Judul: Kasus Pada Pedagang Sayur di Kelurahan Tegal Lega, Kotamadya Bogor, Propinsi Jawa Barat
Abstrak: Pcnelitian ini menelaah pola migrasi dan kepemimpinan informal yang berlangsung pada kelompok migran pedagang sayur di Kelurahan Tegal Lega, Bogor. Kelompok pedagang sayur Tegal Lega ini merupakan sebuah kelompok yang cukup menarik karena: pertama, kelompok tersebut tinggal berkelompok dalam suatu wilayah (di daerah Ciwaluya,Tegal Lega); kedua, kelompok pedagang sayur itu memiliki pekerjaan yang sama sebagai pedagang sayur keliling; ketiga, kelompok migran berasal dari pedesaan yang mengalami proses migrasi berantai.
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Judul: Strategi Hidup Pedagang Sayur yang Bekerja di Sektor Informal (Kasus pada Pedagang Sayur di Kelurahan Tegal Lega, Kecamatan Bogor Tengah, Kotamadya Bogor, Jawa Barat)
Abstrak: Sejalan dengan perubahan dengan struktur perekonomian dari sektor pertanian ke non pertanian menyebabkan jumlah tenaga kerja yang bekerja di sektor pertanian menurun. Pergeseran tenaga kerja dari sektor pertanian ke non pertanian tidak secara otomatis mengubah mata pencaharian tenaga kerja di sektor pertanian. Keterbatasan kesempatan kerja sektor pertanian karena keterbatasan lahan mengakibatkan kelebihan tenaga kerja yang secara dinamis masuk ke sektor informal.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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