Judul: Analisis keragaman genetik kerbau lokal (Bubalus bubalis) berdasarkan haplotipe DNA mitokondria
Abstrak: Mitochondrial genome (mtDNA) in vertebrate is a circular double stranded DNA molecule. It has a high mutation rate and strictly maternally inherited. This research was conducted to investigate the diversity among local buffalos based on mtDNA’s control region. Samples that were used in this research were native buffalos from 10 Jawa Tengah, 12 Nusa Tenggara Barat, 10 Sumatera Utara and 12 Banten. By in vitro amplification with Polymorphism Chain Reaction method (PCR) using a pair of primer AF22 and AF23, yielded 1145 base pair long. The selected region is a segment spanning the region of end part 3’ cyt-b gene through the partial of the 3’ control region (d-loop). The PCR product was digested with four restriction enzymes, which were AluI (AG↓CT), HaeIII (GG↓CC), HinfI (G↓AnTC) and MspI (C↓CGG). Two haplotypes were detected among 44 samples. The nucleotide diversity among these haplotypes is 0,17%. Haplotype 1 found in all sample locations, while haplotype 2 was only found in one sample from Sumatera Utara. Genetically, the buffalos from Jawa Tengah, Nusa Tenggara Barat and Banten guessed have a same origin heredity (D = 0,000) and they have a near kinship with Sumatera Utara’s buffalo (D= 0,0061).
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Judul: Genetic diversity of microsatellite and cytochrome oxidase subunit I (COI) gene in Indonesian Buffalo
Abstrak: This study aims to determine genetic diversity in Indonesian buffaloes using microsatellite markers and cytochrome oxidase subunit I gene. The sample used in this study were 211 samples of buffaloes (30 samples of swamp buffaloes from Aceh, North Sumatra, Banten, Central Java, West Nusa Tenggara, South Sulawesi, and 19 samples from Riau) and 12 samples of river buffaloes from North Sumatra. Microsatellite markers used were CSSM66, ILSTS61, and ILSTS17 loci. Microsatellite data in term of observed number of alleles (na); effective number of alleles (ne); Observed heterozygosity (Ho); Expected heterozygosity (He); and polymorphism information content (PIC) were analyzed by GenAlEx 6.41 and Cervus 3.1. COI gene sequences were analyzed using MEGA 5.1 software with the Neighbor-joining method and kimura 2 parameter substitution models. This study showed that CSSM66 loci had two alleles and two genotypes. ILSTS61 loci had five alleles and 14 genotypes. ILSTS17 loci had two alleles and three genotypes. Microsatellite genetic distance between populations could separate Indonesian swamp buffalo. The reconstructed phylogeny tree based on COI gene sequences were able to distinguish Subspecies of swamp buffalo and river buffalo. COI gene sequences showed four haplotypes for Indonesian swamp buffalo, namely H1, H2, H3 and H4.
Keyword: genetic diversity, microsatellite, COI, Indonesian buffalo

Judul: Pembuatan Film Tipis Cu2ZnSnS4 sebagai Lapisan Penyerap Cahaya pada Sel Surya Menggunakan Metode Elektrodeposisi
Abstrak: Thin film of Cu2ZnSnS4 (CZTS) is an alternative semiconductor used as light absorber layer on solar cell. CZTS can replace silicon that commonly used as commercial semiconductor. The advantage of using CZTS as absorber layer is due to the abundance of its chemical composer on earth, and its high value of absorption coefficient. The absorber layer was made by using electrodeposition method on ITO glass substrates. The films were deposited at room temperature using potentiostatic mode with a potential of –1.05 V for 30, 45, and 60 minutes. After that, the films were annealed at two different temperatures, 180 ºC and 500 °C, for 45 minutes in N2/H2S atmosphere. Based on X-ray diffraction analysis, the polycrystalline thin film obtained fall into kesterite crystal phase. This type of crystal was obtained when the CZTS was annealed at 500 °C in H2S/N2 atmosphere. The value of optical band gap energy for the CZTS thin film was 2.2 eV with the film thickness about 1.228–6.366 μm. The sample of CZTS with electrodeposition time of 45 minutes and annealing temperature of 500 °C showed the best result and in conformity with absorber layers criteria for solar cells.
Keyword: semiconductor, ITO glass, CZTS thin film, electrodeposition