Judul: Sikap Masyarakat Kelurahan Pancoran Mas Terhadap Taman Hutan Raya Pancoran Mas, Depok.
Abstrak: Pancoran Mas Grand Forest Park is Located among residences, resulting interaction between grand forest park and the community. Today, only with a very bad repair iron fence that surrounding the park. This site had been neglected for several yaers. The main problem now is the management of this site. The effectivenes of grand foret park management depend on the recognition and support of the community living around the park. Knoweledge, attitudes and behaviour of the people will affect the grand forest park existence and conditions. Attitudes is a component that forms behaviour. This research is intended to measure the attitude of the people towards Pancoran Mas Grand Forest Park
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Judul: Community Interaction and Potential of Useful Plants in Pancoran Mas Grand Forest Park, Depok.
Abstrak: Taman Hutan Raya (Tahura) Pancoran Mas pada awalnya merupakan kawasan Cagar Alam yang telah ada sejak ±300 tahun yang lalu dan merupakan CA pertama di Indonesia. Meskipun keberadaannya sangat penting dalam sejarah perkembangan kawasan konservasi di Indonesia namun perhatian baik dari pihak pengelola maupun masyarakat di sekitarnya masih terbilang rendah. Penelitian ini bertujuan untuk mengidentifikasi interaksi masyarakat terhadap Tahura Pancoran Mas, komposisi vegetasi dan potensi spesies tumbuhan berguna yang terdapat di Tahura Pancoran Mas. Penelitian ini diharapkan dapat menjadi acuan pengelola dalam pengembangan kawasan serta menjadi informasi bagi masyarakat sekitar Tahura. Metode yang digunakan dalam penelitian ini terdiri dari wawancara dan analisis vegetasi. Pemilihan responden dalam wawancara menggunakan metode purposive sampling dengan jumlah responden sebanyak 30 orang. Analisis vegetasi dilakukan dengan metode jalur berpetak dengan total luasan petak contoh sebesar 2 ha. Hasil penelitian menunjukkan sebanyak 83,34% responden mengetahui spesies tumbuhan yang ada di sekitar pinggir Tahura namun hanya 36,7% responden pernah memanfaatkan tumbuhan. Berdasarkan hasil analisis vegetasi teridentifikasi 83 spesies dan 43 famili di Tahura Pancoran Mas dengan komposisi vegetasi pada tingkat pohon terdapat 27 spesies, tiang 23 spesies, pancang 12 spesies, semai 12 spesies, tumbuhan bawah 30 spesies, dan liana 4 spesies. Indeks Nilai Penting (INP) tertinggi pada tingkat pertumbuhan pohon, tiang, pancang, dan semai masing-masing dimiliki oleh spesies Arthocarpus elastica (56,52%), Macaranga rhizinoides (48,86%), Grewia acuminata (31,52%), dan Melicope lunuankeda (56,58%). Tumbuhan bawah didominasi oleh spesies Dioscorea aculeata (19,43%) dan liana didominasi spesies Spatholobus littoralis (93.54%). Keberadaan spesies tumbuhan dari marga Macaranga dan Mallotus mengindikasikan bahwa habitat di dalam kawasan sudah terganggu. Secara keseluruhan teridentifikasi 67 spesies dan 39 famili tumbuhan berguna dengan kelompok kegunaan tumbuhan obat mendominansi yaitu sebanyak 43 spesies. Kesimpulan dari penelitian ini adalah interaksi masyarakat dengan tumbuhan di dalam kawasan masih rendah dan terdapat potensi tumbuhan berguna di dalam kawasan yang dapat dimanfaatkan masyarakat sekitar. Saran yang dapat diberikan antara lain adalah pengenalan fungsi dan manfaat kawasan, pengelolaan yang lebih intensif, serta pengembangan kawasan Tahura Pancoran Mas agar keberadaannya tetap terjaga.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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