Judul: Usulan Perencanaan Struktur Atas Green Building Departemen Teknik Sipil dan Lingkungan, Institut Pertanian Bogor
Abstrak: dan Lingkungan (SIL), Institut Pertanian Bogor. Penelitian bertujuan untuk mendesain struktur atas gedung beton bertulang yang efisien, dan merencanakan dimensi struktur yang aman untuk menopang beban rencana. Penelitian ini menggunakan SAP2000, perencanaan gedung beton bertulang berdasarkan pada SNI 1727:2013, SNI 1726:2012, SNI 2847:2013 dan laporan basic design green building Departemen SIL. Dari hasil penelitian diperoleh dimensi kolom sebesar 80 x 60 cm, dengan tulangan pokok 18D19. Balok dibagi menjadi lima segmen dengan selimut beton 4 cm dan sengkang D10. Balok pada kantilever: 40 x 30 cm, tulangan D22. Balok membujur 9 m berukuran 35 x 25 cm D19. Balok membujur dibentang 13 m: 40 x 30 cm D22. Balok pada tangga: 30 x 20 cm D16. Balok arah melintang: 35 x 25 cm D22. Bracing kantilever 30x20 cm, tulangan utama 6D12, tulangan sengkang D10-100. Plat dan tangga menggunakan plat bondek, ketebalan 12 cm dan tulangan Ø8-250. Tangga memiliki kemiringan 18.4o.
Keyword: beton bertulang, green building, perencanaan, struktur atas

Judul: Usulan Perencanaan Struktur Atas Green Building Departemen Teknik Sipil dan Lingkungan, Institut Pertanian Bogor
Abstrak: Penelitian ini dilakukan berdasarkan kebutuhan gedung Departemen Teknik Sipil dan Lingkungan (SIL), Institut Pertanian Bogor. Penelitian bertujuan untuk mendesain struktur atas gedung beton bertulang yang efisien, dan merencanakan dimensi struktur yang aman untuk menopang beban rencana. Penelitian ini menggunakan SAP2000, perencanaan gedung beton bertulang berdasarkan pada SNI 1727:2013, SNI 1726:2012, SNI 2847:2013 dan laporan basic design green building Departemen SIL. Dari hasil penelitian diperoleh dimensi kolom sebesar 80 x 60 cm, dengan tulangan pokok 18D19. Balok dibagi menjadi lima segmen dengan selimut beton 4 cm dan sengkang D10. Balok pada kantilever: 40 x 30 cm, tulangan D22. Balok membujur 9 m berukuran 35 x 25 cm D19. Balok membujur dibentang 13 m: 40 x 30 cm D22. Balok pada tangga: 30 x 20 cm D16. Balok arah melintang: 35 x 25 cm D22. Bracing kantilever 30x20 cm, tulangan utama 6D12, tulangan sengkang D10-100. Plat dan tangga menggunakan plat bondek, ketebalan 12 cm dan tulangan Ø8-250. Tangga memiliki kemiringan 18.4o.
Keyword: beton bertulang, green building, perencanaan, struktur atas

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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