Judul: Identifikasi Keragaman Gen DGAT1|EaeI pada Sapi Friesian Holstein dengan Metode PCR-RFLP
Abstrak: Diacylglycerol O-acyltransferase 1 (DGAT1) gene is one of major genes having an important role in milk fat synthesis. This research was aimed to identify DGAT1 gene polymorphism using EaeI enzyme by PCR-RFLP method. Identification was done in Holstein Friesian cattle from Balitnak (86 cows and 12 calves) and BIB Lembang (21 bulls). The length of PCR product was 411 bp. Genotyping resulted two types of alleles, namely K (411 bp) and A (203 and 208 bp); and three genotypes, namely KK (411 bp), KA (203, 208 and 411 bp) and AA (203 and 208 bp). For both locations, genotype frequency of KA (0.62) was higher than KK (0.35), while genotype frequency of the AA was very low (0.03). The allele frequency of K (0.66) was higher than A (0.34). Gene frequency of HF bulls at BIB Lembang was in Hardy-Weinberg equilibrium (x2 obs<x2 exp), but that of HF cows at Balitnak was disequilibrium. Heterozygosity of HF cattle at both locations was relatively high (Ho>He). DGAT1 gene of the observed HF cattle was polymorphic.
Keyword: PCR-RFLP, Holstein Friesian, gene polymorphism, DGAT1 gene

Judul: Identifikasi Keragaman Gen ATP Binding Cassette (ABCG2|PstI) pada Sapi Friesian Holstein dengan Metode PCR-RFLP
Abstrak: ATP Binding Cassette (ABCG2) has effects on milk production and composition. Reseptor which is encoded by the ABCG2 gene on alveoli epitel of mammary gland will bind its specific substrates, such as riboflavin, that are actively secreted, so milk will be rich of riboflavin. This study was aimed to identify the ABCG2 gene polymorphism in Holstein Friesian (HF) by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. This study used HF cattle by 224 heads from seven locations (BBIB Singasari, BIB Lembang, BPPTU Baturaden, BET Cipelang, BPPT-SP Cikole, KPSBU Pasir Kemis, and Cilumber). Data were analyzed for genotype frequency, allele frequency, and PIC value. Genotyping analysis resulted two alleles, namely A allele (292 bp) and C allele (268 bp, 24 bp); that resulted three genotypes, namely AA, AC, and CC. Analysis of genotype frequency resulted that AA homozygous was very highest (0.964) vs AC heterozygous (0.031) and CC homozygous (0.004); with allele frequency (A = 0.98; C = 0.02). PIC value ranged 0.000-0.189. This study concluded that ABCG2|PstI were polimorphic in 2 tested populations, the exception of monomorphic was in BBIB Singasari and BET Cipelang.
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Judul: Pengukuran Engagement pada Permainan Orbital-X, Permainan Pertahanan Menara Sirkular
Abstrak: Jenis permainan pertahanan menara (tower defense) adalah sebuah gaya permainan yang memiliki tantangan tersendiri untuk menyusun sebuah strategi melawan musuh dengan menempatkan menara pertahanan yang tepat. Permainan dalam tugas akhir ini adalah permainan pertahanan menara yang memiliki konsep berbeda, yaitu dengan menaruh menara pertahanan di posisi tengah dan menara lainnya berputar sirkula. Sebelum permainan disebarkan, dibuat 2 buah kuesioner, yaitu kuesioner pra-permainan dan pasca permainan untuk mengukur engagement pemain. Kuesioner pra-permainan diberikan kepada 5 orang. Kemudian, mereka diberi waktu untuk memainkan Orbital-X selama satu minggu. Setelah itu, kelima responden diberikan kuesioner pasca permainan untuk mengukur engagement ketika bermain. Hasil pengukuran engagement pada aspek immersion, pemain sangat setuju dengan pertanyaan “Saya dapat dengan mudah menggunakan kontrol Orbital-X dan saya dapat menguasainya dengan cepat” dan pemain sangat tidak setuju dengan pertanyaan “Interaksi yang terjadi dalam dunia game yang saya mainkan sama halnya seperti saya berinteraksi di dunia nyata”.
Keyword: Engagement, Gameplay, Immersion, Permainan, Pertahanan Menara