Judul: Viability of Probiotics Fermentation Maggot (Hermetia illucens) on Temperature and Storage Time
Abstrak: Maggot (Hermetia illucens) merupakan insekta yang berpotensi dijadikan sebagai probiotik karena memiliki kandungan antimicrobial peptide (AMP) yang mampu menghambat pertumbuhan bakteri patogen pada saluran pencernaan inang. Masa simpan merupakan hal yang harus diperhatikan supaya menjaga kualitas suatu produk. Penelitian ini bertujuan menganalisis viabilitas probiotik fermentasi maggot yang diberi pengaruh lingkungan khususnya suhu dan lama waktu penyimpanan. Penelitian ini menggunakan 48 sampel probiotik fermentasi maggot, terdiri dari 12 perlakuan dan 4 ulangan yang dianalisis menggunakan Rancangan Acak Lengkap (RAL) pola faktorial yang terdiri dari 2 faktor. Faktor I merupakan perlakuan lama waktu penyimpanan dengan taraf B1: bulan ke-1, B2: bulan ke-2, dan B3: bulan ke-3. Faktor II merupakan suhu penyimpanan dengan taraf yaitu S1: 4°C, S2: 28°C, S3: 38°C, dan S4: 48°C. Parameter yang diamati yaitu kualitas fisik, populasi mikroba, total asam tertitrasi (TAT), dan zona hambat. Hasil penelitian menunjukkan adanya penurunan pada viabilitas probiotik seiring dengan lamanya penyimpanan. Masa simpan bepengaruh pada nilai total asam dan populasi bakteri asam laktat tapi tidak pada populasi mikroba lainnya dan zona hambat., Maggot (Hermetia illucens) is an insect that has potential to be used as a probiotic because it contains antimicrobial peptide (AMP) which can inhibit the growth of pathogenic bacteria in the digestive tract of the host. Shelf life is something that must be considered to maintain the quality of a product. This study aimed to analyze the viability of maggot probiotics which are influenced by the environment, especially temperature and storage time. This study used 48 maggot probiotic samples, consisting of 12 treatments and 4 replications which were analyzed using a completely randomized design (CRD) factorial pattern consisting of 2 factors. First factor was long storage time treatment with levels B1: 1st month, B2: 2nd month, and B3: 3rd month. Second factor is storage temperature with levels S1: 4°C, S2: 28°C, S3: 38°C, and S4: 48°C. The parameters observed were physical quality, population of microbes, titratable acidity (TA), and antimicrobial activity of maggot probiotics. The results showed that there was a decrease in the viability of probiotics along with the length of storage. Shelf life has an effect on the total acid and lactic acid bacteria, but has no effect on the population of other microbes and antimicrobial activity.
Keyword: maggot, probiotics, storage time, temperature, viability

Judul: Effect of E. faecalis probiotics supplementation from Maggot H. illucens on In Vitro Digestibility and rumen Fermentability
Abstrak: Tujuan penelitian ini adalah untuk menguji pengaruh suplementasi probiotik E. faecalis yang berasal dari maggot H. illucens dengan level yang berbeda secara in vitro berdasarkan kecernaan dan fermentabilitasnya dalam rumen. Penelitian ini menggunakan rancangan acak kelompok dengan 3 perlakuan dan 4 ulangan. Perlakuan terdiri dari P0 (tanpa suplementasi probiotik), P1 (suplementasi probiotik 0,5%), dan P2 (suplementasi probiotik 1%). Parameter yang diukur meliputi nilai pH, Ammonia (NH3), VFA(volatile fatty acid) total, VFA parsial, produksi gas metan (CH4), populasi bakteri, populasi protozoa, koefisien cerna bahan kering (KcBK) dan koefisien cerna bahan organik (KcBO). Hasil menunjukkan bahwa setiap perlakuan tidak berpengaruh nyata terhadap nilai pH, Ammonia (NH3), VFA parsial, CH4, populasi bakteri, populasi protozoa, KcBK dan KcBO, namun suplementasi probiotik E. faecalis 0,5% berpengaruh nyata dalam menurunkan nilai VFA total. Berdasarkan hasil penelitian dapat disimpulkan bahwa suplementasi probiotik E. faecalis asal maggot H. illucens pada level 0,5% dan 1 % belum dapat bekerja secara optimal terhadap fermentabilitas dan kecernaan rumen. Suplementasi probiotik E. faecalis pada level 0,5% dan 1% tidak mengganggu aktivitas fermentasi di dalam rumen., The study aimed to examine the effect of supplementation of E. faecalis probiotics from maggot H. illucens with different levels on in vitro digestibility and rumen fermentability. This study used a randomized block design (RBD) with 3 treatments and 4 replicates. The treatments were P0 (diet without probiotic), P1 (diet supplemented with 0.5% probiotics), and P2 (diet supplemented with 1% probiotics). Parameters on this research are pH value, NH3 (ammonia), total VFA (volatile fatty acid), partial VFA, methane gas production (CH4), rumen protozoa population, rumen bacterial population, dry matter digestibility (DMD), and organic matter digestibility (OMD). The results showed that each treatment have no significant effect on pH value, NH3 (ammonia), partial VFA, CH4, rumen protozoa population, rumen bacterial population, dry matter digestibility (DMD), and organic matter digestibility (OMD), but supplementation of 0.5% probiotics E. faecalis had a significant effect on reducing total VFA. It can be concluded that Supplementation of probiotic E. faecalis from maggot H. illucens at the level of 0.5 and 1% has not been able to work optimally on rumen fermentability and digestibility. Supplementation of probiotic E. faecalis at the levels of 0.5% and 1% did not interfere with the fermentation activity in the rumen.
Keyword: digestibility, E. faecalis, fermentability, in vitro, probiotics

Judul: Pengenalan Aksara Jawa Tulisan Tangan dengan Menggunakan Ekstraksi Fitur Zoning dan Klasifikasi K-Nearest Neighbour
Abstrak: Various studies on traditional script recognition continued to be developed using various methods. One of them is handwritten Javanese script recognition. This research aims to determine the accuracy of the Zoning Feature Extraction and K-Nearest Neighbour Classification method. The data used in this this research are handwritten Javanese script from 20 different peoples. Each of character images will be transformed into 120 x 120 pixels dimension and will undergo the thinning method. The feature extraction method used is the combination of the zoning method ICZ-ZCZ with the number of zones are 4, 6, 8, 9, 10, 12, 15, 16, 18, 20 and 24. K-Nearest Neighbour is used as the classifier with k values are 1, 3, 5 and 7. The highest accuracy was obtained on 12 zones with k = 1 on K-Nearest Neighbour with a value of 71.5%.
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