Judul: Perencanaan Lanskap Kawasan Permukiman Berbasis Mitigasi Penurunan Muka Tanah Studi Kasus Kecamatan Cengkareng Jakarta Barat
Abstrak: Kecamatan Cengkareng merupakan salah satu kecamatan yang terdapat di Kota Jakarta Barat yang kerap mengalami fenomena penurunan muka tanah. Penurunan muka tanah (land subsidence) merupakan suatu proses gerakan penurunan muka tanah yang didasarkan atas suatu datum tertentu (kerangka referensi geodesi) dimana terdapat berbagai macam variabel penyebabnya (Marfai 2006). Penurunan muka tanah dapat disebabkan oleh beberapa faktor, yaitu faktor alami, faktor pengambilan air tanah dan faktor massa bangunan. Penurunan muka tanah dapat berdampak, baik secara material maupun non-material. Untuk mengatasi dampak yang akan muncul dibutuhkan perencanaan tata ruang yang dapat meminimalisir dampak yang terjadi akibat penurunan muka tanah pada kawasan Kecamatan Cengkareng. Tujuan penelitian ini adalah menyusun rencana lanskap kawasan permukiman berbasis mitigasi bencana penurunan muka tanah. Penelitian dilakukan dengan pendekatan lanskap dalam proses perencanaan lanskap sebagaimana dikemukakan oleh Fabos (1979) yang terdiri tiga tahap, yaitu: penentuan sumber daya kritis, penentuan sumber daya bahaya dan penentuan kawasan permukiman yang dapat dikembangkan. Penelitian dilakukan untuk mengetahui karakteristik kawasan rawan bencana penurunan muka tanah, keterkaitan antara penurunan muka tanah dan bencana banjir, serta menghasilkan perencanaan tata ruang yang dapat menjadi solusi dari permasalahan penurunan muka tanah. Berdasarkan hasil analisis, rencana lanskap dituangkan kedalam empat zona yakni zona konservasi, zona rawan bencana, zona penyangga dan zona pengembangan. Setiap zona dikembangkan secara fungsional dan terintegrasi dengan kebutuhan ruang, fasilitas serta aktivitas masyarakat Kecamatan Cengkareng., Cengkareng District, located in West Jakarta City, experiences frequent land subsidence phenomenon. Land subsidence is a process of land subsidence movement based on a certain datum (geodesy reference framework) where there are various variables (Marfai 2006). This process is caused by natural, groundwater extraction, and building mass factors. This phenomenon could lead to material and non-material impacts. In order to minimize the negative effects of land subsidence in the area, there is a need for spatial planning. The aim of this research is to develop a landscape plan for residential areas based on land subsidence disaster mitigation. The process involved three stages of landscape planning as proposed by Fabos (1979) determining critical resources, determining hazardous resources and determining residential areas that can be developed. The research was conducted to identify areas prone to land subsidence disasters, the relationship between land subsidence and flood disasters, and to produce spatial planning that can mitigate the problem of land subsidence. Based on the analysis, the landscape plan is divided into four zones: conservation, disaster-prone, buffer and development zones. Each zone is developed with the needs of the community in mind, integrating space, facilities, and activities.
Keyword: Sumber Daya Bahaya, Sumber Daya Kritis, Sumber Daya Pengembangan, Penurunan Muka Tanah, Critical Resources, Development Resources, Hazardous Resources, Land Subsidence

Judul: Perencanaan Lanskap Berbasis Mitigasi Banjir di Kecamatan Jatiasih Kota Bekasi
Abstrak: Banjir merupakan suatu fenomena alam yang sering terjadi di Indonesia. Bencana ini terjadi akibat kelebihan air yang tidak tertampung lagi oleh jaringan drainase sehingga membuat genangan yang merugikan. Salah satu daerah yang sering terkena banjir adalah Kecamatan Jatiasih. Oleh karena itu diperlukan perencanaan lanskap berbasis mitigasi banjir di Kecamatan Jatiasih, Kota Bekasi. Tujuan dari penelitian ini adalah meminimalisir dampak yang disebabkan oleh banjir. Metode yang dilakukan pada penelitian ini ada 5 tahap, yaitu tahap persiapan, inventarisasi, analisis, sintesis, dan perencanaan. Analisis yang dilakukan adalah analisis legalitas, kerawanan bencana banjir, kerentanan terhadap bencana banjir, dan risiko bencana banjir. Hasil analisis menyatakan bahwa Kecamatan Jatiasih didominasi kerawanan sedang sebesar 38,10%, kerentanan sangat tinggi sebesar 59,44%, dan berisiko tinggi terhadap bencana banjir sebesar 40,48%. Berdasarkan hasil analisis tersebut selanjutnya dikembangkan konsep perencanaan dengan konsep dasar mitigasi bencana banjir secara alami. Konsep ini kemudian dikembangkan menjadi rencana lanskap. Rencana lanskap disusun berdasarkan rencana tata ruang, aktivitas, evakuasi, fasilitas, sirkulasi, dan vegetasi., Flood is a natural phenomenon that occurs frequently in Indonesia. This disaster occurred as a result of excess water that was no longer accommodated by the drainage network, thus creating an adverse puddle. One area that is often affected by annual floods is in Jatiasih District. Therefore, a landscape planning based on flood mitigation is needed in Jatiasih District, Bekasi City. The purpose of this research is to minimize the impact caused by flooding. The method used in this study consists of 5 phases starting from preparation, inventory, analysis, synthesis, and landscape planning. The analysis carried out is sensitivity analysis, analysis of vulnerability, and flood risk analysis. The results of the analysis stated that Jatiasih District is dominated by medium sensitivity of 38.10%, very high vulnerability of 59.44%, and high risk of flood disaster of 40.48%. Based on the results of the analysis then developed a planning concept with the basic concept of natural flood disaster mitigation. This concept was later developed into a landscape plan. Landscape plans are prepared based on spatial plans, activities, evacuation, facilities, circulation, and vegetation.
Keyword: flood, mitigation, landscape planning

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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