Judul: Selenoprotein W1 (SEPW1) Gene Polymorphism and Association with Mineral Content in Lamb
Abstrak: Selenoprotein W1 (SEPW1) adalah protein yang mengandung selenium yang memiliki peran penting dalam pertumbuhan dan perkembangan otot melalui perlindungan myoblast dari stres oksidatif. Penelitian ini bertujuan untuk mengidentifikasi keragaman gen SEPW1 dan hubungannya dengan kandungan mineral pada domba. Sampel yang digunakan sebanyak 40 ekor domba yang terdiri dari 10 domba ekor tipis (DET), 10 domba compass agrinak (DCA), 10 domba garut (DG), dan 10 domba jonggol (DJ). Kandungan mineral yang terpilih ditentukan dengan menggunakan metode IK LP-04.10-LT-1.0. Identifikasi polimorfisme gen SEPW1 dilakukan menggunakan Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Ukuran produk amplifikasi adalah 455 bp. Uji chi kuadrat menunjukkan populasi yang diamati berada dalam keseimbangan Hardy Weinberg. Keragaman gen SEPW1 ditemukan pada setiap rumpun domba. Hasil analisis menunjukkan SNP g.53617063 A>G gen SEPW1 berasosiasi secara signifikan (P<0.05) dengan kandungan mineral selenium. Gen SEPW1 berpotensi digunakan sebagai penanda seleksi terhadap kandungan mineral pada domba, Selenoprotein W1 (SEPW1) is a protein containing selenium that has an important role in muscle growth and development through protecting myoblasts from oxidative stress. This study was aim to identify polymorphism of the SEPW1 gene and its association with mineral content in sheep. The samples used were 40 sheep consisting 10 samples of javanese thin tailed sheep (JTT), 10 samples of compass agrinak sheep (CAS), 10 samples garut sheep (GS), and 10 samples of jonggol sheep (JS). Selected mineral contents were determined using the IK LP 04.10-LT-1.0 method. Identification of SEPW1 gene polymorphism were conducted using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The size of the amplification product were 455 bp. Chi-square analys showed that the population was in Hardy-Weinberg equilibrium. Polymorphism of SEPW1 gene were found in each breed of sheep. A SNP g.53617063 A>G of the SEPW1 gene were significantly associated (P<0.005) with selenium mineral content. The SEPW1 gene was potential to be used as a selection marker for mineral content in lamb.
Keyword: Mineral Content, PCR-RFLP, SEPW1 Gene, Sheep

Judul: Evaluasi Keragaman Gen Selenoprotein W (SEPW1) dan Asosiasinya terhadap Komposisi Asam Lemak pada Domba
Abstrak: Komposisi asam lemak dikontrol oleh beberapa gen, salah satu gen yang diduga memiliki peran dalam sifat ini adalah selenoprotein W (SEPW1). Penelitian ini bertujuan untuk mengidentifikasi keragaman gen SEPW1 dan asosiasinya dengan komposisi asam lemak pada domba. Total sampel yang digunakan sebanyak 55 ekor domba yang terdiri atas 20 domba ekor gemuk (DEG), 15 domba ekor tipis (DET), 10 domba komposit garut (DKG), dan 10 domba compass agrinak (DCA). Identifikasi keragaman SEPW1|BanI dianalisis menggunakan Polymerase Chain Reaction-Restriction Fragment Lenght Polymorphism (PCR-RFLP). Ukuran produk amplifikasi adalah 478 bp. Uji chikuadrat menunjukkan populasi yang diamati berada dalam keseimbangan Hardy- Weinberg. Keragaman gen SEPW1 ditemukan pada setiap rumpun domba. SNP (Single Nucleotide Polymorphism) g.53617063 G>A gen SEPW1 berasosiasi (P<0.05) dengan asam arakidat (C20:0) dan asam lemak total. SNP gen SEPW1 disimpulkan memiliki potensi untuk dikembangkan dalam pemuliaan ternak berbasis seleksi genomik untuk penentuan komposisi asam lemak.
Keyword: asam lemak, domba, gen SEPW1, PCR-RFLP

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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