Judul: Pengaruh Nilai Dan Kelompok Acuan Terhadap Pembelian Hedonis Konsumen Muda
Abstrak: Pembelian hedonis merupakan suatu pembelian yang berdasarkan pada aspek kesenangan, melibatkan sensori dan diluar kebutuhan. Tujuan dari penelitian ini adalah menganalisis pengaruh nilai dan kelompok acuan terhadap pembelian hedonis konsumen muda. Desain penelitian ini menggunakan cross sectional study dengan metode survei secara self report menggunakan kuisioner. Hasil penelitian didukung dengan wawancara mendalam terhadap beberapa contoh. Penelitian ini melibatkan 205 orang mahasiswa Strata-1 (S1) Institut Pertanian Bogor yang dipilih secara proportional random sampling. Analisis statistik yang digunakan adalah uji beda independent t-test dan analisis linear berganda. Terdapat perbedaan signifikan pada dimensi nilai kegembiraan dan pembelian hedonis antara laki-laki dan perempuan. Nilai berpengaruh secara signifikan dan positif terhadap pembelian hedonis, sedangkan kelompok acuan tidak berpengaruh signifikan terhadap pembelian hedonis.
Keyword: kelompok acuan, nilai, pembelian hedonis

Judul: Studi Komparasi Nilai, Kontrol Diri, Sikap, dan Pembelian Impulsif Antargenerasi
Abstrak: Saat ini perkembangan pasar semakin kompleks, namun apabila hal ini tidak diimbangi dengan kemampuan konsumen untuk mengelola pembeliannya dapat menyebabkan munculnya pembelian impulsif. Penelitian ini bertujuan untuk membandingkan dan menganalisis pengaruh nilai, kontrol diri, dan sikap terhadap pembelian impulsif generasi Baby Boomer, generasi X, dan generasi Y. Penelitian menggunakan desain studi kuantitatif dan cross sectional study, pengambilan data dilakukan dengan wawancara menggunakan kuesioner. Lokasi penelitian berada di Wiguna Tengah Regency, Wiguna Timur Regency, Wiguna Selatan Regency, dan Taman Rivera, Surabaya. Penelitian dilakukan pada bulan Februari 2019. Responden adalah ibu rumah tangga sebanyak 90 orang yang terbagi dalam tiga generasi dan dibagi dengan nonprobability sampling secara quota sampling. Hasil penelitian menunjukkan bahwa pada variabel kontrol diri, sikap, dan pembelian impulsif terdapat perbedaan yang signifikan. Selain itu, variabel kontrol diri, ditemukan memengaruhi sikap dan pembelian impulsif secara signifikan. Kontrol diri berpengaruh positif terhadap sikap dan berpengaruh negatif terhadap pembelian impulsif.
Keyword: generasi baby boomer, generasi X, generasi Y, kontrol diri, nilai, pembelian impulsif, sikap

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable