Judul: Kegiatan Magang Perancangan Lanskap Kawasan Perumahan Green Permata di PT. Tropica Greeneries, Jakarta
Abstrak: Human needs a place to stay, so that a residential area should be constructed by supporting facilities. Today, residential area are dominated by building structures. Green Permata is a residential area that combines a modern tropical concept in the area of Jakarta. To realize it, they need a design process in accordance with the wishes of the owner and accordance with the aesthetics and functional value. PT. Tropica Greeneries as consultant firm as well as landscape contractors who have years experience, is trusted by PT. Agung Podomoro Land to design and build the landscape of Green Permata Residential Area. The purpose of making the Green Permata Residential area landscape design is to fulfill basic human need for place with green oasis in Jakarta city concept. The internship activities that were conducted during the four months from February until May 2012 in PT. Tropica Greeneries, Jakarta. The result of landscape design study during internship is an experience to produce site plan, image section, planting plan and hardscape plan.
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Judul: Proses Perancangan Lanskap Kompleks Gallery West, Kebun Jeruk, Jakarta Barat
Abstrak: Jakarta is a metropolitan city that plays an important role in urban development. As the center of the city, urban development has increased with the increasing of buildings and office buildings as places for businesses and economic activities. Moreover, the entertainment center and residential area such as hotels and apartment buildings began to grow rapidly, leaving limited open space area and green areas. It takes a strategy in the utilization efficiency of construction building and effectiveness for a variety of needs in an area that is well integrated. Mixed-use development is a strategy that blends a combination of residential, commercial, cultural, institutional, or industrial uses, where those functions are physically and functionally integrated, and that provides pedestrian connections. The concept of mixed-use has been widely applied in many big city in America and Europe. Mixed-use nowadays began to developed in Jakarta, especially in residential complex area. The benefits of mixed-use development include: reduced distances between housing, workplaces, retail businesses, and other amenities and destinations, walkable, bike-able neighborhoods, increased accessibility via transit, both resulting in reduced transportation costs, more compact development, land-use synergy stronger neighborhood character, sense of place etc. Oemardi_zain (OZ) is one of the landscape consultant that based on landscape planning and designing in Indonesia. The scope of landscape design covers landscape hotel, club, resort, residential, theme park, civic,dan comercial park. Internship activities conducted on one project design of Gallery West that used the concept of mixed-use in its buildings development. With a total area of ± 20.400 m² with area landscape design includes entrance area and gateway, roof garden, green connector and pool courtyard corridor, and terrace. Through several of following designing landscape process during the internship, student can learns implementing design process in landscape project, improving skills and ability in analyzing and designing in the studio. The internship involves active participation, interview with employees, direct observations on site, and literature study. The result of the internship is to learn the project implementation procedures, organizational structure of OZ, division of works, design process include: project acceptance, documentation and agreement research and analysis, concept, design development and detail constructions.
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Judul: Influenza Specific Immunoglobulin Y Purification from Egg Yolk with Sodium Chloride Precipitation Method
Abstrak: Imunoglobulin Yolk (IgY) yang terdapat dalam kuning telur telah banyak dimanfaatkan untuk imunoterapi dan imunodiagnostik. Salah satu kendala dalam pemurnian IgY dari kuning telur adalah pemisahan Ig dengan lemak, sehingga teknik pemurnian Ig selalu berkembang. Penelitian ini bertujuan untuk melakukan pemurnian IgY dari kuning telur dengan metode pengendapan menggunakan sodium chloride (NaCl) serta melakukan karakterisasi IgY spesifik influenza. Sodium chloride dipilih karena bahan kimia ini relatif murah, ramah lingkungan, dan tidak merusak imunoaktivitas IgY. Kuning telur yang digunakan dalam penelitian ini berasal dari telur ayam layer yang divaksinasi dengan vaksin influenza Flubio® yang berisi virus Influenza A/H3N2, A/H1N1 dan B/Washington. Pemurnian IgY hanya dilakukan pada kuning telur yang mengandung antibodi spesifik influenza. Deteksi keberadaan antibodi spesifik influenza ditentukan dengan uji agar gel presipitasi (AGP) dan metode enzyme linked immunosorbent assay (ELISA). Konsentrasi IgY hasil pemurnian dihitung dengan spektrofotometer dan karakterisasi protein dilakukan dengan metode Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Konsentrasi protein dari hasil pemurnian IgY memiliki nilai rata – rata 50,403 mg/mL. Hasil SDS-PAGE menunjukkan enam pita protein, yaitu struktur IgY utuh (174,63 kDa), Apoprotein-LDL (129,77 kDa), rantai berat IgY (66,54 kDa), Fab ( 47,65 kDa), rantai ringan IgY (21,85 kDa), dan β-livetin (36,74 kDa). IgY hasil pemurnian masih dapat berikatan dengan antigen influenza berdasarkan uji ELISA. Hasil penelitian ini menunjukkan bahwa pemurnian IgY menggunakan metode pengendapan NaCl dapat memurnikan IgY dengan struktur utuh, meskipun masih ada dua protein tidak spesifik hasil pemurnian. IgY yang dapat berikatan dengan antigen influenza mengindikasikan prospek pemanfaatannya untuk pencegahan influenza dan imunodiagnostik., Yolk immunoglobulin (IgY) contained in egg yolk has been widely used for immunotherapy and immunodiagnostics. One of the obstacles in IgY purification from egg yolk is separating Ig from fat, so that IgY purification techniques are always developing. This study aimed to purify IgY from egg yolk using sodium chloride (NaCl) precipitation method and to characterize influenza-specific IgY. Sodium chloride was chosen because this chemical is relatively unexpensive, environmentally friendly, and does not impair IgY immunoactivity. The egg yolks used in this study were collected from layer eggs vaccinated with the Flubio® influenza vaccine, which contains the Influenza A/H3N2, A/H1N1 and B/Washington viruses. IgY purification was only carried out on egg yolks containing influenza specific antibodies. The presence of influenza-specific antibodies was determined by the agar gel precipitation (AGP) test and the enzyme linked immunosorbent assay (ELISA) method. The purified IgY concentration was determined using spectrophotometer, and protein characterization was performed using the Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) method. Protein concentration from purification has a mean value of 50,403 mg/mL . SDS-PAGE result showed six protein bands, namely intact IgY structure (174,63 kDa), Apoprotein-LDL (129,77 kDa), IgY heavy chain (66,54 kDa), Fab (47,65 kDa), IgY light chain (21,85 kDa), and β-livetin (36,74 kDa). The purified IgY still able to bind with influenza antigen based on the ELISA test. The results of this study indicate that purification of IgY using the NaCl precipitation method can purify IgY with intact structure, although there are still two unspecific proteins in the purification results. The resulting IgY can bind to influenza antigens indicating the
Keyword: Immunoglobulin Y, influenza, egg yolk, purification, sodium chloride