Judul: Perkecambahan Embrio Endospermik Mangga (Mangifera indica L.) Varietas Arumanis Klon 143 secara In Vitro
Abstrak: This study aimed to obtain the optimal medium for germination of endospermical embryos in mango (Mangifera indica L.) cv Arumanis clone 143. The effect of cold treatment and GA3 treatment had no significant difference in endospermical embryos germination. In the first experiment, embryos with 8 days cold treatment 83.3% of cotiledonary embryos produced the secondary proembryos with 33 secondary embryos. In the second experiment, the highest percentage of secondary proembryos (83.3%) we obtain in MS medium with 5 μM GA3. In the third experiment, the solid MS medium with 5 mg/l BA produced 80 secondary embryos, highest than in liquid medium with the similar composition, and 6.7% of that embryos germinated on solid medium. In the fourth experiment, the embryo medium induction has significant difference effect in embryo which produced by inoculum, whereas preculture medium had no significant difference effect. Inoculum from MS medium with 10 μM GA3 and cultured on PM preculture medium (MS medium with 5 mg/l 2,4-D, 5 mg/l GA3, 400 mg/l amino acid glutamine, 300 mg/l thrypton and 200 ml/l coconut water) produced embryo at 25.67 in 7 MST. The highest percentage of germinated embryos (32.4%) produced by inoculum from medium containing MS halfstrength major and minor nutrients with 5% coconut water, and then cultured on PM preculture medium.
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Judul: Induksi embriogenesis in vitro sel endosperma mangga (Mangifera indica L.) varietas arumanis klon 143
Abstrak: Mango Arumanis (Mangifera indica L) is a diploid plant with 2n=2x=40 chromosome. Embryogenesis from endosperm cells is the one opportunity to produce seedless triploid plant in mango Arumanis. The objective of this research are to obtain the optimum age of endosperm of mango seed for embryogenesis induction and to obtain the optimum medium for embryogenesis induction. This research conducted with 3 experiment are : (1) embryogenic callus induction from endosperm cells; (2) callus proliferation and (3) maturation of somatic embryo. Endosperm cells from different age of immature seed are : 1 week old, 2 weeks old and 3 weeks old from the initial fruit set was cultured in 10 medium compotition with several plant growth regulators and organic compound to induce embryogenic calli. Result of this research are : (1) Three weeks old endosperm cells from immature seed showed the best results for callus induction with percentage 26.28%, (2) Medium MA3 (B5 macro + MS micro + 0,2 mg/l BAP + 1 mg/l NAA + 0,5 mg/l GA3 + 3% sucrose) was the best medium for callus induction and somatic embryo development.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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