Judul: Dinamika Kandungan Bahan Organik (BOD) di Kalimalang Bekasi
Abstrak: Kalimalang is downstream such as from the West Tarum Canal, crosses Karawang, Bekasi, and DKI Jakarta (East Jakarta) regional. Kalimalang has suffered from pollution, especially the organic pollution upstream. This study aims to determine the dynamic of organic matter (BOD) each season, and its related the supporting parameters such as temperature, DO, TSS, pH, coliform, and debit water. In addition, STORET index is also used to determine the status of water quality. Organic matter highest in the dry season with a BOD value of 15.6 mg / l and lowest in rainy season with a BOD value of 4.89 mg / l. The high BOD value indicates that the waters are polluted It is also supported by STORET index value that is worth more than -21, which means that these waters are heavily polluted so it is not suitable for class status B.
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Judul: Kandungan Bahan Organik (BOD) Di Sungai Cipining Kabupaten Sumedang, Jawa Barat
Abstrak: Penelitian mengenai Kandungan Bahan Organik (BOD) Di Sungai Cipining Kabupaten Sumedang, Jawa Barat ini dilakukan pada tanggal 3 - 8 Juni 2003. Penelitian ini bertujuan untuk mengetahui kandungan bahan organik pada musim kemarau dengan pengukuran BOD, serta pengukuran parameter fisika dan kimia yang menjadi pendukung dalam menganalisa kandungan bahan organik di sungai tersebut. Selain itu juga nilai parameter tersebut dibandingkan dengan nilai baku mutu air kelas 2 berdasarkan PPRI No. 82 tahun 2001. Berdasarkan hasil pengamatan didapatkan kondisi perairan Sungai Cipining dengan parameter suhu, DO, pH, amonia, nitrit, nitrat, BOD, dan TSS berkisar antara: 22-24 °C; 6-8 mg/L; 7-8; 0,024 -0.063 mg/L; 0,197 -0,357 mg/L; 0,005-0,041 mg/L; 0.740-3,910 mg/L; 187-270 mg/L. Menurut baku mutu air kelas 2 PPRI No.82 tahun 2001 nilai parameter fisika dan kimia yang di ukur masing-masing suhu, DO, pH, amonia, nitrat, perairan memiliki nilai yang masih berada pada kisaran nilai baku mutu kelas 2. Hanya nitrit yang memiliki nilai diatas nilai baku mutu. Hal ini diduga karena proses dekomposisi amaonia menjadi nitrat baru berjalan pada proses perombakan amonia menjadi nitrit. Sedangkan Nilai BOD dan TSS berdasarkan hasil pengamatan masih berada pada kisaran yang baik untuk kegiatan perikanan. Dari hasil penelitian dapat disimpulkan bahwa kandungan bahan organik Sungai Cipining masih dapat dikatakan dalam kondisi baik dan masih dapat dilakukan aktivitas perikanan di DAS sungai tersebut.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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