Judul: Potency of bacteria isolates from rumen buffaloes for enhancing forage digestion and performance of frisian holstein calves
Abstrak: Rumen bacteria plays an important role in digesting feed. The respective bacteria are adapted to low quality forage from agricultural by-product, especially those originated from rumen fluid of buffaloes. Study of innoculating buffalo rumen bacteria as probiotic into the rumen of Holstein Frisian calves have not been investigated so far. The aims of the present study were: (1) to isolate and characterize bacteria from buffalo, and (2) to evaluate the quality of isolates for enhancing forage digestion in Frisian Holstein calves. The study consisted of three studies: (1) Isolation, characterization, and genetic identification of buffalo rumen bacteria, (2) in vitro evaluation on various sources of forage and concentrates, and (3) in vivo evaluation into calves with different inoculation period. Design of the first study was based on a completely randomized factorial design with two factors: the type of bacteria and types of forage source, in which the diversity of bacteria were analyzed using NTSys 2.10 program. The second study was also based on a completely randomized factorial design, where the factors were various bacterial sources and feed types. The third study was consisted of two experiments, both were using completely randomized designs. The first experiment was to determine the effectiveness of bacteria consortium innoculation into Frisian Holstein calves with three calves received the respective inoculation (treatment group) and four calves without any inoculation (control group). The second experiment was todetermine the potency of bacteria consortium innoculation into Frisian Holstein calves during preweaning with three calves as treatment group and three calves as control. The results showed that: (1) buffalo rumen bacteria can grow well on forage substrates in which 14 isolates of bacteria possessed a high adaptability to fiber (high CMCase activity); these isolates could be clustered into six types which have similarity ≥ 51%. (2) Dry matter digestibility of concentrate were almost the same between the consortium of bacteria and buffalo rumen fluid. (3) buffalo rumen bacteria is a potential probiotic and could effectively be innoculated since preweaning period. (4) Inoculation of bacteria consortium increased feed intake and cobalt uptake on weaning period. (5) Inoculation of bacteria consortium did not cause any negative effects on ADG, physiological status and rumen fermentability.
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Judul: Peran Isolat Bakteri Pencerna Serat Dalam Pencernaan Nutrien Pada Pedet Lepas Sapih Yang Diberi Pakan Periode Pertumbuhan
Abstrak: In Indonesia, milk and beef production do not meet the needs. High calves mortality is one of the factors related to the problem. Reducing calf mortality and shortening weaning period are necessary to improve the population and farmers profit. The research was designed to evaluate the effect of inoculation of fiber degrading bacteria during pre-weaning period of dairy calves on nutrient intake and digestibility in post-weaning. The treatments were control and inoculation of bacteria isolates that applied in four and three Frisian Holstein calves, respectively. Both treatments were applied to the calf by a complete randomized design. Variables measured were nutrient intake, dry and organic matter digestibility, and the concentration of ruminal ammonia (NH3) and volatile fatty acids (VFA). Dry matter intake was determined from the beginning of the experimental period. Nutrient digestibility, ruminal NH3 and VFA concentration were determined four weeks after weaning. The data obtained were analyzed statistically using t-test method with α = 0.05. The results showed that the inoculation of bacteria isolates affected the concentration of ruminal VFA of weaned calves. Inoculation of bacteria isolates had no significant effect on nutrient intake, nutrient digestibility and concentrations of ruminal ammonia. It was concluded that inoculation of fiber degrading bacteria into the rumen of calves in milk feeding period improved nutrient fermentability but not improved nutrient intake and its digestibility in post-weaning period.
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Judul: Closed Knight’s Tour pada Papan Catur Berbentuk Persegi Panjang dengan Ukuran Maksimal 8 × 8
Abstrak: Closed Knight’s Tour merupakan langkah kuda yang mengunjungi setiap kotak pada papan catur tepat satu kali dengan langkah kuda berawal dan berakhir pada kotak yang sama. Graf yang terbentuk dari closed knight’s tour dinotasikan sebagai graf G(m,n), dengan m adalah banyaknya baris dan n adalah banyaknya kolom. Graf tersebut memuat Hamiltonian cycle. Sebuah graf terhubung G adalah graf bipartite jika dan hanya jika graf tersebut tidak memiliki cycle dengan panjang ganjil. Papan catur yang memenuhi syarat cukup untuk memiliki closed knight’s tour adalah papan catur berukuran 5 × 6, 5 × 8, 6 × 6, 6 × 7, 6 × 8, 7 × 8, dan 8 × 8.
Keyword: bipartite, closed knight’s tour, hamiltonian cycle, knight’s tour, teori graf