Judul: Hubungan Konsumsi Lemak Dengan Pengetahuan Gizi Serta Status Gizi Anak Usia Sekolah Di Kota Dan Desa Bogor
Abstrak: Tujuan umum penelitian ini adalah mengetahui besar konsumsi lemak pada anak usia sekolah serta hubungannya dengan pengetahuan gizi dan status gizi anak usia sekolah di kota dan desa, sedangkan tujuan khususnya adalah mengetahui tingkat pengetahuan gizi anak usia sekolah, mengetahui kebiasaan makan anak usia sekolah, mengetahui besar kontribusi lemak terhadap total konsumsi energi anak usia sekolah, mengetahui komposisi lemak menurut kelompok pangan yang dikonsumsi oleh anak usia sekolah
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Judul: Konsumsi Lemak Dan Status Gizi Remaja di Bogor
Abstrak: Tujuan urnurn penelitian ini adalah untuk mengetahui konsumsi lemak dan kaitannya dengan faktor-faktor yang mempengaruhinya serta kaitan konsumsi lemak dengan status gizi remaja di kota dm desa. Secara khusus tujuan penelitian ini adalah; 1) mengetahui keadaan sosial ekonomi keluarga yang rneliputi pendidikan orang tua, pekerjaan orang tua, pendapatan orang tua, dan besarnya uang saku remaja; 2) mengetahui pengetahuan gizi remaja di kota dan desa; 3) mengetahui kebiasaan rnakan remaja di kota dan desa; 4) mengetabui komposisi lemak rner.urut jenis makanan surnber lemak dan jenis lemak yang dikonsumsi ~cmajad i kota dan desa; 5) mengetahui besar kontribusi lemak terhadap total konsumsi energi remaja di kota dan desa; 6) mengetahui kaitan konsumsi lemak dengan faktor-faktor ymg mernpengaruhinya serta kaitan antara konsumsi lemak dengan status gizi .
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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