Judul: Construction of Gene Chitinase in Expression Vector by Method Gateway and Transformation to Agrobacterium sp.
Abstrak: Kelapa sawit merupakan tumbuhan industri penting penghasil minyak goreng, minyak industri, maupun bahan bakar. Usaha peningkatan produksi perkebunan kelapa sawit tersebut secara terus menerus dilakukan antara lain untuk mengatasi masalah hama dan penyakit yang disebabkan oleh Ganoderma spp yang dikenal dengan nama penyakit busuk akar. Salah satu cara untuk menanggulangi masalah ini adalah dengan menyisipkan gen penghasil zat yang berfungsi sebagai antijamur salah satunya yaitu gen penyandi kitinase. Penelitian ini bertujuan untuk membuat konstruksi gen penyandi kitinase dalam vektor ekspresi dengan metode Gateway yang cepat dan terarah. Gen penyandi kitinase yang berukuran 1500 pb dari penelitian sebelumnya oleh Novianthy (2009) terlebih dahulu disisipkan ke dalam vektor donor selanjutnya ke dalam vektor ekspresi. Pengujian gen penyandi kitinase yang telah tersisipkan ke dalam vektor ekspresi menghasilkan pita DNA berukuran 1500 pb yang ditunjukkan oleh elektroforegram PCR koloni gen penyandi kitinase. Vektor ekspresi yang telah tersisipi gen penyandi kitinase selanjutnya ditransformasikan ke dalam Agrobacterium tumefaciens galur AGL-0. Pengujian PCR koloni menunjukkan gen penyandi kitinase telah berhasil disisipkan pada A. tumefaciens. Gen penyandi kitinase siap ditransfer ke dalam tanaman.
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Judul: Konstruksi Gen Kitinase untuk Ketahanan terhadap Ganoderma pada Vektor Ekspresi pCambia 1303
Abstrak: Efforts to increase palm oil production face problems associated with pest and diseases caused by pathogenic fungus such as Ganoderma spp. A new approach to obtain palm oil transgenic is by inserting a gene encoding antifungi compound. One of genes is chitinase gene derived from Trichoderma. The research targets are to insert chitinase gene into expression vector pCambia 1303 between the 35S Cauliflower Mosaic Virus (CaMV) promoter and the root specific promoter. The Chitinase gene was cloned into pCAMBIA 1303 expression vector followed by transformation of recombinant plasmid into Escherichia coli XL-1 Blue and transferred into Agrobacterium tumefaciens. Results showed that the chitinase gene was successfully inserted into pCambia 1303. Restriction of the recombinant DNA using restriction enzymes Nco1 and Spe1 resulted in two bands of 1200 bp which is pCambia 1303 and of 1500 bp which is the chi gene. The recombinant DNA was successfully transformed into Agrobacterium tumefaciens using competent cell of AGLO strain. The success of transformation was analyzed by restricting recombinant DNA isolated from transformant colonies using NcoI and SpeI restriction enzymes.
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Judul: Penggunaan Teorema Homeomorphy 2-Manifold dan Teorema Euler Poincare pada Torus 𝑇�� dan Simplicial Complex 𝐾��
Abstrak: Two dimensional topological spaces are said to be homeomorphic if they have the same topological invariant, where one of topological invariant used is an Euler characteristic. Homeomorphy 2-Manifold’s Theorem and Euler Poincare’s Theorem are used to distinguish two topological spaces. Homeomorphy 2-Manifold’s Theorem uses Euler characteristic to identify two dimensional topological spaces. Euler Poincare’s Theorem is an alternative way to find Euler characteristic with Betti number, which is topological invariant as well. The objective of this paper is to investigate the homeomorphism of torus 𝑇����� and simplicial complex 𝐾�����. Topological space torus and simplicial complex 𝐾����� have the same Euler characteristic, which is two. Based on Homeomorphy 2-Manifold’s Theorem topological space torus 𝑇����� and simplicial complex 𝐾����� is homeomorphic.
Keyword: simplicial, torus, Euler, homeomorphism, topology