Judul: Optimasi Produksi Bibit Tanaman Kentang (Solanum tuberosum) ‘Granola’ dengan Teknik Fotoautotrofik
Abstrak: Potato is the fourth food in the world after rice, corn, and wheat. Indonesia still imports potatoes for seedlings, seeds and foods, especially for the industry. Indonesian production was law caused farmers have not been producing good quality of potatoes seed, so the demand for seed of potatoes can not filled. The efforts should be made to overcome these obstacles is use biotechnology by micropropagation for potatoes seedling. In vitro plants rarely photosynthesise; their cuticle, vascular tissue between roots and shoots and stomata do not grow and functioning so that the in vitro derived plantlets had low survival in ex vitro conditions. Photoautotrofik system have been developed to overcome the low adaptability of plantlets when acclimatization. This method uses a lower concentration of sugar as an energy source for explants and explants induced to be able to photosynthesise in the semi-open conditions. This condition is more similar to conditions on the ground, so that the explants will have a higher adaptability when the acclimatization. This research aims to study the growth of potato ‘Granola’ cultured in vitro with photoautotrophic system to provide good quality potato explants. This research was conducted at the Laboratory of Tissue Culture 2, morphological analysis of stomata was conducted at the Laboratory of Micro Technique, Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University. The research was conducted from November 2014 until April 2015. This research is consisted of two separate experiments. The first experiment used a single node explant, a second trial using shoot explants. The experiment was arranged in a Randomized Blok Design with two factors concentration of sugar and ventilation. The concentration of sugar are 5, 10, 15, 20 and 25 gL-1, the ventilaion are one and two ventilation. Increases in sucrose concentration correlated positively to the growth of Solanum tuberosum plantlets. Interaction of low sugar and additional ventilation increased the number of stomata and chloroplasts as well as narrowing the diameter of stomata. 45% plantlets grown on media with the treatment of 25 gL-1 sugar with 1 ventilation and 67% on 25 gL-1 sugar with 2 ventilation survived and could be used for seedling production. Interaction of low concentration of sugar with the addition of ventilation in second trial on all treatments significantly increased the density of stomata, number of chloroplast and causes smaller diameter of stomata. In this experiment there is no plantlets were able to survive in the acclimatization stage.
Keyword: potato, in vitro, photoautotrophic, photosynthesis

Judul: Performance and Productivity of Potato (Solanum tuberosum L.) Granola Variety from Five Generations of Tuber Seed.
Abstrak: Produktivitas kentang di Indonesia masih dapat ditingkatkan melalui penggunaan benih bermutu. Kelas benih merupakan hal yang penting, mengingat kentang diperbanyak secara vegetatif. Penelitian ini bertujuan mengetahui pengaruh penggunaan generasi benih yang berbeda terhadap keragaan dan produktivitas kentang. Penelitian dilaksanakan di Desa Aek Natolu Jaya, Kecamatan Lumban Julu, Kabupaten Toba, Provinsi Sumatera Utara pada bulan November hingga bulan Maret 2020. Rancangan yang digunakan dalam penelitian ini adalah Rancangan Kelompok Lengkap Teracak (RKLT) faktor tunggal yaitu perlakuan dari lima generasi kentang varietas Granola yaitu G0, G1, G2, G3, dan G4 dengan tiga ulangan. Hasil penelitian menunjukkan perlakuan G0, G1, dan G2 memiliki keragaan yang sama dan merupakan keragaan yang terbaik berdasarkan keragaan tinggi dan diameter batang serta memiliki produktivitas terbaik. Produktivitas mulai menurun pada perlakuan G3 dan G4. Jumlah umbi per tanaman yang paling tinggi adalah perlakuan G0 dan G1. Grade umbi A hanya terdapat pada perlakuan G2, hal ini menunjukkan bahwa G2 lebih cocok dijadikan sebagai benih sebar. Perlakuan G0, G1, dan G2 juga menghasilkan grade umbi yang lebih baik dibandingkan G3 dan G4., The Potato productivity in Indonesia can still be increased through the use of quality seeds. Seed class is important in potato production as it is propagated vegetatively. This study aimed to determine the effect of different seed generations on potato performance and productivity. The study was carried out in Aek Natolu Jaya village, Lumban Julu sub-district, Toba Regency, North Sumatra Province from November to March 2020. The design used in this study was a single factor Randomized Complete Block Design (RCBD). The treatments were five generations of Granola potato, G0, G1, G2, G3, and G4 with three replicates. The results showed that treatment G0, G1, and G2 had the same performance and achieved the best performance based on the height and diameter of the stem and had the best productivity. The productivity started to decrease in the G3 and G4 treatments. The highest number of tubers planted was achieved by G0 and G1 treatments. Grade A tubers were only found in the G2 treatment, this indicated that G2 was more suitable to be used as extension seed propagule. G0, G1, and G2 treatments also produced better tuber grades than G3 and G4.
Keyword: decrease, extension seed, grade, quality, vegetative

Judul: Infeksi Cacing Jantung Dirofilaria Immitis, Railliet & Henry, 1911 Pada Anjing
Abstrak: Cacing Dirofilaria immitis dewasa yang tinggal dalm ventrikel kanan jantung anjing, sering kali menimbulkan gejala klinis yang cukup berat. Cacing Tersebut menghasilkan mikrofilaria yang selanjutnya berkembang menjadi larva infektif dalam saluran malphigi induk semang entara, yaitu nyamuk dari genus Culex, Aedes dan Anopheles.
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