Judul: Kesesuaian Lahan Pinus merkusii Jungh et de Vriese pada Areal Bekas Tectona grandis Linn. f. (Studi Kasus di RPH Wanareja KPH Banyumas Barat)
Abstrak: West Banyumas Forest Management Unit (WBFMU) is one of the forest management units in working area of Perum Perhutani Unit I Central Java, which has a Corporate Class (CC) of pine trees. Recently, the WBFMU intend to expand the acreage of pine stand in order to increase pine resin production. To achieve this purpose, WBFMU use the former logged over area of teak to be planted by pine. But, the problem raises because the planted pine seedlings showing unappropiate growth. So that, this research was aimed to determine the suitability of pine and limiting factors affecting pine growth in teak stands area at WBFMU. The results show that the suitability of land in the area of the former teak stands in plot 22 B is suitable (S) and plot 25 C is not suitable (N) for the development of P. merkusii according to bonita.
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Judul: Pendugaan potensi simpanan karbon pada tegakan Pinus (Pinus merkusii Jungh. et de Vriese) di KPH Cianjur, Perum Perhutani Unit III Jawa Barat dan Banten
Abstrak: Global warming is one of the major environmental issues that currently concern to the various parties. Because of global warming, increasing in average temperature of earth's sea and land caused by industrial activity and reduction of forest cover due to deforestation, especially lately. Based on these evolving issues and considering the importance of the role of forests in absorbing carbon from the air and store it in forest biomass, it is necessary a lot of research that could encourage the continued development of carbon in biomass. One important aspect of research is to know the potential of carbon stored in the stands of pine. The experiment was conducted in KPH Cianjur, Perum Perhutani Unit III West Java and Banten in April 2010 to May 2010. Materials used in this research were planting stands of pine in 1994 on square 27A RPH West Hanjawar BKPH South Sukanagara and planted stands of Pine year 1995 on square 48I RPH East Hanjawar BKPH North Sukanagara in KPH Cianjur, Perum Perhutani Unit III West Java and Banten. The tools used in this study include scales, digital scales, ovens, machete, newsprint, chalk, plastic bags, plastic rope, stationery, tally sheets, calculator, tape measure, camera, and software SAS. In the area of stands are made each 5 plots with a size of 20 m x 20 m to measure the trees and also made small plots measuring 2 m x 2 m by 4 pieces located at each corner for analysis and retrieval of vegetation and litter. Results obtained from these data are processed through the approach of biomass which is then converted to carbon savings in ton/ha. To know the factors that influence the use of carbon deposits by using ANOVA analysis and Duncan Multiple Range Test (DMRT). The results showed that the potential of carbon storage on plots planted in 1995 is 77,1580 ton/ha greater than the potential carbon storage on plots planted in 1994 which only amounted to 64,6372 ton/ha. The test results using ANOVA statistical analysis showed the value of R-Sq = 96,01% and significant level 5%, there is a difference between the carbon potential on one of the object variabel (tree, manure, below plant) which is found on the stands of pine planted in 1994 and stands pine planting in 1995, where the variable is a tree. There needs to be further research on the estimation of carbon stock potential using a different method of harvesting method with the same kind of pine stands.
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Judul: Excretory/secretory antigen of ascaridia galli L3 stage to trigger yolk immunoglobulin in laying hens
Abstrak: Ascaridiosis caused by the nematode Ascaridia galli. A study was carried out to characterize excretory/secretory antigen of A. galli from larval stage to trigger anti-ascaridiosis yolk immunoglobulin in laying hens. A. galli L3 were recovered from intestines of 100 heads chickens 7 days after oesophagus inoculation with 6000 L2. L3 recovered in this manner were cultured (5 – 10 ml-1) in flasks containing rosswell park memorial institute (RPMI) 1640 media, pH 6.8, without phenol red. Cultures were incubated at 370C in 5% CO2 and culture fluid was collected after 3 days in culture. To prepare excretory/secretory antigen, culture medium were concentrated with vivaspin 30.000 MWCO, and antigen protein concentration were counted as described in Bradford method. The molecular weight of excretory/secretory antigen was determined with sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). Active immunizations were applied intra muscularly of chickens with an initial dose of 80 μg. The immunizations were repeated three times with dose of each 60 μg with an interval of one week. The first immunizations were excretory/secretory antigen mixed with fruend adjuvant complete and subsequently mixed with freund adjuvant incomplete. The chicken’s eggs were collected from 49 day after immunizations. Anti-ascaridiosis yolk immunoglobulin (IgY) was extracted from egg yolks by means of ammonium sulphate and purified using fast performance liquid chromatography (FPLC). The purity of anti-ascaridiosis IgY was determined by Bradford method (λ = 280 nm). Antibody was detected by agar gel precipitation test (AGPT) and enzyme linked immunosorbant assay (ELISA). Antigen were detected with immunohistochemistry. Chickens were challanged with dose of 1000 L2 A. galli. Passive immunizations were applied ten times with dose of each 0,875 mg IgY with an interval of one day intra orally. IgY anti-ascaridiosis was tested against A. galli survival in vitro.
Keyword: Unggas, Isolasi dan karakterisasi antigen, Purifikasi imunoglobulin yolk, Pengukuran antibodi ayam petelur, Metode Imunohistokimia, Ascaridiosis