Judul: Efficacy of Aeromonas salmonicida Inactive Vaccine to Against Furunculosis on Juvenile Common Carp Cyprinus carpio
Abstrak: Penyakit furunkulosis adalah penyakit bakterial yang disebabkan oleh bakteri Aeromonas salmonicida. Penyakit ini menyerang komoditas budidaya ikan air tawar, salah satunya ikan mas. Vaksinasi dapat dijadikan salah satu solusi untuk mencegah penyebaran penyakit furunkulosis. Tujuan penelitian ini menguji efikasi vaksin inaktif Aeromonas salmonicida dengan dosis berbeda untuk mencegah penyakit furunkulosis dengan menganalisis respons imun spesifik dan non-spesifik pada benih ikan mas. Ikan uji yang digunakan dalam penelitian ini yaitu ikan mas dengan kisaran bobot 7,9±0,97 g dengan panjang 8,1±0,51 cm. Rancangan penelitian ini menggunakan rancangan acak lengkap dengan lima perlakuan dan tiga ulangan. Dosis vaksin yang digunakan yaitu V8 (108 CFU/mL), V7 (107 CFU/mL), dan V6 (106 CFU/mL). Parameter yang diamati yaitu total eritrosit, kadar hemoglobin, kadar hematokrit, total leukosit, aktivitas fagositik, dan titer antibodi. Vaksinasi dengan vaksin inaktif terbukti mampu meningkatkan respons imun non-spesifik dan spesifik pada ikan mas. Total eritrosit tertinggi pada perlakuan V8 sebesar 1,40±0,04(×106 sel/mm3). Kadar hemoglobin tertinggi pada perlakuan V8 sebesar 8,87±0,31 g%. Kadar hematokrit tertinggi pada perlakuan V8 sebesar 25,30±1,10%. Total leukosit tertinggi pada perlakuan V8 sebesar 5,20±0,30 (×104 sel/mm3). Aktivitas fagositik tertinggi pada perlakuan V8 sebesar 52,67±1,15%. Titer antibodi tertinggi diperoleh perlakuan V8. Dosis vaksin terbaik yaitu vaksin inaktif dengan dosis 108 CFU/mL dengan nilai RPS 71,79%., Furunculosis is a bacterial disease caused by the Aeromonas salmonicida bacteria. This disease attacks freshwater fish cultivation commodities, one of which is carp. Vaccination can be used as a solution to prevent the spread of furunculosis. The aim of this study was to test the efficacy of Aeromonas salmonicida inactivated vaccine with different doses to prevent furunculosis by analyzing the specific and non-specific immune responses of common carp. The test fish used in this study were carp with a weight range of 7,9 ± 0,97 g and a length of 8,1 ± 0,51 cm. The research design used a completely randomized design with five treatments and three replications. The vaccine dose is V8 (108 CFU/mL), V7 (107 CFU/mL), and V6 (106 CFU/mL). Parameters observed were total erythrocytes, hemoglobin levels, hematocrit levels, total leukocytes, phagocytic activity, and titer antibody. Vaccination with inactivated vaccines has been shown to increase non-specific and specific immune responses in common carp. The highest total erythrocyte was in the V8 treatment of 1,40 ± 0,04 (×106 cells/mm3). The highest hemoglobin level was in the V8 treatment of 8,87 ± 0.31 g%. The highest hematocrit level was in the V8 treatment of 25,30 ± 1,10%. The highest total leukocyte was in the V8 treatment of 5,20 ± 0,30 (×104 cells/mm3). The highest phagocytic activity was in the V8 treatment of 52,67 ± 1,15%. The highest titer antibody was obtained by the V8 treatment. The best vaccine dose is an inactivated vaccine with a dose of 108 CFU/mL with an RPS value of 71,79%.
Keyword: Aeromonas salmonicida, dosis, furunkulosis, imun, vaksinasi

Judul: Efektivitas vaksin DNA anti-KHV pada benih ikan mas Cyprinus carpio melalui metode perendaman dan perlakuan hiperosmotik
Abstrak: Common carp production has decreased since the mass deaths caused by disease of koi herpesvirus (KHV). Therefore, safe technique is needed and can be applied in bulk with cost efficient. The purpose of this research was verified influence of DNA vaccine anti-KHV with dose of 1,3 x 108 cfu / mL was performed through immersion in different duration after hyperosmotic treatment for 2 minutes treatment to produced the resistence common carp. There were six treatments with three replications, A: vaccination for 60 minutes immersion, B: 90 minutes, C: 120 minutes, positive control: fish were not vaccinated and infected with KHV, negative control: fish not vaccinated and were not infected with KHV, control bacteria: fishes that soaked with bacteria did not containing vaccine. The results showed that vaccination for 60 minutes giving highest relative survival (93.55%). Thus, vaccination by immersion and hyperosmotic treatment effectively improve the survival and relative percent survival of fish, and this method can be an alternative in the early control of KHV infection in common carp and koi.
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Judul: Penentuan Nilai Eigen dan Vektor Eigen dari Matriks Tridiagonal 2-Toeplitz dengan Pendekatan Polinomial Chebyshev
Abstrak: The eigenvalues and eigenvectors of a matrix can be determined by finding its characteristic polynomials. The characteristic polynomials of a tridiagonal 2-Toeplitz matrix is shown to be closely connected to polynomials which satisfy the Chebyshev recurrence relationship. If the order of the matrix is odd, then the eigenvalues are found explicitly in terms of the Chebyshev zeros and the eigenvectors are found in terms of the polynomials satisfying the recurrence relationship. For even ordered matrices, the situation is more complicated. The problem in these cases is that although the Chebyshev recurrence formula is still applied, its initial values are not generating Chebyshev polynomials
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