Judul: Production of Crude Bacteriocin Lactobacillus plantarum 2C12, 1A5, 1B1, 2B2 from Beef and Their Antimicrobial Activity Against Patogenic Bacteria
Abstrak: Bacteriocin is an antimicrobial peptides produced by lactic acid bacteria which can be a safe biopreservatif agent. The production of bacteriocin have to be done to replace the existence of synthetic preservatives which cause diseases and have been used to decrease the contamination of spoilage and pathogenic bacteria that can be found in many food material. The lactic acid bacteria which used in this research were the isolates of Lactobacillus plantarum 2C12, 1A5, 1B1 and 2B2, that have been isolated from local beef and deposited at Animal Science Faculty of Bogor Agriculture University. This research has been done from February to August 2011. The aims were to learn about the growth curves of L. plantarum 2C12, 1A5, 1B1 and 2B2, to identify the antimicrobial activity of crude bacteriocin from some different isolates lactic acid bacteria and their effects of to some indicator bacteria by diffusion well method agar. The observed variable in this research was the inhibitation zone around the well which refer to the antimicrobial activity from bacteriocin to indicator bacteria and it has been explained by Completely Randomized Design (CRD) with factorial arrangement 5x4. The results of this research showed that all isolates of L. plantarum (2C12, 1A5, 1B1 and 2B2) had some antimicrobial compounds which could decrase the indicator bacteria’s growth, such as Salmonella entritidis ser. Thypimurium ATCC 14028, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Bacillus cereus, dan Staphylococcus aureus ATCC 25923.
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Judul: Produksi Bakteriosin Asal Lactobacillus plantarum sebagai Antimikrob dan Pengujian Ketahanannya terhadap Panas
Abstrak: Bacteriocin is commonly used to biopreservative agent in food. Bacteriocin is an antimicrobial substance that is capable to inhibit toward pathogenic and food borne bacteria. It was reported that indigenous isolate of Lactobacillus plantarum isolated from Indonesian local beef producing an antimicrobial substance (Arief et al., 2008). The objective of this research was to characterize the heat stability of plantaricin at pasteurization and sterilization temperature. The treatments were heat temperature (80 °C for 30 minutes and 121 °C for 15 minutes) and strains of Lactobacillus plantarum (2C12, 1A5, 1B1 and 2B2) usage. The variable was diameter of inhibition zone of antagonistic activity. Antimicrobial activity of plantaricin of Lactobacillus plantarum 2C12, 1B1, 2B2, and 1A5 did not lost after treated at 80 °C for 30 minutes or at 121 °C for 15 minutes. That result showed that plantaricin was stable at pasteurization and sterilization temperature. Plantaricin produced by strains of L. plantarum against indicator bacteria such as Pseudomonas ATCC 27853, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 and Salmonella enteriditis ser. Typhimurium ATCC 14028 had different activity each other. However, inhibition activity of plantaricin did not lead against Bacillus cereus. Stability of antimicrobial activity of plantaricin to inhibit indicator bacteria after heat treatment proved that plantaricin has a potential as food biopreservative.
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Judul: Cycle Hamilton pada Graf Lengkap, Graf Regular, dan Graf 2-Connected 4-Regular Berorder Kurang dari Sepuluh
Abstrak: Suatu graf disebut graf Hamilton apabila graf tersebut memuat cycle Hamilton, yaitu cycle yang melewati setiap vertex tepat satu kali. Menentukan keberadaan cycle Hamilton pada suatu graf dapat menggunakan syarat cukup yang berupa teorema-teorema yang telah diperkenalkan sejak tahun 1950-an. Beberapa syarat cukup yang dibahas dalam karya ilmiah ini adalah syarat cukup yang berhubungan dengan graf lengkap dan graf regular. Suatu graf lengkap berorder 𝑛������ ≥ 3 dan graf r-regular berorder 𝑛������ ≤ 2𝑟������ + 1 merupakan graf Hamilton, Karena kedua graf tersebut memuat cycle Hamilton. Selanjutnya, syarat cukup tersebut digunakan untuk menentukan keberadaan cycle Hamilton pada graf 2-connected 4- regular berorder kurang dari 10.
Keyword: graf Hamilton, graf regular, graf lengkap, graf 2-connected 4-regular