Judul: Uji kinerja spektrofotometer ultraviolet-tampak berkas ganda terhadap pengukuran ambroksol HCI pada tablet Eskpektoran
Abstrak: Performance test of double beam ultraviolet-visible (UV-Vis)Spectrophotometer on ambroxol HCI, measurement in expectorant table inculded two steps. The first test included wavelength accuracy, stray light, resolution, noise, baseline flatness,
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Judul: Pengembangan Spektrofotometer Sinar Tampak (KuantiVis) Berbasis Komponen Elektronik Umum dan Uji Kinerjanya
Abstrak: Spectrophotometer can be developed by replacing the main components with electronic components that are easy to find. This study aims to develop visible spectrophotometer (KuantiVis) device using light emitting diode lamp as light source, optical layer of digital versatile disks as grating, and web camera with charge-couple device sensor as detector. KuantiVis was tested for its performance based on linearity, limit of detection, limit of quantitation, and accuracy parameters using a standard of chromium. The optimum result showed that KuantiVis was made with dimensions of 33 × 16 × 5 cm and distance between web camera and source of light was 25 cm. The result showed that linearity of data based on R2 value was 0.9973, limit of detection was 0.1231 ppm, limit of quantitation was 0.4102 ppm, and recovery was 103.19%. The performance was not much different from the performance of Spectronic 20D+ and Genesys spectrophotometer. The KuantiVis was applied for determining borax concentration in wet noodles sample. Concentration of borax in sample was 28.60 μg/gram samples. The result of F test and t test showed that the data was not significantly different with that of Spectronic 20D+ and Genesys spectrophotometer.
Keyword: Bogor Agricultural University (IPB), visible spectrophotometer, light emitting diode, digital versatile disks, charge-couple device, borax

Judul: Mitochondrial DNA Gene Organization and Phylogenetic Relationship of Naja siamensis
Abstrak: Naja siamensis is one of Asiatic cobra in the Family of Elapidae, known as a cryptic snake. It has many morphological variations that lead to misidentification. Misidentification can cause the wrong concept in developing antivenom types due to high snakebite cases occur in Thailand. Thus, an accurate species identification using a molecular approach is crucially required. Mitochondrial DNA has been used widely in snake molecular studies. These studies were aimed to determine the arrangement of the N. siamensis partial mitochondrial genes using reptile-oriented primers and to reveal the phylogenetic relationship among other species of Asian cobra. DNA extraction was carried out based on the standard DNA salting-out protocol and was conducted by bioinformatics analysis using BLASTn and MEGA 6 software. Gene annotation was performed against N. kaouthia complete genome. A total of 3121 bp of N. siamensis mtDNA genes were sequenced consisting of partial of six regions: 16S rRNA, ND1, tRNA-Ile, CRII region, tRNA-Leu, tRNA-Gln, and COI genes. The mtDNA gene organization of N. siamensis was predicted to has a similar pattern with the common Elapidae mtDNA gene organization. This study showed that there is a monophyletic form in Naja genus and N. kaouthia is the closest species with N. siamensis based on partial 16S rRNA and ND1 gene.
Keyword: 16S rRNA, Elapidae, ND1, phylogenetic relationship