Judul: Pendugaan Diffusitas thermal dan damping depth pada beberapa penutup lahan untuk menduga suhu udara menggunakan citra satelit terra/aster
Abstrak: Perbedaan penggunaan lahan sangat mempengaruhi kondisi iklim di suatu wilayah. Masing-masing lahan dengan pemanfaatan yangberbeda akan mempunyai karakteristik iklim yang berbeda jauh. Karakteristik unsur iklim yang paling jelas terasa pada berbagai jenis penggunaan lahan adalah suhu, baik itu suhu permukaan maupun suhu udara. Perbedaan suhu permukaan dan suhu udara antara dan penggunaan lahan yang satu dan penggunaan lahan lainya berkaitan erat denagn sifat penutup lahan tersebut terhadap penerimaan radiasi matahari, dan kemampuan bahan penutup lahan tersebut melepaskan panas yang diterimanya dari radiasi matahari tersebut.
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Judul: Aplikasi pengindraan jauh untuk menduga suhu permukaan dan udara di lahan gambut dan mineral dengan menggunakan metode neraca energi: area study Sampit, Kalimantan tengah
Abstrak: Prediction using satellite imagery can be very useful if used to estimate the temperature in different regions with different land cover types. This study aims to analyze the surface and rising air temperatures in peat lands and minerals, with the energy balance approach using satellite image data TM / ETM +. The methodology undertaken is the determination of the study area, initial processing of satellite images that include: data import images, geometric correction, radiometric, land cover classification, extraction of surface temperature values and calculate the components of energy balance to determine the value of air temperature. The results showed that land use change on mineral and peat soil for 15 years resulted in increased surface temperatures and air temperatures. The range of average surface temperature as a whole in 1989 for mineral soil and peat (27-41) oC, In 2004 the surface temperature of the mineral soil (32-40) oC, and peat (29-35) oC, and the average value range of temperatures (25-36) oC. On the other hand detection of surface temperature using thermal bands of satellite imagery, should pay attention to aspects of the use of their land to avoid misinterpretation, because the condition of land cover types were very influential in the increase or decrease the temperature of the sector in a region.
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Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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