Judul: Characteristics and Quality of Bali Cattle Cement and Bali - Banteng Crossing at Balai Besar Inseminasi Buatan Singosari.
Abstrak: Bali cattle are Indonesian native cattle that have been domesticated from Banteng (Bos Banteng). Bali cattle have a better reproductive ability in the tropics compared to exotic cattle such as Simmental cattle and Limousine cattle. This study aimed to analyze the characteristics and quality of cement of Bali cattle and Jaliteng cattle (Banteng Cross). This study used secondary data from Balai Besar Inseminasi Buatan Singosari, Malang, East Java with a total of 1170 data consisting of 1 Jaliteng cattle with 27 ejaculations, and 33 Bali cattle with 9-47 ejaculations (an average of 35 ejaculations) for one year. Variable data for each breed of cattle were analyzed using the mean, standard deviation, and t-test. Based on the results of the study, it was found that Bali cattle and Jaliteng cattle at BBIB Singosari had significantly different characteristics and cement quality and that crossing between Bali and Banteng cattle has an impact on the decline in sperm in Bali cattle. The results of this study showed that the cement of Bali cattle and Jaliteng cattle had good characteristics and quality and met the eligibility standards to be processed and distributed either into liquid cement or frozen cement for artificial insemination programs.
Keyword: ali Cattle, Jaliteng Cattle, Quality of Sperm

Judul: Female Reproductive Biology and Application of Artificial Insemination in Banteng (Bos javanicus d’Alton 1823)
Abstrak: The banteng (Bos javanicus d'Alton 1823) is one of the original Indonesian germplasms to be developed and conserved, and is considered endangered wildlife according to the IUCN Red List (IUCN 2010). The population of wild banteng has declined in many countries. The population decline is caused by illegal hunting, habitat destruction, and the spread of domestic animal-derived diseases. To increase the banteng population in the wild, various efforts are needed, including the use of artificial insemination (AI) reproductive technology. The breeding success of an animal species depends on several interrelated factors, one of which is the reproductive organs factor. Information on the reproductive biology of the Banteng as an ancestor of the Bali cattle is still very limited and poorly known, and knowledge of mating management in the Banteng is still lacking. Basic knowledge of ovarian characteristics related to the estrous cycle and monitoring methods are therefore very important for the success of a mating process. This research was extensively conducted between the monitoring of the reproductive cycle and the changes in the condition of the reproductive organs, namely by monitoring the reproductive cycle using ultrasound (USG). This study consists of 3 experiments. The first experiment aims to describe the characteristics of vaginal cytology and during estrus cycle and observe the clinical signs of estrus Banteng. Two healthy, sexually mature and fertile Banteng females from Taman Safari Indonesia were used for this study. Observation of clinical estrus symptoms and vaginal swabs were performed three times a week and for 5 consecutive days before and after estrus. The obtained vaginal swabs were then stained with Giemsa 0.24%. The epithelial cells obtained were parabasal, intermediate, superficial, and keratinized. Superficial cells are very dominant compared to other cells when estrus occurs, which corresponds to 50.20%, followed by keratinized cells with 38.51%, while other epithelial cells decreased, namely intermediate cells 26.82% and parabasal cells 13.44% for 3 estrus cycles. Readiness to be mounted by a bull or other female banteng at the time of oestrus is the most obvious clinical sign, and vaginal cytology procedures can be used to determine the oestrus cycle of banteng. The second experiment aimed to investigate the use of artificial insemination (AI) techniques based on the results of ultrasonic examination of the reproductive organs of the Banteng. Use of 2 female banteng in Taman Safari Indonesia, Cisarua, Bogor. Habituation to facilitate data collection. Optimal timing for reproduction is determined by examining the reproductive organs using ultrasound (USG) to detect changes in the uterus and ovaries and monitor clinical signs. Examination of follicle size and changes in the uterus of each banteng was performed on days -1, 0, and +1 for 3 oestrus cycles. Insemination is performed 2- 3 times during oestrus until ovulation occurs. The behavior of the banteng is easy to handle, they feel safe, calm and comfortable in the service box. The mean follicle size of the two Banteng females Andini and Uchi was 1.09 ± 0.02 cm on day -1, 1.31 ± 0.02 cm on day 0, and 1.33 ± 0.03 cm on day +1. Artificial insemination was
Keyword: banteng, vaginal cytology, artificial insemination, follicle size

Judul: Electrochemical Sensor Based on Graphene Oxide/PEDOT:PSS Decorated with AuAg Nanozyme for H2O2 Detection
Abstrak: H2O2 merupakan senyawa penanda hayati keadaan stres oksidatif tubuh sehingga diperlukan metode deteksi H2O2 yang cepat dan akurat. Sensor elektrokimia berbasis nanozyme peroksidase, seperti Au-NPs dan Ag-NPs, menawarkan alternatif metode deteksi yang menjanjikan karena ukuran alat yang kecil, kinerja analitik yang lebih baik, dan efisien biaya. Penelitian ini bertujuan mengembangkan sensor elektrokimia non-enzimatik menggunakan nanopartikel bimetal AuAg pada elektrode karbon kaca yang dimodifikasi dengan komposit grafena oksida dan poli(3,4-etilenadioksitiofena)-poli(stirena-4-sulfonat). Elektrode termodifikasi bimetal AuAg-NPs menunjukkan respons pada H2O2 yang lebih tinggi daripada elektrode termodifikasi monologamnya, dengan respons linear dalam rentang konsentrasi H2O2 75–200 µM, sensitivitas 0,0407 μA μM-1, dan limit deteksi mencapai 25 μM. Elektrode hasil modifikasi menunjukkan keterulangan pengukuran yang baik dan selektif terhadap H2O2. Elektrode yang dimodifikasi berhasil digunakan untuk mengukur kadar H2O2 dalam sampel urine sintetis serta memberikan hasil yang tidak berbeda nyata dengan metode spektrofotometri.
Keyword: AuAg-NPs, electrochemical sensor, graphene oxide, H2O2, PEDOT:PSS