Judul: Pemanfaatan ruang terbuka untuk permainan-anak tradisional di Desa Baginda, Kecamatan Sumedang Selatan, Kabupaten Sumedang
Abstrak: Ruang terbuka di Kabupaten Sumedang mengalami penurunan luasan. Padahal, salah satu fungsi dari ruang terbuka ialah sebagai ruang bermain anakanak. Tujuan penelitian ini ialah mengkaji seberapa besar pemanfaatan ruang terbuka untuk permainan-anak tradisional di Desa Baginda dan mencari kebutuhan luasan untuk jenis permainan tradisional tersebut. Pengumpulan data dilakukan dengan penyebaran kuisioner kepada 30 responden anak-anak dan 30 responden orangtua. Kuisioner tahap satu disebar untuk mencari informasi mengenai persepsi masyarakat mengenai kegiatan bermain di ruang terbuka, khususnya untuk permainan tradisional. Selain mencari persepsi, dicari pula preferensi mengenai jenis permainan tradisional yang diketahui dan masih digemari oleh masyarakat di Desa Baginda. Kuisioner tahap dua mencari informasi mengenai kebutuhan luasan untuk masing-masing permainan yang dihasilkan dari penyebaran kuisioner tahap satu. Hasil yang diperoleh menunjukkan bahwa masyarakat desa Baginda memiliki pengetahuan mengenai permainan tradisional Sunda dan memiliki keinginan untuk bermain permainan tersebut di ruang terbuka. Namun, terdapat beberapa faktor penghambat, seperti kurangnya data atau informasi mengenai permainan tradisional, tidak adanya pihak yang mengajarkan, dan tidak adanya ruang bermain anak yang dibuat secara khusus oleh pihak desa. Ruang terbuka yang biasanya digunakan untuk bermain anak-anak berupa lapangan, sawah, dan lahan kosong. Di Desa Baginda terdapat ruang terbuka berupa lapangan sepak bola yang dapat dimanfaatkan untuk kegiatan pameran atau lomba permainan tradisional. Kedua kegiatan tersebut dapat dilakukan pada peringatan hari-hari besar dan tidak bersifat permanen. Hal tersebut mempertimbangkan fungsi utama lapangan ruang terbuka yang ada untuk berolahraga. Selain itu, hasil yang diperoleh menunjukkan bahwa masyarakat desa Baginda tidak mengenali seluruh jenis permainan tradisional Sunda yang diakui oleh Dinas Pariwisata dan Kebudayaan Jawa Barat. Terdapat 16 jenis permainan tradisiona Sunda yang dikenal dan masih digemari hingga saat ini di Desa Baginda. Setelah memperoleh data jenis permainannya, dilakukan pencarian informasi mengenai kebutuhan ruang untuk bermain permainan tersebut dengan menyebar kuisioner tahap dua kepada responden yang sama. Hasilnya, lahan yang dibutuhkan untuk mengakomodasi 16 jenis permainan tersebut adalah 607 meter². Permainan tersebut adalah bebeletokan (30 m²), bedil jepret (35 m²), bekel (6 m²), congklak (6 m²), egrang/jajangkungan (53 m²), galah asin (126 m²), hong 25/ucing peungpeun/ucing sumput (63 m²), ketepel/bandring (67 m²), lompat karet (25 m²), ngadu karbit (51 m²), ngadu muncang (11 m²), oray-orayan (28 m²), sapintrong (21 m²), sondah/engklek (16 m²), sur-ser (7 m²), dan susumputan (62 m²). Hasil akhir dari penelitian ini berupa rekomendasi desain ruang terbuka dengan fasilitas permainan tradisional Sunda.
Keyword: ruang bermain anak, ruang terbuka, permainan tradisional

Judul: Rencana Pengelolaan Lanskap Pertanian Desa Pangulah Utara, Kecamatan Kota Baru, Kabupaten Karawang, Jawa Barat, sebagai Sarana Bermain Anak-Anak
Abstrak: Play is a need for children to learn how to socialize and develop their creativities. Agricultural landscape of Desa Pangulah Utara has a potential that could be developed as a children playground facility. The open space can be exploited as a mean to support play activities. The purpose of this research is to create a management plan of agricultural landscape of Desa Pangulah Utara, Kecamatan Kota Baru, Kabupaten Karawang, Jawa Barat, as a means of children playing and helps children to learn how to socialize and interact with their environment, also to introduce more about Sundanese traditional games. Data that used in this research consists of physical, biophysical, social, and managerial data. These data were analyzed descriptively, statistically, and by means of SWOT method. The results of SWOT analysis are six recomendation of management strategies of Desa Pangulah Utara as a mean of children playground: (1) providing the facilities that supporting children to play especially to play Sundanese traditional games, (2) providing play areas, (3) making the playing program that can increase the interest of children about Sundanese traditional games, (4) using local vegetation as materials of Sundanese traditional games, (5) making landscape management plan of agricultural landscape of Desa Pangulah Utara as children playground, and (6) specifying the areas that used as childreen playground without changing its function.
Keyword: traditional games, SWOT analysis, playing, management, agricultural landscape

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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