Judul: Ekspresi Gen inaZ pada Beberapa Galur Bradyrhizobium japonicum Toleran Asam-Al
Abstrak: Penelitian terdahulu yang berlangsung di Laboralorium Mikrubiologi Jurusan Biologi, F.MIPA, IPB, lelah berhasil menyeleksi beberapa galur Bradyrhizobium japonicum toleran asam-Al yang diharapkan dapat dimanfaatkan sebagai simbion kedelai pada lattah asam. Untuk telaah kemampuan hidup bakteri tersebut di lingkungan alami dibutuhkan suatu penanda yang bersifat stabil.
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Judul: Seleksi Galur-Galur Bradyrhizobium Japonicum Toleran Asam-Aluminium
Abstrak: Bradyrhizobium japonicum merupakan bakteri penambat molekul nitrogen bebas (N2) yang bersimbiosis dengan akar tanaman kedelai dengan cara memben- tuk bintil. Bakteri ini berpotensi untuk meningkatkan produksi kedelai ter- utama pada lahan asam, melalui simbiosis antara B. japonicum toleran asam dengan kedelai yang juga toleran asam. Bradyrhizobium japonicum toleran asam yang diperoleh melalui skrining mikrobiologis dan kemampuannya berkompetisi dalam membentuk bintil akar dapat dievaluasi dengan menggunakan penanda molekuler yang stabil dan handal. Penanda molekuler yang dapat digunakan untuk menelaah aktivitas bakteri di habitat alaminya, antara lain adalah gen inaZ pada plasmid pJL1703, gen xylE pada plasmid pXYLE1, dan gen resistensi kanamisin dari Tn903 yang dibawa transposon mini-Tn5 (pUTmini-Tn5Kan). Penanda molekuler terpilih adalah yang paling stabil dan mampu diekspresikan oleh B. japonicum. Penelitian ini bertujuan : (i) menganalisis kemampuan galur-galur B. japo- nicum toleran asam mengekpresikan gen inaZ, gen xylE dan gen KanR serta kemampuannya dalam membentuk bintil akar, (ii) menentukan gen yang dapat digunakan sebagai penanda molekuler pada B. japonicum, (iii) menelaah kompetisi galur-galur B. japonicum toleran asam dalam membentuk binti akar kedelai dengan menggunakan penanda molekuler yang sesuai. Ketiga gen penanda tersebut masing-masing ditransfer ke B. japonicum dengan teknik konjugasi. Pemindahan gen inaZ dan gen xylE dilakukan secara triparental mating, sedangkan gen Kan secara diparental mating. Mating dilaku- kan di atas filter millipore dengan pori-pori 0.45 um di atas media Luria Agar (LA) yang dimodifikasi…dst
Keyword: Bradyrhizobium Japonicum, Bakteri  Bintil Akar, Konjugasi, Penanda Molekul

Judul: Thermal Characteristics and Dynamics of Tropical Cyclone over Western North Pacific Ocean: A Case Study of Typhoon Songda (Chedeng) 2011
Abstrak: The frequent occasion of Tropical Cyclone (TC) over Western North Pacific (WNP) Ocean lies from 10˚N to 26˚N and 121˚E to 170˚E. Sources of TC development come from warm ocean temperature where latent heat is released during condensation processes and subsequently concentrated in the boundary layer. Relative humidity at 850-700 hPa is responsible to produce the most intense storm. Meanwhile, weak vertical wind shear between 850-200 hPa is one of the dynamical factors that lead to the development of TC. Pressure level of 850-200 hPa is chosen to minimize the thermodynamic effect in the lower troposphere. Typhoon Songda (Chedeng in the Philippines) or TSC lasted from May 18th to 30th, 2011 and strengthened into powerful Super Typhoon stage between May 24th and 27th. Long curving track and the exhibition of all sequences of TC life cycle, TSC is then considered as an ideal case of TC. The objective of the study with case of TSC is to investigate the role of thermal characteristics and dynamics on TC intensity. The research is carried out by using Advanced Research of Weather Research and Forecasting model (WRF ARW model) version 3.3. Two domains are employed in the model. The first domain situate from 02˚N to 40˚N and 118˚E to 149˚E and cover spatial resolution () of 33.1 km. The second domain situate from 09˚N to 27˚N and 119˚E to 132˚E with of 11 km. Timestep of the model () is 120 seconds. The model is supported by the following physical parameterization, i.e., Yonsei University Scheme (YSU) in the boundary layer, Kain-Fritsch scheme (KF) in the cumulus, and WRF Single-Moment 3-class scheme in the microphysics. Initial meteorological condition prior to the formation of the cyclone is generated using WRF ARW standard-release model. Sensitivity of the model output is obtained from a selected single-parameter change scenarios, namely, 10 % to 20 % decrease and increase of wind shear initial data at 850-200 hPa, and by 10 % to 20 % decrease of relative humidity initial data at 850-700 hPa. The result showed that the negative correlations between wind shear and intensity are found in wind shear scenarios. Wind shears of 10 ms-1 which lead to strengthening of the wind intensity in the early period of Typhoon development are found in wind shear scenarios. The effect of wind shear changes on wind intensity is statistically significant in the 20 % wind shear scenarios. During mature stage, weak wind intensity occurred when wind shear increase as given in the WRF standard-release and the 20 % decreasing scenario. Different situation is found in the 20 % increasing scenario where wind shear tend to weak and lead to strong wind intensity at this stage. Meanwhile, the reduction of moisture supply at 850-700 hPa resulting from 20 % decreasing relative humidity play a major role in the weakening of wind intensity and reduces mechanical energy of the cycle process as much as 300 J kg-1.
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