Judul: Tingkah Laku Domba Garut Jantan Muda dengan Pemeliharaan Intensif yang Diberi Ransum Limbah Tauge pada Waktu Pemberian yang Berbeda
Abstrak: Research aimed to evaluate the effect of bean sprout waste as grass substitution and feeding time toward the ingestive, agonistic, and resting behaviour of the lamb. About 16 heads of lamb were maintained for 3 months with 2 treatments factors (feed and feeding time). The method was focal sampling and the datas were analysed using ANOVA. The result showed that there was no interaction between feed and feeding time toward the ingestive, agonistic, and resting behaviour. Feeding time gave significant effect (P<0.05) to the ingestive frequencies and agonistic duration. Morning feeding time gave higher consumption than afternoon feeding time meanwhile, afternoon feeding time gave higher effect for agonistic behaviour than morning feeding time.
Keyword: resting, ingestive, feeding time, feed, agonistic

Judul: Performa Domba Garut Jantan yang Diberi Pakan Limbah Tauge sebagai Pengganti Rumput Lapang pada Waktu Pemberian yang Berbeda
Abstrak: Research aimed to evaluate the effect of mung bean sprout waste as grass subtitution and different feeding time management (morning and evening) through the performance of Garut lamb in intensive system. About 16 heads of Garut lamb with the average body weight 15.06 ± 2.41 kg were analyzed in 2x2 factorial randomized complete block design. The first factor was different feed formula consist of R1 (consentrate 60% + grass 40%) and R2 (concentrate 40% + mung bean sprout waste 40%). The second factor was different feeding time consist of P (morning) and S (evening). The result of statistic analysis showed that R2 had significant effect (P<0.01) in DMI, CP, and TDN consumption. Different feed formula had significant effect (P<0.01) in ADG, so that the different feeding time and the interaction with different feed formula also had significant effect in ADG (P<0.05). Feed efficiency of R2 was higher (P<0.05) than R1. Mung bean sprout waste had better palatability than grass so that it enhanced the performance of Garut lamb with better feed efficiency.
Keyword: performance., mung bean sprout waste, garut lamb, feeding time

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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