Judul: Genetic diversity and mating system in a seed stand of Mindi (Melia azedarach Linn.), Wanayasa, Purwakarta
Abstrak: Mindi merupakan salah satu jenis cepat tumbuh dari famili Meliaceae yang berpotensi untuk dikembangkan di Hutan Rakyat. Upaya peningkatan produktivitas mindi memerlukan informasi dasar terkait keragaman genetik dan sistem perkawinannya. Sifat bunga hermaprodit mindi menimbulkan pertanyaan tentang bagaimana sistem perkawinan yang terjadi dalam populasi mindi. Untuk itu diperlukan analisis keragaman genetik dan tipe perkawinan mindi yang dapat diduga dengan bantuan teknik mikrosatelit. Penelitian ini bertujuan untuk i) mengetahui amplifikasi silang primer mikrosatelit jenis Mahoni (Swietenia spp) dan Mimba (Azadirachta indica) pada jenis Mindi (Melia azedarach Linn.), ii) menduga variasi genetik indukan dan anakan dari tegakan benih Mindi serta iii) mengkarakterisasi parameter sistem perkawinan di tegakan benih Wanayasa (Purwakarta). Sampel yang digunakan terdiri atas 10 pohon induk mindi dan keturunannya masing-masing sebanyak 5 anakan. Primer spesifik Ai-05 dan Ai-34 (jenis mimba) serta SM45 (jenis mahoni) dapat diamplifikasikan pada mindi. Analisis data menggunakan software POPgene versi 1.31 menunjukkan rata-rata keragaman genetik (He) dalam populasi sebesar 0.565. Analisis sistem perkawinan dilakukan dengan bantuan software MLTR (Multilocus Mating System Program) yang menunjukkan nilai outcrossing tm=ts=1,000 berdasarkan metode Expected Maximum (MLTR). Nilai tersebut menunjukkan bahwa mindi di tegakan benih Wanayasa secara umum melakukan perkawinan silang. Informasi mengenai sistem perkawinan ini dapat dimanfaatkan untuk mengetahui cara efektif yang diperlukan untuk tujuan pengelolaan sumber benih.
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Judul: Keragaman Genetik dan Sistem Perkawinan pada Tegakan Benih Mindi (Melia azedarach Linn.) di Wanayasa, Purwakarta
Abstrak: Mindi is a fast growing species belonging to family Meliaceae which is potential to be developed in community forest. Efforts to increase productivity of mindi seed stand require basic knowledge on genetic diversity and mating system. The sexual flower character of mindi is of hermaphrodite type raising a question about the mating system of Mindi. Genetic diversity and mating system characteristics of mindi can be analyzed by microsatellite. This study was aimed to i) cross-amplify microsatellite primers of Mahogany (Swietenia spp) and Neem (Azadirachta indica) in Mindi, ii) estimate genetic variation of mother trees and their offspring and iii) characterize the parameters of mating system of a mindi seed stand. The samples for DNA analysis were 10 mother trees and their respective offspring (n=5). Specific primers Ai-05 and Ai-34 from Neem and SM45 from Mahogany was successfully amplified in Mindi. Data analysis using POPgene version 1.31 showed the average values of genetic diversity of He=0.565 in the seed stand population. Mating system analysis analyzed by multilocus mating system program showed outcrossing values of tm= ts = 1.00 based on the method of expected maximum (EM). These findings indicated that Mindi in the seed stand Wanayasa is predominantly outcrosser. Basic information on the mating system characteristics of Mindi may be used for effective management for increasing the productivity of seed stands.
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Judul: Studi Kasus Liver Neoplasia pada Anjing Shih Tzu
Abstrak: Liver neoplasia is the excessive and uncontrolled replication of hepatocytes that forms nodules visible in ultrasonography. The purpose of this study is to study and discuss the characteristic, diagnosis and treatment of liver neoplasm through the interpretation of ultrasonography and hematology in a Shih Tzu dog. Ultrasonography is conducted with a Chison Q8 ultrasound machine with a 5 to 7 MHz frequency convex probe on lateral recumbency. The blood cells, liver enzymes and nodules sizes were the main parameters in this study. The results in the period of three years from 2021 to 2023 were interpreted and supported with secondary data sources. The Shih Tzu dog is 14 years old in the year 2023 with a body weight of 5.21kg. No obvious clinical signs were observed during previous examinations. However, lethargy, vomiting and anorexia were recorded in its latest examination. Thrombocytosis, granulocytosis, high liver enzymes and hypoglycemia, which are some of the neoplastic clinical manifestations were also presented by this patient. Besides, liver neoplasm, spleen neoplasm and biliary sludge were the sonogram findings of this patient. The liver and spleen neoplasm was observed stable, while the biliary sludge volume fluctuated. The treatment strategy recorded is Ondansetron, Samylin®, Ornipural and Ursodeoxycholic acid.
Keyword: Blood biochemistry profile, Hematology, Liver Neoplasia, Shih Tzu, Ultrasonography