Judul: Variasi Alel dari Gen 5-HTT (Penyandi Agresivitas) pada Macaca nemestrina
Abstrak: Macaca nemestrina is an aggressive primate. The aggresivity is influenced by many factors, one of them is by a gene. The 5-HTT gene encodes the serotonin transporter that affects the regulation of serotonergic system and its receptor by modulating the concentration of serotonin in extracellular fluid. Variation in promoter and intron region can change the transcriptional activity of the 5-HTT gene, mainly the activity of the transporter and serotonin uptake. The transporter change activity influenced aggressivity, emotion, motor function, and some cognitive traits in primates. This research aimed to determine alelles variant of 5-HTT gene (encoding aggresivity) in M. nemestrina. Samples used in this research consisted of 56 M. nemestrina. The gene was amplified by the Polymerase Chain Reaction (PCR) method. Amplification in promoter region revealed two alleles and three genotypes with the frequencies of L and S alleles were 88,18% and 11,82%, respectively. Amplification of the third intron region showed two alleles with the frequency of l and s alleles were 55,4% and 44,6%, respectively. None of the individual had s/s genotype in the third intron region. Short allele was dominant to long allele. The presence of at least one short allele decreased transcription activity. There was one individual with S/S genotype in promoter region that was possibly to be the alpha male in the group.
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Judul: Keragaman Gen Monoamine  Oxidase A (MAOA) pada Sapi Pedaging
Abstrak: One of the essential traits in the management of raising meat livestock is the aggressive nature of cattle, which affects livestock handling and productivity. Several genes control aggressive traits, and one that plays an important role is the monoamine oxidase A (MAOA) gene. The MAOA gene encodes the enzyme monoamine oxidase A, which plays a role in the catabolism of neurotransmitters, including dopamine, norepinephrine, and serotonin. Mutations in the MAOA gene can increase the tendency of cows to behave aggressively, which can affect neurotransmitter regulation. Studies on MAOA gene diversity in Indonesian beef cattle have yet to be carried out. Therefore, this research was conducted to identify and analyze SNP determination and MAOA gene diversity in beef cattle in Indonesia. One hundred twenty-seven blood samples from beef cattle were from Bali, Limousin, Wagyu, PO, Madura, and Balinese Wagyu crosses. Blood samples were extracted using the GeneAid kit, isolating pure DNA from beef cattle. MAOA gene diversity was analyzed using the PCR-RFLP method. Analysis results such as genotype frequencies, allele frequencies, heterozygosity values, and Hardy-Weinberg balance were calculated using Popgen32. Amplification of the MAOA gene sequence in the promoter fragment and exon 1 via PCR was successfully carried out at 57 ºC, resulting in product lengths of 548 bp and 341 bp, respectively. The results showed that the MAOA gene located in the promoter region has six SNPs that are specific to Bali cattle, namely SNP g.98 A > C, SNP g.145 G > T, SNP g.216 A > C, SNP g.340 T > G, SNP g.349 A > C, and SNP g.385 G > A. Meanwhile, the MAOA gene located in exon 1 is monomorphic. The PCR-RFLP method was used to investigate the SNP g.385 G > A MAOA gene polymorphism using the RSaI restriction enzyme. The MAOA SNP g.385 G > A gene obtained two alleles (G and A) and three genotypes: GG, GA, and AA. SNP g.385 G > A is polymorphic in Bali, PO, Madura, and Wagyu-Bali cross (F1) cattle while monomorphic in Limousin and Wagyu cattle. The allele frequency of the SNP g.385 G > A MAOA gene in Bali, PO, Madurese cattle, and Wagyu-Bali crosses (F1) is in Hardy-Weinberg equilibrium. SNP g.385 G > A of the MAOA gene can be used as a candidate aggressive genetic marker., Salah satu sifat yang penting dalam manajemen pemeliharaan ternak daging adalah sifat agresif pada sapi yang berpengaruh pada penanganan ternak dan produktivitas. Sifat agresif dikendalikan oleh beberapa gen dan salah satu yang berperan penting adalah gen MAOA. Gen MAOA mengkodekan enzim monoamine oksidase A yang berperan dalam katabolisme neurotransmiter, termasuk dopamin, norepinefrin, dan serotonin. Mutasi pada gen MAOA dapat meningkatkan kecenderungan sapi untuk berperilaku agresif yang dapat mempengaruhi regulasi neurotransmitter. Studi keragaman gen MAOA pada sapi pedaging Indonesia belum dilakukan. Oleh karena itu, penelitian ini dilakukan untuk mengidentifikasi dan menganalisis penetuan SNP dan keragaman gen MAOA pada sapi pedaging di Indonesia. Sebanyak 127 sampel darah sapi pedaging yang terdiri dari sampel darah sapi Bali, Limousin, Wagyu, PO, Madura, dan silangan Wagyu Bali. Sampel darah diekstraksi menggunakan kit GeneAid dan
Keyword: beef cattle, MAOA gene, SNP

Judul: Perencanaan Desain Embung di Kampus IPB Darmaga Bogor
Abstrak: Kampus IPB Darmaga membutuhkan sumber daya air alternatif untuk mencegah terjadinya kekurangan air akibat perubahan musim yang tidak menentu. Oleh karena itu tujuan dari penelitian ini adalah merancang embung sebagai penyimpan air hujan serta menghitung anggaran biayanya. Embung dirancang di lahan kosong antara kawasan Fakultas Peternakan dan Fakultas Kedokteran Hewan IPB dengan bentuk sesuai kontur tanpa penggalian serta pemadatan. Berdasarkan hasil pemetaan, bentuk embung mengikuti garis kontur pada elevasi 190 mdpl dengan kedalaman 2 m dan volume tampungan embung sebesar 2089 m3. Pelimpah berupa urugan batu yang dilapisi geomembran atau kanvas beton dengan lebar outlet 2 m dan tinggi 0.10 m. Embung dengan lapisan geomembran lebih efektif dari dengan lapisan kanvas beton, karena hanya membutuhkan dana sekitar Rp 1.7 M dan waktu pembangunan 46 hari. Biaya pembangunan dengan kanvas beton lebih mahal karena materialnya belum diproduksi di Indonesia.
Keyword: desain, embung, geomembran, kanvas beton, pelimpah