Judul: Case Studies on Ayahbunda, Femina and Kartini Magazine, Nova and Nakita In The Period January – December 2009
Abstrak: One important aspect of food control is food labeling and advertising control. In Indonesia, control for the food advertising is done by National Agency of Drug and Food Control (NADFC). Based on our data, many adverstisement of food products do not comply with the formal regulation on labelling and advertising. . Analysis on 925 advertisements collected, 507 of advertisements (55%) were comply with the requirement and 418 of advertisements were not comply with the requirement. Among 425 of advertisements which were not comply with requirement complementary breast feeding (50,59%) were the most dominant, followed by soft drink (13,41%), and milk and its processed product (11,7%). The most dominant category of violations is an advertisement that containing incorrect and misleading information related to nutrition, health benefits and food safety (72,86%).
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Judul: Materi konsultasi gizi dan klaim iklan makanan di majalah dan tabloid Nasional
Abstrak: Iklan produk makanan terdiri dari 27,6% makanan, 47,7% minuman, 13,8% suplemen dan 10,9% bahan makanan. Umumnya sasaran iklan adalah masyarakat umum dan wanita. Jenis klaim yang paling sering dijumpai adalah 'lezat atau enak' dan 'gizi tepat'. 'Lezat atau enak' dan 'gizi tepat' diklaim oleh 40,8% produk, 'zat dengan fungsi khusus' diklaim oleh 28,7% produk, 'praktis' diklaim oleh 6,9% pro­ duk dan 'aman' diklaim oleh 6,3% produk. Terdapat dua iklan yang melanggar pasal 35 Peraturan Menteri Kesehatan No.79/MENKES/PER/ Ill/1978 tentang Label dan Periklanan serta Keputusan Men­ teri Kesehatan Nomor 386/ MENKES/SK/IV/1994 tentang Pedoman Peiklanan Obat Bebas, Obat Tradisional, Alat Kesehatan, Kosmetika, Perbekalan Rumah Tangga dan Makanan-Minuman pada Lampiran 4 yang menyebutkan bahwa periklanan makanan tidak boleh menjurus ke pendapat, bahwa makanan yang bersangkutan berkhasiat sebagai obat. Produk di atas berarti melanggar Undang-undang Pangan pasal 33 ayat J karena telah memberikan keterangan yang tidak benar dan menyesatkan yaitu mengklaim dapat mengatasi penyakit.
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Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.