Judul: Penerapan Scrum dengan Game-Based Retrospective: Starfish dalam Pengembangan Aplikasi Zikirin
Abstrak: Tim pengembang aplikasi Zikirin menggunakan metode manajemen pengembangan perangkat lunak Scrum, tetapi masih menggunakan metode sprint retrospective dengan menjawab pertanyaan “Apa yang sudah berjalan dengan baik?” dan “Apa yang harus di-improve?” melalui platform Notion. Metode sprint retrospective ini tidak bisa menjelaskan aktivitas-aktivitas apa saja yang perlu dipertahankan, diperbanyak, dikurangi, dimulai, dan dihindari tim pengembang aplikasi Zikirin pada sprint berikutnya. Pada sprint keenam, peneliti berhasil menerapkan game-based retrospective: Starfish kepada tim pengembang aplikasi Zikirin secara daring melalui perangkat lunak kolaboratif Figma dan Zoom untuk melihat evaluasi sprint yang telah dilakukan oleh tim pengembang aplikasi Zikirin secara lebih rinci dan detail. Metode game-based retrospective: Starfish yang diterapkan pada sprint keenam ini dapat mengetahui aktivitas-aktivitas yang perlu dipertahankan, diperbanyak, dikurangi, dimulai, dan dihindari pada sprint selanjutnya selama proses pengembangan aplikasi Zikirin., The Zikirin application development team utilizes the Scrum software development management method, but still uses the sprint retrospective method by answering the questions "What went well?" and "What needs improvement?" through the Notion platform. This sprint retrospective method cannot explain the specific activities that need to be maintained, increased, decreased, started, and avoided by the Zikirin application development team in the next sprint. In the sixth sprint, the researcher successfully implemented a game-based retrospective: Starfish to the Zikirin application development team through the collaborative software Figma and Zoom to obtain a more detailed evaluation of the sprint conducted by the Zikirin application development team. The game-based retrospective method: Starfish implemented in this sixth sprint can identify the activities that need to be maintained, increased, decreased, started, and avoided in the next sprint during the Zikirin application development process.
Keyword: perangkat lunak, retrospeksi, Scrum, Zikirin

Judul: Analysis of Scrum Implementation Results and Strategy Formulation in the Development of Micro Productive Loan Products at PT XYZ
Abstrak: Scrum sebagai kerangka kerja Agile, diterapkan untuk meningkatkan efisiensi dan efektivitas pengembangan perangkat lunak. Namun, implementasi Scrum di PT XYZ menghadapi beberapa tantangan. Melalui observasi partisipan selama delapan sprint, penelitian ini mengidentifikasi faktor-faktor yang memengaruhi kinerja tim. Analisis SWOT digunakan untuk mengevaluasi kekuatan, kelemahan, peluang, dan ancaman dalam implementasi Scrum. Hasil analisis menunjukkan bahwa pembagian story points yang seimbang, manajemen dependensi sesama tim, dan penyesuaian beban kerja adalah kunci untuk meningkatkan kinerja tim. Strategi yang disusun berdasarkan analisis SWOT mencakup langkah-langkah konkret untuk memperbaiki implementasi Scrum, seperti redistribusi tugas, peningkatan komunikasi, dan pelatihan tambahan bagi anggota tim. Penelitian ini memberikan kontribusi praktis bagi PT XYZ dalam mengoptimalkan penerapan Scrum untuk pengembangan produk pinjaman produktif mikro., Scrum as an Agile framework is applied to improve the efficiency and effectiveness of software development. However, the implementation of Scrum at PT XYZ faces several challenges. Through participant observation over eight sprints, this study identifies factors affecting team performance. SWOT analysis is used to evaluate the strengths, weaknesses, opportunities, and threats in Scrum implementation. The results of the analysis indicate that balanced story points allocation, dependency management between teams, and workload adjustment are key to improving team performance. The strategy formulated based on the SWOT analysis includes concrete steps to enhance Scrum implementation, such as task redistribution, improved communication, and additional training for team members. This study provides practical contributions to PT XYZ in optimizing Scrum application for the development of micro productive loan products.
Keyword: analisis swot, Pengembangan produk, scrum, kinerja tim, pinjaman produktif mikro

Judul: Characterization and Expression of Tomato infectious chlorosis virus Coat Protein Gene in Escherichia coli
Abstrak: Tomato infectious chlorosis virus (TICV) is a member of Crinivirus genus. TICV infects tomato crop in Garut and Cipanas, West Java Indonesia. Tomato initially showed bright interveinal yellowing symptoms. The coat protein gene (CP-TICV) was amplified by RT-PCR from total RNA extracted from infected tomato leaves and the amplified fragment was cloned and completely sequenced. The fragment was subsequently subcloned into the pET-21b expression vector. The recombinant plasmid was transformed to Escherichia coli strain BL21(DE3)pLysS to express the coat protein. The coat protein fused to a 6xhistag, was purified by affinity chromatography using a NiNTA spin column. The identity of the purified protein was confirmed by SDS-PAGE. In this experiment, 792 bp of CP-TICV gene of TICV virus has been successfully cloned, sequenced, and expressed in E. coli. Based on nucleotide sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain strain and 99% identity to North America, France, and California strains and based on amino acid sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain, North America, France strains and 99.2% identity to California strain. CP-TICV showed over expressed in E. coli when it is induced with 1 mM IPTG and incubated at 37oC. Purified CP-TICV-Histag recombinants protein sized 29 kDa based on SDSPAGE analysis.
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