Judul: Pengaruh Menonton Film dan Bermain Video Game Kekerasan terhadap Perilaku Agresi Siswa SMP di Perdesaan Bogor
Abstrak: Menonton film dan bermain video game berunsur kekerasan memberikan dampak bagi peminatnya, salah satunya perilaku agresi. Penelitian ini bertujuan untuk menganalisis pengaruh menonton film dan bermain video game kekerasan terhadap perilaku agresi siswa SMP di perdesaan Bogor. Lokasi penelitian terletak di Desa Ciasihan dan Ciasmara, Kecamatan Pamijahan, Kabupaten Bogor. Penelitian ini menggunakan desain cross sectional study dengan melibatkan 60 siswa SMP kelas VII dan VIII yang memiliki orang tua lengkap yang dipilih secara proportional random sampling. Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan yang nyata pada film kekerasan, video game kekerasan dan perilaku agresi antara laki-laki dan perempuan. Uji korelasi menunjukkan bahwa menonton film dan bermain video game kekerasan berhubungan positif signifikan dengan perilaku agresi. Hasil uji regresi linear berganda menunjukkan bahwa menonton film dan bermain video game kekerasan memiliki pengaruh yang positif signifikan terhadap perilaku agresi.
Keyword: Agresi, Film kekerasan, Perdesaan, Remaja, Video game kekerasan

Judul: Relationship between behavior of watching Violence films with adolescence aggression behavior: Case adolescence of SMK Pelita Kecamatan Ciampea, Kabupaten Bogor, Provinsi Jawa Barat.
Abstrak: Beberapa tahun terakhir ini adegan kekerasan banyak ditayangkan dalam perfilman. Perkelahian, pemukulan, pembunuhan dan sebagainya yang merusak dan merugikan orang lain selalu muncul dalam film. Meningkatnya proporsi adegan kekerasan dalam film-film melahirkan kecaman timbulnya pengaruh negatif bagi penonton. Kecemasan munculnya pengaruh negatif semakin bertambah karena karakteristik penonton yang didominasi remaja. Salah satu dugaan dampak negatif film pada remaja adalah perilaku agresi. Perilaku agresi menurut Baron (Koeswara, 1988) adalah tingkah laku yang ditunjukkan untuk melukai dan mencelakakan individu lain yang tidak menginginkan datangnya tingkah laku tersebut. Tujuan penelitian ini adalah (a) mengetahui perilaku remaja dalam menonton film kekerasan (b) mengetahui perilaku remaja dalam berperilaku agresi (c) menganalisa hubungan antara perilaku remaja dalam menonton film kekerasan dengan perilaku agresi. Populasi dalam penelitian ini adalah seluruh siswa di SMK Pelita Ciampea. Jumlah responden yang ditentukan untuk penelitian ini sebanyak 45 responden. Data diolah dengan menggunakan Chi-Square test. Hasil uji Chi-Square kemudian digunakan untuk melihat keeratan hubungan antara dua variabel dengan rumus koefisien kontingensi (C). Makin besar C berarti hubungan antar dua variabel makin erat. Nilai C berkisar 0-1. Dari penelitian yang dilakukan, dapat disimpulkan bahwa intensitas remaja dalam menonton film kekerasan pada umumnya jarang. Sementara jenis film kekerasan yang lebih sering ditonton adalah film horor. Media film yang sering digunakan oleh responden adalah televisi dan VCD/DVD Player. Pada umumnya responden mendapatkan film kekerasan dengan cara membelinya.
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Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable