Judul: Pengaruh Bauran Pemasaran terhadap Keputusan Pembelian Rumah di Perumahan Kuningan City View (KCV) Kabupaten Kuningan.
Abstrak: Peningkatan sektor perumahan ditentukan oleh keputusan pembelian konsumen yang dipengaruhi oleh bauran pemasaran. Perumahan Kuningan City View (KCV) sebagai salah satu perumahan yang sedang berkembang harus mampu menentukan faktor bauran pemasaran yang tepat sehingga dapat menarik konsumen untuk melakukan pembelian. Penelitian ini bertujuan menganalisis pengaruh faktor-faktor bauran pemasaran terhadap keputusan pembelian rumah pada perumahan KCV dan menganalisis faktor bauran pemasaran yang berpengaruh dominan terhadap keputusan pembelian rumah di perumahan KCV. Penelitian ini dilakukan dengan menggunakan analisis Structural Equation Modeling (SEM) dengan pendekatan Partial Least Square (PLS). Hasil dari penelitian ini menunjukkan bahwa variabel lokasi dan promosi dari bauran pemasaran berpengaruh positif dan signifikan terhadap keputusan pembelian rumah di perumahan KCV. Variabel bauran pemasaran yaitu lokasi memiliki pengaruh paling dominan terhadap keputusan pembelian pada perumahan KCV Kabupaten Kuningan.
Keyword: Bauran Pemasaran, Keputusan Pembelian, SEM-PLS

Judul: Pengaruh Bauran Promosi Terhadap Keputusan Pembelian Rumah di Dago Village Bandung
Abstrak: Rumah merupakan kebutuhan primer yang harus dipenuhi oleh semua orang untuk tempat berteduh dan beristirahat. Dago Village merupakan perusahaan di Bandung yang bergerak dibidang properti, dengan promosi bertema pedesaan dapat mempengaruhi persepsi konsumen untuk membeli rumah. Tujuan dari penelitian ini ialah menganalisis karakteristik konsumen Dago Village, menganalisis persepsi konsumen Dago Village, menganalisis kegiatan promosi apa yang paling berpengaruh terhadap keputusan konsumen membeli rumah di Dago Village, menganalisis pengaruh bauran promosi terhadap keputusan pembelian. Data primer yang digunakan diperoleh dengan penyebaran kuesioner pada konsumen yang telah membeli rumah serta yang pernah berkunjung dan diolah menggunakan analisis deskriptif dan SEM smartPLS. Pada tahapan keputusan pembelian sebesar 83.6 persen konsumen merasa bahwa bauran promosi berpengaruh dalam keputusan pembelian, Faktor yang paling berpengaruh dalam keputusan pembelian rumah di Dago Village ialah promosi penjualan yang ditujukan langsung kepada keluarga, teman dan rekan kerja konsumen.
Keyword: bauran promosi, SEM, keputusan pembelian

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable