Judul: Provision of vaccine effectiveness against Ichthyophthirius multifiliis common carp (Cyprinus carpio L) at different temperatures of media
Abstrak: The aims of this study were to assess the physiological and haematological responses of fish due to different water temperatures, Ichthyophthirius multifiliis (ich) infection, and vaccination with ich theront stadia. The research was conducted in three stages. The first stage was designed to determine the temperature range that could cause stress in fish and had potency to cause ich infection. In the second stage, fish was infected with ich. The last stage was carried out to investigate the effect of vaccination. Physiological changes in fish from each stage were determined. The results of the first stage showed that cortisol levels at 20, 24, and 28⁰C and glucose levels at 24 and 28oC increased until 21st day. Fish that was kept at 24oC had the lowest survival rate (87±3.35%) and fish that was kept at 32oC had the highest survival rate (100%). In the second stage, infected fish at 20oC was under great stress, which was characterized by the increase in cortisol levels by 51.38% on 3rd day, increase in glucose levels by 60.93%, decrease in osmolarity by 18.40%, decrease lymphocytes percentage by 16.44%, and the lowest survival rate (58.11±2.16%). The damage that was affected by I. multifiliis could be seen by the presence of hypercellular, many goblet cells, and infiltration of lymphocytes cells. Lastly, vaccination caused the decrease in cortisol levels and increase in glucose levels until 14th day. The prevalence of I. multifiliis in the vaccinated fish decreased with the increase in temperature, and I. multifiliis prevalence at 20⁰C was 80.03±10.00% and at 28⁰C was 0%, and the highest survival rate was obtained at 28⁰C (100%).
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Judul: Efektivitas vaksin DNA anti-KHV pada benih ikan mas Cyprinus carpio melalui metode perendaman dan perlakuan hiperosmotik
Abstrak: Common carp production has decreased since the mass deaths caused by disease of koi herpesvirus (KHV). Therefore, safe technique is needed and can be applied in bulk with cost efficient. The purpose of this research was verified influence of DNA vaccine anti-KHV with dose of 1,3 x 108 cfu / mL was performed through immersion in different duration after hyperosmotic treatment for 2 minutes treatment to produced the resistence common carp. There were six treatments with three replications, A: vaccination for 60 minutes immersion, B: 90 minutes, C: 120 minutes, positive control: fish were not vaccinated and infected with KHV, negative control: fish not vaccinated and were not infected with KHV, control bacteria: fishes that soaked with bacteria did not containing vaccine. The results showed that vaccination for 60 minutes giving highest relative survival (93.55%). Thus, vaccination by immersion and hyperosmotic treatment effectively improve the survival and relative percent survival of fish, and this method can be an alternative in the early control of KHV infection in common carp and koi.
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Judul: Proses Perancangan Lanskap Kawasan Rasuna Epicentrum, Kuningan Jakarta di PT Bakrie Swasakti Utama
Abstrak: Setiap waktu dari tahun ke tahun terlihat adanya berubah fisik pada kota Jakarta. Hal ini disebabkan karena adanya peningkatan jumlah penduduk di Jakarta sehingga menjadikan daya tarik bagi perusahaan-perusahaan pengembang yang bergerak dibidang pengembangan kawasan pada suatu perkotaan untuk menghadirkan suatu kawasan baru dengan sarana dan prasarana yang berada di sekitarnya seperti pusat perbelanjaan, gedung perkantoran, rumah susun (apartemen), fasilitas pendidikan dan lain-lain. Salah satu perusahaan pengembang yang berfokuskan kepada suatu kawasan perkotaan adalah PT Bakrie Swasakti Utama. PT Bakrie Swasakti Utama merupakan anak perusahaan Bakrieland Development Tbk, perusahaan ini telah menyelesaikan proyek Wisma Bakrie 1 dan 2, Graha Capital, dan saat ini sedang melaksanakan proyek pengembangan kawasan perkotaan di bidang properti yaitu berada di Jakarta Selatan tepatnya di daerah Kuningan dengan proyek pembuatan suatu kawasan bisnis yang bernama Kawasan Rasuna Epicentrum. Rasuna Epicentrum adalah kawasan superblok terbesar di Jakarta Selatan dengan luasan mencapai 53,5 Ha dan akan berkembang menjadi 70 Ha, Kawasan ini merupakan suatu proyek dari Bakrieland yang terletak di wilayah Kuningan, Jakarta. Kawasan Rasuna Epicentrum dirancang dengan konsep mixed-use area yang bergaya arsitektur modern yang selaras dengan lanskap di sekitarnya. Penataan lanskap yang juga mengarah kepada taraf internasional dengan menghadirkan satu ciri khas yang dapat menjadi sebuah keunggulan tersendiri bagi kawasan. Hal ini yang melatarbelakangi mahasiswa untuk magang di PT Bakrie Swasakti Utama.
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