Judul: The Behavior of Jonggol Sheep for 5 Hours After Long-Distance Transportation with Different Densities
Abstrak: Stres pada ternak akibat transportasi dapat dilihat dari perilaku ternak setelah proses transportasi. Penelitian ini bertujuan untuk mengevaluasi perbedaan tingkah laku domba Jonggol pada 5 jam pasca transportasi jarak jauh dengan kepadatan kendaraan yang berbeda. Lokasi penelitian ini berada di UP3J (Unit Pendidikan dan Penelitian Peternakan Jonggol) IPB dan Laboratorium Lapangan Ruminansia Kecil Blok B, Fakultas Peternakan IPB. Penelitian ini dilaksanakan selama 7 hari pada September 2022. Penelitian ini menggunakan 27 ekor domba Jonggol yang dibagi menjadi 3 perlakuan (P1: kepadatan 0,1 m2, P2: kepadatan 0,18 m2, P3: kepadatan 0,24 m2). Analisis data menggunakan metode statistik non parametrik Kruskal Wallis. Kepadatan saat transportasi menyebabkan perbedaan persentase tingkah laku berdiri dan lokomosi juga durasi lokomosi domba Jonggol pasca transportasi. Tingkah laku berdiri pada kepadatan 0,18 m2 lebih rendah dibanding lainnya, namun mempunyai tingkah laku lokomosi yang lebih tinggi dibanding lainnya. Hal tersebut menunjukkan terjadinya tingkah stres yang tinggi pada kepadatan 0,18 m2., Stress in livestock due to transportation can be seen from the behavior of livestock after the transportation process. This study aimed to evaluate the differences in behavior of Jonggol sheep in 5 hours after long-distance transportation with different vehicle densities. The location of this research is at UP3J (Unit Pendidikan dan Penelitian Peternakan Jonggol) IPB and Small Ruminant Field Laboratory Block B, Faculty of Animal Science IPB. This study was conducted for 7 days in September 2022. This study used 27 Jonggol sheep divided into 3 treatments (P1: density 0.1 m2, P2: density 0.18 m2, P3: density 0.24 m2). Data were analyzed by Kruskal Wallis' non-parametric method. Overcrowding during transportation causes differences in the percentage of standing behavior and locomotion as well as the duration of locomotion of Jonggol sheep after transportation. The standing behavior at a density of 0.18 m2 is lower than others, but has a higher locomotion behavior than others. This showed the occurrence of high stress behavior at a density of 0.18 m2.
Keyword: domba, stress, tingkah laku, transportasi

Judul: The Sheep’s Physiological Response and Performance are Transported at Different Density After Long Distance Land Transmission
Abstrak: Pengangkutan domba merupakan kegiatan yang dapat menimbulkan stress pada domba yang ditunjukkan dengan penurunan bobot badan dan peningkatan respon fisiologisnya. Penelitian ini bertujuan untuk mengidentifikasi pengaruh kepadatan yang berbeda pada saat transportasi terhadap performa, respon fisiologis, lama waktu rekondisi serta analisis biaya. Penelitian ini menggunakan 10 domba betina dewasa dengan bobot 20,48±3,04kg dan diangkut dengan kepadatan 0,1 m2 ekor-1 dan 0,24 m2 ekor-1 selama 11 jam 30 menit. Variabel yang diamati meliputi susut bobot, respon fisiologis(suhu rektal, denyut jantung, dan laju respirasi), durasi waktu rekondisi serta analisis biayanya. Data dianalisis menggunakan Uji T tidak berpasangan untuk membandingkan rataan kedua kepadatan dan Uji T berpasangan untuk menilai kondisi domba sebelum dan setelah transportasi. Domba mengalami stress akibat perjalanan, hal ini ditandai dengan perubahan yang signifikan pada bobot badan dan respon fisiologisnya, namun kepadatan yang berbeda tidak berpengaruh nyata pada perubahan respon fisiologis dan waktu rekondisi domba. Domba yang diangkut dengan kepadatan 0,1 m2 ekor-1 lebih efisien secara ekonomi karena penggunaan biaya yang lebih rendah., Sheep transportation is an activity that may cause stress to the sheep, indicated by weight loss and changes in physiological response. The aim of this study was to investigate the effect of density level during transportation on performance, physiological response, recovery time, and cost analysis. This study used 10 female mature sheep with body weight 20,48±3,04kg and transporting with densities of 0,1 m2 head-1 and 0,24 m2 head-1 with long time transport journey 11 hour and 30 minutes duration. The observed variables include weight loss, physiological response(rectal temperature, heart rate, and respiratory rate), recovery time, and cost analys. Data were analysed using 2 sample T-test to compare of both density and using the paired T-test to analysed sheep condition before and after transportation. The sheep experienced stress due to transportation characterized by significant changes in weight loss and physiological response, but different of density level do not significantly affect changes in physicological response and recovery time. Sheep transported with density 0,1 m2 head-1 are more efficient with lower cost
Keyword: density, local sheep, performance, phisiological response, transportation

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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