Judul: Pola Pemberian Makan dan Preferensi Makanan Tambahan Anak di Bawah Umur Dua Tahun di Desa dan Kota (Studi Kasus di Desa Caringin, Kabupaten Bogor dan Kelurahan Kebon Pedes, Kotamadya Bogor)
Abstrak: Penelitian ini bertujuan untuk mengetahui gambaran pola pemberian makan anak baduta dan faktor-faktor yang mempengaruhinya, serta preferensi makanan anak baduta, di desa dan di kota. Penelitian -dilaksanakan di desa Pasir Buncir , Kabupaten Bogor dan Kelurahan Kebon Pedes, Kotamadya Bogor, dari bulan Agustus sampai dengan bulan Desember 1991. Pengambilan contoh dilakukan secara acak, dengan jumlah contoh masing-masing di desa dan di kota yaitu 11 anak berumur 4-6 bulan, 25 anak berumur 7-12 bulan dan 24 anak berumur 13-24 bulan. Jadi seluruhnya ada 60 anak di desa dan 60 di kota.
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Judul: Studi Pola Pengasuhan Anak, Status Gizi dan Perkembangan Anak Badut di Desa Cibatok 2, Kecamatan Cibungbulang, Kabupaten Bogor
Abstrak: Tujuan dari penelitian ini adalah mengetahui pola asuh makan dan lingkungan anak baduta, mengetahui status gizi dan perkembangan anak baduta, mengetahui hubungn antara sosial ekomoni keluarga dengan pola asuh makan dengan status gizi anak baduta dan mempelajari hubungan status gizi dan pola asuh lingkungan anak dengan perkembangan anak baduta.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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