Judul: Perhitungan Aktuaria untuk Manfaat Pensiun-Normal Menggunakan Metode Projected Unit Credit dan Entry Age Normal.
Abstrak: Retno
Keyword: projected unit credit, pension funding method, normal cost, entry age normal, actuarial liability

Judul: Optimizing Normal Pension Fund Financing Using the Actuarial Valuations Method with Variation of Assumptions
Abstrak: Program Dana Pensiun merupakan suatu upaya untuk mengantisipasi risiko pada masa pensiun dengan memberikan manfaat bagi karyawan yang pensiun. Penelitian ini membahas penghitungan aktuaria untuk program pensiun manfaat pasti dengan menggunakan metode Unit Credit (UC), Projected Unit Credit (PUC), Entry Age Normal (EAN), dan Attained Age Normal (AAN). Tujuan penelitian ini adalah melakukan penghitungan aktuaria yang mencakup penghitungan iuran normal dan kewajiban aktuaria serta menentukan metode yang optimal bagi peserta dan perusahaan berdasarkan pada akumulasi nilai iuran normal dengan tiga asumsi. Asumsi pertama adalah kondisi Indonesia 2020 yaitu suku bunga 10,24% dan kenaikan gaji 2%, asumsi kedua adalah suku bunga 8,34% dan kenaikan gaji 2% serta asumsi ketiga adalah suku bunga 6,06% dan kenaikan gaji 3%. Berdasarkan pada hasil penghitungan untuk masing-masing asumsi, metode EAN adalah metode yang optimal bagi peserta sedangkan metode UC adalah metode yang optimal bagi perusahaan., The pension fund program is an attempt to anticipate risks in retirement period by providing benefit the retired employees. This research discusses actuarial calculations for defined benefit pension plans using the Unit Credit (UC), Projected Unit Credit (PUC), Entry Age Normal (EAN), and Attained Age Normal (AAN) methods. The research aims to perform actuarial calculation including the calculation of normal cost and actuarial liability, and to determine the optimal method for participants and companies based on the accumulation of normal cost with three assumptions. The first assumption is the condition of Indonesia in 2020 with an interest rate of 10,24% and a salary increase of 2%, the second assumption is an interest rate of 8,34% and a salary increase of 2% and the third assumption is an interest rate of 6,06% and a salary increase of 3%. Based on the calculations for each assumption, the EAN method is the optimal method for the participants while the UC method is the optimal method for the companies.
Keyword: Actuarial Cost Method, Attained Age Normal, Entry Age Normal, Projected Unit Credit, Unit Credit

Judul: DNA Extraction and Characterization of Salmonella‟s Lytic Bacteriophage Genome
Abstrak: Bacteriophages are ubiquitous in nature and are known to proliferate wherever their bacterial hosts exist. Their virion particles can exist independently outside the host, however all phages are need their host to propagate. Several phages are highly specific to host cell surface receptors and any slight changes in structure results in little or no interaction between the phage and its host. Salmonella‟s phage isolated from sewage water where their bacteria exist. Three isolated of phages (phages 15, 19 and 38) were used in this research that concentrated using three types of methods. PEG/ NaCl method was firstly used to concentrate the phages based on their molecular weight to precipitate the phage particles during centrifugation. The second method was Scraping Plaque method. In this method, overlay plaques were scraped and collected in SM buffer to get phage concentrates. Enrichment method was the last method used to concentrate the phages. Phage genome isolation was done using two methods i.e. EDTA/SDS and Proteinase K methods. The first method was done using DNase I to eliminate bacterial DNA followed by SDS/EDTA treatment to release phages DNA and phenol-chloroform-isoamyl alcohol to extract the DNA. DNA was collected by isopropanol extraction. While in Proteinase K method, bacterial DNA was eliminated by DNase I and Proteinase K/SDS was used to degrade phages capsids and ethanol for DNA precipitation. The result showed that PEG/NaCl method resulted the highest phage concentration for phage 15 and phage 19, while scrapping method was the highest for phage 38. The quantity and purity of extracted DNA were low for PEG/NaCl and Scraping methods, while Enrichment method was higher indicated higher purity. Run on agarose gel electrophoresis of extracted DNA confirmed that Enrichment method results were the best while scraping and PEG/NaCl method was appeared smear background. Based on DNA genome size determination, the bacteriphages of Salmonella sp were comparable with other study of Salmonella phages. Phage P15 with genome size of 39,5 kb was close to phage epsilon15. Phage P19 with genome size of 41 kb was close to phage ST160. And phage P38 with genome size of 48,5 kb was close to phage Gifsy-1.
Keyword: