Judul: Identification of Staphylococcus aureus from Vagina of Limousine Cattle with Repeat Breeding in BPTU-HPT Padang Mengatas
Abstrak: Repeat breeding (RB) merupakan satu diantara kasus gangguan reproduksi yang umum terjadi pada sapi yang dapat menyebabkan penurunan fertilitas induk dan angka kebuntingan. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri Staphylococcus aureus yang diduga menjadi satu diantara penyebab kasus repeat breeding pada sapi Limousin di BPTU-HPT Padang Mengatas. Sampel yang digunakan sebanyak lima ekor sapi Limousin yang mengalami RB. Spesimen berasal dari cairan vagina sapi yang mengalami RB. Metode uji yang digunakan untuk identifikasi bakteri adalah kultur, pewarnaan Gram dan uji biokimia. Hasil uji identifikasi menunjukkan bahwa pada lima sampel yang diuji tidak ditemukan bakteri Staphylococcus aureus. Namun, ditemukan bakteri lain diantaranya Micrococcus sp., Bacillus sp., Escherichia sp., Achromobacter sp., dan Alcaligenes sp. Kata Kunci: gangguan reproduksi, repeat breeding, sapi Limousin, Staphylococcus aureus, Repeat breeding (RB) is one of the common cases of reproductive disorders in cattle that could lead to decrease of calf birth and conception rate. The objectives of this study was to isolate and identify the Staphylococcus aureus which was suspected as one of the causes of repeat breeding cases on Limousine cattle in BPTU-HPT Padang Mengatas. The samples used were five Limousine cattle which suffer from RB. The specimens came from vaginal discharge of the cattle which suffer from RB. The test method used to identify bacteria are culture, Gram stain and biochemical test. The results of the identification test showed that there is no Staphylococcus aureus bacteria found over the five samples tested. However, there were other bacteria found including Micrococcus sp., Bacillus sp., Escherichia sp., Achromobacter sp., and Alcaligenes sp. Keywords : Limousine cattle, repeat breeding, reproductive disorder, Staphylococcus aureus
Keyword: repeat breeding, Staphylococcus aureus, Gangguan reproduksi, sapi Limousin

Judul: Isolasi dan Identifikasi Staphylococcus aureus pada Vagina Sapi Simmental yang Mengalami Kawin Berulang
Abstrak: Staphylococcus aureus merupakan salah satu bakteri penyebab infeksi sekunder pada sapi yang mengalami kawin berulang (repeat breeding, RB). Kawin berulang dapat menyebabkan penurunan angka kebuntingan dan kelahiran pada sapi. Tujuan dari penelitian ini adalah mengisolasi dan mengidentifikasi Staphylococcus aureus dari vagina sapi Simmental yang mengalami RB dari BPTU-HPT Padang Mengatas. Sebanyak lima spesimen bilasan vagina dikoleksi dari lima ekor sapi Simmental yang mengalami RB. Metode yang digunakan untuk identifikasi bakteri pada penelitian ini, yaitu pewarnaan Gram dan uji biokimia. Spesimen dikultur pada media blood agar (BA) dengan teknik gores T, lalu dilakukan pewarnaan Gram dan dilanjutkan dengan uji biokimia. Hasil penelitian menunjukkan bahwa tidak ditemukan bakteri Staphylococcus aureus dari kelima spesimen bilas vagina sapi yang diidentifikasi. Adapun bakteri yang teridentifikasi, yaitu bakteri genus Micrococcus dan Bacillus. Kata kunci: kawin berulang, sapi Simmental, Staphylococcus aureus, Staphylococcus aureus is a bacteria’s causes secondary infection toward the repeated breeding (RB) of cows. repeated breeding could affect the decline of pregnancy rate for the cows. This study aimed to isolate and identify the Staphylococcus aureus in Simmental’s vagina through the RB in BPTU-HPT Padang Mengatas. There were five specimens of vaginal douching collected from five Simmental cows had been through repeated breeding. There were two methods used in this research to identify the bacteria, those were Gram Staining and Biochemical test. The specimens were cultivated within the media of Blood Agar (BA) by using T-Streak technique. The next step was Gram staining, and continued by biochemical testing. The result showed that there were no Staphylococcus aureus bacteria found from those identified specimens. On the other side, the bacteria identified from those specimens were genus Micrococcus and Bacillus. Keywords: repeated breeding, Simmental cows, Staphylococcus aureus
Keyword: repeated breeding, Simmental cows, Staphylococcus aureus

Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4