Judul: Identifikasi Keragaman Gen Insulin-like Growth Factor-1 pada Kambing Peranakan Etawah di BPTU KDI-HPT Pelaihari Kalimantan Selatan
Abstrak: Etawah grade goats meat productions can be improved through the efforts of livestock genetic enhancement based on the exploration of potential genes that supposedly strong influence in growth trait. Insulin-like growth factor-1 (IGF-1) gene is known to be associated with the milk production and growth traits. The objective of this research was to identify the polymorphism of IGF-1 gene in Etawah grade goats from BPTU KDI-HPT Pelaihari South Kalimantan. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method used to detect the genotype of IGF-1 gene. Polymorphism in 5’-flanking region of IGF-1 gene was studied in 115 Etawah grade goats blood samples. A primer designed specifically was used to amplify a fragment DNA of IGF-1 locus along 249 bp. Restriction with SnaBI revealed one allele B was cut at one restriction site in fragment 249 bp. The frequencies for allele B was 1.00, resulting monomorphic allele. Based on Ge et al. (2001) and Siadkowska et al. (2006) researchs, allele B was associated on weight and body size.
Keyword: polymorphism, PCR-RFLP, IGF-1 gene, Etawah grade goats, Bogor Agricultural University (IPB)

Judul: Keragaman Gen CSN3 Ekson 4 pada Kambing Peranakan Etawah di BPTU KDI-HPT Pelaihari Kalimantan Selatan
Abstrak: Etawah grade goat as one of Indonesian local animal genetic resources has advantages, one of which is its high milk yield. CSN3 gene is a member of casein fragment related to protein and casein contents. The aim of this study was to identify polymorphisms of CSN3 in Etawah grade goat from BPTU KDI-HPT Pelaihari, South Kalimantan using the PCR-RFLP method. Polymorphisms of CSN3 gene were identified of 115 blood samples using PstI restriction enzyme that cuts the fragment of CTGCA|G. CSN3 amplification resulted 407 bp which is located at exon 4 was performed with annealing temperature 60 °C for 20 seconds. The results of PCR-RFLP analysis indicated that the analyzed CSN3 gene of Etawah grade goat which have monomorphic F allele was 100%.
Keyword: PCR-RFLP, Etawah grade goats, CSN3 gene

Judul: Proses Perancangan Lanskap House of Woodenuk Sultan of Johor, Singapura
Abstrak: Preservasi House of Woodneuk Sultan of Johor oleh Sultan Johor, Malaysia merupakan salah satu upaya dari pihak Malaysia dalam melindungi lanskap bersejarah yang memiliki nilai penting bagi Kerajaan Johor. Lokasi House of Woodneuk Sultan of Johor adalah di Singapura yang merupakan bekas wilayah kekuasaan Johor, sehingga saat ini Woodneuk menjadi lanskap yang memiliki nilai sejarah baik bagi Singapura maupun bagi Johor (Malaysia). House of Woodneuk Sultan of Johor, Singapore merupakan salah satu proyek yang sedang dikerjakan oleh AECOM International (Singapore). Sebagai kawasan bersejarah, perancangan kawasan ini sangat mempertimbangkan aspek orisinalitas dari nilai sejarahnya dan juga mengintegrasikan dengan konsep lanskap tema English Garden serta gaya hidup modern saat ini. English garden merupakan konsep gaya taman di Inggris yang menekankan pada setting alami nuansa pedesaan. AECOM merupakan akronim dari Architecture, Engineering, Consulting, Operations and Management. AECOM adalah sebuah perusahaan konsultan internasional yang memberikan pelayanan dalam bidang jasa teknik profesional dan manajemen. Ruang lingkup perusahaan ini adalah arsitektur, teknik bangunan, perencanaan, desain, dan pengembangan, ekonomi, energi, lingkungan, geoteknik, pemerintah, pengelolaan program transportasi, pembangunan perkotaan dan air.
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