Judul: Rencana Pengelolaan Lanskap Pertanian Desa Pangulah Utara, Kecamatan Kota Baru, Kabupaten Karawang, Jawa Barat, sebagai Sarana Bermain Anak-Anak
Abstrak: Play is a need for children to learn how to socialize and develop their creativities. Agricultural landscape of Desa Pangulah Utara has a potential that could be developed as a children playground facility. The open space can be exploited as a mean to support play activities. The purpose of this research is to create a management plan of agricultural landscape of Desa Pangulah Utara, Kecamatan Kota Baru, Kabupaten Karawang, Jawa Barat, as a means of children playing and helps children to learn how to socialize and interact with their environment, also to introduce more about Sundanese traditional games. Data that used in this research consists of physical, biophysical, social, and managerial data. These data were analyzed descriptively, statistically, and by means of SWOT method. The results of SWOT analysis are six recomendation of management strategies of Desa Pangulah Utara as a mean of children playground: (1) providing the facilities that supporting children to play especially to play Sundanese traditional games, (2) providing play areas, (3) making the playing program that can increase the interest of children about Sundanese traditional games, (4) using local vegetation as materials of Sundanese traditional games, (5) making landscape management plan of agricultural landscape of Desa Pangulah Utara as children playground, and (6) specifying the areas that used as childreen playground without changing its function.
Keyword: traditional games, SWOT analysis, playing, management, agricultural landscape

Judul: Desain taman bermain berbasis perkembangan anak di Kecamatan Lengkong Kota Bandung
Abstrak: Persentase jumlah anak di Kelurahan Lingkar Selatan yaitu 25.8% dari jumlah keseluruhan penduduk. Kelurahan Lingkar selatan membutuhkan taman bermain agar anak dapat bermain secara aman dan sesuai dengan kebutuhan perkembangan anak. Taman lingkungan Lingkar Selatan dengan luas 4 750 m2 sesuai untuk dijadikan taman bermain karena letaknya berada di antara pemukiman warga. Tujuan dari penelitian ini adalah mendesain taman bermain anak yang sesuai dengan kebutuhan anak berbasis pada perkembangan dan pertumbuhan anak baik dari segi biologis, sosial, dan kognitif. Penelitian menggunakan metode deskriptif melalui survei, wawancara, dan kuisioner gambar. Konsep dasar yang diterapkan adalah asah,asih,asuh dengan penekanan pada peranan taman bermain sebagai area berkumpul keluarga dan tempat tumbuh kembang anak. Asih berhubungan dengan suasana kekeluargaan dalam taman. Asah berhubungan dengan aspek taman yang mampu meningkatkan kecerdasan anak baik itu secara intelektual maupun emosional. Asuh berkaitan dengan perawatan dan pemeliharaan anak sehingga anak selalu sehat secara fisik, mental, sosial, maupun spiritual. Konsep desain yang digunakan berasal dari gambar anak-anak tentang keluarga dengan bentukan dasar berupa lingkaran. Berdasarkan konsep desain yang ada, taman lingkungan Lingkar Selatan menjadi taman tematik yaitu Taman Keluarga. Zona taman terbagi menjadi tujuh ruang yaitu; zona umum (15.5%), zona remaja (5%), zona dewasa (5%), zona keluarga (45%), zona toddler (6%), zona preschool (11%), dan zona gradeschool (12.5%).
Keyword: Children’s development, playground, neighborhood park, landscape design

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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