Judul: Interaksi protein permukaan bakteri gram positif dengan Fe Ign dan FcY menggunakan teknik soft agar(SA) dan serum soft agar(SSA)
Abstrak: Protein permukaan seperti protein A pada Staphylococcus aureus dan protein G pada Streptococcus sp. dapat mengikat imunoglobulin G (IgG). Protein ini merupakan faktor virulensi dari bakteri tersebut (faktor antifagositosis). Penelitian ini dilakukan dengan tujuan untuk menentukan keberadaan protein permukaan pada S. aureus, S. agalactiae, dan S. equi subsp. zooepidemicus dengan metode Soft Agar (SA) dan Serum Soft Agar (SSA). Interaksi dari serum (IgG) dengan protein permukaan pada beberapa bakteri tersebut ditunjukkan dengan perubahan bentuk koloni bakteri dari difus menjadi kompak. Dari 26 isolat S. aureus yang digunakan dalam penelitian, 19 isolat menunjukkan perubahan bentuk koloni setelah penambahan serum kelinci dan 7 diantaranya tidak menunjukkan perubahan bentuk koloni. Tidak ada perubahan yang nyata dari hasil yang ditunjukkan oleh penambahan serum ayam (IgY), semua isolat menunjukkan pertumbuhan koloni difus. Empat isolat Streptococcus grup B (SGB) dan 4 isolat Streptococcus grup C (SGC) yang digunakan dalam penelitian ini tumbuh sebagai koloni difus di Soft Agar dan Serum Soft Agar.
Keyword: Surface proteins, Staphylococcus aureus, Streptococcus sp.

Judul: Modifikasi Enrichment BHI untuk Optimasi Pertumbuhan Staphylococcus aureus Protein A pada Metode Serum Soft Agar.
Abstrak: Metode Serum Soft Agar merupakan salah satu teknik sederhana yang digunakan untuk mengidentifikasi keberadaan protein A pada bakteri S. aureus. Protein A adalah produk spesifik dari bakteri S. aureus dan sekitar 99% dari strain bakteri S. aureus mengandung protein tersebut. Penelitian ini bertujuan untuk membandingkan pertumbuhan koloni bakteri S. aureus pada waktu inkubasi BHI 90 menit (modifikasi) dan 24 jam (kontrol) menggunakan metode serum soft agar. S. aureus yang tumbuh terlihat dari ekspresi protein A berkoloni kompak di serum kelinci tetapi difus di serum ayam. Berdasarkan hasil uji SSA, hanya 13 isolat dari 15 isolat yang digunakan mampu mengekspresikan hasil yang sama dengan kontrol (metode 24 jam) sehingga inkubasi BHI 24 jam masih menjadi metode yang paling baik saat ini untuk pertumbuhan S. aureus.
Keyword: Protein A, SSA, Staphylococcus aureus

Judul: Characteristic of Overshooting Top (OT) Based on  Himawari  8  Satellite  as  a  Convective  Cloud  Feature  that  Generates  Extreme Weather in the Java Island Region
Abstrak: Overshooting Top (OT) is a feature at the top of a convective cloud that is associated with extreme weather. Research on OT in tropical Indonesia is still very limited. The existence of geostationary satellites with wide area coverage and fast frequency observations is useful for observing the fast dynamics of the OT life cycle. Therefore, this study intends to analyze the relationship between OT and extreme weather, updraft and thermodynamic conditions of OT when extreme weather occurs, the best objective method for detecting OT in the day time and night time, and the spatiotemporal distribution of OT in the tropical region of Java Island during the 2018-2021 period. Based on visual analysis using visible channel and Himawari 8 sandwich products, 87% of extreme weather events in Java were accompanied by the appearance of OT. It indicates that the presence of the OT pattern is a convection cloud pattern or feature that is closely related to extreme weather events for the Java Island region. The existence of a time lag between the appearance of OT and extreme weather events on Java Island indicates that the OT of convection cloud can be used as a precursor or nowcasting for the appearance of extreme weather. Most of the convective cloud OT features during extreme weather events on Java Island have altitude that were only able to exceed the altitude of the equilibrium level and few were able to exceed the tropopause level of 9 km – 16 km. The difference in atmospheric conditions during extreme weather events on Java Island, which is in the tropics and subtropics, is a strong and unstable convective energy condition characterized by a larger CAPE, as well as unstable KI, LI, SI and TTI values. It is offset by kinematic factors in the form of a weak vertical wind shear (bulk wind shear 0-6 km) and a higher freezing point height. The difference between extreme weather events in the Java Island region and other tropical regions compared to subtropical regions is the strong updraft speed of convection clouds. The IRW Texture COMB algorithm based on the IRW-Texture Gradient modification and the COMB algorithm shows a significant increase in quality for detecting OT and non-OT pixels in Java. Rainy days with rainfall > 50 mm/day are very common in areas with an altitude of > 500 m. On the other hand, the level of convection activity which is indicated by the presence of OT and lightning is significantly reduced in this area. OT of convective clouds appear more frequently during the DJF season and less frequently during the JJA season. OT often appears during the day time and rarely at night time. Most OTs appear in the central part, away from the south and north coasts of Java Island during the day, while OTs that appear near the south and north coasts of Java Island can appear in the morning or afternoon.
Keyword: convective clouds, extreme weather, Overshooting Tops, Himawari 8 Satellite