Judul: The Behavior of Jonggol Sheep for 5 Hours After Long-Distance Transportation with Different Densities
Abstrak: Stres pada ternak akibat transportasi dapat dilihat dari perilaku ternak setelah proses transportasi. Penelitian ini bertujuan untuk mengevaluasi perbedaan tingkah laku domba Jonggol pada 5 jam pasca transportasi jarak jauh dengan kepadatan kendaraan yang berbeda. Lokasi penelitian ini berada di UP3J (Unit Pendidikan dan Penelitian Peternakan Jonggol) IPB dan Laboratorium Lapangan Ruminansia Kecil Blok B, Fakultas Peternakan IPB. Penelitian ini dilaksanakan selama 7 hari pada September 2022. Penelitian ini menggunakan 27 ekor domba Jonggol yang dibagi menjadi 3 perlakuan (P1: kepadatan 0,1 m2, P2: kepadatan 0,18 m2, P3: kepadatan 0,24 m2). Analisis data menggunakan metode statistik non parametrik Kruskal Wallis. Kepadatan saat transportasi menyebabkan perbedaan persentase tingkah laku berdiri dan lokomosi juga durasi lokomosi domba Jonggol pasca transportasi. Tingkah laku berdiri pada kepadatan 0,18 m2 lebih rendah dibanding lainnya, namun mempunyai tingkah laku lokomosi yang lebih tinggi dibanding lainnya. Hal tersebut menunjukkan terjadinya tingkah stres yang tinggi pada kepadatan 0,18 m2., Stress in livestock due to transportation can be seen from the behavior of livestock after the transportation process. This study aimed to evaluate the differences in behavior of Jonggol sheep in 5 hours after long-distance transportation with different vehicle densities. The location of this research is at UP3J (Unit Pendidikan dan Penelitian Peternakan Jonggol) IPB and Small Ruminant Field Laboratory Block B, Faculty of Animal Science IPB. This study was conducted for 7 days in September 2022. This study used 27 Jonggol sheep divided into 3 treatments (P1: density 0.1 m2, P2: density 0.18 m2, P3: density 0.24 m2). Data were analyzed by Kruskal Wallis' non-parametric method. Overcrowding during transportation causes differences in the percentage of standing behavior and locomotion as well as the duration of locomotion of Jonggol sheep after transportation. The standing behavior at a density of 0.18 m2 is lower than others, but has a higher locomotion behavior than others. This showed the occurrence of high stress behavior at a density of 0.18 m2.
Keyword: domba, stress, tingkah laku, transportasi

Judul: Effect of Density on Physiological Responses and  Performance of Local Sheep on Long Distance Transport
Abstrak: Pengangkutan domba menjadi faktor yang tidak dapat dihindari dalam rantai pasok dan kepadatan menjadi salah satu penyebab stres selama transportasi. Penelitian bertujuan menganalisis pengaruh tingkat kepadatan selama transportasi terhadap respon fisiologis, penyusutan bobot badan, dan durasi rekondisi domba lokal, serta analisis biayanya. Penelitian menggunakan 10 ekor domba lokal betina dewasa. Penelitian dilakukan dengan mengangkut domba dengan kepadatan 0,18 m2 ekor-1 dan 0,24 m2 ekor-1 dengan transportasi jarak jauh berdurasi 11 jam 30 menit. Variabel yang diamati meliputi respon fisiologis (suhu rektal, denyut jantung, dan laju respirasi), susut bobot, durasi rekondisi, dan analisis biaya. Data dianalisis menggunakan Uji T berpasangan untuk menilai kondisi domba sebelum dan setelah perlakuan dan Uji T tidak berpasangan untuk membandingkan rataan kedua kepadatan. Domba mengalami stres akibat transportasi ditandai dengan perubahan yang signifikan pada respon fisiologis dan susut bobotnya. Tingkat kepadatan tidak mempengaruhi perubahan suhu rektal, denyut jantung, laju respirasi, susut bobot, dan durasi rekondisi domba lokal. Domba yang diangkut menggunakan tingkat kepadatan 0,18 m2 ekor-1 lebih efisien secara ekonomi karena penggunaan biaya yang lebih rendah., Sheep transportation is an unavoidable factor in the supply chain and density is one of the stressors during transportation. The aim of this study was to analyse the effect of density level during transportation on physiological responses, body weight loss, recovery of local sheep, and cost analys. The study used 10 female and mature local sheep. The study was conducted by transporting sheep with densities of 0,18 m2 head-1 and 0,24 m2 head-1 with long journey transport of 11 hours 30 minutes duration. The observed variables included physiological responses (rectal temperature, heart rate, and respiratory rate), weight loss, recovery, and cost analys. The data were analyzed using the paired T test to analyse sheep condition before and after the treatment and 2 sample T test to compare of both densities. Sheep experienced stress due to transportation characterized by significant changes in physiological responses and body weight lost. Density level didn’t affect changes in rectal temperature, heart rate, and respiratory rate, weight loss, and recovery duration of local sheep. Sheep that transported by density level 0,18 m2 head-1 economically are more efficient with lower cost.
Keyword: domba lokal, kepadatan, performa, respon fisiologis, transportasi

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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