Judul: Penyusunan Desain Wisata Minat Khusus Berdasarkan Pola Pergerakan Badak Jawa di Taman Nasional Ujung Kulon
Abstrak: The purpose of this study is to develop a design special interest tourism (SIT) based on the pattern of movement, characteristic of activity sites and behavior patterns of the javan rhino (Rhinoceros sondaicus Desm, 1822) in Ujung Kulon National Park. Data collection was conducted from January until May 2012 in Citadahan, Cibandawoh, Cigenter and Nyawaan of Ujung Kulon Peninsula. Data on movement pattern was collected according to trajectory scheme. Activ sites was compiled from sample plot, while behavioral pattern was captured and identified from video trap data in 2011 and compiled into an ethogram. SIT aspect was acquaired by interview and quitionnare. Minimum outer convex polygon was used for home range calculation, and analysis binomial regression was used to determined characteristic of rhino activity sites while descriptive analysis was used to determined behavioral pattern and SIT design. Javan rhino movement patterns tend to use the same track regularly and create a polygonal pattern. Movement is influence by sex, age and availibity of ideal habitat. Male javan rhino has a larger home range than female, and home range of an independent female rhino is larger than females with calf. Characteristic of rhino activity sites are different between feeding, dung, wallowing and resting places. This research found that the javan rhino active mostly during the night, while wallowing is mainly during the day. Recomendation for SIT design base on javan rhino movement pattern are : a) observation bathing activities of javan rhino on the beach by camping, b) observation bathing activities in the river by canoeing, c) observation wallowing and/or bathing activities by using rangoon
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Judul: Analisis Karakteristik Kubangan Badak Jawa (Rhinoceros sondaicus Desmarest 1822) Di Taman Nasional Ujung Kulon
Abstrak: Javan rhino (Rhinoceros sondaicus Desmarest 1822) is a rare and unique species. Today, its population can only be found in Ujung Kulon National Park. One of insitu conservation efforts to keep the population existence of javan rhino is creating the second habitat. The most important thing to conduct this program is to identify the behaviors of javan rhino. Wallowing is an important behavior of javan rhino. Based on javan rhino’s behavior, the characteristic of its wallow can be identified. The observed characteristics of wallow will determine the standard or criteria of habitat which will be used for selection strategy of further javan rhino’s wallow. The objective of this study was to identify the wallow characteristic of javan rhino both physical and biological factors and to identify the dominant ecological factor in determining wallow selection strategy for javan rhino. The results of this study is hopefully used for the consideration in determining the second habitat of javan rhino and for the basics information of habitat development of javan rhino insitu conservation. The data that were collected consisted of physical characteristic javan rhino’s wallow i.e. the length and the width of wallow, the depth of mud and water of wallow, water pH inside the wallow, the height of wallow site, the temperature and humidity, and the distance from wallow to the coastal, river, and from human access. The biotic characteristic aspects of javan rhino’ wallow that were observed consisted of the number of javan rhino’ feed and the total density of vegetation spread around the wallow according to result of vegetation analysis. The observation result shows that the dominant length of wallow was range from 3-4 meters and the width was range from 2-3 meters. The temperature was about 26-29°C with 67-90% of humidity, water pH of the wallow was normal (pH 7). The height of wallow site was < 100 m above sea level, and mostly found in 1354-2292 meters from the coastal, 702-1012 meters from the river, and 50-370 meters from human access. The total density of vegetation around the wallow was about 8750-53755 ind/ha for the seedling, 800-18607 ind/ha for the sapling, 25- 906 ind/ha for the poles, and 25-174 ind/ha for the tree. The location of 16th and 18th wallows consisted of highest number of feed, which were 35 species of plants. Based on the result of factor analysis, the dominant ecological factors of wallow selection are height of the site, air temperature, and tree density.
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Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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