Judul: Transformasi Genetik Kentang (Solanum tuberosum L.) Kultivar Agria dengan Gen Pembungaan Hd3a
Abstrak: Potato is an important vegetables commodity in Indonesia. Agria has a potential cultivar to be used as french fries material. Flowering induction of this cultivar is very important to use this cultivar as a parent in the crossing with other cultivar. The aim of this research was to transform genetically potato cultivar Agria with Hd3a flowering gene controlled by rolC promoter. Potato plants were propagated in vitro on MS2 macro medium. Genetic transformation was carried out by co-cultivation method by using Agrobacterium tumefaciens. Putative transgenic calli and shorts were selected in MS and MS2 macro media respectively containing 10-20 mg/L hygromycin. The results showed that the transformation efficiency was 5.01% with regeneration efficiency relatively high 100%. Putative transgenic shoot regeneration per calli was one. Putative transgenic plants produced tuber in MS2 macro media whereas all non-transgenic plants did not. This result indicated that Hd3a induced formation of tuber of putative transgenic potato cultivar Agria.
Keyword: Bogor Agricultural University (IPB), Solanum tuberosum L., Hd3a flowering gene, genetic transformation, cultivar Agria

Judul: Transformasi Genetik Kentang (Solanum tuberosum L.) Kultivar Baraka dengan Gen Pembungaan Hd3a
Abstrak: Flowering has a very important role in plant breeding. Flowering is the phase transition from the vegetative to the generative phase. One of the genes associated with flowering is Hd3a. This research has an objective to transform genetically potato (Solanum tuberosum L.) cultivar Baraka with Hd3a flowering gene under control of a strong promoter prolC. Potato plants were propagated in vitro on MS2 medium. Genetic transformation was carried out by co-cultivation method by using Agrobacterium tumefaciens LBA4404. Leaf disc and internodes were used as explants for transformation. Selection of transgenic plants was performed in MS medium containing 10-40 mg/L hygromycin. The results showed that the efficiency of transformation was relatively low. The efficiency of shoot regeneration of transgenic calli was very high. The low efficiency of transformation was caused by damage of explant during transformation process. The efficiency of transformation of internode was higher than leaf explants. Putative transgenic shoot regeneration per callus was 2.5. In MS0 media, four of twenty-four putative transgenic plants produced tuber whereas all non-transgenic plants did not. This result showed that Hd3a induced the formation of tuber of transgenic potato cv. Baraka.
Keyword: Bogor Agricultural University (IPB), Hd3a flowering gene, genetic transformation, Solanum tuberosum L. cultivar Baraka

Judul: Asymptotic Distribution of Estimator for the Intensity Function of a Doubly Periodic Poisson Process
Abstrak: In this manuscript, estimation of the intensity function of a doubly periodic Poisson process is discussed. It is assumed that only a single realization of the Poisson process is observed in a bounded window [0,n]. It is also assumed that the period of the intensity function is known. The rate of consistency and asymptotic normality of the estimator are formulated. Finally, a confidence interval for the local intensity function is also formulated.
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