Judul: Kajian Komunitas Lamun di Perairan Pesisir Manokwari
Abstrak: Study of seagrass community in coastal water of Manokwari was done in July-October 2007. The location (sampling points) consisted of seagrass beds that were closed to anthropogenic waste and/or suspended particles sources (i.e. Andai, Wosi, and Briosi) and seagrass beds that were relatively far from those sources (i.e. Rendani and Tj. Mangewa). Seagrass samplings were conducted by line transect and plot methods, environmental parameters were measured. There were eight species collected which included in proineer group (C. rotundata, H. pinifolia, H. uninervis, H. ovalis, S. isoetifolium) and climax group (C. serrulata, E. acoroides, T. hemprichii).
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Judul: Kajian komunitas padang lamun sebagai fungsi habitat ikan di perairan Manokwari Papua Barat
Abstrak: Secara ekologis ekosistem padang lamun di perairan pesisir dapat berperan sebagai daerah perlindungan ikan-ikan ekonomis penting seperti ikan baronang dan penyu, menyediakan nutrien ke perairan sekitarnya, memiliki fungsi sebagai produsen primer, pendaur ulang zat hara, sebagai habitat biota, tempat memijah ikan, mencari makan berbagai biota laut, stabilisator dasar perairan, perangkap sedimen, penahan erosi dan dapat memproduksi 10 gr bahan kering daun per hari. Pada habitat padang lamun hidup berbagai macam spesies hewan, yang berasosiasi dengan padang lamun, sebagai contoh di perairan teluk Ambon ditemukan 48 famili dan 108 jenis ikan yang adalah sebagai penghuni lamun, di Teluk Banten ditemukan 360 spesies, Pulau Bintan 33 jenis ikan dari 22 famili dan 72 jenis dari 39 famili ikan yang berasosiasi dengan lamun di teluk Awur Jepara. Kondisi ekosistem padang lamun di perairan pesisir Indonesia telah mengalami kerusakan sekitar 30%-60%. Kerusakan padang lamun di Manokwari berada dalam status kurang kaya dan miskin dengan prosentase 14-45% tahun 2008 dan 1-16% tahun 2009. Perairan Manokwari yang memiliki jenis kekayaan laut yang tinggi seperti lamun dan ikan yang tersebar di sepanjang perairan laut yang perlu dilestarikan karena dapat memberikan kontribusi pada peningkatan hasil perikanan dan sektor pariwisata. Namun penelitian dan informasi mengenai ekosistem ini masih sangat terbatas. Mengingat pentingnya peranan lamun dan ikan yang hidup didalamnya bagi ekosistem perairan laut dan semakin banyaknya tekanan dari aktivitas manusia maupun secara alami, maka perlu diupayakan pengelolaan yang baik dan berkelanjutan.
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Judul: Antibodi anti-idiotipe sebagai kandidat vaksin rabies, Anti-idiotype andibody as a rabies vaccine candidate
Abstrak: Anti rabies serum (ARS) called antibody 1 (Ab1) was used as the antigen to immunize laying chickens. Anti-Ab1 chicken immunoglobulins (IgY), the antiidiotype antibodies against rabies (Ab2) was isolated from chicken blood every week for ten weeks. The highest titer of Ab2 was found at the third week after the last immunization. Immunoglobulin Y was separated by means of ammonium sulfate precipitation, then dialyzed using PBS pH 8.0 for 24 hours at 2 – 8 oC and purified using affinity chromatography column for IgY lead the proteins molecules with 185,000; 95,000 and 49,000 dalton in weigth. Purified IgY was used to immunize New Zealand White rabbit and the antibody response (Ab3) was detected using Agar Gel Precipitation Test (AGPT). The Ab3 showed specific reaction with Ab2 as well as rabies virus. Interestingly, Ab2 and rabies virus express partial identity reaction. This lead the insight, that not all parts of Ab2 are internal image of rabies virus. The efficacy of Ab3 was detected using enzyme linked immunosorbent assay (ELISA) and indicated that the level of Ab3 titer is protective according to WHO standard (more than 0.5 IU/ml). The conclusion of this study is the anti-idiotype antibody can induce protective immune response against rabies virus and can be used as an alternative for rabies anti-idiotype vaccine. Key words : anti-idiotype vaccine, antibody, rabies
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