Judul: Analisis Nilai Tambah Kayu Jati: Studi Kasus di KBM-IK II Gresik Perum Perhutani Divisi Regional II Jawa Timur
Abstrak: Tectona grandis yang lebih dikenal dengan nama Jati merupakan salah satu fancy wood yang digemari oleh masyarakat. Jati dapat diolah menjadi kayu gergajian (Rough Sawn Timber) dan kemudian menjadi finished product. Penelitian ini bertujuan untuk menduga nilai tambah dan nilai tegakan jati, serta nilai lahan hutan jati ketika kayu bulat jati diolah di KBM-IK II Gresik Perum Perhutani Divisi Regional II Jawa Timur. Perhitungan nilai tegakan jati didasarkan pada tegakan KPH Banyuwangi Selatan sebagai pemasok bahan baku terbesari di KBM-IK II Gresik pada tahun 2016. Hasil penelitian menunjukan nilai tegakan jati sebesar Rp 5 610 000/𝑚�������3. Ketika kayu bulat diolah menjadi RST Flooring, terjadi nilai tambah sebesar Rp 2 910 000/𝑚�������3. Berdasarkan nilai tegakan tersebut diperoleh nilai lahan sebesar Rp 11 259 298/ha. Tidak terjadi nilai tambah ketika RST Flooring diolah lebih lanjut menjadi Flooring E2E.
Keyword: jati, kayu olahan, nilai lahan, nilai tambah, nilai tegakan

Judul: Analisis Biaya Produksi Kayu Olahan Jati: Studi Kasus di KBM-IK II Gresik Perum Perhutani Divisi Regional II Jawa Timur
Abstrak: Kayu olahan merupakan hasil hutan Indonesia yang memiliki permintaan meningkat di pasar internasional. Perusahaan KBM-IK II Gresik adalah salah satu perusahaan Perum Perhutani yang mengolah kayu jati di Jawa Timur. Penelitian ini bertujuan untuk menganalisis biaya produksi, break even point dan profitabilitas industri pengolahan kayu jati di KBM-IK II Gresik Perum Perhutani Divisi Regional II Jawa Timur untuk meningkatkan produktivitas dan kinerja perusahaan. Hasil penelitian menunjukkan total biaya produksi pada tahun 2016 adalah Rp33.37 milyar. Biaya produksi kayu olahan per unit berkisar Rp15.39 juta/m3 (RST Parquet Block) sampai Rp33.05 juta/m3 (Finished products Decking, Flooring E2E, dan Flooring T&G). Komponen biaya produksi terbesar adalah biaya bahan baku sebesar Rp20.50 milyar (61.43%). Tahapan produksi yang memerlukan biaya terbesar adalah preparing yaitu Rp21.14 milyar (63.37%). Nilai BEP yang diperoleh berkisar dari 40.97% (RST Flooring) sampai 99.24% (Finished Product Parquet Block). Industri pengolahan kayu merupakan usaha yang menguntungkan seperti yang ditunjukkan oleh nilai ROI 52.50%.
Keyword: biaya produksi, break even point, kayu olahan jati, profitabilitas

Judul: DNA Extraction and Characterization of Salmonella‟s Lytic Bacteriophage Genome
Abstrak: Bacteriophages are ubiquitous in nature and are known to proliferate wherever their bacterial hosts exist. Their virion particles can exist independently outside the host, however all phages are need their host to propagate. Several phages are highly specific to host cell surface receptors and any slight changes in structure results in little or no interaction between the phage and its host. Salmonella‟s phage isolated from sewage water where their bacteria exist. Three isolated of phages (phages 15, 19 and 38) were used in this research that concentrated using three types of methods. PEG/ NaCl method was firstly used to concentrate the phages based on their molecular weight to precipitate the phage particles during centrifugation. The second method was Scraping Plaque method. In this method, overlay plaques were scraped and collected in SM buffer to get phage concentrates. Enrichment method was the last method used to concentrate the phages. Phage genome isolation was done using two methods i.e. EDTA/SDS and Proteinase K methods. The first method was done using DNase I to eliminate bacterial DNA followed by SDS/EDTA treatment to release phages DNA and phenol-chloroform-isoamyl alcohol to extract the DNA. DNA was collected by isopropanol extraction. While in Proteinase K method, bacterial DNA was eliminated by DNase I and Proteinase K/SDS was used to degrade phages capsids and ethanol for DNA precipitation. The result showed that PEG/NaCl method resulted the highest phage concentration for phage 15 and phage 19, while scrapping method was the highest for phage 38. The quantity and purity of extracted DNA were low for PEG/NaCl and Scraping methods, while Enrichment method was higher indicated higher purity. Run on agarose gel electrophoresis of extracted DNA confirmed that Enrichment method results were the best while scraping and PEG/NaCl method was appeared smear background. Based on DNA genome size determination, the bacteriphages of Salmonella sp were comparable with other study of Salmonella phages. Phage P15 with genome size of 39,5 kb was close to phage epsilon15. Phage P19 with genome size of 41 kb was close to phage ST160. And phage P38 with genome size of 48,5 kb was close to phage Gifsy-1.
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