Judul: Introduksi dan Ekspresi Gen Hormon Pertumbuhan pada Ikan Mas Transgenik
Abstrak: Common carp Cyprinus carpio is one of the economically valuable aquaculture fish species in Indonesia. This research was aimed to produce transgenic common carp that grow rapidly by over-expressing the tilapia growth hormone (tiGH) gene. Trangenic common carp were produced by sperm-mediated gene transfer using electroporation method. In this study, two electroporation programs that developed by other researcher were applied to obtain a suitable electroporation method for common carp. The electroporated sperm were then used to fertilize eggs to produce F0 transgenic fish. Transgenic F0 carrying tiGH in their sperm were crossed with non-transgenic fish to produce transgenic first (F1) generation. Identification of transgenic fish was performed by PCR method using specific primer for tiGH gene. Expression of tiGH was analyzed using RT-PCR method and growth measurement at F1 fish generation. The results showed that electroporation of sperm using 50 Volt/cm, and DNA concentration of 25 ng/μl was produced higher percentarge of fish carrying tiGH in their gamet compared to that of another electroporation program (40 Volt/cm, DNA concentration of 50 ng/μl). Five sexually matured males F0 transgenic fish were selected and mated with non-transgenic to produce F1 generation. Percentage of F1 progenies carrying tiGH was 47,2-66,7%. Variated measure of F1 genereations was 10,1->12,0 cm size (22,5%-42,9%), 8,1->10,0 cm size (14,3%-42,9%), 6,1->8,0 cm size (11,9%-29,2%), 4,1->6,0 cm size (0,0%-39,3%). Increasing of growth of F1 was about 1,4-1,87 times for non transgenic fish. Survival of transgenic and non-transgenic F1 was generally similar. RT-PCR analysis showed that the F1 transgenic fish have tiGH gene expression in caudal fin, while no expression in non-transgenic fish were found. Thus, the increased body weight of F1 transgenic common carp was associated with expression of tiGH gene, and this fish can be used as broodstock for the production of fast-grow stable transgenic common carp.
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Judul: Performa Reproduksi Ikan Mas (Cyprinus carpio L) Jantan Transgenik Hormon Pertumbuhan Generasi Kedua
Abstrak: Fertility and viability are common parameters used to evaluate environmental safety as well as breeding potency of transgenic organisms. This research was performed to evaluate the reproductive performance of F2 generation GH (growth hormone) transgenic male common carp. This research used completely random design with two factors (transgenic and non-transgenic) and three replications. Three GH transgenic male common carp (TGJ) and three non-transgenic (NTJ) fish were crossbred with two non-transgenic female common carps (NTB), resulting 12 families. Parameters observed were semen volume, motility, duration of motility, number of sperm cells, survival of embryo, hatching rate, survival of larva until 3 days old, and morphology of sperm and larva. The results showed that semen volume of TGJ and survival of juvenile in 30 days was higher compared to NTJ (p<0,05), while motility rate, duration of motility, number of sperm cells, survival of embryo, hatching rate, and survival of larva were similar (p>0,05). Furthermore, there were no abnormalities found in sperm morphology of TGJ and the hatched larva of TGJ x NTB crossbred were the same with NT. As the conclusion, reproductive performance of GH transgenic male common carp showed good and similar result with the non-transgenic fish.
Keyword: reproductive performance, male broodstock, tiGH gene, common carp

Judul: Konsep Pola Permukiman Suku Baduy Dalam di Desa Kanekes, Kabupaten Lebak, Banten.
Abstrak: Suku Baduy yang bermukim di Desa Kanekes merupakan salah satu masyarakat yang memegang erat adat Sunda. Desa Kanekes terletak pada wilayah Kecamatan Leuwidamar, Kabupaten Lebak, Banten. Terdapat tiga desa utama dalam Desa Kanekes, yakni Kampung Cikeusik, Cikartawarna, dan Cibeo. Masyarakat Baduy berpegang teguh terhadap adat leluhur sehingga membentuk suatu kelompok yang terisolasi dari dunia luar. Kearifan lokal yang dimiliki oleh Suku Baduy terhadap alam, kesederhanaan, dan toleransi akan lingkungan sekitar mempengaruhi terbentuknya suatu budaya dari masyarakat baduy itu sendiri. Tatanan ruang Suku Baduy terbentuk karena adanya budaya yang mempengaruhinya sehingga membentuk suatu pola permukiman dengan aturan yang dipegang teguh. Penelitian ini dilakukan dengan menggunakan metode deskriptif melalui pengambilan informasi terkait budaya dan sejarah dari dokumen adat atau naskah adat dan wawancara melalui tokoh adat atau keyperson dan melakukan verifikasi melalui pengamatan langsung dengan observasi tapak. Penelitian ini mampu mengidentifikasi elemenelemen pembentuk lanskap permukiman tradisional Baduy, yaitu bangunan (rumah puun, rumah jaro, rumah warga, bale, bale panjang, leuit, dan saung lesung), ruang terbuka (alun-alun, leuweung lembur, huma, reuma, dan leuweung kolot), aksesibilitas (jalan utama), air (sungai dan pancuran), serta vegetasi (leuweung lembur, huma, reuma, dan leuweung kolot). Pola permukiman pada ketiga kampung di Baduy Dalam memiliki tata letak yang sama, yaitu berbentuk asimetris dengan arah utara dan selatan sebagai axis.
Keyword: DesaKanekes, pola permukiman, SukuBaduy, tata ruang