Judul: Pengembangan Backend Forum Tani pada Digital Tani dengan Metode Prototyping
Abstrak: Indonesia adalah negara agraris yang sebagian besar masyarakatnya bermata pencaharian sebagai petani. Kurangnya edukasi yang merata mengenai pertanian mengakibatkan sulitnya pemahaman tentang permasalahan baru yang dihadapi petani. Adanya forum online dapat membantu petani dalam mendiskusi permasalahan baru dengan lingkup yang lebih luas. Tani Center IPB mengembangkan aplikasi Digital Tani sebagai one stop solution untuk masalah pertanian Indonesia. Penelitian ini bertujuan untuk mengembangkan backend forum tani pada aplikasi Digital Tani dengan metode prototyping. Sistem dikembangkan dengan menggunakan framework Laravel dengan arsitektur Model, View, Controller (MVC). Hasil pengembangan diuji menggunakan blackbox testing dan telah berhasil memenuhi Minimum Viable Product (MVP) dengan iterasi yang dilakukan sebanyak 2 kali., Indonesia is an agricultural country where most of the people make a living as farmers. The lack of equitable education about agriculture makes it difficult to understand the new problems faced by farmers. The existence of online forums can help farmers in discussing new problems with a wider scope. Tani Center IPB developed the Digital Tani application as a one stop solution for Indonesian agricultural problems. This study aims to develop a farmer forum backend on the Digital Tani application using the prototyping method. The system was developed using the Laravel framework with Model, View, Controller (MVC) architecture. The results of the development were tested using blackbox testing and had successfully met the Minimum Viable Product (MVP) with 2 iterations.
Keyword: agriculture, backend, forum, MVC, prototyping method

Judul: Pengembangan Modul Frontend Forum Tani Pada Aplikasi Web Digital Tani Menggunakan Metode Prototyping
Abstrak: Pertanian merupakan salah satu komoditas andalan di Indonesia, kendati demikian masalah dapat muncul ketika petani tengah melakukan pekerjaannya. Berdasarkan studi literatur, forum diskusi online dinilai sebagai wadah yang baik sebagai tempat berdiskusi bagi petani. IPB telah mengembangkan aplikasi Digital Tani yang merupakan aplikasi untuk membantu menyelesaikan masalah-masalah pertanian. Penelitian ini akan mengembangkan modul forum tani pada aplikasi web Digital Tani dari sisi frontend menggunakan metode Prototyping. Hasil dari penelitian ini merupakan modul yang memiliki fitur utama forum seperti membuat, melihat, dan melaporkan thread dan juga fitur manajemen forum oleh admin. Hasil pengujian pada iterasi terakhir menggunakan metode blackbox testing menunjukkan semua fitur pada modul forum tani sudah berjalan dengan baik. Modul forum tani diharapkan dapat menjadi wadah bagi petani untuk melakukan diskusi seputar pertanian dengan sesama petani atau dengan para pakar dan penyuluh yang ada di IPB melalui aplikasi web Digital Tani., Agriculture is one of the mainstay commodities in Indonesia, however problems can arise when farmers are doing their jobs. Based on a literature study, online discussion forum are considered to be a good forum for discussions for farmers. IPB has developed the Digital Tani application which is an application to help solve agricultural problems. This research will develop forum module on the Digital Tani web application from the frontend side using the Prototyping method. The results of this study are modules that have the main forum features such as creating, viewing, and reporting threads as well as forum management features by the admin. The test results in the last iteration using the blackbox testing method show that all the features on the forum module have been running well. The forum module is expected to be a place for farmers to have discussions about agriculture with fellow farmers or with experts and instructor at IPB through the Digital Tani web application.
Keyword: Digital Tani, forum, frontend, agriculture, Prototyping

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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