Judul: Produksi lipid dan karbohidrat ganggang mikro asal sawah dan perairan tawar yang dikultivasi pada skala lapang
Abstrak: Production of bioenergy as an alternative for oil fuel requires biomass as raw material. With an extremely rapid growth rate, microalgae is potential to be developed as a source of bioenergy by extracting lipids and carbohydrates from its biomass. This research was initiated by a laboratory-scale cultivation of 4 isolates of microalgae (ICBB 9111, ICBB 9112, ICBB 9113, and ICBB 9114) in 50 ml medium with 5 different concentrations, i.e. 0.75 times the standard media M4 composition (0.75 M4), M4, 1.25 M4, 1.50 M4, and 1.75 M4. The composition of M4 standard media (g/L): 1.5 NaNO3, 0.049 K2HPO4, 0.0366 MgSO4, 0.0271 CaCl2, 0.006 citric acid, 0.006 Fe-ammonium citrate, 0.001 EDTA, 0.02 Na2CO3 and 1 ml micro nutrient with composition (g/L): 2.86 H3BO3, 0.1485 MnCl2, 0.1245 ZnSO4, 0.0506 Na2MoO4.2H2O, 0.0506 CuSO4, and 0.043 CoCl2.6H2O. The laboratory scale growth-rates of microalgae at different compositions of standard media were varied. Based on the results of analysis of variance, Duncan's Multiple Range Test, and growth chart, the optimum medium for the field-scale cultivation were 0.75 M4 for microalgae ICBB 9112 and ICBB 9114, and M4 for microalgae ICBB 9111 and ICBB 9113. The growth rate of microalgae at field scale was faster than those at laboratory one. The average biomass productivity of microalgae ICBB 9111, ICBB 9112, ICBB 9113, and ICBB 9114 after cultivated for 2 days at field scale were, respectively, 0.28, 0.21, 0.26, and 12.32 g/L. The highest lipid production was produced by isolate ICBB 9114 (32% w/w) at 0.75 M4 medium, and the highest carbohydrate production was obtained from isolate ICBB 9113 (44% w/w) at M4 media. This result indicates that when the microalgae lipid production is high, the corresponding carbohydrate production is lower and vice versa.
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Judul: Seleksi dan karakterisasi ganggang mikro indigen air tawar sebagai penghasil karbohidrat untuk energi terbarukan
Abstrak: The objective of this research is to explore and isolate indigenous micro algae that have a high carbohydrate producer as potentially for bioethanol production. The exploration from indigenous fresh water fifteen micro algae carried out in a BG 11 media and resulted four different types of micro algae such as ICBB-CC (9111, 9112, 9114 and 6354 PLB). By morpholgical identification, they were identified as Crucigenia quadrata, Scenedesmus bijuga, Chlorella vulgaris, and Chlorella vulgaris respectively. They were cultivated in a 5 L reactor using three types media of BG 11, MBM and PHM. The fastest growing isolate was ICBB 9111 of C. quadrata in MBM medium. The production biomassa of four isolates were developed in the raceway of 90-100L using MBM modifications medium with TSP aims , because of media has a value of optical density (OD) which increased from 0.09 to 0,200 within 3 (three) days. Harvesting is done from the OD value ≥ 0.6 continuously and harvesting stopped at OD values ≤ 0.5. Harvesting by means of filtration and drying naturally at room temperature. Temperature micro algae in ponds ranging channel 30-35 oC and maintained volume in such a way as early cultivation (by adding water to take advantage of existing nutrients). Production of dried-biomass/L/d and the results of the proximate analysis of carbohydrates into four isolates : Crucigenia quadrata ICBB 9111, Scenedesmus bijuga ICBB 9112, Chlorella vulgaris ICBB 9114 and Chlorella vulgaris PLB 6354) is 0,038 (42.27% dry weight), 0,035 (21.30% dry weight), 0.019 (16.66% dry weight) and 0,018 (17.22% dry weight). Production of dry biomass / L / day and the results of the proximate analysis of carbohydrates into four isolates ICBB-CC (9111, 9112, 9114 and 6354 PLB) is 0,038 (42.27% dry weight), 0,035 (21.30% dry weight), 0.019 (16.66% dry weight) and 0,018 (17.22% dry weight). Result of the selection of four types of micro algae ICBB-CC (9111, 9112, 9114 and 6354 PLB,) with modifications MBM TSP aims to get local micro algae isolates that have high carbohydrate content potentially for the production of ethanol.Optimal conditions for hydrolysis process 48 hours using a commercial enzyme that includes two stages: liquefaction process using enzymes α-amylase 600U at 95 ° C with a pH of 6 while for the saccharification process using enzymes amiloglukosidase 145U at a temperature of 60 ° C, pH 4.5 and 120 rpm rotation .Ethanol content of 40-hour fermented using S. Cerevisiae each sample, namely: 9111 amounted to 50.47 ppm, 105.86 ppm for 9112, 9114 amounted to 53.38 ppm and 41.17 ppm of PLB 6354.
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Judul: Prolapsus uteria dan penanggulangannya
Abstrak: Prolapsus uteri adalah penyembulan mukosa uterus dari badan melalui vagina. Penyembulan ini dapat total dan dapat pula sebagian. Umumnya terjadi setelah melahirkan. Prolapsus uteri disebabkan karena pada saat melahirkan, hormon oxytocin yang dihasilkan oleh kelenjar hipofise posterior sangat meningkat, sehingga gerak peristaltik, perejanan urat daging dan diafragma berlangsung terus menerus dengan kuat, meskipun foetus telah lahir. Faktor predisposisi prolapsus uteri adalah hewan yang telah berumur tua dan selalu di kandangkan sepanjang tahun, sehingga menyebabkan relaksasi ligamentum penggantung uterus. Infeksi yang disebabkan oleh kuman penyebab abortus serta batuk yang kronis lebih mendorong terjadinya prolapsus uteri.
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