Judul: Analisis Transkriptomik Pohon Sengon (Falcataria moluccana Miq.) Rentan dan Resisten terhadap Hama Boktor (Xystrocera festiva Pascoe).
Abstrak: Sengon (Falcataria moluccana Miq.) plantations in monoculture system has increases boktor (Xystocera festiva Pascoe) pest population and distribution, however there is no effective and efficient control method yet. This study aims to analyze sengon transcriptomic data and identify expressed genes involved in sengon resistance to boktor pests, also other genes related to abiotic and biotic stresses. Unpublishe de novo transcriptomic data was acquired using the RNAseq and deposited in the DDBJ repository with accession number DRA008389. DEG analysis was perfomed using R program with edgeR package. The results of gene screening were then annotated using genes analog with the Swissprot database. DEG analysis produced 6859 upregulated contigs, genes in resistant tree were Heat shock cognate 70 kDA protein, Trypsin inhibitor, and Chalcone synthase. Based on previous research, trypsin inhibitor is a defensive mechanism of sengon against boktor pests, while other genes are known to be involved in the abiotic and biotic stress response.
Keyword: de novo transcriptomic, Falcataria moluccana Miq, trypsin inhibitor, Xystocera festiva Pascoe

Judul: Ekspresi Gen Trypsin Inhibitor Sengon (Falcataria moluccana Miq.) yang Sehat dan Terserang Hama Boktor
Abstrak: Sengon (Falcataria moluccana Miq.) merupakan salah satu jenis cepat tumbuh yang kayunya banyak dimanfaatkan sehingga sering dibudidayakan terutama di Pulau Jawa. Adanya serangan hama penggerek batang boktor (Xystrocera festiva Thombs) terhadap sengon menyebabkan kerugian yang besar karena dapat menurunkan kualitas kayu dan menurunkan nilai jual kayu sengon. Secara alami tanaman sengon memiliki gen ketahanan terhadap serangan hama boktor, salah satunya adalah trypsin inhibitor. Tujuan penelitian ini adalah membandingkan tingkat ekspresi gen trypsin inhibitor pada kambium pohon sengon resisten dan terserang hama boktor secara kuantitatif dengan teknik Quantitative Real Time PCR. Gen target yang digunakan yaitu trypsin inhibitor (TI) dan gen Actin sebagai internal kontrol dirancang dari analisa data transkriptom sengon. Data Threshold cycle (CT) yang diperoleh kemudian dikuantifikasi menggunakan metode comparative CT method (2 -ΔΔCT). Hasil kuantifikasi menunjukkan tingkat ekspresi gen trypsin inhibitor pada ketiga sampel sengon resisten yaitu sebesar 1.90, 2.20, dan 2,85 sedangkan sengon terserang boktor sebesar 4.07, 2.05, dan 4.08. Rata-rata ekspresi gen trypsin inhibitor pada sengon terserang hama boktor lebih tinggi dibandingkan sengon yang resisten. Kata kunci: Falcataria moluccana, Trypsin Inhibitor, Quantitative Real Time PCR, Sengon (Falcataria moluccana Miq.) is highly demanded fast growing tree species which leads to the increasing sengon cultivation especially in Java. Boktor pest (Xystrocera festiva Thombs) decrease the quality of sengon wood, causing a high loss due to lower selling price of the log. Naturally sengon has resistance gene to defend itself from boktor pest attacks, and one of these genes is trypsin inhibitor. This study aimed to comparing the level of trypsin inhibitor gene expression in sengon’s cambium that is resistant and attacked by boktor pests. The analysis of gene expression level was carried out using the Real Time PCR technique. Primers of trypsin inhibitor (TI) gene as the target gene and Actin gene as the internal control were designed from sengon’s transcriptomic data analysis. Threshold cycle (CT) that obtained then quantified using comparative CT method (2 -ΔΔCT). Quantification results showed that the expression level of trypsin inhibitor from three resistant samples are 1.90, 2.20, and 2,85 while in sengon attacked by boktor are 4.07, 2.05, and 4,08. The average trypsin inhibitor gene expression in sengon attacked by boktor pest was higher than resistant ones. Keywords: Falcataria moluccana, Trypsin Inhibitor, Quantitative Real Time PCR
Keyword: Falcataria moluccana, Trypsin Inhibitor, Real Time PCR

Judul: Metrik Einstein (Anti-) Self-Dual Berdimensi Empat dengan Metode Cartan
Abstrak: Gravitational field equation can be formulated in differential forms (differential geometry), which describes gravity as a manifestation of the curvature of space-time. Curvature of space-time is characterized by a metric that satisfies the gravitational field equation. With differential forms it can be formulated the (anti-) self-dual Einstein equation, which is a modification of the Cartan structure in (anti-) self-dual basis. Calculation of (anti-) self-dual metric with (anti-) self-dual Einstein equation is simpler than the calculation using Christoffel symbols.
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