Judul: sIlahkan mengisi gform pengajuan Bebas Pustaka pada link https://ipb.link/pta-perpusipb
Abstrak: Enzim tripsin merupakan enzim protease yang memiliki fungsi sebagai pemecah ikatan peptida protein. Sumber enzim tripsin komersial umumnya berasal dari pankreas hewan seperti babi dan sapi, namun isu mengenai kehalalan dan kesehatan menjadikan perlu adanya bahan alternatif lain salah satu contohnya yaitu usus ikan. Panjang usus ikan berbeda-beda yang dipengaruhi beberafa faktor seperti kebiasaan makan ikan. Berdasarkan kebiasaan makannya, ikan diklasifikasikan menjadi tiga jenis yaitu herbivora, karnivora, dan omnivora. Enzim tripsin dari usus ikan yang berbeda memiliki karakteristik yang berbeda pula. Penelitian ini bertujuan menentukan karakteristik dan stabilitas enzim tripsin dalam NaCl dari usus ikan berdasarkan perbedaan kebiasaan makan ikan. Ikan yang digunakan yaitu ikan baronang, cucut, dan sebelah. Uji yang dilakukan diantaranya yaitu menentukan suhu, pH optimum, pengaruh NaCl terhadap aktivitas enzim, kinetika, dan bobot molekul pada enzim tripsin. Aktivitas enzim tripsin dari ketiga jenis ikan optimum pada suhu 50ºC, pH 8, stabil terhadap larutan NaCl, dan memiliki bobot berkisar 21,9-27,2. Kebiasaan makan ikan yang berbeda tidak mempengaruhi karakteristik enzim tripsin.
Keyword: fish intestine, stability of NaCl, trypsin enzyme

Judul: Characteristics and Stability of Trypsin Enzyme in NaCl Isolated from Intestine, Liver, and Spleen of Tuna
Abstrak: Kebutuhan enzim di Indonesia sangat tinggi dan masih diimpor dari negara lain. Tripsin komersial biasanya diekstraksi dari pankreas babi dan sapi, sehingga diperlukan sumber alternatif lainnya yang bersumber dari ikan, yaitu usus, hati, dan limpa ikan tuna. Penelitian ini bertujuan menentukan karakteristik dan stabilitas enzim tripsin dalam NaCl, serta hubungan usus, hati, dan limpa ikan tuna terhadap aktivitas tripsin menggunakan Principal Component Analysis (PCA). Aktivitas tripsin berada pada kondisi optimum pada suhu 60 ̊C dan pH 8 dengan nilai aktivitas spesifik pada usus sebesar 0,948±0,114 U/mg; hati sebesar 0,610±0,029 U/mg; dan limpa sebesar 0,605±0,159 U/mg. Kecepatan reaksi maksimum (Vmaks) menunjukkan nilai usus yang paling besar sebesar 0,248 mmol/s, diikuti oleh hati 0,138 mmol/s, dan limpa 0,096 mmol/s, sedangkan nilai konstanta (Km) yang diperoleh usus sebesar 2,342 mM; hati sebesar 2,268 mM, dan limpa sebesar 1,276 mM. Bobot molekul pada tripsin berkisar antara 20-30 kDa. Konsentrasi NaCl yang tinggi akan menyebabkan aktivitas semakin menurun. Bagian usus, hati, dan limpa memiliki korelasi positif., The need for enzymes in Indonesia is very high and they are still imported from other countries. Commercial trypsin is usually extracted from the pancreas of pigs and cattle, so that other alternative sources are needed from fish, namely the intestines, liver and spleen of tuna. The relationship between intestine, liver and spleen of tuna trypsin activity using Principal Component Analysys (PCA) was used to determine characterisics and stability of the trypsin enzyme in NaCl as the study aims. Trypsin activity was at its optimum at a temperature of 60 ̊C and pH 8 with a specific activity value in the intestine of 0,948 ± 0,114 U/mg; liver of 0,610 ± 0,029 U/mg; and spleen of 0,605 ± 0,159 U/mg. The maximum reaction speed (Vmax) showed the largest intestinal value of 0,248 mmol/s, followed by the liver 0,138 mmol/s, and the spleen 0,096 mmol/s, while the constant value (Km) obtained by the intestine was 2,342 mM; liver of 2,268 mM, and spleen of 1,276 mM. The molecular weight of trypsin ranges from 20-30 kDa. The high concentration of NaCl decreased the activity. Sections of intestine, liver, and spleen had a positive correlation.
Keyword: karakteristik enzim tripsin, stabilitas NaCl, jeroan tuna

Judul: Prediction Model Development Madden Julian Oscillation (MJO) based on the results of data analysis Real Time Multivariate MJO (RMM1 and RMM2).
Abstrak: Background of this research is the importance of study on the Madden Julian Oscillation, the dominant oscillation in the equator area. MJO cycle showed by cloud cluster growing in the Indian Ocean then moved to the east and form a cycle with a range of 40-50 days and the coverage area from 10N-10S. Method that used to predict RMM is Box-Jenkins based on ARIMA (Autoregressive Integrated Moving Average) statistical analysis. The data used RMM daily data period 1 March 1979 –1 March 2009 (30 years). RMM1 and RMM2 is an index for monitoring MJO. This is based on two empirical orthogonal functions (EOFs) from the combined average zonal 850hPa wind, 200hPa zonal wind, and satellite-observed Outgoing Longwave Radiation (OLR) data. The results in form of the Power Spectral Density (PSD) graph Real Time Multivariate MJO (RMM) and long wave radiation (OLR = Outgoing Longwave Radiation) at the position 100°BT, 120°BT, and 140°BT that show the wave pattern (spectrum pattern) and clearly shows the oscillation periods. There is a close relation between RMM1 with OLR at the position 100oBT that characterized the PSD value about 45 day. Through Box-Jenkins method, the prediction model that close to time series data of RMM1 and RMM2 is ARIMA (2,1,2), that mean the forecasts of RMM data for the future depending on one time previously and the error one time before. Prediction model for RMM1 is Zt = 1.681 Z t-1 – 0.722 Z t-2 + 0.041 Zt-3 - 0.02 at-1 - 0.05 at-2.. Prediction model for RMM2 is Zt = 1.714 Zt-1 – 0.764 Zt-2 - 2 + 0.05 Zt-3 - 0.109 at-1 - 0.05 at- 2.. The flood case in Jakarta January-February 1996 and 2002 are one of real evidence that made the MJO prediction important. MJO with active phase dominant cover almost the entire Indonesia west area at that moment. Keywords: MJO, RMM1 and RMM2, OLR, Power Spectral Density, ARIMA, Rainfall
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