Judul: Modeling of Flood for Land Use Management (Case Study of Ciliwung Watershed)
Abstrak: Floods are one of the major disasters affecting many countries in the world year after year. It is an inevitable natural phenomenon occurring from time to time in all rivers and natural drainage systems. It causes damage to lives, natural resources and environment as well as the loss of economy and health. Floods represent complex problems because of its variety. Therefore, this variety cannot be studied or controlled only by one or two specific methods. The objectives of this research are to understand the process of flood events and its interaction with hydrometeorological components, to develop flood model for watershed management and to determine the effect of land use change to watershed discharge which indicates flood event. The research consists of four processes those are 1) Data Preparation, 2) Model Development, 3) Model Simulation and 4) Model Calibration and Validation. Data preparation was conducted for two kinds of data namely spatial data and tabular data. Model developed as numerical model of the hydrology of a river basin system. This model includes the response of watershed to precipitation, the actions of the river network as water flows through the river, the effect of land use changes, and the effect of engineering structures to the watershed. Model simulated by change land use as an input. Model calibrated by using water level data of field measurement in year 1996 and model validated by using water level data of field measurement in year 2000. The result of this reseach showed how the process based modeling is useful to model hidrological processes over the watershed. Precipitation is the main input for hidrology simulation and land use change gives the effect of water level in the watershed. The model which is built in this research can be used to evaluate runoff from different land use areas. For assessing the hydrological effects of land use changes on floods, three hypothetical scenarios, namely urbanization, deforestation and afforestation scenario, were considered made. It was found from the model simulation that the urbanization scenario has the higest negative impact on increasing water level. Deforestation has the second largest negative impact, while afforestation causes positive impact shown by decreasing water level. The result indicated that the urbanization produce the highest peak flow, followed by the deforestation and afforestation scenario. The simulated water level for the present land use is 84 cm, for the urbanization scenario 135 cm, deforestation scenario 92 cm, and afforestation scenario 59 cm. Accordingly, the urbanization scenario increases the peak discharge by 61%, the deforestation scenario increase 15%, and afforestation decrease by 19%.
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Judul: Optimasi penggunaan lahan dengan menggunakan model simulasi hidrologi daerah aliran sungai studi kasus di Sub DAS Ciliwung Hulu, Jawa Barat
Abstrak: The flood and drought that almost every year hit in Jakarta is related to the condition of Upper Ciliwung Sub Watershed as a recharge and protection area of Ciliwung Watershed. The high growth of population in the watershed caused increasing land needs and eventually decreasing forested land that supporting the function of recharge and protection of Upper Ciliwung Sub Watershed. Modelling is a way to find alternative measures for solving flood and drought problem in Jakarta. By using a model, behavior of river as the result of land uses changes can be observed without implemented in field, hence it save time and cost. Complicated and interrelated hydrologic processes as well as rapid land uses change requires a model to accomodate those factors with adequate accuracy. Beside using already available models, hidrologic parameter estimation in Upper Ciliwung Sub Watershed has also been done by using self-made model by using model builder software. Rozi (2002) has made a model by using STELLA. In his model, Rozi used monthly time unit that the model can’t be used to observe average daily debit that have potential contribution in causing flood in Jakarta.
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Judul: Peran Antibodi Kuning Telur (IgY) Sebagai Anti Adhesi dan Opsonin Untuk Pencegahan Serangan Mutan Streptococcus Serotipe d (Streptococcus Sobrinus)
Abstrak: Ayam petelur memiliki peran penting sebagai penghasil antibodi poliklonal. Antibodi ayam atau dikenal dengan imunoglobulin Y (IgY) dapat dipurifikasi dari kuning telur. Biaya untuk memproduksi IgY relatif lebih murah daripada antibodi mamalia karena biaya pemeliharaan ayam tidak mahal, relatif lebih mudah dan cepat menghasilkan telur. Penelitian ini bertujuan untuk mempelajari peran IgY spesifik MS serotipe d (Streptococcus sobrinus) (IgY-Ss) sebagai anti adhesi dan opsonin pada sel epitel pipi. IgY spesifik dikoleksi dari telur ayam Single Comb Brown Leghorn yang telah diimunisasi dengan antigen utuh S. sobrinus. Imunisasi diaplikasikan secara intravena dengan dosis antigen utuh S. sobrinus 0,5 ml (109CFU) selama tiga hari berturut-turut pada minggu pertama . Kemudian dilakukan pengulangan imunisasi (booster) sebanyak tiga kali dengan interval waktu seminggu secara intramuskular dengan dosis antigen utuh S. sobrinus 1 ml (109CFU) dalam Freud’s adjuvant complete pada minggu kedua dan dalam Freud’s adjuvant incomplete pada minggu ketiga dan keempat. Uji agar gel presipitasi (AGP) dilakukan untuk mengetahui terbentuknya IgY spesifik S.sobrinus (IgY-Ss). Ayam yang serum dan telurnya positif pada uji AGP kemudian telurnya di koleksi untuk dilakukan ekstraksi. Ekstraksi IgY-Ss dari kuning telur menggunakan metode PEG-amonium sulfat kemudian dipurifikasi dengan fast protein liquid chromatography. Konsentrasi IgY-Ss yang telah dimurnikan dihitung dengan spektrofotometer UV. Kemudian berat molekul IgYSs ditentukan dengan metode SDS Page dan menggunakan pewarnaan Commasie blue. Aktifitas biologis IgY-Ss sebagai anti adhesi dipelajari dengan assay adhesi secara in vitro menggunakan sel epitel pipi manusia. Assay adhesi dilakukan dengan dosis IgY-Ss 100 μg . Peran IgY-Ss sebagai opsonin dipelajari dengan assay fagositosis menggunakan dosis IgY sebesar 100 μg. Hasil penelitian yang diperoleh adalah IgY-Ss telah terbentuk dalam serum pada minggu kelima, sedangkan dalam telur pada minggu kesembilan setelah imunisasi pertama. Pre-inkubasi 100 μg IgY-Ss pada sel bakteri dan epitel pipi mampu menurunkan nilai adhesi dari 39,9 sel bakteri/ sel epitel pipi menjadi 30,7 sel bakteri/ sel epitel pipi. Opsonisasi dengan 100 μg IgY-Ss tidak meningkatkan aktifitas fagositosis namun hanya meningkatkan kapasitas fagositosis makrofag dari 1,6 sel bakteri/ makrofag menjadi 5,17 sel bakteri/ makrofag. Kesimpulan dari penelitian ini adalah ayam mampu memproduksi IgY spesifik anti S. sobrinus (IgY-Ss) pada serum dan kuning telur. Proses opsonisasi IgY-Ss pada S. sobrinus dapat meningkatkan kapasitas fagositosis. Preinkubasi IgY-Ss pada S. sobrinus (107 CFU/ ml) dan sel epitel pipi (105 sel) dapat mengurangi adhesi bakteri tersebut pada sel.
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