Judul: Uji kinerja spektrofotometer ultraviolet-tampak berkas ganda terhadap pengukuran ambroksol HCI pada tablet Eskpektoran
Abstrak: Performance test of double beam ultraviolet-visible (UV-Vis)Spectrophotometer on ambroxol HCI, measurement in expectorant table inculded two steps. The first test included wavelength accuracy, stray light, resolution, noise, baseline flatness,
Keyword: 

Judul: Development of Portable Spectrophotometers and Performance Evaluation of Ponceau 4R Food Colorants Substances
Abstrak: Spektrofotometer dapat dikembangkan dari komponen elektronik yang tersedia secara umum menjadi spektrofotometer portabel. Penelitian ini bertujuan mengembangkan spektrofotometer jinjing dan menentukan uji kinerja analitiknya. Spetrofotometer jinjing yang disusun oleh komponen utama yang terdiri dari lampu light emitting diode sebagai sumber cahaya, lapisan optik digital versatile disks sebagai kisi, web camera Logitech c920 sebagai detektor dan akuisisi data melalui perangkat lunak Thereminospectro. Uji kinerja spektro menggunakan larutan standar Ponceau 4R dan dibandingkan dengan spektrofotometer komersial Hitachi Spectro U-2800. Spektrofotometer jinjing dengan ukuran optimum menghasilkan spektrum larutan standar yang sebanding dari hitachi spectro u-2800. Keduanya menunjukkan linearitas yang sangat baik karena memenuhi syarat keberterimaan linearitas pada metode spektrofotometri. Dengan demikian, spektrofotometer jinjing yang dikembangkan dapat menjadi alternatif untuk spektrofotometer komersial. Kata kunci: digital versatile disks, light emitting diode, ponceau 4R, spektrofotometer, web camera, Spectrophotometers can be expanded from commonly available electronic components to portable spectrophotometers. This study aims to develop a portable spectrophotometer and determine its analytical performance test. A portable spectrophotometer composed of main components consisting of a light-emitting diode lamp as a light source, an optical layer of digital versatile disks as a grid, a Logitech c920 web camera as a detector, and data acquisition via the Thereminospectro software. Spectro performance test using standard solution Ponceau 4R and compared with a commercial spectrophotometer Hitachi Spectro U-2800. The portable spectrophotometer with the optimum size produces a standard solution spectrum comparable to that of the Hitachi Spectro u-2800. Both of them show very good linearity because they meet the requirements for linearity acceptance in the spectrophotometric method. Thus, the developed portable spectrophotometer can be an alternative to commercial spectrophotometers. Keywords: digital versatile disks, light emitting diode, ponceau 4R, spectrophotometer, web camera
Keyword: Spektrofotometer UV-VIS, Spektrofotometer jinjing, LED, DVD, Webcamera, Ponceau 4R

Judul: Mitochondrial DNA Gene Organization and Phylogenetic Relationship of Naja siamensis
Abstrak: Naja siamensis is one of Asiatic cobra in the Family of Elapidae, known as a cryptic snake. It has many morphological variations that lead to misidentification. Misidentification can cause the wrong concept in developing antivenom types due to high snakebite cases occur in Thailand. Thus, an accurate species identification using a molecular approach is crucially required. Mitochondrial DNA has been used widely in snake molecular studies. These studies were aimed to determine the arrangement of the N. siamensis partial mitochondrial genes using reptile-oriented primers and to reveal the phylogenetic relationship among other species of Asian cobra. DNA extraction was carried out based on the standard DNA salting-out protocol and was conducted by bioinformatics analysis using BLASTn and MEGA 6 software. Gene annotation was performed against N. kaouthia complete genome. A total of 3121 bp of N. siamensis mtDNA genes were sequenced consisting of partial of six regions: 16S rRNA, ND1, tRNA-Ile, CRII region, tRNA-Leu, tRNA-Gln, and COI genes. The mtDNA gene organization of N. siamensis was predicted to has a similar pattern with the common Elapidae mtDNA gene organization. This study showed that there is a monophyletic form in Naja genus and N. kaouthia is the closest species with N. siamensis based on partial 16S rRNA and ND1 gene.
Keyword: 16S rRNA, Elapidae, ND1, phylogenetic relationship