Judul: Pendekatan Metode Hidroakustik Untuk Analisis Keterkaitan Antara Tipe Substrat Dasar Perairan Dengan Komunitas Demersal
Abstrak: Substrat dasar perairan berperan penting bagi kehidupan biota yang hidup di daerah dasar perairan. Dasar perairan adalah habitat bagi bentos, ikan demersal dan juga biota laut lainnya. Oleh karena itu, akurasi dan kecermatan yang tinggi untuk mengklasifikasikan substrat dasar menjadi penting. Namun data mengenai tipe dasar perairan dan hubungannya dengan biota, khususnya ikan demersal masih sangat kurang. Hal ini disebabkan metode yang dipergunakan secara umum terbatas pada metode grab yang hanya mampu memetakan areal yang sempit berdasarkan stasiun sampling di dasar perairan.
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Judul: Pendekatan metode hidroakustik untuk analisis keterkaitan antara tipe substrat dasar perairan dengan komunitas ikan demersal, The hydroacoustic method approach for interrelatedness analysis of sea bottom substrate type with demersal fish community
Abstrak: The sea bottom substrate has an important role for biota life in sea bottom area. Sea bottom is habitat for benthic, demersal fishes and other marine biota. Therefore, the accuracy and precision to classify the sea bottom substrate become essential. However, data and information on sea bottom substrate type and its relation to biota, particularly demersal fishes, are scanty. This condition is caused by the used method, in general, is limited to grab method which is only covered and mapped a very limited area based on sea bottom sampling station. Nowadays, the new method to obtain sea bottom substrate information has been developed very well, that is hydroacoustic method. Sea bottom substrate, sediments, benthic and vegetations can be accessed by using echosounder and processed digitally. The difference of sea bottom substrate types can be distinguished through the coarseness of topography and hardness of sea bottom substrate which consisted of coral, stone, sand, mud, and clay. The research objective is to analyze the interrelatedness of sea bottom substrate type with demersal fish community by hydroacoustic method approach. Therefore, the obtained data consisted of sea bottom substrate, demersal fish, oceanographic data, and other related data. Those data were obtained from several locations, i.e. Belitung Island in the years of 2002 and 2005, Java Sea (2002 and 2005), East Borneo (2004), and around Pari Island of Seribu Islands (2007). Data processing was done by using several echo data post processing programs, namely Simrad EP-500, Echoview version 3.5 and Simrad BI-60. The hydroacoustic data of sea bottom substrates and grab sampling results were classified based on Principal Component Analysis (PCA) and Cluster Analysis. The relation between the abiotic factors and demersal fish community was overlaid descriptively over horizontal distribution map of sea bottom substrate and demersal fish species by using surfer program. The result of bottom sounding shows that the harder the sea bottom substrate, the stronger the backscattering value. On the opposite, the softer the sea bottom substrate, the weaker the backscattering value. Based on PCA and cluster analysis, it is known that strongest backscattering strength value of sea bottom substrate is from coral substrate, followed by backscattering values from sand, muddy sand, sandy mud, and mud, which is weaker respectively. The obtained sea bottom substrate backscattering values can be applied to assess and distinguish sea bottom substrate type in other sea bottom area. The hydroacoustic detection of demersal fishes and swept area by trawl give information of fish density higher at shallow water (<36,5 m) and have small value of fish target strength. These small fish was identified as Leiognathus splenden (“ikan pepetek”). The relation between abiotic factors and demersal fish community in Java Sea shows that: 1) Fish species of Leiognathus splenden does not live in all sea bottom with muddy substrate. This fish was found in the muddy area in a shallow water near coastal zone. 2) Fish species of Upeneus sulphureus has high appearance frequency at all trawl swept area stations. This fish was encountered in a muddy substrate and the total weight of fish (swept by trawl) is gradually higher
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Judul: Gene Editing  of Two Cyanide Synthesis-related Genes in Cassava using CRISPR/Cas9
Abstrak: Cassava (Manihot esculenta) contain toxic quantities of cyanogenic glucoside. Reducing the potentially toxic cyanogenic glucoside levels in cassava could be achieved by gene editing through Clustered Regularly Interspaced Short Palindromic Repeats – CRISPR associated protein 9 (CRISPR/Cas9), a high- precision genome editing technique in plants. Two cassava UDP- glucosyltransferase (UGT) paralogs, UGT85K4 and UGT85K5, are the genes catalyzing the synthesis of cyanogenic glucosides in plants. The study aimed to construct the CRISPR/Cas9 recombinant vector carrying single guide RNA (sgRNA) to edit UGT85K4 and UGT85K5 genes, and transform sgRNA CRISPR/Cas9 construct into KU50 cassava calli. Five sgRNAs were designed, considering the on/off target efficiency score and GC content. Those sgRNAs were ligated into pRGEB32 plasmid and introduced to competent Escherichia coli DH5a. The PCR colony and DNA sequencing were performed to verify the sgRNA insert. The amplicons showed the expected band size of recombinant vector (180 bp). Based on sequencing results, only one sgRNA was successfully ligated into the plasmid that will be used to edit the UGT85K5 gene. The sgRNA CRISPR/Cas9 construct in pRGEB32 plasmid was successfully transformed into Agrobacterium tumefaciens and introduced into KU50 cassava calli through co-cultivation. The overgrowth of Agrobacterium occurred after the co-cultivation phase.
Keyword: CRISPR/Cas9, Cyanogenic glucoside, Gene editing, sgRNA