Judul: Perancangan Galeri Terbuka pada Taman Mini Indonesia Indah (TMII)
Abstrak: The monotonous of galleries in Indonesia led to the lack of special space for outdoor art works exhibition. This study is required to create new work in the field of landscape architecture in the form of gallery design with a different concept. The purpose of this study was to identify and analyze the kind of art that can be displayed in the open gallery requirements then develop the design concept of open gallery as an example of the new show room by designing open gallery at Taman Mini Indonesia Indah (TMII). The methods used in this study through two approaches including social approaches such as interview and questionnaire then biophysical approaches such as observation, spatial analysis, analysis of the carrying capacity and climate analysis. Open gallery is a form of show room that can accommodate a variety of art activities in open spaces such as exhibition of the installation art works, sculpture, street art, and other various works of art that can not be exhibited in the indoor gallery. Open gallery also has support spaces for exhibition such as art performance space, workshop and discussion space, art shops, cafeteria as well as warehouse.
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Judul: Desain Artwork pada Taman Pulau dan Median Jalan di Jalan Medan Merdeka Jakarta
Abstrak: Ruang terbuka merupakan bagian dari kebutuhan masyarakat untuk bersantai. Ruang terbuka dapat memiliki banyak bentuk dan fungsi, diantaranya adalah taman pulau dan median jalan. Taman pulau dan median jalan memiliki potensi sebagai media display untuk membentuk karakter dan memberikan mental map bagi suatu kawasan. Karakter suatu kawasan dapat dibentuk dengan unsur-unsur di dalamnya, salah satunya dengan penempatan artwork. Artwork dapat menjadi elemen mental map yang berfungsi untuk menavigasi seseorang untuk berpindah dari satu titik ke titik lain, terutama di kota besar seperti Jakarta. Kawasan Medan Merdeka dikelilingi oleh taman pulau dan median jalan, hingga saat ini masih belum memiliki karakter dan daya tarik untuk membentuk mental map bagi penggunanya. Penelitian ini bertujuan untuk membuat desain artwork yang dapat membentuk karakter dan mental map di kawasan jalan Medan Merdeka. Kegiatan ini dilakukan di kawasan Medan Merdeka selama 16 minggu dengan metode observasi langsung dan wawancara. Output dari penelitian ini meliputi desain artwork di taman pulau dan median jalan.
Keyword: artwork, median jalan, mental map, ruang terbuka, taman pulau

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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