Judul: Deteksi Gen hpt dan bar untuk Uji Kestabilan Transposon Ac/Ds pada Padi Mutan IR64
Abstrak: Ac/Ds transposon system can be used to gain variety of mutant rice genotype and for identifying the function of mutant gen especially IR64 rice. However to obtain mutant plant that we want, stable transposon is required in order to avoid transposon movement to other chromosoms. This research has analyzed 26 samples of genom DNA of first generation (T0) plants with polymerase chain reaction (PCR) using bar and hpt primer also Southern hybridization analyzing. There is 26 genom DNA samples of second generation (T1) plants analyzed with PCR. PCR analyzation showed that transposon Ac/Ds is occur in all samples. Southern hybridization showed that 14 of 26 T0 plants contain single copy of T-DNA. Fourteen T1 generation plants contain stable Ds transposon based on the results of PCR analysis. This research proved that Ds transposon is stable because it was inherited on the next generation
Keyword: stability analysis, Ac/Ds transposon, IR64

Judul: Analisis Molekuler dan Fenotipe Galur Padi Transgenik IR64 Generasi T3 yang Mengandung Penanda Aktivasi untuk Toleransi Kekeringan
Abstrak: Activation tagging transgenic rice cv. IR64 had been developed by introducing a construct of activation tagging transposon Ac/Ds. Phenotipic character associated with drought stress tolerance of the transgenic rice lines have not been analyzed. This study aimed to evaluate T3 generation of activation tagging transgenic rice lines cv. IR64 to drought stress treatment. The methodology of study consisted of several steps including germination of samples seeds, screening the seedling with drought strees treatment, molecular analysis of the rice lines identify hpt and bar genes, and observation of agronomic character. Results of study showed that there were 34 out of 84 plants that tolerant to drought stress. A total of 23 plants of tolerant plants were detected carrying only bar gene after PCR analysis and indicated that the plants were stable mutants. Generaly, compared to the non-transgenic plants, there were changes in several agronomic parameters of transgenic rice plants cv. IR64 T3 generation. A total of nine plants increased of its height, six plants had a faster harvesting time, and 21 plants had higher weight of 100 seeds compared by non-transgenic plants.
Keyword: Bogor Agricultural University (IPB), drought stress tolerance, activation tagged, Rice (Oryza sativa L.)

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
Keyword: