Judul: Pengaruh Penambahan Ekstrak Etanol Temu Ireng (Curcuma Aeruginosa Roxb.) Terhadap Pertumbuhan Sel Lestari Tumor Mca-B1 Dan Mcm-B2 Secara In Vitro
Abstrak: Penelitian ini bertujuan untuk mempelajari kemampuan penghambatan ekstrak etanol temu ireng terhadap pertumbuhan sel lestari tumor MCA-B1 dan MCM-B2. Penelitian dilakukan dengan menanam sel lestari tumor MCA-B1 dan MCM-B2 pada tissue culture plate 24 well dengan konsentrasi ekstrak yang berbeda sebanyak 3 kali ulangan. Konsentrasi ekstrak yang dipakai adalah 0 ppm sebagai kontrol negatif (tidak diberi penambahan ekstrak), doxorubicin 100 ppm sebagai kontrol positif, 12.5 ppm, 25 ppm, 37.5 ppm, 50 ppm. Setelah sel lestari tumor pada lubang kontrol negatif konfluens, sel dipanen dan dihitung menggunakan kamar hitung hemositometer Neubauer dengan pewarnaan trypan blue. Sel lestari diinkubasi pada suhu 37oC, 5% CO2. Hasil penelitian menunjukkan adanya aktivitas antiproliferasi ekstrak etanol temu ireng yang berbeda nyata p(<0.05) terhadap kedua sel lestari tumor MCA-B1 dan MCM-B2. Dosis ekstrak yang menunjukkan hasil paling baik adalah 50 ppm dengan aktivitas antiproliferasi sebesar 80.59% pada sel lestari tumor MCA-B1 dan 84.33% pada sel lestari tumor MCM-B2. Hasil tersebut menunjukkan potensi temu ireng sebagai tanaman yang memiliki aktivitas sebagai anti tumor.
Keyword: antiproliferasi, ekstrak temu ireng, MCA-B1, MCM-B2, sel lestari

Judul: Efek Pemberian Ekstrak Etanol Temu Ireng (Curcuma Aeruginosa Roxb.) pada Proliferasi Sel Lestari Tumor THP1 dan A549 Secara In Vitro.
Abstrak: The use of plant extracts is one of alternative ways to heal tumor diseases. The purpose of this research is to study the effect of ethanol extract of temu ireng (Curcuma aeruginosa Roxb.) on proliferation of THP1 and A549 cell lines. The extract concentration used were 0 ppm (as a negative control), 12.5 ppm, 25 ppm; 37.5 ppm and 50 ppm, doxorubicin as a positive control, and the treatment were in three replications. All data were analyzed statistically by ANOVA and Duncan test. The results showed that inhibition activity of temu ireng ethanol extract on THP1 and A549 cell line proliferation were significantly different (p<0.05) than negative control. Inhibition activity were as higher as treatment dose and significantly different (p<0.05) between dose, except 25 ppm and 37.5 ppm dose on THP1 cell line. The highest inhibition was achieved at 50 ppm dose as 85,8% for THP1 cell line and 73.3% for A549 cell line. Based on this results, we concluded that temu ireng ethanol extract has a poliferation inhibition activity on both cell lines and has a potential substance to be develop as antitumor.
Keyword: A549, curcuma aeruginosa Roxb, inhibition activity, THP1

Judul: Characteristic of Overshooting Top (OT) Based on  Himawari  8  Satellite  as  a  Convective  Cloud  Feature  that  Generates  Extreme Weather in the Java Island Region
Abstrak: Overshooting Top (OT) is a feature at the top of a convective cloud that is associated with extreme weather. Research on OT in tropical Indonesia is still very limited. The existence of geostationary satellites with wide area coverage and fast frequency observations is useful for observing the fast dynamics of the OT life cycle. Therefore, this study intends to analyze the relationship between OT and extreme weather, updraft and thermodynamic conditions of OT when extreme weather occurs, the best objective method for detecting OT in the day time and night time, and the spatiotemporal distribution of OT in the tropical region of Java Island during the 2018-2021 period. Based on visual analysis using visible channel and Himawari 8 sandwich products, 87% of extreme weather events in Java were accompanied by the appearance of OT. It indicates that the presence of the OT pattern is a convection cloud pattern or feature that is closely related to extreme weather events for the Java Island region. The existence of a time lag between the appearance of OT and extreme weather events on Java Island indicates that the OT of convection cloud can be used as a precursor or nowcasting for the appearance of extreme weather. Most of the convective cloud OT features during extreme weather events on Java Island have altitude that were only able to exceed the altitude of the equilibrium level and few were able to exceed the tropopause level of 9 km – 16 km. The difference in atmospheric conditions during extreme weather events on Java Island, which is in the tropics and subtropics, is a strong and unstable convective energy condition characterized by a larger CAPE, as well as unstable KI, LI, SI and TTI values. It is offset by kinematic factors in the form of a weak vertical wind shear (bulk wind shear 0-6 km) and a higher freezing point height. The difference between extreme weather events in the Java Island region and other tropical regions compared to subtropical regions is the strong updraft speed of convection clouds. The IRW Texture COMB algorithm based on the IRW-Texture Gradient modification and the COMB algorithm shows a significant increase in quality for detecting OT and non-OT pixels in Java. Rainy days with rainfall > 50 mm/day are very common in areas with an altitude of > 500 m. On the other hand, the level of convection activity which is indicated by the presence of OT and lightning is significantly reduced in this area. OT of convective clouds appear more frequently during the DJF season and less frequently during the JJA season. OT often appears during the day time and rarely at night time. Most OTs appear in the central part, away from the south and north coasts of Java Island during the day, while OTs that appear near the south and north coasts of Java Island can appear in the morning or afternoon.
Keyword: convective clouds, extreme weather, Overshooting Tops, Himawari 8 Satellite