Judul: Teknik Pemeliharaan dan Tingkat Kesejahteraan Macan Tutul Jawa (Panthera pardus melas Cuvier, 1809) di Taman Satwa Cikembulan Garut.
Abstrak: Macan tutul jawa merupakan salah satu satwa endemik Pulau Jawa dan terancam punah. Salah satu upaya untuk mempertahankannya yaitu melalui konservasi eksitu, salah satunya di Taman Satwa Cikembulan. Penelitian ini bertujuan mengkaji teknik pemeliharaan dan tingkat kesejahteraan macan tutul jawa di Taman Satwa Cikembulan. Capaian implementasi kesejahteraan satwa diperoleh dengan menggunakan metode PKBSI (Persatuan Kebun Binatang Seluruh Indonesia). Teknik pemeliharaan termasuk aspek perkandangan belum sesuai walaupun ukuran kandang telah memenuhi ukuran minimum, pakan sudah mencukupi, kesehatan baik walaupun ketersediaan fasilitas medis masih kurang, reproduksi sudah baik dengan lahirnya anakan, dan adaptasi sudah baik. Tingkat kesejahteraan macan tutul jawa menurut pengelola 80.60% dengan kategori sangat baik sedangkan menurut pengamat yaitu 65.11% dengan kategori cukup.
Keyword: kesejahteraan satwa, macan tutul jawa, pemeliharaan, Taman Satwa Cikembulan

Judul: Wilayah Jelajah Macan Tutul Jawa dan Sebaran Satwa Mangsa Potensial di Kawasan Hutan Gunung Sawal
Abstrak: Kawasan Hutan Gunung Sawal adalah salah satu habitat macan tutul jawa. Spesies ini merupakan endemik Pulau Jawa yang terancam punah (critically endangered) berdasarkan IUCN. Penelitian ini bertujuan untuk menghitung luasan wilayah jelajah macan tutul jawa, mengidentifikasi dan memetakan sebaran satwa mangsa potensial macan tutul jawa. Penelitian dilakukan dari bulan Desember 2019 sampai Februari 2020 dengan menggunakan kamera penjebak. Hasil penelitian menunjukkan terdapat enam individu macan tutul jawa, dengan tingkat perjumpaan 2.95 foto/100 hari rekam. Berdasarkan hasil perhitungan menggunakan metode Minimum Convex Polygon (MCP) diperoleh luas wilayah jelajah individu Jantan Dewasa 1 (JD 1) sebesar 15.19 km2, Jantan Dewasa 2 (JD 2) sebesar 3.84 km2, dan Betina Dewasa 2 (BD 2) sebesar 3.82 km2. Sebaran satwa potensial mangsa macan tutul jawa cukup merata di seluruh wilayah kawasan hutan Gunung Sawal. Keragaman tertinggi satwa potensial mangsa berada di wilayah Cibuyut dengan jumlah jenis satwa mangsa sebanyak tujuh jenis. Total ditemukan 13 satwa mangsa potensial di wilayah studi yakni musang luwak (Paradoxurus hermaproditus), babi hutan (Sus scrofa), sigung (Mydaus javanensis), kijang (Muntiacus muntjak), anjing kampung (Canis familiaris), kucing hutan (Prionailurus bengalensis), tikus belukar (Ratus tiomanicus), biul slentek (Melogale orientalis), trenggiling (Manis javanica), Surili (Presbytis comata), monyet ekor panjang (Macaca fascicularis), puyuh gonggong jawa (Arborophila javanica), dan paok pancawarna (Pitta guajana). Berdasarkan indeks kelimpahan relatif, musang luwak, sigung, babi hutan, kijang, puyuh gonggong, dan anjing kampung memiliki nilai foto per 100 hari rekam tertinggi berturut-turut yakni 5.8, 1.40, 0.62, 0.62, 0.62, 0.62.
Keyword: kamera penjebak, macan tutul jawa, MCP, satwa mangsa potensial, sebaran, wilayah jelajah

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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