Judul: Perubahan Mutu Fisik Mentimun (Cucumis sativus L.) Pada Kemasan Plastik Polietilen dan Keranjang Bambu dalam Transportasi Darat
Abstrak: Transport model and selection on packaging type in land transportation of cucumber determine the level of mechanical damage which can degrade the quality of cucumber. Mechanical damage on cucumber grouped into wound scratch, wound rupture, bruising, and wound crack. Mechanical damage will affect the physical quality of cucumber. The objective of this research is to determine effect of some packaging types on cucumber physical quality change (polyethylene plastic and bamboo baskets). The observations were conducted based on the physical quality parameters such as mechanical damage, weight loss, color, hardness, and organoleptic tests. Simulations was performed using a simulator table for 2 hours and 3 hours with an amplitude of 2.5 cm and frequency of 2.75 Hz. Physical qualiy parameter of cucumber were observed before and after simulation. Polyethylene packaging showed better results in cucumber fruit packaging, with less mechanical damage (28.48% and 32.64%), weight loss (10:07% and 10.62%) and general acceptance of cucumber by the panelists after transport with a score of 3.5.
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Judul: Kajian Jenis Kemasan Kaku Terhadap Mutu Buah Mentimun Segar (Cucumis sativus L.) Dalam Simulasi Transportasi Darat
Abstrak: Buah mentimun dapat mengalami kerusakan dalam transportasi karena penanganan yang kasar, penumpukan kemasan yang berlebihan dan kapasitas tiap kemasan lebih dari 30 kg. Hal ini menyebabkan mentimun sering mengalami kerusakan fisik seperti memar, patah, gores dan retak akibat beban yang berlebih serta benturan antar mentimun maupun antara mentimun dengan kemasan. Tujuan dari penelitian ini adalah menganalisis jumlah kerusakan mekanis dan menentukan pengaruh tiga jenis kemasan terhadap mutu buah mentimun setelah simulasi transportasi darat serta menentukan kemasan terbaik untuk pengangkutan mentimun dari ketiga jenis kemasan yang digunakan. Pada penelitian ini kemasan yang digunakan adalah plastik polietilen (kontrol), plastik polietilen + keranjang plastik (PEKP), plastik polietilen + karton gelombang (PEKG), dan lama simulasi adalah 2 jam dan 3 jam. Jumlah kerusakan mekanis paling besar dialami oleh buah mentimun yang dikemas menggunakan plastik polietilen yaitu sebesar 53.96% dan 59.57% untuk lama simulasi 2 jam dan 3 jam. Jenis kemasan dan lama simulasi berpengaruh nyata terhadap susut bobot, kadar air, dan kekerasan. Berdasarkan uji pembobotan dan uji statistik kemasan yang terbaik untuk transportasi mentimun adalah plastik polietilen + karton gelombang (PEKG).
Keyword: jenis kemasan, mentimun, simulasi transportasi darat

Judul: Isolasi Konstruksi dan Rekombinasi Gen Pengkode Reverse Transcriptase asal Simian Retrovirus-2 (SRV-2)
Abstrak: The availability of reverse transcriptase enzym as a valuable component for Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique has been providing by foreign companies. Therefore, it needs more cost and time to provide it. This study is an early step to produce in house reverse transcriptase rekombinant enzym from SRV-2. The purpose of this study was isolated, constructed, and rekombined SRV-2 reverse transcriptase gene to entry clone and destination vector. Isolation was done by PCR amplification technique using three pairs of primers, which are RT I (3282-3683), RTII (3261-3900), and RTIII (3169-4133). Construction and recombination in this study used Gateway method from Invitrogen. SRV-2 reverse transcriptase gene was isolated in this study with A-549 cell inoculated SRV-2 as source of virus. This gene amplify by PCR technique and resulted specific band for RTI 401 bp, RTII 639 bp, and RTIII 964 bp. Analysis of pENTR/SD/TOPO and pDEST17 indicated SRV-2 reverse transcriptase gene code has already inserted to this plasmid. This result indicated SRV-2 reverse transcriptase gene have isolated, constructed, and recombined to entry clone and destination vector.
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