Judul: Tingkat Stres Dan Strategi Koping Ibu Pada Keluarga Dengan Anak Retardasi Mental
Abstrak: Tujuan umum penelitian ini adalah untuk mengetahui tingkat stres dan strategi koping ibu pada keluarga dengan anak retardasi mental. Secara kbusus penelitian ini bertujuan untuk mengetabui : 1) karakteristik ke[uarga, karakteristik anak, dan karakteristik ibu, 2) dukungan keluarga, kondisi psikososial anak, dan persepsi ibu, 3) hubungan antara karakterlstik keluarga dengan dukungan keluarga, karakteristik anak dengan kondisi psikososial anak, dan karakteristik ibu dengan persepsi ibu, 4) tingkat stres ibu, 5) hubungan antara dukungan keluarga, kondisi psikososial anak, dan persepsi ibu dengan tingkat stres ibn, 6) strategi koping ibu, dan 7) hubungan antara tingkat stres dengan strategi koping stres ibu.
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Judul: Strategi Koping, Tingkat Stres Ibu Rumah Tangga dan pengaruhnya Terhadap Komunikasi Keluarga
Abstrak: Komunikasi keluarga merupakan hal penting dalam menciptakan keakraban, keterbukaan, perhatian antaranggota keluarga terlebih pada masa krisis pandemi Covid-19 ini. Penelitian ini bertujuan untuk mengetahui strategi koping dan tingkat stres terhadap komunikasi keluarga. Penelitian ini melibatkan 50 keluarga berdomisili pedesaan dan 50 berdomisili perkotaan yang memiliki anak usia remaja (minimal usia sekolah menengah pertama) di seluruh Indonesia. Pengambilan contoh penelitian dilakukan secara non probability sampling dengan menggunakan teknik voluntary sampling. Hasil uji korelasi menunjukkan bahwa terdapat hubungan signifikan positif pada strategi koping dan hubungan negatif pada tingkat stres pada komunikasi keluarga. Hasil uji regresi menunjukkan bahwa strategi koping berpengaruh positif signifikan dan tingkat stres berpengaruh negatif signifikan terhadap komunikasi orientasi percakapan. Selain itu, hasil uji regresi menyatakan bahwa strategi koping berpengaruh positif signifikan dan tingkat stres berpengaruh negatif signifikan terhadap komunikasi orientasi konformitas., Family communication is one of the things to create solidarity and attention for family members. Exceedingly during this Covid-19 pandemic. This study aims to determine coping strategies and stress levels on family communication. This study involved 50 families living in rural areas and 50 living in urban areas with teenage children (minimum junior high school age) throughout Indonesia. A sampling of the study by nonprobability sampling using the voluntary sampling technique. Results of correlation test showed coping strategies have a positive significance and stress levels have negative significance in family communication. Results of regression test showed that coping strategy has a significant positive effect and stress levels have a negative significant effect on family communication conversation orientation. In addition, the results of the regression test stated that the coping strategy has a significant positive effect and stress levels have a significant negative effect on family communication conformity orientation.
Keyword: coping strategy, family communication, family with teenagers, housewife, stress level

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable