Judul: Ketahanan Bakteri Probiotik Bifidobacterium Longum Bf-1 Yang Dienkapsulasi Nano Alginat Terhadap Cairan Lambung Dan Usus Halus Simulasi.
Abstrak: Nanoenkapsulasi merupakan salah satu metode dalam mempertahankan viabilitas probiotik di kondisi saluran pencernaan manusia. Tujuan dari penelitian ini adalah untuk mempertahankan populasi bakteri probiotik Bifidobacterium longum BF-1 berlapis kitosan dan nano alginat dalam cairan lambung dan usus halus simulasi. Penggunaan kitosan 2 mg/mL pH 6 menghasilkan probiotik dengan populasi tertinggi (14.27 log cfu/ml), zeta potensial positif, dan ukuran partikel terkecil (1351 nm) dibandingkan dengan perlakuan lainnya (P<0.05). Pembuatan nano alginat dengan RAL 3x ulangan menggunakan konsentrasi larutan alginat 1 mg/mL dan CaCl2 dengan berbagai konsentrasi (0.5-1.5 mg/ml). CaCl2 dengan konsentrasi 0.5 mg/mL menghasilkan nanopartikel alginat terkecil (339.4 nm) dibandingkan perlakuan lainnya (P<0.05). Proses enkapsulasi dilakukan dengan mengoptimasi probiotik berlapis kitosan dan nano alginat terpilih, lalu diuji ketahanannya dalam cairan lambung dan usus halus simulasi. Proses enkapsulasi dengan nano alginat dan coating kitosan dapat mempertahankan viabilitas probiotik yang lebih baik dibandingkan dengan sel probiotik bebas tanpa enkapsulasi (P<0.05) dengan populasi akhir >7 log cfu/ml.
Keyword: nanoenkapsulasi, Bifidobacterium longum, alginat, kitosan

Judul: Mikroenkapsulasi Lactobacillus plantarum MB427 dalam alginat-kitosan meningkatkan sintasan dan umur simpan selai salak probiotik
Abstrak: Selai salak dapat digunakan sebagai makanan pembawa probiotik (Lactobacillus plantarum MB427). Tujuan penelitian adalah meningkatkan sintasan dan umur simpan selai salak probiotik dengan mengenkapsulasi L. plantarum MB427 dalam alginat 3% dilanjutkan dengan penyalutan menggunakan kitosan. Konsentrasi kitosan terbaik ditentukan menggunakan berbagai konsentrasi (0.4, 0.6, 0.8, 1.0, 1.2, 1.5, dan 2.0%) setelah dipapar pada suhu 50oC, 20 menit. Pemaparan menghasilkan sintasan probiotik pada konsentrasi 0.4, 0.6, 0.8% menurun sebesar 1 log, sedangkan konsentrasi 1.0, 1.2, 1.5, 2.0% tidak mengalami penurunan nyata (p>0.05). Tahap kedua adalah menentukan umur simpan selai salak probiotik pada suhu 15oC yang dikemas dalam plastik HDPE steril. Sebanyak 10% b/b probiotik bebas/mikrokapsul alginat/ alginat-kitosan dimasukkan dalam selai salak. Teknik mikroenkapsulasi dapat meningkatkan sintasan probiotik. Viabilitas probiotik bebas sebesar 6.73 log cfu/g hanya bertahan sampai minggu ke 3. Pada minggu ke-5 viabilitas probiotik alginat sebesar 6.71 log cfu/g, sedangkan viabilitas probiotik alginat-kitosan sebesar 6.29 log cfu/g dapat dipertahankan sampai mingggu ke 6. Interpolasi waktu penyimpanan selai salak saat viabilitas probiotik mencapai 6 log cfu/g untuk probiotik bebas, mikrokapsul alginat, dan alginat-kitosan masing-masing adalah 27, 42, dan 47 hari. Total kapang khamir hingga minggu ke 6 sebesar 1.90 log cfu/g. Hasil uji statistik untuk pH, aw, kadar air, dan total padatan terlarut (TPT) tidak signifikan terhadap kontrol (selai tanpa probiotik). Selama penyimpanan terjadi penurunan pH serta peningkatan TPT, aw, dan kadar air. Selama penyimpanan juga terjadi perubahan nyata pada evaluasi sensori selai probiotik bebas (rasa) dan selai mikrokapsul alginat-kitosan (tekstur). Nilai kapasitas antioksidan selai salak (51.76%) berbeda nyata lebih rendah 27% dari buah salak segar (70.82%). Kata kunci: mikroenkapsulasi, kitosan, Lactobacillus plantarum MB427
Keyword: Microencapsulation, Lactobacillus plantarum mb427, Konsentrasi kitosan probiotic, Mikro kapsul probiotik

Judul: Characteristic of Overshooting Top (OT) Based on  Himawari  8  Satellite  as  a  Convective  Cloud  Feature  that  Generates  Extreme Weather in the Java Island Region
Abstrak: Overshooting Top (OT) is a feature at the top of a convective cloud that is associated with extreme weather. Research on OT in tropical Indonesia is still very limited. The existence of geostationary satellites with wide area coverage and fast frequency observations is useful for observing the fast dynamics of the OT life cycle. Therefore, this study intends to analyze the relationship between OT and extreme weather, updraft and thermodynamic conditions of OT when extreme weather occurs, the best objective method for detecting OT in the day time and night time, and the spatiotemporal distribution of OT in the tropical region of Java Island during the 2018-2021 period. Based on visual analysis using visible channel and Himawari 8 sandwich products, 87% of extreme weather events in Java were accompanied by the appearance of OT. It indicates that the presence of the OT pattern is a convection cloud pattern or feature that is closely related to extreme weather events for the Java Island region. The existence of a time lag between the appearance of OT and extreme weather events on Java Island indicates that the OT of convection cloud can be used as a precursor or nowcasting for the appearance of extreme weather. Most of the convective cloud OT features during extreme weather events on Java Island have altitude that were only able to exceed the altitude of the equilibrium level and few were able to exceed the tropopause level of 9 km – 16 km. The difference in atmospheric conditions during extreme weather events on Java Island, which is in the tropics and subtropics, is a strong and unstable convective energy condition characterized by a larger CAPE, as well as unstable KI, LI, SI and TTI values. It is offset by kinematic factors in the form of a weak vertical wind shear (bulk wind shear 0-6 km) and a higher freezing point height. The difference between extreme weather events in the Java Island region and other tropical regions compared to subtropical regions is the strong updraft speed of convection clouds. The IRW Texture COMB algorithm based on the IRW-Texture Gradient modification and the COMB algorithm shows a significant increase in quality for detecting OT and non-OT pixels in Java. Rainy days with rainfall > 50 mm/day are very common in areas with an altitude of > 500 m. On the other hand, the level of convection activity which is indicated by the presence of OT and lightning is significantly reduced in this area. OT of convective clouds appear more frequently during the DJF season and less frequently during the JJA season. OT often appears during the day time and rarely at night time. Most OTs appear in the central part, away from the south and north coasts of Java Island during the day, while OTs that appear near the south and north coasts of Java Island can appear in the morning or afternoon.
Keyword: convective clouds, extreme weather, Overshooting Tops, Himawari 8 Satellite