Judul: Produksi Antibodi Poliklonal Antiekskretori/Sekretori (E/S) Fasciola gigantica Isolat Asal Domba Dan Kerbau Pada Kelinci
Abstrak: The research was designed to study method of polyclonal antibody (Ig G) anti-Excretory/Secretory (E/S) Fasciola gigantica production in rabbit. Two antigens of E/S Fasciola gigantica (E/S Fasciola gigantica from buffalo and E/S Fasciola gigantica sheep) were immunized to rabbit, at dose level 150 μg/animal. Freund’s adjuvant added to antigen with the equal volume (1:1). The first immunization was done intravenous (i.v) without adjuvant. Second immunization were gave subcutaneous (s.c) with combination of antigen-Freund’s adjuvant complete. The third until sixth immunizations were done subcutaneous (s.c) with combination of antigen-Freund’s adjuvant incomplete. Blood samples were collecting every one week after the third immunization. To evaluated antibody anti Excretory/Secretory (E/S) Fasciola gigantica development, the sera tested using Agar Gel Precipitation Test (AGPT). The result showed that the rabbit immunized by E/S protein of Fasciola gigantica from sheep, able to develop antibody faster (4 weeks post infection) than the rabbit which immunized by E/S protein of Fasciola gigantica from buffalo (12 weeks post infection). In the same time, cross reactivity between antigens appear from the antibody that produced from each rabbit.
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Judul: Optimasi Penggunaan IgY Dan IgG Sebagai Antibodi Penangkap Dan Pendeteksi Antigen Ekskretori Sekretori (ES) Fasciola gigantica Dalam Uji Elisa
Abstrak: Fasciolosis is a parasitic disease of domestic ruminant that cause high economic loss. Currently diagnosis of fasciolosis relies on microscopic examination of Fasciola sp. eggs in fecal sample. Serological test such as ELISA has been developed to improve the diagnostic method of fasciolosis. This study was designed to optimize the use of polyclonal antibodies (IgY and IgG) as capture antibody and detection antibody in ELISA assay to detect excretory secretory (ES) of Fasciola gigantica. The conformation was done in two models which compared IgY (9,57 mg/ml) and IgG (3,75 mg/ml) as capture antibody. The first conformation was IgY as capture antibody in 1:1000 dilution and IgG as detection antibody in 1:100, 1:1000, and 1:10000 dilutions. The second conformation was IgG as capture antibody in 1:1000 dilution and IgY as detection antibody in 1:100, 1:1000, and 1:10000 dilutions. The results showed that IgY as capture antibody in 1:1000 dilution and IgG as detection antibody in 1:100, 1:1000, 1:10000 dilutions were able to capture ES antigen 3,87; 2,85; and 1,65 times than cut off value. The other conformation with IgG as capture antibody in 1:1000 dilution was able to capture ES antigen as much as 1,91 times than cut off value with IgY as detection antibody in 1:100 dilution. This showed that the good optimation ELISA was IgY as capture antibody in 1:1000 dilution and IgG as detection antibody in 1:100 dilution.
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Judul: Budaya Perkawinan Anak dan Kerentanan Rumah Tangga yang Ditimbulkan pada Masyarakat Madura
Abstrak: Kasus perkawinan anak masih banyak ditemukan di Indonesia, salah satunya yaitu di Madura. Perkawinan anak di Madura merupakan suatu budaya yang dianggap sah meskipun bertentangan dengan syarat Undang-Undang Perkawinan dan Undang-Undang Perlindungan Anak. Penelitian ini bertujuan untuk menganalisis budaya perkawinan anak dan kerentanan rumah tangga. Metode yang digunakan dalam penulisan adalah pendekatan kuantitatif yang didukung oleh data kualitatif dengan teknik pemilihan responden secara purposive. Unit analisis penelitian ini adalah rumah tangga. Hasil penelitian menunjukkan perkawinan anak memiliki kerentanan yang lebih besar dari pada perkawinan non anak. Di antara pasangan perkawinan anak, pada kerentanan ekonomi jenis pasangan yang paling rentan adalah anak perempuan dengan laki-laki dewasa, pada kerentanan sosial dan psikologi jenis pasangan yang paling rentan adalah anak perempuan dengan anak laki-laki.
Keyword: perkawinan anak, karakteristik, kerentanan