Judul: Reverse Transcription Loop-Mediated Isothermal Amplification Primer Design for SARS-CoV-2 Detection Applications
Abstrak: Reverse transcription loop-mediated isothermal amplification (RT-LAMP) merupakan metode deteksi baru yang dapat menangani pelacakan kasus Covid-19 secara cepat, sederhana, murah, dan akurat. Tujuan penelitian ini adalah mendesain primer RT-LAMP menggunakan sekuen genom SARS-CoV-2 yang berasal dari Indonesia dan beberapa negara lain yang mewakili lima benua di dunia, serta mewakili lima variant of concern. Metode yang digunakan adalah preparasi sekuen, uji homologi, identifikasi daerah target, desain primer, identifikasi primer, dan uji spesifitas primer secara in silico. Hasil penelitian diperoleh set primer T1_9 dengan urutan basa F3: CACTGAGACTCATTGATGCTATG, B3: CCAACCGTCTCTAAGAAACTCT, F2: GTTCACATCTGATTTGGCTACT, F1c: GAAGTCAACTGAACAACACCACCT, B2: CCTTCCTTAAACTTCTCTTCAAGC, B1c: GTGGCTAACTAACATCTTTGGCACT, LB: TGAAAAACTCAAACCCGTCCTTG memenuhi parameter ideal dan memiliki spesifitas terbaik sehingga dapat digunakan untuk uji lanjutan secara in vitro menggunakan metode RT-LAMP.
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Judul: In Silico LAMP Primer Design for Pig Gene Detection Applications in Processed Meat Products
Abstrak: Kasus pencemaran daging babi pada produk olahan daging meningkatkan isu pentingnya identifikasi kehalalan pangan. Salah satu metode identifikasi yang dapat digunakan yaitu loop-mediated isothermal amplification (LAMP). Tujuan penelitian ini adalah mendesain primer LAMP menggunakan DNA mitokondria babi pada gen 12S rRNA, 16S rRNA, dan sitokrom b secara in silico. Metode yang digunakan adalah preparasi sekuen, penentuan dan identifikasi daerah target desain primer, desain primer menggunakan Primer Explorer V5, identifikasi primer, dan uji spesifitas primer secara in silico. Hasil penelitian diperoleh set primer gen sitokrom b dengan urutan basa F3: CCACCCCATATTAAACCAGAA, B3: GGTTGTCCTCCA-ATTCATGTT, F2: CTACGCTATCTACGTTCAATTCC, F1c: AGGATGG-AGGCTACTAGGGCTAAC, B2: AGGTCTGCTACTAGTATTCAGA, B1c: TGCCCATACTACACACATCCAAACA, LB: ATTTCGACCACTAAGTC-AATGCCT memenuhi parameter ideal dan memiliki spesifitas terbaik sehingga dapat digunakan untuk uji lanjutan secara in vitro menggunakan metode LAMP., The cases of pork contamination in processed meat products raises the issue of the importance of identifying halal food. One of the identification methods that can be used is loop-mediated isothermal amplification (LAMP). The purpose of this study was to design a LAMP primer using pig mitochondrial DNA in the 12S rRNA, 16S rRNA, and cytochrome b genes in silico. The research steps consisted of sequence preparation, determination and identification of target region primer design, primer design using Primer Explorer V5, identification of primer and in silico primer specificity test. The results of the study obtained a primary set of cytochrome b genes with the order of bases F3 CCACCATATTAAACCAGAA, B3: GGTTGTCCTCCA-ATTCATGTT, F2: CTACGCTATCTACGTTCAATTCC, F1c: AGGATG-GAGGCTACTAGGGCTAAC, B2: AGGTCTGCTACTAGTATTCAGA, B1c: TGCCCATACTACACACATCCAAACA, LB: ATTTCGACCACTAA-GTCAATGCCT meets ideal parameters and has the best specificity so it can be used for advanced testing in vitro using the LAMP method.
Keyword: 12S rRNA, 16S rRNA, cytochrome b, LAMP, processed meat  products

Judul: Manajemen Pemeliharaan Kuda (Equus caballus) Untuk Upacara Kenegaraan Dan Sarana Kesenjataan Di Detasemen Kavaleri Berkuda (DenKavkud) TNI-AD Parongpong
Abstrak: Cavalry Horse Detachement (Denkavkud) Indonesian National Army-Cavalary Parongpong is one of the Indonesian military units that utilize the horse and train it as a means of purpose of formal state ceremony and armed forces. Skills possessed by the horse cavalry was the result of care management and specialized training provided by the trainer. Aspects of care management are breeding, feeding, and management. Horses that are kept in the Denkavkud, Parongpong are stallions, mares, foals, and military horses. Total populations of horses at Denkavkud are 183 horses. Besides commited horse training (remonte), Denkavkud also did breeding program to produce horses that can be trained and used as a military horse. There are some issues contained in Denkavkud, such as some of the nutrients needed by the horses not being met from feed given. One of these is crude protein and lysine. Lactation mares was crude protein and lysine deficiency. The number of death in 2010 is higher than previous years. Based on the number of horses that died from January until June 2010 is 11 horses. The average mortality was caused by colic. Colic is caused by excessive eating, excessive drinking during hot, moldy food, and even by investment roundworms. Animal welfare in Denkavkud can be enhanced by improvement of feed given and improving the health of livestock through improved sanitation.
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