Judul: Study About Factors Influencing Transition of Green Open Spaces Based on Analysis of Land Use in Depok City, West Java, Indonesia
Abstrak: This study focused on land development in Depok City located south of Jakarta. Although Depok enjoys a tropical climate, it is necessary to consider the distribution of land use within the city, especially green open spaces. Depok is a commercial center and a public utilities center, where water is stored to supply ground water as buffer city of Jakarta. Moreover, it is a hotspot of education and residential area development. Depok is influenced strongly by land development in Jakarta, and further economic growth is expected. The purpose of this study is to understand the transition of green open spaces and land use types in Depok to reveal the changes in the of area of green open spaces in Depok and to identify the factors influencing the transition of green open spaces in Depok. To identify the factors influencing the transition of green open spaces, binomial logistic regression analysis was used. The dependent variable was the area of green open space converted into non-green open space, and the independent variables included distance from commercial facilities, distance from industrial areas, distance from educational areas, distance from hospitals, distance from rivers, distance from lakes, distance from main roads, distance from a highway, distance from a highway entrance, distance from a railroad station, and distance from inundation-prone areas. It was revealed that the area of green open space has rapidly decreased in Depok, especially from 2005 to 2015. Two factors particularly influenced the transition of green open spaces, where a greater proximity to educational areas or main roads was associated with a greater reduction in green open space. Also, the distance from hospitals and lakes were associated with the maintenance of green open spaces in Depok. For residents, convenience is improved in areas close to educational areas or main roads; therefore, it is conceivable that buildings such as housings are increasing accordingly in such areas. Keywords:binomial logistic regression analysis, green open space, land use
Keyword: Binomial logistic regression analysis, Green open space, Land use

Judul: Analisis Perubahan Penggunaan Lahan dan Perkembangan Wilayah pada Lingkungan Kota Depok
Abstrak: Kota Depok mengalami perkembangan wilayah dan urbanisasi yang dipengaruhi perluasan perkotaan Jakarta. Hal ini terlihat dari adanya perubahan penggunaan lahan pertanian ke non pertanian yang berdampak pada kualitas lingkungan. Penelitian ini bertujuan mengidentifikasi perubahan penggunaan lahan tahun 2011-2018, menganalisis kualitas lingkungan dengan Local Environmental Sustainability Index (LESI) dan menganalisis faktor-faktor yang mempengaruhi kualitas lingkungan di Kota Depok. Analisis perubahan penggunaan lahan dilakukan dengan overlay (SIG) peta penggunaan lahan tahun 2011 dan 2018 serta menggunakan tabulasi matriks perubahan lahan. Penetapan Indeks Kualitas lingkungan (LESI) didasarkan metode yang dikembangkan Pravitasari et al. (2016). Faktor-faktor yang mempengaruhi kualitas lingkungan wilayah dianalisis dengan regresi berganda dimana salah satu variabelnya adalah indeks perkembangan wilayah (IPW) tiap kelurahan dari hasil skalogram. Ruang terbuka hijau, permukiman dan jasa/industri mengalami peningkatan luas dari tahun 2011 ke 2018. Luas lahan sawah dan pertanian lahan kering mengalami penurunan. Pengelolaan urbanisasi yang disertai bertambahnya lahan terbangun dan ruang terbuka hijau (RTH) di Depok telah semakin berpengaruh signifikan meningkatkan kualitas lingkungan di Kota Depok., Urbanization in Jakarta and its surroundings has influenced the development of Depok City. An indication of the ongoing urbanization process is the change in land use from agriculture to non-agriculture. These conditions can affect the land quality and environment. This study aims to identify land use changes in 2011-2018, to analyze environmental quality with the Local Environmental Sustainability Index (LESI) and to analyze factors that affect environmental quality in Depok City. Land use change analysis was carried out by overlaying (GIS) land use maps from 2011 to 2018 and using a land change matrix tabulation. The factors that affect the quality of the regional environment were analyzed by multiple regression in which one of the independent variables was the regional development index (IPW) of each urban village (kelurahan) from the scalogram results. Green open space, settlements, and services/industries have increased in area from 2011 to 2018. On the other hand, the area of rice fields and dry land agriculture has decreased. The increase in built-up land in Depok City shows that the urbanization process is being/has occurred. Depok City. The management of green open space (RTH) has significantly improved the environment quality.
Keyword: Urbanisasi, Lahan terbangun, Ruang Terbuka Hijau, LESI

Judul: Isolasi dan purifikasi inhibitor RNA helikase virus hepatitis C dari bakteriosin bakteri asam laktat S34
Abstrak: Target molecular therapy is one of method to prevent hepatitis C virus which was developed recently. NS3 Helicase/RNA helicase hepatitis C virus inhibition using inhibitory peptide are target in molecular therapy. Bacteriocin are inhibitory peptides which can inhibit hepatitis C virus through inhibition of NS3 helicase. Bacteriocin is a peptide which is produced by lactic acid bacteria. The aim of this research were to isolate and to purify bacteriocin from S34 lactic acid bacteria which has high activity to inhibit RNA helicase of hepatitis C virus. S34 lactic acid bacteria were identified using 16S RNA amplification and DNA sequencing identification of S34 lactic acid bacteria was Lactobacillus plantarum WCFS1. The bacterial growth reached logaritmic phase at 7 hours and stationary phase at 10 hours. Bacteriocin were isolated and purified by ammonium sulphate precipitation with 80% saturation, gel filtration chromatography, and characterization of bacteriocin through molecular weight analysis and measured protein concentration. Bacteriocin from S34 lactic acid bacteria were included in 2nd class bacteriocin, had 64.20 % of inhibition against RNA helicase hepatitis C virus, had molecular weight about 1.7-4.2 kDa, and specific activity in the amount of 2.84 x 106 U/mg.
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