Judul: Kesesuaian Lahan Pinus merkusii Jungh et de Vriese pada Areal Bekas Tectona grandis Linn. f. (Studi Kasus di RPH Wanareja KPH Banyumas Barat)
Abstrak: West Banyumas Forest Management Unit (WBFMU) is one of the forest management units in working area of Perum Perhutani Unit I Central Java, which has a Corporate Class (CC) of pine trees. Recently, the WBFMU intend to expand the acreage of pine stand in order to increase pine resin production. To achieve this purpose, WBFMU use the former logged over area of teak to be planted by pine. But, the problem raises because the planted pine seedlings showing unappropiate growth. So that, this research was aimed to determine the suitability of pine and limiting factors affecting pine growth in teak stands area at WBFMU. The results show that the suitability of land in the area of the former teak stands in plot 22 B is suitable (S) and plot 25 C is not suitable (N) for the development of P. merkusii according to bonita.
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Judul: Pemetaan Tegakan Pinus merkusii di KPH Banyumas Barat dan Sekitarnya Menggunakan Citra Landsat 8.
Abstrak: Pinus merkusii merupakan jenis tanaman pionir, memiliki tingkat pertumbuhan yang tinggi dan memiliki banyak kegunaan. KPH Banyumas Barat merupakan salah satu perusahaan yang memanfaatan pinus sebagai sumber pendapatan perusahaan, sehingga diperlukan pemetaan tegakan pinus. Tujuan penelitian ini adalah memetakan sebaran hutan pinus dan mengetahui informasi potensi pinus hutan rakyat di sekitar KPH Banyumas Barat menggunakan citra Landsat 8. Metode yang digunakan adalah klasifikasi terbimbing (supervised classification). Klasifikasi dilakukan menggunakan metode kemiripan maksimum (maximum likelihood). Jenis tutupan lahan yang dapat diidentifikasi yaitu awan, bayangan awan, badan air, pemukiman, sawah, mangrove, tegakan non pinus, tegakan pinus Perhutani, dan tegakan pinus hutan rakyat. Hasil pemetaan menunjukkan luas pinus Perhutani 21 760.58 ha dan pinus hutan rakyat 6 916.56 ha, dengan akurasi kappa (kappa accuracy) sebesar 92.01%. Pinus Perhutani memiliki potensi tegakan sebesar 338.04 m³/ha. Pinus hutan rakyat memiliki potensi tegakan sebesar 244.55 m³/ha. Potensi produksi getah pinus di KPH Banyumas yaitu mencapai 13 462.09 ton/tahun dan potensi produksi getah pinus hutan rakyat mencapai 5 011.96 ton/tahun.
Keyword: klasifikasi terbimbing, pemetaan, Pinus merkusii, potensi tegakan tutupan lahan

Judul: Excretory/secretory antigen of ascaridia galli L3 stage to trigger yolk immunoglobulin in laying hens
Abstrak: Ascaridiosis caused by the nematode Ascaridia galli. A study was carried out to characterize excretory/secretory antigen of A. galli from larval stage to trigger anti-ascaridiosis yolk immunoglobulin in laying hens. A. galli L3 were recovered from intestines of 100 heads chickens 7 days after oesophagus inoculation with 6000 L2. L3 recovered in this manner were cultured (5 – 10 ml-1) in flasks containing rosswell park memorial institute (RPMI) 1640 media, pH 6.8, without phenol red. Cultures were incubated at 370C in 5% CO2 and culture fluid was collected after 3 days in culture. To prepare excretory/secretory antigen, culture medium were concentrated with vivaspin 30.000 MWCO, and antigen protein concentration were counted as described in Bradford method. The molecular weight of excretory/secretory antigen was determined with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). Active immunizations were applied intra muscularly of chickens with an initial dose of 80 μg. The immunizations were repeated three times with dose of each 60 μg with an interval of one week. The first immunizations were excretory/secretory antigen mixed with fruend adjuvant complete and subsequently mixed with freund adjuvant incomplete. The chicken’s eggs were collected from 49 day after immunizations. Anti-ascaridiosis yolk immunoglobulin (IgY) was extracted from egg yolks by means of ammonium sulphate and purified using fast performance liquid chromatography (FPLC). The purity of anti-ascaridiosis IgY was determined by Bradford method (λ = 280 nm). Antibody was detected by agar gel precipitation test (AGPT) and enzyme linked immunosorbant assay (ELISA). Antigen were detected with immunohistochemistry. Chickens were challanged with dose of 1000 L2 A. galli. Passive immunizations were applied ten times with dose of each 0,875 mg IgY with an interval of one day intra orally. IgY anti-ascaridiosis was tested against A. galli survival in vitro.
Keyword: Unggas, Isolasi dan karakterisasi antigen, Purifikasi imunoglobulin yolk, Pengukuran antibodi ayam petelur, Metode Imunohistokimia, Ascaridiosis