Judul: Dampak Ekonomi dan Ekologi Kelembagaan Pokja Situ Pengasinan Depok
Abstrak: The decreasing of quality and quantity of lake in Jadebotabek has prompted the local government to create work society (Pokja). Pokja is expected to be the solution of lake’s problems in Jadebotabek like Pokja Pengasinan Lake. The result of this research showed that Pokja in Pengasinan Lake has a good management system. Pokja Situ Pengasinan as a formal institution which has local society basis which is influenced by four stakeholders, consist of Pokja, Department of Highways and Water Resources, environmental agencies, and Department of Youth Sports and Tourism. The main roles of Pokja are saving and controlling Pengasinan Lake as water storage, it also has contribution to increase people interests in water tourism. Tourism activities in Situ Pengasinan push great economic impacts to the society and local community, not only direct and indirect impactt, but also induced impact. This is reflected in the value of the multiplier effect to the value of Keynesian income multiplier of 1.25, the ratio of type I income multiplier of 1.05, and a ratio of Type II income multiplier of 1.06.
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Judul: Upaya Pengelolaan Kualitas Perairan untuk Pengembangan Wisata Air Situ Sawangan-Bojongsari di Kota Depok
Abstrak: Development of situ into water tourism area is an effort to conserve situ from development activities damage, so that situ can generate ecological, economic, and aesthetic benefits. Situ Sawangan-Bojongsari is one of small lakes in Depok that is being developed into water tourism area. Accordingly, this research aimed to study the management, water quality, visitor perceptions, and the level of residents knowledge of small lake environmental material and water tourism development in Situ Sawangan-Bojongsari, and also make recommendation of water quality management strategies for water tourism development in Situ Sawangan-Bojongsari. The management of Situ Sawangan- Bojongsari must be improved by requiring cooperation and better coordinating between the stakeholders involved in: Center Government, Local Government, Working Group of Situ Sawangan-Bojongsari, Forum of Situ Working Groups in Depok, private sector, and NGOs. Water condition of this small lake tends to be good enough for the water tourism development, but some measurements of some parameters exceed or not conform with the water quality standard. The water quality of small lake can decrease more quickly because of anthropogenic activities. Perceptions of visitors to the condition of Situ Sawangan-Bojongsari and its tourism are good enough, but small lake management and water tourism facilities improvement should be performed. The levels of residents knowledge of the small lake environmental material and water tourism development are respectively quite good, but still need to be improved into good comprehension and attitudes. Based on hierarchy analysis, the community empowerment is an alternative to the highest priority, followed by socialization, and recommendation of area management.
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Judul: DNA Fragmentation on HCT-116 Colon Cancer Cell in the Presence of Short Chain Fatty Acid (SCFA) Extract
Abstrak: Type 3 resistant starch (RS3) is retrogaded starch that resists digestion in the small intestine of healthy people and fermented in the large intestine. The main fermentation products are the short chain fatty acids (SCFA) acetate, propionate, and butyrate. Butyrate is the major energy source for the colonocytes and have anti-carcinogenic effects mainly by affecting proliferation, differentiation, and apoptosis of colonocytes. The aim of this study was to analyze apoptotic DNA fragments of HCT-116 human colon cancer cell incubated with SCFA derived from fermentation of RS3 of sweet potato (Ipomoea batatas) by C. Butyricum BCC B2571 using DNA ladder assay. The DNA bands detected from attached cells treated with SCFA containing 1.00 and 1.25 mM butyrate were different from the control, appeared as longer smear. There were separate DNA bands in the floating cells in the treated medium (SCFA containing 0.625, 1.00, and 1.25 mM butyrate) at ±100 bp. These bands were not presence in the control. DNA fragmentation was not clear in the attached cells, but was observed as a separate bands in the floating cells in the medium which contained the dead cells. This research still needs optimazion of DNA extraction to get the best result of DNA fragmentation assay
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