Judul: Tingkat kelayakan dan pengaruh lingkungan terhadap kualitas air sumur gali: studi kasus di Kecamatan Tegalsari dan Wonocolo Kotamadya Surabaya
Abstrak: Tujuan penelitian ini ialah untuk mengevaluasi tingkat kelayakan air sumur di Kotamadya Surabaya bagi kepentingan domestik dan untuk mengkaji faktor-faktor lingkungan penyebab penurunan tingkat kelayakan kualitas air minum..dst
Keyword: daur hidrologi, iklim, hidrologi, air limpasan, air sumur

Judul: Kualitas Air Sumur-Gali di Kotamadya Yogyakarta Bagian Selatan dan Kemungkinan Pengaruh Lingkungan Pemukiman
Abstrak: Tujuan penelitian ini ialah untuk mengetahui kualitas air sumur-gali di wilayah di Kotamadya Yogyakarta bagian selatan dengan mempertimbangkan kriteria baku untuk air-mimum, untuk mengetahui apakah faktor-faktor lingkungan pemukiman, lokasi sumur-gali dan wilayah pemukiman mempengaruhi kualitas air sumur-gali. Penelitian ini dilakukan di tiga wilayah RK yaitu Gengkiwo, Kaparakan dan Tegalgendu yang masing-masing terletak di kecamatan Mantrijeron, Margangsan dan Kota Gede dan masing-masing terletak di sepanjang daerah aliran sungai (FAS) Winogo, Code dan Gajahwong. Tiga puluh sumur-gali diambil sebagai contoh, dikelompokkan masing-masing 10 untuk tiap RK, dan ditetapkan dengan cara acak berlapis (stratified random sampling) untuk memperoleh masing-masing 5 sumur dekat sungai (jarak kurang dari 150 m dari tiga maksimum aliran sungai) dan 5 sumur jauh dari sungai (jarak lebih dari 300 m dari tepi maksimum aliran sungai)... dst
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Judul: Identification of Pheromone Binding Protein Gene of Yellow Rice Stem Borer Scirpophaga Incertulas (Walker) (Lepidoptera: Crambidae)
Abstrak: The yellow rice stem borers (YRSB) moth, Scirpophaga incertulas (Walker) (Lepidoptera: Crambidae), is known as the most major rice stem borer in tropical Asia. Pesticides are not effective to control the population of these insects due to almost entire larvae phase and pupae are in the rice stem. Hence, other control technique is needed such as based on the mating behaviour. Pheromone binding protein (PBP) in male S. incertulas antennae plays a role in the recognition of sex pheromone produced by the female, therefore influenced in their mating behavior. The aim of this study was to identify PBP gene of S. incertulas. For the purpose to collect imagoes as DNA source, S. incertulas were reared. Touchdown PCR and touchdown-nested PCR were the main techniques conducted to identify genomic of PBP gene from S. incertulas and revealed 700 and 600 bp amplicons, respectively. Those amplicons strongly expected as PBP gene. Sequence analysis of S. incertulas from touchdown-nested amplicon identified 575 bp which was consisted of 169 bp of exon 3 and 406 bp of intron 2. This study revealed putative amino acid sequences of exon 3 from S. incertulas has one conserved cysteine while other Lepidopterans PBP have three conserved cysteine. In phylogenetic analysis, the putative amino acid sequences obtained, showed a phylogenetic signal i.e. by clustering with PBPs from other Crambidae moths. The result of this study is important as a basic data for PBP expression analysis in female or male S. incertulas as the initial step to develope new insect biocontrol.
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