Judul: Gaya Pengasuhan Ibu, Kelekatan dengan Teman Sebaya, dan Konsep Diri Remaja pada Keluarga Ibu Bekerja dan Tidak Bekerja
Abstrak: The aims of this study was to analyze the influence of parenting style of mother and peer attachment on adolescent’s self-concept. Sample of this research were the families with working and nonworking mother had teenager child which is aged 12-15 years who lived in Kebon Pedes and Kedung Badak Village, Tanah Sareal, Bogor City. There were 100 families selected randomly consisting of 50 working mother families and 50 nonworking mother families that were interviewed by questionnaire. The result of corellation test showed there were significant relationships between gender, age of parents and adolescents, family’s income, authoritative parenting, authoritarian parenting, permissive parenting, and peer attachment with adolescent’s self concept. Based on regression test, it was found that variables influenced adolescent’s self concept were adolescent’s allowance, families’s socio-economic, authoritative parenting style, and peer attachment.
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Judul: Komunikasi dan Kelekatan Orang Tua, Kelekatan Teman Sebaya, serta Kepuasan Hidup Remaja berdasarkan Status Bekerja Ibu
Abstrak: This study aimed to analyze the influences of parent communication and attachment to peer attachment and adolescent life satisfaction. The samples of this study is a family with teenagers aged 12-15 years, as many as 100 families were selected randomly from two villages in Tanah Sareal, Bogor City. Data was collected through interview using questionnaires. The results showed there was significant different a type of positive communication was according father perception, peer attachment as alienation dimension, adolescent life satisfaction as global, self, and school dimensions. The results of correlation test showed there was significant relationship between parent’s communication, parent and peer attachment with adolescent life satisfaction. The based of regression test, the factor’s which the influences of peer attachment that female, employment mother’s, family incomes, and mother’s communication, where as the factor’s which the influences of adolescence life satisfaction that was employment mother’s, father’s communication, and peer attachment.
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Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.