Judul: Pengaruh pupuk kotoran ayam, babi dan kambing dengan intensitas cahaya yang berbeda terhadap perkembangan aufwuchs di dalam bak-bak teraso
Abstrak: Penelitian nengaruh buruk kotoran ayam, babi dan kambing dengan intensitas cahaya yang berbeda terhadan perkembangan aufwuchs telah dilaksanakan dari tanggal 23 September sampai dengan 7 November 1979 dalam bak-bak teraso di Fakultas Perikanan IPB. Dalam penelitian ini digunakan 16 buah bak yang masing-masing berukuran menjeng, lebar dan tinggi 75,6 x 50,5 3 x 1,5 cm. Fenempatan bak dibagi delam dua kelompok yaitu kelompok yang mendapat cahaye langsung diletakkar di luar ruangan sedangkan kelompok yang tidak mendapat cahsya langsung diletakkan didalam labolatorium yang beratankan plastik. Rancangan vercobaan yang digunakan adalah Faktorial dengan pola rancangan Acak lengkap. ...
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Judul: Pengaruh bentuk fisik makanan dan penambahan cahaya terhadap performans ayam pedaging
Abstrak: Penelitian ini dilakukan di Laboratorium Ilmu Teernak Unggas, Jurusan Ilmu Produksi Ternak, Fakultas Peternakan, Institut Pertanian Bogor, dimulai dari tanggal 15 Februari sampai dengan tanggal 4 April 1984. Tujuan penelitian untuk mengetahui pengaruh bentuk fisik makanan dan penambahan cahaya terhadap performans ayam pedaging. Pada penelitian ini digunakan 180 ekor ayam pedaging strain "C.P. 707" jenis kelamin campur. Anak ayam sampai umur 3 minggu dilakukan pergantian ransum "starter" dengan ransum "finisher", sekaligus dimulai perlakuan.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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