Judul: The Interrelation between Stress Level, Quality of Diet, Nutritional Status, and Academic Achievement of Students, The Interrelation between Stress Level, Quality of Diet, Nutritional Status, and Academic Achievement of Students
Abstrak: Konsumsi pangan yang beragam dan bergizi merupakan hal yang penting bagi status gizi normal serta fungsi otak agar dapat memiliki prestasi belajar yang baik. Penelitian ini bertujuan menganalisis keterkaitan tingkat stres, kualitas diet, status gizi, dan prestasi belajar SMA. Penelitian cross-sectional ini melibatkan 82 subjek yang dipilih secara purposive. Pengumpulan data tingkat stres dilakukan melalui pengisian kuesioner oleh subjek, kualitas diet diestimasi menggunakan Indonesian Healthy Eating Index (I-HEI), berat badan dan tinggi badan diukur secara langsung untuk mengestimasi status gizi. Sebagian besar subjek memiliki tingkat stres tinggi (62,2%), kualitas diet dengan kategori membutuhkan perbaikan atau need improvement (58,6%), dan status gizi normal (62,2%). Tidak terdapat hubungan signifikan (p>0,05) antara tingkat stres dengan kualitas diet (p=0,320, r=-0,111) dan prestasi belajar (p=0,236, r=0,039); antara kualitas diet dengan status gizi (p=0,618, r=0,056); serta antara status gizi dengan prestasi belajar (p=0,180, r=0,150). Namun demikian, seluruh subjek yang gemuk (overweight dan obes) memiliki kualitas diet yang tidak baik (buruk dan membutuhkan perbaikan). Subjek dengan prestasi belajar baik cenderung memiliki status gizi baik (60,9%) dan tingkat stres tinggi (56,5%). Hal ini mengindikasikan bahwa subjek dengan status gizi baik lebih berpeluang memiliki prestasi belajar yang baik pula., The consumption of varies and nutritious foods is important for normal nutritional status and brain function to improve academic achievement. This study aimed to analyze the correlation between stress levels, the quality of diet, nutritional status, and high school academic achievement. This cross-sectional study involved 82 subjects selected by purposively. Stress level data was collected through filling out questionnaires by subjects, diet quality was estimated by Indonesian Healthy Eating Index (I-HEI), body weight and height were measured directly to estimate nutritional status. Most subjects had high level of stress (62,2%), diet quality with the category need improvement (58,6%), and normal nutritional status (62,2%). There was no significant correlation (p>0,05) between stress levels with diet quality (p=0,320, r=-0,111) and academic achievement (p=0,236, r=0,039); between diet quality with nutritional status (p=0,618, r=0,056); between nutritional status with academic achievement (p=0,180, r=0,150). However, all subjects who were overweight and obese had either poor or need improvement their diet quality. Subjects with good academic achievements tended to have a good nutritional status (60,9%) and high stress levels (56,5%). This indicated that subjects with good nutritional status were more likely to have a good academic achievement as well.
Keyword: Academic Achievement, Diet Quality, Nutritional Status, Stress Level

Judul: The Correlation between Stress Score, Eating Habit and Nutritional Status of Final Year Sudents
Abstrak: Mahasiswa tingkat akhir sering mengalami stres karena mengerjakan skripsi, sehingga dapat memengaruhi pola makan dan status gizi. Penelitian ini bertujuan menganalisis hubungan nilai skor stres, pola makan dan status gizi pada mahasiswa tingkat akhir. Desain penelitian menggunakan studi cross sectional melibatkan 30 subjek. Penelitian dilakukan November 2019 hingga Maret 2020 di Fakultas Ekologi Manusia IPB. Data yang dikumpulkan adalah karakteristik individu dan keluarga, nilai skor stres, pola makan, dan status gizi. Data nilai skor stres dikumpulkan menggunakan kuesioner Depression Anxiety Stress Scales 42 (DASS 42), sedangkan data pola makan dikumpulkan menggunakan Food Frequency Questionnaire (FFQ) dan recall 2 x 24 jam. Hasil penelitian menunjukkan pola makan sebagian besar subjek belum sesuai panduan gizi seimbang. Sebagian besar subjek memiliki rata-rata tingkat kecukupan gizi defisit, 83,3% subjek berstatus gizi normal dan 63,3% subjek memiliki nilai skor stres terkategori stres sedang. Terdapat hubungan signifikan negatif antara nilai skor stres dengan asupan energi (p<0,05), artinya semakin tinggi nilai skor stres mahasiswa, asupan energi subjek semakin rendah. Tidak terdapat hubungan pola makan dengan status gizi (p<0,05), demikian pula tidak terdapat hubungan antara nilai skor stres dengan status gizi (p>0,05)., Final-year students often experience stress because they work on their thesis, which may affect their eating habits and nutritional status. This study analyzes the relationship between stress scores, eating habits, and final year students' nutritional status. The study used a cross-sectional study design involving 30 subjects. The research was conducted from November 2019 to March 2020 at the Faculty of Human Ecology, IPB. The data collected were individual and family characteristics, stress scores, eating habits, and nutritional status. Stress score data were collected using the Depression Anxiety Stress Scales 42 (DASS 42) questionnaire while eating habit data were collected using the Food Frequency Questionnaire (FFQ) and 2x24-hour recall. The results showed that the diet of most of the subjects did not comply with balanced nutrition guidelines. Most of the subjects had an average nutritional adequacy deficit, 83,3% of subjects with normal nutritional status, and 63,3% had stress score categorized as moderate stress levels. There is a significant negative relationship between the stress score and energy intake (p <0,05), meaning that the higher the students stress score the lower the energy intake. There is no relationship between eating habits and nutritional status (p<0,05), as well as no relationship between stress scores and nutritional status (P>0,05).
Keyword: final year students, eating habit, nutritional status, stress score

Judul: Isolasi Konstruksi dan Rekombinasi Gen Pengkode Reverse Transcriptase asal Simian Retrovirus-2 (SRV-2)
Abstrak: The availability of reverse transcriptase enzym as a valuable component for Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique has been providing by foreign companies. Therefore, it needs more cost and time to provide it. This study is an early step to produce in house reverse transcriptase rekombinant enzym from SRV-2. The purpose of this study was isolated, constructed, and rekombined SRV-2 reverse transcriptase gene to entry clone and destination vector. Isolation was done by PCR amplification technique using three pairs of primers, which are RT I (3282-3683), RTII (3261-3900), and RTIII (3169-4133). Construction and recombination in this study used Gateway method from Invitrogen. SRV-2 reverse transcriptase gene was isolated in this study with A-549 cell inoculated SRV-2 as source of virus. This gene amplify by PCR technique and resulted specific band for RTI 401 bp, RTII 639 bp, and RTIII 964 bp. Analysis of pENTR/SD/TOPO and pDEST17 indicated SRV-2 reverse transcriptase gene code has already inserted to this plasmid. This result indicated SRV-2 reverse transcriptase gene have isolated, constructed, and recombined to entry clone and destination vector.
Keyword: