Judul: Perancangan ulang ruang terbuka hijau Kompleks Rehabilitasi Vokasional Bina Daksa Cibinong, Kabupaten Bogor
Abstrak: The National Vocational Rehabilitation Centre (NVRC) is the technical (practical) implementation unit in the field of vocational rehabilitation for disabled people, subordinate and responsible to the Ministry of Social Affairs. The main task and function of NVRC is to conduct advanced vocational rehabilitation services for people with disabilities from regional rehabilitation centres for the physically disabled, sheltered workshop and others. This research take places in NVRC Cibinong, Bogor with total area 35,419 m2 which consist of building area (12,695 m2) and greenery open space area (22,742 m2). The objectives of this research are (1) to analyze the existing conditions, (2) to describe the utilization concept of recreation-based on outdoor activity and (3) to make a recommendation design and suggestion as problem solving for accommodating disabled people in the specific greenery open space. This research consists of some steps, such as initial preparation, collecting data, analysis, and synthesis, forming concept, preparing landscape plan and the final is detail design. There are many functions of greenery open space, one of them is being used for outdoor activity. Greenery open space for outdoor activity needs to be serve in a good condition of society that had overwhelmed of physically, psychology, and life necessity pressure. In analysis and synthesis process, there is so many alternative of exploiting potency and solution of constraint in greenery open space of NVRC Cibinong, Bogor. As a result, the main concept of redesign is a greenery open space that is functional and aesthetic. Functional means having facilities for user with physical disabilities to do the gardening activity and relaxation activity. The gardening activities consist of seeding, planting, maintaining, harvesting, and postharvesting. Beside that, greenery open space of NVRC Cibinong, Bogor has function as a stabilizer of microclimate and habitat of wildlife animal (deer). In planning and design process, the greenery open spaces of NVRC Cibinong, Bogor divided by three areas. The areas are active recreation area (1,724 m2), passive recreation area (370 m2), and conservation area (4,356.58 m2). Each area will be put facilities and circulation patterns to accommodate user activity. The result of this study can be used as a reference and suggestion to other rehabilitation centre as an alternative utilization of greenery open spaces for people with disabilities.
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Judul: Design of Marzoeki Mahdi Hospital Green Open Space as disabled friendly park in Bogor City
Abstrak: Ruang Terbuka Hijau RS Marzoeki Mahdi dikelola oleh RS Marzoeki Mahdi sebagai rumah sakit kelas A dengan skala rujukan nasional. Ruang terbuka hijau ini memiliki peruntukan sebagai pembangunan gedung serbaguna untuk kegiatan rumah sakit. RS Marzoeki Mahdi sebagai pusat rujukan nasional memiliki potensi untuk dikunjungi oleh beragam pengguna. Oleh karena itu, Ruang Terbuka Hijau RS Marzoeki Mahdi seharusnya mempertimbangan kebutuhan disabilitas. Penyandang disabilitas juga terlihat jarang menggunakan ruang terbuka publik, khususnya taman. Penelitian ini bertujuan mengidentifikasi serta membuat rancangan desain ruang terbuka hijau RS Marzoeki Mahdi sebagai taman ramah disabilitas yang estetik dan fungsional. Penelitian ini juga mendukung program RS Marzoeki Mahdi dan Pemerintah Kota Bogor, serta menganalisis preferensi dan persepsi masyarakat penyandang disabilitas. Penelitian ini menggunakan metode Booth 1983, meliputi: 1) project acceptance; 2) research and analysis; 3) concept; 4) construction drawing. Output dari penelitian ini berupa site plan, gambar potongan-tampak, detail engineering design, dan ilustrasi desain., Marzoeki Mahdi Hospital Green Open Space is managed by Marzoeki Mahdi Hospital as a class A hospital with a national reference scale. This green open space has a designation as the construction of a multipurpose building for hospital activities. Marzoeki Mahdi Hospital as a national referral center has the potential to be visited by various users. Therefore, the Green Open Space of Marzoeki Mahdi Hospital should consider the needs of people with disabilities. People with disabilities are also seen rarely using public open spaces, especially parks. This study aims to identify and design a green open space design at Marzoeki Mahdi Hospital as an aesthetic and functional disability-friendly park. This research also supports the Marzoeki Mahdi Hospital program and the Bogor City Government, as well as analyzes the preferences and perceptions of people with disabilities. This study uses the 1983 Booth method, including: 1) project acceptance; 2) research and analysis; 3) concepts; 4) construction drawings. The output of this research is in the form of site plans, sectional drawings, detailed engineering designs, and design illustrations.
Keyword: disabled-friendly park, green open space, landscape, disabilities, hospital

Judul: Ekspresi Gen Penyandi Farnesoic Acid O-Methyl Transferase (Famet) Pada Organ Mandibular Kepiting Scylla Olivacea Fase Intermolt Dan Premolt
Abstrak: Farnesoic Acid O-Methyl Transferase (FAmeT) merupakan enzim untuk mengkonversi Asam Farnesoat (AF) menjadi Metil Farnesoat (MF) sebagai salah satu hormon yang berperan dalam proses metamorfosis dan reproduksi pada krustasea. Tujuan penelitian ini adalah untuk mengetahui tingkat ekspresi gen penyandi FAmeT pada fase sebelum molting yaitu intermolt dan premolt serta mengetahui urutan oligonukleotida parsial cDNA penyandi FAmeT kepiting Scylla olivacea. Analisis tingkat ekspresi gen dilakukan dengan melakukan ekstraksi RNA organ mandibular kepiting bakau pada masing-masing fase dan dilanjutkan dengan reverse transcription polymerase chain reaction (RT-PCR). Produk PCR diukur konsentrasinya dengan spektrofotometer dan pendaran RNA pada gel diukur ketebalannya dengan software UN-SCAN IT. Produk PCR yang dihasilkan kemudian dipurifikasi dan dilakukan pengurutan oligonukleotida. Hasil pengurutan oligonukleotida digunakan untuk mengetahui tingkat kesamaan urutan oligonukleotida cDNA penyandi FAmeT Scylla olivacea dengan urutan oligonukleotida cDNA penyandi FameT Scylla serrata, Scylla paramamosain dan Portunus. Hasil penelitian menunjukan bahwa konsentrasi produk PCR cDNA penyandi FAmeT pada organ mandibular kepiting fase premolt lebih tinggi dibanding pada fase intermolt walaupun perbedaannya tidak begitu signifikan. Hasil kuantifikasi konsentrasi produk PCR cDNA penyandi FAmeT menggunakan spektrofotometer menunjukkan rata-rata konsentrasi produk PCR pada fase intermolt adalah 765.4 ng/μl ±9.2, sementara pada fase premolt adalah 790.3 ng/μl ±15.1. Nilai rata-rata hasil kuantifikasi konsentrasi ekspresi berdasarkan ketebalan pendaran pita cDNA penyandi FAmeT pada gel adalah 412.1 ng/μl ±143.3 pada fase intermolt dan fase premolt adalah 442.5 ng/μl ±137.2. Hasil pengurutan oligonukleotida cDNA penyandi FAmeT Scylla olivacea menunjukkan urutan oligonukleotida yang hampir identik dengan 2 jenis Scylla lainnya yaitu Scylla serrata dan Scylla paramamosain yang sebelumnya telah dilaporkan dan didaftarkan di GenBank.
Keyword: Scylla olivacea, enzim, FAmeT, ekspresi gen