Judul: Pemetaan Perubahan Kerapatan Kanopi Hutan di Hutan Rakyat, Kabupaten Kuningan, Jawa Barat
Abstrak: Emisi karbon dioksida dari sektor kehutanan dapat berasal dari deforestasi dan degradasi hutan. Pengukuran deforestasi hutan lebih mudah dilakukan dari pada degradasi hutan. Kerapatan kanopi hutan (Forest Canopy Density/FCD) dapat menjadi variabel penduga dinamika degradasi hutan. Penelitian ini bertujuan untuk mencari hubungan keeratan antara Forest Canopy Density (FCD) dan parameter tegakan yang diukur di lapangan. Penelitian dilakukan di hutan rakyat Kabupaten Kuningan Jawa Barat. Analisis regresi linier sederhana menunjukan koefisisen determinasi terbesar adalah hubungan antara FCD dengan luas bidang dasar tegakan, sebesar 0.71. Persamaan regresi linier hubungan antara FCD dengan LBDT adalah persamaan ini dapat digunakan untuk memprediksi LBDT dengan data FCD. Nilai ketepatan dari model regresi linier ini sebesar 18.98%. Penelitian ini juga bertujuan untuk mengetahui perubahan kerapatan kanopi di hutan rakyat Kabupaten Kuningan pada tahun 1991-2002 dan tahun 2002-2009.
Keyword: degradasi, FCD, hutan, reforestasi, regresi

Judul: Pemetaan Perubahan Forest Canopy Density di KPH Kuningan
Abstrak: Changes in the quality of the forest, including forest degradation, can be estimated by measuring the density of the forest canopy. Forest canopy density (FCD) is influenced by variables such as leaf area index, basal area and stand density. The analysis of Landsat imagery acquired in 1991, 2002 and 2012 showed that FCD in KPH Kuningan area has changed. During the period of 1991- 2002, the decreased FCD was about 206.68 ha, meanwhile 4504.18 ha area has showed an increase. In the period of 2002-2012, the decreased FCD was 1826.72 ha and 3311.82 ha increase. Among the three variables tested, stand density has the strongest association with FCD (R ² = 0.79) and its relation was follow equation Kerapatan Tegakan (KT) = 7.05+4.73FCD. The error accuracy of prediction model was 6.2%, which means that the model has high accuracy
Keyword: stand density, leaf area index, forest degradation, forest canopy density, basal area

Judul: DNA Extraction and Characterization of Salmonella‟s Lytic Bacteriophage Genome
Abstrak: Bacteriophages are ubiquitous in nature and are known to proliferate wherever their bacterial hosts exist. Their virion particles can exist independently outside the host, however all phages are need their host to propagate. Several phages are highly specific to host cell surface receptors and any slight changes in structure results in little or no interaction between the phage and its host. Salmonella‟s phage isolated from sewage water where their bacteria exist. Three isolated of phages (phages 15, 19 and 38) were used in this research that concentrated using three types of methods. PEG/ NaCl method was firstly used to concentrate the phages based on their molecular weight to precipitate the phage particles during centrifugation. The second method was Scraping Plaque method. In this method, overlay plaques were scraped and collected in SM buffer to get phage concentrates. Enrichment method was the last method used to concentrate the phages. Phage genome isolation was done using two methods i.e. EDTA/SDS and Proteinase K methods. The first method was done using DNase I to eliminate bacterial DNA followed by SDS/EDTA treatment to release phages DNA and phenol-chloroform-isoamyl alcohol to extract the DNA. DNA was collected by isopropanol extraction. While in Proteinase K method, bacterial DNA was eliminated by DNase I and Proteinase K/SDS was used to degrade phages capsids and ethanol for DNA precipitation. The result showed that PEG/NaCl method resulted the highest phage concentration for phage 15 and phage 19, while scrapping method was the highest for phage 38. The quantity and purity of extracted DNA were low for PEG/NaCl and Scraping methods, while Enrichment method was higher indicated higher purity. Run on agarose gel electrophoresis of extracted DNA confirmed that Enrichment method results were the best while scraping and PEG/NaCl method was appeared smear background. Based on DNA genome size determination, the bacteriphages of Salmonella sp were comparable with other study of Salmonella phages. Phage P15 with genome size of 39,5 kb was close to phage epsilon15. Phage P19 with genome size of 41 kb was close to phage ST160. And phage P38 with genome size of 48,5 kb was close to phage Gifsy-1.
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