Judul: Cloning and Structure Prediction of C-Type Lectin 19 (CTL-19) from Silkworm (Bombyx mori L.)
Abstrak: Bombyx mori L. is a highly significant insect due to its silk production affects several industries. Unfortunately, B. mori farming has recently experienced mortality issues caused by pathogenic infectious diseases. Despite the extensive study of innate immunity of B. mori, information regarding the molecular functions and mode of actions of C-type lectin 19 (CTL-19) in the innate immunity of B. mori remains limited. Therefore, this study aimed to predict the structure of the B. mori CTL-19 also clone and produce the recombinant protein to better understand its role in the silkworm's innate immunity. The open reading frame (ORF) of the CTL-19 gene (945bp) was successfully amplified and cloned to pGEM®-T Easy Vector. The mature protein consists of 294 amino acids with 2 CRDs, 9 conserved cysteine, and two specific carbohydrate binding motifs. Protein threading demonstrated to be superior in order to predict the ligand binding site in comparison to homology modelling. The predicted molecular weight is 33.26 kDa, with pI value of 5.70. Furthermore, molecular docking analysis reveal that BmCTL-19 have ability to bind to various carbohydrate residues. The BmCTL-19 gene will be sub clone into an appropriate expression vector and produce purified recombinant protein to evaluate its properties.
Keyword: Innate immunity, Carbohydrate Binding Motif, Homology modelling, Protein Threading, Molecular docking

Judul: dentification of Non Molting Dwarf (nmd) on Mutant Silkworm (Bombyx mori) of Japan Strain a47 Through Positional Cloning Method.
Abstrak: Persilangan antar strain ulat sutera seringkali menimbulkan individu mutan gagal molting. Kejadian mutan non molting dwarf (nmd) mengakibatkan kematian pada sebagian populasi di usaha persuteraan alam. Mutan tersebut terjadi pada populasi larva instar pertama B. mori. Penelitian ini bertujuan mengidentifikasi ekspresi gen dan lokasi pasangan basanya yang bertanggung jawab terhadap pembentukan mutan nmd pada larva B. mori. Ulat sutera strain normal (p50T) disilangkan dengan ulat sutera mutan nmd (a47) Jepang hingga didapatkan generasi F2 melaui metode positional cloning. Karakterisasi basa DNA dianalisis langkah demi langkah melalui tahap purifikasi DNA hingga analisis PCR. Penerapan primer spesifik dapat mempersempit lokasi pasang basa DNA yang mengekspresikan mutasi nmd. Hasil penelitian menunjukkan bahwa lokasi ekspresi gen mutasi nmd berada pada kromosom 9 dengan kisaran pasangan basa 9330803 bp - 12075121 bp (2.7 mb) yang mencakup 89 kandidat gen di dalamnya. Satu gen kandidat diprediksi penyebab mutasi nmd, adalah KWMTBOMO05189. Gen tersebut diduga berkaitan dengan dn-Ras yang menghambat pengaturan pelepasan hormon ekdison., Crosses between silkworm strains usually emerged new mutant individuals that have non-molting conditions. The incidence of non-molting dwarf mutants (nmd) resulted in population mortality of up to half of population on natural silk factory. This nmd mutant was found in the population of first instar larvae of B. mori. This study aims to identify the gene expression and location of the base pairs that are responsible for the condition of the nmd mutant in B. mori larvae. Normal strain silkworms (p50T) were crossed with Japanese nmd (a47) mutant silkworms to obtain F2 generation through the positional cloning method. DNA base pairs characterization was analyzed step by step through the DNA purification phase to PCR analysis. The application of specific primers can narrow down the location of the DNA base pairs which express the nmd mutation. The results showed that the location of the nmd mutation gene expression was on chromosome 9 with a base pair range of 9330803 bp - 12075121 bp (2.7 Mb) which included 89 candidate genes on that range. One candidate gene predicted to express the nmd mutation is KWMTBOMO05189. This gene is thought to be related to dn-Ras which inhibits the regulation of ecdysone hormone.
Keyword: Gene Expression, non-molting, mutation, spesific primers

Judul: Perencanan Lanskap Sunda Kalapa, Jakarta Utara Sebagai Kawasan W isata Sejarah
Abstrak: Perkembangan kota Jakarta dari waktu ke waktu sangatlah cepat, sehingga menyebabkan kota-kota tua banyak yang tergusur untuk dijadikan daerah baru yang lebih modern. Apabila hal tersebut tidak ditanggulangi secara dini maka keberadaan Kota Lama Jakarta akan hilang. Hal inilah yang melatar belakang untuk dilakukan tindakan perlindungan dan revitalisasi kawasan bersejarah Sunda Kalapa.
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