Judul: Pengaruh Pemberian Ekstrak Minyak Jinten Hitam (Nigella sativa) Terhadap Gambaran Mikroskopis Paruparu Mencit (Mus musculus)
Abstrak: This study was aimed to get information about the effect of blackseed (Nigella sativa) effect on the histopathology of mice lung. Twelve male mice of 4 weeks old were divided into four groups, each group consisted of three mice. Group K or negative control received aquadest, group HS 0.1 was received 0.1 ml blackseed oil, group HS 0.2 was received 0.2 blackseed oil and group HS Madu was received 0.3 ml mixed of blackseed oil and honey (1:20) for two months. At the end of the experiment, the mice were euthanized by atlanto occipital dislocation and necropsied to collect the lung sample. The lungs were processed to prepare the histopathology slides with Hematoxyllin-Eosin and Periodic Acid Schiff (PAS) stain. The parameters observed include counting percentage of bronchus and bronchial presenting exudates, counting the goblet cell between the respiratory epithelial cell, calculating the BALT area, counting the limfoid cell density of the BALT, counting the congestion, hemorrhage, inflammation and emphysema area on the certain fields of view, measuring the thickness of smooth muscle surround bronchial using Image J® for Microsoft® Windows®. Quantitative data were analyzed using SPSS® 16.0 software and ANOVA test followed Duncan test. The result showed that Nigella sativa treatment does not lead to increase exudation in the bronchus and bronchioles lumen,a decrease in the activity of goblet cells, a decrease the BALT area and an increase in the lymphoid cell density,a reduce the area of congestion and hemorrhage, inflammation and emphysema, reduction at the smooth muscle thickness. The blackseed content such thymoquinone and longifolene were thought to provide a favorable effect on lung histomorphology.
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Judul: Pengaruh Pemberian Ekstrak Minyak Jintan Hitam (Nigella sativa) terhadap Gambaran Histologi Organ Lambung dan Usus Halus Mencit (Mus musculus)
Abstrak: Habatussauda or black cumin seed (Nigella sativa) is a popular herbal medicine worldwide. The aim of this research was to observe histology changes on stomach and small intestine of mice treated with Nigella sativa oil extracts. Seventy two 4-week-old mice, consisting of thirty six male and female, were divided each into four groups. Group 1 (control) were treated with 0.1 ml of aquadest, group 2 with 0.1 ml of N. sativa oil extract, group 3 with 0.2 ml of N. sativa oil extract, and group 4 with 0.3 ml of combined N. sativa oil etxract and honey. All treatment were done orally everyday for two months period. Afterwards, mice were euthanized by atlanto-occipitale dislocation for organs sampling. Stomach and small intestine were fixed in 10% Buffered Neutral Formaline (BNF), embedded in paraffin, then stained with haematoxylin and eosin (HE) and periodic-acid schiff (PAS). The results showed that N. sativa treatment significantly increased (P<0.05) the number of parietal and chief cells, and reduced the number of mucous cells and infiltrating inflammatory cells in stomach; while, increased height of villi, increased number of crypts, reduced number of inflammatory cells, and reduced number of goblet cells were observed in small intestine, as compared with those in control. Additionally, there were no significant differences (P>0.05) in all parameters between male and female mice. These data may suggest cell protection and inflammatory cells suppresion activities of N. sativa treatment in gastrointestinal tracts.
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Judul: Cycle Hamilton pada Graf Lengkap, Graf Regular, dan Graf 2-Connected 4-Regular Berorder Kurang dari Sepuluh.
Abstrak: Suatu graf disebut graf Hamilton apabila graf tersebut memuat cycle Hamilton, yaitu cycle yang melewati setiap vertex tepat satu kali. Menentukan keberadaan cycle Hamilton pada suatu graf dapat menggunakan syarat cukup yang berupa teorema-teorema yang telah diperkenalkan sejak tahun 1950-an. Beberapa syarat cukup yang dibahas dalam karya ilmiah ini adalah syarat cukup yang berhubungan dengan graf lengkap dan graf regular. ,,,
Keyword: graf Hamilton, graf regular, graf lengkap, graf 2-connected 4-regular.