Judul: Kolonisasi dan proliferasi sel testikular ikan nila putih yang ditransplantasikan ke ikan nila hitam triploid
Abstrak: The technology of fish testicular cells transplantation had been established to create a surrogate broodstock. This technology can also be utilized to generate YY-progenies that are useful to produce all-male fish. In this study, as a first step towards all-male tilapia production, white Nile tilapia testicular cells was transplanted into peritoneal cavity of triploid black tilapia larvae. A specific primer was designed to identify colonization and proliferation of transplanted cells in recipient fish using PCR method. Analysis of gonadosomatic index (GSI) was also performed to determine the transplanted cells proliferation in recipient gonad. The result of PCR analysis showed a specific DNA amplification product in the gonad of transplanted fish. The specific DNA donor was detected on the recipient gonad 90 dpt (days post transplantation) and it was not detected on 30 and 60 dpt. The GSI analysis showed that GSI recipient was increased and relatively similar with diploid non-transplanted Nile tilapia and it was higher than triploid non-transplanted Nile tilapia. These results showed that the donor cell could be colonized and proliferated in triploid black tilapia gonad. Thus, it is likely to obtain a broodstock that is able to produce donor-derived gamete in the near future.
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Judul: Transplantasi sel testikular ikan gurame pada ikan nila
Abstrak: One of the constraints in the cultivation of giant gouramy is the lack of fry availability, necessitating the development of fry production engineering technology to address these issues. Engineering technology of fry production through the surrogate broodstock generated by testicular cells transplantation technique has been developed in salmonid. Testicular cells transplantation technology requires a suitable recipient that can accept and support the development of spermatogonia donor cell. This study examined the potential of Nile tilapia as a recipient for donor giant gouramy cells by observing its colonization in the gonads of Nile tilapia. Fish donor of 598,82 grams body weight with Gonado Somatic Index (GSI) of 0,013% and spermatogonial cells percentage of 28,22% was used. Testicular cells in amount of 20.000 cells/0,5 μl PBS were injected into peritoneal cavity of 4-day-old tilapia larvae. The success of transplantation and donor cell colonization were analyzed using PCR method with DNA template that have been extracted from tilapia larvae of 1 day post injection and the gonad of 2 months old fish. PCR was performed using a set of specific primer for the giant gouramy growth hormone gene and tilapia β-actin as an internal control of DNA loading. PCR results showed that the transplanted tilapia has a DNA band in the same size with giant gouramy, and not in untransplanted control. The success rate of transplantation in larvae was 100% (5/5) and the percentage of individual recipients containing donor cells in their gonad was 60% (3/5). This indicates that donor cells from giant gouramy was successfully injected and can be colonized in the gonads of Nile tilapia, so the Nile tilapia potentially be used as recipient for giant gouramy testicular cells transplantation. Further analysis needs to be conducted to observe the ability of spermatogonial cells develop into sperm and eggs in the gonad of tilapia recipient.
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Judul: Iklim Pulau Bali
Abstrak: Curah hujan rata-rata bulanan di Bali berkisar antara 47 mm sampai 327 mm, dengan curah hujan maksimum jatuh pada bulan Januari dan minimum pada bulan Agustus. Total hujan setahun rata-rata di Bali 1901 mm. Pada muim angin barat, curah hujan di utara pegunungan relatif sama dengan di selatan pegunungan, sedangkan pada musim angin timur curah hujan di selatan pegunungan lebih tinggi daripada di utara pegunungan. Isohyet tiap bulan di Bali menunjukkan pengaruh ого- grafis terjadi di bagian timur P. Bali yang mengakibatkan di selatan pegunungan lebih basah daripada di di utara pegunungan selama musim kemarau (monsoon timur). Dari isohyet tersebut dapat juga dilihat bahwa potensi air tertinggi ada di bagian timur P. Bali ini khususnya di bawah kaki G. Agung. Musim angin barat di Bali terjadi pada bulan Desember sampai dengan bulan Maret, dan pada bulan April sampai bulan September merupakan musim angin timur. Musim panca roba terjadi pada bulan Oktober sampai bulan November. ..
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