Judul: Keterkaitan padang lamun sebagai pemerangkap dan penghasil bahan organik dengan struktur komunitas makrozoobentos di perairan pulau Barrang Lompo
Abstrak: Seagrass beds is one of ecosystem in coastal waters which is known for its high organic productivity. The productivity derives from litter fall, epiphyte, phytoplankton and suspended particles which trapped among seagrass. Organic material then undergoing a process of decomposition and becomes a source of nutrients. Furthermore, the nutrients will be used by seagrass itself and other aquatic organisms. Macrozoobenthos is a part of the organisms that served as a connector in material cycles and energy flow, from high-level producers to consumers in the waters. The presence of macrozoobenthos in seagrass bed, will enable to support higher fish production in coastal areas. From this starting point, these studies were conducted to analyze the connection between seagrass beds as a producer and organic matter traps and its influenced to macrozoobenthos community structure. The aim of this study is to know the effect of seagrass density toward the total organic material on the bottom substrate, to investigate the effect of seagrass density on the abundance of macrozoobenthos and to determine the influence of total organic material against macrozoobenthos abundance, as well as the rate of deposition of suspended particles (mg/cm2/day). Sampling were taken in the southeast and northeast part of Barrang Lompo Island. Result of the present study, showed that all of 2 stations in Barang Lompo Islands were dominated by 5 species of seagrasses such as Cymodocea serullata, Syringodium isoetifolium, Halophila minor, Halodule uninervis, and Thalassia hemprichii. About 70 genera of macrozoobenthos have been found in all observed stations comprised of 53 families, 7 classes, and 4 phyla. Macrozoobenthos diversity index relatively higher in seagrass beds compared to bare area. However, homogenity index in seagrass beds as well as bare area was high and was relatively homogen. Thalassia hemprichii, Cymodocea serullata and Syringodium isoetifolium are the main species in this area with high densitiy. Results of Principal Component Analysis (PCA) showed that seagrass with higher density were able to trap amount of total organic material in the substrate. Macrozoobenthos density were directly proportional with seagrass density and total organic material. Gastropods are commonly found in seagrass bed with higher density and higher total organic material. In contrast with the results mention above, bivalve are more common in bare area. Coastal waters with higher clay sediment concentration may able to trap higher suspended particles than those within sandy and silty sediment.
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Judul: Community Structure of Macrozoobenthos in Seagrass Bed of Bone Batang Island South Sulawesi
Abstrak: Research about Community Structure of Macrozoobenthos in seagrass bed has been done in Bone Batang island. The aim of this research was to investigated the interaction among benthic species and seagrass in seagrass bed as their habitat. A plastic foil (4 x 3 m) were used to collect macrozoobenthos samples. Two types of station were determined. First, seagrass bed as the main station and bare sand (unvegetated area or “blow out”) as control station. Densities of macrozoobenthos from seagrass station and bare sand as control were then compared. Result showed that the highest density of macrozoobenthos collected by plastic foil method, dominated by alpheid shrimp Alpheus macellarius (2.82 individu/m2) in seagrass station and small bivalve Antigona reticulata 1.75 individu/m2) in control station. Bray-Curtis Similarity Index and n-MDS analysis for seagrass community structure among station showed, that all stations tend to have similar seagrass species composition. In contrast with the mention above, macrozoobenthos fauna from all stations showed different species composition. Correlation Analysis using Pearson Product Moment for macrozoobenthos community structure showed that there is no correlation between seagrass density and macrozoobenthos density (r = 0.302). It has been concluded that interaction among benthic species and seagrass as their habitat were influenced by environmental factors rather than direct interaction between seagrass and macrozoobenthos.
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Judul: Pengujian Virus Newcastle Disease Gen VII Isolat Lapang Sebagai Virus Standar Pada Uji Tantang Vaksin
Abstrak: This study was conducted to determine the standard of Newcastle disease virus Gene VII field isolate as a virus challenge to vaccine test. A virus suspension that has previously been tested by HA test then titrated with EID50 using 30 Specific Pathogen Free (SPF) of 10 day old chicken embryonated eggs. In addition, chicken lethal dose50 (CLD50) test were used to determine the virus titer that could cause 50% death of the chicken population. The CLD50 test was used 25 day-old-chickens (DOC) divided into 5 groups. The virus was diluted from 10-1 to 10-8. The 104 to 10-8 dilution of NDV was infected to every group of chicken via eyedrop and intranasal with 0.1 ml/chicken dosage. Haemagglutination test showed that the allantoic fluid were contain high titers of NDV is 512 HAU. The infective NDV that had been titrated by EID50 test showed the virus titers is 109 EID50/0.1 ml or 1010 EID50/ml and the virus titers of CLD50 test e.i 106.48 CLD50/0.1 ml or 107.48 CLD50/ml. These results can be concluded that the NDV Gene VII field isolates were most appropriate to be used as a standard virus to vaccine test
Keyword: Newcastle Disease Virus Gene VII, HA tes, EID50 test, CLD50 test