Judul: Indeks massa tubuh dan gaya hidup kaitannya dengan skor kesehatan dan kemampuan kognitif usia lanjut di Kota Depok
Abstrak: Penelitian ini dilakukan dengan menggunakan desain cross-sectional, terdiri dari 101 contoh usia lanjut di Kota Depok; 45 laki-laki dan 56 perempuan, 32 contoh berumur < 60 tahun dan 69 contoh berumur ≥ 60 tahun. Pengumpulan data dilakukan bulan November 2004 - Maret 2005. Lokasi dipilih secara purposif, dan data BPS diketahui Kota Depok memiliki populasi usia lanjut ≥ 55 tahun cukup besar, sebanyak 6,57% dari total populasi penduduk; kedua kecamatan terpilih memiliki jumlah penduduk yang cukup besar diantara 4 kecamatan lainnya.
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Judul: Growth pattern of children age 0-6 years old in Depok, West Java
Abstrak: Pertumbuhan adalah perubahan dalam ukuran atau massa. Pertumbuhan dan perkembangan dapat terjadi secara bersamaan mulai dari saat pembuahan sampai dewasa. Tujuan penelitian ini adalah untuk mengetahui pola pertumbuhan anak 0-6 tahun di Depok, Jawa Barat. Subjek yang digunakan 455 anak usia 0-6 tahun di Depok terdiri dari 220 perempuan dan 235 laki-laki. Pola pertumbuhan dapat diketahui dengan prosedur antropometri, mengukur berat badan, tinggi badan, tinggi duduk, lingkar lengan atas, dan lingkar kepala. Hasil penelitian menunjukkan bahwa ukuran berat badan, tinggi badan, lingkar kepala, lingkar lengan atas, dan tinggi duduk pada laki-laki lebih besar dari perempuan. Hasil pengukuran di Depok sama dengan hasil pengukuran pada penelitian sebelumnya di Indonesia. Pola pertumbuhan anak usia 0-6 tahun di Depok meningkat pada setiap parameter seiring pertambahan umur.
Keyword: growth, children, anthropometric, depok, nutrition status, socioeconomic status

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable