Judul: Perbandingan CHAID, CART, dan Random Forest dalam Mengklasifikasikan Anemia dan Non-anemia pada Remaja Putri di Indonesia
Abstrak: Kehidupan sehat dan sejahtera (good health and well-being) merupakan indikator ketiga Suistainable Development Goals (SDGs) yang ingin dicapai untuk mewujudkan negara yang berkualitas. Masalah kesehatan yang sering dijumpai adalah anemia, terutama dikalangan wanita. Anemia merupakan keadaan jumlah sel darah merah atau konsentrasi Hemoglobin (Hb) menurun dalam tubuh dan jumlahnya kurang dari normal. Kriteria anemia pada perempuan tidak hamil dengan usia 5 – 11 tahun memiliki kadar hemoglobin < 11,5 g/dL, kriteria anemia pada perempuan tidak hamil dengan usia ≥ 12 tahun memiliki kadar hemoglobin < 12,0 g/dL, sedangkan kriteria pada ibu hamil memiliki kadar hemoglobin < 11,0 g/dL.Pertumbuhan dan kematangan seksual menyebabkan remaja putri rentan terkena anemia yang dapat mempengaruhi kecerdasan serta daya tangkap. Penelitian dalam mencari tahu faktor yang mempengaruhi anemia pada remaja putri perlu dilakukan. CHAID, CART dan random forest merupakan metode pohon keputusan yang dapat memberikan informasi faktor apa saja yang mempengaruhi anemia pada remaja putri. Penelitian ini bertujuan untuk membandingkan metode klasifikasi CHAID, CART dan random forest berdasarkan peubah penciri dalam pengelompokkan anemia dan mengidentifikasi penciri anemia berdasarkan hasil ketiga metode klasifikasi terbaik. Hasil analisis menunjukkan bahwa terdapat 2 peubah penjelas yang sama dalam mengkategorisasikan anemia dan non-anemia pada metode CHAID dan CART yaitu status menstruasi dan usia kehamilan. Metode random forest, status gizi merupakan peubah yang berperan penting dalam memisahkan anemia dan non anemia. Meskipun random forest mempunyai nilai akurasi yang cenderung sama dengan metode CHAID dan CART dalam pengelompokkan remaja putri anemia dan non-anemia, namun metode CHAID dan CART dapat lebih jelas menunjukkan kategori peubahnya, sedangkan metode random forest tidak dapat menunjukkan kategori dari masing-masing peubah tersebut. Kata kunci : anemia, CART, CHAID, random forest
Keyword: anemia, CART, CHAID, random forest

Judul: Identifikasi Faktor-Faktor yang Mempengaruhi Status Anemia pada Ibu Hamil Menggunakan Metode CHAID dan Random Forest
Abstrak: Maternal anemia is one of problem in society. The high rates of maternal anemia in line with lack of awareness to iron consumption. The solution for this problem is classifiying the factors associated with maternal anemia status. CHAID method (Chisquare Automatic Interaction Detection) is one of classification method which type categorical response variable. In this study, CHAID method applied to eight explanatory variable with repetition three times so that the resulting classification tree is different. Differences in classification result with the CHAID method lead to instability identifier variable therefore proceed with the random forest method to see identifier variable that stable. Random forest produced three explanatory variables sequentially stable are upper arm circumference (lila), frequency of prenatal care (anc), and maternal age clinical. Identifier variable be produced random forest method applied to the CHAID method to see its classification. CHAID method with random forest criteria produce that maternal anemia criteria if maternal upper arm circumference (lila) less than 23.5 cm and frequency of prenatal care (anc) less than four times, where as maternal normal if maternal upper arm circumference (lila) more than 23.5 cm and frequency of prenatal care (anc) more than one.
Keyword: anemia, maternal, CHAID, random forest

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.