Judul: Etnografi, Etnobotani dan Etnoekologi Masyarakat Etnik Batak Toba di Kecamatan Simanindo Kabupaten Samosir
Abstrak: Hasil penelitian didapatkan bahwa kearifan lokal masyarakat Etnik Batak Toba Kecamatan Simanindo adalah kearifan lokal terkait ritual keagamaan dengan memanfaatkan tumbuhan. Pemanfaatan tumbuhan dalam ritual keagamaan seperti prosesi pemakaman, tasyakuran hasil bumi, tahun baru agama, dan mengkramatkan suatu spesies tumbuhan. Tumbuhan tersebut adalah Ficus benjamina, Themeda gigantea, Cordyline fruticosa, Oryza sativa, Crinum jagus, Ocimum americanum. Tumbuhan tersebut dijaga keberadaannya dengan cara membudidayakannya di sekitar ladang, sawah dan pekarangan rumah. Selain hal tersebut juga ada pemanfaatan tumbuhan dalam tradisi menyirih/menginang seperti tumbuhan sirih (Piper bettle), tembakau (Nicotiana tabacum), gambir (Uncaria gambir), pinang (Areca cathecu) dan penggunaan tumbuhan sebagai pewarna ulos, seperti, kayu jabi-jabi (Sida acuta), daun salaon (Indigofera tinctoria), kayu sona (Pterocarpus indicus), daun tayom, kunyit (Curcuma domestica) dan harumonting (Rhodomyrtus tomentosa).
Keyword: Cosmos, Corpus, Etnobotani, Etnoekologi, Etnografi

Judul: Ethnobiology of thesamin
Abstrak: This ethnobiological research focus on the ethnoecological, ethnobotanical and ethnozoological study of the adaptation process (correlating to management concept, impact on people’s activities, and technology usage) of Samin Community. The goals of this research were to study the beliefs, knowledge, and practice the Samin community for the comprehensive understanding of landscape use and management, ant to reveal the local knowledge of Samin community in managing their natural resource (plants and animals) whice include species diversity, the index of ecological importance value (INP), and the index of cultural significance (ICS). The study was conducted during the period of August 2009 to December 2011. The research was carried out in 7 villages in 4 districts, namely: (1) Larikrejo and (2) Kaliyoso (Kudus District); (3) Bombong and (4) Ngawen (Pati District); (5) Klopoduwur and (6) Tambak, Sumber (Blora District); (7) Jepang, Margomulyo (Bojonegoro, East Java). The research data consisted of ecological, ethnobotanical, ethnozoological and ethological data. Data collection using direct observation; open ended and structured interview, and participant observation. The results showed that the Samin society has a fairly good knowledge of biological resources and their environment. Use practices and management of natural resources is reflected in the form units of the environmentals and biological resources contained therein. Knowledge of the spatial environment simply divided into two main units, namely: mondokan (house and yard), and lemah garapan includes: fields (sawah), moor (tegalan) and teak (alas jati). Paddy field, dry field, yard, garden, teak forests, marsh ponds, and rivers were the places where the cultivation of a wide variety of production activities of plants and animals which were conducted to meet their basic needs. The research obtained more than 300 of plant species in the Samin settlement, which 235 species among those were useful for their life. Utilization of those plants include for food (118 species), traditional medicine and cosmetics (74 species), building materials (17 species) equipment (15 species), firewood (16 species), animal feed (27 species), fiber materials and the straps (3 species), ritual materials (26 species), toxic materials (2 species), pest control materials (16 species), environmental indicators (5 species), and ornamental plants and fences (45 species). Based on the role and utilization of animals, it can be grouped into: animal protein source (21 species), pets (7 species), pests of cultivated plants (17 species), pests of livestock (3 species), pest predators (11 species), animals for medicine (10 species), animals for ritual (1 species), and wildlife (35 species). The Samin community the resources and the environment only as necessery. They always maintained a harmonious relationship with nature.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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