Judul: The Relationship Between Nutritional Knowledge and Food Consumption Patterns and the Nutritional Status of Al-Ukhuwah Islamic Kindergarten Teachers in Tangerang
Abstrak: A teacher's health plays an important role in the teaching and learning process. Various kinds of nutritional problems in teachers or adults can occur, one of which is obesity. This research uses a cross-sectional study design. The subjects in this research were 26 Islamic Kindergarten teachers A;-Ukhuwah Tangerang. Subjects were drawn using purposive sampling. Data on the subject's food consumption patterns was determined using the Food Frequency Questionnaire (FFQ) and the level of nutritional adequacy, amount and type of food consumed using 2x24 hour food recall. For data processing and analysis, Microsoft Excel 2013 and the SPSS 16.0 for Windows were used. Energy adequacy is 30.8%, moderate deficit, excess protein and fat is 34.6% and 50%. Twelve of the 26 subjects were declared to be severely obese. There was no correlation between food consumption patterns (staple food (r=0.138), side dishes (r=0.363), vegetables (r=0.319), and fruit (r=0.355)) with the subject's nutritional status. The level of nutritional adequacy is also not related to nutritional status. This can be seen from the p-value of each level of sufficiency, namely energy (p=0.358), protein (p=0.627), fat (p=0.985) and carbohydrates (p=0.993). Keywords: Nutritional status, nutritional knowledge, food consumption pattern
Keyword: status gizi

Judul: Correlation between Knowledge, Attitudes, Nutrition Practices, and Food Consumption with The Nutritional Status of Primary School-aged Children in Bogor City
Abstrak: Anak usia sekolah dasar membutuhkan asupan gizi yang baik sesuai dengan kebutuhannya. Permasalahan gizi pada anak dipengaruhi oleh kebiasaan makan dan aktivitas fisik anak. Tujuan penelitian ini adalah menganalisis hubungan pengetahuan, sikap, praktik gizi dan konsumsi pangan dengan status gizi anak usia sekolah dasar di Kota Bogor. Desain penelitian ini adalah cross-sectional yang dilaksanakan pada bulan September 2022-Januari 2023 di Kota Bogor. Subjek penelitian berusia 9-12 tahun dengan status gizi lebih sebesar 20% dan obesitas sebesar 12,5%. Pengambilan data dilakukan dengan pengisian kuesioner serta wawancara food recal 2x24 jam, recall aktivitas fisik 2x24 jam dan Food Frequency Questioner (FFQ). Tidak terdapat hubungan yang signifikan antara pengetahuan (p=0,313), sikap (p=0,172), praktik gizi (p=0,351), tingkat kecukupan energi (p=0,960), tingkat kecukupan protein (p=0,533), tingkat kecukupan lemak (p=0,785), tingkat kecukupan karbohidrat (p=0,555) dan aktivitas fisik (p=0,501) dengan status gizi., Primary school-age children need good nutritional intake according to their needs. Nutritional problems in children are influenced by eating habits and physical activity. The purpose of this study was to analyze the correlation between knowledge, attitudes, nutritional practices and food consumption with the nutritional status of primary school-age children in Bogor City. The design of this study was cross-sectional which was carried out in September 2022-January 2023 in Bogor City. The research subjects were aged 9-12 years with excess nutritional status of 20% and obesity of 12.5%. Data collection was carried out by filling out questionnaires as well as 2x24 hour food recall interviews, 2x24 hour physical activity recall and Food Frequency366 Questioner (FFQ). There was no significant relationship between knowledge (p=0.313), attitude (p=0.172), nutritional practices (p=0.351), energy adequacy level (p=0.960), protein adequacy level (p=0.533), fat adequacy level (p=0.785), level of carbohydrate adequacy (p=0.555) and physical activity (p=0.501) with nutritional status.
Keyword: attitudes, Food consumption, knowledge, nutritional status, nutritional practices

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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