Judul: Variasi Wajah dan Penderita Hipertensi pada Wanita di Kampung Naga
Abstrak: Wajah berguna untuk membedakan manusia yang satu dengan yang lain. mengetahui ekspresi dan erosi seseorang, serta komunikasi non-verbal. Bentuk wajah seseorang sering dikaitkan dengan suatu penyakit tetapi balum diketahui kebenarannya, misalnya hipertensi. Hipertensi dikategorikan sebagai the silent disease dengan jumlah penderita di Asia mencapai 19,6%. Kampung Naga merupakan perkampungan dengan pola perkawinan yang bersifat endogami sehingga variabilitas hayati kecil dan spesifitas suku dapat dipertahankan. Penelitian ini bertujuan untuk mengetahui variasi wajah dan penderita hipertensi pada wanita di Kampung Naga. Subyek yang digunakan adalah wanita Kampung Naga yang berusia 14-92 tahun (rata-rata 43,54 tahun).
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Judul: Variasi wajah pria Kampung Naga
Abstrak: Keragaman morfologi dapat diukur dengan menggunakan koordinat dari bagian-bagian yang ada di dalam satu bentuk. Data dari pengukuran ini dapat dianalisis dengan perangkat geometri "Thin Plate Spline" yang dikembangkan oleh Bookstein (1991). Perangkat geometri ini mampu menggambarkan pergerakan titik-titik anatomis dari suatu bentuk ke bentuk lainnya.
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Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.