Judul: Identifikasi Laktoferin pada Kolostrum dan Susu Domba Garut dengan Metode Single Radial Immunodifusi dan SDS-PAGE.
Abstrak: Domba garut merupakan salah satu domba lokal yang belum tereksplorasi secara optimal. Domba garut lebih dikenal sebagai domba pedaging dan domba aduan. Domba garut memiliki potensi sebagai penghasil susu yang dapat memberikan nilai tambah bagi perekonomian masyarakat. Susu domba garut merupakan salah satu sumber laktoferin yang memiliki berbagai manfaat, diantaranya sebagai zat antimikroba. Pemanfaatan susu domba garut sebagai sumber laktoferin diharapkan dapat mengatasi kasus infeksi pencernaan yang tinggi dimasyarakat. Penelitian ini bertujuan untuk mengetahui keberadaan laktoferin pada kolostrum dan susu domba garut dan memperoleh metode yang sesuai untuk mengidentifikasinya. Laktoferin kolostrum dan susu domba garut diidentifikasi dengan metode single radial immunodifusi (SRID) dan SDS-PAGE. Metode SRID dilakukan dengan mendifusikan whey kolostrum dan susu domba garut ke dalam gel yang mengandung anti-laktoferin (Sigma-Aldrich Co.). Metode SDS-PAGE menggunakan konsentrasi gel akrilamida 7,5%. Identifikasi laktoferin dapat dilakukan dengan metode single radial immunodifusi (SRID) maupun SDS-PAGE. Metode SRID tidak mampu mengidentifikasi laktoferin dengan konsentrasi rendah di dalam kolostrum dan susu domba garut. Bobot molekul laktoferin kolostrum dan susu domba garut berdasarkan hasil SDS-PAGE adalah 73.144 Da. Kandungan laktoferin pada kolostrum dan susu domba garut berdasarkan diameter zona presipitin meningkat sampai 48 jam setelah melahirkan dan turun kembali setelah 48 jam setelah melahirkan.
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Judul: Komposisi Kimia dan Konsentrasi Laktoferin dalam Kolostrum Kambing Perah Peranakan Etawah (PE) dan Jawarandu., Ternak perah merupakan hewan ternak yang dapat menghasilkan susu melebihi kebutuhan anaknya. Kambing perah termasuk salah satu ternak perah dengan hasil utama adalah susu. Kambing Peranakan Etawah (PE) dan kambing Jawarandu merupakan bangsa-bangsa kambing perah yang tergolong plasma nutfah Indonesia dan telah dikembangkan potensinya sebagai penghasil kolostrum dan susu yang dapat memberikan nilai tambah bagi perekonomian masyarakat. Kolostrum mengandung banyak senyawa aktif yang penting bagi kesehatan, salah satunya adalah laktoferin. Kolostrum dapat dijadikan sebagai salah satu sumber laktoferin yang memiliki berbagai manfaat, diantaranya adalah sebagai antimikroba. Pemanfaatan kelebihan kolostrum kambing perah diharapkan mampu mengurangi masalah infeksi pencernaan bakterial oleh Escherichia coli yang sering dijumpai khususnya pada anak-anak di Indonesia.
Abstrak: Goats are considered as dairy animals and milk is their main product. Dairy goats colostrums is one of lactoferrin source which has various benefits such as antimicrobial activity. Lactoferrin is one of antibacterial substance that naturally found in milk, especially in colostrums, used to prevent diarrhea in neonatal. Lactoferrin was expected could be used to treat gastrointestinal tract infection bacteriologically cases, that was primarily caused by Escherichia coli which was the major problem in Indonesia. Lactoferrin issues from cows has largely studied, contrairely from dairy goats was very limited. Indonesia has many different of dairy goat breeds that could be interesting to be studied concerning its potenciality as source of lactoferrin. This research has purposed to studied on chemical composition of colostrums and the concentration of lactoferrin. This research also determined the concentration of lactoferrin in colostrums from two different dairy goat breeds, there were Etawah-Grade and Jawarandu breed, using anion exchange chromatography. Experimental design used was Randomized Complete Design with three repetitions and Factorial Randomized Complete Design (2 x 3) with three repetitions. Any significant differences were further tested by using Duncan’s test.
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Judul: Thermal Characteristics and Dynamics of Tropical Cyclone over Western North Pacific Ocean: A Case Study of Typhoon Songda (Chedeng) 2011
Abstrak: The frequent occasion of Tropical Cyclone (TC) over Western North Pacific (WNP) Ocean lies from 10˚N to 26˚N and 121˚E to 170˚E. Sources of TC development come from warm ocean temperature where latent heat is released during condensation processes and subsequently concentrated in the boundary layer. Relative humidity at 850-700 hPa is responsible to produce the most intense storm. Meanwhile, weak vertical wind shear between 850-200 hPa is one of the dynamical factors that lead to the development of TC. Pressure level of 850-200 hPa is chosen to minimize the thermodynamic effect in the lower troposphere. Typhoon Songda (Chedeng in the Philippines) or TSC lasted from May 18th to 30th, 2011 and strengthened into powerful Super Typhoon stage between May 24th and 27th. Long curving track and the exhibition of all sequences of TC life cycle, TSC is then considered as an ideal case of TC. The objective of the study with case of TSC is to investigate the role of thermal characteristics and dynamics on TC intensity. The research is carried out by using Advanced Research of Weather Research and Forecasting model (WRF ARW model) version 3.3. Two domains are employed in the model. The first domain situate from 02˚N to 40˚N and 118˚E to 149˚E and cover spatial resolution () of 33.1 km. The second domain situate from 09˚N to 27˚N and 119˚E to 132˚E with of 11 km. Timestep of the model () is 120 seconds. The model is supported by the following physical parameterization, i.e., Yonsei University Scheme (YSU) in the boundary layer, Kain-Fritsch scheme (KF) in the cumulus, and WRF Single-Moment 3-class scheme in the microphysics. Initial meteorological condition prior to the formation of the cyclone is generated using WRF ARW standard-release model. Sensitivity of the model output is obtained from a selected single-parameter change scenarios, namely, 10 % to 20 % decrease and increase of wind shear initial data at 850-200 hPa, and by 10 % to 20 % decrease of relative humidity initial data at 850-700 hPa. The result showed that the negative correlations between wind shear and intensity are found in wind shear scenarios. Wind shears of 10 ms-1 which lead to strengthening of the wind intensity in the early period of Typhoon development are found in wind shear scenarios. The effect of wind shear changes on wind intensity is statistically significant in the 20 % wind shear scenarios. During mature stage, weak wind intensity occurred when wind shear increase as given in the WRF standard-release and the 20 % decreasing scenario. Different situation is found in the 20 % increasing scenario where wind shear tend to weak and lead to strong wind intensity at this stage. Meanwhile, the reduction of moisture supply at 850-700 hPa resulting from 20 % decreasing relative humidity play a major role in the weakening of wind intensity and reduces mechanical energy of the cycle process as much as 300 J kg-1.
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