Judul: Perencanaan Lanskap Kawasan Permukiman Berbasis Mitigasi Penurunan Muka Tanah Studi Kasus Kecamatan Cengkareng Jakarta Barat
Abstrak: Kecamatan Cengkareng merupakan salah satu kecamatan yang terdapat di Kota Jakarta Barat yang kerap mengalami fenomena penurunan muka tanah. Penurunan muka tanah (land subsidence) merupakan suatu proses gerakan penurunan muka tanah yang didasarkan atas suatu datum tertentu (kerangka referensi geodesi) dimana terdapat berbagai macam variabel penyebabnya (Marfai 2006). Penurunan muka tanah dapat disebabkan oleh beberapa faktor, yaitu faktor alami, faktor pengambilan air tanah dan faktor massa bangunan. Penurunan muka tanah dapat berdampak, baik secara material maupun non-material. Untuk mengatasi dampak yang akan muncul dibutuhkan perencanaan tata ruang yang dapat meminimalisir dampak yang terjadi akibat penurunan muka tanah pada kawasan Kecamatan Cengkareng. Tujuan penelitian ini adalah menyusun rencana lanskap kawasan permukiman berbasis mitigasi bencana penurunan muka tanah. Penelitian dilakukan dengan pendekatan lanskap dalam proses perencanaan lanskap sebagaimana dikemukakan oleh Fabos (1979) yang terdiri tiga tahap, yaitu: penentuan sumber daya kritis, penentuan sumber daya bahaya dan penentuan kawasan permukiman yang dapat dikembangkan. Penelitian dilakukan untuk mengetahui karakteristik kawasan rawan bencana penurunan muka tanah, keterkaitan antara penurunan muka tanah dan bencana banjir, serta menghasilkan perencanaan tata ruang yang dapat menjadi solusi dari permasalahan penurunan muka tanah. Berdasarkan hasil analisis, rencana lanskap dituangkan kedalam empat zona yakni zona konservasi, zona rawan bencana, zona penyangga dan zona pengembangan. Setiap zona dikembangkan secara fungsional dan terintegrasi dengan kebutuhan ruang, fasilitas serta aktivitas masyarakat Kecamatan Cengkareng., Cengkareng District, located in West Jakarta City, experiences frequent land subsidence phenomenon. Land subsidence is a process of land subsidence movement based on a certain datum (geodesy reference framework) where there are various variables (Marfai 2006). This process is caused by natural, groundwater extraction, and building mass factors. This phenomenon could lead to material and non-material impacts. In order to minimize the negative effects of land subsidence in the area, there is a need for spatial planning. The aim of this research is to develop a landscape plan for residential areas based on land subsidence disaster mitigation. The process involved three stages of landscape planning as proposed by Fabos (1979) determining critical resources, determining hazardous resources and determining residential areas that can be developed. The research was conducted to identify areas prone to land subsidence disasters, the relationship between land subsidence and flood disasters, and to produce spatial planning that can mitigate the problem of land subsidence. Based on the analysis, the landscape plan is divided into four zones: conservation, disaster-prone, buffer and development zones. Each zone is developed with the needs of the community in mind, integrating space, facilities, and activities.
Keyword: Sumber Daya Bahaya, Sumber Daya Kritis, Sumber Daya Pengembangan, Penurunan Muka Tanah, Critical Resources, Development Resources, Hazardous Resources, Land Subsidence

Judul: Perencanaan Lanskap Pesisir Berbasis Mitigasi Bencana Banjir Rob di Kecamatan Penjaringan Jakarta Utara
Abstrak: Daerah Khusus Ibukota Jakarta merupakan salah satu kota di Indonesia dengan kepadatan penduduk yang tinggi dan memiliki laju pembangunan yang pesat. Kecamatan Penjaringan merupakan salah satu kawasan pesisir di Jakarta Utara yang merupakan daerah rentan terhadap bencana banjir, salah satunya yaitu banjir rob yang disebabkan naiknya permukaan air laut yang menyebabkan berbagai dampak kerugian bagi masyarakat. Alih fungsi lahan juga berdampak pada ekosistem kawasan pesisir perkotaan menjadi lebih tidak seimbang, sehingga dibutuhkan perencanaan lanskap berbasis mitigasi bencana banjir rob. Perencanaan dilakukan hasil analisis yang terdiri dari analisis kerentanan fisik lahan terhadap bencana banjir rob, analisis kesesuaian area evakuasi bencana, dan analisis kesesuaian area permukiman terhadap banjir rob. Konsep dasar perencanaan yang dikembangkan dari penelitian ini adalah mitigasi bencana banjir rob dengan penataan ruang di kawasan pesisir. Hasil penelitian ini berupa rencana lanskap yang terdiri dari rencana tata ruang, aktivitas, fasilitas dan sarana prasarana, sirkulasi, mitigasi dan vegetasi. Rencana tata ruang dibagi menjadi ruang konservasi, ruang hijau publik, ruang terbangun dan evakuasi. Rencana aktivitas, fasilitas dan prasarana untuk menunjang kebutuhan pada rencana tata ruang. Rencana sirkulasi dibedakan menjadi jalur primer, sekunder, dan tersier. Rencana sirkulasi menunjang jalur evakuasi bencana banjir rob. Rencana mitigasi dibagi menjadi mitigasi struktural dan non struktural. Rencana vegetasi dibagi menjadi vegetasi pelindung, peneduh, dan pengarah untuk menunjang estetika lanskap kawasan pesisir berdasarkan fungsinya.
Keyword: banjir rob, kerentanan, kesesuaian, mitigasi, perencanaan

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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