Judul: Zimogram aktivitas kolagenase dari bacillus licheniformis F-11.1 dan F-11.4.
Abstrak: Bacillus licheniformis F-11.1 and F-11.4 are strains from B. lichniformis F-11 were isolated from shrimp shell waste in Palembang, Indonesia. They has poliglutamat acid mutation in the operon (PGA) and chitinase AB, so it doesn’t have chitinase activity, but high protease activity. Collagenase plays an important role in the softening of meat and the wound healing process. Precipitation enzyme can use ammonium sulfate to stabilize the enzyme, therebly reducing protein denaturation. Zimogram is an electrophoresis technique to determine the activity of an enzyme in situ and qualitative results of the gel electrophoresis. This research was conduct to analyze the activity Bacillus licheniformis F-11.1 and F-11.4 collagenase qualitatively using techniques zimogram. Analysis is performed on both isolates that grown in three growth media, ie LB + 5% collagen (LBC), ½ LB + 5% collagen (LBHC), and 5% collagen (CLG). Bacillus licheniformis F-11.1 and F-11.4 produce collagenase in the three growth media were added collagen as the substrate growth. Cell growth both isolates best on LBC media. Zimogram showed B. licheniformis F-11.1 and F-11.4 produce collagenase. It shown by the formation of clear zone on the gel due to degradation of collagen by collagenase. Isolates B. licheniformis F-11.1 produce the highest fraction of enzyme crude extract derived from LBC media is 32 fraction. Meanwhile isolates B. lichniformis F-11.4 produce the highest fraction of crude extract derived from LBHC media is 37 fraction
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Judul: Produksi Kolagenase dari Isolat Bacillus licheniformis F-11.1 dan F-11.4
Abstrak: Hydrolysis by collagenase enzyme could increase nutrition and function of food proteins. This research was conducted to produce collagenase enzyme from Bacillus licheniformis F-11.1 and F- 11.4. B. licheniformis isolate F-11.1 and F-11.4 were cultured in three types of production media, which is Luria Bertani (LB) media consisted of 5% collagen (LBC), 50% LB media consisted of 5% collagen (LBHC), and 5% collagen (CLG). The activity of collagenase, protein content, and cell turbidity of B. licheniformis F-11.1 and F-11.4 were measured every 5 hours during 50 hours incubation. The result showed that optimum collagenase production of B. licheniformis isolate F- 11.1 was reached in LBC and LBHC media with activity of 2,057 U/mg and 2,037 U/mg, respectively. Optimum collagenase production of B. licheniformis isolate F-11.4 was reached in LBC media with activity of 4,695 U/mg. Isolate F-11.4 in LBHC media possesed the optimum collagenase activity of 2,811 U/mg. CLG media gave optimum collagenase activity of B. licheniformis F-11.1 and F-11.4 at 0,807 U/mg and 0,306 U/mg respectively. These research concluded that LBC and LBHC media was the best choice for collagenase production of B. licheniformis F-11.1 and F-11.4.
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Judul: Redesain Taman Denggung sebagai Taman Kota Kabupaten Sleman berbasis Kearifan Lokal di Yogyakarta
Abstrak: Kabupaten Sleman merupakan salah satu wilayah administratif di Provinsi Daerah Istimewa Yogyakarta yang mengalami peningkatan laju pembangunan infrastruktur. Laju pembangunan infrastruktur tersebut menimbulkan dampak permasalahan di Kabupaten Sleman diantaranya, ruang publik yang semakin terbatas dan pemahaman terhadap nilai kearifan lokal yang semakin rendah. Kabupaten Sleman memiliki sebuah taman yang berlokasi di pusat pemerintahan yaitu Taman Denggung. Kondisi taman tersebut cenderung sepi kunjungan dan memiliki nilai estetika yang rendah. Oleh karena itu, taman tersebut membutuhkan desain ulang dengan berbasis nilai kearifan lokal yang diharapkan dapat menjadi sarana melestarikan nilai keistimewaan Yogyakarta ditengah perkembangan infrastruktur yang semakin modern. Konsep dasar mengadopsi filosofi poros historis Yogyakarta yang menghubungkan empat komponen keistimewaan tatanan ruang Yogyakarta. Empat komponen tersebut melambangkan perjalanan kehidupan manusia dalam teori kosmologi Jawa, yaitu Gunung Merapi, Kraton, Panggung Krapyak dan Laut Selatan. Konsep desain terinspirasi motif pakaian tradisional Yogyakarta yaitu, Batik Kawung yang memiliki filosofi sedulur papat limo pancer yaitu mensinergikan antara empat unsur pembentuk alam semesta dan manusia sebagai penyelaras keempat unsur tersebut. Hasil penelitian ini adalah rekomendasi desain yang diharapkan dapat menjadi pertimbangan bagi Pemerintah Kabupaten Sleman dalam program penataan taman kota.
Keyword: Kabupaten Sleman, Kearifan Lokal, Redesain, Taman Kota