Judul: Penentuan Waktu Optimal Pengujian Integritas Membran Plasma Spermatozoa Babi Mengunakan Hypo-osmotic Swelling (HOS) Test
Abstrak: Keutuhan membran plasma merupakan faktor penting untuk menentukan fertilitas spermatozoa. Penelitian ini bertujuan untuk menentukan waktu optimum untuk pengujian membran plasma yang utuh (MPU) pada semen segar babi menggunakan hypo-osmotic swelling (HOS) test. Sebanyak 10 ekor babi dari bangsa Landrace, Duroc dan Yorkshire yang telah dewasa digunakan sebagai sumber semen. Semen dikoleksi menggunakan pemijatan massase. Semen yang diperoleh dievaluasi secara makroskopis dan mikroskopis. Pengujian MPU dilakukan dengan cara memasukkan 50 μL semen ke dalam mikrotub berisi 1 ml larutan hipoosmotik (150 mOsm Kg-1). Campuran larutan diinkubasi pada suhu 37oC. Sperma yang bereaksi dan yang tidak bereaksi terhadap larutan HOS dievaluasi mulai jam ke 0 dan setiap 15 menit dengan total 200 sel sperma. Hasil penelitian menunjukkan bahwa waktu optimal sperma bereaksi maksimal terhadap larutan HOS adalah 60 menit setelah inkubasi.
Keyword: Keutuhan membran plasma, HOS test, semen babi.

Judul: Penentuan Waktu Optimal Pengujian Keutuhan Membran Plasma Spermatozoa Semen Beku Sapi Limousin dan Frisien Holstein Menggunakan Hypo-Osmotic Swelling (HOS) Test.
Abstrak: The study was conducted to determine the optimal time to test the integrity of plasma membrane of Limousin and Frisien Holstein (FH) bull frozen semen spermpatozoa using hypoosmotic swelling (HOS) test. A total of 15 samples from each bull used. All samples were incubated in a hypoosmotic solution with osmolarity of 150 mOsm Kg-1 for 60 minutes. The hypoosmotic solution made by admix 1.351 g fructose, 0.735 Na-citrate and water until the volume was 100 ml. Every 15 minutes the whole sample was evaluated the percentage of spermatozoa that reacts to the solution. Optimal time was obtained at 30’ and 45’, both on Limousin and FH frozen semen. The mean percentage of reacted Limousin bull spermatozoa at 30’ and 45’ respectively were 53.90 ± 6.81 % and 52.47 ± 8.00 %. Whereas the FH bull spermatozoa was 44.52 ± 6.26 % and 44.46 ± 8.42 %. Spermatozoa from frozoen semen needs time to react to hypoosmotic solution due to the added of freezing diluent. This causes the spermaozoa did not react immediately to the hypoosmotic solution. Plasma membrane integrity is very important for sperm fertility. Fertility enzymes contained in the head of spermatozoa, thus if the plasma membrane of the head broken, spermatozoa will lose their fertility. Similarly in the tail, if the plasma membrane broken, spermatozoa will lose their motility.
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Judul: Pelabelan Super Edge Magic pada Graf Cycle dan Graf Wheel
Abstrak: This manuscript proves that cycle graph and wheel graph have a super edge magic labeling. Super edge magic labeling on a graph is labeling that has an edge magic labeling with a set of vertices were mapped in to {1,2,… ,𝑝} and a set of edges were mapped in to {𝑝+1,𝑝+2,… ,𝑝+𝑞}, in which 𝑝 is order and 𝑞 is size on the graph. There are one lemma and two theorems to be discussed. The lemma is used to prove the two theorems. The first theorem proves that cycle graph 𝐶𝑛 is super edge magic if and only if 𝑛 is odd. The second theorem proves that wheel graph 𝑊𝑛 of order 𝑛 is not super edge magic. Moreover 𝑊𝑛 is not edge magic if 𝑛≡0 mod
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