Judul: Kebiasaan Jajan Anak Sekolah Dasar di SD 036, SD 070 dan SD Muhammadiyah 5 Kota Pekanbaru
Abstrak: The purpose of this study was to examine the habits of eating snacks among the school children at SD 036, SD 070 and SD Muhammadiyah 5 in the town of Pekanbaru. This was a cross sectional study with the subjects of 108 children in Grades 4 and 5(10-12 years). The data on the habit of eating snacks were collected by using a 2x24 hour Food Recall and Food Frequency (FFQ), processed using Nutrisurvey, Microsoft Excel 2007 and analyzed using the Statistical Program for Social Science (SPSS) 16.0 for Windows. The Spearman correlation results indicated there was a significant positive relationship between parent’s (mother and father) education and the number of major foods and beverages, while on the food frequency mother’s education had a significant and positive correlation with the main meal snacks and beverages. The Spearman correlation results also showed a significant negative relationship between mother’s education and the habit of eating snacks (number of snack types and frequency) and between father and mother's education and fruit snack habit (number of snack types and frequency). Further, the Spearman correlation results indicated that there was a significant positive relationship between family income and the habit of eating snacks (the number of minor snack types and snack frequency), and on the fruit snacks there was a significant positive correlation between family income and the number of snack types, while on the beverage snacks there was a significant negative correlation between the amount of snack pocket money and the snack habit (number of snack types and frequency). The amount of snack pocket money and the number of major food snack types were positively and significantly related, and on beverage snacks there was also a positive and significant correlation between the amount of snack pocket money and the snack habit (number of snack types and frequency).
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Judul: Hubungan antara konsumsi jajanan, higiene dan sanitasi dengan morbiditas dan status gizi anak SD Negeri 3 Tangerang Selatan dan SD Negeri Parakan 1 Tangerang Selatan
Abstrak: The objective of this research was to examine the relationship between consumption of snacks, hygiene and sanitation with morbidity and nutritional status of primary school children. The research design was a cross sectional, and subjects were 88 children of 5 th grade students (10-12 years old). The habit of eating snacks was collected through Food Recall 2x24hours. The results showed that most students had a good level of nutrition knowledge category (54.5%), and as many as 78.6% of food vendors had relatively less knowledge about nutrition. A total of 53.4% of students classified as frequent snacks. Most students had a normal nutritional status 67.0% with low morbidity category (44.3%) and moderate (35.2%). Based on Pearson correlation test there was no significant relationship between knowledge of nutrition and food security pocket with frequency (p=0766 ; r=-0032) and the showed that number of snacks (p=0.253 ; r=-0123). Spearman correlation test results there was a significant relationship between knowledge of nutrition and food security and nutritional status (p=0.039 ; r=0.221). There was a significant relationship between energy snacks consumption and nutritional status (p=0.038 ; r=0.614) and morbidity status (p=0.040 ; r=0.714). There was a significant relationship between protein snacks consumption and nutritional status (p=0.041 ; r=0.525) and morbidity status (p=0.036 ; r=0.308).
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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