Judul: Mempelajari Pengaruh Intensitas Cahaya Terhadap Pertumbuhan Tunas Bibit Kentang ( Solanum tuberosum L. ) Selama Penyimpanan
Abstrak: Tanaman kentang sudah lama dikenal dan dibudidayakan di Indonesia, namun produksi rata-ratanya masih rendah yaitu sekitar 12 ton per hektar. Salah satu penyebab rendahnya produksi rata-rata adalah sulitnya mendapatkan bibit berkualitas tinggi di pasaran, selain jumlahnya terbatas, harganya pun cukup mahal, Dengan terbatasnya persediaan bibit kentang tersebut, maka diperlukan penanganan bibit kentang terutama penyimpanan. Selama penyimpanan berlangsung, umbi akan mengalami pertunasan. Untuk mendorong pertumbuhan tunas tanpa pemanjangan tunas yang berlebihan, maka penyimpanan pada tempat bertemperatur tinggi dapat dikombinasikan dengan pencahayaan. Pemberian intensitas cahaya di gudang penyimpanan tersebut tidak perlu berlebihan, karena intensitas cahaya yang berlebihan dapat menyebabkan perpanjangan tunas terhambat. Dengan demikian perlu mengetahui intensitas cahaya optimal untuk memeperoleh kualitas tunas yang baik
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Judul: Pengaruh jenis media preservasi dan lama penyimpanan gelap terhadap kemampuan tumbuh planlet kentang (Solanum tuberosum L.)
Abstrak: Penelitian dilaksanakan pada bulan November 1999 sampai September 2000, di Laboratorium Bioteknologi Biologi Molekuler dan Seluler, Pusat Penelitian Bioteknologi IPB. Tujuan dari penelitian ini adalah untuk mempelajari pengaruh jenis media preservasi dan lama penyimpanan gelap terhadap kemampun tumbuh planlet kentang. Percobaan menggunakan Rancangan Acak Lengkap faktorial dengan dua faktor, yaitu jenis media preservasi dan lama penyimpanan gelap. Jenis media preservasi terdiri dari atas 40 g/l sukrosa, 40 g/l sukrosa + 2 g/l hyponex, 40 g/l sukrosa + 18 mg/l spermidin, 40 g/l sukrosa + 2.5 mg/l PTS (Perak Thiosulfat), 40 g/l sukrosa + 2 g/l hyponex + 18 mg/l spermidin, 40 g/l sukrosa + 2 g/l hyponex + 2.5 mg/l PTS, 40 g/l sukrosa + 18 mg/l spermidin + 2.5 mg/l PTS dan 40 g/l sukrosa + 2 g/l hyponex + 18 mg/l spermidin +2.5 mg/l PTS. Lama penyimpanan gelap terdiri atas 0, 4, 8 dan 12 hari. Semua percobaan terdiri dari lima ulangan sehingga terdapat 8 x 4 x 5 = 160 satuan percobaan, setiap satuan percobaan terdiri dari empat planlet sehingga jumlah planlet yang digunakan pada percobaan ini sebanyak 640 planlet. Percobaan dilakukan sampai pada tahap aklimatisasi di rumah ketat serangga dengan menggunakan media arang sekam. Respon planlet yang diamati selama penyimpanan adalah pertambahan jumlah akar dan buku, sedangkan respon planlet yang diamati pada saat pra aklimatisasi di aboratorium dan aklimatisasi di rumah ketat serangga adalah pertambahan jumlah buku, pertambahan tinggi, persentase hidup dan lamanya waktu pemulihan (recovery periode). Setelah penyimpanan gelap planlet mengalami perubahan secara visual pada berbagai jenis media preservasi yang digunakan, perubahan yang ditimbulkannya adalah pemanjangan batang planlet, warna batang pucat, daun menguning dan mudah rontok serta planlet menjadi kurang tegar (lemas). Gejala- gejala yang ditimbulkan selama penyimpanan semakin tampak dengan semakin..dst
Keyword: planlet kentang, kultur jaringan, viabilitas, vigor bibit

Judul: The isolation and molecular identification of papillomavirus in macaca fascicularis and macaca nemestrina at animal facility of primate research centre – Bogor Agricultural University.
Abstrak: Cervical cancer is still regarded as a major cause of death in women world wide, hence research on papilloma viruses, their drugs and vaccines for the prevention are still continued. Papilloma virus is a DNA virus of the family papillomaviridae. Papilloma virions have no sheath, the diameter of 55 nm, and icosahedral capsid. The virus multiplies in the cell nucleus and causes chronic latent infection. The virus genome has a circular form, 8 kbp in length with 8 open reading frames. The genome is divided into early (E) and late (L) genes. The E gen synthesizes 6 E proteins, namely E1, E2, E4, E5, E6 and E7, which are linked into the process of viral replication and oncogenes. Meanwhile, the L gene synthesizes 2 proteins, the L1 and L2, which are related to the formation of the capsid (Hakim 2010). In order to obtain the best result of the cancer research, it is highly requested to obtain appropriate animal model that reflect aspects of human disease. Non-human primates have high similarity with humans, both in terms of the evolution of the genetic, anatomical, physiological, biochemical and organ systems, as well as in cancer genes. Non-human primates of the genus Macaca is a genus with the highest distribution worldwide, from Japan to Afghanistan. So far, there are 22 species are included in the genus Macaca. Originally rhesus monkeys or M. mulatta are widely used for biomedical research, but since the Indian government imposed a ban on importing these animals, then Macaca fascicularis (Long-tailed Macaque, cynomolgus) and Macaca nemestrina (Pig-tailed Macaque), which has high population in Southeast Asia, began to be used as research animal. Various medical aspects related to both species are commonly studied by many researchers as an animal model for human diseases. This study is using the polymerase chain reaction (PCR) method and nucleotide tracking to identify papillomavirus tipes that infect the genital tract of Macaca fascicularis and Macaca nemestrina, which is kept in breeding facilities of Primate Research Center, IPB. The analysis of nucleotide sequences were performed using the Clustal W 2.1 and 5.1 Mega program. The establishment of phylogenetic tree using neighbor joining with 1000 times bootstrap repetition. The results showed that the papillomavirus that infect Macaca fascicularis was 32.7% (78/238) and Macaca nemestrina 12% (4/31). Both species were infected by Macaca fascicularis papillomavirus (MfPV) tipe 3, 4, 5, 7 and 9, belongs to the genus Alpha papillomavirus with homology of 80 - 100%. Papilloma virus that infected the pig-tailed macaque was the same tipe of papillomavirus that infected the cynomolgus with similarity between 82% - 99% based on L1 region. To further explain the tipes of papilloma viruses that infected the pig-tailed macaque, it is necessary to identify the entire genome of the virus. To determine the tipe of the virus, it requires the identification of a more complete nucleotide sequence. It is interesting because papillomavirus that infected the pigtail macaques has 76% homology with the HPV tipe 52. This is the first report on the incidence of papillomavirus infection in the pigtail macaque genome that further analysis
Keyword: Papillomavirus, cervic cancer, animal model, Macaca fascicularis, Macaca nemestrina