Judul: Landscape Services Management of Water Resource and Thermal Comfort of City Park in Jakarta
Abstrak: Jakarta terus mengalami peningkatan kepadatan kota yang menyebabkan Ruang Terbuka Hijau (RTH) berkurang. Dampak yang terjadi adalah penurunan kualitas lanskap dan kenyamanan hidup karena adanya keterbatasan resapan air dan peningkatan suhu. Hal tersebut dibuktikan oleh Indeks Kualitas Lingkungan Hidup Jakarta 2022 yang berada pada kategori sedang. Taman kota mampu memberikan kontribusi yang signifikan sebagai penyedia jasa lanskap dalam menjaga keseimbangan siklus air dan mengatur iklim mikro. Penelitian ini bertujuan menganalisis jasa lanskap pengaturan tata air dan kenyamanan termal pada Taman Bambu, Taman Cattleya dan Taman Langsat, lalu menyusun rekomendasi pengelolaan lanskapnya. Perangkat i-Tree Eco digunakan untuk analisis jasa lanskap tata air menggunakan plot sampel. Temperature Humidity Index (THI) dilakukan untuk mengetahui kategori kenyamanan melalui pengukuran suhu dan kelembapan. Penyusunan rekomendasi strategi pengelolaan jasa lanskap dilakukan dengan analisis Strengths, Weaknesses, Opportunities, and Threats (SWOT). Hasil analisis i-Tree Eco menunjukkan pohon di ketiga taman kota mampu mengurangi limpasan 51,28 m³/tahun dengan nilai yang diperkirakan Rp1.944.573,61. Hasil analisis THI menunjukkan Taman Bambu memiliki THI 30, Taman Cattleya memiliki THI 30,4, dan Taman Langsat memiliki THI 30,2, ketiganya menunjukkan kategori kenyamanan yang tidak nyaman. Berdasarkan hasil analisis SWOT, ketiga taman kota berada pada Kuadran V, yakni pengelolaanya harus disusun berdasarkan hold and maintain strategy., Jakarta continues to experience increasing urban density, leading to a reduction in Green Open Spaces (GOS). This has decreased landscape quality and living comfort due to limited water infiltration and rising temperatures, evidenced by Jakarta's 2022 Environmental Quality Index, which is categorized as moderate. City parks play a significant role in provisioning landscape services by maintaining water cycles and regulating microclimates. This study aims to analyze the landscape services related to water resource and thermal comfort in Taman Bambu, Taman Cattleya, and Taman Langsat, and formulate recommendations for its landscape management. The i-Tree Eco was used to analyze water resource service using sample plots. The Temperature Humidity Index (THI) was used to determine the comfort category through temperature and humidity measurements. Recommendations for landscape management strategies were formulated using Strengths, Weaknesses, Opportunities, and Threats (SWOT) analysis. The i-Tree Eco analysis results show that trees in the three city parks can reduce runoff by 51,28 m³/year, with an estimated value of IDR 1.944.573,61. The THI analysis results show that Taman Bambu has a THI of 30, Taman Cattleya has a THI of 30,4, and Taman Langsat has a THI of 30,2, all indicating an uncomfortable category. Based on the SWOT analysis results, the three city parks are in Quadrant V, suggesting that their management should be developed based on a hold and maintain strategy.
Keyword: I-Tree Eco, jasa lanskap, manajemen lanskap, taman kota, temperature humidity index

Judul: Management of Water Resources Landscape Services and Comfort in Urban Forests of Jakarta City
Abstrak: Meningkatnya jumlah penduduk di Kota Jakarta mengakibatkan peralihan fungsi lahan vegetasi menjadi lahan terbangun. Adanya peralihan fungsi tersebut dapat berpengaruh terhadap kemampuan dalam menyerap air dan tingkat kenyamanan hidup di perkotaan. Permasalahan lingkungan yang ada di Kota Jakarta dapat diperbaiki dengan keberadaan ruang terbuka hijau (RTH) salah satunya hutan kota (HK) yang merupakan hamparan lahan yang didalamnya berisi pohon-pohon yang kompak dan rapat. Penelitian ini berfokus kepada HK Munjul dan HK Srengseng untuk mengidentifikasi mengenai karakteristik hutan, menganalisis jasa lanskap tata guna air dan kenyamanan termal, serta menyusun rekomendasi pengelolaan jasa lanskap HK. Pengambilan data dilakukan langsung dengan mengukur dan mengamati pohon di setiap plot sampel dan mengukur suhu dan kelembapan pada kedua HK. Analisis dilakukan dengan menggunakan I-Tree Eco untuk pengelolaan tata air dan Temperature Humidity Index (THI) untuk menentukan tingkat kenyamanan dengan mengukur nilai suhu dan kelembapan udara yang ada di HK Munjul dan HK Srengseng. Berdasarkan analisis menggunakan I-Tree Eco, nilai serapan air yang dapat diserap di masing-masing HK sebesar 4.049,86 m3 /th dan 13.751,65 m3 /th. Limpasan air yang dapat dikurangi oleh kedua HK sekitar 159.000 m3 /th dengan nilai sekitar Rp150.000.000. Berdasarkan analisis THI, kedua HK memiliki tingkat kenyamanan yang cukup nyaman dengan nilai THI masing-masing 27,0 dan 25,2. Berdasarkan hasil analisis Strengths Weaknesses Opportunities Threats (SWOT), HK Munjul dan HK Srengseng berada pada Kuadran V (Hold and Maintain Strategy). Strategi-strategi yang dapat diimplementasikan di kedua HK seperti pengembangan infrastruktur yang memadai dan penawaran kegiatan wisata, melakukan pengawasan dan pemantauan secara teratur terhadap kegiatan pengelolaan dan pemeliharaan, serta meningkatkan kegiatan pengelolaan dan pemeliharaan dalam meminimalkan kerusakan.
Keyword: Urban Forest, Landscape Services, Management, Blue Open Space, Landscape Comfort

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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