Judul: Keterkaitann konsumsi pangan dan status gizi dengan usia menarche anak perempuan usia sekolah dasar di Bogor
Abstrak: Tujuan penelitian ini adalah menganalisis hubungan konsumsi makanan dan status gizi dengan usia menarche pada anak sekolah dasar di Kota Bogor. Penelitian menggunakan desain cross sectional study, dilakukan pada bulan September sampai November 2011 di enam sekolah dasar di Bogor. Sampel dipilih secara purposif sehingga diperoleh 58 siswa. Data dianalisis menggunakan uji rank spearman. Hasil penelitian menunjukkan rata-rata usia menarche adalah 10,74± 0,71. Lebih dari separuh (62,1%) siswa mengalami menarche pada usia ≥ 10 tahun. Usia menarche berhubungan negatif signifikan dengan konsumsi zat besi tetapi tidak berhubungan dengan status gizi, usia ibu menarche, jumlah anggota keluarga, pengetahuan gizi anak, latar belakang pendidikan ayah, dan pendapatan rumah tangga. Penelitian ini menyarankan agar orang tua lebih memberikan informasi dan dukungan mengenai aspek-aspek yang berkaitan dengan pubertas.
Keyword: Food consumption, Nutritional status, Menarche, Elementary school, Puberty

Judul: Studi konsumsi pangan hewani dan waktu pubertas pada siswi sekolah dasar di Bogor
Abstrak: Currently the consumption of animal food of Indonesia's population is still low, which only reached 6.6 kg/capita/year (Hanani 2009). Consumption of livestock products of Indonesians in 2008 (BPS 2010b) was 4.8 kg/capita/year for meat, 17.42 kg/capita/year for eggs, and 0.28 kg/capita/year for milk. This study aims to determine the frequency of consumption of animal food among female elementary school students in the City of Bogor and its association to menarche time of puberty.The design used was cross-sectional study. The total sample consisted of 38 female students who were menstruating and 38 female students who have not been menstruating. Most of the samples (84.21%) experienced first menstruation at age 9-11 years.The most common animal food consumption of menstruating students was milk with an average of 9.8 ± 7.0 times/week. Chicken and milk were often consumed by students of this group with a percentage of 97.4%. Whereas the most common animal food consumption of non-menstruating students was also milk with an average frequency of consumption 13.3 ± 8.4 times/week. Eggs were most often consumed by students of this group with the percentage of 100%. There was a significant association between the frequency of milk consumption with the amount of pocket money of students who are menstruating (p=0.037). Demonstrated a significant association between knowledge of nutrition with a frequency of egg consumption (p=0.010) in students who have periods while students who have not menstruation showed no association between these variables. There was an apparent association between the FFQ sausage meat with the age at first menstruation (p=0.022). There was no significant association between age at first menstruation with nutritional status (height for age, p=0.788 and BMI for age, p=0.060) and consumption of nutrients (energy, p=0.993 and protein, p=0.889) and the rate of nutrient adequacy (energy, p=0.905 and protein, p=0.847). In the group of students who have menstrual periods there was no significant association between nutritional status with sufficient levels of nutrition (p>0.05).
Keyword: 

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
Keyword: