Judul: Development of Portable Spectrophotometers and Performance Evaluation of Ponceau 4R Food Colorants Substances
Abstrak: Spektrofotometer dapat dikembangkan dari komponen elektronik yang tersedia secara umum menjadi spektrofotometer portabel. Penelitian ini bertujuan mengembangkan spektrofotometer jinjing dan menentukan uji kinerja analitiknya. Spetrofotometer jinjing yang disusun oleh komponen utama yang terdiri dari lampu light emitting diode sebagai sumber cahaya, lapisan optik digital versatile disks sebagai kisi, web camera Logitech c920 sebagai detektor dan akuisisi data melalui perangkat lunak Thereminospectro. Uji kinerja spektro menggunakan larutan standar Ponceau 4R dan dibandingkan dengan spektrofotometer komersial Hitachi Spectro U-2800. Spektrofotometer jinjing dengan ukuran optimum menghasilkan spektrum larutan standar yang sebanding dari hitachi spectro u-2800. Keduanya menunjukkan linearitas yang sangat baik karena memenuhi syarat keberterimaan linearitas pada metode spektrofotometri. Dengan demikian, spektrofotometer jinjing yang dikembangkan dapat menjadi alternatif untuk spektrofotometer komersial. Kata kunci: digital versatile disks, light emitting diode, ponceau 4R, spektrofotometer, web camera, Spectrophotometers can be expanded from commonly available electronic components to portable spectrophotometers. This study aims to develop a portable spectrophotometer and determine its analytical performance test. A portable spectrophotometer composed of main components consisting of a light-emitting diode lamp as a light source, an optical layer of digital versatile disks as a grid, a Logitech c920 web camera as a detector, and data acquisition via the Thereminospectro software. Spectro performance test using standard solution Ponceau 4R and compared with a commercial spectrophotometer Hitachi Spectro U-2800. The portable spectrophotometer with the optimum size produces a standard solution spectrum comparable to that of the Hitachi Spectro u-2800. Both of them show very good linearity because they meet the requirements for linearity acceptance in the spectrophotometric method. Thus, the developed portable spectrophotometer can be an alternative to commercial spectrophotometers. Keywords: digital versatile disks, light emitting diode, ponceau 4R, spectrophotometer, web camera
Keyword: Spektrofotometer UV-VIS, Spektrofotometer jinjing, LED, DVD, Webcamera, Ponceau 4R

Judul: Pengembangan Spektrofotometer Jinjing dan Evaluasi Kinerjanya pada Zat Pewarna Tartrazina
Abstrak: Perkembangan teknologi yang pesat pada era digital seperti saat ini memungkinkan terjadinya pengembangan spektrofotometer jinjing menggunakan berbagai komponen yang lebih sederhana, seperti lampu LED (Light Emitting Diode) sebagai sumber sinar, kamera web sebagai detektor, DVD (Digital Versatile Disc) sebagai kisi difraksi, serta perangkat lunak Thereminospectrometer sebagai sistem akuisisi data. Kinerja spektrofotometer jinjing diuji dengan parameter linearitas, menggunakan larutan zat pewarna makanan tartrazina. Hasil yang diperoleh kemudian dibandingkan dengan spektrofotometer komersial Hitachi U-2800. Kinerja alat menunjukkan linearitas dengan nilai koefisien determinasi (R^2) 0,9963. Hasil yang diperoleh menunjukkan kemiripan jika dibandingkan dengan spektrofotometer pembanding tersebut. Telaah pustaka menunjukkan bahwa spektrofotometer jinjing yang telah teruji kinerjanya, bukan hanya dapat diaplikasikan dalam bidang analisis tetapi juga dalam bidang pendidikan., A rapid technology development on this digital era, provide ways to develop a portable spectrophotometer using common electric components that are simpler, cheaper, and easy to construct. The aim of this study is to develop a portable spectrophotometer using white LED (Light Emitting Diode) lamp as light source, web camera as a detector, DVD (Digital Versatile Disc) as diffraction grating, and offline software Thereminospectrometer as data acquisition system. The analytical performance is measured by linearity parameter using tartrazine dye solution. The data obtained from our portable spectrophotometer were compared to a commercial spectrophotometer Hitachi U-2800. Linearity test of portable spectrophotometer shows linearity with coefficient of determination value (R^2) equal to 0,9963. The result shows a similar value compared to commercial spectrophotometer. Literature review confirm that a portable spectrophotometer is not only applicable for analysis, but also for educational purposes.
Keyword: kamera web, spektrofotometer jinjing, spektrofotometer sinar tampak, tartrazina

Judul: Posisi Filogenetik Kura-Kura Carettochelys Insculpta  (Reptillia: Testudinata)
Abstrak: Carettochelys insculpta is an endemic freshwater turtle of southern Papua and northern Australia. Phylogenetic position of this turtle is remain unresolved until now. Most of the research conducted show close relationships of C. insculpta and members of Trionychidae family. However, some of the research show distance between C. insculpta and members of Trionychidae family. This research is conducted to analyze phylogenetic relationship between C. insculpta and other members of Testudinata using 12S rRNA, 16S rRNA, and both gene as markers. DNA samples were obtained from three different organisms of C. insculpta. Two species used as outgroups were Alligator mississippiensis and Iguana iguana. Phylogenetic analysis was carried out using Neighbor-Joining method with Felsenstein 1981 (F81) modeltest. A polytomy tree of Testudinata was produced by using 12S rRNA gene. However, analysis using 16S rRNA and both genes as markers confirmed that C. insculpta was located between the Chelonidae and the Trionychidae family.
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