Judul: Pengembangan Pertanian Organik di Kelompok Tani Madya Desa Kebonagung, Kabupaten Bantul, Daerah Istimewa Yogyakarta
Abstrak: Organic farming is agricultural activities that seek the use of outside intake and avoid the use of pesticides and synthetic fertilizer. The results showed that the organic farmers and conventional farmers have the high responses of organic farming. However, the characteristics of organic farmer have no relation with farmer’s respons of organic farming. Then, in conventional farmers, formal education and the courage to take the risks have relation with farmer’s respons of organic farming. This research also showed there are no relation between farmer’s respons of organic farming with farmer’s income. Nevertheless, can be predicted that there are chances of the conventional farmers to adopt the organic farming
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Judul: Hubungan Partisipasi Petani Dalam Pertanian Organik Dengan Taraf Hidup (Kasus: Kelurahan Beji, Kecamatan Nguntoronadi, Kabupaten Wonogiri)
Abstrak: Pengembangan sektor ekonomi domestik diharapkan mampu meningkatkan kedaulatan pangan di Indonesia. Salah satu sasaran dari peningkatan kedaulatan pangan yaitu pengembangan 1000 Desa Pertanian Organik. Pengembangan 1000 Desa Pertanian Organik tidak akan berjalan dengan baik jika tidak adanya partisipasi petani yang beralih ke sistem pertanian organik. Tujuan dari penulisan ini adalah mengidentifikasi hubungan partisipasi dengan taraf hidup setelah mengikuti kegiatan pertanian organik. Metode penelitian yang digunakan untuk menggali fakta, data, dan informasi dalam penelitian ini adalah pendekatan kuantitatif yang didukung oleh data kualitatif. Hasil penelitian ini menemukan bahwa petani berpartisipasi dalam pertanian organik. Namun dari segi taraf hidup setelah berpartisipasi dalam pertanian organik belum menunjukkan peningkatan taraf hidup yang signifikan dari hasil pertanian organik.
Keyword: tingkatan partisipasi, pertanian organik, taraf hidup

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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