Judul: Identifikasi Keragaman Gen UTMP Exon 4 Pada Sapi Bali
Abstrak: The bali cattle, one of Indonesian specific animal genetic resource, has many advantage, one of that advantage is high in reproductivity. Uterine Milk Protein (UTMP) gene is expected having an effect on lifes’ productivity related to the characteristic of reproduction and has main function in supporting conception during pregnancy. The aim of this research was to identify the existence of UTMP polymorphisms gene as controller of reproductions characteristic in Bali cattle using the PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). One hundred twenty three blood samples of bali cattle derived from BPTU and BIBD was detected its presence of genetic diversity at UTMP gene using BsrI which restric C|CAGT basic sequences. UTMP gene amplification was performed with temperature of annealing 60 ⁰C. The results of PCR-RFLP analysis indicated that bali cattle have been analysed monomorphic A allele at BPTU and BIBD was 100%. Result of sequence fragment UTMP gene exon 4 was indicate insersi mutation 77 basic until long change sequence it bands 406 base pair.
Keyword: PCR-RFLP, uterine milk protein (UTMP), bali cattle

Judul: Keragaman DNA mikrosatelit lokus HEL9 ILSTS6 dan INRA35 pada Sapi Bali di Pulau Bali dan Nusa Penida
Abstrak: Sapi bali (Bos javanicus) adalah salah satu bangsa sapi asal Indonesia yang merupakan domestikasi dari banteng. Tujuan penelitian ini adalah mengidentifikasi keragaman DNA mikrosatelit pada sapi bali yang berada di Pulau Bali dan Nusa Penida dengan menggunakan lokus (HEL9, ILSTS6, dan INRA35). Sampel yang digunakan sebanyak 95 sampel DNA sapi bali yang diambil dari 48 ekor sapi bali di pulau Bali dan 47 ekor sapi bali di pulau Nusa Penida. Tiga penciri mikrosatelit (HEL9, ILSTS6 dan INRA35) digunakan untuk mengidentifikasi keragaman sapi bali. PCR menggunakan aplikasiGenAlex versi 6.501. Data yang dianalisis berupa jumlah alel (na), alel efektif (ne), frekuensi alel, heterosigositas observasi (Ho), heterosigositas harapan (He), polymorphism information content (PIC), dan uji Amova. Hasil penelitian menunjukkan bahwa rata-rata heterosigositas pada sapi bali yang ada di Pulau Bali lebih tinggi dibandingkan Nusa Penida. Jumlah PIC sapi bali berkisar 0.190-0.666. Ketiga lokus yang diuji terhadap sapi bali di pulau Bali dan Nusa Penida berbeda dari marker, frekuensi alel dan tingkat kekerabatan dengan nilai keragaman sebesar 9%...
Keyword: Bali cattle, Microsatellites, Polymorphism, Polymerase chain reaction, DNA markets, Alel number, Effective allel, Ale, Ho, He, PIC

Judul: Hydroxyapatite-Chitosan Composite Coating on Stainless Steel 316 to Improve Corrosion Resistance
Abstrak: Metals that were used in bone implantation should have good corrosion resistance that were not easily corroded when implanted into the body. Metal corrosion resistance of stainless steel 316 can be enhanced through the metal coating by hydroxyapatite-chitosan composite with composition ratio of hydroxyapatite (gram) and chitosan 3% (mL) 0.2:0, 0.2:0.1, 0.2:0.5, 0.2:1.0, and 0.2:1.5. Besides improving the corrosion resistance of metal, the presence of chitosan also maintain the position of hydroxyapatite on metal surfaces. Corrosion resistance increases with increasing amount of chitosan added and the best composition of hydroxyapatite-chitosan composite was 0.2:1.0.
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