Judul: Comparative Analysis of Food Consumption Patterns in Urban and Rural Adolescents in the Bogor Region
Abstrak: Remaja di perkotaan dan perdesaan diduga memiliki perbedaan praktik konsumsi pangan akibat perbedaan gaya hidup dan faktor-faktor yang memengaruhinya. Kondisi ini bisa membawa pada perbedaan pola konsumsi pangan dan asupan gizi remaja perdesaan dan perkotaan. Penelitian ini bertujuan membandingkan pola konsumsi pangan, gaya hidup, dan akses dalam mendapatkan pangan pada remaja di perkotaan dan perdesaan. Desain penelitian yang digunakan adalah cross sectional. Hasil penelitian menunjukkan bahwa pola konsumsi remaja perkotaan dan perdesaan memiliki beberapa persamaan frekuensi jenis pangan yang dikonsumsi. Gaya hidup berdasarkan variabel frekuensi makan utama, kebiasaan sarapan, frekuensi membawa bekal, dan konsumsi makanan melalui media berbeda signifikan (p<0,05). Variabel susunan menu makan, cara pengolahan makanan, kebiasaan jajan, sumber media informasi fast food, dan bagian iklan tidak berbeda signifikan (p>0,05). Akses sarana dan keterjangkauan pangan berdasarkan variabel asal perolehan bahan pangan pokok mentah dan matang, serta jenis transportasi untuk membeli makanan berbeda signifikan (p<0,05). Variabel ketersediaan makanan dan pengaruh paket promosi fast food tidak berbeda signifikan (p>0,05).
Keyword: adolescents, consumption patterns, lifestyle, rural, urban

Judul: Analisis Hubungan Persepsi Diri dan Makanan, Konsumsi Pangan dan Status Gizi Remaja Usia Sekolah Menengah di Kabupaten Bogor
Abstrak: Adolescent still in process for rich their mental, emotional, social and physical maturity, thinking ability and transition to a new function in social life. One of mentally violence was thinking bad to become fat, that’s way malnutrition happening in adolescent. Negative perception in adolescent was the base of this research. The aim of this research was to analyzed correlation of body images perception, food perception, and food consumption with nutritional status in adolescent. This research used cross-sectional design, done in high schools, which were SMA Negeri 3 Bogor (city) and SMA Negeri 1 Leuwiliang (rural). Primer data collected was from March until June 2012, with 80 student’s second graduate. Data collected by filled the questioner with interview technic, collected family characteristic’s data and individual characteristic’s data. Body image perception’s data got from interviewed and images chose. Food perception’s data got from interviewed. Consumption’s data got from filled food recall 2×24 hours. Nutritional status’s data got from measured in high and weigh. Data was analyzed with Microsoft Excell and Statistical Program for Social Science (SPSS) version 18.0 for Windows. Correlation was analyzed by Pearson correlation and significant at the 0.05 level. SMA Negeri 3 Bogor’s location in the middle of city that has much public facilities and some shopping area in Bogor, had complete facility at school, allowed bringing their laptop and also smart phone. SMA Negeri 1 Leuwiliang far from public facilities and shopping area, located at rural, school has good facility but not same as SMA Negeri 3 Bogor’s facilities and not allowed to bring their gadget. Family size correlated with where they live in and 15% family size from adolescent samples that live at rural is a big family. Father’s income correlated with where they live in, father’s income from adolescent samples that live around the city is more than five million per month (37.5%), occupied as an official employee and had high education. Father’s income from adolescent samples that live around the rural is one to three million per month (47.5%), occupied as a trader and had education until high school’s graduate. Mother’s from adolescent samples 56.3% is a housewife. Mother’s who work, occupied as an official employee (30%) and occupied as a trader (11.3%) for the next. Mother’s education correlated with where they live in and mother’s education from adolescent samples that live around the city 70% was graduates from university and than mother’s education from adolescent samples that live around the rural 35% was graduates from high school. Adolescent samples who live around the city had better food habit than adolescent samples who live at rural (r=0.425, P=0.000). Adolescent samples who live around the city have better nutritional education than adolescent samples who live at rural (r=0.252, P=0.001). x Adolescent samples had a positive perception about their body image (75%). No specific answers from adolescent samples about their food perception and body shape’s ideal concept, so we can say that their food perception is positive. Adolescent samples nutrition adequacy was low, but for energy and protein adequacy was rich to 70% from energy and protein requirement. Average
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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