Judul: Lumut Terestrial dan Ganggang Asosiasinya di Kebun Raya Bogor
Abstrak: Bogor Botanical Garden is an ex-situ conservation which may serve as habitat for bryophyte. Terrestrial bryophyte usually grow on various substrates in shaded habitat or open habitat, such as roadside, along ditches, and rivers. One of the bryophytes benefit in ecosystem is to provide microhabitat for algae. This research was carried out to inventory the bryophyte species grow on terrestrial substrate, and to study their diversity and algal association. The bryophyte were collected on various terrestrial substrates (soil, root, and rock). Quadrates of 25 × 25 cm² were used to sample bryophytes along 30 m transect on three blocks which is dominated by plants with different family, i.e. Meliaceae, Myrtaceae, and Lauraceae to study the diversity. Bryophyte were also sampled used sub-quadrates of 2,5 × 2,5 cm² in the quadrate of 25 × 25 cm² to sample algae associated with bryophyte. Forty three species of terrestrial bryophytes were identified, including 33 species of mosses and 10 species of liverworts. The bryophytes diversity was significantly different when compared to different blocks (p < 0,05). The highest diversity of bryophyte was on block Meliaceae which were more intensively shaded than on any other block. The bryophytes composition was different inter block. Fissidens crassinervis var. laxus, Isopterygium bancanum, and Taxiphyllum cf. taxirameum were dominated on three blocks in Bogor Botanical Garden. These three bryophytes species are commonly grow on soil and roots. Thirteen morphospecies of algae were found in association with terrestrial bryophyte, i.e. Cyanophyta, Chrysophyta, and Chlorophyta.
Keyword: 

Judul: Keanekaragaman Lumut Epifit pada Gymnospermae di Kebun Raya Bogor
Abstrak: Researchs on diversity of epiphytic bryophytes are more common observed on Angiospermae than those on Gymnospermae. Bogor Botanical Garden (BBG) is an ex-situ conservation in which the sample plants are collected around the world for conservation purpose. Trees in the Botanical Garden provide substrats for epiphytic bryophytes. The temperature and humidity in the Botanical Gardens were the optimum range for the bryophyte growth. The aim of this research was to observe diversity of epiphytic bryophytes on Gymnospermae in BBG. The bryophyte samples were collected from Gymnosperm trees in Blok I which is Cycadaceae familly and those in Blok II which are the familly of Pinaceae, Araucariaceae, Gnetaceae, dan Podocarpaceae. Only the trees have ≥ 20 cm (dbh) of trunk base were sampled. Bryophytes were collected from the trunk base at 0-200 cm above the ground, at four cardinal direction (north, south, west, and east). In total 18 species of epiphytic bryophytes were found, included 12 genera and 7 families. The family which had the highest number of species was Lejeuneaceae. The most common species on Gymnosperm trees in the Botanical Garden were Lejeunea tuberculosa and Octoblepharum albidum. Both of them were found at 6 trees of 13 total tree samples. The species were found more frequently at west (2,1%) and north (1,8%) part than east and south part of the host trees. They more frequently inhabit the trunk base on 0-100 cm than 100-200 cm. Key words: Bogor Botanical Garden, diversity, epiphyte bryophyte, Gymnospermae.
Keyword: 

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
Keyword: