Judul: Identifikasi Keragaman Gen Growth Hormone Receptor (GHR|Eco72I) pada Ayam Lokal Indonesia dan Persilangannya.
Abstrak: Gen growth hormone receptor (GHR) merupakan salah satu gen pengontrol sifat pertumbuhan. GHR sebagai reseptor growth hormone (GH) yang terlibat dalam regulator laju pertumbuhan dan deposisi lemak. Mutasi pada intron 5 mempengaruhi laju pertumbuhan pada ayam. Penelitian ini bertujuan mengidentifikasi keragaman gen GHR pada ayam lokal Indonesia dan persilangannya. Sampel yang digunakan dalam penelitian sebanyak 178 ekor ayam yang terdiri atas ayam kampung Ciawi, kampung Sukabumi, ras pedaging, ras petelur, merawang, dan kampung x ras petelur. Amplifikasi gen GHR|Eco72I intron 5 dilakukan dengan metode PCR-RFLP. Hasil produk amplifikasi adalah 326 bp. Genotipe yang ditemukan pada gen GHR|Eco72I adalah AA, GG, dan AG dengan 2 macam alel yaitu alel A dan alel G. Hasil analisis menunjukkan bahwa frekuensi genotipe AA lebih tinggi dibandingkan dengan genotipe lainnya. Frekuensi alel tertinggi adalah alel A sebesar 0.99 dan terendah adalah alel G sebesar 0.01. Populasi ayam kampung Sukabumi dan persilangan kampung x ras petelur berada pada keadaan keseimbangan Hardy-Weinberg. Nilai heterozigositas pengamatan (Ho) pada ayam ras pedaging lebih rendah dari nilai heterozigositas harapan (He), sedangkan nilai heterozigositas pengamatan (Ho) pada ayam kampung Sukabumi dan kampung x petelur lebih tinggi dari nilai heterozigositas harapannya (He). Kesimpulan penelitian ini adalah gen GHR|Eco72I pada ayam kampung Sukabumi, ras pedaging, dan kampung x ras petelur bersifat polimorfik.
Keyword: ayam lokal, gen GHR, keragaman, PCR RFLP

Judul: Keragaman Gen Hormon Pertumbuhan pada Ayam Lokal Indonesia dan Persilangannya
Abstrak: Indonesia local chickens have genetic potency with high ecomonic value such as meat and egg production, adapted in tropic and strong immunity. The chicken growth hormone (cGH) gene has a crucial role in controlling growth and metabolism, leading to potential correlations between cGH gene polymorphism and economic traits. This study aimed to identify the cGH gene polymorphism and find out the variation of gene at several local chickens, the crossbred, commercial meat type and layer. Polymorphism in intron 4 of cGH gene was studied in 229 chicken blood samples consisted of kampung, pelung, sentul, commercial meat type, layer, merawang, commercial meat type x kampung, kampung x commercial meat type, sentul x kampung and pelung x sentul chickens. A specific primer set was used to amplify a fragment of growth hormone locus using PCR. PCR products were digested with MspI restriction endonucleases. The amplified fragment digested with MspI enzyme revealed 2 alleles A and B with the frequency of 0.88-0.96 and 0.04-0.12, except in layer chickens only had 1 allele A with the frequency of 1.00. The results showed that cGH gene in MspI locus at kampung, pelung, sentul, commercial meat type, merawang, commercial meat type x kampung, kampung x commercial meat type, sentul x kampung and pelung x sentul chickens were polymorphics, except the layer chickens was monomorphics. The population of Indonesia local chicken and the crossbred that was studied in this research had low heterozygosity value but it was still in the Hardy-Weinberg equilibrium.
Keyword: polymorphism, PCR-RFLP, local chicken, MspI, cGH

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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