Judul: Uji Beda Ketebalan Integrasi pada Pantulan Pertama dan Kedua Hasil Deteksi Akustik
Abstrak: Bottom waters are habitat of biota and vegetation. Basic data retrieval waters using hidroakustik data retrieval in the Seribu Islands aims to analyze the differences in the thickness of the integration of the first echo (E1) and second echo (E2) based on the detection of Acoustic Backscattering Volume. The data obtained is then processed and analyzed using the program Echoview 4.0. Classification bottom waters at station 8 grabs done by Acoustic backscattering values of E1 and E2 Volumes bottom waters. Based on the data processing, in getting the results that 5 of 8 observation stations have muddy sand substrate and the other 3 in the form of sand. SV maximum value of 0,10 meters in thickness integration is -9,05 dB, the minimum value is -56,20 dB. For the integration of 0,20 meters thickness, the maximum value in the SV is equal to -8,02 dB get, with a minimum value of -54,59 dB. As for the SV maximum and minimum values on the thickness of the integration of 0,30 meters respectively are -8,02 dB and -53,32 dB.
Keyword: second echo., first echo, thickness integration, Integration, Acoustic backscattering volume

Judul: Pengukuran Hambur Balik Akustik Dasar Laut di Sekitar Kepulauan Seribu Menggunakan Split Beam Echosounder.
Abstrak: The purpose of this research is to compute the backscattering strength of the seabed by measuring volume backscattering strength (SV), bottom surface backscattering strength (SS), and the echo level (EL) from seabed using split beam echosounder. The research was conducted from 29th January to 3rd February 2011, around the Seribu Islands: Pramuka island, Panggang island, Karya island and Semak Daun island, North Jakarta. Acquisition of acoustic data was conducted using the SIMRAD EY 60 instrument. Acoustic data obtained from 9 stations simultaneously with sediment sampling. Acoustic processing data was conducted by Rick Towler program with Matlab based. The SV and SS were analyzed Manik et al, model by using. Sediment sampling station consisted of 9 stations: Pramuka island there are 1 station (Station 1), Karya island there are 2 stations (Station 2, and Station 3), Panggang island there are 3 stations (Station 4, Station 7, Station 9), and Semak Daun island there are 3 stations (Station 5, Station 6, Station 8). Sediment classified based on the sediment texture. Seabed surface sediments were separated into 3 types, they are: sand, mud, and clay. This analysis showed that the location of the 9 stations observation is dominated by sand fraction with the percentage of 80.85%. Mud and clay fractions had the average percentage value of 18.32% and 0.83%, respectively. The backscattering value (SV) of sand substrate ranged -10.62 to -18.51 dB with the average of -13.91 dB, and the muddy sand substrate ranged from -16.58 to -25.42 dB with the average -20.57 dB. The value of SS for the sand substrate ranged from -20.70 to -28.58 dB with the average value of -23.98 dB. Muddy sand substrate has a value of SS in the range of -26.64 to -35.49 dB with the average SS of -30.64 dB, from this research, the classification of seabed type using hydroacoustic technology was possible.
Keyword: 

Judul: Gene Editing  of Two Cyanide Synthesis-related Genes in Cassava using CRISPR/Cas9
Abstrak: Cassava (Manihot esculenta) contain toxic quantities of cyanogenic glucoside. Reducing the potentially toxic cyanogenic glucoside levels in cassava could be achieved by gene editing through Clustered Regularly Interspaced Short Palindromic Repeats – CRISPR associated protein 9 (CRISPR/Cas9), a high- precision genome editing technique in plants. Two cassava UDP- glucosyltransferase (UGT) paralogs, UGT85K4 and UGT85K5, are the genes catalyzing the synthesis of cyanogenic glucosides in plants. The study aimed to construct the CRISPR/Cas9 recombinant vector carrying single guide RNA (sgRNA) to edit UGT85K4 and UGT85K5 genes, and transform sgRNA CRISPR/Cas9 construct into KU50 cassava calli. Five sgRNAs were designed, considering the on/off target efficiency score and GC content. Those sgRNAs were ligated into pRGEB32 plasmid and introduced to competent Escherichia coli DH5a. The PCR colony and DNA sequencing were performed to verify the sgRNA insert. The amplicons showed the expected band size of recombinant vector (180 bp). Based on sequencing results, only one sgRNA was successfully ligated into the plasmid that will be used to edit the UGT85K5 gene. The sgRNA CRISPR/Cas9 construct in pRGEB32 plasmid was successfully transformed into Agrobacterium tumefaciens and introduced into KU50 cassava calli through co-cultivation. The overgrowth of Agrobacterium occurred after the co-cultivation phase.
Keyword: CRISPR/Cas9, Cyanogenic glucoside, Gene editing, sgRNA