Judul: Cloning and Structure Prediction of C-Type Lectin 19 (CTL-19) from Silkworm (Bombyx mori L.)
Abstrak: Bombyx mori L. is a highly significant insect due to its silk production affects several industries. Unfortunately, B. mori farming has recently experienced mortality issues caused by pathogenic infectious diseases. Despite the extensive study of innate immunity of B. mori, information regarding the molecular functions and mode of actions of C-type lectin 19 (CTL-19) in the innate immunity of B. mori remains limited. Therefore, this study aimed to predict the structure of the B. mori CTL-19 also clone and produce the recombinant protein to better understand its role in the silkworm's innate immunity. The open reading frame (ORF) of the CTL-19 gene (945bp) was successfully amplified and cloned to pGEM®-T Easy Vector. The mature protein consists of 294 amino acids with 2 CRDs, 9 conserved cysteine, and two specific carbohydrate binding motifs. Protein threading demonstrated to be superior in order to predict the ligand binding site in comparison to homology modelling. The predicted molecular weight is 33.26 kDa, with pI value of 5.70. Furthermore, molecular docking analysis reveal that BmCTL-19 have ability to bind to various carbohydrate residues. The BmCTL-19 gene will be sub clone into an appropriate expression vector and produce purified recombinant protein to evaluate its properties.
Keyword: Innate immunity, Carbohydrate Binding Motif, Homology modelling, Protein Threading, Molecular docking

Judul: Karakterisasi Gen Ser2 pada Ulat Sutera Domestik (Bombyx mori)
Abstrak: Insect of the family Bombycidae is a beneficial insect because it produces cocoon as a source of natural silk fibers. Silk fibers from Bombyx mori is produced in the silk gland at the stage of last instar larvae before the formation of the cocoon. The main components of silk are fibroin and serisin. Ser2 gene dominates process of fiber formation when the caterpillar is on the growth stage. The study of genes characteristic serisin 2 in B. mori is still limited. The objective of this study was to analyze characteristic of Ser2 gene in silkworm B. mori originated from Candiroto. Middle silk gland (MSG) of the silkworm B. mori was extracted for the RNA analysis using the RNeasy Mini Kit (Qiagen). Complementary DNA (cDNA) was synthesized from RNA using Revertaid Transcriptase (Thermo Science Inc). It was then amplified using three pairs of the primers, i.e two pairs of housekeeping genes and one pair of Ser2 genes. The result of Ser2 gene amplification was sequenced to obtain composition of nucleotide bases. Sampels BC8 and BC10 were successfully sequenced and then aligned using Molecular Evolutionary Genetic Analysis software version 4 (MEGA 4.0). To obtain kindship percentage, those sequenced samples were compared with other individuals acquired from gene bank using Basic Local Alignment Search Tool-nucleotide ( BLAST n) program. The results was that B. mori BC8 and BC10 having similarity as high as 100% with Bombyx mori serisin 2 (Ser2) transcript variant 2, mRNA (Genebank: NM001172817.1), Bombyx mori serisin 2 (Ser2) transcript variant 1, mRNA (Genebank: NM001172816.1) dan Bombyx mori serisin 2 precursor (Ser2) gene, complete cds alternatively spliced (Genebank: GQ381286.1) .
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Judul: Perencanan Lanskap Sunda Kalapa, Jakarta Utara Sebagai Kawasan W isata Sejarah
Abstrak: Perkembangan kota Jakarta dari waktu ke waktu sangatlah cepat, sehingga menyebabkan kota-kota tua banyak yang tergusur untuk dijadikan daerah baru yang lebih modern. Apabila hal tersebut tidak ditanggulangi secara dini maka keberadaan Kota Lama Jakarta akan hilang. Hal inilah yang melatar belakang untuk dilakukan tindakan perlindungan dan revitalisasi kawasan bersejarah Sunda Kalapa.
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