Judul: Analisis Biplot untuk Menelaah Persepsi Kedekatan Atribut pada Ritel dan Produk Barang
Abstrak: Fungsi ritel yang penting bagi konsumen dalam berbelanja produk eceran ternyata tidak sejalan dengan pertumbuhan penjualannya. Pertumbuhan penjualan ritel yang menurun, sangat berkaitan dengan penurunan pertumbuhan penjualan barang eceran. Penelitian ini bertujuan menelaah atribut dari bauran ritel dan bauran pemasaran yang dipersepsikan dekat (penting) oleh konsumen terhadap delapan objek penelitian; hipermarket, supermarket, department store, mie instan, susu bubuk, perlengkapan alas kaki, dan smartphone. Kedekatan atribut dengan objek pada penelitian ini di analisis menggunakan analisis biplot yang berfungsi melihat proximity antara variabel peubah dengan objek penelitian. Hasil dari penelitian ini adalah; atribut layanan antar dekat dengan ritel hipermarket dan supermarket; atribut pelayanan karyawan dekat dengan department store; rasa dan tekstur dekat dengan produk mie instan; ketersediaan ukuran dekat dengan perlengkapan alas kaki; dan atribut merek, fitur, dan harus dibeli di toko khusus dekat dengan objek smartphone berdasarkan persepsi konsumen.
Keyword: analisis biplot, atribut bauran pemasaran, atribut bauran ritel, produk barang, ritel

Judul: Analisis Disruptif Teknologi Terhadap Bauran Pemasaran 9P (Belanja Daring dan Belanja Konvensional).
Abstrak: Disruptif teknologi merupakan era di mana pelaku usaha harus peka terhadap perubahan yang terjadi. Teknologi yang terus berkembang menghadirkan inovasi yang tak terduga. Pelaku usaha yang tidak melakuan perubahan memungkinkan akan menurunkan pangsa pasar. Penelitian ini bertujuan menelaah atribut bauran pemasaran yang dianggap penting oleh konsumen terhadap objek penelitian, dengan objek penelitian belanja daring dan belanja konvensional. Belanja dalam jarigan dikategorikan dengan belanja daring, dan belanja konvensional dikategorikan menjadi dua yaitu belanja modern dan belanja tradisional. Kedekatan atribut dengan objek pada penelitian ini di analisis menggunakan analisis biplot yang berfungsi melihat proximity antara variabel peubah dengan objek penelitian. Hasil dari penelitian ini adalah; variabel bauran produk, tempat, promosi, bukti fisik, dan pembayaran dekat dengan belanja modern dan daring. Variabel bauran personal dekat dengan belanja modern dan belanja tradisional. Variabel harga dan proses dekat dengan belanja tradisional. Variabel bauran produktivitas dan kualitas dekat dengan belanja daring.
Keyword: analisis biplot, atribut bauran pemasaran 9P, belanja daring, belanja konvensional

Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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