Judul: Analisis disparitas pembangunan antar wilayah di Kabupaten Sambas
Abstrak: Disparitas pembangunan antar wilayah seringkali menjadi permasalahan serius dan apabila tidak dieleminir secara bertahap dapat menimbulkan permasalahan yang lebih kompleks (seperti masalah kependudukan, sosial, ekonomi, politik dan lingkungan) serta dalam konteks makro sangat merugikan proses pembangunan yang ingin dicapai sebagai suatu bangsa yang utuh. Oleh sebab itu diperlukan pemahaman mengenai faktor-faktor penyebab terjadinya disparitas pembangunan antar wilayah. Faktor-faktor tersebut antara lain meliputi faktor biofisik/karakteristik wilayah (sumberdaya alam), sumberdaya buatan (ketersediaan sarana dan prasarana sosial-ekonomi), sumberdaya manusia, sumberdaya sosial, karakteristik struktur ekonomi wilayah, dan kebijakan pemerintah daerah. Dengan mengetahui faktor-faktor penyebab disparitas tersebut diharapkan dapat dikembangkan kebijakan dan strategi dalam rangka mengurangi tingkat disparitas yang terjadi. Bertolak dari hal tersebut di atas, maka penelitian ini bertujuan: (1) menentukan tingkat perkembangan/hirarki wilayah; (2) mengidentifikasi sektor unggulan pada tiap wilayah kecamatan; (3) menentukan tipologi dan karakteristik wilayah; (4) mengetahui tingkat disparitas pembangunan antar wilayah (kecamatan dan wilayah pengembangan), dan; (5) mengetahui faktor-faktor yang menyebabkan terjadinya disparitas pembangunan antar wilayah di Kabupaten Sambas.
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Judul: Analisis spasial disparitas pembangunan antar wilayah di provinsi Sumatera Barat
Abstrak: The phenomenon of disparity areas have become commonplace in the development of an area for various reasons. Development of region in the province of West Sumatra by naked eye there is a disparity in the region indicated. The objectives of this research are 1) determine the amount of disparity in development between regions, 2) identify the sectors held by each district / city; 3) determine the level of development and regional characteristics; 4) analyze the factors that cause disparities in development among regions; 5) to formulate strategies to address the problem of regional development disparities. Analytical methods used are Williamsons Index, Theill Index, Location Quotient Index, Shift Share Analysis, Scalogram Method, Entropy Index, Physical Analysis, Klassen Typology, Factor Analysis, Multiple Regression, and SWOT analysis. The analysis showed there are gaps in the province of West Sumatra which occurs predominantly among areas within the territory of the border region. Dominant sector in the district are agriculture, while in urban areas is service sector. The main factor that caused the region is an indicator of economic inequality and population indicators. The strategies that can be used to overcome the inequality are in development of other sectors of the economy of the agricultural sector and the optimization of the center point of growth and service center region.
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Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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