Judul: Karakterisasi enzim kitinase asal isolat 11 UJ
Abstrak: Kitinase merupakan enzim yang menghidrolisis polimer kitin menjadi oligomer kitin atau monomer N-asetilglukosamin. Penelitian ini bertujuan melakukan pemurnian parsial dan karakterisasi enzim kitinase untuk memperoleh data ilmiah aktivitas optimum enzim kitinase asal isolat 11 UJ. Tahapan penelitian dimulai dari isolasi enzim kasar kitinase dan purifikasi parsial dengan pengendapan fraksi amonium sulfat 70% dan dialisis. Aktivitas enzim kitinase ditentukan dengan metode Spindler dan kadar protein enzim kitinase ditentukan dengan metode Bradford. Enzim hasil presipitat amonium sulfat 70% dikarakterisasi untuk memperoleh kondisi optimum enzim baik pH, suhu, waktu inkubasi, pengaruh ion logam terhadap aktivitas enzim, serta penentuan nilai Km dan Vmaks. Pemurnian enzim kitinase dengan amonium sulfat 70% dan dialisis menunjukkan faktor pemurnian masing-masing 2.40 kali dengan yield 2.23% dan 5.23 kali dengan yield 0.83% dibandingkan dengan ekstrak kasar enzim. Hasil karakterisasi menunjukkan bahwa enzim kitinase asal isolat 11 UJ mempunyai pH optimum 8, suhu optimum 37°C, dan waktu inkubasi optimum selama 120 menit. Ion Logam Mn, Fe, Cu, Zn2+, dan EDTA dengan konsentrasi 10 mM diketahui dapat berfungsi sebagai inhibitor bagi enzim kitinase asal isolat 11 UJ. Enzim kitinase mempunyai nilai Km sebesar 0.297 µmol/mL dan Vmaks sebesar 4.68 x 105 µmol/mL detik.
Keyword: Chitinase, Isolates 11 UJ, Partial Purification, Characterization

Judul: Isolasi, Pemurnian Parsial, dan Karakterisasi Enzim Kitinase dari Bacillus sp. BPPT CC 2
Abstrak: Chitinase is a potential enzyme due to its acitvity hydrolyting chitin to its derivates. Therefore, it can be used for various applications, such as agriculture, microbiology, and cosmetic. This study aimed to isolate, purify, and characterize (pH, temperature, metal ion effect, molecular weight, and kinetic parameters) chitinase of Bacillus sp. BPPT CC 2. Ammonium sulphate precipitation and dialysis were used for enzyme purification. Enzyme was characaterized its optimum pH and temperature, metal ions, molecular weight, as well as kinetic parameters. The results showed that enzyme was well precipitated using 80% ammonium sulphate. After dialyzed, level of enzyme purity increased 6.58 fold with specific activity of 0.0924 U/mg. Results showed that the optimum temperature and pH of chitinase were found at 55ºC and pH 5. Value of Km and Vmaks values were 2.705x10-4mg/L second and 0.6959 mg/L. Predicted molecular weight of chitinase was 103 kDa. Addition of metal ions Mn2+and EDTA (5 mM and 10 mM) act as activators of chitinase
Keyword: Bacillus sp. BPPT CC 2, characterize, chitinase, kinetic parameter, purification

Judul: Implementation of Spatio-Temporal Change Query in Relational Database.
Abstrak: Geographic object changes in various views. Object changes are recorded using a model of static storage. No historical connection between old version data and new version data has been built. For that, a storage model of spatio-temporal queries that can analyze spatio-temporal changes has been developed. The object changes the geometry are grouped according to the taxonomy of Spatio-Temporal Pattern Change. This research has been able to apply the query patterns change including born, expand, merge, contract, split, normal and die. This query based on geometric (area) changes. The implementation of spatio-temporal change queries represents an object changes continuously with easy, change detection is done by comparing two versions of data. Keyword: spatio-temporal change query, spatio-temporal change pattern.
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