Judul: Pertumbuhan Gmelina (Gmelina arborea Roxb) pada Beberapa Pola Agroforestri di Desa Sekarwangi, Kecamatan Malangbong, Kabupaten Garut
Abstrak: Agroforestry is a system of land use which combines wooden plants (trees, shrubs, rattan) with plants is not a wooden or may with grass (pasture), sometimes there is a component of livestock and other animals so that ecological interactions between plant formed a wooden and other components. Selection of inappropriate agroforestry pattern will affect the growth of plants by the existence of high competition to get nutrient elements, air, and light of the Sun. Agroforestry patterns which were selected as a treatment are agroforestry pattern one (AgF1) consists of gmelina, suren, mahoni, pete, the dominance of chili plants, and corn. Agroforestri pattern two (AgF2) consists of gmelina, sengon, dominance of plant corn, and cassava. Agroforestri pattern three (AgF3) consists of gmelina, suren, crop the dominance of cassava, peanut, and corn. Observed parameters is gmelina dimension, which are total tall, total diameter trunk, tall branch without crown, and the crown diameter. This research aims to analyze the effect of agroforestry pattern on the growth of G. arborea and analyze the relationship of land use management system with the growth of G. arborea. The research held in the Sekarwangi Village, Malangbong, Garut in December 2011 up to February 2012. This research uses a Complete random Design (RAL) with one factorial analysis, i.e. agroforestry pattern. The result showed that agroforestry pattern one give the best effect to gmelina growth. It obtained based on the test and test fingerprints variegated Duncan. Agroforestry pattern one gives highest average on all parameters. Besides that, land management system agroforestry pattern one also exert the best effect to the gmelina growth
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Judul: Panjang dan Kedalaman Akar Lateral Gmelina (Gmelina arborea Roxb.) pada Beberapa Pola Agroforestri di Desa Sekarwangi, Kecamatan Malangbong, Kabupaten Garut
Abstrak: Along with the growing population, the need for land was growing. Therefore, it takes an effort that can harness land optimally as agroforestri. Agroforestry is a system of land use by plants of forestry with crop and/or livestock. This system also has the value of economic, ecological, and social events. However, agroforestry also have negative interactions such as competition, nutrient elements, water, and light between forestry with crop plants. So do research on rooting on some patterns of gmelina (Gmelina arborea) agroforestry different. Gmelina is a fast-growing species of forestry. Growth character of gmelina relatively rapid can be used as a species for rehabilitation of land such as in DAS Cimanuk-Citanduy residing in the land belongs to the people. The purpose of this study is to assess the depth and length of the lateral roots gmelina, knowing the biophysical environment and data processing system of land on three different patterns in the location of research. The study is expected to be a reference for the selection of a suitable type of crop grown under the stands gmelina. Gmelina rooting was obtained by digging the root depth and depth variations have different length. These variations are due to the existence of biophysical properties of differences as well as the processing of land environment is different in each pattern. This measurement is done in the Census of every pattern. As for the crop that can be applied on the system agroforestry on site research is robusta, grapefruit, cardamom, turmeric, curcumae, pineapple, panili, orchid, deer horns, and honey bees
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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