Judul: Mutasi Gen xyncmu dan Pengaruhnya terhadap Stabilitas Enzim Xilanase CMU dari Bacillus halodurans
Abstrak: Applications of xylanase enzyme on paper industry requires an enzyme characteristic through pH and resistant to high temperatures. A directed point mutations in site directed mutagenesis PCR which being the alternative to changing the enzyme’s characteristic and a several of amino acid on parental clon (M3) which effected with enzyme stability. This study aims to alter the stability of xylanase CMU and comparing the characters between CMU xylanase’s mutant and normal. The first step involves a plasmid with parental clones (Clone M3) which transferred to two pairs of mutagenic primers that Q53K and T194I. The amplicons from mutant plasmid pGEMalkxynaq1cmu M3 were transformed into Escherichia coli DH5α and selected with ampicillin media. Results of selection with ampicillin and restriction analysis were confirmed two positive mutant clones are Clone 4 and Clone 7 which brought of xyncmu gene. Gene mutation has succeeded to done as the result was Clone 7 which had enzyme’s characteristic through pH and resistant to high temperatures
Keyword: enzyme stability, CMU xylanase, xyncmu gene, Site directed mutagenesis

Judul: Kloning Gen Xilanase Termozim Dari Streptomyces costaricanus 45I-3
Abstrak: Biopolymer xylan has a great potential to produce useful end product such as xylose. Xylanases is a group of enzyme that has ability to hydrolyse xylan or polymer of xyloses and xylooligosaccharides. Complete hydrolysis of xylans involves the synergistic action of a xylanolytic enzymes, including ß-xylanase, ß-xylosidase, -glucuronidase, -L-arabinofuranosidase, and acetyl xylan esterase. The aims of this research were to isolate xylanase gene from Streptomyces costaricanus 45I-3 and to clone this gene into competence Escherichia coli DH5 . Genomic DNA was obtained from S. costaricanus 45I-3 that have known has ability to produce thermostabile extracellular xylanase. Genomic DNA was partially digested with restriction enzyme Sau3A1. Genomic DNA fragments of size 4-10 kb were inserted at BamHI site in pBluescript II KS (+) vector and transformed into E. coli DH5 . Based on blue-white selection approximately 12000 recombinant colonies were obtained. The presence of xylanase gene was identified by screening employing congored plate clearance assay. One positive clone containing the xylanase gene was confirmed and was designated as pHS11. Preliminary characterization of pHS11 using EcoRI showed that it contained a 6,0 kb DNA insert. Xylanase were produced has xylanase spesific activity 6,393 U/mg, -xylosidase activity 0,208 U/ml, arabinofuranosidase activity 0,079 U/ml and acetyl xylan esterase activity 0,063 U/ml as well.
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Judul: Cycle Hamilton pada Graf Lengkap, Graf Regular, dan Graf 2-Connected 4-Regular Berorder Kurang dari Sepuluh.
Abstrak: Suatu graf disebut graf Hamilton apabila graf tersebut memuat cycle Hamilton, yaitu cycle yang melewati setiap vertex tepat satu kali. Menentukan keberadaan cycle Hamilton pada suatu graf dapat menggunakan syarat cukup yang berupa teorema-teorema yang telah diperkenalkan sejak tahun 1950-an. Beberapa syarat cukup yang dibahas dalam karya ilmiah ini adalah syarat cukup yang berhubungan dengan graf lengkap dan graf regular. ,,,
Keyword: graf Hamilton, graf regular, graf lengkap, graf 2-connected 4-regular.