Judul: Hubungan Body Image, Kebiasaan Makan, dan Aktivitas Fisik dengan Status Gizi Remaja Putri di SMA Plus Al-Fatimah Bojonegoro
Abstrak: Tujuan penelitian ini adalah untuk menganalisis hubungan antara body image, kebiasaan makan, dan aktivitas fisik dengan status gizi remaja putri di SMA Plus Al-Fatimah Bojonegoro. Desain penelitian ini adalah cross-sectional study. Jumlah sampel yang digunakan dalam penelitian ini sebanyak 130 orang siswi berusia 15-18 tahun. Body image diukur menggunakan Body Shape Questionnaire (BSQ). Hasil penelitian menunjukkan bahwa sebagian besar contoh memiliki status gizi normal (92.3%), sebanyak 50.8% contoh memiliki body image negatif. Kebiasaan makan diukur menggunakan Adolescent Food Habits Checklist (AFHC) yang spesifik mengukur kebiasaan makan sehat, sebagian besar contoh memiliki skor kebiasaan makan sehat yang tergolong kurang baik (60%). Tingkat aktivitas fisik (PAL) contoh tergolong ringan. Berdasarkan uji pearson, terdapat hubungan yang signifikan antara body image dengan status gizi (p<0.05), akan tetapi tidak terdapat hubungan yang signifikan antara kebiasaan makan sehat dan aktivitas fisik dengan status gizi (p>0.05).
Keyword: aktivitas fisik, body image, kebiasaan makan, status gizi

Judul: Study about Body Image, Eating Habit, and Nutritional Status of Female Student at SMAN 1 Ciomas
Abstrak: Penelitian ini bertujuan mengetahui hubungan antara body image, kebiasaan makan, dan status gizi pada siswi di SMAN 1 Ciomas. Desain penelitian yang digunakan adalah cross sectional study dengan subjek berjumlah 65 siswi kelas X dan XI di SMAN 1 Ciomas. Hasil penelitian ini menunjukan bahwa berdasarkan metode Figure Rating Scale (FRS), sebanyak 60% subjek memiliki body image positif. Namun, terdapat 11 dari 16 subjek yang berstatus gizi normal dengan body image negatif yang menganggap bentuk tubuhnya saat ini belum ideal dan menginginkan bentuk tubuh yang lebih langsing. Sedangkan berdasarkan metode Multidimensional Body-Self Relations Questionnaire-Appearance Scale (MBSRQ-AS) sebanyak 67,7% subjek memiliki body image normal dan 16,9% memiliki body image positif. Subjek memiliki kebiasaan makan yang tergolong baik (56,9%). Sebagian besar subjek (78,5%) memiliki status gizi normal berdasarkan IMT/U. Terdapat hubungan yang signifikan antara subskala MBSRQ-AS evaluasi penampilan dengan status gizi (p= 0,021: r= 0,286). Tidak terdapat hubungan yang signifikan antara body image dengan status gizi dan status gizi dengan kebiasaan makan. Kata kunci : body image, kebiasaan makan, remaja perempuan, status gizi, This study aims to determine the relationship between body image, eating habits, and nutritional status of female student at SMAN 1 Ciomas. The design of study was cross sectional study with 65 female students in 1st and 2nd grade at SMAN 1 Ciomas as the subject. The results showed that based on the FRS method, 60,0% of the subjects had positive body image perception. The results of this study indicate that based on the Figure Rating Scale (FRS) method, 60% of the subjects have a positive body image. However, there were 11 out of 16 subjects with a normal nutritional status that have negative body images who consider their current body shape to be not ideal and want a slender body shape. Meanwhile, based on the MBSRQ-AS method, 67,7% of the subject had normal body image perception and 16,9% had positive body image perception. Subjects tend to had healthy eating habits (56,9%). Most of the subjects (78,5%) had normal nutritional status based on BMI for Age. There was a significant correlation between the MBSRQ-AS’s subscale appearance evaluation with nutritional status (p= 0,021: r= 0,286). There was no significant correlation between body image perception with nutritional status and nutritional status with eating habit. Key words: body image, eating habit, female adolescence, nutritional status
Keyword: body image, eating habit, female adolescence, nutritional status

Judul: Isolasi Konstruksi dan Rekombinasi Gen Pengkode Reverse Transcriptase asal Simian Retrovirus-2 (SRV-2)
Abstrak: The availability of reverse transcriptase enzym as a valuable component for Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique has been providing by foreign companies. Therefore, it needs more cost and time to provide it. This study is an early step to produce in house reverse transcriptase rekombinant enzym from SRV-2. The purpose of this study was isolated, constructed, and rekombined SRV-2 reverse transcriptase gene to entry clone and destination vector. Isolation was done by PCR amplification technique using three pairs of primers, which are RT I (3282-3683), RTII (3261-3900), and RTIII (3169-4133). Construction and recombination in this study used Gateway method from Invitrogen. SRV-2 reverse transcriptase gene was isolated in this study with A-549 cell inoculated SRV-2 as source of virus. This gene amplify by PCR technique and resulted specific band for RTI 401 bp, RTII 639 bp, and RTIII 964 bp. Analysis of pENTR/SD/TOPO and pDEST17 indicated SRV-2 reverse transcriptase gene code has already inserted to this plasmid. This result indicated SRV-2 reverse transcriptase gene have isolated, constructed, and recombined to entry clone and destination vector.
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