Judul: Purifikasi dan karakterisasi imunoglobulin Y (lgY) kuning telur ayam spesifik Streptococcus mutans menggunakan metode sodium dodecyl sulphate-poly acrilamide gel electrophoresis (SDS-page)
Abstrak: Penelitian ini bertujuan untuk mengetahui karakteristik lg Y anti Streptococcus mutans dari kuning telur yang terbentuk dengan menggunakan metode Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis (SDS-PAGE). Purifikasi lgY anti S.mutans menggunakan metode ekstraksi lg Y (metode Polson) dari kuning telur dan dilanjutkan teknik kromatografi (Fraction protein Liquid Chromatography / FPLC), sedangkan berat molekul (BM) lg Y diukur dengan metode Sodium Dodecyl Sulphate Poly Acrylamide Gel Electrophoresis (SDS-PAGE).
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Judul: Produksi imunoglobulin Y(Lg-Y) anti Streptococcus mutans pada serum dan kuning telur ayam single comb brown leghorn
Abstrak: Imunoglobulin Y merupakan antibodi yang terkandung dalam serum dan kemudian ditransferkan ke dalam telur ayam sebagai antibodi maternal. Imunoglobulin Y (Ig-Y) dapat dimanfaatkan penggunaannya sebagai imunoterapi dan imunodiagnostik. Imunoterapi yang biasa dilakukan adalah dengan pemberian imun pasiv secara oral. Dengan pemberian imun pasiv diharapkan tubuh host dapat membentuk kekebalan terhadap antigen spesifik, seperti Streptococcus mutans, bakteri penyebab plak dan karies gigi. Pengamatan produksi antibodi anti S. mutans dilakukan pada serum dan kuning telur dari ayam Single Comb Brown Leghorn. Sepuluh ayam Single Comb Brown Leghorn, umur 24 minggu, divaksinasi antigen utuh S. mutans yang telah diinaktivkan pada suhu 52° C selama 1 jam. Rute penyuntikan melalui intramuskular dan intravena. Pengujian keberadaan antibodi anti Streptococcus mutans ini dapat dilakukan dengan Metode Agar Gel Precipitation Test (AGPT). Pengujian dilakukan pada serum, kuning telur segar yang diencerkan dengan PBS, dan kuning telur yang diekstraksi dengan metode Water Dilution. Pengujian AGPT terhadap respon kekebalan primer dalam serum menunjukkan hasil positif pada 1 minggu setelah vaksinasi terakhir. Sedangkan uji AGPT pada kuning telur, pada waktu yang sama menunjukkan hasil negatif. Pada respon kekebalan sekunder pengujian AGPT serum, kuning telur segar yang diencerkan dan kuning telur yang diekstrak, memperlihatkan hasil positif. Hasil penelitian ini menunjukkan bahwa pada serum dan kuning telur dari ayam yang divaksinasi antigen Streptococcus mutans dapat memproduksi antibodi anti S. mutans dan telur ayam dapat berpeluang sebagai sumber produksi imunoglobulin Y (Ig-Y).
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Judul: Thermal Characteristics and Dynamics of Tropical Cyclone over Western North Pacific Ocean: A Case Study of Typhoon Songda (Chedeng) 2011
Abstrak: The frequent occasion of Tropical Cyclone (TC) over Western North Pacific (WNP) Ocean lies from 10˚N to 26˚N and 121˚E to 170˚E. Sources of TC development come from warm ocean temperature where latent heat is released during condensation processes and subsequently concentrated in the boundary layer. Relative humidity at 850-700 hPa is responsible to produce the most intense storm. Meanwhile, weak vertical wind shear between 850-200 hPa is one of the dynamical factors that lead to the development of TC. Pressure level of 850-200 hPa is chosen to minimize the thermodynamic effect in the lower troposphere. Typhoon Songda (Chedeng in the Philippines) or TSC lasted from May 18th to 30th, 2011 and strengthened into powerful Super Typhoon stage between May 24th and 27th. Long curving track and the exhibition of all sequences of TC life cycle, TSC is then considered as an ideal case of TC. The objective of the study with case of TSC is to investigate the role of thermal characteristics and dynamics on TC intensity. The research is carried out by using Advanced Research of Weather Research and Forecasting model (WRF ARW model) version 3.3. Two domains are employed in the model. The first domain situate from 02˚N to 40˚N and 118˚E to 149˚E and cover spatial resolution () of 33.1 km. The second domain situate from 09˚N to 27˚N and 119˚E to 132˚E with of 11 km. Timestep of the model () is 120 seconds. The model is supported by the following physical parameterization, i.e., Yonsei University Scheme (YSU) in the boundary layer, Kain-Fritsch scheme (KF) in the cumulus, and WRF Single-Moment 3-class scheme in the microphysics. Initial meteorological condition prior to the formation of the cyclone is generated using WRF ARW standard-release model. Sensitivity of the model output is obtained from a selected single-parameter change scenarios, namely, 10 % to 20 % decrease and increase of wind shear initial data at 850-200 hPa, and by 10 % to 20 % decrease of relative humidity initial data at 850-700 hPa. The result showed that the negative correlations between wind shear and intensity are found in wind shear scenarios. Wind shears of 10 ms-1 which lead to strengthening of the wind intensity in the early period of Typhoon development are found in wind shear scenarios. The effect of wind shear changes on wind intensity is statistically significant in the 20 % wind shear scenarios. During mature stage, weak wind intensity occurred when wind shear increase as given in the WRF standard-release and the 20 % decreasing scenario. Different situation is found in the 20 % increasing scenario where wind shear tend to weak and lead to strong wind intensity at this stage. Meanwhile, the reduction of moisture supply at 850-700 hPa resulting from 20 % decreasing relative humidity play a major role in the weakening of wind intensity and reduces mechanical energy of the cycle process as much as 300 J kg-1.
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