Judul: Identifikasi Gen Aroma Pada BC5F1 Ciherang-Pandan Wangi dan BC4F1 Ciherang-Mentik Wangi
Abstrak: The demand of high quality rice becomes increasingly in Asian market and around the world. The increase in productivity and quality of Indonesian agriculture has continued, such as improving new aromatic rice variety to have good characteristics (resistant to pest, ) which is like non-aromatic rice. The aim of this research was to develop new varieties of non transgenic aromatic rice by induction of aroma gene (mutated badh2) from aromatic varieties (Pandan Wangi or Mentik Wangi) to non aromatic varieties rice (Ciherang). Rice BC5F1 CP (Ciherang/Pandan Wangi) and BC4F1 CM (Ciherang/Mentik Wangi) that used in this research were the result from previous research with site-directed crossing method. Selection of plant was done by PCR with RM223 marker for CP and Bradbury marker for CM. Visualization of DNA sample by PCR was done by using gel agarose 3% for CP and 2% for CM. DNA amplification result of BC5F1 CP with RM223 marker can distinguish the band pattern of aromatic and non aromatic with band size 140 bp and 160 bp. In addition, DNA amplification result of BC4F1 CM with Bradbury marker produced DNA band size 580 bp, 355 bp and 257 bp. The succeed in introgression of aroma gene from Pandan Wangi or Mentik Wangi to Ciherang was showed by heterozygote band.
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Judul: Seleksi menggunakan PCR berdasarkan marka gen badh2 pada pembentukan BC2F1 Ciherang/Mentik Wangi dan BC3F1 Ciherang/Pandan Wangi
Abstrak: Commercial value and market demand (national and international) of aromatic rice is increasing. However, its less agronomy quality traits. This research is part of the development of new nontransgenic aromatic varieties with good agronomy character as nonaromatic rice through site-directed crossing. BC1F1 Ciherang/Mentik Wangi (CM) and BC2F1 Ciherang/Pandan Wangi (CP) obtained from previous studies were backcrossed with Ciherang to obtain progenies BC2F1 CM and BC3F1 CP. Selection were carried using Bradbury marker assisted PCR for CM and RM223 assisted PCR for CP. DNA samples were isolated from young leaves, PCR amplificated, and analyze using agarose 1.5% for CM and 3% for CP. Results showed heterozygous bands for both BC2F1 CM and BC3F1 CP, indicated succesful transfer of aroma badh2 heterozygous gene from Mentik Wangi or Pandan Wangi to Ciherang. DNA amplification result of BC2F1 CM using Bradbury marker obtained size of band about 580 bp, 355 bp, and 257 bp. Furthermore, RM223 marker can differentiate both the patterns of aromatic and nonaromatic rice by the band size about 160 bp and 140 bp.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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