Judul: Koefisien Absorpsi dan Atenuasi Cahaya di Permukaan Air Laut pada Berbagai Musim
Abstrak: Pengukuran koefisien absorpsi dan atenuasi cahaya dapat berguna dalam memperkirakan kualitas perairan, karakteristik optik perairan, serta digunakan dalam permodelan bio-optik kelautan. Penelitian ini bertujuan untuk mengetahui nilai dan variabilitas koefisien absorpsi dan atenuasi permukaan laut di Perairan Timur Laut Teluk Meksiko berdasarkan perbedaan musim. Penelitian dilakukan dengan menggunakan data hasil survei pada tiga musim (semi, panas, dan gugur) antara tahun 1999 dan 2000 dengan menggunakan instrumen AC-9. Pola distribusi spasial dan nilai rata-rata antar wilayah menunjukkan bahwa koefisien absorpsi dan atenuasi secara umum relatif tinggi di sekitar Mississippi dan Mobile, kemudian relatif rendah di sekitar wilayah offshore. Variabilitas yang terjadi disebabkan karena besarnya pengaruh fitoplankton, CDOM, dan detritus terhadap koefisien absorpsi dan atenuasi, terutama di sekitar wilayah Mississippi dan Mobile. Nilai rata-rata koefisien menunjukkan bahwa secara umum koefisien absorpsi dan atenuasi di Perairan Timur Laut Teluk Meksiko relatif tinggi pada musim panas tahun 1999 (Su-99) dan relatif rendah pada musim semi tahun 2000 (Sp-00).
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Judul: Profil Koefisien Diffuse Atenuasi Kolom Perairan di Timur Laut Teluk Meksiko
Abstrak: Process of reduction light by scattering and absorption processes is known as attenuation. The aim of this research was to determine the attenuation coefficient of water column in the northeastern Gulf of Mexico. The field data collection was conducted in the northeastern Gulf of Mexico in April 2000. Downwelling irradiance (Ed) data were used to calculate attenuation coefficient (Kd). Data were filtered by using Curve Fitting Tool. The location of this research was divided into four locations: A, B, C, and D. The results showed that Kd in site A ranged from 0.039 - 0.137 m-1, in location B ranged from 0.026 - 0.060 m-1, in location C ranged from 0.015 - 0.036 m-1, and in location D ranged from 0.014 - 0.049 m-1. Distribution of Kd values increased from blue to red region. However, in the green region showed lower values. Result also showed that Kd values between study sites were significantly different (Kruskal-Wallis test, α=0.05). This result indicated that water column characteristic among locations were significantly different
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Judul: Isolasi Konstruksi dan Rekombinasi Gen Pengkode Reverse Transcriptase asal Simian Retrovirus-2 (SRV-2)
Abstrak: The availability of reverse transcriptase enzym as a valuable component for Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique has been providing by foreign companies. Therefore, it needs more cost and time to provide it. This study is an early step to produce in house reverse transcriptase rekombinant enzym from SRV-2. The purpose of this study was isolated, constructed, and rekombined SRV-2 reverse transcriptase gene to entry clone and destination vector. Isolation was done by PCR amplification technique using three pairs of primers, which are RT I (3282-3683), RTII (3261-3900), and RTIII (3169-4133). Construction and recombination in this study used Gateway method from Invitrogen. SRV-2 reverse transcriptase gene was isolated in this study with A-549 cell inoculated SRV-2 as source of virus. This gene amplify by PCR technique and resulted specific band for RTI 401 bp, RTII 639 bp, and RTIII 964 bp. Analysis of pENTR/SD/TOPO and pDEST17 indicated SRV-2 reverse transcriptase gene code has already inserted to this plasmid. This result indicated SRV-2 reverse transcriptase gene have isolated, constructed, and recombined to entry clone and destination vector.
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