Judul: Kajian Histopatologi Pengaruh lmunisasi Intraperitoneal Streptococcus agalactiae Hemaglutinin Positif Pada Mencit Bunting
Abstrak: Kajian Histopatologis terhadap jaringan ambing mencit bunting yang diimunisasi S. agalactiae hemaglutinin positif dilakukan pada 6 ekor mencit balb-C yang dibagi l11enjadi 3 kelompok yaitu kelompok kontral negatif, kontral positif dan kelompok yang diimunisasi. Mencit-l11encit tersebut diimunisasi dengan suspensi bakteri utuh s.agalacliae hemaglutinin positif sebanyak 0,1 mllekor mencit melalui rute intraperitoneal. Empat hari setelah dilakukan uji tantang dengan bakteri yang sama, ambing mencit dikoleksi dan diperiksa secm'a histopatologis. Hasil pengamatan menunjukkan bahwa imunisasi tidak memberikan perlindungan terhadap uji tantang yang dilakukan. Kelenjar ambing mencit l11engalami peradangan yang ditunjukkan dengan adanya infiltrasi sel radang, epitel alveol l11engalal11i degenerasi hingga nekrosa. menyebabkan struktur alveol l11enghilang dan teljadi pembentukanjaringan lemak (jal pad) lebih dini. Uji statistik menunjukkan bahwa pembentukan jaringan ikat. jaringan lemak dan sel radang l11ennnjukkan perbedaan nyata (P<0,05) pada kontral positif, kelompok perlakuan dengan kontral negatif.
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Judul: Kajian Histopatologis Pengaruh Vaksin Streptococcus agalactiae yang Diradiasi pada Organ Hati dan Ginjal Mencit (Mus musculus)
Abstrak: This research was conducted to study the effect of irradiated vaccine Streptococcus agalactiae as attenuated vaccine (LD50) by gamma iradiation for the prevention of subclinical mastitis. The study was carried out on the liver and kidney of mice (Mus musculus). Twelve female mice were used in this study which divided into 4 groups, negative control (K); positive control (T); vaccine group (V); vaccine and challange group (VT). The treatment were done during pregnancy and challange post parturient (7 days), while vaccination gave during pregnancy with interval one week between first vaccination and boosting vaccine with 108cfu/ml/mice of S. agalactiae suspension a long four weeks. A week after parturient, mice were challange by S. agalactiae isolated from bovine subclinical mastitis case, dropped 50μl 108cfu/ml/teat on to orificium externa of mice mammary teat. One day after word followed by necropsy, samples were collected and fixed by Buffered Neutral Formalin 10% (BNF 10%) and procecced for histopathological slides. The parameters studied were counting of hepatocytes changes as degeneration, inflammation and necrosis both in central and portal vein region. These parameters also observed in kidney on glomerular and tubular part. Data were analized by ANOVA test and followed by Duncan test. The result showed that irradiated vaccine S. agalactiae did not have toxic effect on liver and kidney of mice that can be proved with have no significant different (p<0,05) compared between V group and K group include increase of degeneration, inflamatory cell, and cell death.
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Judul: Persamaan Korteweg-de Vries Orde Tinggi untuk Menggambarkan Gerak Gelombang Internal
Abstrak: The ocean can be considered of layers of fluid that each layer has a constant density. Internal waves are waves occur in the interface of every'two layers with different density. Because it occures underneath the surface of the ocean, it is invisible. One of internal waves is internal solitary waves. One of formulation that can describe intemal waves movement is KdV equation. KdV equation is derived from governing equations of ideal fluid in Lagrange formulation using asymptotic method. On this thesis, KdV equation is derived in higher order and then its solution is derived by using internal solitary waves assumption. An example of a two-layer fluid is a case will be used. By using Mathematica6 software, for this case will be obtained the coefficients in lower and higher order explicitly and the corresponding between coefficients and relative lower layer depths and fluid depths, the corresponding between coefficients and relative densities, and also observed the different between solitary waves in lower and higher order.
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