Judul: Lipase Bakteri Asam Laktat Asal Bekasam Pendegradasi Lipid pada Susu Sapi
Abstrak: Susu sapi full cream memiliki kandungan asam lemak jenuh yang tinggi, jika dikonsumsi berlebih dapat menyebabkan hiperlipidemia. Enzim lipase berperan menghidrolisis lipid menjadi asam lemak dan gliserol. Bakteri asam laktat (BAL) merupakan salah satu bakteri penghasil enzim lipase yang dominan ditemukan pada bekasam. Penelitian ini bertujuan memperoleh isolat BAL asal bekasam yang memiliki potensi sebagai pendegradasi lipid pada substrat susu sapi. Sebanyak 18 isolat BAL koleksi Desniar diremajakan kembali pada media MRSA. Sebanyak 6 isolat terpilih dilakukan uji kemampuan penghasil lipase pendegradasi lipid pada media MRSA yang mengandung susu sapi 1% dan rhodamin B 0.002 %. Staphylococcus aureus digunakan sebagai kontrol positif dan Eschericia coli digunakan sebagai kontrol negatif. Dari keenam isolat yang diuji, isolat Lactobacillus plantarum memberikan hasil pendaran terkuat ketika dideteksi dibawah sinar UV. Berdasarkan hasil aktivitas lipase, diperoleh nilai aktivitas unit lipase sebesar 0.555 unit per mL pada jam ke-16. Hasil penelitian ini diharapkan L. plantarum dapat berperan dalam menjaga kesehatan.
Keyword: bekasam, Lactobacillus plantarum, lipase, rhodamine B

Judul: Bakteri Asam Laktat Asal Bekasam Penghasil Lipase Pada Substrat Margarin
Abstrak: Enzim lipase pada mikroorganisme memiliki potensi yang besar di bidang kesehatan, industri dan proses bio-diesel. Mikroorganisme penghasil lipase diantaranya adalah bakteri asam laktat (BAL). Margarin merupakan bahan pangan yang digunakan hampir pada setiap makanan. Penelitian ini bertujuan untuk mendapatkan isolat BAL asal bekasam yang memiliki potensi lipolitik pada substrat margarin. Delapan belas isolat asal bekasam terkoleksi di laboratorium disegarkan kembali pada media MRSA suhu 37ºC selama 48 jam kondisi anaerob. Isolat terpilih diuji potensi penghasil lipolitik pada substrat margarin 1% menggunakan indikator Rhodamine B dan dilihat di bawah UV 365 nm, diidentifikasi nama spesies dengan API KIT 50 CHL. Isolat terpilih diukur kurva pertumbuhan dan aktivitas lipase. Isolat BI(2), BP(3), BP(6), SK(5), dan NS(6) menunjukkan kemampuan mendegradasi lipid pada margarin dengan pendaran warna jingga. Isolat BI(2) memiliki pendaran terkuat. Hasil identifikasi isolat BAL BI(2) adalah Lactococus lactis spp. lactis dengan nilai ID 82.40%. Jumlah sel dan aktivitas unit lipase tertinggi Lactococcus lactis terlihat pada fase log akhir yaitu jam ke-12 sebesar 4.741 CFU/mL dan 29.812 unit/mL.
Keyword: bakteri asam laktat, bekasam, Lactococcus lactis, lipase, margarin

Judul: Characteristic of Overshooting Top (OT) Based on  Himawari  8  Satellite  as  a  Convective  Cloud  Feature  that  Generates  Extreme Weather in the Java Island Region
Abstrak: Overshooting Top (OT) is a feature at the top of a convective cloud that is associated with extreme weather. Research on OT in tropical Indonesia is still very limited. The existence of geostationary satellites with wide area coverage and fast frequency observations is useful for observing the fast dynamics of the OT life cycle. Therefore, this study intends to analyze the relationship between OT and extreme weather, updraft and thermodynamic conditions of OT when extreme weather occurs, the best objective method for detecting OT in the day time and night time, and the spatiotemporal distribution of OT in the tropical region of Java Island during the 2018-2021 period. Based on visual analysis using visible channel and Himawari 8 sandwich products, 87% of extreme weather events in Java were accompanied by the appearance of OT. It indicates that the presence of the OT pattern is a convection cloud pattern or feature that is closely related to extreme weather events for the Java Island region. The existence of a time lag between the appearance of OT and extreme weather events on Java Island indicates that the OT of convection cloud can be used as a precursor or nowcasting for the appearance of extreme weather. Most of the convective cloud OT features during extreme weather events on Java Island have altitude that were only able to exceed the altitude of the equilibrium level and few were able to exceed the tropopause level of 9 km – 16 km. The difference in atmospheric conditions during extreme weather events on Java Island, which is in the tropics and subtropics, is a strong and unstable convective energy condition characterized by a larger CAPE, as well as unstable KI, LI, SI and TTI values. It is offset by kinematic factors in the form of a weak vertical wind shear (bulk wind shear 0-6 km) and a higher freezing point height. The difference between extreme weather events in the Java Island region and other tropical regions compared to subtropical regions is the strong updraft speed of convection clouds. The IRW Texture COMB algorithm based on the IRW-Texture Gradient modification and the COMB algorithm shows a significant increase in quality for detecting OT and non-OT pixels in Java. Rainy days with rainfall > 50 mm/day are very common in areas with an altitude of > 500 m. On the other hand, the level of convection activity which is indicated by the presence of OT and lightning is significantly reduced in this area. OT of convective clouds appear more frequently during the DJF season and less frequently during the JJA season. OT often appears during the day time and rarely at night time. Most OTs appear in the central part, away from the south and north coasts of Java Island during the day, while OTs that appear near the south and north coasts of Java Island can appear in the morning or afternoon.
Keyword: convective clouds, extreme weather, Overshooting Tops, Himawari 8 Satellite