Judul: Struktur Komunitas di Perairan Pantai Batu Namprak, Sukabumi, Jawa Barat
Abstrak: Penelitian ini bertujuan untuk mempelajari struktur komunits rumput laut pada daerah intertidal Batu Namprak Sukabumi, Jawa Barat yang meliputi komposisi jenis, penutupan dan kepadatan serta kaitannya dengan faktor-faktor lingkungan yang mempengaruhinya. Penelitian dilakukan selama 2 bulan. Pengamatan dilakukan pada pagi dan siang hari saat surut melalui observasi visual "in situ" dan pengambilan contoh menurut garis transek dan plot kuadrat (50x50 cm). garis transek ditentukan tegak lurus pantai ke arah tubir. Pada setiap lima meter sepanjang garis transek tersebut diambil sampel rumput laut pada are plot (50x50 cm), selanjutnya dicatat pula penutupan rumput laut, subtrat dasar serta kedalaman air. Pemeriksaan sampel dilakukan dengan cara sortasi berdasarkan marga dan penimbangan berat (bobot) basah
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Judul: Tinjauan Beberapa Aspek Ekologis Rumput Laut Di Pantai Batu Namprak Dan Kelapa Condong Kecamatab Ciracap, Kabupaten Sukabumi Jawa Barat
Abstrak: Penelitian ini dilakukan pada bulan April sampai bu lan Mei 1985, bertujuan menginveritasi jenis-jenis rumput laut (seaweed dan seagrass) yang terdapat di kedua pantai penelitian, yaitu di pantai Batu Namprak dan di Kelapa Condong serta mengetahui kepadatan, keragaman dan keseragaman, kemiripan antar komunitas, hubungan antar jenis dan penyebarannya. Pengamatan dilakukan pada saat air surut, yaitu pada pagi, siang dan sore hari di dua stasiun, dengan menempatkan bingkai kuadrat (1x1) m² pada setiap titik 2 plot. Banyaknya plot (satuan contoh) pada tiap-tiap sub stasiun tidak seragam, tergantung jarak dari tubir ke arah pantai. Selain itu dilakukan pula pengukuran kuali- tas perairan sebagai data penunjang, meliputi suhu, pH dan salinitas serta dilakukan pengamatan terhadap substrat dasar sebagai habitat rumput laut. Faktor substrat sangat berpengaruh terhadap keberadaan rumput laut di perairan, baik kepadatan, keragaman, keseragaman, asosiasi antar jenis maupun penyebarannya.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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