Judul: Pengaruh Wanita Bekerja terhadap Keberhasilan Pendidikan Formal Anak (Kasus di Perumahan Sawati Mas, Desa Cipondok, Kecamatan Sukaresik, Kabupaten Tasikmalaya, Provinsi Jawa Barat)
Abstrak: The purpose of this research are to analyze the effect of working woman towards child’s formal education successfull. Respondent of research are 32 women who live at Perumahan Sawati Mas, Desa Cipondok, Kecamatan Sukaresik, Kabupaten Tasikmalaya, West Java, who have been married and who have school age children. The research use quantitative and qualitative methods. Reseach also uses Rank-Spearman statistic test to examine correlation between variables. The result of research shows that effect of supporting factors towards woman work time. Woman work time effect towards child’s misbehavior and woman working conditions. Woman working conditions effect towards women’s decision making. Women’s decision making effect toward child’s misbehavior and success of child’s formal education.
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Judul: Faktor-faktor yang Mempengaruhi Strategi Perempuan Bekerja dan Kesejahteraan Keluarga
Abstrak: The main purpose of this research was to know the factors that influence working women’s strategies and family welfare. The respondents were working women, have a position in a compony or institute, and have a family, consist of husband and a child or children. The study design used was a cross sectional study. The sample of this study are 30 working women in Makassar in May until June 2010. The results showed that most of respondents balanced between work and family activities. Results showed that the community and extended families surrounded respondents supported them very well through providing social supports. Working women’s strategy had significant influenced on the total of respondent’s income, respondent’s education, the numbers of children, and numbers of children under five. The family welfare had significant influenced on respondent’s education, the total of respondent’s income, interaction among husband and wife, and gender role in family.
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Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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