Judul: Desain Lanskap Agrowisata Tamansari Berbasis Kearifan Lokal Budaya Sunda di Kabupaten Bogor
Abstrak: Economic development is coherent with industrialization in the cities. It also decrease social values and local culture indirectly. One effort to solve this problems is to conserve agriculture and local wisdom itself. Agrotourism is one of tourism type which use agriculture as its object. Tamansari site, Bogor, has potencies to become agrotourism because of authentic agroecosystem and suitable soil for agriculture. This research is purposed to design agrotourism based on Sundanese local wisdom. The methods is spatial analysis to process physical and biophysical data, quantitative analysis to quantify Carrying Capacity, Thermal Humidity Index (THI), and Agrotourism Area Feasibility Assignment, and also descriptive analysis to know user preferences. The synthesis is agrotourism development area and Opat Kalima Pancer concept design implementation. This research output is design drawing (siteplan) completed with detail drawings (section plan, perspective drawing, and planting plan).
Keyword: Bogor Agricultural University (IPB), Sundanese Culture, Local Wisdom, Agrotourism, Landscape Design

Judul: Desain Lanskap Kawasan Pertanian Penginapan Tjikoepa Berbasis Budaya Sunda
Abstrak: Modernisasi dan globalisasi telah mengakibatkan minat masyarakat pada nilai-nilai budaya Sunda mengalami penurunan. Hal ini menyebabkan budaya Sunda sudah mulai ditinggalkan. Apabila dibiarkan tanpa adanya upaya pelestarian yang konkret maka budaya Sunda terancam mengalami kepunahan. Pola ruang dan arsitektur tradisional Sunda dapat digunakan sebagai referensi untuk desain lanskap dan ruang terbuka hijau guna meningkatkan identitas lokal Sunda dan mengenalkan budaya Sunda. Oleh karena itu, diperlukan penelitian mengenai pola ruang dan lanskap tradisional Sunda sebagai bentuk upaya pelestarian terhadap nilai-nilai budaya Sunda. Penelitian ini bertujuan untuk mendesain kawasan pertanian Penginapan Tjikoepa berbasis budaya Sunda. Metode yang digunakan dalam penelitian ini adalah metode deskriptif kualitatif melalui observasi lapangan, studi literatur, dan wawancara. Pendekatan desain yang digunakan adalah activity-based design. Ruang-ruang yang direncanakan meliputi ruang penerimaan, ruang pelayanan, ruang pribadi pemilik, ruang display, ruang komunal, ruang peternakan, perkebunan alpukat, perkebunan durian, perkebunan kopi, perkebunan hijauan, ruang offroad, ruang equestrian, ruang glamping, dan ruang relaksasi. Konsep dasar yang diterapkan adalah lanskap rekreasi berbudaya Sunda. Konsep desain yang diangkat adalah konsep kaca-kaca. Nilai-nilai budaya Sunda diterapkan pada tapak untuk melestarikan dan memperkenalkan budaya Sunda kepada masyarakat. Pergerakan didesain untuk memberi pengalaman utuh dan menyeluruh melalui ruang-ruang tersebut. Puncak pergerakan berada pada point of view dengan pemandangan bukit-bukit yang indah. Keterpaduan sistem pertanian melibatkan siklus komoditas, produk, dan limbah. Pengunjung disuguhkan karakter budaya Sunda yang tercermin mulai dari gerbang masuk sampai point of view., Modernization and globalization have caused people's interest in Sundanese cultural values to decline. This has caused Sundanese culture to begin to be abandoned. If left without concrete preservation efforts, Sundanese culture is threatened with extinction. Traditional Sundanese spatial and architectural patterns can be used as references for landscape design and green open spaces to enhance local Sundanese identity and introduce Sundanese culture. Therefore, research is needed on traditional Sundanese spatial and landscape patterns as a form of effort to preserve Sundanese cultural values. This research aims to design the Tjikoepa Inn agricultural area based on Sundanese culture. The method used in this research is a qualitative descriptive method through field observations, literature studies, and interviews. The design approach used is activity-based design. The planned spaces include a reception space, service space, owner's private space, display space, communal space, livestock space, avocado plantation, durian plantation, coffee plantation, forage plantation, offroad space, equestrian space, glamping space, and relaxation space. The basic concept applied is a Sundanese cultural recreation landscape. The design concept raised is the kaca-kaca concept. Sundanese cultural values are applied to the site to preserve and introduce Sundanese culture to the community. Movement is designed to provide a complete and comprehensive experience through these spaces. The peak of the movement is at a point of view with beautiful views of the hills. The integration of agricultural systems involves commodity, product, and waste cycles. Visitors are presented with the character of Sundanese culture which is reflected from the entrance gate to the point of view.
Keyword: cultural preservation, cultural tourism, nature tourism, agrotourism, integrated farming

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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