Judul: Species Composition and Vegetation Structure of  Lowland Forest in Pulau Rambut Wildlife Reserve, Kepulauan Seribu, DKI  Jakarta
Abstrak: Hutan dataran rendah perlu diteliti struktur dan komposisinya karena memiliki keanekaragaman hayati yang tinggi. Analisis vegetasi ditujukan untuk mengetahui komposisi jenis dan struktur vegetasi hutan dataran rendah Suaka Margasatwa Pulau Rambut. Analisis vegetasi dilakukan dengan metode jalur dan garis berpetak dengan luasan yang diamati sebesar 20.000 m2 dengan jumlah jalur pengamatan 10 jalur dengan ukuran 20 m x100 m. Data vegetasi yang diperoleh diolah untuk mendapatkan Indeks Nilai Penting (INP), Indeks Keanekaragaman Jenis Tumbuhan, Indeks Dominansi Jenis, Indeks Kekayaan Jenis dan Indeks Kemerataan Jenis. Pengambilan data abiotik seperti intensitas cahaya, ketinggian, kelerengan, suhu basah dan kering, kelembaban relatif, pH tanah, Kapasitas Tukar Kation tanah, dan tekstur tanah. Tumbuhan penyusun di lokasi penelitian terdapat 15 jenis. Kedoya mendominasi pada tingkat pohon, mengkudu mendominasi pada tingkat tiang, jeruk kingkit mendominasi pada tingkat pancang, dan kedoya medominasi pada tingkat semai. Nilai kerapatan tertinggi terdapat pada tingkat pertumbuhan semai sebesar 900500 individu ha-1 sehingga menghasilkan kurva “J” terbalik baik dalam struktur vertikal maupun horizontal.
Keyword: Faktor Abiotik, Indeks Nilai Penting, Komposisi Jenis, Struktur Vegetasi, Abiotic factor, importance value index, lowland forest, species composition, vegetation structure

Judul: Species Composition and Vegetation Structure of Mangrove Forest in Pulau Rambut Wildlife Reserve, Kepulauan Seribu, DKI Jakarta
Abstrak: Hutan mangrove di Suaka Margasatwa Pulau Rambut memiliki keanekaragaman hayati yang tinggi sehingga diperlukan penelitian mengenai komposisi jenis dan struktur vegetasi. Penelitian ini bertujuan untuk mengkaji komposisi jenis dan struktur vegetasi hutan mangrove di Suaka Margasatwa Pulau Rambut. Analisis vegetasi dilakukan dengan metode jalur dan garis berpetak dengan luasan plot contoh 8000 m2 dan berjumlah 8 jalur masing-masing dengan ukuran 10m x 100 m. Hasil penelitian menunjukkan bahwa hutan mangrove Suaka Margasatwa Pulau Rambut memiliki 9 jenis dari 5 famili dan didominasi oleh famili Rhizophoraceae. Jenis Rhizophora mucronata mendominasi pada tingkat pertumbuhan pohon dan semai. Jenis Ceriops tagal mendominasi pada tingkat pertumbuhan pancang. Nilai keanekaragaman, kekayaan, dan dominansi di hutan mangrove Suaka Margasatwa Pulau Rambut pada berbagai tingkat pertumbuhan tergolong rendah, namun nilai kemerataan jenisnya tergolong tinggi. Proporsi jumlah pohon dan permudaan membentuk kurva “J” terbalik. Stratifikasi tajuk pada hutan mangrove SM Pulau Rambut hanya mencapai stratum C (4-20 m)., Mangrove forest in Pulau Rambut Wildlife Reserve has high biodiversity, so research is needed on species composition and vegetation structure. This study aims to examine the composition of the type and structure of mangrove forest vegetation in the Pulau Rambut Wildlife Reserve. Vegetation analysis was carried out using the strip and line method with a sample plot area of 8000 m2 and a total of 8 lanes each with a size of 10 m x 100 m. The results showed that the mangrove forest of Pulau Rambut Wildlife Reserve has 9 species from 5 families and is dominated by the Rhizophoraceae family. The type of Rhizophora mucronata dominates the growth rate of trees and seedlings. The type of Ceriops tagal dominated the sapling growth rate. The value of diversity, richness, and dominance in the mangrove forest of the Pulau Rambut Wildlife Reserve at various growth rates is low, but the value of species evenness is high. The proportions of the number of trees and regeneration form an inverted “J” curve. The canopy stratification in the mangrove forest of Pulau Rambut Wildlife Reserve only reached stratum C (4-20 m).
Keyword: mangrove forest, Rambut Island, species composition, vegetation structure

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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