Judul: Dukungan orang tua, efikasi diri, motivasi akademik, dan prokrastinasi akademik remaja putra dan putri di Wilayah Miskin Perdesaan
Abstrak: Perilaku menunda mengerjakan dan menyelesaikan tugas akademik merupakan permasalahan yang sering terjadi di lingkungan akademik. Penelitian yang menjelaskan secara komprehensif pengaruh faktor internal dan eksternal terhadap prokrastinasi akademik pada remaja di wilayah miskin perdesaan masih jarang dilakukan. Hasil penelitian menunjukkan perbedaan tingkat motivasi intrinsik, motivasi ekstrinsik, amotivasi dan prokrastinasi akademik antara remaja putra dan putri. Remaja putri memiliki motivasi intrinsik dan motivasi ekstrinsik yang lebih tinggi daripada remaja putra. Sementara itu, amotivasi dan prokrastinasi akademik lebih banyak dilakukan oleh remaja putra. Jenis kelamin, dukungan otonomi ibu, efikasi diri, motivasi intrinsik dan motivasi ekstrinsik diketahui berhubungan negatif signifikan dengan prokrastinasi akademik, dan amotivasi berhubungan positif signifikan dengan prokrastinasi akademik. Prokrastinasi akademik dipengaruhi secara negatif oleh jenis kelamin, dukungan otonomi ibu, efikasi diri, dan motivasi intrinsik, serta secara positif oleh motivasi ekstrinsik.
Keyword: dukungan orang tua, efikasi diri, motivasi akademik, prokrastinasi akademik

Judul: Pengaruh Ekspektasi Orang Tua dan Efikasi Diri Akademik terhadap Motivasi Akademik Siswa Sekolah Menengah Atas di Perdesaan
Abstrak: Remaja mengalami berbagai perubahan dalam hidupnya baik fisik dan psikologis. Usia remaja memiliki rentang antara 12-18 tahun dan merupakan usia pelajar. Motivasi akademik merupakan hal yang penting untuk pelajar karena motivasi yang menjadi pendorong bagi pelajar untuk mengeksplorasi segala hal. Penelitian ini bertujuan menganalisis pengaruh karakteristik remaja, karakteristik keluarga, ekspektasi orang tua dan efikasi diri akademik terhadap motivasi akademik siswa sekolah menengah atas di perdesaan. Penelitian ini melibatkan 100 remaja dari tiga sekolah menengah atas di Kecamatan Rumpin. Data dikumpulkan melalui teknik self administered, kemudian diuji menggunakan uji regresi. Hasil penelitian menunjukkan bahwa remaja perempuan memiliki motivasi akademik, efikasi diri akademik dan ekspektasi orang tua yang lebih besar dibandingkan remaja laki-laki. Tidak ada karakteristik yang berpengaruh terhadap motivasi akademik selain jenis kelamin. Ekspektasi orang tua dimensi kedewasaan diri dan efikasi diri akademik memiliki pengaruh positif terhadap motivasi akademik sementara ekspektasi orang tua dimensi prestasi akademik memiliki pengaruh negatif terhadap motivasi akademik.
Keyword: Efikasi diri, ekspektasi, motivasi, perdesaan, remaja, Rumpin

Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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