Judul: Analysis of Perceptions Analysis and Factors Affecting the Implementation of Green Consumption Behavior in Little Talk Coffee Shop Consumers
Abstrak: Meningkatnya model bisnis indsutri pangan di Indonesia yang perlu didisrupsi dengan menghadirkan inovasi yang lebih ramah lingkungan seperti substitusi bahan kemasan yang tidak ramah lingkungan. Konsumen bijak sebelum melakukan pembelian suatu produk akan memikirkan terlebih dahulu dampak dari produk yang dikonsumsinya. Penelitian ini bertujuan untuk mengdentifikasi persepsi konsumen (generasi Z) terhadap produk hijau yang disajikan di kedai kopi Little Talk dan menganalisis pengaruh pengetahuan lingkungan, sikap lingkungan dan produk hijau terhadap penerapan perilaku konsumsi hijau ketika mengunjungi kedai kopi Little Talk. Metode analisis data yang digunakan dalam penelitian ini adalah analisis deskriptif kuantitatif dengan skala likert dan Structural Equation Modeling - Partial Least Square (SEM-PLS) dengan bantuan software SmartPLS4. Hasil penelitian menunjukkan konsumen (generasi Z) setuju dengan produk hijau yang disajikan oleh kedai kopi Little Talk. Berdasarkan hasil pengujian hipotesis sikap lingkungan berpengaruh signifikan terhadap produk hijau dan perilaku konsumsi hijau, sedangkan pengetahuan lingkungan tidak berpengaruh signifikan terhadap sikap lingkungan, produk hijau, dan perilaku konsumsi hijau. Dengan demikian perlu peningkatan pengetahuan konsumen (generasi Z) terhadap lingkungan untuk mendorong peningkatan perilaku konsumsi hijau., The increasing business model of the food industry in Indonesia needs to be disrupted by presenting more environmentally friendly innovations such as substituting packaging materials that are not environmentally friendly. Wise consumers before purchasing a product will first think about the impact of the products they consume. This study aims to identify consumer perceptions (generation Z) of green products served at Little Talk coffee shops and analyze the influence of environmental knowledge, environmental attitudes and green products on the application of green consumption behavior when visiting Little Talk coffee shops. The data analysis method used in this research is quantitative descriptive analysis with a Likert scale and Structural Equation Modeling - Partial Least Square (SEM-PLS) with the help of SmartPLS4 software. The results showed that consumers (generation Z) agree with the green products served by Little Talk coffee shops. Based on the results of hypothesis testing, environmental attitudes have a significant effect on green products and green consumption behavior, while environmental knowledge has no significant effect on environmental attitudes, green products, and green consumption behavior. Thus, it is necessary to increase consumer (generation Z) knowledge of the environment to encourage increased green consumption behavior.
Keyword: green product, environmental knowledge, environmental attitude, Likert scale, SEM-PLS

Judul: Green Consumption Behavior of Indonesian Young Consumers: The  Role of Knowledge, Responsibilities, and Attitudes towards the Environment
Abstrak: Konsumsi merupakan salah satu penyebab masalah lingkungan yang harus segera diatasi. Konsumen sebagai pelaku utama kegiatan konsumsi memiliki tanggung jawab utama dalam aksi penyelamatan lingkungan. Tujuan penelitian ini adalah untuk menganalisis pengaruh pengetahuan lingkungan, tanggung jawab lingkungan, dan sikap lingkungan terhadap perilaku konsumsi hijau di kalangan konsumen muda Indonesia. Penelitian ini menggunakan mix methods dengan metode pengumpulan data berupa survei online¸ wawancara mendalam, dan observasi. Sebanyak 328 responden terlibat sebagai contoh dalam penelitian menggunakan teknik voluntary sampling. Analisis data dilakukan secara deskriptif dan SEM menggunakan bantuan aplikasi SPSS 25.0 dan LISREL 8.80. Hasil penelitian ini menunjukkan pengaruh yang positif signifikan pengetahuan lingkungan terhadap tanggung jawab lingkungan, sikap lingkungan, dan perilaku konsumsi hijau. Tanggung jawab lingkungan berpengaruh positif signifikan terhadap sikap lingkungan. Sikap lingkungan berpengaruh positif signifikan perilaku konsumsi hijau. Pengetahuan, tanggung jawab lingkungan, dan perilaku konsumsi hijau konsumen muda Indonesia berada pada kategori sedang sedangkan sikap lingkungan berada pada kategori tinggi. Dengan demikian perlu peningkatan pengetahuan dan tanggung jawab konsumen terhadap lingkungan untuk mendorong peningkatan perilaku konsumsi hijau., Consumption is one of the causes of environmental problems that must be addressed immediately. Consumers as the main actors in consumption activities have the main responsibility in environmental saving actions. The purpose of this study was to analyze the effect of environmental knowledge, environmental responsibility, and environmental attitudes on green consumption behavior among young Indonesian consumers. This study uses mixed methods with data collection methods in the form of online surveys, in-depth interviews, and observations. A total of 328 respondents were involved as a sample in the study using voluntary sampling technique. Data analysis was carried out descriptively and SEM using SPSS 25.0 and LISREL 8.80. The results of this study indicate a significant positive effect of environmental knowledge on environmental responsibility, environmental attitudes, and green consumption behavior. Environmental responsibility has a significant positive effect on environmental attitudes. Environmental attitudes have a significant positive effect on green consumption behavior. Knowledge, environmental responsibility, and green consumption behavior of young Indonesian consumers are in the medium category while environmental attitudes are in the high category. Thus, it is necessary to increase consumer knowledge and responsibility towards the environment to encourage an increase in green consumption behavior.
Keyword: attitude, environmental responsibility, green consumption behavior, knowledge

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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