Judul: The Relationship Between Nutritional Knowledge and Food Consumption Patterns and the Nutritional Status of Al-Ukhuwah Islamic Kindergarten Teachers in Tangerang
Abstrak: A teacher's health plays an important role in the teaching and learning process. Various kinds of nutritional problems in teachers or adults can occur, one of which is obesity. This research uses a cross-sectional study design. The subjects in this research were 26 Islamic Kindergarten teachers A;-Ukhuwah Tangerang. Subjects were drawn using purposive sampling. Data on the subject's food consumption patterns was determined using the Food Frequency Questionnaire (FFQ) and the level of nutritional adequacy, amount and type of food consumed using 2x24 hour food recall. For data processing and analysis, Microsoft Excel 2013 and the SPSS 16.0 for Windows were used. Energy adequacy is 30.8%, moderate deficit, excess protein and fat is 34.6% and 50%. Twelve of the 26 subjects were declared to be severely obese. There was no correlation between food consumption patterns (staple food (r=0.138), side dishes (r=0.363), vegetables (r=0.319), and fruit (r=0.355)) with the subject's nutritional status. The level of nutritional adequacy is also not related to nutritional status. This can be seen from the p-value of each level of sufficiency, namely energy (p=0.358), protein (p=0.627), fat (p=0.985) and carbohydrates (p=0.993). Keywords: Nutritional status, nutritional knowledge, food consumption pattern
Keyword: status gizi

Judul: Correlation between Nutritional Knowledge and Food Consumption with Nutritional Status of Primary School Children at Teluk Batang Village, Kayong Utara District
Abstrak: Anak-anak merupakan kelompok yang rentan mengalami masalah gizi. Salah satu faktor yang berpengaruh langsung terhadap status gizi anak adalah konsumsi pangan. Tujuan penelitian ini adalah mengkaji hubungan pengetahuan gizi dan kualitas konsumsi pangan dengan status gizi siswa Sekolah Dasar di Desa Teluk Batang, Kabupaten Kayong Utara. Penelitian ini menggunakan desain potong lintang dengan jumlah contoh penelitian sebanyak 60 orang, terdiri atas 19 orang siswa SD Negeri 01 Teluk Batang dan 41 orang siswa MIS Babul Mu’minin. Data diperoleh melalui pengisian kuesioner, recall 2×24 jam, dan pengukuran antropometri. Data dianalisis menggunakan Statistical Package for Social Science (SPSS) version 16 for Windows. Hasil uji korelasi menunjukan bahwa pengetahuan gizi contoh, pengetahuan gizi ibu, dan kualitas konsumsi pangan tidak memiliki hubungan yang signifikan (P>0,05) dengan status gizi. Namun, tingkat kecukupan protein memiliki hubungan yang signifikan negatif dengan status gizi contoh (P<0,05)., The susceptible group to suffer nutritional problems is children. One factor that directly influences nutritional status is food consumption. This study aimed to examine the correlation of nutrition knowledge, quality of food consumption and nutritional status of primary school children at Teluk Batang Village, Kayong Utara District. The design of this study was cross-sectional using primary data collected by purposive sampling. The total subject of this study was 60 subjects, consisting of 19 SD Negeri 01 Teluk Batang students and 41 MIS Babul Mu’minin students. Data collected using questionnaires, recall 2×24 hours, and anthropometric measurements. Data were analyzed by Statistical Package for Social Science (SPSS) version 16 for Windows. The results showed that nutritional knowledge of subject’s, mother’s nutritional knowledge, and quality of food consumption were not significantly correlated with nutritional status (P>0,05). However, adequacy level of protein significantly correlated with nutritional status of subjects (P<0,05).
Keyword: food, consumption, nutritional, knowledge, nutritional, status

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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