Judul: Analisis Integrasi dan Ekspresi Gen Ketahanan terhadap Geminivirus (AV1) pada Tembakau Transgenik
Abstrak: Geminivirus infection in several vegetable crops such as tomato and chilli has caused leaf curl and yellowing disease. This disease has significantly impact on yield losses of vegetable production in Indonesia. Genetic engineering technology gives an oppurtunity to develop transgenic plant resistant to Geminivirus. Geminivirus AV1 gene expressing coat protein can be used to develop virus resistant plant. The objective of this research was to identify the integration and expression of AV1 gene in tobacco transgenic plant for resistance to Geminivirus. Analysis of AV1 gene integration was carried out by using polymerase chain reaction (PCR) technique and AV1 gene specific primer as well as tobacco genomic DNA as template. Meanwhile, expression analysis of AV1 gene in transcriptional level was conducted by isolating total RNA, cDNA sintesis using reverse transcriptase-PCR (RT-PCR) and amplification AV1 gene product with the cDNAs as a template. Result of this research showed that out of 60 tobacco plants generation T1 there are 36 plants containing AV1 gene and 18 plants of them showed resistant to Geminivirus. Analysis of AV1 gene expression on 6 selected plants showed that 4 plants expressed AV1 gene that indicated by PCR product from cDNA template.
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Judul: Deteksi Geminivirus yang menginfeksi tanaman terung (Solanum melongena L.) dengan teknik Polymerase Chain Reaction.
Abstrak: Diseases caused by whitefly-transmitted Geminiviruses (WTGs) have become serious constraints for crops throughout the world, including Indonesia. WTGs has been reported to cause significant yield loss especially in chili pepper and tomato in Java. This research aims to detect and identify Geminivirus infecting eggplant using polymerase chain reaction (PCR) and nucleotide sequencing. The methods involved DNA extraction using CTAB, DNA amplification and nucleotide sequence analysis by Bioedit V 7.0.5 and ClustalW. Three pair of universal primers, i.e. pAL1v1978/pAR1c715, SPG1/SPG2, pAV494/pAC1048, was used for DNA amplification. Infection of Geminivirus was evidenced from leaves collected from West Java (Bandung) and Central Java (Pati, Rembang, Bantul). Universal primer SPG1/SPG2 was able to amplify DNA fragments of ≈912 bp from all leaf samples. Nucleotide analyses showed that Geminivirus infecting eggplant from West Java and Central Java has a close relationship (98.8%) with Tomato yellow leaf curl Kanchanaburi virus (TYLCVKaV) from Thailand.
Keyword: universal primers., sequence analysis, DNA amplification

Judul: Measurement of consciousness in dreaming in adults  using lucid scale
Abstrak: Tidur adalah keadaan saat otak tidak mengolah informasi dari neuron sensorik dan tidak memberi perintah ke neuron motorik. Ketika tidur seseorang bermimpi di mana kesadaran berubah karena otak membangun dunia maya dengan gambaran yang nyata dan tidak dapat kita identifikasi sebagai halusinogen. Lucid dream atau mimpi sadar berkaitan dengan peningkatan konektivitas fungsional antara korteks prefrontal dan area asosiasi temporoparietal. Kedua wilayah ini biasanya dinonaktifkan selama tidur dan pengaktifannya menyebabkan lucid dream di mana orang sadar bahwa pengalaman saat ini adalah milik mimpi. Kita dapat mengukur kesadaran dalam bermimpi dengan menggunakan lucid scale. Namun demikian, Voss et al. (2013) tidak menentukan seberapa skala yang menjadi batas apakah seseorang itu lucid dreamer atau bukan. Untuk menjawab pertanyaan tersebut, penelitian ini bertujuan untuk mengetahui apakah seorang lucid dreamer itu mempunyai skala lucidity yang lebih tinggi daripada orang yang non-lucid dreamer. Penelitian ini menemukan bahwa pada responden dewasa metode pengukuran lucid scale berlaku sebagai indikator lucid dreamer untuk responden laki-laki namun metode ini tidak berlaku untuk responden perempuan. Skala lucidity berkaitan dengan kemampuan penalaran pada laki-laki, tetapi tidak demikian halnya pada perempuan.
Keyword: lucid dream, sleep, lucid scale, reasoning