Judul: Pengaruh Menonton Film dan Bermain Video Game Kekerasan terhadap Perilaku Agresi Siswa SMP di Perdesaan Bogor
Abstrak: Menonton film dan bermain video game berunsur kekerasan memberikan dampak bagi peminatnya, salah satunya perilaku agresi. Penelitian ini bertujuan untuk menganalisis pengaruh menonton film dan bermain video game kekerasan terhadap perilaku agresi siswa SMP di perdesaan Bogor. Lokasi penelitian terletak di Desa Ciasihan dan Ciasmara, Kecamatan Pamijahan, Kabupaten Bogor. Penelitian ini menggunakan desain cross sectional study dengan melibatkan 60 siswa SMP kelas VII dan VIII yang memiliki orang tua lengkap yang dipilih secara proportional random sampling. Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan yang nyata pada film kekerasan, video game kekerasan dan perilaku agresi antara laki-laki dan perempuan. Uji korelasi menunjukkan bahwa menonton film dan bermain video game kekerasan berhubungan positif signifikan dengan perilaku agresi. Hasil uji regresi linear berganda menunjukkan bahwa menonton film dan bermain video game kekerasan memiliki pengaruh yang positif signifikan terhadap perilaku agresi.
Keyword: Agresi, Film kekerasan, Perdesaan, Remaja, Video game kekerasan

Judul: Pengaruh Perilaku Bermain Video Game Berunsur Kekerasan Terhadap Perilaku Agresi Remaja
Abstrak: Perkembangan teknologi saat ini begitu pesat, perkembangan ini mempengaruhi media audio visual salah satunya adalah video game. Tujuan dari penelitian ini yaitu: 1) Menganalisis perbedaan pengaruh tingkat bermain video game berunsur kekerasan terhadap tingkat perilaku agresi remaja, 2) Menganalisis perbedaan pengaruh faktor personal sebagai pembentuk perilaku bermain video game berunsur kekerasan terhadap tingkat perilaku agresi remaja, 3) Menganalisis perbedaan pengaruh faktor situasional sebagai pembentuk perilaku bermain video game berunsur kekerasan terhadap tingkat perilaku agresi remaja. Analisis dalam penelitian ini menggunakan tabulasi silang, uji statistik Mann-Whitney dan Kruskall-Wallis dengan taraf nyata 5%. Hasil penelitian menunjukkan bahwa terdapat perbedaan tingkat situasional dan perbedaan jenis kelamin terhadap tingkat perilaku bermain video game berunsur kekerasan; dan terdapat perbedaan tingkat perilaku bermain video game berunsur kekerasan terhadap tingkat agresivitas remaja.
Keyword: perkembangan teknologi, media audio visual, kekerasan dalam video game.

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable