Judul: MSTN Gene Polymorphism of SNP c.4957C>G in Bali Cattle using PCR-RFLP
Abstrak: Gen Myostatin (MSTN) merupakan salah satu gen yang berpengaruh terhadap pertumbuhan sapi pedaging. Penelitian ini bertujuan menganalisis keragaman gen MSTN pada sapi bali menggunakan metode PCR-RFLP. Total sampel DNA sapi yang digunakan adalah 100 ekor sapi bali jantan yang berasal dari BPTU-HPT Denpasar dan BPT-HMT Serading sejumlah masing-masing 50 ekor yang diperoleh dari Aliyya et al. (2020). Analisis keragaman gen MSTN yang dilakukan pada sapi bali menggunakan PCR-RFLP dan enzim restriksi BsrI. Analisis data yang dilakukan meliputi frekuensi genotipe, frekuensi alel, nilai heterosigositas, dan keseimbangan Hardy-Weinberg menggunakan program Popgene32. Panjang produk hasil amplifikasi fragmen gen MSTN pada exon 3 sebesar 451 bp dan satu alel (C) pada sapi bali BPTU-HPT Denpasar dan sapi bali BPT-HMT Serading. Genotipe yang diperoleh adalah genotipe CC (451 bp) dengan frekuensi alel C yaitu 1 (100%). Hasil analisis keragaman menunjukkan bahwa SNP c.4957C>G MSTN pada sapi bali monomorfik atau tidak beragam. Frekuensi alel dan genotipe tidak berada tidak dalam kondisi kesetimbangan Hardy-Weinberg karena hanya satu genotipe yang muncul., The MSTN gene is one of the genes that influence the growth of beef cattle. This study aimed to analyze the polymorphism of the MSTN gene using PCR-RFLP. DNA samples used were from BPTU-HPT Denpasar and BPT-HMT Serading 50 samples each which were obtained from Aliyya et al. (2020). The MSTN gene polymorphism analysis was carried out using PCR-RFLP and BsrI restriction enzyme. Analysis of genotype and allele frequencies, heterozygosity, and Hardy-Weinberg equilibrium were using Popgene32. The result of amplification of the MSTN gene on exon 3 was 451 bp fragments and 1 allele (C) in each sample. Only one genotype was found which was CC (451 bp) with a frequency of allele C was 1 (100%). Based on the results, it can be concluded that the MSTN gene of SNP c.4957C>G in Bali cattle was monomorphic. Allele and genotype frequency was not in Hardy-Weinberg equilibrium due to only one genotype appeared.
Keyword: gen myostatin, myostatin gene, PCR-RFLP, polimorfisme, polymorphism

Judul: Polymorphism of Myostatin Gene SNP c.400G>A  (MSTN|Alu-I) on Bali Cattle Conducted by PCR-RFLP
Abstrak: Gen myostatin merupakan gen yang mengatur pertumbuhan otot rangka pada ternak dan mutasi yang terjadi pada gen tersebut dapat menyebabkan otot ganda (double muscle). Penelitian ini bertujuan mengindentifikasi dan menganalisis keragaman SNP c.400G>A gen myostatin pada sapi bali menggunakan metode PCR-RFLP. Sampel yang digunakan merupakan sampel DNA 100 ekor sapi bali dari BPTU-HPT Denpasar dan BPT-HMT Serading serta 20 ekor sapi limosin. Keragaman SNP c.400G>A gen MSTN dilakukan menggunakan metode PCRRFLP dengan enzim restriksi Alu-I. ...dst
Keyword: myostatin, PCR-RFLP, polymorphism, SNP

Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4