Judul: Abundance of Soil Macrofauna on Several Land Covers in Balangan Regency, South Kalimantan Province
Abstrak: Keberadaan makrofauna tanah memiliki peran penting dalam mempertahankan dan mengembalikan kesuburan tanah. Penelitian ini bertujuan menganalisis kelimpahan makrofauna tanah dan pengaruh jenis vegetasi, serta lingkungan terhadap kelimpahan makrofauna tanah pada beberapa tutupan lahan di Kabupaten Balangan, Kalimantan Selatan. Pembuatan petak dengan ukuran 20 m × 20 m secara purposive sampling. Analisis data dilakukan dengan uji Beda Nyata Terkecil (BNT) dan uji korelasi Pearson. Kelimpahan makrofauna tanah secara keseluruhan terdiri atas 15 ordo dan 27 famili dengan total individu sebanyak 370 individu/m². Hasil uji BNT menunjukkan kelimpahan makrofauna tanah berbeda nyata di hutan sekunder dan lahan kosong, sedangkan kelimpahan makrofauna tanah tidak berbeda nyata antara hutan meranti dan kebun karet. Berdasarkan uji korelasi Pearson, faktor klimatis kelembapan tanah memiliki hubungan positif yang sangat kuat terhadap kelimpahan makrofauna tanah, serta faktor edafis seperti porositas, C-organik, respirasi, berat basah, berat kering, dan kadar air juga memiliki hubungan positif yang sangat kuat terhadap kelimpahan makrofauna tanah., The existence of soil macrofauna has an important role in maintaining and restoring soil fertility. This study aimed to analyze the abundance of soil macrofauna and the influence of vegetation type, as well as the environment on the abundance of soil macrofauna in several land covers in Balangan Regency, South Kalimantan. Plots measuring 20 m × 20 m were made using purposive sampling. Data analysis used the Least Significant Difference (LSD) test and Pearson correlation test. The overall abundance of soil macrofauna consists of 15 orders and 27 families with a total of 370 individuals/m². The BNT test results showed that the abundance of soil macrofauna was significantly different in secondary forests and empty land, while the abundance of soil macrofauna was not significantly different between meranti forests and rubber plantations. Based on the Pearson correlation test, the climatic factor of soil moisture has a very strong positive relationship with the abundance of soil macrofauna, and edaphic factors such as porosity, C-organic, respiration, wet weight, dry weight, and moisture content also have a very strong positive relationship with the abundance of soil macrofauna.
Keyword: climatic, edaphic, formicidae, secondary forest, vegetation

Judul: Macrofauna Soil Diversity in Land Cover of Revegetation Areas at PT. Bukit Asam
Abstrak: Makrofauna tanah merupakan salah satu bioindikator dalam menganalisis perbaikan lahan pascatambang. Penelitian ini diharapkan mampu memberikan informasi mengenai pengaruh tutupan lahan terhadap makrofauna tanah di lahan revegetasi PT Bukit Asam. Penelitian ini dilaksanakan pada empat jenis lahan, yaitu lahan hutan merbau, hutan angsana, hutan sekunder, dan lahan kosong. Hasil uji Beda Nyata Terkecil (BNT) menunjukkan bahwa hutan merbau memiliki kelimpahan fauna tertinggi yaitu 79 individu/m2 dan lahan kosong memiliki kelimpahan fauna terendah yaitu 0 individu/m2. Hutan merbau terbukti berbeda nyata dengan lahan kosong pada selang 95%, sedangkan hutan angsana dan hutan sekunder tidak berbeda nyata. Kerapatan vegetasi yang paling rapat memiliki pengaruh terhadap jumlah fauna tanah yang ditemukan. Parameter klimatis yang memiliki tingkat korelasi sangat kuat dan kuat adalah intensitas cahaya dan suhu lingkungan. Parameter edafis yang memiliki tingkat korelasi sangat kuat dan kuat adalah bulk density, porositas, pH, C-Organik, dan respirasi tanah., Soil macrofauna is one of the bioindicators in analyzing post-mining land improvement. This research is expected to provide information on the influence of land cover types on macrofauna abundaced in the reforested land of PT Bukit Asam. This study was conducted on four types of land: merbau forest land, angsana forest land, secondary forest land, and vacant land. The results of the BNT test showed that merbau forest had the highest fauna abundance, with 79 individuals/m2, while vacant land had the lowest fauna abundance, with 0 individuals/m2. Merbau forest was significantly different from vacant land at the 95% confidence interval, while angsana forest and secondary forest did not show significant differences. The densest vegetation density has an influence on the number of soil fauna found. Climatic parameters with very strong and strong correlation levels are light intensity and environmental temperature. Edaphic parameters with very strong and strong correlation levels are bulk density, porosity, pH, Organic Carbon, and soil respiration.
Keyword: edafis, klimatis, lahan pascatambang, vegetasi

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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