Judul: Pengaruh Benzylaminopurine (BAP) dan Air Kelapa terhadap Laju Multiplikasi Stek Batang Nenas (Ananas comosus L. Merr) Klon Pasir Kuda-1
Abstrak: The research aimed to study the effects of Benzyladeninepurine (BAP) and coconut water by induces producing shoots from mother stems of pineapple (Ananas comosus L. Merr). The research was used Pasir Kuda-1 clone as planting materials. It was conducted at Pasir Kuda Research Farm at Bogor Agricultural University from January to June 2010. Method of this research was based on a completely block randomized design, consisted of one factor and three repetitions. The factor was the BAP concentration (0, 25, 50, 100 ppm) and coconut water. The stems of mother plant were sliced and soaked in solution of BAP and coconut water, and then they were raised in a husk charcoal media for several weeks until the shoots force out from lateral buds. These shoots were maintained in a secondary nursery until they reach the transplanting maturity. The shoots begin force out from lateral bud after two weeks and allowed to grow on stem cuttings until it has enough size for transplanting in secondary nursery. The data were analyzed by F test and significantly different results were tested further using Duncan's Multiple Range Test (DMRT) at 5% level. Variables observed at seeding stage are percentage of life cuttings, percentage of cuttings sprouted up, and shoots per cutting, while in the seedling stage are shoot high, shoot weight, leaf length, leaf width, and number of leaves. Harvesting was done gradually by four times and made observations of the quality of shoots produced at each harvest. The result showed that the treatment of BAP concentration and coconut water did not significantly affect to all of variables in primary nursery, such as percentage of live cuttings, percentage of cuttings sprouted up, and shoots per cuttings. Treatment also did not significantly affect in secondary nursery, except the number of leaves on shoots that are harvested first at 6 weeks after transplantation. The repetition based on planting material had significant effect on shoot height, leaf length, number of leaf, and weight of shoots. Time of harvesting also affected the quality of shoots produced. Harvesting with good quality of shoots was able until 16 week after plant period. The number of shoots height, shoots weight, leaf width, leaf length and leaf number decreased while the shoots harvested after those period.
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Judul: Pengaruh sitokinin (TDZ) dan auksin (IAA dan NAA) terhadap multiplikasi nenas, Ananas comosus L. Merr. cv. Queen dalam perbanyakan kultur jaringan
Abstrak: The objective of this research was to study the effect of Cytokinin (TDZ) and Auxin (IAA and NAA) as growth regulators in shooting multiplication capacity of shoot base pineapple explant. This research was conducted in tissue culture laboratory at Tropical Fruits Study Center. IPB. Baranangsiang. Bogor. since January until September 2004. Planting material of 5-10 mm base shoot of third subculture Queen pincapple's planlet were cultured in shooting induction medium consisted of MS suplemented with Auxin (IAA and NAA) and TDZ in various concentrations. The experiment was set up in factorial design with two factors, Auxin (5 levels: 0 mg/1. 0.1 mg/l IAA, 0.01 mg/l IAA, 0.1 mg/I NAA and 0.01 mg/l NAA) and TDZ (6 levels: 1x10 mg/l. 1x10 mg/l, 1x10 mg/l, 1x10 mg/l, 1x10 mg/l and 1x10 mg/l). There were 30 combinations treatment. Each treatment was consist of 4 replicates, containing 6 explants per replicate, so that found 720 unit. After 20 weeks in shooting induction medium, explants were transferred to MSO medium for shooting multiplication. Each treatment was consist of 12 replicates. In shooting induction phase, the percentage and the the number of shoot. root. nodule and chap nodule were examined. After subculture to MS free medium, the number of axillar shoot, adventitious shoot and chap nodule were examined every weeks. The weight of nodule were examined in the first and last week of observation. Meanwhile. the number of shoot per gram nodule weight were also examined in the last week of observation. The data were statistically analyzed using completely randomized design and the means were compared with Duncan's Multiple Range Test (DMRT) procedure at 5% level of significance. In the first week of the culture shoot induction medium. explants have shown its growing which marked with crosscut leaves elongation and new leaves formation. In almost treatments, shoot started to be formed at 2 weeks after culture (MST) and rooted at 3 MST. except for the treatments of TDZ at concentration 1x10 mg/l. which is combined with various Auxin concentrations. Explants grown in media supplemented with 1x10 mg/l. 1x105 mg/l, 1x10 mg/l. 1x10 mg/l TDZ in different concentrations of Auxin. initially responded by shoot and root formation with no nodules. There were little number of shoots (with amount of mean less than two) that formed in these culture medium by the end of perception week in both phases, shoot induction phase and shoot multiplication phase. Although the shoots cultured in media supplemented with low concentrations of TDZ (below 1x102 mg/l) were fewer in number. but the shoots were longer and have better quality than those formed in media supplemented with high level concentrations of TDZ (above 1x10 mg/) in the same perception week. Shoots became capable of rooting at combinations of 1x10- 1x10 mg/l TDZ with different concentrations of Auxin, but 1x10 mg/l without Auxin gave rise to the best rooting...dst
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Judul: Metrik Einstein (Anti-) Self-Dual Berdimensi Empat dengan Metode Cartan
Abstrak: Gravitational field equation can be formulated in differential forms (differential geometry), which describes gravity as a manifestation of the curvature of space-time. Curvature of space-time is characterized by a metric that satisfies the gravitational field equation. With differential forms it can be formulated the (anti-) self-dual Einstein equation, which is a modification of the Cartan structure in (anti-) self-dual basis. Calculation of (anti-) self-dual metric with (anti-) self-dual Einstein equation is simpler than the calculation using Christoffel symbols.
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