Judul: Trees Carbon Reserve Estimation Of City Greenery Open Space (GOS) In East Jakarta Municipality Using Landsat Imagery
Abstrak: In the end of this decade, earth temperature raised up to 0.6 ºC. The main factor that is considered as the driver of global warming is the increasing of green house gasses concentration in atmosphere, i.e carbondioxide (CO2), methane (CH4) and Nitrogenoxide (N2O). During this last decade CO2 emission increated twice from 1400 million ton/year to 2900 million ton/year. Some researches showed that CO2 concentration in atmosphere in 1998 is about 330 ppm the increament 1.5 ppm per year (Brown et al, 1996), and has probability to keep increasing year by year. Based on all the problem mentioned above, this research was conducted to estimate carbon reserve on trees stands of Greenery Open Spaces at East Jakarta Municipality using Landsat satelite temporal imagery (few years). The best model concluded is Y = 43,448E + 11G-3,69MIRI-2,28 (R2 = 42.8%). The total area of East Jakarta Municipality is about 18.689,64 hectare with trees carbon reserve above ground soil for 1986 is about 184.975 ton/hectare (LANDSAT MSS imagery 1986), 1992 is about 165.050 ton/hectare (LANDSAT 5 imagery 1992), 2001 is about 181.805 ton/hectare (LANDSAT 7 SLCOff imagery 2001) and 2005 is about 183.710 ton/hectare (LANDSAT 7 ETM+ imagery).
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Judul: Penggunaan Penginderaan Jauh dan SIG untuk Mengetahui Perubahan Penutupan Lahan dan Kecukupan Ruang Terbuka Hijau sebagai Rosot Karbondioksida (Studi Kasus; Kota Bogor Tahun 1991, 2000, dan 2012)
Abstrak: Bogor city is a residential as well as services city. The city have been experiencing land cover change, from nature to variety of infrastructure purpose. As a result, it will increase the air pollution especially CO2 in the atmosphere. One way that can be used to reduce level of air pollution is development of green open space in spatial planning in the city of Bogor. The results of the analysis of spatial and temporal Landsat imagery Bogor City area in 1991, 2000, and 2012 showed this land cover in general is dominated by sparse vegetation amounted to 3.221,09 hectares or 28,01%, 4.009,90 hectares or 34,86%, 4.114,36 hectares or 27,07%. Land cover changes during the two periods was decrease of agricultural land, sparse vegetation, shrubs, and open land. Meanwhile land cover the increase vegetation. The need for green space in the city of Bogor area could be determined by the approach of CO2 absorption. Based on the level of CO2 emissions in the city of Bogor need 29.770,25 hectares open space, the available open space only 4.040,28 hectares or 35,13%. The Bogor city need addition green open space approximately 25.729,97 hectares. Value of CO2 emissions in 2025 amounted to 61.103,38 Gg, so the vast green space required is 1.048.848,17 hectares of area assuming the actual circumstances in the field using image data 2012.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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