Judul: Analisis kebutuhan ruang terbuka hijau berdasarkan kebutuhan oksigen dan air di Kota Depok, Propinsi Jawa Barat
Abstrak: Perubahan penggunaan lahan dari kawasan bervegetasi menjadi kawasan terbangun mengakibatkan keseimbangan ekologi kota terganggu, salah satunya terhadap kebutuhan oksigen dan ketersediaan air di Kota Depok. Tujuan penelitian ini adalah menentukan luas ruang terbuka hijau berdasarkan kebutuhan oksigen dan ketersediaan air, serta menilai prefernsi masyarakat terhadap prioritas pengembangan pembangunan di Kota Depok. Penelitian ini menggunakan pendekatan perhitungan Gerarkis dan TIM Fahutan IPB. Metoda Analytic Hierarchy Process (AHP) dipergunakan untuk menentukan faktor-faktor pengembangan pembangunan RTH di Kota Depok. Hasil penelitian menunjukkan bahwa Kota Depok saat ini memiliki ruang terbuka hijau (RTH) seluas 5.125,43 ha, dan berdasarkan perhituangn metoda Gerarkis, RTH untuk tahun 2005 sudah tidak mampu lagi memenuhi kebutuhan oksigen bagi manusia, kendaraan bermotor dan hewan ternak, karena luas RTH di Kota Depok seharusnay 6.155,18 ha.
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Judul: Analisis Ruang Terbuka Hijau dan Kecukupannya di Kota Depok
Abstrak: Greenery open space (GOS) is a vegetated open space in urban areas, which its existence is often sacrificed in city development process. This study aims to determine acreage of GOS changes in Depok, to analyze the adequacy of GOS based on population, to identify level of development of the city, to analyze relationship between GOS changes and level of city development, and to identify factors affecting GOS changes. The results showed that in 2006 the GOS in Depok city was 2359.20 ha and 1729.53 ha in 2011 or it was a decline at about 629,67 ha during the period. Based on population number, Depok required 3.627,23 ha of GOS. Scalogram analysis indicated an increase in hierarchy of some subdistricts to level II and level I, and reducing of level III. The greatest changes of GOS occurred in the lower level of hierarchy (Level-III). This is due to limited land available for construction of facilities that are often sacrificed GOS. Determinants factors of GOS changes consisted of positive determinant, including acreage of vacant land, and negative factors, i.e. area allocated for built up in the regional spatial plan and initial acreage of GOS (2006).
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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