Judul: Komposisi Jenis dan Struktur Agroforestri Mindi (Melia azedarach Linn.) di Desa Selaawi Kecamatan Talegong, Kabupaten Garut.
Abstrak: Agroforestri salah satu teknik menggunakan dan mengelola lahan dengan menggabungkan tanaman pertanian dan kehutanan. Mindi merupakan jenis tumbuhan yang cepat tumbuh sangat menyukai cahaya, agak tahan terhadap kekeringan dan toleran terhadap salinitas tanah. Tujuan penelitian ini mengidentifikasi komposisi dan struktur agroforestri mindi pada enam unit lahan dan membandingkan keanekaragaman jenis yang terdapat pada satu unit lahan dengan unit lahan yang lain. Metode pengambilan data di lapang dilakukan dengan membuat plot berukuran 20 m x 100 m yang dibagi ke dalam 5 subpetak berukuran 20 m x 20 m. Hasil penelitian menunjukkan bahwa pola agroforestri yang digunakan beragam dengan komponen penyusun yaitu tanaman pertanian dan tanaman MPTS (Multi Purpose Tree Species). Komposisi jenis yang dihasilkan dari keenam lahan penelitian menghasilkan lahan 4, 5, dan 6 merupakan lahan dengan jumlah jenis yang paling banyak ditemukan dengan jenis yang mendominasi ialah jenis tanaman mindi (Melia azedarach) dan kayu afrika (Maesopsis eminii). Struktur horizontal dengan kerapatan tertinggi dihasilkan pada lahan 6 sebesar 840 individu/ha dengan diameter > 10 cm dan kerapatan terendah pada lahan 3 yaitu 10 individu/ha dengan diameter > 5 cm. Struktur vertikal nilai dari kerapatan semakin menurun dari kelas tinggi > 5 m menuju kelas tinggi pohon > 10 m.
Keyword: agroforestri, dominasi jenis, mindi, tanaman pertanian, tanaman pokok

Judul: Local Knowledge Agroforestry System for Mindi (Melia azedarach L. (Case Study in Selaawi Village, Talegong Subdistrict, Garut, West Java)
Abstrak: Melia azedarach L. is one of the fast growing species which is potential to be developed in community forests. This species is found to occupy most agroforestry lands in Selaawi village (Garut, West Java). Research was conducted in three stand types, namely: (i) pine stand (as reference), (ii) old growth mindi stand, (iii) young growth mindi stand with objectives to: (1) determine site quality of mindi agroforestry, (2) explore the local knowledge on silvicultural techniques of mindi agroforestry, (3) fomulate the strategies for development of mindi agroforestry and determine the genetic and morphology diversity of mindi in Selaawi Vilaage. It was found that site quality in young growth mindi stand was better than that of pine stand and old growth mindi stand. There are several tree species dominating the sites, namely M. azedarach L. (43,37%), Paraserianthes falcataria (23,20%), Maesopsis eminii (15,35%), Manglieta glauca (9.83%), Eucalyptus spp (4,68%), Anthocephalus cadamba (1,62%) and other wood species (1,96%). Local knowledge of mindi agroforestry includes seed procurement, plant propagation, land preparation techniques etc which may be practiced also in other regions. Information obtained from this research could be used to formulate appropriate strategies for sustainable agroforestry management. Microsatelit marker were used to assess the genetic variation. The results showed that the genetic variation of mindi in Selaawi Village was high ranging from He 0,379-0,439.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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