Judul: The Potency of Cissus quadrangula Salisb. Stem Extract on Proliferation and Differentiation of Rats Bone Marrow Mesenchymal Stem Cell into Osteocytes.
Abstrak: Cissus quadrangula Salisb. (CQ) was traditionally used in certain bone diseases treatment, in Aceh. Cissus quadrangula Salisb. contained biochemical compounds such as phytoestrogen, calcium and phosphorous, that were reported can trigered osteogenesis. Mesenchymal stem cells from bone marrow can be differentiated to be several cell types such as osteoblast, osteocyte and adipocyte. This study was conducted to observed potency and optimal dose of CQ in proliferation and differentiation of rats bone marrow mesenchymal stem cell. Mesenchymal stem cells (MSC) were isolated from os femur and os tibia of rats. There were five treatment groups in this study. All treatment groups used modified Dulbecco’s modified eagles’s medium (mDMEM) medium. In the control group was not added CQ extract, while in the other groups were added 0.1 mg/mL (CQ 0.1 mg/mL), 0.3 mg/mL (CQ 0.3 mg/mL), 0.6 mg/mL (CQ 0.6 mg/mL) and 0.9 mg/mL (CQ 0.9 mg/mL) of CQ extract. The level of cell proliferation was analyzed by using population doubling time (PDT) technique. Identification of cell differentiation was done by morphological observation after staining with Alizarin red. Cell differentiation was indicated by the number and diameter of osteoblast and osteocyte. The result showed CQ extract treatments decreased PDT significantly (P<0.01), than that of control. It showed that CQ stem extract increased proliferation of bone marrow mesenchymal stem cell. The number of osteoblast of control treatment group was lowest significantly (P<0.01) than others CQ stem extract treatment groups. The treatment of 0.3 mg/mL CQ stem extract showed the highest number of osteocyte significantly (P<0.01) than others treatment groups. It showed that CQ stem extract increased cell differentiation. The diameter of osteoblast and osteocyte of all treatments groups were not different significantly (P>0.05). It showed that CQ stem extract did not influence on cell diameter. The study concluded that CQ stem extract increased proliferation and differentiation of rats’ bone marrow mesenchymal stem cell into osteoblast and osteocyte. The optimal dose of CQ stem extract was 0.3 mg/mL.
Keyword: Cissus quadrangula Salisb, Mesechymal Stem Cell, Proliferation, Differentiation

Judul: Efek Pemberian Ekstrak Batang Sipatah-patah (Cissus quadrangula Salisb.) terhadap Proliferasi dan Diferensiasi Sel Tulang Tikus secara In Vitro
Abstrak: Research has been conducted on in vitro culture of four weeks old rat (Sprague Dawley) bone cells in DMEM (Dulbecco’s Modified Eagle’s Medium) containing 10% NBCS (Newborne Calf Serum), 10% NEAA (Non Essential Amino Acid), NaHCO3, 1 μl/mL ITS (Insulin Transferrin Selenium), and 50 μg/mL gentamycin (mDMEM), with and without Cissus quadrangula Salisb. extracts (CQ). There are five groups of treatment, consisted of positive control (mDMEM+dexamethasone 10-8 M), negative control (mDMEM), dose 1 (mDMEM+CQ 0.3 mg/mL), dose 2 (mDMEM+CQ 0.6 mg/mL), and dose 3 (mDMEM+CQ 1.2 mg/mL). Culture was done in 5% CO2 incubator at 37 0 C for 7 days. The parameters observed were Population Doubling Time (PDT) done based on calculation using hemocytometer. Osteoblast and osteocyte were stained with Hematoxylin Eosin (HE), and the compotition and diameter of the cell were counted and measured using micrometer. Data were analyzed using statistical ANOVA and Duncan test. The results showed that Cissus quadrangula Salisb. extract at doses 0.3 mg/mL, 0.6 mg/mL, and 1.2 mg/mL increased the bone cells proliferation (P<0.05) and at dose 0.6 mg/mL induced differentiation osteoblast to osteocyte (P<0.05). Differentiation showed by the decreasing diameter of osteoblast thus by increased percentage of osteocyte. In conclusion, the optimal dose of Cissus quadrangula Salisb. extract for in vitro bone cells proliferation and differentiation is 0.6 mg/mL.
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Judul: Mencari Aproksimasi Akar-Akar Polinomial Kompleks Dengan Algoritma Weyl
Abstrak: Mencari akarMakar polinomial dengan menggunakan algoritma Weyt. Algoritma WeyJ merupakan algoritma rekursif yang proses kerjanya dapat diana10gkan dengan algoritma Bisection. Karena algoritma Weyl bergerak dalam bidang kompleks maka memerlukan nilai awal hempa bujur sangkar. Dalam proses penyempitan bujur sangkar tersebut algoritma Weyl memerlukan algorittna Turan untuk menentukan jarak antara suatu titik dengan akar polinomial yang terdekat dengan titik tersebut. Karena apabiJa jarak tersebut temyata lebih hesar dari Yl panjang diagonal bujur sangkar yang diamati maka bujur sangkar tersebut tidak memuat satu pun akar polinomial. Untuk seJanjutnya bujur sangkar tersebut diabaikan. Sebaliknya apabila jarak yang diperoleh lebih keeil dari \6 panjang diagonal bujur sangkar yang diamati, maka bujur sangkar tersebut yang akan diperkeciL
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