Judul: Identification of Bioactive Compounds of Moringa oleifera Leaves and Its Potential as Pancreatic Lipase Inhibitor In Silico
Abstrak: Obesitas merupakan penyakit kronis multifaktoral yang berbahaya bagi kesehatan karena memiliki komplikasi terhadap penyakit-penyakit degeneratif sehingga perlu dilakukan langkah pengobatan yang tepat untuk menanggulangi kasus obesitas. Sebuah obat antiobesitas orlistat yang disetujui FDA merupakan inhibitor ireversibel lipase pankreas yang masih memiliki efek samping gastrointestinal. Oleh karena itu, penelitian terhadap senyawa antiobesitas alami diperlukan. Kelor mengandung banyak senyawa alami dan berpotensi sebagai antiobesitas. Penelitian bertujuan mengidentifikasi senyawa aktif dalam ekstrak etanol kelor (Moringa oleifera) dan menentukan senyawa yang berpotensi menginhibisi aktivitas enzim lipase pankreas secara in silico. Metode LC MS/MS-QToF digunakan untuk mengidentifikasi senyawa aktif, dilanjutkan dengan penambatan molekuler secara penapisan virtual dengan aplikasi YASARA Structure. Sebanyak 18 senyawa aktif dari golongan flavonoid, fenolik, dan saponin berhasil diidentifikasi berdasarkan hasil analisis LC MS/MS-QTOF dari ekstrak etanol 70% kelor. Senyawa aktif kelor hasil LC MS dan 72 senyawa hasil studi literatur digunakan sebagi ligan uji dalam penelitian ini. Hasil penelitian menunjukkan bahwa ada sebanyak 32 dari 90 ligan uji berpotensi sebagai inhibitor lipase pankreas. Kaemferol, apigenin, dan luteolin merupakan tiga senyawa terbaik yang berpotensi sebagai inhibitor lipase pankreas., Obesity is a chronic multifactorial disease that is dangerous to health because it has complications for degenerative diseases, Therefore, an appropriate treatment step requires to be taken to overcome obesity cases. An FDA-approved anti-obesity drug, orlistat, is an irreversible inhibitor of pancreatic lipase that still has gastrointestinal side effects. Thus, research on natural anti-obesity compounds is needed. Moringa contains a lot of natural compounds and has potential as anti-obesity. This research aims to identify the bioactive compounds in the ethanolic extract of Moringa oleifera and determine the potential inhibitor of pancreatic lipase in silico. The LC MS/MS-QTOF method was used to identify the bioactive compound, followed by molecular docking based on virtual screening using the YASARA Structure. Out of 18 active compounds were identified based on the flavonoid, phenolic, and saponin results of the LC MS/MS-QTOF from M. oleifera ethanol 70% extract. All identified Moringa bioactive compounds from LC MS and 72 bioactive compounds from literature were used as experimental ligands in this study. The results showed that 32 out of 90 ligands have potential as pancreatic lipase inhibitor. Kaemferol, apigenin, and luteolin are the most potential of pancreatic lipase inhibitor.
Keyword: antiobesitas, inhibitor lipase pankreas, in silico, Moringa oleifera

Judul: Identifikasi Senyawa Aktif Lengkuas (Alpinia galanga) Dengan LC-MS dan Potensinya Sebagai Inhibitor Lipase Pankreas Secara In Silico
Abstrak: Prevalensi penderita obesitas yang terus meningkat setiap tahunnya, serta risiko komplikasi yang ditimbulkan membuat perhatian besar saat ini. Orlistat berefek buruk karena sifatnya yang bekerja dengan menginhibisi enzim lipase pankreas secara ireversibel. Lengkuas banyak ditemukan di Indonesia diketahui dapat berperan sebagai antiobesitas. Penelitian ini bertujuan mengidentifikasi senyawa aktif lengkuas yang berpotensi memiliki aktivitas inhibisi enzim lipase pankreas dengan menggunakan YASARA. Senyawa aktif lengkuas diekstrak menggunakan etanol 70% dan diidentifikasi menggunakan LC-MS/MS QToF serta dilakukan studi pustaka. Sebanyak 8 senyawa berhasil diidentifikasi menggunakan LC-MS/MS QToF dan termasuk golongan polifenol, fenolik, terpen, dan flavonoid, serta terdapat 38 senyawa yang hasil studi pustaka. Terdapat 4 senyawa aktif lengkuas memiliki potensi antiobesitas dengan empat yang terbaik yaitu, galangin, galaganol C, isorhamnetin, dan kampferide. Galangin menjadi senyawa yang paling potensial menjadi kandidat antiobesitas pada tanaman lengkuas., The prevalence of obesity, which continues to increase every year, as well as the risk of complications caused by it, is of great concern today. Orlistat has adverse effects because it works by irreversibly inhibiting the pancreatic lipase. Galangal, found in Indonesia, is known to act as an antiobesity agent. This study aimed to identify active compounds of galangal that have the potential to inhibit pancreatic lipase using YASARA. The active compounds of galangal were extracted using 70% ethanol and identified using LC-MS/MS QToF and literature study. A total of 8 compounds were successfully identified using LC-MS/MS QToF and included polyphenols, phenolics, terpenes, and flavonoids, and there were 38 compounds from literature studies. There are 4 active compounds that have antiobesity potential with the four best ones, namely, galangin, galaganol C, isorhamnetin, and kaempferol. Galangin is the most potential compound to be an antiobesity candidate in galangal plants.
Keyword: Alpinia galanga, pancreatic lipase, antiobesity, molecular docking

Judul: Implementasi Space Time Composite (STC) Data Model Menggunakan Model Data Relasional
Abstrak: The data model is a concept that describes the data stored in the database and the relationship between the data to the user in logic. Development of data models is very important in the design of the database as the basis of the stage of database design to be made. This study aims to apply the concept of Space Time Composite (STC) on the Model Data storage vector data types that can store enough information and describes the process of drafting objects and objects spatiotemporal evolution over time. From the results of the test queries on the data model that have been created, queries in a system are acquired that can help display the information preparation of objects from time to time and know the history of the object polygon and arc that has undergone changes in topology.
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