Judul: Hubungan Pola Konsumsi Pangan dan Aktivitas Fisik Anak Sekolah dengan Status Gizi Lebih di Daerah Perkotaan dan Perdesaan Bogor
Abstrak: The prevalence of obesity continues to increase, including in childhood, not only in urban areas (10.4%) but also in rural areas (8.1%). The purpose of this research was to analyze the relationship between the pattern of food consumption and physical activity of elementary school children with overweight and obese status in urban and rural areas of Bogor. The research was conducted by applying a cross sectional study design. Subjects consist of 60 elementary school children of 4th and 5th graders in SDN Polisi 5 and SDN Babakan Dramaga 4, that had overweight and obese status, and in good health. Data was collected include child and family characteristics, patterns of consumption and physical activity. The average z-score (BMI/A), energy intake, protein intake and fat intake was higher in urban areas than in rural areas, whereas intake of carbohydrates was lower in urban than rural. The majority of physical activity in urban areas and rural were in the mild category. Family income, age of parents and children allowance of urban area were higher significantly (p<0.05) than rural area. There were a significant correlation between maternal nutritional status (p=0.040, r=0.266), energy intake (p=0.000, r=0.505), protein intake (p=0.003, r=0.381), and fat intake (p=0.001, r=0.426) with overweight and obese status of children.
Keyword: overweight and obese, physical activity, consumption patterns

Judul: Asupan Energi, Zat Gizi dan Serat serta Aktivitas Fisik Siswa Sekolah Dasar Berstatus Gizi Lebih di Kota Bogor
Abstrak: This study were aimed to analyze energy, nutrient intake and physical activity of overweight and obese elementary school student and correlation between energy, nutrient intake, physical activity and family characteristics of students with nutritional status BMI/U. A cross sectional study of 99 subjects was conducted. The intake of energy, protein, fat and carbohydrate was 1777 kcal, 57 g, 53 g and 340 g. Most of the students were deficit of energy, fat, calcium, phosporus, vitamin A, vitamin C and fiber, while protein, carbohydrate and iron were adequate. Physical activity levels of students classified mostly light or sedentary activity. There was no significant relationship between energy and macronutrients intake with BMI/U (P> 0.05). There was a significant correlation between the nutritional status of IMT/U with a family income level, father's education and maternal education (p<0.05).
Keyword: overweight and obesity physical activity, nutrient intake, elementary school student

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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