Judul: Pengklonan kandidat gen resisten terhadap selenium dari isolat bakteri termofilik Geobacillus sp20k
Abstrak: Termophilic bacteria which is resistant to selenium has a specific mechanism enabling it to accumulate and make the toxic selenium becomes nontoxic to the bacteria itself. The mechanism of action is believed because of the existence of a gene that function to confer resistance to Se (Selenium). However, the existence of the gene itself has not been scientifically proven. Therefore it is necessary to do research on thermophilic bacteria to obtain isolate of thermophilic bacteria from hot springs that have gene encoding resistance to selenium (Se) and clone the gene into an E. coli. Isolated genomic DNA of the thermophilic bacteria as well as the plasmid were cut with Eco RI enzyme. Fragments of bacterial DNA was ligated into the vector and then transformed into E. coli cells. Transformants were tested by using blue white colony to show the growth of white colonies indicated the presence of inserted DNA fragment in pGEM3zf while the growth of blue colonies indicated the absence of DNA fragments inserted in pGEM3zf. Molecular weight of the recombinant plasmid was measured using standard curve with obtained assistance line following equation: Y = 19473.807-6294.596X; where X = distance migration and Y = base pairs. Molecular weight of the recombinant plasmid obtained was 14 438 bp.
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Judul: Deteksi Gen Penyandi Resistensi Antibiotik pada Escherichia coli dan Salmonella sp. yang Diisolasi dari Beberapa Peternakan Unggas di Jawa Barat.
Abstrak: Industri perunggasan menggunakan antibiotik sebagai pencegahan, pemacu pertumbuhan dan pengobatan penyakit infeksi bakerial. Oleh karena itu banyak menimbulkan bakteri resisten. Escherichia coli dan Salmonella sp. adalah bakteri penting pada hewan. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi E. coli dan Salmonella sp., menguji sensitivitas dan deteksi gen penyandi resistensi E. coli and Salmonella sp.. Seratus lima sampel swab kloaka unggas digunakan dalam penelitian ini. Isolasi dan identifikasi bakteri menggunakan kaldu tetrationat (khusus Salmonella sp.), kemudian dikultur pada media agar selektif (agar Mac Conkey dan eosin methylene blue untuk E.coli, Salmonella-Shigella untuk Salmonella sp.), pengujian biokimia dan pewarnaan Gram. Isolat bakteri dikonfirmasi dengan amplifikasi gen uspA E. coli dan invA Salmonella sp. menggunakan PCR. Tetrasiklin, oksitetrasiklin, ampisilin, gentamisin, asam nalidiksat, siprofloksasin, enrofloksasin, eritromisin dan kloramfenikol adalah antibiotik yang digunakan dalam penelitian ini. Metode difusi cakram Kirby-Bauer digunakan untuk mendeteksi resistensi bakteri terhadap antibiotik. Hasil uji sensitivitas menunjukkan bahwa E. coli resisten terhadap tetrasiklin (92.3%), oksitetrasiklin (92.3%), ampisilin (100%), gentamisin (61.6%), asam nalidiksat (94.3%), siprofloksasin (69.2%), enrofloksasin (65.4%), eritromisin (96.2%) dan kloramfenikol (0%). Salmonella sp. resisten terhadap tetrasiklin (75%), oksitetrasiklin (75%), ampisilin (75%), gentamisin (12.5%), asam nalidiksat (100%), siprofloksasin (12.5%), enrofloksasin (0%), eritromisin (100%) dan kloramfenikol (0%). Hasil deteksi gen penyandi resistensi antibiotik pada E. coli dan Salmonella sp. berturut-turut yaitu tetA (83.3% dan 33.3%) positif, blaTEM (100% dan 100%) positif, aac(3)-IV (3.1% dan 0%) positif, gyrA (100% dan 100%) positif ermB (6% dan 0%) positif. Hasil penelitian menunjukkan bahwa E. coli dan Salmonella sp. dapat diisolasi dari swab kloaka unggas. Semua isolat E. coli resisten terhadap ampisilin. Semua isolat Salmonella sp. resisten terhadap asam naidiksat dan eritromisin. Gen penyandi resistensi antibiotik yaitu tetA, blaTEM, aac(3)-IV, gyrA dan ermB genes terdeteksi pada isolat E. coli dan gen tetA, blaTEM dan gyrA terdeteksi pada isolat Salmonella sp..
Keyword: antibiotik, resisten, Escherichia coli, gen, Salmonella sp

Judul: The Effects of Variance of Interest rate to Variance of Annuity Future Values
Abstrak: Time value of money is a concept that refers to the difference of the value of money due to time difference. The value of money for present time would not be the same as the value of money in the future. The value of money for present time is called as Present Value (PV) of the money and the value of money for future time is called Future Value (FV) of the money. Determination the value of money, for present and future time, depends on the time and rate of return. The value of money is determined by several factors such as inflation, fluctuation of interest rates, and government policy in securities and taxation. Due to fluctuation of the value of money we need a FV formulation, it is useful for knowing whether an investment can be profitable or not, and this information is useful also in budget analysis. Annuity is defined as a series of payments made for a specific period. The payment, fixed or varied, can be made at the beginning or at the end of the period. Annuity paid at the beginning of the period is called annuity-due and annuity paid at the end of the period is called immediate annuity-certain. The accumulated value of the annuity after few years later is called as a final value or a future value. Burnecki et al. (2003) have discussed about the FV of a fixed-rate annuity in which payments are made according to the arithmetic or geometric series. They have also examined FV for annuities in which payments are made according to arithmetic or geometric series with random rates of interest. This paper examines FV for annuity-due in which payments are made according to arithmetic or geometric series with interest rates as a random variable, which is normal distribution ,  . Future value formula is expressed in recursive form. FV for annuity-due which payments are made according to the arithmetic or geometric series with random rates of interest is calculated by theoretical way and simulation way. The random rates of interest is denoted as k k i  i  , where k i an independent random variable; i is a constant and k  is a random variable which has normal distribution ,  . By using the determined parameters, a sequence of random rates of interest are generated for theoretical calculation. Meanwhile for the simulation, the random rates of interest is generated 1000 times for the simulation. The generated data give mean value, variance, and standard deviation. Moreover, error of mean value, variance, and standard deviation from the theoretical and simulation results are calculated. Error calculation uses Symmetric Mean Absolute Percentage Error (SMAPE). The smaller the value of SMAPE is the more accurate the simulation. The interest rate variances are varied from 0.000036 to 0.0001 to study the relationship between variance of rates of interest and variance of future value. The theoretical and simulation results show that when the variance of random rates of interest increases, then the variance of FV increases linearly. FV of the annuity-due in
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