Judul: Analisis Molekuler dan Evaluasi Umur Berbunga Tanaman Padi Putatif Transgenik AtCO
Abstrak: The timing of flowering is an important character which impact yield and quality in crop plant. The flowering time control is best understood in the model Arabidopsis (Arabidopsis thaliana). Recent result from rice plant research to be related with Arabidopsis thaliana reported that flowering control is manage with fotoperiodism. Insertion of flowering gene control was done on Nipponbare rice plant to hasten of flowering time with transfer CONSTANS gene. The aim of research are evaluation and analys response transgenic rice plant putative AtCO for flowering time, and testing genes existence transcriptional factor which is responsible on flowering time use PCR method. PCR analysis use spesific primer and DNA genom as template, meanwhile for expression analysis of AtCO gene on transcriptional level done by isolation total RNA. cDNA sintesis doing by reverse transcription-PCR (RT-PCR) and amplification AtCO gene product with cDNA as a template. Result of this research showed that out of 80 transgenic plants putative AtCO there are 35 positive plants containing AtCO gene. Flowering time evaluation for 35 rice plants positive AtCO showed earlier flowering time are during 63 until 67 days than their wildtype plant are 68 days. Expression analysis of AtCO gene on 5 plants showed that only one plant expressed AtCO gene that indicated by PCR product from cDNA template.
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Judul: Transformasi Genetika Padi Indica dengan Gen OsMADS18 Menggunakan Agrobacterium Tumefaciens untuk Merakit Varietas Padi Umur Pendek
Abstrak: Rice is the staple food of more than half the world's human population. The need of rice each year continues to increase with increasing population. This study aimed to assemble a short-lived rice varieties by introducing OsMADS18 gene into the genome of plants using Agrobacterium tumefaciens. OsMADS18 gene is a short age gene which has been successfully constructed to pCAMBIA1301 vector under CaMV35S constitutive promoter. This is evidenced by the emergence of 12561 bp DNA band size, this measure is a combination among vector pCAMBIA 1301 (11819 bp) and OsMADS18 genes (742 bp). Then the vector pCAMBIA 1301 that contains OsMADS18, was used to transform Agrobacterium tumefaciens strain EHA105 through Heat-shock method. The success of transformation marked by the growth of the white colonies Agrobacterium tumefaciens on solid media LB, this media contained antibiotic, X-gal and IPTG. Then the bacteria are used to transforms 50 calli Indica rice by soaking the calli into colonies for 20 minutes. The calli moved to selection media that contains hygromycin. After 14 days, calli that resistant to hygromycin moved to regeneration media R3B grown for transgenic plant. 18 transformants plantlets were obtained. Gus-A successful test on the show with the appearance of blue color in leaf tissues. While PCR analysis of the 18 transgenic plantlets produced 742 bp sized bands.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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