Judul: Persepsi Masyarakat tentang Penyuluhan Pembangunan dalam Pembangunan Masyarakat Pedesaan
Abstrak: Tujuan penelitian ini adalah menelaah persepsi masyarakat tentang penyuluhan pembangunan. Secara terinci tujuan penelitian ini adalah (1) menelaah persepsi masyarakat tentang penyuluhan, pengaturan dan pelayanan dalam penyuluhan pembangunan masyarakat pedesaan; (2) menelaah keragaan perilaku dan efektivitas penyuluhan pada berbagai jenis penyuluhan pembangunan; dan (3) menelaah keterkaitan penyuluhan, pengaturan dan pelayanan dengan keragaan perilaku masyarakat pedesaan.
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Judul: Peranserta masyarakat dalam pembangunan desa di Propinsi DT II Sumatera Barat
Abstrak: Penelitian ini bertujuan untuk mempelajari peran serta, dan persepsi masyarakat dalmn pelaksanaan Program Inpres Pembangunan Desa serta faktor-faktor yang mempengaruhinya. Peranserta masyarakat dalam Program Inpres Pembangunan Desa dipelajari dengan mengambil 100 desa sebagai desa contoh. Sedangkan peubah-peubah bebas yang diduga berpengaruh adalah; (i) jumlah bantuan yang disediakan oleh pemerintah, (ii) luas lahan pertanian per KK tani, (iii) porsi keluarga yang mempunyai pekerjaan disektor non pertanian, (iv) porsi tenaga potensial, (v) porsi tenaga potensial yang berpendidikan SLTP keatas, (vi) tingkat kesehatan masyarakat, dan (vii) kepemimpinan formal di pedesaan. Pengaruh dari ketujuh peubah tersebut diatas terhadap peranserta masyarakat dalam pelaksanaan Program Impres Pembangunan Desa di analisis dengan menggunakan "Metoda pangkat 2 terkecil" atau Least Square Method. Antara peranserta masyarakat dan peubah-peubah bebas diduga terdapat hu- bungan model fungsi linear. ...
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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