Judul: Pengarub Kombinasi Dosis Pupuk Kompos dan Pupuk Anorganik terhadap Pertumbuhan Bibit kakao, nilam, dan jarak
Abstrak: Percobaan dilakukan untuk mengetahui pengaruh kombinasi dosis pupuk kompos dan pupuk anorganik terhadap pertumbuhan bibit tanaman kakao dan jarak serta pertumbuhan dan produksi tanaman nilam. Percobaan diIaksanakan di Kebun Percobaan Cikabayan IPB Darmaga Bogor. Pada bulan januari·luni 2006. Ketinggian tempat percobaan 250 m dpl dengan jenis tanah Latosol Darmaga
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Judul: Pengaruh kombinasi pupuk organik cair dan pupuk anorganik terhadap pertumbuhan bibit kakao ( Theobroma cacao L. )
Abstrak: Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi Pupuk Organic Cair Bioton dan pupuk anorganik terhadap pertumbuhan bibit kakao Theobroma cacao L.). Penelitian ini dilaksanakan pada bulan Februari September 2003, bertempat di Kebun Percobaan Fakultas Pertanian IPB Pusat, Cikabayan Darmaga Bogor. Dalam percobaan ini digunakan Rancangan Acak Lengkap (RAL) dengan faktor tunggal enam perlakuan. Perlakuan terdiri atas 100% Pupuk Anorganik (PA)(B1), 80% PA + 2 ml Bioton / 1 air (B2), 60% PA + 4 ml Bioton /1 air (B3), 40% PA + 6 ml Bioton/1 air (B4), 20% PA + 8 ml Bioton/1 air (Bs), 0% PA + 10 ml Bioton / 1 air (B6). 100% PA adalah Urea 2 g (umur bibit 1 bulan) 4 g (umur bibit 25 bulan), SP-36 1 g dan KCl 2 g (umur bibit 1-5 bulan). Penggunaan berbagai kombinasi pupuk organik cair (POC) Bioton dan pupuk anorganik hanya berpengaruh sangat nyata terhadap tinggi tanaman mulai umur 16 MSPP dan luas daun total pada umur 22 MSPP, sedangkan untuk diameter batang, jumlah daun, bobot kering akar dan tajuk akibat kombinasi POC Bioton dan pupuk anorganik tidak berpengaruh nyata. Rata-rata tinggi tanaman mulai umur 16 MSPP hingga 20 MSPP menunjukkan pola di mana tanaman yang diberi 100% POC Bioton (B6) sangat nyata lebih tinggi daripada tanaman yang diberi 100% pupuk anorganik (B₁). Rata-rata tinggi tanaman yang diberi 100% POC Bioton (B6) dari umur 16 MSPP hingga 20 MSPP berturut-turut adalah 25.61 cm, 27.92 cm, 31.56 cm atau 19.84%, 28.25%, dan 39.70% lebih tinggi daripada rata-rata tinggi tanaman yang diberi 100% pupuk anorganik (B₁) Pada peubah luas daun total umur 22 MSPP, rata-rata luas daun total tanaman yang diberi 100% POC Bioton (B6) sangat nyata lebih tinggi daripada ..dst
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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