Judul: Study on the Effect of Vegetation on Thermal Comfort in City Parks in Central Bogor Sub-district
Abstrak: Taman kota merupakan salah satu jenis ruang terbuka hijau publik yang dapat dimanfaatkan sebagai ruang terbuka hijau. Vegetasi pada suatu taman dapat membuat lingkungan terasa nyaman karena kehadirannya dapat memperindah dan mengubah iklim mikro pada kawasan tersebut. Taman Kencana dan Taman Malabar merupakan taman terluas di Kecamatan Bogor Tengah dengan luas masing-masing 4796 m2 dan 5518 m2. Tujuan penelitian ini adalah untuk mengidentifikasi faktor-faktor yang mempengaruhi kenyamanan termal dan rekomendasi konsep vegetasi untuk meningkatkan kenyamanan termal taman kota dan sekitarnya di Kecamatan Bogor Tengah. Penelitian dilakukan dengan metode perencanaan Simonds yang terdiri dari tahapan persiapan, penelitian/pengumpulan data, sintesis, dan perencanaan dengan menggunakan metode analisis data deskriptif kuantitatif. Hasil penelitian menunjukkan bahwa terdapat korelasi yang signifikan antara suhu permukaan dan suhu udara di Taman Kencana dan Taman Malabar dengan nilai koefisien korelasi r = 0,781 dan r = 0,849 secara berurutan. Besaran pengaruh luas vegetasi dan lahan terbangun secara simultan di Taman Kencana terhadap LST (Land Surface Temperature), suhu udara, RH (Relative Humidity), dan THI (Temperature Humidity Index) secara berurutan adalah sebesar 70%; 64,4%; 43,6%; dan 62,9%. Besaran pengaruh luas vegetasi dan lahan terbangun secara simultan di Taman Malabar terhadap LST, suhu udara, RH, dan THI adalah sebesar 65,7%; 67,1%; 13,5%; dan 71%. Luas vegetasi berpengaruh secara signifikan terhadap LST, suhu udara, RH, dan THI di kedua taman. Pada Taman Kencana, 69,7% LST dipengaruhi oleh luas vegetasi, 64% suhu udara dipengaruhi oleh luas vegetasi, 43,3% RH dipengaruhi oleh luas vegetasi, dan 61,8% THI dipengaruhi oleh luas vegetasi. Pada Taman Malabar, 65,2% LST dipengaruhi oleh luas vegetasi, 66,2% suhu udara dipengaruhi oleh luas vegetasi, 9% RH dipengaruhi oleh luas vegetasi, dan 71% THI dipengaruhi oleh luas vegetasi. Luas vegetasi yang harus dioptimalkan dalam satu grid secara efektif untuk mencapai kenyamanan termal ideal di Taman Kencana dan Taman Malabar dengan nilai THI 28,6 secara berurutan yaitu sebanyak 366 m2 dan 349 m2 terutama pada grid area prioritas. Area yang menjadi prioritas untuk diterapkan vegetasi meliputi area parkir, plaza, pathway, pekarangan, dan rooftop., City parks are open public spaces that offer various recreational activities. The presence of greenery in a park can create a comfortable environment and enhance its beauty by altering the microclimate of the area. Kencana Park and Malabar Park are the two biggest parks in Central Bogor Sub-district, with areas covering 4796 m2 and 5518 m2, respectively. The objective of this study is to identify the factors that impact thermal comfort in these parks and suggest vegetation concepts that can enhance the thermal comfort of city parks in Central Bogor Sub-district. The research conducted used the Simonds planning method, which involves preparation stages, data collection/research, synthesis, and planning using quantitative descriptive data analysis techniques. The research findings indicate a significant correlation between surface temperature and air temperature in Kencana Park and Malabar Park with correlation coefficient values of r=0,781 and r=0,849, respectively. In Kencana Park, the vegetation area and built-up land have a 70%, 64,4%, 43,6%, and 62,9% influence on Land Surface Temperature (LST), air temperature, Relative Humidity (RH), and Temperature Humidity Index (THI), respectively. In Malabar Park, the vegetation area and built-up
Keyword: thermal comfort, urban park, vegetation

Judul: Thermal Comfort by Differences Land Cover around Bogor Botanical Garden Area
Abstrak: Peningkatan jumlah penduduk di Kota Bogor mengakibatkan kebutuhan akan lahan terbangun yang semakin meningkat. Hal ini akan memengaruhi unsur iklim dan kondisi kenyamanan. Penelitian ini bertujuan untuk memperoleh informasi tingkat kenyamanan termal di wilayah sekitar Kebun Raya Bogor secara spasial dan temporal menggunakan pendekatan Universal Thermal Climate Index (UTCI) serta mengidentifikasi pengaruh perubahan tutupan lahan. Nilai UTCI diperoleh dari hasil observasi lapang yaitu dengan melakukan pengukuran data meteorologi dan menganalisis hasil kuisioner di delapan titik lokasi yang merepresentasikan pemukiman, lahan terbangun, jalan dengan vegetasi jarang, jalan bervegetasi rapat, lahan bervegetasi jarang, dan lahan bervegetasi rapat. Hasil yang diperoleh dari penelitian ini menunjukkan rataan nilai UTCI musiman berada pada kategori moderate heat stress hingga very strong heat stress. Tutupan lahan bervegetasi memiliki suhu udara yang lebih rendah dibandingkan tutupan lahan lainnya. Nilai persepsi panas di sekitar wilayah Kebun Raya Bogor dalam kesetaraan suhu °C, menunjukkan nilai dominasi dingin saat UTCI ≤ 30,6 dengan kategori moderate heat stress. Seluruh responden menyatakan panas jika nilai UTCI mencapai ≥ 40,5 dengan kategori UTCI very strong heat stress. Penelitian ini mendapatkah kondisi kawasan di sekitar Kebun Raya Bogor tergolong cukup nyaman dengan skor kenyamanan 0,7. Faktor-faktor yang dapat meningkatkan persepsi panas di sekitar wilayah Kebun Raya Bogor adalah nilai UTCI, durasi di lokasi, preferensi suhu, MET, dan ragam pakaian, sedangkan faktor-faktor yang secara nyata memengaruhi persepsi nyaman adalah nilai UTCI dan preferensi suhu., The increasing number of residents in the city of Bogor has resulted in an increasing need for built-up land. This will affect the climatic elements and comfort conditions. This study aims to obtain information on the level of thermal comfort in the area around the Bogor Botanical Gardens spatially and temporally using the Universal Thermal Climate Index (UTCI) approach and identify the effect of land cover changes. The UTCI value was obtained from the results of field observations, namely by measuring meteorological data and analyzing the results of questionnaires at eight location points representing settlements, built-up land, roads with sparse vegetation, roads with dense vegetation, sparsely vegetated land, and densely vegetated land. The results obtained from this study indicate that the average of seasonal UTCI value is in the category of moderate heat stress to very strong heat stress. Vegetated land cover has a lower air temperature than other land cover. The value of heat perception around the Bogor Botanical Gardens area is equal to temperature °C, indicating the value of cold dominance when UTCI 30.6 in the moderate heat stress category. All respondents stated that it was hot if the UTCI value reached 40.5 with the UTCI category of very strong heat stress. This study found that the condition of the area around the Bogor Botanical Gardens was quite comfortable with a comfort score of 0.7. Factors that can increase the perception of heat around the Bogor Botanical Gardens area are UTCI value, duration in location, temperature preference, MET, and variety of clothing, while the factors that significantly affect comfortable perception are UTCI value and temperature preference.
Keyword: Kebun Raya Bogor, kenyamanan termal, kerapatan vegetasi, tutupan lahan, UTCI

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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