Judul: Evaluasi Penerapan Konsep Kota Hijau di Kota Bogor
Abstrak: Development of urban areas in Indonesia is very rapid. City is a reflection of local environment condition, citizen behavior and local culture. Activity and development of the city will cause of various environmental problems like the imbalance between urban growth and the improvement of environmental quality. It bring about the city become not liveable. Because of that is very important to create a comfortable city with safety guarantee and be the one with nature by applying the concept of green city. Green city is a concept of a healthy city, considering ecological aspects. This research use Gap Analysis to find out the actual condition of Bogor City and compare it with the ideal condition of green city concept and evalauate the implementation with scoring method. The results of this research showed that Bogor has had a city development plan towards sustainable city, but the implementations still have not been maximized. The implementations not been spread through the region and have not been solve the urban problems of Bogor City.
Keyword: urban landscape, green city, gap analysis

Judul: Evaluasi Penerapan Konsep Kota Hijau di Kota Jakarta
Abstrak: Green city is one of the concept for urban development towards the sustainable city. There are eight ideal indicators into a green city concept in Indonesia; green planning and design, green open space, green building, green waste, green transportation, green water, green energy, and green community. Jakarta is one of the major cities in Indonesia who have some urban problems such as; urbanization, traffic problem, flood, and also waste problem. The purpose of this study is to identify the application of the green city concept using gap analysis and evaluate the application of the concept using scoring method. The results from the formulation showed that Jakarta have related problems to eight green city indicators. The analysis showed that there is a gap between the ideal with actual condition in the green city immpelementation. Gaps caused by the application of green city concept is still in the early stages. The evaluation phase resulted that green transportation has the highest value of 50%, followed by green open space (45.83%), green planning and design (43.75%) green community (25%), green building (25%), green waste (25%), green water (12.50%), and green energy (10%).
Keyword: Jakarta, green city, gap analysis, evaluation

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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