Judul: Pengetahuan dan Sikap Gizi Ibu dengan Praktek Konsumsi Susu Anak Sekolah TK
Abstrak: Thisstudy is aimed to analyze correlation between mother’s nutritional knowledge and attitude and milk consumption of pre-school children’s (PSC). A cross sectional study design was applied among 136 PSC aged 4-5 yrs old in the two favorite kindergartens of Bogor City - Aliya and Mexindo. The mothers f the PSC filled in a questionnaire consist of sociodemographic characteristics, children’s milk consumption, and a nutritional knowledge-and-attitude. Milk consumsption of PSC was measured by applying a 30 days semi-FFQ method . The results show that most of mother had good nutritional knowledge and attitiude. More than 90% of them agree that drinking milk is a good habit for children. The mother’s nutritional attitudes showed significant positive correlation with children’s milk drinking habit (r = 0.31). No relation was found between nutritional knowledge with attitude and milk consumption
Keyword: pre-school children., milk consumption, nutritional attitude, nutritional knowledge

Judul: Hubungan pengetahuan gizi ibu dengan konsumsi buah dan sayur serta status gizi anak pra sekolah di TK Salman Jakarta
Abstrak: Tujuan dari penelitian ini adalah untuk mengetahui hubungan antara pengetahuan gizi ibu mengenai konsumsi buah dan sayur dan status gizi anak usia pra sekolah di TK Salman Jakarta. Sampel dalam penelitian ini berjumlah 43 orang yang dipilih berdasarkan stratifikasi metode acak. Pengumpulan data dilakukan dengan wawancara menggunakan kuesioner. milik ibu pengetahuan gizi diukur melalui kuesioner. Buah-buahan dan sayur-sayuran konsumsi diukur dengan catatan makanan. Konsumsi buah-buahan dan sayur-sayuran kebiasaan diukur dengan Food Frekwensi Questionnaire (FFQ). Status gizi dari anak usia pra sekolah diukur dengan metode antropometri dengan indeks BB/U, TB/U, dan IMT/U. Analisis data pengetahuan gizi ibu, konsumsi buah-buahan dan sayuran, dan status gizi menggunakan statistik deskriptif dan spearman korelasi. Hasil penelitian menunjukkan bahwa secara umum pengetahuan gizi ibu sedang 53,5%. Sampel konsumsi buah dan sayur pun masih kurang dari porsi yang dianjurkan, 3 porsi buah-buahan (300 g) dan 2 porsi sayuran (200 gram). Buah-buahan terbanyak dikonsumsi dengan frekuensi 7-14 kali kali/minggu berwarna oranye (27,9%). Sayuran yang paling banyak dikonsumsi adalah frekuensi 7-14 kali/minggu adalah wortel (23,3%). Status gizi berdasarkan indeks BB/U secara umum baik (72,1%), berdasarkan indeks TB/U secara umum baik normal (90,7%), dan berdasarkan IMT/U secara umum normal (69,8%). Hasil dari korelasi spearman menunjukkan tidak ada hubungan yang signifikan antar pengetahuan gizi ibu dengan konsumsi buah dan sayur (p>0,05) dan status gizi anak usia pra sekolah (p>0,05). Tidak ada yang signifikan hubungan antara konsumsi buah-buahan dan sayuran dengan status gizi pra anak sekolah (p>0,05).
Keyword: Nutritional knowledge, Fruits consumption, Vegetables consumption, Nutritional status, Pre school children

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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