Judul: Pengaruh lamanya tahun pembukaan hutan terhadap beberapa sifat tanah podzolik merah kuning Sumatera Selatan dan kaitannya dengan proses degradasi tanah
Abstrak: Berdasarkan hal-hal tersebut di atas telah dilakukan penelitian lapang, laboratorium dan rumah kaca yang dimulai sejak tahun 1983 sampai dengan tahun 1986. Penelitian lapang dilakukan di daerah transmigrasi Batumarta, Baturaja, Sumatera Selatan yang tanahnya didominasi oleh jenis tanah Podzolik Merah Kuning. Tanah Podzolik Merah Kuning diteliti oleh karena jumlahnya yang luas tersebar di empat pulaupulau besar kepulauan Indonesia dan mempunyai potensi untuk dikembangkan menjadi lahan pertanian dalam rangka pembangunan daerah.
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Judul: Nilai permeabilitas tanah pada berbagai umur pembukaan dan sistim pengelolaan lahan podsolik merah kuning daerah transmigrasi Baturaja - Martapura Sumatera Selatan
Abstrak: Produksi optimum suatu tanaman dapat tercapai apa- bila sifat-sifat tanahnya baik. Untuk pertumbuhan ta- naman dibutuhkan air yang cukup, sehingga kehilangan air di lahan pertanian perlu dipertimbangkan. Penelitian ini dilakukan pada lahan alang-alang dari Podsolik Merah Kuning di daerah transmigrasi Baturaja-Martapura Sumatera Selatan, untuk mengetahui pengaruh umur pembukaan dan pengelolaan lahan secara te- rus menerus terhadap permeabilitas tanah. Dengan demi- kian dapat diambil langkah selanjutnya dalam sistim pembukaan dan pengelolaan lahan agar tidak terjadi penu- runan permeabilitas tanah. Pengukuran permeabilitas dilakukan pada enam ting- kat umur pembukaan lahan dengan jenis tanah, kemiringan lereng, dan sistim pertanaman yang relatif sama.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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