Judul: Keragaman Genetik Sengon (Falcataria moluccana Miq.) Resisten dan Rentan Terhadap Hama Boktor Berdasarkan Penanda Mikrosatelit.
Abstrak: Sengon (Falcataria moluccana Miq.) merupakan jenis tanaman cepat tumbuh yang multiguna, banyak ditanam pada hutan rakyat. Pola tanam monokultur memiliki risiko terserang hama penggerek batang seperti boktor (Xystrocera festiva). Salah satu cara untuk mengatasinya adalah dengan menanam pohon resisten yang dihasilkan dari program pemuliaan. Penelitian ini bertujuan untuk mengukur keragaman genetik sengon resisten dan yang terserng hama boktor berdasarkan penanda mikrosatelit. DNA diekstraksi menggunakan Dneasy plant mini kit dari QIAGEN, kemudian diamplifikasi menggunakan lima pasang primer mikrosatelit yaitu Pafa05, Pafa06, Pafa07, Pafa08 dan Pafa10. Hasil analisis menunjukkan bahwa hanya empat pasang primer yang dapat mengamplifikasi dengan baik yaitu primer Pafa06, Pafa07, Pafa08 dan Pafa10. Populasi sengon resisten memiliki tingkat heterozigositas tertinggi (He = 0.650), dibandingkan populasi sengon yang rentan boktor (He = 0.623). Populasi sengon resisten dan rentan terhadap hama boktor memiliki jarak genetik yang cukup dekat (0.160). Keragaman genetik kedua populasi tersebar sebanyak 94.09% di dalam populasi dan 5.91% di antara populasi.
Keyword: keragaman genetik, mikrosatelit, F. moluccana, rentan, resisten

Judul: Ekspresi Gen HSP 70 pada Sengon (Falcataria moluccana Miq.) Resisten dan Terserang Hama Boktor
Abstrak: Pohon sengon (Falcataria moluccana Miq.) merupakan salah satu pohon serba guna yang ada di Indonesia. Keberhasilan pembangunan hutan tanaman sengon dapat dipengaruhi oleh beberapa faktor pembatas pertumbuhan, salah satunya adalah ketahanan terhadap serangan hama dan penyakit tanaman. Jenis hama yang paling umum diketahui adalah serangan hama penggerek batang (Xystrocera festiva Thombs.) atau sering dikenal sebagai boktor atau uter-uter. Secara alami tanaman sengon memiliki gen ketahanan terhadap serangan hama boktor, salah satunya yaitu gen Heat shock cognate 70 kDA protein (HSP 70). Penelitian ini bertujuan untuk menguji tingkat ekspresi gen HSP 70 secara kuantitatif terhadap kambium pohon sengon resisten dan sengon terserang hama boktor melalui teknik Real Time PCR. Nilai referensi yang dipilih berupa level ekspresi housekeeping gene menggunakan gen Actin 1 dan gen target menggunakan gen HSP 70, melalui perhitungan ΔCt = Cttarget gene – Cthousekeeping gene. Hasil menunjukkan gen HSP 70 lebih besar terekspresi pada sampel resisten dibandingkan dengan sampel terserang boktor., Sengon (Falcataria moluccana Miq.) is one of the most multipurpose trees in Indonesia. The success of sengon plantation forest development can be influenced by several growth limiting factors, one of these is resistance against pest and disease. The most common type of pest known is stalk borer (Xystrocera festiva Thombs.) or often known as boktor or uter-uter. Naturally, sengon plants have genes for resistance to boktor, one of them is the Heat shock cognate 70kDA Protein (HSP 70) gene. The study aims to test the expression of a gene HSP 70 in a quantitative manner against the cambium trees resistance and sengon attacked by boktor through Real Time PCR technique. The reference level selected housekeeping gene expression in the form of using genes Actin 1 and gene HSP 70 is used, trought calculation ΔCt = Cttarget gene – Cthousekeeping gene. Results showing that genes of HSP 70 greater expression in samples of resistance than infested by boktor.
Keyword: comparative CT method, Falcataria moluccana, HSP 70

Judul: Studying rate of absorption steam by solutions Lithium Bromide (LiBr) as absorbant in absorption refrigeration
Abstrak: Absorption is the process of absorbing a fluid by another fluid to form a fluid solution. In absorption refrigeration system, LiBr as absorbent filters on components of the absorber will absorb water vapor with lower pressure than the evaporator pressure. The rate of absorption of water vapor is a function of concentration, temperature, humidity and pressure of water vapor. The goal of this research is to see the effect of concentration, temperature and vapor pressure of water at the rate of absorption of water vapor by aqueous absorbent filters (LiBr-H2O) in relation to the cooling system, to determine the equilibrium concentration absorption solution of LiBr-H2O, and to determine the sorption isotherm model. Equilibrium concentration is a function of temperature and humidity. Ce at 40oC and RH 60% was found 37%; at 40oC and RH 70% was 34%; at 40o and RH 80% was 29%; at 45oC and RH 60% was 37.5%; at 45oC and RH 70% was 36%; and at 45oC and RH 80% was 34.5%. The calculation results of Qe using Qe sorption isotherm model on temperature 40oC and 45oC were not too different. The calculation results of the percentage errors between Qe data and model from each of the humidity, obtained the lowest percentage of errors in model Freundlich was 0.4% in RH 60% and 70%; 0.3% in RH 80%.
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