Judul: Analysis of Consumer Preferences in choosing a Coffee Shop in Bontang City
Abstrak: Kopi telah menjadi bagian dari gaya hidup dan digemari oleh masyarakat sehingga menjadi usaha yang menjanjikan. Semakin menjamurnya coffee shop di Kota Bontang menyebabkan persaingan bisnis sehingga terjadi fluktuasi penjualan. Untuk pemasaran yang efektif, pemilik coffee shop perlu memahami preferensi konsumen di Kota Bontang. Penelitian ini berfokus pada coffee shop di Kota Bontang Kalimantan Timur, dengan tujuan menentukan strategi berdasarkan perilaku konsumen. Metode penelitian menggunakan analisis konjoin dengan sampel 147 responden melalui purposive sampling. Lokasi menjadi atribut terpenting, diikuti oleh atmosfer, harga, fasilitas, pelayanan, dan produk. Konsumen cenderung memilih coffee shop yang dekat dengan tempat tinggal, harga Rp 10.000 – Rp 15.000, fasilitas Wifi, pelayanan cepat, dan tampilan menu yang menarik. Implikasi strategis dari penelitian ini dapat membantu pemilik coffee shop memahami preferensi konsumen dan meningkatkan pemasaran mereka di Kota Bontang., Coffee has become a part of lifestyle, embraced by the community and promising as a lucrative business. The proliferation of coffee shops in Kota Bontang has intensified business competition, leading to sales fluctuations. For effective marketing, coffee shop owners need to understand consumer preferences in Kota Bontang. This study focuses on coffee shops Bontang East Kalimantan, aiming to determine strategies based on consumer behavior. The research method employs conjoint analysis with a sample of 147 respondents selected through purposive sampling. Location emerges as the most crucial attribute, followed by atmosphere, price, facilities, service, and products. Consumers tend to choose coffee shops close to their residences, with prices ranging from Rp 10,000 to Rp 15,000, Wifi facilities, prompt service, and visually appealing menu displays. The strategic implications of this research can assist coffee shop owners in understanding consumer preferences and enhancing their marketing efforts in Kota Bontang.
Keyword: Consumer Preference, Coffee Shop, Conjoint Analysis

Judul: Consumer Behaviour on Making Decisions to Buy Coffee at Coffee shop Bogor
Abstrak: Bogor merupakan salah satu pasar coffee shop yang potensial. Hal ini dapat dilihat dari kebutuhan masyarakat menghabiskan waktu di coffee shop untuk mengisi waktu luang ataupun melakukan aktivitas seperti diskusi dan mengerjakan tugas. Hasil dari sensus penduduk Kota Bogor pada tahun 2021 mencatat mayoritas penduduk didominasi oleh Generasi Z dan Generasi Milenial. Tren coffee shop ini menjadi suatu bentuk aktualisasi diri terutama bagi generasi milenial dan generasi Z. Desain penelitian menggunakan cross sectional survey design. Penelitian difokuskan pada perikalu konsumen pada saat mengambil keputusan pembelian produk coffee shop. Penelitian akan dilakukan secara kuantitatif. Instrumen pada penelitian menggunakan kuesioner dalam bentuk Google Form yang disebarkan melalui link secara online di aplikasi media sosial seperti Instagram dan Whatsapp. Populasi pada penelitian ini adalah konsumen kopi di Kota Bogor. Menurut Hair, Anderson, Tatham & Black (2010) besarnya sampel bila terlalu besar akan menyulitkan untuk mendapat model yang cocok, dan disarankan ukuran sampel yang sesuai antara 100 - 200 responden agar dapat digunakan estimasi interpretasi dengan Structural Equation Model (SEM). Untuk itu jumlah sampel akan ditentukan berdasarkan hasil perhitungan sampel minimum. Hasil penelitian menunjukkan bahwa brand image tidak berpengaruh signifikan terhadap minat beli, brand image berpengaruh positif dan signifikan terhadap keputusan, atmosphere coffee shop berpengaruh positif dan signifikan terhadap minat beli, atmosphere coffee shop tidak berpengaruh signikan terhadap keputusan pembelian konsumen, service quality berpengaruh positif dan signifikan terhadap minat beli, service quality berpengaruh positif dan signifikan terhadap keputusan pembelian, minat beli berpengaruh positif dan signifikan terhadap keputusan pembelian, brand image tidak berpengaruh signifikan terhadap keputusan pembelian melalui minat beli sebagai variabel intervening, atmosphere coffee shop berpengaruh positif dan signifikan terhadap keputusan pembelian melalui minat beli sebagai variabel intervening, service quality tidak berpengaruh signifikan terhadap keputusan pembelian melalui minat beli sebagai variabel intervening. Saran yang dapat dianjurkan kepada pengusaha coffee shop adalah memertimbangkan strategi yang direkomendasikan sehingga dapat meningkatkan minat beli dan keputusan pembelian konsumen kopi di coffee shop. Objek penelitian ini adalah kopi di coffee shop Kota Bogor, oleh karena itu strategi yang disampaikan merupakan strategi yang dapat digunakan oleh pengusaha coffee shop di Kota Bogor., Bogor is one of the potential coffee shop markets. This can be seen from the need of people to spend time at coffee shops to fill their free time or carry out activities such as discussions and doing assignments. From Bogor City population census in 2021 record that the majority of the population is dominated by Generation Z and Millennial Generation. This coffee shop trend has become a form of self-actualization, especially for the millennial generation and generation Z. The research design used a cross-sectional survey design. The research is focused on consumer behavior when making purchasing decisions for coffee shop products. The research will be conducted quantitatively. The research instrument used a questionnaire in Google Form which was distributed via online links on social media applications such as Instagram and Whatsapp. The population in this study are coffee consumers in Bogor City. According to Hair, Anderson, Tatham & Black (2010) if the sample size is too large it will be difficult to obtain a suitable model, and it is
Keyword: Atmosphere Coffee Shop, Brand Image, Purchase Decision, Purchase Intention, Service Quality

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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