Judul: Struktur dan Komposisi Vegetasi Pascakebakaran Hutan di Hutan Pendidikan Gunung Walat, Jawa Barat
Abstrak: Kebakaran hutan dapat menyebabkan perubahan vegetasi sesuai tergantung kepada intensitas kebakaran yang terjadi, sehingga akan membentuk pola mosaik yang terdiri atas berbagai fase suksesi. Kebakaran hutan selain terjadi pada hutan tanaman dan alam juga dapat terjadi pada hutan pendidikan seperti kebakaran yang terjadi pada Hutan Pendidikan Gunung Walat (HPGW). Kebakaran hutan yang terjadi di HPGW dimulai sejak tahun 2002 dimana kebakaran yang terjadi menimbulkan dampak yang cukup besar baik secara ekologis, ekonomis, dan sosial. Banyaknya pohon yang terbakar menyebabkan terjadinya perubahan pada kondisi hutan di HPGW. Tujuan penelitian ini adalah untuk menganalisa dampak kebakaran hutan terhadap vegetasi terutama dalam hal perubahaan struktur dan komposisi tegakan pada areal bekas terbakar. Jenis tumbuhan bawah mendominasi pada tingkat tumbuhan bawah yaitu jenis Rolandra fruticosa, Centotheca lappacea, Clibadium surinamnsis, dan Oldenlandia auricularia sedangkan pada plot pengamatan tidak terbakar didominasi oleh Salaginella plana, Oldenlandia auricularia, dan Clidemia hirta. Pada tingkat semai jenis yang mendominasi ialah jenis Schima wallichi. Komposisi jenis pada tingkat pertumbuhan semai, pancang, tiang, pohon, dan tumbuhan bawah pada vegetasi terbakar lebih beranekaragam dan merada dibandingkan dengan vegetasi tidak terbakar. Indeks kesamaan komunitas pada tingkat pertumbuhan pohon menunjukkan kesamaan pada kedua komunitas tersebut, sedangkan pada tingkat semai, pancang, tiang dan tumbuhan bawah menunjukkan kedua komunitas tersebut dikatakan berbeda.
Keyword: Dampak kebakaran hutan, Hutan Pendidikan Gunung Walat, Kebakaran huta

Judul: Komposisi Vegetasi pada Lahan Bekas Terbakar di Hutan Pendidikan Gunung Walat
Abstrak: The resiliency of the ex-burnt forest area will following the forest succession stages and resulted to secondary forest. This study was conducted to identifies: (1) type and cause of forest fire on September 2012 at Hutan Pendidikan Gunung Walat (HPGW), (2) differences of the vegetation composition of stands, (3) undergrowth succession, as well as (4) differences of soil chemical properties in the ex-burnt and unburnt stands. Fire in HPGW was mainly due to the careless use of fire by local communities when they used fire to burn thatch on their land. Highest index of similarity is found on tree stage at all plots, while the most significant differences found on undergrowth stage. This may related to the vulnerability of the undergrowth to evey changes that occur, one of which is the change on soil chemical properties. Generally, the soil chemical properties at 11th month after fire is better than 6th month after fire.
Keyword: undergrowth, succession, soil chemical properties, forest fire, composition of vegetation

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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