Judul: Q-fever: suatu tinjauan perkembangan teknik diagnostik dan permasalahannya
Abstrak: Q-fever is a zoonoses that caused by Coxiella burnetti. Q-fever case in Indonesia was rarely reported. This was happen because of lack of knowledge in Qfever diagnose. The study of the disease diagnose technique become important and useful for Q-fever controlling in the field. This research was objected to study the Qfever diagnose technique and its problem. The first serologic diagnose technique used was the serological method and aimed to identify antibody (IgG and IgM) titer to C. burnetii. Serological method that firstly used was the Capillary tube in 1969 done by Fiset et al. In 1980s complement fixation test (CFT), immunofluorescence assay ([FA), and enzyme-linked immunosorbent assay (ELISA) were developed. CFT was also developed for diagnose the acute or chronic Q-fever specifically. However, CFT was less sensitive and costly than ELSA and IFA (Maurin & Raoult 1999). In 1999, IFA was used by Maurin and Raoult and became reference diagnose technique for Qfever. Although the test has high specificity and sensitivity but the result of the test was subjective (Slaba et al. 2005). ELISA diagnose technique has high specificity and sensitivity and could done the screening test in large number and very simple to use because it can be done automatically (field et al. 2000). However, the test was difficult to interpreted than the IFA (Maurin & Raoult 1999). The other method is PCR. PCR is the very sensitive method in laboratory diagnose for Q-fever. The PCR that commonly use are classic PCR, First-round PCR, Nested PCR and Real Time PCR. DNA sequencing has also developed, however this method is expensive and limited in small scale (Zhang et al. 1997).
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Judul: Molecular Detection of Coxiella burnetii Causes of Zoonotic Q Fever in Cattle at the Bandung Regency Slaughterhouse
Abstrak: Query fever (Q fever) merupakan penyakit zoonosis yang disebabkan oleh bakteri Gram-negatif bersifat obligat intraseluler yaitu Coxiella burnetii. Hewan ternak menjadi sumber utama penularan pada hewan lainnya maupun manusia. Sapi termasuk salah satu hewan sangat rentan terhadap infeksi akan tetapi umumnya tidak menunjukkan gejala klinis. C. burnetii pada sapi dapat menyebabkan abortus dan beberapa gangguan reproduksi lainnya. Distribusi C. burnetii sudah menyebar luas hampir ke seluruh dunia termasuk di Indonesia, dan penularan utamanya melalui aerosol. Studi dan laporan mengenai kasus Q fever pada hewan di Indonesia masih sangat terbatas hingga saat ini karena diagnosa klinis yang sulit dilakukan untuk deteksi dini. Kabupaten Bandung adalah salah satu wilayah di Jawa barat yang rentan terhadap penyebaran Q fever karena jumlah ternak yang tinggi khususnya sapi. Penelitian ini bertujuan untuk mendeteksi keberadaan C. burnetii sebagai penyebab Q fever pada berbagai organ sapi di Kabupaten Bandung. Sampel organ diambil dari 240 ekor sapi yang dipotong di empat Rumah Potong Hewan (RPH) di Kabupaten Bandung. Metode yang digunakan untuk mendeteksi keberadaan C. burnetii dalam sampel adalah nested Polymerase Chain Reaction (PCR) menggunakan primer outer membrane protein (OMP). Sampel positif hasil nested-PCR dilakukan pembuatan preparat histopatologi dengan pewarnaan hematoksilin-eosin (HE) untuk analisis lesi patologi. Lima ekor (2,08%) sapi Brahman cross terdeteksi positif C. burnetii yang berada dalam tiga organ jantung (1,25%) dan dua organ paru (0,83%). Gambaran histopatologi secara umum tidak menunjukkan adanya lesi signifikan dan spesifik terhadap infeksi C. burnetii melalui pewarnaan HE. Berdasarkan hasil tersebut, penelitian ini mengonfirmasi adanya infeksi subklinis C. burnetii pada populasi sapi di Kabupaten Bandung tanpa disertai lesi spesifik. Penelitian lanjutan mengenai Q fever diharapkan dapat dilakukan dengan beberapa metode lain seperti pewarnaan imunohistokimia atau sequencing., Coxiella burnetii, an obligate intracellular Gram-negative bacterium, is responsible for causing the zoonotic disease called Q fever. Livestock animals serve as the primary reservoir of infection for both animals and humans. Cattle are highly prone to infection; however, they typically remain asymptomatic. Coxiella burnetii in cattle can result in abortion and various other reproductive problems. C. burnetii has achieved global distribution, encompassing Indonesia, and its primary mode of transmission is by aerosols. There is a scarcity of studies and reporting on instances of Q fever in animals in Indonesia due to the challenging nature of clinical diagnosis for early detection. Bandung Regency, located in West Java, is susceptible to the transmission of Q fever due to its significant animal population, particularly cattle. The objective of this study is to identify the existence of C. burnetii as the etiological agent responsible for Q fever in cattle in Bandung Regency. Total 240 cattle slaughtered at four slaughterhouses (RPH) in Bandung Regency were used to collect organ samples. The technique employed for identifying the existence of C. burnetii in samples is nested polymerase chain reaction (PCR) with primers specific to the outer membrane protein (OMP). The PCR-positive samples were processed into histopathology slides and stained with hematoxylin-eosin (HE) for the examination of pathological abnormalities. Out of the total of five Brahman cross cattle, which accounts for 2,08% of the sample, three hearts (1,25%) and two lungs (0,83%) were found
Keyword: Q fever, Coxiella burnetii, nested-PCR, cattle

Judul: Pengaruh diameter dan jumlah paku terhadap kekuatan sambungan double shear pada balok kayu nangka (Artocarpus heterophyllus Lamk) dan rasamala (Altinga excelsa Noronha) dengan pelat baja
Abstrak: Seiring meningkatnya laju pembangunan dari tahun ke tahun. Akibatnya, banyak penggunaan kayu pendek khususnya untuk struktural sehingga diperlukan suatu sambungan pada batang-batang kayu untuk bisa mencapai bentang struktur yang dikehendaki. Awaludin (2002) mengatakan bahwa keberhasilan suatu kekuatan sambung salah satunya ditentukan oleh efektivitas suatu alat sambung. Paku sering dijumpai pada struktur dinding, lantai, dan rangka dalam bentuk dan ukuran yang bermacam-macam.
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