Judul: Ageism, generativitas, dan pencapaian tugas perkembangan lansia duda dan janda
Abstrak: Pada setiap tahap kehidupan manusia termasuk tahap usia lanjut (lansia) terdapat tugas perkembangan yang harus diselesaikan dan menuntut lansia untuk beradaptasi dengan berbagai perubahan baik dari dalam diri sendiri maupun dari lingkungan sosial. Penelitian ini bertujuan untuk menganalisis pengaruh ageism dan generativitas terhadap tugas perkembangan lansia duda dan janda. Desain penelitian yang digunakan adalah cross-sectional study. Penelitian dilakukan di Kota Bogor tepatnya di Kelurahan Empang, Kecamatan Bogor Selatan dan Kelurahan Cilendek Barat, Kecamatan Bogor Barat. Sebanyak 60 orang dilibatkan sebagai contoh penelitian, terdiri dari 30 orang lansia duda dan 30 orang lansia janda yang dipilih dengan teknik snow-ball. Hasil penelitian menunjukkan tidak terdapat perbedaan nyata skor pencapaian tugas perkembangan lansia duda dan janda. Ageism berada pada kategori rendah sedangkan generatvitas dan tugas perkembangan pada kategori sedang. Terdapat hubungan dan pengaruh positif signifikan lama pendidikan dan generativitas terhadap tugas perkembangan lansia duda dan janda.
Keyword: Ageism, Elderly widow, Elderly widower, Developmental task, Generativity, Snowball method, Latter adulthood

Judul: Pengaruh Motivasi, Aktivitas, Dan Pemenuhan Tugas Perkembangan Terhadap Kepuasan Hidup Lansia Duda Dan Janda
Abstrak: Lanjut usia yang mempunyai motivasi tinggi untuk melakukan aktivitas sesuai kemampuannya akan merasa puas dalam kehidupannya. Penelitian ini bertujuan untuk menganalisis pengaruh motivasi, aktivitas sehari-hari, pemenuhan tugas perkembangan terhadap kepuasan hidup lansia duda dan janda. Desain penelitian yang digunakan adalah cross-sectional study. Penelitian bertempat di Kelurahan Empang, Kecamatan Bogor Selatan dan Kelurahan Cilendek Barat, Kecamatan Bogor Barat. Sebanyak 60 orang contoh diambil dengan teknik snow-ball yang terdiri dari 30 lansia berstatus duda dan 30 lansia berstatus janda. Lansia janda mempunyai pencapaian aktivitas lebih baik dibandingkan lansia duda. Hasil penelitian menunjukkan terdapat dua faktor yang mempengaruhi kepuasan hidup yaitu lama pendidikan dan tugas perkembangan.
Keyword: aktivitas, kepuasan hidup, motivasi lansia, tugas perkembangan

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable