Judul: Analisis metode klasifikasi terhadap hasil klasifikasi kelas spektral data citra spot (Studi kasus daerah Lembang dan sekitarnya)
Abstrak: Adanya kenyataan bahwa hasil klasifikasi citra SPOT sangat tergantung pada perlakuan terhadap datanya, mendorong diadakan penelitian yang intensif tentang perlakuan sebelum klasifikasi, metode pengambilan contoh, metode klasifikasi dan pengaruhnya pada klasifikasi kelas spektral.
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Judul: Pengaruh koreksi radiometrik citra digital terhadap hasil klasifikasi : studi kasus daerah Sukamandi dan sekitarnya
Abstrak: Penelitian ini bertujuan untuk mengetahui pengaruh koreksi radiometrik terhadap hasil klasifikasi tak terbimbing (unsupervised classification) melalui studi per- bandingan antara hasil klasifikasi citra asli dengan citra yang telah dikoreksi dengan saringan spasial. Citra yang digunakan dalam penelitian ini adalah citra daerah Sukamandi dan sekitarnya yang diambil oleh Landsat-5 pada tanggal 6 Juli 1986. Metode klasifikasi yang digunakan adalah klasifikasi tak terbimbing dengan menggunakan algoritma K Rataan dengan konsep jarak Mahalanobis. Sedangkan koreksi radiometrik yang digunakan berupa saringan spasial frekuensi rendah (LPF). Untuk mengetahui pengaruh saringan spasial tersebut dipilih sebanyak tiga daerah klasifikasi yang masing-masing berukuran 25x36 pixel dan empat macam saringan, yaitu f3-9, f5-25, f3-16 dan f3-10. Koreksi radiometrik dengan menggunakan saringan spasial sangat besar pengaruhnya terhadap hasil klasifikasi. Hasil klasifikasi citra hasil penyaringan 36% 66% berubah terhadap hasil klasifikasi citra asli. Perubahan hasil klasifikasi terutama terjadi pada pixel-pixel batas dan kelas-kelas yang jaraknya relatif berdekatan.
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Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.