Judul: Pola Konsumsi Makanan, Status Gizi, Insomnia, dan Tekanan Darah pada Lansia di Kabupaten Klaten
Abstrak: Hipertensi pada lansia menjadi hal yang umum terjadi. Tekanan darah yang tinggi dapat memengaruhi kesehatan lansia. Penelitian ini bertujuan menganalisis pola konsumsi makanan, status gizi, insomnia, dan tekanan darah pada lansia di Kabupaten Klaten. Penelitian cross sectional study dilakukan terhadap 96 subjek lansia dengan rata-rata usia lansia laki-laki 69,6 ± SD 5,7 dan lansia perempuan dengan usia 66,4 ± SD 5,4 tahun. Pengambilan data dilakukan dengan pengukuran antrpometri, tekanan darah, dan wawancara. Sebagian besar hasil tekanan darah pada lansia masuk dalam kategori hipertensi tingkat I pada lansia laki-laki sebesar 29,2% dan hipertensi tingkat II pada lansia perempuan sebesar 41,7%. Mayoritas lansia laik-laki dan perempuan memiliki status gizi (Indeks Massa Tubuh) yang nomal. Hasil uji beda menunjukkan bahwa terdapat perebedaan yang signifikan (p<0,05) antara kelompok laki-laki dan perempuan pada variabel tingkat pendidikan dan konsumsi gula pada subjek. Selain itu, hasil penelitian menunjukkan bahwa ada korelasi antara status gizi (Indeks Massa Tubuh/IMT) (r=0,249, p=0,015), tingkat kecukupan energi (r=-0,411, p=<0,001), protein (r=-0,335, p=<0,001), karbohidrat (r=-0,336, p=<0,001), kalsium (r=-0,328, p=0,001), zat besi (r=-0,290, p=0,004), kalium (r=-0,225, p=0,027), dan konsumsi gula per hari (r=-0,305, p=0,003) dengan tekanan darah. Dengan demikian, hanya indeks massa tubuh yang berkorelasi positif dengan tekanan darah., Hypertension in the elderly is common. High blood pressure can affect the health of older adults. The study aims to analyze food consumption patterns, nutritional status, insomnia, and blood pressure of the elderly in Klaten. The cross-sectional study was conducted on 96 elderly subjects with a mean age of men aged 69.6 ± SD 5.7 years and women aged 66.4 ± SD 5.4 years. Data collection used anthropometric measurements, blood pressure, and interviews. The majority of blood pressure in the elderly is stage I hypertension in elderly men with a rate of 29.2% and stage II hypertension in elderly women with a rate of 41.7%. Most elderly men and women have normal nutritional status (body mass index). The results of the difference test showed that there was a significant difference (p<0.05) between the two groups of men and women in the variables of education level and sugar consumption of the subjects. In addition, the study showed that there is a correlation between nutritional status (body mass index/BMI) (r=0.249, p=0.015), level of energy adequacy (r=-0.411, p=<0.001), protein (r=-0.335, p=<0.001), carbohydrate (r=-0.336, p=<0.001), calcium (r=-0.328, p=0.001), iron (r=-0.290, p=0.004), potassium (r=-0.225, p=0.027) and daily sugar intake (r=-0.305, p=0.003) with blood pressure.
Keyword: blood pressure,, food consumption, insomnia, nutritional status

Judul: Keterkaitan Konsumsi Pangan, Status Gizi dan Status Kesehatan Lansia di Kota Bandung
Abstrak: Decreases of physiological function in the body will occur in the elderly population. As a result, the elderly body’s ability to work normally is decreased, especially in term of consuming foods. The lack of food consumption in the elderly will affect their nutritional status and increase the risks of the occurrence of anemia and health status problems. The objective of this study was to know the relationships of food consumption, nutritional status, and health status of the elderly living in nursing homes and families at the City of Bandung. A retrospective study of 115 elderly people was conducted and data collected on May 2012. The study showed that level of energy intake was normal, protein was lack, calcium was lack, iron was adequate, and vitamins were lack. Correlation test showed that there was no correlation among level of energy, protein, calcium, iron, vitamin B1, B2, B6 and vitamin C intake to health status (p>0.05). However, there was correlation among level of protein intake to nutritional status, level of vitamin B1 and B2 intake to health status, nutritional status and anemia to health status, as well as blood pressure to anemia (p<0.05).
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Judul: Isolasi Konstruksi dan Rekombinasi Gen Pengkode Reverse Transcriptase asal Simian Retrovirus-2 (SRV-2)
Abstrak: The availability of reverse transcriptase enzym as a valuable component for Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique has been providing by foreign companies. Therefore, it needs more cost and time to provide it. This study is an early step to produce in house reverse transcriptase rekombinant enzym from SRV-2. The purpose of this study was isolated, constructed, and rekombined SRV-2 reverse transcriptase gene to entry clone and destination vector. Isolation was done by PCR amplification technique using three pairs of primers, which are RT I (3282-3683), RTII (3261-3900), and RTIII (3169-4133). Construction and recombination in this study used Gateway method from Invitrogen. SRV-2 reverse transcriptase gene was isolated in this study with A-549 cell inoculated SRV-2 as source of virus. This gene amplify by PCR technique and resulted specific band for RTI 401 bp, RTII 639 bp, and RTIII 964 bp. Analysis of pENTR/SD/TOPO and pDEST17 indicated SRV-2 reverse transcriptase gene code has already inserted to this plasmid. This result indicated SRV-2 reverse transcriptase gene have isolated, constructed, and recombined to entry clone and destination vector.
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