Judul: Persepsi, Motivasi, Sikap Dan Perilaku Masyarakat Lokal Terhadap Keberadaan Hutan (Kasus Di Kecamatan Gn. Kencana, Kabupaten Lebak, Propinsi Banten)
Abstrak: A good forest management is an important part to get sustainable forest in Gn. Kencana where “Baduy Luar” and non Baduy in local community live. The aim of this research is to analyze perception, motivation, attitude and behavior from Baduy Luar and non Baduy community in Gn. Kencana about managing their forest in their area. This research use purposive sampling to get 30 respondent and 3 village sampling (Cimanyangray, Sukanegara and Keramatjaya) in Gn.Kencana . Perception for land use is for farming area. Perception for wood can get from quantity from that forest, closed standing stock, and diameter of trees. And then, for perception of water, it can get from periods water flow, and clear of water condition. The result, Baduy Luar communities have better perception than non Baduy about this forest. Local communities have motivation to subtantiate for life and to increase economic value from forest resources. Actually, both of them are agree to conserve the land forest, source of wood and water supply. We can look that action from a part of Baduy Luar community get source of wood from twig or natural died tree. Altough non Baduy community will cut the trees when they want. Local wisdom still be a part of Gn. Kencana community when they use natural resources (in this case is forest), especially for Baduy Luar. Keywords : Gn. Kencana, Perception, motivation, attitude and behavior
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Judul: Persepsi, Motivasi dan Perilaku Masyarakat Sekitar Hutan dalam Pengelolaan Kawasan Hutan (Kasus Kawasan Hutan sekitar Desa Gunung Sari di Kecamatan Pamijahan, Kabupaten Bogor)
Abstrak: The existence of forests to local communities surrounding the forest area gives economic, ecological, and social benefits. Empowering communities is the one of the important thing to preserve forests. The study assess the perceptions, motivations and behavior in using and managing forests. The method has been used was a structured interview (questionnaire) by taking a purposive sampling respondents, observation and literary study. The level of public perception for the existence of forest in the village of Gunung Sari is relatively good, whereas the public perception of the value of the economic functions of forests as sources of timber and additional income for poor households. Furthermore, the biggest motivation (35.48%) to manage forests based on the theory of Maslow (1943) was due to the need of security (safety needs), while the biggest behavior motivation based on the theory of Gerungan (1967) was group motif with a percentage of 32.26%. Behavior of people who used and managed the forest have been shown by utilizing water resources well, planted of forest trees, and didn’t convert land of the forest.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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