Judul: Morphometry and Genetics Diversity of Wild Silk Moth Cricula trifenestrata Helfer (Lepidoptera: Saturniidae).
Abstrak: The study was conducted to describe morfometry, life cycle, fibroin and cytohcrome C oxidase subunit I (COI) gene characteristics of Cricula trifenestrata. Morphometry data were obtained from 3 locations at Campus IPB Darmaga area, namely in the parking area Graha Widya Graduation (GWW), Bara Tomb Complex, and Animal House C Fapet. Observations were done on the duration and timing of life cycle, viability, morphology, anatomy, and nine morphological traits, namely cocoon weight (CW), shell cocoon weight (SCW), cocoon lenght (CL) cocoon width (CWD), pupae weight (PW), pupae length (PL), pupae width (PWD), thorax length (TL), and wingspan (WS). Data were analyzed descriptively, and discriminant analysis were also performed. Molecular data obtained from genomic DNA extracted from silk glands of larvae. Genomic DNA was amplified and sequenced, and then characterized in order to describe the number and type of nucleotide, the number and types of amino acids on proteins fibroin and COI. Characterization results was compared with fibroin and COI genes sequences of other silk moth available in GenBank and using it to constructed phylogenetic tree. The results showed that C. trifenestrata spend 51.0 ± 7.3 days for one life cycle. All morphological traits significantly affected the grouping by location in each stage of development. The prediction accuracy for the parameter grouping cocoon, pupae and imago were 93.6%, 83.3%, and 92.7% respectively, with the biggest differentiator: CV, PW, and WS respectively. The partial fibroin gene consists of first exon coding region with 42 nucleotides (14 amino acids), an introns (113 nucleotides) and partial coding region of second exon with 831 nucleotides (277 amino acids) long. The comparison with other silk moth showed that fibroin gene were conserved on C. trifenestrata, but very diverse with other species. Nucleotides 25th (C), 35th (T) of the first exon, and 12th amino acids (valine) were diagnostic for the species C. trifenestrata. Nucleotide of second exon encoding 277 amino acids that were dominated by the amino acid alanine (27.8%) and glycine (21.66%). Nucleotide introns were very diverse, more than 70% nucleotide of A and T. The partial COI gene of C. trifenestrata size of 595 nucleotides, encoded 198 amino acids. There was no difference nucleotide sequence of intra species C. trifenestrata, but showed 23.7% diversity with other silk moth. Nucleotide dominated by thymine and adenine bases (± 70%). There are 25 nucleotide diagnostic for C. trifenestrata. Sequenced of 198 amino acids, showed only 10% variation between species of silk moth. Amino acid 107th (valine) and 138th (treinonin) are amino acid diagnostic for C. trifenestrata.
Keyword: Cricula trifenestrata, Fibroin gene, Beneficial insect, Antheraea mylitta

Judul: Morphometrics of Eri Silkworm Cocoon (Samia cynthia ricini) Reared on Different Mountages
Abstrak: Samia cynthia ricini (Lepidoptra: Saturniidae) merupakan ulat sutra non-murbei yang telah didomestikasi dan seratnya sudah banyak dimanfaatkan dalam industri tekstil. Dalam budidaya ulat sutra Eri terdapat berbagai macam jenis media pengokonan yang dapat digunakan. Ukuran morfometrik dan keberhasilan mengokon merupakan indikator yang perlu diperhatikan untuk menentukan tingkat produksi kokon. Tujuan dari penelitian ini adalah menganalisis pengaruh media pengokonan yang berbeda terhadap morfometrik kokon ulat sutra Eri serta persentase keberhasilan mengokonnya. Penelitian ini menggunakan tiga macam perlakuan, yaitu pengokonan dengan media pengokonan klaras, kertas koran yang digulung, serta kotak pengokonan kayu. Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap dengan tiga kali pengulangan. Dalam penelitian ini digunakan sebanyak 270 ekor ulat sutra instar V. Kokon yang diukur diambil secara acak sebanyak 20 buah dari setiap tempat pengokonan. Data dianalisis menggunakan Analysis of Variance (ANOVA) dan dilajutkan dengan uji Tukey. Hasil penelitian ini menunjukkan kokon yang diperoleh dari media pengokonan klaras memberikan hasil tertinggi pada bobot kokon utuh, bobot pupa, persentase pupa, bobot kulit kokon, panjang kokon, diameter kokon (anterior, medial, dan posterior), lingkar kokon (medial), serta persentase keberhasilan mengokon. Kokon ulat sutra yang diperoleh dari media pengokonan kayu memberikan hasil tertinggi pada parameter bobot serta persentase floss., Samia cynthia ricini (Lepidoptra: Saturniidae) is a non-mulberry silkworm that has been domesticated and its fibers have been widely used in the textile industry. In the cultivation of Eri silkworms, there are various types of mountages that can be used. Morphometric and cocooning percentage are indicators that need to be considered to determine the level of cocoon production. The purpose of this study is to analyze the effect of different mountages on the morphometrics of Eri silkworm cocoons and cocooning percentage. The treatments applied in this research were cocooning with dried banana leaves, rolled newspaper, and wooden mountage. This research used Completely Randomized Design (CRD) with three repetitions and a total of 270 fifth instar silkworms. The measured cocoons were taken randomly as many as 20 pieces from each mountage. Data were analyzed using Analysis of Variance (ANOVA) and continued with Tukey's test. The results showed that the cocoons collected from the dried babana leaves mountage produced the highest yields on cocoon weight, pupae weight, pupae percentage, cocoon shell weight, cocoon length, cocoon diameter (anterior, medial, and posterior), cocoon circumference (medial), and cocooning percentage. Cocoons collected from wooden mountage produced the highest weight and percentage of floss.
Keyword: cocoon, cocooning mountage, morphometric, Samia cynthia ricini

Judul: Strategi kebijakan pengelolaan kualitas air di Pelabuhan Muara Angke Jakarta Utara
Abstrak: Pelabuhan Muara Angke merupakan salah satu pelabuhan yang terletak di wilayah Teluk Jakarta. Letaknya yang sangat strategis dan didukung oleh sumberdaya alam sehingga menyebabkan tejadinya peningkatan aktivitas manusia, baik di darat maupun di lautan. Dari aktivitas ini menghasilkan limbah berupa limbah padat dan cair, sehingga menyebabkan terjadinya pencemaran terhadap kualitas air di sekitar kawasan Pelabuhan Muam Angke.
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