Judul: Pengaruh Pelaksanaan Keputusan Presiden RI No. 32 Tahun 1990 terhadap Produksi Kayu Bulat di Wilayah Kerja Perum Perhutani Propinsi Dati I Jawa Timur
Abstrak: Hutan mempunyai banyak manfaat antara lain produksi kayu dan perlindungan, kedua manfaat tersebut sama pentingnya sehingga sulit menentukan prioritas manfaat yang kadang-kadang bertentangan. Agar hutan dapat dimanfaatkan semaksimal mungkin diperlukan Undang-Undang atau peraturan-peraturan di bidang kehutanan yang harus dilaksanakan.
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Judul: Pengaruh Perebusan Terhadap Kadar Pati Kayu Pulai (Alstonia R.Br.),Kemiri (Aleurites moluccana (I,) Willd.), dan Nyatoh Kuning (Planchonella nitida Dub.)
Abstrak: Indonesia mempunyai sumberdaya hutan seluas 143 juta hektar dengan jenis pohon yang beraneka ragam (* 4000 jenis kayu). Nanlun demikian selama psriode 1981 - 1990 kemsakan hutan alam di Indonesia mencapai 1,3 juta hektar per tahun seiring dengan itu target produksi kayu bulat Indonesia dari areal Hak Pengusahaan Hutan (HPH) menurun dari 37 juta m3 per tahun awal pelita V menjadi 22 juta m3 per tahun mulai awal pelita VI (Departemen Kehutanan, 1995 dalam Kartodihardjo, 1999) padahal kebutuhan kayu ten~s meningkat. Salah satu altematif untuk mengatasi masalah tersebut adalah pelnailfaatan kayu dari hutan rakyat.
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Judul: DNA Extraction and Characterization of Salmonella‟s Lytic Bacteriophage Genome
Abstrak: Bacteriophages are ubiquitous in nature and are known to proliferate wherever their bacterial hosts exist. Their virion particles can exist independently outside the host, however all phages are need their host to propagate. Several phages are highly specific to host cell surface receptors and any slight changes in structure results in little or no interaction between the phage and its host. Salmonella‟s phage isolated from sewage water where their bacteria exist. Three isolated of phages (phages 15, 19 and 38) were used in this research that concentrated using three types of methods. PEG/ NaCl method was firstly used to concentrate the phages based on their molecular weight to precipitate the phage particles during centrifugation. The second method was Scraping Plaque method. In this method, overlay plaques were scraped and collected in SM buffer to get phage concentrates. Enrichment method was the last method used to concentrate the phages. Phage genome isolation was done using two methods i.e. EDTA/SDS and Proteinase K methods. The first method was done using DNase I to eliminate bacterial DNA followed by SDS/EDTA treatment to release phages DNA and phenol-chloroform-isoamyl alcohol to extract the DNA. DNA was collected by isopropanol extraction. While in Proteinase K method, bacterial DNA was eliminated by DNase I and Proteinase K/SDS was used to degrade phages capsids and ethanol for DNA precipitation. The result showed that PEG/NaCl method resulted the highest phage concentration for phage 15 and phage 19, while scrapping method was the highest for phage 38. The quantity and purity of extracted DNA were low for PEG/NaCl and Scraping methods, while Enrichment method was higher indicated higher purity. Run on agarose gel electrophoresis of extracted DNA confirmed that Enrichment method results were the best while scraping and PEG/NaCl method was appeared smear background. Based on DNA genome size determination, the bacteriphages of Salmonella sp were comparable with other study of Salmonella phages. Phage P15 with genome size of 39,5 kb was close to phage epsilon15. Phage P19 with genome size of 41 kb was close to phage ST160. And phage P38 with genome size of 48,5 kb was close to phage Gifsy-1.
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