Judul: Identification and Total of Lactic Acid Fermentative Bacteria in Primate Feces
Abstrak: Bakteri asam laktat (BAL) bermanfaat bagi kesehatan, terutama peranannya sebagai probiotik dan antimikroba dalam pengobatan penyakit saluran pencernaan pada primata. Penelitian ini bertujuan untuk mengidentifikasi serta menghitung total koloni bakteri asam laktat pada feses lima jenis primata yaitu orang utan, owaungko hitam, owa ungko coklat, siamang, dan lutung yang dikoleksi dari Kebun Binatang Bukittinggi. Sampel dilakukan pengenceran 10-1 hingga ke 10-7 dandiinokulasikan ke dalam media selektif MRSA (de Man Rogosa Sharpe Agar) dengan metode pour plate. Koloni bakteri asam laktat yang tumbuh pada media diamati dan dihitung total koloninya. Koloni terpilih diisolasi untuk selanjutnya diidentifikasi menggunakan beberapa uji biokimia yaitu uji katalase, uji oksidase, uji Sulfide Indol Motility, uji Methyl Red-Voges Proskauer, dan uji fermentasi karbohidrat. Penelitian berhasil mengisolasi bakteri dari genus Lactobacillus, Leuconostoc, dan Weissella. Hasil penelitian menunjukkan orang utan menghasilkan total bakteri asam laktat tertinggi yaitu sebesar 3,1 x 108 CFU/ml., Lactic acid bacteria (LAB) are beneficial for health, especially their role as probiotics and antimicrobials in the treatment of digestive tract diseases in primates. This research aims to identify and count the total colonies of lactic acid bacteria in the feces of five primate species, namely orangutans, black gibbons, brown gibbons, siamang, and langurs collected from the Bukittinggi Zoo. Samples were diluted 10- 1 to 10-7 and inoculated into MRSA (de Man Rogosa Sharpe Agar) selective media using the pour plate method. Colonies of lactic acid bacteria growing on the media were observed and the total colonies were counted. Selected colonies were isolated for further identification using several biochemical tests, namely catalase test, oxidase test, Sulfide Indole Motility test, Methyl Red-Voges Proskauer test, and the carbohydrate fermentation test. The results of the research succeeded in isolating lactic acid bacteria from the genera Lactobacillus, Leuconostoc, and Weissella. Orangutans produced the highest total lactic acid bacteria, which was 3.1 x 108 CFU/ml.
Keyword: lactic acid bacteria, primates, total plate count

Judul: Identification and Total of Lactic Acid  Fermentative Bacteria in Sumatran Orangutan (Pongo abelii)
Abstrak: Bakteri asam laktat (BAL) merupakan bakteri yang digolongkan ke dalam bakteri yang bermanfaat bagi kesehatan inang, terutama menjaga keseimbangan flora di dalam pencernaan. Penelitian ini bertujuan melakukan isolasi dan identifikasi jenis bakteri asam laktat serta mengetahui jumlah koloninya pada feses orang utan sumatra. Sampel dilakukan pengenceran 10-1 hingga ke 10-8, kemudian diisolasikan ke dalam media selektif MRS agar menggunakan metode pour plate. Setelah diinkubasi, koloni yang tumbuh pada media diamati dan dihitung total koloni bakteri asam laktat. Koloni yang terpilih dilanjutkan untuk diisolasi dan diidentifikasi menggunakan uji sederhana, yaitu uji biokimia dengan uji katalase, uji oksidase, uji Sulfide Indole Motility, uji Methyl Red-Voges Proskauer, dan uji fermentasi gula-gula. Bakteri asam laktat yang dapat diidentifikasi dari sampel feses orang utan sumatra adalah Leuconostoc sp., Weissella sp., Lactobacillus sp. Hasil penelitian menunjukkan total bakteri asam laktat pada sampel sebesar 8,98 x 106 CFU/ml., Lactic acid bacteria (LAB) is a type of bacteria which belong to have beneficial health for the host, especially to maintain microflora stability of the digestion. This research aimed to isolate and identify the lactic acid bacteria in the fecal of sumatran orangutan. Samples were dilution 10-1 to 10-8, then isolated in selected media MRSA using the pour plate method. After the incubation, separated colonies which grown in media were observed and were counted as a total colony of lactic acid bacteria. The selected colony continued to isolate and identify using simple tests, such as biochemical test with catalase test, oxidase test, Sulfide Indole Motility (SIM) tests, Proskauer's Methyl Red-Voges test, and carbohydrate fermentation test. Lactic acid bacteria that can be identified from fecal samples of sumatran orangutan are Leuconostoc sp., Weissella sp., dan Lactobacillus sp. The results showed that the total lactic acid bacteria in the sample was 8,98 x 106 CFU/ml.
Keyword: lactic acid bacteria, fecal sumatran orangutan, total plate count

Judul: Thermal Characteristics and Dynamics of Tropical Cyclone over Western North Pacific Ocean: A Case Study of Typhoon Songda (Chedeng) 2011
Abstrak: The frequent occasion of Tropical Cyclone (TC) over Western North Pacific (WNP) Ocean lies from 10˚N to 26˚N and 121˚E to 170˚E. Sources of TC development come from warm ocean temperature where latent heat is released during condensation processes and subsequently concentrated in the boundary layer. Relative humidity at 850-700 hPa is responsible to produce the most intense storm. Meanwhile, weak vertical wind shear between 850-200 hPa is one of the dynamical factors that lead to the development of TC. Pressure level of 850-200 hPa is chosen to minimize the thermodynamic effect in the lower troposphere. Typhoon Songda (Chedeng in the Philippines) or TSC lasted from May 18th to 30th, 2011 and strengthened into powerful Super Typhoon stage between May 24th and 27th. Long curving track and the exhibition of all sequences of TC life cycle, TSC is then considered as an ideal case of TC. The objective of the study with case of TSC is to investigate the role of thermal characteristics and dynamics on TC intensity. The research is carried out by using Advanced Research of Weather Research and Forecasting model (WRF ARW model) version 3.3. Two domains are employed in the model. The first domain situate from 02˚N to 40˚N and 118˚E to 149˚E and cover spatial resolution () of 33.1 km. The second domain situate from 09˚N to 27˚N and 119˚E to 132˚E with of 11 km. Timestep of the model () is 120 seconds. The model is supported by the following physical parameterization, i.e., Yonsei University Scheme (YSU) in the boundary layer, Kain-Fritsch scheme (KF) in the cumulus, and WRF Single-Moment 3-class scheme in the microphysics. Initial meteorological condition prior to the formation of the cyclone is generated using WRF ARW standard-release model. Sensitivity of the model output is obtained from a selected single-parameter change scenarios, namely, 10 % to 20 % decrease and increase of wind shear initial data at 850-200 hPa, and by 10 % to 20 % decrease of relative humidity initial data at 850-700 hPa. The result showed that the negative correlations between wind shear and intensity are found in wind shear scenarios. Wind shears of 10 ms-1 which lead to strengthening of the wind intensity in the early period of Typhoon development are found in wind shear scenarios. The effect of wind shear changes on wind intensity is statistically significant in the 20 % wind shear scenarios. During mature stage, weak wind intensity occurred when wind shear increase as given in the WRF standard-release and the 20 % decreasing scenario. Different situation is found in the 20 % increasing scenario where wind shear tend to weak and lead to strong wind intensity at this stage. Meanwhile, the reduction of moisture supply at 850-700 hPa resulting from 20 % decreasing relative humidity play a major role in the weakening of wind intensity and reduces mechanical energy of the cycle process as much as 300 J kg-1.
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