Judul: Performa Benih Ikan Nila yang Diberi Pakan Mengandung Hormon Pertumbuhan Rekombinan dengan Metode Penyiapan Berbeda
Abstrak: This research was conducted to evaluate the influence of rElGH in the form of the bacterium Escherichia coli intact and contains whole culturing medium, E.coli containing rElGH without culture medium, and protein total of lysozymelysated E. coli and mixed with feedstuffs before it made into pellet (repeleting) on red Nile tilapia juvenile. The research consisted of 3 treatments by different preparation of rElGH at a dose of 3 mg/kg diet, and one control. Each treatment was repeated three times. The control diet and rElGH was firstly coated by chicken egg yolks,and then mixed with diet by repelleting. Fish were reared for 6 weeks and fed diet containing rElGH by 3 times at day 1, 4 and 7 of the first week, 10% of fish body weight. The result showed that feeding fish by diet containing lysozyme-lysated rElGH generated higher growth, biomass and specific growth rate (SGR) of length and body weight compared with the control. Increment of body weight, biomass and SGR of length and body weight were 8.30%, 7,39% 4.20% and 4,92% higher compared to the control, respectively. Thus, feeding fish with diet containing the lysated rElGH is an effective method to increase growth of red Nile tilapia juvenile.
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Judul: Efektivitas Pemberian Hormon Pertumbuhan Rekombinan Melalui Pakan dengan Bahan Penyalut Berbeda dan Pelleting pada Ikan
Abstrak: Application of recombinant growth hormone (rGH) to improve the growth of fish can be performed by injection, immersion and oral delivery method through feed. However, injection and immersion methods are relatively difficult to be applied to mass scale. Injection delivery method is time consuming and labor intensive. Furthermore, immersion method is only effective on the larval stage or small fish size, at a certain immersion dose and frequency. In addition, applications mass-scale immersion method with frequency of more than 1 time, high-potential causing fish stress. Oral delivery method is practical and time efficient. However, by this method rGH need to be coated to avoid degradation by digestive enzymes, and damage due to the low pH in the stomach. Hydroxypropyl methylcellulose phthalate (HP55) is a material often used for coating the rGH, but it is relatively expensive and inefficient when applied in large amounts. Another cellulose such as polymethyl carbamide (PMC) can also be used as a coating agent. PMC is a relatively cheap price, and has been reported to protect the coated material in a relatively long time; storage at a temperature of 20oC for about 1 year, 25 to 30oC about 6 months, and 35 to 38 °C about 3 months. Chicken egg yolk has also been widely used as a binder of medicines and feed supplements in fish. Its price is relatively cheap and easy to obtain. This study was performed to examine the effectiveness supplementation of recombinant giant grouper GH (rElGH) in tilapia feed using a variety of coating materials and pelleting on production, feed conversion, blood glucose, glycogen levels and proximate content of fish. Production of rElGH was performed using Escherichia coli BL21 containing pCold-ElGH protein expression vector. Bacteria were cultured in 2xYT media at incubation temperature of 15°C for 24 hours and protein synthesis was induced with isopropyl-bD-thiogalac-topyranoside in a dose of 0.5 mM. Lysis of bacterial cell walls was conducted using lysozyme, then total protein in inclusion body of rElGH were collected by centrifugation. SDS-PAGE analysis was used to ensure the presence of rElGH in the total bacterial protein. This study consisted of 5 treatments including the control, and each treatment was given 3 replications. The size fish used fish was 5 to 6 cm in body length. Fish were maintained at hapa installed in a concrete pond (measuring 3x2x1 m) with a stocking density of 125 fish in each hapa. Fish maintenance was carried out for 2 months. During the first 2 weeks fish were fed diet without rElGH (feed adaptation) with a frequency of 3 times a day, and then fed on rGH-supplemented diet in 3 times at intervals of 3 days. The results showed that chicken egg yolk coated rGH treatment generated significantly biomass (P<0.05) compared to the control (1262.2 ± 96.4 g). Fish biomass among treatments was similar (P>0.05). Increase in biomass in chicken egg yolk coating was 46.85%, 32.50% by HP55, 27.99% by PMC, and 26.64% by pelleting method compared with the control. In addition, specific growth rate (SGR) of fish treated by HP55, chicken egg yolk, and PMC coated rGH
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Judul: Penggunaan Teorema Homeomorphy 2-Manifold dan Teorema Euler Poincare pada Torus 𝑇�� dan Simplicial Complex 𝐾��
Abstrak: Two dimensional topological spaces are said to be homeomorphic if they have the same topological invariant, where one of topological invariant used is an Euler characteristic. Homeomorphy 2-Manifold’s Theorem and Euler Poincare’s Theorem are used to distinguish two topological spaces. Homeomorphy 2-Manifold’s Theorem uses Euler characteristic to identify two dimensional topological spaces. Euler Poincare’s Theorem is an alternative way to find Euler characteristic with Betti number, which is topological invariant as well. The objective of this paper is to investigate the homeomorphism of torus 𝑇����� and simplicial complex 𝐾�����. Topological space torus and simplicial complex 𝐾����� have the same Euler characteristic, which is two. Based on Homeomorphy 2-Manifold’s Theorem topological space torus 𝑇����� and simplicial complex 𝐾����� is homeomorphic.
Keyword: simplicial, torus, Euler, homeomorphism, topology