Judul: Pemodelan Masa Belajar pada Usia Sekolah dengan Menggunakan Survival Analysis
Abstrak: Usia anak sekolah merupakan salah satu contoh data yang mengandung data tersensor, karena tidak semua responden dapat memberikan informasi yang lengkap mengenai kapan masa sekolah berakhir.
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Judul: Penyelesaian masalah data survival dengan menggunakan metode nonparametrik
Abstrak: Dalam kehidupan sehari-hari manusia tidak bisa lepas dari waktu. Banyak contoh-contoh kegiatan manusia yang berhubungan dengan waktu, seperti lamanya penyakit yang diderita seseorang hingga sembuh, jangka waktu lulusan suatu universitas hingga mendapatkan pekerjaan dan lain-lain. Data tentang pengamatan jangka waktu dari awal pengamatan sampai terjadinya suatu peristiwa (survival time) disebut data survival. Dalam data survival ada data tersensor ada juga data yang tidak tersensor. Untuk menganalisis dan mencari tingkat survival data-data tersebut diperlukan suatu metode yang sesuai. Untuk data tidak tersensor tingkat survival dari setiap individu dapat dicari dengan menggunakan persamaan : ^ ( ) j ij n t S t N ³ = yaitu rasio dari jumlah individu dengan tingkat survival ³ t dibagi jumlah individu dalam suatu himpunan. Untuk data tersensor dimana data tidak dapat diamati secara utuh, digunakan beberapa metode nonparametrik yaitu Life Table dan Kaplan Meier. Untuk membandingkan antar dua grup dalam survival data digunakan uji Logrank dan uji Wilcoxon. Contoh kasus yang dibahas adalah pengaruh jenis kelamin dan kandungan protein pada darah dalam menentukan tingkat survival pada pasien multiple myeloma.
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Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable