Judul: Analisis Gaya Pengasuhan, Kecerdasan Emosional, Aktivitas Ekstrakurikuler, dan Prestasi Belajar Siswa di SMA Muhammadiyah Cirebon
Abstrak: Penelitian ini secara umum bertujuan untuk menganalisis hubungan gaya pengasuhan dengan kecerdasan emosional, kecerdasan emosional dengan aktivitas ekstrakurikuler dan prestasi belajar siswa Sekolah Menengah Atas. Tujuan khususnya adalah: (1) mengidentifikasi karakteristik contoh dan keluarga; (2) menganalisis keragaan gaya pengasuhan, kecerdasan emosional, aktivitas ekstrakurikuler, dan prestasi belajar; (3) menganalisis hubungan karakteristik contoh dan keluarga dengan gaya pengasuhan; (4) menganalisis hubungan gaya pengasuhan dengan kecerdasan emosional; (5) menganalisis hubungan kecerdasan emosional dengan aktivitas ekstrakurikuler dan prestasi belajar; (6) menganalisis hubungan aktivitas ekstrakurikuler dengan prestasi belajar.
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Judul: Hubungan pengasuhan dan kecerdasan emosi dengan prestasi belajar pada remaja
Abstrak: Secara umum penelitian ini bertujuan untuk mengetahui hubungan pengasuhan dan kecerdasan emosi dengan prestasi belajar remaja. Tujuan khusus penelitian ini adalah mengetahui (1) karateristik contoh yang terdiri dari umur, jenis kelamin, kegiatan ekstrakurikuler, kegiatan di luar sekolah dan karateristik keluarga yang terdiri dari pendidikan, pekerjaan dan pendapatan orang tua, serta besar keluarga; (2) pengasuhan dan kecerdasan emosi, pola dan fasilitas belajar serta prestasi belajar contoh; (3) hubungan antara karateristik keluarga dengan pengasuhan emosi contoh; (4) hubungan antara pengasuhan anak dengan kecerdasan emosi; (5) hubungan antara pengasuhan anak, kecerdasan emosi, pola dan fasilitas belajar dengan prestasi contoh. Penelitian ini menggunakan disain Cross Sectional Study. Penelitian ini dilakukan pada dua sekolah di Berastagi, Kabupaten Karo, Sumatera Utara. Kedua sekolah tersebut yaitu Sekolah Menengah Umum Negeri 1 dan SMA Masehi. Lokasi dan kedua sekolah tersebut dipilih secara purposive. Pengumpulan data dilakukan pada bulan April 2005. Contoh adalah remaja yang duduk di kelas dua SMA. Karateristik contoh dalam penelitian ini adalah remaja yang memiliki rangking 5 teratas dan 5 terbawah. Jumlah contoh dari kedua sekolah tersebut adalah 99 orang. Pada SMA Negeri 1, 70 orang dan 29 orang pada SMA Masehi. Pemilihan contoh tersebut dilakukan secara purposive. Jenis data yang dikumpulkan adalah data primer dan sekunder. Data di analisis secara deskriptif dan inferensial. Analisis statistik yang digunakan adalah uji Spearman dan Mann- Whitney. ...
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Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable