Judul: Flexural behaviour of bamboo truss structure using bolts and fibers joint
Abstrak: Bambu merupakan bahan alternatif sebagai pengganti kayu yang berpotensial untuk dikembangkan sebagai konstruksi. Bambu memiliki sifat fisis dan mekanis yang sama seperti kayu. Namun, bentuk bambu yang silindris berongga dan berbuku menyebabkan masalah saat penyambungan. Tujuan penelitian ini adalah mengkaji perilaku lentur kuda-kuda bambu Andong dan Tali menggunakan sambungan baut dan ijuk pada tiga tipe kuda-kuda. Penggunaan Stucture Analysis Program (SAP) dilakukan untuk melihat dan membandingkan perilaku lentur kuda-kuda melalui program komputer dengan perilaku aktual. Hasil penelitian menunjukkan bahwa kerusakan yang secara umum terjadi pada rangka kuda-kuda sambungan baut dan tali ijuk adalah pecah, retak pada bagian titik buhul, tali kendur bahkan putus akibat adanya pergeseran elemen batang, rusaknya plat sambung dan baut geser. Struktur kuda-kuda bambu Andong sambungan baut mampu menahan beban besar dibandingkan dengan tali ijuk. Tipe kuda-kuda memiliki pengaruh terhadap kemampuan menahan beban, ketiga tipe kuda-kuda yang digunakan memiliki stabilitas yang baik dalam menahan beban. Terjadi perbedaan perilaku lentur berdasarkan nilai defleksi antara pengujian aktual dibandingkan SAP., Bamboo is an alternative material that potentially can be developed as construction. It’s caused bamboo has physical and mechanical properties are similar to wood. However, hollow and nodes were closed to be problems in jointing system. The aims of this research was assess the flexural behaviour of bamboo truss structure using bolts and fibers joint. Use of Stucture Analysis Program (SAP) was conducted to over view and compare the flexural behaviour of the trussess through a computer program and actual behavior testing. The results showed that generally the damage occurs using bolts joint were checks, shake, splits on connected plate, while the damage on fibers joints were checks, shake, slack and break of fibers. The structure of truss bamboo Andong using bolts join have the capable to hold great loads cells than fibers joint. Type of trusses have an influence on load bearing capability, all type of trusses has high stability to hold loads better. The difference of flexural behaviour has occurred between SAP and actual testing based on deflection.
Keyword: Bambu, perilaku lentur, sambungan, SAP

Judul: The effect of connection type, the position of testing, and the distance of connection to the strength of tali bamboo (gigantochloa apus kurzt)
Abstrak: Gigantochloa apus Kurzt yang dikenal dengan bambu tali atau bambu apus adalah salah satu jenis bambu yang mudah ditemukan dan banyak dimanfaatkan oleh masyarakat, khususnya masyarakat pedesaan. Pemanfaatan bambu tali sebagai bahan konstruksi belum banyak digunakan, karena masih kurangnya dukungan data penelitian mengenai karakteristik bambu tali serta modifikasi produk bambu tali dalam bidang konstruksi. Tujuan dari penelitian ini adalah menganalisis karakteristik balok bambu tali menggunakan alat sambung kombinasi pasak dan tali, serta alat sambung baut, pada posisi pengujian horizontal dan vertikal, dengan tiga variasi jarak sambungan (50, 100, dan 150) cm. Prosedur pengujian sifat lentur balok bambu menggunakan standar pengujian ISO 22157-1:2004 tentang pengujian buluh bambu secara full scale. Nilai lentur diperoleh menggunakan persamaan ASTM D-198 dan persamaan ISO 22157-1:2004. Hasil penelitian menunjukkan rerata nilai KA pada bambu tali sebesar 25.70% sampai 40.81% dan rerata nilai kerapatan contoh uji produk sebesar 0.67 g/cm3. Hasil uji mekanis menunjukkan rerata nilai MOE pada balok bambu berada pada selang 93425.16 – 165000.14 kg/cm2 (ASTM D-198) serta 166761.32 - 714347.73 kg/cm2 (ISO 22157-1:2004) dan rerata nilai MOR balok bambu berada pada selang nilai 236.48 – 571.29 kg/cm2 (ASTM D-198) serta 260.69 - 711.09 kg/cm2 (ISO 22157-1:2004). Hasil penelitian menunjukkan bahwa balok bambu perlakuan sambungan baut pada posisi pengujian horizontal merupakan balok bambu dengan perlakuan terbaik. Berdasarkan hasil penelitian ini balok bambu dapat dimanfaatkan sebagai bahan konstruksi ringan., Gigantochloa apus Kurzt known as Tali bamboo or Apus bamboo is one type of bamboo that are easy to find by the people, especially the rural communities. Utilization of bamboo as a construction material has not been widely used because the lack of data about the characteristics of bamboo and it’s modifications of tali bamboo products in construction sector. The purpose of this study is to analyze the characteristics of tali bamboo using combination of dowell and rope connection, and bolt, at horizontal and vertical position, with three distance of connection variation (50, 100, and 150 cm). The bending test method was used by ISO 22157- 1:2004 for full scale test of bamboo’s culm. Bending value was obtained from the equation of ASTM D-198 and ISO 22157.1:2004. The result showed that the average of Moisture Content (MC) of Tali bamboo in range 25.99% to 36.62% and the density was 0.67 g/cm3. The results of mechanical test showed that the mean value of tali bamboo on MOE were 93425.16 – 165000.14 kg/cm2 (ASTMD 198) and 166761.32 - 714347.73 kg/cm2 (ISO 22157-1:2004) and the MOR were 236.48 – 571.29 kg/cm2 (ASTMD 198) and 260.69 - 711.09 kg/cm2 (ISO 22157-1:2004). Based on the results of this research, this bamboo beam can be used as a respectively construction material.
Keyword: Gigantochloa apus Kurzt, sambungan baut, sambungan pasak, sambungan tali, sifat fisis, sifat mekanis

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
Keyword: