Judul: Studi Kultur Kalus Tanaman Pegagan (Centella miatica L.) Untuk Menghasilkan Senyawa Asiatikosida
Abstrak: Pegagan ( Centella asiatica L.) herbs is one of medicinal herbs component which is often used in traditional treatments, especially as anti inflammation, diuretics, tonic, and hypertension, pheri very bleeding, leprosy, tuberculoses, quickening wound healing and acne healing. Namely h4adecassolR which is consist of Centella asiatica extract used in modem treatmen for preventing khelloid forming and quickening wound healing in dosage form as bblet, spread powder and cream. The drugs contain of Centella asiatica are asiaticoside, Asiatic acid and madecassac acid. The of Object of of this experiment was to study the possibility of production of asiaticosida by callus cultures of Centella asiatica L. Explant used was leaf and petiole of sterile obtained fiom in vitro cultivation which old about 6 month. The basal medium used was MS (Murashige and Skoog). Treatment growth regulation combinations was sitokinin BA and kinetin (0.5 mg/l, 1.0 mg/l) with auxin 2,4 D ( 0.0, 1.0, 3.0, 5.0 mg/l)Asiaticoside produced by callus were analyzed quantitatively using HPLC method. The result showed that highest mean of wet weight of callus by leaf explant (8 and 16 weeks after cutivating was 0.908 g and 3.248 g(BA 1.0 mg/l + 2.4 D 0.0 mg/l) and 1.024 g and 4.599 g (Kinetin 1.0 mg/l + 2,4D 0.0 mg/l)of callus by petiole explant.. The dry weight of highest mean of callus obtained at same treatment and like at wet weight highest mean of callus that is 0.221 g ( BA 1.0 g/lrn+2.4D0.0 d m ) and 0.193 g ( Kinetin 1.0 mg/l + 2,4D 0.0 mg/l). The result of analysis of HPLC indicate that callus existence of compound asiaticoside, the callus from leaf explant ( BA 1.0 mg/l + 2,4D 0.0 mg/l) asiaticoside compoud contents was 0.062 % per weight of sample callus. While at callus from petiole (Kinetin 1.0 mg/l + 2,4D 0.0 mg/l) asiaticoside compound contents was 0.079 % per weight of sample callus.
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Judul: Pengoptimuman Ekstraksi dan Pemurnian Asiatikosida dari Pegagan (Centella asiatica)
Abstrak: Extraction of asiaticoside from Centella asiatica has already reported but the effective extraction method should be continuously developed. This study was developed to find an effective extraction and purification method of asiaticoside from this species. Extraction parameter such as temperature, time, and solvent were varied and extraction was conducted using ultrasonic. Asiaticoside content from crude extract was determined using ultraviolet-visible spectrophotometer. Asiaticoside from the extract was further isolated by column chromatography with chloroform: methanol as using step gradient elution. The concentration and purity of asiaticoside fraction from preparative TLC was determined using high performance liquid chromatography analysis. The optimum extraction conditions was that using ethanol as solvent at 30 °C for 15 min. The result showed that the best eluent to separate asiaticoside fraction was chloroform: methanol in 6:4, 5:5, and 4:6 ratios. The asiaticoside contents of fraction 1 and 2 was 23:14 mg/g preparative TLC fraction and 193.75 mg/g preparative TLC fraction with purity less than 50%.
Keyword: extraction, Centella asiatica, asiaticoside

Judul: Cycle Hamilton pada Graf Lengkap, Graf Regular, dan Graf 2-Connected 4-Regular Berorder Kurang dari Sepuluh
Abstrak: Suatu graf disebut graf Hamilton apabila graf tersebut memuat cycle Hamilton, yaitu cycle yang melewati setiap vertex tepat satu kali. Menentukan keberadaan cycle Hamilton pada suatu graf dapat menggunakan syarat cukup yang berupa teorema-teorema yang telah diperkenalkan sejak tahun 1950-an. Beberapa syarat cukup yang dibahas dalam karya ilmiah ini adalah syarat cukup yang berhubungan dengan graf lengkap dan graf regular. Suatu graf lengkap berorder 𝑛������ ≥ 3 dan graf r-regular berorder 𝑛������ ≤ 2𝑟������ + 1 merupakan graf Hamilton, Karena kedua graf tersebut memuat cycle Hamilton. Selanjutnya, syarat cukup tersebut digunakan untuk menentukan keberadaan cycle Hamilton pada graf 2-connected 4- regular berorder kurang dari 10.
Keyword: graf Hamilton, graf regular, graf lengkap, graf 2-connected 4-regular