Judul: Pengaruh Pengetahuan, Nilai Agama, dan Kelompok Acuan terhadap Perilaku Pembelian Makanan Cake and Bakery belum Bersertifikat Halal MUI.
Abstrak: Perilaku pembelian adalah cara seseorang dalam mempertimbangkan, memilih dan membeli makanan untuk konsumsi, terutama cake and bakery. Penelitian ini bertujuan untuk menganalisis pengaruh pengetahuan, nilai agama, dan kelompok acuan untuk membeli cake and bakery yang belum tersertifikasi halal MUI. Desain penelitian ini adalah cross sectional study dengan metode survei. Penelitian ini terbatas pada periode pembelian Juli sampai dengan Desember 2016. Data dikumpulkan secara cluster random sampling dengan kuesioner yang diisi oleh 92 responden siswa SMA Tri Sukses. Data dianalisis dengan menggunakan analisis deskriptif, korelasi, dan regresi linier berganda. Hasil uji korelasi menunjukkan bahwa kelompok acuan memiliki kaitan dengan perilaku pembelian cake and bakery belum tersertifikasi halal MUI. Pada hasil uji regresi linier berganda menunjukkan bahwa perilaku pembelian dipengaruhi oleh kelompok acuan. Hasil analisis regresi ini menjelaskan 20,6 persen variabel penelitian mempengaruhi perilaku pembelian makanan cake and bakery yang belum bersertifikat halal MUI. Uang yang siswa gunakan dalam satu kali pembelian berkisar antara Rp10 000 hingga Rp50 000.
Keyword: pengetahuan, nilai agama, kelompok acuan, perilaku pembelian

Judul: Pengaruh Pengetahuan dan Kesadaran Konsumen terhadap Perilaku Pembelian Pangan Halal
Abstrak: Indonesia merupakan sebuah negara dengan mayoritas populasi penduduk muslim yang terus meningkat. Hal ini menyebabkan semakin meningkatnya kebutuhan akan pangan halal. Tujuan dari penelitian ini adalah: 1) mengidentifikasi tingkat pengetahuan, kesadaran, dan perilaku pembelian pangan halal; dan 2) menganalisis pengaruh pengetahuan dan kesadaran terhadap perilaku pembelian pangan halal. Lokasi penelitian terdapat di Kelurahan Pasir Jaya dan Kelurahan Kedung Badak, Kota Bogor. Penelitian ini menggunakan metode survey dengan melibatkan 250 ibu rumah tangga yang dipilih secara proportional random sampling. Data dianalisis menggunakan regresi linier berganda dan analisis faktor. Hasil menunjukkan bahwa tingkat pengetahuan konsumen baik dan tingkat kesadaran konsumen sedang, sedangkan perilaku pembelian pangan halal rendah. Semakin tinggi tingkat pendidikan, kesadaran dan pengeluaran akan meningkatkan perilaku pembelian pangan halal.
Keyword: kesadaran, muslim, pangan halal, pengetahuan, perilaku pembelian

Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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