Judul: Stabilitas Sisipan Kandidat Gen Toleran Aluminium pada Padi Situ Bagendit Hasil Transformasi secara In-Planta
Abstrak: Situ Bagendit is one of the rice variety that sensitive to aluminum (Al) toxicity. The variety can be developed to an Al-tolerant variety because it can be planted in both dry and wet field. The effort to develop Al tolerant varieties could be done by inserting Al tolerance genes. An Al-tolerance gene, called B11 gene has been inserted to Situ Bagendit genome by in-planta technique and produced T1 generation. The research objective was to evaluate the stability of B11 gene insertion in T1 and T2 generation of rice transgenic SB-16. The evaluation was done by rice seed selection using hygromycin antibiotic with concentration 20 mg/l, molecular analysis using polymerase chain reaction (PCR) technique, and plant phenotype observation. The result of this research showed that B11 gene had been integrated in T1 plant based on molecular analysis and plant phenotype observation. The result of seed selection showed that T2 generation still segregated for B11 gene.
Keyword: Transgenic Rice., Situ Bagendit, B11 Gene, Aluminum Tolerance

Judul: Seleksi Tanaman Padi Transgenik Generasi T1 dan T2 yang Membawa Kandidat Gen Toleransi Aluminium
Abstrak: The use of aluminum (Al) tolerant rice variety is one of the alternative solutions to solve the food crop production problem in acid soils. The Al tolerant rice variety can be developed by genetic engineering. The T1 and T2 generation of transgenic rice has been developed from the transformation of rice using B11 gene, which was an Al tolerance gene candidate. However the true transgenic rice carrying the B11 gene has not been confirmed yet. This research was conducted to select and verify the T1 and T2 transgenic rice which contained the Al tolerant gene candidate. The result showed that the selection media containing paromomycin antibiotic could select the putatif transgenic rice without albinism effect that appeared in selection using canamycin antibiotic. The selection obtained true transgenic lines. The PCR analysis using the primers developed from nptII gene showed a 600 bp DNA band in the transgenic plant, but it was none in the wild type. The plant height and number of tiller characters were not significantly different between the wild type and the transgenic plant.
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Judul: Penggunaan Teorema Homeomorphy 2-Manifold dan Teorema Euler Poincare pada Torus 𝑇�� dan Simplicial Complex 𝐾��
Abstrak: Two dimensional topological spaces are said to be homeomorphic if they have the same topological invariant, where one of topological invariant used is an Euler characteristic. Homeomorphy 2-Manifold’s Theorem and Euler Poincare’s Theorem are used to distinguish two topological spaces. Homeomorphy 2-Manifold’s Theorem uses Euler characteristic to identify two dimensional topological spaces. Euler Poincare’s Theorem is an alternative way to find Euler characteristic with Betti number, which is topological invariant as well. The objective of this paper is to investigate the homeomorphism of torus 𝑇����� and simplicial complex 𝐾�����. Topological space torus and simplicial complex 𝐾����� have the same Euler characteristic, which is two. Based on Homeomorphy 2-Manifold’s Theorem topological space torus 𝑇����� and simplicial complex 𝐾����� is homeomorphic.
Keyword: simplicial, torus, Euler, homeomorphism, topology