Judul: Mitochondrial DNA Gene Organization and Phylogenetic Relationship of Naja siamensis
Abstrak: Naja siamensis is one of Asiatic cobra in the Family of Elapidae, known as a cryptic snake. It has many morphological variations that lead to misidentification. Misidentification can cause the wrong concept in developing antivenom types due to high snakebite cases occur in Thailand. Thus, an accurate species identification using a molecular approach is crucially required. Mitochondrial DNA has been used widely in snake molecular studies. These studies were aimed to determine the arrangement of the N. siamensis partial mitochondrial genes using reptile-oriented primers and to reveal the phylogenetic relationship among other species of Asian cobra. DNA extraction was carried out based on the standard DNA salting-out protocol and was conducted by bioinformatics analysis using BLASTn and MEGA 6 software. Gene annotation was performed against N. kaouthia complete genome. A total of 3121 bp of N. siamensis mtDNA genes were sequenced consisting of partial of six regions: 16S rRNA, ND1, tRNA-Ile, CRII region, tRNA-Leu, tRNA-Gln, and COI genes. The mtDNA gene organization of N. siamensis was predicted to has a similar pattern with the common Elapidae mtDNA gene organization. This study showed that there is a monophyletic form in Naja genus and N. kaouthia is the closest species with N. siamensis based on partial 16S rRNA and ND1 gene.
Keyword: 16S rRNA, Elapidae, ND1, phylogenetic relationship

Judul: The Optimization specific primer of Varanus salvator (Water Monitor Lizard) Species Based on COI Gene (Cytochrome Oxidase Subunit I)
Abstrak: Reptil dan produk olahannya telah banyak dimanfaatkan di dunia. Permintaan untuk produk reptil termasuk kulit, daging, dan bahan untuk obat tradisional cukup tinggi. Pengembangan teknik deteksi dan identifikasi genus pada hewan dapat menjamin keaslian produk dan melindungi konsumen dari pemalsuan. Gen COI (cytochrome oxidase subunit 1) berguna dalam menyimpulkan hubungan intraspesifik karena penanda ini menghasilkan jarak genetik intraspesifik tertinggi jika dibandingkan dengan mitochondrial gene lain seperti ND5 (NADH-ubiquinone oxidoreductase chain 5 protein) dan Cyt b (Cytochrome b). Penelitian ini bertujuan melakukan optimasi primer spesifik spe-sies Varanus salvator menggunakan marka spesifik berbasis gen sitokrom oksidase sub unit 1 (COI 1). Sumber DNA yang digunakan dalam penelitian ini berupa sampel daging dan abon daging biawak air. DNA pada daging biawak air teramplifikasi pada 688 pb., Reptiles and their processed products have been widely used in the world. Demand for reptile products including leather, meat and ingredients for traditional medicine is quite high. The development of detection and genus identification techniques in animals can ensure the authenticity of products and protect consumers from falsification information. The COI gene (cytochrome oxidase sub unit 1) is useful for inferring intraspecific relationships because this marker pro-duces the highest intraspecific genetic range when compared to other mitochon-drial genes such as ND5 (NADH-ubiquinone oxidoreductase chain 5 protein) and Cyt b (Cytochrome b). This study aims to optimize the primers of Varanus salvator species in meat using specific markers based on the cytochrome oxidase I (COI) gene. Sources of DNA used in this study were Water Monitor Lizard’s meat and abon. DNA in Water Monitor Lizard’s meat amplified at 688 bp.
Keyword: water monitor lizard, cytochrome oxidase subunit I (COI), primer

Judul: Uji Performansi Pemerangkapan Radiasi Surya Dengan Beberapa Jenis Plastik Pada Bangunan Tembus Cahaya
Abstrak: Penanganan pasca panen mempunyal peranan penting untuk mencegah terjadinya kerusakan produk pertanianlperkebunan. Salah satu usaha yang dilakukan adalah pengeringan. Petani di tingkat pedesaan lebih banyak memilih pengeringan dengan penJemuran, mengingat cara ini lebih mudah dilakukan dan biaya lebih murah. Pemanfaatan efek rumah kaca sekarang ini sudah mulai dikembangkan. Sebagai pengganti kaca, altematif bahan yang dapat digunakan sebagai penutup bangunan adalah plastik. Penggunaan plastik ini diharapkan dapat menekan biaya pembuatan dan mudah didapati dipasaran.
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