Judul: Kajian indeks massa tubuh (imt) dan pertambahan berat badan ibu hamil serta hubungannya dengan tumbuh kembang bayi lahir di kota ambon
Abstrak: ANNA HENNY TALAHATU. Study on Body Mass Index (BMI), Pregnancy Weight Gain and its Association with Infant Growth and Development in Ambon. Supervised by HARDINSYAH and AHMAD SULAEMAN The objective of this study was to analyze pre-pregnancy body mass index (BMI) and pregnancy weight gain and its relationship with birth weight and Apgar score. For this purposes a crossectional study design was applied for 200 giving birth mother from three hospitals and three health centers (Puskesmas) based on certain criteria of 18-35 yr, complete medical records. Data collected include medical record, maternal nutrition knowledge, food habit and socio-economic. The results show that 31% of pre-pregnant women was categorized as underweight. Mean weight gain is 12,6 ± 2,4 kg and weight gain by trimester was 1,9 ± 0.6 kg , 4.2 ± 1.1 kg and 6.5 ± 1.6 kg for the first, second and third trimester respectively. Mean birth weight was 2700 ± 617.9 gr, and mean birth length is 47,5 ± 3,6 cm. About 47.5% of the newborn infants were low birth weight and 20% had low Apgar score. BMI, anemic status, pregnancy distance, morbidity, socioeconomic, and maternal nutrition knowledge had a positive significant association with total weight gain (R2 = 0.489). Based on multiple linear regression analysis, the birth weight was associated with BMI, pregnancy weight gain, gestational age, anemic status, blood pressure,and upper arm circumference (R2 = 0.734); and the Apgar score was associated with pregnancy weight gain, anemic status, blood pressure, pregnancy complication, birth weigth especially low birth weight (R2 = 0.820).
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Judul: Hubungan pertambahan berat badan ibu hamil dengan berat lahir bayi
Abstrak: Penelitian ini bertujuan mempelajari hubungan pertambahan berat badan ibu hamil dengan berat lahir bayi. Desain penelitian adalah cross sectional study, melibatkan 37 ibu hamil trimester III di Kecamatan Cibungbulang, Kabupaten Bogor. Data karakteristik contoh dan keluarga, kebiasaan makan, antenatal care (ANC), pertambahan berat badan (BB) selama hamil, status gizi sebelum hamil, paritas, jarak kehamilan, berat lahir bayi (BLB) dan usia kehamilan dikumpulkan melalui kuesioner. Hampir seluruh contoh berusia antara 20-35 tahun, dua per lima berpendidikan sekolah menengah pertama, tiga per lima berpendapatan cukup, separuh tergolong keluarga kecil dan tiga per empat contoh berstatus gizi normal dengan kebiasaan 3 kali makan utama dan 3-4 kali makanan selingan. Rata-rata ANC contoh 11 kali dan rata-rata pertambahan BB kelompok overweight (8.6±6 kg) lebih rendah dibandingkan kelompok underweight (9.3±2.5kg) dan normal (9.4±4.1kg). Hampir seluruh contoh berparitas multipara, empat per lima contoh mempunyai jarak kehamilan >3 tahun dan dua per tiga bayi dilahirkan preterm dengan BLB normal. Tidak terdapat hubungan antara pertambahan BB dengan BLB (p=0.065 dan r= 0.306). Walaupun demikian, terdapat hubungan signifikan positif antara kebiasaan makan dengan pertambahan BB dan hubungan signifikan negatif antara besar keluarga dengan kebiasaan makan (p<0.05).
Keyword: Antenatal care, birth weight, eating habit, maternal weight gain, maternal nutritional status

Judul: Isolasi Konstruksi dan Rekombinasi Gen Pengkode Reverse Transcriptase asal Simian Retrovirus-2 (SRV-2)
Abstrak: The availability of reverse transcriptase enzym as a valuable component for Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique has been providing by foreign companies. Therefore, it needs more cost and time to provide it. This study is an early step to produce in house reverse transcriptase rekombinant enzym from SRV-2. The purpose of this study was isolated, constructed, and rekombined SRV-2 reverse transcriptase gene to entry clone and destination vector. Isolation was done by PCR amplification technique using three pairs of primers, which are RT I (3282-3683), RTII (3261-3900), and RTIII (3169-4133). Construction and recombination in this study used Gateway method from Invitrogen. SRV-2 reverse transcriptase gene was isolated in this study with A-549 cell inoculated SRV-2 as source of virus. This gene amplify by PCR technique and resulted specific band for RTI 401 bp, RTII 639 bp, and RTIII 964 bp. Analysis of pENTR/SD/TOPO and pDEST17 indicated SRV-2 reverse transcriptase gene code has already inserted to this plasmid. This result indicated SRV-2 reverse transcriptase gene have isolated, constructed, and recombined to entry clone and destination vector.
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