Judul: Evaluasi Potensi Lanskap Kampung Batik Laweyan, Surakarta sebagai Wisata Budaya.
Abstrak: Batik telah ditekuni masyarakat di Pulau Jawa, salah satunya masyarakat Kampung Laweyan. Sejak 2004, Kampung Laweyan telah ditetapkan sebagai destinasi wisata budaya, dan terus mengalami peningkatan intensitas wisata. Daya tarik yang dimiliki Kampung Batik Laweyan berupa kekayaan budaya baik budaya tangible (bendawi) maupun intangible (non bendawi). Berbagai potensi tersebut belum dikembangkan dan dikelola secara optimal sehingga memunculkan permasalahan yang dapat mengancam keberlanjutan Kampung Batik Laweyan sebagai wisata budaya. Oleh karena itu, diperlukan evaluasi potensi wisata budaya. Lokasi penelitian berada di Kampung Laweyan, Surakarta dengan luas 24,83 Ha. Penelitian ini berlangsung dari bulan Januari hingga September 2018. Metode yang digunakan adalah analisis potensi objek dan atraksi wisata budaya berdasarkan kriteria signifikansi budaya berdasarkan Burra Charter (ICOMOS 1999) dan Avenzora (2008), analisis Penilaian Keberlanjutan Masyarakat (PKM), serta analisis persepsi dan preferensi masyarakat dan pengunjung. Tujuan penelitian ini adalah menganalisis potensi lanskap Kampung Batik Laweyan. Berdasarkan hasil penilaian potensi wisata budaya, Dusun Setono, Sayangan Kulon, dan Sayangan Wetan termasuk ke dalam kategori sangat potensial, sedangkan Dusun Lor Pasar, Klaseman, Kidul Pasar, Kramat, dan Kwanggan termasuk ke dalam kategori potensial. Hasil PKM terhadap aspek ekologi (153), aspek sosial (318), dan aspek spiritual (300) menghasilkan nilai total skor (771) yang menunjukkan status keberlanjutan mencapai suatu awal yang baik sebagai ecovillage. Adapun hasil analisis persepsi preferensi masyarakat dan pengunjung menunjukkan penerimaan serta tingkat partisipasi masyarakat yang tinggi dalam pengembangan Kampung Laweyan sebagai kampung wisata budaya. Berdasarkan hasil analisis, diusulkan rekomendasi pelestarian kampung serta keberlanjutan masyarakat. Rekomendasi pelestarian berfokus pada pengembangan potensi wisata yang disusun berdasarkan zonasi wisata (zona wisata inti dan zona wisata pengembangan). Rekomendasi keberlanjutan masyarakat berfokus pada pengolahan limbah batik, pengembangan industri batik, pelestarian budaya, dan pensinergisan kepentingan.
Keyword: batik, evaluasi potensi lanskap, Kampung Batik Laweyan, wisata

Judul: Evaluation of the Landscape  Management of the Batik Trusmi Tourism Village in Cirebon Regency as a Sustainable Tourism Object
Abstrak: Batik merupakan salah satu kekayaan budaya yang dimiliki oleh Indonesia. Salah satunya terdapat di perkampungan Batik Trusmi Cirebon, Batik Trusmi Cirebon bermula sejak abad ke-14, seni batik Cirebon terdapat dua keraton yaitu Keraton Kesepuhan dan Keraton Kanoman. Berbagai potensi yang ada belum dikembangkan secara optimal. Diperlukan evaluasi pengelolaan terhadap Kampung Batik Trusmi. Metode yang digunakan adalah analisis potensi objek dan atraksi wisata budaya berdasarkan kriteria signifikansi budaya Burra Charter (ICOMOS 1999) dan Avenzora (2008) bahwa Kampung Batik Trusmi memiliki potensi untuk dikembangkan sebagai kawasan wisata berkelanjutan, analisis Penilaian Keberlanjutan Masyarakat (PKM) dengan hasil Kampung Batik Trusmi menunjukkan suatu awal yang baik ke arah keberlanjutan, serta analisis persepsi dan preferensi masyarakat dan pengunjung dengan hasil fasilitas dan sarana yang ingin dikembangkan di Kampung Batik Trusmi. Tujuan penelitian ini adalah untuk menganalisis dan mengevaluasi potensi wisata budaya Kampung Batik Trusmi. Hasil dari penelitian ini berupa rekomendasi pelestarian dan rekomendasi keberlanjutan masyarakat., Batik is one of the cultural wealth owned by Indonesia. One of them located in the village of Batik Trusmi Cirebon, Batik Trusmi Cirebon originated in the 14th century, Cirebon batik art has two palaces, namely the Kesepuhan Palace and the Kanoman Palace. Various potentials that exist have not been developed optimally. It is necessary to evaluate management of Trusmi Batik Village. The method used is the analysis of the potential of cultural tourism objects and attractions based on the criteria of cultural significance of Burra Charter (ICOMOS 1999) and Avenzora (2008) that Trusmi Batik Village has the potential to be developed as a sustainable tourism area, analysis of Community Sustainability Assessment (PKM) with the results of Kampung Batik Trusmi shows a good start towards sustainability, as well as an analysis of the perceptions and preferences of the community and visitors with the results of the facilities and facilities that want to be developed in Trusmi Batik Village. The purpose of this study was to analyze and evaluate the cultural tourism potential of Trusmi Batik Village. The results of this study are the form of conservation recommendations and community sustainability recommendations.
Keyword: Batik, Batik Village, Trusmi Batik Village, Tourism Village

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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