Judul: Nilai gizi daging, bobot potong dan bobot karkas domba jantan pada komposisi rumput gajah dan konsentrat yang berbeda
Abstrak: Sheep farmers usually set the amount of feed given to their sheep based on the amount of dry matter required by the sheep. Practically, it is assumed, on the average, about 5 % of sheep body weight. On the field, sheep feed was cornpod of grass and concentrate. Different ratio of gras-concentrate might be able to supply the same amount of dry matter, however, might supply different nutritional composition Consequently, different gmwxncentrate ratio, which has certain nutritional supply, results in different meat nutritional value.
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Judul: Hubungan Kondisi Fisiologis Domba Ekor Gemuk Jantan dan Palatabilitas Limbah Tauge sebagai Ransum Selama Penggemukan
Abstrak: Sheep farming has been increasing to meet demand of animal protein for human needs. In the livestock business, feed cost is the largest cost. In this study, bean sprouts waste which have no economic value was used as a sheep feed to increase sheep productivity. Productivity of sheep was affected by internal factors and external factors. The external factors are physiological responses and the internal factors are nutrient contents of feed. Bean sprout waste contains 13,63% of crude protein and 49,44% of crude fiber which are useful for sheep feed. The treatment level were P1 (100% concentrate), P2 (75% concentrate + 25% bean sprout waste), P3 (50% concentrate + 50% bean sprout waste), and P4 (25% concentrate + 75% bean sprout waste). The result showed that physiological response and palatability were increased along with the increasing of bean sprouts waste in the feed content. In this research, there were no significant relationship between palatability and physiological response.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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