Judul: Hubungan sosialisasi dalam keluarga dan lingkungan sekolah dengan tingkat kenakalan siswa sekolah (Studi kasus di SMUN 7 dan SMU PGRI4, Kotamadya Bogor, Propinsi Jawa Barat)
Abstrak: Penelitian ini bertujuan 1) Menganalisis tingkat kenakalan siswa di sekolah dengan membandingkan antara sekolah negeri dengan sekolah swasta; 2) Menganalisis hubungan antara karakteristik siswa dengan tingkat kenakalan siswa di sekolah; 3) Menganalisis hubungan antara karakteristik keluarga dengan sosialisasi dalam keluarga; 4) Menganalisis hubungan antara karakteristik lingkungan sekolah; 5) Menganalisis hubungan antara sosialisasi dalam keluarga dan sosialisasi dalam lingkungan sekolah dengan tingkat kenakalan siswa di sekolah; 6) Mengetahui upaya yang dilakukan oleh keluarga, sekolah dan masyarakat untuk menanggulangi kenakalan siswa di sekolah.
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Judul: Hubungan antara karakteristik individu, pengasuhan oleh orang tua dan keterkaitan teman sebaya dengan tingkat kenakalan pelajar pada sekolah menengah kejuruan teknik industri di Kota Bogor
Abstrak: Secara umum penelitian ini bertujuan untuk mempelajari hubungan antara karakteristik individu, pengasuhan dan keterikatan teman sebaya dengan tingkat kenakalan pelajar. Secara khusus tujuan penelitian ini adalah 1) Mengetahui karakteristik pribadi contoh yang meliputi umur, jenis kelamin, kebiasaan sosial dan hobi, penghargaan diri (self esteem) dan karakter, 2) Mengetahui karakteristik keluarga contoh yang meliputi umur orang tua, pendidikan orang tua, besar keluarga, pekerjaan orang tua dan pendapatan keluarga, 3) Mengetahui gaya pengasuhan orang tua terhadap contoh, 4) Mengetahui karakteristik lingkungan teman sebaya yang meliputi kebiasaan pribadi teman dan keterikatan dengan kelompok teman 5) Mengetahui jenis kenakalan remaja 6) Mengetahui hubungan antara variabel penelitian7) Mengetahui faktor-faktor yang mempengaruhi kenakalan pelajar.
Keyword: kenakalan pelajar

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable