Judul: Analisis Keragaman Intron 2 Partial dan Ekson 3 Gen KIT pada Sapi Bali Menggunakan Metode Direct Sequencing
Abstrak: Sapi bali merupakan sapi asli Indonesia dengan warna rambut jantan dewasa hitam dan betina kemerahan. Abnormalitas warna rambut pada sapi bali seperti pola white spotted dan albino diduga disebabkan salah satunya oleh gen KIT. Penelitian ini bertujuan untuk menganalisis keragaman gen KIT intron 2 partial dan ekson 3 dan hubungannya dengan warna rambut pada sapi bali. Penelitian dilakukan di Laboratorium Genetika Molekuler, FAPET IPB. Sampel darah sapi bali sebanyak 48 sampel terdiri 15 sampel white spotted, 13 albino, dan 20 normal. SampeldiperolehdariBPTU-HPTSerdangDenpasarBali,danLombok. Analisis keragaman gen KIT menggunakan metode direct sequencing dengan panjang sekuen target 448 bp. Data hasil sekuen gen KIT dianalisis menggunakan program MEGA7.0.26 dan BioEdit72.50. Hasil analisis menunjukkan fragmen ekson 3 gen KIT pada sapi bali monomorfik, namun ditemukan SNP g.70208534A>G di fragmen intron 2 bersifat polimorfik tetapi SNP tidak dapat digunakan sebagai marka abnormalitas warna rambut normal. Simpulan, keragaman ekson 3 gen KIT pada sapi bali bersifat tidak beragam atau monomorfik, namun pada intron 2 partial ditemukan SNP g.70208534A>G beragam atau polimorfik.
Keyword: gen KIT, sapi bali, SNP, warna rambut

Judul: Exploration of Partial Intron 1 and Exon 2 Diversity of the KIT Gene in Bali Cattle Using the Direct Sequencing Method
Abstrak: Bali cattle (Bos Javanicus) are native Indonesian cattle. There are hair color abnormalities in bali cattle such as white spotted and albino hair color patterns. This study aims to identify the diversity of the KIT gene in the partial intron 1 and exon 2 regions and to analyze its relationship with hair color in bali cattle. The research was conducted at the Molecular Genetics Laboratory, Faculty of Animal Science, IPB. The number of samples used were 48 blood samples of bali cattle including: 15 blood samples of bali cattle with white spotted hair, 14 albino and 20 samples of normal hair color. Research samples were obtained from BPTU-HPT Serdang, Denpasar, Bali and Lombok. Analysis of diversity used the direct sequencing method and the sequence results were analyzed using the MEGA7.0.26 and BioEdit72.50 programs. The target sequence of the KIT gene is 234 bp. Based on the results of the analysis, the KIT gene in bali cattle is monomorphic so that this fragment cannot be used as a differentiator for normal hair color, white spotted and albino in bali cattle. Thus, it was concluded that the diversity of partial intron 1 and exon 2 regions of the KIT gene in bali cattle is not diverse or monomorphic.
Keyword: bali cattle, hair color, KIT gene, SNP

Judul: Hydroxyapatite-Chitosan Composite Coating on Stainless Steel 316 to Improve Corrosion Resistance
Abstrak: Metals that were used in bone implantation should have good corrosion resistance that were not easily corroded when implanted into the body. Metal corrosion resistance of stainless steel 316 can be enhanced through the metal coating by hydroxyapatite-chitosan composite with composition ratio of hydroxyapatite (gram) and chitosan 3% (mL) 0.2:0, 0.2:0.1, 0.2:0.5, 0.2:1.0, and 0.2:1.5. Besides improving the corrosion resistance of metal, the presence of chitosan also maintain the position of hydroxyapatite on metal surfaces. Corrosion resistance increases with increasing amount of chitosan added and the best composition of hydroxyapatite-chitosan composite was 0.2:1.0.
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