Judul: Application of EPA SWMM 5.2 on Drainage Channel Evaluation in Harapan Indah Complex Medan Satria District Bekasi City
Abstrak: Kasus banjir berulang setiap musim hujan di Indonesia. Kota Bekasi, khususnya Komplek Harapan Indah RW 20 hampir setiap tahun mengalami banjir dengan genangan setinggi 60 cm menjadi wilayah prioritas penanganan banjir. Penting untuk menilai kapasitas saluran drainase dan kesesuaiannya dengan volume limpasan air menggunakan model EPA SWMM 5.2 yang efektif menganalisis masalah limpasan di perkotaan. Penelitian ini bertujuan mengevaluasi kesesuaian saluran drainase dan memperbaiki saluran yang meluap. Penelitian ini terdapat luas wilayah ± 6,81 ha, menggunakan data dimensi saluran, curah hujan harian maksimum, dan peta kontur. Metode distribusi yang sesuai yaitu Log Pearson III dengan periode ulang 5 tahun, menghasilkan intensitas curah hujan rencana 267,88 mm. Pemodelan mencakup 20 subcatchment (SUB), 70 conduit (con), 48 junction (j), dan 2 outfall (Out). Puncak hujan terjadi pada jam ke-6 dengan intensitas sebesar 25,93 mm. Debit limpasan tertinggi pada Sub5 dengan nilai 0,07 m3/det. Terdapat luapan pada saluran drainase yaitu Con23, Con34, Con37, Con40, Con43, dan Con46 dikarenakan terdapat sedimentasi. Setelah dilakukan perbaikan menunjukan saluran yang meluap kini mampu menampung debit air yang masuk.
Keyword: banjir, curah hujan, drainase, EPA SWMM

Judul: Application of EPA SWMM 5.2 on Drainage  Channel Evaluation in Harapan Indah Complex Medan Satria District Bekasi City
Abstrak: Kasus banjir berulang setiap musim hujan di Indonesia. Kota Bekasi, khususnya Komplek Harapan Indah RW 20 hampir setiap tahun mengalami banjir dengan genangan setinggi 60 cm menjadi wilayah prioritas penanganan banjir. Penting untuk menilai kapasitas saluran drainase dan kesesuaiannya dengan volume limpasan air menggunakan model EPA SWMM 5.2 yang efektif menganalisis masalah limpasan di perkotaan. Penelitian ini bertujuan mengevaluasi kesesuaian saluran drainase dan memperbaiki saluran yang meluap. Penelitian ini terdapat luas wilayah ± 6,81 ha, menggunakan data dimensi saluran, curah hujan harian maksimum, dan peta kontur. Metode distribusi yang sesuai yaitu Log Pearson III dengan periode ulang 5 tahun, menghasilkan intensitas curah hujan rencana 267,88 mm. Pemodelan mencakup 20 subcatchment (SUB), 70 conduit (con), 48 junction (j), dan 2 outfall (Out). Puncak hujan terjadi pada jam ke-6 dengan intensitas sebesar 25,93 mm. Debit limpasan tertinggi pada Sub5 dengan nilai 0,07 m3/det. Terdapat luapan pada saluran drainase yaitu Con23, Con34, Con37, Con40, Con43, dan Con46 dikarenakan terdapat sedimentasi. Setelah dilakukan perbaikan menunjukan saluran yang meluap kini mampu menampung debit air yang masuk., The problem of flooding recurs every rainy season in Indonesia. Bekasi City, especially the Harapan Indah RW 20 Complex, experiences flooding almost every year with inundation as high as 60 cm, making it a priority area for flood management. It is important to assess the capacity of drainage channels and their suitability to the volume of water runoff using the EPA SWMM 5.2 model which effectively analyzes runoff problems in urban areas. This study aims to evaluate the suitability of drainage channels and repair overflowing channels. This study has an area of ± 6.81 ha, using data on channel dimensions, maximum daily rainfall, and contour maps. The appropriate distribution method is Log Pearson III with a return period of 5 years, resulting in a planned rainfall intensity of 267,88 mm. Network modeling includes 20 subcatchments (SUB), 70 conduits (con), 48 junctions (j), and 2 outfalls (Out). The peak rainfall occurred at the 6th hour with an intensity of 25,93 mm. The highest runoff discharge is in Sub5 with a value of 0.07 m3/det. There are overflows in the drainage channels, namely Con23, Con34, Con37, Con40, Con43, and Con46 due to sedimentation. After the improvement, it shows that the overflowing channel is now able to accommodate the incoming water discharge.
Keyword: drainage, flood, rainfall, EPA SWMM 5.2, GPM satellite

Judul: Isolasi dan Karakterisasi Promoter Gen AGAMOUS (TcAG) dari Kakao (Theobroma cacao L.)
Abstrak: Cocoa is one of the plantation commodities that is important to the national economy. Various efforts have been made to increase cocoa production. One such efforts in the field of genetic engineering discovery for the promoter of gene that has important role in flowering and fruiting. The aim of this research to isolate the promoter of reproductive gene AGAMOUS from Theobroma cacao L. (TcAG) using Thermal Asymmetric Interlaced Polymerase Chain Reaction (TAIL PCR) method and obtain the characteristics of the promoter using a computer program online Softberry. Technique of TAIL PCR using the reverse genespecific primers TcAG position +202 (TcAG+202) (5'- GGCCACGGCTAGAGAAGACAA-3') and position +67 (TcAG+67) (5’- ATCTCGATCTTTCCCCTTCCC-3'). Forward primer AP140 provided the best results are fragment genomic size of 1200 bp. Analysis of Transcription Start Site Prediction (TSSP) of softberry program from the 5' of the fragment has proven that it has three promoter regions. The regions which are nucleotide in position 126 (TATA box at position 93), nucleotide at position 735 (TATA box at position 702) and nucleotide at position 807 (TATA box at position 774). TATA box at position 774 is predicted to be the functional binding sites of RNA polymerase because it is in the position closest to the transcription start site.
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