Judul: Hubungan Kebiasaan Tidur dan Menonton Televisi dengan Status Gizi Remaja di SMP Bina Insani Bogor
Abstrak: In Indonesia, especially big cities, there has been lifestyle changing of adolescents who commonly tend to be sedentary physical activities. One of the sedentary physical activities that affect body mass index (BMI) is watching television. In addition, sleeping habits also influence adolescents BMI. The purpose of the present study was to determine the relationship between sleeping and watching television habits on adolescents and BMI. This study used a cross-sectional study design with purposive sampling. Samples consisted of 48 adolescents aged 13–15 years who were junior high school student at Bina Insani Bogor School. The results showed a significant relationship between adolescents BMI with gender (p = 0.01), father's education (p = 0.04), night sleep duration (p = 0.01) and the consumption of snacks while watching television (p = 0.03). However, there was no significant relationship between adolescents BMI with age (p = 0.26), maternal education (p = 0.05), level of physical activities (p = 0.77), night sleep time (p = 0.75), and duration of watching television (p = 0.93).
Keyword: watching television, sleeping, body mass index, adolescent

Judul: Hubungan Citra Tubuh, Tipe Perilaku Makan, dan Gaya Hidup dengan Status Gizi Siswa SMA Negeri 1 Bogor
Abstrak: Penelitian ini bertujuan menganalisis hubungan citra tubuh, tipe perilaku makan, dan gaya hidup (durasi tidur, kebiasaan sarapan, dan aktivitas fisik) dengan status gizi siswa SMA Negeri 1 Bogor. Penelitian ini bersifat cross sectional study dengan subjek sebanyak 66 siswa pria. Data karakteristik subjek dan keluarga serta gaya hidup dikumpulkan dengan pengisian kuesioner. Citra tubuh diukur menggunakan FRS (Figure Rating Scale), sedangkan tipe perilaku makan diukur menggunakan DEBQ (Dutch Eating Behavior Questionarre). Sebesar 46.97% subjek memiliki citra tubuh negatif, 53% memiliki perilaku external eating, 92.42% memiliki durasi tidur yang kurang, 75.76% memiliki aktivitas fisik yang ringan (PAL: 1.40-1.69), dan 16.67% tidak rutin sarapan (<4 kali/minggu). Tidak ada hubungan signifikan (p>0.05) antara perilaku external eating dan durasi tidur dengan status gizi, begitupula antara tipe perilaku makan dengan citra tubuh. Terdapat hubungan negatif signifikan (p<0.05) antara citra tubuh, tipe perilaku emotional eating, kebiasaan sarapan, serta aktivitas fisik dengan status gizi, sedangkan ada hubungan positif yang signifikan antara tipe perilaku restrained eating dengan status gizi. Penelitian ini menunjukkan bahwa semakin positif citra tubuh, semakin cenderung berperilaku emotional eating, semakin rutin sarapan, dan semakin berat aktivitas fisik, maka status gizi semakin normal. Sedangkan, semakin cenderung berperilaku restrained eating, semakin berhubungan dengan status gizi lebih.
Keyword: citra tubuh, gaya hidup, perilaku makan, remaja pria, status gizi

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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