Judul: Pengaruh Peran Ayah Terhadap Kecenderungan Merokok Siswa Smp Di Perdesaan Bogor
Abstrak: Permasalahan remaja khususnya kecenderungan merokok sangat memprihatinkan di Indonesia saat ini. Penelitian ini bertujuan untuk menganalisis pengaruh peran ayah terhadap kecenderungan merokok siswa SMP di perdesaan Bogor. Desain penelitian ini menggunakan cross sectional study dengan metode survei secara self-administered menggunakan kuesioner. Penelitian ini melibatkan 60 siswa kelas tujuh dan delapan yang berasal dari keluarga lengkap di dua SMP Kecamatan Pamijahan, Kabupaten Bogor yang dipilih secara proportional random sampling. Hasil uji regresi logistik menunjukkan bahwa lama pendidikan ayah dan gaya pengasuhan otoritatif ayah berpengaruh negatif terhadap kecenderungan merokok siswa. Sementara itu, gaya pengasuhan permisif ayah berpengaruh positif terhadap kecenderungan merokok siswa.
Keyword: gaya pengasuhan, kecenderungan merokok siswa, peran ayah, perdesaan, remaja

Judul: Pengaruh Komunikasi Orangtua-Remaja dan Paparan Media Negatif terhadap Perilaku Pornografi Remaja di Perdesaan Bogor
Abstrak: Perilaku pornografi marak dilakukan oleh remaja di abad ini. Komunikasi antara orangtua dengan remaja tidak seluruhnya berkualitas baik, di sisi lain media negatif sangat mudah diakses oleh remaja. Hal ini berpotensi meningkatkan perilaku pornografi pada remaja. Tujuan dari penelitian ini adalah menganalisis pengaruh komunikasi orangtua dengan remaja dan paparan media negatif terhadap perilaku pornografi remaja di perdesaan Bogor. Desain penelitian ini menggunakan cross sectional study dengan melibatkan 60 remaja usia 15-19 Lahun yang duduk di kelas 10 dan 11 SMK yang dipilih melalui teknik Proportional random sampling. Hasil uji regresi menunjukkan paparan media negatif dan usia ayah berpengaruh positif signifikan terhadap perilaku pornografi remaja di perdesaan Bogor (adjusted R²-0.206; p<0.1)….
Keyword: Adolescents, Negative media exposure, Parent-adolescent communication, Pornography, Rural, Human ecology, Crpss sectional study, Family school partnership, Communication media

Judul: Purification and characterization of thermostable chitinases from bacillus lichemiformis MB-2
Abstrak: Chitinases are of great biotechnological interest and have received remarkable attention. A themophilic bacterium, Bacillus lichenifomis MB-2, from Tompaso hot spring, North Sulawesi Indonesia, secreted thermostable chitinases into culture media. Preliminary study showed that the enzymes occur in multiple forms and were stable at a high temperature and a broad range of pH. The objectives of this research were to purify and characterize thermostable chitinases from B. lichenrformis MB-2. The extracellular chitinases were isolated by successive column chromatographies on Phenyl Sepharose CL4B, DEAE Sephacel, and Superdex G-75. The purified enzymes had molecular weight of 67 (Chi-67) and 102 kDa (Chi-102), estimated by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). Chi-67 and Chi-102 had an endo- and exo- chitinolytic activities, respectively. The optimal temperature of Chi-67 was 70°C, whereas Chi-102 was 60 "C. The optimal pH for both enzymes was 6.0. Chi-67 wets stable below 60°C and over a broad pH range of 4-1 1 for 1 h. In contrast, Chi- 102 was not stable at temperature above 50 "C and only stable at its optimum pH for 1 h. Chi-47 was resistant to denaturation by urea, Tween-20 and Triton-X, but unstable toward organic solvents such as propanol, ethanol, DMSO (dimethyl sdfoxide), and PEG (polyethylene glycol), indicating that hydrophobic interaction of proteins plays an important role in maintaining enzyme activity. Ionic interactions are also important for Chi-67 fold as the activity was reduced by guanidine hydrochloride and NaCl (1 M). Chi- 1 02 was relatively more stable toward various organic solvents than Chi-67. Both enzymes hydrolyzed colloidal chitin, glycol chitin, or glycol chitosan, but were less active to regenerated chitin, fine powder of chitin, or methyl cellulose. The Michaelis constants &) for colloidal chitin, glycol chitin, 4- methylumbelliferyl N', W-diacetylchitobioside [MUF(GICNAC)~], 4- methyiumbelliferyl N', N', M-triacetylchitotrioside [MCTF(G~CNAC)f~o]r Chi-67 were 3.08 mg ml-', 0.3 15 mg ml-', 0.02 pmol d-' and 0.02 pmol dl, respectively. Meanwhile, the K, for colloidal chitin, glycol chitin and MUF(GlcNAc) for Chi-102 were 2.00 mg ml-', 1.32 rng ml-', and 0.03 mM ml-', respectively. The first 13 N-tertninal amino acids of Chi-67 were determined to be SGKNYKIIGYYPS, which is identical to chitinase from B. licImenIfomris PR- 1 (accession no. AA022 144) and B. circuImrs No. 4.1 (accession no. AM23 3 68).
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