Judul: Kearifan Lokal Komunitas Kuta Dalam Pengelolaan Sumberdaya Air
Abstrak: Penelitian ini bertujuan untuk menggali dan menganalisis kearifan lokal komunitas Kuta dalam menjaga dan mengelola sumberdaya air; menganalisis peran pemimpin adat dalam proses ajar kearifan lokal; dan menggali strategi nafkah rumah tangga. Data dikumpulkan menggunakan kombinasi pendekatan kuantitatif dan kualitatif. Hasil penelitian menunjukkan, pertama, di komunitas Kuta berkembang norma perlindungan sumberdaya alam, dan norma kehidupan sehari-hari. Melalui budaya pamali (tabu, larangan) norma-norma tersebut mewujud dalam bentuk kearifan lokal perlindungan mata air dan hutan keramat. Kedua, di mata responden Ketua Adat dipandang sebagai pemimpin yang paling besar peranannya dalam memelihara kearifan lokal air dibanding Kuncen, Sesepuh, dan Lurah. Sementara dalam memelihara kearifan lokal hutan. Sesepuh dan Kuncen dipandang berperan lebih besar dibanding Ketua Adat dan Lurah. Secara keseluruhan Kuncen dan Sesepuh dipandang berperan lebih besar dalam memelihara kearifan lokal air dan hutan dibanding Ketua Adat, dan Lurah. Ketiga, dari berbagai luas penguasaan lahan, pendapatan rumah tangga responden dari sektor on farm, off farm, dan non farm dapat dikatakan tidak terlampau timpang. Pendapatan rumah tangga responden dengan luas penguasaan lahan <1 ha rata-rata mencapai Rp 2 juta per rumah tangga per bulan. Sementara pendapatan petani dengan penguasaan lahan >1 ha rata-rata mencapai Rp 3,3 juta per rumah tangga per bulan.
Keyword: kearifan lokal, proses ajar, pemimpin lokal, sumberdaya air, strategi nafkah

Judul: Kearifan Lokal dalam Pengelolaan Sumberdaya Air di Kampung Kuta (Desa Karangpaningal, Kecamatan Tambaksari, Kabupaten Ciamis, Jawa Barat)
Abstrak: Water is important resources to fulfill human needs. So, water resources must managed properly in order to maintain the sustainability of resources. Water resources management should adapt to each area’s characteristic because it has different local wisdom. Local wisdom not only as the features of a community, but also as the effort for ecological environment sustainability. The purpose of this research is to identify the form of local wisdom that existed in Kampung Kuta and identify local wisdom implementation in taking care of water resources sustainability. The method of this research is qualitative approach with descriptive research type. This descriptive research gives clear picture about phenomenon of local wisdom and its application of water resources management. Researcher uses methodologies triangulation that is deep interview, limited of perception shares, and observation. Research data is presented in the form of daily note and presented in direct citation. This research is conduct in the countryside of Karangpaningal, District of Tambaksari, Sub-Province of Ciamis, Province of West Java. The result of this research shows existence of Pamali as the form of local wisdom that defended society in by generations. The form of pamali that become obligatory tradition norm of society that is continuity of tradition house, enjoinment of dead body obsequies, enjoinment of well making, and continuity Hutan Keramat. The culture of pamali as the local wisdom in Kampung Kuta not experiences of transformation. This local wisdom are fixed defended and effective in arranging society life and nature. Pamali are alighted from old generation to the rising generation. Transfers of knowledge are conducted by mouth passed by stories and effective for permanence the local wisdom. Local wisdom implementation in water resources management seen from Pamali that prohibit society to make well. Pamali has given positive impact of water resources sustainability in Kampung Kuta. This condition are proved with the of appreciation Kalpataru in the case of environment sustainability in 2002. Water resources in Kampung Kuta are used in two functions that is to fulfill everyday need and for ritual tradition Nyipuh in Hutan Keramat.
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Judul: Characterization and Expression of Tomato infectious chlorosis virus Coat Protein Gene in Escherichia coli
Abstrak: Tomato infectious chlorosis virus (TICV) is a member of Crinivirus genus. TICV infects tomato crop in Garut and Cipanas, West Java Indonesia. Tomato initially showed bright interveinal yellowing symptoms. The coat protein gene (CP-TICV) was amplified by RT-PCR from total RNA extracted from infected tomato leaves and the amplified fragment was cloned and completely sequenced. The fragment was subsequently subcloned into the pET-21b expression vector. The recombinant plasmid was transformed to Escherichia coli strain BL21(DE3)pLysS to express the coat protein. The coat protein fused to a 6xhistag, was purified by affinity chromatography using a NiNTA spin column. The identity of the purified protein was confirmed by SDS-PAGE. In this experiment, 792 bp of CP-TICV gene of TICV virus has been successfully cloned, sequenced, and expressed in E. coli. Based on nucleotide sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain strain and 99% identity to North America, France, and California strains and based on amino acid sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain, North America, France strains and 99.2% identity to California strain. CP-TICV showed over expressed in E. coli when it is induced with 1 mM IPTG and incubated at 37oC. Purified CP-TICV-Histag recombinants protein sized 29 kDa based on SDSPAGE analysis.
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