Judul: Komposisi Jenis dan Struktur Tegakan Arboretum Fakultas Kehutanan dan Lingkungan IPB
Abstrak: Ekosistem hutan terdiri dari komunitas pohon dan sumber hayati lainnya yang saling terhubung satu sama lain dan dengan lingkungannya. Hubungan ini dapat dilihat melalui jumlah variasi jenis tumbuhan dan struktur tumbuhan tersebut. Tujuan dari penelitian ini adalah mendeskripsikan komposisi jenis tumbuhan dan menganalisis struktur tegakan di Arboretum Fakultas Kehutanan dan Lingkungan IPB. Metode yang digunakan adalah metode kuadrat (petak). Analisis data meliputi analisis vegetasi, dan analisis nilai Indeks Nilai Penting (INP). Hasil penelitian menunjukkan bahwa komposisi jenis pada tegakan hutan Arboretum Fakultas Kehutanan dan Lingkungan IPB didominasi oleh jenis Arthropyllum diversifolium, Calophyllum soulattri, Maesopsis eminii, Khaya anthotheca pada tingkat pohon dan permudaannya dan, Piper aduncum, dan Dracaena Fragrans pada tingkat tumbuhan bawah. Jumlah jenis pohon berikut permudaannya dan tumbuhan bawah berjumlah sebanyak 48 jenis. Stratifikasi tajuk tegakan Arboretum Fakultas Kehutanan dan Lingkungan IPB didominasi oleh stratum C dengan struktur tegakannya berbentuk huruf J terbalik., Forest ecosystems consist of communities of trees and other biological resources that are interconnected with each other and with their environment. This relationship can be seen through the number of variations in plant species and plant structure. The purpose of this study was to describe the composition of plant species and to analyze the structure of the stands in the Arboretum of the Faculty of Forestry and Environment, IPB. The method used is the quadratic method (plots). Data analysis includes analysis of vegetation, and analysis of Important Value Index (INP) values. The results showed that the species composition of the Arboretum forest stands of the Faculty of Forestry and Environment IPB was dominated by the species Arthropyllum diversifolium, Calophyllum soulattri, Maesopsis eminii and, Khaya anthotheca at trees with their regeneration, Piper aduncum, and Dracaena Fragrans at undergrowth. The total number of species for trees with their regeneration and undergrowth is 48 species. The canopy stratification of the Arboretum of Faculty of Forestry and Environment of IPB is dominated by stratum C with an iverted J shaped stand structure.
Keyword: arboretum, type composition, stratum, structure

Judul: Demonstration of Species Composition,  Stand Structure, Biomass, and Carbon Storage in Stands at the Bamboo Arboretum,  IPB Dramaga Campus
Abstrak: Ekosistem hutan dapat menyerap gas rumah kaca dengan cara menyerap CO2 dari udara menjadi simpanan karbon dalam vegetasi. Arboretum Bambu Kampus IPB Dramaga menjadi salah satu ruang terbuka hijau di dalam kampus dalam upaya mengurangi pencemaran lingkungan kota. Tujuan penelitian ini mendeskripsikan komposisi jenis, struktur vegetasi, menduga potensi biomassa, simpanan karbon, dan serapan karbon dioksida (CO2), serta mendeskripsikan sifat-sifat tanah di Arboretum Bambu Kampus IPB Dramaga. Metode yang digunakan yaitu pengukuran diameter dan tinggi (pohon, tiang, pancang, kayu mati, dan pohon mati) serta pengukuran kadar air tumbuhan bawah, semai, serasah, dan ranting. Pengambilan data tanah dilakukan dengan metode purposive sampling. Hasil penelitian menunjukkan komposisi jenis pada tegakan hutan di Arboretum Bambu Kampus IPB Dramaga didominasi oleh jenis Hevea brasiliensis (zona karet dan zona Dipterocarpaceae), Bambusa blumeana (zona bambu), dan Swietenia mahagoni (zona campuran). Kecuali zona bambu struktur horizontal tegakan pohon di Arboretum Bambu Kampus IPB Dramaga berbentuk huruf “J” terbalik. Stratifikasi tajuk hutan di Arboretum Bambu Kampus IPB Dramaga didominasi oleh stratum C. Zona karet menjadi zona dengan nilai tertinggi biomassa (112,54 ton/ha), simpanan karbon (52,89 ton/ha), dan serapan karbon dioksida (196,67 ton/ha). Kandungan C-organik tanah di Arboretum Bambu Kampus IPB Dramaga pada zona Dipterocarpaceae, zona karet, dan zona bambu termasuk tinggi, sedangkan pada zona campuran termasuk sedang.
Keyword: Biomass, carbon dioxide uptake, carbon storage, species composition, stand structure

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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