Judul: Studi banding kesesuaian disain ventilasi pada tutup kotak lebah terhadap perkembangan koloni
Abstrak: Sejak lama kehidupan lebah madu diyakini telah menun- jang kebutuhan hidup manusia. Sejak diperkenalkannya lebah madu Apis mellifera di Indonesia, tampak telah memacu masyarakat untuk membudidayakan lebah madu dengan teknik yang lebih modern daripada teknik perlebahan sebe- lumnya. Salah satu komponen budidaya lebah madu secara modern adalah digunakannya kotak pemeliharaan yang memberi kemudahan dalam pengelolaan. Penggunaan kotak pemeliharaan lebah adalah untuk kemudahan pengelolaan koloni dan untuk mendapatkan hasil yang lebih baik. Kelengkapan dan kesempurnaan kotak lebah mempunyai andil yang cukup besar dalam keberhasilan budidaya lebah madu. Tingkat kenyamanan disain kotak terutama dinyatakan pada kondisi mikroklimat di dalam kotak. Kondisi mikroklimat yang sesuai dengan syarat kehidupan telur, larva dan pupa memberi dukungan yang baik dalam proses perkembangan dan pertumbuhan yang sempurna....
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Judul: Pengaruh Pemberian Ransum Makanan Buatan terhadap Pertumbuhan dan Perkembangan Koloni Lebah Madu Apis mellifera Linn
Abstrak: Lebah madu merupakan salah satu serangga yang banyak hidup di hutan dan menghasilkan hasil hutan non kayu yang berupa antara lain madu dan lilin lebah. Salah satu syarat untuk pengembangan budidaya lebah madu adalah tersedianya sumber pakan lebah (*bee forage*) yang cukup dan berkesinambungan. Dalam hal ini pengembangan koloni lebah madu memerlukan ketersediaan serbuk sari bunga/polen dan nektar yang memadai dari beraneka ragam bunga jenis tanaman pakan lebah.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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