Judul: Pengaruh Kelekatan Remaja dengan Ibu, Ayah, dan Teman Sebaya terhadap Kenakalan Remaja di Lembaga Pembinaan Khusus Anak (LPKA) Kelas II Bandung
Abstrak: Penelitian ini bertujuan untuk menganalisis pengaruh kelekatan remaja pada ayah, ibu, dan teman sebaya terhadap kenakalan remaja di Lembaga Pembinaan Khusus Anak Kelas II Bandung. Populasi dalam penelitian ini adalah 157 narapidana remaja yang disebut andikpas (anak didik lapas) dengan usia 15-18 tahun. Contoh terdiri dari 63 andikpas yang dipilih secara simple random sampling dari LPKA Kelas II Bandung. Pengambilan data dilakukan dengan teknik wawancara dengan bantuan kuesioner. Inventory of Parent and Peer Attachment (IPPA) versi revisi (Armsdern dan Greenberg 1987) dan Adapted Self Report Delinquency Scale (ASRDS) (Pechorro, Maroco, Barroso, and Goncalves 2015) digunakan dalam penelitian ini. Hasil penelitian menemukan hubungan positif signifikan antara lama pertemanan dengan kelekatan remaja pada teman sebaya. Remaja yang lekat pada ayah dan ibu juga lekat dengan teman sebayanya, selanjutnya semakin lekat remaja dengan ayah dan ibu dapat menurunkan tingkat kenakalan remaja. Hasil uji pengaruh menunjukkan bahwa kenakalan remaja dipengaruhi secara negatif oleh kelekatan remaja dengan ayah dan dipengaruhi secara positif oleh kelekataan remaja dengan teman sebaya.
Keyword: Andikpas, kelekatan remaja dengan ibu, kelekatan remaja dengan ayah, kelekatan remaja dengan teman sebaya, kenakalan remaja.

Judul: Komunikasi Orang Tua-Remaja, Self-Esteem, dan Tingkat Kenakalan pada Remaja di Lembaga Pembinaan Khusus Anak (LPKA) Kelas II Bandung
Abstrak: Kenakalan adalah penyimpangan terhadap norma-norma yang berlaku di masyarakat (norma agama, etika dan peraturan sekolah). Meliputi pencurian, pelanggaran peraturan di jalan raya, alkohol dan obat terlarang, perusakan, pelanggaran peraturan di sekolah, dan tingkat yang mengganggu masyarakat. Penelitian ini bertujuan untuk menganalisis komunikasi orangtua-remaja, selfesteem, dan tingkat kenakalan, hubungan antara komunikasi orang tua-remaja dan self-esteem dengan tingkat kenakalan remaj, serta mengetahui variabel yang memeiliki pengaruh terhadap tingkat kenakalan remaja. Penelitian dilakukan di Lembaga Pembinaan Khusus Anak (LPKA) kelas II Bandung dan melibatkan 63 orang remaja laki-laki di LPKA kelas II Bandung dengan usia 15-18 tahun yang dipilih dengan menggunakan simple random sampling. Pengambilan data dilakukan dengan teknik wawancara dengan bantuan kuesioner. Data dianalisis dengan analisis deskriptif, korelasi, dan regresi. Hasil penelitian menunjukan hubungan negatif signifikan antara self-esteem dan komunikasi orang tua remaja dengan kenakalan remaja. Self-esteem remaja memiliki memiliki pengaruh negatif signifikan terhadap kenakalan remaja.
Keyword: Kenakalan remaja, komunikasi orang tua-remaja, self-esteem, adolescent prisoner.

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable