Judul: Kloning dan Karakterisasi Gen Penyandi α-amilase (amyA) Aspergillus niger serta Konstruksi Ekspresi dengan Teknologi Gateway
Abstrak: Cloning of the a-amylase gene of Aspergillus niger chromosomal DNA on pDEST 24 plasmid to increase the enzyme activity has been investigated. The transformation was conducted with several steps, including isolation of the messenger RNA, ligation by T4-DNA ligase, transformation of the recombinant DNA, and selection of the transformants using PCR colony. Gene encoding α- amylase isolated using RT-PCR (Reverse Transcriptase Polymerase Chain Reaction technique) and inserted into the pGEM-T Easy vector. Electrophoresis of the PCR products showed that the gene has a size of approximately 1650 bp. BLAST analysis of the DNA sekuen showed that this gene is homologous with the gene encoding α-amylase accessible from the gene bank. Expression of the gene encoding α-amylase of Aspergillus niger used gateway cloning technology. It showed that this gene has been successfully cloned in the cells of Escherichia coli through destination vector (pDEST 24). Electrophoresis of the PCR colony showed that the gene has a size of approximately 1650 bp.
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Judul: Karakterisasi dan Kloning Gen Penyandi Glukosa Oksidase (GGOx) dari Aspergillus niger IPBCC 08.610.
Abstrak: Glukosa oksidase digunakan dalam berbagai kegiatan industri maupun pengembangan teknologi enzymatic fuel cell. Penelitian sebelumnya menunjukkan bahwa isolat lokal Aspergillus niger IPBCC 08.610 dapat menghasilkan glukosa oksidase. Penelitian terkait gen penyandi glukosa oksidase (GGOx) dari isolat ini belum dilakukan. Penelitian ini bertujuan mendapatkan gen penyandi glukosa oksidase dari isolat A. niger IPBCC 08.610, mendapatkan karakteristik gen meliputi urutan dan panjang basa serta pola restriksinya, dan mendapatkan koloni trnasforman E. coli DH5α yang membawa gen. Gen penyandi glukosa oksidase didapatkan dari amplifikasi DNA genom dengan primer spesifik dan dikarakterisasi melalui teknik restriksi dengan endonukelase EcoRI dan PstI. Selain itu, gen juga disisipkan pada vektor pGEM®T-Easy dan ditransformasikan pada sel kompeten E. coli DH5α agar dapat dilakukan pengurutan basa nitrogen (sekuensing). Gen penyandi glukosa oksidase dari A niger IPBCC 08.610 memiliki ukuran sebesar 1818 bp. Gen ini dapat direstriksi menjadi dua fragmen berukuran 908 bp dan 980 bp dengan EcoRI. Terdapat tiga koloni transforman yang membawa gen penyandi glukosa oksidase.
Keyword: Aspergillus niger, endonuklease restriksi, gen penyandi glukosa oksidase, kloning, sekuensing

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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