Judul: Analisis genotipe normal dan abnormal pada klon kelapa sawit (Elaeis guineensis Jacq.) dengan RAPD
Abstrak: The formation of flower and fruit abnormalities in oil palm still unclear. The aim of this study is to analyze the genetic similarities, grouping among normal and abnormal genotypes, and to obtained a specific DNA band for abnormalities by RAPD analysis. Plant materials have been used i.e. MK152, Mk203, MK209, and MK212 (normaVabnormal and male flowers), while clones MK104 and MK176 (normal and abnormal). Amplification of DNA samples have been done with 15 random primers. Genetic similarities and phenogram were analyzed with NTSYS-pc.
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Judul: Abnormality of flowers and fruits in oil palm (Elaeis guineensis Jacq) clones based on of morphology, biochemistry and genomic analysis.
Abstrak: Phenomena of abnormal flowers found was around 0-17% among clones of oil palm, that reduce productivity. Previous research showed that abnormality of flower is related to DNA hipomethylation in calli and leaves tissues of the abnormality plant. The changes of DNA methylation correlates with gen expression in the specific tissue. In the case of oil palm abnormalities occur in flower and fruit tissues. The objectives of this research were to characterize the morphology of flower and fruit, to determine the oil content in relation to malonyl-CoA and asetyl-CoA, to study changes of DNA methylation status and genomic DNA changes related with flower and fruit abnormality. Eighty plants used in this research consisted of three clones, i.e MK152, MK 176, and MK 209. These were obtained from the collection of ”Badan Pengkajian dan Penerapan Teknologi (BPPT) Ciampea”. Characterization of plant morphology was conducted visually, whereas the oil content of mesocarp was analyzed by extracting the oil with soxhlet system, determination of the malonylCoA and acetyl-CoA content by HPLC (High Performance Liquid Chromathography), the quantification of cytosine methylation by performance RP-HPLC, and detection of alteration of the DNA genome was analysed by RAPD technique (Random Amplified Polymorphic DNA). The results showed that abnormalities of female and male flowers as well as fruits were found in tissue culture-derived plants. The rudimentary stamen in the female flower, and stamen of the male flowers were transformed into carpel-like structure. Fruit abnormality was catagorized into five classes, i.e normal, light abnormal (AbR), heavy abnormal (AbB) and two types of severe abnormal, AbSB1 and AbSB2. The content of palm oil in immmature fruit from the three type of fruits (normal, AbR and AbB) were similar. The normal fruit contained around 74.66 -77.26%, AbR 77.47-80.85%, and AbB 73.89-78.62%. The high palm oil content in the normal (MK 152), AbR (MK 209), and AbB (MK 176) were related to higher malonyl-CoA (0.069 0.085, dan 0.068 mg/ml respectively) than the acetyl-CoA (0.036, 0.021 dan 0.023 mg/ml respectively). On the contrary in the severe abnormal fruits, the acetyl-CoA was higher (0.066-0.087 mg/ml) and the malonyl-CoA lower (0.022-0.037 mg/ml). Compared to the normal plant, hypomethylation in leaves tissue was 1.31-4.01% and hypermethylation was 0.69-2.66% in flowers tissue of the abnormal plants. However, changes of DNA methylation status was not found in fruits tissue. Changes of the sequence of DNA genome in the plants within the same clone were detected using five random primers, i.e.OPC-08,OPD-15, W-15, OPC-09 and SC10-19. A band DNA of the normal plant was detected by OPC-08. Changes of the methylation status in leaf and flower tissues was not directly related to the abnormality of the oil palm flowers. The difference of DNA bands were found among plants of the MK 152.
Keyword: oil mesocarp, malonyl-CoA, methylation cytosine, DNA sequence, West Java

Judul: Hydroxyapatite-Chitosan Composite Coating on Stainless Steel 316 to Improve Corrosion Resistance
Abstrak: Metals that were used in bone implantation should have good corrosion resistance that were not easily corroded when implanted into the body. Metal corrosion resistance of stainless steel 316 can be enhanced through the metal coating by hydroxyapatite-chitosan composite with composition ratio of hydroxyapatite (gram) and chitosan 3% (mL) 0.2:0, 0.2:0.1, 0.2:0.5, 0.2:1.0, and 0.2:1.5. Besides improving the corrosion resistance of metal, the presence of chitosan also maintain the position of hydroxyapatite on metal surfaces. Corrosion resistance increases with increasing amount of chitosan added and the best composition of hydroxyapatite-chitosan composite was 0.2:1.0.
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