Judul: Penerapan metode Two Step Cluster untuk Mengklasifikasikan Desa-desa di Kabupaten Madiun Berdasarkan Data Potensi Desa
Abstrak: Pembangunan Desa merupakan bagian mendasar untuk pembangunan Nasional. Membangun desa membutuhkan informasi tentang apa yang dibutuhkan oleh masyarakat. Penelitian ini bertujuan untuk mengelompokkan desa-desa di Kabupaten Madiun yang memiliki persamaan karakteristik terhadap satu sama lain dan mengidentifikasi karakteristik gerombol-gerombol yang terbentuk. Sehingga, permasalahan khusus di dalam gerombol-gerombol desa dapat dijadikan dasar untuk melaksanakan pembangunan desa. Metode yang digunakan untuk amatan dengan peubah campuran adalah two-step cluster. Peubah yang digunakan dalam penelitian ini adalah 14 peubah terdiri dari enam peubah kategorik dan delapan peubah numerik. Analisis gerombol menghasilkan 4 kelompok. Kelompok yang memerlukan perhatian lebih untuk dikembangkan adalah kelompok 2 yang memiliki nilai terendah dalam segi sumber daya dan fasilitas. Koefisien Silhouette untuk kluster yang terbentuk adalah 0.3 yang dapat dikategorikan pada kategori fair.
Keyword: analisis gerombol, koefisien Silhouette, two-step cluster

Judul: Penerapan Metodetwo Step Cluster Dalam  Analisis Gerombol  (Studi Kasus : Data Potensi Desa Sensus Ekonomi 2003 Wilayah Jawa Barat)
Abstrak: Clustering is a process of grouping objects based on similarities . Some problems in cluster analysis are the different scale of measurement, large data sets and unspecified number of cluster. One approach to handle this problems is by using Two Step cluster method. The purpose of this research is to applicate Two Step cluster method by clustering villages residing in West Java. The result of Two Step cluster method are pre-cluster which early yielded at first phase counted eight clusters and optimal cluster which yielded at second phase counted three clusters. Members in first cluster are objects which is extreme outliers and cannot be grouped into other clusters. Villages which include into second clusters have rural characteristic. The village has wide farm, many agriculture households, but also has underdeveloped field of industries and also information and communications. So that, to improve village potency for this cluster, the variables which still have low growth level has to be increased. The third cluster has the characteristic of urban status. Village in this cluster has the closest distance to downtown, developed enough in the field of industry, information and communications, but still have the highest number for unemployment citizens.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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