Judul: Analisis Keragaman Genetik Temulawak (Curcuma xanthorrhiza Roxb.) dengan Menggunakan Penanda Amplified Fragment Lenght Polymorphism (AFLP)
Abstrak: Indonesia is known by its diversities, especially in herb-medicinal plants. Curcuma xanthorrhiza Roxb. is one of the potential medicinal plant belonging to Zingiberaceae family. This study was to determine genetic diversity of 32 accession Curcuma xanthorrhiza Roxb. Total DNA was extracted from leaf using Sodium Dodesyl Sulphate (SDS) modification methode. Amplified fragment length polymorphism (AFLP) was carried out according to the protocol described in AFLPTM plant mapping kit (PE Applied Biosystem) and the final polymerase chain reaction (PCR) products were separated using The Agilent 2100 Bioanalyzer. The number of fragment produced by 12 pairs primer combination of AFLP ranged from 42 to 60 with and average 0f 52. Data obtained was analyzed by the NTSys program. From the AFLP amplification on 32 DNA samples, it was proven that the accession of Curcuma xanthorrhiza Roxb. had a high degree of diversity. Based on analysis of AFLP and unweighted pair group with arithme average (UPGMA) it was shown that the accession of Curcuma xanthorrhiza Roxb. could be grouped into two cluster at relative ecludian distance of 0.10 (10%). Cluster I for accession from Palembang, Pacitan and Ciamis 2. Cluster II for accession from Makale, Pontianak, Kulonprogo, Mataram, Boyolali, Salatiga, Sumberejo, Bali, P. Seram, Sentolo, Purworejo, Samas Bantul, Ciamis1, Blora, Semarang, Poso, Kalsel, Tagari, Merapi Farm, Salakaria, NTB, Menoreh, Karang Anyar, Mangunan, Medan, Toraja, dan Solok.
Keyword: 

Judul: Analisis Keragaman Genetik Kunyit (Curcuma longa Linn.) Dan Temulawak (Curcuma xanthorrhiza Roxb.) Budidaya Tanah Jawa Berdasarkan Penanda Molekuler RAPD
Abstrak: Penanda molekuler RAPD (Random Amplified Polymorphic DNA) merupakan salah satu teknik yang digunakan dalam analisis keragaman genetik tanaman. Kunyit (Curcuma longa Linn.) dan temulawak (Curcuma xanthorrhiza Roxb.) sebagai tanaman obat herbal potensial memiliki distribusi luas di wilayah Indonesia yang dapat berpengaruh terhadap pola keragaman genetiknya. Penelitian ini bertujuan untuk mengetahui pola keragaman genetik dari 12 sampel genus Curcuma yang berasal dari berbagai daerah di Pulau Jawa. Penelitian diawali dengan optimasi isolasi DNA dan amplifikasi DNA, hingga analisis lokus dan konstruksi pohon filogenetik menggunakan metode UPGMA (Unweighted Pair Group Method Arithmatic Mean) dari software NTSYS. Modifikasi protokol isolasi DNA dengan penambahan Proteinase K setelah proses inkubasi dan RNAse setelah proses pencucian menunjukkan hasil paling baik. DNA yang diisolasi memiliki ukuran berkisar pada 9000 bp dengan kualitas pita yang cukup baik. Amplifikasi DNA dengan 20 primer acak terpilih pada 7 sampel kunyit menghasilkan 278 pita DNA dari 177 lokus, dan pada 5 sampel temulawak menghasilkan 184 pita DNA dari 143 lokus yang seluruhnya merupakan pita polimorfik. Analisis kemiripan genetik 7 varietas Curcuma longa Linn. menunjukkan nilai koefisien kemiripan genetik sebesar 42.37% - 94.35% dan 19.58% - 85.31% pada 5 varietas Curcuma xanthorrhhiza Roxb. Hasil pengelompokan pada nilai koefisien kemiripan genetik 51.65% dan 33.17% menunjukkan bahwa 7 varietas kunyit dan 5 varietas temulawak yang diteliti berasal dari dua kelompok besar.
Keyword: 

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
Keyword: