Judul: Profil imunohistokimia antioksidan superoksida dismutase (SOD) pada usus halus tikus yang diberi probiotik dan enteropathogenic e. coli (EPEC)
Abstrak: The study was conducted to analyse the influence of probiotic Lactobacillus plantarum and Lactobacillus fermentum on immunohistochemical profile of antioxidant superoxide dismutase (Cu,Zn-SOD) in small intestine of Enteropathogenic E. coli (EPEC)-treated Rats. A total of 90 male rats (Sprague Dawley) were used in this study. They were devided into 6 groups; negative control group (A), Lactobacillus plantarum treated group (B), Lactobacillus fermentum treated group (C), Lactobacillus plantarum and EPEC treated group (D), Lactobacillus fermentum and EPEC treated group (E), and EPEC treated group (F). The content of antioxidant cooper,zinc superoxide dismutase (Cu,Zn-SOD) were detected immunohistochemically using monoclonal antibody of Cu,Zn-SOD. The result showed that Lactobacillus fermentum treatment increased the content of Cu,Zn-SOD in rats small intestine at both condition; without EPEC treatment and with EPEC treatment. However Lactobacillus plantarum increased the content of Cu,Zn-SOD in rats small intestine only at condition without EPEC treatment. While, at EPEC treated rats, the lactobacillus plantarum treatment could maintain the content of Cu,Zn-SOD as well as negative control group. Lactobacillus fermentum showed better effect on increasing the content of Cu,Zn-SOD in rats small intestine.
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Judul: Efek probiotik indigenus pada profil imunohistokimia antioksidan superoksida dismutase (SOD) di hati tikus yang dipapar enteropathogenic Escherichia coli (EPEC)
Abstrak: The study was conducted to analyze influence of indigeneous probiotic Lactobacillus plantarum and Lactobacillus fermentum on immunohistochemical profile of antioxidant superoxide dismutase (SOD) in liver of enteropathogenic Escherichia coli (EPEC) treated rats. A total of 90 male rats (Sprague Dawley) were divided into six groups; (A) negative control, (B) Lactobacillus plantarum treated group, (C) Lactobacillus fermentum treated group, (D) Lactobacillus plantarum and EPEC treated group, (E) Lactobacillus fermentum and EPEC treated group, and (F) EPEC treated group (positive control). The content of antioxidant copper, zinc-superoxide dismutase (Cu,Zn-SOD) was analyzed with immunohistochemical techniques using monoclonal antibody of Cu,Zn-SOD. The result showed that L. fermentum increased the content of Cu,Zn-SOD in rat liver on 2nd and 3rd weeks at both conditions; with and without EPEC treatment. L. plantarum treatment for 1 week until 3 weeks, could maintain the content of Cu,Zn-SOD in rat liver tissue as well as negative control. While, in L. plantarum and EPEC treated group, the content of Cu,Zn-SOD decreased after one week of EPEC treatment. L. fermentum showed better effect in terms increase the content of Cu,Zn-SOD in rat liver tissue.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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