Judul: Analisis pola agroforestri tradisional di Distrik Kokas Kabupaten Fakfak Propinsi Papua Barat
Abstrak: Penelitian ini dilaksanakan selama satu bulan yaitu, pada bulan Mei 2012 di Distrik Kokas, Kabupaten Fakfak, Propinsi Papua Barat. Responden yang diwawancara sebanyak 37 orang dari dua Desa yaitu Desa Kiriwas-was dan Desa Kinam. Pemilihan responden pada tiap desa contoh dilakukan dengan cara purposive, dengan sasaran para petani yang memiliki kebun agroforestri tradisional. Metode pengumpulan data dengan teknik wawancara, observasi, dan pengumpulan data sekunder. Pengolahan dan analisis data dilakukan dengan cara reduksi, penyajian data, dan penarikan kesimpulan. Kebun yang dimiliki oleh responden berasal dari hutan alam yang kemudian dibuka dan dijadikan kebun. Hutan alam yang dibuka dibagi berdasarkan marga dan pada umumnya kebun yang dimiliki responden merupakan harta warisan (tanah milik). Pengelolaan kebun terbagi atas kegiatan persiapan lahan, persiapan bibit, penanaman, pemeliharaan, dan pemanenan yang masih bersifat sederhana. Pola tanam kebun adalah pola dengan kombinasi antara kayu, buah-buahan, dan tanaman pertanian. Tanaman yang dipilih merupakan jenis yang tidak terlalu banyak membutuhkan perawatan, perhatian khusus, dan merupakan jenis andalan setempat seperti pohon pala. Kebun memberikan kontribusi yang besar terhadap pendapatan petani. Hal ini dapat dilihat dari rata-rata kontribusi kebun terhadap pendapatan total petani yaitu sebesar 53,5%. Kendala pengelolaan yang dihadapi oleh petani umumnya adalah adanya gangguan binatang liar seperti babi hutan, rusa, dan binatang hutan lainnya.
Keyword: Bogor Agricultural University, Institut Pertanian Bogor, IPB, Traditional agroforestri, Local knowledge, Garden, Contribution of income

Judul: Analisis Pola Agroforestri Tradisional di Distrik Kokas Kabupaten Fakfak Propinsi Papua Barat
Abstrak: One method of cultivating the forest in Fakfak Regency is the practice of traditional agroforesty system known as garden system. The garden managemen It is based on the existing local knowledge and was produced to substance needs. It is necessary to study the management and the benefits from this agroforestry garden. This study was conducted in one month, on May 2012, and located in the province of West Papua, Fakfak Regency, Kokas District. Targeting farmers who have gardens (traditional agroforestri). Respondets interviewed were as many as 37 people in two villages, namely the villages of Kiriwas-was and Kinam. The respondents in each village were selected by a purposive sampling method. The data were collected from secondary data as well by interview and observation. The data were processed and analyzed with a method of reduction, presentation, and conclusion. The gardens owned by the respondents came from natural forests, which were then opened and made into gardens. Natural forests are opened and divided among the clans; and the respondents‟ gardens were in general inherited property (land). Garden cultivation is divided into several simple (traditional) activities such as land preparation, seed preparation, planting, maintenance, and harvesting. The results of the field study showed that the most common planting pattern (agroforestri) was the combination of wood, fruits and plantation crops. The crops selected were the types that do not require too much care, attention, and of local mainstay for example, nutmeg (Pala). These garden gave so many contribution to farmers income. It can be seen from the average contribution to the total income from the farmers garden, that is equal to 53,5%. The problem faced by the average farmers from the three tribes is the intrusion of animals such as wild boar, deer and other forest animals.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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