Judul: Identifikasi Keragaman Gen Miostatin pada Domba Lokal
Abstrak: Myostatin is a member of tranforming grown factor B superfamily, know as growth and differentiation factor, which is essensial for proper regulation of skeletal muscle mass in wide range vertebrate species. To pursue potential agricultural application of increasing muscle mass by inhibition of myostatin activities, the aim of this study was to identify the variants of myostatin gene in the Indonesian local sheeps. A total of 151 DNA samples belonging to eight different areas that are Bogor Ciomas (20), Bogor Jonggol (35), Margawati (18), Indramayu (30), Dinggala (24), MAdura (8), Sumbawa (8), and Rote (8) were analyzed with Polymerase Chain Reaction-Single Starnd Conformation Polymorphism. The Myostatin (GDF 8) amplified product was 688 bp(base pair)
Keyword: 

Judul: Identifikasi Keragaman Gen Calpastatin (CAST-NcoI) dengan Teknik PCR-RFLP pada Domba Lokal Indonesia
Abstrak: Domba lokal di Indonesia memiliki beberapa keunggulan, yaitu mampu bertahan pada iklim tropis, bersifat prolifik, tidak mengenal musim kawin, tahan terhadap beberapa macam penyakit dan parasit. Namun, produktivitas domba lokal masih cukup rendah terutama sifat pertumbuhan dan kualitas daging. Salah satu upaya seleksi dilakukan untuk meningkatkan produktivitas domba lokal yaitu berdasarkan pada penciri DNA yang dikenal dengan Marker Asissted Selection (MAS). Gen calpastatin merupakan gen yang berfungsi menghambat degradasi protein sel-sel otot. Pada mamalia, gen calpastatin diduga memiliki keterkaitan dalam sifat pertumbuhan otot dan keempukan daging. Tujuan dari penelitian ini adalah untuk mengidentifikasi keragaman gen calpastatin pada domba lokal . Sampel DNA yang digunakan dalam penelitian ini berjumlah 232 sampel, yang terdiri dari Domba Ekor Tipis (DET) Jonggol (38), DET Rote (25), domba Garut Ciomas (25), domba Garut Margawati (23), domba Garut Wanaraja (25), Domba Ekor Gemuk (DEG) Indramayu (25), DEG Madura (25), DEG Donggala (25), dan DEG Sumbawa (21). Amplifikasi gen calpastatin dilakukan dengan teknik PCR, sedangkan untuk menentukkan genotipenya dilakukan dengan teknik Restriction Fragment Length Polymorphism (RFLP) menggunakan enzim restriksi NcoI. Analisis data dilakukan dengan menghitung nilai frekuensi alel dan frekuensi genotipe.
Keyword: 

Judul: Pembuatan Film Tipis Cu2ZnSnS4 sebagai Lapisan Penyerap Cahaya pada Sel Surya Menggunakan Metode Elektrodeposisi
Abstrak: Thin film of Cu2ZnSnS4 (CZTS) is an alternative semiconductor used as light absorber layer on solar cell. CZTS can replace silicon that commonly used as commercial semiconductor. The advantage of using CZTS as absorber layer is due to the abundance of its chemical composer on earth, and its high value of absorption coefficient. The absorber layer was made by using electrodeposition method on ITO glass substrates. The films were deposited at room temperature using potentiostatic mode with a potential of –1.05 V for 30, 45, and 60 minutes. After that, the films were annealed at two different temperatures, 180 ºC and 500 °C, for 45 minutes in N2/H2S atmosphere. Based on X-ray diffraction analysis, the polycrystalline thin film obtained fall into kesterite crystal phase. This type of crystal was obtained when the CZTS was annealed at 500 °C in H2S/N2 atmosphere. The value of optical band gap energy for the CZTS thin film was 2.2 eV with the film thickness about 1.228–6.366 μm. The sample of CZTS with electrodeposition time of 45 minutes and annealing temperature of 500 °C showed the best result and in conformity with absorber layers criteria for solar cells.
Keyword: semiconductor, ITO glass, CZTS thin film, electrodeposition