Judul: Isolasi dan Identifikasi Genetik Monokarion Jamur Tiram (Pleurotus sp.)
Abstrak: One way to obtain high quality isolates in breeding programe is hybridization to increase genetic variation of offspring. The first step for hybridization was monokaryons collection. Monokaryon is haploid primer hyphae resulted of single basidiospore germinated. The research aim to provide Pleurotus sp. monokarions which their mating type has been identified, also conduct monokaryon genotype characteritation through Amplified Fragment Length Polymorphism technique (AFLP). This research used basidocarp of one strain of Pleurotus sp. Methods in this research were consisted of: monokaryon isolation, mating type test, segregation test, and genotype variation analysis which consist of DNA isolation, and AFLP. This research successfully got 30 monokaryons which were distributed into four mating types, those are P1O1, P2O2, P1O2, dan P2O1. Two mating type genes present segregation and random assortment theory. Result of AFLP data clustering analysis showed monokaryons had two main group which could be used as considering to choose parentals with far genetic difference.
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Judul: Analisis Keanekaragaman Isolat Pleurotus spp. Liar dan Domestik Berdasar Sifat Morfologi, Fisiologi dan Molekuler
Abstrak: Oyster mushroom (Pleurotus spp.) are edible mushroom with high diversity. Cultivated oyster isolates in Indonesia are from local isolates as well as from outside Indonesia. Report on their genetic diversity based on characteristic of morphological and physiological are still scare found in Indonesia, even analytic based on molecular characteristic is never report in Indonesia. This study was aimed to identify several kind of wild and domestic isolates of oyster mushrooms based on morphological, physiological, genetic, and molecular characteristic. The result indicated that there were two groups of isolates based on morphological characteristic of a fruiting body, A group and B group. A group was consisted ofHHB247 isolate (pink color) and nine isolates (white and brown color) grouped on B which then divided into two groups B1 and B2_ This B1 divided into two groups C1 and C2. C1 included only one isolate that was HHB215 isolate (brown color ) and C2 include one white color isolate (sp2) and one brown color isolate (sp6). B2 divided two groups D1 and D2, D1 included two white color isolates (sp3, sp5) and one brown color isolate (HHB328). D2 included three white color isolates (HHB327, sp4 and spl). Based on morphological characteristic the, ten of oyster mushroom isolates can not be in groups related to the color of fruiting bodies. The result indicated that there were two groups of isolates based on physiological characteristic, A group and B group. A group was consisted of HHB247 isolate (pink color) and nine isolates (white and brown color) grouped on B which then divided into two groups B1 and B2. B1 included only one isolate that was sp6 isolate (brown color). B2 divided two groups Cl and C2. Cl included two brown color isolates (HHB215, HHB328), C2 divided two groups Dl and D2. Dl include five white color isolates (spl, sp2, sp3, sp4, and sp5), D2 included only one isolate that was HHB327 isolate (white color). Based on physiological characteristic, ten of oyster mushroom isolates can be in groups related to the color. The frequency of mating between single spores of intra isolates (similar color of fruiting bodies) was highly (60% - 74%), but the frequency of mating between inter isolates was O %. Molecular analysis on Pleurotus spp. and Lentinus isolates was done using RAPD molecular marker with five primers (RP-2, RP-3, Rp-3, RP-4 and RP-6). The result indicated that there were two groups of isolates based on moleculer characteristic, A group and B group. A divided two groups Al and A2. Al included only one isolate that was HHB307 isolate ( the color was not determined), and A2 included six white color isolates (HHB327, Spl, Sp2, Sp3, Sp4, and Sp5) with 100% of similarity index. B divided two groups Bl and B2. Bl included three brown color isolates (HHB32S, HHB215 and sp6) with two last isolates had 100% of similarity index, and B2 included one...dst
Keyword: jamur tiram, RAPD, mating, analisis molekuler, spora tunggal

Judul: Pengaruh ukuran billboard terhadap kualitas estetika lanskap kota Bogor
Abstrak: Salah satu permasalahan kota yang dialami saat ini antara lain adalah permintaan billboard. Penataan dan pemasangan billboard yang salah dapat menurunkan kualitas estetika lanskap kota. Ukuran billboard relatif besar, mendominasi pandangan sehingga menutupi good view yang berada di belakangnya.
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