Judul: Analisis sebaran genetik hasil persilangan tanaman karet RRIM 600 dan PN 1546 menggunakan teknik RAPD
Abstrak: Siklus pemuliaan tanaman karet (Hevea brasiliensis Muell Arg.) yang membutuhkan waktu 25-30 tahun merupakan permasalahan dalam perakitan klon unggul karet. Deteksi dini klon unggulan karet dibutuhkan untuk menyelesaikan permasalahan ini, salah satunya dapat diatasi dengan memanfaatkan teknik random amplified polymorphic DNA (RAPD) yang merupakan salah satu jenis penanda molekuler DNA. Teknik RAPD dapat digunakan untuk melihat keragaman genetik melalui deteksi pola polimorfisme ruas nukleotida. Penelitian ini bertujuan untuk melihat keragaman genetik tanaman karet hasil persilangan jauh antara klon karet RRIM 600 dengan PN 1546 menggunakan teknik RAPD. Amplifikasi DNA dilakukan dengan menggunakan 16 primer kombinasi dan 40 primer RAPD. Pengolahan data dilakukan dengan menggunakan program NTSYS 2.02. Amplifikasi DNA dengan 16 primer kombinasi menghasilkan lima kandidat primer penanda molekuler, primer D spesifik untuk RRIM 600, primer C dan M spesifik untuk PN 1546, sedangkan primer N dan I spesifik untuk perpaduan kedua sifat tetua. Hasil amplifikasi DNA dengan 40 primer OPH-OPJ (1-20) menggunakan metode RAPD memperlihatkan keragaman genetik yang tinggi dengan nilai koefisien yang diperoleh sebesar 0.89-0.98. Terbentuk dua kelompok besar pada koefisien 0.89 untuk 27 tanaman karet. Dendogram hasil analisis juga memperlihatkan dua tetua terpisah pada percabangan yang berbeda yang menunjukkan bahwa kedua tetua memiliki hubungan kekerabatan yang jauh.
Keyword: RAPD technique, Eschercia coli, Hevea brasiliensis, Bogor Agricultural University, Institut Pertanian Bogor, IPB

Judul: Analisis Keragaman Genetik Tanaman Karet Hasil Persilangan antara RRIM 600 dan PN 1546 dengan Menggunakan Teknik RAPD
Abstrak: Low productivity of rubber product had been the main problem in public rubber plantation in Indonesia. New assembly of excellent genotipe rubber plant requires 25-30 years. The more appropriate and accurate method is needed to early determine the new rubber plant clone. This research aimed to shows genetic variation of rubber plants-crossed product between RRIM 600 and PN 1546 with RAPD technique. Two DNA samples from rubber plants parent and 25 DNA samples from rubber plants-crossed products were isolated and amplified by using 16 mTcCIR combination primers and 60 RAPD combination primers. Amplification by using 16 mTcCIR combination primers produced a specific selected marker candidates, while amplification by using 60 RAPD primers produced polymorphism ribbon pattern in agarose gel. Phylogenetic tree was created based on the ribbon to identify the group and family distances of rubber plants-crossed product. Result showed that combination primer ‟d‟ and ‟i‟can be developed into specific marker candidates for RRIM 600. Phylogenetic tree showed that 25 rubber plants-crossed product have 10 high family groups with coeficient 89%. The family group showed that 25 rubber plant-crossed product had high diversity.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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