Judul: Identifikasi Morfologi dan Marka Genetik Terpaut Sifat Tidak Berbunga Jantan pada Mutan Pisang Kepok
Abstrak: Bananas is an important horticultural commodity in Indonesia. However, the improvement of production capacity of cooking banana is hampered by Blood Disease Bacterium (BDB). Cooking banana such as kepok banana is very important commodity in several areas in eastern part of Indonesia for cash crop as well as for daily consumption. Since late 90s, production of kepok banana from Kalimantan drastically declined as a result of devastating outbreak of BDB. As a result many farmers are losing their main source of income. The disease is quickly spread at about 100 km distance per year as the bacterium is insect transmitted. Disease infection could be reduced by avoiding direct contact between insect vector and male bud by breaking peduncula supporting male bud or by cultivating male budless kepok banana mutant. Recently Centre for Tropical Horticultural Studies (PKHT) at Bogor Agricultural University (IPB) already collected several male budless kepok banana mutants with good eating quality named Unti Sayang (US-1). However, the genetic stability of these mutants is still unknown. Therefore, discovering DNA markers related to male budless character in kepok banana is very important to establish a stable mass propagation system for male budless character on kepok banana, and valuable for science to elucidate male budless mechanism in banana. The objectives of this research were to identify morphological characters and RAPD and ISSR markers and develop Pistillata and Agamous gene based moleculer marker related to male budless characters in kepok banana mutant Unti Sayang (US-1). Identification of morphological characters were carried out based on the International Plant Genetic Research Institute (IPGRI) descriptor for banana. The results showed that there is no morphological variation among wild type, mutants and revertrant other than a male bud character. The revertrant mutants were found among planlets derived from the sixth sub culture and second generation of suckers. Polymerase Chain Reaction (PCR) was used to amplify DNA of 24 plants derived from the first and sixth sub cultures using 20 RAPD and 12 ISSR primer combinations. Those RAPD and ISSR primers produced monomorphic DNA bands with the amount ranged from 2 to 6 and 6 to 8 per plant, respectively. No polymorphism was found between male budless mutants and revertrant mutants. The absence of genetic variation among wild type, mutants and revertrants has been reported in several cases of molecular marker analysis in in vitro cultured banana, although there was a variation in their morphology. Based on the PCR analysis, it was suggested that there was no genetic change between wild type and mutants. However, it is possible that some changes might occurred as point mutation located outside of the priming sites. Male budless characters was suggested to be controlled by Pistillata gene that control petal and stamen formation and Agamous gene for karpel formation. Therefore, the primers based Pistillata and Agamous genes were developed using Musa GeneBank database. PCR analysis using those primer combination have also produced no polymorphic bands among the analyzed banana plants. Carefull nucleotide sequence analysis of both DNA fragments showed three single nucleotide polymorphism between wild type and revertrant mutants at the position of
Keyword: ISSR, Male budless kepok banana, Pistillata, RAPD

Judul: Diversity Analysis of Banana (Musa spp.) Based on RAPD and ISSR Molecular Markers
Abstrak: Persilangan kedua tetua pisang, Musa acuminata (AA) dan Musa balbisiana (BB), menghasilkan keragaman tingkat ploidi yang menghasilkan variasi sifat pada setiap kultivar pisang. Analisis keragaman pisang dengan pendekatan morfologi cenderung lebih mudah untuk dilakukan tetapi dapat karakter tersebut dipengaruhi oleh lingkungan. Oleh karena itu, pada penelitian ini perlu dilakukan analisis keragaman pisang menggunakan marka molekular RAPD dan ISSR untuk mendeteksi perbedaan pada tingkat DNA. Metode yang dilakukan yaitu pengamatan morfologi, isolasi DNA kultivar pisang, amplifikasi DNA menggunakan primer RAPD dan ISSR, dan pengolahan data hasil PCR dengan scoring dan software PAST 4. Analisis keragaman genetik menggunakan marka RAPD dan ISSR pada delapan kultivar pisang menghasilkan 164 pita DNA dengan rata-rata persentase pita polimorfik 78.9%. Jarak kekerabatan terjauh dari seluruh kultivar pisang yang dipelajari terdapat pada pisang Lampung yang merupakan satu-satunya sampel bergenom diploid., Crossing the two parental bananas, Musa acuminata (AA) and Musa balbisiana (BB), results in a diversity of ploidy levels that produce variations in traits in each banana cultivar. Analysis of banana diversity with a morphological approach tends to be easier to do, but the environment can influence the character. Therefore, in this study, it is necessary to analyze banana diversity using RAPD and ISSR molecular markers to detect differences at the DNA level. The methods used were Morphological observation, Isolation of banana cultivar DNA, DNA amplification using RAPD and ISSR primers, and data processing of PCR results with scoring and PAST 4 software. Analysis of genetic diversity using RAPD and ISSR markers on eight banana cultivars produced 164 DNA bands with an average percentage of polymorphic bands of 78.9%. The farthest relationship distance of all studied banana cultivars was found in the Lampung banana, the only diploid genome sample.
Keyword: morfologi, PCR, kekerabatan, marka molekuler, polimorfisme

Judul: Hydroxyapatite-Chitosan Composite Coating on Stainless Steel 316 to Improve Corrosion Resistance
Abstrak: Metals that were used in bone implantation should have good corrosion resistance that were not easily corroded when implanted into the body. Metal corrosion resistance of stainless steel 316 can be enhanced through the metal coating by hydroxyapatite-chitosan composite with composition ratio of hydroxyapatite (gram) and chitosan 3% (mL) 0.2:0, 0.2:0.1, 0.2:0.5, 0.2:1.0, and 0.2:1.5. Besides improving the corrosion resistance of metal, the presence of chitosan also maintain the position of hydroxyapatite on metal surfaces. Corrosion resistance increases with increasing amount of chitosan added and the best composition of hydroxyapatite-chitosan composite was 0.2:1.0.
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