Judul: Teknik Pemeliharaan dan Tingkat Kesejahteraan Macan Tutul Jawa (Panthera pardus melas Cuvier, 1809) di Taman Satwa Cikembulan Garut.
Abstrak: Macan tutul jawa merupakan salah satu satwa endemik Pulau Jawa dan terancam punah. Salah satu upaya untuk mempertahankannya yaitu melalui konservasi eksitu, salah satunya di Taman Satwa Cikembulan. Penelitian ini bertujuan mengkaji teknik pemeliharaan dan tingkat kesejahteraan macan tutul jawa di Taman Satwa Cikembulan. Capaian implementasi kesejahteraan satwa diperoleh dengan menggunakan metode PKBSI (Persatuan Kebun Binatang Seluruh Indonesia). Teknik pemeliharaan termasuk aspek perkandangan belum sesuai walaupun ukuran kandang telah memenuhi ukuran minimum, pakan sudah mencukupi, kesehatan baik walaupun ketersediaan fasilitas medis masih kurang, reproduksi sudah baik dengan lahirnya anakan, dan adaptasi sudah baik. Tingkat kesejahteraan macan tutul jawa menurut pengelola 80.60% dengan kategori sangat baik sedangkan menurut pengamat yaitu 65.11% dengan kategori cukup.
Keyword: kesejahteraan satwa, macan tutul jawa, pemeliharaan, Taman Satwa Cikembulan

Judul: Infeksi Cacing Gastrointestinal Pada Macan Tutul Jawa (Panthera Pardus Melas) Di Pusat Penyelamatan Satwa Cikananga Dan Situgunung Park
Abstrak: Macan tutul jawa (Panthera pardus melas Cuvier, 1809) merupakan satwa karnivora endemik Indonesia yang hanya terdapat di Pulau Jawa. Spesies ini dinyatakan terancam punah, sehingga tindakan konservasi diperlukan bagi Macan tutul jawa. Penelitian ini bertujuan untuk mengidentifikasi dan menghitung persentase serta derajat infeksi cacing parasit gastrointestinal pada Macan tutul jawa di lembaga konservasi ex-situ di Pusat Penyelamatan Satwa Cikananga (PPSC) dan kawasan in situ di Situgunung Park, Taman Nasional Gunung Gede- Pangrango (TNGGP). Sebanyak 4 sampel tinja dikumpulkan dari PPSC pada bulan Januari-Februari 2014 dan 3 sampel tinja dari Situgunung Park pada bulan Mei 2013 dan Maret-Juni 2014. Analisis sampel tinja dilakukan dengan menggunakan metode modifikasi McMaster, flotasi sederhana, dan penyaringan bertingkat. Hasil penelitian menunjukkan bahwa seluruh sampel terinfeksi cacing gastrro-intestinal. Jenis cacing yang ditemukan beserta masing-masing persentase infeksinya adalah strongylid (85.71%), diikuti oleh trichurid dan ascarid (57.14%) serta Strongyloides (28,57%) dengan persentase terendah. Kisaran derajat infeksi kecacingan pada Macan tutul jawa di PPSC adalah <50–8825 telur per gram tinja (TTGT). Jumlah TTGT tertinggi pada strongylid (<50-8825 TTGT) di PPSC, diikuti oleh trichurid (<50–3400 TTGT) di PPSC dan Strongyloides (700-1100 TTGT) di SGP. Jumlah TTGT terendah pada ascarid (<50 TTGT) yang ditemukan di PPSC dan SGP.
Keyword: cacing gastrointestinal, Macan tutul jawa, TTGT

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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