Judul: Viabilitas Parasit Ichtyophthirius multifiliis yang Dipelihara pada Suhu Rendah
Abstrak: The ciliate Ichthyophthirius multifiliis, an obligat fish parasite, was maintained at low temperature as attempt to prolong its life span and reduce the number of fish hosts required in its laboratory propagation. The experiments were conducted using trophont stage of parasite which originally isolated from any outbreaks of white spot disease. Briefly, parasites were subjected at optimal temperature 25- 28°C (control) and at low temperature 9°C for 14 consecutive days of observation. The survival rate, encystation rate, excystation rate, and the abnormality of descendants which produced by maintained parasites were examined at the last day of observation. Here the infectivity of parasites then performed by means of infecting the animal model either black moly Poecilia sphenops or red belly pacu Colossoma macropomum with escaping theronts which collected during excystation. The results revealed that the survival rate and the excystation rate of parasites were decreased, while the encystation rate and the abnormality increased as maintaining period increased. Spesifically, the final rate of survival, encystation, and excystation of parasites were 35%, 100%, and 33,33% respectively. Additionally, the descendants came out with highly abnormality recognized by their weak mobility and their lower infectivity (40-50%) compared to the control (80-100%). Overall, from the study concluded that, it is possible to maintain I. multifiliis at low temperature without its host for 14 days, where it still gain its infectivity, eventhough being decreased.
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Judul: Provision of vaccine effectiveness against Ichthyophthirius multifiliis common carp (Cyprinus carpio L) at different temperatures of media
Abstrak: The aims of this study were to assess the physiological and haematological responses of fish due to different water temperatures, Ichthyophthirius multifiliis (ich) infection, and vaccination with ich theront stadia. The research was conducted in three stages. The first stage was designed to determine the temperature range that could cause stress in fish and had potency to cause ich infection. In the second stage, fish was infected with ich. The last stage was carried out to investigate the effect of vaccination. Physiological changes in fish from each stage were determined. The results of the first stage showed that cortisol levels at 20, 24, and 28⁰C and glucose levels at 24 and 28oC increased until 21st day. Fish that was kept at 24oC had the lowest survival rate (87±3.35%) and fish that was kept at 32oC had the highest survival rate (100%). In the second stage, infected fish at 20oC was under great stress, which was characterized by the increase in cortisol levels by 51.38% on 3rd day, increase in glucose levels by 60.93%, decrease in osmolarity by 18.40%, decrease lymphocytes percentage by 16.44%, and the lowest survival rate (58.11±2.16%). The damage that was affected by I. multifiliis could be seen by the presence of hypercellular, many goblet cells, and infiltration of lymphocytes cells. Lastly, vaccination caused the decrease in cortisol levels and increase in glucose levels until 14th day. The prevalence of I. multifiliis in the vaccinated fish decreased with the increase in temperature, and I. multifiliis prevalence at 20⁰C was 80.03±10.00% and at 28⁰C was 0%, and the highest survival rate was obtained at 28⁰C (100%).
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Judul: Desain Lanskap Jalan Slamet Riyadi Kota Surakarta Berbasis Budaya Jawa.
Abstrak: Kota Surakarta dianggap sebagai salah satu pusat kebudayaan Jawa. Keberadaan benda cagar budaya serta warisan kesenian dan kebudayaan menjadi potensi Kota Surakarta untuk membangun karakteristik sebagai kota budaya. Penataan ruang bernuansakan budaya di Kota Surakarta dirasa perlu, terutama ditengah pesatnya modernisasi pembangunan yang terjadi saat ini. Jalan Slamet Riyadi merupakan salah satu area yang berpotensi untuk dikembangkan dengan nuansa budaya Jawa. Penelitian ini bertujuan untuk mengidentifikasi warisan budaya yang ada di jalan Slamet Riyadi, mengidentifikasi dan menganalisis potensi yang ada pada lanskap jalan Slamet Riyadi, dan mendesain lanskap jalan Slamet Riyadi dengan berbasis budaya Jawa. Penelitian ini mengacu pada proses perancangan Gold 1980 dengan modifikasi. Konsep desain yang digunakan pada penelitian ini adalah konsep tata ruang rumah bangsawan Jawa pada jaman dahulu atau delem yang terdiri dari pelataran, pendhapa, pringgitan, dan dalem ageng. Kemudian bagian-bagian tersebut diterapkan sebagai sub-tema desain setiap segmen pada tapak dan menjadi gambaran dari zona-zona penyusun tapak. Desain dilakukan pada area Citywalk, jalur sepeda, badan jalan dan beberapa ruang di sekitar tapak. Desain ini juga mengambil beberapa bentuk ragam hias bangunan Keraton Kasunanan dan Mangkunegaran serta motif batik khas Keraton Kasunanan dan Mangkunegaran. Hasil penelitian ini berupa siteplan, detail view, gambar potongan, gambar ilustrasi dan detail desain.
Keyword: Citywalk, Dalem, Desain Berbasis Budaya Jawa, Desain Lanskap Jalan, Kota Budaya