Judul: Optimasi Aktivitas Selulase Ekstraseluler dari Isolat Bakteri RF-10
Abstrak: Cellulase is one of the enzymes used in many industrial applications. High enzyme price is the major problems faced in enzyme based industrial. One of the solution to decrease the enzyme price is to explore the potential microbe as producers of cellulase. This study aimed to determine the optimum incubation time for cellulase production of the RF-10 bacterial isolates and optimized optimum temperature and pH of cellulase. Cellulase activity of RF-10 bacterial isolate qualitatively was measured by Congo Red 0.1% staining method and quantitatively by 3.5-dinitrosalicylic acid method. The results showed that RF-10 produced cellulase based on clear zone formation and extracellular enzymes was obtained 1.625. The maximum cellulases production in CMC medium reached at the 42 hours with the activity 10.712 x 10-6 U/mL. Optimization of enzyme activity showed that the optimum temperature at 50 °C and optimum pH at 6.5. In addition, the enzyme has actively working in the range of pH 5.5-7.5.
Keyword: 

Judul: Isolasi dan Identifikasi Bakteri Termofilik Penghasil Selulase dari Kelapa Sawit (Elaeis guineensis)
Abstrak: Cellulase is an enzyme catalyzing hydrolysis of cellulose which usually consists of endoglucanases (EC.3.2.1.4), exoglucanases (EC.3.2.1.91) and β-glucosidases (EC.3.2.1.21). Cellulase is a very important enzyme due to its numerous industrial applications. The aim of this research were to isolate and identify cellulase-producing thermophilic bacteria that can be used to increase value added in oil palm industries. Samples of soil, empty fruit bunch compost and palm kernel meal were collected from oil palm plantation for obtained thermophilic bacteria. The samples were screened of cellulase-producing thermophilic bacteria by using a Congo Red method were made on carboxymethyl cellulose (CMC) agar plates. The bacterial cultures were incubated in a shaking incubator (140 rpm) at 50oC for 24 hours. The assay for the enzymatic activity was based on the release of glucose that was detected using 3,5-dinitrosalicylic acid (DNS). In this research, 19 isolates of bacteria were isolated. It was found that 11 isolates of bacteria showed positive results with clear zone around the cultures by using Congo Red method. The result showed that isolates for CK1, EM4 and CK3 possesed the highest enzyme activity of 13.56, 12.50 and 10.49 U mL-1 for cellulase was detected at pH 7, respectively. The results of strain identification based on 16S rRNA showed that strain CK1, EM4 and CK3 were identified as Bacillus subtilis.
Keyword: 

Judul: Analisa Prestasi Kuda Pacu Pada Kejuaraan Nasional Pacuan Derby Indonesia Tahun 1974-2015
Abstrak: Olahraga berkuda memerlukan ternak kuda yang bermutu tinggi dan terlatih dengan baik. Oleh karena itu, organisasi nasional bernama PORDASI (Persatuan Olah Raga Berkuda Indonesia) melakukan program grading up kudakuda lokal Indonesia. Program grading up telah dilakukan selama 41 tahun yang lalu sampai sekarang dan perlu dilakukan ulasan atas program tersebut. Tujuan dari penelitian ini adalah untuk menganalisis prestasi kuda pacu Indonesia pada kejuaraan nasional derby Indonesia tahun 1974-2015. Data yang digunakan merupakan data sekunder dari 111 ekor kuda yang menempati posisi 1, 2, dan 3. Penelitian ini menggunakan metode deskriptif. Kuda Generasi ke-4 (G4) memiliki prestasi paling banyak dibanding kuda peranakan Thoroughbred lainnya. Kuda ini mendominasi pacuan pada jarak 2 000 meter. Asal tetua pejantan dari Thoroughbred Australia dan betina lokal priangan paling banyak menghasilkan kuda juara pacuan Derby Indonesia. Persentase jenis kelamin jantan dan betina untuk posisi finish ke 1,2, dan 3 tidak terlalu berpengaruh pada prestasi kuda. Kuda dengan persentase darah Thoroughbred lebih tinggi berpeluang untuk berprestasi pada jarak pacu yang lebih jauh.
Keyword: grading up, jarak pacu, jenis kelamin, prestasi, Thoroughbred