Judul: Urban Forest Priority Development Based on Air Pollution Distribution and Land Temperature in Kota Jambi
Abstrak: Kota Jambi terus mengalami perkembangan dan berimplikasi pada pesatnya pembangunan. Hal tersebut dapat menyebabkan penurunan kualitas lingkungan sehingga dibutuhkan hutan kota dalam memitigasinya. Penelitian ini bertujuan untuk menentukan potensi lokasi hutan kota berdasarkan sebaran polutan udara dan suhu permukaan di Kota Jambi. Penentuan lokasi prioritas hutan kota menggunakan sebaran polutan udara dan suhu permukaan yang dikelaskan menurut kriteria lokasi prioritas, sedangkan tutupan lahan menjadi acuan dalam penentuan lokasinya. Data dikumpulkan menggunakan citra satelit Sentinel-5P berupa CO, NO₂, O₃ dan SO₂, sedangkan nilai suhu permukaan dan tutupan lahan dari citra Landsat 8. Semua data diolah di Google Earth Engine. Potensi lokasi hutan kota dikategorikan menjadi tiga kelas prioritas yaitu prioritas 1 dengan nilai polutan dan suhu permukaan tertinggi meliputi Kecamatan Danau Teluk, Jambi Timur dan Pelayangan; Prioritas 2 dengan nilai polutan dan suhu permukaan menengah meliputi Kecamatan Danau Sipin, Jelutung, Paal Merah dan Pasar Jambi; serta prioritas 3 dengan nilai polutan dan suhu permukaan terendah., Jambi City continues to prosper and has implicated to its rapid development. It can cause a decrease in urban environmental quality, hence the existence of urban forests is needed to mitigate such problems. This study aims to determine the potential locations for urban forests based on the distribution of air pollutants and surface temperature in Kota Jambi. Determination of priority locations uses distribution of air pollutants and surface temperature map that are classified according to priority location criteria, while land cover becomes a reference in determining its location. Sentinel-5P satellite is used for collecting CO, NO₂, O₃, and SO₂ whereas the Landsat 8 satellite is used for collecting surface temperature and land cover. The data are processed on Google Earth Engine. The potential locations for urban forests in Jambi City categorized into three priority classes, namely priority 1 with the highest pollutant and surface temperatures values which are the Danau Teluk, Jambi Timur and Pelayangan District; Priority 2 with medium pollutant and surface temperatures values includes of the Danau Sipin, Jelutung, Paal Merah, and Pasar Jambi Districts; and Priority 3 with the lowest pollutant and surface temperature value.
Keyword: Air pollution, surface temperature, urban forest

Judul: Potensi Pengembangan Hutan Kota Berdasarkan Distribusi Suhu Udara di Kota Palu
Abstrak: Pertumbuhan penduduk dan ekonomi mendorong peningkatan kebutuhan lahan di Kota Palu. Hal ini berdampak pada alih fungsi lahan bervegetasi menjadi lahan non vegetasi dan peningkatan suhu. Hutan kota adalah salah satu jenis ruang terbuka hijau yang dapat berperan dalam meredam suhu perkotaan.Tujuan penelitian ini adalah menentukan prioritas dan potensi lokasi pengembangan hutan kota berdasarkan distribusi suhu udara di Kota Palu. Citra Landat 8 OLI/TIRS dianalisis untuk mengetahui suhu udara, tutupan lahan dan nilai NDVI. Semua data diolah di perangkat lunak Arcmap versi 10.5. Prioritas dan potensi lokasi pengembangan hutan kota ditentukan melalui analisis tumpang susun data suhu udara, tutupan lahan, nilai NDVI dan RTRW Kota Palu. Prioritas pengembangan hutan kota terbagi ke dalam tiga kelas prioritas yaitu prioritas 1 seluas 6740,86 ha, prioritas 2 seluas 2743,78 ha dan priotas 3 seluas 20.695,04 ha. Prioritas 1 merupakan area-area yang paling direkomendasikan untuk dikembangkan menjadi hutan kota sebagaimana 98,31% dari luasnya bersuhu ?27,1 ?., Population and economic growth have driven an increase in land demand in Palu City. This causes the conversion of vegetated land to non-vegetated land and increasing temperatures. Urban forests are a type of green open space that can play a role in reducing urban temperatures. The aim of this research is to determine priorities and potential locations for urban forest development based on the distribution of air temperatures in Palu City. Landat 8 OLI/TIRS imagery was analyzed to determine air temperature, land cover and NDVI values. All data were processed in Arcmap software version 10.5. Priority and potential locations for urban forest development are determined through overlap analysis of the gathered data on air temperature, land cover, NDVI and Urban Land Use Plan of Palu City. Urban forest development priorities are divided into three priority classes, namely priority 1 covering an area of 6740.86 ha, priority 2 covering an area of 2743.78 ha and priority 3 covering an area of 20.695.04 ha. Priority 1 is the most recommended areas to be developed into urban forests as 98.31% of the area has a temperature of ?27.1 ?., ,
Keyword: Hutan Kota, Urban forest, Suhu udara, Air Temperature

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.