Judul: Pengaruh Dukungan Sosial dan Interaksi Keluarga terhadap Ketahanan Keluarga dengan Istri sebagai Pencari Nafkah Utama
Abstrak: Istri yang bekerja sebagai pencari nafkah utama rentan terhadap kelelahan fisik maupun psikologis yang disebabkan oleh penggunaan waktu dan energi dalam mengerjakan pekerjaan domestik maupun publik. Tujuan umum dari penelitian ini adalah untuk menganalisis pengaruh dukungan sosial dan interaksi keluarga terhadap ketahanan keluarga dengan istri sebagai pencari nafkah utama. Contoh dalam penelitian ini adalah istri yang bekerja sebagai buruh pabrik, memiliki anak usia 0-12 tahun, dan suami tidak memiliki pendapatan tetap. Penelitian melibatkan 30 buruh perempuan yang dipilih secara accidental sampling. Hasil penelitian menunjukkan bahwa dukungan sosial berada pada kategori sedang, interaksi suami-istri berada pada kategori rendah, interaksi orang tua-anak berada pada kategori sedang, dan ketahanan keluarga berada pada kategori sedang. Hasil uji korelasi menunjukkan bahwa terdapat hubungan positif signifikan pendidikan istri dan interaksi keluarga dengan ketahanan keluarga. Faktor yang berpengaruh positif signifikan terhadap ketahanan keluarga adalah pendidikan istri dan interaksi keluarga.
Keyword: dukungan sosial, interaksi keluarga, istri sebagai pencari nafkah utama, ketahanan keluarga

Judul: Pengaruh Dukungan Sosial dan Strategi Koping terhadap Ketahanan Keluarga pada Pernikahan Jarak Jauh
Abstrak: Pengaruh Dukungan Sosial dan Strategi Koping terhadap Ketahanan Keluarga pada Pernikahan Jarak Jauh. Dibimbing oleh TIN HERAWATI dan DEFINA. Pasangan suami istri umumnya tinggal dalam satu rumah. Namun, ada kalanya keluarga tidak tinggal bersama yang disebabkan pasangan tersebut merantau untuk bekerja. Kondisi ini menyebabkan suami istri mengalami kesulitan dalam menjaga ketahanan keluarga. Tujuan penelitian adalah menganalisis pengaruh dukungan sosial dan strategi koping terhadap ketahanan keluarga pada pernikahan jarak jauh. Responden dalam penelitian ini adalah 60 istri yang menjalani pernikahan jarak jauh di eks-Karesidenan Pekalongan. Metode penelitian ini adalah non-probability sampling dengan teknik voluntary sampling. Data dikumpulkan melalui google form dengan kuesioner online. Hasil penelitian menunjukkan bahwa dukungan sosial berada pada kategori rendah, sedangkan strategi koping dan ketahanan keluarga pada kategori sedang. Strategi koping yang sering dilakukan kelurga adalah strategi koping yang berfokus pada emosi. Terdapat hubungan positif signifikan antara strategi koping dengan ketahanan keluarga. Faktor yang berpengaruh positif signifikan terhadap ketahanan keluarga yaitu lama pendidikan suami dan strategi koping., The Influence of Social Support and Coping Strategies on Family Strenght in Long Distance Marriages. Supervised by TIN HERAWATI and DEFINA. Married couples generally live in one house. However, there are times when the family does not live together because the couple migrates to work. This condition causes husband and wife to experience difficulty in maintaining family strenght. The research objective was to analyze the influence of social support and coping strategies on family strenght in long-distance marriages. Respondents in this study were 60 wives who had long-distance marriages in the ex-Residency of Pekalongan. This research method is non-probability sampling with voluntary sampling technique. Data was collected through google form with online questionnaire. The results showed that social support was in the low category, while coping strategies and family strenght were in the medium category. The coping strategy that is often used by the family is a coping strategy that focuses on emotions. There is a significant positive relationship between coping strategies and family strenght. Factors that have a significant positive effect on family strenght are the husband's length of education and coping strategies.
Keyword: dukungan sosial, ketahanan keluarga, pernikahan jarak jauh, strategi koping, coping strategies, family strenght, long distance marriage, social support

Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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