Judul: Perancangan Primer untuk Pengembangan Sistem Deteksi Berbasis PCR (Polymerase Chain Reaction) pada Ganoderma spp
Abstrak: Ganoderma spp. has been known a pathogenic fungus for palm oil. The aim of this research was to design specific primers and test it to Ganoderma spp. in order to obtain new detection system base on PCR method. Two pairs of primers were designed successfully; Gan-ITS12 primer derived from ITS region and β-Gan primer derivied from β-tubulin region. This two pairs primers were used to identified DNA from mycellia Ganoderma spp. from two different sample sources i.e IPBCC with code 10.658 (GIPB) and three different of Ganoderma sp. from Lombok (Glom1, Glom2, Glom3). The result indicates both Gan-ITS primer and β-Gan primer can amplify Ganoderma spp. on species and genus level respectively. Specifity of primers were tested to DNA from Basidiomycota group and non-Basidiomycota group. The results showed this two pairs primer can not amplification DNA from Basidiomycota group. But, this primers can amplified non-Basidiomycota groups even with more than one band formed.
Keyword: Ganoderma sp., ITS, PCR, β-tubulin

Judul: Identifikasi Ganoderma spp. Menggunakan Teknik DNA Barcoding
Abstrak: Oil palm is one of comodity plantation which is very important. One of the problems faced by the oil palm plantations are disease, namely Basal Stem Rot (BSR) which caused by Ganoderma spp. If plants were attacked by BSR, sooner or later, they would die and the production will decreased significantly. Consequently, in order to have an effective control of Ganoderma, an accurate identification is needed. This could be achieved by molecular techniques through DNA Barcoding method. The aim of this study was to is to find a specific primer for the identification of Ganoderma spp. that attack estate crops and their shade trees. Based on this research, it showed that the primer pair ITS1_F/ITS4_B has a high percentage of the ability to identify isolates of Ganoderma spp. compared with the other primer pairs, which are primer BenA F/ BenA_R, Gan_ITS1_F/Gan_ITS1_R, CO1_PenF1/CO1_PenR1, CO1_B_F/ CO1_B_R, and Gan_ITS2_F/Gan_ITS2_R. The primer pair ITS1_F/ITS4_B had a potential as a DNA barcode for identification of Ganoderma species.
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Judul: Distribusi Massa Air Laut Jawa dari Data Hidrografi MAJAFLOX Cruise 2015.
Abstrak: Laut Jawa merupakan perairan dangkal yang dipengaruhi oleh Angin Muson dan Arlindo Selat Makassar. Pemahaman tentang stratifikasi dan distribusi massa air Laut Jawa dapat dilakukan dengan adanya data hidrografi dari MAJAFLOX Cruise pada bulan Agustus 2015. Data hidrografi didapatkan dari penurunan CTD di 14 stasiun pengamatan. Hasil penelitan menunjukkan bahwa stratifikasi vertikal massa air dicirikan oleh lapisan homogen yang kuat dari permukaan hingga dasar perairan. Hal tersebut terjadi akibat percampuran massa air yang kuat oleh aksi gelombang yang dibangkitkan Angin Muson Tenggara. Selain itu, ditemukan variasi spasial dalam arah utara-selatan, dimana karakteristik massa air di tepi selatan dan utara cukup berbeda dengan massa air di tengahnya. Massa air Laut Jawa pada puncak musim timur (Agustus) dicirikan dengan massa air yang hangat (27,18 °C – 28,11 °C), bersalinitas rendah (33,58 psu – 34,2 psu), dan ringan (21,33 kg/m3 – 22,06 kg/m3), kecuali pada stasiun 1 dan stasiun 5 yang cenderung lebih hangat dan lebih tawar dari stasiun lainnya. Pola sirkulasi permukaan menunjukkan salinitas yang relatif tinggi di bagian tengah Laut Jawa dan diduga berhubungan dengan suplai massa air Arlindo Selat Makassar. Variasi tahunan dari analisis keluaran model INDESO menunjukkan adanya stratifikasi massa air yang kuat pada musim peralihan.
Keyword: angin muson, keluaran model INDESO, MAJAFLOX Cruise 2015, pengukuran CTD, stratifikasi massa air