Judul: Dampak Pelaksanaan Sistem Bapak Angkat terhadap Masyarakat Pengrajin Masyarakat Pengrajin Rotan (Studi Kasus Desa Cangkuang Galih, Kecamatan Curug, Kabupaten Tangerang, Propinsi Jawa Barat)
Abstrak: Tujuan penulisan praktek lapang ini adalah menelaah sejauh mana dampak yang ditimbulkan atas pelaksanaan sistem Bapak Angkat terhadap masyarakat pengrajin rotan, yakni bagi peningkatan kemampuan usaha industri kecil rotan, perluasan kesempatan kerja, perluasan kesempatan ekonomi, dan migrasi penduduk.
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Judul: Studi Kasus Industri Rotan Desa Tegalwangi, Kecamatan W eru Kabupaten Cirebon, Jawa Barat
Abstrak: Rotan merupakan salah satu komoditi ekspor non migas yang bersifat padat karya dan mempunyai prospek yang cerah dalam memperoleh devisa negara. Pesatnya permintaan terhadap produksi rotan telah menyebabkan liubungan kerjasama sub-kontrak dalam industri rotan tumbuh dengan baik antara pengusaha besar dengan pengusaha menengah, pengusaha kecil dan pengusaha rumahtangga. Hubungan sub-kontrak ini memiliki peranan penting, karena disamping dapat meningkatkan nilai tambah juga dapat menciptakan kesempatan kerja baru dan meningkatkan kesejahteraan pengrajin rotan.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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