Judul: Studi Sistem Pengadaan Tanaman Lanskap di Kelurahan Bojongsari Baru, Kecamatan Bojongsari, Kota Depok, Jawa Barat
Abstrak: Plants are important elements in landscape design so that nurseryman are also required in landscape industry besides landscape architects, landscape contractors and landscape managers. This research was conducted in Bojongsari Baru village which is one of the business centers of ornamental plants, in July-December 2013. The purpose of this study was to study the procurement system of small-scale nurseries run by Bojongsari Baru citizens and to compose some strategies for ornamental plant business development. Research methods with observation and interviews of citizens then performed descriptive and quantitative analysis to get an overview of plant business conditions and SWOT analysis for business development strategy. The results of this study indicate cultivation, plant maintenance, and marketing is still done using conventional methods and simple tools due to the limited availability of agricultural land and capital, but citizens have a desire to develop this business. Several strategies recommended for nurserymen in Bojongsari Baru are careful production planning, optimization of land use, and more detailed financial records.
Keyword: ornamental plant., nurseryman, nursery, landscape

Judul: Study of Landscape Plant Nurseries Procurement in Sukamantri Village Tamansari Sub-district Bogor Regency
Abstrak: Pengusaha tanaman lanskap memiliki peran penting dalam industri lanskap dengan memproduksi dan menyediakan tanaman yang sesuai spesifikasi arsitek lanskap. Permintaan tanaman lanskap yang fluktuatif menyebabkan produksi dan penyediaan yang fluktuatif. Tidak sesuainya jenis tanaman yang disediakan dengan permintaan konsumen menyebabkan stok tanaman yang memiliki permintaan rendah menumpuk di nurseri. Desa Sukamantri merupakan salah satu sentra produksi dan penjualan tanaman lanskap di Kabupaten Bogor yang menyediakan tanaman lanskap bagi konsumen di dalam negeri dan di luar negeri. Oleh karena itu, perlu dilakukan kajian untuk mendapatkan rekomendasi strategis terkait pengadaan tanaman di Desa Sukamantri agar nurseri dapat berkembang lebih baik dalam hal pengadaan tanaman dan berkelanjutan. Penelitian dilaksanakan di Desa Sukamantri dari bulan November 2022 sampai dengan Maret 2023. Data dianalisis secara kualitatif dengan pendekatan SWOT terkait faktor internal dan eksternal pengadaan tanaman lanskap. Hasil penelitian menunjukkan pengadaan tanaman masih menggunakan metode konvensional namun telah berkontribusi dalam memenuhi permintaan pasar dalam negeri maupun luar negeri. Beberapa strategi yang direkomendasikan dalam pengembangan sistem pengadaan tanaman lanskap ialah perluasan pasar dengan melakukan promosi di media sosial, pengembangan keterampilan sumber daya manusia, pengoptimalan penggunaan lahan dan teknologi yang tersedia serta perencanaan produksi tanaman lanskap agar sesuai kebutuhan pasar., Nurseryman play an important role in the landscape industry by producing and supplying plants. The fluctuating demand for landscape plants leads to fluctuating production and supply. The mismatch of the types of plants provided with consumer demand causes the stock of plants that have low demand accumulate in the nurseries. Sukamantri Village is one of the centers of production and sales of landscape plants in Bogor Regency that provides landscape plants for consumers in domestic and foreign market. It is necessary to conduct a study to obtain strategic recommendations related to plant procurement in Sukamantri Village so that the nurseries can develop better in plant procurement and sustainability. The research was conducted in Sukamantri Village from November 2022 to March 2023. Data were analyzed qualitatively with a SWOT approach related to internal and external factors of the landscape plant procurement. The results showed that the plant procurement still uses conventional methods but has contributed to fulfilling domestic and foreign market demands. Some of the recommended strategies in developing the landscape plant procurement system are market expansion by conducting promotions, developing human resource skills, optimizing the use of available land and technology and planning landscape plant production to suit market needs.
Keyword: Analisis SWOT, Nurseri, Pengusaha tanaman lanskap, Tanaman lanskap

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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