Judul: Peningkatan Umur Simpan Tempe Bacem dengan Metode Vakum pada Beberapa Kondisi Penyimpanan
Abstrak: Tempe bacem is a kind of tempe product that has spicy and sweet taste. The shelf life of this product is very short. The objective of this research was to increase tempe bacem’s shelf life by vacuum and non-vacuum packaging that stored in different conditions. Tempe that used in this research was in round form with 5 cm of diameters and 1 cm of thick. Tempe bacem was processed by selected formula, packaged by vacuum and non-vacuum and then stored in room condition (26-300C) and refrigerator condition (100C). Total protein in this product was 33.7% (db), fat 0.9% (db), ash 3.0% (db), carbohydrate 66% (db), while antioxidant capacity was 194.65 mgAEAC/100 g tempe. The pH value in tempe bacem was 5.7, lightness 35.9, hardness 2848.5 gram force, total TPC 1.8x103 CFU/g and total koliform <3.0 MPN/g. The shelf life of tempe bacem that packaged by non-vacuum method in room condition was 2 days, and in refrigerator condition was 6 days. The shelf life of tempe bacem that packaged by vacuum method in room condition was 3 days and in refrigerator condition was 18 days.
Keyword: Bogor Agricultural University (IPB), tempe bacem, shelf life, vacuum packaging

Judul: Mempelajari Keawetan Tempe Pasteurisasi Dalam Kemasan Vakum HDPE dan Alumunium Foil
Abstrak: Tempe is very perishable food at room temperature. Several techniques used to process tempe for its preservation have been developed. These processing techniques include drying, frying, and canning have been used to achieve long shelf life of tempe, with the significant loss of tempe’s freshness. The objective of this research was to evaluate the effect of pasteurization process, as a milder heat treatment, in combination with vacuum packaging on extending shelf life of tempe. Using a target microbe of nonproteolytic C. botulinum with z value of 7.2 oC and D85 of 0.28 minutes, the extent of pasteurization process was based on the heat penetration data and calculated as Pv = P85 (z=7.2). Our result indicated that 6D reduction of C. botulinum was easily achieved at all temperatures of pasteurization. Futhermore, from several combinations of time and temperature studied, the optimum pasteurization value resulted in maximum shelf life of pasteurized tempe. The optimum pasteurization values of tempe vacuum packed in HDPE and stored at 5, 15, and 20 oC were 35.05, 35.36 and 33.30 minutes. The associated maximum shelf life of the tempe were 24, 16, and 13 days. For tempe vacuum packed in aluminium foil and stored at 5, 15, and 20 oC, the optimum pasteurization values were 37.04, 35.44, and. 42.47 minutes. These optimum pasteurization values resulted in shelf life of 25, 17, and 12 days. Therefore, the longest shelf life of 25 days was obtained from P85 (z=7.2) of 37 minutes by using aluminium foil packaging and stored at 5 oC. The anomaly observations, in which the shelf life was not proportional to the pasteurization value, was interesting and thought to be associated with complex microbiological nature of tempe as fermented soyfood.
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Judul: Posisi Filogenetik Kura-Kura Carettochelys Insculpta  (Reptillia: Testudinata)
Abstrak: Carettochelys insculpta is an endemic freshwater turtle of southern Papua and northern Australia. Phylogenetic position of this turtle is remain unresolved until now. Most of the research conducted show close relationships of C. insculpta and members of Trionychidae family. However, some of the research show distance between C. insculpta and members of Trionychidae family. This research is conducted to analyze phylogenetic relationship between C. insculpta and other members of Testudinata using 12S rRNA, 16S rRNA, and both gene as markers. DNA samples were obtained from three different organisms of C. insculpta. Two species used as outgroups were Alligator mississippiensis and Iguana iguana. Phylogenetic analysis was carried out using Neighbor-Joining method with Felsenstein 1981 (F81) modeltest. A polytomy tree of Testudinata was produced by using 12S rRNA gene. However, analysis using 16S rRNA and both genes as markers confirmed that C. insculpta was located between the Chelonidae and the Trionychidae family.
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