Judul: Pengoptimuman Fase Gerak KLT dengan Rancangan Campuran untuk Analisis Sidik Jari Temulawak
Abstrak: Temulawak is widely used as jamu, a traditional Indonesia medicine. Therefore we have developed a TLC fingerprint method to standardized raw material or extract of Curcuma xanthorrhiza to ensure the quality of its finished product. The fingerprint of TLC analysis is a common method for this purpose. First step to developed a TLC fingerprint was to select optimum mobile phase to obtained good separation of the components. Simplex centroid design is used for mobile phase optimization from three selected solvents that were acetone, dichloromethane, and chloroform. The results showed that the optimum mobile phase using UV 366 nm detection was obtained using dichloromethane and chloroform ratio of 0.5164:0.4836 with R2 value of 91.39%, whereas the optimum mobile phase using vanillin-sulfuric acid detection was obtained using dichloromethane and chloroform ratio of 0.8178:0.1822 with R2 value of 93.40%.
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Judul: Pengoptimuman Fase Gerak Kromatografi Cair Kinerja Tinggi untuk Analisis Sidik Jari Ekstrak Temu Putih (Curcuma zedoaria)
Abstrak: Curcuma zedoaria, known as temu putih in Indonesia is widely used as medicinal plant to treat some diseases and has been reported to have antimicrobial and antioxidant activities. For quality control of C. zedoaria, an attempt on fingerprint study was developed in this work. This study was aimed to determine the optimum condition of high performance liquid chromatography mobile phase for the fingerprint analysis of C. zedoaria collected from 3 different places, namely Cipaku, Cimanggu, and Dramaga and determine the antioxidant activities. The best eluent composition obtained was trifluoroacetic acid (TFA) 0.10% (A) and acetonitrile (B) by gradient elution method. The gradient program was started at 90% A:10% B, changed to 80% A:20% B in 12 minutes, and then to 40% A:60% B in 38 minutes, before finally brought back to the initial conditions in 10 minutes and held constant for 5 minutes. The mobile phase flow rate used was 1 mL·min-1. The highest inhibitory activity of 2,2-diphenyl-1-picrylhydrazyl stable radical was shown by the C. zedoaria extract collected from Dramaga with 50% inhibition concentration value of 182.01 μg·mL-1
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Judul: Kejadian Kasus Newcastle Disease (ND) pada Peternakan Ayam Buras di Kabupaten Barru Provinsi Sulawesi Selatan Tahun 2015–2017
Abstrak: Newcastle disease (ND) merupakan penyakit menular pada ayam dengan angka kematian yang tinggi. Kerugian ND dapat berupa kematian ayam dan penurunan produksi telur. Penelitian ini bertujuan menganalisis data kasus, mengukur kecepatan penyebaran penyakit, menggambarkan distribusi penyakit, dan memetakan wilayah yang berisiko terjadi penyebaran kejadian ND. Data penelitian diperoleh dengan cara wawancara mendalam menggunakan kuesioner terstruktur kepada pihak dinas. Data tersebut diolah dengan menghitung incidence rate dan memetakan wilayah berisiko menggunakan geographic information system (GIS). Hasil penelitian menunjukkan bahwa incidence rate tertinggi di 7 kecamatan selama 3 tahun adalah di Kecamatan Soppeng Riaja sebesar 14 kasus per 100 000 ekor—tahun, sedangkan incidence rate terendah adalah di Kecamatan Tanete Riaja sebesar 0 kasus per 100 000 ekor—tahun. Kecamatan dengan risiko tinggi adalah Kecamatan Soppeng Riaja, sehingga tindakan pengendalian perlu ditekankan pada daerah tersebut.
Keyword: Newcastle disease (ND), incidence rate, ayam buras, Kabupaten Barru