Judul: Pengaruh Sistem Perakaran Dan Jenis Bibit Terhadap Pertumbuhan Bibit Duku (Lansium domesticum var. domesticum)
Abstrak: Penelitian ini bertujuan untuk membandingkan pertumbuhan bibit duku dengan sistem perakaran tunggal dan sistem perakaran ganda serta pertumbuhan bibit seedling, bibit grafting entres juvenil, dan bibit grafting entres dewasa. Penelitian dilaksanakan di rumah plastik Kebun Percobaan Tajur IPB yang terletak pada altitude 250 meter dari permukaan Iaut dari bulan Maret sampai Desember 2001
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Judul: Pengaruh Media Tumbuh Terhadap Pertumbuhan Duku (Lansium domesticum Corr.)
Abstrak: Percobaan ini dilakukan untuk mencari komposisi media tanah, pasir dan bahan organik terbaik bagi pertumbuhan bibit tanaman duku. Percobaan dilakukan di Kebun Percobaan IPB Tajur pada bulan 5uli 2001 sampai dengan bulan November 2001. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan satu faktor. Faktor yang diuji dalam penelitian ini adalah perlakuan komposisi media Tanah : Pasir: Bahan Organik (v/v/v) yang terdiri dari 12 taraf yaitu: Komposisi Tanah : Pasir : Pupuk Kandang Sapi dengan perbandingan 3 : 2 : 1,2 : 2 : 1 dan 2 : 3 : 1. Komposisi Tanah : Pasir : Kasting dengan perbandingan 3 : 2 : 1. 2 : 2 : 1 dan 2 : 3 : 1 . Komposisi Tanah : Pasir : Kompos Daun Bambu dengan perbandingan 3 : 2 : 1, 2 : 2 : 1 dm 2 : 3 : 1. Komposisi Tanah : Pasir : Green Leaf 300 dengan perbandingan 3:2:1, 2:2:1 dan 2:3 :1
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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