Judul: Taraf Pemberian Tepung Daun Bangun-bangun (Coleus ambionicus Lour) dalam Ransum Induk Babi
Menyusui dan Pengaruhnya Terhadap Produksi Air Susu.
Abstrak: Bangun-bangun (Coleus amboinicus Lour) dipercaya masyarakat Batak Toba memiliki khasiat dapat meningkatkan produksi air susu ibu menyusui. Tanaman ini memiliki ciri batang yang lunak, daun bulat dan berbulu, serta memiliki bau yang khas. Penggunaan daun bangun-bangun pada hewan menyusui diharapkan dapat meningkatkan produksi air susu induk sehingga kebutuhan anak akan air susu dapat tercukupi dan pertumbuhan anak menjadi optimal, terutama pada ternak babi yang memiliki litter size lahir yang cukup besar dibandingkan ternak lainnya.
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Judul: Penampilan Anak Babi Menyusu dari Induk dengan Ransum yang Mengandung Tepung Daun Bangun-bangun (Coleus amboinicus Lour) pada Taraf yang Berbeda.
Abstrak: Tanaman bangun-bangun (Coleus amboinicus Lour) merupakan jenis tanaman herba, yang telah lama dikenal di beberapa daerah di Indonesia. Bangunbangun ini dipercaya mampu meningkatkan produksi air susu ibu yang menyusui, terutama di daerah pulau Sumatera Utara. Penelitian ini dilakukan untuk mengetahui dan menentukan pengaruh pemberian beberapa taraf (0; 1,25; 2,5; dan 3,75%) tepung daun bangun-bangun (TDB) ke dalam ransum induk babi menyusui terhadap penampilan anak menyusu hingga penyapihan (30 hari). Peubah yang diamati dalam penelitian ini adalah konsumsi ransum induk, litter size lahir, bobot lahir, produksi air susu induk (PASI) babi, mortalitas, konsumsi ransum anak, litter size sapih, bobot sapih, pertambahan bobot badan anak (PBBA) dan laju pertumbuhan anak babi menyusu.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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