Judul: Pemberitaan peran wanita pada media massa cetak untuk wanita
Abstrak: Tujuan penelitian adalah mempelajari freuensi dan volume isi media massa cetak untuk wanita yang terbit di Jakarta selama Januari - Desember 1992. Aspek yang dilihat mencakup mesalah peran wanita di dalam keluarga, masalah peran wanita di luar keluarga, dan perbandingan frekuensi dan volume masalah peran wanita di dalam dan di luar keluarga.
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Judul: Telaahan peranan kerja wanita di sektor informal : studi kasus wanita pedagang sayur dan buah di Pasar Pondok Labu dan Pasar Mede di Kecamatan Cilandak, Jakarta Selatan
Abstrak: Dari seluruh angkatan kerja Indonesia, sekitar 53 persen bekerja di sektor informal, diantaranya sekitar 80 persen angkatan kerja wanita. Selama periode 1971 1980 diketahui terjadi peningkatan angkatan kerja di sektor informal. Adapun kenaikan tersebut terjadi didaerah perkotaan dengan kenaikan rata-rata sekitar 11,1 persen, sementara di desa hanya 1,1 persen (BPS, 1980). Penelitian Peranan Wanita disektor informal secara umum telah banyak dikemukakan, tetapi cenderung hanya berkisar pada aspek ekonominya saja serta lebih banyak menyoroti kasus-kasus pertanian di desa atau kasus-kasus pedagang makanan (Street Food) di kota; sementara untuk kasus-kasus pedagang sayuran dan buah-buahan masih belum banyak dilakukan. Padahal dalam kenyataannya fenomena tersebut sangat banyak dijumpai didaerah perkotaan yang umumnya dilakukan oleh wanita migran. Tujuan penelitian ini untuk (1) mempelajari karakteristik wanita di sektor informal khususnya wanita pedagang sayur dan buah, (2) Mempelajari peranan kerja wanita pedagang sayur dan buah terutama yang meliputi tingkat kontribusi pendapatannya dan curahan waktu berdagang, (3) mengetahui permasalahan yang dihadapi, dan strategi pencarian nafkah yang dilakukan...
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Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.