Judul: Effect of Multistage Extraction on the Gelatin Properties of Pangasius Fish Skin (Pangasius hypopthalmus)
Abstrak: Gelatin merupakan produk hidrolisis kolagen yang dapat membentuk gel reversibel. Salah satu bahan baku gelatin adalah kulit ikan patin. Ekstraksi tunggal gelatin menyisakan kolagen di dalam struktur kulit ikan patin sehingga perlu dilakukan ekstraksi bertingkat. Ekstraksi bertingkat bertujuan meningkatkan rendemen sekaligus mempertahankan kualitas gelatin. Produksi gelatin diawali dengan pretreatment di dalam NaOH 0,2 M dan asam asetat 0,05 M. Ekstraksi bertingkat menggunakan kombinasi suhu 55-75, 55-80, dan 65-75 oC selama 10 jam pada setiap tahap dengan pelarut akuades. Rancangan percobaan yang digunakan yaitu Rancangan Acak Lengkap (RAL) dan uji lanjut Duncan. Penggunaan metode ekstraksi bertingkat meningkatkan rendemen dibanding ekstraksi tunggal. Perbedaan perlakuan berpengaruh terhadap viskositas, kekuatan gel, dan distribusi bobot molekul gelatin. Perlakuan tidak berpengaruh terhadap pH, kadar air, kadar abu, serta setting point. Hasil terbaik diperoleh pada perlakuan ekstraksi 65-75oC yang menghasilkan rendemen (25.05±0.35%), viskositas (63.67±2.08 mps), serta kekuatan gel (255.69±2.94 bloom). Perbedaan perlakuan menyebabkan perbedaan tingkat kerusakan pada struktur stratum compactum kulit ikan patin., Gelatin is hydrolyzed collagen that can form a reversible gel. One source of gelatin is Pangasius skin. Gelatin single extraction left some collagen in Pangasius skin so that a multistage extraction is needed. Multistage extraction aims to increase yield and maintain gelatin quality. Gelatin production began with pretreatment in 0,2 M NaOH and 0,05 M acetic acid. This extraction used some combination of 55-75, 55-80, and 65-75 oC for 10 hours at each stage in distilled water. The experimental designs in this study were completely randomized design (CRD) and Duncan's advanced test. Multistage extraction improved gelatin yield from a single extraction. Different treatments affected viscosity, gel strength, and molecular weight distribution. Treatments did not affect pH values, water contents, ash levels, and setting points. The best results were obtained at 65-75 oC treatment which resulted in a yield (25.05±0.35%), viscosity (63.67±2.08 mps), and gel strength (255.69±2.94 bloom). Different treatments caused different damage to the stratum compactum of Pangasius skin.
Keyword: gel, histologi, kolagen, reversibel, suhu

Judul: Effect of NaOH Comparison on Patin Skin Pretreatment (Pangasius hypophthalamus) on Gelatin and Its Application on Hard Capsule Shells
Abstrak: Kulit ikan patin sangat potensial menjadi sumber gelatin, namun proses pretreatment yang dilakukan belum maksimal. Penelitian ini bertujuan menentukan perbandingan NaOH dan sampel dalam proses preatreatment, serta karakteristik fisik-kimia gelatin kulit ikan patin. Metode yang dilakukan yaitu Rancangan Acak Lengkap dan uji lanjut Duncan. Karakteristik gelatin yang telah sesuai dengan standar GMIA 2019 dan SNI 8622-2018. Perlakuan terbaik didapatkan pada perlakuan perbandingan 1:8 (b/v). Perlakuan tersebut menghasilkan rendemen 22,66 ± 1,07%; pH 6,43 ± 0,14; viskositas 67,5 ± 1,50 mps; dan kekuatan gel 244,143 ± 1,69 bloom. Perlakuan tersebut diaplikasikan dalam pembuatan cangkang kapsul keras. Karakteristik cangkang kapsul keras yang dihasilkan telah sesuai dengan standar Departemen Kesehatan RI (2020). Analisis histologi menunjukkan bahwa perlakuan yang digunakan belum mampu menghancurkan lapisan stratum compactum, sehingga perlu dilakukan treatment dengan cara lain agar rendemen gelatin yang dihasilkan maksimal., Pangasius skin has the potential to be a source of gelatin, but the process of pretreatment is not optimal. The purpose of this research was to determine the ratio of NaOH and samples in the process of the pre-treatment, as well as physicochemical characteristics of gelatin from catfish skin. The method used was Completely Randomized Design and Duncan's further test. Gelatin characteristics that are in accordance with the GMIA 2019 standard and SNI 8622-2018. The best treatment was obtained at a 1:8 (w/v) comparison treatment. This treatment resulted in a yield of 22.66 ± 1.07%; pH 6.43 ± 0.14; viscosity 67.5 ± 1.50 mps; and gel strength 244.143 ± 1.69 bloom. This treatment was applied in the manufacture of hard capsule shells. The characteristics of the hard capsule shell produced meet the standards of the Indonesian Ministry of Health (2020). Histological analysis showed that the treatment used had not been able to destroy the layer stratum compactum, so it was necessary to do other treatments in order to maximize the yield of gelatin.
Keyword: catfish, gelatin, hard capsule, NaOH, pretreatment

Judul: Utilization of terra/MODIS L1B data for analysis of horizontal wind profile in the troposphere
Abstrak: This study was concerned with horizontal wind derived from data collected with the Moderate-resolution Imaging Spectroradiometer (MODIS) on board the NASA’s Earth Observation System (EOS)-Terra satellite. The aim of this study is to develop a method for the estimation of horizontal wind movement in the troposphere based on Navier-Stokes equation. The method was applied to a calibrated and geolocated Terra/MODIS L1B 1 km data set. The data inputs to estimate surface temperature are retrieved from TIR (Thermal Infrared) bands 31 (10.780 μm to 11.280 μm) and 32 (11.770 μm to 12.270 μm). The obtained surface temperatures were later used to approximate geopotential height on a constant-pressure surface by using the modified Poisson equation. The main assumptions used are the hydrostatic equilibrium, ideal gas law, constant lapse rate and constant acceleration of gravity. Based on the distribution of geopotential height, the pressure gradient force is measured by the gradient of geopotential on an isobaric coordinate to present the horizontal wind profile. For certain topographic areas especially in the mountain ranges, there was a disadvantage of using pressure as a vertical coordinate which lead to inaccuracy. In spite of that, Terra/MODIS L1B data with 1 km spatial resolution can be used to estimate the horizontal wind movement in the troposphere.
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