Judul: Valuasi Ekonomi Pemanfaatan Ekosistem Hutan Mangrove di Dukuh Pandansari, Kecamatan Brebes, Kabupaten Brebes, Provinsi Jawa Tengah
Abstrak: Dukuh Pandansari, Kecamatan Brebes, Kabupaten Brebes, Jawa Tengah merupakan salahsatu wilayah yang memiliki potensi ekosistem hutan mangrove yang cukup terjaga. Berdasarkan data BPS Kabupaten Brebes, kawasan ekosistem mangrove ini memiliki luas 333,29 ha. Berdasarkan hasil penghitungan pada penelitian ini kawasan ekosistem hitan mangrove di Dukuh Pandansasri memiliki nilai ekonomi total sebesar Rp4.079.254.293,00/tahun atau sebesar Rp12.239.354,00/ha/tahun. Nilai ekonomi terbesar diperoleh dari nilai manfaat langsung untuk penangkapan udang, kepiting dan rajungan serta ikan, yaitu sebesar Rp2.953.969.714,00 atau sebesar 72,41%. Kedua nilai manfaat tidak langsung sebesar Rp977.748.762,00 atau 23,97%, kemudian nilai manfaat pilihan sebesar Rp79.344.683,00 atau 1,95% dan yang terakhir yaitu nilai manfaat keberadaan sebesar Rp68.191.134,00 atau 1,67%.
Keyword: mangrove, economic valuation, Pandansari Village, Brebes District

Judul: Penilaian Jasa Penyediaan dan Pengaturan Ekosistem  Mangrove di Pandansari Brebes Jawa Tengah
Abstrak: Ekosistem mangrove Pandansari, Brebes, Jawa Tengah merupakan salah satu sumberdaya alam pesisir yang potensial untuk dimanfaatkan. Ancaman alih fungsi lahan menunjukkan kurangnya kesadaran masyarakat sekitar akan pentingnya manfaat ekosistem mangrove. Penelitian ini bertujuan mengestimasi total ekonomi nilai guna langsung dari jasa provisoning dan tidak langsung dari jasa regulating ekosistem mangrove Pandansari. Metode pengambilan sampel yang diterapkan dalam penelitian ini adalah purposive sampling dengan pendekatan pengumpulan data melalui wawancara. Analisis data yang digunakan, yakni melalui pendekatan permintaan melalui metode EOP (effect on production) dan pendekatan contingen valuation method (CVM) berupa willingness to pay (WTP). Nilai ekonomi dari jasa provisioning ekosistem mangrove sebagai penyedia perikanan tangkap, yakni sebesar Rp124.401.446/ha/tahun, sedangkan nilai ekonomi dari jasa regulating ekosistem mangrove sebagai pengendali banjir, yakni sebesar Rp1.625.340.000/tahun., The Pandansari mangrove ecosystem in Brebes, Central Java, is one of the coastal natural resources with potential for utilization. The threat of land-use change indicates a lack of awareness among the local community about the importance of the mangrove ecosystem's benefits. This study aims to estimate the total economic value of direct use from provisioning services and indirect use from regulating services of the Pandansari mangrove ecosystem. The sampling method applied in this research is purposive sampling with a data collection approach through interviews. The data analysis used, is the demand approach through the Effect on Production (EOP) method and the Contingent Valuation Method (CVM) in the form of willingness to pay (WTP). The economic value of the provisioning services of the mangrove ecosystem as a provider of capture fisheries is Rp124,401,446/ha/year; while the economic value of the regulating services of the mangrove ecosystem as a flood controller is Rp1,625,340,000/year.
Keyword: regulating services, flood control, provisioning  services, Capture fisheries, economic valuation

Judul: Excretory/secretory antigen of ascaridia galli L3 stage to trigger yolk immunoglobulin in laying hens
Abstrak: Ascaridiosis caused by the nematode Ascaridia galli. A study was carried out to characterize excretory/secretory antigen of A. galli from larval stage to trigger anti-ascaridiosis yolk immunoglobulin in laying hens. A. galli L3 were recovered from intestines of 100 heads chickens 7 days after oesophagus inoculation with 6000 L2. L3 recovered in this manner were cultured (5 – 10 ml-1) in flasks containing rosswell park memorial institute (RPMI) 1640 media, pH 6.8, without phenol red. Cultures were incubated at 370C in 5% CO2 and culture fluid was collected after 3 days in culture. To prepare excretory/secretory antigen, culture medium were concentrated with vivaspin 30.000 MWCO, and antigen protein concentration were counted as described in Bradford method. The molecular weight of excretory/secretory antigen was determined with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). Active immunizations were applied intra muscularly of chickens with an initial dose of 80 μg. The immunizations were repeated three times with dose of each 60 μg with an interval of one week. The first immunizations were excretory/secretory antigen mixed with fruend adjuvant complete and subsequently mixed with freund adjuvant incomplete. The chicken’s eggs were collected from 49 day after immunizations. Anti-ascaridiosis yolk immunoglobulin (IgY) was extracted from egg yolks by means of ammonium sulphate and purified using fast performance liquid chromatography (FPLC). The purity of anti-ascaridiosis IgY was determined by Bradford method (λ = 280 nm). Antibody was detected by agar gel precipitation test (AGPT) and enzyme linked immunosorbant assay (ELISA). Antigen were detected with immunohistochemistry. Chickens were challanged with dose of 1000 L2 A. galli. Passive immunizations were applied ten times with dose of each 0,875 mg IgY with an interval of one day intra orally. IgY anti-ascaridiosis was tested against A. galli survival in vitro.
Keyword: Unggas, Isolasi dan karakterisasi antigen, Purifikasi imunoglobulin yolk, Pengukuran antibodi ayam petelur, Metode Imunohistokimia, Ascaridiosis