Judul: The Correlation between Eating Habit, Nutrients Intake and Adequacy, Sleep Quality with Academic Achievement of Nutrition and Economics Students in IPB
Abstrak: Prestasi akademik adalah derajat keberhasilan sebagai bentuk pencapaian belajar setelah mengikuti proses belajar. Penelitian ini bertujuan menganalisis hubungan kebiasaan makan, asupan zat gizi, serta kualitas tidur dengan prestasi akademik pada mahasiswa Gizi dan Ilmu Ekonomi IPB. Desain penelitian menggunakan cross sectional dengan melibatkan 31 mahasiswa Gizi dan 30 mahasiswa Ilmu Ekonomi. Data pengetahuan gizi dikumpulkan menggunakan kuesioner yang diisi sendiri oleh subjek. Data kebiasaan makan dikumpulkan dengan food frequency questionnaires, sedangkan data asupan makanan menggunakan metode recall 2x24 jam. Data kualitas tidur dikumpulkan dengan kuesioner pittsburgh sleep quality index, data konsentrasi belajar menggunakan kuesioner digit symbol sutitution test, sedangkan data prestasi akademik didapatkan dari indeks prestasi kumulatif. Hasil penelitian menunjukkan asupan zat gizi subjek secara keseluruhan kurang dan kebiasaan makannya tidak jauh berbeda. Pengetahuan gizi mahasiswa Gizi lebih baik dibandingkan mahasiswa Ilmu Ekonomi dan terdapat perbedaan nyata (p<0,05). Mayoritas kualitas tidur subjek tergolong buruk. Konsentrasi belajar mahasiswa Gizi (72,5±7,3) lebih baik dibandingkan mahasiswa Ilmu Ekonomi (63,2±11,1) dengan perbedaan nyata (p<0,05). Prestasi akademik sebagian besar subjek tergolong baik. Terdapat korelasi yang signifikan positif antara asupan protein dan lemak dengan konsentrasi belajar serta hubungan konsentrasi belajar dengan prestasi akademik (p<0,05)., Academic achievement is degree of success as a form of learning achievement after following learning process. This research aim to analyze the correlation between eating habit, nutrients intake and sleep quality with academic achievement of nutrition and economics students in IPB. This cross sectional study involving 31 nutrition students and 30 economics students. Nutritional knowledge data was collected using a questionnaire that was filled out by the subjects themselves. Eating habit collected by food frequency questionnaires, while food intake data was collected using 2x24 hour recall. Sleep quality collected by pittsburgh sleep quality index, learning concentration collected by digit symbol substitution test, while academic achievement data was collected by grade point average. The results showed, nutrients intake from most of subject was insufficient and their eating habits were not much different. Nutritional knowledge of nutrition students was better than economics students with significant difference (p<0,05). Majority of subjects’s sleep quality classified as poor. Learning concentration of nutrition students (72,5±7,3) was better than economics students (63,2±11,1) with significant difference (p<0,05). Academic achievement of most subjects is classified as good. There is significant positive correlation between protein and fat intake with learning concentration, and correlation between concentration ability with academic achievement (p<0,05).
Keyword: academic achievement, eating habit, sleep quality

Judul: Relationship between Diet Quality, Sleep Quality, and  Physical Activity with the Nutritional Status of IPB Students.
Abstrak: Permasalahan gizi saat ini pada kelompok dewasa awal adalah permasalahan gizi ganda, yang disebabkan berbagai faktor, diantaranya kualitas diet, kualitas tidur, dan aktivitas fisik. Penelitian ini bertujuan menganalisis hubungan kualitas diet, kualitas tidur, dan aktivitas fisik terhadap status gizi mahasiswa tingkat akhir IPB. Desain penelitian adalah cross sectional study, dilaksanakan Desember 2022 sampai Januari 2023 pada 40 mahasiswa. Pengumpulan data dilakukan dengan wawancara, pengukuran antropometri, dan pengisian kuesioner. Hasil penelitian menunjukkan sebagian besar subjek (68%) memiliki pengetahuan gizi cukup. Hampir seluruh subjek (95%) memiliki kualitas diet rendah berdasarkan Diet Quality Index International (DQI-I). Lebih dari 3/4 subjek (77,5%) memiliki kualitas tidur buruk berdasarkan Pittsburgh Sleep Quallity Indeks (PSQI). Hampir separuh subjek (42,5%) memiliki aktivitas fisik sedang berdasarkan International Physical Activity Questionnaire (IPAQ-SF). Status gizi hampir 2/3 subjek normal (65%). Hasil Uji korelasi Spearman menunjukkan tidak ada hubungan signifikan (p>0.05) antara pengetahuan gizi dengan kualitas diet, kualitas diet dengan status gizi, kualitas tidur dengan status gizi, dan aktivitas fisik dengan status gizi. Terdapat kecenderungan skor pengetahuan gizi baik pada subjek dengan kualitas diet tinggi, kecenderungan skor kualitas diet rendah dan skor kualitas tidur buruk pada subjek dengan status gizi tidak normal. Kata kunci: aktivitas fisik, kualitas diet, kualitas tidur, pengetahuan gizi, status gizi, Current nutritional problem in early adulthood is double burden malnutrition, caused by various factors, including diet quality, sleep quality and physical activity. This study aims to analyze relationship between diet quality, sleep quality and physical activity on the nutritional status IPB final year students. Research design was cross-sectional study, carried out from December 2022 to January 2023 on 40 students. Data collection was carried out by interviews, anthropometric measurements, and filling out questionnaires. Results showed majority subjects (68%) had sufficient nutritional knowledge. Almost all subjects (95%) had low diet quality based on Diet Quality Index International (DQI-I). More than 3/4 subjects (77.5%) had poor sleep quality based on Pittsburgh Sleep Quality Index (PSQI). Almost half of subjects (42.5%) had moderate physical activity based on International Physical Activity Questionnaire (IPAQ-SF). Nutritional status of almost 2/3 subjects was normal (65%). Results of Spearman correlation test showed no significant relationship (p>0.05) between nutritional knowledge and diet quality, diet quality and nutritional status, sleep quality and nutritional status, physical activity and nutritional status. There was tendency for good nutritional knowledge scores in subjects with high diet quality, tendency for low diet quality scores and poor sleep quality scores in subjects with abnormal nutritional status
Keyword: diet quality, nutritional knowledge, nutritonal status, physical activity, sleep quality

Judul: Isolasi Konstruksi dan Rekombinasi Gen Pengkode Reverse Transcriptase asal Simian Retrovirus-2 (SRV-2)
Abstrak: The availability of reverse transcriptase enzym as a valuable component for Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique has been providing by foreign companies. Therefore, it needs more cost and time to provide it. This study is an early step to produce in house reverse transcriptase rekombinant enzym from SRV-2. The purpose of this study was isolated, constructed, and rekombined SRV-2 reverse transcriptase gene to entry clone and destination vector. Isolation was done by PCR amplification technique using three pairs of primers, which are RT I (3282-3683), RTII (3261-3900), and RTIII (3169-4133). Construction and recombination in this study used Gateway method from Invitrogen. SRV-2 reverse transcriptase gene was isolated in this study with A-549 cell inoculated SRV-2 as source of virus. This gene amplify by PCR technique and resulted specific band for RTI 401 bp, RTII 639 bp, and RTIII 964 bp. Analysis of pENTR/SD/TOPO and pDEST17 indicated SRV-2 reverse transcriptase gene code has already inserted to this plasmid. This result indicated SRV-2 reverse transcriptase gene have isolated, constructed, and recombined to entry clone and destination vector.
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