Judul: Identifikasi Keragaman Gen Leptin Receptor (LEPR) pada Domba dan Hubungannya dengan Komposisi Asam Lemak Daging
Abstrak: LEPR merupakan gen yang termasuk dalam family reseptor sitokin dan berperan penting dalam regulasi metabolisme lemak. Penelitan ini bertujuan untuk mengidentifikasi keragaman gen LEPR dengan total sampel 47 domba, yaitu 20 sampel domba ekor gemuk (DEG), 17 sampel domba ekor tipis (DET) dan 10 sampel domba komposit garut (DKG) dan hubungannya terhadap asam lemak menggunakan teknik Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) dengan AciI sebagai enzim restriksi. Produk amplifikasi sepanjang 432 bp. Hasil keragaman gen LEPR pada semua populasi domba bersifat polimorfik dengan 3 genotipe yaitu AA, AC, dan CC. Uji Chi-khuadrat menunjukkan bahwa lokus LEPR berada dalam kesetimbangan Hardy-Weinberg pada populasi domba. SNP LEPR berasosiasi (P<0.05) dengan asam lemak tak jenuh jamak asam dokosaheksaenoat (C22:6n3) dan asam lemak jenuh termasuk asam trikosanoat (C23:0) dan asam tetrakosanoat (C24:0). SNP gen LEPR dapat digunakan sebagai penanda genetik untuk menseleksi domba yang memiliki kandungan asam lemak tak jenuh tinggi.
Keyword: asam lemak, domba, gen LEPR

Judul: Evaluasi Keragaman Gen Selenoprotein W (SEPW1) dan Asosiasinya terhadap Komposisi Asam Lemak pada Domba
Abstrak: Komposisi asam lemak dikontrol oleh beberapa gen, salah satu gen yang diduga memiliki peran dalam sifat ini adalah selenoprotein W (SEPW1). Penelitian ini bertujuan untuk mengidentifikasi keragaman gen SEPW1 dan asosiasinya dengan komposisi asam lemak pada domba. Total sampel yang digunakan sebanyak 55 ekor domba yang terdiri atas 20 domba ekor gemuk (DEG), 15 domba ekor tipis (DET), 10 domba komposit garut (DKG), dan 10 domba compass agrinak (DCA). Identifikasi keragaman SEPW1|BanI dianalisis menggunakan Polymerase Chain Reaction-Restriction Fragment Lenght Polymorphism (PCR-RFLP). Ukuran produk amplifikasi adalah 478 bp. Uji chikuadrat menunjukkan populasi yang diamati berada dalam keseimbangan Hardy- Weinberg. Keragaman gen SEPW1 ditemukan pada setiap rumpun domba. SNP (Single Nucleotide Polymorphism) g.53617063 G>A gen SEPW1 berasosiasi (P<0.05) dengan asam arakidat (C20:0) dan asam lemak total. SNP gen SEPW1 disimpulkan memiliki potensi untuk dikembangkan dalam pemuliaan ternak berbasis seleksi genomik untuk penentuan komposisi asam lemak.
Keyword: asam lemak, domba, gen SEPW1, PCR-RFLP

Judul: Thermal Characteristics and Dynamics of Tropical Cyclone over Western North Pacific Ocean: A Case Study of Typhoon Songda (Chedeng) 2011
Abstrak: The frequent occasion of Tropical Cyclone (TC) over Western North Pacific (WNP) Ocean lies from 10˚N to 26˚N and 121˚E to 170˚E. Sources of TC development come from warm ocean temperature where latent heat is released during condensation processes and subsequently concentrated in the boundary layer. Relative humidity at 850-700 hPa is responsible to produce the most intense storm. Meanwhile, weak vertical wind shear between 850-200 hPa is one of the dynamical factors that lead to the development of TC. Pressure level of 850-200 hPa is chosen to minimize the thermodynamic effect in the lower troposphere. Typhoon Songda (Chedeng in the Philippines) or TSC lasted from May 18th to 30th, 2011 and strengthened into powerful Super Typhoon stage between May 24th and 27th. Long curving track and the exhibition of all sequences of TC life cycle, TSC is then considered as an ideal case of TC. The objective of the study with case of TSC is to investigate the role of thermal characteristics and dynamics on TC intensity. The research is carried out by using Advanced Research of Weather Research and Forecasting model (WRF ARW model) version 3.3. Two domains are employed in the model. The first domain situate from 02˚N to 40˚N and 118˚E to 149˚E and cover spatial resolution () of 33.1 km. The second domain situate from 09˚N to 27˚N and 119˚E to 132˚E with of 11 km. Timestep of the model () is 120 seconds. The model is supported by the following physical parameterization, i.e., Yonsei University Scheme (YSU) in the boundary layer, Kain-Fritsch scheme (KF) in the cumulus, and WRF Single-Moment 3-class scheme in the microphysics. Initial meteorological condition prior to the formation of the cyclone is generated using WRF ARW standard-release model. Sensitivity of the model output is obtained from a selected single-parameter change scenarios, namely, 10 % to 20 % decrease and increase of wind shear initial data at 850-200 hPa, and by 10 % to 20 % decrease of relative humidity initial data at 850-700 hPa. The result showed that the negative correlations between wind shear and intensity are found in wind shear scenarios. Wind shears of 10 ms-1 which lead to strengthening of the wind intensity in the early period of Typhoon development are found in wind shear scenarios. The effect of wind shear changes on wind intensity is statistically significant in the 20 % wind shear scenarios. During mature stage, weak wind intensity occurred when wind shear increase as given in the WRF standard-release and the 20 % decreasing scenario. Different situation is found in the 20 % increasing scenario where wind shear tend to weak and lead to strong wind intensity at this stage. Meanwhile, the reduction of moisture supply at 850-700 hPa resulting from 20 % decreasing relative humidity play a major role in the weakening of wind intensity and reduces mechanical energy of the cycle process as much as 300 J kg-1.
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