Judul: Regeneration of Adventitious Shoots in In Vitro Culture of Kitolod (Hippobroma longiflora (L.) G.Don)
Abstrak: Kitolod (Hippobroma longiflora (L.) G.Don) merupakan tumbuhan liar yang banyak dimanfaatkan oleh masyarakat sebagai obat tradisional. Bila pemanfaatan tanaman ini semakin berkembang, dikhawatirkan akan terjadi kekurangan ketersediaan sumber benih. Penelitian ini bertujuan mendapatkan teknik dan kondisi kultur terbaik dalam perbanyakan tanaman obat kitolod secara in vitro agar tersedia bibit yang dapat digunakan oleh masyarakat secara luas. Metode penelitian menggunakan rancangan acak lengkap (RAL) dengan dua faktor perlakuan dan 10 ulangan untuk semua percobaan kecuali pengakaran tunas adventif. Daun dan petiol kitolod digunakan sebagai bahan eksplan. Eksplan daun pada perlakuan BAP 1 mg/L + NAA 0,1 mg/L memberikan jumlah tunas adventif per eksplan paling tinggi yaitu sampai 49 tunas, namun perlakuan BAP 2 mg/L + NAA 0,1 mg/L lebih efektif dalam inisiasi dan perbanyakan tunas kitolod. Tunas-tunas pada BAP 2 mg/L + NAA 0,1 mg/L juga mampu menghasilkan tunas tertinggi (2,62±0,69 cm) pada media pembesaran dan memberikan 75% keberhasilan dalam tahap aklimatisasi. Media pengakaran terbaik adalah media MS yang ditambah NAA 0,5-1,0 mg/L., Kitolod (Hippobroma longiflora (L.) G. Don) is a wild plant that widely used by the community as a traditional medicine. If this plant is utilized more intensively, there may be a shortage of the plant due to the lack of the seed sources. This study aimed to obtain the best techniques and culture conditions for in vitro propagation of kitolod to provide a large number of planting materials for the community. The experiments were arranged using a completely randomized design (CRD) with two treatment factors and 10 replications for all experiments except shoot rooting. Leaves and petioles were used as explant sources. Leaf explants in 1 mg/L BAP + 0,1 mg/L NAA treatment produced the highest number of adventitious shoots per explant, up to 49 shoots, but 2 mg/L BAP + 0,1 mg/L NAA was more effective for shoot initiation and multiplication. The latter medium was also able to produce the tallest shoots (2,62±0,69 cm) and presented 75% of successful rate over the acclimatization period. The best rooting medium was MS medium added with 0,5-1,0 mg/L NAA.
Keyword: organogenesis, planting materials, tissue culture, traditional medicine

Judul: Penggunaan Beberapa Jenis Sitokinin Terhadap Multiplikasi Tunas dan Pertumbuhan Binahong (Anredera cordifolia [Ten.] Steenis) secara In Vitro
Abstrak: Biological diversity in Indonesia occupied third place in the world that using to fulfill needs of basic necessities and supply of medicines. Utilization of medicinal plant in domestic region become larger in a row with improvement of people awareness to consumed natural medicines. Binahong (Anredera cordifolia [Ten.] Steenis) is one of medicinal plant that have a potention to expand as medicine basic commodity. It also useful to treat any diseases and motivated some researchers to examine their bioactive substances. This examination also need research about multiplication using tissue culture technique. Application various of single cytokinin in different concentration is expected able to influenced shoot multiplication and growth of binahong to keep this plant preservation. The research consist of two experiments that first experiment is monitoring effect of sterilization treatment to contamination level, browning and death. Whereas second experiment is monitoring about the effect media treatment in various of cytokinin (AdSO4, BAP, kinetin and thidiazuron) with different concentration (0,50; 1,00; 1,50; and 2,00) mg/l to shoot multiplication and growth of binahong. In second experiment compiled using RAL statistic method with 10 repetations. Parameters are shoot amount (adventif and lateral shoot), height increment, leaf amount, root amount and callus process. In sterilization treatment shows addition of 70 % alcohol during 3 minutes gived highest success percentage (92,76 %). Lowest success percentage happend in control sterilization treatment (46,99 %). Shoot multiplication and growth of binahong shows in 1 MST. This research produced two various of shoots which is adventif shoot ang lateral shoot. Average great amount of shoots produced by 1,50 mg/l kinetin treatment (2,10 adventif shoot) and 1,50 mg/l BAP (3,90 lateral shoot). Control media not produced both of shoots. MS media with addition 0,50 mg/l kinetin showed average height increment and the best amount of leaves are 4,33 cm and 4,70 sheet. In every treatment shows root formation with greatest number produced by control media (8,80 roots). Callus formed in great part of explants that planted in treatment media except in AdSO4 (0,50; 1,00; and 2,00) mg/l and control media. Conclusion of this research is addition various of cytokinin with different concentration in MS media have obvious influental to the parameters.
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Judul: Transisi Fasa Materi Hadronik ke Materi Kuark pada Inti Bintang Neutron.
Abstrak: Bintang neutron terdiri dari beberapa lapisan yang disusun oleh materi nuklir yang berbeda dengan kerapatan yang semakin tinggi ketika mendekati pusat bintang. Inti luar bintang neutron disusun oleh materi hadronik (nukleon, lepton, dan hyperon), sedangkan inti dalam bintang neutron disusun oleh kuark-kuark bebas (up, down, strange) yang saling berinteraksi. Tujuan penelitian ini adalah mempelajari bagaimana transisi dari inti luar ke inti dalam bintang neutron dengan menggunakan pendekatan RMF (Relativistic Mean Field) untuk materi hadronik dan CIDDM (Confined Isospin Density Dependent Mass) untuk materi kuark. Tekanan transisi diperoleh ketika potensial kimia barion materi hadronik dan materi kuark bernilai sama. Nilai tekanan transisi dengan parameter SU6-TM1 sekitar 87 MeV/fm3 dan diperoleh massa maksimum bintang neutron yaitu 1.92M dengan jari-jari sekitar 8.62 km. Nilai tekanan transisi dengan parameter SU6-G2 sekitar 53 MeV/fm3 dan massa maksimum bintang neutron yaitu 1.88M dengan jari-jari sekitar 8.16 km. Jenis transisi fasa adalah orde satu ditandai dengan diskontinuitas dalam kerapatan energi (adanya mixed phase). Parameter-parameter pada model nuklir yang digunakan menyebabkan perbedaan kekakuan persamaan keadaan (EOS) dari materi hadronik dan materi kuark sehingga nilai tekanan transisi dari fasa hadronik ke fasa kuark juga berbeda.
Keyword: bintang neutron, persamaan keadaan, transisi fasa