Judul: Analisis Kelekatan Teman Sebaya dan Kemandirian pada Remaja Perdesaan serta Kaitannya dengan Temperamen Remaja
Abstrak: Penelitian ini bertujuan untuk menganalisis hubungan antara kelekatan teman sebaya dan kemandirian pada remaja perdesaan serta kaitannya dengan temperamen remaja. Desain penelitian menggunakan cross sectional study. Penelitian dilaksanakan di Kecamatan Ciampea, Kabupaten Bogor. Contoh dalam penelitian ini adalah remaja yang diasuh langsung oleh ibunya, dari tiga SMP terpilih di lokasi penelitian. Jumlah contoh dalam penelitian adalah 100 remaja yang terpilih melalui cluster random sampling. Hasil menunjukkan bahwa semakin dominan temperamen dalam aspek kemampuan mengontrol emosi (effortful control) dan temperamen dalam aspek keinginan akan kehangatan serta kedekatan (affiliativeness) maka semakin lekat remaja dengan teman sebayanya. Selanjutnya, semakin meningkat temperamen dalam aspek mengontrol emosi (effortful control) dan kelekatan teman sebaya maka semakin meningkat kemandirian remaja. Hasil lainnya menunjukkan semakin dominan temperamen remaja terhadap mudahnya mengalami stress (negative affect) maka semakin menurun kemandirian remaja. Hasil uji regresi menunjukkan bahwa penurunan skor temperamen negative affect dan peningkatan skor kelakatan teman sebaya secara konsisten berpengaruh terhadap peningkatan skor kemandirian remaja perdesaan.
Keyword: kelekatan teman sebaya, kemandirian, remaja, temperamen

Judul: Pengaruh Metode Sosialisasi dan Kelekatan Teman Sebaya terhadap Kekuatan Karakter Anak Usia Sekolah pada Keluarga Perdesaan
Abstrak: Penelitian ini bertujuan untuk menganalisis pengaruh metode sosialisasi dan kelekatan teman sebaya terhadap kekuatan karakter anak usia sekolah pada keluarga perdesaan. Penelitian ini menggunakan desain cross sectional study. Pengumpulan datanya dengan teknik wawancara dan menggunakan kuesioner terstruktur. Kerangka contoh penelitian adalah keluarga lengkap yang memiliki anak kelas 4 dan 5 yang bersekolah di dua SD Negeri di Kecamatan Pamijahan, Kabupaten Bogor. Sebanyak 100 keluarga dengan responden ibu dan anak dipilih secara acak proporsional. Hasil menunjukkan bahwa metode sosialisasi sosiokultur dan pendampingan mempunyai rata-rata tertinggi dibandingkan metode sosialisasi lain. Rata-rata tertinggi pada kelekatan teman sebaya ditemukan pada dimensi kepercayaan. Hasil model regresi menunjukkan bahwa hanya usia dan variabel kelekatan dengan teman sebaya yang berpengaruh nyata terhadap kekuatan karakter anak usia sekolah pada keluarga perdesaan.
Keyword: Character Strength, Peer Attachment, School-age Children, Socialization Methods, Rural Families, Human ecology, Cross sectional study, Family school partnership

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
Keyword: