Judul: Analisis Perubahan Penggunaan Lahan dan Perkembangan Wilayah pada Lingkungan Kota Depok
Abstrak: Kota Depok mengalami perkembangan wilayah dan urbanisasi yang dipengaruhi perluasan perkotaan Jakarta. Hal ini terlihat dari adanya perubahan penggunaan lahan pertanian ke non pertanian yang berdampak pada kualitas lingkungan. Penelitian ini bertujuan mengidentifikasi perubahan penggunaan lahan tahun 2011-2018, menganalisis kualitas lingkungan dengan Local Environmental Sustainability Index (LESI) dan menganalisis faktor-faktor yang mempengaruhi kualitas lingkungan di Kota Depok. Analisis perubahan penggunaan lahan dilakukan dengan overlay (SIG) peta penggunaan lahan tahun 2011 dan 2018 serta menggunakan tabulasi matriks perubahan lahan. Penetapan Indeks Kualitas lingkungan (LESI) didasarkan metode yang dikembangkan Pravitasari et al. (2016). Faktor-faktor yang mempengaruhi kualitas lingkungan wilayah dianalisis dengan regresi berganda dimana salah satu variabelnya adalah indeks perkembangan wilayah (IPW) tiap kelurahan dari hasil skalogram. Ruang terbuka hijau, permukiman dan jasa/industri mengalami peningkatan luas dari tahun 2011 ke 2018. Luas lahan sawah dan pertanian lahan kering mengalami penurunan. Pengelolaan urbanisasi yang disertai bertambahnya lahan terbangun dan ruang terbuka hijau (RTH) di Depok telah semakin berpengaruh signifikan meningkatkan kualitas lingkungan di Kota Depok., Urbanization in Jakarta and its surroundings has influenced the development of Depok City. An indication of the ongoing urbanization process is the change in land use from agriculture to non-agriculture. These conditions can affect the land quality and environment. This study aims to identify land use changes in 2011-2018, to analyze environmental quality with the Local Environmental Sustainability Index (LESI) and to analyze factors that affect environmental quality in Depok City. Land use change analysis was carried out by overlaying (GIS) land use maps from 2011 to 2018 and using a land change matrix tabulation. The factors that affect the quality of the regional environment were analyzed by multiple regression in which one of the independent variables was the regional development index (IPW) of each urban village (kelurahan) from the scalogram results. Green open space, settlements, and services/industries have increased in area from 2011 to 2018. On the other hand, the area of rice fields and dry land agriculture has decreased. The increase in built-up land in Depok City shows that the urbanization process is being/has occurred. Depok City. The management of green open space (RTH) has significantly improved the environment quality.
Keyword: Urbanisasi, Lahan terbangun, Ruang Terbuka Hijau, LESI

Judul: Trends in Land Cover Change and Sustainability Development of Depok City
Abstrak: Sebagai bagian dari wilayah metropolitan Jakarta atau Jabodetabek yang saat ini masih terus berkembang, Kota Depok banyak mengalami perubahan fisik secara pesat. Pembangunan suatu wilayah biasanya beriringan dengan proses perkembangan wilayah tersebut. Perkembangan suatu kota tidak dapat dipisahkan dari perencanaan dan keberlanjutannya. Maraknya perkembangan yang terjadi pada Kota Depok sejak tahun 2000 turut memberikan dampak pada perubahan penutupan lahan. Di satu sisi, berkembangnya Kota Depok dapat meningkatkan taraf perekonomian, namun seringkali masifnya pembangunan juga dapat menimbulkan penurunan kualitas lingkungan dan mengancam keberlanjutan pembangunan. Penelitian ini bertujuan untuk mengetahui tren perubahan dan prediksi tutupan lahan ke depan, serta keberlanjutan pembangunan di Kota Depok. Tren perubahan tutupan lahan di Kota Depok dilakukan dengan metode overlay pada software ArcGIS menggunakan data tutupan lahan tahun 2000, 2009, dan 2019 yang bersumber dari Kementrian Lingkungan Hidup dan Kehutanan (KLHK). Prediksi perubahan tutupan lahan tahun 2039 dianalisis menggunakan metode Land Change Modeler (LCM) dengan skenario business as usual (BAU). Sementara itu, status keberlanjutan pembangunan dianalisis dengan metode Multidimensional Scaling (MDS) Rapfish menggunakan data/atribut dari dimensi ekonomi, sosial, dan lingkungan. Hasil penelitian menunjukkan perubahan tutupan lahan Kota Depok pada tahun 2000 – 2019 didominasi oleh pertanian lahan kering menjadi permukiman. Pada tahun 2009 – 2019 Kota Depok didominasi oleh permukiman, sedangkan empat jenis tutupan lahan lain diantaranya, badan air dan pertanian lahan kering mengalami peningkatan, sedangkan pertanian lahan kering campur dan sawah mengalami penurunan luas. Beberapa lahan terbuka diproyeksikan akan mengalami penurunan luasan yang cukup signifikan pada tahun 2039, sedangkan permukiman akan terus mengalami peningkatan luas. Hasil analisis MDS Rapfish menunjukkan bahwa kecamatan- kecamatan di Kota Depok memiliki status keberlanjutan pembangunan yang berbeda-beda, baik dalam dimensi ekonomi, sosial, maupun lingkungan., As part of the Jakarta or Jabodetabek metropolitan area which is currently still growing, Depok City has undergone many rapid physical changes. The development of a region usually goes hand in hand with the process of developing the region. The development of a city cannot be separated from its planning and development. The many developments that have occurred in Depok City since 2000 have also had an impact on changes in land cover. On the other hand, the development of Depok City can increase the level of the economy, but often the quality of development can also cause environmental and development degradation. This study aims to determine the trend of changes and predictions of land cover in the future, as well as development in Depok City. The trend of land cover change in Depok City is carried out using the overlay method on ArcGIS software using land cover data for 2000, 2009, and 2019 sourced from the Ministry of Environment and Forestry (KLHK). The prediction of land cover change in 2039 was analyzed using the Land Change Modeler (LCM) method with a business as usual (BAU) scenario. Meanwhile, the development status was analyzed using the Rapfish Multidimensional Scaling (MDS) method using data on economic, social, and environmental dimensions. The results showed that land cover changes in Depok City in 2000 – 2009 were dominated by dry land into agriculture. In 2009 – 2019 Depok City was dominated by land
Keyword: built-up areas, development, Land Change Modeler, Multidimensional Scaling

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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