Judul: Telaah sifat alergenisitas protein udang putih (Penaeus marguensis) untuk produksi isolat alergen
Abstrak: Udang merupakan bahan pangan asal laut dengan kandungan gizi mayoritas berupa protein. Kandungan protein yang tinggi selain berpengaruh positif juga dapat menimbulkan pengaruh negatif, apabila mengkonsumsinya. Pengaruh negatif konsumsi udang adalah timbulnya reaksi alergi. Senyawa penyebab alergi disebut alergen, sementara alergen umumnya adalah protein. Bagi para penderita alergi, diagnostik alergi terhadap udang memerlukan protein udang murni yang bersifat alergen. Protein alergen murni ini digunakan dalam uji alergi yang disebut uji kutanus. Penelitian ini dilakukan sebagai upaya untuk mengetahui sifat alergenisitas protein udang putih, sehingga pada akhirnya dapat digunakan untuk mengisolasi protein alergen untuk keperluan diagnostik. Isolat yang diperoleh diujikan pada serum 3 orang subyek penderita alergi terhadap udang, yaitu subyek A, Tdan Y. Ekstraksi terhadap protein sarkoplasma dan miofibrilar, dilakukan dengan مرد melarutkan diatas titik isoelektriknya, yaitu pada pH 7.5. Sebagai pelarut digunakan buffer fosfat kekuatan ion 0.05 untuk ekstraksi protein sarkoplasma dan KCl-buffer fosfat kekuatan ion 0.5 untuk protein miofibrilar. Protein yang diperoleh merupakan protein dalam larutan buffer. Pengukuran kadar protein dengan metode Bradford, baik sarkoplasma maupun miofibrilar menunjukkan bahwa kadar protein sarkoplasma dalam larutan ekstrak lebih tinggi (5.59 mg/ml) dibandingkan dengan protein miofibrilar (4.76 mg/ml). Berdasarkan hasil pengukuran kadar protein, rendemen yang akan dihasilkan adalah sebesar 10.6% pada protein sarkoplasma dan 9.05% adalah rendemen protein miofibrilar. Dari elektroforesis protein sarkoplasma maupun miofibrilar dapat diperoleh 11 pita protein. Protein sarkoplasma terdiri dari protein dengan kisaran berat molekul 17.8 KD - 74.9 KD dan protein miofibrilar dengani kisaran berat molekul 16 KD-81.1KD. ...
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Judul: Isolasi dan karakterisasi protein ikan tongkol (Auxis thazard), kerang hijau (Perna viridis) dan udang jerbung (Penaeus merguiensis) untuk pembuatan isolat alergen
Abstrak: Seafoods including fish, crustaceans and shellfish play an important role in human nutrition. However, it is also recognised as an important cause of food allergies, especially in coastal countries, including Indonesia. Treatments of these diseases still rely on avoiding the sources of the alergens, however it can also cause malnutrition which is also dangerous to health. To determine the specific food protein causing allergic reactions, cutie test was performed using isolate food protein. Until the present time, Indonesia still imports kits containing isolate proteins for food allergy diagnosis, therefore effort to locally produce the kit will reduce health cost for patients with allergic diseases. The objective of this study was to produce seafoods protein extract that is potential to be used as isolate of allergens for food allergy diagnosis. Two extract sarcoplasmic and myofibril protein were obtained from fish, shrimp and mussel by extraction in phosphate buffer with diference ionic strength Protein profile were then detected by means of sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE). IgE binding pattern was analyzed by using ELISA and immunoblotting using sera from 20 respondents with histories of food allergy. The result of the study showed that samples of seafood extract protein consist of several protein with various molecular weight in the range about 14 to 143 kDa. The component were identified as tropomyosin (34-38 kDa), parvalbumin (~12 kDa), myosin light chain (~17.5 kDa) and arginin kinase (40 kDa) which are major allergens in seafoods. Further analysis with ELISA showed that the seafood protein extracts contained several IgE-binding proteins. Immunoblot of three extract samples from seafoods showed that components identified as allergens to each allergic subject have different molecular weight. The results demonstrated that crude extract could be directly used as isolate allergen for diagnostic test allergy (skin prick test) without isolating the major allergen components.
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Judul: Implementation of Spatio-Temporal Change Query in Relational Database.
Abstrak: Geographic object changes in various views. Object changes are recorded using a model of static storage. No historical connection between old version data and new version data has been built. For that, a storage model of spatio-temporal queries that can analyze spatio-temporal changes has been developed. The object changes the geometry are grouped according to the taxonomy of Spatio-Temporal Pattern Change. This research has been able to apply the query patterns change including born, expand, merge, contract, split, normal and die. This query based on geometric (area) changes. The implementation of spatio-temporal change queries represents an object changes continuously with easy, change detection is done by comparing two versions of data. Keyword: spatio-temporal change query, spatio-temporal change pattern.
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