Judul: Isolasi Promotor Gen LFY pada Tanaman Theobroma cacao L. dengan Teknik Genome WalkingTM
Abstrak: Cacao is one of the main agricultural commodities in Indonesia. Unfortunately, there are many obstacles faced by Indonesian cacao farmers, which results in the low cacao productivity. A major obstacle is the cherelle wilt disease. This disease is affected by the central flowering gene (gene LFY), whose expression level is regulated by its promoter. The objective of this research is to isolate the promoter of gene LFY, to then be cloned into its cloning and expression vectors. The promoter isolation was done from a young cacao leaf. The DNA in the leaf was extracted first, and then the promoter was isolated using Genome WalkingTM technique. Subsequently, the sequencing method and Softberry software were utilized to determine the promoter’s nucleotides. The result, which was then confirmed by TATA box, proved the existence of gene LFY promoter fragment. A fragment of 1650 bp size was then cloned into cloning and expression vectors: pGEM-T Easy and pCambia 1303. Next, the plasmid of the recombinant DNA was transformed into Agrobacterium tumefaciens using the AGLO strain. The conclusion of this research is that the gene LFY promoter of Theobroma cacao L. can be isolated using the Genome WalkingTM method, enabling more information on the occurence of cherelle wilt on cacao plants.
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Judul: Isolasi dan Karakterisasi Fragmen Gen LEAFY dari Bantalan Bunga Kakao
Abstrak: Cocoa plant productivity in Indonesia is still low. Information and knowledge at the molecular level about the process of development of regenerative organs of cocoa can be an effective solution to problems associated with the regenerative low productivity of cocoa plants.LEAFY gen is key gene in the shoot meristem transition to flower one. The purposes of the research were to isolate LEAFY gene from cacao’s flower cushion and analyse its expresion in several cacao’s tissues. Isolation of LEAFY gene using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) technique showed that three spesific primer pairs designed based conserved area can amplify the target gene. Futhermore this fragment were inserted into pGEM-T Easy vector then transformed into competent cell E. Coli XL-1 Blue. The result of bioinformatic analysis showed that the fragment of target gene has a high homology to LEAFY gene of other plants in gene bank’s database. Characterization of target gene was initiated with RNA isolation phase from several cacao’s tissues then those were amplified with RT-PCR technique. Amplification at 62 ºC of annealing temperature for 33 cycles was successful to show the existence of LEAFY gene in several cacao’s tissues isolated.
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Judul: Analisis Kekuatan Pondasi Tiang Pancang terhadap Beban Gempa pada Gedung RSUD Kota Depok Berdasarkan SNI 1726:2012
Abstrak: Pondasi adalah bagian terendah dari bangunan yang meneruskan beban bangunan ke tanah atau batuan yang ada di bawahnya. RSUD Kota Depok yang dibangun pada tahun 2008 perlu dievaluasi kekuatannya terhadap beban gempa berdasarkan SNI 1726:2012. Berdasarkan penelitian, nilai kapasitas dukung ultimit tiang sebesar 388.8 ton dan daya dukung ijin sebesar 129.6 ton. Daya dukung aksial kuat menahan beban aksial kecuali pada pondasi jenis PC18. Nilai daya dukung aksial pemodelan pada PC18 sebesar 19 991.99 kN sedangkan hasil perhitungan sebesar 15 424.33 kN. Nilai daya dukung lateral (Hu) tiang pancang sebesar 1132.13 kN dan nilai daya dukung lateral pemodelan terbesar berada pada jenis PC7 pada arah gaya Y (Hy) dengan nilai 802.70 kN. Penurunan tunggal pondasi pada gedung RSUD Kota Depok sebesar 1.17 cm. Penurunan kelompok terbesar terjadi pada PC18 sebesar 5.17 cm. Penurunan izin pada PC18 sebesar 58.5 cm. Nilai daya dukung dan penurunan pondasi hasil perhitungan lebih besar dibanding nilai yang didapat dari pemodelan melalui program ETABS, maka dapat disimpulkan daya dukung dan penurunan pondasi RSUD Kota Depok aman.
Keyword: daya dukung, gempa, penurunan pondasi, tiang pancang