Judul: In silico Analysis of Active Compounds in Ketepeng Cina Plant (Cassia alata) as an Anti-Candida Candidate
Abstrak: Penelitian ini mengevaluasi potensi senyawa aktif dari tanaman ketepeng cina (Cassia alata) sebagai kandidat obat anti-candida menggunakan pendekatan in silico. Studi ini bertujuan mengidentifikasi dan menganalisis interaksi senyawa aktif asal ekstrak tanaman ketepeng cina dan menginteraksikannya dengan enzim lanosterol 14a-demetilase, yang merupakan target utama dalam terapi infeksi candidiasis. Metode yang digunakan meliputi penambatan molekuler (molecular docking) untuk menentukan afinitas dan stabilitas interaksi ligan dengan reseptor. Hasil menunjukkan bahwa senyawa stigmasterol berpotensi sebagai inhibitor enzim lanosterol 14a-demetilase. Stigmasterol mampu berinteraksi dengan sisi aktif Tyr126 pada reseptor dengan nilai energi afinitas yang lebih negatif dibandingkan dengan gentian violet sebagai ligan pembanding., This study evaluates the potential of active compounds from the ketepeng cina (Cassia alata) plant as an anti-candida drug candidates using an in silico approach. The study aims to identify and analyze the interactions of active compounds from ketepeng cina extract and the enzyme lanosterol 14a-demethylase, a primary target in candidiasis therapy. The methods used include molecular docking to determine the affinity and stability of ligand-receptor interactions. The results indicate that stigmasterol compound has potential as inhibitor of the lanosterol 14a-demethylase enzyme. Stigmasterol can interact with the active site Tyr126 on the receptor with more negative affinity energy values compared to gentian violet as the reference ligand.
Keyword: Cassia alata, In silico, anti-candida, ketepeng cina, lanosterol 14a-demetilase, lanosterol 14a-demethylase

Judul: Potensi Senyawa Aktif Sungkai (Peroema canescens) dalam Menginhibisi Enzim Beta-Laktamase Secara In Silico.
Abstrak: Kasus resistensi bakteri terhadap antibiotik menjadi ancaman serius bagi kesehatan manusia karena menurunkan efektivitas obat untuk mencegah dan menyembuhkan infeksi bakteri. Resistensi dapat disebabkan oleh pengobatan yang tidak tuntas. Salah satunya yaitu bakteri Escherichia coli yang menghasilkan enzim CTX-M yang merupakan jenis dari beta-laktamase yang menghambat kerja antibiotik golongan beta-laktam. Sungkai merupakan salah satu tanaman obat tradisional dan terbukti menghambat pertumbuhan bakteri Escherichia coli. Penelitian ini bertujuan mengidentifikasi senyawa aktif sungkai yang berpotensi menginhibisi beta-laktamase serta analisis interaksi yang terjadi secara in silico. Senyawa aktif daun sungkai ditambatkan ke reseptor beta-laktamase, kemudian dianalisis interaksinya serta diprediksi bioavailabilitas dan toksisitas. Hasil penelitian menunjukan sebanyak 6 dari 19 ligan uji lolos penapisan hingga akhir dan berpotensi menjadi inhibitor beta-laktamase. Senyawa tersebut antara lain, beta-sitosterol, 3,4’,5-thrihydroxy-3,’,7-dimenthoxyflavanone, physcion, isorhamnetin, chrysoeriol, quercetin 3,3 dimetly ether, dan vintexin-2-O rhamnoside., Cases of bacterial resistance to antibiotic has become a serious threat for human health because they reduce the effectiveness of drugs in preventing and treating bacterial infections. Resistance can be caused by uncomplete treatment. One of example is Escherichia coli bacteria which produces the CTX-M enzyme, that is a type of of beta-lactamase that inhibits the action of beta-lactam antibiotics. Sungkai is one of the traditional medicinal PLANTS and it is proved inhibiting the growth of Escherichia coli. This research aims to identify the active compounds of sungkai that have the potential to inhibits beta-lactamase and analyze interactions of molecular docking. The active compounds of sungkai leaves are docked to the beta-lactamase receptor, then their interactions are analyzed, predicting bioavailability, and toxicity. The results showed that 6 of the 19 tested ligands pass the screening and have the potential to become beta-lactamase inhibitors. The compounds are beta-sitosterol, 3,4’,5-thrihydroxy-3’7-dimenthoxyflavanone, physcion, isorhmaetin, chrysoeriol, quercetin 3,3 dimetly ether, and vintexin-2-O-rhamnoside.
Keyword: resistent, beta-lactamase inhibitors, sungkai

Judul: Approximation of the Median of Poisson Distribution Using the Relation of Poisson-Gamma Distribution
Abstrak: A major drawback of median is that median cannot usually be defined in closed form, even in the cases where their distribution functions are known. This study gives an approximation of the closed form for the median of the Poisson distribution by using the relation of Poisson-Gamma distribution. The approximation uses elementary techniques based on the monotonicity of certain sequences involving tail probabilities of the Poisson distribution and the Central Limit Theorem. Furthermore, a closed form expression for the mean absolute deviation 􀜧|􀜰􀰒 􀵆 􀜯􀝁􀝀􁈺􀜰􀰒􁈻| is also studied. The median is relevant because of the fact that it minimizes the mean absolute deviation of a Poisson random variable (􀜰􀰒􁈻 by considering median as central tendency.
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