Judul: Analisis Keragaman Genetik Ikan Baung (Hemibagrus nemurus) dengan Menggunakan Teknik PCR-RFLP pada DNA Mitokondria
Abstrak: This research has an objective to analyze the genetic variation among four Hemibagrus nemurus population by using PCR-RFLP of D-loop region of mitochondrial DNA. H. nemurus were collected from Cirata, Serayu, Jambi and Bogor. Four restriction enzymes were used to digest D-loop region mt-DNA, i.e. MboI, HaeIII, AluI and RsaI. Based on this analysis, these four population have seven composite haplotypes. The highest haplotype diversity was found at the population from Jambi. Population from Serayu has just one composite haplotype indicating that this population has the same ancestor. Population from Cirata and from Bogor has the same composite haplotype indicating that there was probably migration between these two populations.
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Judul: Studi keragaman genetika populasi udang galah, Macrobrachiumm rosenbergii berdasarkan Polimorfisme Mitokondria DNA
Abstrak: Indonesia dikenal sebagai pusat keberadaan spesies asli udang galah karena masih terdapat 19 spesies asli Indonesia. Udang galah Macrobrachium rosenbergii merupakan salah satu jenis udang Palaemonid air tawar yang umum dibudidayakan di Indonesia. Di alam bebas udang galah sering ditangkap di sekitar sungai, danau, dan rawa - rawa. Di Indonesia udang galah banyak terdapat di wilayah Pulau Sumatra, Pulau Kalimantan, sedangkan di Pulau Jawa, Sulawesi, dan Papua hanya sedikit ditemukan. Keragaman populasi genetik sangat penting untuk menjaga populasi, yang akan memberikan respon paralel terhadap perubahan parameter-parameter lingkungan. Pengamatan variasi atau keragaman genetik dapat dilakukan dengan dua cara yaitu melalui keragaman ale! (allelic diversity) dan heterozigositas. Estimasi keragaman genetik dapat dilakukan dengan menggunakan penanda­penanda molekuler. Salah satu penanda molekuler yang umum digunakan dalam bidang manajemen dan budidaya perikanan adalah penanda DNA mitokondria (mtDNA). Sekuens mtDNA CO-I udang galah yang diperoleh dari hasil amplifikasi memiliki panjang susunan basa antara 700-1500 bp. Dari tujuh enzim restriksi yang digunakan untuk memotong sekuens tersebut (Hae III, Rsa I, Mbo I, Taq I, Alu I, Sac I, dan Hin6 I), hanya terdapat empat enzim yang memberikan situs pemotongan pada sekuens mtDNA CO-I. Lokasi pengambilan koleksi udang galah akan mempengaruhi tingkat variasi haplotype dari tipe restriksi sekuens mtDNA CO-I udang galah.
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Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4