Judul: Preferensi Masyarakat terhadap Lanskap Visual Pemukiman (Studi Kasus Pemukiman di Kabupaten Cianjur dan Kotamadya Bogor)
Abstrak: Persepsi masyarakat terhadap lanskap pemukiman baik di perkotaan maupun di perdesaan yang masih kuat dipengaruhi oleh unsur tradisional sangat beragam Ole1h karena itu penelitian tentang persepsi dan preferensi masyarakat terhadap lanskap visual pemukiman perlu dilakukan. Tujuan penelitian ini adalah. mempelajarr preferensi masyarakat terhadap lanskap visual pemukiman perdesaan dan pemukiman perkotaan serta mencari unsur yang mempengaruhi persepsi dan preferensi masyarakat terhadap lanskap visual pemukiman.
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Judul: Hubungan persepsi masyarakat dan pemamfaatan taman lingkungan : Studi kasus Bukit Cimanggu Villa - Bogor
Abstrak: Tujuan penelitian ini untuk mengetahui keterkaitan antara persepsi masyarakat terhadap kondisi fisik dengan pemanfaatan taman lingkungan. Pemanfaatan yang dimaksud adalah berdasarkan ketersediaan fasilitas yang terdapat taman lingkungan. Selain itu, akan diketahui juga kaitan antara latar belakang responden yang meliputi jenis kelamin, umur, tingkat pendidikan, waktu luang dan status perkawinan dengan persepsi masyarakat terhadap kondisi fisik dan segi pemanfaatan taman lingkungan. Data yang diambil meliputi data primer dan data sekunder. Data primer dikumpulkan dari pembagian kuesioner, kepada potensial users sebanyak 50 orang. Metode yang dipakai dalam menyebar kuesioner adalah convenience sampling/purposive sampling secara non random sampling. Data primer yang didapat, kemudian saling ditabulasi-silangkan (cross tabulation). Untuk data mengenai latar belakang responden dianalisis secara deskriptif dengan menggunakan ukuran frekuensi/persentase. Guna mengetahui kaitan antara latar belakang responden dengan persepsi masyarakat terhadap kondisi fisik dan pemanfaatan taman lingkungan, dipakai cara tabulasi silang selain menggunakan uji kehomogenan khi-kuadrat Pearson sebagai bahan penguat. Data sekunder yang diambil meliputi denah lokasi, topografi, tanah, iklim, vegetasi, satwa serta kondisi umum taman lingkungan dan data lain yang menunjang proses penelitian. Data sekunder ini diperoleh dari Bagian Teknik PT. Perdana Gapura Prima selaku pengelola pemukiman Bukit Cimanggu Villa, Badan Meteorologi dan Geofisika Stasiun Dramaga Bogor, selain melalui pengamatan yang dilakukan di lapang. Sebagian besar responden terdiri dari wanita (70%), dengan kisaran usia dewasa (76%), sudah menikah dan mempunyai anak usia sekolah/Balita (40%) ..dst
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Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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