Judul: Strategi Pengelolaan Kawasan Hutan Berbasis Penguatan Kelembagaan Sosial Ekonomi Desa di Kabupaten Kampar, Provinsi Riau
Abstrak: Hutan di kabupaten Kampar, provinsi Riau telah mengalami kerusakan dan penurunan produktivitas. Kondisi ini terjadi didorong oleh pertambahan jumlah penduduk yang menyebabkan meningkatnya penawaran tenaga kerja sehingga menurut upaya percepatan pertumbuhan ekonomi di berbagai sektor. Kontradiksi yang terjadi antara kelangkaan sumberdaya hutan dengan kebutuhan untuk memanfaatkan sumberdaya tersebut akhirnya memperbesar resiko kerusakan pada kemampuan dan fungsi sumberdaya alam dan lingkungan. Potensi kerusakan hutan juga muncul dari perusahaan kayu yang beroperasi di kawasan ini sebagai pemilik HPH, dengan produksi kayu berkisar antara 10.000 sampai 39.000 meter kubik per tahun.
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Judul: Pengelolaan Kolaboratif Ekosistem Hutan Gambut Semenanjung Kampar, Provinsi RiauSupriatin
Abstrak: Various utilization to the natural resources at Kampar Peninsula has indicated resources degradation that could threaten its’ status. The aims of this research were to indentify natural resources’ characteristic, usage, and related issues; to analyze stakeholders’ of Kampar Peninsula management, its’ social capital, and related issues; to analyze policy and institutional arrangement; to analyze determinant variables of Kampar Peninsula management; and to formulate institution of Kampar Peninsula collaborative management. The study was conducted from February to May 2010 at Kampar Peninsula, using qualitative method. The data was collected through a collection of documents, field observation, in-depth interviews, focus groups discussion, and participate in meetings were related to the research topic. Data were analyzed qualitatively using stakeholders analysis, social capital analysis, and content analysis (policy analysis). The result showed that resources potency of Kampar Peninsula have degraded gradually by uncontrolled water management. More than 70 stakeholders were identified while regulation and policy that directly regulate peatland management least possible. The objective of Kampar Peninsula collaborative management is to realize sustainability Kampar Peninsula peat swamp forest ecosystem management on the landscape scale. Kampar Peninsula collaborative management can be realized through zoning that includes 5 main zones. Collaborative institution act as a "co-manager" for KPHP Tasik Besar Serkap and play the role as a "center of excellence". Meanwhile strengthening and institutionalization of social capital, mastering the technology of water management, strengthening the financial ability of the main actors, and the readiness of human resources are a precondition for realizing collaborative management.
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Judul: Influenza Specific Immunoglobulin Y Purification from Egg Yolk with Sodium Chloride Precipitation Method
Abstrak: Imunoglobulin Yolk (IgY) yang terdapat dalam kuning telur telah banyak dimanfaatkan untuk imunoterapi dan imunodiagnostik. Salah satu kendala dalam pemurnian IgY dari kuning telur adalah pemisahan Ig dengan lemak, sehingga teknik pemurnian Ig selalu berkembang. Penelitian ini bertujuan untuk melakukan pemurnian IgY dari kuning telur dengan metode pengendapan menggunakan sodium chloride (NaCl) serta melakukan karakterisasi IgY spesifik influenza. Sodium chloride dipilih karena bahan kimia ini relatif murah, ramah lingkungan, dan tidak merusak imunoaktivitas IgY. Kuning telur yang digunakan dalam penelitian ini berasal dari telur ayam layer yang divaksinasi dengan vaksin influenza Flubio® yang berisi virus Influenza A/H3N2, A/H1N1 dan B/Washington. Pemurnian IgY hanya dilakukan pada kuning telur yang mengandung antibodi spesifik influenza. Deteksi keberadaan antibodi spesifik influenza ditentukan dengan uji agar gel presipitasi (AGP) dan metode enzyme linked immunosorbent assay (ELISA). Konsentrasi IgY hasil pemurnian dihitung dengan spektrofotometer dan karakterisasi protein dilakukan dengan metode Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Konsentrasi protein dari hasil pemurnian IgY memiliki nilai rata – rata 50,403 mg/mL. Hasil SDS-PAGE menunjukkan enam pita protein, yaitu struktur IgY utuh (174,63 kDa), Apoprotein-LDL (129,77 kDa), rantai berat IgY (66,54 kDa), Fab ( 47,65 kDa), rantai ringan IgY (21,85 kDa), dan β-livetin (36,74 kDa). IgY hasil pemurnian masih dapat berikatan dengan antigen influenza berdasarkan uji ELISA. Hasil penelitian ini menunjukkan bahwa pemurnian IgY menggunakan metode pengendapan NaCl dapat memurnikan IgY dengan struktur utuh, meskipun masih ada dua protein tidak spesifik hasil pemurnian. IgY yang dapat berikatan dengan antigen influenza mengindikasikan prospek pemanfaatannya untuk pencegahan influenza dan imunodiagnostik., Yolk immunoglobulin (IgY) contained in egg yolk has been widely used for immunotherapy and immunodiagnostics. One of the obstacles in IgY purification from egg yolk is separating Ig from fat, so that IgY purification techniques are always developing. This study aimed to purify IgY from egg yolk using sodium chloride (NaCl) precipitation method and to characterize influenza-specific IgY. Sodium chloride was chosen because this chemical is relatively unexpensive, environmentally friendly, and does not impair IgY immunoactivity. The egg yolks used in this study were collected from layer eggs vaccinated with the Flubio® influenza vaccine, which contains the Influenza A/H3N2, A/H1N1 and B/Washington viruses. IgY purification was only carried out on egg yolks containing influenza specific antibodies. The presence of influenza-specific antibodies was determined by the agar gel precipitation (AGP) test and the enzyme linked immunosorbent assay (ELISA) method. The purified IgY concentration was determined using spectrophotometer, and protein characterization was performed using the Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) method. Protein concentration from purification has a mean value of 50,403 mg/mL . SDS-PAGE result showed six protein bands, namely intact IgY structure (174,63 kDa), Apoprotein-LDL (129,77 kDa), IgY heavy chain (66,54 kDa), Fab (47,65 kDa), IgY light chain (21,85 kDa), and β-livetin (36,74 kDa). The purified IgY still able to bind with influenza antigen based on the ELISA test. The results of this study indicate that purification of IgY using the NaCl precipitation method can purify IgY with intact structure, although there are still two unspecific proteins in the purification results. The resulting IgY can bind to influenza antigens indicating the
Keyword: Immunoglobulin Y, influenza, egg yolk, purification, sodium chloride