Judul: DNA Barcode as an Alternative of Mantis Shrimp Species Identification
Abstrak: Udang mantis atau yang dikenal dengan udang ronggeng merupakan salah satu anggota Subfilum Crustacea Ordo Stomatopoda, Beberapa spesies udang mantis dikenal sebagai komoditi ekspor dan makanan eksotis. Beberapa udang mantis yang bernilai ekonomi tinggi adalah anggota famili Harpiosquillidae dan Squillidae. Udang-udang mantis tersebut biasa ditangkap dari Laut Jawa dan Laut Cina Selatan. Penelitian ini bertujuan untuk mengaplikasikan teknik DNA barcoding sebagai laternatif mengidentifikasi udang mantis sampai ke tingkat spesies. Sebanyak 34 sampel udang mantis berasal dari Jambi, Cirebon, dan Aceh yang digunakan adalah spesimen koleksi yang diawetkan dalam alkohol. Identifikasi spesies dilakukan berdasarkan morfologi. Ekstraksi DNA dilakukan dari otot tungkai dan ruas gen CO1 diamplifikasi sebagai ruas DNA yang dijadikan barcode. Amplifikasi gen CO1 berhasil dilakukan untuk semua sampel yang kesemuanya menghasilkan amplikon yang multiband. Perunutan nukelotida hanya berhasil dilakukan untuk tujuh sampel, yaitu Harpiosquilla harpax (Sampel No. 34 dan No. 37 asal Jambi), Harpiosquilla stephensoni (sampel No. 1 dan No. 2 asal Cirebon), dan Carinosquilla multicarinata (sampel No. 9 dan No. 11 asal Cirebon, dan No. 22 asal Aceh). Berdasarkan ruas gen CO1, C. multicarinata asal Aceh memiliki kekerabatan yang berbeda dengan C. multicarinata asal Cirebon.
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Judul: Pola Distribusi Geografis Pada Udang Mantis Di Laut Jawa Berdasarkan Genom Mitokondria
Abstrak: Mantis shrimp or ronggeng shrimp (Harpiosquilla harpax) is a large sized shrimp. The mostly habitat of mantis shrimp is beach. Shrimps are exploited for trade as an exotic food. Shrimp liked as a source of food because large size and presentation of meat can be as high as 50% by weight. Characteristics stomatopod as predators have two methods to catch prey are smashers and spearers. H. Harpax classified into spearers. This research was conducted to analyze the genetic diversity of mantis shrimp H. harpax in Java Sea and study the distribution based on mitochondrial genome. The sample used is the mantis shrimp H. harpax many as eight tails collection of Dr. Yusli Wardiatno from the Department of Aquatic Resources and Management IPB preserved in ethanol. Amplification was tested in vitro with the technique of Polymerase Chain Reaction (PCR). Product amplification is 1100 bp were arranged and phylogeni analyzed. All of the eight sample are successfully amplified. The distribution pattern of mantis shrimp larvae is mainly larvae carried of alongshore current shoreline. According by filogeografi H. harpax in Cirebon water separately with H. harpax in Vietnam. This is evidenced by the value of the farthest genetic distance is between H. harpax Vietnam and H. harpax 3 is 10.5%, while the closest genetic distance was between H. harpax 1 and 6 (group 1) is 1.8%. Genetic Diversity of H.harpax in Cirebon Aquatic based on d-loop segment has a greater value is 5.1% (± 0.005).
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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