Judul: Kajian Etnobotani Suku Buton (Kasus Masyarakat Sekitar Hutan Lambusango, Kabupaten Buton, Provinsi Sulawesi Tenggara)
Abstrak: Hutan Lambusango merupakan salah satu ekosistem hutan hujan dataran rendah yang terdapat di Pulau Buton. Hutan Lambusango merupakan satu kesatuan ekosistem hutan yang terdiri dari Suaka Margasatwa Lambusango (27.700 ha), Cagar Alam Kakinauwe (810 ha) yang di sekitarnya terdapat hutan produksi (35.000 ha). Masyarakat suku Buton di sekitar hutan Lambusango memiliki kearifan tradisional dalam memanfaatkan tumbuhan untuk kehidupan sehari-hari serta memiliki peran serta dalam kegiatan konservasi tumbuhan berguna.
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Judul: Populasi dan Pemanfaatan Katak Limnonectes grunniens di Pulau Buton, Sulawesi Tenggara
Abstrak: Katak mempunyai peranan yang penting secara ekologi maupun ekonomi. Secara ekologi katak merupakan komponen dalam rantai makanan. Katak memiliki nilai ekonomi sebagai komoditas perdagangan. Indonesia merupakan salah satu negara pengekspor paha katak ke benua Eropa, Amerika, dan Asia. Salah satu jenis katak yang masuk dalam kuota ekspor adalah Limnonectes grunniens. Jenis tersebut dilaporkan mengalami penurunan populasi di alam, terutama karena degradasi habitat dan konsumsi yang dilakukan manusia. Hutan Lambusango yang terletak di Pulau Buton, Sulawesi Tenggara merupakan habitat bagi katak L. grunniens. Namun jumlah populasi, ekologi, biologi, dan pola pemanfaatan katak tersebut belum diketahui. Penelitian bertujuan untuk mengidentifikasi karakteristik dan kondisi habitat, menganalisa karakteristik morfologi dan mengestimasi populasi, menganalisa perilaku yang meliputi strategi aktivitas dan tingkat kewaspadaan terhadap predator, menganalisa pola pemanfaatan katak L. grunniens oleh masyarakat yang tinggal di sekitar kawasan Hutan Lambusango, Pulau Buton, Sulawesi Tenggara
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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