Judul: Perubahan penggunaan lahan dalam hubungannya dengan aktivitas pertambangan di Kabupaten Bangka Tengah
Abstrak: This research has been performed in order to evaluate the landuse change in Bangka Tengah Regency and it association with tin mining activities and to access the pattern and driving forces of the landuse change. Multitemporal remote sensing data were used to analyze the dynamics of land use changes. Landsat satellite images of 2000, 2004 and 2010 were used to interprete ten classes of land use using the Geographical Information System (GIS) and Multiple Regression Analysis were used to access the driving force of the landuse change. The results show many changes among the land use in the year between 2000 and 2010. The highest rate of changes is observed in estate area which increased by 6 612.50 ha per year. The lowest rate of changes is observed in shrub area which decreased by 7 062.50 ha per year. Tin mining area increased by 8.1% per year (1 315 ha per year) and on the other hand forest decreased by 4.9% per year (601 ha per year) in the year between 2000 and 2010. In this case, index of regional growth and tin reserves is the significant factor that caused changes in land uses.
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Judul: Reklamasi lahan pascatambang timah dan tingkat perkembangan wilayah di Kabupaten Bangka
Abstrak: Tin mining industry in Bangka Regency contributes positively to the national income, and increase in Gross Regional Domestic Product (GRDP), labour availability, and economic value. The tin mining also poses negative impacts on the environment. Post-mined reclamation is expected to increase regional economic level and stimulate economic growth so that after the closure of mining activities there is no inequality in the community socio-economic aspect. The aims of the research are: (1) to assess tin post-mined land reclamation techniques for plant growth, (2) to assess the types of reclamation plants suitable to grow and have an economic benefit to the community, (3) to determine priority of the reclamation plant types suitable to grow based on the stakeholders perceptions, (4) to plan an arrangement of plant types and land reclamation techniques in the tin post-mined area, and (5) to determine the level of the community income and regional development in villages with tin mining activities in Bangka Regency. The results show that the reclamation technique that can be implemented was by providing soil ameliorants (manure or compost, top soil and rock phosphate or dolomite) and fertilizers to the tailings, and then provide MAF inoculant or planting cover crops of the leguminoseae familiy and planted with native plants or plants which suitable to grow in the such a tin postmined land conditions. Implementation of the reclamation technique resulted in an increase in land suitability classes to be S3 (marginally suitable) for rubber (Hevea brasiliensis) and S2 (moderately suitable) for sengon (Paraserianthes falcataria) and acacia (Acacia auriculiformis). Based on the stakeholder perception, rubber is the priority plant, while plant which generate economic benefit is sengon. The community income of villages around the tin mining area increased in the period of 2003-2008. At the time of mining activities, hierarchy of some villages around the mine increased but then decreased after the closure of the tin mining activities.
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Judul: Excretory/secretory antigen of ascaridia galli L3 stage to trigger yolk immunoglobulin in laying hens
Abstrak: Ascaridiosis caused by the nematode Ascaridia galli. A study was carried out to characterize excretory/secretory antigen of A. galli from larval stage to trigger anti-ascaridiosis yolk immunoglobulin in laying hens. A. galli L3 were recovered from intestines of 100 heads chickens 7 days after oesophagus inoculation with 6000 L2. L3 recovered in this manner were cultured (5 – 10 ml-1) in flasks containing rosswell park memorial institute (RPMI) 1640 media, pH 6.8, without phenol red. Cultures were incubated at 370C in 5% CO2 and culture fluid was collected after 3 days in culture. To prepare excretory/secretory antigen, culture medium were concentrated with vivaspin 30.000 MWCO, and antigen protein concentration were counted as described in Bradford method. The molecular weight of excretory/secretory antigen was determined with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). Active immunizations were applied intra muscularly of chickens with an initial dose of 80 μg. The immunizations were repeated three times with dose of each 60 μg with an interval of one week. The first immunizations were excretory/secretory antigen mixed with fruend adjuvant complete and subsequently mixed with freund adjuvant incomplete. The chicken’s eggs were collected from 49 day after immunizations. Anti-ascaridiosis yolk immunoglobulin (IgY) was extracted from egg yolks by means of ammonium sulphate and purified using fast performance liquid chromatography (FPLC). The purity of anti-ascaridiosis IgY was determined by Bradford method (λ = 280 nm). Antibody was detected by agar gel precipitation test (AGPT) and enzyme linked immunosorbant assay (ELISA). Antigen were detected with immunohistochemistry. Chickens were challanged with dose of 1000 L2 A. galli. Passive immunizations were applied ten times with dose of each 0,875 mg IgY with an interval of one day intra orally. IgY anti-ascaridiosis was tested against A. galli survival in vitro.
Keyword: Unggas, Isolasi dan karakterisasi antigen, Purifikasi imunoglobulin yolk, Pengukuran antibodi ayam petelur, Metode Imunohistokimia, Ascaridiosis