Judul: Produksi Oligosakarida dari Umbi Kentang Hitam melalui Hidrolisis Ekstrak Kasar α-Amilase asal Brevibacterium sp.
Abstrak: Oligosaccharides is a carbohydrate macromolecule composed of 2-10 monosaccharide residues that have benefits in the field of food and health. One way to produce oligosaccharides with enzymatic hydrolisis of starch. Black potatoes have high carbohydrate content, especially starch by 83%. Activity of amylase enzyme from raw extraction have 1.708 U/ml. Degree of polymerization of black potatoe’s starch is 10. Hydrolysis reaction of black potatoe’s starch with that enzyme gave 3 spots on TLC analysis with mobile phase n-buthanol:acetic acid:water purified (2:1:1). In HPLC analysis showed 3 main peaks that means consist of 3 compound with mobile phase acetonitrile:water (75:25). Fresh sample consist of glucose as many as 1,60% (b/v), maltose 0,36% (b/v), and maltotriose 0,46% (b/v). Freeze dried sample consist of glucose as many as 4,20% (b/v), maltose 0,21% (b/v), and maltotriose 0,30 % (b/v).
Keyword: Brevibacterium sp., α-amylase enzyme, HPLC, oligosaccharides, black potatoes

Judul: Hidrolisis Pati Umbi Tacca Menggunakan Ekstrak Kasar α-Amilase dari Brevibacterium sp. untuk Menghasilkan Oligosakarida
Abstrak: Oligosaccharides have an important function in various sectors such as healthcare, industrial, food and feed, including as functional food ingredients (prebiotics). Oligosaccharides were produced from Tacca tubers (Tacca leontopetaloides) which containing 66.65 % of starch. Tacca tubers were used from Hutan Jati, and enzymes were used α-amylase from Brevibacterium sp. which has enzyme activity of 1.78 U mL-1 . The purposes of this research are to determine the optimum conditions Tacca tuber starch hydrolysis reaction and determine the type of oligosaccharides using Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC). Optimal production was achieved at 3 % substrate concentration, enzyme and substrate ratio of 1:5 and hydrolysis time of 6-8 hours. Oligosaccharides profile analysis both using TLC and HPLC showed that the enzimatically hydrolyzed samples contained maltose, maltotriose and maltotetraose.
Keyword: High Performance Liquid Chromatography (HPLC), Thin Layer Chromatography (TLC), maltooligossacharides, Brevibacterium sp., Tacca tubers

Judul: Etno-ornitologi Burung Kancilan Flores (Pachycephala nudigula nudigula Hartert 1897) pada Masyarakat Lio di Taman Nasional Kelimutu
Abstrak: Bare-throathed whistler is one of several wild birds in Kelimutu National Park (TNKL) that is believed to have correlation with Lio Community‟s belief. The estimation of Bare-throated whistler‟s population in Kelimutu National Park was about 186.41-1668.07 individual by the density about 0.0389-0.3481 ind/ha with the sex ratio 6.2:1 that spreaded on area with height of 1100 – 1600 masl. The characteristic of Bare-throated whistler‟s habitat is a natural forest with various canopy level, high plant species diversity and high density. The habitat characteristics related to the availability of food in the form of insects, cover, safety and a nesting site. Generally the most of Lio community knew the existence of Bare-throated whistler directly or from their parents‟ stories. The perceptions of Lio Community to the Bare-throated whistler‟s existence is generally support the conservation efforts. The perceptions construct the Lio Community‟s atitude that tend to be conservative to the existence of Bare-throated whistler. The attitude shown by their behavior that not cathing the Bare-throated whistler from the TNKL‟s area or the outside. The attitude have an important role to the existence of Bare-throated whistler in Kelimutu National Park‟s area.
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