Judul: Pengetahuan dan Sikap Ibu Balita Stunting Usia 24-59 Bulan di Kecamatan Teluk Betung Selatan, Kota Bandar Lampung
Abstrak: Stunting merupakan kondisi anak pendek karena kurang gizi kronik dengan prevalensi tinggi di Indonesia. Perawatan balita stunting dipengaruhi oleh pengetahuan dan sikap ibu mengenai stunting. Penelitian bertujuan menganalisis pengetahuan dan sikap mengenai stunting pada ibu yang memiliki balita stunting usia 24-59 bulan. Penelitian ini menggunakan desain cross-sectional study yang dilaksanakan pada November-Desember 2019 di Kecamatan Teluk Betung Selatan, Kota Bandar Lampung. Subjek berjumlah 60 orang ibu balita yang dipilih dengan metode purposive sampling, dengan kriteria inklusi memiliki anak stunting berdasarkan hasil pengukuran antropometri balita bulan Februari dan Agustus 2019. Data pengetahuan dan sikap mengenai stunting diperoleh dengan menggunakan kuesioner. Data dianalisis secara deskriptif dan diuji menggunakan uji korelasi Spearman dan Chi-square. Hasil penelitian menunjukkan ibu balita memiliki pengetahuan dan sikap positif mengenai stunting yang kurang, yaitu 50.0% dan 61.7%. Sementara itu, semakin tinggi tingkat pendidikan berkorelasi signifikan dengan sikap positif ibu balita mengenai stunting yang semakin baik (r=0.261, p<0.05). Usia ibu, pekerjaan, pendapatan rumah tangga, dan besar keluarga tidak berhubungan signifikan dengan pengetahuan dan sikap ibu mengenai stunting. Selain itu, hasil uji korelasi secara signifikan juga menunjukkan bahwa semakin baik pengetahuan berkorelasi dengan sikap ibu balita mengenai stunting yang semakin baik pula (r=0.544, p<0.05).
Keyword: balita stunting, pengetahuan, sikap

Judul: Pengetahuan, Sikap, dan Praktik Ibu Balita Stunting Usia 24−59 Bulan di Kecamatan Cibungbulang, Kabupaten Bogor
Abstrak: Stunting merupakan salah satu permasalahan gizi kronis di Indonesia yaitu kondisi kurangnya panjang atau tinggi badan balita menurut usianya yang berkaitan dengan pengetahuan, sikap, dan praktik ibu mengenai stunting. Penelitian ini bertujuan untuk mengetahui pengetahuan, sikap, dan praktik ibu mengenai stunting pada balita usia 24−59 bulan. Desain penelitian ini adalah cross sectional study yang dilaksanakan pada bulan November hingga Desember 2019 di Kecamatan Cibungbulang, Kabupaten Bogor. Subjek berjumlah 86 orang ibu yang memiliki balita stunting yang dipilih dengan metode purposive sampling. Hasil penelitian menunjukkan bahwa subjek memiliki pengetahuan dan sikap positif yang kurang mengenai stunting, yaitu 53,5% dan 66,3%. Namun, sebagian besar subjek memiliki praktik yang baik mengenai stunting (91,9%). Terdapat hubungan yang signifikan antara tingkat pendidikan ibu dengan pengetahuan ibu mengenai stunting (p=0,014). Hubungan antara pengetahuan dan sikap ibu mengenai stunting juga menunjukkan hasil yang signifikan berdasarkan uji korelasi Spaerman (p=0,000; r=0,617)., Stunting is one of chronic nutritional problems in Indonesia that is condition of the lack of length or height of toddlers according to their age related to the knowledge, attitude, and practice of mothers regarding stunting. The aim of this study is to determine the knowledge, attitude, and practice of mothers regarding stunting in toddlers aged 24−59 months. This study used a cross sectional study design which was conducted in November until December 2019 in Cibungbulang District, Bogor Regency. The subjects were 86 mothers with stunting toddlers who was selected by purposive sampling method. The results showed that the subjects had poor knowledge and positive attitude regarding stunting, i.e 53.5% and 66.3% respectively. However, most of the subjects had good practice regarding stunting (91.9%). There is a significant relationship between maternal education level and knowledge regarding stunting (p=0.014). The relationship between maternal knowledge and attitude regarding stunting also showed significant results based on the Spearman correlation test (p=0.000; r=0.617).
Keyword: attitude, knowledge, practice, stunting toddler

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.