Judul: Pengaruh Pemberian Ekstrak Minyak Jintan Hitam (Nigella sativa) terhadap Gambaran Histopatologi Organ Testis Mencit (Mus musculus)
Abstrak: This study was aimed to get information about Habbatussauda or blackseed (N. sativa) suplementation effect on mice testicle. Thirty six mices of 4 weeks were divided into four group, each group consists of nine mices. Group I was negative control (received 0.1 ml aquadest), group II (received 0.1 ml Habbatussauda oil), group III (received 0.2 ml Habbatussauda oil), and group IV (received 0.3 ml combination of Habbatussauda oil and honey). This treatment were done for two month, the mice were euthanized and then necropsied followed by testis collection as organ sample. Sample was processed to prepare histopathology slides with Hematoxilin-Eosin stain. The parameters observed include to count on and differenciate of the spermatogenic cells, Sertoli cell, and Leydig cell using software Image J® for Microsoft® Windows®. Qualitative data’s were analyzed with software SPSS® 16.0 for Microsoft® Windows® ANOVA test and followed by Duncan test. The result showed that Habbatussauda caused increasing of spermatogenic cells, Sertoli cell, and Leydig cell on group II, III, and IV which were significant (p<0.05) with the control group.
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Judul: Pengaruh Pemberian Ekstrak Minyak Jintan Hitam (Nigella sativa) terhadap Gambaran Histologi Organ Lambung dan Usus Halus Mencit (Mus musculus)
Abstrak: Habatussauda or black cumin seed (Nigella sativa) is a popular herbal medicine worldwide. The aim of this research was to observe histology changes on stomach and small intestine of mice treated with Nigella sativa oil extracts. Seventy two 4-week-old mice, consisting of thirty six male and female, were divided each into four groups. Group 1 (control) were treated with 0.1 ml of aquadest, group 2 with 0.1 ml of N. sativa oil extract, group 3 with 0.2 ml of N. sativa oil extract, and group 4 with 0.3 ml of combined N. sativa oil etxract and honey. All treatment were done orally everyday for two months period. Afterwards, mice were euthanized by atlanto-occipitale dislocation for organs sampling. Stomach and small intestine were fixed in 10% Buffered Neutral Formaline (BNF), embedded in paraffin, then stained with haematoxylin and eosin (HE) and periodic-acid schiff (PAS). The results showed that N. sativa treatment significantly increased (P<0.05) the number of parietal and chief cells, and reduced the number of mucous cells and infiltrating inflammatory cells in stomach; while, increased height of villi, increased number of crypts, reduced number of inflammatory cells, and reduced number of goblet cells were observed in small intestine, as compared with those in control. Additionally, there were no significant differences (P>0.05) in all parameters between male and female mice. These data may suggest cell protection and inflammatory cells suppresion activities of N. sativa treatment in gastrointestinal tracts.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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