Judul: Profil Komunitas Mikrobial pada Rizosfer Kelapa Sawit Terinfeksi Ganoderma boninense Menggunakan Next Generation Sequencing
Abstrak: Komunitas pada rizosfer menjadi perhatian utama untuk menemukan mikrob potensial sebagai agen pengendali hayati terhadap Ganoderma boninense pada kelapa sawit. Penelitian bertujuan (i) menguraikan komunitas mikrob rizosfer pada tanaman kelapa sawit terinfeksi G. boninense dan tanaman kelapa sawit sehat, (ii) menggali potensi mikrob sebagai agen pengendali hayati. Identifikasi mikrob pada lingkungan ini menggunakan gen 16S rRNA dan region ITS sebagai marker filogenetik dan teknologi Next Generation Sequencing. Sekuensing gen 16S rRNA menghasilkan 94,650 sekuen pada sampel sehat TH1 (Tanah Health 1), 89,137 sekuen pada TH2, 71,585 sekuen pada sampel terinfeksi TD1 (Tanah Disease 1) dan 99,595 sekuen pada TD2. Komunitas bakteri didominasi oleh Actinobacteria dengan kelimpahan relatif 37-44% dari total sekuen gen 16S rRNA setiap sampel. Kelimpahan Saccharopolyspora yang mencapai 12% pada sampel sehat TH1 dan Pilimelia sebesar 3.94% pada sampel TH2 berpotensi untuk dikembangkan menjadi agen pengendali hayati terhadap G. boninense. Komunitas fungi didominasi oleh Ascomycota dengan kelimpahan relatif 11-51% dari total sekuen region ITS setiap sampel. Kelimpahan Talaromyces yang mencapai 18.40% pada sampel sehat (TH2), Gongronella sebesar 3.02% pada TH1 dan Chaetomium sebesar 2.77% pada TH2 berpotensi untuk dikembangkan sebagai agen pengendali hayati G. boninense.
Keyword: G. boninense, komunitas bakteri, komunitas fungi, rizosfer

Judul: Eksplorasi Mikroba Rizosfer dan Hubungannya dengan Sifat Fisik & Kimia Tanah pada Tanaman Kelapa Sawit Terinfeksi Ganoderma boninense Pat.
Abstrak: Oil palm (Elaeis guineensis Jacq.) is one of the plantation crops which has a significance in contributing foreign exchange to the country from non-oil sector. One of the fence in development oil palm cultivation is the presence of stem rot disease caused by Ganoderma boninense. This research aims to explore the microbial rhizosphere surrounding to the oil palm plants, as well as its relationship to the condition of physical and chemical soil characteristics. The study was conducted by taking a surrounding soil of oil palm plantations as a sample and made its suspension. The suspension was made to serial dilutions to 10-8 dilution rate. In vitro antagonism test between antagonist candidate of actinomycetes to G. boninense conducted by using a dual culture methods. Observations were made by measuring the radial growth of pathogenic fungi and actinomycetes isolates were away from fingers pathogenic fungi actinomycetes isolates were approaching. The results of microbial isolation consist of 76.9% fungal isolates, 12.82% bacterial isolates, and 10.26% actinomycetes isolates. The content of chemical characteristics soil at the study was sited at pH 4.43 it is included in a slightly acid soil type and content of C/N ratio 8.78 is low. The results of in vitro experiments showed that isolate AK1 (actinomycetes 1), AK2 (actinomycetes 2), and AK3 (actinomycetes 3) have a significant effect on the growth of pathogenic G. boninense fungi on PDA at the fifth day.
Keyword: Ganoderma boninense, microbial rhizosphere, oil palm

Judul: Dummy Title
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Keyword: dummy