Judul: Analisis kanonik hubunga indikator kependudukan dengan derajat kesehatan
Abstrak: Bernat Simbolon. Analisis Kanonik Hubungan Indikator Kependudukan dengan Derajat Keseliaran ( di bawah bimbingan Bambang Sumantri dan Abdurrauf Rambe). Penelitian ini berujuan utrtuk menggambarkan tingkat kekuatan hubungan beberapa indikator kependudukan terhadap derajat kesehatan dan mengelompokkan propinsi di Indoriesia berdasarkan kumpulan peubah tersebut. Fakror-fakror yang mampu memberi irformasi dasar mengenai indikator kependudukan adalah kesehatan, gizi, pendidikan, ckononri, sosial budaya dun lingkungan sosial. Sedangkan derajat kesehatan secara kuantitatifdiukur dari angka kematian bayi, angka kematian balita dan harapan hidup masyarakat (laki-laki dan wanita).
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Judul: Pengelompokan Propinsi Di Indonesia Berdasarkan Beberapa Indikator Kesehatan
Abstrak: Pembangunan kesehatan memiliki tujuan UIltuk meningkatkan kesehatan masyarakat. Walaupun tclah banyak kemajuan yang tclah dicapai dalam bidang kesehatan, tetapi masih ada sebagian masyarakat Indonesia yang masih sulit untuk mendapatkan pelayanan kesehatan. Penelitian dilakukan wltuk lllengelolllpokkan propinsi di Indonesia berdasarkan beberapa indikator kesehatan dalam upaya mempcnTIudah mencapai Indonesia sehat 2010. Analisis gerombol dilakukan dua tahap, dengan mctode Gerombol Hirarki Pautan Rataan dan ukuran jarak Euclid. Analisis gerombol tahap pel1allla menghasilkan tiga gerombol. Gerombol II dan III cenderung lllelllencil dibanding gerombol lainn),a. Analisis tahap kedua dilakukan dengan menggerolllbolkan propinsi-propinsi pada geromboll. Pada tahap ini dihasilkan empat gerombol. Propinsi Nusatenggara Barat dan Timur, Kalimantan Barat, Maluku dan Aceh memiliki tingkat kesehatan yang relatif rendah, Ulltuk itu propinsi-propinsi tcrsebut sebaiknya memperoleh perhatian yang lebih intensif dalam bidang kesehatan. Sedangkan propinsi Jawa-Bali tingkat kesehatannya relatif tinggi.
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Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable