Judul: Pengaruh gaya pengasuhan dan hubungan peer group dengan perilaku game online pada siswa SMK
Abstrak: Perilaku kecanduan game online di kalangan remaja berdampak negatif terhadap aspek-aspek kehidupan pada remaja. Penelitian ini bertujuan untuk mengetahui pengaruh gaya pengasuhan dan hubungan peer group terhadap perilaku kecanduan game online di Kota Bogor. Contoh dalam penelitian adalah siswa kelas X dan XI SMK X yang bermain game online diatas 5 jam per hari. Pengambilan contoh menggunakan metode purposive sampling sebanyak 72 orang dari 100 remaja di SMK yang disurvei. Hasil penelitian menunjukkan lebih dari setengah (52,77%) ayah menerapkan gaya pengasuhan otoriter, sedangkan sebanyak kurang dari setengah (40,27%) ibu menerapkan gaya pengasuhan permisif. Hubungan peer group dengan remaja berada pada kategori tinggi. Sebanyak 56.7 persen remaja mengaku lebih suka menghabiskan waktu bersama teman. Remaja dengan perilaku kecanduan game online sebanyak 52.8 persen bermain selama 6-7 jam per hari. Hasil uji regresi menunjukkan uang saku, lama pendidikan ibu dan kebersamaan dengan teman berpengaruh negatif signifikan terhadap perilaku kecanduan game online, sedangkan gaya pengasuhan otoriter ayah dan gaya pengasuhan permisif ibu berpengaruh positif terhadap perilaku kecanduan game online
Keyword: Addiction, Online Game, Parenting Style, Peer group, Delinquency

Judul: Pengaruh Pola Asuh Emosi Orangtua dan Kontrol Diri Remaja terhadap Perilaku Bermain Game Online Remaja Laki-laki dan Perempuan
Abstrak: Penelitian ini bertujuan untuk menganalisis pengaruh pola asuh emosi orangtua dan kontrol diri remaja terhadap perilaku bermain game online pada anak remaja. Penelitian ini melibatkan 60 responden sebagai sampel berusia 16-18 tahun dari SMK Negeri di Kota Bekasi. Pemilihan sekolah dilakukan secara purposive sedangkan pemilihan contoh dilakukan secara non-proportional random sampling. Data dikumpulkan dengan teknik self-report menggunakan kuesioner. Terdapat perbedaan jenis kelamin pada kontrol diri remaja, remaja perempuan memiliki kontrol diri lebih tinggi dibandingkan laki-laki. Pada perilaku bermain game online, remaja laki-laki memiliki perilaku bermain game online yang lebih tinggi dibandingkan perempuan. Hasil uji korelasi menemukan adanya hubungan antara jenis kelamin remaja, pendidikan ibu, dan kontrol diri remaja, dengan perilaku bermain game online pada remaja. Terdapat pengaruh secara langsung oleh kontrol diri remaja dan secara tidak langsung oleh pola asuh emosi melalui kontrol diri terhadap perilaku bermain game online.
Keyword: game online, kontrol diri, pola asuh emosi, remaja, game online, kontrol diri, pola asuh emosi, remaja

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable