Judul: Sensitivitas Ibu, Kelekatan Ibu-Anak, dan Perkembangan Kognitif Anak Usia Prasekolah di Wilayah Miskin Perkotaan
Abstrak: Penelitian ini bertujuan untuk menganalisis pengaruh sensitivitas ibu, kelekatan ibu-anak, dan perkembangan kognitif anak usia prasekolah di wilayah miskin perkotaan. Penelitian ini dilakukan di Kelurahan Empang dan Tegallega, Kota Bogor yang melibatkan 100 contoh anak yang berusia 3-6 tahun beserta ibu yang dipilih secara proportional random sampling. Hasil penelitian menunjukkan bahwa lama pendidikan ibu dan sensitivitas ibu berhubungan positif signifikan dengan kelekatan ibu-anak. Lama pendidikan ibu dan pendapatan per kapita berhubungan positif signifikan dengan perkembangan kognitif anak. Besar keluarga berhubungan negatif signifikan dengan kelekatan ibu-anak dan perkembangan kognitif. Keikutsertaan PAUD dan lama pendidikan ibu berpengaruh positif terhadap perkembangan kognitif anak. Penelitian ini menunjukkan tidak adanya pengaruh yang signifikan antara kelekatan ibu-anak dengan perkembangan kognitif anak.
Keyword: kelekatan ibu-anak, kemiskinan, PAUD, perkembangan kognitif, sensitivitas ibu

Judul: Sensitivitas Ibu, Keterlibatan Ayah dan Kelekatan Ayah-Anak Usia Prasekolah di Wilayah Perdesaan.
Abstrak: Kelekatan yang terbentuk antara ayah dengan anak usia prasekolah dapat membantu anak untuk dapat mengoptimalkan masa Golden Age dan anak cenderung menjadi pribadi yang santun dan mandiri. Penelitian ini bertujuan untuk menganalisis keterkaitan sensitivitas ibu, keterlibatan ayah dan kelekatan ayah-anak usia prasekolah di wilayah perdesaan. Penelitian ini dilakukan di Kecamatan Ciampea dan Kecamatan Cibungbulang, Kabupaten Bogor dengan melibatkan 50 anak beserta orangtuanya yang ditentukan dengan metode purposive sampling. Hasil penelitian menunjukkan bahwa usia ayah dan lama ayah bekerja berhubungan negatif signifikan dengan kelekatan ayah-anak dan lama pendidikan orangtua, pendapatan per kapita, serta keterlibatan ayah berhubungan positif signifikan dengan kelekatan ayah-anak. Penelitian ini menunjukkan bahwa jenis kelamin anak dan keterlibatan ayah berpengaruh positif signifikan terhadap kelekatan ayah-anak serta usia ayah dan lama ayah bekerja berpengaruh negatif signifikan terhadap kelekatan ayah-anak.
Keyword: anak usia prasekolah, kelekatan ayah-anak, keterlibatan ayah, perdesaan, sensitivitas ibu

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable