Judul: Analisis Keberlanjutan Pembangunan Kota Depok, Jawa Barat.
Abstrak: Kota Depok merupakan bagian dari Kawasan Metropolitan Jabodetabek yang mengalami ekspansi perkotaan dan tekanan migrasi penduduk yang cukup tinggi. Namun dalam dekade terakhir terjadi ketidakseimbangan kinerja pembangunan, dimana pembangunan dalam dimensi ekonomi dan sosial cenderung lebih baik dibandingkan kinerja pembangunan dalam dimensi lingkungan. Hal ini dapat dilihat dari peningkatan fasilitas ekonomi dan fasilitas pelayanan publik, namun di sisi lain terjadi penurunan kualitas lingkungan. Capaian kinerja pembangunan berkelanjutan Kota Depok dapat diukur pada unit analisis mikro (di tingkat kelurahan), yaitu dengan penghitungan Indeks Keberlanjutan Lokal (IKL). Penelitian ini dilaksanakan di Kota Depok dari bulan Januari 2021 hingga Desember 2021 menggunakan data potensi desa Kota Depok tahun 2018, Dokumen Kecamatan Dalam Angka Kota Depok Tahun 2018, serta peta administrasi Kota Depok. Nilai IKL dianalisis dengan metode factor analysis (FA) menggunakan Statistica 7.0 dan selanjutnya dilakukan pemetaan distribusi spasialnya berdasarkan wilayah administrasi menggunakan ArcGIS. Analisis Asosiasi Spasial nilai IKL untuk masing-masing dimensi dianalisis menggunakan Indeks Moran Global dan LISA (Local Indicator of Spatial Autocorrelation) menggunakan GeoDa, dan tipologi wilayah dianalisis dengan teknik analisis cluster menggunakan Statistica 7.0. Hasil penelitian menunjukkan bahwa IKL ekonomi lebih tinggi dibandingkan dengan IKL sosial dan IKL lingkungan. Pola distribusi IKL sosial, ekonomi, dan lingkungan sebagai indikator keberlanjutan lokal tergolong acak (random). Nilai indeks Moran Global untuk dimensi sosial, ekonomi, dan lingkungan secara berturut-turut adalah 0.209, 0.229, dan 0.202 yang menunjukkan adanya autokorelasi positif yang menggambarkan pola spasial mengelompok. Hasil analisis cluster menunjukkan bahwa sebanyak 63% kelurahan masuk kedalam Cluster 1 (memiliki nilai dimensi sosial tergolong sangat rendah, dimensi ekonomi dan lingkangan tergolong sedang), sebanyak 24% kelurahan masuk kedalam Cluster 2 (memiliki nilai dimensi ekonomi tergolong tinggi, dimensi sosial dan lingkungan tergolong rendah), dan sebanyak 13% kelurahan masuk kedalam Cluster 3 (memiliki nilai dimensi sosial tergolong tinggi, dimensi ekonomi dan lingkungan tergolong sedang) dari total jumlah kelurahan yang ada di Kota Depok. Untuk selanjutnya, hasil dari penelitian ini dapat digunakan oleh pemerinta Kota Depok sebagai rekomendasi dalam menyusun kebijakan, rencana, dan/atau program dalam upaya mewujudkan pembangunan berkelanjutan di masing masing kelurahan.
Keyword: cluster analysis, factor analysis, Global Moran Index, Local Sustainability Index, LISA

Judul: Analisis Keberelanjutan Lokal Kota Depok, Jawa Barat
Abstrak: Depok City is part of the Greater Jakarta Metropolitan Area which is experiencing urban expansion and high population migration pressure. However, within last decade there has been an imbalance development, where economic and social dimensions tend to be better than the performance of environmental one. This can be seen from the increase of economic and public service facilities, in contrast to a decrease in environmental quality. The performance of Depok City's sustainable development can be measured at a micro analysis (at the kelurahan level), namely by calculating the Local Sustainability Index (IKL). This research was carried out in Depok City from January 2021 to December 2021 by using several characteristics of Depok City villages in 2018, collected from Figures for the Depok City in 2018, as well as the Depok City administration map. The IKL was computed employing factor analysis (FA) on Statistica 7.0 and then the spatial distribution was mapped based on administrative region in ArcGIS. The Spatial Association of IKL was evaluated by using Global Moran Index and LISA (Local Indicator of Spatial Autocorrelation) with GeoDa, and the typology of the region was then grouped by employing cluster analysis on Statistica 7.0. The results showed that economic IKL was higher than social and environmental IKLs. The spatial pattern of social, economic, and environmental IKL as indicators of local sustainability was somewhat random. Global Moran Index for social, economic, and environmental dimensions were 0.209, 0.229, and 0.202, respectively, which indicate positive autocorrelations describing spatially clustered pattern. The cluster analysis resulted 63% of urban villages occupied Cluster 1 (having a very low IKL for social dimension value, economic and environmental dimensions are classified as moderate value), 24% of villages occupied Cluster 2 (having a high IKL economic dimension value, social and environmental dimensions are classified as low IKL value, and 13% of villages occupied Cluster 3 (having a high IKL social dimension values, economic and environmental dimensions are classified as moderate IKL value). Furthermore, the results of this research can be used by the government of Depok City as a recommendation in formulating policies, plans, and/or programs in an effort to actualize sustainable development in each kelurahan.
Keyword: cluster analysis, factor analysis, Global Moran Index, Local Sustainability Index, LISA

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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