Judul: Respon Fisiologis dan Tingkah Laku Domba Ekor Tipis dengan Waktu Pemberian Pakan yang Berbeda
Abstrak: This study aimed to examine the relationship of feeding time on physiological response and the effect of feeding time on behavior of Javanesse thin tailed sheep. The composition of feed was 70% of concentrate + 30% of grass. The treatment in this study is the feeding time in the morning and evening. Parameters of physiological response that had been observed were heart rate, respiration rate, rectal temperature. Whilethe observed behavior were locomotive, ingestive and resting. This research was designed by completely randomized design with 2 treatments of feeding time with 6 replications for each of it. Data processed by ANOVA. The behavior data was analyzed by student’s test. The result showed that physiological responses of Javanese Thin Tailed Sheep were normal. Locomotive behavior of sheeps by feeding treatment in the morning was higher in the daytime. Ingestive behavior of sheeps by feeding treatment in the morning and afternoon completely reversed. Resting behavior was more frequently in the night in both paramaters.
Keyword: behavior, physiological responses, Javanese thin tailed sheep

Judul: Respon Fisiologis dan Tingkah Laku Domba Garut Jantan dengan Pakan dan Manajemen Pemberian Pakan Berbeda
Abstrak: Improving feeding and managament for sheep it is normally because of improving their performance. Thereby it can be effected to their physiological response and behaviour. This research aimed to compare physiological response and behaviour of garut rams with feed and different management feeding. This research used 16 garut rams (I0) with 2 factors and 4 replicates. First factors is type of feed R1 (40 % grass + 60% concentrate 1) dan R2 (40% mung bean sprout waste + 60% concentrate 2) and the second factors is management feeding in the morning (P) and management feeding in the afternoon (S). Physiological response were observed such as respiration, heart rate, and rectal temperatures. Behaviour were observed such as ingestive behaviour, licking and biteing other goods, resting, eliminasi, vokalisasi, and agonistic. Physiological response and behaviour data were analyzed by analysis of variance (ANOVA). The result shows that heart rate effected by feed. Sheep which is given feeding mung bean sprout waste have a higher value on heart rate (P<0.01). Rectal temperature efected by feed and management. Sheep which is given feeding mung bean sprout waste (P<0.01) and management feeding in the afternoon (P<0.05) have a higher value on rectal temperatures. Management feeding factors in morning of measuring ingestive behaviour have a higher value (P<0.05) but for resting behaviour, management feeding factors in the afternoon have a higher value (P<0.01). The grass have a higher value for eliminative in daylight periode (P<0.05). Both of factors were not significantly different (P>0.05) for measuring all of behaviour garut rams in the allnight periode.
Keyword: physiological response, garut rams

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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