Judul: Desain Strategi Manajemen Risiko Iklim di Indonesia
Abstrak: Komitmen Indonesia dalam melawan perubahan iklim, terlihat pada keikutsertaan dalam menandatangani Perjanjian Paris yang berisikan pembatasan kenaikan suhu global tidak lebih dari 2 oC dan mengeluarkan peraturan-peraturan yang berkaitan termasuk Permen LHK No. P.7 Tahun 2018 tentang pedoman kajian kerentanan, risiko, dan dampak perubahan iklim. Penelitian ini menggunakan tinjauan literatur sistematis dalam mendapatkan informasi dampak dan risiko iklim di berbagai tempat dalam menyusun data indikator sesuai Permen tersebut, lalu menyusunnya menjadi beberapa kebijakan; serta MCA digunakan dalam menentukan skala prioritas dari kebijakan yang dibuat. Pencarian literatur menggunakan Scopus menggunakan string yang telah ditentukan dengan rentang tahun 2016-2024 untuk melihat respons terhadap Perjanjian Paris. Kebijakan yang didapatkan pada penelitian ini, yaitu asuransi iklim pada sektor pertanian, peningkatan kapasitas, peningkatan kesejahteraan masyarakat pesisir, Pemaksimalan ruang terbuka hijau, dan pemberdayaan masyarakat adat, dengan peningkatan kapasitas menjadi skala prioritas utama pada penilaian MCA. Saran untuk desain kebijakan kedepannya harus multi sektor, keberlanjutan, dan promosi., Indonesia's commitment to fighting climate change can be seen from its participation in signing the Paris Agreement which contains limits on global temperature rise to no more than 2 OC and issued related regulations including Minister of Environment and Forestry Regulation No. P.7 of 2018 concerning guidelines for assessing the vulnerability, risks and impacts of climate change. This research uses a systematic literature review to obtain information on climate impacts and risks in various places in compiling indicator data according to the Ministerial Regulation, then compiling it into several policies; and MCA is used in determining the priority scale of the policies being made. Literature search using Scopus string which has been determined with a range of 2016-2024 to see the response to the Paris Agreement. The policies obtained in this research are climate insurance in the agricultural sector, increasing capacity, improving the welfare of coastal communities, maximizing green open space, and empowering indigenous communities, with increasing capacity being the main priority scale in the MCA assessment. Suggestions for future policy design must be multi-sectoral, sustainable and promotional.
Keyword: Climate Change Impact, Climate Risk, Climate Risk Management, MCA

Judul: Tinjauan Hasil Kajian Kerentanan dalam Pengembangan Strategi Adaptasi di Indonesia Terkait Perubahan Iklim
Abstrak: The impact of climate changes going increasingly worrying. It put Indonesia as one of the most vulnerable countries affected by climate change. This study was conducted by reviewing the studies that have been done by DNPI focused on adaptation strategies to climate change in Indonesia. Especially to the vulnerability of food, health, and in coastal areas, which is expected to anticipate losses due to the impact of greater climate change. The development of adaptation strategies is a top priority that must be done, and the role of government in this case is The National Council on Climate Change (NCCC), it is necessary to formulate, coordinate and control the policies that is consistent with climate change adaptation. The cooperation of all parties to participate in controlling climate change makes it especially important considering the process of socialization and adaptation. It requires a synergistic follow-up between the government, private sector, NGOs to the general public. Adaptation strategies of the results of this study is also expected to reduce climate refugees due to climate change.
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Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.