Judul: Hubungan Sedimen Tersuspensi dan Kehilangan Tanah Menurut Metode "Usle" di Sub Das Citarum Nanjung
Abstrak: Sub DAS Citarum Naqjung merupakam. salah aatu dari 22 DAS superprioritas di Indonesia. Suh DAS ki jugz sangat penting lraregn d i hilir alixan sungainya hanyaK dibangun waduk besar g a i t u wad* Saguling, C i r a t a dan Jatiluhur. Karena i t u , kehilangan tanah d i sub DAS i d rerupakila rasalah peating, tidak h w a karena mengurangi kesuhuran tanah, tetapi juga hasil sedimentasinyaa mempengaruhi unsur waduk.
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Judul: Hubungan karakteristik sosial tingkat kemiringan tanah dengan keragaan usahatani konservasi pada lahan kering: studi kasus DAS Riau Kanan Propinsi Kalimantan Selatan
Abstrak: Daerah Aliran Sungai (DAS) Riam Kanan merupakan sumberdaya alam yang mempunyai nilai strategis untuk menunjang pembangunan wilayah di Kalimantan Selatan, oleh sebab itu kelestariannya perlu dijaga agar fungsi sebagai pembangkit tenaga listrik dan pemasok air untuk irigasi dapat berkelanjutan. Karena fungsinya yang strategis (sebagai pembangkit tenaga listrik dan pemasok air irigasi Riam Kanan) maka DAS Riam Kanan ditetapkan sebagai DAS super prioritas, dan jika terjadi gangguan terhadap fungsi DAS perlu mendapat penanganan serius dan segera. Penelitian ini bertujuan untuk mengetahui: a). hubungan antara karakteristik sosial dan tingkat kemiringan tanah dengan keragaan usahatani konservasi tanah, b). upaya-upaya yang dilakukan petani dalam mengatasi masalah degradasi lahan…dst
Keyword: usahatani, lahan kering, DAS, degradasi lahan

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.