Judul: Kajian Biofisik, Wisatawan dan Masyarakat Sekitar Lokawisata Baturaden Banyumas
Abstrak: The local government of Central Java Province sets up Banyumas Regency as tourism destination area and Lokawisata Baturaden is the most popular of all tourism object in Banyumas.
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Judul: Dampak Ekonomi dan Daya Dukung Kawasan Wisata dalam Pengembangan Lokawisata Baturraden Purwokerto, Kabupaten Banyumas
Abstrak: Lokawisata Baturraden merupakan salah satu objek wisata di Kabupaten Banyumas yang menyuguhkan keindahan alam pegunungan dari Gunung Slamet dengan beberapa pilihan atraksi wisata menarik yang bisa dinikmati, seperti pemandian air panas, sepeda air, cascade alam, kolam renang dan lainnya. Banyaknya jumlah wisatawan dari tahun ke tahun memberikan dampak ekonomi pada masyarakat sekitar namun hal tersebut juga dapat memberikan dampak negatif bagi lingkungan akibat adanya potensi berlebihnya kapasitas daya dukung kawasan. Tujuan penelitian ini yaitu: (1) Mengestimasi dampak ekonomi yang ditimbulkan dari aktivitas wisata di Lokawisata Baturraden terhadap masyarakat lokal; (2) Mengestimasi daya dukung kapasitas di Lokawisata Baturraden terhadap aktivitas wisata; (3) Mengkaji strategi pengelolaan Lokawisata Baturraden sesuai daya dukung kawasan dan memberikan manfaat ekonomi. Metode yang digunakan adalah analisis multiplier effect, analisis daya dukung kawasan, analisis SWOT dan QSPM. Hasil dari penelitian ini menunjukkan bahwa (1) Dampak ekonomi kawasan Lokawisata Baturraden berdasarkan nilai efek pengganda (multiplier effect), diperoleh nilai keynesian income multiplier yang diperoleh adalah 1.2 dan nilai ratio income multiplier tipe I dan tipe II adalah 1.7 dan 1.9; (2) Perolehan daya dukung kawasan per hari adalah sebesar 22,093 orang. Data-data yang telah didapat menunjukkan bahwa kawasan wisata Lokawisata Baturraden belum mengalami kelebihan kapasitas daya dukung kawasan pada saat low season dan sudah mengalami kelebihan kapasitas daya dukung kawasan pada saat peak season yang menunjukkan angka rasio sebesar 108.79%; (3) Alternatif kebijakan yang dihasilkan dengan analisis SWOT dan QSPM menunjukkan beberapa alternatif strategi pengelolaan adalah meningkatkan promosi wisata Lokawisata Baturraden diberbagai media cetak dan elektronik terutama pembuatan akun Baturraden di media sosial dan bekerjasama dengan travel agent untuk memperluas pangsa pasar, mempertahankan dan meningkatkan kekhasan wisata dengan potensi SDA yang dimiliki supaya tidak mengalami penurunan kualitas lingkungan, menambah produk wisata baru pada area yang sedikit aktivitas wisatawannya sehingga wisatawan tersebar di seluruh kawasan wisata.
Keyword: dampak ekonomi wisata, daya dukung kawasan, strategi pengelolaan, Lokawisata Baturraden

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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