Judul: Ekspresi Gen HSP 70 pada Sengon (Falcataria moluccana Miq.) Resisten dan Terserang Hama Boktor
Abstrak: Pohon sengon (Falcataria moluccana Miq.) merupakan salah satu pohon serba guna yang ada di Indonesia. Keberhasilan pembangunan hutan tanaman sengon dapat dipengaruhi oleh beberapa faktor pembatas pertumbuhan, salah satunya adalah ketahanan terhadap serangan hama dan penyakit tanaman. Jenis hama yang paling umum diketahui adalah serangan hama penggerek batang (Xystrocera festiva Thombs.) atau sering dikenal sebagai boktor atau uter-uter. Secara alami tanaman sengon memiliki gen ketahanan terhadap serangan hama boktor, salah satunya yaitu gen Heat shock cognate 70 kDA protein (HSP 70). Penelitian ini bertujuan untuk menguji tingkat ekspresi gen HSP 70 secara kuantitatif terhadap kambium pohon sengon resisten dan sengon terserang hama boktor melalui teknik Real Time PCR. Nilai referensi yang dipilih berupa level ekspresi housekeeping gene menggunakan gen Actin 1 dan gen target menggunakan gen HSP 70, melalui perhitungan ΔCt = Cttarget gene – Cthousekeeping gene. Hasil menunjukkan gen HSP 70 lebih besar terekspresi pada sampel resisten dibandingkan dengan sampel terserang boktor., Sengon (Falcataria moluccana Miq.) is one of the most multipurpose trees in Indonesia. The success of sengon plantation forest development can be influenced by several growth limiting factors, one of these is resistance against pest and disease. The most common type of pest known is stalk borer (Xystrocera festiva Thombs.) or often known as boktor or uter-uter. Naturally, sengon plants have genes for resistance to boktor, one of them is the Heat shock cognate 70kDA Protein (HSP 70) gene. The study aims to test the expression of a gene HSP 70 in a quantitative manner against the cambium trees resistance and sengon attacked by boktor through Real Time PCR technique. The reference level selected housekeeping gene expression in the form of using genes Actin 1 and gene HSP 70 is used, trought calculation ΔCt = Cttarget gene – Cthousekeeping gene. Results showing that genes of HSP 70 greater expression in samples of resistance than infested by boktor.
Keyword: comparative CT method, Falcataria moluccana, HSP 70

Judul: Expression of Aquaporin Genes in Sengon (Falcataria moluccana Miq) Resistant and Affected by Boktor Pests Based on  Real-Time PCR
Abstrak: Sengon (F. moluccana) merupakan spesies tanaman cepat tumbuh (fast growing) dan memiliki nilai ekonomi yang tinggi. Salah satu permasalahan yang terjadi pada penanaman sengon monokultur adalah timbulnya serangan hama boktor (Xystrocera festiva). Secara alami tanaman sengon memiliki gen ketahanan terhadap serangan hama boktor, salah satunya adalah aquaporin. Tujuan penelitian ini adalah mengukur tingkat ekspresi gen aquaporin pada kambium pohon sengon resistan dan terserang hama boktor secara kuantitatif dengan teknik real-time PCR. Prosedur penelitian ini meliputi isolasi RNA total, sintesis cDNA, perancangan primer spesifik gen aquaporin dan aktin, optimasi eskpresi gen dengan real-time PCR, dan dianalisis menggunakan metode Comparative CT method (2 -ΔΔC T). Hasil kuantifikasi menunjukkan tingkat ekspresi gen aquaporin pada ketiga sampel sengon resistan yaitu sebesar 573,05; 2,62; dan 177,99 sedangkan sengon terserang boktor sebesar 8,04; 3,76; dan 4,67. Secara garis besar, ekspresi gen aquaporin pada sengon resistan lebih tinggi dibandingkan sengon terserang hama boktor, kecuali pada sampel 2., Sengon (F. moluccana) is a fast-growing plant species with high economic value. One problem in monoculture sengon planting is the emergence of boktor pests (Xystrocera festiva). Naturally, sengon plants have genes for resistance to boktor pests, one of which is aquaporin. This study aimed to measure the level of aquaporin gene expression in the cambium of resistant and attacked boktor pests quantitatively using the Real-time PCR technique. The research procedures included total RNA isolation, cDNA synthesis, design of specific primers for aquaporin and actin genes, optimization of gene expression with real-time PCR, and analysis using the Comparative CT method (2 -ΔΔC T). The quantification results showed the expression levels of the aquaporin gene in the three samples of resistant sengon were 573,05; 2,62; and 177,99, while sengon attacked by boktor were 8,04; 3,76; and 4,67. In general, expression of the aquaporin gene in resistant sengon was higher than that of sengon attacked by boktor, except for sample 2.
Keyword: actin, aquaporin, Falcataria mollucana, real-time PCR

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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