Judul: Analisis modal sosial dalam rehabilitasi dan rekonstruksi desa pascabencana Merapi (Kasus Dusun Kinahrejo dan Pangukrejo, Desa Umbulharjo, Kecamatan Cangkringan, Kabupaten Sleman, Daerah Istimewa Yogyakarta)
Abstrak: Tujuan dari penelitian ini adalah untuk membandingkan kognitif dan struktural sosial ibu kota antara Dusun Kinahrejo dan Pangukrejo; untuk menganalisis hubungan antara modal sosial kognitif dengan aksi kolektif pembangunan perumahan; untuk menganalisis hubungan antara modal sosial struktural dengan tindakan kolektif pengembangan tempat tinggal; untuk menganalisis hubungan antara modal sosial kognitif dengan aksi kolektif pemulihan ekonomi; dan untuk menganalisis hubungan antara modal sosial struktural dengan aksi kolektif pemulihan ekonomi. Metode yang digunakan dalam penelitian ini adalah metode survei. Metode pengambilan sampel responden dilakukan dengan cara sederhana pengambilan sampel secara acak. Responden yang diambil sebanyak 60 responden. Teknik analisis data dalam penelitian ini menggunakan uji korelasi Rank Spearman. Penelitian ini menunjukkan suatu variabel tingkat kerjasama dalam modal sosial kognitif yang berhubungan dengan Rekompak aksi kolektif pembangunan tempat tinggal. Modal sosial struktural tidak memiliki terkait dengan aksi kolektif Rekompak pembangunan pemukiman di Kinahrejo Dukuh. Hasil uji statistik Dusun Pangukrejo menunjukkan derajat solidaritas variabel dan tingkat kerjasama (modal sosial kognitif) berhubungan dengan sapi perah program pemulihan perekonomian. Dalam modal sosial struktural, tingkat partisipasi sebesar variabel organisasi anggota yang berhubungan dengan program ekonomi sapi perah di Dusun Pangukrejo.
Keyword: Cognitive social capital, Structural social capital, Collective action

Judul: Hubungan Aksi Kolektif Berorientasi Identitas dengan Implementasi Prinsip Pengembangan Masyarakat Berbasis Ekosistem (Kasus: Program Rehabilitasi Hutan Mangrove oleh Komunitas Kompilasi, Desa Ujungjaya, Kecamatan Sumur, Kabupaten Pandeglang, Provinsi Banten)
Abstrak: Pengembangan masyarakat berbasis ekosistem dipandang sebagai sebuah strategi dalam mencapai pembangunan berkelanjutan sekaligus pemenuhan kebutuhan hidup masyarakat. Pengembangan masyarakat yang mengedepankan prinsip pemberdayaan tidak dapat terwujud tanpa adanya kerjasama masyarakat dalam wujud aksi kolektif. Tujuan dari penelitian ini adalah menganalisis hubungan antara aksi kolektif dan implementasi pengembangan masyarakat perbasis ekosistem, serta keterkaitannya dengan keberlanjutan program dan lingkungan. Metode yang digunakan dalam penulisan ini adalah data kuantitatif yang didukung dengan data kualitatif dalam melengkapi data. Data kuantitatif diolah menggunakan uji korelasi Pearson Product Moment dengan pemilihan responden sebanyak 30 orang menggunakan metode sensus. Hasil penelitian ini menunjukkan: 1) Lebih dari 90 orang responden menampilkan tingkat aksi kolektif berupa identifikasi kolektif, solidaritas, dan komitmen, yang termasuk Kuat; 2) Sekitar 60% orang responden memiliki intensitas implementasi prinsip pengemas berbasis ekosistem Tinggi, namun masih ada 30% yang Sedang yaitu pada organisasi SDM, data, dan kerjasama; serta 3) Ada korelasi antara tingkat aksi kolektif berorientasi identitas dengan intensitas implementasi prinsip pengembangan masyarakat berbasis ekosistem sebesar 0,728 (p<0,05)., Ecosystem-based community development is a strategy to achieve sustainable development as well as to meet the needs of people's lives. Community development that prioritizes the principle of empowerment cannot be fulfilled without cooperation in the form of collective action. Therefore, the objectives of this study are to determine the correlation between collective action and the implementation of ecosystem-based community development principles, and also its relationship with program and environmental sustainability. The research will use the quantitative data with the support of qualitative data. Quantitative data was processed using Pearson Product Moment Correlation Test with 30 respondents selected by the census method. The results of this study indicates that: 1) More than 90 respondents displayed a level of collective action in the form of collective identification, solidarity, and commitment, which included Strong; 2) Around 60% of respondents have a high intensity of implementing ecosystem-based packaging principles, but there are still 30% who are moderate, namely in HR, data, and collaboration organizations; and 3) There is a correlation between the level identity-oriented of collective action and the intensity of implementation of ecosystem-based community development principles of 0.728 (p<0.05).
Keyword: collective action, community development, ecosystem

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable