Judul: Aplikasi berbagai marka Sub1 (AEX1, SUB1C173, RM464A, RM 219) pada pembentukan dan seleksi F1 Ciherang toleran genangan.
Abstrak: Uncertain climate changes forced a tolerant rice abiotic stress, such as drought, high salinity, and submergence. Excessive rainfall often soak the rice fields which caused rice damage. Ciherang is a superior rice varieties that have a high productivity and resistance to some diseases. However, these rice varieties have a weakness that is sensitive submergence stress. Therefore, tolerant submergence rice varieties Ciherang is necessary to be developed. Rice which has a submergence tolerant gene (Submergence 1) has been known is donor-Sub1 rice (Swarna-Sub1 and IR64-Sub1). Insertion genes Sub1 from donor-Sub1 have done to Ciherang variety using site-directed crossing method. The objectives of this research’s were to build up and selecting F1 Ciherang-Sub1 rice plant that carried Sub1 gene with marker Sub1 using PCR technique. Result of this research showed that with the marker SUB1C173 obtained allele donor-Sub1 and F1 Ciherang-Sub1 sizes 108 bp and 126 bp. Results of selection using AEX1 marker obtained Sub1A allele with size 231 bp. RM219 marker can distinguish Ciherang’s host, Sub1- donor, and F1 Ciherang-Sub1 with DNA fragment sizes of 204 bp and 194 bp. But, RM464A marker cannot distinguish the difference of allele patterns from that three variety.
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Judul: Aplikasi PCR berbasis marka sub1 (AEX1 dan RM219) pada seleksi padi BC1F1 Ciherang-Sub1
Abstrak: Submergence stress is the third most important limitation to rice production from 42 biotic and abiotic stresses. FR13A has adapted to excessive flooding environment by submergence tolerance is controlled by a single major quantitative trait locus (QTL) on chromosome 9, named Sub1. However, these varieties are susceptible to diseases and insect pests. It is therefore an attractive research proposition to transfer Sub1 gene from traditional cultivars into more productive varieties. In this study, the Ciherang was used as host, and F1 Ciherang-Sub1 was used as donors Sub1. The purpose of this study were to generate BC1F1 Ciherang-Sub1 varieties from backcross between F1 Ciherang-Sub1 and Ciherang by site-directed crossing methods. Selection of BC1F1 was done by submergence test and PCR analysis based Sub1 marker. BC1F1 Ciherang-Sub1 was succed in producing and contain Sub1 gene are obtained 16 BC1F1 Ciherang/Swarna-Sub1 and 22 BC1F1 Ciherang/IR64-Sub1 plants. Results of PCR amplification based AEX1 showed DNA band for Sub1A gene at 231 bp. The rices were containing DNA band belong to submergence tolerance rices. RM219 marker could distinguished between Ciherang, Sub1 donors, and BC1F1 Ciherang-Sub1 with DNA fragment at 204 bp and 194 bp. However, the different is too small, it is ranges of 5-10 bp until difficult to determine clearly heterozygous of BC1F1 Ciherang-Sub1.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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