Judul: Reliability Study of Amyloid Kit for Detection of Alzheimer's Disease
Abstrak: Penyakit Alzheimer adalah penyakit neurodegeneratif yang merusak otak secara bertahap dan merupakan bagian dari demensia. Penyakit demensia Alzheimer disebabkan oleh berbagai faktor sehingga sampai saat ini belum diketahui dengan pasti terapi yang tepat. Pembuatan kit sebagai alat diagnostik untuk menunjang pengembangan penapisan terhadap penyakit Alzheimer merupakan upaya strategis yang dapat dilakukan. Penelitian ini bertujuan untuk melakukan kajian reliabilitas kit deteksi amiloid yang dikembangkan menjadi uji alternatif penapisan penyakit Alzheimer dibandingkan dengan kit komersial. Penapisan berbasis pendeteksian dengan teknik Enzyme-Linked Immunosorbent Assay (ELISA) dapat bekerja secara efektif dan akurat dalam mengoptimalkan bahan antibodi spesifik atau antibodi monoklonal terhadap peptida Aβ42. Berdasarkan hasil dan interpretasi dari nilai batas deteksi, koefisien inter dan intra- assay, kit ELISA yang dikembangkan secara in house memiliki kemampuan deteksi Aβ42 yang menyamai Kit ELISA komersial yang telah tersedia (MyBioSource MBS288507). Kata kunci: Aβ42, ELISA, penapisan, penyakit Alzheimer, reliabilitas kit, Alzheimer’s Disease is a progressive neurodegenerative disease that damages the brain and is part of dementia. Alzheimer’s dementia is caused by various factors that until now the proper therapy is uncertain. Kit production as a diagnostic tool to support the development of screening for Alzheimer’s disease is a strategic effort. Screening based on detection with Enzyme-Linked Immunosorbent Assay (ELISA) can work effectively and accurately to optimize a specific antibody material or monoclonal antibody to the Aβ42 peptide. This study aimed to research the reliability of the amyloid detection kit which was developed as an alternative screening test for Alzheimer’s disease compared to commercially available kits. Based on the results and interpretation of limits of detection, inter and intra-assay coefficients, the in-house ELISA kit has comparable Aβ42 detection to the commercially available ELISA kit (MyBioSource MBS288507). Keywords: Aβ42, Alzheimer’s disease, ELISA, reliability kit, screening
Keyword: ELISA, Reliabilitas kit, Macaca fascicularis, Penyakit Alzheimer, Penapisan, Aβ42

Judul: Detection of Beta-Amyloid 42 as a Marker of Alzheimer's Disease Using the IPB In-house ELISA Kit
Abstrak: Protein amiloid beta 42 (Aß42) merupakan penanda biologis (biomarker) penyakit Alzheimer yang dapat dideteksi menggunakan kit ELISA. Penelitian ini bertujuan untuk mengetahui performa kit ELISA Aß42 inhouse di level serum dibandingkan dengan kit komersial. Penelitian ini dimaksudkan bermanfaat dalam mendeteksi dini penyakit Alzheimer dan pengembangan penelitian menggunakan kit ELISA inhouse IPB-PSSP. Penelitian ini menggunakan 18 sampel serum manusia yang dibagi menjadi 3 kelompok usia: kelompok 1 (20 – 30 tahun), kelompok 2 (30 – 40 tahun), dan kelompok 3 (> 40 tahun). Nilai OD antar kelompok dan antar kit dibandingkan rata-rata serta standar deviasinya. Hasil pengujian kit ELISA Aß42 inhouse memiliki performa yang mendekati kit ELISA komersial., Amyloid beta 42 (Aß42) protein is a biological marker (biomarker) of Alzheimer's disease that can be detected using an ELISA kit. This study aims to determine the performance of the in-house Aß42 ELISA kit at the serum level compared to the commercial kit. This research is intended to be beneficial for the early detection of Alzheimer's disease and for the development of research using the IPB-PSSP in-house ELISA kit. This study used 18 human serum samples divided into 3 age groups: group 1 (20-30 years), group 2 (30-40 years), and group 3 (> 40 years). The OD values between groups and between kits were compared using the average and standard deviation. The results of testing the in-house Aß42 ELISA kit show performance that is close to the commercial ELISA kit.
Keyword: alzheimer, ELISA

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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