Judul: Content Analysis of The Instagram Account @Dearmoms.id on Fulfulling Parenting Information Needs
Abstrak: Penggunaan media instagram sebagai media informasi ditandai dengan banyak ditemukannya akun-akun komunitas yang berfokus untuk membagikan informasi, salah satunya informasi parenting. Tujuan dari penelitian ini adalah menganalisis konten akun instagram @dearmoms.id dalam memenuhi kebutuhan informasi parenting dan bagaimana hubungannya dengan faktor individu dan penggunaan media sosial. Penelitian ini menggunakan pendekatan kuantitatif dan didukung dengan data kualitatif. Metode yang digunakan dalam penelitian ini adalah metode survei dan analisis isi konten akun instagram @dearmoms.id. Responden dan konten dipilih dengan menggunakan metode nonprobability sampling dengan teknik purposive sampling. Hasil penelitian menunjukkan tidak terdapat kesesuaian antara konten dengan pemenuhan kebutuhan informasi parenting. Faktor individu yang berhubungan nyata dengan pemenuhan kebutuhan informasi adalah status usia anak. Penggunaan media sosial yang menghasilkan atensi yang tinggi memiliki hubungan yang nyata dengan pemenuhan kebutuhan informasi parenting
Keyword: instagram, information needs, parenting

Judul: Pengaruh Social Media Marketing dan Indikatornya Terhadap Customer Engagement Pada Magang Update (Studi pada pengikut akun Instagram @magangupdate di Jabodetabek)
Abstrak: Pemanfaatan media sosial sebagai salah satu media komunikasi dan berbagi informasi merupakan strategi pemasaran yang sedang banyak digunakan di mana dianggap mampu membangun keterlibatan dengan konsumen melalui konten menarik yang diunggah. Tujuan penelitian ini adalah untuk menganalisis pengaruh Social Media Marketing dan empat indikatornya yaitu Context, Communications, Collaboration, dan Connection terhadap Customer Engagement, serta mengidentifikasi indikator yang paling berpengaruh terhadap Customer Engagement pada akun Instagram Magang Update. Penelitian menggunakan sampel yaitu pengikut akun Instagram Magang Update yang di mana pengumpulan data menggunakan kuesioner yang diberikan kepada 100 responden. Data yang dikumpulkan kemudian dianalisis menggunakan metode analisis deskriptif dan Structural Equation Modelling-Partial Least Square (SEM-PLS). Hasil dari penelitian menunjukkan bahwa Social Media Marketing secara langsung berpengaruh positif dan signifikan terhadap Customer Engagement, sedangkan keempat indikator lain yaitu Context, Communications, Collaboration, dan Connection secara tidak langsung berpegaruh positif dan signifikan terhadap Customer Engagement, dan Connection adalah indikator yang paling mempengaruhi Customer Engagement pada akun Instagram Magang Update., The use of social media as a medium of communication and information sharing is a marketing strategy that is being widely used wich is considered capable of building engagement with consumers through interesting published content. The research objective is to analyze the effect of Social Media Marketing and its indicators that is Context, Communications, Collaboration, and Connection on Customer Engagement, also which indicators have the most influence on Customer Engagement on Magang Update Instagram account. Data collection is performed by distributing the questionnaire to 100 followers of Magang Update on Instagram. The data analysis uses descriptive analysis and Structural Equation Modeling by Partial Least Square (SEM PLS) method. The results of the study indicate that Social Media Marketing has a positive and significant direct effect on Customer Engagement, while the other four indicators there are Context, Communications, Collaboration, and Connection have a positive and significant indirect effect on Customer Engagement, and Connection is the most influential indicator on Customer Engagement on Magang Update Instagram account.
Keyword: customer engagement, Instagram, social media marketing

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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