Judul: Analisis Data Complete Bouguer Anomaly Daerah Panas Bumi “A” dengan Metode Trend Surface Analysis Menggunakan Matlab.
Abstrak: Data complete bouguer anomaly (CBA) dalam survei gravitasi merupakan superposisi dari anomali regional dan residual yang terdapat di bawah permukaan daerah penelitian. Anomali regional memiliki frekuensi rendah dan berasosiasi dengan bawah permukaan yang dalam. Sedangkan anomali residual memiliki frekuensi tinggi yang terletak pada permukaan yang dangkal. Fokus eksplorasi geofisika umumnya terletak pada daerah bawah permukaan yang dangkal, karena permukaan dangkal memiliki benda anomali yang heterogen. Analisis polinomial trend surface analysis orde 1 sampai 5 digunakan untuk memisahkan anomali regional dan residual dari data CBA. Hasil pemisahan disajikan dalam bentuk peta kontur, kemudian hasil pemisahan dari setiap orde polinomial diinterpretasikan untuk mendapatkan informasi bawah permukaan. Perhitungan RMS (root mean square) error terhadap anomali residual menunjukan bahwa orde 2 polinomial menghasilkan nilai error yang terkecil.
Keyword: complete bouguer anomaly, trend surface analysis, pemisahan regional-residual

Judul: Aplikasi pengindraan jauh untuk menduga suhu permukaan dan udara di lahan gambut dan mineral dengan menggunakan metode neraca energi: area study Sampit, Kalimantan tengah
Abstrak: Prediction using satellite imagery can be very useful if used to estimate the temperature in different regions with different land cover types. This study aims to analyze the surface and rising air temperatures in peat lands and minerals, with the energy balance approach using satellite image data TM / ETM +. The methodology undertaken is the determination of the study area, initial processing of satellite images that include: data import images, geometric correction, radiometric, land cover classification, extraction of surface temperature values and calculate the components of energy balance to determine the value of air temperature. The results showed that land use change on mineral and peat soil for 15 years resulted in increased surface temperatures and air temperatures. The range of average surface temperature as a whole in 1989 for mineral soil and peat (27-41) oC, In 2004 the surface temperature of the mineral soil (32-40) oC, and peat (29-35) oC, and the average value range of temperatures (25-36) oC. On the other hand detection of surface temperature using thermal bands of satellite imagery, should pay attention to aspects of the use of their land to avoid misinterpretation, because the condition of land cover types were very influential in the increase or decrease the temperature of the sector in a region.
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Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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