Judul: Variasi Alel dari Gen 5-HTT (Penyandi Agresivitas) pada Macaca nemestrina
Abstrak: Macaca nemestrina is an aggressive primate. The aggresivity is influenced by many factors, one of them is by a gene. The 5-HTT gene encodes the serotonin transporter that affects the regulation of serotonergic system and its receptor by modulating the concentration of serotonin in extracellular fluid. Variation in promoter and intron region can change the transcriptional activity of the 5-HTT gene, mainly the activity of the transporter and serotonin uptake. The transporter change activity influenced aggressivity, emotion, motor function, and some cognitive traits in primates. This research aimed to determine alelles variant of 5-HTT gene (encoding aggresivity) in M. nemestrina. Samples used in this research consisted of 56 M. nemestrina. The gene was amplified by the Polymerase Chain Reaction (PCR) method. Amplification in promoter region revealed two alleles and three genotypes with the frequencies of L and S alleles were 88,18% and 11,82%, respectively. Amplification of the third intron region showed two alleles with the frequency of l and s alleles were 55,4% and 44,6%, respectively. None of the individual had s/s genotype in the third intron region. Short allele was dominant to long allele. The presence of at least one short allele decreased transcription activity. There was one individual with S/S genotype in promoter region that was possibly to be the alpha male in the group.
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Judul: Keragaman Gen Monoamine  Oxidase A (MAOA) pada Sapi Pedaging
Abstrak: One of the essential traits in the management of raising meat livestock is the aggressive nature of cattle, which affects livestock handling and productivity. Several genes control aggressive traits, and one that plays an important role is the monoamine oxidase A (MAOA) gene. The MAOA gene encodes the enzyme monoamine oxidase A, which plays a role in the catabolism of neurotransmitters, including dopamine, norepinephrine, and serotonin. Mutations in the MAOA gene can increase the tendency of cows to behave aggressively, which can affect neurotransmitter regulation. Studies on MAOA gene diversity in Indonesian beef cattle have yet to be carried out. Therefore, this research was conducted to identify and analyze SNP determination and MAOA gene diversity in beef cattle in Indonesia. One hundred twenty-seven blood samples from beef cattle were from Bali, Limousin, Wagyu, PO, Madura, and Balinese Wagyu crosses. Blood samples were extracted using the GeneAid kit, isolating pure DNA from beef cattle. MAOA gene diversity was analyzed using the PCR-RFLP method. Analysis results such as genotype frequencies, allele frequencies, heterozygosity values, and Hardy-Weinberg balance were calculated using Popgen32. Amplification of the MAOA gene sequence in the promoter fragment and exon 1 via PCR was successfully carried out at 57 ºC, resulting in product lengths of 548 bp and 341 bp, respectively. The results showed that the MAOA gene located in the promoter region has six SNPs that are specific to Bali cattle, namely SNP g.98 A > C, SNP g.145 G > T, SNP g.216 A > C, SNP g.340 T > G, SNP g.349 A > C, and SNP g.385 G > A. Meanwhile, the MAOA gene located in exon 1 is monomorphic. The PCR-RFLP method was used to investigate the SNP g.385 G > A MAOA gene polymorphism using the RSaI restriction enzyme. The MAOA SNP g.385 G > A gene obtained two alleles (G and A) and three genotypes: GG, GA, and AA. SNP g.385 G > A is polymorphic in Bali, PO, Madura, and Wagyu-Bali cross (F1) cattle while monomorphic in Limousin and Wagyu cattle. The allele frequency of the SNP g.385 G > A MAOA gene in Bali, PO, Madurese cattle, and Wagyu-Bali crosses (F1) is in Hardy-Weinberg equilibrium. SNP g.385 G > A of the MAOA gene can be used as a candidate aggressive genetic marker., Salah satu sifat yang penting dalam manajemen pemeliharaan ternak daging adalah sifat agresif pada sapi yang berpengaruh pada penanganan ternak dan produktivitas. Sifat agresif dikendalikan oleh beberapa gen dan salah satu yang berperan penting adalah gen MAOA. Gen MAOA mengkodekan enzim monoamine oksidase A yang berperan dalam katabolisme neurotransmiter, termasuk dopamin, norepinefrin, dan serotonin. Mutasi pada gen MAOA dapat meningkatkan kecenderungan sapi untuk berperilaku agresif yang dapat mempengaruhi regulasi neurotransmitter. Studi keragaman gen MAOA pada sapi pedaging Indonesia belum dilakukan. Oleh karena itu, penelitian ini dilakukan untuk mengidentifikasi dan menganalisis penetuan SNP dan keragaman gen MAOA pada sapi pedaging di Indonesia. Sebanyak 127 sampel darah sapi pedaging yang terdiri dari sampel darah sapi Bali, Limousin, Wagyu, PO, Madura, dan silangan Wagyu Bali. Sampel darah diekstraksi menggunakan kit GeneAid dan
Keyword: beef cattle, MAOA gene, SNP

Judul: Perencanaan Jaringan Irigasi Curah Otomatis Untuk Tanaman Jagung di Kebun Pendidikan Cikabayan
Abstrak: Indonesia memiliki potensi sumber daya air yang dapat dimanfaatkan untuk mendukung sektor pertanian dalam memenuhi kebutuhan irigasi. Irigasi pada Kebun Percobaan Cikabayan masih berupa irigasi konvensional. Penerapan irigasi curah diperlukan untuk menghindari kekurangan air tanaman pada saat musim kemarau yang dapat mengubah sistem pengairan yang terlalu memboroskan air menjadi sistem pengairan yang efisien dan efektif. Oleh karena itu, tujuan penelitian ini adalah merancang jaringan irigasi curah otomatis tanaman jagung di Kebun Pendidikan Cikabayan. Kebutuhan air irigasi diperoleh 5,4 mm/hari dengan waktu penyiraman perhari 3 jam 37 menit. Pencurah yang digunakan tipe sprinkler rotor dengan diameter nozel 2 mm, tekanan operasi 20 m, radius curahan 11 m, dan debit pencurah 0,06 liter/detik. Pipa utama skema 1 memiliki diameter 2 inch, pipa subutama 2 inch, pipa manifold 1 inch, dan pipa lateral 0,75 inch. Pipa utama skema 2 memiliki diameter 1,5 inch, pipa subutama 1,5 inch, pipa manifold 1 inch, dan pipa lateral 0,75 inch. Pompa yang digunakan sentrifugal, dimana skema 1 memiliki tekanan 28,50 m dengan debit 1,71 liter/detik dan skema 2 memiliki tekanan 29,99 m dengan debit 0,88 liter/detik. Estimasi rencana anggaran biaya konstruksi untuk skema 1 Rp54.950.000,00 dan skema 2 sebesar Rp43.800.000,00.
Keyword: corn, otomatic, sprinkler, sprinkler irrigation