Judul: Karakterisasi Ekson dan Intron 7, 8, 9 Gen Endo-β-1,4-glukanase pada Rayap Coptotermes curvignathus
Abstrak: Rayap mampu mendegradasi selulosa karena adanya endo-β-1,4-glukanase rayap. Rayap Coptotermes curvignathus adalah spesies rayap yang banyak ditemukan di Indonesia. Rayap ini merupakan hama penting dengan kemampuan mencerna kayu yang tinggi. Gen endo-β-1,4-glukanase rayap C. curvignathus (CcEG) belum memiliki data genomik lengkap. Dengan demikian, penelitian ini bertujuan untuk mengkarakterisasi ekson dan intron 7, 8, 9 gen endo-β-1,4-glukanase pada rayap C. curvignathus (CcEG). Ekstraksi DNA menggunakan metode CTAB yang dimodifikasi. Alignment nukleotida dan asam amino dilakukan antara endo-β-1,4-glukanase rayap C. curvignathus (CcEG) hasil penelitian ini dengan endo-β-1,4-glukanase rayap C. formosanus (CfEG) dan Nasutitermes takagoensis (NtEG). Ekson 7, 8, 9 dan intron 7, 8 CcEG masing-masing berukuran 165, 140, 214, 503 dan 546 pb. Ekson 7, 8, dan 9 CcEG panjangnya sama dengan ekson yang sama pada gen endo-β-1,4-glukanase rayap C. formosanus (CfEG) dan homolog 99%. Hasil deduksi ekson 7, 8, 9 CcEG sebanyak 172 asam amino putative dan masuk dalam kelompok Glycosyl Hydrolase Family 9 (GHF 9). Intron 7 dan 8 CcEG berbeda panjang dan posisi dengan intron 8, 9 gen endo-β-1,4-glukanase rayap Nasutitermes takasagoensis (NtEG). Hasil penelitian ini dapat menjadi dasar untuk penelitian mengenai karakterisasi enzim selulase rayap C. curvignathus.
Keyword: Isoptera, Rhinotermitidae, cellulase enzyme, Glycosyl Hydrolase Family 9 (GHF)

Judul: Karakterisasi gen endo-b-1,4-glukanase pada rayap Coptotermes curvignathus
Abstrak: Termites are social insects that have a distinct task in form of caste within their colony. Workers play an important role in finding and degrading the cellulose food, due to the existence of endo- -1,4-glucanase enzyme. Hence, this study was aimed to characterize Coptotermes curvignathus endoglucanase gene. Primers were designed based on the Coptotermes formosanus (CfEG) cDNA and Nasutitermes takasagoensis (NtEG) genomic DNA. These introns of C. curvignathus all showed GT/AG rule. Exon two up to five of C. curvignathus endoglucanase were aligned with exon three up to six from NtEG; while the intron two to four of C. curvignathus endoglucanase were at the same position with that of intron three up to five on NtEG. Exon two up to five of C. curvignathus endoglucanase resulted 564 bp and BLASTN analysis revealed that C. curvignathus endoglucanase homologous to CfEG. Putative amino acid obtained from C. curvignathus endoglucanase showed its high homology with CfEG. Analysis based on Pfam revealed that C. curvignathus endoglucanase was member of Glycosyl Hydrolase Family 9 (GHF 9) family and six-hairpin glycosidase superfamily. Motifs found in endoglucanase C. curvignathus were one consensus signature of GHF 9, nucleophile, co-nucleophile, salt bridge, and N-terminal. Based on DNA and amino acid homology between CfEG and endoglukanase C. curvignathus it was expected that endoglukanase C. curvignathus has similar high cellulase activity as shown in CfEG. This will be an essential database for finding an effective cellulase enzyme for further applications.
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Judul: Dampak Perceraian dan Hubungan Sosial terhadap Kesejahteraan Psikologis Ibu sebagai Orang Tua Tunggal
Abstrak: Family with a single mothers who came from divorced, often bring the impact such as problem or change on a single mothers well-being. The aim of this research to indentify characteristics of single mother and relations with psychological well-being and social relations, and also analyze impact divorce on psychological well-being and social relations. Descriptive analysis, regression, and Pearson correlation was used in this study. The respondents were sixty single mother who come from the length divorced more than six month. The result showed that compare with present condition, psychological well-being, impact of divorce and social relation respondent are more significant lower than the first six month after divorce. There was relationship between age of present and divorce, family’s income, and size of family with level of depression, social support, and economic impact. Based on regression analysis we found there was negative effect of impact of divorce to psychological well-being at the first six month after divorce and the present. Other wise, we found there was not significant effect of divorce to social relationship.
Keyword: economic impact, level of depression, social support