Judul: Pengembangan Model Artifical Neural Network untuk Pendugaan Suhu Udara Dalam Greenhouse dengan Berbagai Kemiringan Atap pada Single-span Greenhouse
Abstrak: Greenhouse adalah bangunan tembus cahaya sehingga memungkinkan cahaya yang dibutuhkan tanaman masuk kedalamnya, dan menghindarkan tanaman dari pengaruh yang kurang menguntungkan. Greenhouse yang dibutuhkan di Indonesia adalah greenhouse yang berfungsi sebagai pelindung tanaman dari terpaan hujan dan mengurangi intensitas serangan hama dan cahaya matahari yang berlebihan.
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Judul: Artificial Neural Networks Modelling for  Estimating Air Temperature in a Greenhouse under Evaporative Cooling
Abstrak: Suhu udara di dalam greenhouse di kawasan tropika basah pada siang hari ketika cuaca cerah cenderung terlalu tinggi untuk pertumbuhan tanaman. Salah satu upaya untuk menurunkan suhu udara di dalam greenhouse tersebut adalah dengan menggunakan sistem evaporative cooling. Namun, sistem tersebut belum tentu mampu menurunkan suhu mencapai syarat lingkungan tumbuh untuk tanaman yang berasal dari dataran tinggi. Sistem evaporative cooling dengan penambahan suatu water chiller dapat menurunkan suhu air sehingga suhu udara di dalam greenhouse mendekati suhu optimum untuk tanaman dataran tinggi. Dalam hubungan ini, suatu model untuk menduga suhu udara di dalam greenhouse akan sangat berguna dalam perancangan maupun pengendalian lingkungan di dalam greenhouse. Penelitian ini bertujuan mengembangkan model Artificial Neural Network (ANN) untuk menduga suhu udara di dalam greenhouse. Pengembangan model ANN dilakukan dengan menentukan nilai terbaik untuk beberapa parameter model. Parameter model tersebut antara lain adalah random state, initial learning rate, momentum, dan hidden layer sizes. Model ANN struktur 8-7-1 dengan algoritma Backpropagation yang dikembangkan memiliki kinerja yang sangat baik dalam menduga suhu udara di dalam greenhouse di titik-titik representasi dengan nilai R2 di atas 0,99 dan RMSE di bawah 0,15 oC
Keyword: greenhouse, artificial neural network, evaporative cooling, water chiller

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.