Judul: Kematian Satwa Liar Akibat Tertabrak (Road Mortality) di Taman Nasional Rawa Aopa Watumohai Sulawesi Tenggara
Abstrak: Jalan poros provinsi antara Kabupaten Bombana dengan Kabupaten Konawe Selatan sepanjang 22.4 km di Taman Nasional Rawa Aopa Watumohai (TNRAW) membelah kawasan menjadi dua bagian sehingga rawan menjadi tempat perlintasan satwa liar. Tujuan penelitian ini mengidentifikasi jenis satwa yang mati akibat tertabrak kendaraan di jalan poros TNRAW serta menghitung korelasi antara jumlah satwa yang tertabrak dengan jumlah kendaraan yang melintas. Pengambilan data dilakukan dengan menelusuri jalan sebagai bentuk transek garis dengan kecepatan konstan pada tanggal 15-28 Februari 2020 dan 15-28 Maret 2020. Data ini kemudian ditambah dengan data hasil pengamatan pihak TNRAW tahun 2019. Diperoleh 620 kasus satwa tertabrak kendaraan (data TNRAW 91 kasus dan data penelitian 529 kasus). Secara total, jenis satwa liar yang teridentifikasi tertabrak adalah 53 spesies yang terdiri dari 29 jenis aves, 6 jenis mamalia, 5 jenis amfibi, dan 13 jenis reptil. Jumlah kelompok satwa paling banyak tertabrak berturut-turut adalah amfibi (dominasi jenis Ingerophrynus biporcatus), aves (dominasi Lonchura malacca), mamalia (dominasi Rattus argentiventer), dan reptilia (dominasi Varanus salvator). Tabrakan umumnya berada di sekitar habitat savana. Diperoleh korelasi positif antara jumlah satwa yang tertabrak dan jumlah kendaraan yang melintas (r = 0.721, n = 14, P = 0.004). Untuk mengurangi terjadinya kematian satwa akibat tertabrak kendaraan disarankan agar membuat koridor untuk penyeberangan satwa liar, membatasi kecepatan laju kendaraan di areal “hot spot” tempat banyak satwa tertabrak, serta penambahan papan pengumuman dan rambu jalan di sepanjang jalan poros.
Keyword: Jalan poros, Mortalitas di jalan, Satwa liar, Taman Nasional Rawa Aopa Watumohai

Judul: Pemanfaatan Satwa Liar oleh Masyarakat Kawasan Taman Nasional Danau Sentarum
Abstrak: Taman Nasional Danau Sentarum memiliki keanekaragaman satwa liar yang tinggi. Penelitian ini bertujuan untuk mengidentifikasi dan mendeskripsikan satwa liar yang dimanfaatkan, bentuk dan cara penangkapan satwa liar, perburuan satwa liar, cara pengolahan satwa aliar, dan nilai manfaat ekonomi satwa liar masyarakat TNDS. Pengambilan data dilakukan di Desa Pulau Majang dan Desa Tinting Seligi, Kapuas Hulu, Kalimantan Barat. Pengambilan sampel dilakukan menggunakan metode snowball sampling. Pengumpulan data dilakukan dengan metode pengamatan lapang (observasi), wawancara dan studi pustaka serta analisis data menggunakan metode analisis deskriptif kualitatif. Hasil penelitian ini menemukan bahwa Masyarakat Pulau majang memanfaatkan 7 jenis satwa liar (1,3%) sedangkan masyarakat Tinting Seligi memanfaatkan 13 jenis satwa liar (2,41%) dari keseluruhan jenis mamalia, burung dan herpetofauna TNDS. Masyarakat desa Tinting Seligi dan Pulau Majang dalam penangkapan satwa liar dilakukan secara individu dan berkelompok 3-7 orang. Masyarakat melakukan penangkapan satwa liar pada siang dan malam hari. Babi hutan merupakan satwa yang paling banyak dimanfaatkan untuk diambil nilai ekonominya karena satwa ini sangat diminati dagingnya untuk dikonsumsi masyarakat dan digunakan untuk keperluan adat., Danau Sentarum National Park has a high diversity of wildlife. This study aims to identify and descriribe wild animals that are used, forms and methods of catching wild animals, hunting wild animals, processing of wild animals, and the economic value of wild animals for the people of DSNP. Data collection was carried out in Pulau Majang Village and Tinting Seligi Village, Kapuas Hulu, West Kalimantan. Sampling was carried out using the snowball sampling method. Data collection was carried out using field observation methods (observations), interviews and literature studies and data analysis using descriptive qualitative analysis methods. The results of this study found that the Majang Island community utilized 7 species of wild animals (1,3%) while the Tinting Seligi community utilized 13 species of wild animals (2,41%) of all mammals, birds and herpetofauna in the DSNP. The people of Tinting Seligi and Pulau Majang villages catch wild animals individually and in groups of 3-7 people. The community catches wild animals during the day and night. Wild boar is the most widely used animal for its economic value because this animal is in great demand for its meat to be consumed by the community and used for customary purposes.
Keyword: Pemanfaatan, satwa liar, Taman Nasional Danau Sentarum

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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