Judul: Evaluation of the Suitability of Ecological
and Aesthetic Functions of Two Program Pengembangan Kota Hijau Parks in
Bekasi City
Abstrak: Kota Bekasi mengalami penurunan kualitas lingkungan akibat kepadatan penduduk dan pembangunan infrastrukturnya. Taman sebagai ruang terbuka hijau dapat membantu mengatasi permasalahan ini. Taman Hijau Pekayon dan Taman Bantargebang merupakan salah satu hasil dari Program Pengembangan Kota Hijau di Kota Bekasi yang sudah diresmikan cukup lama. Penelitian ini bertujuan untuk mengidentifikasi jenis dan fungsi tanaman, mengevaluasi fungsi ekologis dan estetika, serta memberikan rekomendasi perbaikan pada kedua taman tersebut. Metode analisis KPI dan THI digunakan untuk menilai fungsi ekologis, sementara metode SBE digunakan untuk menilai fungsi estetika taman. Persepsi pengunjung juga dijadikan sebagai bahan pembanding dari hasil analisis yang dilakukan. Hasil penelitian menunjukkan bahwa Taman Hijau Pekayon memiliki 17 spesies tanaman dengan 61 individu, sedangkan Taman Bantargebang memiliki 16 spesies dengan 60 individu. Secara keseluruhan, Taman Hijau Pekayon memiliki fungsi ekologis yang lebih baik, sementara Taman Bantargebang lebih baik dalam fungsi estetikanya. Rekomendasi yang difokuskan dari fungsi ekologis pada kedua taman mencakup penambahan strata tanaman di tepi taman untuk meningkatkan fungsi penahan angin dan peredam bising serta penambahan tanaman penaung untuk menurunkan nilai THInya. Rekomendasi penambahan tanaman display dan fasilitas diberikan untuk meningkatkan estetika pada lanskap dengan nilai SBE rendah. Rekomendasi dibuat dengan gambar ilustrasi dan deskripsi pada kedua taman., Bekasi City is experiencing a decline in environmental quality due to population density and infrastructure development. Parks as green open spaces can help overcome this problem. Taman Hijau Pekayon and Taman Bantargebang are one of the results of the Program Pengembangan Kota Hijau in Bekasi City which has been inaugurated for a long time. This study aims to identify the types and functions of plants, evaluate ecological and aesthetic functions, and provide recommendations for improvements to the two parks. The KPI and THI analysis methods were used to assess the ecological function, while the SBE method was used to assess the aesthetic function of the park. Visitors' perceptions are also used as a comparison of the results of the analysis conducted. The results showed that Taman Hijau Pekayon has 17 plant species with 61 individuals, while Taman Bantargebang has 16 species with 60 individuals. Overall, Taman Hijau Pekayon has a better ecological function, while Taman Bantargebang is better in its aesthetic function. Recommendations focused on ecological functions in both parks include the addition of plant strata at the edge of the park to improve its windbreak and noise-canceling functions and the addition of shade plants to reduce its THI value. Recommendations for the addition of display plants and facilities were given to improve aesthetics in landscapes with low SBE scores. Recommendations were made with illustrative drawings and descriptions of both parks.
Keyword: Evaluasi, ekologis, estetika

Judul: Evaluation of the Program Pengembangan Kota Hijau in Bekasi
Abstrak: Bekasi merupakan kota dengan penduduk yang padat dan perkembangan industri yang pesat mengakibatkan berkurangnya persentase ruang terbuka hijau (RTH). Persentase yang ditetapkan sebagai minimal ketersediaan RTH setiap kota adalah 30% dari luasan kota sesuai amanat Undang-Undang Nomor 26 Tahun 2007. Pemerintah Kota Bekasi menanggapi kurangnya persentase RTH dengan bergabung dengan Program Pengembangan Kota Hijau atau P2KH. Tujuan dari penelitian ini adalah mengevaluasi penerapan P2KH di Kota Bekasi. Metode yang digunakan dalam penelitian ini adalah analisis spasial dengan remote sensing dan survei lapang serta persepsi masyarakat dengan atribut kota hijau pada skala taman. Hasil penelitian menunjukkan, kawasan terbuka hijau cenderung mengalami penurunan, pada tahun 2001 seluas 42,43%, pada tahun 2011 seluas 21,91%, dan tahun 2021 hanya seluas 9,8%. Oleh karena itu, upaya pemerintah untuk meningkatkan RTH dengan P2KH belum memenuhi amanat Undang-Undang Nomor 26 Tahun 2007. Sementara penerapan atribut kota hijau di taman, yaitu Taman Bantargebang memiliki nilai 62,37% dan Taman Hijau Pekayon memiliki nilai 46,43%, menunjukkan penerapan belum sepenuhnya diterapkan dengan baik., Bekasi is a city with dense population and rapid industrial development have resulted in decrease percentage of Green Open Space (GOS). The percentage set as the minimum for the availability of GOS in each city is 30% of the city area as mandated by Undang-Undang Nomor 26 Tahun 2007. The Bekasi City Government responded to the lack of GOS percentage by joining the Program Pengembangan Kota Hijau or P2KH. The purpose of this study is to evaluate the implementation of P2KH in Bekasi City. The method used in this research is spatial analysis with remote sensing and field surveys, and community perceptions with green city attributes at the park scale. The results showed that green open areas tend to decrease, in 2001 it had an area of 42.43%, in 2011 it had an area of 21.91%, and in 2021 it was only 9.8%. Therefore, the government's efforts to increase GOS with P2KH have not been able to fulfill the mandate of Undang-Undang Nomor 26 Tahun 2007. While the application of green city attributes in parks, namely Bantargebang Park has a value of 62.37% and Hijau Pekayon Park has a value of 46.43%, indicates that the application has not been fully implemented properly.
Keyword: green city, green open space, land cover change, spatial analysis, P2kh

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
Keyword: