Judul: Optical Reflectance Analysis of a Rice Field
Abstrak: Konsep dasar fisika dalam aplikasi penginderaan jauh seperti lidar merupakan suatu konsep dasar gelombang elektromagnetik yang memiliki hubungan antara kecepatan cahaya, panjang gelombang, dan frekuensi. Komponen tersebut biasanya digambarkan sebagai bentuk energi berjalan dalam gelombang sinosuidal. Panjang gelombang pada gelombang elektromagnetik dapat memperlihatkan pemantulan optik pada data lidar. Data lidar ini digunakan untuk menganalisis umur padi dan klasifikasi lahan sawah. Metode yang dilakukan pada penelitian ini yaitu menganalisis data lidar menggunakan perangkat lunak NEST dan Fugro-viewer. Klasifikasi lahan sawah dapat diketahui menggunakan pemantulan panjang gelombang dalam teori dasar fisika. Pada penelitian ini, daerah elektromagnetik dari panjang gelombang pada lahan sawah untuk semua kanal berada di daerah ultraviolet, daerah cahaya tampak, dan daerah inframerah dekat hingga tengah. Panjang gelombang dari setiap plot padi memiliki nilai sekitar 170-3580 nm berdasarkan semua kanal lidar di foto permukaannya.
Keyword: Pemantulan, Panjang gelombang, Padi, Data lidar

Judul: Pemantauan Pertumbuhan Padi Menggunakan L-Band SAR Berbasis Teori Dekomposisi: Studi Kasus Subang
Abstrak: Food problems in Indonesia cannot be avoided, although Indonesia is often referred to as an agricultural country where predominant livelihood is farming. As a matter of fact, there are still a lot of food shortages along increasing number of inhabitants. Rice is the main staple food in Indonesia, therefore monitoring rice field is important information for better agricultural planning. Monitoring with optical remote sensing would be on stake for Indonesia or tropical regions considering high cloud cover. SAR (Synthetic Aperture Radar) provides an alternative in the provision of data for such purposes, especially with polarization technology. As a system that has its own energy source, irrespective to source of solar energy, a SAR system can operate day and night in virtually all weather conditions, because microwaves can penetrate clouds, smoke and rain. This property is very useful for time sensitive applications such as sensing of agricultural crops or natural disasters. Earlier studies primarily focused on the use of backscatter data. Further development of the SAR technology allows analysis of fully polarization SAR data, which are generally more complex than backscattering-based analysis. Fully polarization SAR analysis procedures that are currently inviting attention is the polarimetric decomposition. This study discusses an application of the Cloude-Pottier decomposition theory in assessment of growth phase of Ciherang rice. Using this theory, a complex fully polarimetric data can be converted into three units of analysis, i.e. Entropy (H), Alpha Angle (􀄮) and Anisotropy (A). In this study, blocks of rice cultivation owned by PT. Sang Hyang Seri, provided major field information for detailed analysis. This research indicated that the Cloude-Pottier decomposition theory was able to describe characteristics of different levels of rice growth. The analysis showed that Entropy played an important role in distinguishing plants age. Distribution of entropy corresponded to various phases of rice growth. Aging rice plants tend to coincide to increasing value of entropy. Entropy became smaller in young rice plants, because of a decrease in the number of leaves or density of plant canopy and therefore soil contribution was dominant. Although the alpha angle was found useful to describe scattering type, this parameter is less visible to contribute significantly to the interpretation of data.
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Judul: Study of Tupaia javanica Hepatocytes Culture as In Vitro Replication Media of Nonhuman Primate HBV
Abstrak: Virus hepatitis B (VHB) adalah virus DNA yang menginfeksi organ hati sebagai target utama. Infeksi ini menyebabkan hepatitis yang dapat bersifat kronis dan berlanjut menjadi sirosis, kanker hati bahkan kematian. Pengembangan model in vivo dan in vitro berkaitan dengan hepatitis B menjadi perhatian khusus bagi para peneliti. Kultur hepatosit banyak digunakan untuk mempelajari berbagai ha] terkait hepatitis B baik dari aspek virus, penyakit, pencegahan, dan pengobatan. Pengembangan hepatosit asal hewan kecil sepe1ii Tupaia sp. untuk model in vitro merupakan salah satu altematif dalam penyediaan kultur hepatosit. Tujuan penelitian ini adalah mengembangkan kultur primer dan lestari hepatosit T. javanica yang mampu mendukung replikasi VHB orangutan (OuHBV) dan VHB owa jawa (GiHBV), dan dapat menjawab kebutuhan akan model in vitro kultur hepatosit. Isolasi hepatosit T. javanica dilakukan dengan prosedur sebagai berikut: organ hati yang telah dicacah dalam cawan petri dengan larutan perfusi, diinkubasi pada 37 °C. Suspensi disentrifugasi 300-500 g sehingga didapatkan pelet sel hati. Pelet yang sudah dicuci kemudian diresuspensi dengan Hepatocyte Basal Media untuk menumbuhkan hepatosit dan selanjutnya hepatosit akan ditransformasi menggunakan perangkat Lenti-SV 40. Sodium taurocholate cotransporting polypeptide (NTCP) yang diindikasikan sebagai reseptor hepatosit untuk melekatnya VHB, nampak pada hepatosit baik pada kultur primer maupun kultur yang ditransformasi. Reseptor hepatosit spesifik ini terlihat sebagai pendaran hijau dengan uji imunofluoresensi. Kultur hepatosit primer T. javanica berhasil dikembangkan selama 14 hari untuk mencapai kepadatan 80%, dan inokulasi GiHBV dan OuHBV ke dalam kultur pada hari ke-15 menunjukkan adanya replikasi virus hingga hari ke-8 (berdasarkan hasil PCR). Kuantifikasi virus dengan real-time PCR memperlihatkan replikasi tertinggi pada hari kedua setelah inokulasi. Penjajaran sebagian protein permukaan setelah virus dinokulasikan memperlihatkan kesamaan asam amino dengan virus wild type sebesar 99.29% dan 95.71 % pada GiHBV dan OuHBV., Hepatitis B virus (HBV) is a DNA virus which infects liver as primary target organ. This i11fection causes hepatitis which could become chronic and progress to cirrhosis, liver cancer and even death. Development of in vitro and in vivo model related to hepatitis B is a concern for researchers. Hepatocyte cultures widely used to study a variety of aspects of hepatitis B such as the virus, disease, prevention and treatment. Development qf hepatocyte from small animal e.g. Tupaia sp. for the in vitro model is an alternative of hepatocyte cultures. The purpose of the study is to develop primary and immortal T. javanica hepatocytes cultures capable to replicate orangutan HBV (OuHBV) and javan gibbon HBV (GiHBV), and to answer the need for in vitro model ofhepatocytes. Isolation of hepatocytes from T. javanica was done as followed: mincedliver were transported into petri dish containing perfusion buffer solution to be incubated at 37 °C. The suspension was centrifuged at 300-500 g to obtain liver cell pellets. The washed-pellet was resuspended in Hepatocyte Basal Media to let specific hepatocytes grow and later be transformed by Lenti-SV40 kit. Sodium taurocholate cotransporting polypeptide (NTCP) has been ident(fied as hepatocyte receptor for HBV attachment, and was seen on hepatocytes from both primary and transformed cultures. This hepatocytes specific receptor was shown as green luminenscent by immunofluorescence assay. Primary T. javanica hepatocytes cultures was successfully maintained for
Keyword: Tupaia javanica, in vitro model, hepatocytes culture, hepatitis B virus, Liver cancer, Picornaviridae, Hepadanaviridae, Flaviviridae, Caliciviridae