Judul: Antimicrobial Activity of Bacillus sp. Lts 40 against Ralstonia solanacearum, Pseudomonas syringae pv. glycines, and Pseudomonas fluorescens.
Abstrak: The using of chemical substance as biocontrol could leave the residue which acumulated on environment. Therefore, it is necessary to have more safety and non residual substances. Determination of antimicrobial activity of Bacillus sp. Lts 40 isolated from shirmp pond was conducted in vitro experiments. This isolate was assayed their inhibition activity to the growth of six isolates (Escherichia coli, Staphylococcus aureus, Pseudomonas fluorescens A, Pseudomonas fluorescens B, Ralstonia solanacearum, and Pseudomonas syringae pv. glycines) of plant pathogenic bacteria using spread plate method. The results showed that the isolate was able to inhibit the growth of all indicator bacteria. The higher inhibiting activity was found on Ralstonia solanacearum.
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Judul: Antimicrobial Activity of Bacillus sp. to Xanthomonas oryzae pv. oryzae, Pseudomonas syringae pv. glycines, and Pseudomonas fluorescens.
Abstrak: Leaf blight disease caused by bacterial plant pathogen such as X. oryzae pv. oryzae and P. syringae pv. glycines had deathly effect to plants. The pathogens can be controlled by biological control using P. fluorescens or antimicrobial substances. The research studied the inhibition ability and production of antimicrobial substances produced by Bacillus sp. againts bacterial plant pathogen such as X. oryzae pv. oryzae, P. syringae pv. glycines, and biological control P. fluorescens. As many as 31 Bacillus sp. isolates isolated from forest soil was determined their antimicrobial activity against indicator bacteria using double layer method. The result showed that 13 isolates was able to inhibit Staphylococcus aureus, Eschericia coli, and X. oryzae pv. oryzae growth, 8 isolates could inhibit P. fluorescens A growth, 9 isolates could inhibit P. fluorescens B growth, and 8 isolates could inhibit P. syringae pv. glycines growth. Bacillus sp. F13 had the fastest inhibition and good efficiency against all indicator bacteria. Mixed culture competition test showed that this isolate had a broad spectrum inhibition activity, it was able to inhibit Gram positive and Gram negative bacteria. The isolate could inhibit growth of P. syringae pv. glycines and X. oryzae pv. oryzae up to 100%. The antimicrobial substance the isolate was produced in the late logarithmic growth phase.
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Judul: Millennial and Zoomer Generation Consumers’ Purchasing Decisions and Preferences at Coffee Shops in Madiun City
Abstrak: Perkembangan zaman melahirkan generasi baru dengan preferensi minuman kopi yang diduga berbeda dari generasi sebelumnya. Ketersediaan pilihan kedai kopi telah memperluas alternatif yang dapat dipertimbangkan oleh konsumen dalam keputusan pembelian. Sebagai salah satu kedai kopi, IKIO Coffee dapat melakukan segmentasi pasar berdasarkan karakteristik tertentu. Penelitian ini bertujuan untuk mengidentifikasi perbedaan karakteristik konsumen generasi Millennial dan Zoomer serta melakukan segmentasi konsumen berdasarkan karakteristik demografis, perilaku, dan psikografis dalam pembelian minuman kopi di kedai-kedai kopi di Kota Madiun. Data primer dikumpulkan melalui kuesioner yang diisi oleh 180 responden pada periode April - Juli 2022. Metode pengambilan sampel menggunakan teknik judgmental sampling dengan kriteria kelompok usia. Analisis yang dilakukan meliputi analisis deskriptif, uji T sampel bebas untuk analisis perbedaan, dan analisis cluster menggunakan metode K-Means Clustering. Hasil penelitian menunjukkan bahwa konsumen generasi Millennial dan Zoomer cenderung didominasi oleh perempuan, angkatan kerja, memiliki tujuan mengisi waktu luang, mencari informasi melalui media sosial, dan memberikan prioritas pada cita rasa. Terdapat perbedaan signifikan antara konsumen Millennial dan Zoomer dalam preferensi kedai kopi terkait media promosi, fasilitas WIFI/stopkontak, fasilitas hiburan live music, proses pemesanan, dan proses pembayaran. Berdasarkan preferensi terhadap atribut kedai kopi, segmentasi konsumen terbagi menjadi tiga segmen, yaitu pecinta kopi, petualang rasa kopi, dan konsumen yang mengedepankan kualitas minuman kopi. Implikasi manajerial bagi IKIO Coffee dan kedai-kedai kopi lainnya adalah mengarahkan strategi pemasaran lebih khusus pada segmen konsumen petualang rasa untuk generasi Zoomer, serta mengutamakan inovasi dan diferensiasi dalam menyajikan minuman kopi berkualitas untuk generasi Millennial.
Keyword: coffee shops, consumer preferences, generation Millennial, generation Zoomer, segmentation