Judul: Perception and Participation of Local People in Community Based Forest Plantation Program in Sarolangun Regency, Jambi
Abstrak: The failure of supply timbers to keep pace with industrial demand and conflicts over land property rights in community level have increased the number of forest and land degradation especially in production forest. In an effort to address this problem, the Indonesian Government introduced a new community-based forest plantation program in 2007 called Hutan Tanaman Rakyat (HTR), which allows local communities to have rights and incentives for developing timber plantations on state forest lands. The objectives of this study are to understand local perceptions on HTR regulations, to identify their participation in HTR program, to identify factors influencing their decisions to join the program, and to analyze the correlation between their social economic characteristics and perceptions with their participations in HTR activities. Data and information was gathered from a survey of 81 households from Taman Bandung, Seko Besar and Lamban Sigatal villages in Sarolangun Regency, Jambi. The results of this study showed that the perception level on HTR regulations of local community is in middle level except for regulation about inheritance, rights and obligations which are still in the lower level. Due to relatively new program which started in 2009, the participation level of local community in HTR is still low. This study also showed that ownership of land in HTR area, distance to the area, local perceptions of land allocation, HTR schemes, time period and concession area, inheritance law, rights and obligations, local institution, and socialization activities have significantly influencing people decision in joining HTR. Based on logistic regression models, the perception variables are better in explaining someone’s opportunity to join HTR than social economic variables, but both variables have high correlation with participation level.
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Judul: Evaluasi proses implementasi kebijakan hutan tanaman rakyat di Kabupaten Sarolangun, Jambi
Abstrak: Community forest plantation policy in Sarolangun Regency has poor performance in implementation. Realization of utilization permit timber-forest plantation (IUPHHK) was issued from Sarolangun district government just about 156.44 ha for 18 KK (0.82% from total provisioning area). Low performance and achievements in the implementation of HTR, requires a comprehensive evaluation on implementation of HTR policy.
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Judul: Influenza Specific Immunoglobulin Y Purification from Egg Yolk with Sodium Chloride Precipitation Method
Abstrak: Imunoglobulin Yolk (IgY) yang terdapat dalam kuning telur telah banyak dimanfaatkan untuk imunoterapi dan imunodiagnostik. Salah satu kendala dalam pemurnian IgY dari kuning telur adalah pemisahan Ig dengan lemak, sehingga teknik pemurnian Ig selalu berkembang. Penelitian ini bertujuan untuk melakukan pemurnian IgY dari kuning telur dengan metode pengendapan menggunakan sodium chloride (NaCl) serta melakukan karakterisasi IgY spesifik influenza. Sodium chloride dipilih karena bahan kimia ini relatif murah, ramah lingkungan, dan tidak merusak imunoaktivitas IgY. Kuning telur yang digunakan dalam penelitian ini berasal dari telur ayam layer yang divaksinasi dengan vaksin influenza Flubio® yang berisi virus Influenza A/H3N2, A/H1N1 dan B/Washington. Pemurnian IgY hanya dilakukan pada kuning telur yang mengandung antibodi spesifik influenza. Deteksi keberadaan antibodi spesifik influenza ditentukan dengan uji agar gel presipitasi (AGP) dan metode enzyme linked immunosorbent assay (ELISA). Konsentrasi IgY hasil pemurnian dihitung dengan spektrofotometer dan karakterisasi protein dilakukan dengan metode Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Konsentrasi protein dari hasil pemurnian IgY memiliki nilai rata – rata 50,403 mg/mL. Hasil SDS-PAGE menunjukkan enam pita protein, yaitu struktur IgY utuh (174,63 kDa), Apoprotein-LDL (129,77 kDa), rantai berat IgY (66,54 kDa), Fab ( 47,65 kDa), rantai ringan IgY (21,85 kDa), dan β-livetin (36,74 kDa). IgY hasil pemurnian masih dapat berikatan dengan antigen influenza berdasarkan uji ELISA. Hasil penelitian ini menunjukkan bahwa pemurnian IgY menggunakan metode pengendapan NaCl dapat memurnikan IgY dengan struktur utuh, meskipun masih ada dua protein tidak spesifik hasil pemurnian. IgY yang dapat berikatan dengan antigen influenza mengindikasikan prospek pemanfaatannya untuk pencegahan influenza dan imunodiagnostik., Yolk immunoglobulin (IgY) contained in egg yolk has been widely used for immunotherapy and immunodiagnostics. One of the obstacles in IgY purification from egg yolk is separating Ig from fat, so that IgY purification techniques are always developing. This study aimed to purify IgY from egg yolk using sodium chloride (NaCl) precipitation method and to characterize influenza-specific IgY. Sodium chloride was chosen because this chemical is relatively unexpensive, environmentally friendly, and does not impair IgY immunoactivity. The egg yolks used in this study were collected from layer eggs vaccinated with the Flubio® influenza vaccine, which contains the Influenza A/H3N2, A/H1N1 and B/Washington viruses. IgY purification was only carried out on egg yolks containing influenza specific antibodies. The presence of influenza-specific antibodies was determined by the agar gel precipitation (AGP) test and the enzyme linked immunosorbent assay (ELISA) method. The purified IgY concentration was determined using spectrophotometer, and protein characterization was performed using the Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) method. Protein concentration from purification has a mean value of 50,403 mg/mL . SDS-PAGE result showed six protein bands, namely intact IgY structure (174,63 kDa), Apoprotein-LDL (129,77 kDa), IgY heavy chain (66,54 kDa), Fab (47,65 kDa), IgY light chain (21,85 kDa), and β-livetin (36,74 kDa). The purified IgY still able to bind with influenza antigen based on the ELISA test. The results of this study indicate that purification of IgY using the NaCl precipitation method can purify IgY with intact structure, although there are still two unspecific proteins in the purification results. The resulting IgY can bind to influenza antigens indicating the
Keyword: Immunoglobulin Y, influenza, egg yolk, purification, sodium chloride