Judul: Aktivitas dan Stabilitas Superoksida Dismutase dari Ekstrak Deinococcus radiodurans yang Diimobilisasi pada Zeolit Alam sebagai Biosensor Antioksidan
Abstrak: Immobilization of superoxide dismutase (SOD) from extract of Deinococcus radiodurans have been studied. D. radiodurans is a bacteria having an enzyme Mn-SOD. The optimum condition of SOD antioxidant activity of the extract was at 31 °C, pH 9, and 157 mg zeolite. Determination of Michaelis-Menten constant was based on Lineweaver- Burk, Eadie-Hoofstee, and Hanes methods. The KMapp of SOD of the extract immobilized on zeolite was lower than that of without immobillization. Electrode stability was relatively stable up to 22 hours. Antioxidant activity of using zeolite as the matrix was higher than that of the unimmobilization. The antioxidant activity on electrode with zeolite at 22nd hour was 64.0 %, and the stability of electrodes without zeolite at the same time was only 49.3 %. The SOD of D. radiodurans is potential as a biosensor for antioxidants because of the high antioxidant activity despite of the low stability.
Keyword: superoxide dismutase, natural zeolites, Deinococcus radiodurans, antioxidant biosensor

Judul: Biosensor antioksidan berbasis superoksida dismutase deinoccus radiodurans diimobilisasi pada nanokomposit zeolit alam indonesia
Abstrak: The immobilization superoxide dismutase extract from D. radioduras on a zeolite nanocomposite modified electrode were studied. Cyclic voltammetry (CV) are employed to investigate the catalytic behavior of the biosensor. The superoxide radical was produced in solution using the xantine/xanthine oksidase system. Antioxidant activity vitamin C was determined by 2, 2-diphenil-2-picrylhydrazyl (DPPH) free radical scavenging method and comparing with biosensor SOD. Optimum conditions for SOD activity was at pH 9, temperature 300 C, zeolite 137.5mg, and SOD 3 U/ml for pure SOD and 9, temperature 300 C, zeolite 137.5mg, and SOD 1500μg/mL for D. radiodurans SOD. Dismutation reaction kinetics of superoxide catalyzed by SOD followed the Lineweaver-Burk kinetics with D. radiodurans SOD Kmapp value was higher than pure SOD. Antioxidant capacity for Vitamin C was 19.49 ppm using DPPH method and value 0,7 for relative antioxidant capacity using biosensor SOD. In conclusion, D radiodurans SOD immobilized on a zeolite nanocomposite had a great potential as biological recognition component for antioxidant biosensor.
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Judul: Closed Knight’s Tour pada Papan Catur Berbentuk Persegi Panjang dengan Ukuran Maksimal 8 × 8
Abstrak: Closed Knight’s Tour merupakan langkah kuda yang mengunjungi setiap kotak pada papan catur tepat satu kali dengan langkah kuda berawal dan berakhir pada kotak yang sama. Graf yang terbentuk dari closed knight’s tour dinotasikan sebagai graf G(m,n), dengan m adalah banyaknya baris dan n adalah banyaknya kolom. Graf tersebut memuat Hamiltonian cycle. Sebuah graf terhubung G adalah graf bipartite jika dan hanya jika graf tersebut tidak memiliki cycle dengan panjang ganjil. Papan catur yang memenuhi syarat cukup untuk memiliki closed knight’s tour adalah papan catur berukuran 5 × 6, 5 × 8, 6 × 6, 6 × 7, 6 × 8, 7 × 8, dan 8 × 8.
Keyword: bipartite, closed knight’s tour, hamiltonian cycle, knight’s tour, teori graf