Judul: Perilaku Penggunaan Pembalut pada Mahasiswi
Abstrak: LUSIANA PUTRI RAHAYU. The Behavior of Sanitary Napkin Utilization on College Students. Under Guidance of LILIK NOOR YULIATI and MEGAWATI SIMANJUNTAK The objective of this research were to identified amount of source information of sanitary napkin; attachment of sample with mother, knowledge about napkin, brand awareness, brand image and the behavior os sanitary utilization; to analyze the relationship of samples’ characteristics and attachment with mothers with sanitary knowledge; to analyze the relationship of top of mind with brand using; and to analyyze factors that affected the suitability of brand using with top of mind. The result showed that 60% percent samples got information from three to five information sources. Half samples were categorized moderate in sanitary napkin knowledge. Seventy percent samples had menstrual from six to seven days per period and 69% samples usually use 11–20 sanitary napkins. During the last three months, most samples used maxi sanitary napkins type and 67% samples used Charm. About sixty three percents samples mentioned that Charm was on top rank in top of mind and the first rank brand recall was Laurier. Correlation between age and education with samples’ knowledge level was positive and significant, only amount of allowance affected significantly the suitability between brand using with top of mind. Keywords: Sanitary Napkin, Utilization Behavior, Brand.
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Judul: Analisis keputusan pembelian dan preferensi konsumen pembalut wanita “Charm” (kasus pada mahasiswi strata 1 Institut Pertanian Bogor)
Abstrak: Modernisasi zaman telah merubah gaya hidup masyarakat kepada pola konsumsi yang cepat, mudah dan praktis. Terdapat perbedaan kebutuhan antara konsumen laki-laki dengan konsumen wanita. Wanita memiliki kebutuhan yang harus dipenuhi oleh suatu produk ketika mengalami fase menstruasi. Produk tersebut biasa disebut dengan pembalut. Di masa kini penggunaan pembalut dianggap cara yang cepat dan praktis untuk memudahkan wanita mengatasi menstruasi dan tetap bisa beraktifitas dengan baik. Hal ini meningkatkan peluang bisnis pada industri pembalut wanita. Akibatnya persaingan dalam industri tersebut semakin pesat. PT Uni-Charm Indonesia merupakan salah satu produsen dari pembalut wanita dengan merek Charm yang berdiri sejak tahun 1997 yang terkena dampak persaingan tersebut. PT Uni-Charm Indonesia dituntut untuk mampu melakukan peningkatan dan pengembangan secara berkesinambungan terhadap produk pembalut wanita Charm.
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Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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