Judul: Kualitas tidur mahasiswa Institut Pertanian Bogor.
Abstrak: Tidur merupakan aktivitas penting dalam kehidupan manusia. Tidur adalah keadaan di mana otak tidak mengolah informasi dari neuron sensorik dan tidak memberi perintah ke neuron motorik. Kualitas tidur seseorang dapat ditentukan dengan metode Pittsburgh Sleep Quality Index (PSQI). Kualitas tidur terdiri atas tujuh komponen, yaitu: kualitas tidur subyektif, tidur laten, lama tidur, efisiensi tidur, gangguan tidur, pemakaian obat tidur dan disfungsi siang hari. Ketujuh komponen dikuantifikasi dan total skor yang didapat disebut nilai PSQI. Jika seseorang mempunyai nilai PSQI ≤ 5 maka ia mempunyai kualitas tidur baik, dan jika seseorang mempunyai nilai PSQI > 5 maka ia mempunyai kualitas tidur yang buruk. Tujuan penelitian ini untuk mengetahui kualitas tidur mahasiswa Institut Pertanian Bogor. Proporsi mahasiswa S1 laki-laki yang berkualitas tidur baik (15.48%) sama dengan proporsi mahasiswa S1 perempuan yang berkualitas tidur baik (15.36%). Begitu juga proporsi mahasiswa pascasarjana laki-laki dan perempuan yang berkualitas tidur baik adalah sama yaitu (25% dan 28.13%). Tanpa memandang jenis kelamin, proporsi mahasiswa S1 yang berkualitas tidur baik adalah 15.41%, sedangkan proporsi mahasiswa pascasarjana yang berkualitas tidur baik adalah 27.26%.
Keyword: sleep, Pittsburgh Sleep Quality Index (PSQI), student

Judul: Studi Kualitas Tidur Pegawai Institut Pertanian Bogor
Abstrak: Sleep is an important activity undertaken by humans and spend nearly a third of the human life time. Sleep is a condition in which the brain does not process information from sensory neurons and does not give orders to the motor neurons. Sleep quality of person can be determined using the Pittsburgh Sleep Quality Index (PSQI). The data of this research were obtained by giving questionnaires contain personal data and questions about the components of sleep quality during the past month There are seven components of sleep quality, subjective sleep quality, sleep latency, sleep duration, sleep efficiency, sleep disturbances, use of sleeping medication, and daytime dysfunction. The purpose of this study was to determine the sleep quality of employee of Bogor Agricultural University. Value of PSQI obtained from the calculation of the seven components, if someone has a value of PSQI < 5 then he has good sleep quality, and if someone has value of PSQI >5 then he has poor sleep quality. The purpose of this study is to determine the sleep quality of employee of Bogor Agricultural University. The proportion of male employee who had good quality sleep were equal to the proportion of female who had good quality sleep. As much as 48.27% of the total sample of employees have good sleep quality. As much as 62,5% of employees over the age of 51 years have poor sleep quality.
Keyword: 

Judul: Gene Editing  of Two Cyanide Synthesis-related Genes in Cassava using CRISPR/Cas9
Abstrak: Cassava (Manihot esculenta) contain toxic quantities of cyanogenic glucoside. Reducing the potentially toxic cyanogenic glucoside levels in cassava could be achieved by gene editing through Clustered Regularly Interspaced Short Palindromic Repeats – CRISPR associated protein 9 (CRISPR/Cas9), a high- precision genome editing technique in plants. Two cassava UDP- glucosyltransferase (UGT) paralogs, UGT85K4 and UGT85K5, are the genes catalyzing the synthesis of cyanogenic glucosides in plants. The study aimed to construct the CRISPR/Cas9 recombinant vector carrying single guide RNA (sgRNA) to edit UGT85K4 and UGT85K5 genes, and transform sgRNA CRISPR/Cas9 construct into KU50 cassava calli. Five sgRNAs were designed, considering the on/off target efficiency score and GC content. Those sgRNAs were ligated into pRGEB32 plasmid and introduced to competent Escherichia coli DH5a. The PCR colony and DNA sequencing were performed to verify the sgRNA insert. The amplicons showed the expected band size of recombinant vector (180 bp). Based on sequencing results, only one sgRNA was successfully ligated into the plasmid that will be used to edit the UGT85K5 gene. The sgRNA CRISPR/Cas9 construct in pRGEB32 plasmid was successfully transformed into Agrobacterium tumefaciens and introduced into KU50 cassava calli through co-cultivation. The overgrowth of Agrobacterium occurred after the co-cultivation phase.
Keyword: CRISPR/Cas9, Cyanogenic glucoside, Gene editing, sgRNA