Judul: Respon Fisiologis dan Tingkah Laku Domba Garut dengan Pakan Limbah Tauge atau Kangkung Kering sebagai Pengganti Rumput
Abstrak: Garut sheep is commonly reared in West Java and usually fed with grass as the main feed and energy source. However, this grass has low quality and seasonally available, therefore should be an alternative feed such as mung bean sprout waste and dried kangkung. This research aimed to study sheep physiology and behavior as a response to diet and environment. This research used nine garut sheep (I0) with three treatments and replicates. The treatments were P0 (50% grass+50% consentrate), P1 (50% mung bean sprout waste+50% concentrate) and P2 (50% dried kangkung+50% concentrate). Physiological response data were analyzed by analysis of variance (ANOVA) and tested by Tukey post test method, while behavior frequency data were analyzed by Kruskal-Wallis test. Result showed that diet treatment did not affect (P>0.05) the heart and respiration rate, but significantly affected (P<0.05) on rectal temperature. All behaviors parameters were not significantly different (P>0.05) except for resting, grooming, licking strange object, and locomotion were higher in P1 than P0 and P2.
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Judul: Respon Fisiologis dan Tingkah Laku Domba Garut Jantan dengan Pakan dan Manajemen Pemberian Pakan Berbeda
Abstrak: Improving feeding and managament for sheep it is normally because of improving their performance. Thereby it can be effected to their physiological response and behaviour. This research aimed to compare physiological response and behaviour of garut rams with feed and different management feeding. This research used 16 garut rams (I0) with 2 factors and 4 replicates. First factors is type of feed R1 (40 % grass + 60% concentrate 1) dan R2 (40% mung bean sprout waste + 60% concentrate 2) and the second factors is management feeding in the morning (P) and management feeding in the afternoon (S). Physiological response were observed such as respiration, heart rate, and rectal temperatures. Behaviour were observed such as ingestive behaviour, licking and biteing other goods, resting, eliminasi, vokalisasi, and agonistic. Physiological response and behaviour data were analyzed by analysis of variance (ANOVA). The result shows that heart rate effected by feed. Sheep which is given feeding mung bean sprout waste have a higher value on heart rate (P<0.01). Rectal temperature efected by feed and management. Sheep which is given feeding mung bean sprout waste (P<0.01) and management feeding in the afternoon (P<0.05) have a higher value on rectal temperatures. Management feeding factors in morning of measuring ingestive behaviour have a higher value (P<0.05) but for resting behaviour, management feeding factors in the afternoon have a higher value (P<0.01). The grass have a higher value for eliminative in daylight periode (P<0.05). Both of factors were not significantly different (P>0.05) for measuring all of behaviour garut rams in the allnight periode.
Keyword: physiological response, garut rams

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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