Judul: Introduction of Serine Green Fluorescent Protein (sGFP) gene into pyricularia grisea Race dc4 isolated from digitaria ciliaris using agrobacterium tumefaciens-mediated genetic transformation
Abstrak: Pyricularia grisea is a rice plant pathogen has been known as the agent causing blast disease. Serin Green Fluorescent Protein (sGFP) gene have been used to monitorised gene expression and virulence of blast-causing fungi that has implication for fungal cell study. This research aimed to introduce sGFP gene into genome of P. grisea dc4 from D. ciliaris using A. tumefaciens. Plasmid sGFP was inserted into A. tumefaciens by triparental mating method (TPM). Genetic transformation was performed by co-cultivating spore P. grisea dc4 with A. tumefaciens LBA4404–pCAMB-sGFP. Pyricularia grisea dc4 transformant was selected by using selection medium that contain hygromycin. The growth test of non transformant P. grisea on OA medium 300 μg/ml hygromycin is slower than P. grisea transformant sGFP that increased rapidly from day 7 until day 21. The integration of sGFP gene into genome was confirmed by PCR using sGFP’s spesific primer pair, sGFP-Nos terminator primer pair and β-Tubulin primer pair as internal control. Expression of sGFP from P. grisea dc4 transformant was detected using fluorescent microscope with a 515 nm bandpass emission filter. Microscopic observation show that the growth of P. grisea dc4 transformant spores started from the basal area which will become the germ tube formation. The transformant P. grisea dc4 showed the ability to infect and to colonize within the host, that is showed by the hyphae fluorescence. This result clearly indicated that P. grisea dc4 which was taken from grass have the ability to infect on susceptible rice varities.
Keyword: Agrobacterium tumefaciens-mediated transformation, Digitaria ciliaris, Pyricularia grisea dc4, Serine Green Fluorescent Protein.

Judul: Transformasi Genetik Pada Padi Indica Menggunakan Metode Infeksi Awal Jaringan Skutelum
Abstrak: Genetic transformation of indica rice cultivar such as Ciherang, Inpari6, and Bio110 were successfully use Agrobacterium tumefaciens strain AGL1 contains SNAC1 gene and A.tumefaciens strain EHA105 contains HVA1 gene which introduced to scutellum tissue. The binery vector in both of A.tumefaciens contain hygromycin fosfotransferase (hpt) as selection marker gene. Efficiency of transformation for Ciherang was 5,69%, and for Inpari6 was 1,6%-5,88%. After regeneration stage, this transformation was produced 18 putative transgenic plants which resistance for hpt selection agent. The PCR analysis for transgenic plants shown that 14 plants contain hpt gene, consist of 7 Ciherang rice and 7 inpari6 rice.
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Judul: Truncated Stop Loss as a Optimal Solution of Reinsurance Agreement in One-Period Model
Abstrak: Reinsurance is one of risk management efforts of an insurance company, which is called the cedent. Similar with insurance, reinsurance constitutes the cedent to pay the premium. We consider one-period reinsurance model and derive a rule which minimizes the ruin probability of the cedent for a fixed reinsurance premium. Therefore, it is needed to construct an optimal reinsurance agreement for the cedent. The premium is calculated according to four different principles, i.e. economic, generalized zero-utility, Esscher, and mean-variance principles. Stop loss is a reinsurance contract which protect the insurance company for losses that exceed a certain amount. The result of this research shows that a truncated stop loss is an optimal agreement in the class of all reinsurance contracts with various premiums calculated using above mentioned principles.
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