Judul: Pengetahuan Gizi, Keluhan Kesehatan, Kondisi Psikologis, dan Pola Pemberian Air Susu Ibu (ASI) Ibu Postpartum
Abstrak: Postpartum period is restoration period after childbirth with one of characteristics is the process of breastfeeding. This study was aimed to assess of nutritional knowledge, health complaints, psychological conditions, and their association with breastfeeding pattern on postpartum mother. A cross sectional study design and interview method was conducted in five rural areas in Dramaga Subdistrict, Bogor District, during the period of April to May 2013. Sample included in this study were 30 postpartum mothers who met inclusion criteria sample is the age of a baby 10−40 days and baby was not a first childbirth, she will be the respondent through the informed consent agreement, and be able communicate. The mayority postpartum mother had level of nutritional knowledge (60%) and health complaints (66.7%) were classified in low, and they have depression (56.7%). The nutritional knowledge of exclusive breastfeeding tends to correlate (r=0.126, p=0.508) exclusive breastfeeding practice. Meanwhile, lower health complaint tends to correlate with better breastfeeding practices.
Keyword: postpartum mother, breastfeeding, psychological, health complaints, Nutritional knowledge

Judul: Nutritional Status, Breastfeeding Knowledge, and Breastfeeding Practice in Cirebon Regency
Abstrak: Penelitian ini bertujuan untuk menganalisis hubungan status gizi ibu (asupan zat gizi dan indeks massa tubuh) dan pengetahuan ASI dengan praktik menyusui bayi usia 0–6 bulan di Kabupaten Cirebon. Desain penelitian ini adalah cross sectional study dengan jumlah subjek sebanyak 44 orang. Data yang digunakan merupakan data sekunder. Penelitian berlokasi di tiga desa di Kecamatan Gegesik, Kabupaten Cirebon yang merupakan lokus stunting. Sebanyak 47,7% subjek memiliki status gizi normal dan 29,5% subjek memiliki status gizi obesitas. Sebagian besar subjek memiliki tingkat kecukupan energi dan zat gizi yang belum mencapai kebutuhan harian. Sebanyak 63,6% subjek memiliki pengetahuan tentang ASI yang baik dan 43,2% subjek memiliki praktik menyusui yang kurang sesuai. Tidak terdapat hubungan signifikan antara tingkat kecukupan energi dan zat gizi ibu dengan status gizi bayi (p>0,05). Tidak terdapat hubungan signifikan antara karakteristik, status gizi, dan pengetahuan ibu mengenai pemberian ASI dengan praktik menyusui (p>0,05)., The purpose of this study is to analyze the relationship between maternal nutritional status (nutrient intake and body mass index) and breastfeeding knowledge with breastfeeding practices for infants aged 0-6 months in Cirebon Regency. The design of this research is a cross sectional study with a total of 44 subjects. The data used is secondary data. The study is located in three villages in Gegesik District, Cirebon Regency which were the locus of stunting. The amount of 47.7% subjects have normal nutritional status and 29.5% of subjects have obese nutritional status. Most of the subjects had adequate levels of energy and nutrients that had not yet reached their daily needs. The amount of 63.6% subjects have good knowledge of breastfeeding and 43.2% of the subjects have inappropriate breastfeeding. There is no significant relationship between the level of energy and nutritional adequacy of the mother and the nutritional status of the infants (p>0.05). There is no significant relationship between maternal characteristics, nutritional status, and knowledge of breastfeeding with breastfeeding practices (p>0.05).
Keyword: breastfeeding, breastfeeding knowledge, breastfeeding practice, nutritional adequacy, nutritional status

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
Keyword: