Judul: Induksi Kantong dengan Perlakuan Berbagai Konsentrasi Media Murashige & Skoog pada Beberapa Ukuran Eksplan Kantong Semar (Nepenthes gracilis Korth.) secara In Vitro
Abstrak: Nepenthes is a plant species which is classified as a unique ornamental plant that has the capability of adaptation to environments with poor nutrients by forming pitcher (Mansur 2007). Nepenthes gracilis is an example of Nepenthes species with high capability of adaptation. Nepenthes have benefits as medicinal and unique ornamental plant. This uniqueness lies in the pitcher which is a modification of the center part of the leaf. Pitcher as the most widely used part is a form of adaptation by Nepenthes, and it is not yet known for certain how the induction of the formation of a pitcher. Therefore, the research to know the response of pitcher formation in various concentrations of Murashige & Skoog medium on multiple size of N. gracilis Korth explants is needed. The aim of this research is to study the response of pitcher formation on various concentration of Murashige & Skoog medium on multiple size of N. gracilis Korth explants. This research was conducted on June until December 2012 at the Tissue Culture Laboratory, Center for Plant Conservation, Bogor Botanical Garden. The treatment in this study is the concentration of MS basic medium and explant size. The concentration of MS basic medium which is used are 1/2MS, 1/4MS, 1/8MS, 1/16MS and 1/32MS. The explant size of N. gracilis are < 2 cm, 2-4 cm and > 4 cm. Routine observations was done for 12 MSP and visual observations was done until December 2012. Parameter measurement which is observed every week are plant height, number of leaves and number of pitcher. Parameter measurement which is observed at the end of observation are the color of the leaf, the color of the pitcher, and the percentage of living and pitched planlet. The result shows that the concentration of MS basic medium and explant size influenced the number of leaves, explant height, and the number of pitcher. The concentration of 1/2MS and 1/4MS with the explant size < 2 cm, and 1/8MS with explant size 2-4 cm are treatments that gave the best influence on the formation of pitchers. Those treatments shows the average of the highest pitcher formation which is 3,00 to 3,50 pitchers.
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Judul: The Effect of BAP Hormones on Shoot Multiplication of Kantung Semar (Nephentes alata Blanco) Planted on Murashige & Skoog Media with In Vitro Techniques
Abstrak: Nepenthes atau Kantung Semar merupakan tumbuhan yang unik yang saat ini keberadaannya semakin terancam. Saat ini nepenthes termasuk ke dalam tanaman langka berdasarkan kategori IUCN (International Union for Conservation of Nature) dan WCMC (World Conservation Monitoring Centre). Di Indonesia tumbuhan ini dilindungi menurut PP No. 7 tahun 1999 dan termasuk dalam daftar CITES Appendix I (N. rajah dan N. khasiana) dan Appendix II (selain kedua jenis tersebut). Teknik budidaya tumbuhan dengan metode konvensional (menggunakan medium tanah atau pasir) sering dihadapkan kepada kendala teknis, baik dalam segi waktu maupun lingkungan. Oleh karena itu saat ini telah berkembang teknik budidaya kultur jaringan, dan untuk mengoptimalkan multiplikasi tunas N. alata dapat dirangsang dengan menggunakan hormon BAP (6-benzylaminopurine)., Nepenthes or pitcher plant is unique plant which existence is increasingly threatened. Currently nepenthes is belong to the category of rare plants on the basis of IUCN (International Union for Conservation of Nature) and WCMC (World Conservation Monitoring Centre). In Indonesia, this plant is protected by PP No.7 / 1999 and included in Appendix I (N. rajah and N. khasiana) and Appendix II (in addition to the two types of these) of CITES. Plant cultivation techniques with conventional methods (using soil or sand media) are often encounter technical challenges, both in terms of time and environment. Nowaday, tissue culture technique had been developed to overcome the challenges, and BAP hormones (6-benzylaminopurine) could be used in stimulating nepenthes shoot multiplication. The research was conducted in November 2010 to March 2011 in Plant Conservation Laboratory, Department of Forest Resources Conservation and Ecotourism, Faculty of Forestry, Bogor Agricultural University. The purpose of this study was to investigate the effect of BAP hormones (0, 0.1, 0.3, and 0.5 ml/l), the use of Murashige & Skoog media (0, 1/5, 1/3, and 1 MS), and the interaction of both hormone and media effect on the growth nepenthes invitro. Quantitative data collected included the number of shoots, books, and leaves, and the height of shoot, while the qualitative data included description of the condition of the plant (root, callus, and death). The result showed that the interaction between BAP hormone and Murashige & Skoog medium did not indicate a statistically significantly different effect on the shoot multiplication of N. alata. The highest number of shoot was shown in the treatment of 0.5 ml/l BAP, 1/3 MS (A3B2) with the average number 5.50 units. The use of MS medium indicated a statistically significantly different effect on the shoot multiplication of N. alata, with the highest number of shoot was shown in the use of 1/3 MS (B2) mediawith the average number 4,20 units; while the giving of BAP hormone did not indicate a statistically significantly different effect on the shoot multiplication of N. alata, with the highest number of shoot was shown in the use of BAP 0.1 ml/l (A1) with the average number 4.20 units. The conclusion of this research was the best shoot multiplication of N. alata happens in the treatment 0.5 ml/l BAP, 1/3 MS (A3B2); while if it just usedthe MS media the best shoot multiplication was shown in the use of
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Judul: Penentuan Nilai Eigen dan Vektor Eigen dari Matriks Tridiagonal 2-Toeplitz dengan Pendekatan Polinomial Chebyshev
Abstrak: The eigenvalues and eigenvectors of a matrix can be determined by finding its characteristic polynomials. The characteristic polynomials of a tridiagonal 2-Toeplitz matrix is shown to be closely connected to polynomials which satisfy the Chebyshev recurrence relationship. If the order of the matrix is odd, then the eigenvalues are found explicitly in terms of the Chebyshev zeros and the eigenvectors are found in terms of the polynomials satisfying the recurrence relationship. For even ordered matrices, the situation is more complicated. The problem in these cases is that although the Chebyshev recurrence formula is still applied, its initial values are not generating Chebyshev polynomials
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