Judul: Karakterisasi Gen Odorant Receptor (OR) Parsial Serangga Penggerek Batang Padi Kuning Scirpophaga incertulas (Walker)
Abstrak: Yellow rice stem borer S. incertulas (Lepidoptera: Crambidae) is an important insect causes the damage of the rice crop. The females of S. incertulas naturally release sex pheromones and then captured by pheromone binding protein (PBP) in the haemolymph of male antennaes; subsequently sent to the odorant receptor proteins located on the olfactory sensory neuron dendrites on the antennae sensillae membrane which detect the chemical stimuli. The aim of this study was to characterize the odorant receptor (OR) gene that mediates the chemical information systems on the mating behavior of S. incertulas. RNA was extracted from male and female imago antennaes collected from Karawang and from rearing in Indonesian Centre for Agricultural Biotechnology and Genetic Resources Research and Development (BB-BIOGEN). The OR gene amplification from cDNA was performed by standard PCR, touchdown PCR and nested-touchdown PCR. The characterization of partial OR gene of S. incertulas male successfully performed by using touchdown-nested PCR. The OR partial gene sequence of S. incertulas revealed 133 bp size was homolog to the OR gene sequence of Ostrinia nubilalis (Lepidoptera: Crambidae) OnubOR7a (GenBank Accession No. AB597005.1). The homologous part showed that 133 bp sequence of S. incertulas was a candidate of OR genes that mediated the chemical information systems on the mating behavior of yellow rice stem borer. This study also successfully amplified beta-actin gene sequence 681 bp size from both male and female insects as the verification to the success of RNA extraction.
Keyword: 

Judul: Variasi Sensila pada Antena Ngengat Scirpophaga incertulas Walker (Lepidoptera: Crambidae)
Abstrak: Scirpophaga incertulas Walker (Lepidoptera: Crambidae) moth infests paddy, therefore it is known as yellow rice stem borer. The antenna of moth S. incertulas serve as the olfactory organ. The pores in antennal sensilla allow the feromon pass to the inner side of the antenna. The aim of this research was to study the diversity of antennal sensilla from the male and female S. incertulas by using scanning electron microscope (SEM). Goldplating method was applied for the purpose of coating process to increase the slide contrast. The antenna S. incertulas is filiform type and the antenna consists of three main parts, i.e. scape, pedicle, and flagellum. This study found that the S. incertulas male antenna has two types of sensila: trichodea sensilla (having three variations: TrI, TrII, and TrIII types) and bassiconica sensilla with two variations, BaII and BaIII types. Further in female antennal, the result of this study revelaed four types of sensilla: trichodea sensilla (TrI, TrII, and TRIII types), bassiconica sensilla (BaIII type), chaetica sensilla, and three variations of coeloconica sensilla (CoI, CoII, and CoIII types).
Keyword: Trichodea, Yellow rice stem borer, Sensila, Scanning Electron Microscope (SEM), Chaetica, Bassiconica

Judul: Formulasi Hamiltonian untuk Menggambarkan Gerak Gelombang Soliter Dimensi Tiga di Permukaan Laut
Abstrak: Gelombang permukaan adalah gelombang yang terjadi perbedaan rapat massa antara air dan udara. Salah satu gelombang permukaan yang ditinjau adalah gelombang soliter. Formulasi gelombang soliter dilakukan dengan menggunakan formulasi Hamiltonian.
Keyword: