Judul: Color Psychology Based Public Park Design for Burnout Prevention in College Students
Abstrak: Burnout merupakan kondisi dimana dapat menyebabkan seseorang untuk merasakan penurunan motivasi belajar dan memunculkan sifat negatif. Kondisi ini rentan dialami oleh seseorang di usia muda karena kurangnya kemampuan dalam menghadapi suatu tekanan. Termasuk pada mahasiswa yang juga memiliki banyak tuntutan tugas. Disamping itu, diketahui bahwa warna memiliki dampak positif terhadap psikologis seseorang. Setiap warna memiliki sifat yang berbeda-beda pada otak manusia. Maka dari itu, elemen desain ini dapat dimanfaatkan menjadi sebuah sarana pencegahan burnout pada mahasiswa. Hal tersebut yang menjadi tujuan dari penelitian ini, yaitu mengetahui preferensi warna pada lanskap yang dapat memberikan dampak positif terhadap burnout pada mahasiswa. Metode yang digunakan merupakan metode deskriptif. Dilakukan kuesioner pada tahap research and analysis untuk mengetahui kondisi burnout pada mahasiswa dan preferensi warna. Dihasilkan kelompok terbanyak adalah mahasiswa dengan kondisi sulit berkonsentrasi dan kehilangan motivasi, Dimana kedua hal ini merupakan ciri-ciri burnout. Berdasarkan hasil kuesioner kepada responden, warna biru merupakan warna yang memiliki nilai tertinggi untuk 4 aspek yang memberikan dampak positif terhadap kondisi burnout pada mahasiswa. Maka dari itu penggunaan warna biru didominasi pada desain taman.
Keyword: burnout, psikologi warna, taman publik

Judul: Artwork Design as Supporting Aspect for Healing Garden at Academic Event Plaza, IPB University
Abstrak: Academic Event Plaza sebagai taman yang utamanya digunakan untuk berbagai kegiatan kampus, juga menjadi pilihan mahasiswa untuk menghilangkan academic burnout atau biasa disebut proses healing. Academic Event Plaza memiliki tiga fungsi, yaitu pendidikan, sosialisasi, serta rekreasi yang mencakup fungsi healing. Kebutuhan pengguna tapak akan tempat untuk healing dari academic burnout dapat ditunjang dengan penempatan artwork interaktif. Tujuan dari penelitan ini adalah mengidentifikasi dan menganalisis kondisi eksisting pada tapak, menyusun konsep artwork yang sesuai untuk Academic Event Plaza, dan, membuat desain artwork sebagai penunjang healing garden di Academic Event Plaza, Institut Pertanian Bogor. Penelitian ini dilaksanakan menggunakan metode deskriptif melalui empat tahap yang mencakup pengumpulan data, analisis dan sintesis, desain konsep, dan gambar konstruksional. Konsep dasar dalam penelitian ini adalah penggunaan artwork berbasis art-therapy untuk menunjang healing garden. Konsep desain dari penelitian ini adalah merupakan pengembangan dari metode art-therapy, yaitu multi-sensory, dimana bentuk artwork akan mewakili kelima indra manusia. Yaitu Artwork Sight yang mewakili indra penglihatan, Artwork Sound yang mewakili indra pendengaran, Artwork Smell yang mewakili indra penciuman, Artwork Taste yang mewakili indra perasa, dan Artwork Touch yang mewakili indra peraba, menjadi satu-kesatuan (unity) pengalaman artistik bagi pengguna., Academic Event Plaza is a university park at IPB University, mainly used for various campus activities, and also an option for students to reduce academic burnout, commonly known as a way of healing from stress. Academic Event Plaza as a park has three main functions, which are education, socialization, and recreation function which includes a function of healing. Interactive artwork installation can support the park’s function to be a place for users in need of a place to heal from academic burnout. The purpose of this research is to identify and analyze the existing conditions on the site, make a suitable artwork concept for Academic Event Plaza, and to design artworks to support healing garden at Academic Event Plaza, IPB University. This research uses descriptive method, broken down into four stages which include data collecting, analysis and synthesis, concept design, and constructional drawing. The basic concept in this research is the use of art-therapy-based artwork to support the healing garden. The design concept of this research is the development of the art-therapy method, multi-sensory, where the artwork will represent five human senses. The artworks will include 5 parts, which are ‘Sight’ artwork representing the sense of sight, ‘Sound’ artwork representing the sense of hearing, ‘Smell’ artwork representing the sense of smell, ‘Taste’ artwork representing the sense of taste, and ‘Touch’ artwork representing the sense of touch, all of them forming a unity for the users to experience fully.
Keyword: art therapy, artwork, academic event plaza, healing garden, ipb university

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable