Judul: Pengembangan Spektrofotometer Sinar Tampak (KuantiVis) Berbasis Komponen Elektronik Umum dan Uji Kinerjanya
Abstrak: Spectrophotometer can be developed by replacing the main components with electronic components that are easy to find. This study aims to develop visible spectrophotometer (KuantiVis) device using light emitting diode lamp as light source, optical layer of digital versatile disks as grating, and web camera with charge-couple device sensor as detector. KuantiVis was tested for its performance based on linearity, limit of detection, limit of quantitation, and accuracy parameters using a standard of chromium. The optimum result showed that KuantiVis was made with dimensions of 33 × 16 × 5 cm and distance between web camera and source of light was 25 cm. The result showed that linearity of data based on R2 value was 0.9973, limit of detection was 0.1231 ppm, limit of quantitation was 0.4102 ppm, and recovery was 103.19%. The performance was not much different from the performance of Spectronic 20D+ and Genesys spectrophotometer. The KuantiVis was applied for determining borax concentration in wet noodles sample. Concentration of borax in sample was 28.60 μg/gram samples. The result of F test and t test showed that the data was not significantly different with that of Spectronic 20D+ and Genesys spectrophotometer.
Keyword: Bogor Agricultural University (IPB), visible spectrophotometer, light emitting diode, digital versatile disks, charge-couple device, borax

Judul: Pengembangan dan Pengukuran Kinerja Analitik Spektrofotometer Sinar Tampak ‘KuantiVis’ Berbasis Detektor Charge-couple Device
Abstrak: The advancement of technology provides alternative ways to develop spectrophotometer using simple components such as web camera with chargecouple device detector. The objective of the research is to develop visible spectrophotometer ‘KuantiVis’ with simple electronic components and web-based software Spectral Workbench as data acquisition system. The performance of KuantiVis was determined by validation of methyl red analysis and rhodamine B measurement in food sample (pacar cina). The whole result was compared with analysis using Spectronic 20D+ and Genesys 10UV. The analytical performance of KuantiVis for methyl red showed linearity with R2 was 0.9629, limit of detection was 0.6953 ppm, limit of quantitation was 2.1070 ppm, recovery was 110.00%, and relative standard deviation was 7.59%. The overall results met the Association of Analytical Communities standard but have not equal to the performance of Spectronic 20D+ and Genesys 10UV. Rhodamine B in pacar cina was 0.0022 (%w/w) based on measurement using KuantiVis. This result was not significantly different at the confidence level 95% using t test and F test as compared to the result using Spectronic 20D+ and Genesys 10UV.
Keyword: visible spectrophotometer, rhodamine B, methyl red, charge-couple device, Bogor Agricultural University (IPB)

Judul: Posisi Filogenetik Kura-Kura Carettochelys Insculpta  (Reptillia: Testudinata)
Abstrak: Carettochelys insculpta is an endemic freshwater turtle of southern Papua and northern Australia. Phylogenetic position of this turtle is remain unresolved until now. Most of the research conducted show close relationships of C. insculpta and members of Trionychidae family. However, some of the research show distance between C. insculpta and members of Trionychidae family. This research is conducted to analyze phylogenetic relationship between C. insculpta and other members of Testudinata using 12S rRNA, 16S rRNA, and both gene as markers. DNA samples were obtained from three different organisms of C. insculpta. Two species used as outgroups were Alligator mississippiensis and Iguana iguana. Phylogenetic analysis was carried out using Neighbor-Joining method with Felsenstein 1981 (F81) modeltest. A polytomy tree of Testudinata was produced by using 12S rRNA gene. However, analysis using 16S rRNA and both genes as markers confirmed that C. insculpta was located between the Chelonidae and the Trionychidae family.
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