Judul: Kepadatan, Kesesakan, Privasi, Dan Kepuasan Lingkungan Keluarga Di Kota Bogor
Abstrak: Penelitian ini bertujuan untuk menganalisis pengaruh kepadatan, kesesakan, privasi terhadap kepuasan lingkungan keluarga di pemukiman marjinal Kota Bogor. Disain penelitian yang digunakan adalah cross sectional. Penelitian dilakukan di Kelurahan Balumbang Jaya, Kecamatan Bogor Barat dan Kelurahan Kebon Pedes, Kecamatan Tanah Sareal. Penelitian ini melibatkan 160 keluarga lengkap yang memiliki anak remaja yang dipilih secara simple random sampling. Contoh di masing-masing kelurahan sebanyak 80 keluarga lengkap yang memiliki anak remaja. Data dikumpulkan dengan teknik wawancara menggunakan kuesioner. Data dianalisis menggunakan uji korelasi, uji regresi, dan uji PLS. Hasil penelitian menunjukkan bahwa karakteristik lingkungan fisik keluarga dan kepadatan berhubungan dengan kepuasan lingkungan. Penelitian ini juga menemukan bahwa kepuasan lingkungan dipengaruhi oleh karakteristik lingkungan dan kepadatan. Hasil uji PLS menunjukkan bahwa karakteristik lingkungan fisik tempat tinggal dan kepadatan berpengaruh signifikan terhadap kepuasan lingkungan.
Keyword: karakteristik lingkungan fisik tempat tinggal, kepadatan, kesesakan, kepuasan lingkungan, privasi

Judul: Kepadatan, kesesakan, krivasi dan kesejahteraan subjektif keluarga di pemukiman marjinal Kota Bogor
Abstrak: Tujuan penelitian ini adalah menganalisis kepadatan, kesesakan, privasi dan kesejahteraan subjektif keluarga di pemukiman marjinal. Desain studi yang digunakan adalah cross sectional. Penelitian dilakukan di Kelurahan Babakan Pasar dan Kelurahan Paledang, Kecamatan Bogor Tengah, Kota Bogor dengan melibatkan 126 contoh yang memiliki anak usia 3-5 tahun dipilih secara simple random sampling. Hasil uji korelasi menunjukkanlama pendidikan istri, lama pendidikan suami, pendapatan perkapita dan jumlah ruang berhubungan positif signifikan dengan kesejahteraan subjektif. Lama menikah dan kesesakan berhubungan signifikan negatif dengan kesejahteraan subjektif. Terdapat tiga faktor yang berpengaruh terhadap kesejahteraan subjektif yaitu lama pendidikan istri, lama tinggal di wilayah tempat tinggal perpengaruh positif terhadap kesejahteraan subjektif sedangkan kesesakan berpengaruh negatif terhadap kesejahteraan subjektif., This research aimed to analyze the density, crowded, privacy and subjective well-being of marginalized settlement. The design of this study was Cross-sectional. This research was conducted in Sub-District Babakan Pasar, Paledang, Center Bogor. A total of 126 participants who have childrent from 3-5 years old were chosen using simple random sampling method. Result showed that wife and husban education length, per capita incomes and number of space, have positive correlation with subjective well-being. Familly size and crowded have negative significant correlation with subjective well-being. There were three factors influenced on subjective well-being these were wife education length and long time stayed at region that has positive influence, crowded has negative influence on subjective well-being.
Keyword: kepadatan, kesesakan, kesejahteraan subjektif, privasi

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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