Judul: ultur In Vitro Sel-sel Otak Besar (Cerebrum) Anak Tikus
Abstrak: Research has been conducted on in vitro culture of three days old rat cerebrum cells in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% Fetal Bovine Serum (FBS) and 50 μg/ml gentamycin, and supplemented with and without 5 μg/ml insulin, 10 μg/ml transferin, 5 μg/ml selenium (ITS). Culture was done in 5% CO2 incubator at 37oC for 11 days. Identification was done on types of cell based on morphological observation, growth capacity (cell proliferation, population doubling time/ PDT and length of axon and dendrit) based on calculation of the number of cells using hemocytometer and micrometer, and the protein produced by the cerebrum cells in the culture medium using sodium dodecyl sulfate–polyacrilamide gel electrophoresis (SDS-PAGE) method. Quantitative data were analyzed using statistical T-test on Minitab program. The results showed that based on the morphological observations, there are two types of cells including nerve cells of bipolar and multipolar neurons and glial cells including the fibrous astrocyte, protoplasmic astrocyte, oligodendrocyte, and microglia. Supplementation of ITS into the culture medium could increase the cells proliferation rate (P<0.05) with lower PDT, axon and dendrit lenght growth and quantitatively the 21.5–36.5 kDa protein production as indicated by the intensity of the protein band.
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Judul: Kultur in vitro sel-sel fibroblas fetus tikus
Abstrak: Penelitian yang dilakukan berupa kultur in vitro sel-sel fibroblas fetus tikus menggunakan Dulbeccos’ Modified Eagle Medium yang dimodifikasi dengan penambahan 3mM natrium bikarbonat, 10% fetal calf serum, dan 50 μg/ml gentamisin (mDMEM) di dalam inkubator CO2 pada suhu 37ºC. Evaluasi dilakukan terhadap tingkat proliferasi dan produksi protein setelah beberapa pasase. Pertumbuhan sel-sel fibroblas diidentifikasi melalui pengamatan morfologi. Tingkat proliferasi sel ditentukan berdasarkan population doubling time (PDT) dengan menghitung jumlah sel pada setiap pasase menggunakan hemositometer Improved Neubauer. Identifikasi protein dilakukan dengan metode Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Berdasarkan pengamatan morfologi, sel yang berkembang di dalam kultur in vitro adalah sel fibrosit dan sel fibroblas dan memperlihatkan peningkatan homogenitas setelah beberapa pasase. Berdasarkan nilai PDT, tingkat proliferasi sel juga meningkat seiring pasase. Sel fibroblas juga tetap mensekresikan protein hingga pasase 9 berdasarkan hasil elektroforesis. Dengan demikian dapat disimpulkan bahwa pertumbuhan dan sekresi protein dari kultur in vitro sel-sel fibroblas bisa dipertahankan sampai 9 pasase.
Keyword: Kultur sel, Fibroblas, Bogor Agricultural University

Judul: Impact Of water policy on poverty reduction and women roles in water management In Zimbabwe
Abstrak: Water management is a key factor in the battle to remove the scourge of extreme poverty and to build secure and prosperous lives for people in the developing world. The water sector in Zimbabwe is complicated by the large number of actors; it is interwoven within various ministries. The research used secondary data and review of literature to evaluate how the current water policy has contributed towards marginalising women and the poor. It looked at how actors can also marginalise or put other actors at risk thereby undermining their role through prioritisation of certain projects, stakeholder participation and usage policy. From a descriptive analysis it is found that women are further relegated and marginalised. The poor are further impoverished through marginalisation and vulnerability. The policy environment favours business and state interests against those of grassroots actors and women. It recommends the establishment of home grown pro- poor water management strategies.
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