Judul: Pembuatan Tepung Tapioka dengan Pengering Semprot dan Pengering Kabinet serta Aplikasinya pada Produk Pilus di PT Garudafood Putra Putri Jaya
Abstrak: Tapioca produced by means of modern method is not suitable for making of pilus. Pilus which is produced by using the so called “modern tapioca” will have a hard texture and not fluffy. Traditional tapioca flour is made with a long time to get a distinctive taste. However, traditional process of tapioca production could not be applied in large production scale. Therefore, experiments were conducted to produce tapioca starch of industrial scale by means of spray drying and cabinet drying process. During drying with spray dryer, three inlet temperature levels in range 140-160 °C, 170-190 °C, 200-220 °C were applied, whereas during drying with cabinet dryer, three levels of temperature in range 40-60 °C, 60-70 °C, 70-90 °C and three levels of drying time 0.75 hour, 1.5 hours, 2.5 hours were applied. The starch properties analysed were tapioca starch yield, moisture content, whiteness, fineness and gelatinization pattern of starch. The tapioca starch obtained will be applied in manufacturing of “pilus”. The characteristics of “pilus” evaluated is crispiness.
Keyword: pilus., cabinet drying, spray drying, tapioca

Judul: Karakteristik Kerenyahan Pilus dari Dua Tepung Tapioka
Abstrak: Quality of a product is one of the most important aspects to determine consumer’s acceptance. Final product quality closely related to the quality of raw materials used. Garudafood as one of the leading food and beverage companies in Indonesia, has pilus as its main snack product. Pilus is a fried round-shaped snack made from tapioca with a mixture of other spices, ready to be consumed as a snack or side dish companion. Pilus is supposed to has a crispy texture. Garudafood needs 2000 tons of tapioca for pilus production per month. However, this necessity could not be filled with a consistent quality of the material supply. Thus, characterise the tapioca used in production to determine the test parameters that can predict pilus crispness need to be done. The parameters tested are analysis of starch level, amylose, amylopectin, swelling power, starch gelatinization profiles, texture analyzer, and organoleptic test. Moreover, the test be repeated at each arrival tapioca to see the consistency of raw materials quality. The research showed that most relevant tests to specify pilus crispness are the ratio of amylose amylopectin and peak viscosity on starch gelatinization profiles. In addition, the data indicates that the quality of raw materials used are not consistent.
Keyword: tapioca, starch, pilus, amylose, Bogor Agricultural University (IPB)

Judul: Mitochondrial DNA Gene Organization and Phylogenetic Relationship of Naja siamensis
Abstrak: Naja siamensis is one of Asiatic cobra in the Family of Elapidae, known as a cryptic snake. It has many morphological variations that lead to misidentification. Misidentification can cause the wrong concept in developing antivenom types due to high snakebite cases occur in Thailand. Thus, an accurate species identification using a molecular approach is crucially required. Mitochondrial DNA has been used widely in snake molecular studies. These studies were aimed to determine the arrangement of the N. siamensis partial mitochondrial genes using reptile-oriented primers and to reveal the phylogenetic relationship among other species of Asian cobra. DNA extraction was carried out based on the standard DNA salting-out protocol and was conducted by bioinformatics analysis using BLASTn and MEGA 6 software. Gene annotation was performed against N. kaouthia complete genome. A total of 3121 bp of N. siamensis mtDNA genes were sequenced consisting of partial of six regions: 16S rRNA, ND1, tRNA-Ile, CRII region, tRNA-Leu, tRNA-Gln, and COI genes. The mtDNA gene organization of N. siamensis was predicted to has a similar pattern with the common Elapidae mtDNA gene organization. This study showed that there is a monophyletic form in Naja genus and N. kaouthia is the closest species with N. siamensis based on partial 16S rRNA and ND1 gene.
Keyword: 16S rRNA, Elapidae, ND1, phylogenetic relationship