenta) contain toxic quantities of cyanogenic glucoside. Reducing the potentially toxic cyanogenic glucoside levels in cassava could be achieved by gene editing through Clustered Regularly Interspaced Short Palindromic Repeats – CRISPR associated protein 9 (CRISPR/Cas9), a high- precision genome editing technique in plants. Two cassava UDP- glucosyltransferase (UGT) paralogs, UGT85K4 and UGT85K5, are the genes catalyzing the synthesis of cyanogenic glucosides in plants. The study aimed to construct the CRISPR/Cas9 recombinant vector carrying single guide RNA (sgRNA) to edit UGT85K4 and UGT85K5 genes, and transform sgRNA CRISPR/Cas9 construct into KU50 cassava calli. Five sgRNAs were designed, considering the on/off target efficiency score and GC content. Those sgRNAs were ligated into pRGEB32 plasmid and introduced to competent Escherichia coli DH5a. The PCR colony and DNA sequencing were performed to verify the sgRNA insert. The amplicons showed the expected band size of recombinant vector (180 bp). Based on sequencing results, only one sgRNA was successfully ligated into the plasmid that will be used to edit the UGT85K5 gene. The sgRNA CRISPR/Cas9 construct in pRGEB32 plasmid was successfully transformed into Agrobacterium tumefaciens and introduced into KU50 cassava calli through co-cultivation. The overgrowth of Agrobacterium occurred after the co-cultivation phase.
Keyword: CRISPR/Cas9, Cyanogenic glucoside, Gene editing, sgRNA