Judul: Kajian vaksinasi Infectious Bursal Disease (IBD) dengan uji serologi dan Imunohistokimia pada broiler
Abstrak: In Indonesia,Infectious Bursal Disease (IBD)has been an important poultry disease because of its immunosuppressive and mortality impacts.To prevent the disease, avariety ofvaccines containing different IBD virus strainsare currently available in the market. This study was aimed to compare IBD vaccination strategies using 3 different vaccines in broiler chicken followed by challenge using Indonesian field virus (Kediri strain from East Java, Indonesia). There were 8 broiler groups, group I as infection control (did not vaccine, but infected), and group NI as vaccine negative control (did not infected, and did not vaccine). Vaccine used in this study are vaccine strain W2512 (intermediate plus vaccine) which applied at hatchery, vaccine strain D78 (intermediate vaccine) which applied at 13 days and vaccine strain 228E (intermediate plus vaccine) which applied at 13 days. Evaluation antibody titer by using Enzyme Linked Immunosorbent Assay/ELISA method at 21 and 29 days, demonstrated that there were no significantly difference among all groups. Applied of vaccination made decreased in bursal index while infection of IBD will increasing in bursal index. Immunohistochemistry works showed that there were positive immunoreactivities results by using field virus primary antibody in both vaccinated groups and infected groups
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Judul: Kajian Serologis Dan Molekuler Ayam Pedaging Yang Divaksinasi Menggunakan Vaksin Infectious Bursal Disease (IBD) Berbagai Tingkat Dosis
Abstrak: Disease of Infectious bursal disease (IBD) is a viral disease that causes economic losses to the poultry industry as it causes high mortality and immunosuppression. IBD disease prevention efforts carried out by means of vaccination and biosecurity. IBD vaccination of day old security needs to be investigated its ability to prevent infection. This study aimed to observe the serological response and clinical condition based on examination of molecular actively vaccinated with IBD vaccine at different dose levels of daily life and challenged with virus field isolates. A total of 180 chickens were divided into four groups: K1 (full dose), K2 (½ Dose), K3 (dose ¼) and K4 (Dose 0). The vaccine used was a live IBD vaccine strains intermediate. Observations carried out on five chickens from each group that includes: antibody titers, the ratio bursa weight/bodyweight and pathology images. Presence of field virus infection and the linical condition due to infection challenge was observed by PCR. Observations made on days 1, 7, 28 and 42. Observations showed that the full dose vaccinated chickens started to show positive antibody formation against IBD at day 28 after vaccination, while in chickens vaccinated with a half and a quarter dose positive antibody titers 42 days post-vaccination. Clinical observations showed that vaccinated chickens various dose levels showed the same clinical symptoms than the control group. Similarly to the results of PCR observations it appears that the chickens from the vaccinated group of IBD viruses detected. The results of this study suggest that active IBD day old vaccination is not effective in preventing infection of the field.
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Judul: Cycle Hamilton pada Graf Lengkap, Graf Regular, dan Graf 2-Connected 4-Regular Berorder Kurang dari Sepuluh
Abstrak: Suatu graf disebut graf Hamilton apabila graf tersebut memuat cycle Hamilton, yaitu cycle yang melewati setiap vertex tepat satu kali. Menentukan keberadaan cycle Hamilton pada suatu graf dapat menggunakan syarat cukup yang berupa teorema-teorema yang telah diperkenalkan sejak tahun 1950-an. Beberapa syarat cukup yang dibahas dalam karya ilmiah ini adalah syarat cukup yang berhubungan dengan graf lengkap dan graf regular. Suatu graf lengkap berorder 𝑛������ ≥ 3 dan graf r-regular berorder 𝑛������ ≤ 2𝑟������ + 1 merupakan graf Hamilton, Karena kedua graf tersebut memuat cycle Hamilton. Selanjutnya, syarat cukup tersebut digunakan untuk menentukan keberadaan cycle Hamilton pada graf 2-connected 4- regular berorder kurang dari 10.
Keyword: graf Hamilton, graf regular, graf lengkap, graf 2-connected 4-regular