Judul: Development of selection method corn parent inbred lines for parents at hybrid waxy corn tolerant to drought and introgression of opaque-2 (oo) gene by using SSRS (Simple Sequence Repeats)
Abstrak: The Dissertation study the development of selection method corn parent inbred lines for parents at hybrid waxy corn tolerant to drought and introgression of opaque- 2 (oo) gene by using SSRs (Simple Sequence Repeats). The first experiment was to determine relation of waxy corn genotype kinship based on DNA band pattern by using SSRs marker and correlation among taxonomic distance matrix averages based on morphological performance, and similarity coefficient based on DNA band pattern getting from SSRs marker. The result showed that genetic variance of the tested material was high enough. It was indicated by polymorphism value of 0.62. Dendogram construction based on UPGMA was able to distinguish one genotype to the others. According to genetic similarity, the genotypes could be grouped into three clusters characterized by the genotypes with similar pedigree code with cofenetic correlation coefficient (r) 0.88 categorized good fit for handled waxy corn genotype groups. However, Correlation value between taxonomic distance matrix based on morphological performance and similarity coefficient based on DNA band pattern according to goodness of fit criteria was categorized as very weak. The information could used in selecting the parent and become as one of strategies in hybrid maize breeding program. The second experiment was conducted to estimate combining ability and heterosis value of ten waxy corn genotypes having high amelopectin, tolerance to drought stress environment as parent candidate of hybrid formation. The result indicated that several genotypes having good combining ability value for grain weight character per plant and interval silking anthesis under stress and drought stress environment. Grain weight character per plant on without stress and drought stress environment showed the same combining ability value, while interval silking anthesis on without stress and drought stress environment revealed different combining ability value. This information is important to determine direct or indirect selection method in selecting maize genotypes which tolerant to drought stress environment. The third experiment was conducted to introgress recessive gene opaque-2 mutant from QPM genotypes to waxy corn genotypes by using MAS (Marker Assisted Selection). The result showed that specific SSRs marker phi057 was proved effective and efficient to be used in selecting genotypes of CML141 x PTBC4-7-1- BB and CML142 x PTBC4-10-1-BB progenies that were introgressed with homozygote recessive opaque-2 (oo) gene. Selection result utilizing MAS had a tendency to follow relative segregation on every selfing generation and the number of CML141 x PTBC4-7-1-BB and CML142 x PTBC4-10-1-BB progenies were screen with phi057 specific marker. Was identified 57 genotypes of PTBC4-7-1- BBo2 and PTBC4-10-1-BBo2 as genetic materials in development of hybrid maize variety having high amilopectin and protein content. The fourth experiment was carried out to obtain information on yield potency and waxy corn genotypes having opaque-2 gene as parent candidate of hybrid variety development. The result showed that the tested genotypes had good specific combining ability value good enough to one of the good testers either with CML144 or CML156 for grain weight character per plant, and grain weight character performance of top crossing 57 genotypes with the two very varied testers. The effect of environment factor was more dominant than
Keyword: Seleksi Galur Murni, Jagung Pulut, waxy corn, Simple Sequence Repeats

Judul: Genetic distance analysis based on ssrs and morphological marker and combining ability analysis for sweet corn development (Zea mays L. Var. Saccharata) hybrid.
Abstrak: The objective of the research were to grouping sweet corn lines resistant to downy mildew, high specific and general combining ability and high heterosis to select parental line to breed single cross hybrid of sweet corn. The first research was done to study genetic variability of 39 sweet corn lines based on SSR and morphological marker to select good parental lines of hybrid sweet corn. There were high genetic variability among genetic material, showed by genetic distance value about 0.18 to 0.78, indicates wide genetic variability of sweet corn. The results shows that among 20 lines having genetic similarity between 0.25 – 0.82 or genetic distance from 0.18 to 0.75; based on morphological trait 0.27 – 1.00. Correlation value between similarity matrix based on SSR and morphology was high on 0.88 of goodness of fit criteria. The aim of second research was to study general combining ability of sweet corn lines for yield and resistant to downy mildew for hybrid varieties improvement. Six of 45 lines having high fresh ear yield per plot and best general combining ability, such as Mr4/SC/BC4-2-1B, Mr4/SC/BC4-6-1B, Mr11/SC/BC4-2-1B, Mr11/SC/BC4-4-1B, Mr12/SC/BC4-6-1B, and Mr12/SC/BC3-3-1B. All of testing lines have resistance to downy mildew with severity about 0.0 to 7.1%. The lines having high GCA value would be good combiner to other lines to develop high yielding hybrid sweet corn and resistant to downy mildew. The third research was done to study general and specific combining ability of sweet corn lines and higher heterosis of downy mildew resistance supporting the hybrid corn development. The combination of A x D lines (Mr12/SC/BC4-6-1B x Mr11/SC/BC4-2-1B) were medium genetic distance and having highest GCA, heterosis and heterobeltiosis for fresh ear yield per plot character. The D x C combination (Mr11/SC/BC4-2-1B x Mr4/SC/BC4-2-1B) have wide genetic distance and high fresh ear yield per plot, heterosis and heterobeltiosis, but have negative SCA value. The combination of E x C (Mr12/SC/BC3-3-1B x Mr4/SC/BC4-2-1B) have a wide genetic distance but low productivity. The best hybrid is not always obtained from parents with the high genetic distance, but also can be obtained from the low or medium genetic distance as well.
Keyword: Hybrid sweet corn, Zea mays L. var. saccharata, Genetic variability

Judul: Kajian Persyaratan Penempatan Jabatan Struktural Eselon Iv dan Iii Di Dinas Kebakaran Dki Jakarta
Abstrak: Dinas Kebakaran Daerah Khusus Ibukota (DKI) Jakarta adalah perangkat pelaksana Pemerintah DKI Jakarta dalam bidang pelaksanaan usaha pencegahan dan penanggulangan kebakaran serta perlindungan keselamatan jiwa termasuk harta benda dari bahaya kebakaran dan bencana lainnya di wilayah Ibukota Jakarta. Dalam melaksanakan tugas pokok dan fungsinya, Dinas Kebakaran DKI memiliki kewenangan dan tanggung jawab, sumberdaya, dana, sarana, prasarana dan personil yang memerlukan keahlian dan keterampilan, peralatan dan perlengkapan yang diatur dalam Peraturan Daerah (Perda) Nomor 9 Tahun 1980 Juncto Nomor 11 tahun 1986. Dimensi dan kompleksitas permasalahan kebakaran, dalam hal ini adalah kebakaran. kota merupakan salah satu bencana bagi di wilayah Kota DKI Jakarta. Terjadinya kebakaran kota semakin lama semakin bertambah seiring dengan perkembangan Jakarta sebagai kota metropolitan. Tingginya insiden-insiden yang terjadi pada beberapa periode belakangan ini, baik variasi jenis maupun penyebab dan akibat kerugian yang ditimbulkannya, memperlihatkan kecenderungan yang juga semakin meningkat yang secara keseluruhan masih merupakan kerugian yang langsung dapat dihitung, belum termasuk kerugian tak langsung sebagai akibat terjadinya insiden-insiden tersebut. Oleh karena itu, Dinas Kebakaran DKI Jakarta selaku organisasi yang berkompeten dalam penanggulangannya harus mengambil langkah-langkah antisipatif yang diperlukan agar pelaksanaan tugas yang berada dalam kondisi yang baik dapat dipertahankan atau bahkan ditingkatkan. Lajunya perkembangan ilmu pengetahuan dan teknologi (IPTЕК), menghadapkan Dinas Kebakaran DKI Jakarta kepada berbagai permasalahan, terutama masalah rendahnya kualitas pelayanan kepada masyarakat. Masalah ini dirasakan oleh masyarakat karena adanya kebijakan zero growth sampai tahun 2000 oleh Menteri Pembinaan Aparatur Negara, sehingga membawa konsekuensi logis bagi unit instansi masing-masing untuk meningkatkan produktivitas kerja dengan memberdayakan sumberdaya manusia yang ada. Usaha untuk meningkatkan produktivitas kerja harus disertai dengan pendidikan dan pelatihan atau dengan perpaduan pandangan antara karyawan dengan pimpinan untuk menciptakan kerjasama yang kondusif, pembinaan efektivitas kerja ke arah pengaturan dan pengusahaan secara maksimal dengan penempatan karyawan atau pegawai atas prinsip penempatan orang yang tepat pada tempat yang tepat (tke right man on the ringt job). Dalam upaya tersebut di atas, Dinas Kebakaran DKI Jakarta telah membuat standar jabatan dengan mengacu kepada National Fire Protection Association (NFPA). Namun demikian, standar jabatan tersebut berbenturan dengan peraturan-peraturan yang berlaku di Pememerintah DKI Jakarta. Benturan tersebut diantaranya adalah untuk karier dalam jabatan struktural Eselon IV dan III harus mengikuti dan lulus dari Diklat ADUM (Administrasi Umum) dan SPAMA (Staf dan Pimpinan Administrasi Tingkat Pertama). Hal ini tentunya membawa dampak tersendiri bagi Dinas Kebakaran DKI Jakarta dan mempengaruhi pelaksanaan penanggulangan kebakaran. Sebagaimana yang tercantum dalam Perda Nomor 11 Tahun 1986 mengenai struktur organisasi Dinas Kebakaran DKI Jakarta, sebagai 'ujung tombak Dinas Kebakaran adalah Kepala Kepala Suku Dinas Kebakaran di lima Wilayah Kotamadya. Untuk meningkatkan kemampuan manajerial dan teknisnya, pejabat-pejabat tersebut hendaknya dibekali kemampuan manajerial dan kemampuan tehnik sebagai fire fighter (petugas pemadam kebakaran) dan penguasaan wilayah kota untuk pengawasan penanggulangan kebakaran. Keberhasilan Dinas Kebakaran dalam melaksanakan tugas pokok dan fungsi akan tercapai apabila didukung oleh peran sumberdaya manusia yang dimiliki. Oleh karena itu, mengingat pentingnya peran sumberdaya manusia dalam tugas operasional Dinas Kebakaran, maka perlu diupayakan adanya suatu rancang bangun sistem pengembangan karier melalui kajian persyaratan penempatan jabatan struktural Eselon IV dan III di Dinas Kebakaran DKI Jakarta. Berdasarkan latar belakang di atas dan
Keyword: Jabatan Struktural, Dinas Kebakaran