Judul: Perencanaan Hutan Kota untuk Meningkatkan Kenyamanan di Kota Gorontalo
Abstrak: Kota Gorontalo terletak di garis lintang nol dengan ketinggian 0 – 500 mdpl, memiliki suhu antara 23,4oC – 32,1oC dan kelembaban antara 78,8 % – 80,5 % apabila ditetapkan indeks kenyamanan maka Kota Gorontalo termasuk dalam kategori kota kurang nyaman. Oleh karena itu, kota yang kurang sampai agak nyaman karena kekurangan luasan daerah yang berpepohonan dapat diubah menjadi kota yang sejuk dan nyaman dengan membangun hutan kota seluas mungkin, yaitu dengan konsep membangun Kota Kebun bernuansa Hutan Kota. Penelitian ini bertujuan untuk: (1) Menganalisis perubahan penutupan lahan dan distribusi suhu serta hubungan luas RTH dengan suhu, (2) Menganalisis kondisi RTH dan hutan kota saat ini meliputi luas, sebaran, bentuk dan fungsi, (3) Menganalisis jumlah kebutuhan RTH dan hutan kota di wilayah Kota Gorontalo berdasarkan luas kawasan, jumlah penduduk dan kenyamanan, (4) Menyusun rencana pembangunan hutan kota di Kota Gorontalo.
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Judul: Evaluation of Thermal Comfort and Ecological Function in GBK Jakarta City Forest
Abstrak: Hutan Kota GBK merupakan salah satu hutan kota yang berada di Kota Jakarta. Hutan Kota GBK berpotensi untuk memperbaiki iklim mikro, menyerap CO2, dan menghasilkan O2. Tujuan penelitian ini adalah mengidentifikasi karakteristik Hutan Kota GBK, menganalisis kenyamanan termal pada Hutan Kota GBK, menganalisis fungsi ekologis pada Hutan Kota GBK, dan menyusun rekomendasi perbaikan pada Hutan Kota GBK. Pada penelitian ini menggunakan metode deskriptif dengan analisis THI, I-Tree Eco, serta persepsi dan preferensi pengunjung untuk mengetahui karakteristik dari Hutan Kota GBK. Pengambilan data dilakukan dengan melakukan pengamatan di lapang, pengukuran data secara langsung, pencarian data sekunder, dan studi literatur. Berdasarkan analisis THI, tingkat kenyamanan termal pada lokasi termasuk dalam kategori tidak nyaman. Pada area rumput dengan naungan memiliki nilai THI yang paling baik. Berdasarkan analisis I-Tree Eco, jumlah karbon tersimpan dan produksi oksigen yang dihasilkan Hutan Kota GBK termasuk kategori rendah. Berdasarkan persepsi dan preferensi pengunjung menunjukkan bahwa Hutan Kota GBK memiliki suhu sejuk, kelembapan udara sedang, pemandangan yang indah, dan fasilitas yang memadai. Namun, diperlukan penambahan fasilitas tempat duduk, WI-FI, dan tempat sampah. Oleh karena itu, diperoleh beberapa rekomendasi yang dapat diimplikasikan untuk meningkatkan kenyamanan dan kualitas lingkungan yang lebih baik di Hutan Kota GBK., GBK City Forest is one of the City Forests in Jakarta. GBK City Forest can potentially improve the microclimate, absorb CO2, and produce O2. This research aims to identify the characteristics of the GBK City Forest, analyze thermal comfort in the GBK City Forest, analyze the ecological function of the GBK City Forest, and develop recommendations for improvements to the GBK City Forest. This research uses a descriptive method with THI analysis, I-Tree Eco, as well as visitor perception and preference to determine the characteristics of the GBK City Forest. Data collection was carried out by conducting field observations, direct data measurements, secondary data searches, and literature studies. Based on THI analysis, the thermal comfort level at the location is included in the uncomfortable category. Grass areas with shade have the best THI values. Based on the I-Tree Eco analysis, the amount of stored carbon and oxygen production produced by the GBK City Forest is in the low category. Based on visitor perceptions and preferences, it shows that GBK City Forest has cool temperatures, moderate air humidity, beautiful views, and adequate facilities. However, additional seating facilities, WI-FI, and trash cans are needed. Therefore, several recommendations have been obtained which can have implications for increasing comfort and better environmental quality in the GBK City Forest.
Keyword: fungsi ekologis, hutan kota, I-Tree Eco, kenyamanan termal, THI

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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