Judul: Isolasi, Identifikasi dan Karakterisasi Molekular Virus Hepatitis B pada Owa Jawa (Hylobates moloch) di Indonesia
Abstrak: Virus hepatitis B (HBV) dilaporkan tidak hanya dapat menginfeksi manusia, tetapi juga primata non-manusia, terutama kelompok kera besar seperti orangutan, gorila, simpanse, serta kera kecil dari famili Hylobatidae. Di antara spesies lain dalam genus, Hylobates moloch (owa jawa atau keperakan/owa jawa), yang dianggap sebagai salah satu spesies endemik Indonesia yang terancam punah, dilaporkan memiliki HBV dari strain mereka sendiri. Analisis HBV yang diisolasi dari owa yang terinfeksi dilakukan dalam penelitian ini untuk mengungkap keterkaitannya dengan data yang dipublikasikan. Sampel plasma dari sembilan owa jawa diperoleh sebagai bagian dari tujuan diagnostik. Hewan-hewan tersebut sebelumnya dinyatakan positif adanya hepatisis B-surface antigen (HBsAg) dengan enzyme-linked immunosorbent assay (ELISA). Sampel DNA diekstraksi dari sembilan sampel plasma tersebut untuk digunakan sebagai template untuk amplifikasi daerah Pre-S1 dengan teknik polymerase chain reaction (PCR) menggunakan primer semi degenerate yang dapat mengamplifikasi beberapa strain HBV. Produk PCR dari 457 - 459 pasangan basa kemudian dilakukan analisis polimorfisme panjang fragmen restriksi (RFLP) menggunakan enzim Bst2UI untuk mengevaluasi pola fragmen yang dibelah. Produk PCR diurutkan untuk analisis homologi lebih lanjut dan untuk membuat pohon filogenetik dari data sekuensing yang diperoleh dari penelitian dibandingkan dengan data yang dipublikasikan. Dua sampel plasma digunakan sebagai templat untuk amplifikasi seluruh wilayah menggunakan set 4 primer. Produk PCR dari 3192 pasangan basa diurutkan untuk analisis homologi lebih lanjut dan untuk membuat pohon filogenetik. Analisis pohon filogenetik untuk wilayah nukleotida Pra-S1 dan seluruh wilayah menunjukkan bahwa isolat owa jawa membentuk kelompok mereka sendiri, terpisah dari isolat primata bukan manusia lainnya.
Keyword: Virus hepatitis B, Pra-S1, genom utuh, owa jawa, PCR, Analisis filogenetik

Judul: Isolasi, Identifikasi dan Karakterisasi Molekular Virus Hepatitis B pada Owa Jawa (Hylobates moloch) di Indonesia
Abstrak: Hepatitis B virus (HBV) was reported not only able to infect human being, but also non-human primates, especially the great apes group such as orangutans, gorillas, chimpanzees, as well as the lesser apes from the family of Hylobatidae. Among other species within the genus, Hylobates moloch (owa jawa or silvery/ javan gibbons), which is considered as one of Indonesia’s endemic endangered species, has been reported to harbor HBV of their own strain. Analyses of HBV isolated from infected gibbons were done in this study to reveal their relatedness to the published data. Plasma samples from nine javan gibbons were obtained as part of diagnostic purpose. Those animals were previously tested positive for the presence of hepatisis B-surface antigen (HBsAg) by enzyme-linked immunosorbent assay (ELISA). DNA samples were extracted from these nine plasma samples to be used as templates for amplification of the Pre-S1 region by polymerase chain reactions (PCR) technique using semi degenerate primers that could amplify several strains of HBVs. The PCR products of 457 - 459 base pairs were then subject to restriction fragment length polymorphism (RFLP) analyses using Bst2UI enzyme to evaluate the pattern of cleaved fragments. The PCR products were sequenced for further homology analyses and to create phylogenetic tree of the sequenced data obtained from the study in comparison with published data. Two plasma samples were used as templates for whole region amplification using sets of 4 primer. The PCR products of 3192 base pairs were sequenced for further homology analyses and to create phylogenetic tree. Phylogenetic tree analyses for Pre-S1 nucleotide region and whole region showed that the javan gibbon isolates formed their own clusters, separate from the other nonhuman primate isolates.
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Judul: Development of Array-Based 3D Lighting Tool using MAXScript.
Abstrak: Array lighting uses a group of lights behaving and functioning as a single light. This method can be used to simulate the effect of global illumination in less time. In this research, Cylight, an array-based 3D lighting tool with tubular geometry, is developed using MAXScript. The image quality comparison to global illumination using Scanline and mental ray is conducted through an online survey using six 3D scenes. 98 respondents participated to determine the realistic level for each rendered scene with a value between 1 to 5. The rendering time is also measured. The results show that Scanline produces the best image quality, but not significantly different compared to Cylight. On the other hand, Cylight is significantly faster, taking less than 10% of Scanline's rendering time. Keywords: global illumination, fake GI, 3ds Max, array lighting, light arrays, computer graphics.
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