Judul: Jenis dan Alokasi Waktu Kegiatan Anak Sekolah Dasar pada Sekolah Favorit dan Non Favorit di Kota Bogor
Abstrak: Penelitian ini bertujuan untuk mengetahui jenis dan alokasi waktu kegiatan anak Sekolah Dasar selama di sekolah pada anak di SD favorit dan SD non favorit, mengetahui jenis dan alokasi waktu kegiatan anak Sekolah Dasar selama di luar sekolah pada SD favorit dan SD non favorit, menganalisis pengeluaran energi anak dan tingkat kegiatannya serta menganalisis perbedaan proporsi anak dengan tingkat kegiatan ringan pada sekolah favorit dan non favorit.
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Judul: Keragaan Status Gizi, Aktivitas Fisik, Konsumsi Pangan Serta Tingkat Kecukupan Energi Dan Zat Gizi Anak Sekolah Dasar Di Kota Bogor
Abstrak: The objective of this research was to understand nutritional status, physical activity, food consumption and sufficiency energy and nutrient level of elementary school children in Bogor. The research was conducted by using cross sectional study and simple random sampling design from April to July 2009 in 4 elementary School in Bogor that 2 private school (SDIT Aliya and SD Pertiwi) and 2 public school (SDN Baranang Siang and SD Kedung Badak 1). The samples of this research were all of fifth grade students from four elementary school students in Bogor. The result showed that nutritional status obesity and overweight sample in private school higher than sample in public school. Physical activity level (PAL) of sample in private school category sedentary and sample in public school category active. Energy expenditure of sample in public school was higher than private school. Sample in private school has higher energy, protein, vitamin A, vitamin C, calcium, iron and phosphor consumption than sample in public school. Adequacy level of energy, protein, vitamin A, vitamin C, calcium and phosphor of private school sample were also higher than public school, except for iron. Pearson Correlation Test shows that physical activity has significant correlation with the nutritional status.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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