Judul: Identifikasi Keragaman Genetik Gen Reseptor Hormon Pertumbuhan (GHR|AluI) pada Sapi Bali:
Abstrak: One of factor affects in growing is Growth Hormone Receptor (GHR). GHR is required for GH to carry out its effects on target tissues. The objective of the study was to estimate genetic diversity of the GHR|AluI in Bali, Limousin, Simmental and Pesisir cattle. Genotyping was performed on 248 animals, including 162 Bali, 21 Limousin, 17 Simmental and 48 Pesisir cattle breeds. Single Nucleotide Polymorphisms (SNP) has been found in exon 10, coding for the cytoplasmic domain of GHR, which was located at position 81 bp (A/G) induce amino acid substitutions Ser/Gly. Genotype frequencies of Bali cattle AA (0.988), GG (0.006) and AG (0.006) were evidenced for the GHR|AluI monomorphism, but largerly different from Limousin GG (0.667), AA (0.238) and AG (0.095), Simmental AG (0.529), GG (0.471) and AA (0.000), Pesisir AA (0.604), GG (0.375) and AG (0.021) were the evidenced of polymorphism. It was suggested that homozigosity (monomorphism) in Bali cattle could be affected by adaptability in extreme environmental conditions such as poor nutrition and improper manajement practice. It also could be affected by natural selection and phenotype plasticity phenomena.
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Judul: Identifikasi keragaman Gen Growth Hormone Receptor (GHR AluI) pada Sapi Friesian Holstein di BIB Lembang, BBIB Singosari, dan BET Cipelang.
Abstrak: Growth hormone receptor (GHR) is one factor affecting animal growth. GHR is required by growth hormone (GH) to carry out its effect on target tissues. The objective of this study was to identify polymorphism of the Growth Hormone Receptor gene in Holstein Friesian (HF) dairy cattle and beef cattle. Genotyping was performed on 126 animals. These consisted of HF 89 heads from BIB Lembang (17), BBIB Singosari (32), and BET Cipelang (40); as well as for 37 heads of four breeds of beef cattle including for Simmental (13), Limousin (14), Brahman (5), and Angus (5) for a comparison. Varian genetic of the GHR gene was identified by PCR-RFLP method using AluI restriction enzyme cutting the base position at AG|CT. Single nucleotide polymorphism (SNP) had been found in exon 10, coding for the cytoplasmic domain of GHR, which was located at position 81 bp (A/G) induced amino acid substitutions Ser/Gly. The product of amplification of GHR was 298 bp. Genotyping the GHR gene produced two alleles (A and G) resulting three genotypes (AA, AG and GG). The genotype AA was dominant in HF cattle from all locations, and also in Brahman and Limousin cattle from BET Cipelang. All of HF dairy cattle were in Hardy-Weinberg disequilibrium (χhit 2>χ0,05 2), in contrast, Simmental and Limousin were in Hardy-Weinberg equilibrium. The Hardy-Weinberg in Brahman and Angus values could not be calculated. Heterozigosity values in HF and beef cattle were low (ho<he). In beef cattle, Angus had highest heterozigosity value (0,600), while Brahman had the lowest heterozigosity value (0.000). The conclusion was that the variability of GHR|AluI gene can be used as a basic information for selection in growth caracteristic in the HF and beef cattle. Keywords: Beef cattle, Genetic polymorphism, GHR|AluI gene,
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Judul: Intrusion Detection System Untuk Pendeteksian Serangan Synflood Pada Protokol TCP/IP
Abstrak: Network Intrusion Detection System (NIDS) adalah pcnycdia pcrlindungan terhadap sistem jaringan komputer sclain dari mekanisme pcrlindungan yang telall diimplementasikan sebelumnya. Firewall scbagai salah satu alternatif pcrlindungan sistem jaringan memiliki kelemahan, yaitu hanya mampu mendcteksi paket data yang keluar dan masuk sistem jaringan yang ingin dilindungi. Pendekatan yang dilakukan oleh IDS adalah dengan melakukan proses audit terhadap segala aktivitas jaringan yang akan dilindungi. Dalam prakteknya, kejadian dalam sistem dinyatakan sebagai seknens dari pakel data yang melintasi atau memiliki tujuan ke sistem jaringan yang akan dilindungi. Penelitian ini mengenali pola serangan SYNflood untuk menentukanfinite state machine yang dapai dipergunakan dalam proses pcndeteksian.
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