Judul: Perkembangan Dan Pertumbuhan Tanaman Gandum (Triticum aestivum) Di Dataran Rendah Mojosari: Perbandingan Antara Pengukuran Dengan Hasil Simulasi
Abstrak: Anoa dataran rendah merupakan salah satu mamalia endemiksulawesi yang statusnya semakin terancam oleh aktivitas perburuan ilegaldan perusakan habitat. Informasi mengenai karakteristik biologidan ekologi satwa ini dalam habitat aslinya masih sangat sedikit dipelajari. Penelitian inibertujuan untukmengetahui aspek bio-ekologi anoa dataran rendah di wilayah hutan pinogu, propinsi gorontalo, selama bulan april-juli 2000
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Judul: Analisis Pertumbuhan dan Perkembangan Gandum (Triticum aestivum L.) Pada Kondisi Cekaman Suhu Tinggi.
Abstrak: Suhu adalah faktor yang sangat berpengaruh dalam pengembangan pertanaman gandum (Triticum aestivum L.). Kondisi cekaman suhu tinggi tidak dapat dihindari dalam perkembangan tanaman gandum di Indonesia yang merupakan tanaman introduksi dari wilayah iklim subtropis dan iklim sedang. Pada percobaan lapang, gandum varietas Nias memiliki tingkat toleransi lebih tinggi dibandingkan varietas Dewata terhadap cekaman suhu udara rata-rata sebesar 29.76 oC. Pemberian perlakuan naungan mampu menurunkan suhu permukaan tanah sebesar 4 oC sampai 9 oC sehingga menurunkan tingkat cekaman pada tanaman gandum. Model simulasi dibuat berdasarkan perbandingan kondisi suhu optimum dengan suhu tinggi, serta kondisi kesuburan dan ketersediaan air optimum. Suhu optimum untuk mendapatkan biomassa biji tertinggi yaitu pada suhu 10 oC. Berdasarkan hasil simulasi pada suhu 30 oC yang mendekati kondisi lapang, umur panen tercapai pada 35 hari dengan biomassa biji sebesar 281.872 Kg/ha, sehingga menunjukkan cekaman suhu tinggi menurunkan biomassa gandum.
Keyword: Gandum, suhu, cekaman, naungan, simulasi

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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