Judul: Landscape Design of Bendi Park as Outdoor  Sports and Play Area
Abstrak: Tingginya jumlah penduduk dengan padatnya bangunan di perkotaan terutama Jakarta menyebabkan tingginya kebutuhan ruang terbuka hijau dalam suatu wilayah. Taman Bendi sebagai salah satu taman di Jakarta memiliki ruang terbuka hijau yang saat ini kondisinya belum optimal dari segi fasilitas dan penggunaannya. Penelitian ini bertujuan membuat desain lanskap Taman Bendi sebagai area olahraga outdoor dan bermain di Kecamatan Kebayoran Lama dan sekitarnya. Penelitian ini dilakukan dengan menggunakan metode kualitatif dan kuantitatif. Metode kualitatif adalah penelitian yang menghasilkan data deskriptif berupa kata-kata tertulis dari orang orang dan perilaku yang dapat diamati. Dalam pendekatan kualitatif fokus terhadap aspek fisik dan biofisik. Metode analisis kuantitatif merupakan penelitian ilmiah yang sistematis terhadap bagian-bagian dan fenomena serta kualitas hubungan hubungannya. Berdasarkan pendekatan kuantitatif, data hasil kuesioner diolah menggunakan metode cross tabulation untuk mengetahui pengaruh rentang usia terhadap persepsi dan preferensi desain. Hasil penelitian ini berupa site plan dengan desain yang dapat memaksimalkan potensi dan memberikan solusi permasalahan yang ada di Taman Bendi, planting plan, gambar potongan, dan gambar perspektif., The high number of residents with the density of buildings in urban areas, especially Jakarta, causes a high need for green open space in an area. Bendi Park as one of the parks in Jakarta has green open space which is currently not optimal in terms of facilities and use. This study aims to design the landscape of Bendi Park as an outdoor sports and play area in Kebayoran Lama District and its surroundings. This research was conducted using qualitative and quantitative methods. Qualitative method is research that produces descriptive data in the form of written words from people and observable behavior. In the qualitative approach the focus is on physical and biophysical aspects. The method of quantitative analysis is a systematic scientific study of the parts and phenomena and the quality of their relationships. Based on the quantitative approach, the questionnaire data were processed using the cross tabulation method to determine the effect of age range on design perceptions and preferences. The results of this study are in the form of a site plan with a design that can maximize potential and provide solutions to problems that exist in Bendi Park, planting plan, section drawings, and perspective pictures.
Keyword: Area olahraga dan bermain, desain lanskap, ruang terbuka hijau, taman  bendi

Judul: Landscape Design of Kebondalem Main Stadium in Kendal Regency Based on Biophilic
Abstrak: Stadion Utama Kebondalem merupakan salah satu ruang terbuka hijau yang ada di Kabupaten Kendal. Kondisi Stadion Utama Kebondalem Kendal saat ini sedang dalam proses rencana pengembangan. Penelitian ini bertujuan untuk menganalisis potensi dan permasalahan dengan pendekatan aspek fisik-biofisik dan sosial-budaya di Stadion Kebondalem Kendal sebagai panduan dalam merancang rekomendasi desain. Metode yang digunakan adalah pendekatan desain berdasarkan persepsi dan preferensi pengguna. Hasil penelitian menunjukkan bahwa tapak memiliki potensi dan kendala. Terutama pada aspek sosial budaya, tapak memiliki nilai persepsi yang kurang baik oleh pengunjung dan dianggap tidak dapat memenuhi kebutuhan aktivitas yang ada. Konsep desain yang diangkat adalah biofilik. Konsep biofilik merupakan bagian dari konsep yang mendekatkan manusia dengan alam secara lebih intim. Selain itu, desain biofilik dapat memberikan dampak bagi pengguna seperti mengurangi tingkat stress, meningkatkan produktivitas, meningkatkan kreatifitas dan kejernihan pikiran, meningkatkan kesejahteraan hingga meningkatkan laju pemulihan tubuh manusia. Desain akhir dibuat dengan pola adopsi sel tanaman dan menciptakan ruang terbuka hijau yang fungsional. Dari desain yang dibuat, menunjukan bahwa Stadion Kebondalem Kendal dengan menerapkan konsep biofilik dan melakukan penataan ruang, setidaknya dapat mewadahi 8 jenis aktivitas. Tidak hanya memiliki fungsi untuk mewadahi aktivitas olahraga, namun juga dengan mewadahi berbagai kegiatan lain dari masyarakat sekitar, seperti relaksasi, apresiasi, komersial, bermain, sosialisasi, edukasi, dan hiburan., Kebondalem Main Stadium is one of the green open spaces in Kendal City. The condition of Kebondalem Kendal Main Stadium is currently in the process of development plan. This research aims to analyze the potential and problems with the approach of physical-biophysical and socio-cultural aspects at Kebondalem Kendal Stadium as a guide in designing design recommendations. The method used is a design approach based on user perceptions and preferences. The results showed that the site has potential and constraints. Especially in the socio-cultural aspect, the site has an unfavorable perception value by visitors and is considered unable to meet the needs of existing activities. The concept raised is biophilic. The biophilic concept is part of a concept that brings humans closer to nature in a more intimate way. In addition, biophilic design can have an impact on users such as reducing stress levels, increasing productivity, increasing creativity and clarity of thought, improving well-being to increasing the rate of recovery of the human body. The final design is made with a plant cell attachment pattern and creates a functional green open space. From the design made, it shows that the Kebondalem Kendal stadium by applying the biophilic concept and structuring the space, can accommodate at least 8 types of activities. not only has a function to accommodate sports activities, but also by accommodating various other activities of the surrounding community, such as relaxation, appreciation, commercial, playing , socialization, education, and entertainment.
Keyword: Desain Lanskap, ruang terbuka hijau, biofilik, stadion

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
Keyword: