Judul: Analysis Factors That Influence the Success of Perpustakaan Elektronik Keliling (Pusteling) Using an Analytic Hierarchy Process (AHP) Method)
Abstrak: The research object is Perpustakaan Elektronik Keliling (Pusteling) or Electronic Mobile Library service, that is a mobile library service operated by National Library of Indonesia. Pusteling is a mobile library bus aimed to introduce and promote the utilization of information technology and communication to public especially among students. The goals are to determine the factors that affect the Pusteling service’s success and failures (if any) and how far those factors influenced it. The factors identified by internal and external factors. The data that are collected from the questionnaires were processed using an Analytic Hierarchy Process (AHP) method. Based on these results, from the four priority criterias that affect Pusteling, the first results Institution has the highest percentage (29.3%) of the global weight, the second, third and fourth are Social criteria (29.2%), Pusteling Technology (24.8%) and Region criteria (16.7%). Its indicated the institutional component must be supported by a good social and technology to revamping Pusteling, with that Pusteling region operational can develop more widely with the result that users were more increased.
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Judul: Pemodelan Analytical Hierarchy Process (AHP) Untuk Penentuan Faktor yang Berpengaruh Pada Pengguna Website Perpustakaan
Abstrak: Library websites are communication tools that can facilitate users to interact with the need for information. This research was conducted to determine the factors that influence the users library wbsites by using modeling methods Analitycals Hierarchy Process (AHP). The purpose of the research is to analyze which factors that influence the user of library websites. As the respondents, the students of five faculties in Institut Pertanian Bogor were taken as a sample. The result shows that the factors influence the user’s library websites are the enjoyment as the highest value 0.543, then empathy 0.522, and the response time 0.522. This condition indicates that the site managers are necessary to consider the needs of the users, especially system quality as relative importance of each website quality and priority of alternative subject.
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Judul: Gene Editing  of Two Cyanide Synthesis-related Genes in Cassava using CRISPR/Cas9
Abstrak: Cassava (Manihot esculenta) contain toxic quantities of cyanogenic glucoside. Reducing the potentially toxic cyanogenic glucoside levels in cassava could be achieved by gene editing through Clustered Regularly Interspaced Short Palindromic Repeats – CRISPR associated protein 9 (CRISPR/Cas9), a high- precision genome editing technique in plants. Two cassava UDP- glucosyltransferase (UGT) paralogs, UGT85K4 and UGT85K5, are the genes catalyzing the synthesis of cyanogenic glucosides in plants. The study aimed to construct the CRISPR/Cas9 recombinant vector carrying single guide RNA (sgRNA) to edit UGT85K4 and UGT85K5 genes, and transform sgRNA CRISPR/Cas9 construct into KU50 cassava calli. Five sgRNAs were designed, considering the on/off target efficiency score and GC content. Those sgRNAs were ligated into pRGEB32 plasmid and introduced to competent Escherichia coli DH5a. The PCR colony and DNA sequencing were performed to verify the sgRNA insert. The amplicons showed the expected band size of recombinant vector (180 bp). Based on sequencing results, only one sgRNA was successfully ligated into the plasmid that will be used to edit the UGT85K5 gene. The sgRNA CRISPR/Cas9 construct in pRGEB32 plasmid was successfully transformed into Agrobacterium tumefaciens and introduced into KU50 cassava calli through co-cultivation. The overgrowth of Agrobacterium occurred after the co-cultivation phase.
Keyword: CRISPR/Cas9, Cyanogenic glucoside, Gene editing, sgRNA