Judul: Stabilitas dan efektivitas biosensor glukosa berbasis Escherichia coli menggunakan imobilisasi glutaraldehida
Abstrak: The immobilization glucose dehydrogenase (GDH) cells from Escherichia coli cross-linking glutaraldehyde on a carbon paste electrode were studied. Cyclic voltammetry are employed to investigate the catalytic behavior of the biosensor. The analysis used for the optimization of GDH activity was response surface method. Optimum conditions for GDH activity was at temperature 30 oC, pH 6, glucose 10.25 mM, and pyrroloquinoline quinone (PQQ) 3.05μM for E. coli cells without cross-link with glutaraldehyde. On the other hand, the optimum conditions for GDH activity of E. coli cells cross-linked with glutaraldehyde was at temperature 30 oC, pH 6, glucose 10.25 mM, PQQ 3.05 μM, and glutaraldehyde 12.5 μL. Dehydrogenase reaction kinetics of GDH catalyzed by glucose followed the Lineweaver-Burk kinetics. KM app value of bacterial E. coli cells cross-linked with glutaraldehyde at 1.1162 mM was smaller than without cross-link, which was 1.9007 mM. Stability of activity GDH E. coli cells cross-linked with glutaraldehyde was 68% higher than that without cross-link.
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Judul: Stabilitas dan Efektivitas Biosensor Glukosa Menggunakan Escherichia coli Yang Diimobilisasi Pada Matriks Nanokomposit Zeolit
Abstrak: Biosensor combines the selectivity of biological recognition element and the sensitivity of the transducer. The application of biosensor as blood glucose measurer for diabetes mellitus patients uses purified enzyme as biological sensor. The substitution of purified enzyme with the microbial cells producing enzymes as biological sensor is carried out to overcome the weaknesses of purified enzyme such as the low stability of the enzyme activity and the high cost. This research was carried out to measure the activity of glucose dehydrogenase (GDH)–the enzyme reacting specifically with glucose as substrate and binding with both pyrolloquinoline quinone (PQQ) and Mg2+ ion as prosthetic group and activator respectively-which is exhibited by escherichia coli, using electrochemical methods Design of experiments only to optimalization the activity of GDH bacterial cells of e. coli is a method of surface response. Optimum conditions of activity of GDH derived cells of e. coli bacteria in the absence of a matrix of nanocomposite zeolite on the process of immobilization is the temperature of 30°C, pH 6, concentration of glucose 15 mM, the concentration of PQQ 3,050 μM. As for the optimum conditions for the activity of GDH bacterial cells of e. coli with matrix nanocomposite zeolite is the temperature of 30°C, pH 6, glucose concentration of 15 mM, the concentration of PQQ 3,050 μM, and nanocomposite zeolite 137,50 mg. The reaction catalyzed by GDH kinetics of Glucose Kinetics equation follows the Lineweaver-Burk. The value of KM app bacterial cells of e. coli with the larger of nanocomposite zeolite mM compared to 1,4580 without nanocomposite of zeolite matrix 0,720 mM. This shows that affinity GDH e. coli immobilization of glucose substrate without nanocomposite zeolite is greater compared to the matrix nanocomposite zeolite.GDH activity stability of bacterial cells is not much different compared with no matrix nanocomposite zeolite.
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Judul: Analisis Kelayakan Investasi Pembelian Pesawat N-219 oleh PT Pelita Air Service untuk Penerbangan Perintis di Manokwari, Papua Barat.
Abstrak: PT Pelita Air Service (PT PAS) sebagai anak perusahaan PT Pertamina (persero) yang bergerak di bidang industri aviasi memiliki empat pilar bisnis yang dijalani. Salah satu bisnis utama PT PAS adalah bisnis air charter yang nantinya dikembangkan menjadi bisnis penerbangan perintis. Dengan melihat laju pertumbuhan penumpang yang baik dan untuk meningkatkan konektivitas antar daerah di Indonesia, bisnis penerbangan perintis akan besar potensinya untuk PT PAS mengembangkan bisnisnya. Tujuan dari penelitian ini adalah untuk mengetahui kelayakan bisnis perintis yang akan dilakukan. Penelitian ini dilakukan dengan metode analisis aspek non finansial dan finansial. Hasil menunjukkan untuk aspek non finansial untuk aspek teknis dinilai tidak layak untuk melakukan bisnis. Sedangkan untuk aspek pemasaran, manajemen, hukum, dan aspek finansial dinilai layak. Untuk aspek finansial didapat nilai NPV adalah Rp23 879 575 648, IRR sebesar 20%, Net B/C 1.942, dan PP selama 9.2 tahun.
Keyword: Analisis Kelayakan, Aspek Finansial, Aspek Non Finansial