Judul: The Role of Tengger Tribe Customary Law in the  Conservation of Natural Resources in the Bromo Tengger Semeru National Park
Abstrak: Kelestarian hutan salah satunya dipengaruhi oleh partisipasi masyarakat lokal. Kearifan lokal berupa hukum adat merupakan aturan hidup bagi masyarakat lokal yang bersangkutan sehingga harus menjadi pertimbangan dalam pengelolaan hutan, terutama dalam pengelolaan kawasan hutan konservasi di Indonesia. Penelitian ini bertujuan menilai hukum adat Suku Tengger di Desa Ngadas terkait pelestarian sumber daya alam kawasan Taman Nasional Bromo Tengger Semeru. Metode pengumpulan data yang digunakan yaitu observasi lapang, wawancara, dan pengisian kuesioner lalu dianalisis menggunakan deskriptif kualitatif. Hasil penelitian menunjukkan bahwa Suku Tengger memiliki hukum adat yang selaras dengan kebijakan nasional. Kepatuhan masyarakat terhadap hukum adat termasuk pada kategori patuh sehingga hukum adat masih terus berkelanjutan terutama terkait pelestarian sumber daya alam. Hukum adat juga memiliki kontribusi dalam hal konservasi tanah, air, tumbuhan, dan ekosistem hutan karena masyarakat masih mengimplementasikan hukum adat dengan baik dalam pemanfaatan sumber daya alam., Forest sustainability is influenced by the participation of local communities. Local wisdom in the form of customary law is a rule of life for the local community. It must be taken into consideration in forest management, especially in the management of conservation forest areas in Indonesia. This study aims to assess the customary law of the Tengger Tribe in Ngadas Village related to the preservation of natural resources in the Bromo Tengger Semeru National Park. Data collection methods used are field observations, interviews, also questionnaires and then analyzed with using descriptive qualitative. The results shows that the Tengger Tribe has customary laws that are in line with national policies. Community compliances with customary law is in the obedient category so that customary law is still sustainable, especially to the related preservation in natural resources. Customary law also has a great contribution in terms of conservation of soil, water, and forest ecosystem. The community still implements customary law well in the utilization of natural resources.
Keyword: Hukum adat, pelestarian, sumber daya alam, Suku Tengger, Taman Nasional Bromo Tengger Semeru

Judul: Etnobotani Masyarakat Tengger: Studi Kasus Desa Ngadas Wilayah Enclave Taman Nasional Bromo Tengger Semeru
Abstrak: Kawasan pelestarian alam di Jawa Timur salah satunya yaitu Taman Nasional Bromo Tengger Semeru (TNBTS). Penelitian bertujuan untuk melakukan studi etnobotani dalam rangka pelestarisan pengetahuan. Prosedur penelitian meliputi studi literatur, observasi lapang, wawancara, analisis vegetasi, dan analisis data. Hasil penelitian menunjukkan pemanfaatan tumbuhan oleh masyarakat Tengger Desa Ngadas sebanyak 69 jenis tumbuhan. Kelompok penggunaan terdiri dari tumbuhan pangan dan sayuran, tumbuhan obat, tumbuhan adat dan keagamaan, tumbuhan untuk kayu bakar, bahan bangunan, konservasi, dan pakan ternak. Indeks Nilai Penting tertinggi plot ladang untuk tingkat pertumbuhan pohon dimiliki oleh cemara gunung sebesar 229,56% pada plot hutan untuk tingkat pertumbuhan pohon dimiliki oleh kayu manisan sebesar 39,95%. Nilai Index of Cultural Significance tertinggi dimiliki oleh padi yang berasal dari luar Tengger sebesar 72. Indeks Nilai Penting cemara gunung di ladang sebesar 229,56% dan nilai Index of Cultural Significance sebesar 45. Hal ini menunjukkan pelestarian cemara gunung tinggi jika dilihat dari nilai INP meskipun pemanfaatan cemara gunung tergolong ke dalam kategori sangat tinggi.
Keyword: Ethnobotany, Ngadas, Tengger

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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