Judul: ting Style, Father-Adolescence Interaction, Attachment, and Father’s Satisfaction.
Abstrak: Penelitian ini bertujuan untuk mengetahui hubungan antara gaya pengasuhan, interaksi ayah-remaja, kelekatan, dan kepuasan ayah. Desain yang digunakan dalam penelitian ini adalah cross sectional study, melibatkan 60 ayah, terdiri atas 30 ayah dengan remaja laki-laki dan 30 ayah dengan remaja perempuan yang berusia 12-14 tahun. Tempat penelitian ini adalah di Kelurahan Panaragan, Kecamatan Bogor Tengah. Data dianalisis secara deskriptif, uji beda t-test, dan korelasi Pearson. Hasil menunjukkan bahwa hampir seluruh remaja mempersepsikan diasuh dengan menggunakan gaya pengasuhan demokratis. Pola komunikasi yang digunakan dalam keluarga sebagian besar adalah conversation-orientation dengan tipe komunikasi consensual. Rata-rata waktu dalam sehari yang diberikan oleh ayah untuk berinteraksi dengan remajanya adalah 0,47 jam untuk remaja laki-laki dan 0,64 jam untuk remaja perempuan. Hubungan yang postif signifikan terdapat antara gaya pengasuhan demokratis dengan pola komunikasi conversation-orientation. Pola komunikasi conversation-orientation yang diterapkan oleh ayah berhubungan positif dengan dimensi kepercayaan pada kelekatan. Pola komunikasi conformity-orientation yang dilakukan oleh ayah berhubungan negatif signifikan dengan tingkat kepuasan
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Judul: Gaya Pengasuhan, Pola Komunikasi, Kelekatan, dan Hubungannya dengan Kepuasan Remaja
Abstrak: This research aimed to analyze parenting style, communication pattern (communication type and time allocation), attachment, and correlation with adolescence’s satisfaction. The location of this research is in SMP Negeri 1 Dramaga and adolescence’s house arround Dramaga Subdistrict, Bogor District. This research used cross sectional design, involved 30 male adolescences, 30 female adolescences and 60 mothers as participant. The samples choosen by stratified random sampling. Data was collected through interview using structured questionnaire. Data was analyzed by descriptive, independent sample t-test, and Pearson corellation analysis. The result of this research shows that mostly adolescence have democratic parenting style perception. Almost two-thirds of male adolescence perceive communication type structural traditionalism, more than twothirds female adolescence and mother perceive family communication types expresiveness. More than half adolescence and mother have 5-15 minutes each day for communication each other. The categorize of attachment is high security for all adolescence and more than three-fourth adolescence’s satisfaction is average. There was positive significant correlation between parenting style with communication type, communication type with attachment, and attachment with adolescence’s satisfaction
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Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.