Judul: Pengembangan Pertanian Organik di Kelompok Tani Madya Desa Kebonagung, Kabupaten Bantul, Daerah Istimewa Yogyakarta
Abstrak: Organic farming is agricultural activities that seek the use of outside intake and avoid the use of pesticides and synthetic fertilizer. The results showed that the organic farmers and conventional farmers have the high responses of organic farming. However, the characteristics of organic farmer have no relation with farmer’s respons of organic farming. Then, in conventional farmers, formal education and the courage to take the risks have relation with farmer’s respons of organic farming. This research also showed there are no relation between farmer’s respons of organic farming with farmer’s income. Nevertheless, can be predicted that there are chances of the conventional farmers to adopt the organic farming
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Judul: Penerapan Teknologi Pertanian Padi Organik Di Kampung Ciburuy, Desa Ciburuy, Kecamatan Cigombong, Kabupaten Bogor
Abstrak: The objectives of this study are to identify the implementation of organic farming, identify farmer’s perception about the characteristic of organic farming innovation, analyze the influence of farmer’s perception to their farming implementation, and also to analyze the influence of farmer’s characteristic to their perception about organic farming innovation. The unit of analysis in this study are individuals, such as, farmers who assumed to make rational decision before deciding to do farming activities. Population was all farmers in Ciburuy village. Study respondents are the owner and tenant farmers that selected by using simple random sampling. The conclusion of this studies are (1) Farmer’s positive perception has a tendency to higher percentage than farmer’s neutral perception of organic farming. This suggests that the implementation of organic farming was influenced by farmer’s perception. (2) In the broad-land area category, has a tendency to higher percentage than narrow-land area. This suggests that farmer’s perception about the characteristic of organic farming innovation was influenced by farmer’s broad-land area. (3) Farmers who dare to take a middle risks, has a tendency to neutral perception of organic farming innovation. This suggest that farmer’s perception about the characteristic of organic farming innovation was influenced by farmer’s courage to take a middle risks. (4) In innovative categories, farmer’s neutral perception has a tendency to higher percentage than less-innovative categories. This suggests that farmer’s perception about the characteristic of organic farming innovation was influenced by farmer’s innovativeness. (5) In farmer’s categories about highly exposed to information, has a tendency to higher percentage than exposed to information categories. This suggests that farmer’s perception about the characteristic of organic farming innovation was influenced by farmer’s ability to expose the information. (6) In farmer’s cosmopolite categories, has a tendency to higher percentage than farmer’s not cosmopolite categories. This suggests that farmer’s perception about the characteristic of organic farming innovation was influenced by farmer’s ability to live out from their birth place.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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