Judul: Analisis dijital tekstur citra sar (Syinthetic aperture radar) sir-B (Shutle imaging radar-B)  : Studi kasus daerah sebakung, Kalimantan Timur
Abstrak: Tekstur merupakan frekuensi perubahan rona pada suatu citra dan sering dinyatakan dengan kasar, sedang, dan halus. Akhir-akhir ini tekstur mulai digunakan sebagai unsur interpretasi yang digunakan dalam dan analisis citra. interpretasi Perubahan tekstur penting bagi tujuan analisis citra, karena perubahannya merupakan indikasi terjadinya perubahan suatu daerah atau objek pada suatu citra. Analisis citra melalui pendekatan tekstural akan memberikan informasi mengenai distribusi spasial dan susunan gaman nilai dijital. kera- Suatu metode analisis tekstur yang tergolong baru yaitu metode SDTT (Similarly Dependence Textural Trans- formation) digunakan untuk transformasi citra melalui pendekatan statistik. Metode ini didasarkan pada penggunaan gabungan antara penguatan (enhancement) dari ting- kat keabuan citra (grey level) oleh kookurensi, serta penghitungan rata-rata oleh suatu operator filter lokal. Perubahan-perubahan dalam tekstur dapat dideteksi dengan memilih parameter-parameter masukan yang sesuai yang sangat berkaitan dengan pola tekstur yang akan dianalisis. Pengoperasian metode ini sederhana, berdayaguna dan tidak tergantung dari orientasi angular pola masukan. Citra yang digunakan dalam penelitian ini merupakan citra SAR SIR-B daerah Kalimantan Timur yang direkam oleh pesawat ulang alik Challanger pada tahun 1984. ...
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Judul: Identifikasi Pola Penutup Lahan Melalui Analisis Dijital Citra Sar Strat-1 Studi Kasus Daerah Gemar Baru, Muara Ancalong Kalimantan Timur
Abstrak: Pengenalan obyek pada suatu citra radar dapat dilakukan dengan interpretasi melalui analisis visual maupun analisis dijital. Analisis dijital dapat dilakukan dengan klasifikasi tidak terbimbing (unsupervised classification) atau dengan analisis terbimbing (supervised classification). Tujuan penelitian ini adalah untuk mempelajari perbandingan teknik klasifikasi secara dijital untuk keperluan identifikasi penutup lahan hutan dari citra SAR STAR-1 yang telah mengalami transformasi tekstur. Penelitian ini dilaksanakan di Laboratorium Penginderaan Jauh dan Kartografi Jurusan Tanah mulai bulan Januari sampai dengan Agustus 1992. Citra radar yang digunakan adalah SAR STAR-1 daerah Gemar Baru, Huara Ancalong Kalimantan Timur. Sebelum dilakukan interpretasi secara tidak terbimbing maupun secara terbimbing, citra asli diberi berbagai perlakuan untuk mengatasi gangguan-gangguan yang dapat menyebabkan berkurangnya informasi yang dikandung dalam suatu citra radar. Filter median dan filter rata-rata (LPF) digunakan sebagai metode untuk mengurangi derau spekel yang ditemui pada citra.
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Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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