Judul: Komponen Kimia Fraksi Nonpolar Ekstrak Metanol Daun Trengguli (Cassia fistula)
Abstrak: Many studies have been carried out on trengguli’s leaf due to its various secondary metabolites having bioactive properties. This study covered extraction, fractionation of chemical components, and characterization of compounds contained in the isolated fractions. The extraction was done by maceration of leaves sample in methanol. Phytochemical tests on the methanol extract indicated the presence of alkaloid, flavonoid, phenolic, tannin, and steroid as the secondary metabolites. Fractionation performed by vacuum liquid chromatography method produced 7 fractions. Analysis of fraction B on thin layer chromatography (TLC) indicated a single stain in various eluents, supported by the 2-dimensional TLC and spraying with cerium sulfate results. Identification by using gas chromatograph-mass spectrometer, combined with ultraviolet-visible and Fourier transformed infrared spectrophotometer suggested that fraction B contained nonpolar compounds, including is fatty ester.
Keyword: Cassia fistula, fatty ester, trengguli leaves

Judul: Prediksi Keberadaan Pelargonidin-3-(feruloil)diglukosida-5 (malonil)glukosida pada Fraksi Terlarut Etil Asetat dari Ekstrak Metanol Daun Trengguli (Cassia fistula)
Abstrak: Preliminary study has been conducted to identify bioactive compounds in trengguli (Cassia fistula) leaves. Traditionally, C. fistula leaves were used for treatment of malaria disease. Phytochemical investigations on leaves’ methanol extract of C. fistula from Bogor Botanical Garden showed the presence of alkaloid, flavonoid, phenolic, tannin, and steroid. Fractionation of the methanol extract was conducted through some chromatography methods and the results have been analyzed with nuclear magnetic resonance spectrophotometer and liquid chromatograph-mass spectrometer. The analysis showed that the n-hexaneethyl acetate 3:7 fraction was predicted to contain pelargonidin-3-(feruloyl) diglucoside-5-(malonyl)glucoside (m/z 1019) and various fatty acids.
Keyword: trengguli, malonyl)glucoside (m/z 1019) and various fatty pelargonidin, chromatography, Cassia fistula

Judul: Antibody Responses against Newcastle Disease and Evaluation of Infectious Bronchitis Maternal Derived Antibody on Vaccinated Layers.
Abstrak: Information of maternal derived antibody (MDA) and onset of immunity after vaccination is important to determine appropriate vaccination programme of chickens. The aim of this study was to evaluate duration of Infectious Bronchitis (IB) MDA and evaluate onset of immunity Newcastle Disease (ND) vaccination among layers. All layers were vaccinated using live ND and Infectious Bursal Disease (IBD) at one day old age, and received Avian Rhinotracheitis (AR) vaccine at 4 day old age. Serum samples were collected at various ages: 7, 10, 14, 20, 22, 24, 29 and 35 day old. The presence of ND antibodies was measured by the hemagglutination inhibition (HI) test, whilst the presence of IB antibodies was measured by enzyme-linked immunosorbent assay (ELISA). Our study showed that ND antibodies were at positive level until 24 day old age, and reached protective level ≥ 25 only at 7 day old age, whilst IB MDA were positive up to 17 day old age. This result indicating the needs of ND booster vaccination at least at 24 day old and IB vaccination at 17 day old age.
Keyword: antibody, Infectious Bronchitis, layers, Newcastle Disease, vaccination