Judul: Optimization High Performance Liquid Chromatographic Fingerprint of sappan wood (Caesalpinia sappan)
Abstrak: Mixture design has been applied for optimization of Caesalpinia sappan chromatographic fingerprint. The design applied for unreplicated and simultaneous optimization of HPLC mobile phase mixture. Ethanol extracts with highest antioxidant activity resulted from sonication extraction method was chosen for HPLC analysis . The reversed phase chromatographic mobile phase in simplex centroid design consist of varying proportion of methanol 50 %, methanol: water: TFA (25: 75: 0.025 v/v), and methanol: water: TFA (15: 85: 0.035 v/v). Ethanol 70 % extract of sappan analyzed with ten mobile phase and monitored at 254 and 280 nm. Correlation between HPLC mobile phase and a number of peak analyzed statistically by SAS 9.2. The root mean square errror of calibration (RSMEC) and root mean square errror of calibration (RSMEP) at 254 and 280 nm, were 8.3006 and 8.29659, 3.88730 and 4.85193 respectively. Optimum condition obbtained when ethanol 70 % extract eluted by methanol: water: TFA (15: 85: 0.035 v/v), with the 15 number of peaks.
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Judul: Pengoptimuman dan Validasi Sidik Jari Kromatografi Cair Kinerja Tinggi Ekstrak Phyllanthus niruri L.
Abstrak: Split-plot mixture-mixture design has been applied for optimization of Phyllanthus niruri L. chromatographic fingerprint. The design applied for unreplicated and simultaneous optimization of extraction mixture and chromatographic mobile phase mixture. The whole-plot was extraction solvent contained varying proportion of methanol, ethyl acetate and dichloromethane in a simplex-centroid with axial design. The sub-plot was reversed phase chromatographic mobile phase in simplex-centroid design contained varying proportion of methanol, acetonitrile and acetonitrile:water (55:45 v/v). Each extract analyzed with seven mobile phase and monitored at 210, 225 and 254 nm. Ratio whole plot error to subplot error smaller than 0,4 (/ < 0,4). Correlation between extraction solvent, chromatographic mobile phase and number of peak analyzed statistically by Ordinary Least Square (OLS) method. The root mean square error of calibration (RMSEC) and root mean square error of prediction (RMSEP) at 210, 225 and 254 nm respectively were 1,86341 and 4,00759; 2,22201 and 5,28394; 1,54367 and 2,26063. Optimum codition obtained when ethyl acetate extract eluted by acetonitrile:water (55:45 v/v) and monitored at 254 nm. Validation of optimum condition performed for precision and extract stability test parameter. Precision of retention time at optimum condition was excellent, percent relative standard deviation (%RSD) were 0,0698 % - 0,3006 %. Extract stability test examined after 2,5 and 5 hours storage at 25 °C with protection from light, peak area of each extract component changed in different level during the storage.
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Judul: Infeksi Virus Parainfluenza Pada Anjing
Abstrak: Virus parainfluenza anjing termasuk famili Paramyxoviridae, berbentuk pleomorf dengan ukuran bervariasi (bergaris tengah antara 100-500 nm), berpembungkus peka ether, berisi genom RNA dan secara serologis mempunyai kesamaan dengan virus SV-5 kera; dikenak sebagai salah satu kausa penting dari sindrom "kennel cough". Infeksinya dapat mengenai anjing semua umur yang belum pernah terinfeksi atau mendapatkan vaksinasi mengakibatkan gejala klinis ringan yang terbatas pada traktus respirasi atu infeksi yang secara klinis tidak memperlihatkan gejala nyata. Gejala klinis yang nampak setelah masa inkubasi (1-5 hari) terlewati berupa batuk yang tidak berat selama beberapa hari, berbagai tingkat tonsilitis dan pharyngitis dan kadang-kadang ditemukan kenaikan temperatur rektal antara 1-2 derajat C dari temperatur normal rat-rata. Anjing terinfeksi mengandung virus pada traktus respirasi bagian atas dan bawah serta limfonoduli sampai hari ke 9 setelah infeksi. Penyebaran virus antar anjing terjadi secara aerogen.
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