Judul: Biotyping Isolat Lokal Enterobacter sakazakii (Cronobacter spp.) dan Keragamana Genetik Beberapa Isolat Berdasarkan Gen infB
Abstrak: E. sakazakii was initially described as E. cloacae with yellow pigment, and later were classified based on their biochemical reactions, antibiotics susceprability and DNA-DNA hybridization. In 2007, E. sakazakii was reclassified into Cronobacter spp. consisting of C. sakazakii, C. malonaticus, C. muytjensii, C. dublinensis and C. turicensis based on f-AFLP and production of acid from dulcitol, production of indole, malonate utilization and production of acid from methyl-α-D-glucoside. All local isolates obtained from powder infant formula, weaning foods, starch, flours and spices in Indonesia have been identified as Cronobacter spp. and were reported to have close genetic relatedness based on its partial gene sequence encoding for 16S rRNA. However, they have been reported to have different thermal resistance (Seftiono 2012). This study aims to identify and classify local isolates of C. sakazakii based on their biochemical properties and genetic relatedness based on infB genes which known thermal kinetics. Identification of the 19 local isolates using RapID onE suggested that 9 isolates were identified as E. sakazakii, 9 others were E. cloacae and 1 was E. cancerogenus. When the results were compared to the identification using API 20E (Gitapratiwi 2011; Hamdani 2012), 8 of the 19 isolates were identified as E. sakazakii by both kits. Based on classification using 4 biochemical reaction, 15 isolates can be identified as Cronobacter spp. whereas 4 isolates can not be identified. By evaluating biochemical reaction in the RapID onE test kit, it can be conclude that reaction of pyrohydonyl-β-naphthylamide can be used as the additional testing to classify the previously unclassified local isolates into C. sakazakii. Ten of the 19 biochemical tests in RapID onE were selected for the biotyping of Cronobacter spp local isolates since they give different result for all isolates studied. Based on the ten biochemical profiles, the 19 local isolates of Cronobacter spp. can be classified into 16 biotypes. When the results of biotyping with rapid kit RapID onE was compared to result with the four biochemical reaction by Iversen, biotype I-VIII is C. sakazakii; while biotype IX, X, XV and XVI is C. malonaticus; biotype XIII and XIV is C. muytjensii; and biotype XI, XII and XVI is C. turicensis. v Genotypic characterization of infB gene among local isolates showed that all tested isolates where closely related. Based on the BLAST program, it was revealed that the contig sequence of the infB genes was as a translation initiation factor IF2. Based on the infB gene the local isolates can be classified into 2 groups. Isolat DES b7a, DES b10, DES c13, YR t3a and E6 belong to C. sakazakii, while isolate YR c3a is C. malonaticus and C. dublinensis.
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Judul: Skrining Gen Virulensi pada Isolat Lokal Cronobacter sakazakii.
Abstrak: Cronobacter sakazakii merupakan bakteri patogen emerging pada pangan yang dapat menyebabkan penyakit meningitis, sepsis, dan enterokolitis nekrotikan pada kelompok bayi tertentu yang telah dihubungkan dengan konsumsi susu bubuk formula. Bakteri tersebut telah diisolasi di Indonesia, meskipun sampai saat ini belum pernah dilaporkan kasus penyakit terkait infeksi C. sakazakii. Kemampuan bakteri ini dalam menyebabkan infeksi dikaitkan dengan beberapa gen virulensi. Penelitian ini bertujuan untuk mengetahui keberadaan gen-gen virulensi ompA, hfq, dan cpa pada beberapa galur isolat lokal C. sakazakii. Penelitian ini dilakukan melalui beberapa tahap, yaitu konfirmasi dan persiapan kultur dari isolat lokal C. sakazakii, ekstraksi DNA, amplifikasi gen virulensi dengan Polymerase Chain Reaction (PCR), serta elektroforesis gel agarose dan pewarnaan DNA dengan menggunakan etidium bromida untuk visualisasi sebagai konfirmasi keberadaan gen-gen virulensi yang telah disebutkan. Hasil penelitian menunjukkan bahwa kesepuluh isolat lokal C. sakazakii berpotensi patogen karena memiliki gen-gen virulensi ompA dan hfq. Sementara, 9 (sembilan) dari 10 (sepuluh) isolat juga memiliki gen virulensi tambahan cpa.
Keyword: cpa, Cronobacter sakazakii, hfq, ompA, PCR, virulensi

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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