Judul: Tingkat Stres Dan Strategi Koping Ibu Pada Keluarga Dengan Anak Retardasi Mental
Abstrak: Tujuan umum penelitian ini adalah untuk mengetahui tingkat stres dan strategi koping ibu pada keluarga dengan anak retardasi mental. Secara kbusus penelitian ini bertujuan untuk mengetabui : 1) karakteristik ke[uarga, karakteristik anak, dan karakteristik ibu, 2) dukungan keluarga, kondisi psikososial anak, dan persepsi ibu, 3) hubungan antara karakterlstik keluarga dengan dukungan keluarga, karakteristik anak dengan kondisi psikososial anak, dan karakteristik ibu dengan persepsi ibu, 4) tingkat stres ibu, 5) hubungan antara dukungan keluarga, kondisi psikososial anak, dan persepsi ibu dengan tingkat stres ibn, 6) strategi koping ibu, dan 7) hubungan antara tingkat stres dengan strategi koping stres ibu.
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Judul: Strategi Koping, Tingkat Stres Ibu Rumah Tangga dan pengaruhnya Terhadap Komunikasi Keluarga
Abstrak: Komunikasi keluarga merupakan hal penting dalam menciptakan keakraban, keterbukaan, perhatian antaranggota keluarga terlebih pada masa krisis pandemi Covid-19 ini. Penelitian ini bertujuan untuk mengetahui strategi koping dan tingkat stres terhadap komunikasi keluarga. Penelitian ini melibatkan 50 keluarga berdomisili pedesaan dan 50 berdomisili perkotaan yang memiliki anak usia remaja (minimal usia sekolah menengah pertama) di seluruh Indonesia. Pengambilan contoh penelitian dilakukan secara non probability sampling dengan menggunakan teknik voluntary sampling. Hasil uji korelasi menunjukkan bahwa terdapat hubungan signifikan positif pada strategi koping dan hubungan negatif pada tingkat stres pada komunikasi keluarga. Hasil uji regresi menunjukkan bahwa strategi koping berpengaruh positif signifikan dan tingkat stres berpengaruh negatif signifikan terhadap komunikasi orientasi percakapan. Selain itu, hasil uji regresi menyatakan bahwa strategi koping berpengaruh positif signifikan dan tingkat stres berpengaruh negatif signifikan terhadap komunikasi orientasi konformitas., Family communication is one of the things to create solidarity and attention for family members. Exceedingly during this Covid-19 pandemic. This study aims to determine coping strategies and stress levels on family communication. This study involved 50 families living in rural areas and 50 living in urban areas with teenage children (minimum junior high school age) throughout Indonesia. A sampling of the study by nonprobability sampling using the voluntary sampling technique. Results of correlation test showed coping strategies have a positive significance and stress levels have negative significance in family communication. Results of regression test showed that coping strategy has a significant positive effect and stress levels have a negative significant effect on family communication conversation orientation. In addition, the results of the regression test stated that the coping strategy has a significant positive effect and stress levels have a significant negative effect on family communication conformity orientation.
Keyword: coping strategy, family communication, family with teenagers, housewife, stress level

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.