Judul: Mapping of Road Pavement Conditions on Jalan Raya
Cikampak Cicadas to Jalan Segog Pamijahan
Abstrak: Jalan memiliki peran yang penting dalam menunjang kelancaran aktivitas masyarakat. Oleh karena itu, penelitian ini bertujuan mengidentifikasi jenis dan tingkat kerusakan dari perkerasan jalan dan drainase serta menentukan rekomendasi penanganan untuk perkerasan jalan dengan metode Bina Marga. Hasil dari penelitian menunjukan jenis kerusakan dengan luas permukaan terbesar adalah retak memanjang dengan luas total 582,44 m2, dan retak lateral adalah kerusakan dengan luas permukaan terkecil, yaitu 6,27 m2. Tingkat kerusakan cukup tinggi dialami oleh 18 segmen jalan, yang ditandai dengan perolehan nilai kondisi jalan 7. Rekomendasi berupa pemeliharaan rutin, pemeliharaan berkala dan penambahan secara berturut-turut pada drainase sisi timur 27, 18, dan 10 segmen sedangkan pada drainase sisi barat 35, 14, dan 6 segmen. Rekomendasi penanganan pada jalan diperoleh hasil 10 segmen jalan memerlukan pemeliharaan rutin, 26 segmen jalan memerlukan pemeliharaan berkala dan 19 segmen jalan memerlukan peningkatan dengan biaya penanganan yang diperlukan secara berturut-turut Rp 39.857.105, Rp Rp 302.529.219 dan Rp 1.719.020.425., Roads have an important role in supporting the smooth running of community activities. Therefore, this research aims to identify the type and level of damage to road pavement and drainage and determine treatment recommendations for road pavement using the Bina Marga method. The results of the research show that the type of damage with the largest surface area is longitudinal cracks with a total area of 582.44 m2, and lateral cracks are damage with the smallest surface area, namely 6.27 m2. A fairly high level of damage was experienced by 18 road segments, which was indicated by obtaining a road condition score of 7. Recommendations in the form of routine maintenance, periodic maintenance and successive additions on the east side drainage were 27, 18 and 10 segments while on the west side drainage it was 35, 14, and 6 segments. Recommendations for road handling were obtained as a result of 10 road segments requiring routine maintenance, 26 road segments requiring periodic maintenance and 19 road segments requiring improvement with the required handling costs being Rp 39.857.105, Rp 302.529.219 and Rp 1.719.020.425.
Keyword: drainase, kerusakan jalan, pemeliharaan, jalan

Judul: Penilaian Kondisi Jalan dengan Metode Surface Distress Index dan Pavement Condition Index serta Rekomendasi Penanganan
Abstrak: Jalan raya sebagai sarana transportasi memiliki berbagai fungsi dan manfaat yang hanya akan berjalan efektif ketika jalan berada pada kondisi baik. Oleh karena itu, penelitian ini bertujuan menilai kondisi jalan dengan metode SDI dan PCI serta merekomendasikan penanganan kerusakan. Kerusakan yang terdapat pada Jalan Letjen Ibrahim Adjie terdapat kerusakan retak kulit buaya, keriting, ambles, tambalan, alur, jembul, retak tepi, bahu turun, retak memanjang dan lubang sedangkan pada jalan Lingkar Laladon terdapat kerusakan retak kulit buaya, ambles, tambalan, pelepasan butir, retak tepi, bahu turun dan lubang. Nilai kondisi Jalan Letjen Ibrahim Adjie metode SDI diperoleh nilai 85 (sedang) dan metode PCI diperoleh nilai 75,3 (satisfactory). Nilai kondisi Jalan Lingkar Laladon metode SDI diperoleh nilai 235 (rusak berat) dan metode PCI diperoleh nilai 16,9 (serious). Rekomendasi penanganan untuk Jalan Letjen Ibrahim Adjie adalah pemeliharaan berkala berupa perbaikan standar P4, P5, P6 dan U6. Rekomendasi penanganan untuk Jalan Lingkar Laladon adalah rekonstruksi menggunakan perkerasan beton dengan tebal LFA kelas A 125 mm dan pelat beton 175 mm. Rencana anggaran biaya perbaikan standar sebesar Rp33.826.000 dan rekonstruksi sebesar Rp1.549.957.000
Keyword: kerusakan jalan, pavement condition index, surface distress index

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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