Judul: Karakterisasi Molekuler Defisiensi Enzim Glukosa-6-Fosfat Dehidrogenase di Kabupaten Sumba Tengah Nusa Tenggara Timur, Indonesia
Abstrak: Glucose-6-phosphate dehydrogenase deficiency is an X-linked recessive hereditary disease characterized by abnormally low levels of glucose-6-phosphate dehydrogenase (G6PD), the rate limiting enzyme in the pentose phosphate pathway that is important in red blood cell metabolism and the most common human enzyme defect. This research was aim to characterize and model the most common G6PD variants in Central Sumba’s people in order to help Indonesian goverment to design malaria treatment. Methodologies employed included nested PCR, gel electrophoresis purification, primer walking sequencing, and sequence alignment using BLAST. The most common G6PD variant were Vanua lava changing Leu to Pro at position 128 and Viangchan with Val changed to Met at position 291. From Pymol viewer software, substitution in Vanua Lava variant from 128Leu to 128Pro cause the instability of the helices while Viangchan substitution location was in tetrameric interface.
Keyword: Central Sumba district, G6PD deficiency, G6PD Variants, Molecular Characterization

Judul: Identifikasi Glycogen Storage Disease Tipe V pada Sapi Friesian-Holstein di Jawa - Bali
Abstrak: Glycogen storage disease (GSD) tipe V pada merupakan kelainan genetik autosomal resesif yang ditandai defisiensi enzim glikogen fosforilase. Glikogen fosforilase behngsi untuk memecah glikogen menjadi glukosa yang dibutuhkan dalam pembentukan ATP. Pada sapi, defisiensi glikogen fosforilase disebabkau oleh substitusi basa C menjadi T pada kodon ke-489 gen PYGM (muscle glycogen pliosphoiylase). Snbtitusi ini mengubah penyandian asam amino dari Arginin (cgg) menjadi Triptofan (tgg). Sapi karier GSD tipe V dapat menyebarkan ale1 niutan ke dalam suatn populasi melalni inseminasi bnatan (IB). Sapi FH merupakan sapi perah yang paling banyak ditemakkan di Indonesia dan dikembangbiakkan melalui IB. Perlu dilakukan identifikasi GSD tipe V dengan teknik polynierase chain reactioii-restrictiori pagmerzt leiigth poly~norpliisnz (PCR-RFLP) untuk mencegah penggunaan individu karier GSD tipe V sebagai bibit unggul sapi FH. Sampel darah sebagai sumber DNA diambil dari 7 pusat penlbibitan pemerintab dan 10 petemakan rakyat di Jawa-Bali dengan total 826 sampel. Semua sampel berhasil diamplifikasi dan individu lieterozigot tidak ditemukan. Hasil ini dapat menjaniin bahwit spenna atau induk betina yang didatangkan dari Jawa-Bali bebas dari kelainan genetik GSD tipe V.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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