Judul: Induksi mutasi krisan (Dendranthema grandiflora tzvelev.) melalui iradiasi planlet
Abstrak: Penelitian ini dilakukan dengan tujuan mendapatkan mutan krisan melalui perlakuan iradiasi sinar gamma dengan bahan planlet. Penelitian dilaksanakan di Instalasi Penelitian Tanaman Hias Cipanas Jawa Barat dan Pusat Penelitian dan Pengembangan Teknologi Isotop dan Radiasi Badan Tenaga Nuklir Nasional (BATAN) Pasar Jum'at Jakarta darı Bulan September 1999 sampai September 2000. Penelitian menggunakan Rancangan Acak Lengkap pola faktorial dengan dua faktor dan tiga ulangan. Faktor pertama adalah genotipe krisan yang terdiri atas tujuh varietas: Saraswati, Sekartaji, Dewi Sartika, Kartini, Chandra Kirana, Larasati dan Cut Nyak Dien. Faktor kedua adalah dosis iradiasi sinar gamma dengan delapan taraf, yaitu 0, 10, 12.5, 20, 25, 30, 35, dan 40 Gy. Iradiasi sinar gamma dilakukan dua kali dengan dosis yang berbeda. Iradiasi sinar gamma pertama diaplikasikan pada planlet krisan yang berumur 30 hari sejak subkultur terakhir dengan dosis 0, 10, 12.5, 20, 25, 30, 35 dan 40 Gy. Sehari setelah aplikasi sinar gamma, seluruh planlet disubkulturkan dalam media Murashige and Skoog yang mengandung 0,1 mg/l IAA dan disimpan di dalam ruang kultur. Setelah 8 minggu sejak subkultur, planlet yang hidup diradiasi kembali dengan dosis 15 Gy. Kemudian disubkulturkan kembali dalam media Murashige and Skoog yang mengandung 0,1 mg/L IAA dan disimpan dalam laboratorium kultur jaringan selama 8 minggu. Subkultur dilakukan dengan cara menanam tiap ruas batang yang masing-masing memiliki satu mata tunas aksilar. Selanjutnya seluruh planlet diaklimatisasikan selama 2 minggu di rumah kaca. Setelah itu planlet ditanam dalam polybag berdiameter 23 cm dan dipelihara hingga berbunga. Peubah yang diamati meliputi persentase kematian tanaman, tinggi tanaman, jumlah ruas, lebar daun, jumlah bunga, diameter bunga, diameter tangkai bunga, frekuensi kejadian mutan dan penyimpangan morfologi vegetatif…dst
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Judul: Induksi Keragaman Dua Varietas Krisan (Dendranthema grandiflora Tzvelev) Dengan Iradiasi Sinar Gamma Secara In Vitro
Abstrak: The research aims were to study the effect of gamma irradiation doses on Chrysanthemum (Dendranthema grandiflora Tzvelev) and increasing the genetic diversit. The research were conducted at IPB Tissue Culture Laboratory, BATAN Laboratory, and IPB Micro Tehnique Laboratory, during February–November 2010. This research used completely randomized design with two factors. The first factor is varieties of Chrysanthemum, including Dewi Ratih and Puspita Nusantara. The second factor is five doses of gamma irradiation (0, 20, 40, 60, 80 Gy). The result showed that the interaction between varieties with doses of gamma irradiation affected growth of plants and the number of chloroplast in guard cell. The number and size (length and width) of stomata were influenced by a varieties and and irradiation dose as a single factor. Increasing doses of gamma irradiation could increase the length and width of stomata. LD50 of krisan planlets were obtained 22.22 Gy for Dewi Ratih and 22.85 Gy for Puspita Nusantara. The gamma radiation dose 20 Gy produced 13 potential mutant on Dewi Ratih and Puspita Nusantara varieties. Putative mutants characteristic showed small leaves and no serrated, reddish stems, stunted, rosette, and variegata leaves.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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