Judul: Semen Characteristics and Spermatozoa Abnormalities of Local Ram that are Rested for Mating Program for 12 Months
Abstrak: Istirahat kawin dalam waktu tertentu dapat memengaruhi kualitas semen. Penelitian ini bertujuan mempelajari karakteristik semen dan abnormalitas spermatozoa dari domba lokal yang lama diistirahatkan kawin. Penelitian menggunakan 4 domba jantan dewasa yang istirahat kawin selama 12 bulan. Keempat domba diukur lingkar skrotum dan dikarakterisasi semennya. Semen dikoleksi menggunakan vagina buatan dalam 4 kali ulangan. Semen dievaluasi secara makroskopis (volume, konsistensi, warna dan pH) dan mikroskopis (gerakan massa, motilitas progresif, konsentrasi, viabilitas dan abnormalitas). Pengaruh lingkar skrotum (LS) terhadap kualitas semen dan abnormalitas spermatozoa juga dianalisis menggunakan independent sample t test. Hasil penelitian menunjukkan semen memiliki volume 1,08±0,12 mL, warna putih susu, konsistensi encer hingga kental, pH 6,42±0,04, gerakan massa 2,94±0,13, motilitas progresif 79,38±2,17%, konsentrasi 4940±646,49x106/mL, viabilitas 88,15±5,57%, dan abnormalitas spermatozoa total 11,50±3,76% (abnormalitas primer 5,28% dan abnormalitas sekunder 6,23%). Abnormalitas primer yang paling banyak ditemukan adalah undeveloped (3,40%), sedangkan abnormalitas sekundernya adalah simple bent (4,00%). Nilai konsentrasi dan abnormalitas spermatozoa pada penelitian ini jauh lebih tinggi dari yang pernah dilaporkan sebelumnya. Domba dengan LS lebih besar memiliki kualitas semen lebih baik dibandingkan dengan domba dengan LS lebih kecil, terutama berdasarkan variabel motilitas progresif, serta konsentrasi dan abnormalitas spermatozoa. Istirahat kawin dalam waktu lama berpengaruh terhadap kualitasn semen, terutama peningkatan konsentrasi dan abnormalitas spermatozoa., Sexual rest within a certain period of time can affect the quality of semen. The purpose of this study was to study the characteristics of semen and spermatozoa abnormalities from local ram rested ram from the breeding program. The study used 4 rams that had not been used in the mating program for 12 months. The four rams were measured for scrotal circumference and characterized for their semen. Semen was collected by artificial vagina in four repetitions. Semen was evaluated macroscopically (volume, consistency, color and pH) and microscopically (mass movement, progressive motility, concentration, viability, and spermatozoa abnormalities). Also analyzed the effect of scrotal circumference (LS) on semen quality and spermatozoa abnormalities using the independent sample t test. The results showed that the fresh semen had a volume of 1,08±0,12 mL, milky-white color, thin to tick consistency, pH of 6,42±0,04, mass movement of 2,94±0,13, progressive motility of 79,38±2,17%, concentration of 4940±646,49x106 sperm/mL, viability of 88,15±5,57%, and total abnormalities of 11,52±3,78% (primary abnormality 5,28% and secondary abnormality 6,23%). Primary abnormality in the form of undeveloped was found in a high proportion of 7,98±8,20%. Secondary abnormality in the form of simple bent was found in high proportion with an average of 4,00%. The concentration and abnormalities of this study is higher than previously reported. Sexual rest for long time had an effect on semen quality, especially the increased of concentration and total abnormalities of spermatozoa. Scrotal circumference affects progressive motility, concentration, and abnormalities of spermatozoa.
Keyword: local rams, semen, sexual rest, spermatozoa abnormalities

Judul: Kualitas Semen Domba Garut dengan Pemberian Pakan Limbah Tauge dan Indigofera sp pada Pengencer Tris Kuning Telur.
Abstrak: Garut ram is one of good comodity of the livestock, but still traditional management systems. This is a delimiter in garut ram heir superior quality. Semen preservation in several extender can be as choice to maintain genetic superior of garut ram. Semen quality itself affected by the nutrient factors contained in the waste bean sprouts and Indigofera sp such as protein and vitamin E, which is capable of affecting the quality of spermatozoa in garut ram. The research was aimed to study the influence of feeding with 30% waste bean sprouts and 30% Indigofera sp on sperm quality in the tris egg yolk extender. Semen sample were collected from 4 garut male with age around 11 months were two garut ram was fed with limbah tauge and two garut ram was fed with Indigofera sp. Collected semen were diluted in tris egg yolk extender and stored at refrigerator 5 oC until fifth days. Parameter of semen quality was analyzed as follow: for the colour, consistency, pH, volume semen and sperm mass movement were expressed with descriptively analysis. Concentration and cytoplasmic droplet were tested by T-Test analysis. Sperm motility, sperm viability, integrity of sperm plasma membrane and sperm secondary abnormality were analyzed using Completely Randomized Design Factorial (2x5). The first factor was fed (waste bean sprouts and Indigofera sp) and the second factor was storage time (day 1st,2st,3st,4st and 5st). Data was analyzed by Analysis of Variance (ANOVA) and continued with Duncan test to observed the different between treatments. Result of the experiment showed descriptively: colour, concistency and semen pH in all treatment have same quality. Semen volume were collected from garut ram with fed waste bean sprouts better than with fed Indigofera sp, whereas sperm mass movement with fed Indigofera sp better than with fed waste bean sprouts. T-test analyzed showed all treatment were not significant different (P>0,05) to sperm concentration and cytoplasmic droplet. Analysis of Variance showed sperm motility in all treatment were not significant different (P>0,05) whereas in first and second days storage significant better than after third storage (>40%). Such as with sperm viability and integrity of sperm plasma membrane were significant maintaint until fourth storage with percentage (>60% and >50%). Percentage sperm secondary abnormality in treatment with fed Indigofera sp significant better than treatment with fed waste bean sprouts. It can be summarized that all treatment were not influence to sperm quality garut ram 11 years old, but storage time influential to sperm motility, sperm viability, and integrity of sperm plasma membrane. It is suggested to do further research the influence of both feed on adult ram.
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Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4