Judul: Hubungan Kekerabatan Beberapa Isolat Lentinus Asal Tropis Berdasarkan Sifat Mikroskopik Kultur dan Molekular
Abstrak: Jamur Lentinus merupakan jamur pelapuk kayu yang berpotensi sebagai obat yang tersebar di berbagai kawasan daerah tropis dan subtropis. Penelitian ini bertujuan untuk mengetahui hubungan kekerabatan jamur Lentinus isolat tropis berdasarkan sifat mikroskopik kultur dan sifat molekular. Sampel yang digunakan ialah Lentinus isolat LU3, LU10 (L. torulosus), LC4, LC6 (L. cladopus), LSC1, LSC7 (L. Sajor-caju), LP9 (L. quarrosulus), dan HS serta JTP (Pleurotus). Media yang digunakan adalah agar ekstrak malt pepton (AEMP) dan agar ekstrak malt (AEM) pada suhu 35°C untuk isolat Lentinus dan ±29°C untuk isolat HS dan JTP. Struktur mikroskopik kultur setiap isolat diamati setelah isolat berumur 2 dan 4 minggu. Analisis molekular dilakukan dengan mengamplifikasi DNA berdasarkan metode PCR-RAPD dengan menggunakan 2 primer acak tunggal 10-mer, yaitu RP1 dan RP3. Sifat mikroskopik kultur dan molekular dinyatakan ke dalam data biner dan diolah dengan program MEGA 4.1. Hasil pengamatan menunjukkan bahwa beberapa isolat Lentinus yang ditumbuhkan pada media AEMP memiliki penampakan koloni yang berbeda dengan penampakan koloni pada media AEM. Berdasarkan pohon kekerabatan mikroskopik kultur dan molekular, isolat Pleurotus (JTP) berada di luar kelompok A (kelompok Lentinus dan HS) sebagai out group. Kelompok A terbagi menjadi dua cabang yaitu HS dan kelompok Lentinus (B, C, D). Berdasarkan sifat molekular kelompok Lentinus terdiri dari kelompok B dengan nilai konsistensi (NK) 57% yang terdiri dari LP9 dan LSC1 (NK=89%) dan kelompok C dengan NK 20%. Kelompok C terdiri dari LSC7 dan LC6 (NK=65%) dan kelompok D dengan NK=45%. Kelompok D terdiri dari isolat LU10 dan isolat LC4 dan LU3 (NK=46%). Hubungan kekerabatan Lentinus berdasarkan molekular belum spesifik sehingga perlu dianalisis kembali dengan penambahan primer lain.
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Judul: Hubungan Kekerabatan Beberapa isolat  Lentinus  Asal Tropis  Berdasarkan Sifat Mikroskopik Kultur dan Molekular
Abstrak: Lentinus mushroom is a wood rot fungi used as medicine and distributed in tropic and subtropic regions. The research aimed to know the phylogeny relationship of tropical Lentinus mushroom isolates based on culture microscopic and molecular characters. Isolates of Lentinus that were used are LU3, LU10 (L. torulosus), LC4, LC6 (L. cladopus), LSC1, LSC7 (L. sajor- caju), and LP9 (L. squarrosulus), HS, and JTP (Pleurotus). Culture media were malt extract peptone agar (MEPA) and malt extract agar (MEA) at 35°C for Lentinus isolates and ±29°C for HS and JTP isolates. The culture microscopic structure of each isolate was observed at 2 and 4 weeks old of cultures. Molecular analysis carried out amplification of DNA with PCR-RAPD method using two 10-mer single random primers, RP1 and RP3. The culture microscopic and molecular characters were converted into biner data and analysis using MEGA 4.1 program. The observation showed that the colony appearances of some Lentinus isolates on MEPA media were different from those of MEA. Based on phylogenic tree of culture microscopic and molecular characters, Pleurotus isolate (JTP) was separated from A group (Lentinus and HS) as out group. A group was devided to two branches, HS isolate and Lentinus group (B, C, D). Based on molecular analysis, Lentinus group included B group(consistency value, CV=57%) which devided LP9 and LSC1 isolates (CV=89%) and C group (CV=20%). C Group were devided into LSC7 and LC6 (CV=65%) and D group (CV=45%). D group included LU10 isolate and LC4 and LU3 isolates (CV=46%). Molecular relationship of Lentinus based on molecular analysis was not yet specific so need to analyze detailed with added another primer.
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Judul: Numerical modeling of the hydrodynamics of standing wave and scouring in front of impermeable breakwater with different steepnesses
Abstrak: The aim of this paper is to numerically study the effects of breakwater steepness on the hydrodynamics of standing wave and scouring process in front of impermeable breakwaters. A two-dimensional hydrodynamics model based on the Reynolds Averaged Navier–Stokes (RANS) equations and the Volume of Fluid (VOF) method was developed and then combined with an empirical sediment transport model. Comparisons with an analytical solution and experimental data showed the present model is very accurate in predicting the near bottom velocity and capable of simulating the scour/deposition patterns consistent with experimental data. It was found that the additional terms of bottom shear stress in the momentum equations are necessary to produce a physical scouring pattern. Different breakwater steepnesses produce different characteristics of standing wave, the steady streaming system, and scouring pattern in front of the breakwaters, which also affects the correlations between them. An additional analysis of the turbulence field parameters and the sediment transport rate was also performed. All these important information will be presented in details in this paper and can be worthwhile for designing the breakwater in coastal areas.
Keyword: The RANS–VOF model Standingwave Steadystreamingsystem Scouring pattern Breakwatersteepness