Judul: Deteksi Jenis Makanan Ikan Kerapu (Epinephelus sp.) dengan DNA Metabarcoding berdasarkan Database Genetik dari Perairan Terumbu Karang, Raja Ampat.
Abstrak: Ikan kerapu merupakan salah satu komoditas ikan karang yang bernilai ekonomi tinggi dan memiliki peran penting ekologis sebagai top predator. Ikan kerapu memiliki kerentanan terhadap kegiatan eksploitasi yang berlebihan, sehingga perlu dilakukan pengelolaan yang tepat terhadap sumberdaya ikan kerapu yang ada di Indonesia. Penelitian ini bertujuan untuk validasi jenis ikan kerapu dan menentukan jenis makanan Epinephelus bleekeri dengan dua database genetik, yaitu MIDORI dan NCBI yang diharapkan dapat digunakan sebagai dasar dalam menentukan pengelolaan. Metode yang digunakan adalah DNA metabarcoding berdasarkan marka gen COI. Jenis makanan yang didapat dengan analisis berdasarkan database NCBI terdiri atas Chordata, Arthropoda, Moluska, dan Cnidaria. Jenis makanan yang didapat dengan analisis berdasarkan database MIDORI terdiri atas Chordata, Arthropoda, dan sebagian masih teridentifikasi sebagai eukaryota. Jenis makanan didominasi oleh Epinephelus bleekeri dari filum Chordata, berdasarkan kedua database genetik yang digunakan. Hasil penelitian ini memberikan informasi dasar, bahwa informasi genetik spesies yang ada di perairan Indonesia masih sangat minim pada database MIDORI.
Keyword: analisis DNA, DNA Shield, HCO-2198, LCO-1490

Judul: Aplikasi DNA Barcoding dan Metabarcoding pada Ikan Kerapu Lumpur (Epinephelus coioides) dan Jenis Makanannya dari Perairan Raja Ampat
Abstrak: Makanan merupakan sumber nutrisi bagi makhluk hidup tidak terkecuali untuk ikan dalam mempertahankan keberlangsungan hidup. Oleh karena itu perlu adanya pengkajian makanan melalui kebiasaan makanan ikan. Penelitian ini bertujuan mengidentifikasi spesies ikan kerapu lumpur Epinephelus coioides melalui analisis DNA Barcoding serta keragaman jenis makanannya menggunakan analisis Metabarcoding dengan marka molekular gen COI. Sampel jaringan dianalisis dengan metode Sanger. Hasil identifikasi spesies melalui DNA Barcoding menunjukkan terdapat basa nukleotida sebanyak 643 pb dengan tingkat kemiripan sebesar 100%. Satu sampel isi perut ikan dianalisis dengan teknologi Next Generation Sequencing. Hasil penelitian menggunakan software MEGA 5.02 dari sampel isi perut didominasi oleh kelompok ikan sebesar 91,80% dan kelompok invertebrata sebesar 8,18%. Hasil serupa menggunakan software QIIME2 dengan referensi database MIDORI LONGEST, makanan didominasi oleh kelompok ikan E. coioides dengan frekuensi relatif berkisar 96% dan ivertebrata sebesar 2%.
Keyword: identifikasi molekular, ikan komersil, isi perut, kanibalisme, nokturnal

Judul: Enhancement of photon absorbtion on BaxSr1-xTiO3 thin films semiconductor using photonic crystals.
Abstrak: In this research, has been conducted BaxSr1-xTiO3 (BST) thin films manufacturing for mole fraction x = 0.25, 0.35, 0.45, and 0.55; and concentration of 1 molar using chemical solution depositon (CSD) method. The materials, which are used in this research, are barium acetate [Ba(CH3COO)2, 99%], strontium acetate [Sr(CH3COO)2, 99%], titanium isopropoxide [Ti(C12O4H28), 97.999%], gallium trioxide [Ga2O3], 2-methoxyethanol [H3COOCH2CH2OH, 99%] and 1 x 1 cm of transparent conductive oxide (TCO) glass as substrate. Two BST thin films are made that for BST thin films which are doped by 10% galium (BGST) as p-type layer and pure BST solution as n-type. BST solution is made by reacting barium acetate [Ba(CH3COO)2, 99%], strontium acetate [Sr(CH3COO)2, 99%] and titanium isopropoxide [Ti(C12O4H28)] into 2-methoxyethanol solvent [H3COOCH2CH2OH, 99%] in accordance with mass gram for each x value. While, BGST solution is made in the same way as BST solution, plus adding gallium at 10% of the mass of BST. Once all materials are reacted, then vibrated using ultrasonic for 90 minutes, and followed by coating BGST / BST on the TCO substrate. Once the layer is formed, then annealed for 15 hours at temperature of 500 °C with increasing rate of 1.67 oC/minute. Characterizations of BST thin films with or without photonic crystals include the absorbance and transmittance in the visible light spectrum, as well as the electrical photoconductivity characterizations. From BST thin films without photonic crystals indicate that our thin films have light absorption area on almost the entire range of the visible light spectrum even to the infrared range with absorption value over than 67.6%, in addition, it can be seen that the maximum absorbance of BST thin films for all mole fractions lie in blue to green light spectrum region, 457 nm ≤ λ ≤ 570 nm and the minimum in yellow to red light spectrum region, 570 nm ≤ λ ≤ 678 nm. The largest absorbance is found at x = 0.25 with average absorbance value of 92.04% and maximum and minimum absorbance peaks at wavelength of 500.89 and 657.72 nm, respectively. At x = 0.35, average absorbance value decreased to 83.55% with maximum and minimum peaks at 505.73 and 613.68 nm, respectively. Then increased to 91.16% at x = 0.45 with maximum and minimum peaks at 506.04 and 618.56 nm, respectively. At x = 0.55, average absorbance value decreased to 80.12% with maximum and minimum peaks at 507.85 and 608.79 nm, respectively. Moreover, it can be seen that the addition of barium mole fraction leads to wider maximum absorbance or minimum transmittance peaks and narrower minimum absorbance or maximum transmittance peaks in the same range of the green and blue & red light spectrums, respectively. BST thin films with photonic crystals show increasing in absorption for all x values in the range of 3.04 into 13.33%. At x = 0.25, average absorbance percentage increased 3.96% from 92.4 into 95.68%, for x = 0.35 average percentage increased 7.07% into 89.45 from 83.55%. Furthermore, average absorbance of BST thin films at x = 0.45 increased 3.04% from 91.16 into 93.93% and x = 0.55 has
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