Judul: Perancangan roof garden pada atap gedung Botani Square sebagai media introduksi roof garden di Kota Bogor
Abstrak: Pembangunan infrastruktur yang pesat telah menekan jumlah ruang terbuka hijau di Kota Bogor. Jumlah ruang terbuka hijau menurun dari 31% hingga 14% sepanjang periode 1972-2011. Percepatan penambahan ruang terbuka hijau dapat dilakukan dengan menerapkan sistem roof garden pada setiap atap gedung di Kota bogor. Perancangan Roof Garden pada Atap Gedung Botani Square sebagai Media Introduksi Roof Garden di Kota bogor bertujuan untuk menganalisis potensi dan kendala terhadap bagunan gedung Mal Botani Square, serta membuat perancangan model roof garden sebagai media introduksi bagi masyarakat. Metode yang digunakan adalah modifikasi Simons (1983) meliputi survei pendahuluan, pengambilan data, analisis, sintesis, dan perancangan. Analisis biofisik, fisik, dan struktur menghasilkan gambaran potensi dan kendala yang menjadi landasan sintesis dan konsep pada tapak. Konsep Roof Top Cafe dipilih agar menarik minat pengunjung, serta menambahkan peran edukasi sebagai media introduksi roof garden. Pemilihan material serta teknologi instalasi yang ringan mampu menekan total bobot desain roof garden. Bobot merata maksimum pada desain sebesar 258,01 kg/m2 mampu ditopang oleh struktur eksisting. Hasil akhir dari penelitian ini adalah gambar rencana tapak, gambar potongan, gambar detil, serta ilustrasi 3D
Keyword: green open space, design, roof garden

Judul: Desain Roof Top Garden berbasis Landscape Engineering Adjustment di Apartemen The Suites @Metro Kota Bandung
Abstrak: Roof Garden merupakan sebuah inovasi dalam pertamanan dengan memanfaatkan ilmu rekayasa lanskap pada atap bangunan. Hal tersebut menjadi sebuah metode untuk memperluas luasan ruang terbuka hijau (RTH) sebagaimana yang dianjurkan pada UU no. 26/2007 proporsi 30% RTH harus dipenuhi oleh sebuah kota. Kota Bandung salah satu perkotaan padat di Jawa Barat dijadikan sebagai lokasi untuk penelitian ini. Penelitian ini bertujuan menganalisis masalah dan potensi pada tapak roof top garden untuk dijadikan sebuah desain referensi bagi pengembangan RTH di atap bangunan. Penerapan metode proses desain Booth (1999) pada taman atap di gedung Apartemen The Suites @Metro Kota Bandung dengan pendekatan spasial dan perhitungan struktur hijau menggunakan green area ratio serta SAP 2000 sebagai acuan dari rekayasa lanskap. Hasilnya disintesiskan menjadi sebuah konsep dasar desain yaitu “Mandala Atma”. Konsep tersebut bertujuan untuk membagi ruang dan mendapatkan suasana natural di roof top garden yang dipadukan karakter budaya Sunda. Hasil penelitian berupa siteplan desain roof top garden beserta detail pada Apartemen The Suites @Metro Kota Bandung. Hasil ini diharapkan dapat terciptanya roof top garden yang efisien terhadap material, berkelanjutan, dan ekologis., Roof Garden is an innovation of landscaping by utilizing landscape engineering science on the roof of the building. This development has become a method for expanding the area of green open space as recommended in UU no. 26/2007 the proportion of 30% green open space must be met by a city. The city of Bandung, one of the densest cities in West Java, was used as the location for this research. This study aims to analyze the problems and potential of the roof top garden site to be used as a reference design for the development of green open space on the roof of the building. The application of the Booth (1998) design process method on the rooftop garden at The Suites @Metro Apartment in Bandung City with a spatial approach and green structure calculations using the green area ratio and SAP 2000 as a reference for landscape engineering. The results are synthesized into a basic design concept, namely "Mandala Atma". The concept aims to divide space and get a natural atmosphere in the roof top garden combined with the traditional character of Sundanese culture. The results of the research are in the form of a site plan design along with details of the roof garden at The Suites @Metro Apartment, Bandung City The result is to create material efficient, sustainable, and ecological roof gardens.
Keyword: Cities, Green Infrastructure, Green Roof, Structure, Urban Heat Island

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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