Judul: Analisis Biaya Pembuatan Mebel Rotan : Studi Kasus di CV Salsa Rattan, Cirebon, Jawa Barat
Abstrak: Mebel rotan merupakan salah satu komoditas hasil hutan yang berkontribusi terhadap devisa Indonesia. Namun penurunan nilai ekspor dalam beberapa tahun terakhir menunjukkan bahwa telah terjadi penurunan daya saing internasional untuk produk mebel rotan lokal. Studi tentang biaya produksi yang berkaitan dengan mutu produk dan peningkatan efisiensi produksi telah dilakukan di CV Salsa Rotan, Cirebon, Jawa Barat, pada bulan Oktober sampai dengan November 2009.Hasil penelitian yang dilakukan terhadap 5 jenis produk rotan, yaitu kursi Ramona Arm DSB, kursi Ramona Dining DSB, kursi Rollo Dining ANB, kursi Rollo Dining CBC, dan kursi Rollo Dining DSB menunjukkan bahwa : biaya produksi berkisar antara Rp 200.950/unit sampai Rp 222.130/unit; nilai Break Even Point (BEP) bervariasi; dan nilai Return on Investment (ROI) adalah sekitar 25%.
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Judul: Optimasi Produksi Mebel Rotan: Studi Kasus di CV. Dimo Putera Jaya Cirebon Jawa Barat
Abstrak: Industri rotan berkembang sangat pesat terutama setelah pemerintah melalui Surat Keputusan (SK) Menteri Perdagangan No 274/KP/X/86 mengeluarkan kebijakan mengenai:larangan mengekspor rotan mentah dan setengah jadi. Salah satu sentra industri rotan di Indonesia adalah Cirebon. Biro Pusat Statistik (2006) melaporkan pada tahun 2004 di Cirebon industri pengolahan rotan skala besar dan menengah berjumlah 126 perusahaan sedangkan perusahaan pengolahan rotan skala kecil sebanyak 934 perusahaan. Penelitian dilakukan di CV. Dimo Putera Jaya, Cirebon. Perusahaan ini merupakan salah satu perusahaan yang bergerak dalm bidang pembuatan mebel rotan. Dalam menjalankan proses produksinya perusahaan bekerja sama dengan industri rumah tangga pembuat rangka dan anyaman yang ada di daerah sekitar pabrik. Dalam penelitian ini data yang diambil berupa data primer dan data sekunder, yang diperoleh dari hasil wawancara langsung dan studi literatur. Data yang diperoleh kemudian diolah untuk mengetahui besarnya biaya produksi dan keuntungan baik di pengrajin maupun di perusahaan. Sedangkan untuk menghitung optimasi produksi mebel rotan di perusahaan menggunakan program bilangan bulat.
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Judul: DNA Extraction and Characterization of Salmonella‟s Lytic Bacteriophage Genome
Abstrak: Bacteriophages are ubiquitous in nature and are known to proliferate wherever their bacterial hosts exist. Their virion particles can exist independently outside the host, however all phages are need their host to propagate. Several phages are highly specific to host cell surface receptors and any slight changes in structure results in little or no interaction between the phage and its host. Salmonella‟s phage isolated from sewage water where their bacteria exist. Three isolated of phages (phages 15, 19 and 38) were used in this research that concentrated using three types of methods. PEG/ NaCl method was firstly used to concentrate the phages based on their molecular weight to precipitate the phage particles during centrifugation. The second method was Scraping Plaque method. In this method, overlay plaques were scraped and collected in SM buffer to get phage concentrates. Enrichment method was the last method used to concentrate the phages. Phage genome isolation was done using two methods i.e. EDTA/SDS and Proteinase K methods. The first method was done using DNase I to eliminate bacterial DNA followed by SDS/EDTA treatment to release phages DNA and phenol-chloroform-isoamyl alcohol to extract the DNA. DNA was collected by isopropanol extraction. While in Proteinase K method, bacterial DNA was eliminated by DNase I and Proteinase K/SDS was used to degrade phages capsids and ethanol for DNA precipitation. The result showed that PEG/NaCl method resulted the highest phage concentration for phage 15 and phage 19, while scrapping method was the highest for phage 38. The quantity and purity of extracted DNA were low for PEG/NaCl and Scraping methods, while Enrichment method was higher indicated higher purity. Run on agarose gel electrophoresis of extracted DNA confirmed that Enrichment method results were the best while scraping and PEG/NaCl method was appeared smear background. Based on DNA genome size determination, the bacteriphages of Salmonella sp were comparable with other study of Salmonella phages. Phage P15 with genome size of 39,5 kb was close to phage epsilon15. Phage P19 with genome size of 41 kb was close to phage ST160. And phage P38 with genome size of 48,5 kb was close to phage Gifsy-1.
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