Judul: Identification of Fish Species in Surimi Processed Seafood Products by Long Read Sequencing Method.
Abstrak: Produk olahan surimi tidak mencantumkan spesies ikan yang digunakan untuk bahan baku surimi pada label komposisi produk. Bahan baku surimi dikhawatirkan terbuat dari beberapa campuran spesies ikan yang merugikan dan membahayakan konsumen. Penelitian bertujuan mengidentifikasi spesies ikan sebagai bahan baku pada produk olahan komersial berbahan baku surimi menggunakan perbandingan teknologi long read sequencing dan Sanger sequencing. Penelitian dilakukan dengan ekstraksi DNA, amplifikasi DNA dengan PCR, pooling sampel, sekuensing dilakukan dengan dua metode yaitu Sanger sequencing dan Long read sequencing dengan teknologi Nanopore (ONT), serta analisis data bioinformatika. Sekuensing dengan metode Sanger terdeteksi 6 spesies dan sekuensing dengan Nanopore terdeteksi 20 spesies. Nanopore sequencing (ONT) mampu mendeteksi campuran spesies pada produk olahan surimi dengan sekali running. Metode Long read sequencing dengan teknologi terbaru Nanopore (ONT) lebih hemat waktu dan biaya dalam deteksi produk yang mengandung campuran spesies.
Keyword: DNA, PCR, Surimi, Nanopore sequencing, Sanger sequencing

Judul: Autentikasi dan Ketertelusuran Puffer Fish (Lagocephalus lunaris) pada Produk Olahan Ikan Berbasis Surimi
Abstrak: Produk olahan ikan berbasis surimi berpotensi tinggi dalam kesalahan pelabelan bahan baku, dan berpotensi menggunakan bahan tidak sesuai dan toksisitas tinggi. Penelitian bertujuan melakukan autentikasi untuk menentukan bahan baku digunakan pada produk olahan surimi menggunakan DNA barcode. Amplifikasi DNA dilakukan dengan primer target fish universal F1R1/F2R2, mini-barcode, serta primer spesies spesifik Lagocephalus lunaris. Amplifikasi optimum primer F1R1/F2R2 berada pada suhu 54 °C, sedangkan primer mini barcode pada suhu 62 °C dengan 35 siklus. Produk PCR di sekuensing, dan diketahui urutan basa nukleotida DNA sampel. Analisis menunjukkan sampel S1 adalah Coryphaena hippurus atau ikan mahi-mahi dengan homologi 96.32%, S2 dan S3 Nemipterus bathybius atau ikan kurisi dengan homologi 93.54% dan 94.77% dan CS adalah spesies Evynnis cardinalis atau Ikan kuro dengan homologi 97.80%. Amplifikasi sampel dengan primer spesies spesifik ikan buntal Lagocephalus lunaris dengan suhu 54 °C, 57 °C, dan 60 °C menunjukkan tidak terdapatnya pita DNA pada enam sampel yang dianalisis.
Keyword: DNA barcode, primer, produk surimi

Judul: Thermal Characteristics and Dynamics of Tropical Cyclone over Western North Pacific Ocean: A Case Study of Typhoon Songda (Chedeng) 2011
Abstrak: The frequent occasion of Tropical Cyclone (TC) over Western North Pacific (WNP) Ocean lies from 10˚N to 26˚N and 121˚E to 170˚E. Sources of TC development come from warm ocean temperature where latent heat is released during condensation processes and subsequently concentrated in the boundary layer. Relative humidity at 850-700 hPa is responsible to produce the most intense storm. Meanwhile, weak vertical wind shear between 850-200 hPa is one of the dynamical factors that lead to the development of TC. Pressure level of 850-200 hPa is chosen to minimize the thermodynamic effect in the lower troposphere. Typhoon Songda (Chedeng in the Philippines) or TSC lasted from May 18th to 30th, 2011 and strengthened into powerful Super Typhoon stage between May 24th and 27th. Long curving track and the exhibition of all sequences of TC life cycle, TSC is then considered as an ideal case of TC. The objective of the study with case of TSC is to investigate the role of thermal characteristics and dynamics on TC intensity. The research is carried out by using Advanced Research of Weather Research and Forecasting model (WRF ARW model) version 3.3. Two domains are employed in the model. The first domain situate from 02˚N to 40˚N and 118˚E to 149˚E and cover spatial resolution () of 33.1 km. The second domain situate from 09˚N to 27˚N and 119˚E to 132˚E with of 11 km. Timestep of the model () is 120 seconds. The model is supported by the following physical parameterization, i.e., Yonsei University Scheme (YSU) in the boundary layer, Kain-Fritsch scheme (KF) in the cumulus, and WRF Single-Moment 3-class scheme in the microphysics. Initial meteorological condition prior to the formation of the cyclone is generated using WRF ARW standard-release model. Sensitivity of the model output is obtained from a selected single-parameter change scenarios, namely, 10 % to 20 % decrease and increase of wind shear initial data at 850-200 hPa, and by 10 % to 20 % decrease of relative humidity initial data at 850-700 hPa. The result showed that the negative correlations between wind shear and intensity are found in wind shear scenarios. Wind shears of 10 ms-1 which lead to strengthening of the wind intensity in the early period of Typhoon development are found in wind shear scenarios. The effect of wind shear changes on wind intensity is statistically significant in the 20 % wind shear scenarios. During mature stage, weak wind intensity occurred when wind shear increase as given in the WRF standard-release and the 20 % decreasing scenario. Different situation is found in the 20 % increasing scenario where wind shear tend to weak and lead to strong wind intensity at this stage. Meanwhile, the reduction of moisture supply at 850-700 hPa resulting from 20 % decreasing relative humidity play a major role in the weakening of wind intensity and reduces mechanical energy of the cycle process as much as 300 J kg-1.
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