Judul: Sifat-sifat dan klasifikasi beberapa tanah latosol daerah Bogor - Jakarta
Abstrak: Tujuan penelitian ini adalah untuk mengetahui sifat sifat dan mengklasifikasikan beberapa tanah Latosol di daerah Bogor Jakarta menurut sistem klasifikasi Taksonomi Tanah. Untuk mencapai tujuan ini, maka dilakukan penelitian di lapang dan laboratorium terhadap tiga profil tanah di daerah Bogor - Jakarta. Berdasarkan data lapang, iklim, dan data hasil analisa laboratorium, maka ketiga macam tanah yang diteliti ditentukan sifat-sifat tanahnya, dan diklasifikasikan sampai kategori family berdasarkan kriteria Taksonomi Tanah. Latosol Coklat dari Desa Tajurhalang, Bogor, mempunyai cadangan mineral (kesuburan alami) sangat tinggi, sifat fisik baik, kesuburan tanahnya sedang. Tanah ini pada kategori family diklasifikasikan sebagai "Typic Dystro pept, very fine clayey, halloysitic, isohyperthermic". Latosol Coklat Kemerahan dari Desa Darmaga, Bogor, mempunyai cadangan mineral tinggi, sifat fisik baik, kesuburan tanahnya rendah sampai sedang. Pada kategori family tanah ini diklasifikasikan sebagai "Oxic Dystropept, very fine clayey, kaolinitic, isohyperthermic".
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Judul: Sifat-sifat dan Klasifikasi Beberapa Jenis Tanah dari Desa Sukajaya, Ciomas dan Sekitarnya Menurut Sistem Taksonomi Tanah
Abstrak: Penelitian ini bertujuan untuk mempelajari sifat-sifat tanah Andosol Coklat, Latosol Coklat, dan Latosol Coklat Kemerahan dari kecamatan Ciomas, Bogor dan mengklasifikasikannya menurut sistem Taksonomi Tanah sampai kategori family (keluarga). Penelitian ini dilakukan dengan metoda pengamatan di lapang dan analisa contoh tanah di laboratorium. Hasil penelitian menunjukkan bahwa ketiga jenis tanah yang diteliti berasal dari bahan vilkanik andesitik dengan esosiasi hiperten sampai hiperten-augit untuk tanah Latosol Coklat Kemerahan, serta asosiaso hiperten-augit untuk tanah Andosol Coklat.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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