Judul: Evaluasi Potensi Hidrolitik Enzim Selulase Dan Glukoamilase Dari Campuran Filtrat Kultur Aspergillus Niger Dan Trichoderma Koningii
Abstrak: Suatu penelitian telah dilakukan untuk mengevaluasi potensi hidrolitik enzim selulase dan glukoamilase dari campuran filtrat kultur Aspegillus niger dan Trichoderma koningii, dan membandingkannya dengan potensi hidrolitik dari masing-masing filtrat kultur. Penelitian ini dilaku- kan selama 5 bulan. Perlakuan diberikan dengan lima taraf yakni filtrat kultur Aspergillus niger (A1), Trichoderma koningii (A2), A1 A2 = 1 : 1 (A3), A1 A2 = 1 : 2 (A4) dan A1 A2 2 1 (A5), dan dicobakan pada empat substrat yakni karboksimetil selulosa (CMC), kertas saring (FP) untuk uji aktivitas enzim selulase; pati kentang dan jagung untuk uji aktivitas enzim glukoamilase. Masing-masing perlakuan mendapat 3 kali ulangan. Filtrat kultur diperoleh dari 2 media fermentasi, fermentasi media cair, yakni fermentasi media padat dan fermentasi media cair. Proses hidrolisis dilakukan dalam kondisi optimum pada pH 4.8 dan suhu 40°C. Kadar glukosa yang dihasilkan sebagai hasil kerja enzim, dijadikan indikator dalam menetapkan aktivitas atau potensi hidrolitik suatu enzim. Dari hasil fermentasi media padat, diperoleh bahwa aktivitas enzim selulase dari filtrat kultur A1P (U= 0.4717) memiliki aktivitas 1.42 kali lebih tinggi dari A2P (U 0.3326) dan A3P (U 0.5809) memiliki = aktivitas 1.23 kali lebih tinggi dari A1P (U = 0.4717) atau 1.74 kali lebih tinggi dari A2P (U 0.3326), ...dst
Keyword: Aspergilus Niger, Trichoderma koningii

Judul: Pemanfaatan Jerami Padi Untuk produksi Enzim Selulase Dari Aspegillus Niger L51/NRRL A-11, 246
Abstrak: Aspergillus niger L51/NRRL A-11,264 ditumbuhkan pada medium jerami padi selama waktu inkubasi tertentu, kemudian dilakukan ekstraksi protein enzim yang dihasilkannya. Pengu- jian dilakukan terhadap aktivitas CMC-ase dan beta-glukosi- dase. Perlakuan delignifikasi dengan larutan NaOH 4 persen, penambahan pepton 1.75 persen, laktosa 0.5 persen dan tween 80 sebesar 0.2 persen ternyata mampu meningkatan aktivitas enzim selulase yang dihasilkan. Unit aktivitas beta-glukosidase tertinggi yang dihasil- kan adalah 4.4780 unit/ml filtrat biakan yang diperoleh dari fermentasi medium padat dan 0.7262 unit/ml filtrat biakan yang diperoleh dari fermentasi medium cair yang diberi perla- kuan delignifikasi, penambahan pepton 1.75 persen dan laktosa 0.5 persen serta tween 80 sebesar 0.2 persen, yang dicapai pada waktu inkubasi sembilan hari. Unit aktivitas CMC-ase tertinggi yang dihasilkan adalah 2.4743 unit/ml filtrat biakan yang diperoleh dari fermentasi medium padat dan 0.4256 unit/ml filtrat biakan yang diper- oleh dari fermentasi medium cair, yang dicapai pada waktu inkubasi enam hari dengan perlakuan yang sama seperti di atas. Aktivitas spesifik beta-glukosidase yang dihasilkan pada penelitian ini adalah 1.5313 unit/mg protein yang diperoleh dari fermentasi medium padat dan 1.3431 unit/mg protein yang diperoleh dari fermentasi medium cair. Sedangkan aktivitas spesifik CMC-ase adalah 1.017 unit/mg protein pada fermentasi medium padat dan 0.6307 unit/mg protein pada fermentasi medium cair. Aktivitas spesifik beta-glukosidase dan CMC-ase yang di- hasilkan pada penelitian ini ternyata lebih baik dari aktivi- tas spesifik enzim NOVO (Celluclast 150 L), yaitu masing- masing 0.0277 unit/mg protein dan 0.3165 unit/mg protein….
Keyword: 

Judul: Characteristic of Overshooting Top (OT) Based on  Himawari  8  Satellite  as  a  Convective  Cloud  Feature  that  Generates  Extreme Weather in the Java Island Region
Abstrak: Overshooting Top (OT) is a feature at the top of a convective cloud that is associated with extreme weather. Research on OT in tropical Indonesia is still very limited. The existence of geostationary satellites with wide area coverage and fast frequency observations is useful for observing the fast dynamics of the OT life cycle. Therefore, this study intends to analyze the relationship between OT and extreme weather, updraft and thermodynamic conditions of OT when extreme weather occurs, the best objective method for detecting OT in the day time and night time, and the spatiotemporal distribution of OT in the tropical region of Java Island during the 2018-2021 period. Based on visual analysis using visible channel and Himawari 8 sandwich products, 87% of extreme weather events in Java were accompanied by the appearance of OT. It indicates that the presence of the OT pattern is a convection cloud pattern or feature that is closely related to extreme weather events for the Java Island region. The existence of a time lag between the appearance of OT and extreme weather events on Java Island indicates that the OT of convection cloud can be used as a precursor or nowcasting for the appearance of extreme weather. Most of the convective cloud OT features during extreme weather events on Java Island have altitude that were only able to exceed the altitude of the equilibrium level and few were able to exceed the tropopause level of 9 km – 16 km. The difference in atmospheric conditions during extreme weather events on Java Island, which is in the tropics and subtropics, is a strong and unstable convective energy condition characterized by a larger CAPE, as well as unstable KI, LI, SI and TTI values. It is offset by kinematic factors in the form of a weak vertical wind shear (bulk wind shear 0-6 km) and a higher freezing point height. The difference between extreme weather events in the Java Island region and other tropical regions compared to subtropical regions is the strong updraft speed of convection clouds. The IRW Texture COMB algorithm based on the IRW-Texture Gradient modification and the COMB algorithm shows a significant increase in quality for detecting OT and non-OT pixels in Java. Rainy days with rainfall > 50 mm/day are very common in areas with an altitude of > 500 m. On the other hand, the level of convection activity which is indicated by the presence of OT and lightning is significantly reduced in this area. OT of convective clouds appear more frequently during the DJF season and less frequently during the JJA season. OT often appears during the day time and rarely at night time. Most OTs appear in the central part, away from the south and north coasts of Java Island during the day, while OTs that appear near the south and north coasts of Java Island can appear in the morning or afternoon.
Keyword: convective clouds, extreme weather, Overshooting Tops, Himawari 8 Satellite