Judul: Struktur dan komposisi tegakan serta sistem perakaran tumbuhan pada kawasan karst di Taman Nasional Bantimurung-Bulusaraung, Resort Pattunuang-Karaenta
Abstrak: Plants in Karst Area have different characteristics and distinctive when compared with plants of other forest ecosystem. The purpose of research is to assessing the composition and stand structure and root system of plants in karst areas in Bantimurung-Bulusaraung National Park, Resort Pattunuang-Karaenta. This observation plots determined by purposive sampling based on the representation of the stand. Three sample plots lines with the size of 20 m x 100 m each were constructed in the research site. The research was spread out on four habitats that are on hill base, slopes, summit with considerable soil cover, and summit with very little soil cover. The number of species on the research area is amounted 108 species of seedlings and understorey plants, 108 species saplings, 63 species poles and 78 species trees. Horizontal structure inverted J-shaped and vertical structure of the stand consist of four strata (B, C, D, and E strata). Overall, the spatial distribution patterns have a clumped distribution pattern. Uniform distribution pattern is only found in Palaquium obovatum species of pole on slopes and Dracontomelon dao species of tree on base. Pterospermum celebicum has the highest value of shoot-root ratio on the hill base, index root binding highest value with category of moderate (1.5-3.5) on hill base and index root anchoring highest value with the high category (>1.0) on summits with considerable soil cover.
Keyword: Bogor Agricultural University (IPB), stand structure, root system, stand composition

Judul: Komposisi Jenis dan Struktur Tegakan di Hutan Penelitian Haurbentes Jasinga, Kabupaten Bogor
Abstrak: The Haurbentes Special Purpose Forest Area which functioned as a reaserch forest has various types of stands. This study was purposed to obtain informations on the species composition and stand structure in Harbuentes Research Forest. Unit samples taken by using purposing sampling method on 20 ha Haurbentes forest area. Observed variables in this study are number of vegetation individuals for seedlings and saplings, also diameter and number of individuals vegetation level from trees which diameter greater than 10 cm in 10 m x 50 m pots with seedlings, saplings and tree measuring on each type. Estimation of forest stands potential calculated with Dipterocarpaceae volume estimation and Berkhout linier transformation equation. Based on the observation, biodiversity index forest (H') in Haurbentes Research Forest for all levels of growth were categorized as moderate classification since it had biodiversity index forest values between 1,5 to 3,5. The species richness for seedling and trees growth rate were categorized as low classification and the sapling growth rate were categorized as high classification. The highest number of plants was founded in sapling growth rate and the lowest number of plants was founded in seedling and trees growth rate, with the value of 20 species of seedling, 51 species of saplings and 15 species of trees. Species of Shorea mecistopteryx was the dominant species at every stages of growth because these species are found in large numbers and spread almost evenly throughout the area. The stand structure of Haurbentes Forest was characterized as the larger the size of the diameter of the rod the more reduced the number of individuals of the species (the inverted "J" letter curve graphic). Based on 2012 tree treatise at the Haurbentes research forest in the area of 20 ha were found 2987 trees. Total of Dipterocarpaceae wood volume potential obtained in Haurbentes research forest is 592,77 m3 or 29,64 m3/ha.
Keyword: stand structure, species composition

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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