Judul: Analisis Hubungan Curah Hujan dengan Volume Genangan Air di Perumahan Tamansari Persada, Bogor
Abstrak: Negative impact due to the disappearence of water catchment is the decrease of land capability to infiltrate rainwater causing run off, ponding water or even flooding. This research was conducted with anobjective to know a relationship between rainfall and volume of the ponding water. The location selected in this research was Tamansari Persada Residence in Bogor between March until April 2013. The research was conducted in two phases, which were primary data collection by measuring coordinate points of the ponding water and secondary data collection of daily rainfall. Gridding method based on Surfer 8 was used to calculate the volume. The results showed linear equation relationships between rainfall and volume of ponding water at Location 1 is y = 14.80x - 1.456 with the coefficient of determination 0.558, in Location 2 is y = 30.56x + 96.18, with the coefficient of determination 0.901, and Location 3 linear is y = 53.16x + 46.89, with the coefficient of determination 0.685. The small coefficient of determinations at Locations 1 and Location 3 might be caused by several factors such as ineffective use of time measurement.
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Judul: Penentuan Lokasi, Luas, dan Volume Genangan Air Sebagai Potensi Cadangan Airtanah di Perumahan Tamansari Persada, Bogor
Abstrak: The changes of the green land-use as retention area to become housing area, will cause problems like enhancement of run-off, reduction of water infiltration to the land, as well as puddle and flood if water volume increases in some area. A study was conducted with the objective to determine the location, area, and volume of puddles at Tamansari Persada Residential, Bogor as groundwaters storages potential. The procedure of sampling and analysis of the data were determination of fixed location of puddles, determination of coordinate points for area and volume of puddles, and analysis of the data by aSurfer 8’s software and Gridding Krigging Method. According the research result from March to April 2013, there were three fixed puddles locations at Palm Cluster and routine flood occured when rain was falling and the maximum volume and area puddles for the first location were 0.11 m3 and 6.57 m2, the second location were 0.26 m3 and 18.82 m2, and the third location were 0.35 m3 and 20.77 m2. Generally, there are correlation from enhancement of lowest elevation, areas, and volumes of puddle and have been proved by Linear Regresion Method. From this method will produced puddle potential formula and the value of determination coefficient (R2) closer to 1. The location, areas, and volume of puddles could becoming reference of puddles potential and absorbed to the ground as an groundwater.
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Judul: Primer Spesifik Ikan Hiu Biru (Prionace glauca) Berbasis Marka Cytochrome B untuk Autentikasi Produk Olahan Ikan Hiu
Abstrak: Primer merupakan aspek krusial yang perlu diperhatikan dalam proses mengidentifikasi spesies pada produk olahan hiu berbasis genetik. Jenis marka mtDNA yang dapat digunakan untuk mengidentifikasi species salah satunya adalah gen cytochrome b. Prionace glauca merupakan spesies ikan hiu yang tersebar luas di perairan Indonesia yang jumlah populasinya terus mengalami penurunan. Mendesain primer cytochrome b spesifik Prionace glauca menjadi tujuan dalam penelitian untuk mengidentifikasi adanya spesies tersebut dalam produk olahan ikan hiu komersil. Primer didesain pada fitur Primer-BLAST pada situs NCBI. Primer diujikan melalui proses amplifikasi pada isolat DNA produk olahan hiu yang meliputi ikan hiu asin (A1), abon ikan hiu (B1 dan B2), ikan hiu asap (C1), ikan hiu empal (E1), dan fillet ikan hiu (F1 dan F2) dengan tingkat kemurnian 0.909-2.106 (A260/280). Pada suhu annealing 55oC teridentifikasi ukuran DNA seluruh sampel yang diujia dalah 500 bp. Sekuens dari sampel yang telah diamplifikasi menunjukkan hasil BLAST spesies yaitu Prionace glauca dengan tingkat kemiripan mencapai 99,66%. Kedekatan genetik pada hasil filogenetik terlihat pada kelompok yang terbentuk dengan spesies target Prionace glauca dengan nilai bootstrap 100%., Primer is an essential aspect of the species identification procedure. One of the DNA sourced at cytochrome B mtDNA is recognized suitable as a marker. Shark species that distribute almost in every Indonesia's sea is Prionace glauca. A pair of specific cytochrome b primers were designed with the Primer-BLAST feature on NCBI. The primer got applied at DNA amplification that run to analyze some products labeled as salted shark meat (A1), shark meat floss (B1 and B2), smoked shark meat (C1), shark empal (E1), and shark meat fillet (F1 and F2), have 0,909-2,106 (A260/280) purity rate. The sizes of all DNA samples were identified at 500 bp at 55oC annealing temperature. Prionace glauca was spotted at BLAST result from the sample's sequence with the percent identity up to 99,66%. The phylogenic tree shows a group containing product and Prionace glauca with 100% bootstrap value.
Keyword: Prionace glauca, Cytochrome b, Ikan hiu, Primer spesifik