Judul: Keragaman Gen CSN3 Ekson 4 pada Kambing Peranakan Etawah di BPTU KDI-HPT Pelaihari Kalimantan Selatan
Abstrak: Etawah grade goat as one of Indonesian local animal genetic resources has advantages, one of which is its high milk yield. CSN3 gene is a member of casein fragment related to protein and casein contents. The aim of this study was to identify polymorphisms of CSN3 in Etawah grade goat from BPTU KDI-HPT Pelaihari, South Kalimantan using the PCR-RFLP method. Polymorphisms of CSN3 gene were identified of 115 blood samples using PstI restriction enzyme that cuts the fragment of CTGCA|G. CSN3 amplification resulted 407 bp which is located at exon 4 was performed with annealing temperature 60 °C for 20 seconds. The results of PCR-RFLP analysis indicated that the analyzed CSN3 gene of Etawah grade goat which have monomorphic F allele was 100%.
Keyword: PCR-RFLP, Etawah grade goats, CSN3 gene

Judul: Eksplorasi Gen Growth Hormone Exon 3 Pada Kambing Peranakan Etawah (PE), Saanen Dan Pesa Melalui Teknik PCR-SSCP
Abstrak: This research was conducted to identify genetic polymorphism at the exon 3 growth hormone gene in three goat breeds. Polymorphisms at exon 3 growth hormone gene was identified by single strand conformational polymorphism polymerase chain reaction (SSCP-PCR) method. The DNA of 234 goat used were Etawah Grade (98 samples), Saanen (92 samples) and their crossbred (44 samples) in Cariu, Ciapus, Sukajaya, Cijeruk, Balitnak and Sukabumi. The PCR-SSCP method was performed at 250 V for 8 hours using 12% of acrylamide concentration. The result showed that the annealing temperature is 60 0C. The PCR product was 157 bp (base pair). The result SSCP method found four conformational patterns. The genotype frequency in exon 3 are AA (0,205), AB (0,856), AC (0,163) and BC (0,045). Beside that it was found three allele (allele A, B, and C). The highest frequencies were allele A (0,602) and B (0,443) at Saanen, Etawah Grade and their crossbred goat. The highest genotype frequency was AB at three goat breeds. The highest heterozygosity was found in Etawah Grade, Saanen and their crossbred goat (0,938). Exon 3 GH gene on three breeds goat have high polymorphism in six population.
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Judul: Pengukuran Engagement pada Permainan Orbital-X, Permainan Pertahanan Menara Sirkular
Abstrak: Jenis permainan pertahanan menara (tower defense) adalah sebuah gaya permainan yang memiliki tantangan tersendiri untuk menyusun sebuah strategi melawan musuh dengan menempatkan menara pertahanan yang tepat. Permainan dalam tugas akhir ini adalah permainan pertahanan menara yang memiliki konsep berbeda, yaitu dengan menaruh menara pertahanan di posisi tengah dan menara lainnya berputar sirkula. Sebelum permainan disebarkan, dibuat 2 buah kuesioner, yaitu kuesioner pra-permainan dan pasca permainan untuk mengukur engagement pemain. Kuesioner pra-permainan diberikan kepada 5 orang. Kemudian, mereka diberi waktu untuk memainkan Orbital-X selama satu minggu. Setelah itu, kelima responden diberikan kuesioner pasca permainan untuk mengukur engagement ketika bermain. Hasil pengukuran engagement pada aspek immersion, pemain sangat setuju dengan pertanyaan “Saya dapat dengan mudah menggunakan kontrol Orbital-X dan saya dapat menguasainya dengan cepat” dan pemain sangat tidak setuju dengan pertanyaan “Interaksi yang terjadi dalam dunia game yang saya mainkan sama halnya seperti saya berinteraksi di dunia nyata”.
Keyword: Engagement, Gameplay, Immersion, Permainan, Pertahanan Menara