Judul: Pengaruh Kelekatan Remaja dengan Ayah -Ibu dan Konsep Diri terhadap Perilaku Cyberbullying Remaja
Abstrak: Kemajuan teknologi membuat interaksi remaja melalui media sosial semakin tinggi dan perilaku cyberbullying rentan terjadi. Perilaku cyberbullying berdampak negatif bagi remaja. Penelitian ini bertujuan untuk menganalisis pengaruh kelekatan remaja dengan ayah-ibu dan konsep diri terhadap perilaku cyberbullying remaja. Penelitian ini melibatkan 205 remaja berusia 15-20 tahun dari SMA dan SMK di Kota Bukittinggi. Pemilihan lokasi penelitian dan contoh dilakukan secara purposive sampling, dikumpulkan dengan teknik self-report menggunakan kuesioner online. Hasil Penelitian menunjukkan tidak terdapat perbedaan yang signifikan kelekatan remaja dengan ayah dan ibu, konsep diri dan perilaku cyberbullying antara remaja SMA dan SMK. Pada dimensi cyberstalking terdapat perbedaan signifikan, remaja SMK memiliki perilaku cyberstalking lebih tinggi dari remaja SMA. Hasil uji korelasi menunjukkan semakin tinggi kelekatan remaja dengan ayah-ibu dan konsep diri maka semakin rendah perilaku cyberbullying remaja. Perilaku cyberbullying dipengaruhi secara negatif dan signifikan oleh kelekatan ayah dengan remaja dan konsep diri.
Keyword: cyberbullying, kelekatan remaja dengan ayah, kelekatan remaja dengan ibu, konsep diri, remaja

Judul: Pengaruh Gaya Pengasuhan Ayah, Kelekatan Teman Sebaya, dan Self Esteem terhadap Cyberbullying
Abstrak: Cyberbullying adalah suatu bentuk kejahatan dengan memanfaatkan media elektronik dan internet terhadap orang lain dengan tujuan tertentu. Secara general, penelitian ini bertujuan untuk menganalisis pengaruh gaya pengasuhan ayah, kelekatan teman sebaya dan self esteem terhadap perilaku cyberbullying remaja. Penelitian ini menerapkan desain explanatory research dengan metode survey online. Total responden dalam penelitian ini adalah 180 remaja. Hasil uji korelasi menunjukkan gaya pengasuhan authoritative berhubungan positif signifikan dengan self esteem dan berhubungan negatif signifikan dengan cyberbullying. Gaya pengasuhan authoritarian berhubungan positif signifikan dengan cyberbullying remaja. Secure attachment berhubungan negatif signifikan dengan cyberbullying, sedangkan insecure attachment berhubungan negatif signifikan dengan self esteem dan berhubungan positif signifikan dengan cyberbullying. Self esteem memiliki hubungan negatif signifikan dengan cyberbullying. Hasil uji pengaruh menunjukkan bahwa gaya pengasuhan authoritative berpengaruh negatif signifikan terhadap perilaku cyberbullying, sementara gaya pengasuhan authoritarian berpengaruh positif signifikan terhadap perilaku cyberbullying. Insecure attachment berpengaruh terhadap perilaku cyberbullying.
Keyword: gaya pengasuhan ayah, kelekatan teman sebaya, self esteem, cyberbullying

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable