Judul: Penambatan Molekul Flavonoid pada Propolis dengan Enzim Histon Asetiltransferase sebagai Antikanker.
Abstrak: Histon asetiltransferase p300/CBP-associated factor (HAT PCAF) merupakan enzim yang berperan mengkatalisis pemindahan gugus asetil dari asetil-KoA ke asam amino ɛ-lisin pada histon inti. Salah satu target HAT PCAF ialah p53 yang berfungsi dalam memperbaiki DNA dan menginisiasi apoptosis sel, namun kondisi stress in vitro mengakibatkan asetilasi p53 berlebih oleh HAT PCAF yang dapat memicu terjadinya kanker. Penelitian ini bertujuan menguji potensi dan mekanisme penghambatan HAT PCAF oleh flavonoid propolis, yaitu apigenin, galangin, krisin, kuersetin, pinobanksin, pinokembrin, dan tektokrisin. Penelitian dilakukan dengan menggunakan metode penambatan molekul. Hasil penelitian menunjukkan asetilasi histon oleh HAT PCAF dapat dihambat oleh flavonoid propolis. Flavonoid tersebut mampu menghambat pengikatan asetil- KoA, histon, ataupun asetil-KoA dan histon oleh HAT PCAF. Penghambatan yang dilakukan memiliki afinitas yang baik ditunjukkan dengan energi afinitas yang rendah.
Keyword: HAT PCAF, flavonoid, propolis

Judul: Kinetika Inhibisi Dan Analisis In Silico Molekuler Kurkumin Kunyit (Curcuma Longa) Terhadap Histon Asetiltransferase
Abstrak: Kurkumin kunyit (C. longa) sudah diketahui mampu menghambat Histon Asetiltransferase (HAT) p300/CBP associated factor (PCAF) sebagai biomarker kanker. Namun, kinetika inhibisi dan mekanisme molekuler inhibisi PCAF HAT belum diketahui. Oleh karena itu, penelitian ini bertujuan untuk mengetahui kinetika inhibisi dan menganalisis secara molekuler PCAF HAT melalui in silico. Pada kinetika, kurva standar diukur dari larutan yang mengandung KoA 0- 800 pmol dengan total reaksi 100 μL, yang direaksikan dengan 7-Diethylamino-3- (4’-Maleimidylphenyl)-4-Methylcoumarin) (CPM). Uji kinetika menggunakan bufer HEPES, pH 7.5, BSA, dan konsentrasi substrat (H3) secara tepat (20±10 μL), dan masing-masing enzim (PCAF) ditambahkan ke dalam reaksi dengan total reaksi 100 μL, termasuk 10 μL isopropanol dan 5 μL CPM dalam reaksi. Mikroplate diukur dengan OPTIMA MARS flourescence microplate reader dengan pengaturan gain 65 pada gelombang emisi dan eksitasi 482 dan 392 nm. Data dimasukkan dalam plot Dixon. Pada docking molekuler, Kurkumin digambar secara manual menggunakan marvin sketch 6.0. Struktur kristal PCAF HAT diunduh dari Protein Data Bank (kode 1CM0). Simulasi molekuler docking menggunakan aplikasi AutoDock Vina 1.5.6. Visualisasi hasil simulasi menggunakan Ligplot+ 1.5.4. Pembanding data menggunakan uniprot. Hasil penelitian menunjukkan kurkumin dapat menghambat PCAF HAT dengan tipe inhibisi uncompetitive. Hal tersebut didukung dengan analisa molekuler docking in silico yang menunjukkan bahwa kurkumin mengikat residu asam amino yang diprediksi mampu mengubah konformasi enzim-substrat pada proses katalitiknya. Hasil penelitian ini dapat disimpulkan bahwa kurkumin dapat menjadi inhibitor yang spesifik terhadap PCAF HAT.
Keyword: docking, HAT, kinetika, kurkumin

Judul: Biology and ecology of the buffalo fly, haematobia exigua de meijere (diptera:muscidae)
Abstrak: The life history of the buffalo fly was observed at room temperature of 27°-29.5°C and at a relative humidity of 48-68 percent. The eggs hatched after 17-22 hours, with an average of 18 hours. First instar larvae measured 1.25 + 0.16 mm long and 0.27 + 0.037 mm wide. reach the second larval stage. It took 6-12 hours to the second instar larvae measured 2.676 +0.187 mm long and 0.353 +0.028 mm wide. The second instar took about 12-24 hours to reach the third larval stage. The third instar measured 6.833 + 0.189 mm long and 0.919 + 0.051 mm wide. It took about 2-3 days for the third instar to become pupae. Duration of the pupal stage was about 5-7 days before the adults emerged. Under laboratory condition, the buffalo fly completed its life cycle in 9.5 to 10.5 days, whereas under field condition it took 9-14 days with an average of 10.5 days. The growth ratio which was based on the measurements of the cephalo-pharyngeal skeleton of each instar was 1.797. The preoviposition period was 8-12 days and mating usually occurred on the 6th or 7th day. The longevity of the male was 29 days, whereas it was 32 days in the female. The range of egg production under laboratory condition was between 20-139 per female fly. The belly and hind legs of the carabao were the most preferred habitat either during cloudy and cool dry or shiny and warm day. When the weather was clear and the carabao was in the shade, the flies tended to be more distributed particularly on the shoulder, dewlap, back and horns. The carabaos had relatively fewer Musca sp. and Stomoxys calcitrans than cattle; never the less carabaos had a higher infestation of buffalo fly than cattle. Oviposition occured either during the day or night but the buffalo flies were more active during the night than in the day time. The effect of various lengths of exposure time of manure in relation to the oviposition was profound. observed, the maximum number of the buffalo flies was occurred when the dung was exposed for 30 minutes. With increasing time of exposure the number and variety of insects also increase. It was evident that Sepsidae and psychodidae were the most dominant insects emerging from the dung. The number of buffalo flies also increased with increasing
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