Judul: Pertumbuhan dan kelangsungan hidup benih Ikan Gurame yang diberi protein rekombinan GH melalui perendaman dengan dosis berbeda
Abstrak: This research was conducted to determine the dose of rGH giant gouramy (r- OgGH) given through immersion that generate the highest growth of gouramy juvenile. The dose of rGH used was 10 mg / l, 20 mg / l, and 30 mg / l and without administration of rGH as a control. Fifty fries of 2 days after the yolk egg absorbtion were immersed into 200 ml of rGH solution for 60 min. Immersion was performed 3 times and applied once a week. The fish were reared in aquaria at stocking densities of 7 fish/l for 5 weeks and fed on Artemia nauplii and blood worm 2 times a day ad libitum. The results showed that the average body weight of 30 mg/l (0.3394 g) and 20 mg/l (0.3179 g) rGH-treated fish were higher (P<0.05) compared to that of 10 mg/l (0.2423 g) and control (0.1939 g). There was no body weight differences (P>0.05) between the fish treated with 20 mg/l and 30 mg/l of rGH, and between the fish treated with 10 mg/l rGH and control. Furthermore, the growth rate of rGH-treated fish was higher (P<0.05) compared with control. Survival of fry treated with rGH 30 mg/l (100%) was higher than the control and other treatments. Thus, immersion of fry in 30 mg/l rGH solution can be applied to enhance the growth of giant gouramy with a high survival rate.
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Judul: Respons benih ikan gurame (Osphronemus goramy) yang diberi hormon pertumbuhan rekombinan melalui oral pada dosis berbeda
Abstrak: This study was aimed to evaluate the response of giant gourami juvenile on oral administration of recombinant giant grouper growth hormone (rElGH). A total of 45 juvenile were fed with rElGH coated by hydropropylmethyl cellulose phthalate (HP55) and mix with commercial diet (35% protein content). Feeding fish with diet containing four different doses of rElGH (0 mg/kg feed, 0.3 mg/kg feed, 3.0 mg/kg feed, and 30.0 mg/kg feed) was performed in twice a week for 2 month. Fish were then maintained for 1 month without rElGH treatment. The results showed that biomass harvest in treatment 3.0 mg/kg (1,376.0±38.3 g) and 30.0 mg/kg (1,362.2±78.5 g) was similar, and the two treatments were 46.76% and 44.28% higher compared to control (977.6±96.7 g), respectively. In addition, rElGH treatment increased appetite, while artificial feed conversion ratio (1.18) was lower (P<0.05) by 23.72% compared to that of control (1.46). Oral administration of rElGH also increased hepatosomatic index (16.12%) and protein content (17%), while lipid content (21%) decreased compared to control. Similar in fish survival at end of experiment, insulin-like growth factor-1 and growth hormone receptor-1 gene expression levels in brain at 24 hours after feeding in rElGH treatment and control were obtained. Thus, orally rElGH administration was effective to be applied to enhance the growth of giant gourami juvenile.
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Judul: Analisis dan Implementasi Certificate Authorities (CA) pada Public Switched Telephone Network (PSTN)
Abstrak: Secure Telephone Protocol has ability to authenticate involved party and keep the secrecy of the message sent over the protocol. The secrecy of the message archieved by using symmetric key cryptographic scheme, and authentication archieved using public key cryptographic scheme. One of several party involved in the protocol and hold an important function called Certificate Authorities or CA. CA responsible for generating public key pair for other party involved in the protocol. When CA generates public key pair, several primitive cryptographic scheme used for guarantee the security of the key. Two of them are hash function SHA-1 and El-Gamal public key scheme embedded in Digital Signature Algorithm scheme. The prototype implementing the scheme above developed using Java programming language with JCE addition from Bauncy Castle. The analysis towards implemented prototype indicates that the prototype still has some flaws to be implemented in real world.
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