Judul: Gaya Pengasuhan Ibu dan Interaksi Saudara Kandung pada Remaja berdasarkan Status Pekerjaan Ibu
Abstrak: Adolescents are very susceptible to encountering conflict everyday, one of which is the sibling relationship. Mother’s parenting could help adolescents handle it to be better. This research aims to know the influence of mother’s parenting style to sibling relationship based mother’s working status. The sample in this study are families who have more than one child and one of them is teenage aged 12-15 years old who lived in Kebon Pedes and Kedung Badak Village, Tanah Sareal, Bogor City. Sample taken by purposive sampling in 60 families consisting of 30 working mother families and 30 nonworking mother families that were interviewed with questionnaire. The results showed that there was no difference in mother’s parenting style and siblings relationship on working and nonworking mother families. Mother's working status and adolescent’s allowance influence on conflict/rivalry dimension in sibling relationship. Warmth/closseness dimension in sibling relationship affected by the high education of mother and the low authoritarian parenting style.
Keyword: working mothers., sibling rivalry, sibling relationship, non-working mothers, mother’s parenting

Judul: Gaya Pengasuhan Ibu, Interaksi Saudara Kandung, dan Kemandirian pada Remaja Anak Sulung Perempuan
Abstrak: Mencapai kemandirian merupakan salah satu tugas perkembangan yang harus dilakukan oleh remaja. Penelitian ini bertujuan untuk menganalisis hubungan gaya pengasuhan ibu (Parental Acceptance-Rejection), interaksi saudara kandung dan kemandirian pada remaja anak sulung perempuan. Penarikan contoh dilakukan secara purposive pada 60 remaja perempuan yang berusia 13-16 tahun di SMPN 2 Dramaga, Bogor. Contoh dalam penelitian ini dibedakan berdasarkan jenis kelamin adik yaitu remaja yang memiliki adik laki-laki sebanyak 30 orang dan adik perempuan sebanyak 30 orang. Hasil menunjukkan bahwa remaja mempersepsikan ibu melakukan pengasuhan penerimaan, interaksi kekuasaan saudara kandung memiliki indeks lebih tinggi daripada dimensi lain, dan kemandirian nilai memiliki indeks tinggi. Semakin tingginya penerimaan ibu akan meningkatkan kehangatan saudara kandung dan tingginya pengabaian ibu akan menurunkan kemandirian emosional pada remaja dengan adik perempuan. Pada remaja dengan adik laki-laki, penerimaan ibu akan meningkatkan persaingan dan interaksi kehangatan saudara kandung akan meningkatkan kemandirian emosional.
Keyword: anak sulung, gaya pengasuhan ibu (Parental Acceptence-Rejection), interaksi saudara kandung, kemandirian, remaja perempuan

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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