Judul: Diferensiasi beberapa Isolat Rice Tungro Virus dengan Kultivar Padi Diferensial dan PCR - RFLP
Abstrak: Tungro merupakan penyakit tanamna padi yang disebabkan oleh infekksi dua macam virus, yaitu rice tungro bacilliform badnavirus (RTBV) and rice tungro spherical waikavirus (RTSV). RTBV menyebabkan gejala tungro meliputi perubahan warna daun kuning sampai oranye dan tanaman menjadi kerdil. RTSV sendiri tidak menyebabkan gejala yang jelas, hanya kerdil ringan, tetapi memperparah gejal yang disebabkan oleh RTBV. Tujuan penelitian ini adalah untuk membedakan isolat virus tungro dari beberapa daerah endemik tungro di Indonesia dengan tanaman diferensial dan PCR-RFLP. Delapan isolat virus tungro dikumpulkan dari beberapa daerah endemik tungro di Indonesia, yaitu: Bali, Kalimantan Selatan, Sulawesi Selatan, Jawa Barat (2 isolat), Jawa Tengah, Jawa Timur, dan Nusa Tenggara Barat.
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Judul: Keragaman Gen Protein Selubung Rice tungro bacilliform badnavirus pada Suatu Luasan Hamparan Padi di Desa Ciherang, Kecamatan Darmaga, Kabupaten Bogor.
Abstrak: Tungro merupakan salah satu penyakit tanaman padi terpenting di negara-negara Asia Selatan dan Asia Tenggara yang terutama ditularkan oleh Nephotettix virescens Distant (Hemiptera: Cicadellidae). Penyakit tungro disebabkan oleh infeksi kompleks dua virus, RTBV (Rice tungro bacilliform badnavirus) dan RTSV (Rice tungro spherical waikavirus). Beberapa isolat RTBV dilaporkan mempunyai keragaman genetika pada gen protein selubung RTBV yang beragam. Penelitian ini bertujuan untuk mengetahui keragaman gen protein selubung RTBV pada suatu luasan hamparan tanaman padi di Desa Ciherang, Kecamatan Darmaga, Kabupaten Bogor.
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Judul: Synthesis of Apatite - Chitosan Composite Using Duck Eggshell and Chitosan with Temperature and pH Control
Abstrak: Composite of apatite-chitosan was prepared by using chitosan as polymer matrix. Calcium phosphate was prepared from CaO and (NH4)2HPO4, which the ratio Ca : P had to be 1.67. Chitosan is produced by deacetilase from biopolymer chitin. Duck eggshell (95% CaCO3) as starting material was converted to be calcium oxide (CaO) through calcinations with temperature 1000oC for 5 hours. In-situ and ex-situ methods were prepared from apatite and chitosan (dissolved with acetate acid 2%). Calcium phosphate prepared for bone implantation, but it is too fragile and brittle to be implant into the body. So addition of chitosan can decrease crystalinity and increase mechanic characteristic (tougher). Composite apatite-chitosan was resulted by fast precipitation. Fast precipitation was held in a breaker glass with nitrogen atmosphere, under controlled temperature at 37oC and pH 7, in order to increase amount of hydroxyapatite. Precipitation with CaO which was dropped by (NH4)2HPO4. Hydroxyapatite and chitosan were appearing at samples with in-situ and ex-situ methods which indicating by peak matching of XRD (closed lattice parameter, crystal size), overlapping at FTIR spectra and supporting by SEM picture (homogenous and agglomerated particle). However, it still needs further research to examine biocompatibility osteoconductive degree of sample by in-vivo and in-vitro test.
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