Judul: Karakterisasi Gen Ghrelin (GHRL) pada Ayam Kampung Ayam Leher Gundul Ayam Walik dan Ayam Arab
Abstrak: Ghrelin gene (GHRL) is one of important gene that stimulates secretion of gastric acid, growth hormone and food intake. Sekuens of GHRL fragment in this study was located in promoter, exon 1 and partial intron 1. This study aimed to characterize GHRL gene especially to detect mutation sites of kampong, naked neck, walik, and arab chicken. Twenty samples were amplified to result of fragment with length of 1 050 bp. Characterization of GHRL gene was processed by BLAST-N, then MEGA 5 was used to analize the sequence data. Annealing temperature was performed at 60 ºC for 1 minute. The alignment product of eight samples by using primer forward produced sequence of 554 bp. The similarity value of GHRL gene fragment with sequence data on GenBank (AB158617) was 99%. There was found 3 mutation sites of GHRL gene fragment. However, the 554 bp of GHRL gene fragment has low power to distinguish chicken breeds since all the samples have pairwise distance based on the phylogenetic tree construction analysis.
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Judul: Karakterisasi Gen Prolaktin (PRL) pada Ayam Kampung Ayam Walik Ayam Leher Gundul dan Ayam Arab
Abstrak: Indigenous and local chicken germ plasm in Indonesia have a high potency to be developed. One of the potency that can be developed is an improvement egg production. Low egg production of those chickens can be increased by selecting the in broodiness. Broodiness is controlled by prolactin gene (PRL). Since the characteristics of PRL gene of kampung, walik, leher gundul and arab are still limited refence, therefore this study aim to investigate the characteristic of PRL gene on their. Blood samples were extracted to obtain DNA. PCR then was used to amplified the fragment of PRL. A total of 20 samples successfully amplified at annealing temperature of 60 °C for 45 seconds and then directly sequenced. DNA sequencing results were analyzed using the MEGA 5 program. PRL gene in this study had similarity of 98%-99% with the PRL gene sequence data available on GenBank. This study also shows that there were 11 point mutation of PRL gene’s fragment with the type of mutations were deletions, insertions, transitions and transvertion. Those mutation types have potency as new single nucleotide polymorphisms (SNPs).
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Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4