Judul: Komposisi Struktur dan Penyebaran Bidara (Ziziphus mauritiana Lamk.) pada Savana Bekol Taman Nasional Baluran
Abstrak: Savana merupakan ekosistem khas yang berada di Taman Nasional Baluran (TNB). Jenis bekol atau bidara (Ziziphus mauritiana Lamk.) menjadi jenis endemik savana, khususnya Savana Bekol. Permasalahan muncul ketika tanaman eksotik seperti Acacia nilotica masuk sebagai tanaman sekat bakar alami. Pertumbuhan A. nilotica tidak terkendali sehingga merubah kondisi alami savana. Tujuan dari penelitian adalah mengetahui komposisi struktur dan penyebaran Z. mauritiana pada Savana Bekol baik yang terpelihara dan yang sudah terinvasi A. nilotica. Penelitian dilakukan melalui analisis vegetasi dengan plot berpetak yang diletakan secara purposive dengan ukuran 20 m x 60 m sebanyak 10 jalur pada setiap lokasi. Hasil penelitian menunjukan bahwa komposisi struktur Savana Bekol terpelihara masih menjadi daerah terbuka dengan kerapatan pohon yang jarang dan rumput yang melimpah sedangkan daerah terinvasi berubah menjadi hutan A. nilotica yang tertutup. Kerapatan Z. mauritiana di Savana Bekol terpelihara lebih banyak sebesar 3 364 ind/ha dibandingkan di Savana Bekol terinvasi hanya 260 ind/ha Penyebaran Z. mauritiana pada Savana Bekol adalah mengelompok. Faktor lingkungan yang paling mendukung terkait penyebaran Z mauritiana adalah daerah yang terbuka, selain terkstur tanah liat, pH yang alkalin dan iklim yang kering.
Keyword: komposisi struktur, penyebaran, bidara (Z. mauritiana), dan Savana Bekol TNB

Judul: Understory Vegetation Diversity and Forage  Plants Availability in Bekol and Balanan Savanna, Baluran National Park
Abstrak: Akasia berduri (Vachellia nilotica) adalah salah satu tumbuhan yang berada di Taman Nasional Baluran yang bersifat invasif dan memengaruhi komposisi savana di kawasan tersebut. Penelitian bertujuan mengetahui keanekaragaman spesies tumbuhan bawah, sifat invasif dan mengkaji spesies pakan satwa yang mampu berkompetisi pada lingkungan penelitian. Pengambilan data analisis vegetasi didapatkan dengan metode kuadrat. Plot utama dengan ukuran 50 m × 50 m dibuat pada tiga kawasan, yaitu kawasan savana terinvasi rendah, tinggi, dan bebas invasi. Setiap plot utama dibuat 15 petak contoh yang berukuran 1 m × 1 m. Parameter tumbuhan bawah yang diamati adalah jumlah individu dan spesies. Pada savana terinvasi rendah ditemukan sembilan spesies dari tujuh famili dan pada savana terinvasi tinggi ditemukan sembilan spesies dari enam famili. Nilai indeks keragaman jenis (H’) pada savana Balanan invasi rendah bernilai 1,62 dan pada invasi tinggi sebesar 1,74, sedangkan pada savana Bekol yang terbebas invasi sebesar 1,24. Vegetasi yang menyusun ketiga kawasan cenderung berbeda dengan nilai indeks kesamaan Sorenson (IS) sebesar 4.05 hingga 14.65. Jumlah spesies pakan pada kawasan invasi tinggi lebih rendah dibandingkan dengan kawasan lainnya., Thorny acacia (Vachella nilotica) is one of the invasive plants species in Baluran National Park (BNP) which affects the vegetation composition in its savanna. This study aims to determine the diversity of understory species and its invasiveness, as well as to examine the availability of forage plant species that are able to compete with the other understory species in the research plot. This study was conducted in three areas that is high invaded, low invaded, and uninvaded areas from V. nilotica. The vegetation composition was described by vegetation analysis. Main plot with a size of 50 m × 50 m has been made in three areas with five replicate plots were selected for each location resulting in total of 15 plots were studied. The uninvaded area has the highest number of understory species compare against the high invaded and low invaded areas. The value of the species diversity index (H') in high invaded, low invaded, and uninvaded areas are 1,74; 1,62; and 1,24 respectively. The understory composition within the three areas were tends to be different based on Sorenson Similarity Index (IS) with a range of values 4.05 to 14.65. The high invasion area has the lowest number of forage plants species compared to other areas.
Keyword: animal feed, invasion, quadrat method, Vachellia nilotica, vegetation analysis

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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