Judul: Pengaruh Ph Terhadap IgG Anti H5N1 Asal Kolostrum Sapi Yang Divaksin Dengan Vaksin Avian Influenza H5N1
Abstrak: The aims of this study is to determine the stability of IgG anti-H5 from bovine colostrum (BC) after exposure to acid and basic pH. Two samples of BC, whole colostrum and whey colostrum incubated at pH 4, 5, 9, and 10 for 30 and 60 minutes. Haemagglutination Inhibition (HI) test is used to determine IgG titer after incubation. IgG titers decreased after exposure to acid or basic pH. Acid exposure (pH 5) decreased antibodi titer of whole colostrum as much as 3,75% and 16,25% after incubation 30 and 60 minutes respectively. Antibody titer to pH 4 also decreased until 12,5% and 16,5% after for 30 and 60 minutes respectively. In whey colostrum, both pH 4 and pH 5 reduce 18,75% antibody titer. On other hand, IgG titers is decreased until 100% at basic pH (pH 9-10). In conclusion, IgG from BC is degraded in acidic and alkaline conditions.
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Judul: Karakterisasi Protein IgG Anti H5N1 Menggunakan Metode SDS-Page (Sodium Dodecyl Sulfate Polyacrilamide Gel Electrophoresis) Dari Kolostrum Sapi Yang Divaksin H5N1
Abstrak: This experiment was conducted to characterize immunoglobulin gamma (IgG) in colostrum of Friesian Holstein (FH) cow which vaccined by Avian Influenza virus vaccine subtype H5N1. Samples was divided into three sample, Kol I Sp4, Kol II Sp4, Kol III Sp4. These samples were compared to IgG control. IgG control was colostrum sample from unvaccinated FH cow. Characterization of IgG were done by SDS-PAGE method. The result showed that IgG control have three band proteins and the IgG colostrums from vaccinated cow have five to six band proteins. The molecular weight (MW) of these protein samples ranged between 19.49 to 228.09 kDa. There were differences between the molecular weight of sample protein Kol I SP4, Kol II SP4, and Kol III SP4 with IgG control. Based on the protein size bands, there were different between Kol I SP4, Kol II SP4, and Kol III SP4. There were some unidentified protein in the sample. Conclusion, MW of IgG from colostrum samples vaccinated are 147.37 kDa, 147.37 kDa, 140.57 kDa and the IgG control was 161.57 kDa.
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Judul: Corsa.id: Penyedia Jasa Konstruksi Berbasis Marketplace
Abstrak: Pengguna jasa konstruksi kesulitan dalam mencari dan menemukan penyedia jasa konstruksi. Oleh karena itu peneliti menawarkan sebuah alternatif solusi berupa aplikasi penyedia jasa konstruksi. Tujuan dari penelitian ini adalah untuk mengidentifikasi masalah yang dihadapi pengguna dan penyedia jasa konstruksi, merancang prototype aplikasi Corsa.id, dan menyusun model bisnis Corsa.id. Penentuan responden menggunakan teknik non probability sampling dengan pendekatan purposive sampling. Responden penelitian ini dibagi menjadi dua kategori yaitu pengguna dan penyedia. Metode yang digunakan adalah customer discovery dan lean canvas. Hasil dari penelitian ini menunjukkan bahwa responden pengguna jasa konstruksi merasakan beberapa kesulitan dalam mencari penyedia jasa konstruksi dan responden penyedia jasa konstruksi juga merasakan kesulitan dalam mencari client. Sebagai solusi model bisnis dan prototype aplikasi Corsa.id dibentuk. Hasil dari pengujian solusi menunjukkan bahwa prototype Corsa.id dapat menjadi solusi. Verifikasi model bisnis juga menunjukkan model bisnis Corsa.id sudah sesuai dan terverifikasi.
Keyword: aplikasi, customer discovery, lean canvas, pengguna jasa konstruksi, penyedia jasa konstruksi