Judul: African Swine Fever Virus Detection Using Gel Based Polymerase Chain Reaction
Abstrak: African Swine Fever (ASF) diyakini sebagai salah satu penyakit menular babi yang paling berbahaya dan menyebabkan kematian yang tinggi pada babi. Gejala klinis maupun pemeriksaan postmortem ASF sulit dibedakan dari Classical Swine Fever (CSF) dan penyakit hemoragik lainnya. Karenanya uji laboratorium penting dilakukan untuk mengetahui langkah yang tepat dalam pengobatan dan pengendaliannya. Penelitian bertujuan mendeteksi virus ASF di organ (paru-paru, hati, dan ginjal) babi menggunakan teknik Polymerase Chain Reaction (PCR) berbasis gel dengan primer P72-U dan P72D sebagai langkah peneguhan diagnosis penyakit. Metode penelitian ini terdiri dari beberapa tahapan seperti preparasi sampel, isolasi DNA virus, uji PCR, elektroforesis, dan visualisasi hasil. Hasil menunjukkan reaksi positif pada semua sampel organ tetapi pita amplifikasi sampel ginjal terlihat lebih tipis yang kemungkinan disebabkan oleh konsentrasi DNA virus yang relatif lebih sedikit pada ginjal. Penelitian lebih lanjut diperlukan untuk mengetahui jumlah konsentrasi virus dan kepastian penyebab tipisnya pita.
Keyword: African swine fever, pig, PCR

Judul: Pathological Overview of African Swine Fever (ASF) in Pigs (Sus scrofa domesticus)
Abstrak: Babi domestik merupakan salah satu ternak potensial yang semakin meningkat produksinya. Maraknya kasus African Swine Fever (ASF) mengakibatkan berbagai kerugian antara lain kerugian sosial, ekonomi, bahkan lingkungan. Berdasarkan permasalahan tersebut, perlu diketahui lebih lanjut gambaran patologi penyakit ASF. Studi kasus ini bertujuan mempelajari gambaran patologi ASF pada babi (Sus scrofa domesticus). Penelitian dilakukan dengan nekropsi pada babi, beberapa organ dan bagian diamati. Kemudian diambil sampel organ sebagai preparat histopatologi dengan pewarnaan hematoksilin eosin (HE). Perubahan dalam patologi anatomi dan histopatologi kedua ekor babi mengindikasikan kejadian ASF. Perubahan patologi anatomi seperti hemoragi pada beberapa organ, meliputi otak, kulit, kelenjar pertahanan, dan limpa sebagai organ target utama. Data ditunjang dengan pemeriksaan melalui pewarnaan HE yang menunjukkan lesio-lesio ASF. Bukti kuat ditandai heemorrhagic splenitis dan infiltrasi sel radang pada limpa sebagai tanda patognomonik ASF. Berdasarkan hasil kedua pemeriksaan tersebut, babi terinfeksi ASF dengan tipe akut., Domestic pigs are one of the potential livestock whose production is increasing. The rise of cases of african swine fever (ASF) has resulted in various losses, incluiding social, economic and even environmental losses. Based on these problems, it is necessary to know more about the pathological features of ASF disease. This case study aims to study the pathological overview of ASF in pigs (Sus scrofa domesticus). The research was carried out by necropsy on pigs, observing several parts and organs. Then organ samples were taken as histopathological preparations stained with hematoxylin and eosin (HE). Changes in the anatomical pathology and histopathology of the two pigs indicate the occurrence of ASF. Anatomical pathological changes such as hemorrhage in several organs, including the brain, skin, defense glands, and spleen as the main target organs. The data was supported by examination through HE staining which showed ASF lesions. Strong evidence also supported by hemorrhagic splenitis and infiltration of inflammatory cell of the spleen as pathognomonic signs of ASF. Based on the results of the two examinations, pigs were infected with ASF of the acute type.
Keyword: akut, ASF, histopatologi, patognomonik, patologi anatomi

Judul: Hybrid solar cells of ZnO nanoparticles/chlorophyll was modified metal ions (Zn2+ and Cu2+ )
Abstrak: Zinc Oxide (ZnO) is a n-type semiconductor with a wide the energy band (Band Gap) is 3.37 eV at room temperature and 3.34 eV at low temperature with a large exiton binding energy is 60 meV. ZnO has been synthesized by hydrothermal method, resulting in a decrease in particle size over the duration of the hydrothermal changes. Crystallographic structure of ZnO were characterized using XRD, has shown the characteristic pattern of a hexagonal wurtzite structure, the crystallite size increases to changes in particle size. Changes in particle size has also led to a change in the absorption peak of ZnO as has been demonstrated posted under test using the optical properties of UV-Vis spectrophotometer. Changes of this uptake has also led to changes in the energy band gap, which increases its band gap energy of the particle size. In electronic applications, especially in solar cells. Chlorophyll is considered less effective, because of their very nature are easily degraded as a result of several reactions which are acid, lighting and temperature. The results of the stability test using a halogen lamp 34 W/m2 for 5 hours, that the substitution of chlorophyll with metal ions Zn2+ and Cu2+ has been shown to form more stable than natural chlorophyll. The characterization of the optical properties, the combination of ZnO / chlorophyll in the form of hybrid films has led to a shift in the absorption region of ZnO becomes more widely from the UV to Visible. ZnO/chlorophyll has absorption range of 350-515 nm, ZnO / Zn-feofitin 332-401 nm and 332-632 nm for Cu-feofitin. The combination of ZnO/chlorophyll and CuSCN as a solid electrolite in hybrid solar cell, a modified chlorophyll with metal ions Zn2+ and Cu2+ has been shown to form a more stable I-V curve. It is characterized by its fill factor value greater than natural chlorophyll. Nevertheless, solar cells with natural chlorophyll has been able to produce higher efficiency values of chlorophyll were substituted.
Keyword: Hydrothermal, ZnO/Chlorophyll modified, Hybrid Solar Cell