Judul: Analysis of ecological function of urban green open space for Bekasi's sustainability
Abstrak: Penelitian ini dilakukan untuk menganalisis kesesuaian fungsi ekologis dan persepsi masyarakat terhadap peran taman kota bagi keberlanjutan Kota Bekasi. Penelitian difokuskan pada tiga RTH yaitu Taman Kota Bekasi, Taman Pemerintah Kota Bekasi dan Hutan Kota Patriot Bina Bangsa yang berada di Kota Bekasi dengan luas masing-masing 1,19 Ha, 6,80 Ha dan 6,50 Ha. Penelitian ini dilakukan dengan tahapan persiapan, inventarisasi, analisis dan sintesis. Pengumpulan data dilakukan melalui observasi lapangan, wawancara dengan pengelola, studi literatur dan penyebaran kuesioner. Diperoleh taman-taman di Kota Bekasi sesuai terhadap fungsi ekologisnya karena dapat mereduksi kebisingan sebesar 16,03 dBA, menurunkan suhu sebesar 3,01°C, dan mengendalikan kelembaban sebesar 3,22%. Mayoritas responden sadar akan keberlanjutan dan peran mereka dalam mencapai keberlanjutan tersebut, juga sadar akan tindakannya dalam menjaga lingkungan dan berperan penting dalam keberlanjutan Kota Bekasi. Hal tersebut perlu mendapat dukungan pemerintah dalam pengembangan fasilitas dan utilitas serta sarana dan prasarana yang ada seperti taman kota yang ada di sekitar Kota Bekasi., This research aimed to analyze the ecological functions and public perceptions of urban parks in relation to the sustainability of Bekasi City. The research focused on three green spaces, namely Bekasi City Park, Bekasi City Government Park and Patriot Bina Bangsa Urban Forest in Bekasi City with an area of 1,19 Ha, 6,80 Ha and 6,50 Ha respectively. This research was conducted with the stages of preparation, inventory, analysis and synthesis. Data was collected through field observations, interviews with managers, literature studies and distributing questionnaires. The results show that the parks in Bekasi City fulfill their ecological functions as they can decrease noise levels by 16,03 dBA, lower temperature by 3,01°C, and control humidity by 3,22%. The majority of respondents are aware of sustainability and their contribution towards its attainment. Additionally, they acknowledge their environmental preservation efforts and pivotal role in the sustainability of Bekasi City. This needs government support in the development of facilities and utilities as well as existing facilities and infrastructure such as city parks around Bekasi City.
Keyword: Analisis, Keberlanjutan, Ruang terbuka hijau, green open space, sustainability

Judul: Analysis of City Parks Ecological Function for Tangerang City Sustainability
Abstrak: Taman kota sebagai salah satu jenis ruang terbuka hijau publik memiliki peran penting dalam meningkatkan kualitas lingkungan dan mendukung keberlanjutan perkotaan. Untuk mengetahui sejauh mana implementasi fungsi ekologis, diperlukan analisis terhadap fungsi-fungsi tersebut. Penelitian ini bertujuan untuk menganalisis kesesuaian fungsi ekologis dan persepsi dan preferensi masyarakat terhadap fungsi ekologis taman kota di Kota Tangerang. Penelitian dilakukan di tiga taman yang ada di Kota Tangerang yaitu Eco Park, Taman Pramuka, dan Taman Gajah Tunggal. Metodologi penelitian terdiri dari dua metode yaitu analisis fungsi ekologis dan analisis persepsi dan preferensi masyarakat di masing-masing taman. Fungsi ekologis dianalisis dengan membandingkan kondisi eksisting dengan kriteria standar, sedangkan analisis sosial dilakukan melalui pengumpulan data menggunakan kuesioner. Hasil analisis ekologi menunjukkan bahwa Eco Park unggul dalam fungsi modifikasi suhu. Taman Pramuka memiliki vegetasi yang berfungsi baik sebagai penahan angin dan penahan erosi. Taman Gajah Tunggal memiliki fungsi peredam kebisingan terbaik. Melalui penyebaran kuisioner diketahui bahwa mayoritas masyarakat merasa puas dengan kondisi ketiga taman tersebut., Urban city parks, as a type of public green open space, play an important role in improving the quality of the urban environment and supporting urban sustainability. To determine the extent of ecological function implementation, an analysis of these aspects is necessary. This research aims to analyze the suitability of ecological functions and examine public perceptions and preferences regarding the ecological functions of city parks in Tangerang City. The study was conducted in three parks in Tangerang City, namely Eco Park, Pramuka Park, and Gajah Tunggal Park. The research methodology consists of two methods: ecological function analysis and analysis of public perceptions and preferences in each park. Ecological functions are analyzed by comparing the existing conditions with standard criteria, while social analysis is conducted through data collection using questionnaires. The results of the ecological analysis show that Eco Park excels in temperature modification function. Pramuka Park has vegetation that adequately serves as windbreak and erosion control. Gajah Tunggal Park performs the best in noise reduction function. Through the distribution of questionnaires, it was found that the majority of the community is satisfied with the conditions of all three parks.
Keyword: city park, ecological function, green open space, urban sustainability

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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