Judul: The Potency of Cissus quadrangula Salisb. Stem Extract on Proliferation and Differentiation of Rats Bone Marrow Mesenchymal Stem Cell into Osteocytes.
Abstrak: Cissus quadrangula Salisb. (CQ) was traditionally used in certain bone diseases treatment, in Aceh. Cissus quadrangula Salisb. contained biochemical compounds such as phytoestrogen, calcium and phosphorous, that were reported can trigered osteogenesis. Mesenchymal stem cells from bone marrow can be differentiated to be several cell types such as osteoblast, osteocyte and adipocyte. This study was conducted to observed potency and optimal dose of CQ in proliferation and differentiation of rats bone marrow mesenchymal stem cell. Mesenchymal stem cells (MSC) were isolated from os femur and os tibia of rats. There were five treatment groups in this study. All treatment groups used modified Dulbecco’s modified eagles’s medium (mDMEM) medium. In the control group was not added CQ extract, while in the other groups were added 0.1 mg/mL (CQ 0.1 mg/mL), 0.3 mg/mL (CQ 0.3 mg/mL), 0.6 mg/mL (CQ 0.6 mg/mL) and 0.9 mg/mL (CQ 0.9 mg/mL) of CQ extract. The level of cell proliferation was analyzed by using population doubling time (PDT) technique. Identification of cell differentiation was done by morphological observation after staining with Alizarin red. Cell differentiation was indicated by the number and diameter of osteoblast and osteocyte. The result showed CQ extract treatments decreased PDT significantly (P<0.01), than that of control. It showed that CQ stem extract increased proliferation of bone marrow mesenchymal stem cell. The number of osteoblast of control treatment group was lowest significantly (P<0.01) than others CQ stem extract treatment groups. The treatment of 0.3 mg/mL CQ stem extract showed the highest number of osteocyte significantly (P<0.01) than others treatment groups. It showed that CQ stem extract increased cell differentiation. The diameter of osteoblast and osteocyte of all treatments groups were not different significantly (P>0.05). It showed that CQ stem extract did not influence on cell diameter. The study concluded that CQ stem extract increased proliferation and differentiation of rats’ bone marrow mesenchymal stem cell into osteoblast and osteocyte. The optimal dose of CQ stem extract was 0.3 mg/mL.
Keyword: Cissus quadrangula Salisb, Mesechymal Stem Cell, Proliferation, Differentiation

Judul: Quantitative Analysis of Histopathology Changes in Liver, Kidney, and Spleen Tissue of Mice (Mus musculus) after Injection of Human Mesenchymal Stem Cells (hMSCs) and Conditioned Medium (CM)
Abstrak: Terapi stem cell menjadi salah satu metode pengobatan baru yang paling menjanjikan untuk mengobati penyakit degeneratif dan iskemik. Human Mesenchymal Stem Cells (hMSCs) dan Conditioned Medium (CM) dapat menghasilkan respon imun. Penelitian ini bertujuan untuk mempelajari respon jaringan hati, ginjal, dan limpa mencit (Mus musculus) melalui gambaran histopatologi setelah diberi hMSCs dan CM melalui vena coccygea. Mencit DDY jantan dengan jumlah 15 ekor dibagi menjadi tiga kelompok perlakuan, kelompok hMSCs, kelompok CM, dan kelompok kontrol yang diinjeksi normal saline. Mencit dinekropsi pada hari ke-14 dan diambil sampel organ untuk difiksasi dengan formalin 10%, kemudian dibuat preparat histopatologi dengan pewarnaan Haematoxylin Eosin (HE) dengan processing yang sama sehingga warna comparable. Gambar histopatologi di analisa secara kuantatif menggunakan aplikasi ImageJ ver. 1.50g dan R software. Hasil penelitian menunjukkan jumlah sel Kupffer menurun yang mengindikasikan tidak adanya inflamasi, hepatosit diduga mengalami regenerasi, luas ruang Bowman kelompok CM menyempit, sel tubulus kelompok hMSCs lebih mature, dan splenosit kelompok hMSCs dan CM immature. Perubahan tidak memperlihatkan adanya respon toksisitas pada jaringan hati, ginjal, dan limpa mencit., Stem cell therapy is one of the most promising new treatment methods for treating degenerative and ischemic diseases. Human Mesenchymal Stem Cells (hMSCs) and Conditioned Medium (CM) can produce an immune response. This study aims to determine the response of liver, kidney, and spleen tissue of mice (Mus musculus) through histopathology after being given hMSCs and CM through the coccygeal vein. The 15 male DDY mice were divided into three treatment groups, hMSCs, CM, and control injected with normal saline. Mice were necropsied on the 14th day and organ samples were taken to be fixed with 10% formalin, then histopathology preparations were made with Hematoxylin Eosin (HE) staining with the same processing so that the color was comparable. Histopathology images were analyzed quantitatively using the ImageJ app version 1.50 g and R software. The result showed that the number of Kupffer cells decreased which indicated the absence of inflammation, hepatocytes are thought to regenerate, Bowman’s space tightened in the CM group, tubular cells in the hMSCs group were more mature, and splenocytes in the hMSCs and CM groups were immature. The changes did not show any toxic response in the liver, kidney, and spleen tissues of mice.
Keyword: conditioned medium, human mesenchymal stem cells, kidney histopathology, liver histopathology, spleen histopathology

Judul: Mencari Aproksimasi Akar-Akar Polinomial Kompleks Dengan Algoritma Weyl
Abstrak: Mencari akarMakar polinomial dengan menggunakan algoritma Weyt. Algoritma WeyJ merupakan algoritma rekursif yang proses kerjanya dapat diana10gkan dengan algoritma Bisection. Karena algoritma Weyl bergerak dalam bidang kompleks maka memerlukan nilai awal hempa bujur sangkar. Dalam proses penyempitan bujur sangkar tersebut algoritma Weyl memerlukan algorittna Turan untuk menentukan jarak antara suatu titik dengan akar polinomial yang terdekat dengan titik tersebut. Karena apabiJa jarak tersebut temyata lebih hesar dari Yl panjang diagonal bujur sangkar yang diamati maka bujur sangkar tersebut tidak memuat satu pun akar polinomial. Untuk seJanjutnya bujur sangkar tersebut diabaikan. Sebaliknya apabila jarak yang diperoleh lebih keeil dari \6 panjang diagonal bujur sangkar yang diamati, maka bujur sangkar tersebut yang akan diperkeciL
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