Judul: Periode Pembungaan dan Aplikasinya dalam Taman
Abstrak: Trees is one of the vegetation that can be an attraction in landscape. Some trees show a strong character in color which is presented by flower. Nowadays, information and studies about the types of trees and flowering period is still limited. One of the studies that can be conducted to determine the time of flowering periode is phenology. This study aims to determine the flowering period of trees and the application in site design. The study was conducted for 6 months using 10 species of trees from the different types of families. This study used a grid method to count percentage of coverage flowers in the canopy and linear regression method to determine the relation between flowering periode and climatic factors. Based on the results, Samanea saman and Tabebuia caraiba showed there is a significant influence of climate factors to flowering percentage. The result of this study showed the flowering of S. saman is significantly influenced by climatic factors, such as maximum-minimum temperature difference, daylight, and frequency of rain. The increasing of the temperature difference between minimum and maximum and daylight tends to increase flowers percentage of S. Saman, whereas the increasing of percentage frequency of rain tend to decrease it. Flowers percentage of T. caraiba also significantly influenced by daylight. The increasing of daylight tend to increase it flowers percentage. The final product of this study is a recommendation of application of flowering trees in a site design.
Keyword: phenology, garden design, flowering tree, flowering period

Judul: Periode pembungaan pohon pada musim hujan dan aplikasinya dalam taman
Abstrak: Tanaman merupakan salah satu elemen utama pembentuk lanskap pada taman. Tanaman dapat dikategorikan menjadi tanaman penutup tanah, semak, perdu, dan pohon. Tiap kategori memiliki fungsi yang berbeda. Pohon memiliki fungsi sebagai pelindung, peneduh, display, dan pengarah yang berasal dari tinggi pohon, tekstur dan warna daun, bunga, dan bentuk tajuk pohon. Beberapa pohon menunjukan karakter kuat terhadap warna yang ditampilkan oleh bunga. Informasi dan studi terhadap jenis pohon serta periode pembungaan masih terbatas. Studi yang dapat dilakukan untuk mengetahui waktu pembungaan salah satunya adalah dengan studi fenologi. Penelitian ini bertujuan untuk mengetahui masa pembungaan pada pohon berbunga serta aplikasi penggunaannya dalam taman. Penelitian dilakukan selama 6 bulan dengan menggunakan 10 jenis pohon dari berbagai jenis famili. Penelitian ini menggunakan metode grid dari salah satu perangkat lunak (software) untuk menghitung persentase penutupan bunga pada tajuk sehingga mempermudah penelitian, serta menggunakan metode regresi linear untuk mengetahui hubungan antara periode pembungaan dengan faktor iklim. Berdasarkan hasil analisis, 3 dari 10 jenis pohon menunjukkan hubungan signifikan antara persentase pembungaan dengan faktor iklim, yaitu spesies Jacaranda acutifolia, Plumeria rubra dan Tabebuia caraiba. Hasil tersebut menunjukkan pembungaan J. acutifolia secara signifikan dipengaruhi oleh faktor iklim, yaitu kelembaban udara. Peningkatan nilai kelembaban udara cenderung meningkatkan persentase bunga. Peningkatan nilai selisih suhu maksimumminimum dan lama penyinaran cenderung meningkatkan persentase bunga, sedangkan peningkatan jumlah hari hujan dan kelembaban udara cenderung menurunkan persentase bunga Plumeria rubra. Hasil serupa juga ditunjukkan oleh Tabebuia caraiba. Pembungaan T. caraiba dipengaruhi secara signifikan oleh faktor iklim, antara lain selisih suhu maksimum dengan minimum, lama penyinaran, hari hujan, dan kelembaban udara. Hasil akhir dari penelitian ini berupa rekomendasi aplikasi penataan pohon berbunga dalam suatu desain taman.
Keyword: flowering period, flowering tree, garden design, phenology

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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