Judul: Kajian Desain Taman Rumah Tinggal Tradisional Lampung
Abstrak: Taman rumah tinggal tradisional dapat menjadi identitas dari suatu budaya. Guna memperoleh gambaran yang nyata perlu dilakukan kajian mengenai desain taman rumah tinggal tradisional berbasis budaya Lampung. Penelitian ini mengidentifikasi elemen-elemen pembentuk taman rumah, tata letak, dan maknanya serta menyusunnya kedalam konsep desain taman rumah tinggal tradisional berbasis budaya Lampung. Penelitian dilakukan dengan menggunakan metode deskriptif melalui penelusuran informasi sejarah-kebudayaan dan diverifikasi melalui survei lapang. Observasi lapang dilakukan di beberapa wilayah Provinsi Lampung sebagai referensi, yakni Permukiman Tradisional Kampung Wana dan Sukadana Darat, Pekon Kenali, dan Negara Tulang Bawang. Halaman pada rumah tinggal tradisional Lampung dibagi menjadi tiga bagian, yang terdiri dari halaman depan atau tengahbah/terambah/beruan, halaman samping atau kebik/kakebik, serta halaman belakang atau kudan/juyu/kebon. Elemen-elemen pembentuk taman rumah tinggal tradisional adalah gakhang hadap, walai, lokasi dapur terbuka, tempat kayu bakar, dapur luar, kandang ternak, serta tanaman.
Keyword: taman tradisional, budaya Lampung, taman rumah, konsep taman, tata ruang

Judul: Kajian Desain Taman Rumah Tinggal Tradisional Budaya Sunda
Abstrak: Sundanese culture is one of the oldest culture in Indonesia. The study of traditional Sundanese garden needs to be done in order to get a real image about traditional Sundanese culture. The objectives of this research are to identify the elements, layout, and interpretation of sundanese garden. This research was obtained through descriptive analysis by field observation and tracing the information of culture and history. The study area are Urug Village, Bogor District; Sindang Barang Village, Bogor District; and Naga Village, Tasikmalaya District. The result of this study showed the spatial order of traditional Sundanese house divided into three parts in according the Tritangtu concept; those are the front or upper part, the central part, and back or under part. Yard of Sundanese house divided into three parts, those are the frontyard (buruan), sideyard (pipir), and the backyard (kebon). The dominant open space is an expanse of land or a grass. The forming elements of traditional sundanese garden are leuit, saung lisung, traditional bathroom (tampian), pond (balong), garden light (obor), cage of hen, and plants.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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