Judul: Penentuan Proporsi Alergi Produk Perikanan pada Mahasiswa Fakultas Perikanan dan Ilmu Kelautan IPB
Abstrak: Data penderita alergi produk perikanan di Indonesia masih terbatas, sehingga menyebabkan penanganan alergi tidak tepat. Penelitian ini bertujuan menentukan proporsi alergi produk perikanan pada mahasiswa Fakultas Perikanan dan Ilmu Kelautan (FPIK), Institut Pertanian Bogor (IPB). Survei berbasis daring dilakukan dengan menggunakan metode simple random sampling pada populasi mahasiswa FPIK, IPB angkatan 53 sampai dengan 56. Sebanyak 337 responden memberikan tanggapan dengan jumlah responden perempuan sebanyak 233 orang, dan jumlah responden laki-laki sebanyak 104 orang. Sebaran kelompok usia responden berada pada rentang 17 tahun sampai dengan 23 tahun. Total 48 responden dari 52 responden yang mengunjungi dokter spesialis didiagnosis memiliki alergi makanan. Responden perempuan dengan alergi makanan yaitu sebanyak 16,7%, dan responden laki-laki dengan alergi makanan yaitu sebanyak 8,7%. Gejala klinis alergi yang sering dijumpai yaitu reaksi kulit dan reaksi gastrointestinal. Hasil yang didapatkan menunjukkan bahwa kebanyakan gejala alergi dipicu oleh kelompok makanan laut.
Keyword: Allergy, allergen, seafood

Judul: Isolasi dan karakterisasi protein ikan tongkol (Auxis thazard), kerang hijau (Perna viridis) dan udang jerbung (Penaeus merguiensis) untuk pembuatan isolat alergen
Abstrak: Seafoods including fish, crustaceans and shellfish play an important role in human nutrition. However, it is also recognised as an important cause of food allergies, especially in coastal countries, including Indonesia. Treatments of these diseases still rely on avoiding the sources of the alergens, however it can also cause malnutrition which is also dangerous to health. To determine the specific food protein causing allergic reactions, cutie test was performed using isolate food protein. Until the present time, Indonesia still imports kits containing isolate proteins for food allergy diagnosis, therefore effort to locally produce the kit will reduce health cost for patients with allergic diseases. The objective of this study was to produce seafoods protein extract that is potential to be used as isolate of allergens for food allergy diagnosis. Two extract sarcoplasmic and myofibril protein were obtained from fish, shrimp and mussel by extraction in phosphate buffer with diference ionic strength Protein profile were then detected by means of sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE). IgE binding pattern was analyzed by using ELISA and immunoblotting using sera from 20 respondents with histories of food allergy. The result of the study showed that samples of seafood extract protein consist of several protein with various molecular weight in the range about 14 to 143 kDa. The component were identified as tropomyosin (34-38 kDa), parvalbumin (~12 kDa), myosin light chain (~17.5 kDa) and arginin kinase (40 kDa) which are major allergens in seafoods. Further analysis with ELISA showed that the seafood protein extracts contained several IgE-binding proteins. Immunoblot of three extract samples from seafoods showed that components identified as allergens to each allergic subject have different molecular weight. The results demonstrated that crude extract could be directly used as isolate allergen for diagnostic test allergy (skin prick test) without isolating the major allergen components.
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Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4