Judul: Pengaruh Gaya Pengasuhan Orang Tua, Kecerdasan Emosional, Dan Kecerdasan Kognitif Terhadap Prestasi Belajar Remaja SMu
Abstrak: Remaja yang berkualitas adalah remaja yang memiliki keunggulan dari segi fisik dan mentalnya. Kualitas sumberdaya manusia tercermin dalam kecerdasan emosional dan kecerdasan kognitif. Kedua kecerdasan tersebut bersama-sama akan mempengaruhi prestasi belajar remaja dan menentukan keberhasilan dalam hidup.
Keyword: 

Judul: Pengaruh Praktek Pengasuhan dan Iklim Sekolah terhadap Kecerdasan Emosi Remaja SMK.
Abstrak: Saat ini, mengembangkan kecerdasan emosi remaja belum menjadi fokus perhatian keluarga dan sekolah padahal kecerdasan emosi akan sangat menentukan kualitas hidup remaja di masa depan. Keluarga melalui praktek pengasuhan memiliki peran penting dalam membentuk kecerdasan emosi. Selain itu, iklim sekolah juga turut memengaruhi terbentuknya kecerdasan emosi yang baik pada remaja. Penelitian ini bertujuan untuk menganalisis pengaruh karakteristik remaja dan keluarga, praktek pengasuhan, dan iklim sekolah terhadap kecerdasan emosi remaja. Penelitian ini melibatkan 175 siswa sekolah menengah kejuruan. Lokasi penelitian dipilih secara purposive di wilayah Kota Bekasi, Jawa Barat. Hasil penelitian menunjukkan tidak ada perbedaan yang signifikan dalam hal kecerdasan emosional remaja antara anak laki-laki dan perempuan. Hasil uji hubungan menunjukkan variabel praktek pengasuhan dan iklim sekolah berhubungan erat dengan kecerdasan emosi remaja dan secara konsisten memberikan pengaruh nyata berdasarkan hasil uji regresi. Pihak keluarga dan sekolah yang mampu meningkatkan kualitas praktek pengasuhan dan meningkatkan iklim sekolah yang lebih baik akan menyebabkan remaja cerdas secara emosi. Untuk penelitian lebih lanjut, direkomendasikan untuk membuat perbandingan antara daerah pedesaan dan perkotaan dengan melibatkan tidak hanya remaja pada siswa sekolah menengah atas tetapi juga remaja awal dan remaja akhir.
Keyword: kecerdasan emosi, lingkungan sekolah, praktek pengasuhan, remaja

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable