Judul: Pendugaan komponen periodik fungsi intensitas berbentuk  fungsi periodik kali tren linear suatu proses poisson  non-homogen
Abstrak: In this manuscript, estimation of the periodic component of an intensity function with a form of periodic function multiplied by a linear trend of a non homogeneous Poisson process is discussed. The estimator is constructed using a single realization of a Poisson process observed in the interval It is assumed that the period of the periodic component is known. The convergence of the mean square error (MSE) of the estimator has been proved. In addition, asymptotic approximations to the bias, variance, and mean square error of the estimator have been established. An asymptotic optimal bandwidth has also been found.
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Judul: Pendugaan komponen periodik fungsi intensitas berbentuk fungsi periodik kali tren kuadratik suatu proses Poisson non-homogen
Abstrak: This manuscript is concerned with estimation of periodic component of the intensity function of form periodic function multiplied by quadratic trend of a non-homogeneous Poisson process. It is considered the worst case, where there is only a single realization of a Poisson process with intensity function of form periodic component multiplied by a quadratic trend were observed in the interval [0,n]. It is assumed that the period of the periodic component is known. An estimator of the periodic component of the intensity function has been formulated and its Mean Square Error (MSE) has been proved converges to zero as n   . In addition, asymptotic approximations to the bias, variance, and the Mean Square Error (MSE) of the estimator have been formulated. An asymptotically optimal bandwidth for this estimator is also determined.
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Judul: Gene Editing  of Two Cyanide Synthesis-related Genes in Cassava using CRISPR/Cas9
Abstrak: Cassava (Manihot esculenta) contain toxic quantities of cyanogenic glucoside. Reducing the potentially toxic cyanogenic glucoside levels in cassava could be achieved by gene editing through Clustered Regularly Interspaced Short Palindromic Repeats – CRISPR associated protein 9 (CRISPR/Cas9), a high- precision genome editing technique in plants. Two cassava UDP- glucosyltransferase (UGT) paralogs, UGT85K4 and UGT85K5, are the genes catalyzing the synthesis of cyanogenic glucosides in plants. The study aimed to construct the CRISPR/Cas9 recombinant vector carrying single guide RNA (sgRNA) to edit UGT85K4 and UGT85K5 genes, and transform sgRNA CRISPR/Cas9 construct into KU50 cassava calli. Five sgRNAs were designed, considering the on/off target efficiency score and GC content. Those sgRNAs were ligated into pRGEB32 plasmid and introduced to competent Escherichia coli DH5a. The PCR colony and DNA sequencing were performed to verify the sgRNA insert. The amplicons showed the expected band size of recombinant vector (180 bp). Based on sequencing results, only one sgRNA was successfully ligated into the plasmid that will be used to edit the UGT85K5 gene. The sgRNA CRISPR/Cas9 construct in pRGEB32 plasmid was successfully transformed into Agrobacterium tumefaciens and introduced into KU50 cassava calli through co-cultivation. The overgrowth of Agrobacterium occurred after the co-cultivation phase.
Keyword: CRISPR/Cas9, Cyanogenic glucoside, Gene editing, sgRNA