Judul: Hubungan Antara Keterdedahan pada Media Massa dan Aktivitas Penerangan Jupen Kecamatan di Kabupaten Bogor
Abstrak: Penyebaran dan pemerataan informasi terutama d I pedesaan sangat tergantung pada aktivitas juru penerang kecamatan sebagai ujung tombak penyebaran informasi . Aktivitas juru penerang sangat tergantung pada pengetahuan dan keterampilan , yang' antara lain dapa t diperoleh melalui media massa. Masalah yang timbul sejauh mana hubungan antara keterdedahan pada media massa dan aktif itas penerangan.
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Judul: Program Penerangan Pembangunan Melalui Juru Penerangan (JUPEN) Kecamatan Di Kotamadya Bogor
Abstrak: Pembangunan pada hakekatnya hanya dapat berjalan dengan lancar dan mencapai tujuan secara efektif apabila masyarakat terlibat didalamnya. Keterlibatan masyarakat yang dikehendaki bukanlah sekedar menjadi objek pembangunan belaka, akan tetapi lebih jauh lagi, yakni sebagai subjek. Kehendak ini menuntut adanya partisipasi aktif masyarakat dalam pembangunannya (Kompas, 1984). Namun demikian, perwujudan partisipasi aktif masyarakat dalam kegiatan pembangunan ternyata masih mengalami banyak kendala. Hal ini terutama disebabkan adanya beragam suku, latar belakang sosial, ekonomi dan budaya masyarakat Indonesia. Sehingga memungkinkan terjadinya berbagai perbedaan dalam pemahaman, penghayatan serta pelaksanaan kegiatan pembangunan oleh masyarakat yang pada gilirannya sangat berpengaruh terhadap kehendak masyarakat untuk berpartisipasi aktif...
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Judul: Introduction and expression of tilapia (Oreochromis niloticus) growth hormone gene in catfish (Clarias sp)
Abstrak: The gene transfer technology applied in commercially important aquatic animals is to enhance genetic quality of aquaculture broodstock. This study was conducted to introduce gene encoding growth hormone (GH) in catfish embryos to improve its growth rate. In fish, microinjection was the earliest technique developed to introduce foreign DNA into fertilized eggs. However, the opaquness, stickiness and buoyancy of the embryos, the invisibility of the pronuclei, the togness of the chorion, and the higher mortality of injected eggs make this technique time consuming and requires sophisticated skills. Electropration method is able to produce mass fish transgenic. In this study, we transferred a plasmid containing GH gene of Nile tilapia (tiGH), driven by medaka β-actin promoter (mBP) into catfish using microinjection and electroporation methods, to obtain growth enhanced transgenic fish. The DNA solution (mBP-tiGH) used was 50 μg/ml in sterile distillated water. The parameter observed was survival rate of embryos (SRe), hatching rate (HR), and the percentage of individual carrying mBP-tiGH. Transgenic individual carrying tiGH was identified by PCR ( Polymerase Chain Reaction) method with specific primer for tiGH gene. The analysis of gene expression was conducted by RT-PCR. The results of research from 100 catfish embryos showed that control uninjected treatment was higher SRe and HR of eggs fertilized while the Sre and HR in electroporated-sperm was similar with control (SRe 98.5%; HR 91.2%). Percentage of catfish carrying tiGH gene by microinjection methods was 42.86% (12/28) while by electroporation methods was 87% and 93%. Germ line transmission of the transgene at first generation was 4.0 % - 8.33%. The growth of catfish in founder generation was not different between transgenic and nontransgenic. The growth of transgenic catfish at first generation were up to 7 fold higher compared with nontransgenic fish.
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