Judul: Validation of Microsatellite Molecular Markers on Several Santol Plants (Sandoricum koetjape Merr.)
Abstrak: Tanaman kecapi tergolong dalam famili Meliaceae yang banyak dibudidayakan di Asia Tenggara, terutama di Indonesia dan Thailand. Aksesi tanaman kecapi sulit dibedakan secara morfologi, sehingga perlu dilakukan pendekatan berbasis molekuler menggunakan marka mikrosatelit. Penelitian ini dilakukan di Laboratorium Seed Center Departemen Agronomi dan Hortikultura Institut Pertanian Bogor pada bulan September 2022 hingga Mei 2023. Pada penelitian ini digunakan 16 aksesi tanaman kecapi masing-masing dari lokasi yang berbeda. Uji kuantitas dan kualitas DNA 16 aksesi kecapi menggunakan spektrofotometer dihasilkan konsentrasi DNA berkisar antara 100,6 hingga 786,6 ng/µL. Kemurnian DNA berkisar antara 1,41 hingga 2,14 pada A260/A280 dan 0,38 hingga 1,97 pada A260/A230. DNA masing-masing aksesi diamplifikasi menggunakan 10 primer mikrosatelit, namun hanya aksesi BGR5, BNT, IPB3 yang dapat teramplifikasi. Hal tersebut menunjukkan primer Sk1 hingga Sk10 masih perlu divalidasi., The santol plant belongs to the Meliaceae family which is widely cultivated in Southeast Asia, especially in Indonesia and Thailand. It is difficult to distinguish morphologically between santol plant accessions, so it is necessary to do a molecular-based approach using microsatellite markers. This research was conducted at the Seed Center Laboratory of the Department of Agronomy and Horticulture, Bogor Agricultural University from September 2022 to May 2023. In this study, 16 accessions of the santol plant were used, each from a different location. Testing the quantity and quality of DNA of 16 santol accessions using a spectrophotometer resulted in DNA concentrations ranging from 100.6 to 786.6 ng/µL. DNA purity ranged from 1.41 to 2.14 for A260/A280 and 0.38 to 1.97 for A260/A230. The DNA of each accession was amplified using 10 microsatellite primers, but only the BGR5, BNT, IPB3 accessions could be amplified. This shows that the primers Sk1 to Sk10 still need to be validated.
Keyword: Santol, microsatellite, amplification, genetic

Judul: Vegetative Growth Response of Kecapi (Sandoricum koetjape Merr.) Seedlings to The Different NPK Fertilizer Composition in Foliar Application
Abstrak: Kecapi (Sandoricum koetjape Merr.) atau biasa dikenal dengan nama santol merupakan salah satu tanaman penting dalam famili Meliaceae. Kecapi memiliki potensi yang sangat besar untuk dikembangkan karena memiliki beberapa fungsi dan manfaat, namun tanaman ini sudah jarang ditemukan. Salah satu upaya yang dapat dilakukan ialah meningkatkan pertumbuhan pada tahap pembibitan melalui pemupukan. Salah satu metode pemupukan ialah secara foliar yaitu dengan cara menyemprotkan pupuk pada permukaan daun. Penelitian dilakukan berdasarkan Rancangan Kelompok Lengkap Teracak (RKLT) dengan satu faktor. Faktor tunggal adalah pengaplikasian jenis pupuk NPK yang terdiri atas lima taraf yaitu A0 (kontrol), A1 (pupuk NPK 15:15:15), A2 (pupuk NPK 32:10:10), A3 (pupuk NPK 15:30:15), dan A4 (pupuk NPK 8:9:39) dengan volume semprot masingmasing 110 ml/tanaman. Hasil penelitian menunjukkan perlakuan bahwa komposisi pupuk NPK 8:9:39 paling berpengaruh tinggi diantara perlakuan lainnya kecuali pada parameter jumlah daun dan indeks luas daun. Nilai pertambahan tinggi tanaman, diameter batang, dan tebal daun pada perlakuan tersebut sebesar 3,10 mm; 0,074 mm; dan 115,10 µm. Sementara kandungan klorofil a dan b sebesar 66,04 µg ml-1 dan 25,15 µg ml-1, serta kandungan glukosa dan fruktosa pada daun sebesar 0,177 µg ml-1 dan 0,044 µg ml-1. Namun, pada parameter jumlah daun nilai yang lebih tinggi terdapat pada perlakuan pupuk NPK 15:15:15 sebesar 15,3 helai daun. Indeks luas daun perlakuan kontrol memiliki nilai yang lebih tinggi sebesar 0,65., Kecapi (Sandoricum koetjape Merr.) or commonly known as santol is one of the important plants in the Meliaceae family. Kecapi has enormous potential to be developed because it has several functions and benefits but it is rarely found. One of the efforts that can be done is increasing growth in nurseries through fertilization. One of various fertilization methods is foliar fertilization, namely by spraying fertilizer on the leaf surface. The study was conducted based on a Completely Randomized Block Design (RKLT) with one factor. The single factor was the application of NPK fertilizer which consisted of five levels, namely A0 (control), A1 (NPK fertilizer 15:15:15), A2 (NPK fertilizer 32:10:10), A3 (NPK fertilizer 15:30:15), and A4 (NPK fertilizer 8:9:39) with each spray volume is 110 ml/plant. The results showed that the composition of NPK 8:9:39 had the highest effect among other treatments, except for the number of leaves and leaf area index. The 8:9:39 NPK fertilizer treatment had the highest increase in plant height, stem diameter, and leaf thickness of 3.10 mm; 0.074 mm; and 115.10 µm. The contents of chlorophyll a and b were 66.04 g ml-1 and 25.15 g ml-1. The content of glucose and fructose in the leaves was 0.177 g ml-1 and 0.044 g ml-1. However, the parameter number of leaves treated with NPK 15:15:15 had a higher value of 15.3 leaves. The leaf area index of the control treatment had a higher value of 0.65.
Keyword: chlorophyll, composition, foliar fertilizer, fructose, glucose

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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