Judul: In Vitro Shoot Induction of Kaempferia parviflora Wall. Ex. Baker
Abstrak: This research aimed to find the best MS salt concentration and cytokinin (BAP) concentration for K. parviflora rapid multiplication by using in vitro buds as explants. The experiment was done at Plant Tissue Culture Laboratory of Agronomy and Horticulture Department, Faculty of Agriculture, Bogor Agricultural University from January to November 2011. This research began by sterilizing shoots of K. parviflora rhizomes received from PT. Ogawa Indonesia to produce axenic culture. After about 8 weeks of growth, young shoots began to sprout. The young shoots of K. parviflora axenic culture were then cut and cultured individually on the treatment medium, which consist of two different MS salt concentrations (MS and ½ MS) and five different 6-benzylaminopurine (BAP) concentrations (0, 1.25, 2.5, 3.75, and 5 ppm) respectively. Explants started to grow after 2 weeks of culture. Both MS salt concentration and BAP concentration treatments did not give a significant effect on the first shoot induction time, but ½ MS medium supplemented with 5 ppm BAP could induce first shoot faster than other treatments, which is within an average of 2.5 weeks after cultured (WAC). At early observation (2 to 4 WAC), medium enriched with 3.75 ppm BAP induced more shoot than the other treatments, but at the end, control medium (MS0) induced more shoot than other treatments. Starting from 2 WAC, the explants cultured on 0 ppm BAP medium grow more rapidly than the other treatments, but after 10 WAC, they grow slower. All treatments have the same trend of leaf growth. At 2 to 8 WAC, the number of leaves is rising rapidly, but at the 9 WAC, it began to slow down.
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Judul: Pematahan Dormansi Rimpang Kaempferia parviflora Wall Ex. Baker
Abstrak: Kaempferia parviflora (Zingiberaceae family) has a potency to be developed for a new medicinal plants in Indonesia. One of problems in its cultivation is the lack of planting material availability due to the long dormant period. The objectives of this research were to study the effect of various concentrations of BAP and ethephon, and also the effect of the prior growing condition to the dormancy breaking and shoot growth of K. parviflora rhizome. Two separate experiments were applied for two lots of rhizomes namely Cikabayan and Pasir Sarongge lots. Each experiment was arranged in completely random design with 2 factors. Experiment-1 used 4 concentrations of BAP (0, 50, 100, and 150 ppm) as the first factor. While, experiment-2 used 5 concentrations of ethephon (0, 200, 400, 600 and 800 ppm) as the first factor. The second factor in both experiments were prior shade condition at 55% artificial shading and natural shading. A rhizome without soaked application (control) and a rhizome with soaked by aquadestilata without PGRs (0 ppm) were used as compared items. Data was analyzed with ANOVA. Combined Analysis of Variance and t-test were performed in order to compare between 2 lots. Result showed that concentration of BAP until 150 ppm and concentration of ethephon until 800 ppm were not effective to stimulate bud induction time. Meanwhile, rhizome from 55% artificial shading had a shorter periode of dormancy than the natural shading. In the addition, plants from Pasir Sarongge rhizomes lot had more plant number, plant height, leaf number, leaf length, and leaf width than Cikabayan rhizomes lot.
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Judul: Invers Drazin dari Matriks Toeplitz Singular
Abstrak: Matriks Toeplitz adalah matriks yang setiap unsur pada diagonal utama bernilai sama dan setiap unsur pada subdiagonal yang bersesuaian dengan diagonal utama juga bernilai sama. Matriks singular adalah matriks yang tidak bisa ditentukan inversnya. Akan tetapi, dapat diupayakan untuk ditentukan inversnya menggunakan invers matriks yang diperumum. Invers Drazin merupakan salah satu jenis invers matriks yang diperumum. Pada karya ilmiah ini ditentukan invers Drazin dari matriks Toeplitz singular dengan menggunakan metode Leverrier Faddeev. Kemudian, ditentukan invers Drazin dari matriks Toeplitz singular yang direpresentasikan sebagai penjumlahan segitiga bawah dan segitiga atas matriks Toeplitz berdasar pada teorema Gohberg dan Semencul., Toeplitz matrix is a matrix in with element on the main diagonal has the same value and on the subdiagonal corresponding to the main diagonal also has the same value. Singular matrix is a matrix that has no inverse. However, it can be attempted to determine the inverse using a generalized inverse matrix. Inverse Drazin is a type of generalized matrix inverse. This paper discussed the inverse Drazin for a singular Toeplitz matrix using the Leverrier Faddeev method. Then, the Drazin inverse of the singular Toeplitz matrix which is represented as a sum of products of lower and upper triangular Toeplitz matrix based on Gohberg and Semencul theorem.
Keyword: inverse Drazine, singular Toeplitz matrix