Judul: Pengaruh kelompok teman sebaya dan media massa terhadap keterampilan sosial atlet muda di SMA Negeri Ragunan Jakarta
Abstrak: Pengaruh teman sebaya dan media massa semakin meningkat pada usia remaja menyebabkan remaja harus memiliki keterampilan sosial yang baik. Penelitian ini bertujuan untuk menganalisis pengaruh teman sebaya dan media massa terhadap keterampilan sosial atlet muda di SMA Negeri Ragunan Jakarta. Metode penelitian menggunakan cross-sectional study dengan desain penelitian cluster random sampling. Jumlah contoh penelitian ini adalah 85 orang yang merupakan siswa kelas XI SMA Negeri Ragunan Jakarta. Hasil penelitian menunjukkan bahwa terdapat hubungan usia ibu, jumlah teman sebaya di sekolah, kualitas hubungan pertemanan dengan teman sebaya, dan pemanfaatan media massa dan keterampilan sosial. Penelitian ini juga menunjukkan bahwa usia ibu, status orangtua, kualitas hubungan pertemanan dengan teman sebaya, dan pemanfaatan media massa terhadap keterampilan sosial
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Judul: Interaction Parent and Perr, Social Media Usuge, and Social Skill
Abstrak: Keterampilan sosial merupakan kemampuan yang perlu dimiliki oleh seorang remaja untuk membentuk sikap empati dan kesadaran sosial. Tujuan penelitian ini untuk menganalisis interaksi remaja – orang tua dan teman sebaya, serta pengunaan media sosial terhadap keterampilan sosial. Penelitian ini melibatkan 100 remaja akhir usia 18 sampai 21 tahun di seluruh Indonesia. Penelitian ini menggunakan desain Cross-sectional, metode survey dengan alat bantu kuesioner menggunakan google form. Kualitas interaksi remaja – orang tua dan teman sebaya, penggunaan media sosial, serta keterampilan sosial berada dalam katagori sedang. Hasil uji kolerasi menunjukkan bahwa terdapat hubungan positif interaksi remaja – orang tua dan teman sebaya serta penggunaan media sosial dengan keterampilan sosial. Hasil uji regresi menunjukkan terdapat pengaruh positif interaksi remaja – teman sebaya dan penggunaan media sosial terhadap keterampilan sosial. Artinya, semakin baik kualitas interaksi remaja dengan teman sebaya dan penggunaan media sosial, akan meningkatkan keterampilan sosial., Social skills are abilities that need to be possessed by a teenager to form an attitude of empathy, social awareness, and mutual understanding. The purpose of this study was to analyze the interaction of adolescents - parents and peers, and the use of social media on social skills. This study involved 100 late teens throughout Indonesia. This study uses cross-sectional, using survey and questionnaire methods with google form. The sample has a moderate category on the quality of interaction between adolescents - parents and peers, use of social media, and social skills. The results of the correlation test show that there is a positive relationship between adolescent-parent and peer interactions on the use of social media. In addition, adolescent-parent and peer interactions, and use of social media were positively related to social skills. The results of the regression test showed that there was a positive effect of adolescent – peer interaction and the use of social media on social skills.
Keyword: adolescent, interaction, social media, social skill

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable