Judul: Identifikasi Keragaman Gen Miostatin pada Domba Lokal
Abstrak: Myostatin is a member of tranforming grown factor B superfamily, know as growth and differentiation factor, which is essensial for proper regulation of skeletal muscle mass in wide range vertebrate species. To pursue potential agricultural application of increasing muscle mass by inhibition of myostatin activities, the aim of this study was to identify the variants of myostatin gene in the Indonesian local sheeps. A total of 151 DNA samples belonging to eight different areas that are Bogor Ciomas (20), Bogor Jonggol (35), Margawati (18), Indramayu (30), Dinggala (24), MAdura (8), Sumbawa (8), and Rote (8) were analyzed with Polymerase Chain Reaction-Single Starnd Conformation Polymorphism. The Myostatin (GDF 8) amplified product was 688 bp(base pair)
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Judul: Identifikasi Keragaman Gen Calpastatin (CAST-NcoI) dengan Teknik PCR-RFLP pada Domba Lokal Indonesia
Abstrak: Domba lokal di Indonesia memiliki beberapa keunggulan, yaitu mampu bertahan pada iklim tropis, bersifat prolifik, tidak mengenal musim kawin, tahan terhadap beberapa macam penyakit dan parasit. Namun, produktivitas domba lokal masih cukup rendah terutama sifat pertumbuhan dan kualitas daging. Salah satu upaya seleksi dilakukan untuk meningkatkan produktivitas domba lokal yaitu berdasarkan pada penciri DNA yang dikenal dengan Marker Asissted Selection (MAS). Gen calpastatin merupakan gen yang berfungsi menghambat degradasi protein sel-sel otot. Pada mamalia, gen calpastatin diduga memiliki keterkaitan dalam sifat pertumbuhan otot dan keempukan daging. Tujuan dari penelitian ini adalah untuk mengidentifikasi keragaman gen calpastatin pada domba lokal . Sampel DNA yang digunakan dalam penelitian ini berjumlah 232 sampel, yang terdiri dari Domba Ekor Tipis (DET) Jonggol (38), DET Rote (25), domba Garut Ciomas (25), domba Garut Margawati (23), domba Garut Wanaraja (25), Domba Ekor Gemuk (DEG) Indramayu (25), DEG Madura (25), DEG Donggala (25), dan DEG Sumbawa (21). Amplifikasi gen calpastatin dilakukan dengan teknik PCR, sedangkan untuk menentukkan genotipenya dilakukan dengan teknik Restriction Fragment Length Polymorphism (RFLP) menggunakan enzim restriksi NcoI. Analisis data dilakukan dengan menghitung nilai frekuensi alel dan frekuensi genotipe.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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