Judul: Optimization of Extraction for Total Phenolics and Highest  Antioxidant Capacity in Gandarusa Using Two-Level Half Factorial Design
Abstrak: Gandarusa (Justicia gendarussa Burm. f.) telah digunakan sebagai obat tradisional dan dilaporkan memiliki senyawa fitokimia dan aktivitas antioksidan yang tinggi. Penelitian ini bertujuan untuk mengukur pengaruh perbedaan kondisi ekstraksi berdasarkan rasio cairan terhadap padatan, suhu, konsentrasi pelarut, waktu, dan pH tanaman gandarusa dengan two level half factorial design terhadap produksi metabolit sekunder dan kapasitas antioksidannya. Sampel diekstraksi sesuai dengan desain two level half factorial. Kandungan fenolik, kapasitas antioksidan DPPH dan FRAP tertinggi didapat oleh run-13 berturut-turut sebesar 28,15 mg GAE/g, 54,19 μmol TE/g, dan 218,73 μmol TE/g. Desain two level factorial digunakan untuk menentukan faktor yang signifikan terhadap ekstraksi kadar fenolik total, serta kapasitas antioksidan DPPH dan FRAP. Faktor suhu berperan signifikan (< 0,05) terhadap kadar fenolik total. Sedangkan pada kapasitas antioksidan DPPH didapatkan bahwa faktor cairan terhadap padatan dan pH berperan signifikan (< 0,05). Pada FRAP didapatkan bahwa faktor cairan terhadap padatan menjadi faktor yang berperan signifikan (< 0,05). Kata kunci: antioksidan, factorial, gandarusa, cairan terhadap padatan, suhu, Gandarusa (Justicia gendarussa Burm. f.) is often used as a traditional medicine and is reported to contain high levels of phytochemical compounds and antioxidant activity. This study aimed to measure the influence of different extraction conditions based on liquid to solid ratio, temperature, solvent concentration, time, and pH of gandarusa plant on the production of secondary metabolites and antioxidant capacity using a two-level half factorial design. Samples were extracted according to the two-level half factorial design. The highest phenolic content, DPPH and FRAP antioxidant capacity were obtained from run 13, with values of 22.75 mg GAE/g, 43.36 μmol TE/g, and 166.72 μmol TE/g, respectively. A two-level factorial design was used to determine the significant factors affecting the extraction of total phenolic content, DPPH antioxidant capacity, and FRAP antioxidant capacity. Temperature was found to have a significant effect (<0.05) on the total phenolic content. Meanwhile, for DPPH antioxidant capacity, the liquid to solid ratio and pH was found to be the significant factor (<0.05). In FRAP, it was found that the liquid to solid factor was a significant factor (<0.05). Keyword: antioxidant, factorial, gandarusa, liquid to solid, temperature
Keyword: antioxidant, factorial, gandarusa, liquid to solid, temperature

Judul: Optimization of Extraction of Phenolic Content and  Antioxidant Activity of Gandarusa (Justicia gendarussa Burm f.) Leaf with  Response Surface Methodology (RSM)
Abstrak: Ektraksi daun gandarusa (Justicia gendarussa Burm f.) dapat dipengaruhi oleh pelarut, suhu, dan waktu ekstraksi. Penelitian ini bertujuan menentukan suhu, waktu, dan konsentrasi pelarut optimum kadar total fenolik dan aktivitas antioksidan ekstrak etanol daun gandarusa dengan metode Respon Surface Methodology (RSM). Parameter uji yang dilakukan terhadap ekstrak daun gandarusa antara lain penentuan kadar total fenolik dengan metode Folin-Ciocalteu serta aktivitas antioksidan DPPH dan FRAP. Variabel bebas yang digunakan dalam desain eksperimen adalah konsentrasi etanol (10%, 50%, dan 90%), suhu (30℃, 45℃, dan 60℃) dan waktu ekstraksi (60, 120, dan 180 menit). Kadar total fenolik menunjukan korelasi yang positif dan signifikan terhadap aktivitas antioksidan FRAP daun gandarusa. Kondisi optimal untuk kadar total fenolik dan aktivitas antioksidan DPPH dan FRAP ekstrak daun gandarusa yaitu pelarut etanol 16,8%; suhu 30℃; dan waktu 180 menit. Nilai prediksi dan nilai verifikasi kadar total fenolik dan aktivitas antioksidan DPPH tidak berbeda secara signifikan., Extraction of gandarusa leaves (Justicia gendarussa Burm f. ) can be affected by solvent, temperature, and extraction time. This study aimed to determine the optimum temperature, time, and concentration of solvents, the total phenolic contents and antioxidant activity of gandarusa leaf ethanol extract by the Response Surface Methodology (RSM). Parameters of the test carried out on the extract of gandarusa leaf include determination of total phenolic contents using the Folin-Ciocalteu method as well as the antioxidant activity of DPPH and FRAP. The independent variables used in the experimental design were ethanol concentration (10%, 50%, and 90%), temperature (30℃, 45℃, and 60℃) and extraction time (60, 120, and 180 minutes). Total phenolic content showed a positive and significant correlation to the antioxidant activity of FRAP gandarusa leaves. Optimal conditions for total phenolic content and antioxidant activity of DPPH and FRAP of gandarusa leaf extract are 16,8% ethanol solvent; temperature 30℃; and time 180 minutes. The predictive value and verification value of the total phenolic content and antioxidant activity of DPPH did not different significantly.
Keyword: Antioxidant activity, gendarussa, independent variables, phenolic, RSM

Judul: Posisi Filogenetik Kura-Kura Carettochelys Insculpta  (Reptillia: Testudinata)
Abstrak: Carettochelys insculpta is an endemic freshwater turtle of southern Papua and northern Australia. Phylogenetic position of this turtle is remain unresolved until now. Most of the research conducted show close relationships of C. insculpta and members of Trionychidae family. However, some of the research show distance between C. insculpta and members of Trionychidae family. This research is conducted to analyze phylogenetic relationship between C. insculpta and other members of Testudinata using 12S rRNA, 16S rRNA, and both gene as markers. DNA samples were obtained from three different organisms of C. insculpta. Two species used as outgroups were Alligator mississippiensis and Iguana iguana. Phylogenetic analysis was carried out using Neighbor-Joining method with Felsenstein 1981 (F81) modeltest. A polytomy tree of Testudinata was produced by using 12S rRNA gene. However, analysis using 16S rRNA and both genes as markers confirmed that C. insculpta was located between the Chelonidae and the Trionychidae family.
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