Judul: Desain Taman Sekolah Pendidikan Anak Usia Dini (PAUD) Gema Imani Bogor
Abstrak: Early childhood education is the lowest level education for children of 0-6 years. In this phase, education influence child development. Therefore, educational facilities must support child development. Outdoor facilities are facilities that can made children to learn about nature. Outdoor facilities include a garden designed according to national and international standards to fulfill early childhood education curriculum. Garden design is divided into two main area, front garden and rear garden. Rear garden focused on children's games that use equipment such as swings, slides, balance beam, arch climbers, rope climbing, etc. The lawn in front garden accommodate free activity such as running, walking, jumping, etc
Keyword: Children growth, Curicculum, Design early-childhood school yard

Judul: Garden School Design to Support Teaching and Learning Activities at SLB Negeri 1 Kota Bogor
Abstrak: SLB Negeri 1 Kota Bogor adalah sekolah khusus yang menyediakan layanan pendidikan bagi anak-anak tunanetra, tunarungu, autisme, dan disabilitas. Kurangnya fasilitas dan aksesibilitas masih menjadi masalah dalam kegiatan belajar mengajar di SLB Negeri 1 Kota Bogor. Fasilitas belajar untuk anak berkebutuhan khusus dapat didukung oleh taman sekolah. Taman dapat menjadi ruang rehabilitasi, ruang kerja, dan ruang belajar bagi anak berkebutuhan khusus. Penelitian ini bertujuan untuk menganalisis kebutuhan pengguna taman, mengembangkan konsep, dan merancang taman sekolah untuk mendukung kegiatan belajar mengajar di SLB Negeri 1 Kota Bogor. Metode yang digunakan adalah pendekatan desain berdasarkan aktivitas pengguna. Aktivitas pengguna diperoleh dari observasi langsung, kuesioner, dan wawancara, kemudian data diolah melalui analisis deskriptif. Ditemukan bahwa kondisi tapak saat ini masih belum memenuhi kebutuhan siswa, terutama dari aspek keamanan, kenyamanan, dan orientasi. Taman yang ada masih belum digunakan sebagai ruang untuk menunjang kegiatan pembelajaran sehingga perlu dibuat ruang-ruang dan fasilitas penunjang pembelajaran seperti fasilitas terapi. Keamanan masih belum terpenuhi karena kondisi topografi yang curam, sehingga perlu dibuat jalur sirkulasi yang mengikuti topografi. Aspek kenyamanan masih belum terpenuhi karena jalur sirkulasi belum sesuai dengan standar, sehingga perlu dibuat jalur sirkulasi yang sesuai dengan standar berlaku untuk mempermudah orientasi siswa. Konsep yang diangkat adalah empat pilar pembelajaran dari UNESCO yang menekankan eksplorasi dalam belajar. Desain akhir dibuat dengan pola organik yang menyesuaikan kondisi topografi tapak dan mempertimbangkan kebutuhan, kenyamanan, serta keamanan pengguna.
Keyword: Landscape Design, school garden, special education, special needs children, therapeutic garden

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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