Judul: Effectiveness of Stem Cell Therapy in Myocardial Infarction a Review of Values Creatinine, Ureum and Kidney Histopathology
Abstrak: Infark miokardium merupakan suatu kondisi terbentuknya nekrosis miokardium ditandai dengan penurunan cardiac output yang memicu terjadinya acute kidney injury. Terapi stem cell adalah metode yang dapat memperbaiki infark miokardium. Penelitian ini bertujuan untuk mempelajari efektivias terapi stem cell placenta tunggal maupun kombinasi kardiomiosit pada babi yang mengalami infark miokardium ditinjau dari nilai ureum, kreatinin dan histopatologi organ ginjal. Sebanyak 9 ekor babi di bagi menjadi tiga kelompok, yaitu tanpa terapi (K1), terapi stem cell placenta tunggal (K2), dan terapi kombinasi kokultur kardiomiosit (K3). Seluruh babi dilakukan ligasi arteri circumflexa coronary untuk mensimulasikan iskemia jantung dan setelah 1 jam ligasi dilakukan pemberian terapi. Hasil penelitian menunjukkan penurunan nilai ureum dan kreatinin pada K2 dan K3. Hasil histopatologi ditemukan kongesti atrofi glomerulus, dan nekrosis tubulus. Berdasarkan uji analysis of variance (ANOVA) menunjukkan ada perbedaaan yang nyata (p < 0,05). Disimpulkan bahwa pemberian terapi stem cell placenta maupun yang dikombinasi dengan kokultur kardiomiosit dapat menurunkan nilai ureum, kreatinin, dan memperbaiki jaringan ginjal secara histopatologi. Kata kunci: infark miokardium, ginjal, histopatologi, ureum, kreatinin, stem cell, ABSTRACT Myocardial infarction is a condition where myocardial necrosis develops which is characterized by a decrease in cardiac output that triggers acute kidney injury. Stem cell therapy is a method that can repair myocardial infarction. This study aims to study the effectiveness of placental stem cell therapy alone or in combination with cardiomyocytes in pigs with myocardial infarction in terms of urea, creatinine and histopathological values of the kidneys. A total of 9 pigs were divided into three groups, namely without therapy (K1), single placenta stem cell therapy (K2), and combined therapy with cardiomyocyte coculture (K3). All pigs underwent coronary circumflex artery ligation to simulate cardiac ischemia and after 1 hour of ligation, therapy was given. The results showed an decreased in urea and creatinine values in the K2 and K3 groups. Histopathological results were congestion, glomerular atrophy, and tubular necrosis. Based on the analysis of variance (ANOVA) test, it showed that there was a significant difference (p<0.05). It was concluded that administration of placental stem cell therapy or in combination with cardiomyocyte coculture can reduce urea, creatinine,and improve kidney tissue histopathologically. Keywords: myocardial infarction, kidney, histopathology, urea, creatinine, stem cell
Keyword: stem cell, kidney, myocardial infarction, ureum, creatinine

Judul: Ultrasonography Characteristics of Swine Heart Myocardial Infarction with pAEC and Cardiomyocyte Stem Cells Theraphy
Abstrak: Kerusakan miokardium yang disebabkan infark miokardium serta kematian kardiomiosit menyebabkan penurunan fungsi dan kinerja jantung yang dapat dievaluasi dengan ekokardiografi. Terapi sel punca pada jantung merupakan satu metode yang dapat meregenerasikan dan memperbaiki kerusakan kardiomiosit. Tujuan penelitian ini adalah untuk menilai karakteristik jantung babi yang mengalami infark miokardium serta mengamati perubahan morfometri dan kinerja jantung yang diterapi sel punca porcine Amniotic Ephitelial Cell (pAEC) dengan dan tanpa kombinasi kardiomiosit melalui ekokardiografi. Hewan model babi (n=15) dilakukan operasi induksi pembuatan infark miokardium (IM). Terapi sel punca diberikan kepada dua kelompok pAEC dan pAEC + kardiomiosit, babi dilakukan perawatan hingga 56 hari kemudian dieutanasia. Ekokardiografi dilakukan sebelum induksi IM untuk mendapatkan nilai normal, satu jam setelah induksi IM dan 56 hari setelah terapi sel punca. Hasil ekokardiografi normal jantung babi untuk parameter detak jantung, left ventricular internal dimension diastole (LVIDd), dan stroke volume menunjukkan hasil yang berbeda antara babi jantan dan betina. Pasca induksi infark miokardium, kelompok pemberian terapi pAEC + kardiomiosit menunjukkan peningkatan kinerja jantung pada variabel nilai rata-rata detak jantung, stroke volume, ejection fraction dan fractional shorthening. Kelompok terapi pAEC tunggal menunjukkan peningkatan hanya pada variabel cardiac output. Terapi sel punca pAEC + kardiomiosit meningkatkan kinerja jantung dengan mendukung promosi sel kadiomiosit jantung., Myocardial injury caused by myocardial infarction and cardiomyocyte loss leads to depress cardiac function and performance that can be evaluated by echocardiography. Cardiac stem cell therapy is one of the method that can regenerate and repair damaged cardiomyocytes. The aim of this study was to evaluate the characteristics of the swine heart that had myocardial infarction and to observe the morphometric changes and cardiac performance treated with porcine Amniotic Ephithelial Cell (pAEC) stem cells with and without cardiomyocyte combination through echocardiography. Swine animal models (n=15) were subjected to surgical induction of myocardial infarction (MI). Stem cell therapy was given to two groups of pAEC and pAEC + cardiomyocytes, swine were treated for up to 56 days and then euthanized. Echocardiography was measured before IM induction to obtain normal echocardiographic values, one hour after IM induction and 56 days after stem cell therapy. Normal echocardiography results of the swine heart for parameters of heart rate, left ventricular internal dimension diastole (LVIDd), and stroke volume showed different results between male and female swine. After myocardial infarction surgery, the pAEC + cardiomyocyte therapy group showed improved cardiac performance in the variables of mean heart rate, stroke volume, ejection fraction and fractional shortening. The single pAEC therapy group showed improvement only in the variable of cardiac output. PAEC + cardiomyocyte stem cell therapy improves cardiac performance by promoting cardiac cadiomyocyte cells.
Keyword: swine, echocardiography, myocardial infarction, cardiomyocytes, pAEC

Judul: Asymptotic Distribution of Estimator for the Intensity Function of a Doubly Periodic Poisson Process
Abstrak: In this manuscript, estimation of the intensity function of a doubly periodic Poisson process is discussed. It is assumed that only a single realization of the Poisson process is observed in a bounded window [0,n]. It is also assumed that the period of the intensity function is known. The rate of consistency and asymptotic normality of the estimator are formulated. Finally, a confidence interval for the local intensity function is also formulated.
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