Judul: Pengembangan Ekowisata di Kawasan Trianggulasi Taman Nasional Alas Purwo.
Abstrak: Trianggulasi placed in Resort Rowobendo, Alas Purwo National Park. Trianggulasi attraction of ecotourism are diverse, due to the manager could not utilize and manage optimally, it became unmaintained. The main purpose of this research was to formulated development strategy of ecotourism in Trianggulasi based aspects of demand and aspects of supply. The research was conducted with data collection methods such as observation, questionnaires, interviews and literature. Development strategy of ecotourism in Triangulasi work by creating ecotourism products on visitors demand, encouraging parties to immediately operationalize WWAH business space that has been leased, community empowerment, repairing facilities and infrastructure, optimization of marketing and promotion on target market, provide advices to minimize the impact of environmental damage caused by ecotourism, arrangement for visitors and improving the quality of human resources.
Keyword: Alas Purwo national park, ecotourism, development strategy, trianggulasi

Judul: Pengembangan Ekowisata Mangrove Blok Bedul, Resort Grajagan, Taman Nasional Alas Purwo, Provinsi Jawa Timur
Abstrak: Taman Nasional Alas Purwo (TNAP) merupakan kawasan pelestarian dengan formasi vegetasi yang lengkap, salah satu formasinya adalah formasi hutan mangrove yang terletak di mangrove Blok Bedul yang potensial untuk dikembangkan sebagai objek wisata. Penelitian ini bertujuan untuk menyusun strategi pengembangan wisata dengan tahapan identifikasi potensi objek wisata, identifikasi pengelolaan wisata, menentukan motivasi, persepsi, serta preferensi pengunjung, dan menyusun strategi pengembangan wisata. Metode yang digunakan adalah studi literatur, observasi lapang, kuesioner, dan wawancara. Data dianalisis menggunakan skala Likert untuk penilaian motivasi, persepsi, dan preferensi pengunjung, dan strategi pengembangan wisata dianalisis menggunakan SWOT. Hasil penelitian mendapatkan 20 jenis tumbuhan, 5 jenis mamalia, 15 jenis burung air, 1 jenis reptil, 16 jenis pisces, 5 jenis molusca, dan 7 jenis crustaceae. Potensi sosial dan budaya masyarakat setempat adalah kearifan lokal masyarakat dalam mencari ikan, kerang, udang, dan jenis lainnya, serta upacara adat tahunan Petik Laut. Pengelolaan wisata dikelola oleh Balai TNAP, badan pengelola wisata mangrove, dan pemerintah daerah. Kepentingan TNAP sejalan dengan kepentingan desa yaitu, meningkatkan kesejahteraan masyarakatnya tanpa menebang atau mencari kayu di hutan. Motivasi pengunjung tertinggi (4,27) adalah untuk menghilangkan lelah (refreshing), persepsi tertinggi (3,64) pada keadaan di lokasi, dan preferensi tertinggi (4,21) untuk berjelajah dan menikmati keindahan mangrove.
Keyword: mangrove Blok Bedul, pengembangan ekowisata, Taman Nasional Alas Purwo

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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