Judul: Identifikasi dan Studi Patomorfologi Avian Coronavirus pada Ayam dan Beberapa Spesies Psittacine.\
Abstrak: Birds has an important role in daily lives of Indonesia. Poultry in Indonesia is important as the main source of protein and companion birds are bred to fulfill market demand for pets. Infectious bronchitis virus (IBV) is one of avian coronaviruses that can cause economic loss in poultry and found in various birds. The finding of IBV in birds other than poultry suggests a link between poultry and other birds, but there is limited information on IBV in other birds in Indonesia to assess the risk. Indonesia has several endemic Psittacine species often bred to be kept as pet to help in conservation effort. Research of viral diseases which affect productivity such as IBV in these species is very limited, despite its importance for conservation and disease prevention. Meanwhile in poultry, IBV prevention in Indonesia still faces trouble such as difficulty for IBV detection, ineffective vaccination, and lack of information of pathological lesion in field cases. Sufficient information from both sectors is required to assess the link between poultry and companion birds. Thus, this research aimed to find better detection method of IBV cases and its pathomorphological lesion in chicken as well as identifying avian coronavirus in several Psittacine species commonly bred for companion birds. For poultry research, a total of 11 flock samples of suspected IBV infections were collected from cases submitted to Pathology Division, Faculty of Veterinary Medicine, IPB University, and from farms around Bogor, Sukabumi, and Padang. Organ samples were analyzed by RT PCR using two primer sets: IBVN+/IBVN- targeting partial gene N and XCE+/XCE- targeting partial gene S of IBV. RT PCR result showed one sample is positive for gene S, 5 samples are positives for gene N. All positive samples of N gene were proven positive by immunohistochemistry, showing that RT PCR targeting N gene had better sensitivity than S1 gene. Due to mutations often occurring within S1 gene, detection by RT PCR targeting S1 gene can be challenging despite pathological lesion showing IBV infection. The mutation may caused primers unable to capture new field strains, causing false negatives and requires researchers to design new primers. However, S1 gene is still important because it plays a role in immunoprotectivity, which is important for vaccination, and thus still need to be identified after N gene positive result is found. Immunohistochemistry by Ostrich IgY anti-IB Antibody showed the virus could be found on the trachea, kidney, and oviduct of positive samples which may differ in the degree of severity and or microscopic lesion depending on the phase of infection and the strain of infecting virus. For psittacine research, cloacal swabs were taken from 41 healthy Psitaccine birds from two breeding facilities consisted of 15 sulfur-crested cockatoos (Cacatua sulphurea), 10 palm cockatoos (Probosciger aterrimus), 10 Eclectus parrot (Eclectus roratus), two blue and yellow macaws (Ara ararauna), two green winged macaws (Ara chloropterus), and two scarlet macaws (Ara macao). One batch of swab samples were inoculated to Specific Pathogen Free (SPF) embryonated egg for allantoic fluid and embryo. Swab samples and allantoic fluid samples were tested by RT PCR for avian
Keyword: Avian Coronavirus, Immunohistochemistry, Psittacine, Infectious Bronchitis, RT PCR

Judul: Kajian Keberadaan Virus Avian Influenza pada Unggas di Pasar Burung Pramuka, Jakarta
Abstrak: Avian Influenza (AI) which is caused by influenzavirus A of the family Orthomyxoviridae cause an important impact, including in health, social and economic sectors. AI viruses (AIV) can infect various birds and mammals, therefore bird market as a meeting place for human and poultry can potentially transmit AI virus among birds or from birds to human. Indonesia is a habitat of various bird species; therefore research about AIV on bird, especially which are sold in the Pramuka Bird Market, as the biggest bird market in Jakarta, is urgently needed. In general, the aim of this study was to examine the presence of AIV in various bird species sold in Pramuka Bird Market, Jakarta. Specifically, the aims of this study were: (1) to assess the presence of H5 AI virus in oropharyngeal swab, cloacal swab, and fecal samples of various bird species using real time Reverse Transcriptase-Polymerase Chain Reaction (rRT-PCR); (2) to detect the presence of antibodies against AIV (H5) in various bird species using hemagglutination inhibition (HI) test, and (3) to determine the relationship among weather and geographic origin of birds to the prevalence of AIV. During April until September 2011, a number of 862 field samples from 28 families and 96 bird species were collected in Pramuka Bird Market, Jakarta. These field samples consisted of 649 fecal samples, 114 cloacal swab samples, and 99 oropharyngeal swab samples. As many as 7 of 649 faecal samples (1.08%) were positive AI. However, none cloacal or oropharyngel swab were positive AI. Positive fecal samples were detected in four bird species, which are three magpie-robins (Copsychus saularis, family Turdidae), two white-eyes (Zosterops palpebrosus, family Zostropidae), a yellow-vented bulbul (Pycnonotus goiavier, family Pycnotidae), and a robin (Leiothrix lutea, family Sylviidae). In addition to field samples, during April until September 2011, 178 birds from 21 families and 47 species were purchased from the same bird market. From 178 birds, two birds (1.12%), which were two white-eyes (Zosterops palpebrosus, family Zostropidae), were positive AI. The whole positive samples were from Passeriformis order. However, based on rRT-PCR result, there were no H5 positive samples. A number of 163 serum samples were collected from 178 purchased birds from Pramuka Bird Market. Based on HI test using antigen A/Legok/IPB-SGT/1/2004 (H5N1) and A/Ck/West Java/PWT-Wij/2006 (H5N1), seven of 163 birds (4.29%) had positive antibodies to AI. There were differences in HI titers between the two antigens. By using A/Legok/IPB-SGT/1/2004 (H5N1) antigen, two sample were strongly positive (titers ≥ 24), while the other five samples were weakly positive (titers <24). Strongly positive results were obtained from two scooty-headed bulbul (Pycnonotus aurigaster, family Pycnotidae). While the weakly positive results were obtained from a swan (Cygnus sp., family Anatidae), a duck (Anas sp., family Anatidae), a munia (Lonchura maja, family Ploceidae), a spotted-dove (Streptopelia chinensis, family Columbidae), and a black-crowned iv night heron (Nyctorax nyctorax, family Ardeidae). By using A/Ck/West Java/PWT-Wij/2006 (H5N1) antigen, the average HI titer obtained was higher. Five samples were strongly positive (Lonchura maja, Cygnus sp., Nyctorax nyctorax, and two Pycnonotus aurigaster), while samples from Anas sp. and Streptopelia chinensis were weakly positive.
Keyword: 

Judul: Metrik Einstein (Anti-) Self-Dual Berdimensi Empat dengan Metode Cartan
Abstrak: Gravitational field equation can be formulated in differential forms (differential geometry), which describes gravity as a manifestation of the curvature of space-time. Curvature of space-time is characterized by a metric that satisfies the gravitational field equation. With differential forms it can be formulated the (anti-) self-dual Einstein equation, which is a modification of the Cartan structure in (anti-) self-dual basis. Calculation of (anti-) self-dual metric with (anti-) self-dual Einstein equation is simpler than the calculation using Christoffel symbols.
Keyword: