Judul: Tingkah Laku Domba Garut Jantan Muda dengan Pemeliharaan Intensif yang Diberi Ransum Limbah Tauge pada Waktu Pemberian yang Berbeda
Abstrak: Research aimed to evaluate the effect of bean sprout waste as grass substitution and feeding time toward the ingestive, agonistic, and resting behaviour of the lamb. About 16 heads of lamb were maintained for 3 months with 2 treatments factors (feed and feeding time). The method was focal sampling and the datas were analysed using ANOVA. The result showed that there was no interaction between feed and feeding time toward the ingestive, agonistic, and resting behaviour. Feeding time gave significant effect (P<0.05) to the ingestive frequencies and agonistic duration. Morning feeding time gave higher consumption than afternoon feeding time meanwhile, afternoon feeding time gave higher effect for agonistic behaviour than morning feeding time.
Keyword: resting, ingestive, feeding time, feed, agonistic

Judul: Tingkah Laku Harian dan Pola Makan pada Domba Garut dengan Pemberian Pellet yang Mengandung Limbah Tauge dan Legum Indigofera sp.
Abstrak: Changes in the feed management can contribute to the change of behaviour that will impact on the productivity of livestock. The aim of this research was to study sheep behaviour as a result of giving bean sprouts waste pellet and Indigofera sp. pellet in the ration. This research used 6 garut lambs of 3 month old. There were two types experimental rations: (1) 30% of Indigofera sp.+70% concentrate and (2) 30% of bean sprout waste+70% concentrate. Parameter measured were sheep daily behaviour and eating behaviour. The data were recorded by a one zero sampling method and analyzed by Chi-square test. Data temperature and humidity obtained during the research were analyzed by Analysis of Varian (ANOVA). The result of temperature in the noon was significant higher than in the morning and afternoon, humidity in the morning was significant higher than in the noon and afternoon. Results showed that the daily behaviour exhibited by sheep with two types of ration (bean sprout waste and Indigofera sp.) were not significant. The activity of feed checking behaviour was significant, where the provision of Indigofera sp. pellet had greater frequency than bean sprout waste pellet, but did not cause stress to livestock. It is concluded that the two type of rations can be recommended to use in local sheep farm
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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