Judul: Ketahanan Benih Udang Vaname Litopenaeus vannamei SPF (Specific Pathogen Free) Terhadap Infeksi Buatan TSV (Taura Syndrome Virus)
Abstrak: White shrimp culture had suffered from losses due to the outbreak of TSV in which mortality could achieved 95%. Therefore SPF shrimp is required as a preventive effort. This research was conducted to evaluate the resistance of white shrimp seed obtained from several SPF broodstock to TSV. Infection of TSV was carried out by feeding flesh of TSV-infected shrimp to PL 30 for 2 days at a feeding rate of 50%. After that, shrimp was fed commercial diet at 10% feeding rate till the end of experiment (11 days of culture). Parameters observed included survival rate, clinical symptoms, and weigth gain. After 11 days of culture, all the tested shrimps were infected, showing clinical symptom namely black spot on the body and the tail became red. The highest survival rate was obtained at S6 code (77,33±3,51%), while the lowest was observed at S4 code but statistically was different significantly (P<0,05) compared to other.
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Judul: Survival of Vaname Shrimp Given Recombinant Viral Protein Inhibitor Through Feed With Different Frequency Against WSSV Infection
Abstrak: White spot syndrome virus (WSSV) merupakan patogen yang sangat sering menginfeksi udang. Penelitian ini bertujuan menguji efektivitas rekombinan viral inhibitor protein (VIP) melalui pakan dengan frekuensi berbeda terhadap tingkat kelangsungan hidup udang vaname yang diuji tantang dengan WSSV. Penelitian ini dilakukan pada bulan Agustus-Oktober 2022 di Labroratorium Marikultur, analisis respons imun di Laboratorium Reproduksi dan Genetika Ikan, Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor. Penelitian ini dilaksanakan secara eksperimental menggunakan rancangan acak lengkap. Penelitian dilakukan menggunakan empat perlakuan dengan lima ulangan untuk uji tantang. Parameter uji yang diukur yakni tingkat kelangsungan hidup (TKH), total haemocyte count (THC), dan aktivitas phenoloxidase (PO). Data diolah menggunakan Microsoft Excel 2010 dan dianalisis dengan analisis ragam (ANOVA). Hasil penelitian ini menunujukkan bahwa pemberian VIP dengan frekuensi setiap hari memberikan hasil terbaik setelah diinfeksikan dengan WSSV yang menghasilkan tingkat kelangsungan hidup mencapai 45,33% dibandingkan (P<0,05) kontrol positif yang memiliki tingkat kelangsungan hidup terendah yakni 9,33%., White spot syndrome virus (WSSV) is a pathogen that often infects shrimp. This study aims to test the effectiveness of recombinant viral inhibitor protein (VIP) by feeding with different frequencies on the survival rate of vannamei shrimp challenged with WSSV. This research was conducted in August-October 2022 at Mariculture Laboratory, immune response analysis at Fish Reproduction and Genetics Laboratory, Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University. This research was carried out experimentally using a completely randomized design. The study was conducted using four treatments with five replications for the challenge test. The test parameters measured were survival rate (TKH), total haemocyte count (THC), and phenoloxidase (PO) activity. Data were processed using Microsoft Excel 2010 and analyzed by analysis of variance (ANOVA). The results of this study showed that giving VIP with a daily frequency gave the best results after being infected with WSSV which resulted in a survival rate of 45.33% compared to (P<0.05) the positive control which had the lowest survival rate of 9.33%.
Keyword: lethal dose, recombinant, viral inhibitory protein, WSSV

Judul: Peranan Pemimpin Informal dalam Menggerakan Partisipasi Masyarakat  untuk Pembangunan Desa
Abstrak: Pendekatan pembangunan yang banyak diterima para ahli adalah pendekatan dari bawah ke atas (bottom up) yang berarti bahwa dalam pembangunan diperlukan keikutsertaan rakyat. Keikutsertaan tidak hanya pada tahap merencanakan pembangunan, akan tetapi juga ikut dalam melaksanakan, bahkan sampai pada menilai hasil-hasil pembangunan (Cohen dan Uphoe 1977; Dusseldorp, 1981; Slamet, 1993). Agar rakyat yang berhimpun dalam kelompok-kelompok dapat ikut serta, mereka harus memiliki sejumlah kualifikasi yang diperlukan untuk itu.
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