Judul: Identifikasi Respon Anatomi Daun dan Pertumbuhan Kenari, Akasia dan Kayu Manis terhadap Emisi Gas Kendaraan Bermotor
Abstrak: Pembangunan yang berkembang pesat dewasa ini, khususnya dalam bidana industri dan teknologi serta meningkatnya jumlah kendaraan bennotor yang menggunakan bahan bakar fosil (minyak) menyebabkan udara tereemar oleh gas-gas buangan yang merupakan sampingan dari proses pembakaran.
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Judul: Anatomi daun dan jumlah stomata dari beberapa jenis anakan tanaman peneduh di Balitro dan jalan tol Jagorawi
Abstrak: Kegiatan pembangunan transportasi yang semakin meningkat, di satu pihak memang memberikan manfaat yang besar bagi mobilitas penduduk, perekonomian masyarakat dan peningkatan kesejahteraan. Tapi di lain pihak, ternyata pembangunan transportasi juga akan dapat menimbulkan dampak negatif berupa pencemaran udara. Untuk mengatasi timbulnya gangguan akibat pencemaran udara di kota-kota besar, maka perlu dilakukan upaya penanggulangannya, antara lain dengan membangun hutan kota di kawasan-kawasan yang tercemar atau dengan cara penanaman pohon-pohon pinggir jalan secara merata dan terencana di seluruh kota. Penelitian ini bertujuan untuk memahami dan mempelajari bagaimana pengaruh dari pencemaran udara terutama yang disebabkan oleh kendaraan bermotor terhadap anatomi daun yang meliputi bentuk sel, panjang dan lebar sel serta jumlah stomata, dari anakan beberapa jenis tanaman peneduh, melalui pengamatan mikroskopis preparat awetan. Hasil penelitian diharapkan dapat membantu dalam pemilihan jenis pohon yang sesuai untuk ditanam di suatu daerah yang Tercemar yang disebabkan oleh kendaraan bermotor.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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