Judul: Percobaan Ekonomi Untuk Mengkaji Pengaruh Signaung dan Sistem Transaksi Dalam Kondisi Informasi Asimetris
Abstrak: Salah satu syarat terbentuknya pasar persaingan sempuma adalah dengan adanya informasi yang sempuma. yang berarti semua penjual dan pembeli mengetahui tentang keadaan pasar. Kenyataan yang sering terjadi di pasar adalah informasi yang tidak seimbang antara penjuaJ dan pembeli (asimetris). Untuk mengatasi hal itu perlu adanya suatu signaling terhadap suatu barang yang akan ditransaksikan.
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Judul: Kajian Pengaruh Signaling Dalam Informasi Asimetris Dengan Percobaan Ekonomi
Abstrak: Pasar dengan infonnasi asimetris pada awalnya akan menguntungkan pihak penjual, namun dalam jangka panjang kemungkinkan akan merugikan pihak penjual itu sendiri, misalnya dalam jumlah transaksi yang semakin menurun. Tujuan penelitian ini untuk mengkaji prilaku penjual dan pembeli dalam pasar informasi asimetris dengan adanya pengaruh signaling. Percobaan dengan signaling dilakukan dengan menggunakan Rancangan Faktorial, dengan 3 faktor yaitu faktor signaling, faktor penjual dan faktor range kualitas, masing-masing terdiri dari 2 taraf, sehingga ada 8 kombinasi perlakuan. Masing-masing perlakuan diamati sebanyak delapan kali. Respons yang diamati dalam percobaan ini adalah contract price, efisiensi pasar, total surplus penjual, total surplus pembeli dan harga penawaran.
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Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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