Judul: The Promotore and Intron Nucleotide Variation of Myostatin Gene within Indonesiau Local Sheeps
Abstrak: lvIyostatin or growth and di/Terentiation Jactor 8 (GOF-8) is a member of transforming growth Jactor-il (TG f.-il) superfamily of growth and differentiation factors. GOF-8 is essential for proper regulation of skeletal muscle mass in vertebrate, that is expressed specifically postnatal. In mutations condition, it is suggested that gene to be a "loss of function" which give an effect to increasing skeletal mass, due to the increasing number of muscle tlbres (hypelplasia) or the size of muscle fibres (hypertrophy). Some mutation that occurs not in a loss of function, might be improve their ability to promote muscle growth or even in decreasing muscle size. The PCRSSCP technique was used to identify of nucleotide mutation in promotor region and intron 2 of myostatin gene of some local sheep in Indonesia. A total of 145 blood samples were used in this study comprised four localities, i.e. Cibanteng (43), Cilebut (26), UP3 Jonggol (38) and Wanaraja (38). The result showed that there are four types of myostatin gene based on T'CR-SSCP (type K, L, lvI and N) promoter region. The K and lvI types were found in all population, however the Land N types were not found in Wanaraja and Cilebut population respectivly. In the region of intron 2 we found five defer<;nt types of gene. There are gene type of A, E, C, F and G. The A type gene from intron 2 found only in Cibanteng population. From sequencing data, K, lvi, and N gen type were found from the promotor area. There are 8 mutation points in gen type K, 4 mutation points in gen type lvi, and 34 mutation points in gen type N. However, the gene types did not correlate whith the growth rate of the sheep. Nevertheless, the mutated gene described the polymorphism of Indonesian local sheep.
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Judul: Identifikasi Keragaman Gen Miostatin pada Domba Lokal
Abstrak: Myostatin is a member of tranforming grown factor B superfamily, know as growth and differentiation factor, which is essensial for proper regulation of skeletal muscle mass in wide range vertebrate species. To pursue potential agricultural application of increasing muscle mass by inhibition of myostatin activities, the aim of this study was to identify the variants of myostatin gene in the Indonesian local sheeps. A total of 151 DNA samples belonging to eight different areas that are Bogor Ciomas (20), Bogor Jonggol (35), Margawati (18), Indramayu (30), Dinggala (24), MAdura (8), Sumbawa (8), and Rote (8) were analyzed with Polymerase Chain Reaction-Single Starnd Conformation Polymorphism. The Myostatin (GDF 8) amplified product was 688 bp(base pair)
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Judul: Pengaruh panjang deret waktu data terhadap perhitungan debit banjir rencana
Abstrak: Dalam perencanaan bangunan air, harus ditunjang oleh data yang lengkap. Pada kenyataannya data yang lengkap dewasa ini sulit diperoleh. Selain itu pengambilan data ini memerlukan waktu, ketepatan, ketelitian serta biaya yang cukup besar. Tujuan dari penelitian ini adalah mempelajari pengaruh panjang data terhadap perhitungan banjir rencana, menentukan besarnya banjir maksimum yang akan terjadi untuk periode waktu yang berbeda, serta menentukan distribusi banjir teoritis yang paling sesuai pada daerah yang diteliti. Menurut Chow (1964), di dalam analisa hidrologi digunakan asumsi bahwa kejadian-kejadian selama pengamatan cukup mewakili dan hasil pengamatan tersebut dapat dipercaya. Kepercayaan semakin meningkat dengan bertambah lamanya pencatatan data. Dalam kenyataannya, lama pencatatan data yang dilakukan tergantung dari periode ulang yang akan digunakan dalam mendesain suatu bangunan. Menduga suatu kejadian dengan periode ulang yang lebih besar daripada periode pencatatan menyebabkan hasil pendugaan tidak dapat dipercaya....
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