Judul: Pengaruh Modal Sosial dalam Keluarga dan Pengasuhan Penerimaan-Penolakan terhadap Karakter Kesadaran dan Kontrol Diri Anak
Abstrak: The decreasing values of kindness among school-age children could be caused by their low conscience and self-control. The aims of this study was to analyze social capital within family, parental acceptance-rejection, and its influence on the character of conscience and self control of the school-age children. Samples frames of this research were the family with the first child is in school-age (10-12 years) who lived in the Situ Gede Village. This research involved 50 samples was randomly taken and interviewed by the questionnaire. The level of social capital within family was measured by instrument that was adopted from Coleman and Hoffer (1987), parenting style measured by Parental Accetance-Rejection Questionnaire (PARQ), the characters was measured by adopted questionnaire from Borba (2001). The results showed that longer father’s formal education is significant correlated with higher character of conscience and self control of the child. Meanwhile, longer mother’s formal education is significant correlated with higher child’s self control and lower father’s violent behavior. Longer age differences between paternal age is significant correlated with lower parental’s neglect behaviour. Characters of conscience and self control correlated with the level of parental closeness, father’s affective behavior, and parental rejection. Regression analysis showed that the characters of conscience and self control of the child are influenced by father's formal education, social capital within family, and parental rejection.
Keyword: 

Judul: Parents Emotional Coach, Adolescent’ Self Esteem and The Influence on The Character of Politeness Character of High School Students in The Cirebon Coastal Area
Abstrak: Kesadaran orang tua sebagai pelopor karakter bagi anak masih cukup rendah. Keluarga (orang tua) merupakan tempat utama dan orang pertama yang dapat membantu anak dalam memberikan pelatihan emosi kepada anak agar memiliki harga diri (self- esteem) yang baik dan menjadi pribadi berkarakter baik dalam aspek kesantunan. Penelitian ini bertujuan untuk menganalisis hubungan di antara variabel orang tua pelatih emosi, harga diri, dan karakter santun siswa SMA di daerah pesisir Cirebon. Penelitian ini merupakan penelitian kuantitatif dengan desain penelitian cross sectional study dengan melibatkan 60 siswa di salah satu SMA Negeri di Cirebon Utara. Hasil penelitian menunjukkan bahwa orangtua di daerah pesisir ini umumnya menerapkan gaya pengasuhan orang tua pelatih sudah cukup baik, harga diri siswa SMA cenderung rendah, sedangkan karakter santun terkategori rendah. Uji hubungan menunjukkan bahwa terdapat hubungan negatif signifikan antara pendidikan ayah dengan harga diri remaja dan terdapat hubungan positif signifikan antara harga diri remaja dengan karakter santun. Hasil uji pengaruh menunjukkan bahwa harga diri berpengaruh positif (β = 0.811) terhadap karakter santun., The awareness of parents as character pioneers for children is still quite low. The family (parents) is the main place and the first person who can help children in providing emotional training to children so that they have good self-esteem and become individuals with good character in the aspect of politeness. This study aims to analyze the relationship between the variables of emotional coach parents, self-esteem, and polite character of high school students in the coastal area of Cirebon. This research is a quantitative research with a cross sectional study design involving 60 students in one of the public high schools in North Cirebon. The results showed that parents in this coastal area generally applied the parenting style of coaches which was quite good, high school students' self-esteem tended to be low, while polite character was categorized as low. The relationship test showed that there was a significant negative relationship between father's education and adolescent self- esteem and there was a significant positive relationship between adolescent self-esteem and polite character. The results of the influence test show that self-esteem has a positive effect (β = 0.811) on polite character.
Keyword: Orang tua pelatih emosi, harga diri, karakter santun, Parents emotional coach, self-esteem, politeness of character

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable