Judul: Perkembangan Embrio Mencit (Mus musculus albinus) Pada Inkubator CO2 Termodifikasi
Abstrak: The present study was conducted to investigate effectiveness of a simple portable CO2 incubator for embryo production (IVEP) of mouse embryo by in vitro. In an attempt to determine the culture system was using CO2 gas produced from effervescent powder that approaches a standard CO2 incubator condition (5- 6%) (B), than embryos were examine at condition with a dose of effervescent formulations below (A), and upper (C) dose of normal condition. In vitro development rates of mouse embryos cultured in the portable CO2 incubator were not significantly different from the standard (control) CO2 incubator in a variety of treatment, which shows that mouse embryos can growth as well as in these conditions. Although there were no significant differences between the two systems in terms of embryos development, but there was a significantly differences for CO2 gas generated in situ in three treatments were compared with standard CO2 incubator (control). Concentration of CO2 gas in situ in a portable CO2 incubator was higher (P<0.05) than control (5.02 ± 0.08366%) for C (6.74 ± 0.24083%) treatment, but lower than control for A (2.22 ± 0.08366%) and B (4.46 ± 0.05477%) treatments. The present study indicates that a portable CO2 incubator is a useful device for in vitro mouse embryos production. Further studies using this portable incubator system are needed to determine the optimum levels of O2, CO2, and air pressure.
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Judul: Viabilitas Embrio Mencit (Mus musculus albinus) Vitrifikasi setelah Kultur In Vitro dalam Inkubator CO2 Portable.
Abstrak: Inkubator CO2 portable dapat digunakan untuk kultur embrio selama proses transportasi. Vitrifikasi merupakan salah satu metode kriopreservasi untuk penyimpanan embrio dalam waktu yang lama. Penelitian bertujuan untuk mengevaluasi tingkat perkembangan dan viabilitas embrio vitrifikasi setelah kultur in vitro dalam inkubator CO2 portable. Embrio dikoleksi dari mencit pada hari ke-3 pasca fertilisasi yang telah disuperovulasi menggunakan PMSG dan hCG. Embrio dibagi ke dalam kelompok vitrifikasi dan tanpa vitrifikasi (sebagai kontrol). Vitrifikasi dilakukan menggunakan krioprotektan etilen glikol 15% + DMSO 15%, dihangatkan (warming) secara bertahap dalam sukrosa 0.5, 0.25 dan 0.1 M, serta dikultur in vitro selama 48 jam. Evaluasi berupa tingkat perkembangan embrio dan viabilitas dengan pewarnaan vital Hoechst–propidium iodide. Hasil yang diperoleh menunjukkan bahwa embrio pasca vitrifikasi tidak memperlihatkan penurunan kualitas secara morfologi. Tingkat perkembangan embrio tidak menunjukkan perbedaan antara embrio vitrifikasi dan kontrol (P>0.05). Embrio yang divitrifikasi mampu mencapai tahap hatching dan hatched. Total sel dan persentase sel hidup pada embrio vitrifikasi tidak berbeda dengan embrio kontrol, yaitu berturut-turut 112.6±27.7 dan 95.8±3.3% untuk embrio vitrifikasi serta 100.8±17.2 dan 97.3±3.2% untuk kontrol (P>0.05). Dapat disimpulkan bahwa embrio mencit setelah vitrifikasi memiliki tingkat perkembangan dan viabilitas yang baik setelah dikultur in vitro dalam inkubator CO2 portable.
Keyword: embrio mencit, inkubator CO2 portable, viabilitas, vitrifikasi

Judul: Formulasi TSP (Traveling Salesman Problem) menggunakan ILP (Integer Linear Programming)
Abstrak: TSP is a journey of salesman f?om his origin town (place) to visit n-cities (places) only at once, then return to his origin town (place) with a minimum total distance. Its purpose is to minimize the operating expense that is released by the company. Vehicle route at TSP in the form of a Hamiltonian cycle, that is closed path containing all nodes at the graph which representing a network walk. Its problem is to determine a journey route which is feasible (possibly can be passed by) in such a way the vehicle travelled distance passed the route is minimum.
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