Judul: Community Structure of Macrozoobenthos in Seagrass Bed of Bone Batang Island South Sulawesi
Abstrak: Research about Community Structure of Macrozoobenthos in seagrass bed has been done in Bone Batang island. The aim of this research was to investigated the interaction among benthic species and seagrass in seagrass bed as their habitat. A plastic foil (4 x 3 m) were used to collect macrozoobenthos samples. Two types of station were determined. First, seagrass bed as the main station and bare sand (unvegetated area or “blow out”) as control station. Densities of macrozoobenthos from seagrass station and bare sand as control were then compared. Result showed that the highest density of macrozoobenthos collected by plastic foil method, dominated by alpheid shrimp Alpheus macellarius (2.82 individu/m2) in seagrass station and small bivalve Antigona reticulata 1.75 individu/m2) in control station. Bray-Curtis Similarity Index and n-MDS analysis for seagrass community structure among station showed, that all stations tend to have similar seagrass species composition. In contrast with the mention above, macrozoobenthos fauna from all stations showed different species composition. Correlation Analysis using Pearson Product Moment for macrozoobenthos community structure showed that there is no correlation between seagrass density and macrozoobenthos density (r = 0.302). It has been concluded that interaction among benthic species and seagrass as their habitat were influenced by environmental factors rather than direct interaction between seagrass and macrozoobenthos.
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Judul: Keterkaitan padang lamun sebagai pemerangkap dan penghasil bahan organik dengan struktur komunitas makrozoobentos di perairan pulau Barrang Lompo
Abstrak: Seagrass beds is one of ecosystem in coastal waters which is known for its high organic productivity. The productivity derives from litter fall, epiphyte, phytoplankton and suspended particles which trapped among seagrass. Organic material then undergoing a process of decomposition and becomes a source of nutrients. Furthermore, the nutrients will be used by seagrass itself and other aquatic organisms. Macrozoobenthos is a part of the organisms that served as a connector in material cycles and energy flow, from high-level producers to consumers in the waters. The presence of macrozoobenthos in seagrass bed, will enable to support higher fish production in coastal areas. From this starting point, these studies were conducted to analyze the connection between seagrass beds as a producer and organic matter traps and its influenced to macrozoobenthos community structure. The aim of this study is to know the effect of seagrass density toward the total organic material on the bottom substrate, to investigate the effect of seagrass density on the abundance of macrozoobenthos and to determine the influence of total organic material against macrozoobenthos abundance, as well as the rate of deposition of suspended particles (mg/cm2/day). Sampling were taken in the southeast and northeast part of Barrang Lompo Island. Result of the present study, showed that all of 2 stations in Barang Lompo Islands were dominated by 5 species of seagrasses such as Cymodocea serullata, Syringodium isoetifolium, Halophila minor, Halodule uninervis, and Thalassia hemprichii. About 70 genera of macrozoobenthos have been found in all observed stations comprised of 53 families, 7 classes, and 4 phyla. Macrozoobenthos diversity index relatively higher in seagrass beds compared to bare area. However, homogenity index in seagrass beds as well as bare area was high and was relatively homogen. Thalassia hemprichii, Cymodocea serullata and Syringodium isoetifolium are the main species in this area with high densitiy. Results of Principal Component Analysis (PCA) showed that seagrass with higher density were able to trap amount of total organic material in the substrate. Macrozoobenthos density were directly proportional with seagrass density and total organic material. Gastropods are commonly found in seagrass bed with higher density and higher total organic material. In contrast with the results mention above, bivalve are more common in bare area. Coastal waters with higher clay sediment concentration may able to trap higher suspended particles than those within sandy and silty sediment.
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Judul: Optimasi Pemurnian Protein Kapang Endofit CgKTm 5F  sebagai Inhibitor RNA Helikase Virus Hepatitis C
Abstrak: Hepatitis C adalah salah satu penyakit yang menyebabkan peradangan hati. Obat atau vaksin yang efektif untuk pengobatan hepatitis C belum diketahui. Penemuan terapi target molekular NS3 helikase memiliki potensi sebagai salah satu cara untuk penghambatan penyakit hepatitis C. Penghambat NS3 helikase yang memiliki nilai kemurnian yang tinggi masih belum didapatkan sehingga diperlukan penelitian untuk mengoptimasi teknik pemurnian kapang endofit CgKTm 5F sebagai penghambat RNA helikase virus hepatitits C. Pemurnian kapang endofit CgKTm 5F dilakukan dengan menggunakan beberapa macam teknik kromatografi, yaitu gel filtrasi, penukaran ion, hidrofobik dan kromatografi lapis tipis. Kromatografi gel filtrasi dengan sephadex G-50 dan eluen etanol 30% memiliki aktivitas inhibisi tertinggi (89,45%) pada fraksi ke-10. Analisis SDSPAGE menunjukkan bahwa kapang endofit CgKTm 5F adalah pita tunggal sebesar 13,97 kDa dan aktivitas spesifik sebesar 3.62x10-5 U/mg serta persen kemurnian sebesar 99,93%. Kemurnian ini 20x lebih tinggi dibandingkan dengan penelitian sebelumnya sebesar 5,83 %.
Keyword: vaccine therapeutic treatments, NS3 helicase, RNA, SDS-PAGE analysis