Judul: Fungsi Kognitif, Interaksi Sosial, dan Konsep Diri Lansia Janda dan Duda.
Abstrak: Lanjut usia yang berada pada tahap akhir kehidupan, mengalami perubahan besar seperti perubahan fisik, psikologis, maupun social yang dapat memengaruhi ketidakstabilan konsep diri. Penelitian ini bertujuan untuk menganalisis pengaruh fungsi kognitif dan interaksi sosial terhadap konsep diri lansia janda dan duda. Penelitian ini bertempat di Desa Timbulharjo, Kecamatan Sewon, Kabupaten Bantul, Yogyakarta yang dipilih secara purposive dengan pertimbangan wilayah yang paling banyak jumlah penduduk lansia. Lansia dalam penelitian ini adalah lansia yang berusia lebih dari 60 tahun yang sudah ditinggalkan oleh pasangannya lebih dari atau sama dengan dua tahun. Jumlah contoh dalam penelitian ini sebesar 30 orang lansia janda dan 30 orang lansia duda. Hasil penelitian menunjukkan tidak ada perbedaan fungsi kognitif, dan interaksi sosial antara lansia janda dan lansia duda, namun terdapat perbedaan konsep diri antara lansia janda dan duda. Selain itu, usia dan lama pendidikan memiliki hubungan dengan fungsi kognitif, dan interaksi sosial memiliki hubungan dengan konsep diri. Sementara itu, status perkawinan berpengaruh negatif dan interaksi sosial berpengaruh positif terhadap konsep diri lansia sehingga status janda dapat menurunkan konsep diri lansia, dan semakin baik interaksi sosial maka konsep diri lansia akan semakin baik.
Keyword: fungsi kognitif, interaksi sosial, konsep diri, lansia janda dan duda

Judul: Interaksi Sosial, Strategi Koping, dan Kesejahteraan Subjektif Lansia di Masa Pensiun
Abstrak: Penelitian ini bertujuan untuk menganalisispengaruh interaksi sosial, strategi koping, dan kesejahteraan subjektiflansia di masa pensiun. Desain penelitian yang digunakan adalah cross-sectional study. Penelitian dilakukan di Kelurahan Cilendek Barat, Kecamatan Bogor Barat. Jumlah contoh dalam penelitian ini berjumlah 60 orang lansia. Hasil penelitian menunjukkan tidak terdapat perbedaan nyata skor pencapaian interaksi sosial, strategi koping, dan kesejahteraan subjektif pada lansia laki-laki dan perempuan. Usia dan lama pensiun berhubungan negatif dengan interaksi sosial. Usia, lama pensiun, dan jumlah anak berhubungan positif dengan strategi koping. Terdapat hubungan dan pengaruh positif signifikan interaksi sosial terhadap kesejahteraan subjektif.
Keyword: interaksi sosial, kesejahteraan subjektif, lansia, pensiun, strategi koping

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable