Judul: Persepsi, Sikap, dan Partisipasi Masyarakat Sekitar terhadap Pengelolaan Hutan Kota Srengseng, Jakarta Barat menurut Perspektif Gender
Abstrak: Srengseng Urban Forest (SUF) as a form of forest area in Jakarta is now in a good conditions, but there are some damaged facilities and vegetations caused by the communities unresponsible behaviour. This research is purposed to scale the perception, attitude, and participation level of the community about SUF function and management. The research located at Srengseng Village and used 100 responden which the proportion between male and female responden is 52:48. The research showed that the perception level of the male and female included in the category of medium, jobs and education factor formed female perception significantly. The attitude level of the male and female included in the category of very high, ages factor formed both female and male attitude significantly. The participation level of male and female included in the very low with the counseling and education factor formed male participation level significantly.
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Judul: Analisis Implementasi Kebijakan padaPengelolaan Hutan Kota Srengseng, Jakarta Barat, DKI Jakarta
Abstrak: Hutan kota adalah suatu hamparan yang tumbuhannya berupa pohon-pohon yang kompak dan rapat di dalam wilayah perkotaan baik pada tanah negara maupun tanah hak, yang ditetapkan sebagai hutan kota oleh pejabat berwenang. Pengelolaan hutan kota oleh pengelola harus sesuai dengan Permenhut Nomor P.71/Menhut-II/2009 tentang Pedoman Penyelenggaran Hutan Kota. Tujuan penelitian ini adalah mengevaluasi implementasi kebijakan yang dilakukan oleh Dinas Kehutanan DKI Jakarta sesuai dengan Permenhut terhadap pengelolaan Hutan Kota Srengseng, Kota Jakarta Barat. Analisis implementasi kebijakan dilakukan dengan metode purposive sampling untuk mencari kelompok responden dan key informan, sedangkan metode snowball sampling dilakukan untuk mencari responden dan informan. Hasil Analisis implementasi kebijakan pada pengelolaan hutan kota Srengseng seluruhnya temasuk dalam kategori baik seperti kegiatan pemeliharaan dan pemantauan terkategori dengan masing-masing nilai rata-rata 3.96. Secara keseluruhan implementasi kebijakan pada pengelolaan Hutan Kota Srengseng tergolong baik dengan nilai rata-rata 3.92 dan mengacu pada Permenhut tersebut di atas.
Keyword: analisis, hutan kota, implementasi, kebijakan, pengelolaan

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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