Judul: Penapisan Bakteri Lipolitik asal Fruktosfer Kelapa Sawit
Abstrak: Lipase (EC 3.1.1.3, glycerol ester hidrolase) is defined as carboxylesterase that catalyzes the hydrolysis of long chain asilgliserol. Lipase produced by lipolytic bacteria that grow well in substrates containing oils such as oil palm fruit. The purpose of this study was to screen lipolytic bacteria from oil palm fructosphere and to characterize optimal pH and temperature of lipase enzyme from selected isolates. Seven isolates of lipolytic bacteria with different morphology were qualitatively screened from oil palm fructosphere using Rhodamin B media. Bacteria were isolated from the exocarp and mesocarp of ripe oil palm fruits. Lipolytic activity each isolates were measured and selected based on their activity. Three isolates possessed the best activity with result as follows EP2.22 (0.912 U/mg), F2 (0.792 U/mg), and F6 (0.650 U/mg). These isolates showed high activity at various range of pH and temperature. Isolate EP2.22 showed optimum pH at 7.5 and optimum temperature at either 40 oC or 70 oC. Isolate F2 showed optimum pH 8.5 and optimum temperature 70 oC. Isolate F6 showed optimum pH 8.5 and optimum temperature at 30 oC and 70 oC. Two peaks of high activity due to the use of crude enzyme extract. 16S rRNA gene sequencing indicated that isolates EP2.22, F2, and F6 closely related to Staphylococcus klosii Enterobacter sp., and Buttiauxella izardii.
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Judul: Analisis Komunitas Bakteri Fruktosfer Kelapa Sawit Menggunakan Teknik Terminal Restriction Fragment Length Polymorphism (T-RFLP)
Abstrak: The knowledge about bacterial community could provide basic information for the development of further research. One method that could be used to analize the diversity of microbial community is Terminal Restriction Fragment Length Polymorphism (T-RFLP). The objective of this research is to analyze the existing bacterial diversity on oil palm fructosphere. The 16S rRNA genes were amplified by PCR using forward primer (27F) and reverse primer (926R) which have been labeled. The PCR product were then digested with four different enzymes, namely BstUI, MspI, DdeI, and Sau96I. The elecroforegram showed that these enzymes produce different patterns due to the unique restriction sites of each enzymes. In general, BstUI and MspI enzymes produce more Terminal Restriction Fragment (TRF) than two other enzymes. From T-RFLP analysis we could identify some bacterial genus. Bacteria that were predicted to be present in oil palm fructosphere were gram-positive and gram-negative bacteria, Proteobacteria, Actinobacteria, soil bacteria, marine bacteria, and bacteria that could not be cultured.
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Judul: Implementasi Space Time Composite (STC) Data Model Menggunakan Model Data Relasional
Abstrak: The data model is a concept that describes the data stored in the database and the relationship between the data to the user in logic. Development of data models is very important in the design of the database as the basis of the stage of database design to be made. This study aims to apply the concept of Space Time Composite (STC) on the Model Data storage vector data types that can store enough information and describes the process of drafting objects and objects spatiotemporal evolution over time. From the results of the test queries on the data model that have been created, queries in a system are acquired that can help display the information preparation of objects from time to time and know the history of the object polygon and arc that has undergone changes in topology.
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