Judul: Efek Genistein terhadap Derajat Alergi dan Kadar Imunoglobulin E Marmut yang Diinduksi Putih Telur
Abstrak: Research about the effect of genistein on immunoglobulin E level has been done by using guinea pig as the murine model. Guinea pigs were divided into two groups namely control and genistein. Control group were not sensitisized, while genistein were sensitisized for 40 days by sub cutaneous injection of egg white, NaCl 0.9%, and Al(OH)3 for 30 days, continued by induced egg white in drinking water between day 31 until day 39. On day 40th all these guinea pig were injected with serum in dorsal surface. Day 42nd control group were induced orally with 1 mL aquadest, while genistein group induced orally with 1.84 μg/100 g guinea pig’s body weight in 1 mL aquadest. All guinea pigs then induced with egg white as the allergen and Evan’s Blue dye intravenously. Blood sample were taken before sensitization and after induction of egg white. Immunoglobulin E level of blood sample was calculated by ELISA method, allergic degree were measured by Pasive Cutaneous Anaphylaxis (PCA). PCA analysis shown that control group and genistein group has the equal bluish intensity. IgE level also support the PCA data, thus, it can not be stated that genistein induction alter the decrease of immunoglobulin E level.
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Judul: Pemberian Ovalbumin Sebagai Penyebab Alergi pada Marmot
Abstrak: Most people consider allergic is not dangerous. However in some cases may cause death. Allergic has been suspected to relate with immunoglobulin E (IgE) formation, followed by the release of histamine and other cells mediator, until the symptom occur. In this research, the effect of ovalbumin induction on allergic reaction was studied. The used guinea pigs were divided into control (K) and ovalbumin treatment groups, each containing three guinea pigs. Animals were adapted for 14 days then 40 days albumin induced for allergic reaction. Guinea pig’s blood were taken after 40 days sensitization and injected back (anafilaksis cutaneous pasif) to the animals. Evans blue were given after 48 hours serum injection, to obtain bluish spot formation for symptom observation. Research showed that ovalbumin induced allergic reaction. Anafilaksis cutaneous passive testing showed that the bluish diameter didn’t occure on guinea pig’s back, but the allergic effect occurred tend to systemic effect.
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Judul: Cycle Hamilton pada Graf Lengkap, Graf Regular, dan Graf 2-Connected 4-Regular Berorder Kurang dari Sepuluh
Abstrak: Suatu graf disebut graf Hamilton apabila graf tersebut memuat cycle Hamilton, yaitu cycle yang melewati setiap vertex tepat satu kali. Menentukan keberadaan cycle Hamilton pada suatu graf dapat menggunakan syarat cukup yang berupa teorema-teorema yang telah diperkenalkan sejak tahun 1950-an. Beberapa syarat cukup yang dibahas dalam karya ilmiah ini adalah syarat cukup yang berhubungan dengan graf lengkap dan graf regular. Suatu graf lengkap berorder 𝑛������ ≥ 3 dan graf r-regular berorder 𝑛������ ≤ 2𝑟������ + 1 merupakan graf Hamilton, Karena kedua graf tersebut memuat cycle Hamilton. Selanjutnya, syarat cukup tersebut digunakan untuk menentukan keberadaan cycle Hamilton pada graf 2-connected 4- regular berorder kurang dari 10.
Keyword: graf Hamilton, graf regular, graf lengkap, graf 2-connected 4-regular