Judul: Comparative Analysis of Food Consumption Patterns in Urban and Rural Adolescents in the Bogor Region
Abstrak: Remaja di perkotaan dan perdesaan diduga memiliki perbedaan praktik konsumsi pangan akibat perbedaan gaya hidup dan faktor-faktor yang memengaruhinya. Kondisi ini bisa membawa pada perbedaan pola konsumsi pangan dan asupan gizi remaja perdesaan dan perkotaan. Penelitian ini bertujuan membandingkan pola konsumsi pangan, gaya hidup, dan akses dalam mendapatkan pangan pada remaja di perkotaan dan perdesaan. Desain penelitian yang digunakan adalah cross sectional. Hasil penelitian menunjukkan bahwa pola konsumsi remaja perkotaan dan perdesaan memiliki beberapa persamaan frekuensi jenis pangan yang dikonsumsi. Gaya hidup berdasarkan variabel frekuensi makan utama, kebiasaan sarapan, frekuensi membawa bekal, dan konsumsi makanan melalui media berbeda signifikan (p<0,05). Variabel susunan menu makan, cara pengolahan makanan, kebiasaan jajan, sumber media informasi fast food, dan bagian iklan tidak berbeda signifikan (p>0,05). Akses sarana dan keterjangkauan pangan berdasarkan variabel asal perolehan bahan pangan pokok mentah dan matang, serta jenis transportasi untuk membeli makanan berbeda signifikan (p<0,05). Variabel ketersediaan makanan dan pengaruh paket promosi fast food tidak berbeda signifikan (p>0,05).
Keyword: adolescents, consumption patterns, lifestyle, rural, urban

Judul: Hubungan antara Kebiasaan Makan dengan Status Gizi pada Remaja di Perkotaan dan Perdesaan
Abstrak: Double burden of nutritional problems are not only faced by urban societies but also rural societies. This study aimed to analyze association of eating habits, physical activities and nutritional status of adolescence in urban and rural areas. The study used cross sectional design involved 102 adolescences. There were significant differences (p<0.05) between subject in urban and rural in term of age, daily pocket money, parents’ educational background, family income, family size, frequency consumption of noodles, chicken, tofu, soybean, carrot, banana, orange, physical activity of school day. There was no significant difference in frequency of a having meal and habit on buying snacks. There was significant correlation between ages, genders, parents’ educational level, family size, family income and nutritional status (TB/U), as well as gender and nutritional status (IMT/U). However, there was no significant correlation between eating habits, physical activities, energy and nutrients adequacy levels and nutritional status (p>0.05).
Keyword: nutritional status, adolescence, food consumption, eating habits, physical activities

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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