Judul: Spatial Distribution of Traces Activity and Use of Barking Deer (Muntiacus muntjak) by the Anak Dalam Tribe in Bukit Duabelas National Park
Abstrak: Kijang merupakan salah satu satwa yang dimanfaatkan SAD di Taman Nasional Bukit Duabelas. Namun, data pemanfaatan tersebut masih belum diketahui. Maka dari itu, diperlukan penelitian untuk mengetahui sebaran kijang serta perburuan dan pemanfaatan kijang oleh SAD di Taman Nasional Bukit Duabelas. Metode yang digunakan yaitu pengamatan langsung menggunakan line transect sebanyak 9 jalur sepanjang 1 km dan lebar ±200 m untuk setiap jalur, serta wawancara mendalam terhadap 40 responden. Jenis jejak aktivitas kijang yang ditemukan yaitu tapak, gesekan ranggah, dan bekas makan. Jenis jejak yang ditemukan memiliki sebaran spasial homogen/seragam dan terdapat hubungan yang signifikan dengan keberadaan tumbuhan pakan. Perburuan kijang oleh SAD banyak dilakukan pada malam hari (60%) dengan mengejar/mengintai langsung (60%) menggunakan kecepek (senapan rakitan) (52,5%). Kijang hasil perburuan dominan dimanfaatkan untuk konsumsi sebagai sumber protein hewani dan dijual guna pemenuhan ekonomi (62%). Adapun bagian yang paling banyak dimanfaatkan yaitu bagian daging (65%) yang diolah dengan cara direbus, bakar, goreng, dan salai.
Keyword: Anak Dalam tribe, barking deer, spatial distribution, use

Judul: Spatial Distribution of Traces  Activity and Utilization of Great Wild Boar (Sus scrofa Linnaeus, 1758) by The  Suku Anak Dalam Tribe in Bukit Duabelas National Park
Abstrak: Babi hutan (Sus scrofa) merupakan satwa buruan yang dimanfaatkan masyarakat Suku Anak Dalam di Taman Nasional Bukit Duabelas. Namun data terkait sebaran spasial jejak aktivitas, peburuan serta pemanfaatan babi hutan masih terbatas. Oleh karena itu, penelitian ini bertujuan untuk mengetahui bentuk sebaran spasial jejak aktivitas babi hutan serta mengidentifikasi bentuk hubungan antara perburuan dan pemanfaatan babi hutan oleh SAD dengan karakteristik responden. Metode yang dilakukan pada penelitian ini adalah pengamatan langsung, analisis vegetasi, dan wawancara mendalam terhadap 40 responden. Hasil penelitian menunjukkan bahwa jenis jejak aktivitas babi hutan yang teramati di TNBD yaitu bekas pakan, gesekan tubuh, asahan taring, jejak tapak kaki, kubangan, dan sarang. Jenis jejak aktivitas yang paling sering ditemukan adalah jejak tapak kaki sebanyak 25 jejak (65,8%). Sebaran spasial jejak aktivitas babi hutan di TNBD memiliki bentuk mengelompok (Ip>0). Perburuan babi hutan oleh SAD dominan dilakukan pada siang hari (45%) menggunakan teknik modern dan tradisional. Bagian yang dimanfaatkan yaitu semua bagian sebesar 77%, hasil buruan babi hutan dominan dimanfaatkan SAD dalam bentuk konsumsi dan dagang., Wild boar (Sus scrofa) is a game animal utilized by the SAD community in Bukit Duabelas National Park. However, data related to the spatial distribution of activity traces, harvesting and utilization of wild boar are still limited. Therefore, this study needs to be conducted to determine the spatial distribution of wild boar activity traces and identify the relationship between the harvesting and utilization of wild boar by Suku Anak Dalam (SAD) with respondents characteristics. The methods used in this study were direct observation, vegetation analysis, and in depth interviews with 40 respondents. The results showed that the types of wild boar activity traces observed in TNBD were feeding marks, body friction, fang marks, footprints, puddles and nests. The most common type of activity trace to be found is footprints with a total of 25 footprints. The spatial distribution of wild boar activity trace in TNBD is clustered (Ip>0). Wild boar harvesting by SAD is predominantly done during the day (45%) using modern and traditional techniques. Parts of wild boar that are being utilized is a total of 77%, and wild boar products were predominantly used by SAD in the form of consumption and trade.
Keyword: Suku Anak Dalam, Babi hutan, Taman Nasional Bukit Duabelas, Sebaran Spasial, Buspatial distribution, wild boar

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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