Judul: Analisis Molekuler dan Fenotipe Galur Padi Transgenik IR64 Generasi T3 yang Mengandung Penanda Aktivasi untuk Toleransi Kekeringan
Abstrak: Activation tagging transgenic rice cv. IR64 had been developed by introducing a construct of activation tagging transposon Ac/Ds. Phenotipic character associated with drought stress tolerance of the transgenic rice lines have not been analyzed. This study aimed to evaluate T3 generation of activation tagging transgenic rice lines cv. IR64 to drought stress treatment. The methodology of study consisted of several steps including germination of samples seeds, screening the seedling with drought strees treatment, molecular analysis of the rice lines identify hpt and bar genes, and observation of agronomic character. Results of study showed that there were 34 out of 84 plants that tolerant to drought stress. A total of 23 plants of tolerant plants were detected carrying only bar gene after PCR analysis and indicated that the plants were stable mutants. Generaly, compared to the non-transgenic plants, there were changes in several agronomic parameters of transgenic rice plants cv. IR64 T3 generation. A total of nine plants increased of its height, six plants had a faster harvesting time, and 21 plants had higher weight of 100 seeds compared by non-transgenic plants.
Keyword: Bogor Agricultural University (IPB), drought stress tolerance, activation tagged, Rice (Oryza sativa L.)

Judul: Karakterisasi Molekuler Tanaman Padi Nipponbare Transgenik 35S::OsERA1 yang Toleran Kekeringan
Abstrak: Drought is one of the factors that resulted in the growth and development of rice plants inhibited. One way to overcome this problem is use of transgenic rice plants that are tolerant to drought. The success of the transformation using OsERA1 gene in Nipponbare genotype rice requires further confirmation of tolerance to drought stress. The aim of this study was to obtain transgenic Nipponbare genotype rice contain gene 35S::OsERA1 drought tolerant, know the number of gene copies, and compare the contents and empty seeds with control. The evaluation of drought the tolerance was performed using Standard System Evaluation for Rice method (IRRI 1996). All tested transgenic genotypes were tolerant to drought. Molecular characterization using southern hybridization showed that transgenic rice plants had 1-3 copies of transgen. Drought stress inhibited plant growth, which lowers the amount of seed and increasing the void content. 18.5.4 and 6.4.10 genotypes are considered tolerant to drouht. 18.5.4 genotype have the number of grains per panicle contents of 36 seeds and 6.4.10 genotype have a 38.03% percentage of empty seeds
Keyword: transgenic plant, drought, southern hybridization, OsERA1 gene

Judul: Karakteristik Anatomi Skelet Kepala Buaya senyulong (Tomistoma schlegelii).
Abstrak: Buaya sepit atau senyulong (Tomistoma schlegelii) merupakan jenis buaya asli Indonesia yang berhabitat di sungai-sungai Sumatera, Kalimantan, dan juga pedalaman Sulawesi. Salah satu ciri khas buaya senyulong adalah bentuk moncong yang runcing dan sempit, namun hingga saat ini informasi mengenai struktur skelet kepala buaya senyulong sangat terbatas. Penelitian ini bertujuan untuk mempelajari karakteristik anatomi skelet kepala buaya senyulong yang dikatikan dengan fungsi dan perilakunya. Penelitian dilakukan secara eksploratif dengan melakukan pengamatan, pencatatan, dan pengambilan gambar dari skelet kepala buaya senyulong. Skelet kepala berukuran panjang 70 cm, lebar 29 cm, dan tingggi 17 cm dengan permukaan kasar pada os nasale, os frontale, os maxilla, os mandibula, os lacrimale, os jugale. Jumlah gigi pada buaya ini sebanyak 78 buah, terdiri dari 4 buah gigi premaxila, 16 buah gigi maxila, dan 19 buah gigi mandibula. Pada regio orbitalis ditemukan dua pasang fenestrae temporales yang berkembang baik. Fenestra supratemporale merupakan lubang oval yang membentang di sepanjang os parietale. Fenestra infratemporale berbentuk segitiga dan dibatasi oleh os squamosum, os postorbitale, os jugulare, dan os quadratum. Os mandibula hewan ini mempunyai symphysis mandibularis yang panjang yaitu garis yang melintang di corpus mandibulae. Secara fungsional ukuran symphysis mandibularis berkaitan dengan adaptasi hewan terhadap diet makan.
Keyword: kepala, moncong, Tomistoma schlegelii