Judul: Ageism, generativitas, dan pencapaian tugas perkembangan lansia duda dan janda
Abstrak: Pada setiap tahap kehidupan manusia termasuk tahap usia lanjut (lansia) terdapat tugas perkembangan yang harus diselesaikan dan menuntut lansia untuk beradaptasi dengan berbagai perubahan baik dari dalam diri sendiri maupun dari lingkungan sosial. Penelitian ini bertujuan untuk menganalisis pengaruh ageism dan generativitas terhadap tugas perkembangan lansia duda dan janda. Desain penelitian yang digunakan adalah cross-sectional study. Penelitian dilakukan di Kota Bogor tepatnya di Kelurahan Empang, Kecamatan Bogor Selatan dan Kelurahan Cilendek Barat, Kecamatan Bogor Barat. Sebanyak 60 orang dilibatkan sebagai contoh penelitian, terdiri dari 30 orang lansia duda dan 30 orang lansia janda yang dipilih dengan teknik snow-ball. Hasil penelitian menunjukkan tidak terdapat perbedaan nyata skor pencapaian tugas perkembangan lansia duda dan janda. Ageism berada pada kategori rendah sedangkan generatvitas dan tugas perkembangan pada kategori sedang. Terdapat hubungan dan pengaruh positif signifikan lama pendidikan dan generativitas terhadap tugas perkembangan lansia duda dan janda.
Keyword: Ageism, Elderly widow, Elderly widower, Developmental task, Generativity, Snowball method, Latter adulthood

Judul: Pemenuhan perkembangan tugas keluarga serta kepuasan pernikahan pada lansia laki-laki dan perempuan
Abstrak: Penelitian ini bertujuan mengidentifikasi perkembangan tugas keluarga serta kepuasan pernikahan lansia laki-laki dan perempuan. Desain penelitian yang digunakan adalah cross sectional study. Penelitian bertempat di Kecamatan Bogor Barat khususnya Kelurahan Pasir Jaya, Cilendek Barat, dan Menteng. Pengambilan contoh dilakukan secara purposive dengan kriteria berada pada masa lansia awal dan berstatus menikah. Responden yang terlibat berjumlah 62 orang dengan 31 orang berjenis kelamin laki-laki dan 31 orang berjenis kelamin perempuan. Penelitian ini menggunakan Kuesioner Perkembangan Tugas Keluarga Lansia dan Marital Satisfaction Questionnaire for Older Persons. Usia dan jumlah anggota keluarga berhubungan negatif dengan perkembangan tugas keluarga. Pendapatan keluarga berhubungan positif dengan perkembangan tugas keluarga. Responden laki-laki memiliki kepuasan pernikahan yang lebih tinggi dibandingkan perempuan. Variabel pola tempat tinggal dan perkembangan tugas keluarga mempengaruhi kepuasan pernikahan., The aim of this research was to analyze family developmental tasks and marital satisfaction of older persons. A cross sectional study was used as a design of this study. The research took place in District West Bogor, particularly in three Sub-Districts, those were Pasir Jaya, Cilendek Barat, dan Menteng. Participants in this research were taken purpossively by characteristics such as categorized as early elderly and married. The amount of participants in this research were 62, 31 male and 31 female. This research used Family Aging Couple Developmental Tasks Questionnaire and Marital Satisfaction Questionnaire for Older Persons. Age and amount of member’s family were negatively correlated with family developmental tasks. Family’s monthly income was positively correlated to family developmental tasks. Male participants had higher score of marital satisfaction than female participants. The pattern of the older persons live and family developmental tasks was influence towards marital satisfaction.
Keyword: kepuasan pernikahan, lansia awal, pensiunan, perkembangan tugas keluarga, pola tempat tinggal

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable