Judul: Gaya Pengasuhan Ibu, Interaksi Saudara Kandung, dan Penyesuaian Sosial Remaja Perempuan
Abstrak: Penyesuaian sosial adalah kemampuan sesorang untuk menyesuaikan diri dengan orang lain. Tujuan penelitian ini adalah menganalisis pengaruh gaya pengasuhan ibu dan interaksi saudara kandung terhadap penyesuaian sosial remaja perempuan anak tengah. Penelitian ini dilakukan di SMPN X Dramaga Bogor dengan sampel penelitian sebanyak 60 orang remaja perempuan anak tengah yang berusia 13-15 tahun. Gaya pengasuhan penerimaan berhubungan positif dengan dimensi kehangatan, kekuasaan, dan penyesuaian sosial, tetapi berhubungan negatif dengan dimensi persaingan. Gaya pengasuhan penolakan berhubungan positif dengan konflik dan persaingan, tetapi berhubungan negatif dengan penyesuaian sosial. Dimensi kehangatan dan kekuasaan berhubungan positif dengan penyesuaian sosial, dan dimensi persaingan berhubungan negatif dengan penyesuaian sosial. Penyesuaian sosial dipengaruhi oleh gaya pengasuhan ibu dan interaksi saudara kandung.
Keyword: gaya pengasuhan penerimaan-penolakan, interaksi saudara kandung, penyesuaian sosial, anak tengah, remaja perempuan

Judul: Gaya Pengasuhan Ibu, Interaksi Saudara Kandung, dan Kemandirian pada Remaja Anak Sulung Perempuan
Abstrak: Mencapai kemandirian merupakan salah satu tugas perkembangan yang harus dilakukan oleh remaja. Penelitian ini bertujuan untuk menganalisis hubungan gaya pengasuhan ibu (Parental Acceptance-Rejection), interaksi saudara kandung dan kemandirian pada remaja anak sulung perempuan. Penarikan contoh dilakukan secara purposive pada 60 remaja perempuan yang berusia 13-16 tahun di SMPN 2 Dramaga, Bogor. Contoh dalam penelitian ini dibedakan berdasarkan jenis kelamin adik yaitu remaja yang memiliki adik laki-laki sebanyak 30 orang dan adik perempuan sebanyak 30 orang. Hasil menunjukkan bahwa remaja mempersepsikan ibu melakukan pengasuhan penerimaan, interaksi kekuasaan saudara kandung memiliki indeks lebih tinggi daripada dimensi lain, dan kemandirian nilai memiliki indeks tinggi. Semakin tingginya penerimaan ibu akan meningkatkan kehangatan saudara kandung dan tingginya pengabaian ibu akan menurunkan kemandirian emosional pada remaja dengan adik perempuan. Pada remaja dengan adik laki-laki, penerimaan ibu akan meningkatkan persaingan dan interaksi kehangatan saudara kandung akan meningkatkan kemandirian emosional.
Keyword: anak sulung, gaya pengasuhan ibu (Parental Acceptence-Rejection), interaksi saudara kandung, kemandirian, remaja perempuan

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable