Judul: Aktivitas Antimikrob Kombinasi Ekstrak Daun Henna (Lawsonia inermis L.) dan Rifampisin terhadap Mycobacterium tuberculosis secara In Vitro
Abstrak: Lawsonia inermis L. known as henna in Indonesia is widely used as herbal medicinal plant to treat various diseases, including tuberculose. The objective of this research is to analyze combination of henna leaves extract activity against M. tuberculosis by evaluate the mininum inhibitory concentration (MIC). Dry leaves were macerated by using ethanol 96%. Then, the leaves went through filtration and evaporation process to gain concentrated extract. The extract was mixed with M. tuberculosis suspension and antibiotic solution at concentration of 10.000 ppm, 20.000 ppm, 40.000 ppm, 80.000 ppm and incubated 37oC. After two weeks the MIC of leaves extract and combination extract and antibiotic that inhibit the growth of M. tuberculosis was observed. The result showed MIC of henna leaves extract is 20.000 ppm, while MIC henna leaves extract and rifampicin 4.000 ppm less than 10.000 ppm. Combination of henna leaves extract and rifampisin treatment is more effective than extract treatment to kill M.tuberculosis.
Keyword: Tuberculosis, Ogawa media, MIC, M.tuberculose, Henna leaves

Judul: Aktivitas Anti-Mycobacterium dari Fraksi Metabolit Sekunder Bakteri Endofit Bacillus velezensis strain DJ4 Asal Jengkol (Archidendron pauciflorum)
Abstrak: Penemuan dan pengembangan obat anti-tuberkulosis (TB) baru yang efektif terhadap strain Mycobacterium yang resisten antibiotik perlu dilakukan untuk menurunkan angka kematian akibat TB. Mycobacterium smegmatis umum digunakan dalam penelitian awal penapisan senyawa anti-TB. Penelitian ini bertujuan memfraksinasi metabolit sekunder yang dihasilkan oleh bakteri endofit Bacillus velezensis strain DJ4 asal jengkol (Archidendron pauciflorum), menganalisis aktivitas anti-Mycobacterium-nya terhadap M. smegmatis, dan mengidentifikasi profil metabolit fraksi aktifnya. Lima puluh enam fraksi telah berhasil diperoleh melalui teknik kromatografi lapis tipis (KLT) dan kromatografi kolom. Sepuluh fraksi diantaranya memiliki aktivitas antibakteri terhadap M. smegmatis dengan minimum inhibitory concentration (MIC) berkisar antara 85–1325 μg/ml. Fraksi F53 merupakan fraksi paling potensial dengan nilai MIC terendah yaitu 85 μg/ml. Pada konsentrasi 1× MIC dan ½× MIC, fraksi tersebut mampu menghambat pembentukan biofilm M. smegmatis berturut-turut sebesar 68,3% dan 52,1%. Pengamatan menggunakan scanning electron microscope (SEM) menunjukkan bahwa matriks biofilm M. smegmatis yang diberi perlakuan fraksi F53 lebih tipis, kurang kompak, serta terpisah-pisah dalam beberapa bagian dibandingkan kontrol media maupun metanol. Analisis Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) menunjukkan bahwa fraksi tersebut mengandung lima jenis senyawa, antara lain 2-(p-anisyl)-5-methyl- 1-hexene, nigakilactone H, tetratriacontanamine, dan dua senyawa lainnya yang belum teridentifikasi.
Keyword: antimycobacterial, Archidendron pauciflorum, fractionation, MIC, Mycobacterium smegmatis

Judul: Implementation of Spatio-Temporal Change Query in Relational Database.
Abstrak: Geographic object changes in various views. Object changes are recorded using a model of static storage. No historical connection between old version data and new version data has been built. For that, a storage model of spatio-temporal queries that can analyze spatio-temporal changes has been developed. The object changes the geometry are grouped according to the taxonomy of Spatio-Temporal Pattern Change. This research has been able to apply the query patterns change including born, expand, merge, contract, split, normal and die. This query based on geometric (area) changes. The implementation of spatio-temporal change queries represents an object changes continuously with easy, change detection is done by comparing two versions of data. Keyword: spatio-temporal change query, spatio-temporal change pattern.
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