Judul: Studi Morfologi dan Molekuler Boesenbergia angustifolia (Hallier f.) Schltr. dan Boesenbergia sp.
Abstrak: Zingiberaceae is the largest family of Zingiberales with 53 genera, over 1200 species and spread in the tropics. Boesenbergia is the one of Zingiberaceae genera, that has not been explored, and therefore, in this research morphological and phylogenetic studies were conducted using Boesenbergia angustifolia and Boesenbergia sp. of Sumatra. The method used in this study is the observation of morphological characters and molecular characters in based of variation in Internal Transcribed Spacer (ITS) sequence are used for phylogenetic tree construction. DNA sample was isolated using cetyltrimethylammonium bromide based method of Doyle & Doyle modification. Amplification of ITS region resulted in ± 800 bp amplicon. Sequencing DNA of ITS region resulted in ± 788 bp amplicon from Boesenbergia angustifolia and ± 789 bp amplicon from Boesenbergia sp. Recontruction of phylogenetic tree was analysed using the Phylogenetic Analysis Using Parsimony program. The morphological characters of Boesenbergia angustifolia and Boesenbergia sp have similar flowers and inflorescence character. Suggestion that they are of the same genera. This is also supported by ITS DNA sequence, both of these plant species have 17 bases difference. Phylogeneticly, these plant species also form branching close to the high level of confidence with 97% bootstrap value. They are of the same veriety.
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Judul: Studi Molekuler untuk Menentukan Kekerabatan Genus Zingiber Varietas Zerumbet
Abstrak: Problems due to opinion differences in medicinal plants classification cause confusion on the use of scientific name of plants. The grouping of plants which based on morphology in some cases is less effective and it is necessary to perform molecular studies used maturase (matK) and Internal Transcribe Spacer (ITS) as molecular markers. The study was aimed to classify members of the Zingiber genus Zerumbet subsection that is Zingiber zerumbet, Zingiber aromaticum, and Zingiber amaricans. Stages of research were isolation of DNA, DNA amplification, DNA sequencing, alignment of DNA sequences, and construction of phylogenetic tree. Results showed that the isolated DNA was of good quality. The size of the amplified DNA with the matK was 2000–3000 bp, while the size of the amplified DNA with the ITS was 900 bp. ITS sequences were of low quality, so that only the matK sequences were used for inference. Based on the results of sequence alignment with data obtained from GenBank differences between members of the genus Zingiber subsection Zerumbet with other types of Zingiber was 1–11 bp and the phylogenetic tree with consistency index (CI)= 0.897 and retention index (RI)= 0.833 shows a very close genetic relationship between the three members of the Zingiber genus Zerumbet subsection with bootstrap (BS)= 59. The third member of the Zingiber genus Zerumbet subsection is spesies kompleks which is under the type of Zingiber zerumbet.
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Judul: Peranan Public Relations Pada Perusahaan Media Massa Televisi Setelah Relaunch Perusahaan (Kasus Marketing Public Relations Perusahaan Media Massa Televisi Trans7)
Abstrak: Strategi yang ditempuh perusahaan untuk menaikkan citranya adalah dengan melakukan hubungan dengan publik atau kegiatan Public Relations. Peranan Public Relations tidak hanya ke dalam perusahaan atau organisasi saja tetapi juga keluar perusahaan. Kegiatan Public Relations berperan dalam menyusun strategi komunikasi yang tepat untuk menaikkan citra perusahaannya. Dalam hubungannya dengan pencitraan positif terhadap publik internal, Public Relations berperan untuk menumbuhkembangkan tanggung jawab sosial perusahaan. Dengan demikian, akan menciptakan motivasi kerja, loyalitas, dan peningkatan kinerja karyawan. Penelitian ini bertujuan untuk : 1). Mengkaji proses perubahan manajemen perusahaan dari TV7 menjadi Trans7, dan 2). Mengkaji peran PR dalam pembentukan citra perusahaan dan mengkaji strategi yang dilakukan perusahaan dalam mempromosikan program yang ditayangkan setelah perusahaan melakukan relaunch. Metode penelitian ini menggunakan pendekatan kualitatif. Penerapan metode kualitatif ini menggunakan strategi penelitian kasus. Trans7 terletak di Menara Bank Mega, Jalan Kapten P. Tendean Kav. 12- 14A Lantai 20, Jakarta Selatan. Berawal dari kerjasama strategis antara Para Group dan Kelompok Kompas Gramedia pada tanggal 4 Agustus 2006, Trans7 lahir sebagai sebuah stasiun swasta yang menyajikan tayangan yang mengutamakan kecerdasan, ketajaman, kehangatan penuh hiburan serta kepribadian bangsa yang membumi. Trans7 yang semula bernama TV7 berdiri dengan ijin dari Departemen Perdagangan dan Perindustrian Jakarta Pusat dengan Nomor 809/BH.09.0S/III/2000. Pada tanggal 22 Maret 2000 keberadaan TV7 telah diumumkan dalam Berita Negara Nomor 8687 sebagai PT. Duta Visual Nusantara Tivi Tujuh.
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