Judul: Kesesuaian Ruang Terbuka Hijau pada Rencana Tata Ruang Wilayah dan Tutupan Lahan di Kota Bekasi
Abstrak: Changes in land cover in Bekasi City, which grew rapidly resulting in a potential unsuitable between the Spatial Planning (RTRW) with existing land cover. The purpose of this study was (1) To analyze changes in land cover classification results using Landsat 7 ETM + and Landsat 8 OLI in Bekasi City in 2003, 2009 and 2013, (2) To analyze the suitability of green open space (RTH) on theland cover with the Spatial Plan (RTRW) of Bekasi City. The method used was the digital image analysis using supervised classification method, and the thematic change analysis using geographic information system The results showed that the suitability of green open space on Spatial Planning and land cover between 2003, 2009 and 2013 indicated the most appropriate RTH between Spatial Planning with land cover was in 2009 in the amount of 312.54 Ha while the most unsuitable was the RTH of 2003 in the amount of 129.89 ha
Keyword: Bogor Agricultural University (IPB), Bekasi City, green open spaces, cover change

Judul: Kesesuaian Ruang Terbuka Hijau pada Pola Ruang Menggunakan Citra SPOT-7 di Kecamatan Ciomas dan Tamansari Kabupaten Bogor
Abstrak: Pertumbuhan penduduk menyebabkan penggunaan lahan untuk permukiman dan lahan terbuka yang berdampak pada penurunan luas ruang terbuka hijau (RTH) di Kecamatan Ciomas dan Tamansari. Penelitian ini bertujuan menganalisis perubahan RTH menggunakan citra SPOT-7 tahun 2016 dan 2021 dan menganalisis kesesuaiannya dengan rencana tata ruang wilayah (RTRW) Kabupaten Bogor tahun 2016-2036. Luasan RTH pada tahun 2016 hingga 2021 di Kecamatan Ciomas dan Tamansari mengalami penurunan, tetapi luasan RTH tiap kecamatan masih memenuhi peraturan yang telah ditetapkan oleh pemerintah pada daerah perkotaan yaitu sebesar 30%. Kesesuaian RTH dengan RTRW di Kecamatan Ciomas didapat mencapai 64,04% pada tahun 2016 dan meningkat menjadi 67,33% pada tahun 2021, sedangkan di Kecamatan Tamansari nilai kesesuaian RTH dengan RTRW lebih tinggi sebesar 93,19% pada tahun 2016 namun turun menjadi 89,40% pada tahun 2021., Population growth causes the use of land for settlements and open land which has an impact on reducing the area of green open space (RTH) in Ciomas and Tamansari districts. This study aims to analyze changes in green open space using SPOT-7 imagery for 2016 and 2021 and analyze their suitability with the Bogor Regency regional spatial plan (RTRW) for 2016-2036. The area of green open space in 2016 to 2021 in the Ciomas and Tamansari districts has decreased, but the area of green open space for each district still meets the regulations set by the government in urban areas, namely 30%. The suitability of green open space with RTRW in Ciomas district reached 64,04% in 2016 and increased to 67,33% in 2021, while in Tamansari was higher at 93,19% in 2016 but decreased to 89,40% in 2021.
Keyword: spatial pattern, SPOT imagery, Bogor Regency Spatial Planning, green open space, land cover

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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