Judul: Antioxidant Capacity and Inhibition of Pancreatic Lipase Results from In vitro Fractionation of Citronella (Cymbopogon Nardus)
Abstrak: Obesitas memiliki faktor risiko yang tinggi dan dapat ditangani dengan menghambat enzim lipase pankreas. Obesitas memicu stres oksidatif yang dapat dicegah dengan antioksidan oleh senyawa fenolik dan flavonoid. Serai wangi (Cymbopogon nardus) belum diketahui potensi fraksinasinya sebagai inhibitor lipase pankreas secara in vitro. Penelitian ini bertujuan mencari fraksi terbaik pada kadar fenolik dan flavonoid total serta kapasitas antioksidan dan inhibisi lipase pankreas. Serai wangi diekstraksi dengan etanol 70% lalu dilanjutkan fraksinasi bertingkat dengan pelarut n-heksan, kloroform, etil asetat, dan air lalu dihitung rendemennya. Selanjutnya diuji kadar fenolik dan flavonoid total serta kapasitas antioksidan dari metode DPPH dan FRAP, lalu diuji daya inhibisi terhadap lipase pankreas. Hasil penelitian menunjukkan bahwa kadar fenolik total tertinggi terdapat pada fraksi air, kadar flavonoid total pada fraksi kloroform, kapasitas antioksidan metode DPPH dan FRAP pada fraksi etil asetat. Penghambatan lipase pankreas baik ekstrak kasar, fraksi, ataupun orlistat tidak berbeda nyata. Perbedaan kepolaran berpengaruh pada tiap uji karena afinitas yang berbeda terhadap sifat kepolaran, Obesity has a high risk factor and can be treated by inhibiting the pancreatic lipase enzyme. Obesity triggers oxidative stress which can be prevented by antioxidants by phenolic compounds and flavonoids. Citronella (Cymbopogon nardus) has no known fractionation potential as an inhibitor of pancreatic lipase in vitro. This research aimed to find the best fraction for total phenolic and flavonoid levels as well as antioxidant capacity and pancreatic lipase inhibition. Citronella was extracted with 70% ethanol then continued with multilevel fractionation with the solvent n-hexane, chloroform, ethyl acetate and water and then the yield was calculated. Next, total phenolic and flavonoid levels were tested as well as antioxidant capacity from the DPPH and FRAP methods, then the inhibitory power against pancreatic lipase was tested. The results showed that the highest total phenolic content was in the water fraction, total flavonoid content in the chloroform fraction, antioxidant capacity of the DPPH and FRAP methods in the ethyl acetate fraction. Inhibition of pancreatic lipase, whether crude extract, fraction or orlistat, was not significantly different. Differences in polarity affect each test because of different affinities for polar properties.
Keyword: in vitro, serai wangi, lipase pankreas, obesitas

Judul: Aktivitas Antioksidan dan Inhibisi Lipase Pankreas Hasil Fraksinasi Kayu Secang (Caesalpinia sappan L.) secara In vitro
Abstrak: Obesitas dapat menyebabkan stress oksidatif. Penelitian ini bertujuan untuk menguji total fenolik, total flavonoid, aktivitas antioksidan, dan aktivitas inhibisi lipase pankreas fraksi kayu secang (Caesalpinia sappan L.). Uji fenolik menggunakan metode Follin-Ciocalteu, uji flavonoid (metode AlCl3), sedangkan uji antioksidan (metode DPPH dan FRAP). Hasil penelitian menunjukkan bahwa fraksi kayu secang dengan pelarut polar (akuades) atau semipolar (etil asetat) memiliki fenolik, flavonoid, dan antioksidan yang tinggi yaitu sebesar 69 mg GAE/g CE; 143 mg QE/gr CE; dan 1254 mg TE/gr CE secara berturut-turut. Ekstrak kayu secang berpelarut nonpolar (N-heksana) lebih berpotensi sebagai inhibitor lipase pankreas karena memiliki nilai inhibisi sebesar 88,28%, lebih tinggi dibandingkan orlistat (82,34%). Hasil uji statistik menunjukkan bahwa total fenolik, flavonoid, dan aktivitas antioksidan dari kayu secang berbeda secara signifikan. Namun, aktivitas inhibisi lipase pankreas menunjukkan hasil sebaliknya. Berdasarkan penelitian ini, kayu secang berpotensi besar sebagai agen antioksidan dan inhibitor lipase pankreas., Obesity can cause oxidative stress. This study aimed to examine the total phenolics, total flavonoids, antioxidant activity, and pancreatic lipase inhibition activity of the secang wood fraction (Caesalpinia sappan L.). The phenolic test uses the Follin-Ciocalteu method, the flavonoid test (AlCl3 method), while the antioxidant test (DPPH and FRAP method). The research results showed that the secang wood fraction with polar (aquades) or semipolar (ethyl acetate) solvents had high phenolics, flavonoids and antioxidants, namely 69 mg GAE/g CE; 143 mg QE/gr CE; and 1254mg TE/gr CE respectively. Non-polar solvent secang wood extract (N-Hexane) has more potential as a pancreatic lipase inhibitor because it has an inhibition value of 88,28%, higher than orlistat (82,34%). The results of statistical tests showed that the total phenolic, flavonoid, and antioxidant activities of secang wood were significantly different. However, the inhibitory activity of pancreatic lipase showed the opposite results. Based on this research, secang wood has great potential as an antioxidant agent and pancreatic lipase inhibitor.
Keyword: antiobesitas, caesalpinia sappan, fraksinasi, inhibitor lipase pankreas

Judul: Implementation of Spatio-Temporal Change Query in Relational Database.
Abstrak: Geographic object changes in various views. Object changes are recorded using a model of static storage. No historical connection between old version data and new version data has been built. For that, a storage model of spatio-temporal queries that can analyze spatio-temporal changes has been developed. The object changes the geometry are grouped according to the taxonomy of Spatio-Temporal Pattern Change. This research has been able to apply the query patterns change including born, expand, merge, contract, split, normal and die. This query based on geometric (area) changes. The implementation of spatio-temporal change queries represents an object changes continuously with easy, change detection is done by comparing two versions of data. Keyword: spatio-temporal change query, spatio-temporal change pattern.
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