Judul: Karakteristik Semen Segar, Morfologi, dan Pengujian Keutuhan Membran Plasma Spermatozoa Kelinci Lop dan Rex
Abstrak: The study was conducted to investigate the quality of semen between Lop and Rex breed. Semen samples was collected by using artificial vagina and evaluated macro and microscopically. The evaluation included semen volume, pH, consistency, color, mass movement, motility, individual scores, concentration, and viability. The sperm morphology was examination using carbofuchsin eosin staining and the plasma membran integrity was determine using Hypo-Osmotic Swelling test (HOS test). Hypo-Osmotic solution was modified by mixing fructose and sodium citrate into three different osmotic pressure: 50, 100, and 150 mOsm/kg. All data were analyzed statistically using analysis of variance (ANOVA), whereas the data from HOS test were analyzed using completely randomized design (CRD) with three factor patterns. The result showed that mass movement, motility, and individual scores of Rex’s sperm was superior compare than Lop’s, but there were no difference in other parameters. The respons of plasma membrane integrity showed that Rex’s sperm was faster (15 minute) compare than Lop’s (30 minute). This fact may relate to the composition structure of the plasma membrane and seminal plasma.
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Judul: Hubungan Antara Viabilitas, Motilitas, dan Keutuhan Membran Plasma Spermatozoa Semen Beku Sapi Limousin
Abstrak: Limousine is a popular beef cattle among farmers. According to the Indonesian National Standard (SNI), the quality of frozen semen that is distributed and used to inseminate cattle must have a post-thawing motility of > 40% and an individual scoring > 2. This study aimed to test the quality of Limousine frozen semen with different parameters, namely the percentage of spermatozoa motility, live-dead spermatozoa (viability; indicator of viable sperm), the percentage of plasma membrane integrity of spermatozoa (MI) and to find the relationship between these three parameters. The motility was assessed subjectively using three evaluators, the viability was assessed with eosin nigrosin staining and membrane integrity (MI) was tested using hypo osmotic swelling (HOS) test. All was observed under the microscope and data were analyzed using the Pearson correlation SPSS 16 for windows. Data showed that the frozen semen of all four individuals were still in good condition and the post thawing motility of all bulls qualified the SNI for frozen semen of bulls. There was a positive correlation between motility and viability, motility and MI as well as viability and membrane integrity.
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Judul: Strategi Pemberdayaan Masyarakat dalam Rangka Rehabilitasi TAHURA Bukit Soeharto
Abstrak: TAHURA Bukit Soeharto merupakan salah satu bentuk kawasan konservasi alam yang terletak di wilayah Kabupaten Kutai Kartanegara dan Penajam Paser Utara dengan luasan 61.850 ha. Dalam rangka pemanfaatan kawasan TAHURA Bukit Soeharto, ditetapkan 3 (tiga) Kawasan Hutan dengan Tujuan Khusus (KHDTK) yaitu : (1) KHDTK Wanariset Samboja seluas 3.504 ha ; (2) KHDTK Pendidikan dan Latihan Kehutanan (BDK) seluas 4.310 ha dan (3) KHDTK Penelitian dan Pendidikan bagi Universitas Mulawarman Pusat Penelitian Hutan Tropis Lembab (PPHT) seluas 20.271 ha.
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