Judul: Analisis Perhitungan Sumur Resapan dan Kebutuhan Air di Persemaian Permanen Kampus IPB Dramaga
Abstrak: Program green campus 2020 Institut Pertanian Bogor perlu mempertimbangkan pengelolaan air permukaan salah satunya dengan pembangunan sumur resapan. Penelitian ini bertujuan untuk menghitung jumlah sumur resapan pada lokasi penelitian dan jumlah kebutuhan air di persemaian permanen IPB. Pendugaan limpasan menggunakan metode SCS-CN didapatkan nilai sebesar 46 496 m³ dengan luas lahan terbangun 19 400 m². Koefisien permeabilitas tanah diperoleh 0.508 m/jam di Asrama PPKU, 0.234 m/jam di persemaian permanen, 0.198 m/jam di Mesjid Al Hurriyah dan 0.378 di Wisma Landhuis. Jumlah sumur yang harus dibangun pada lokasi penelitian adalah 36 unit di asrama PPKU, 2 unit di persemaian, 19 unit di Al Hurriyyah, 14 unit Wisma Landhuis, 2 unit di kantin RC dan 8 unit di SMA Kornita. Jumlah air yang diresapkan pada lokasi penelitian adalah 10 m³ di asrama PPKU, 18 m³ di persemaian, 21 m³ di Al Hurriyyah dan 14 m³ di Wisma Landhuis. Kebutuhan air persemaian permanen Dramaga Bogor didapatkan sebesar 95.58 m³/hari atau 21 413.46 m³ selama 260 hari tidak terjadi hujan. Jumlah tersebut setara dengan 6 buah sumur resapan yang harus dibangun untuk memenuhi kebutuhan air di persemaian supaya sumur di persemaian permanen tidak kering.
Keyword: sumur resapan, kebutuhan air, persemaian permanen, IPB Dramaga

Judul: Rancangan Sistem Penampungan Air Hujan Pada Bangunan Hijau Di Kampus IPB Dramaga Bogor
Abstrak: Indonesia merupakan negara beriklim tropis dengan curah hujan rata-rata yaitu antara 2.000-4.000 mm/tahun (KLH 2009). Potensi air hujan yang tinggi belum termanfaatkan secara optimal. Siklus hidrologi memegang peranan penting dalam menjaga ketersediaan air di permukaan bumi. Penelitian ini bertujuan menyusun desain penampungan air hujan, menghitung kebutuhan air domestik, dan menghitung persentase kebutuhan air domestik yang disuplai dari air hujan pada gedung kuliah baru IPB. Penelitian ini menggunakan data curah hujan harian 10 tahun terakhir, yaitu dari tahun 2010-2019 dengan acuan SNI 03-7065-2005 dan SNI 8153:2015. Hasil penelitian ini berupa Ground Water Tank (GWT) berukuran 6 x 4,5 x 4,5 x 0,3 m dengan kapasitas 121,92 m3 yang dapat memenuhi kebutuhan air gedung sebesar 78,90 %. Penelitian ini menggunakan sistem atap sebagai catchment area dari ground water tank. Debit andalan yang didapat berdasarkan luas area atap sebesar 53,65 m3. Penilaian Greenship kategori aspek konservasi air memperoleh poin sebesar 17 poin dari total 21 poin yang tersedia dengan dua tolak ukur prasyarat. Rancangan Anggaran Biaya (RAB) pembuatan ground water tank di gedung kuliah baru IPB sebesar Rp 347.905.662., Indonesia is a tropical country with an average rainfall of 2,000-4,000 mm/year (KLH 2009). The high potential of rainwater has not been utilized optimally. The hydrological cycle plays an important role in maintaining the availability of water on the earth's surface. This study aims to develop a rainwater storage design, calculate domestic water needs, and calculate the percentage of domestic water needs supplied from rainwater in the new IPB lecture building. This study uses daily rainfall data for the last 10 years, namely from 2010-2019 with reference to SNI 03-7065-2005 and SNI 8153:2015. The results of this study are Ground Water Tank (GWT) measuring 6 x 4,5 x 4,5 x 0.3 m with a capacity of 121,92 m3 which can meet the building's water needs of 82,90%. This study uses a roof system as a catchment area of the ground water tank. The mainstay discharge obtained based on the roof area is 53,65 m3. The Greenship assessment in the water conservation aspect category received 17 points out of a total of 21 points available with two prerequisite benchmarks. The Draft Budget (RAB) for the construction of a ground water tank in the new lecture building of IPB amounted to Rp 347.905.662.
Keyword: clean water needs, water conservation, rainwater storage

Judul: Mitochondrial DNA Gene Organization and Phylogenetic Relationship of Naja siamensis
Abstrak: Naja siamensis is one of Asiatic cobra in the Family of Elapidae, known as a cryptic snake. It has many morphological variations that lead to misidentification. Misidentification can cause the wrong concept in developing antivenom types due to high snakebite cases occur in Thailand. Thus, an accurate species identification using a molecular approach is crucially required. Mitochondrial DNA has been used widely in snake molecular studies. These studies were aimed to determine the arrangement of the N. siamensis partial mitochondrial genes using reptile-oriented primers and to reveal the phylogenetic relationship among other species of Asian cobra. DNA extraction was carried out based on the standard DNA salting-out protocol and was conducted by bioinformatics analysis using BLASTn and MEGA 6 software. Gene annotation was performed against N. kaouthia complete genome. A total of 3121 bp of N. siamensis mtDNA genes were sequenced consisting of partial of six regions: 16S rRNA, ND1, tRNA-Ile, CRII region, tRNA-Leu, tRNA-Gln, and COI genes. The mtDNA gene organization of N. siamensis was predicted to has a similar pattern with the common Elapidae mtDNA gene organization. This study showed that there is a monophyletic form in Naja genus and N. kaouthia is the closest species with N. siamensis based on partial 16S rRNA and ND1 gene.
Keyword: 16S rRNA, Elapidae, ND1, phylogenetic relationship