Judul: Regeneration of Adventitious Shoots in In Vitro Culture of Kitolod (Hippobroma longiflora (L.) G.Don)
Abstrak: Kitolod (Hippobroma longiflora (L.) G.Don) merupakan tumbuhan liar yang banyak dimanfaatkan oleh masyarakat sebagai obat tradisional. Bila pemanfaatan tanaman ini semakin berkembang, dikhawatirkan akan terjadi kekurangan ketersediaan sumber benih. Penelitian ini bertujuan mendapatkan teknik dan kondisi kultur terbaik dalam perbanyakan tanaman obat kitolod secara in vitro agar tersedia bibit yang dapat digunakan oleh masyarakat secara luas. Metode penelitian menggunakan rancangan acak lengkap (RAL) dengan dua faktor perlakuan dan 10 ulangan untuk semua percobaan kecuali pengakaran tunas adventif. Daun dan petiol kitolod digunakan sebagai bahan eksplan. Eksplan daun pada perlakuan BAP 1 mg/L + NAA 0,1 mg/L memberikan jumlah tunas adventif per eksplan paling tinggi yaitu sampai 49 tunas, namun perlakuan BAP 2 mg/L + NAA 0,1 mg/L lebih efektif dalam inisiasi dan perbanyakan tunas kitolod. Tunas-tunas pada BAP 2 mg/L + NAA 0,1 mg/L juga mampu menghasilkan tunas tertinggi (2,62±0,69 cm) pada media pembesaran dan memberikan 75% keberhasilan dalam tahap aklimatisasi. Media pengakaran terbaik adalah media MS yang ditambah NAA 0,5-1,0 mg/L., Kitolod (Hippobroma longiflora (L.) G. Don) is a wild plant that widely used by the community as a traditional medicine. If this plant is utilized more intensively, there may be a shortage of the plant due to the lack of the seed sources. This study aimed to obtain the best techniques and culture conditions for in vitro propagation of kitolod to provide a large number of planting materials for the community. The experiments were arranged using a completely randomized design (CRD) with two treatment factors and 10 replications for all experiments except shoot rooting. Leaves and petioles were used as explant sources. Leaf explants in 1 mg/L BAP + 0,1 mg/L NAA treatment produced the highest number of adventitious shoots per explant, up to 49 shoots, but 2 mg/L BAP + 0,1 mg/L NAA was more effective for shoot initiation and multiplication. The latter medium was also able to produce the tallest shoots (2,62±0,69 cm) and presented 75% of successful rate over the acclimatization period. The best rooting medium was MS medium added with 0,5-1,0 mg/L NAA.
Keyword: organogenesis, planting materials, tissue culture, traditional medicine

Judul: Penggunaan Beberapa Jenis Sitokinin Terhadap Multiplikasi Tunas dan Pertumbuhan Binahong (Anredera cordifolia [Ten.] Steenis) secara In Vitro
Abstrak: Biological diversity in Indonesia occupied third place in the world that using to fulfill needs of basic necessities and supply of medicines. Utilization of medicinal plant in domestic region become larger in a row with improvement of people awareness to consumed natural medicines. Binahong (Anredera cordifolia [Ten.] Steenis) is one of medicinal plant that have a potention to expand as medicine basic commodity. It also useful to treat any diseases and motivated some researchers to examine their bioactive substances. This examination also need research about multiplication using tissue culture technique. Application various of single cytokinin in different concentration is expected able to influenced shoot multiplication and growth of binahong to keep this plant preservation. The research consist of two experiments that first experiment is monitoring effect of sterilization treatment to contamination level, browning and death. Whereas second experiment is monitoring about the effect media treatment in various of cytokinin (AdSO4, BAP, kinetin and thidiazuron) with different concentration (0,50; 1,00; 1,50; and 2,00) mg/l to shoot multiplication and growth of binahong. In second experiment compiled using RAL statistic method with 10 repetations. Parameters are shoot amount (adventif and lateral shoot), height increment, leaf amount, root amount and callus process. In sterilization treatment shows addition of 70 % alcohol during 3 minutes gived highest success percentage (92,76 %). Lowest success percentage happend in control sterilization treatment (46,99 %). Shoot multiplication and growth of binahong shows in 1 MST. This research produced two various of shoots which is adventif shoot ang lateral shoot. Average great amount of shoots produced by 1,50 mg/l kinetin treatment (2,10 adventif shoot) and 1,50 mg/l BAP (3,90 lateral shoot). Control media not produced both of shoots. MS media with addition 0,50 mg/l kinetin showed average height increment and the best amount of leaves are 4,33 cm and 4,70 sheet. In every treatment shows root formation with greatest number produced by control media (8,80 roots). Callus formed in great part of explants that planted in treatment media except in AdSO4 (0,50; 1,00; and 2,00) mg/l and control media. Conclusion of this research is addition various of cytokinin with different concentration in MS media have obvious influental to the parameters.
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Judul: Metrik Einstein (Anti-) Self-Dual Berdimensi Empat dengan Metode Cartan
Abstrak: Gravitational field equation can be formulated in differential forms (differential geometry), which describes gravity as a manifestation of the curvature of space-time. Curvature of space-time is characterized by a metric that satisfies the gravitational field equation. With differential forms it can be formulated the (anti-) self-dual Einstein equation, which is a modification of the Cartan structure in (anti-) self-dual basis. Calculation of (anti-) self-dual metric with (anti-) self-dual Einstein equation is simpler than the calculation using Christoffel symbols.
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