Judul: Pemahaman Siswa terhadap Fenomena Perubahan Iklim (Studi kasus: Kabupaten Lembata dan Timor Tengah Utara).
Abstrak: Informasi mengenai perubahan iklim bagi anak-anak sangat bermanfaat untuk mengantisipasi potensi dampak yang ditimbulkan. Namun, pemahaman siswa SMP dan SMA terhadap fenomena perubahan iklim berdasarkan data tahun 2016 untuk Kabupaten Lembata dan Timor Tengah Utara masih minim, meskipun sebanyak 98% siswa mengaku merasakan adanya perubahan dalam lingkungan mereka. Penelitian ini dilakukan menggunakan konsep Climate Change Awareness Index (CAI). Persentase siswa dengan tingkat pemahaman cukup dan kurang sebesar 89% dan 11% dan tidak ada responden dengan tingkat pemahaman yang baik. Faktor sosial-ekonomi mempengaruhi pemahaman siswa ditunjukkan dengan adanya perbedaan signifikan pada nilai rata-rata CAI siswa dengan kondisi sosial-ekonomi yang berbeda. Selain itu, sumber informasi juga mampu mempengaruhi pemahaman anak terhadap fenomena perubahan iklim. Pendidikan formal di sekolah merupakan salah satu sumber informasi mengenai perubahan iklim. Walaupun demikian, kesesuaian informasi perubahan iklim dalam buku pelajaran di Indonesia disandingkan dengan rekomendasi UNICEF belum mencapai 50%, sehingga belum cukup untuk memberikan pemahaman perubahan iklim bagi siswa. Sehingga perlu adanya perbaikan dalam penyediaan dan akses terhadap informasi iklim bagi anak-anak.
Keyword: awareness index, pemahaman, perubahan iklim, siswa

Judul: The Influence of Knowledge, Awareness, Responsibility, and Environmental Attitudes on Ecological Behavior of School-Age Children
Abstrak: Permasalahan lingkungan yang masih terjadi di Indonesia adalah masalah sampah dan penggunaan energi yang berlebihan. Di sisi lain, pemerintah telah mengadakan program sekolah Adiwiyata sebagai bentuk implementasi pembelajaran tentang lingkungan hidup di ranah sekolah. Penelitian ini bertujuan untuk menganalisis pengaruh pengetahuan, kesadaran, tanggung jawab, dan sikap lingkungan terhadap perilaku ekologis anak usia sekolah. Penelitian ini menggunakan desain penelitian cross sectional study dan teknis penarikan contoh menggunakan probability sampling dengan metode cluster random sampling. Data dikumpulkan dengan membagikan kuesioner penelitian yang diisi sendiri oleh responden (self-administered) dengan pendampingan. Responden dalam penelitian ini merupakan siswa SD kelas 4, 5, dan 6 dengan jumlah 207 siswa. Analisis data deskriptif dan uji korelasi menggunakan SPSS, sedangkan untuk uji SEM menggunakan Smart PLS. Hasil uji korelasi menunjukkan bahwa terdapat hubungan antara pendidikan terakhir ayah dan ibu, serta pekerjaan ayah dengan perilaku ekologis. Analisis SEM menunjukkan bahwa tanggung jawab lingkungan berpengaruh positif signifikan secara langsung terhadap sikap lingkungan. Pengetahuan lingkungan, tanggung jawab lingkungan, dan sikap lingkungan memiliki pengaruh langsung yang positif signifikan terhadap perilaku ekologis. Perilaku ekologis anak dapat ditingkatkan dengan cara membiasakan diri mengurangi limbah sampah, membuang sampah pada tempatnya, menghemat energi listrik dan air, serta melakukan daur ulang sampah., Environmental problems that still occur in Indonesia are waste and excessive energy use. On the other hand, the government has held the Adiwiyata school program as a form of implementation of learning about the environment in the school realm. This study aims to analyze the influence of knowledge, awareness, responsibility, and environmental attitudes on the ecological behavior of school-age children. This study used a cross sectional study design and sampling technique using probability sampling with cluster random sampling method. Data were collected by distributing research questionnaires that were filled out by the respondents themselves with assistance. Respondents of this study were elementary school students in grades 4, 5, and 6 with a total of 207 students. Descriptive data analysis and correlation tests used SPSS, while the SEM test used Smart PLS. The correlation test results show that there is a relationship between the last education of the father and mother, as well as the father's occupation with ecological behavior. SEM analysis shows that environmental responsibility has a significant positive effect directly on environmental attitudes. Environmental knowledge, environmental responsibility, and environmental attitudes also have a significant positive direct effect on ecological behavior. Children's ecological behavior can be improved by getting used to reducing waste, throwing garbage in its place, saving electricity and water, and recycling waste.
Keyword: ecological behavior, environmental attitudes, environmental awareness, environmental knowledge, environmental responsibility

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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