Judul: Pengaruh Penambahan Asam Amino dalam Medium Kultur Bebas Serum terhadap Perkembangan Preimplantasi Embrio Mencit In Vitro
Abstrak: Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan asam amino esensial minimum (MEM) dengan clan tanpa asam amino non esensial glutamin ke dalam medium kultur MI6 bebas serum terhadap perkembangan embrio mencit in vitro. Pada penelitian ini digunakan medium MI6 modifikasi dengan penambahan 1.0 mg polivinyl alkohol ml" . Sehagai perlak~ian pada medium dasar dilakukan penambahan : (1) M16 + 0.3% BSA, (2) MI6 + 2% vlv MEM dan (3) MI6 + 2% vlv MEM + 0.1 mmolglutamin. Embrio yang dikultur dikoleksi pada 3 tahapan perkembangan yaitu: sigot (h-1), morula (h-3) dan morula kornpak (h-4). Parameter yang diamati adalah jumlah dan persentase embrio yang berkembang mencapai tahap 2 sel, 4 sel, 8 sel, morula kompak, blastosis, blastosis ekspan dan hatching.
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Judul: Pengaruh fosfat, glukosa dan kombinasinya dalam medium kultur in vitro terhadap perkembangan embrio mencit
Abstrak: Pada penelitian ini digunakan medium _Ml6 modifikasi yaitu medium Ml6 tanpa fosfat dan atau glukosa. Konsentrasi fosfat yang ditambahkan. dalam medium kultur masing-masing adalah 0,5 mmol/liter, 1,0 mmol/liter, 1,5 mmol/liter dan kontrol. Konsentrasi glukosa yang ditambahkan dalam medium kultur adalah 2,5 mmol/liter, 5,0 mmol/liter, 7,5 mmol/liter dan kontrol. Parameter yang diukur adalah jumlah dan persentase sigot mencit yang berkembang mencapai embrio tahap 2 sel, 4 sel, 8 sel, morula dan blastosis. Hasil penelitian menunjukkan bahwa konsentrasi fosfat 1, 0 mmol/liter dapat mendukung perkembangan embrio mencapai tahap blastosis. Pada tahap 2 sel konsentrasi fosfat 0,5 mmol/liter lebih baik mendukung perkembangan embrio, karena embrio yang berkembang bisa mencapai 64,6%, walaupun setelah dilakukan uji lanjut tidak berbeda nyata dengan konsentrasi fosfat 1,0 mmol/liter. Pada tahap 4 sel . kebutuhan fosfat cenderung meningkat dalam batas batas tertentu. Hal ini tampak pada konsentrasi fosfot 1, 0 mmol/liter jumlah embrio yang berkembang lebih tinggi dibandingkan dengan konsentrasi yang lain yaitu 5 I, 6%. Kemudian pada tahap 8 sel, morula dan blastosis jumlah embrio yang berkembang menurun, namun demikian konsentrasi fosfat I, 0 mmol/liter masih lebih baik dibandingkan dengan konsentrasi yang lain karena embrio yang berkembang mencapai 25%, 18,8% dan 9,4%. dst ...
Keyword: Kultur in vitro

Judul: Thermal Characteristics and Dynamics of Tropical Cyclone over Western North Pacific Ocean: A Case Study of Typhoon Songda (Chedeng) 2011
Abstrak: The frequent occasion of Tropical Cyclone (TC) over Western North Pacific (WNP) Ocean lies from 10˚N to 26˚N and 121˚E to 170˚E. Sources of TC development come from warm ocean temperature where latent heat is released during condensation processes and subsequently concentrated in the boundary layer. Relative humidity at 850-700 hPa is responsible to produce the most intense storm. Meanwhile, weak vertical wind shear between 850-200 hPa is one of the dynamical factors that lead to the development of TC. Pressure level of 850-200 hPa is chosen to minimize the thermodynamic effect in the lower troposphere. Typhoon Songda (Chedeng in the Philippines) or TSC lasted from May 18th to 30th, 2011 and strengthened into powerful Super Typhoon stage between May 24th and 27th. Long curving track and the exhibition of all sequences of TC life cycle, TSC is then considered as an ideal case of TC. The objective of the study with case of TSC is to investigate the role of thermal characteristics and dynamics on TC intensity. The research is carried out by using Advanced Research of Weather Research and Forecasting model (WRF ARW model) version 3.3. Two domains are employed in the model. The first domain situate from 02˚N to 40˚N and 118˚E to 149˚E and cover spatial resolution () of 33.1 km. The second domain situate from 09˚N to 27˚N and 119˚E to 132˚E with of 11 km. Timestep of the model () is 120 seconds. The model is supported by the following physical parameterization, i.e., Yonsei University Scheme (YSU) in the boundary layer, Kain-Fritsch scheme (KF) in the cumulus, and WRF Single-Moment 3-class scheme in the microphysics. Initial meteorological condition prior to the formation of the cyclone is generated using WRF ARW standard-release model. Sensitivity of the model output is obtained from a selected single-parameter change scenarios, namely, 10 % to 20 % decrease and increase of wind shear initial data at 850-200 hPa, and by 10 % to 20 % decrease of relative humidity initial data at 850-700 hPa. The result showed that the negative correlations between wind shear and intensity are found in wind shear scenarios. Wind shears of 10 ms-1 which lead to strengthening of the wind intensity in the early period of Typhoon development are found in wind shear scenarios. The effect of wind shear changes on wind intensity is statistically significant in the 20 % wind shear scenarios. During mature stage, weak wind intensity occurred when wind shear increase as given in the WRF standard-release and the 20 % decreasing scenario. Different situation is found in the 20 % increasing scenario where wind shear tend to weak and lead to strong wind intensity at this stage. Meanwhile, the reduction of moisture supply at 850-700 hPa resulting from 20 % decreasing relative humidity play a major role in the weakening of wind intensity and reduces mechanical energy of the cycle process as much as 300 J kg-1.
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