Judul: Perencanaan Partisipatif Pemanfaatan Taman Wisata Alam Buluhcina, (Kasus di Desa BuIuhcina, Kecamatan Siak BuIu Kabupaten Kampar, Provinsi Riau)
Abstrak: Buluh Cina Natural Park in Kampar District, Riau Province was initially Indigenous Forest (Hutan Adat) of Rimbo Tujuh Danau which has an area about 2.500 ha. Later, based on Governor Decree of Riau Province in 2007, most of the forest area used as a Natural Park area about 1,000 ha. Buluh Cina Natural Park have not been managed yet completely, but commonly it utilized by the communities to fulfill their daily needs. This study aims to determine the uses of forests and map the location of the utilization, furthermore describing the suitability of forest use practices with laws and regulations, as well as the use of participatory plan. This study, using data that obtained through multiple methods of data collection, which are review of secondary data, direct observation, interviews, focus group discussions (FGD) and mapping of areas. The results of this research show that Buluh Cina Natural Park utilization does not match with the provisions of existing law. The results of participatory planning which lead to the type of utilization that does not exploitative but the public has greater accessibility to forest management.
Keyword: Buluh Cina Natural Park, Participatory Planning, Forest Utilization

Judul: Studi Kasus Taman Nasional Gunung Halimun-Salak Desa Cirompang, Kec. Sobang, Kab. Lebak, Jawa Barat
Abstrak: Forest displace function in Cirompang Village from production forest become conservation forest generates polemic to community. Community accessibility to forest is also closed although it cannot be completely done until now. Forbidden access to the forest doesn’t decrease level of forest management because community finally realizes that the damage of forest is also their loss so that they decide to preserve nature using their own rule in area which they can manage by themselves. Government decision to make forest in Cirompang Village as conservation area will harm community. If it is closed, farmer will lose their farm area which large is a half from Cirompang village. This will be effected their income although there are others factors that influence their income, like their earnings from SPPT and from another sector besides agriculture. Participative mapping is selected to solve the problems of village area system. Participative map that contains boundary system and farm system can prove that they can manage their farm with their own mores without ignoring preservation of the nature.
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Judul: Excretory/secretory antigen of ascaridia galli L3 stage to trigger yolk immunoglobulin in laying hens
Abstrak: Ascaridiosis caused by the nematode Ascaridia galli. A study was carried out to characterize excretory/secretory antigen of A. galli from larval stage to trigger anti-ascaridiosis yolk immunoglobulin in laying hens. A. galli L3 were recovered from intestines of 100 heads chickens 7 days after oesophagus inoculation with 6000 L2. L3 recovered in this manner were cultured (5 – 10 ml-1) in flasks containing rosswell park memorial institute (RPMI) 1640 media, pH 6.8, without phenol red. Cultures were incubated at 370C in 5% CO2 and culture fluid was collected after 3 days in culture. To prepare excretory/secretory antigen, culture medium were concentrated with vivaspin 30.000 MWCO, and antigen protein concentration were counted as described in Bradford method. The molecular weight of excretory/secretory antigen was determined with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). Active immunizations were applied intra muscularly of chickens with an initial dose of 80 μg. The immunizations were repeated three times with dose of each 60 μg with an interval of one week. The first immunizations were excretory/secretory antigen mixed with fruend adjuvant complete and subsequently mixed with freund adjuvant incomplete. The chicken’s eggs were collected from 49 day after immunizations. Anti-ascaridiosis yolk immunoglobulin (IgY) was extracted from egg yolks by means of ammonium sulphate and purified using fast performance liquid chromatography (FPLC). The purity of anti-ascaridiosis IgY was determined by Bradford method (λ = 280 nm). Antibody was detected by agar gel precipitation test (AGPT) and enzyme linked immunosorbant assay (ELISA). Antigen were detected with immunohistochemistry. Chickens were challanged with dose of 1000 L2 A. galli. Passive immunizations were applied ten times with dose of each 0,875 mg IgY with an interval of one day intra orally. IgY anti-ascaridiosis was tested against A. galli survival in vitro.
Keyword: Unggas, Isolasi dan karakterisasi antigen, Purifikasi imunoglobulin yolk, Pengukuran antibodi ayam petelur, Metode Imunohistokimia, Ascaridiosis