Judul: Profil Darah Rutin Tikus Sprague Dawley Pada Uji Toksisitas Subakut Ekstrak Kapang Endofit Daun Sirsak (Annona Muricata L.)
Abstrak: Ekstrak kapang endofit daun sirsak diketahui memiliki aktivitas sebagai obat kanker payudara. Uji toksisitas subakut perlu dilakukan untuk dapat menjadikannya sebagai obat. Ekstrak diberikan secara oral sebanyak 1 ml. Ekstrak diberikan dalam dosis 20 mg/kgBB, 200 mg/kgBB, dan 1000 mg/kgBB, 9 kali selama 28 hari. Uji darah rutin berfungsi sebagai penyaringan untuk mengetahui adanya kelainan proses fisiologi tubuh. Analisis darah rutin dilakukan pada eritrosit, leukosit, hemoglobin, hematokrit, dan diferensial leukosit. Hasil penelitian menunjukkan bahwa jumlah eritrosit dan leukosit menurun. Kadar hemoglobin normal yaitu 12.5-13 g/dl dan hematokrit normal, kecuali dosis 20 mg/kgBB yaitu 32%. Nilai diferensial leukosit untuk neutrofil meningkat hingga 21% dan nilai limfosit berada di rentang normal yaitu 79-85%. Uji analisis ANOVA menunjukkan hasil yang tidak berbeda nyata pada taraf keperayaan 5%.
Keyword: daun sirsak, darah, dosis, kapang endofit, subakut

Judul: Analisis Plateletcrit dan Red Blood Cell Distribution Width pada Uji Toksisitas Subkronis Ekstrak Kapang Endofit Daun Sirsak (Annona muricara L.)
Abstrak: Penelitian sebelumnya menemukan adanya aktivitas antikanker ekstrak kapang endofit daun sirsak (Annona muricata L.) terhadap sel kanker payudara MCF-7. Uji toksisitas subkronis perlu dilakukan sebelum dapat masuk ke fase uji klinis. Penelitian ini bertujuan mengukur nilai plateletcrit (PCT) dan red blood cell distribution width (RDW) pada tikus betina Sprague-Dawley yang diinduksi dengan variasi dosis ekstrak kapang endofit untuk melihat toksisitasnya. Tikus dibagi menjadi enam kelompok, yaitu kelompok normal, kelompok perlakuan dengan dosis 20, 80, dan 240 mg/kg bb, serta kelompok satelit dengan satelit normal dan satelit dosis 240 mg/kg bb. Kelompok satelit digunakan untuk melihat adanya efek tertunda. Hasil uji ANOVA menunjukkan tidak terdapat perbedaan nyata pada parameter PCT dan RDW antara kelompok normal dengan kelompok yang diinduksi sediaan uji. Data kelompok satelit juga tidak menunjukkan adanya perbedaan nyata. Simpulan dari penelitian ini adalah ekstrak etil asetat kapang endofit amat dikonsumsi selama 90 hari terhadap parameter RDW dan PCT., Previous studies showed that the endophytic mold extracted from soursop leaves (Annona muricata L.) has an anticancer ability against MCF-7 breast cancer cells. A subchronic toxicity test was needed before proceeding into the clinical trial. This study aimed to compare the value of plateletcrit (PCT) and red blood cell distribution width (RDW) on female Sprague-Dawley rats induced with endophytic mold extract with various doses to see its toxicity. The rats were grouped into six groups, the control group, groups that were given the extract with doses of 20, 80, and 240 mg/kg bw, as well as satellite groups that consist of normal satellite group and satellite group which was induced with 240 mg/kg bw dose of extract. The satellite group was used to show any delayed toxicity effect. ANOVA test for both parameters showed no significant difference between the control group and the group induced with endophytic mold extract. The satellite group also didn’t show any significant difference for both parameters. It can be concluded that the ethyl acetate extract of endophytic mold from soursop leaves is safe to be consumed for 90 days in regards to the PCT and RDW parameters.
Keyword: endophytic fungi, plateletcrit, red blood cell distribution width, toxicity

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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