Judul: Dinamika perubahan penggunaan lahan dan strategi pengalokasian ruang terbuka hijau berdasarkan penganggaran daerah berbasis lingkungan (studi kasus Kota Bekasi)
Abstrak: Marginalization issue of Green Open Space (GOS) with a high level of land conversion from vegetative or agriculture land to built land in urban area shows that there is no commitment of regional government on a sustainable urban development. Political commitment on the regional government is indicated among others by the weak support of green regional budgeting (APBD) related to GOS. The research was conducted in Bekasi City. The purpose of this research was to determine a model of environmentally sound regional budgeting policy strategy related for allocation of public GOS by using a hard systems and a soft systems approaches. The former was conducted by landuse changes analysis with the factors influencing them, by designing a regional budgeting based on GOS allocating model structure by using a dynamic system approach by formulating the direction policy using focus group discussion (FGD) and analytical hierarchy process (AHP). The results of landuse change analysis showed that there was an increase in built land area from 5.5% (1,157.77 ha) in 1989 to 70.7% (14,879.85 ha) in 2009. The determinants of landuse changes in GOS were population, educational facilities, markets, supermarkets, settlements, industries, restaurants, hotels, and inns (R2 = 99.6%). The dynamic model was designed three scenarios of GOS allocating policy strategy (pessimism, moderate, and optimism) with an early simulation in 2010. The optimism scenario was considered as being capable of accommodating the fulfillment of city GOS need really on an assumption of considerable long multiyears budgeting so that in 2030 the target of 20% public GOS would be achieved. The results of analysis by AHP and FGD approaches showed that alternatives were on 2 main policies, namely: agriculture/GOS infrastructure development and GOS land acquirement. The priorities of policies emphasized the importance of commitment to green budgeting with tight control, particularly tax disincentive and tightening of building license.
Keyword: green budgeting, bekasi, jawa barat, ruang terbuka hijau

Judul: Analisis perubahan ruang terbuka hijau dan strategi pengembangannya di Kota Bandar Lampung:
Abstrak: Development of Bandar Lampung City has the great influenced to the land use changes. Land use conversion commonly happen on Green Open Space (GOS) into other usages. The aims of this research are : (a) to identify GOS change in Bandar Lampung City along the year 2000-2007; (b) to identify GOS needed base on regulation of spatial planning (UU No 26/2007) and number of population; (c) to identify center of GOS change into built up area related to the regional development and the factors that affected the change; (d) to identify divergency of GOS change in RTRW (Public Document of Regional Planning); and (5) to find the strategy to maintain and develop GOS. This research was conducted by Landsat analysis using Geographic Information System (GIS) and combined with Location Quotient Analisys (LQ) to analize GS change. Scalogram and regression analysis used for analize the regional development and the factors that affected the GOS change. The result showed that forest, mixed dry farming, plantation and paddy field decrease of 1.449 Ha (7.35%) in 7 years, since year 2000 to 2007. Meanwhile, built up area increase to 1.218 Ha (6,19%). Based on UU No 26/2007, availability of GOS in Bandar Lampung City are still sufficient (> 30%), it’s about 61.40% (12.110 Ha) from the total area of the city. Analysis of GOS needed based on area found that in subdistrict level at Tanjung Karang Pusat and Kedaton subdistrict insufficient GOS. Based on standard for number of population, Tanjung Karang Pusat, Kedaton and Teluk Betung Selatan subdistrict still in below standard. The changes center of GOS in Bandar Lampung City happen in 63 villages, which are distributed in hierarchy III 79,57 % villages, hierarchy II 16,60% village, and hierarchy I 3,83% village. There are 2 factors that affected GOS change, one is village development index and another is number of population. Land use change from GOS and protection area into built up area was found as deviation of RTRW. The strategy to maintain and to develop GOS depend on the availability and the problem that occur with GOS, so GOS can be developed optimally.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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