Judul: Pengaruh Dukungan Sosial dan Strategi Koping terhadap Ketahanan Keluarga pada Pernikahan Jarak Jauh
Abstrak: Pengaruh Dukungan Sosial dan Strategi Koping terhadap Ketahanan Keluarga pada Pernikahan Jarak Jauh. Dibimbing oleh TIN HERAWATI dan DEFINA. Pasangan suami istri umumnya tinggal dalam satu rumah. Namun, ada kalanya keluarga tidak tinggal bersama yang disebabkan pasangan tersebut merantau untuk bekerja. Kondisi ini menyebabkan suami istri mengalami kesulitan dalam menjaga ketahanan keluarga. Tujuan penelitian adalah menganalisis pengaruh dukungan sosial dan strategi koping terhadap ketahanan keluarga pada pernikahan jarak jauh. Responden dalam penelitian ini adalah 60 istri yang menjalani pernikahan jarak jauh di eks-Karesidenan Pekalongan. Metode penelitian ini adalah non-probability sampling dengan teknik voluntary sampling. Data dikumpulkan melalui google form dengan kuesioner online. Hasil penelitian menunjukkan bahwa dukungan sosial berada pada kategori rendah, sedangkan strategi koping dan ketahanan keluarga pada kategori sedang. Strategi koping yang sering dilakukan kelurga adalah strategi koping yang berfokus pada emosi. Terdapat hubungan positif signifikan antara strategi koping dengan ketahanan keluarga. Faktor yang berpengaruh positif signifikan terhadap ketahanan keluarga yaitu lama pendidikan suami dan strategi koping., The Influence of Social Support and Coping Strategies on Family Strenght in Long Distance Marriages. Supervised by TIN HERAWATI and DEFINA. Married couples generally live in one house. However, there are times when the family does not live together because the couple migrates to work. This condition causes husband and wife to experience difficulty in maintaining family strenght. The research objective was to analyze the influence of social support and coping strategies on family strenght in long-distance marriages. Respondents in this study were 60 wives who had long-distance marriages in the ex-Residency of Pekalongan. This research method is non-probability sampling with voluntary sampling technique. Data was collected through google form with online questionnaire. The results showed that social support was in the low category, while coping strategies and family strenght were in the medium category. The coping strategy that is often used by the family is a coping strategy that focuses on emotions. There is a significant positive relationship between coping strategies and family strenght. Factors that have a significant positive effect on family strenght are the husband's length of education and coping strategies.
Keyword: dukungan sosial, ketahanan keluarga, pernikahan jarak jauh, strategi koping, coping strategies, family strenght, long distance marriage, social support

Judul: The Influence of Stress Symptoms and Coping Strategy on The Subjective Well-Being of Long Distance Marriage Families
Abstrak: Istri yang menjalani long distance marriage atau hidup jauh dari suami merupakan tantangan yang cukup berat dan berakibat pada kelelahan psikologis yang dapat memicu stres dalam hubungan pernikahan. Penelitian ini bertujuan untuk menganalisis pengaruh gejala stres dan strategi koping terhadap kesejahteraan subjektif keluarga long distance marriage. Pendekatan yang digunakan dalam penelitian ini adalah kuantitatif dengan desain cross-sectional. Penelitian ini melibatkan keluarga yang menjalani long distance marriage dengan kriteria lama berpisah > 6 bulan, mempunyai anak remaja (11-21 tahun) dan berdomisili di Kota Bandung. Responden dalam penelitian ini adalah istri. Penarikan contoh dalam penelitian ini menggunakan non-probability sampling dengan teknik snowball sampling. Hasil penelitian menunjukkan gejala stres tergolong rendah,strategi koping tergolong sedang, dan kesejahteraan subjektif tergolong tinggi. Hasil uji korelasi menunjukan usia ayah dan intensitas pertemuan memiliki hubungan positif signifikan dengan strategi koping, intensitas pertemuan memiliki hubungan positif signifikan dengan gejala stres, usia anak memiliki hubungan positif signifikan dengan kesejahteraan subjektif. Gejala stres berhubungan negatif signfikan dengan kesejahteraan subjektif. Problem focused coping dan strategi koping memiliki hubungan positif signifikan dengan kesejahteraan subjektif. Hasil uji regresi menunjukkan gejala stres berpengaruh negatif signfikan sedangkan strategi koping berpengaruh positif signifikan terhadap kesejahteraan subjektif., The wife does it long distance marriage or living away from husband is quite a challenge and results in psychological fatigue which can trigger stress in the marital relationship. This study aims to analyze the influence of stress symptoms and coping strategies on the family's subjective well-being long distance marriage. The approach used in this research is quantitative by design cross-sectional. This research involved families who underwent long distance marriage with the criteria of length of separation > 6 months, having teenagers (11-21 years) and domiciled in Bandung City. The respondent in this study was the wife. Sampling in this research uses non-probability sampling with technique snowball sampling. The research results showed that stress symptoms were relatively mild, coping strategies were moderate, and subjective well-being was relatively high. The correlation test results show that father's age and meeting intensity have a significant positive relationship with coping strategies, meeting intensity has a significant positive relationship with stress symptoms, child age has a significant positive relationship with subjective well-being. Stress symptoms were significantly negatively related to subjective well-being. Problem focused coping and coping strategies have a significant positive relationship with subjective well-being. The results of the regression test show that stress symptoms have a significant negative effect while coping strategies have a significant positive effect on subjective well-being.
Keyword: Gejala stres, Strategi koping, Kesejahteraan Subjektif, Pernikahan jarak jauh, Remaja

Judul: Characterization and Expression of Tomato infectious chlorosis virus Coat Protein Gene in Escherichia coli
Abstrak: Tomato infectious chlorosis virus (TICV) is a member of Crinivirus genus. TICV infects tomato crop in Garut and Cipanas, West Java Indonesia. Tomato initially showed bright interveinal yellowing symptoms. The coat protein gene (CP-TICV) was amplified by RT-PCR from total RNA extracted from infected tomato leaves and the amplified fragment was cloned and completely sequenced. The fragment was subsequently subcloned into the pET-21b expression vector. The recombinant plasmid was transformed to Escherichia coli strain BL21(DE3)pLysS to express the coat protein. The coat protein fused to a 6xhistag, was purified by affinity chromatography using a NiNTA spin column. The identity of the purified protein was confirmed by SDS-PAGE. In this experiment, 792 bp of CP-TICV gene of TICV virus has been successfully cloned, sequenced, and expressed in E. coli. Based on nucleotide sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain strain and 99% identity to North America, France, and California strains and based on amino acid sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain, North America, France strains and 99.2% identity to California strain. CP-TICV showed over expressed in E. coli when it is induced with 1 mM IPTG and incubated at 37oC. Purified CP-TICV-Histag recombinants protein sized 29 kDa based on SDSPAGE analysis.
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