Judul: Social Support, Husband-Wife Interaction, and Marital Quality on Long-Distance Marriage
Abstrak: Pernikahan jarak jauh merupakan fenomena menarik bagi para pemerhati kehidupan keluarga, karena jarak fisik yang berjauhan diduga berpengaruh terhadap keharmonisan keluarga. Ketika salah satu pasangan tidak bisa mendampingi keluarga dalam menghadapi problematika sehari-hari, maka untuk mempertahankan kualitas perkawinan, dibutuhkan dukungan sosial dari lingkungan keluarga dan kerabat dan interaksi suami-istri yang baik antarpasangan. Penelitian ini bertujuan untuk menganalisis pengaruh dukungan sosial dan interaksi suami-istri terhadap kualitas perkawinan pada pernikahan jarak jauh. Responden dalam penelitian ini adalah 146 orang suami dan istri yang bekerja di Lembaga negara X, berasal dari keluarga utuh, sudah memiliki anak, dan sedang menjalani pernikahan jarak jauh selama minimal 6 bulan. Responden dipilih secara purposive dan pengambilan data menggunakan google form. Hasil penelitian menunjukkan dukungan sosial dan interaksi suami-istri yang dirasakan responden tergolong cukup baik. Setengah dari responden laki-laki memiliki kualitas perkawinan yang sangat baik, sementara lebih dari setengah responden perempuan memiliki kualitas perkawinan tergolong cukup baik. Hasil uji beda menunjukkan terdapat perbedaan signifikan pada interaksi suami-istri dan kualitas perkawinan dengan rataan laki-laki lebih tinggi daripada perempuan. Hasil uji korelasi menunjukkan semakin tinggi pendapatan responden, jumlah anak, dan dukungan sosial berhubungan dengan semakin tingginya kualitas perkawinan yang dirasakan. Adapun hasil uji regresi menunjukkan pada responden laki-laki, semakin kecil pendapatan, semakin banyak jumlah anak, semakin besar dukungan sosial yang dirasakan, dan semakin baik interaksi suami-istri akan semakin meningkatkan kualitas perkawinan., Long-distance marriage is an interesting phenomenon for family observers because physical distance could affect the family harmony. When one partner cannot accompany the family in dealing with daily problems, social support from the family and relatives and good husband-wife interaction between partners is needed to maintain the quality of the marriage. This study aims to analyze the effect of social support and husband-wife interaction on marital quality in long-distance marriages. Respondents in this study were 146 husbands and wives who worked at State Institution X, came from intact families, had children, and were currently in a long-distance marriage for at least 6 months. Respondents were selected purposively and data collection used the Google forms. Results showed that social support and husband-wife interaction felt by respondents were moderate. Half of male respondents had high marriage quality, while more than half of female respondents had moderate marriage quality. Comparative tests showed significant differences in interaction between husband and wife and the quality of marriage, with the average of male higher than female. Correlation test showed that higher income, number of children, and social support is associated with higher perceived marital quality. Regression test showed that for male respondents, lower income, more children, greater perceived social support, and better interaction between husband and wife will further improve marital quality.
Keyword: husband-wife interaction, long-distance marriage, marital quality, social support

Judul: Pengaruh Dukungan Sosial dan Strategi Koping terhadap Ketahanan Keluarga pada Pernikahan Jarak Jauh
Abstrak: Pengaruh Dukungan Sosial dan Strategi Koping terhadap Ketahanan Keluarga pada Pernikahan Jarak Jauh. Dibimbing oleh TIN HERAWATI dan DEFINA. Pasangan suami istri umumnya tinggal dalam satu rumah. Namun, ada kalanya keluarga tidak tinggal bersama yang disebabkan pasangan tersebut merantau untuk bekerja. Kondisi ini menyebabkan suami istri mengalami kesulitan dalam menjaga ketahanan keluarga. Tujuan penelitian adalah menganalisis pengaruh dukungan sosial dan strategi koping terhadap ketahanan keluarga pada pernikahan jarak jauh. Responden dalam penelitian ini adalah 60 istri yang menjalani pernikahan jarak jauh di eks-Karesidenan Pekalongan. Metode penelitian ini adalah non-probability sampling dengan teknik voluntary sampling. Data dikumpulkan melalui google form dengan kuesioner online. Hasil penelitian menunjukkan bahwa dukungan sosial berada pada kategori rendah, sedangkan strategi koping dan ketahanan keluarga pada kategori sedang. Strategi koping yang sering dilakukan kelurga adalah strategi koping yang berfokus pada emosi. Terdapat hubungan positif signifikan antara strategi koping dengan ketahanan keluarga. Faktor yang berpengaruh positif signifikan terhadap ketahanan keluarga yaitu lama pendidikan suami dan strategi koping., The Influence of Social Support and Coping Strategies on Family Strenght in Long Distance Marriages. Supervised by TIN HERAWATI and DEFINA. Married couples generally live in one house. However, there are times when the family does not live together because the couple migrates to work. This condition causes husband and wife to experience difficulty in maintaining family strenght. The research objective was to analyze the influence of social support and coping strategies on family strenght in long-distance marriages. Respondents in this study were 60 wives who had long-distance marriages in the ex-Residency of Pekalongan. This research method is non-probability sampling with voluntary sampling technique. Data was collected through google form with online questionnaire. The results showed that social support was in the low category, while coping strategies and family strenght were in the medium category. The coping strategy that is often used by the family is a coping strategy that focuses on emotions. There is a significant positive relationship between coping strategies and family strenght. Factors that have a significant positive effect on family strenght are the husband's length of education and coping strategies.
Keyword: dukungan sosial, ketahanan keluarga, pernikahan jarak jauh, strategi koping, coping strategies, family strenght, long distance marriage, social support

Judul: Gene Editing  of Two Cyanide Synthesis-related Genes in Cassava using CRISPR/Cas9
Abstrak: Cassava (Manihot esculenta) contain toxic quantities of cyanogenic glucoside. Reducing the potentially toxic cyanogenic glucoside levels in cassava could be achieved by gene editing through Clustered Regularly Interspaced Short Palindromic Repeats – CRISPR associated protein 9 (CRISPR/Cas9), a high- precision genome editing technique in plants. Two cassava UDP- glucosyltransferase (UGT) paralogs, UGT85K4 and UGT85K5, are the genes catalyzing the synthesis of cyanogenic glucosides in plants. The study aimed to construct the CRISPR/Cas9 recombinant vector carrying single guide RNA (sgRNA) to edit UGT85K4 and UGT85K5 genes, and transform sgRNA CRISPR/Cas9 construct into KU50 cassava calli. Five sgRNAs were designed, considering the on/off target efficiency score and GC content. Those sgRNAs were ligated into pRGEB32 plasmid and introduced to competent Escherichia coli DH5a. The PCR colony and DNA sequencing were performed to verify the sgRNA insert. The amplicons showed the expected band size of recombinant vector (180 bp). Based on sequencing results, only one sgRNA was successfully ligated into the plasmid that will be used to edit the UGT85K5 gene. The sgRNA CRISPR/Cas9 construct in pRGEB32 plasmid was successfully transformed into Agrobacterium tumefaciens and introduced into KU50 cassava calli through co-cultivation. The overgrowth of Agrobacterium occurred after the co-cultivation phase.
Keyword: CRISPR/Cas9, Cyanogenic glucoside, Gene editing, sgRNA