Judul: Identifikasi dan Pemetaan Tingkat Kekritisan Lahan di DTA Waduk Wonorejo dengan Menggunakan Sistem Informasi Geografis.
Abstrak: Lahan kritis menurut Undang-Undang Nomor 37 Tahun 2014 tentang Konservasi Tanah dan Air adalah lahan yang mempunyai fungsi kurang baik sebagai media produksi untuk menumbuhkan tanaman yang dibudidayakan atau yang tidak dibudidayakan. Lahan kritis yang terjadi di Daearah Tangkapan Air (DTA) Waduk Wonorejo berpotensi menyebabkan penurunan produksi pertanian, erosi, sedimentasi, banjir, kekeringan, pendangkalan sungai serta berkurangnya umur waduk. Penelitian bertujuan untuk mengidentifikasi dan memetakan parameter tingkat kekritisan lahan di DTA Waduk Wonorejo. Ketersediaan data dan informasi terbaru mengenai lahan kritis dapat membantu dalam penyusunan rencana penanggulangan lahan kritis dan memberikan preferensi pengelolaan yang tepat di DTA Waduk Wonorejo sehingga kelestarian ekosistem dapat terjaga. Penentuan tingkat kekritisan lahan berdasarkan Perdirjen BPDASPS Nomor P.4/VSet/ 2013 diidentifikasi dari beberapa parameter yaitu peta penutupan lahan, kemiringan lereng, tingkat bahaya erosi, produktivitas, dan manajemen lahan. Hasil penelitian yang diperoleh menunjukkan bahwa sebaran lahan kritis dan sangat kritis di DTA Waduk Wonorejo cukup luas yaitu 429.35 ha (17.15%) dan 641.83 ha (25.63%) dari luas DTA Waduk Wonorejo tanpa memperhitungkan kelas penutupan lahan awan, bayangan awan dan badan air.
Keyword: DTA Waduk Wonorejo, lahan kritis, sistem informasi geografis

Judul: Penentuan Tingkat Kekritisan Lahan di DAS Citarum Hulu dengan Sistem Informasi Geografis
Abstrak: Daerah Aliran Sungai bagian hulu merupakan sistem penyangga yang harus dijaga kelestariannya. Informasi mengenai kekritisan lahan di suatu DAS bagian hulu sangat berguna sebagai penentu kegiatan rehabilitasi hutan dan lahan serta konservasi tanah dan air di wilayah tersebut. Tujuan penilitian adalah melakukan identifikasi dan pemetaan kekritisan lahan di DAS Citarum Hulu. Parameterparameter yang digunakan untuk menentukan kekritisan lahan yaitu penutupan lahan atau produktivitas lahan, kemiringan lereng, tingkat bahaya erosi, dan manajemen lahan. Hasil penelitian diperoleh bahwa tingkat kekritisan lahan di DAS Citarum Hulu didominasi oleh kelas kritis seluas 74 796.17 ha (32.10%). Penyebaran kekritisan lahan menyebar di tiga kawasan yaitu kawasan lindung, kawasan budidaya pertanian, dan kawasan hutan diluar kawasan lindung. Penyebaran kekritisan lahan di kawasan lindung didominasi pada kelas agak kritis seluas 13 059.35 ha (39.41%) dengan kelas sangat kritis seluas 10 689.46 ha (32.80%).
Keyword: DAS Citarum Hulu, lahan kritis, sistem informasi geografis

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
Keyword: