Judul: Histopathological Study of Macaca fascicularis Lungs Positive for Klebsiella pneumoniae, Histopathological Study of Macaca fascicularis Lungs Positive for Klebsiella pneumoniae
Abstrak: Satwa primata sering digunakan sebagai hewan laboratorium dalam objek penelitian dikarenakan kemiripannya dengan manusia, diketahui proporsi penggunaan satwa primata sebagai hewan coba mencakup kurang dari 1% dari jumlah penggunaan hewan coba secara keseluruhan. Satu di antara jenis satwa primata yang paling sering digunakan adalah monyet ekor panjang (Macaca fascicularis). Klebsiella pneumoniae merupakan jenis bakteri Gram negatif yang bersifat zoonosis dan dapat menyebabkan infeksi pada saluran kemih, nosokomial, rhinitis ozaena, rhinoskleroma, dan bakteremia. Infeksi K. pneumoniae pada satwa primata diketahui pernah dilaporkan di Amerika Serikat pada spesies monyet hijau Afrika. Tujuan dari penelitian ini adalah untuk mengidentifikasi lesio dan tingkat keparahan radang paru-paru akibat infeksi K. pneumoniae pada M. fascicularis melalui pemeriksaan histopatologi. M. fascicularis yang terinfeksi oleh bakteri K. pneumoniae menunjukkan lesio histopatologi pada organ paru-parunya berupa nekrosis supuratif, hemoragi dan kongesti, infiltrasi sel radang, edema, emfisema alveolus, serta pembentukan fibrin dan jaringan fibrosis.
Keyword: histopatologi, lebsiella pneumoniae, Macaca fascicularis, paru-paru

Judul: Spleen Histopathology of Macaca fascicularis with Klebsiella pneumoniae Infection
Abstrak: Satwa primata pada penangkaran rentan terhadap stress dan infeksi agen penyakit, termasuk Klebsiella sp. Keberadaan Klebsiella spp. pada satwa ini telah dikaitkan dengan berbagai kondisi kesehatan serius, termasuk pneumonia, meningitis, peritonitis, sistitis, dan septicemia hingga bisa menimbulkan kematian. Dalam penelitian ini, telah dikonfirmasi bahwa tiga individu Macaca fascicularis (1 jantan, 2 betina) di Pusat Studi Satwa Primata (PSSP) yang mati terinfeksi oleh Klebsiella pneumoniae. Organ limpa diambil untuk dibuat preparat histopatologi dengan pewarnaan hematoksilin eosin dan dievaluasi lesio yang terjadi secara mikroskopis. Hasil patologi anatomi dari limpa menunjukkan adanya lesion nodul putih berukuran 0.5-1.0cm yang berisi massa berwarna kuning, histopatologi menunjukkan adanya koagulasi nekrosis di pulpa putih, infiltrasi sel radang (neutrofil, limfosit, dan sel plasma) dan hemosiderosis di pulpa putih dan pulpa merah, serta perdarahan pada limpa di pulpa merah. Lesio yang ditemukan menunjukkan terjadinya splenitis pada limpa. Keberadaan lesiolesio ini akan menyebabkan disfungsi dari limpa., Primate animals are like humans in both anatomy and behavior and are therefore widely used in biomedical research. In captivity, primate animals are susceptible to stress and infection with disease agents, including Klebsiella sp. The presence of Klebsiella spp. in these animals has been associated with a variety of serious health conditions, including pneumonia, meningitis, peritonitis, cystitis, and septicemia, that can lead to death. In this study, it was confirmed that three Macaca fascicularis individuals (1 male and 2 females) at the Primate Research Center (PSSP) that died were infected by Klebsiella pneumoniae. Spleen organs were taken for histopathological preparations with hematoxylin and eosin staining, and the lesions were evaluated microscopically. Gross anatomy of the spleen showed white nodules measuring 0.5-1.0 cm in diameter, filled with a yellow, solid material. Histopathology results revealed coagulation necrosis in the white pulp, inflammatory cell infiltration (neutrophils, lymphocytes, and plasma cells) along with hemosiderosis in both red and white pulps, as well as hemorrhage in the red pulp. These lesions indicate the presence of splenitis in the spleen, leading to dysfunction in this organ.
Keyword: Klebsiella pneumoniae, Macaca fascicularis, histopathology, spleen

Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4