Judul: Analisis Strategi Daya Saing Dan Daya Tarik Pasar Tradisional (Studi Kasus Pasar Johar Di Kota Semarang Provinsi Jawa Tengah)
Abstrak: Keberadaan Pasar Johar di Kota Semarang tidak lagi menjadi primadona masyarakat semarang dalam melakukan transaksi perdagangan, salah satu penyebab sepinya Pasar Johar adalah karena adanya Toko Modern seperti Departemen Store, Supermarket, Hypermarket dan Minimarket yang ada di Kota Semarang. Pedagang tradisional yang terkena imbas langsung dari keberadaan Toko Modern yang menjual produk yang sama dengan yang dijual di Pasar Johar. Meskipun demikian, pedagang yang menjual makanan segar (daging, ayam, ikan, sayur-sayuran, buah-buahan, dan lain-lain) masih bisa bersaing dengan Toko Modern mengingat banyak pembeli masih memilih untuk pergi ke pasar tradisional untuk membeli produk tersebut. Penelitian ini bertujuan untuk: mengidentifikasi penyebab turunnya minat masyarakat terhadap Pasar Johar, dengan menggunakan analisis TEMPLES untuk meneliti aspek eksternal lingkungan Pasar Johar, metode GE 9 Cell untuk memetakan Pasar Johar berdasarkan aspek daya tarik dan daya saing Pasar Johar dan analisis Blue Ocean Strategy untuk melihat aspek kompetisi Pasar Johar sehingga dapat merumuskan strategi baru Pasar Johar dengan menggunakan analisis lingkungan (TEMPLES) dan kompetisi Pasar Johar (Blue Ocean Strategy). Dari hasil analisis didapatkan 12 faktor yang berpengaruh terhadap daya saing dan daya tarik dari Pasar Johar: Kekuatan Manajemen, Permasalahan/isu Lingkungan, Citra (Pasar), Akses jaringan distribusi, Dukungan Pemasok, Trend Harga, Industri Pesaing, Kapasitas (Daya tampung/Penataan), Kekuatan Keuangan, Kebijakan/Regulasi, Loyalitas Pelanggan, dan Kualitas. Penyebab turunnya minat masyarakat terhadap Pasar Johar disebabkan karena faktor ketidaknyaman. Jumlah pedagang yang melebihi kapasitas juga mempengaruhi faktor lingkungan, sehingga pedagang menempati areal yang tidak diperuntukkan untuk berjualan. Dalam melakukan penataan baik fisik (bangunan pasar) maupun non fisik (manajemen pengelolaan pasar) dapat dilakukan melalui kerjasama antara pemerintah daerah dengan swasta. Selain itu, dalam rangka pengembangan Pasar Johar kedepan juga direkomendasikan menjadi Heritage Johar Market yaitu salah satu objek pariwisata Cagar Budaya, sehingga keberadaan bangunan Pasar Johar tetap lestari dan dapat dipertahankan sesuai dengan keaslian dari arsitekturnya.
Keyword: Attractiveness and Competitiveness, Blue Ocean Strategy, GE 9 CELL, Modern Market, Traditional Market

Judul: Strategi Peningkatan Posisi Tawar Pasar Tradisional terhadap Pedagang di Kota Bogor
Abstrak: Traditional market in Bogor Regency is a place to meet transaction between producers and consumers which has the strategic value for the developing people’s economic growth. But, the embeded problem, the traditional market is still low competitiveness if it is compared to the modern market (hyper market, super marker, etc) competitiveness. In that condition, it’s necessary to get the strategies of competitiveness of the traditional market increased in Bogor Regency. The objective of the research is to formulate strategy and program to achieve more competitiveness of the traditional market in Bogor Regency. The specific aims are to identify the condition of traditional market, to analyze the perception of consumers and to formulate the strategies for the increasing competitiveness of the traditional market in Bogor Regency. Research uses the method that has the descriptive quantitative with it for identificating of condition of the traditional market, IPA Analysis for the Analysis perception customer to traditional market and SWOT and QSPM Analysis for formulating strategy and program to increase competitiveness the traditional market in Bogor Regency. Results of the research show the three priority strategics for increasing the competitiveness of the traditional market in Bogor Regency, those are 1) to increase quality of service market, 2) to increase quality of infrastructure market, and 3) to control the PKL trader those belong to the traditional market.
Keyword: 

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.