Judul: Analisis kebijakan pengelolaan taman hutan raya (Studi kasus di TAHURA Sesaot Propinsi Nusa Tenggara Barat)
Abstrak: Penelitian ini bertujuan (a) untuk mengetahui faktor-faktor penyebab terjadinya kerusakan kawasan Taman Hutan Raya Sesaot (b) menganalisis alternatif pemanfaatan yang optimal kawasan Taman Hutan Raya Sesaot.
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Judul: Analisis Strategi Kebijakan Pengelolaan Taman Hutan Raya Berbasis Ekososiosistem (Studi Kasus Tahura Wan Abdul Rachman Di Propinsi Lampung)
Abstrak: The objective of this research is to determine the characteristics of the problems in WAR Grand Forest Park, to determine a comprehensive management based on ecology, economy and socioculture and to develop a zonation suitability map in relation to sustainable management of WAR Grand Forest Park. The method used are descriptive, qualitative and quantitative. Analysis of the characteristic of the problem of WAR Grand Forest Park was analyzed by using tabulation and descriptive methodes. Policy strategic analysis for WAR Grand Forest Park management was analyzed using PHA method and SWOT. GIS was used as direction to determine the optional zonation for WAR Grand Forest Park based on strategic policy analysis. The result showed that degradation of WAR Grand Forest Park is caused by: 1) land resources availability (Open access), 2) indiscipline staff in WAR Grand Forest Park, 3) lack of law enforcement, 4) social economic and welfare motivation. PHA analysis showed that the main strategy for management of Tahura WAR is ecotourism (value: 0.402 or 40.2%) followed by ecotourism-agro forestry (value 0.386 or 38.6%), and Agro forestry (value 0.212 or 21.2%). Operational strategies that must be carryout by government are: (1) reducing illegal logging, (2) Policy regulation, (3) law enforcement for indiscipline staffs, (4) removal of regulation that contrast to the local government regulation such as Perda No. 7/2000 on retribution, (5) produce zonation suitability map for ecotourism purpose, (6) develop collaboration management of ecotourism in WAR grand forest park with the communities around Tahura WAR. Spasial analysis showed that region allocated for ecotourism purpose in WAR grand forest park has a topography of 600 mdpl and countur less than 40%.
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Judul: Influenza Specific Immunoglobulin Y Purification from Egg Yolk with Sodium Chloride Precipitation Method
Abstrak: Imunoglobulin Yolk (IgY) yang terdapat dalam kuning telur telah banyak dimanfaatkan untuk imunoterapi dan imunodiagnostik. Salah satu kendala dalam pemurnian IgY dari kuning telur adalah pemisahan Ig dengan lemak, sehingga teknik pemurnian Ig selalu berkembang. Penelitian ini bertujuan untuk melakukan pemurnian IgY dari kuning telur dengan metode pengendapan menggunakan sodium chloride (NaCl) serta melakukan karakterisasi IgY spesifik influenza. Sodium chloride dipilih karena bahan kimia ini relatif murah, ramah lingkungan, dan tidak merusak imunoaktivitas IgY. Kuning telur yang digunakan dalam penelitian ini berasal dari telur ayam layer yang divaksinasi dengan vaksin influenza Flubio® yang berisi virus Influenza A/H3N2, A/H1N1 dan B/Washington. Pemurnian IgY hanya dilakukan pada kuning telur yang mengandung antibodi spesifik influenza. Deteksi keberadaan antibodi spesifik influenza ditentukan dengan uji agar gel presipitasi (AGP) dan metode enzyme linked immunosorbent assay (ELISA). Konsentrasi IgY hasil pemurnian dihitung dengan spektrofotometer dan karakterisasi protein dilakukan dengan metode Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Konsentrasi protein dari hasil pemurnian IgY memiliki nilai rata – rata 50,403 mg/mL. Hasil SDS-PAGE menunjukkan enam pita protein, yaitu struktur IgY utuh (174,63 kDa), Apoprotein-LDL (129,77 kDa), rantai berat IgY (66,54 kDa), Fab ( 47,65 kDa), rantai ringan IgY (21,85 kDa), dan β-livetin (36,74 kDa). IgY hasil pemurnian masih dapat berikatan dengan antigen influenza berdasarkan uji ELISA. Hasil penelitian ini menunjukkan bahwa pemurnian IgY menggunakan metode pengendapan NaCl dapat memurnikan IgY dengan struktur utuh, meskipun masih ada dua protein tidak spesifik hasil pemurnian. IgY yang dapat berikatan dengan antigen influenza mengindikasikan prospek pemanfaatannya untuk pencegahan influenza dan imunodiagnostik., Yolk immunoglobulin (IgY) contained in egg yolk has been widely used for immunotherapy and immunodiagnostics. One of the obstacles in IgY purification from egg yolk is separating Ig from fat, so that IgY purification techniques are always developing. This study aimed to purify IgY from egg yolk using sodium chloride (NaCl) precipitation method and to characterize influenza-specific IgY. Sodium chloride was chosen because this chemical is relatively unexpensive, environmentally friendly, and does not impair IgY immunoactivity. The egg yolks used in this study were collected from layer eggs vaccinated with the Flubio® influenza vaccine, which contains the Influenza A/H3N2, A/H1N1 and B/Washington viruses. IgY purification was only carried out on egg yolks containing influenza specific antibodies. The presence of influenza-specific antibodies was determined by the agar gel precipitation (AGP) test and the enzyme linked immunosorbent assay (ELISA) method. The purified IgY concentration was determined using spectrophotometer, and protein characterization was performed using the Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) method. Protein concentration from purification has a mean value of 50,403 mg/mL . SDS-PAGE result showed six protein bands, namely intact IgY structure (174,63 kDa), Apoprotein-LDL (129,77 kDa), IgY heavy chain (66,54 kDa), Fab (47,65 kDa), IgY light chain (21,85 kDa), and β-livetin (36,74 kDa). The purified IgY still able to bind with influenza antigen based on the ELISA test. The results of this study indicate that purification of IgY using the NaCl precipitation method can purify IgY with intact structure, although there are still two unspecific proteins in the purification results. The resulting IgY can bind to influenza antigens indicating the
Keyword: Immunoglobulin Y, influenza, egg yolk, purification, sodium chloride