Judul: Elastisitas Permintaan Pangan Strategis berdasar Analisis Data Susenas 2005 dan Implikasinya terhadap Konsumsi dan Upaya Perbaikan Konsumsi Pangan Masyarakat Indonesia
Abstrak: Pemerintah terus berupaya untuk memenuhi permintaan pangan masyarakat, terutama pangan strategis. Permintaan pangan bersifat dinamis, antara lain dapat berubah akibat perubahan harga pangan tersebut, harga pangan lain, dan pendapatan. Seberapa besar pengaruh perubahan tersebut terhadap konsumsi pangan masyarakat merupakan informasi penting sebagai dasar pertimbangan pemerintah dalam menetapkan kebijakan berkaitan dengan perbaikan konsumsi pangan masyarakat Indonesia. Secara umum, penelitian ini bertujuan untuk menganalisis elastisitas permintaan pangan strategis pada rumah tangga di Indonesia. Tujuan khusus penelitian ini adalah menganalisis: 1) konsumsi pangan strategis menurut wilayah dan kelas pendapatan, 2) elastisitas permintaan pangan strategis menurut wilayah dan kelas pendapatan, dan 3) implikasi elastisitas permintaan pangan strategis terhadap konsumsi dan upaya perbaikan konsumsi pangan masyarakat.
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Judul: Analisis konsumsi dan kebutuhan untuk konsumsi pangan di provinsi Sumatera Barat, Jawa Tengah, dan Sulawesi Tenggara tahun 2005-2015
Abstrak: Saat ini Pemerintah Indonesia sedang berupaya keras melaksanakan kegiatan percepatan diversifikasi pangan, dengan harapan pada tahun 2015 konsumsi pangan masyarakat akan mendekati pola pangan harapan (PPH) dengan skor maksimal 100. Oleh karenanya penelitian tentang konsumsi dan permintaan pangan yang dapat memberikan gambaran pencapaian diversifikasi pangan sangat penting untuk dilakukan. Penelitian ini dilakukan dengan menggunakan data Susenas modul konsumsi tahun 2005. Tujuan penelitian adalah: (1) menganalisis tingkat kecukupan energi dan protein serta keragaman konsumsi pangan berdasarkan pendekatan PPH, (2) menganalisis respon permintaan pangan terkait perubahan harga pangan, dan perubahan pendapatan, (3) melakukan estimasi kebutuhan pangan wilayah pada tahun 2008-2015 berbasis data Susenas tahun 2005. Selain Susenas, data lain yang digunakan diantaranya adalah data proyeksi penduduk hasil sensus tahun 2000, dan data pertumbuhan ekonomi. Lokasi penelitian adalah Sumatera Barat, Jawa Tengah, dan Sulawesi Tenggara yang dibedakan antara wilayah perkotaan dan perdesaan.
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Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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