Judul: Analisis Stabilitas Genotipe Temu Ireng dari Tiga Lokasi Berdasarkan Total Flavonoid, Saponin, dan Aktivitas Antioksidan
Abstrak: Temu ireng memiliki potensi yang baik untuk diaplikasikan secara luas sebagai obat tradisional, namun pemanfaatannya belum optimal karena tingkat produktivitasnya yang masih rendah. Tujuan penelitian yaitu menentukan genotipe tanaman temu ireng yang stabil dan memiliki aktivitas yang baik melalui pengaruh interaksi genotipe x lingkungan berdasarkan kandungan flavonoid, saponin, serta aktivitas antioksidan. Penelitian menggunakan 20 genotipe temu ireng sebagai sampel serta 2 varietas temu lawak kontrol yang ditanam pada 3 lokasi berbeda (Bogor, Cianjur dan Sukabumi). Pengujian total flavonoid dilakukan menggunakan metode AlCl3, sementara pengujian total saponin dilakukan menggunakan metode vanilin-asam sulfat, dan pengujian aktivitas antioksidan dilakukan menggunakan metode CUPRAC. Hasil penelitian menunjukkan adanya interaksi nyata antara faktor genotipe dan lingkungan pada taraf probabilitas 0%. Kandungan total flavonoid seluruh sampel pada ketiga lokasi tidak berbeda secara signifikan yang berkisar pada 0.02 hingga 0.04 mg QUE/g berat basah dan seluruh sampel merupakan genotipe stabil. Genotipe G1 dan G6 menghasilkan aktivitas antioksidan tertinggi secara signifikan pada ketiga lokasi sebesar 11.501 dan 10.201 mol TE/g berat basah, sementara seluruh sampel kecuali kedua genotipe tersebut merupakan genotipe stabil. Genotipe G5 mengandung total saponin tertinggi secara signifikan pada ketiga lokasi sebesar 146.369 mg DE/g berat basah, sementara genotipe G1, G2, G15, dan G16 merupakan genotipe stabil., Temu ireng has good potential to be widely applied as traditional medicine, but its’ utilization is not yet optimal due to its’ low productivity. The purpose of this study is to determine stable genotypes of temu ireng which had good activity through genotype and environmental interactions based on the content of flavonoids, saponins, and antioxidant activity. This study uses 20 genotypes of temu ireng as samples and 2 varieties of temu lawak as controls which were planted in 3 different locations (Bogor, Cianjur and Sukabumi). Total flavonoid testing was carried out using the AlCl3 method, while total saponin testing was carried out using the vanillin-sulfuric acid method and the antioxidant activity testing was carried out using the CUPRAC method. The results showed that there was a significant interaction between genotype and environmental factors at probability level of 0%. The total flavonoid content of all samples at the three locations did not differ significantly between 0.02 to 0.04 mg QUE/g fw, and all samples were stable genotypes. Genotypes G1 and G6 produced the highest antioxidant activity significantly at the three locations at 11.501 and 10.201 mol TE/g fw, while all samples except the two genotypes were stable genotypes. Genotype G5 had the highest total saponin content significantly at the three locations at 146.369 mg DE/g fw, while genotypes G1, G2, G15, and G16 were stable genotypes.
Keyword: antioxidant, flavonoid, genotype x environment interaction, saponin, temu ireng

Judul: Kandungan Flavonoid dan Aktivitas Antioksidan 20 Genotipe Rimpang Temu Ireng pada Musim Tanam Kedua
Abstrak: Temu ireng (Curcuma aeruginosa Roxb.) merupakan salah satu jenis tanaman golongan temu-temuan (Zingiberaceae) yang mengandung metabolit sekunder seperti flavonoid. Rendahnya produksi temu ireng disebabkan karena belum tersedianya varietas unggul dari temu ireng. Penelitian ini bertujuan menentukan genotipe terbaik dari total flavonoid dan aktivitas antioksidan yang dilihat dari segi stabilitas dan daya adaptasi dari 20 genotipe temu ireng yang ditanam pada tiga lokasi yang berbeda yaitu di Bogor, Sukabumi dan Cianjur. Tahapan penelitian meliputi pengujian kadar flavonoid dan aktivitas antioksidan metode FRAP serta daya adaptasi genotipe temulawak pada penanaman multilokasi dengan metode AMMI (Additive Main Effect and Multiplicative Interaction). Produktivitas kadar total flavonoid tertinggi dihasilkan sebesar 1.8980 mg QUE/g bobot kering di lokasi Bogor dan genotipe G4 sebesar 1.6170 mg QUE/g bobot kering. Nilai aktivitas antioksidan tertinggi dihasilkan di lokasi Bogor sebesar 18.5959 µmol TE/g bobot kering, dan genotipe G1 sebesar 30.6045 µmol TE/g bobot kering. Keragaman genotipe berpengaruh nyata terhadap kadar total flavonoid, akan tetapi tidak berpengaruh nyata pada aktivitas antioksidan. Interaksi lokasi dan genotipe tidak berpengaruh nyata terhadap kedua parameter uji. Genotipe yang stabil pada ketiga lokasi (Bogor, Cianjur, Sukabumi) adalah G5, G7, G8, G9, G17, G18 untuk kadar flavonoid, serta G1, G3, G4, G5, G7, G8, G10, G18, dan G20 untuk aktivitas antioksidan. Kata kunci: temu ireng, genotipe, flavonoid, antioksidan, stabilitas, Temu ireng (Curcuma aeruginosa Roxb.) is a type of plant that belongs to the group of plants belonging to the curvature family (Zingiberaceae) which contains secondary metabolites such as flavonoids. The low production of temu ireng is due to the unavailability of superior varieties of temu ireng. This study aims to determine the best genotype of total flavonoid and antioxidant activity in terms of stability and adaptability of 20 genotypes of temu ireng grown in three different locations, namely in Bogor, Sukabumi and Cianjur. The research stages included testing the levels of flavonoids and antioxidant activity using the FRAP method as well as the adaptability of the temulawak genotype in multilocation planting using the AMMI method. The highest productivity of total flavonoid content was 1.8980 mg QUE/g dry weight at the Bogor location and the G4 genotype was 1.6170 mg QUE/g dry weight. The highest antioxidant activity value was obtained at the Bogor location of 18.5959 mol TE/g dry weight, and the G1 genotype was 30.6045 mol TE/g dry weight. Genotype diversity had a significant effect on total flavonoid levels, but had no significant effect on antioxidant activity. The interaction of location and genotype did not significantly affect the two test parameters. Stable genotypes at the three locations (Bogor, Cianjur, Sukabumi) were G5, G7, G8, G9, G17, G18 for flavonoid content, and G1, G3, G4, G5, G7, G8, G10, G18, and G20 for activity antioxidants. Keywords: Curcuma aeruginosa, genotype, flavonoids, antioxidant, stability
Keyword: temu ireng, genotipe, flavonoid, antioksidan, stabilitas

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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