Judul: Efektivitas fiksasi N2 dan oksidasi metan kultur campuran bakteri metanotrof asal sawah
Abstrak: CH4 emissions in a rice field and wetlands is mostly produced by methanogenic bacteria and 80% of the CH4 is oxidized by methanotrophs. Besides of their ability to oxidize methane, methanotrophs also have ability to fix nitrogen. Four selected isolates of methanotrophs which had high nitrogen fixation activity i.e.(BGM 1, BGM 3, BGM 5, and BGM 9 isolates were combined with SKM 14 isolate which had high methane oxidation activity to obtain ideal formulations of the methanotrophs that have high activity of nitrogen fixation and methane oxidation activities. Nitrogen fixation activity was determined by measuring accumulated ammonium in medium and acetylene reduction assay (ARA). The highest concentraion of ammonium on single culture of methanotrophic bacterium was performed BGM 1 isolate (1,86 μM ammonium), and mixed culture of BGM3 and BGM9 isolates had the highest ammonium accumulation (4,905 μM ammonium). The highest nitrogenase activity was performed by mixed culture of BGM1 and SKM14 isolates, it was 11,27 μmol/ml cultures/hour. Whereas the higest methane oxidation was performed by mixed culture of BGM5 and BGM9 isolates, it was 18,20 ppm /ml cultures /day.
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Judul: Aktivitas fiksasi nitrogen dan oksidasi metan kultur campuran bakteri metanotrof pada lumpur sawah
Abstrak: Metanotroph bacteria are methane oxidizing bacteria. Besides, type II and type X of methanotrophic bacteria were also able to fix nitrogen using nitrogenase enzyme. This reasearch was conducted to test the activity of nitrogen fixation and methane oxidation of five isolates of methanotropic bacteria i.e. BGM 1, BGM 3, BGM 5, BGM 9, and SKM 14 which combined with one another. Seven combinations (SKM 14/BGM 1; SKM 14/BGM 3; SKM 14/BGM 5; SKM 14/BGM 9; BGM 9/BGM 1; BGM 9/BGM 3; BGM 9/BGM 5) were cultured and inoculated into NMS medium mixed with slurry of mud, then the activity of methane oxidation, nitrogen fixation and nitrogenase enzyme activity was tested. The highest accumulation of ammonium in the mud sterile treatment (35.489 μM) was higher than that of in non-sterile mud treatment (26.617 μM). Formulation 6 (BGM 9/BGM 3) in sterile mud treatment and formulation 4 (SKM 14/BGM 9) in non-sterile mud treatment has the highest nitrogenase enzyme activity, it was 5,16 mmol/h/ml culture and 75,85 mmol/h/ml culture respectively. Formulation 1 (SKM 14/BGM 1) and formulation 2 (SKM 14/BGM3) had the higest activity of methane oxidation in sterile and non sterile mud treatment i.e. 17428 and 16759 ppm /ml culture /day respectively.
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Judul: Warna bulu kucing ras persia: variasi pola warna dan pewarisannya
Abstrak: Kucing Persia mempakan kucing rasyang paling urnum dijumpai di Indonesia. Kucing Persia memiliki bulu yang panjang dengan variasi wama bulu yang sangat beragam. Perkawinan yang lebih terarah sangat perlu dilakukan untuk meningkatkan kualitas genetik warna dan pola wama bulu kucing. Penelitian ini bertujuan untuk rnempelajari variasi warna dan pola wama bulu kucing Persia yang terdapat di Indonesia, yang dilakukan di kantor pusat Cat Fancy Indonesia mulai bulan Oktober 2000 sampai Januari 2001. Data yang digunakan rnempakan data pedigree kucing Persia yang tercatat di Cat Fancy Indonesia dari tahun 1997 sampai Oktober 2001 yang terdiri dari 187 jantan dan 258 betina, selain itu juga dilakukan pengamatan langsung kepada tiga penyayang dan breeder kucing yang ada di Bandung.
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