Judul: Flushing pada Tanaman Amherstia nobilis dan Brownea capitella di Kebun Raya Bogor
Abstrak: Phenology is a study on periodicity sightings of plants in relation to climate, such as flowering time and flushing. Flushing means the growth of shoots/ flush simultaneously. This study aims to observe the shoots and leaves growth in response to light intensity under the difference of growing positions and environmental factors. This study used purposive sampling as a descriptive method. The growing positions of flush were on terminal and axillary of the plant, both in exposed and shaded conditions. The growth pace speeds of Amherstia nobilis and Brownea capitella in exposed condition were faster than shaded condition. The rate of growth was accompanied by a change of color. The changing color of A. nobilis leaves were faster for two months than B. capitella. This study showed that light intensity and growing positions of the plants (terminal and axillary) influenced the growth of both species.
Keyword: Phenology, Flushing, Flush, Amherstia nobilis, Brownea capitella

Judul: Studi fenologi pembungaan dan flushing fabaceae
Abstrak: Penelitian ini dilakukan untuk mengetahui masa pembungaan/flushing pada berbagai spesies Fabaceae, panjang masa berbunganya dan unsur iklim yang mempengaruhinya. Penelitian ini dilaksanakan di dalam Kampus IPB Darmaga, Bogor mulai dari Desember 2004 hingga Desember 2005. Penelitian menggunakan metode deskriptif. Jumlah spesies yang digunakan sepuluh yaitu: Bauhinia purpuera, Cassia glauca, Delonix regia, Erythrina crista-galii, Peltophorum sp., Pterocarpus indicus, Amherstia nobilis, Brownea sp., Maniltoa grandiflora dan Saracca thaipingensis. Sampel yang diamati adalah 30 dengan tiga ulangan kecuali D. regia empat ulangan dan S. thaipingensis dua ulangan karena keterbatasan sampel.
Keyword: 

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
Keyword: