Judul: Konsumsi Fast Food dan fakto-faktor yang berhubungan dengan kegemukan anak sekolah di SD Bina Insani Bogor:
Abstrak: Tujuan umum penelitian ini adalah untuk mempelajari konsumsifast food dan menganalisis faktor-faktor yang berhubungan dengan kegemukan anak sekolah di SD Bina Insani Bogor. Sedangkan tujuan khusus dari penelitian ini adalah (1) mengetahui prevalensi kegemukan anak sekolah di SD Bina Insani Bogor (2) mempelajari fiehensi konsumsi fast food anak sekolah gemuk dan normal (3) mengetahui tingkat konsumsi energi anak sekolah gemuk dan normal (4) mempelajari aktivitas fisik pada hari sekolah dan hari libur anak sekolah gemuk dan normal (5) menganalisis hubungan pendidikan ayah dan ibu, pengetahuan gizi ibu, pendapatan keluarga, besar keluarga, informasi pangan dan tingkat kesukaan dengan frekuensi konsumsi fast food anak sekolah di SD Bina Insani Bogor (6) menganalisis faktor-faktor yang berhubungan dengan kegemukan anak sekolah di SD Bina Insani Bogor. Desain penelitian ini adalah cross sectional study dengan menggunakan metode survey. Penelitian dilakukan selama 3 bulan yaitu pada bulan April-Juni 2007 dengan anak berjumlah 60 orang (30 anak gemuk dan 30 anak normal) yang diambil secara acak. Data dianalisis menggunakan Microsoft Excel 2003 dan SPSS 15.0 for Windows. Analisis statistik yang digunakan pada penelitian ini antara lain uji beda t (independent samples T-test), uji Mann Whitney, korelasi Spearman, dan korelasi Pearson.
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Judul: Pengetahuan Gizi, Aktivitas Fisik, Konsumsi Snack Dan Pangan Lainnya, Pada Murid Sd Bina Insani Bogor Yang Berstatus Gizi Normal Dan Gemuk
Abstrak: Childhood obesity is increases rapidly in developing country. It’s has important short-term and long-term medical consequences also psychosocial consequences. The objective of the research is to analyze nutritional knowledge, physical activity, snack habit, in normal and overweight of Bina Insani elementary students at Bogor. The cross sectional design was used in this study to elaborate nutritional knowledge, snack habit, and physical activity in normal and overweight students. The total number of 80 students sample was chosen randomly. It consists of 40 students sample with normal nutritional status and 40 students sample with overweight nutritional status. Primary data consisted of physical activity recall (1x24 hours), food consumption recall (2x24 hours), anthropometry data (weight and height), social-economy’s sample condition, food habit, and snack habit using Food Frequency Questionnaire (FFQ). Secondary data were included data of Bina Insani Elementary School. The result showed no relation between level education and wage of parent, nutritional knowledge level, nutritional attitude, snack habit and physical activity with nutritional status of sample. The average energy adequacy level according to WNPG (2004) respectively 82,2% in normal sample and 73,6% in overweight sample.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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