Judul: Kajian Dimensi Bangunan Spillway
Abstrak: Bangunan spillway merupakan bangunan hidrolika dari suatu bendungan yang berfungsi pada saat keadaan darurat (emergency), yaitu keadaan muka air pada saat volume tampungan banjir maksimum. Penelitian ini bertujuan untuk melakukan kaji ulang bangunan spillway pada Bendungan Randugunting berdasarkan kajian terhadap desain existing bangunan spillway, debit rencana bangunan spillway, kedalaman aliran kritis, dan profil muka air bangunan spillway. Tipe bangunan spillway yang direncanakan adalah tipe side spillway dengan tipe mercu Ogee. Hasil analisis luas catchment area Sungai Banyuasin adalah 17.39 km2 dengan panjang sungai 8.52 km. Nilai curah hujan rencana dengan periode ulang 1000 tahun adalah sebesar 180.40 mm. Hasil analisis debit rencana metode rasional sebesar 124.07 m3/det, sedangkan dengan metode HSS Nakayasu distribusi hujan 2 jam sebesar 207.40 m3 /det. Kedalaman aliran kritis di atas mercu bendung bangunan spillway diperkirakan setinggi 1.39 m. Elevasi muka air pada saat banjir maksimum adalah 95.66 mdpl di atas mercu bendung spillway, 88.78 - 90.11 mdpl di segmen saluran samping, 88.23 - 88.78 mdpl di segmen saluran transisi, 61.34 - 88.62 mdpl di segmen saluran peluncur, dan 66.28 mdpl di segmen akhir peredam energi., Spillway is a hydraulics building of a dam that functions during an emergency, when the water level of the maximum flood storage volume. This study aims to review the spillway building on the Randugunting Dam based on a study of the existing design of the spillway building, the design discharge of the spillway building, the critical flow depth, and the water level profile of the spillway building. The planned type of spillway building is a side spillway type with an Ogee crest type. The analysis of Banyuasin River catchment area are 17.39 km2 with the river length of 8.52 km. The result of the planned rainfall with a return period of 1000 years is 180.40 mm. The result of discharge with rational method is 124.07 m3/s, while with the Nakayasu HSS method the 2-hour rain distribution is 207.40 m3/s. The critical depth above the weir crest is estimated to be 1.39 m. The maximum water level during flooding is 95.66 masl above the spillway weir crest, 88.78 - 90.11 masl in the side channel segment, 88.23 - 88.78 masl in the transition channel segment, 61.34 - 88.62 masl in the drop channel segment, and 66.28 masl in the stilling basin segment.
Keyword: Critical flow depth, design discharge, water surface profile

Judul: Kaji Ulang Bangunan Spillway pada Proyek Bendungan Ciawi - Cipayung, Kabupaten Bogor.
Abstrak: Pengendalian banjir pada Bendungan Ciawi - Cipayung dilakukan dengan pengoperasian bangunan bottom outlet dan bangunan pelimpah. Penelitian ini bertujuan untuk melakukan kaji ulang bangunan Spillway (pelimpah) pada proyek Bendungan Ciawi - Cipayung, Kabupaten Bogor berdasarkan hasil analisis dari kondisi bangunan pelimpah dan bangunan bottom outlet, koefisien limpasan, waktu konsentrasi, intensitas curah hujan, debit rencana bangunan pelimpah, debit rencana bangunan bottom outlet, kedalaman aliran kritis dan pengaturan debit outflow. Hasil analisis koefisien limpasan (c) sebesar 0.32 dengan waktu konsentrasi (tc) sebesar 1.91 jam, sehingga intensitas curah hujan untuk periode ulang 25 tahun sebesar 51.56 mm/jam dan periode ulang 1000 tahun sebesar 86.42 mm/jam. Debit rencana bangunan bottom outlet dan bangunan pelimpah dihitung dengan menggunakan metode Nakayasu, yaitu sebesar 387.4 m3/det dan 670.8 m3/det. Kedalaman aliran kritis (hc) pada bangunan pelimpah dalam kondisi debit rencana sedalam 2.28 m, sedangkan dalam kondisi debit sungai maksimum sedalam 3.87 m. Pengaturan debit outflow dalam kondisi normal adalah sebesar 316.3 m3/det sesuai dengan kapasitas bangunan bottom outlet.
Keyword: bangunan bottom outlet, bangunan pelimpah, debit outflow, debit rencana, kedalaman aliran kritis

Judul: Using cytochrome β (Cyt β) gene for species identification of dog, cat, and tiger to ensure food authenticity and drugs
Abstrak: Adulteration food products from animal still happen until now. Species identification method development in food and medicine derived from animal was needed to prevent falsification information. Molecular genetic improvement allows the detection is carried out at the level of DNA, so the results more accurate, although processed. Research on several meat have been conducted by several researchers using DNA mitochondrial DNA (mtDNA). Using mtDNA because it has high copy number in a cell, compared with nuclear DNA. In this case where the amount of extracted DNA is very small or degraded. Gene used as marker specific species is cytochrome β (cyt β) because it has varied sequences. The objective of this research was to study species authentication (i.e. dog, cat, and tiger) to ensure animal origin in product using cyt β gene specific marker. Total DNA samples for each species which identified are three samples were consisting blood (cat), cooked meat (dog), feces (tiger), and as a comparison used blood sample from several animal (i.e. goat, chicken, cattle, sheep, horse, rat) and pork. Reverse primer specific for dog, cat, and tiger was designed using MEGA 5 software. DNA extraction and fragment amplification was conducted using phenol-chloroform and multiplex PCR method, respectively. Multiplex PCR is a variant of PCR which many primers were used together for amplification of multiple region. This research showed that different length of amplification for the species tested (dog, cat, and tiger) were 523, 331, 319 bp, respectively; and for the comparison species (goat, chicken, cattle, sheep, pig, horse, and rat) were 157, 227, 274, 331, 398, 439, and 603 bp, respectively. Species specificity also indicated by high reverse primer homology percentage. Specific fragment amplification on genome pool with closed fragment length should be separate to avoid band overlapped between species. Taxonomy hierarchy (i.e. dog, cat, and tiger) could be distinguished to the species level.
Keyword: cat, cytochrome β gene, dog, multiplex PCR, tiger