Judul: Pengembangan Metode Isolasi Total RNA, mRNA dan Sintesis cDNA Sengon (Falcataria moluccana L. Nielsen) yang Sehat dan Terserang Hama Boktor (Xystrocera Festiva).
Abstrak: Sengon (Falcataria moluccana L. Nielsen) has a natural self-defense mechanism towards pest infection by synthesizing certain macromolecul such as tripsin inhibitor and alfa-amilase inhibitor. Characterization of those genes which produce the inhibitor need to be done on resistant trees and the vulnerable ones, to define the differences so that can be used for tree improvement. This research aim to develop an appropriate isolation methods of total RNA, mRNA and cDNA of plants that have a inhibitor enzymes such as sengon. The materials used in this research were cambium and timber tissue from resistant and vulnerable tree. Total RNA was isolated using Total RNA Mini Kit by Geneaid, mRNA was isolated using PolyATract mRNA Isolation System III with Magnetic Stand by Promega and cDNA synthesize was conducted using cDNA Substraction Kit by Clontech and RevertAid RT Transcription Kit by Thermo. The highest concentration of total RNA isolation were 65,60ng/μl from modification methods, and the best mRNA yields was resulted by modification methods. The highest concentration for cDNA synthesize were 2296,17ng/μl from modification methods.
Keyword: sengon, RNA and mRNA isolation, cDNA synthesize

Judul: α-Amylase Inhibitor Gene Expression of Healthy Sengon (Falcataria moluccana Miq) Healthy and Attacked by Boktor Pests
Abstrak: Sengon (Falcataria moluccana Miq) merupakan salah satu pohon pionir dan memiliki berbagai macam kegunaan. Penanaman sengon secara monokultur menimbulkan terjadinya ledakan hama penggerek batang atau boktor (Xystrocera festiva) yang merusak kayu dan menjatuhkan nilai jual batang sengon. Studi molekuler aktivitas gen α-amilase inhibitor (AAI), yang merupakan salah satu gen ketahanan terhadap hama boktor sangat mendukung program pemuliaan sengon. Penelitian ini bertujuan mengkuantifikasi tingkat ekspresi gen α-amilase inhibitor sengon yang sehat dan terserang hama boktor. Prosedur penelitian ini meliputi isolasi RNA total, sintesis cDNA, perancangan primer spesifik gen AAI (target) dan aktin (kontrol), optimasi suhu annealing primer gen AAI dan aktin, ekspresi gen AAI dan aktin pada Real Time PCR dan analisis data. Fragmen cDNA teramplifikasi pada suhu annealing 61,7oC untuk gen aktin dan 61oC untuk gen AAI. Tingkat ekspresi gen AAI 2 pada pohon sengon yang terserang hama boktor memiliki nilai yang lebih tinggi yaitu 55,60 pada sampel 1 dan 6,96 pada sampel 3.
Keyword: Falcataria moluccana, α-Amilase Inhibitor, Real Time PCR

Judul: Metrik Einstein (Anti-) Self-Dual Berdimensi Empat dengan Metode Cartan
Abstrak: Gravitational field equation can be formulated in differential forms (differential geometry), which describes gravity as a manifestation of the curvature of space-time. Curvature of space-time is characterized by a metric that satisfies the gravitational field equation. With differential forms it can be formulated the (anti-) self-dual Einstein equation, which is a modification of the Cartan structure in (anti-) self-dual basis. Calculation of (anti-) self-dual metric with (anti-) self-dual Einstein equation is simpler than the calculation using Christoffel symbols.
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