Judul: Karakterisasi Protein Antigenik Larva 3 Gnathostoma spinigerum pada Ikan Belut Rawa (Monopterus alba) Menggunakan Teknik Immunoblotting
Abstrak: Gnathostoma spinigerum is an intestine nematode in carnivore but can cause human gnathostomiasis. Man is a paratenic host and can acquires the infection by eating non cooked or inadequately processed intermediate hosts, e.g. freshwater fishes, frogs, birds and snakeswhich contain encapsulated third stage larvae. Third stage larvaes (L3s) of G. spinigerum were most found in liver and muscle of swamp eels (Monopterus alba). The objective of this research was to analyze the specific antigens of excretory-secretory (ES) products of G. spinigerum L3s (ES-Ag Gs L3) against rabbit immunoglobulin G (IgG) polyclonal antibody-anti ES-Ag Gs L3s using Western blotting technique. The result of this research was hoped to be a basic for the development of immunodiagnostic and molecular diagnostic of gnathostomias. Excretory-secratory proteins isolation of G. spinigerum L3 were obtained by incubating L3 in RPMI 1640 medium which contains 100 units/ml penicillin G and 100 μg/ml streptomycin for four days at 37°C under 5% CO2 in air. The crude antigen protein of ES-L3-Gs were not purificated. Immunoglobulin G anti ES-Ag Gs L3s were obtained by immunize New Zealand strain white rabbits with ES-Ag Gs L3s. Bands with apparent molecular weights of 12.6, 18.6, 37.9, 41.7 and 49.5 kDa were identified from immunoblotting reactions between IgG-anti ES-Ag Gs L3s and ES-Ag Gs L3s. The proteins of ES from other nematode larvae with apparent molecular weights of 12.6, 18.6, and 37.9 were also recognized by IgG-anti ES-Ag Gs L3s. The specific proteins with molecular weights of 41.7 and 49.5 kDa were potential antigens for immunodiagnostic and molecular diagnostic of gnathostomiasis in swamp eels and human gnathostomiasis in Indonesia.
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Judul: Hubungan Ukuran Tubuh dan Kejadian Kecacingan Gnathostoma spinigerum pada Belut Sawah (Monopterus albus).
Abstrak: Gnathostomiasis is a zoonotic disease that attacks the lungs, the eyes , and the nervous systems, thus causing blindness, paralysis even death. If one consumes swamp eels infected by L3 Gnathostoma spinigerum worm and yet not perfectly cooked, he undoubtedly will suffer from Gnathostomiasis. The purpose of this research is to know the correlation between the body size of an eel and the infection of Gnathostoma spinigerum worm developing in the eel, so that eel-consumers will be cautious about eel-eating and Gnasthostmiasis disease. 124 eel samples were weighed and measured, their visceral organs were taken and cleaned with aquades and analyzed with pressured method . All worm larvas discovered in the eel organs were cleaned Ana kept in ethanol of 70%. Then the worm larvas were indentified morphologically using microscope to observe the existence of Gnathostoma spinigerum worm. All collected data were then analyzed using spearman corelation analysis, and by this, three correlation values were obtained; weight (r = 0.446; p<0.05), length (r =0.427; p<0.05), and diameter (r =0.397; p<0.05). Of the three body size parameters observed, it revealed that they all have positive correlation with gnathostmiasis. This proved that the infection of L3 Gnathostoma spinigerum did not inhibit the growth of the swamp eels (Monopterus albus).
Keyword: Zoonosis., Gnathostomiasis, Correlation, Body Size, Gnathostoma spinigerum, Swamp Eel

Judul: Konsep dan kebijaksaan ruang terbuka bumi serpong damai
Abstrak: Pembangunan yang berlangsung cepat di Jakarta, seringkali menyita ruang-ruang terbuka yang ada. Kebijaksanaan pemerintah dalam mengatur ruang terbuka suatu wilayah, tidak selalu dijalankan sepenuhnya. Kota Baru Bumi Serpong Damai dibangun untuk mengatasi beban kota Jakarta dalam memenuhi kebutuhan penduduknya secara menyeluruh; oleh karena itu, Kota Baru Bumi Serpong Damai disebut sebagai kota mandiri. Keberadaan kota mandiri Bumi Serpong Damai bertujuan untuk mewujudkan suatu kota baru swasembada, serta membentuk suatu kota baru yang memberikan citra kota abad 21. Bentuk skenario kota yang dipilih adalah Skenario Inti Perkotaan. Skenario Inti Perkotaan menempatkan pusat kegiatan berupa Central Bussiness District sebagai penggerak utama. Tata guna lahan Kota Baru Bumi Serpong Damai dibuat dengan memperhatikan kondisi saat ini, struktur kota yang dikehendaki dan memperhatikan kaitan antara potensi setiap bagian tapak dengan fungsi-fungsi yang akan diletakan. Ruang terbuka adalah lahan yang tidak dibangun atau lahan yang di atasnya terdapat bangunan dengan luasan kurang dari 20% dari luasan seluruh lahan. Berdasarkan Instruksi Menteri Dalam Negeri No 14/1988, suatu wilayah seharusnya memiliki ruang terbuka dan ruang terbangun de- ngan perbandingan 40%:60%. Prinsip ruang terbuka Bumi Serpong Damai adalah bahwa ruang terbuka merupakan barang mahal, sehingga harus dimanfaatkan seefisien dan seefektif mungkin. Ruang Terbuka Bumi Serpong Damai ditetapkan dengan mempertimbangkan kebutuhan-kebutuhan masyarakat, kemampuan dalam pembiayaan pembangunan, pengelolaan dan pemeliharaan. Kebijaksanaan Ruang Terbuka Bumi Serpong Damai dengan ruang terbangun adalah perbandingan 40%:60%...
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