Judul: Evaluasi Pengelolaan Lanskap Kawasan Wisata Goa Lawa Purbalingga sebagai Ekowisata Berkembang
Abstrak: Proses pengembangan kawasan wisata Goa Lawa dilakukan untuk meningkatkan minat wisatawan. Tujuan penelitian ini adalah melakukan inventarisasi, penilaian, serta memberikan evaluasi dan rekomendasi dari manajemen program wisata kawasan agar dapat meningkatkan keuntungan baik sosial maupun ekonomi bagi masyarakat di sekitar dengan tidak mengurangi fungsi ekologis dan budaya lokal dari Goa Lawa. Tiga analisis yang dilakukan di antaranya analisis kualitatif melalui wawancara, studi pustaka, dan metode inventarisasi Starke dan Simonds, analisis kuantitatif dengan metode Recreation Opportunity Spectrum (ROS) dan Uji Kruskal Wallis, serta analisis kesenjangan untuk membandingkan kondisi aktual dan ideal dari sebuah objek wisata. Tahap rekomendasi yang dilakukan dibagi menjadi dua yaitu rekomendasi aktivitas wisata dan rekomendasi fasilitas wisata. Hasil yang didapat menunjukkan bahwa Kawasan Wisata Goa Lawa merupakan Kawasan wisata rural developed, dalam uji Kruskal Wallis tidak ada perbedaan nyata diantara persepsi keenam objek dari tiga kelompok responden.
Keyword: Analisis kesenjangan, Goa Lawa, Recreation Opportunity Spectrum

Judul: Evaluasi Pengelolaan Lanskap Kawasan Wisata Badega Gunung Parang di Purwakarta Sebagai Ekowisata Berkelanjutan
Abstrak: Proses pengembangan yang dilakukan pada kawasan wisata Badega Gunung Parang untuk meningkatkan atensi wisatawan dan melindungi alam sekitar agar berkelanjutan. Tujuan dari penelitian ini adalah melakukan inventarisasi, penilaian, dan memberikan evaluasi serta rekomendasi terhadap manajemen program wisata agar dapat menambahkan keuntungan baik sosial maupun ekonomi untuk masyarakat sekitar tanpa mengurangi budaya serta fungsi ekologis Gunung Parang. Analisis yang dilakukan diantaranya analisis kualitatif dengan studi pustaka, metode analisis inventarisasi Starke and Simonds, analisis kuantitatif dengan metode Recreation Opportunity Spectrum dan Uji Kruskal Wallis. Membandingkan keadaan lapang dan idealnya dari sebuah objek wisata untuk analisis kesenjangan. Bagian rekomendasi yang diberikan berupa rekomendasi strategi pengelolaan kawasan wisata, rekomendasi aktivitas wisata, rekomendasi pembagian ruang wisata, dan rekomendasi fasilitas wisata. Berdasarkan hasil yang didapat kawasan wisata Badega Gunung Parang mencirikan bahwa kawasan wisata Badega Gunung Parang merupakan kawasan wisata rural developed. Uji Kruskal Wallis menunjukkan adanya perbedaan yang nyata diantara persepsi kelompok responden terhadap keenam objek., The development process carried out in the Badega Gunung Parang tourist area is to increase the attention of tourists and protect the surrounding nature so that it is sustainable. The purpose of this study is to conduct an inventory, assessment, and provide evaluations and recommendations for the management of tourism programs in order to add social and economic benefits to the surrounding community without compromising the culture and ecological function of Mount Parang. The analyzes carried out include qualitative analysis with literature studies, the Starke and Simonds inventory analysis method, quantitative analysis using the Recreation Opportunity Spectrum method and the Kruskal Wallis test. Comparing the field conditions and ideals of a tourist attraction for gap analysis. The recommendation section given is in the form of recommendations for tourism area management strategies, recommendations for tourist activities, recommendations for sharing tourist spaces, and recommendations for tourist facilities. Based on the results obtained, the Badega Gunung Parang tourist area characterizes that the Badega Gunung Parang tourist area is a developed rural tourism area. The Kruskal Wallis test shows that there is a significant difference between the perceptions of the respondent's group of the six objects.
Keyword: Gap analysis, Mount Parang, Recreation Opportunity Spectrum, Sustainable

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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