Judul: Studi Kasus: Materi Fisika Untuk Smu
Abstrak: Permasalaban-permasalahan yang dibadapi dalam mempeJajari biclans Fisika. yaitu rumit dan sulit, terlalu banyak perumusan dan penghitungan secant matcmatilrn, schingga menjadi tidak menarik dan membosankan. Salah satu solusinya yaitu dengan adanya sist=m pengajaran beIbontuan komputcr UDtuk ilmu Fisika. Sistem tersebut telah diJremOOngkan oleh Yent Fitrini, 2002 denganjudul strips;. yaibl Pengemhangan SISIem Pongajaran BeIban .... KompuIer Be_is Multimedia (Sludi Kasus:MaIeri Pe~aran Fisika). Namun terdapal ia:Ierl>aIasan-etbaIasan yaitu pemberian soal-soai hanya beIbentuk piliban ganda .. ja dan kurangnya fasilitas aDimasi untuk membantu memahami Illateri dan soal yang diberikan.
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Judul: Pengembangan sistem pengajaran berbantuan komputer berbasis multimedia : Studi kasus materi pelajaran fisika
Abstrak: Metode pengajaran terus berkembang mulai dari metode sederhana sampai penggunaan teknologi, termasuk komputer. Banyak penelitian dilakukan untuk menyelidiki bagaimana teknologi komputer dapat memajukan dunia pendidikan. Komputer dalam dunia pendidikan terbagi atas bidang pengajaran dan non pengajaran. Pengajaran Berbantuan Komputer (Computer Assisted Instruction) termasuk ke dalam kategori pengajaran komputer di bidang pengajaran. Penelitian ini bertujuan untuk membangun suatu sistem pengajaran berbantuan komputer berbasis multimedia untuk mata pelajaran Fisika yang dapat digunakan oleh pelajar ataupun mahasiswa untuk membantun memahami pelajaran, memberikan alternatif cara belajar dan meningkatkan minat dan motivasi untuk mempelajari ilmu Fisika. Sistem Pengajaran Berbantuan Komputer dibangun dengan menggunakan tahapan dalam Model Perancangan Pengajaran Berbantuan Komputer. Sistem yang dibangun terdiri dari dua modul yaitu Modul Tutorial dan Modul Latihan Soal. Hasil penelitian menunjukkan bahwa penggunaan komputer sebagai metode pengajaran memungkinkan para siswa mengatur kecepatan belajar, banyaknya pelajaran, dan urutan pelajaran. Namun, penggunaan komputer sebagai alat pengajaran belum dapat menggantikan peran guru/pengajar. Hal ini disebabkan adanya faktor-faktor yang hanya dimiliki manusia seperti pemberian motivasi, bimbingan dan pendekatan. Maka komputer hendaknya dipandang sebagai pelengkap pengajaran dan bukan pengganti peran guru/pengajar.
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Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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