Judul: Relationship between Physical Activity, Hydration Status, Diet Quality and Fitness of Dramaga Muda Basketball Club Athletes, Bogor Regency, Relationship between Physical Activity, Hydration Status, Diet Quality and Fitness of Dramaga Muda Basketball Club Athletes, Bogor Regency
Abstrak: Penelitian ini bertujuan menganalisis hubungan antara aktivitas fisik, status hidrasi, kualitas diet dan kebugaran pada atlet basket Club Dramaga Muda Kabupaten Bogor. Desain penelitian ini adalah cross sectional study menggunakan purposive sampling dengan jumlah 34 subjek. Data status gizi ditentukan dengan indikator z-score IMT/U dan sebagian besar tergolong status gizi baik (76,5%). Data aktivitas fisik subjek diukur menggunakan Phisycal Activity Level (PAL) dengan rata-rata aktivitas tergolong ke dalam kategori berat pada hari latihan dan ringan pada hari non latihan. Data status hidrasi didapat dengan metode Periksa Urin Sendiri (PURI) dan sebagian besar subjek terhidrasi dengan baik. Data kualitas diet diperoleh dari konversi food recall 2x24 jam dan diukur menggunakan Diet Quality Index for Adolescent (DQI-A). Skor DQI-A subjek (57,2%) terkategori ke dalam kuartil 3. Data kebugaran subjek didapat melalui Multi Stage Fitness Test (MFT). Tingkat kebugaran subjek laki-laki (42,9 ± 4,2) dan perempuan (32,5 ± 3,6) tergolong cukup. Ditemukan hubungan yang signifikan dan searah antara aktivitas fisik dengan kebugaran subjek (p = 0,011; r = 0,430). Kualitas diet dan kebugaran subjek juga memiliki hubungan signifikan yang searah (p = 0,006; r = 0,460). Namun, tidak ditemukan hubungan antara status hidrasi dengan kebugaran subjek (p>0,05)., This study aims to analyze the relationship between physical activity, hydration status, diet quality and fitness in basketball athletes at the Dramaga Muda Club, Bogor Regency. The design of this research was a cross sectional study using purposive sampling with a total of 34 subjects. Nutritional status data was determined using the BMI for age z-score indicator and the majority were classified as good nutrition (76.5%). The subject's physical activity data was measured by Physical Activity Level (PAL) with average activity grouped into vigorous on exercise days and light on non exercise days. Hydration status data was obtained using the Self-Urine Check (PURI) method and most subjects were well hydrated. Diet quality data was obtained from 2x24 hour food recall conversion and measured using the Diet Quality Index for Adolescent (DQI-A). The subject's DQI-A score (57.2%) was categorized into quartile 3. Subject fitness data was obtained through the Multi Stage Fitness Test (MFT). The fitness level of male subjects (42.9 ± 4.2) and female subjects (32.5 ± 3.6) was categorized as fair. A significant and unidirectional relationship was found between physical activity and the subject's fitness (p = 0.011; r = 0.430). Diet quality and subject fitness also had a significant relationship (p = 0.006; r = 0.460). However, no relationship was found between hydration status and subject fitness (p>0.05).
Keyword: DQI-A, basketball, fitness, hydration status, physical activity

Judul: Hubungan Pengetahuan Gizi, Konsumsi Pangan, dan Aktivitas Fisik terhadap Kebugaran Atlet Bola Basket di SMAN 4 Padangsidimpuan
Abstrak: Tujuan dari penelitian ini adalah untuk menganalisis hubungan antara pengetahuan gizi, konsumsi pangan, dan aktivitas fisik terhadap kebugaran atlet bola basket di SMAN 4 Padangsidimpuan. Penelitian ini menggunakan desain cross sectional study yang melibatkan 22 orang siswa, terdiri dari 10 orang laki- laki dan 12 orang perempuan. Tempat penelitian dipilih secara purposive dengan pengambilan subjek secara purposive sampling. Penelitian dilaksanakan pada bulan Juli-Agustus 2015. Skor pengetahuan gizi contoh pada umumnya berada dalam kategori sedang (68.2%). Sebagian besar (77.3%) status gizi contoh berada dalam kategori normal. Tingkat kecukupan energi, protein, dan karbohidrat pada umumnya berada dalam kategori defisit berat, sedangkan tingkat kecukupan lemak tergolong normal. Sebagian besar (77.3%) aktivitas fisik contoh berada dalam kategori sedang dan nilai VO2max sebagian besar (72.2%) contoh berada dalam kategori kurang. Hasil penelitian menunjukkan terdapat hubungan yang signifikan antara jenis kelamin (p=0.037, r= -0.448), status gizi (p=0.032, г-- 0.457), dan aktivitas fisik (p=0.042, r=0.437) dengan kebugaran. Tidak terdapat hubungan yang signifikan antara usia, berat badan, tinggi badan, pengetahuan gizi, dan tingkat kecukupan energi, protein, lemak, dan karbohidrat dengan kebugaran (p>0.05).
Keyword: Fitness, Nutrients adequacy level, Nutritional knowledge, Physical activity, Human ecology, Cross sectional study, Basketball athletes, Status gizi, Aktivitas fisik

Judul: Isolasi Konstruksi dan Rekombinasi Gen Pengkode Reverse Transcriptase asal Simian Retrovirus-2 (SRV-2)
Abstrak: The availability of reverse transcriptase enzym as a valuable component for Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique has been providing by foreign companies. Therefore, it needs more cost and time to provide it. This study is an early step to produce in house reverse transcriptase rekombinant enzym from SRV-2. The purpose of this study was isolated, constructed, and rekombined SRV-2 reverse transcriptase gene to entry clone and destination vector. Isolation was done by PCR amplification technique using three pairs of primers, which are RT I (3282-3683), RTII (3261-3900), and RTIII (3169-4133). Construction and recombination in this study used Gateway method from Invitrogen. SRV-2 reverse transcriptase gene was isolated in this study with A-549 cell inoculated SRV-2 as source of virus. This gene amplify by PCR technique and resulted specific band for RTI 401 bp, RTII 639 bp, and RTIII 964 bp. Analysis of pENTR/SD/TOPO and pDEST17 indicated SRV-2 reverse transcriptase gene code has already inserted to this plasmid. This result indicated SRV-2 reverse transcriptase gene have isolated, constructed, and recombined to entry clone and destination vector.
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