Judul: Evaluasi Tata Hijau Jaian Bebas Hambatan Prof. Dr. Ir. Sedyatmo Jakarta.
Abstrak: Jalan bebas hambatan Prof. Dr. Ir. Sedyatmo men~pakan akses tercepat dari ibukota Jakarta untuk menuju Bandara Internasional Soekamo Hatta dengan tingkat kecepatan relatif tinggi karena tingkat urgensi pengguna jalan bebas hambatan tersebut untuk segera tiba di bandara. Lokasi jalan bebas hambatan Prof. Dr. Ir. Sedyatmo yang mernbelah hutan b'akau menimb~llkan persoalan tersendiri dimana kekuatan tanah yang kurang stabil imtuk menopang tingkat lalu lintas yang tinggi. Tujuan dari studi ini adalah mengevaluasi fungsi dan estetika tata hijau jalan bebas hambatan ~ro'f. Dr. Ir. Sedyatmo dengan menggunakan standar i l m ~y~an g terdapat pada ilmu arsitektur lanskap. Hasil evaluasi ini berguna untuk rnengetahui permasalahan dan mencari alternatif pemecahannya. Aspek fungsi yang dievaluasi adalah fungsi tanaman sebagai kontrol visual, pengarah, kontrol kesilauan, peredam kecelakaan, pemberi identitas, mengurangi kebisingan, mengurangi polusi, konservasi dan penilaian terhadap estetika dari pemilihan dan penataan tanaman yang meliputi jenis tanaman, disain tanaman, kualitas visual dengan memperhatikan warna. ukuran, tekstur, bentuk, tema, gradasi, kontras dan keseimbangan.
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Judul: Evaluasi tata hijau jalur hijau jalan Kota Pekanbaru
Abstrak: Jalur hijau kota Pekanbaru didominasi penanaman pohon dengan berbagai macam bentuk tajuk dan juga penanaman perdu dan penutup tanah dengan berbagai macam pola. Evaluasi terhadap tata hijau pada lanskap jalan dilakukan untuk meningkatkan dan mengembangkan tata hijau pada lanskap jalan. Penelitian bertujuan untuk mengevaluasi aspek fungsi, estetika dan pengelolaan jalur hijau dan memberikan rekomendasi peningkatan fungsi, estetika dan pengelolaan tata hijau jalur hijau jalan. Penelitian ini bermanfaat memberikan masukan kepada perencana, perancang dan penegelola dalam mengembangkan jalur hijau jalan kota Pekanbaru.
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Judul: DNA Extraction and Characterization of Salmonella‟s Lytic Bacteriophage Genome
Abstrak: Bacteriophages are ubiquitous in nature and are known to proliferate wherever their bacterial hosts exist. Their virion particles can exist independently outside the host, however all phages are need their host to propagate. Several phages are highly specific to host cell surface receptors and any slight changes in structure results in little or no interaction between the phage and its host. Salmonella‟s phage isolated from sewage water where their bacteria exist. Three isolated of phages (phages 15, 19 and 38) were used in this research that concentrated using three types of methods. PEG/ NaCl method was firstly used to concentrate the phages based on their molecular weight to precipitate the phage particles during centrifugation. The second method was Scraping Plaque method. In this method, overlay plaques were scraped and collected in SM buffer to get phage concentrates. Enrichment method was the last method used to concentrate the phages. Phage genome isolation was done using two methods i.e. EDTA/SDS and Proteinase K methods. The first method was done using DNase I to eliminate bacterial DNA followed by SDS/EDTA treatment to release phages DNA and phenol-chloroform-isoamyl alcohol to extract the DNA. DNA was collected by isopropanol extraction. While in Proteinase K method, bacterial DNA was eliminated by DNase I and Proteinase K/SDS was used to degrade phages capsids and ethanol for DNA precipitation. The result showed that PEG/NaCl method resulted the highest phage concentration for phage 15 and phage 19, while scrapping method was the highest for phage 38. The quantity and purity of extracted DNA were low for PEG/NaCl and Scraping methods, while Enrichment method was higher indicated higher purity. Run on agarose gel electrophoresis of extracted DNA confirmed that Enrichment method results were the best while scraping and PEG/NaCl method was appeared smear background. Based on DNA genome size determination, the bacteriphages of Salmonella sp were comparable with other study of Salmonella phages. Phage P15 with genome size of 39,5 kb was close to phage epsilon15. Phage P19 with genome size of 41 kb was close to phage ST160. And phage P38 with genome size of 48,5 kb was close to phage Gifsy-1.
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