Judul: Preferensi Pangan Anak Sekolah Dasar Di Kota Bogor
Abstrak: Food preference has an important role in defining children’s food pattern. Food preference that is formed earlier will persist to influence food preference in adult. In the other words, children’s food preference is a critical point in defining adult’s food preference. Thus, study about children’s food preference and many factors that were related is important to do. The general objective of this study was to identified factors that is correlate to food preference of school children in bogor city, and the specific objectivess were to: 1). Identified individual characteristic of subject, 2). Identified Family’s socio economics condition of subject, 3). Identified school children’s food preference, and 4). Analyze correlation between food preference and many factors. This study used cross-sectional design and the subject was 90 students from 3 elementary school in Bogor City. The correlation between factors and food preferences analized use inferensia statistics which are: Chi Square and Rank Spearman. Food preference of school children shows that school children tend to like certain kind of food in each food groups. There are several kinds of food in each foods group which is less like by school children. Fruits are liked by most school children. Most of school children like a kind of food because of the organoleptic characteristic of food, especially taste. Individual characteristics and family’s socio economic conditions explained little of the variation in school children’s food preference. The statistic test shows that preference of certain food has correlation with individual and family’s socio economic conditions. School children’s food preference doesn’t not show variety yet. Interventions are needed to direct school children’s food preference become more various.
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Judul: Pola Asuh Makan Ibu serta Preferensi dan Konsumsi Sayur dan Buah Anak Usia Sekolah di Bogor.
Abstrak: Tujuan umum penelitian adalah mempelajari kaitan pola asuh makan ibu dengan preferensi dan konsumsi sayur dan buah anak usia sekolah. Desain penelitian adalah cross-sectional dengan teknik penarikan sampel secara purposive sebanyak 108 sampel anak usia sekolah di SDN Cibanteng 1 Kabupaten Bogor dan SDN Papandayan Kota Bogor. Pengukuran preferensi menggunakan skala hedonik dan konsumsi menggunakan semi-quantitative food frequency questionnaire. Konsumsi sayur anak di kota (68.5±31.6 g/hari) lebih tinggi dibandingkan di kabupaten (45.4±18.7 g/hari). Konsumsi buah anak di kota (166.5±67.7 g/hari) lebih tinggi dibandingkan di kabupaten (106.9±43.0 g/hari). Terdapat hubungan signifikan negatif (p<0.050) antara peran ibu dalam pemberian makan dengan konsumsi buah anak. Terdapat hubungan signifikan positif antara tingkat kesukaan 8 jenis sayur (wortel, tauge, jagung muda, jamur, daun singkong, ketimun, kacang panjang, dan caisim) dan 3 jenis buah (manggis, pir, dan jambu air) dengan konsumsinya (p<0.050).
Keyword: konsumsi, pola asuh makan, preferensi pangan, sayur dan buah

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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