Judul: Pemurnian Parsial Dan Karakterisasi Glukosa Oksidase dari Isolat Aspergillus niger (IPBCC.08.610)
Abstrak: The increasing application of enzymes in industry, healthcare and energy initiates production rise of enzymes from microorganisms, one of them are glucose oxidase from Aspergillus niger, which is now develop as enzymatic fuel cell. The aim of this study was to purified and characterized the enzyme glucose oxidase from Aspergillus niger (IPBCC.08.610). Partial purification using amonium sulfate precipitation and then followed by dialysis. The purification fold of dialysis enzyme was 6.17, specific activity enzyme was 43.17 Umg-1. In addition, the activity of glucose oxidase was stable at 25 oC. However, the result of ion stabilization of the enzyme showed that the activity of glucose oxidase reduced by the presence of Ag+ ion with relative activity was 4.8% in 1 mM and 2.1% in 5 mM. Thiourea inhibit the relative activity to 58.44% in 1 mM. On the other hand, the molecular weight of enzyme unit was estimated 160 kDa and there are 2 protein subunits of the glucose oxidase with approximately molecular weight 80 kDa. At the end, in the aspect of enzyme kinetics, glucose oxidase had Km and Vmaks value (glucose as a substrate) respectively 43 mM and 33.67 Umg-1
Keyword: partial purification, glucose oxidase, characterization, Aspergilus niger (IPBCC.08.610)

Judul: Pemurnian dan Karakterisasi Enzim Glukosa Oksidase dari Isolat Lokal Aspergillus niger (IPBCC.08.610)
Abstrak: Production of various enzymes using microorganisms is one way of developing microorganism biodiversity in Indonesia. For this study, glucose oxidase (GOD) which is widely available for use and purchase in the commercial market was used. This research intended to determine the optimum incubation time for the production of GOD from Aspergillus niger local isolate (IPBCC.08.610) and determine the specific activity of the crude extract and purified enzymes fraction. In addition, it sought to determine the optimum temperature and pH level in GOD reaction and establish the values of Km and vmax. Optimum incubation time for GOD production of A. niger (IPBCC.08.610) was 48 hours with initial velocity of the reaction and the specific activity at 0.80/minute and 3.21 U/mg. Ammonium sulfate with 80% saturation was optimum to precipitate the GOD protein with specific activity of the fraction at 22.18 U/mg and purity levels at 6.92 times. Specific activity of the fraction of dialysis was 21.25 U/mg and the level of purity was 6.63 times. Km and vmax values were 46 mM and 11 U/mg with optimal temperature and pH level for GOD reaction between 37 - 45°C and pH 5.5.
Keyword: 

Judul: Implementasi Space Time Composite (STC) Data Model Menggunakan Model Data Relasional
Abstrak: The data model is a concept that describes the data stored in the database and the relationship between the data to the user in logic. Development of data models is very important in the design of the database as the basis of the stage of database design to be made. This study aims to apply the concept of Space Time Composite (STC) on the Model Data storage vector data types that can store enough information and describes the process of drafting objects and objects spatiotemporal evolution over time. From the results of the test queries on the data model that have been created, queries in a system are acquired that can help display the information preparation of objects from time to time and know the history of the object polygon and arc that has undergone changes in topology.
Keyword: