Judul: Aktivitas enzim selulase isolat asal Indonesia pada berbagai substrat limbah pertanian
Abstrak: Isolat C11-1 dan KBM-2 memiliki aktivitas enzim selulase yang relatif tinggi dibandingkan isolat lain pada substrat selulosa CMC Sigma, Avicel dan Kertas saring Whatman no. 1. Hal ini menggambarkan enzim ekstrak kasarnya mengandung endo-1,4-â-glukanase dan ekso-1,4-â-glukanase. Kemampuan enzim selulase isolat C11-1 dan KBM-2 dalam mendegradasi substrat limbah pertanian juga tinggi. Isolat C11-1 memiliki aktivitas enzim selulase tertinggi pada substrat jerami padi sebesar 179 x 10-3 nkat/ml, sedangkan KBM-2 memiliki aktivitas enzim selulase tertinggi pada substrat kulit pisang sebesar 118 x 10-3 nkat/ml. Isolat C4-4 memiliki aktivitas selulase tertinggi pada substrat kulit pisang sebesar 180 x 10-3 nkat/ml. Isolat C5-1 dan C5-3 memiliki aktivitas enzim selulase yang relatif kecil pada substrat CMC, Avicel dan kertas saring sehingga kemampuan enzim selulase isolat tersebut juga relatif kecil dalam mendegradasi substrat limbah pertanian. Namun isolat C5-1 dan C5-3 berturut-turut memiliki aktivitas yang tinggi pada substrat kulit pisang yaitu 262 x 10-3 nkat/ml dan 210 x 10-3 nkat/ml.
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Judul: Karakterisasi Enzim Selulase dan Aplikasinya pada Substrat Limbah Pertanian
Abstrak: Cellulose is the most abundant plant cell walls. Lignocellulolytic are consist of cellulose, hemicelluloses and lignin. Degradation of cellulose involves a complex interplay between different cellulolytic enzymes. Among others, it has been widely accepted that three types of cellulases including endoglucanases, exoglucanases and β-glucosidases act synergistically to convert cellulose to glucose. This study was aimed to characterized cellulase enzyme which produced by cellulolytic bacteria from soil and applied it on agricultural waste such as rice straw, corn cob and banana peel. Qualitative analysis have done on 9 isolates cellulolytic bacteria to know the highest cellulolytic index by using congo red 0.1%. From 9 isolates, 1 isolate have IS 1.75. Quantitative analysis showed isolate 6-2 has the highest cellulase activity of 0.005U/ml. Highest activity of cellulase enzyme from isolate 6-2 resulted on bufer pH 7 and 40oC. Cellulase activity of isolate 6-2 was stable for 75 minutes. Isolate 6-2 has relatively low cellulase activities in CMC as it has small enzyme activity in degrading substrates of agriculture waste. The cellulose enzyme activity on ricestraw resulted 0.007U/ml, corncob resulted 0.008U/ml, and bananapeel resulted 0.013U/ml. Isolate 6-2, gram positive bacteria can degrade cellulose by using cellulase enzyme. On the application cellulase enzyme activity on bananapeel was higher than the others.
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Judul: Implementation of Spatio-Temporal Change Query in Relational Database.
Abstrak: Geographic object changes in various views. Object changes are recorded using a model of static storage. No historical connection between old version data and new version data has been built. For that, a storage model of spatio-temporal queries that can analyze spatio-temporal changes has been developed. The object changes the geometry are grouped according to the taxonomy of Spatio-Temporal Pattern Change. This research has been able to apply the query patterns change including born, expand, merge, contract, split, normal and die. This query based on geometric (area) changes. The implementation of spatio-temporal change queries represents an object changes continuously with easy, change detection is done by comparing two versions of data. Keyword: spatio-temporal change query, spatio-temporal change pattern.
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