Judul: Pemurnian Rekombinan Eritropoietin Manusia (rhEPO) Menggunakan Kromatografi Afinitas Ni-NTA dan Co-Talon
Abstrak: Suitable and efficient techniques for purification of recombinant human erythropoietin (rhEPO) are required to obtain pure rhEPO which can be continued to preclinical trials step. One of these techniques is immobilized metal affinity chromatography (IMAC), which has advantages such as high selectivity, easy to elute, and simple procedure for column regeneration. At least, there are 2 kinds of commercial IMAC resin using nitriloacetic acid (NTA) matrix as chelating agent, namely nickel-based (Ni-NTA) and cobalt-based IMAC resin (Co-NTA), the latter is well known as Talon. This research studied the influence of metal ions Ni2+ and Co2+ in rhEPO purification. The results indicated that Ni- NTA had higher affinity in binding rhEPO protein compared with Co-Talon. Average total protein by Ni-NTA was 226.72 μg/mL and Co-Talon was 60.52 μg/mL, but the protein purity with Co-Talon was better than Ni-NTA. Base on protein yield that can be separated, Ni-NTA is more suitable to obtain high yield of rhEPO protein.
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Judul: Purifikasi dan Pencirian Eritropoietin Rekombinan Hasil Ekspresi pada Sistem Khamir Pichia pastoris
Abstrak: For the clinical purposes needed a purified protein and the carbohydrates contains of recombinant erythropoietin (EPO) should be known. Purification was done by histrap method and then applied to column chromatography gel filtration to make more purified. Carbohydrates groups which is oligosaccharide on purified protein then was characterized by N- and O- glycosidase and the pattern compared with recombinant EPO by CHO cells. The result show has a similar molecular mass which visualized by Western blot method. The purified oligosaccharide were hydrolysed to monosaccharides by incubation in 4 N HCl at 100 ºC for 6 h in evacuated sealed tube. Monosaccharides were separated by high performance liquid chromatography instrument, but unfortunately, the results cannot be shown the kind of monosaccharides.
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Judul: Keberagaman Bambu Marga Schizostachyum Nees di Jawa
Abstrak: Marga Schizostachyum merupakan bambu berdiameter buluh kecil, berdinding tipis, beruas panjang dengan percabangan pada satu buku yang sama besar. Catatan pertama marga Schizostachyum di Jawa sejak tahun 1829 bersamaan dengan terbitnya Schizostachyum blumei Nees. Hingga 2001, tercatat ada 10 jenis bambu Schizostachyum di Jawa yaitu: S. brachycladum (Kruz) Kruz, S. iraten Steudel, S. aequiramosum Widjaja, S. silicatum Widjaja, S. zolingeri Steudel dan Schizostachyum sp. Namun masih terdapat beberapa jenis Schizostachyum yang perlu diidentifikasi dan dicari sebarannya secara alami. Penelitian dilakukan dari September 2017 hingga 2019 dengan metode eksplorasi. Sampel bambu dikoleksi dari Malingping, Lebak, Bogor, Sumedang, Kuningan, Pekalongan, Batang, Purwokerto, Malang, Jember, Probolinggo dan Banyuwangi sebanyak 49 spesimen. Pengamatan morfologi menggunakan 24 karakter vegetatif dan 12 karakter generatif. Analisis data dilakukan dengan SIMQUAL (Similarity for Qualitative Data), SM (Simple Matching) dan SAHN (Sequential, Aglomerative, Hierarchical and Nested), menggunakan metode UPGMA (Unweighted Pair Group Method with Arithmethic Mean) pada software NTSys pc. 2.02i. Dalam penelitian ini, sekitar 13 jenis bambu marga Schizostachyum ditemukan di Pulau Jawa, 6 diantarnya jenis baru, 1 catatan baru dan 1 jenis penemuan kembali. Jenis-jenis bambu Schizostachyum di Pulau Jawa yaitu: Schizostachyum abrorii, S. aequiramosum, S. brachycladum, S. biflorum, S. castaneum, S. iraten, S. jatigedensis, S. hartanai, S. batangensis, S. silicatum, S. sengkol dan S. widjajai. Hasil analisis morfologi menunjukkan bahwa bambu Schizostachyum di Jawa mengelompok menjadi 4 kelompok berdasarkan posisi dan lebar daun pelepah buluh serta bentuk aurikel pelepah buluh. Kelompok pertama terdiri dari Schizostachyum abrorii, S. brachycladum dan S. zollingeri. Kelompok kedua terdiri dari Schizostachyum aequiramosum, S. castaneum dan S. jatigedensis. Kelompok ketiga terdiri dari Schizostachyum batangensis, S. widjajai, S. iraten dan S. sengkol. Kelompok keempat terdiri dari Schisostachyum hartanai, S. biflorum dan S. silicatum.
Keyword: bambu, Jawa, Schizostachyum