Judul: Growth Performance of Gourami Fish Osphronemus goramy Supplemented with Insulin Leaf Flour Smallanthus sonchifolius on Feed
Abstrak: Pertumbuhan ikan gurame (Osphronemus goramy) yang lambat merupakan masalah bagi pembudidaya. Suplementasi tepung daun insulin (Smallanthus sonchifolius) berpotensi digunakan untuk meningkatkan kinerja pertumbuhan melalui peningkatan pemanfaatan karbohidrat pakan pada ikan gurame. Penelitian bertujuan memperoleh dosis terbaik penambahan daun insulin terhadap kinerja pertumbuhan ikan gurame. Penelitian menggunakan lima perlakuan yaitu penambahan daun insulin 0,0%, 0,5%, 1,0%, 1,5%, dan 2,0% dengan empat kali ulangan. Ikan dengan bobot 0,97±0,001 g dipelihara di akuarium ukuran 80x40x35 cm3 sebanyak 15 unit dengan padat tebar 15 ekor per akuarium. Pemeliharaan dilakukan selama 60 hari dengan metode pemberian pakan at-satiation. Hasil penelitian menunjukkan pemberian daun insulin 1,5% dapat meningkatkan bobot akhir, laju pertumbuhan spesifik, rasio konversi pakan dan rasio efisiensi protein. Kesimpulan penelitian ini yaitu suplementasi tepung daun insulin 1,5% pada pakan dapat meningkatkan kinerja pertumbuhan ikan gurame., Slow growth of gourami (Osphronemus goramy) is a problem for cultivators. Supplementation of insulin leaf flour (Smallanthus sonchifolius) has the potential to be used to improve growth performance through increased utilization of feed carbohydrates in gourami. The study aimed to obtain the best dose of insulin leaf flour on the growth performance of gourami. The study used five treatments, namely the addition of insulin leaves 0.0%, 0.5%, 1.0%, 1.5%, and 2.0% with four replications. Fish weighing 0.97 ± 0.001 g were reared in aquariums measuring 80x40x35 cm3 as many as 15 units with a stocking density of 15 fish per aquarium. Maintenance was carried out for 60 days with the at- satiation feeding method. The results showed that 1.5% insulin leaf flour supplementation could increase final weight, specific growth rate, feed conversion ratio and protein efficiency ratio. The conclusion of this study is that supplementation of 1.5% insulin leaves in feed can improve the growth performance of gourami.
Keyword: daun insulin, ikan gurame, pertumbuhan, gourami fish, growth, insulin leaf

Judul: Physiology, Biochemistry, and Molecular Responses of Giant Gourami Juvenile Treated by Recombinant Growth Hormone
Abstrak: Ikan gurame (Osphronemus goramy Lac.) merupakan salah satu spesies target revitalisasi perikanan untuk tujuan konsumsi dalam negeri yang produksinya ditargetkan meningkat sekitar 4% per tahun pada tahun 2014. Kendala utama dalam mewujudkan target tersebut adalah laju pertumbuhan somatik ikan gurame yang relatif rendah, yang membutuhkan waktu sekitar 1,5 tahun untuk mencapai ukuran konsumsi (300-500 gram). Target produksi tersebut dapat diwujudkan antara lain melalui penerapan peningkatan kecepatan pertumbuhan ikan gurame menggunakan protein hormon pertumbuhan rekombinan (rGH). Protein rGH telah terbukti mampu memacu pertumbuhan ikan, udang, dan kekerangan, sehingga berdasarkan hal tersebut percobaan ini dilakukan. rGH ikan mas (rCcGH) dan ikan kerapu kertang (rElGH) diekspresikan pada bakteri Escherichia coli strain BL21 (DE3), diekstraksi dan kemudian diujikan ke benih ikan gurame melalui perendaman untuk memacu pertumbuhannya. Jenis rGH yang memberikan efek pertumbuhan terbaik digunakan pada penelitian selanjutnya untuk menganalisis efeknya terhadap proses metabolisme dan ekspresi gen insulin-like growth factors (IGFs) dan growth hormone receptors (GHRs). Pada penelitian ini juga dilakukan kloning gen IGF-1 dan perancangan primer spesifik IGFs dan GHRs ikan gurame untuk analisis kuantitatif polymerase chain reaction (qPCR). Pada tahap akhir penelitian dilakukan uji biokimia untuk mengevaluasi status nutrisi, potensi alergi, dan analisis performa otot daging dan beberapa organ dalam ikan gurame., Aquaculture production of giant gourami in Indonesia is targeted to increase by 4% per year. The main constraint to meet the goal is slow growth of giant gourami, Osphronemus goramy Lac, which takes nearly 1.5 years to grow to a market size of 300-500 g. The target can be achieved by using recombinant growth hormone (rGH) to enhance growth of juvenile. The research was divided into four chapters: (1) analysis of growth and biochemical responses of giant gourami juvenile immersed in recombinant Cyprinus carpio growth gormone with different dosages, (2) analysis of growth, metabolism, and molecular responses of giant gourami juvenile immersed by recombinant Epinephelus lanceolatus (rElGH) and Cyprinus carpio growth hormone (rCcGH), (3) cloning of insulinlike growth factor I (IGF-1) gene and designing specific primer for quatitative PCR (qPCR), (4) evaluation of nutritional properties, allergenic potential, and histological performance of giant gourami immersed with rElGH. The results showed that both rElGH and rCcGH could promote growth gain of juvenile giant gourami. The juveniles immersed by 0.03 mg L-1 rElGH was more efficient and effective to enhance the growth of giant gourami juvenile over 120 mg L-1 rCcGH. The rGH has gluconeogenesis effect, in the muscle and liver rGH stimulates triglyceride uptake by enhancing lipoprotein lipase (LPL) activity and its subsequent storage. The effects of rGH on carbohydrate metabolism may be mediated indirectly via the antagonism of insulin action.
Keyword: Giant gourami, growth hormone, immersion, growth, metabolism, IGF- 1, fatty acid, amino acid, histological performance, histamine

Judul: Analisis dan Implementasi Certificate Authorities (CA) pada Public Switched Telephone Network (PSTN)
Abstrak: Secure Telephone Protocol has ability to authenticate involved party and keep the secrecy of the message sent over the protocol. The secrecy of the message archieved by using symmetric key cryptographic scheme, and authentication archieved using public key cryptographic scheme. One of several party involved in the protocol and hold an important function called Certificate Authorities or CA. CA responsible for generating public key pair for other party involved in the protocol. When CA generates public key pair, several primitive cryptographic scheme used for guarantee the security of the key. Two of them are hash function SHA-1 and El-Gamal public key scheme embedded in Digital Signature Algorithm scheme. The prototype implementing the scheme above developed using Java programming language with JCE addition from Bauncy Castle. The analysis towards implemented prototype indicates that the prototype still has some flaws to be implemented in real world.
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