Judul: Evaluasi Perseptual Kualitas Estetika dan Ekologi Objek Wisata Kebun Raya Cibodas
Abstrak: The usage of Cibodas Botanical Garden as a recreation area, as well as a collection function with its natural beauty, can change the aesthetics and ecological quality of this garden. An aesthetics and ecological quality assessement with Scenic Beauty Estimation (SBE) and Semantic Differential methode were applied. Eleven area spreading over three zone (service area, collection area, and recreation area) had been assessed their quality and characteristic into three group i.e. good, fair, and bad. Some of them had been changed far enough from their nature. Lawn (recreation area) showed good aesthetics quality and Rhododendron Garden (collection area) have bad aesthetics quality. Result of the evaluation on ecological quality showed that almost all of vantage point in the garden showed good ecological quality except some areas such as Rhododendron Garden and Ciismun Waterfall that have bad quality. Some recommendation how to increase landscape intregrity of the Garden were elaborate on the basis of these research result.
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Judul: Persepsi pengunjung terhadap fungsi kebun raya Cibodas sebagai sarana pendidikan dan lingkungan dan preferensi terhadap bentuk wisata edukatif
Abstrak: Pariwisata merupakan bagian yang tidak terpisahkan dari kehidupan manusia terutama menyangkut kegiatan sosial dan ekonomi. Pariwisata memiliki peranan penting dalam sistem perekonomian Indonesia. Di sisi lain, Indonesia memiliki kekayaan alam yang menjadikan Indonesia berpotensi untuk menjadi tujuan wisata dunia. Dengan bentuk pengembangan produk wisata yang beragam. Salah satu bentuk pariwisata alternative adalah ecotourisme atau ekowisata. Salah satu bentuk dari ekowisata adalah Kebun Raya Cibodas. Kebun Raya Cibodas (KRC) merupakan tempat wisata di Kabupaten Cianjur yang paling banyak dikunjungi masyarakat. Rekreasi di alam terbuka juga dapat menjadi wahana bagi pembinaan masyarakat dalam mencintai alam, mengenal kegiatan pelestarian dan pemanfaatan sumberdaya alam, serta mengenal proses-proses ekologi yang mendukung sistem kehidupan. Hal ini akan terlaksana jika masyarakat yang berekreasi mendapat bimbingan dan pengarahan, informasi dan fasilitas yang tepat. Fasilitas dan bentuk rekreasi harus disediakan sesuai dengan preferensi masyarakat serta mampu menginformasikan pengetahuan secara efektif dan efisien. Sehingga pemenuhan kebutuhan akan rekreasi sejalan dengan peningkatan kesadaran masyarakat akan lingkungan dan pelestarian sumber daya alam. Berdasarkan perumusan masalah diatas maka tujuan dari penelitian ini adalah:1) Mengukur persepsi pengunjung terhadap fungsi Kebun Raya sebagai sarana pendidikan lingkungan. 2) Menganalisis Faktor-faktor apa saja yang berhubungan dengan persepsi pengunjung terhadap fungsi Kebun Raya sebagai sarana pendidikan lingkungan. 3) Mengetahui preferensi pengunjung akan bentuk wisata yang edukatif...
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Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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