Judul: Hubungan Kekerabatan Beberapa Isolat Lentinus Asal Tropis Berdasarkan Sifat Mikroskopik Kultur dan Molekular
Abstrak: Jamur Lentinus merupakan jamur pelapuk kayu yang berpotensi sebagai obat yang tersebar di berbagai kawasan daerah tropis dan subtropis. Penelitian ini bertujuan untuk mengetahui hubungan kekerabatan jamur Lentinus isolat tropis berdasarkan sifat mikroskopik kultur dan sifat molekular. Sampel yang digunakan ialah Lentinus isolat LU3, LU10 (L. torulosus), LC4, LC6 (L. cladopus), LSC1, LSC7 (L. Sajor-caju), LP9 (L. quarrosulus), dan HS serta JTP (Pleurotus). Media yang digunakan adalah agar ekstrak malt pepton (AEMP) dan agar ekstrak malt (AEM) pada suhu 35°C untuk isolat Lentinus dan ±29°C untuk isolat HS dan JTP. Struktur mikroskopik kultur setiap isolat diamati setelah isolat berumur 2 dan 4 minggu. Analisis molekular dilakukan dengan mengamplifikasi DNA berdasarkan metode PCR-RAPD dengan menggunakan 2 primer acak tunggal 10-mer, yaitu RP1 dan RP3. Sifat mikroskopik kultur dan molekular dinyatakan ke dalam data biner dan diolah dengan program MEGA 4.1. Hasil pengamatan menunjukkan bahwa beberapa isolat Lentinus yang ditumbuhkan pada media AEMP memiliki penampakan koloni yang berbeda dengan penampakan koloni pada media AEM. Berdasarkan pohon kekerabatan mikroskopik kultur dan molekular, isolat Pleurotus (JTP) berada di luar kelompok A (kelompok Lentinus dan HS) sebagai out group. Kelompok A terbagi menjadi dua cabang yaitu HS dan kelompok Lentinus (B, C, D). Berdasarkan sifat molekular kelompok Lentinus terdiri dari kelompok B dengan nilai konsistensi (NK) 57% yang terdiri dari LP9 dan LSC1 (NK=89%) dan kelompok C dengan NK 20%. Kelompok C terdiri dari LSC7 dan LC6 (NK=65%) dan kelompok D dengan NK=45%. Kelompok D terdiri dari isolat LU10 dan isolat LC4 dan LU3 (NK=46%). Hubungan kekerabatan Lentinus berdasarkan molekular belum spesifik sehingga perlu dianalisis kembali dengan penambahan primer lain.
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Judul: Analisis Keanekaragaman Isolat Pleurotus spp. Liar dan Domestik Berdasar Sifat Morfologi, Fisiologi dan Molekuler
Abstrak: Oyster mushroom (Pleurotus spp.) are edible mushroom with high diversity. Cultivated oyster isolates in Indonesia are from local isolates as well as from outside Indonesia. Report on their genetic diversity based on characteristic of morphological and physiological are still scare found in Indonesia, even analytic based on molecular characteristic is never report in Indonesia. This study was aimed to identify several kind of wild and domestic isolates of oyster mushrooms based on morphological, physiological, genetic, and molecular characteristic. The result indicated that there were two groups of isolates based on morphological characteristic of a fruiting body, A group and B group. A group was consisted ofHHB247 isolate (pink color) and nine isolates (white and brown color) grouped on B which then divided into two groups B1 and B2_ This B1 divided into two groups C1 and C2. C1 included only one isolate that was HHB215 isolate (brown color ) and C2 include one white color isolate (sp2) and one brown color isolate (sp6). B2 divided two groups D1 and D2, D1 included two white color isolates (sp3, sp5) and one brown color isolate (HHB328). D2 included three white color isolates (HHB327, sp4 and spl). Based on morphological characteristic the, ten of oyster mushroom isolates can not be in groups related to the color of fruiting bodies. The result indicated that there were two groups of isolates based on physiological characteristic, A group and B group. A group was consisted of HHB247 isolate (pink color) and nine isolates (white and brown color) grouped on B which then divided into two groups B1 and B2. B1 included only one isolate that was sp6 isolate (brown color). B2 divided two groups Cl and C2. Cl included two brown color isolates (HHB215, HHB328), C2 divided two groups Dl and D2. Dl include five white color isolates (spl, sp2, sp3, sp4, and sp5), D2 included only one isolate that was HHB327 isolate (white color). Based on physiological characteristic, ten of oyster mushroom isolates can be in groups related to the color. The frequency of mating between single spores of intra isolates (similar color of fruiting bodies) was highly (60% - 74%), but the frequency of mating between inter isolates was O %. Molecular analysis on Pleurotus spp. and Lentinus isolates was done using RAPD molecular marker with five primers (RP-2, RP-3, Rp-3, RP-4 and RP-6). The result indicated that there were two groups of isolates based on moleculer characteristic, A group and B group. A divided two groups Al and A2. Al included only one isolate that was HHB307 isolate ( the color was not determined), and A2 included six white color isolates (HHB327, Spl, Sp2, Sp3, Sp4, and Sp5) with 100% of similarity index. B divided two groups Bl and B2. Bl included three brown color isolates (HHB32S, HHB215 and sp6) with two last isolates had 100% of similarity index, and B2 included one...dst
Keyword: jamur tiram, RAPD, mating, analisis molekuler, spora tunggal

Judul: Rancang Bangun Pendeteksi Otomatis Ketinggian Permukaan Air Berbasis Sensor Medan Magnet UGN3503
Abstrak: An automatic detector of water level with sensor UGN3505 as detector of changes water level has been successfully be made for this research. Sensor UGN3505 is a hall effect sensor which works based on the large magnetic fields on the surface. The output voltage generated by the sensor without the influence of a magnetic field is 2,525 volts, the output voltage is different than it should be 0.025 volts is 2.50. This shows that the sensor is not completely free from magnetic field sources, there is still the effect of the Earth's magnetic field to the sensor. Sensor output voltage will increase, with increasing magnetic field on the surface of the sensor. As the central data processing equipment, used microcontroller ATmega16. The input of microcontroller is the changes output voltage of sensor than has been converted into digital data (internal ADC ATmega16) caused the changes of magnetic field. The digital data are readable by microcontroller be processed and displayed in the 16x2 LCD, LED indicator and buzzer. Programs created using software tools CVAVR with C + + language.
Keyword: Sensor UGN3505, magnetic field, hall effect, CVAVR, ATMega16, DC