Judul: Effect of Nitrogen and Phospor Fertilization on Chlorophyll and Nitrogen and Phospor Content in Transgenic Sugarcane of IPB 1
Abstrak: Penurunan produksi tanaman tebu terus terjadi, sementara itu kebutuhan gula di Indonesia semakin meningkat. IPB sebagai salah satu satu institusi yang bergerak dan fokus dalam bidang pertanian turut melakukan upaya-upaya dalam membantu program swasembada gula di Indonesia. Salah satunya adalah dengan melakukan penelitian tanaman tebu transgenik. Tebu transgenik ini adalah tebu hasil rekayasa genetika yaitu dengan menyisipkan gen fitase untuk menghemat pemupukan. Terkait dengan upaya-upaya tersebut dilakukan uji kandungan klorofil dan hara N dan P pada tanaman tebu transgenik IPB 1 untuk melihat lebih jauh kemampuan tebu transgenik IPB 1 ini dalam menghemat pemupukan. Penelitian dilakukan di Kebun Percobaan PG Djatiroto, PT Perkebunan Nusantara XI (PTPN XI), Kabupaten Lumajang, Jawa Timur, Laboratorium Indonesian Center for Biodiversity and Biotechnology (ICBB), Laboratorium Bioteknologi Tanah dan Laboratorium Kimia dan Kesuburan Tanah Departemen Ilmu Tanah dan Sumberdaya Lahan. Sebanyak 23 klon tebu transgenik IPB 1 dan 1 klon tebu isogenik PS 851 ditanam dengan empat perlakuan yaitu a (pemupukan N 50% dan P 50%), b (pemupukan N 100% dan P 50%), c (pemupukan N 50% dan P 100%), d (pemupukan N 100% dan P 100%) dan tiga kali ulangan dalam petakan seluas 10.000 m2. Sampel tanaman untuk analisis klorofil dan kandungan hara N dan P diambil dua kali, yaitu saat tebu berumur 6 bulan dan 9 bulan. Analisis kandungan klorofil dilakukan dengan dengan metode Wintermans and De Mots sedangkan kandungan hara N dan P dengan metode pengabuan basah. Skoring pada keragaan tanaman tebu juga dilakukan untuk mencari tujuh klon tebu terbaik. Hasil penelitian menunjukkan bahwa respon pemupukan dengan dosis N 50% dan P 50% pada tebu transgenik IPB 1 memiliki kandungan klorofil, dan hara N dan P yang hampir sama baik dengan pemupukan N 100% dan P 100%. Sebaliknya pada tebu isogenik PS 851 memiliki kecenderungan kandungan klorofil dan N dan P yang tinggi seiring dengan meningkatnya dosis pemupukan. Berdasarkan skoring keragaan dan hasil diperoleh tujuh klon terbaik tebu transgenik IPB 1. Tujuh klon tebu transgenik IPB 1 adalah IPB 1-1, IPB 1-3, IPB 1-6, IPB 1-46, IPB 1-52, IPB 56, dan IPB 1-59.
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Judul: Pengaruh Pemupukan N dan P Terhadap Keragaan dan Hasil Tebu Transgenik IPB 1 di PG Djatiroto, Jawa Timur
Abstrak: One approach to improve sugar production and saving of fertilizer is develoving genetically modified sugarcane plants. The transgenic sugarcane IPB 1 contains bacterial phytase gene that can alter phytic acid which is a form of organic-P to become inorganic-P, so it can be used by plants. Phytase will also increase the availability of other mineral nutrients in plant tissues such as Mg2+, Ca2+, and Fe2+. The nutrients are utilized by plant for synthesis of chlorophyll and inturn will increase photosynthesis and the yield of sugarcane. The research was aimed study to (1) the effectivity of N and P fertilization on transgenic sugarcane IPB 1 by observing growth and production of sugarcane, and (2) to select seven best clones by scoring the growth and production of sugarcane. The research was conducted at PG Djatiroto Experiment Station, Lumajang, East Java. Total of 23 clones of transgenic sugarcane IPB 1 and 1 clone of isogenic sugarcane PS 851 were treated with four fertilization, i.e.: (a) 50% N and 50% P, (b) 100% N and 50% P, (c) 50% N and 100% P, and (d) 100% N and 100% P, where 100% N and 100% P, respectively, equals to 800 kg ZA/ha and 200 kg SP-36/ha. 800 kg ZA/ha and 200 kg SP-36/ha together with 100 kg KCl/ha are the recommended fertilization rate for the area. Growth parameters abserved were number of stalk, stalk height, and stalk diameter. Measurement of the growth parameters were conducted twice, i.e. at the ages of 6 and 9 months (Januari and April 2011). Production parameters, namely yield, sugar content, and sugar production were determined after sugarcane harvesting at the age of 12 months. Some data were analyzed using Statistical Analysis Software (SAS) at α value of 0,05. The results showed that the transgenic sugarcane IPB 1 can effectively utilized fertilizers as indicated by insignificant effect of fertilization treatment. The best growth and production were resulted by 50% N and 50% P fertilization. Seven best clones at this rate of fertilizaation are transgenic sugarcane IPB 1-1, IPB 1-3, IPB 1-6, IPB 1-7, IPB 1-46, IPB 1-52, and IPB 1-56 the seven best sugarcane clones.
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Judul: Kekonsistenan Hamiltonian pada Gerak Gelombang Interfacial
Abstrak: The interfacial wave is an internal wave that occurs at boundary of two layers fluid. It appears because there is difference of mass density between the two layers. The objective of this thesis is to formulate the motion of internal wave with flat base and flat as well as free upper bound surface. The motion equation obtained is a Hamiltonian system. The Hamiltonian in this system is expressed by variable of wave deviation and horizontal component of potential velocity. Under assumption that interfacial wave moves only in one direction, it can be shown that the Hamiltonian depends on the solution of the wave motion equation. The coefficients of the equation depend on the physical characteristics of the two layers of fluid, i.e. thickness and mass density. The numerical results show that Hamiltonian in the system is constant. It is consistent with Hamiltonian characteristic, which does not depend on time.
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