Judul: Cloning Method of Pest Resistance Gene from Sengon (Paraserianthes falcataria).
Abstrak: Sengon is one of fast growing trees species, which is widely cultivated in plantations forest in Indonesia due to its multipurpose nature, and easy to grow. The plantation, however, faces serious problem of stem borer (Xystrocera festiva) attack, which was reported could destroyed sengon plantation. Meanwhile, several studies have reported the role of trypsin inhibitors (TI) and alpha-amylase inhibitor (AAI) in resistance in trees to insects. This research aimed at cloning a possible pest resistance gene from sengon. Sengon genomic DNA was isolated using CTAB buffer combined with binding columnn of GenElute plant Genomic DNA Miniprep Kit. Designed primer pairs did not generate amplicon. PCR products were obtained from published degenerate primers as two amplicons, with predicted size of 350 bp and 550 bp for AAI gene, and 600 bp and 800 bp for TI gene. Cloning with pGEM-T Easy vector resulted in several white colonies. Sequencing from selective colonies with long and short amplicon of AAI and TI sequences with 693 nucleotide, 278 nucleotide and 832 nucleotide, 278 nucleotide, respectively. BLASTN and BLASTX the sequences did not confirm that the inserts were AAI and TI genes. Further research is still needed to identify pest resistance gene in sengon tree.
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Judul: Optimasi Kloning dan Identifikasi Gen Penyandi Tripsin Inhibitor dan -Amilase Inhibitor pada Tanaman Sengon (Falcataria moluccana Miq.)
Abstrak: Sengon (Falcataria moluccana) adalah tanaman cepat tumbuh yang banyak digunakan terutama pada hutan rakyat. Namun, peningkatan tren penggunaan sengon menyebabkan ledakan hama, salah satunya adalah penggerek batang boktor (Xystrocera festiva). Larva boktor menghasilkan enzim -amilase dan tripsin dalam sistem pencernaannya untuk mencerna kulit dan kayu gubal sengon. Beberapa aksesi sengon resisten menunjukkan aktivitas inhibitor tehadap enzim -amilase dan tripsin yang merupakan aspek penting dari program pemuliaan pohon sengon. Pendeteksian gen ketahanan dapat dilakukan dengan pembuatan pustaka genomik melalui metode shotgun cloning dan kloning gen spesifik. Tujuan penelitian ini adalah untuk mengoptimasi penyiapan bahan kloning dan metode pembuatan pustaka genom serta kloning gen tripsin inhibitor dan -amilase inhibitor pada sengon. Sekuen gen tripsin inhibitor sengon didapatkan berukuran dari 107 bp hingga 308 bp dan memiliki kesamaan dengan Tamarindus indica, Glycine max, Populus trichocarpa dan Populus nigra. Sekuens gen -amilase inhibitor didapatkan berukuran 447 bp dan memiliki kesamaan dengan Phaseolus vulgaris. Namun, sekuens TI dan AAI tersebut masih memiliki nilai E yang rendah.
Keyword: tripsin inhibitor, -amilase inhibitor, F.moluccana, X. festiva

Judul: Persamaan Korteweg-de Vries Orde Tinggi untuk Menggambarkan Gerak Gelombang Internal
Abstrak: The ocean can be considered of layers of fluid that each layer has a constant density. Internal waves are waves occur in the interface of every'two layers with different density. Because it occures underneath the surface of the ocean, it is invisible. One of internal waves is internal solitary waves. One of formulation that can describe intemal waves movement is KdV equation. KdV equation is derived from governing equations of ideal fluid in Lagrange formulation using asymptotic method. On this thesis, KdV equation is derived in higher order and then its solution is derived by using internal solitary waves assumption. An example of a two-layer fluid is a case will be used. By using Mathematica6 software, for this case will be obtained the coefficients in lower and higher order explicitly and the corresponding between coefficients and relative lower layer depths and fluid depths, the corresponding between coefficients and relative densities, and also observed the different between solitary waves in lower and higher order.
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