Judul: The Relationship of Percent Body Fat, Physical Activity with Physical Fitness of State High School Girls 1 Merawang
Abstrak: Masa remaja merupakan perubahan dari kanak-kanak menuju dewasa. Permasalahan yang sering terjadi dikalangan remaja putri adalah kurang gizi dan pola makan yang salah. Aktivitas fisik juga sebagai faktor yang mempengaruhi tingkat kebugaran jasmani. Tujuan penelitian ini adalah untuk menganalisis hubungan antara persen lemak tubuh, tingkat kecukupan zat gizi dan aktivitas fisik dengan kebugaran jasmani pada remaja perempuan SMA Negeri 1 Merawang. Desain penelitian ini adalah cross sectional dengan subjek berjumlah 106 orang. Pengumpulan data kebugaran jasmani subjek melalui tes harvard, persen lemak tubuh pengukuran menggunakan bioelectrical impedance analysis (BIA). Data aktivitas fisik diperoleh dari recall aktivitas fisik di hari sekolah dan hari libur. Data asupan zat gizi diperoleh dari food recall 24 jam di hari sekolah dan hari libur. Hasil penelitian menunjukkan bahwa tingkat kecukupan zat gizi makro sebagian besar subjek termasuk dalam kategori defisit tingkat berat. Tingkat kecukupan zat gizi mikro sebagian besar subjek termasuk dalam kategori kurang. Uji korelasi Spearman pada persen lemak tubuh dengan kebugaran jasmani menunjukkan ada hubungan negatif (p= 0,005, r=-0,272), tidak terdapat hubungan yang signifikan antara tingkat kecukupan zat gizi, aktivitas fisik dengan kebugaran jasmani (p>0,05)., Adolescence is a change from childhood to adulthood. Problems that often occur among young women are undernourishment and the wrong diet. Physical activity is also a factor that affects the level of physical freshness. The purpose of this study was to analyze the relationship between body fat percentage, nutritional adequacy levels and physical activity with physical fitness in adolescent girls in State High School 1 Merawang. The design of the study was cross-sectional with 106 subjects. Data was collected on the subjects' physical fitness through Harvard tests, percent body fat measurements using bioelectrical impedance analysis (BIA). Physical activity data is obtained from the recall of physical activity on school days and holidays. Nutritional intake data is obtained from a 24-hour food recall on school days and holidays. The results showed that the adequacy levels of the macronutrients of most of the subjects fall into the category of weight deficit levels. The adequacy levels of micronutrients of most subjects fall into the lesser category. Spearman's correlation test on the percent of body fat with physical fitness showed a negative association (p= 0.005, r=-0.272), there was no significant association between levels of nutritional adequacy, physical activity, and physical fitness (p>0.05).
Keyword: Adequacy of Nutrients, Fitness, Percent Body Fat, Physical Activity

Judul: The Relationship between Fruit Vegetable Consumption Habits and Physical Activity with Nutritional Status and Physical Fitness of Students at SDN 1 Cijeungjing
Abstrak: Status gizi kurang dan lebih merupakan salah satu masalah gizi yang banyak dihadapi pada usia anak sekolah dasar. Status gizi dapat dipengaruhi oleh berbagai faktor seperti pengetahuan gizi, aktivitas fisik, kebugaran, dan kebiasaan makan. Tujuan penelitian ini adalah menganalisis hubungan kebiasaan konsumsi sayur dan buah serta aktivitas fisik dengan status gizi dan kebugaran jasmani siswa di SDN 1 Cijeungjing. Penelitian ini dilaksanakan pada bulan Oktober-November 2022 pada 58 subjek dengan desain cross sectional study. Pengambilan data dilakukan melalui pengukuran tinggi badan dan berat badan, wawancara, harvard step test, pengisian kuesioner meliputi karakteristik subjek dan keluarga, PAQ-C (Physical Activity Questionnaire for Children), dan FFQ (Food Frequency Questionnaire). Sebagian besar subjek berjenis kelamin laki-laki (72,4%) dengan status gizi normal (79,3%). Hasil analisis menunjukkan terdapat hubungan yang signifikan (p<0,05) antara kebiasaan konsumsi sayur dan buah dengan aktivitas fisik (p-value=0,050;r=-0,129). Terdapat hubungan yang signifikan (p<0,05) antara aktivitas fisik dengan kebugaran jasmani (p-value=0,029;r=0,288). Namun, tidak terdapat hubungan yang signifikan (p>0,05) antara kebiasaan konsumsi sayur dan buah dengan status gizi (p-value=0,338;r=0,128). Tidak terdapat hubungan yang signifikan (p>0,05) antara aktivitas fisik dengan status gizi (p-value=0,142;r=0,195). Tidak terdapat hubungan yang signifikan (p>0,05) antara kebiasaan konsumsi sayur dan buah dengan kebugaran jasmani (p-value=0,333;r=-0,129)., Underweight and overweight are one of the most common nutritional problems faced by elementary school children. Nutritional status can be influenced by various factors such as nutritional knowledge, physical activity, fitness, and eating habits. The aim of this study was to analyze the relationship between fruit vegetable consumption habits and physical activity with the nutritional status and physical fitness of students at SDN 1 Cijeungjing. This study was conducted in October-November 2022 on 58 subjects with a cross-sectional study design. Data collected by measuring height and weight, interviews, harvard step test, filling out questionnaires including subject and family characteristics, PAQ-C questionnaire (Physical Activity Questionnaire for Children), and FFQ questionnaire (Food Frequency Questionnaire). Most subjects were male (72.4%) with normal nutritional status (79.3%). The results of the analysis showed that there was a significant correlation (p<0.05) between fruit vegetables consumption habits with physical activity (p-value=0.050;r=-0.129). There was significant correlation (p<0.05) between physical activity with physical fitness (p-value=0.029;r=0.288). However, there was no significant correlation (p>;0.05) between fruit vegetables consumption habits with nutritional status (p-value=0.338;r=0.128). There was no significant correlation (p>0.05) between physical activity with nutritional status (p-value=0.142;r=0.195). There was no significant correlation (p>0.05) between fruit vegetables consumption habits with physical fitness (p-value=0.333;r=-0.129).
Keyword: fruit vegetables consumption habits, nutritional status, physical  activity, physical fitness

Judul: Isolasi Konstruksi dan Rekombinasi Gen Pengkode Reverse Transcriptase asal Simian Retrovirus-2 (SRV-2)
Abstrak: The availability of reverse transcriptase enzym as a valuable component for Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique has been providing by foreign companies. Therefore, it needs more cost and time to provide it. This study is an early step to produce in house reverse transcriptase rekombinant enzym from SRV-2. The purpose of this study was isolated, constructed, and rekombined SRV-2 reverse transcriptase gene to entry clone and destination vector. Isolation was done by PCR amplification technique using three pairs of primers, which are RT I (3282-3683), RTII (3261-3900), and RTIII (3169-4133). Construction and recombination in this study used Gateway method from Invitrogen. SRV-2 reverse transcriptase gene was isolated in this study with A-549 cell inoculated SRV-2 as source of virus. This gene amplify by PCR technique and resulted specific band for RTI 401 bp, RTII 639 bp, and RTIII 964 bp. Analysis of pENTR/SD/TOPO and pDEST17 indicated SRV-2 reverse transcriptase gene code has already inserted to this plasmid. This result indicated SRV-2 reverse transcriptase gene have isolated, constructed, and recombined to entry clone and destination vector.
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