Judul: Perencanaan interpretasi trail dari Tanjung Lame-Karangranjang-Cibandawoh-Cikeusik, Taman Nasional Ujung Kulon
Abstrak: Taman Nasional Ujung Kulon merupakan kawasan pelestarian alam terpenting di Indonesia yang ditetapkan sebagai World Heritage site oleh UNESCO. Kawasan ini memiliki luas ± 120,551 Ha, yang terdiri dari kawasan darat 76,214 Ha dan kawasan perairan laut 44,337 Ha. Secara administratif terletak pada Wilayah Kabupaten Daerah Tingkat II Pandeglang Propinsi Banten. Kawasan Taman Nasional Ujung Kulon yang berbatasan dengan Samudera Indonesia adalah pantai selatan Semenanjung Ujung Kulon. Salah satu bagian dari kawasan Semenanjung Ujung Kulon disebelah selatan yaitu jalur TanjungLame – KarangRanjang – Cibandawoh – Cikeusik. Jalur tersebut dilalui oleh pengunjung yang berwisata ke Taman Nasional Ujung Kulon.
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Judul: Perencanaan Interpretasi Kawasan Wisata Alam Batuluhur Taman Nasional Gunung Ciremai Kabupaten Kuningan Jawa Barat
Abstrak: Kawasan wisata alam Batuluhur termasuk zona pemanfaatan Taman Nasional Gunung Ciremai. Kegiatan interpretasi perlu dilaksanakan untuk mengatur perilaku pengunjung. Metode yang digunakan adalah studi pustaka untuk memperoleh data kondisi umum kawasan, observasi lapang untuk data potensi kawasan, dan wawancara untuk data preferensi pengunjung dan kelompok sasaran interpretasi. Kegiatan interpretasi yang telah dilakukan berupa kegiatan pengenalan lebah odeng dan camp fire care. Potensi sumberdaya yang terdapat di kawasan yaitu potensi fisik (danau, curug, dll), geologi (batuan dan tambang kapur), biologi (burung elang ular bido, pohon malaka, dll), serta budaya dan sejarah (sejarah Batuluhur, mitos Situ Tespong, dll). Rencana interpretasi yang disusun memiliki tema, “Batuluhur, dengan beragam sumberdaya bermanfaat yang perlu dilestarikan” yang diwujudkan dalam empat program yaitu: Bird watching pada jalur yang mengitari Embung Air; pengenalan pemanfaatan tumbuhan malaka, cuing, dan melinjo; kegiatan interpretasi seni dan budaya masyarakat Padabeunghar; serta kegiatan trekking dan foto hunting pada jalur yang meliputi Bukit Impian, Embung Air, sampai Curug Tespong.
Keyword: Batuluhur, interpretasi, perencanaan, program, tema

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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