Judul: Uji Toksisitas Recombinant Growth Hormone terhadap Artemia salina Leach.
Abstrak: Hormon pertumbuhan rekombinan (rGH) merupakan protein yang diproduksi dengan memanfaatkan bakteri Escherichia coli yang membawa vektor ekspresi gen hormon pertumbuhan dan terbukti mampu meningkatkan laju pertumbuhan ikan maupun udang. Potensi toksisitas rGH ikan kerapu kertang (rElGH) belum diketahui. Penelitian ini bertujuan untuk menentukan tingkat dan golongan toksisitas rGH ikan kerapu kertang terhadap A. salina menggunakan metode brine shrimp lethality test (BSLT). Konsentrasi rElGH yang digunakan, yaitu: A. 1,5; B. 15; C. 150; dan D. 1,500 mg L-1 dengan lima kali ulangan. Sebagai kontrol, digunakan dua perlakuan, yakni perlakuan K1 hanya menggunakan 20 mL air laut, dan perlakuan K2 dengan pemberian 3 mL PBS (pelarut rElGH) dan 17 mL air laut. Kepadatan A. salina yang digunakan yakni 100 ekor dalam 20 mL larutan yang berisi 3 mL rElGH dan 17 mL air laut. Pengamatan terhadap jumlah naupli yang mati dilakukan setiap 6 jam sekali selama 24 jam pengamatan. Persentase kematian pada perlakuan A, B, C, dan D berturut-turut ialah 4,0%; 11,2%; 26,6%; dan 100%. Berdasarkan hasil analisis probit Minitab 16, nilai LC50 adalah 222,05 mg L-1, sehingga rElGH digolongkan dalam senyawa dengan tingkat toksisitas rendah.
Keyword: A. salina, BSLT, rElGH, toksisitas

Judul: Respons benih ikan sidat terhadap hormon pertumbuhan rekombinan ikan kerapu kertang melalui perendaman dan oral
Abstrak: This study was aimed to determine an appropriate administration method and observe the response of eel fingerling on recombinant giant grouper growth hormone (rElGH). First study aimed to determine the immersion dose of rElGH to increase growth of eel juvenile (glass eel). After shock salinity treatment (NaCl 3% for 2 min), glass eel were immersed in water containing 0.9% NaCl, 0.01% bovine serum albumin, and different of dose of rElGH (0, 0.12, 1.2, 12 and 120 mg/L). The results showed that higher in growth body weight was obtained in treatment 12 mg/L (101.2±0.8), by increment of about 37.4% higher compared to that of control (72.93±1.8). Second study was aimed to determine the dose of rElGH mixed in artificial diet to increase growth of eel elver. HP55-coated rElGH was mixed to diet at different doses (0, 0.3, 3, and 30 mg/kg). Elver was fed on diet containing rElGH twice daily, 6% fish biomass. The result showed that higher in growth body weight was obtained in treatment 30 mg/kg (60.18±1.38), by increment of about 65.7% compared to control (36.32±0.97). Third study was aimed to compared of three administration method, namely immersion, oral, and combination of immersion and oral. The dosage of rElGH by immersion (12 mg/L) and oral administration (30 mg/kg feed) was obtained in previous study. Fish were maintained in 23-L glass aquaria for 120 days, at density of 45 fish per aquarium. The results showed that higher (P<0.05) biomass of harvest (73.68±2.07 g) was obtained in immersion and oral combination treatment, by increment of about 102.9% compared to control (36.32±0.97 g). Administration of rElGH also improved protein and lipid retentions by 142.5%, and 720% compared to that of control, respectively (P<0.05). In addition, rElGH treatment increased appetite, while artificial feed conversion ratio (4.75) was lower (P<0.05) by 55.15% compared to that of control (7.37). Combination of rElGH immersion and oral administrations also increased hepatosomatic index, reduced ammonia excretion and increased insuline-like growth factor-1/IGF-1 gene expression. Thus, rElGH administration via immersion and oral was a proper method to improve performance of eel fingerling, and application of rElGH can be useful to increase aquaculture production.
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Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4