Judul: Evaluasi Penerapan Konsep Kota Hijau di Kota Tangerang
Abstrak: Development of urban areas grew rapidly. Increasing physical development of the city cause the imbalance between urban growth and the attempt to increase the quality of the environment. The condition makes an uncomfortable city to live. Therefore the future urban planning could be directed to use the green city concept. Green city is a healthy city that considers the aspects of ecological planning. This research was conducted in Tangerang city by using Gap Analysis to find out the actual condition in applying the concept of green cities and comparing it with the ideal condition. The evaluation showed that implementation of green city concept in Tangerang city was still continue. It could be seen from the implementation of eight indicators of green city which had not been maximized, and spreaded through the region.
Keyword: urban landscape, liveable city, green city

Judul: Evaluasi Penerapan Konsep Kota Hijau di Kota Tangerang Selatan
Abstrak: Aktivitas yang terjadi di lanskap perkotaan menyebabkan berbagai masalah lingkungan. Kota Tangerang Selatan merupakan kota penyangga bagi Jakarta karena lokasinya memiliki berbagai permasalahan yang belum terselesaikan. Kota hijau atau kota berkelanjutan adalah kota yang diintegrasikan dengan lingkungan lokal yang menggunakan metode keberlanjutan dalam memproduksi energi, daur ulang air, membuang sampah, dan mengurangi polusi terhadap air, lahan, dan udara. Penelitian ini bertujuan memberi penilaian terhadap lanskap kawasan perkotaan khususnya di Kota Tangerang Selatan dalam menerapkan konsep kota hijau dengan melihat peran dari pemerintah, pengembang swasta, dan masyarakat serta mengukur tingkat kebahagiaan masyarakat. Metode yang digunakan pada penelitian adalah metode survey lapang mengacu pada Asian Green City Index. Terdapat delapan kategori keberlanjutan dengan hasil evaluasi berupa kinerja Kota dalam menerapkan kota hijau adalah Energy and CO2 (42.40%), Land use and buildings (49.17%), Transport (51.15%), Waste (38.80%), Water (51.75%), Sanitation (47.96%), Air Quality (69%), dan Environmental Governance (66.9%). Hasil keseluruhan Kota Tangerang Selatan dalam menerapkan kota hijau adalah 52.15% dan kedudukan Kota Tangerang Selatan dalam tabel performa ada di rentang rata-rata. Tingkat kebahagiaan masyarakat diperoleh dari hasil kuesioner dan wawancara. Tingkat kebahagiaan masyarakat yaitu 40% sangat bahagia, 55% bahagia, dan 5% kurang bahagia.
Keyword: asian green city index, kebahagiaan, keberlanjutan, kota hijau, lanskap perkotaan

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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