Judul: Deteksi Keberadaan Antibodi Anti Diare (Escherichia coli dan Salmonella Enteritidis) Dan Anti Flu Burung (H5N1) Pada Kuning Telur Ayam Isa Brown Yang Diberi Perlakuan Pemanasan Bertingkat
Abstrak: This study was aimed to detect the existence of antibodies (immunoglobulin Y) specific to Escherichia coli, Salmonella Enteritidis, and Avian influenza (H5N1) in eggs after heating/boiling process. The treatment is by heating or boiling the whole eggs complete with shells at a temperature of 60°C, 70°C, 80°C, 90°C and 100°C for 5 minutes. The Eggs were tested that containing antibodies of anti-diarrhea and anti-avian influenza. This research used Agar Gel Precipitation Test (AGPT) method to detect antibodies of anti-diarrhea and hemagglutination inhibition (HI) test to detect antibodies of avian influenza. The results showed that the presence of antibodies (IgY) can still be detected by using AGPT and HI method in the eggs which be heated at 60°C, 70°C and 80°C for 5 minutes. Antibodies specific for Escherichia coli, Salmonella Enteritidis, and avian influenza (H5N1) could survive in the eggs complete with eggshells which be heated in the range of temperature 60–80°C. While on heating 90°C and 100°C has not detected the presence of antibodies specific because antibodies which is consist of protein was denatured. It could be concluded that the higher of egg heating temperature, IgY biological activity were decrease.
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Judul: Deteksi Imunoglobulin Y Pada Telur Asin Anti Diare Dan Flu Burung Dengan Metode Agar Gel Precipitation Test (AGPT) Dan Hemagglutination Inhibition Test (Hi Test)
Abstrak: This study was conducted to detect specific IgY (anti Enteropathogenic Escherichia coli (EPEC), anti Salmonella Enteritidis, and anti Avian Influenza Virus H5N1) in salted egg. The laying hens were vaccinated with EPEC and Salmonella Enteritidis inactive-vaccine and AI H5N1 vaccine (IPB-Shigeta). Nine eggs were salted by NaCl solution (1:2). The eggs were salted by three times of long-term salting (10 days, 15 days, and 20 days). The IgY was detected using AGPT and haemagglutination inhibition (HI) test. The AGPT result showed that specific IgY of EPEC and Salmonella Enteritidis detected at 10 days and 15 days long-term salting. The mean titer of anti AI-IgY (over 24) was detected using by HI at all long-term salting. In conclusion, salted egg yolk still have specific IgY which is potential for production functional salted egg.
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Judul: Hubungan Graf, Ruang Kebebasan, dan Dual Graf Pada Struktur Cycle dan Struktur Cutset
Abstrak: Ruang kebebasan adalah suatu ruang yang pembentukannya berdasarkan konsep sifat bebas linear dan konsep graf. Ruang kebebasan terdiri dari koleksi himpunan bebas-himpunan bebas yang merupakan himpunan bagian dari suatu himpunan berhingga, dan himpunan berhingga itu sendiri secara umum dapat dikatakan mirip dengan himpunan sisi pada graf. Himpunan bagian yang bukan himpunan bebas disebut himpunan takbebas. Koleksi himpunan bebas-himpunan bebas disebut struktur kebebasan. Himpunan bebas maksimal disebut basis dan himpunan takbebas minimal disebut sirkuit. Dari sebuah graf terhubungkan dapat diperoleh dua buah struktur kebebasan yaitu struktur cycle dan struktur cutset. Struktur cycle adalah himpunan sisi yang tidak memuat cycle, sedangkan struktur cutset adalah himpunan sisi yang tidak memuat cutset. Koleksi basis dari struktur cicle merupakan spanning Tree-spanning Tree pada graf, sedangkan koleksi basis dari struktur culset "merupakan komplemenkomplemen dari spanning Iree. Dengan melihat basis, hubungan graf dan fuang kebebasan dapat dinyatakan sebagai berikut, struktur cycle dan struktur culset merupakan dual struktur kebebasan.
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