Judul: Evaluasi Kesadaran Adopsi Anjing dan Persepsinya Terhadap Pemilik Anjing
Abstrak: Dog adoption is a process performed by potential dog adopters to adopt their desired dog after going through a selection of process. Often dog adoption process is carried out in shelters and rescue groups. Dog adoption is practiced mostly in developed countries that prefers to adopt due to several reasons such as helps to promote good mental health, a great companionship, and builds great interaction. This study aims to examine the perceptions of potential adopters on the criteria of adopting, choosing a dog and the information that was concerned before and after pet selection process. An online questionnaire was carried out to determine the perceptions of dog owners. Out of 150 participants, 132 prefers to adopt an animal, whereas 8 of them prefers to buy. The highest picked reason to adopt a pet was to provide the pets a forever home. 69.7% of participants who wishes to adopt prefers to visit the animal before adoption. The concerned factor upon adoption was the dog’s health issue and prefers to adopt puppies (less than 6 months). 99.3% were aware of dog abuse and its welfare., Adopsi anjing adalah suatu proses yang dilakukan untuk mengadopsi anjing yang diinginkan oleh calon pengadopsi (adopter) setelah melalui proses seleksi. Seringkali proses adopsi anjing dilakukan di tempat penampungan dan kelompok penyelamat. Di negara maju adopsi anjing banyak dipraktikkan karena beberapa alasan seperti meningkatkan kesehatan mental yang baik, meningkatkan nilai persahabatan, dan membangun interaksi dengan manusia. Penelitian ini bertujuan untuk menguji persepsi calon adopter terhadap kriteria adopsi, pemilihan anjing dan informasi yang diperlukan sebelum dan sesudah proses seleksi hewan peliharaan. Kuesioner online dilakukan untuk mengetahui persepsi pemilik anjing. Dari 150 peserta, 132 lebih memilih untuk mengadopsi hewan sedangkan 8 di antaranya lebih memilih untuk membeli anjing. Alasan pilihan tertinggi untuk mengadopsi hewan peliharaan adalah untuk memberikan rumah hewan peliharaan untuk selamanya. 69,7% partisipan yang ingin mengadopsi lebih suka mengunjungi hewan sebelum diadopsi. Faktor yang menjadi perhatian pada saat adopsi adalah masalah kesehatananjing dan lebih memilih untuk mengadopsi anak anjing (kurang dari 6 bulan). 99,3% sadar akan penyalagunaan anjing dan kesejahteraannya.
Keyword: adopter, dog abuse, dog adoption

Judul: Risk Factor of Canine Aggression in Selangor Malaysia
Abstrak: Abnormal canine aggression is defined as normal aggressive behavior that becomes excessive or uncontrolled and is very dangerous and is unacceptable in our society. The purpose of this study is to perform a preliminary exploration on the risk factor of canine aggression in Selangor, Malaysia using a validated owner questionnaire. The research study design is a cross-sectional observational study using a questionnaire about the aggression behaviors of dogs of 100 dog owners who visited the Yap Animal Clinic over a period of time (From March 2020 to July 2020) about the aggression behaviors of their dogs. Descriptive statistics and preliminary analysis on owner’s (age and sex), dog’s (breed, age, sex, neuter status, size, housing) were calculated using Chi-Square. In this study, male owners were reported to have greater dog’s aggression compared to female owners. There were more female respondents (75%) to the questionnaire than males (25%). It is possible that this inequality in respondent gender resulted in a bias related to the recognition of, or admittance to, aggression in dogs. This study found outdoor dogs that were exclusively kept outside the home showed more aggression than indoor dogs that were kept just inside home. Outdoor keeping may provide more sources for dogs with no or less owner supervision, making them prone to higher ranking and dominance aggression. Dogs that were kept outside the house showed moderate to high levels of aggression., Perilaku agresif pada anjing yang abnormal didefinisikan sebagai perilaku agresif normal yang menjadi berlebihan atau tidak terkendali dan sangat berbahaya dan tidak dapat diterima di masyarakat. Tujuan dari penelitian ini adalah menentukan faktor risiko yang mempengaruhi agresi anjing di Selangor, Malaysia dengan menggunakan kuesioner kepada pemilik anjing yang telah divalidasi. Desain penelitian penelitian digunakan studi observasi cross-sectional dengan kuesioner tentang perilaku agresi anjing dari 100 pemilik anjing yang mengunjungi Klinik Hewan Yap, Selangor selama periode waktu Maret 2020 hingga Juli 2020. Statistik deskriptif dan preliminary analysis pada pemilik (usia dan jenis kelamin), anjing (ras, usia, jenis kelamin, status sterilisasi, ukuran, dan perkandangan) dihitung menggunakan Chi-Square. Hasil penelitian ini menunjukkan bahwa pemilik berjenis kelamin laki-laki dilaporkan memiliki anjing dengan tingkat agresi yang lebih tinggi dibandingkan dengan pemilik perempuan. Jumlah responden perempuan (75%) lebih banyak daripada laki-laki (25%). Terdapat kemungkinan bahwa ketidak setaraan dalam jumlah jenis kelamin responden ini menghasilkan bias yang terkait dengan pengakuan, atau penerimaan, agresi pada anjing. Studi ini juga menunjukkan bahwa anjing yang dipelihara di luar rumah memiliki tingkat agresi yang lebih tinggi daripada anjing yang dipelihara di dalam rumah. Anjing yang dipelihara di luar ruangan cenderung tidak mendapatkan atau kurang pengawasan pemilik. Hal tersebut menyebabkan anjing lebih rentan menunjukkan sikap dominasi disertai agresi. Anjing yang dipelihara di luar rumah menunjukkan tingkat agresi sedang hingga tinggi.
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Judul: Characterization and Expression of Tomato infectious chlorosis virus Coat Protein Gene in Escherichia coli
Abstrak: Tomato infectious chlorosis virus (TICV) is a member of Crinivirus genus. TICV infects tomato crop in Garut and Cipanas, West Java Indonesia. Tomato initially showed bright interveinal yellowing symptoms. The coat protein gene (CP-TICV) was amplified by RT-PCR from total RNA extracted from infected tomato leaves and the amplified fragment was cloned and completely sequenced. The fragment was subsequently subcloned into the pET-21b expression vector. The recombinant plasmid was transformed to Escherichia coli strain BL21(DE3)pLysS to express the coat protein. The coat protein fused to a 6xhistag, was purified by affinity chromatography using a NiNTA spin column. The identity of the purified protein was confirmed by SDS-PAGE. In this experiment, 792 bp of CP-TICV gene of TICV virus has been successfully cloned, sequenced, and expressed in E. coli. Based on nucleotide sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain strain and 99% identity to North America, France, and California strains and based on amino acid sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain, North America, France strains and 99.2% identity to California strain. CP-TICV showed over expressed in E. coli when it is induced with 1 mM IPTG and incubated at 37oC. Purified CP-TICV-Histag recombinants protein sized 29 kDa based on SDSPAGE analysis.
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