Judul: Persepsi dan partisipasi masyarakat desa sekitar hutan terhadap sistem PHBM di Perum Perhutani (Kasus di KPH Cianjur Perum Perhutani Unit III, Jawa Barat)
Abstrak: Hutan merupakan salah satu kekayaan Negara Indonesia yang dikelola oleh Negara untuk kesejahteraan masyarakat. Pengelolaan hutan dilakukan oleh salah satu Badan Umum Milik Negara (BUMN) yaitu Perum Perhutani KPH Cianjur yang mengelola hutan secara lestari untuk meningkatkan kesejahteraan masyarakat dengan sistem Pengelolaan Hutan Bersama Masyarakat (PHBM). Keberhasilan sistem PHBM ini dalam meningkatkan kesejahteraan masyarakat desa hutan dapat dilihat dari persepi dan partisipasi masyarakat terhadap kegiatan PHBM Perum Perhutani KPH Cianjur. Jika persepsi masyarakat semakin tinggi maka tingkat partisipasi masyarakat dalam melakukan kegiatan PHBM semakin tinggi , sehingga kegiatan PHBM akan berjalan sesuai rencana dan kesejaheraan masyarakat meningkat.
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Judul: Partisipasi Masyarakat Desa Hutan dalam Program Pengelolaan Hutan Bersama Masyarakat di KPH Cepu Perum Perhutani Unit I Jawa Tengah
Abstrak: Since a long time ago, the people of Indonesia in satisfying such needs as houses, clothing, food, medicine, and environmental services have depended heavily on forests. The increase of population has caused the living necessities to increase and triggered the exploitation of forest resources commercially at a large-scale. Based on the recapitulation of KPH Cepu, the incidence of theft and forest fires is high every year. Responding to the increasing forest disturbances, Perhutani applied several new multi-sector policies related to forest management. One form of the new policies is the PHBM (Forest Management together with Community) program. KPH Cepu began to launch the program in 2003. This program opens an opportunity for people to participate directly in forest management. This started with the establishment of cooperation between Perhutani and LMDH (Rural Forest Community Institution). One element of the successful implementation of PHBM is the effectiveness of LMDH. An effective LMDH is one that involve the community in each activity. The study was conducted in the villages of Bleboh and Nglebur, Perum Perhutani KPH Cepu Unit I Central Java, from September 2011 through November 2011. The data used were of primary and secondary types. The purpose of this study was to identify the activities of PHBM, analyze people‟s participation in the PHBM program, and analyze the effectiveness of LMDH. the number of selected villages was two, namely the village of Bleboh and Nglebur with 30 respondents in each village. Based on the research results, there are two types of activities in PHBM at KPH Cepu: the activities inside the forest area and the activities outside the forest area. The inside activities include planting, maintaining, intercropping, and security. The outside activities involve the establishment of saprotan shop, cattle and goats breeding, empon-empon farming, and nurseries. The implemented program of PHBM in LMDH of Wana Sumber Mulyo consists of the activities only inside forest area, whereas the PHBM program in LMDH of Wana Tani Makmur includes the activities both inside and outside the forest area. Participation of pesanggem in PHBM program is still partial, i.e. limited to the phase of implementation and allocation of shared profit from non-wood products. The effectiveness LMDH as an institution has not yet complied with the four principles of good forest governance.
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Judul: Influenza Specific Immunoglobulin Y Purification from Egg Yolk with Sodium Chloride Precipitation Method
Abstrak: Imunoglobulin Yolk (IgY) yang terdapat dalam kuning telur telah banyak dimanfaatkan untuk imunoterapi dan imunodiagnostik. Salah satu kendala dalam pemurnian IgY dari kuning telur adalah pemisahan Ig dengan lemak, sehingga teknik pemurnian Ig selalu berkembang. Penelitian ini bertujuan untuk melakukan pemurnian IgY dari kuning telur dengan metode pengendapan menggunakan sodium chloride (NaCl) serta melakukan karakterisasi IgY spesifik influenza. Sodium chloride dipilih karena bahan kimia ini relatif murah, ramah lingkungan, dan tidak merusak imunoaktivitas IgY. Kuning telur yang digunakan dalam penelitian ini berasal dari telur ayam layer yang divaksinasi dengan vaksin influenza Flubio® yang berisi virus Influenza A/H3N2, A/H1N1 dan B/Washington. Pemurnian IgY hanya dilakukan pada kuning telur yang mengandung antibodi spesifik influenza. Deteksi keberadaan antibodi spesifik influenza ditentukan dengan uji agar gel presipitasi (AGP) dan metode enzyme linked immunosorbent assay (ELISA). Konsentrasi IgY hasil pemurnian dihitung dengan spektrofotometer dan karakterisasi protein dilakukan dengan metode Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Konsentrasi protein dari hasil pemurnian IgY memiliki nilai rata – rata 50,403 mg/mL. Hasil SDS-PAGE menunjukkan enam pita protein, yaitu struktur IgY utuh (174,63 kDa), Apoprotein-LDL (129,77 kDa), rantai berat IgY (66,54 kDa), Fab ( 47,65 kDa), rantai ringan IgY (21,85 kDa), dan β-livetin (36,74 kDa). IgY hasil pemurnian masih dapat berikatan dengan antigen influenza berdasarkan uji ELISA. Hasil penelitian ini menunjukkan bahwa pemurnian IgY menggunakan metode pengendapan NaCl dapat memurnikan IgY dengan struktur utuh, meskipun masih ada dua protein tidak spesifik hasil pemurnian. IgY yang dapat berikatan dengan antigen influenza mengindikasikan prospek pemanfaatannya untuk pencegahan influenza dan imunodiagnostik., Yolk immunoglobulin (IgY) contained in egg yolk has been widely used for immunotherapy and immunodiagnostics. One of the obstacles in IgY purification from egg yolk is separating Ig from fat, so that IgY purification techniques are always developing. This study aimed to purify IgY from egg yolk using sodium chloride (NaCl) precipitation method and to characterize influenza-specific IgY. Sodium chloride was chosen because this chemical is relatively unexpensive, environmentally friendly, and does not impair IgY immunoactivity. The egg yolks used in this study were collected from layer eggs vaccinated with the Flubio® influenza vaccine, which contains the Influenza A/H3N2, A/H1N1 and B/Washington viruses. IgY purification was only carried out on egg yolks containing influenza specific antibodies. The presence of influenza-specific antibodies was determined by the agar gel precipitation (AGP) test and the enzyme linked immunosorbent assay (ELISA) method. The purified IgY concentration was determined using spectrophotometer, and protein characterization was performed using the Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) method. Protein concentration from purification has a mean value of 50,403 mg/mL . SDS-PAGE result showed six protein bands, namely intact IgY structure (174,63 kDa), Apoprotein-LDL (129,77 kDa), IgY heavy chain (66,54 kDa), Fab (47,65 kDa), IgY light chain (21,85 kDa), and β-livetin (36,74 kDa). The purified IgY still able to bind with influenza antigen based on the ELISA test. The results of this study indicate that purification of IgY using the NaCl precipitation method can purify IgY with intact structure, although there are still two unspecific proteins in the purification results. The resulting IgY can bind to influenza antigens indicating the
Keyword: Immunoglobulin Y, influenza, egg yolk, purification, sodium chloride