Judul: Analysis of Characteristic Working from Home, Subjective Well-Being and Outcomes Working from Home at PT. Asuransi Rama Satria Wibawa Jakarta
Abstrak: Penerapan kebijakan WFH bukanlah suatu metode bekerja di Indonesia, melainkan upaya mitigasi penyebaran COVID-19. PT. Asuransi Rama Satria Wibawa merupakan salah satu perusahaan yang menerapkan kebijakan WFH dan karyawan dituntut untuk tetap menjaga kinerjanya. Selain itu, subjective well-being bagi karyawan yang menjalankan WFH juga perlu diperhatikan dalam meningkatkan outcomes WFH. Penelitian ini bertujuan untuk mengindentifikasi karakteristik responden, menganalisis persepsi characteristic WFH, subjective well-being dan outcomes WFH serta pengaruh characteristic WFH dan subjective well-being terhadap outcomes WFH. Metode penarikan sampel menggunakan sensus dengan jumlah responden sebanyak 46 orang. Penelitian ini menggunakan metode analisis deskriptif dan SEM-PLS. Karyawan pada PT. Asuransi Rama Satria Wibawa didominasi oleh karyawan laki-laki sebanyak 33 orang, rentang usia 31-40 tahun sebanyak 16 orang, berstatus menikah sebanyak 32 orang, lulusan Strata Satu (S1) sebanyak 23 orang dan masa kerja 2-5 tahun sebanyak 30 orang. Persepsi karyawan terhadap characteristic WFH, subjective well-being dan outcomes WFH mayoraitas baik. Hasil analisis SEM-PLS menunjukkan bahwa characteristic WFH dan subjective well-being berpengaruh positif terhadap outcomes WFH., The implementation of WFH policies is not an organizational culture in Indonesia but rather an effort to mitigate the spread of COVID-19. PT. Asuransi Rama Satria Wibawa is one company that implements WFH policies, and employees are required to maintain their performance. Besides, subjective well-being for employees who practice WFH also needs to be considered in improving WFH outcomes. This study aims to identify the characteristic of respondents, analyze the perceptions of WFH characteristics, subjective well-being, and WFH outcomes and the influence of WFH characteristics and subjective well-being on WFH outcomes. The sampling method uses cencus with 46 total respondents. This research uses a descriptive analysis method and SEM-PLS. Employees at PT. Rama Satria Wibawa Insurance is dominated by 33 male employees, 16 people from 31-40 years old, 32 people were married, 23 people graduated from S1 and 30 people working periods of 2-5 years. Employees' perceptions of WFH characteristics, subjective well-being, and WFH outcomes are generally good. The SEM-PLS analysis results show that WFH characteristics and subjective well-being have a positive effect on WFH outcomes.
Keyword: characteristic WFH, outcomes WFH, SEM-PLS, subjective well-being

Judul: Efektivitas Sistem Kerja di Rumah dalam Mendukung Work-Life Balance, Employee Engagement dan Kinerja Karyawan (Studi Kasus di PT Asuransi Adira Dinamika)
Abstrak: Sistem berkerja dari rumah (work from home/WFH) pada suatu perusahaan telah mulai banyak diterapkan pada beberapa dekade terakhir ini. Kecenderungan tersebut mengalami peningkatan pada masa panderni Covid-19. Disamping pertimbangan kesehatan dan keselamatan, WFH ternyata membawa beberapa manfaat dan keuntungan. Dengan kemajuan teknologi informasi. berbagai pekerjaan dapat dikerjakan di mana saja, dengan tingkat penyelesaian yang relatif singkat dan dengan hasil yang baik. Konsep WFH diyakini dapat meningkatkan keseimbangan kerja dan kehidupan (work-life balance) yang berimplikasi kepada kualitas hasil pekerjaan dan kehidupan keluarga yang lebih baik. Namun demikian, di sisi lain WFH dapat berisiko untuk menjadi penyebab berbagai ketidakefektifan kerja karena secara simultan WFH dan work-life balance dapat berpengaruh pada employee engagement yang implikasinya dapat memengaruhi kinerja karyawan. Salah satu perusahaan yang membuat program telework atau bekerja dari rumah/ WFH, adalah Asuransi Adira Dinamika, anak perusahaan Bank Danamon. Karyawan Asuransi Adira Dinamika, hanya diwajibkan bekerja di kantor satu hari dalam sepekan. Sebagian karyawan bekerja separuh waktu di kantor. Rasio pembagian waktu bekerja di kantor bervariasi antara 30-40%. dst..
Keyword: Employee Engagement, Performance, Work from home, Work-Life Balance

Judul: Gene Editing  of Two Cyanide Synthesis-related Genes in Cassava using CRISPR/Cas9
Abstrak: Cassava (Manihot esculenta) contain toxic quantities of cyanogenic glucoside. Reducing the potentially toxic cyanogenic glucoside levels in cassava could be achieved by gene editing through Clustered Regularly Interspaced Short Palindromic Repeats – CRISPR associated protein 9 (CRISPR/Cas9), a high- precision genome editing technique in plants. Two cassava UDP- glucosyltransferase (UGT) paralogs, UGT85K4 and UGT85K5, are the genes catalyzing the synthesis of cyanogenic glucosides in plants. The study aimed to construct the CRISPR/Cas9 recombinant vector carrying single guide RNA (sgRNA) to edit UGT85K4 and UGT85K5 genes, and transform sgRNA CRISPR/Cas9 construct into KU50 cassava calli. Five sgRNAs were designed, considering the on/off target efficiency score and GC content. Those sgRNAs were ligated into pRGEB32 plasmid and introduced to competent Escherichia coli DH5a. The PCR colony and DNA sequencing were performed to verify the sgRNA insert. The amplicons showed the expected band size of recombinant vector (180 bp). Based on sequencing results, only one sgRNA was successfully ligated into the plasmid that will be used to edit the UGT85K5 gene. The sgRNA CRISPR/Cas9 construct in pRGEB32 plasmid was successfully transformed into Agrobacterium tumefaciens and introduced into KU50 cassava calli through co-cultivation. The overgrowth of Agrobacterium occurred after the co-cultivation phase.
Keyword: CRISPR/Cas9, Cyanogenic glucoside, Gene editing, sgRNA