Judul: Desain ekologis Taman Sempur Kota Bogor berbasis preferensi masyarakat
Abstrak: Perkembangan Kota Bogor dengan pertumbuhan populasi dan permbangunan infrastruktur yang pesat menjadikan kota sebagai pusat aktivitas. Kebutuhan ruang publik pun menjadi sangat penting untuk mendukung aktivitas. Kenyataannya, kualitas taman tidak diimbangi fungsi ekologi tapak dan lebih berorentasi pada estetika dan sosial sehingga timbul permasalahan lingkungan. Konsep ecological design diperlukan untuk mengatasi permasalahan tersebut. Taman Sempur merupakan taman kota dengan luas 3,5 ha yang strategis dan potensial untuk dikembangkan. Tujuannya adalah untuk membentuk konsep ekologis dalam memenuhi kebutuhan masyarakat dan linkungan berdasarkan preferensi masyarakat. Konsep dasar yang diterapkan adalah urban environmental park dengan fokus pada penyediaan ruang, aktivitas dan perlindungan kawasan terutama pada kawasan riparian yang penting untuk direstorasi. Pertimbangan juga ditujukan pada peningkatan keanekaragaman hayati, penggunaan energi dan sumber daya, pengelolaan air, pengaturan limbah dan kenyamanan. Hasil pengembangan desain diwujudkan dalam pembagian ruang utama yaitu ruang penerimaan, ruang olahraga, ruang konservasi, ruang rekreasi dan lapangan serbaguna.
Keyword: Ecological design, Preference, City Park

Judul: Ecological Analysis Of City Parks For Sustainability In Bogor City.
Abstrak: Taman kota sebagai salah satu jenis ruang terbuka hijau publik memiliki peran penting dalam meningkatkan kualitas lingkungan perkotaan dan mendukung keberlanjutan kota. Untuk mengetahui sejauh mana pelaksanaan fungsi ekologi dan persepsi serta preferensi masyarakat perlu dilakukan evaluasi secara menyeluruh terhadap aspek-aspek tersebut. Penelitian ini bertujuan untuk mengidentifikasi vegetasi, menganalisis kesesuaian fungsi ekologis, dan menganalisis persepsi pengguna dan preferensi terhadap lanskap di setiap taman untuk kelestarian Kota Bogor. Penelitian ini berlokasi di tiga taman di Kota Bogor, yaitu Taman Heulang, Taman Sempur, dan Alun-alun Kota Bogor. Metode penelitian ini dibagi menjadi dua metode yaitu analisis fungsi ekologis dan analisis persepsi dan preferensi pengunjung pada masing-masing taman. Fungsi ekologis akan dianalisis dengan membandingkan kondisi eksisting dengan kriteria baku fungsi ekologis, sedangkan analisis persepsi dan preferensi masyarakat mengenai ekologis taman akan dianalisis dengan pengumpulan data melalui kuesioner. Hasil evaluasi ini menunjukkan bahwa Taman Heulang merupakan taman terbaik dalam hal pengurangan kebisingan, modifikasi temperatur (teduh) dan fungsi penahan angin. Taman Sempur memiliki vegetasi yang cukup baik dalam memenuhi fungsinya sebagai pengubah suhu (teduh) dan pengatur kelembaban. Alun-alun Kota Bogor merupakan taman terbaik dalam hal fungsi pengatur kelembaban udara dan cukup baik dalam fungsi penahan angin dan pengurangan kebisingan., City parks as a type of public green open space have an important role in improving the quality of the urban environment and supporting city sustainability. To find out the extent of the implementation of ecological functions and the perceptions and preferences of the community, it is necessary to carry out a comprehensive evaluation of these aspects. This research aims to identify vegetation, analyze the suitability of ecological functions, and analyze user perceptions and preferences for the landscape in each park for the sustainability of Bogor City. This research was located in three parks in Bogor City, namely Heulang Park, Sempur Park, and Bogor City Square. This research method is divided into two methods, namely ecological function analysis and analysis of visitor perceptions and preferences in each park. Ecological function will be analyzed by comparing existing conditions with standard criteria for ecological function, while analysis of community perceptions and preferences regarding park ecology will be analyzed by collecting data through questionnaires. The results of this evaluation show that Heulang Park is the best park in terms of noise reduction, temperature modification (shade) and wind barrier function. Sempur Park has vegetation that is quite good at fulfilling its function as a temperature changer (shade) and humidity regulator. Bogor City Square is the best park in terms of air humidity control function and quite good in terms of wind barrier and noise reduction functions.
Keyword: Analysis, City Park, Ecological Function, Green Open Space, Perception and Preference

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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