Judul: Identifikasi keragaman gen penyandi tahan panas (Heat Shock Protein 70) ayam lokal serta respon fisiologisnya terhadap cekaman panas akut
Abstrak: This study was designed to obtain HSP 70 genotypes of chickens which were tolerant to high ambient temperature or heat stress, while the specific objectives were: 1) To examine the HSP 70 gene polymorphism in local, Arabic, and commercial chickens, 2) To examine the genotype effect of local, arabic, and commercial chickens on their adaptability to the high environmental temperature. The study used 96 blood samples from local chicken, 94 samples from Arabic chickens, and 87 samples from commercial chickens which were taken from the brachial vein at the age of 12 weeks. Day old Kampong chickens were obtained by hatching the eggs that were collected from lowlands, medium, and high altitude areas in Lombok Island, while the day old Arabic chickens were obtained from local farmer. Day old commercial chickens were purchased from the local poultry shop. Each genotype from each chicken classes wasrepresented by 4 chickens to be challenged to heat stress at 40oC, one chicken as a control (was not exposed to given heat to heat stress), one chicken was exposed to heat stress for 0.5, 1, and 1.5 hours, respectively. The genotyping results by using PCR-SSCP analysis showed that kampong and Arabic chickens were polymorphic, while commercial chickens were classified as monomorphic. Kampong chickens had seven genotypes of HSP 70, namely AA, AB, AC, CC, AD, DD, and BC. The AD genotypes and A allele had the highest frequency in kampong chickens. Arabic chickens had six genotypes, namely AA, AB, AC, CC, AD, and BC, which AC genotype and A allele had the highest frequencies. However, the commercial chickens had only one genotype, namely DD. Seven were polymorphic sites found in applicated area, namely AG mutation at site 617, AG mutation at sites 628 and G C mutation at site 646, mutation CT at sites 661 and AG mutation at site 699, mutation GC at site 754, and AG mutation at site 792. Mutation at site 628, 646 and 661 were considered as silent mutation. HSP 70 gene in kampong and arabic chickens population in this study were in equilibrium. The values of Ho, He, and PIC of kampong and Arabic chickens were not significant. The chicken breeds (local, arabic and commercial chickens) affected the panting frequency, feces water content, and the blood hematocrit values as well, but did not affect the onset of panting, drinking water consumption, feed consumption, rectal temperature, HSP 70 expression, the serum corticosterone concentration, erythrocytes concentration, hemoglobin, leucocyte concentration, heterophils, basophils, lymphocytes, monocytes, eosinophil, and the H/L ratio. Acute heat stress caused an increase in the panting frequency, drinking water consumption, rectal temperature, feces water content, the expression of HSP 70, the serum corticosterone concentration, the heterophils, basophils, and the H/L ratio, and decreased feed intake, erythrocytes, hemoglobin, hematocrit value, leucocyte, lymphocytes, and monocytes percentages. Heat stress treatment did not affect the percentage of eosinophils. Long-term heat stress did not affect the onset of panting, drinking water consumption, feed consumption, rectal temperature, feces water content, serum corticosterone concentration, erythrocytes concentration, hemoglobin, hematocrit values, leucocyte concentration, and heterophils, eosinophils, basophils, lymphocytes, monocytes percentage. However,
Keyword: HSP 70 gene, local chickens, corticosterone, panting, hematologic

Judul: Identifikasi Keragaman SNP g.1117G>A Gen HSP70 pada Sapi Pedaging menggunakan Teknik PCR-RFLP
Abstrak: Indonesia memiliki sumber daya genetik ternak (SDGT) yang melimpah, termasuk ternak sapi. Sapi lokal mempunyai potensi produksi yang baik pada kondisi lingkungan yang minimal dan mampu memanfaatkan pakan berkualitas rendah serta mempunyai daya reproduksi yang baik. Daerah tropis sering mengalami perubahan suhu di atas zona nyaman yang mengakibatkan tenak mengalami stres dan dapat berdampak pada produksi dan reproduksi. Salah satu kandidat gen yang mempengaruhi toleransi terhadap heat stres adalah gen Heat Shock Protein 70 (HSP70). Penelitian ini bertujuan untuk mengidentifikasi polimorfisme SNP g.1117G>A gen HSP70 (HSP70|BanI) menggunakan metode PCR-RFLP. Penelitian ini menggunakan 65 sampel DNA 4 bangsa sapi yang terdiri dari 20 ekor sapi PO, 20 ekor sapi Madura, 12 ekor sapi kuantan dan 13 ekor sapi pesisir yang telah dikoleksi oleh Laboratorium Genetika Molekuler Ternak Fakultas Peternakan IPB. Analisa keragaman gen HSP70 dengan metode PCR-RFLP. Enzim yang digunakan untuk pemotongan adalah BanI primer forward 5'-GAAAGGAAAAAAGAGACAGA-3' dan primer reverse 5'-AATACGCAGGAGTAGGTGGT-3'. Keragaman genetik dari gen HSP70 dianalisis menggunakan frekuensi alel, frekuensi genotipe, derajat heterozigositas. Keseimbangan Hardy-Weinberg diuji dengan uji Chi Kuadrat menggunakan aplikasi PopGen 32. Hasil penelitian menunjukkan bahwa Amplifikasi gen HSP70 terletak di 5’UTR dengan suhu annealing 61 ⁰ C selama 15 detik dengan panjang produk 539 pb. Keragaman gen HSP70 pada bangsa sapi pedaging lokal bersifat polimorfik dengan frekuensi alel G sebesar (0,565) lebih tinggi dibanding frekuensi alel A (0,435)., Indonesia has abundant livestock genetic resources, including cattle. Local cattle have good production potential in minimal environmental conditions and are able to utilize low-quality feed and have good reproductive. The tropics often experience changes in temperature above the comfort zone which causes livestock to experience stress and will have an impact on production and reproduction. One candidate gene that affects tolerance to heat stress is the Heat Shock Protein 70 (HSP70) gene. This study aims to identify the SNP g.1117G>A HSP70 gene polymorphism (HSP70|BanI) using the PCR-RFLP method. This study used 65 DNA samples from 4 breeds of cattle consisting of 20 heads of PO cattle, 20 heads of Madura cattle, 12 heads of Kuantan cattle and 13 heads of Pesisir cattle that have been collected by the Laboratory of Animal Molecular Genetics, Faculty of Animal Science IPB. Analysis of the HSP70 gene diversity was done by using the PCR- RFLP method. The enzyme used for cutting was BanI with forward primer 5'-GAAAGGAAAAAAGAGACAGA-3 'and reverse primers 5'-AATACGCAGGAGTAGGTGGT-3'. The variation of the HSP gene was analyzed using allele frequency, genotype frequency, and degree of heterozygosity. Hardy-Weinberg equilibrium was tested by Chi-Square test using PopGen 32 application. The results showed that the HSP70 gene amplification was located at 5’UTR with an annealing temperature of 61 ⁰ C for 15 seconds with a product length of 539 bp. The HSP70 gene in local beef cattle was polymorphic with the allele frequency G (0,565) higher than the A allele frequency (0,435).
Keyword: HSP70, genetic polymorphism, PCR-RFLP, local beef cattle

Judul: Penyelesaian Masalah Pelabelan Graf vertex Magic pada Graf Cycle Sederhana
Abstrak: Graf vertex magic adalah suatu graf memiliki nilai bilangan magic yang sama pada masing-masing vertex yang berbeda. Graf cycle sederhana adalah suatu walk tertutup yang mengandung setidaknya tiga vertex, semua vertex-nya berbeda dan setiap vertex terhubungkan oleh satu edge.
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