Judul: Model Sorpsi Isotermi Panili Batang dengan Cara Desorpsi
Abstrak: Buah penili merupakan bagian dari tanaman panili yang bernilai ekonomis tinggi. Buah ini memiliki aroma yang khas dan dipergunakan untuk memberi aroma pada makanan, minuman, es krim dan lain-lain. Sebelum diperjual-belikan, buah panili mengalami proses pengolahan yang meliputi pelayuan, pemeraman, pengeringan, sortasi dan pengepakan. Buah panili hasil pengolahan ini disebut juga panili batang.
Keyword: 

Judul: Model sorpsi isotermi jagung dan gabah serta penerapannya dalam penyimpanan
Abstrak: Sekitar 77 model sorpsi isotermi bahan biologis telah diturunkan dan terdapat diberbagai Pustaka. Dalam penerapannya model-model tersebut memiliki kendala, antara lain hanya berlaku pada selang berlengasan nisbi tertentu, pendekatannya dari satu arah, adsorpsi atau desorpsi, dan tidak seluruh model dapat berlaku untuk seluruh bahan pangan. Penyimpanan biji-bijian antara lain bertujuan untuk mempertahankan kadar air bahan pada tingkat yang aman agar mutu biji-bijian tetap tinggi dalam waktu yang lama. Untuk membuat desain lumbung penyimpanan biji-bijian dibutuhkan pengetahuan tentang sifat isotermi bahan yang menunjukkan hubungan antara kadar air keseimbangan bahan dengan kelengasan nisbi udara dalam lumbung. Di Indonesia penelitian mengenai sorpsi isotermi biji- bijian belum banyak diperhatikan dibandingkan negara lain, padahal perubahan kelengasan nisbi dan suhu udara yang akan mempengaruhi kadar air keseimbangan biji-bijian selama penyim- panan perlu diperhatikan. Tujuan penelitian ini adalah (1) menentukan kadar air keseimbangan (Me) untuk komoditi jagung dan gabah pada suhu dan kelengasan nisbi yang berbeda-beda, (2) mencari bentuk model matematik dari grafik sorpsi isotermi komoditi tersebut diatas, dan (3) mempelajari penerapan model sorpsi isotermi tersebut dalam hubungannya dengan penyimpanan komoditi yang bersangkutan. Penelitian ini dilakukan dalam dua tahap. Penelitian tahap pertama adalah penentuan kadar air keseimbangan pada suhu dan kelengasan nisbi. (RH) yang tetap dimana dalam pelaksanaannya menerapkan metoda penentuan kadar air keseimbangan secara statis. Jagung varitas Arjuna dan gabah varitas IR-36 adalah bahan yang digunakan dalam percobaan ini. Perlakuan suhu dibuat empat tingkat, yaitu 20°C, 25°C, 30°C dan 35°C yang diciptakan dalam inkubator yang memiliki simpangan lebih kurang 1°C. Perlakuan kelengasan nisbi udara dibuat tujuh tingkat dengan selang 11.3 persen sampai 79 persen yang diciptakan dari tujuh macan larutan garam jenuh yaitu lithium klorida (RH 33%), potasium karbonat (RH 44 %), sodium bromida (RH 53 %), kupri klorida (RH 68%) dan amonium sulfat (RH 79%). Ketujuh tingkat RH ini mewakili tiga bentuk ikatan air dalam bahan yaitu lapisan tunggal, lapisan janak, dan lapisan kondensasi permukaan.
Keyword: 

Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
Keyword: