Judul: Hubungan Antara Kejadian Penyakit Klorosis dan Kerupuk dengan Keberadaan Dua Spesies Kutukebul pada Tanaman Tomat.
Abstrak: Crinivirus and Begomoviruses have been reported to cause serious diseases in a number of economic crops in the world, especially in tomato crop. Crinivirus (Tomato infectious chlorosis virus/TICV and Tomato chlorosis virus/ToCV) causes chlorosis while Begomovirus (Tomato yellow leaf curl virus/TYLCV) causes curl diseases. TICV and ToCV are transmitted by vector insects in semipersistent, but TYLCV in persistent manner. The diseases have been found in Indonesia, especially in West Java. Information about the infecstation of both diseases in tomato crops are still limited. The spread of both diseases on tomato crops based on vector insect abundance in various altitude in Indonesia was evaluated in this study. The study consists of three main activities: (1) a field survey on disease incidence conducted in various altitudes from 100 to 1500 meters above sea level (m asl), which observed variables as follows: (a) diseases incidence, (b) climate condition (temperature and relative humidity (RH)), (c) abundaces of Trialeurodes vaporariorum and Bemisia tabaci, and (d) cropping condition, such as age of plant, varieties, and planting system; (2) the detection and identification of viruses and whiteflies; and (3) the analysis of the relationship between disease incidence and its determinant factors. Minor coat protein gene of Crinivirus (360 bp and 417 bp) and coat protein gene of Begomovirus (780 bp) from symptomed samples in all studied altitudes were successfully amplified by PCR. Some stadia of T. vaporariorum and B. tabaci found during observations had also been identified. The chlorosis and curl diseases infected tomato crops in altitude above 1200 m asl until 1500 m asl. Also, B. tabaci was found at those altitudeswhich previously only found at altitudes below 1200 m asl. The result of regression analysis showed there were positive correlations between the incidence of chlorosis and curl diseases with the presence of the two whitfly spesieses and the increase in altitude. Other factors such as relative humidity, tomato varieties, and plant age did not show significant contributions to the change in the incidence of both diseases.
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Judul: Identifikasi tommato infectious chlorosis virus dan tommato chlorosis virus melalui reverse transcription polymerase chain reaction (RT-PCR) dan sikuen nukleotida
Abstrak: Tomato Infectious Chlorosis Virus (TICV) dan Tomato Chlorosis Virus (ToCV) beberapa tahun ini dilaporkan mulai masuk dan menyerang sejumlah pertanaman tomat di Indonesia. TICV dan ToCV merupakan patogen yang berasal dari genus Crinivirus dan famili Closteroviridae. Penyakit yang ditimbulkan oleh TICV dan ToCV ini mempunyai gejala yang hampir sama di lapangan, yaitu menguning pada bagian interveinal daun, bintik-bintik nekrotik kecil, mengeriting, dan gejala lanjutan akan menyebabkan daun tampak berwarna merah kecoklatan. TICV dapat ditularkan oleh Trialeurodes vaporariorum sedangkan ToCV dapat ditularkan oleh Bemisia tabaci biotipe A dan B, Trialeurodes abutilonea, dan T. vaporarium. Penelitian ini bertujuan untuk mengidentifikasi virus-virus yang berasosiasi dengan penyakit klorosis pada tanaman tomat di Indonesia melalui RT-PCR dan sikuen nukleotida. Metode penelitian ini meliputi ekstraksi RNA virus, sintesis cDNA, amplifikasi DNA, visualisasi hasil RT-PCR, sikuen nukleotida dan analisis filogenetika dengan menggunakan program Bioedit V7.0.5, ClustalW, dan Genetyx versi 7. Berdasarkan hasil RT-PCR menggunakan primer spesifik menunjukkan bahwa amplifikasi TICV menghasilkan panjang pita DNA sebesar 417 bp dan ToCV sebesar 360 bp. Sikuen nukleotida produk RT-PCR tersebut memastikan bahwa penyakit klorosis pada tanaman tomat di Indonesia berasosiasi dengan infeksi TICV dan ToCV. Analisis homologi sikuen nukleotida dan filogenetika menunjukkan bahwa isolat TICV asal Indonesia tergabung dalam satu subsub kelompok dengan isolat TICV asal Jepang dan Spanyol. Adapun untuk isolat ToCV asal Indonesia
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Judul: Analisis tingkat kepuasan pedengar terhadap PT Radio Ika Lesmana
Abstrak: PT. Radio Ika Lesmana merupakan salah satu perusahaan yang bergerak dalam bisnis penyiaran radio. Persaingan yang semakin ketat dalam bisnis penyiaran radio, mewajibkan PT Radio Ika LEsmana agar menempatkan kepuasan pendengar sebagai prioritas utama.
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