Judul: Karakterisasi Koi Herpesvirus (KHV) pada Ikan Mas Cyprinus carpio di Danau Toba.
Abstrak: Koi herpesvirus (KHV) merupakan salah satu faktor pembatas dalam kegiatan budidaya ikan mas dan koi (Cyprinus carpio). Penelitian ini bertujuan untuk mengidentifikasi karakteristik koi herpesvirus (KHV) pada ikan mas yang terdapat di Danau Toba melalui analisis virus dengan uji polimerase chain reaction (PCR), sekuensing DNA, pengamatan gejala klinis dan makroanatomi, serta konfirmasi histopatologi jaringan ikan. Hasil yang didapatkan ialah gen thymidine kinase (TK) KHV yang didapatkan memiliki panjang pita DNA sebesar 411 bp. Gen TK KHV pada sampel memiliki kemiripan susunan basa nitrogen sebesar 98% dengan strain yang sebelumnya teridentifikasi berdasarkan hasil blast di data GeneBank, yakni dengan strain KHV Iran (KX609547.1), strain KHV A2 Indo_7C04NS1 (HM347106.1), strain KHV-Eropa (AB375385), strain KHVJepang (AP008984), dan strain KHV-USA (DQ657948). Gen TK KHV yang diidentifikasi telah mengalami mutasi berupa transisi dan transversi pada susunan basa nitrogennya. Infeksi KHV pada ikan mas yang didapatkan menunjukkan terjadinya gejala klinis, berupa kelainan tingkah laku, kelainan eksternal tubuh, dan kelainan organ internal tubuh ikan. Berdasarkan hasil histopatologi juga didapatkan terjadinya fusi dan pembentukan badan inklusi pada lamella dan gigi-gigi insang sebagai bukti infeksi virus KHV pada ikan.
Keyword: Blast, ikan mas, KHV, mutasi, PCR, sekuensing DNA

Judul: Pengaruh Cekaman Suhu terhadap Penyakit Koi Herpes Virus (KHV) pada Ikan Koi (Cyprinus carpio koi)
Abstrak: Koi fish are susceptible to Koi Herpes Virus Disease (KHVD). The disease is caused by the Koi herpesvirus, and mortality rate up to 80-95% within 2 weeks after infection. Koi herpesvirus is included in the virus that is hexagonal with a diameter of 100 nm. This study aims to determine the effect of temperature stress on the ability of Koi herpesvirus infects KHV free Koi fish and know the nature of transmission. Isolation and identification of Koi herpesvirus originate from the gills of Koi fish organs by the method of polymerase chain reaction (PCR) using PCR-KIT IQ2000TM. In this research, there were 8 fish groups of research, including the 6 fish group of test fish and two control test. The fish were injected with a virus suspension 10% as much as Koi herpesvirus 0.1 ml in intra peritoneal. For 14 days, seven of the first group as a fish's treatment of extreme temperatures at night 18-19 0C and 30-31 0C during the day. The 8th group of fish without control treatment temperature extremes. In a sequence group, the number of fish that were positive of KHVD were 5/5, 7/10, 5/5, 8/10, 0/5, 0/10, 4/5 and 0/5. KHV isolates with codes D143, D144 and M628 extremely virulent KHV isolates and not in the code D139. Stressed fish more susceptible to KHVinfection and KHV-infected fish easily transmit the koi herpesvirus in healthy fish or cohabitation by contamination of water in the aquarium and / or direct contact.
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Judul: Natrium lignosulfonat sebagai agen pendispersi pada mortar
Abstrak: Lignosulphonate, a modification product of lignin waste from pulp and paper industry can be used as water reducer in mortar mixture. This substance can influence the mechanical characteristic of mortar such as diameter of flow, flexural strength, compressive strength, and setting time of cement paste. Lignosulphonate shows it function as dispersant on cement hydration at 3 days that analyzed with x-ray difractometer. Research result show that flow of fresh mortar rise with increase concentration (from weight of cement) of synthetic sodium lignosulphonate (NaLS). It show that mortar homogenity also increasing. The result of flexural strength and compressive strength test use synthetic NaLS 0.1-0.3% from weight of cement show that addition synthetic NaLS 0.2% is the best result because it has the biggest of flexural strenght and compressive strength value. Initial setting time of cement paste increase, about 50% with addition of synthetic NaLS 0.2%. Whereas the final setting time decrease, about 8%. Cement hydration test show increase hydration product peak, Ca(OH)2 dan calcium silicate hydrate (C-S-H).
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