Judul: Marketing Strategy and Business  Development Model for Green Bean Kelompok Tani AMANAT in Nanggung Village,  Bogor Regency
Abstrak: Kelompok Tani AMANAT merupakan kelompok tani kopi robusta yang berada di Desa Nanggung. Proyek ini bertujuan memetakan model bisnis kopi, merumuskan faktor internal dan eksternal yang berpengaruh pada pengembangan bisnis, dan menetapkan prioritas strategi pemasaran dan model pengembangan bisnis yang baru untuk diterapkan. Proyek ini melalui 5 tahapan yaitu hasil eksplorasi dan pendefinisian masalah, verifikasi masalah, ideasi, pengembangan prototipe, dan validasi. Alat analisis yang digunakan yaitu bussiness model canvas (BMC), kuisioner preferensi pelanggan, matriks internal factor evaluation (IFE), matriks external factor evaluation (EFE), matriks internal-external (IE), matriks strength weakness opportunity dan threat (SWOT), serta quantitative strategic planning matrix (QSPM). BMC menggambarkan secara komprehensif elemen-elemen yang terlibat dalam bisnisnya sehingga dapat mengetahui elemen business model canvas yang belum optimal. Preferensi pelanggan bertujuan mengetahui karakteristik green bean yang dibutuhkan. Berdasarkan matriks IE Kelompok Tani AMANAT berada dalam kuadran II, yaitu Grow and Build Strategy. Strategi umum yang direkomendasikan pada kuadran ini adalah penetrasi pasar, pengembangan pasar, pengembangan produk, dan integrasi ke depan. Matriks SWOT menghasilkan alternatif strategi yang merujuk pada strategi umum, dilanjutkan menentukan prioritas strategi dengan QSPM. Prioritas strategi utama adalah memperluas area pemasaran dengan green bean berkualitas. BMC baru menunjukkan 3 elemen utama perubahan yaitu value propositions, customer segments, dan key activities., Kelompok Tani AMANAT is a robusta coffee farmer group in Nanggung Village. This project aims to map the coffee business model, formulate internal and external factors that influence business development, and set new marketing strategy priorities and business development models to be implemented. This project went through 5 stages, namely the results of exploration and problem definition, problem verification, ideation, prototype development, and validation. The analytical tools used are the business model canvas (BMC), customer preference questionnaire, internal factor evaluation (IFE) matrix, external factor evaluation (EFE) matrix, internal-external (IE) matrix, strength weakness opportunity and threat (SWOT) matrix, and quantitative strategic planning matrix (QSPM). BMC comprehensively describes the elements involved in its business so that it can find out which elements of the business model canvas are not optimal. Customer preferences aim to determine the required characteristics of green beans. Based on the IE matrix, Kelompok Tani AMANAT is in quadrant II, namely Grow and Build Strategy. The general strategies recommended in this quadrant are market penetration, market development, product development, and forward integration. The SWOT matrix produces alternative strategies that refer to the general strategy, followed by determining strategic priorities with the QSPM. The main strategic priority is to expand the marketing area with quality green beans. The new BMC shows 3 main elements of change, namely value propositions, customer segments, and key activities.
Keyword: customer preferences, business model, farmer groups, strategic  alternatives, strategic priorities

Judul: Design of Coffee Processing Improvement as an Effort to Increase the Quality of Green Bean Robusta Farmer Group AMANAT Nanggung Village
Abstrak: Peningkatan kualitas produk merupakan salah satu upaya dalam keberlanjutan agroindustri kopi rakyat. Kelompok Tani AMANAT Desa Nanggung terpilih sebagai usaha kopi tani yang potensial untuk dikembangkan. Proses pengolahan kopi oleh petani Kelompok Tani AMANAT Desa Nanggung masih menghasilkan green bean yang belum memenuhi standar SNI dan termasuk kategori mutu asalan sehingga perlu dilakukan perbaikan. Metode yang digunakan terdiri dari eksplorasi, pendefinisian masalah, ideasi, pengembangan prototipe dan validasi. Hasil dari penelitian berupa prototipe desain proses pengolahan pascapanen metode natural yang sudah diperbaiki tahapannya dan proses pengolahan metode honey. Perbaikan yang dilakukan yaitu dengan penambahan proses sortasi basah, perpanjangan lama waktu pengeringan, dan pemaksimalan proses sortasi kering. Berdasarkan hasil penelitian, green bean hasil perbaikan metode natural dan metode honey dapat memenuhi syarat mutu umum SNI Biji Kopi dengan kategori mutu grade 1., Improving product quality is one of the efforts in the sustainability of the people's coffee agro-industry. The AMANAT Farmer Group in Nanggung Village was selected as a potential farming coffee business to be developed. The coffee processing by AMANAT Farmer Group farmers in Nanggung Village still produces green bean that does not meet SNI standards and belongs to the category of random quality, so improvements need to be made. The method used consists of exploration, problem definition, ideation, prototype development and validation. The results of the research are in the form of a design prototype for post-harvest processing using the natural method which has improved its stages and the processing using the honey method. Improvements made are by adding a wet sorting process, extending the drying time, and maximizing the dry sorting process. Based on the results of the research, green beans resulting from the improvement of the natural method and the honey method can meet the general quality requirements of SNI Coffee Beans with grade 1 quality category.
Keyword: Green bean, post harvest, processing, quality, SNI

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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