Judul: Efek anabolik dan androgenik ekstrak heksana buah okra(Abelmoschusesculentus L.Moench)
Abstrak: The analyse of genetic variety was done through 14 samples of timor deer (Cervus timorensis) from South East Sulawesi and Nusa Tenggara Timur by using PCR (Polymerase Chain Reaction) tehnique. Observation method were (1) Total DNA isolation, (2) Quality of DNA evaluation, (3) Amplification of genome Mitochondrial DNA D-Loop region, (4) Cutting by restriction enzyme, (5) Analyse PCR result and its cutting and (6) Data analyse. Amplification D-Loop region of deer mitochondrial DNA was done by a pair of primers. CTDF primer (5'-TAA TAT ACT GGT CTT GTA AAC C-3') stick to threonin tRNA and CTDR primer (5'- GGC TGG CAC GAG ATT TAC CAA CCC-3') stick to 12SrRNA region. Amplification through all deer samples gave a fragment, which is sized 1340 bp. Base on reference sequence data, the fragment that's got, sized 1437 bp. There are 97 bp diversities, these were caused by sequence reference using RNA, RNA, tRNA Phe and a half of 12SrRNA came from another species, that was Muntiacus reevesi (Gene-Bank, access code NC_004069) and it's not from timor deer because the fragment basic data wasn't available. Further amplification result was cut by six kinds of restriction enzyme, which is cutting enzyme four bases HaelII (GG-CC), HapII (C/CGG), Alul (AGCT) and Rsal (GT-AC) and cutting enzyme six bases BamHI (G-GATCC) and EcoRI (GAATTC). The samples gave monomorphic cutting result, we can also say, it's not detected a variation to D-Loop region of timor
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Judul: Efektivitas Design Primer D-Loop Rusa timorensis untuk Analisis Genetika Populasi
Abstrak: Javan deer (Rusa timorensis) is an endemic species in Indonesia estimated at less than 10.000 individuals and continuously declined due to habitat loss and illegal animal hunting. This situation shows that Javan deer is facing a greater risk of extinction. Hence a conservation effort is necessary to keep the sustainability of Javan deer. This study proposes the primer design of the D-loop region to determine the genetic population of wild Javan deer. This study used Primer3 for primer design, BLAST for specificity, and Oligo AnalyzerTM for efficiency. The annealing temperature optimisation started with predenaturation at 94°C. It followed with 35 cycles of denaturation at 95°C, 51-56°C annealing, and 72°C extensions. This study has succeeded in designing a practical primer for Javan deer species with an optimum annealing temperature is 52°C. The DNA amplified in the sample storage with the two-step method has a higher percentage (59.13%) than ethanol 96% (32.35%). All sequenced samples are homolog to Rusa timorensis sequence from NCBI., Rusa jawa (Rusa timorensis) merupakan spesies endemik Indonesia yang diperkirakan kurang dari 10.000 individu dan terus-menerus mengalami penurunan karena hilangnya habitat dan perburuan hewan ilegal. Situasi ini mengindikasikan bahwa Rusa jawa mengalami risiko kepunahan yang besar. Oleh karena itu, upaya konservasi diperlukan untuk mempertahankan Rusa jawa. Penelitian ini bertujuan untuk mendesain primer daerah D-loop untuk menentukan genetik populasi Rusa jawa di alam. Penelitian ini menggunakan Primer3 untuk desain primer, BLAST untuk spesifisitas, dan Oligo AnalyzerTM untuk efisiensi. Optimasi suhu penempelan primer diawali dengan pradenaturasi 94°C. Selanjutnya diikuti dengan 35 siklus dari denaturasi 95°C, 51-56°C penempelan primer, dan 72°C pemanjangan. Penelitian ini berhasil mendesain primer yang efektif untuk spesies Rusa jawa dengan suhu optimum penempelan primer yaitu 52°C. Amplifikasi DNA pada sampel yang disimpan dengan metode dua langkah memiliki persentase lebih tinggi (59.13%) dibandingan dengan etanol 96% (32.35%). Semua sampel yang disekuensing homolog dengan sekuen Rusa timorensis dari NCBI.
Keyword: Conservation genetics, Javan deer, Mitochondrial DNA, Wild genetic populations

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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