Judul: Pengaruh status ekonomi keluarga terhadap motif menikah dini di Perdesaan
Abstrak: Pernikahan yang dilakukan pada perempuan di bawah umur berkaitan dengan kesiapan fisik maupun psikis yang belum mencapai kematangan termasuk pembentukan identitas diri maupun sosial individu sebagai remaja yang berada pada masa pencarian identitas. Tujuan penelitian ini adalah untuk mengidentifikasi motif-motif dan faktor yang melatarbelakangi pernikahan dini yang terjadi, serta menganalisis hubungannya terhadap pembentukan identitas. Penelitian ini dilakukan dengan menggunakan metode penelitian survei. Pengumpulan data dilakukan menggunakan teknik purposive sampling dengan jumlah responden sebanyak 30 orang. Pengujian pengaruh antara variabel faktor-faktor menikah dini terhadap motif menikah dini dilakukan dengan menggunakan uji regresi berganda, sedangkan variabel pembentukan identitas diuji melalui pendekatan kualitatif deskriptif. Hasil yang diperoleh menunjukkan bahwa pernikahan dini yang terjadi dilatarbelakangi oleh motif remaja untuk memenuhi kebutuhan akan keamanan, sosial, dan harga diri. Pembentukan identitas yang terbentuk pada remaja putri yang melakukan menikah dini ialah pembentukan identitas diri kuat dan pembentukan identitas sosial lemah., A marriage of under age women related with physical as well as psychological state of readiness which have not reached maturity include the formation of self identity and social identity as an adolescence that in fact are on the search for identity. The purpose of this research is to identify the motives and factors which aspects influenced early marriages that occurred, as well as analyzing its relationship towards the formation of identity. The research was carried out using survey research methods. Data collection was done using a purposive sampling technique with 30 respondents. Influence testing between variables factors of early married toward motives of early married conducted using test of multiple regression while formation of identity tested through by qualitatif description. The result showed that early marriage occurred by adolescence motives to fulfill the security, social, and self esteem. The identity formation related in adolescence who married early is strong formation self identity and weak formation social identity.
Keyword: Pernikahan dini, Faktor-faktor menikah dini, Motif menikah dini, Pembentukan identitas

Judul: Pengaruh Tekanan Ekonomi dan Interaksi Orangtua-Remaja Terhadap Motivasi Menikah Dini
Abstrak: Anak memiliki perlindungan di dalam hukum salah satunya terbebas dari pernikahan anak. Pernikahan yang terjadi pada usia anak memberikan dampak negatif terhadap tumbuh kembang anak dan keluarga yang dibentuk. Pernikahan anak tidak hanya disebabkan oleh faktor eksternal tetapi faktor internal remaja, seperti keinginan remaja untuk menikah dini. Penelitian ini bertujuan untuk mengetahui pengaruh tekanan ekonomi dan interaksi orang tua-remaja terhadap motivasi remaja untuk menikah dini. Penelitian ini menggunakan desain penelitian cross sectional dengan teknik voluntary sampling. Jumlah sampel penelitian sebanyak 165 remaja di Jabodetabek dan tinggal bersama kedua orang tua. Hasil analisis korelasi menunjukan bahwa tekanan ekonomi subjektif memiliki hubungan positif signifikan dengan motivasi menikah dini dan tingkat pendidikan ibu memiliki hubungan negatif signifikan dengan motivasi menikah dini. Hasil analisis pengaruh menunjukan tekanan ekonomi subjektif dan interaksi orang tua-remaja pada dimensi kekerasan dan kontrol psikologi berpengaruh positif signifikan terhadap motivasi menikah dini.
Keyword: child marriage, early marriage motivation, economic pressure, parent-adolescent interaction

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.