Judul: Kajian Desain Taman Rumah Tinggal Tradisional Budaya Sunda
Abstrak: Sundanese culture is one of the oldest culture in Indonesia. The study of traditional Sundanese garden needs to be done in order to get a real image about traditional Sundanese culture. The objectives of this research are to identify the elements, layout, and interpretation of sundanese garden. This research was obtained through descriptive analysis by field observation and tracing the information of culture and history. The study area are Urug Village, Bogor District; Sindang Barang Village, Bogor District; and Naga Village, Tasikmalaya District. The result of this study showed the spatial order of traditional Sundanese house divided into three parts in according the Tritangtu concept; those are the front or upper part, the central part, and back or under part. Yard of Sundanese house divided into three parts, those are the frontyard (buruan), sideyard (pipir), and the backyard (kebon). The dominant open space is an expanse of land or a grass. The forming elements of traditional sundanese garden are leuit, saung lisung, traditional bathroom (tampian), pond (balong), garden light (obor), cage of hen, and plants.
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Judul: Konsep Taman Rumah Tinggal Tradisional Sunda Masyarakat Adat Rancakalong Sumedang Berbasis Budaya Lokal
Abstrak: Masyarakat adat Rancakalong adalah salah satu masyarakat tradisional di Indonesia yang memiliki cara sendiri untuk memanfaatkan dan melestarikan nilai-nilai adat dan budaya. Hal ini secara tidak langsung dapat mempengaruhi pola lanskap sekitarnya, termasuk taman rumah dan permukiman. Oleh karena itu, pelestarian diperlukan untuk mengantisipasi perubahan budaya. Hal ini dapat diterapkan dengan bentuk implementasi pelestarian kawasan melalui penetapan peraturan daerah, dan kegiatan riset terkait produk budaya. Tujuan dari penelitian ini adalah untuk mengindentifikasi dan menganalisis elemen-elemen taman dan tata letaknya pada taman rumah tinggal Rancakalong dan memformulasikan konsep taman rumah dari sudut pandang budaya masyarakat setempat. Taman rumah tradisional Rancakalong dibagi menjadi tiga bagian, yaitu halaman depan (buruan), halaman samping (pipir), dan halaman belakang (kebon). Elemen hardscape terdiri dari leuit, lisung, kolam (balong), kandang, kayu bakar dan paranggong. Sementara itu, Elemen softscape terdiri dari tanaman yang digunakan dalam sehari-hari dan upacara tradisional. Penempatan elemen taman tidak secara khusus ditentukan oleh aturan adat Rancakalong. Konsep taman rumah tradisional Rancakalong memiliki pola informal yang mencakup elemen hardscape dan softscape yang sesuai dengan budaya dan kebiasaan masyarakat adat Rancakalong
Keyword: lanskap budaya, pelestarian lanskap, desain taman, taman vernakular, permukiman tradisional, Eugenia

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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