Judul: The Construction of pCAMBIA 1303-Stilbene Syntase Recombinant DNA Preventive for Rotten Root Disease of Oil Palm
Abstrak: An oil palm is one of the important estate commodities in Indonesia. Nevertheless the effort to increase its productivity has many hindrances. One of them is rotten root disease caused by Ganoderma spp. New approach to get resistence oil palm toward Ganoderma attack is to insert gene produces substance played role as an antifungi. One of genes that produces antifungi is stilbene syntase gene derived from grapes (Vitis vinifera). The purpose of this research is to cloning stilbene syntase gene on to pCAMBIA 1303 expression vector between 35S CaMV promoter. Stilbene syntase gene was cloned on to pCAMBIA 1303 expression vector then to be transformed on to Escherichia coli XL-1 Blue and the last to be inserted on to Agrobacterium tumefaciens. The result of this research shows stilbene syntase gene was successful to be cloned to pCAMBIA 1303. This result was showed by two band sized 12000 bp (pCAMBIA 1303 plasmid) and 1500 bp (stilbene syntase gene) in agarose gel after have been restricted with SpeI and NcoI enzyme.
Keyword: 

Judul: Konstruksi Gen Kitinase untuk Ketahanan terhadap Ganoderma pada Vektor Ekspresi pCambia 1303
Abstrak: Efforts to increase palm oil production face problems associated with pest and diseases caused by pathogenic fungus such as Ganoderma spp. A new approach to obtain palm oil transgenic is by inserting a gene encoding antifungi compound. One of genes is chitinase gene derived from Trichoderma. The research targets are to insert chitinase gene into expression vector pCambia 1303 between the 35S Cauliflower Mosaic Virus (CaMV) promoter and the root specific promoter. The Chitinase gene was cloned into pCAMBIA 1303 expression vector followed by transformation of recombinant plasmid into Escherichia coli XL-1 Blue and transferred into Agrobacterium tumefaciens. Results showed that the chitinase gene was successfully inserted into pCambia 1303. Restriction of the recombinant DNA using restriction enzymes Nco1 and Spe1 resulted in two bands of 1200 bp which is pCambia 1303 and of 1500 bp which is the chi gene. The recombinant DNA was successfully transformed into Agrobacterium tumefaciens using competent cell of AGLO strain. The success of transformation was analyzed by restricting recombinant DNA isolated from transformant colonies using NcoI and SpeI restriction enzymes.
Keyword: 

Judul: Transisi Fasa Materi Hadronik ke Materi Kuark pada Inti Bintang Neutron.
Abstrak: Bintang neutron terdiri dari beberapa lapisan yang disusun oleh materi nuklir yang berbeda dengan kerapatan yang semakin tinggi ketika mendekati pusat bintang. Inti luar bintang neutron disusun oleh materi hadronik (nukleon, lepton, dan hyperon), sedangkan inti dalam bintang neutron disusun oleh kuark-kuark bebas (up, down, strange) yang saling berinteraksi. Tujuan penelitian ini adalah mempelajari bagaimana transisi dari inti luar ke inti dalam bintang neutron dengan menggunakan pendekatan RMF (Relativistic Mean Field) untuk materi hadronik dan CIDDM (Confined Isospin Density Dependent Mass) untuk materi kuark. Tekanan transisi diperoleh ketika potensial kimia barion materi hadronik dan materi kuark bernilai sama. Nilai tekanan transisi dengan parameter SU6-TM1 sekitar 87 MeV/fm3 dan diperoleh massa maksimum bintang neutron yaitu 1.92M dengan jari-jari sekitar 8.62 km. Nilai tekanan transisi dengan parameter SU6-G2 sekitar 53 MeV/fm3 dan massa maksimum bintang neutron yaitu 1.88M dengan jari-jari sekitar 8.16 km. Jenis transisi fasa adalah orde satu ditandai dengan diskontinuitas dalam kerapatan energi (adanya mixed phase). Parameter-parameter pada model nuklir yang digunakan menyebabkan perbedaan kekakuan persamaan keadaan (EOS) dari materi hadronik dan materi kuark sehingga nilai tekanan transisi dari fasa hadronik ke fasa kuark juga berbeda.
Keyword: bintang neutron, persamaan keadaan, transisi fasa