Judul: Identification of Entamoeba histolytica in Long-Tailed Macaque (Macaca fascicularis) at Primate Research Center IPB University with Nested PCR
Abstrak: Monyet ekor panjang banyak ditemukan di Indonesia, khususnya di wilayah Bali, Kalimantan, Sumatra, dan Jawa. Interaksi antara manusia dan monyet ekor panjang meningkatkan risiko transmisi penyakit zoonotik akibat infeksi protozoa pada monyet ekor panjang. Entamoeba spp. diketahui sebagai protozoa yang paling banyak menginfeksi monyet ekor panjang. Spesies Entamoeba histolytica memiliki risiko zoonosis. Spesies ini diketahui sebagai penyebab amoebiasis yang menimbulkan ratusan ribu kasus kematian pada manusia. Oleh karena itu, penelitian ini dilakukan untuk mengidentifikasi spesies Entamoeba yang menginfeksi monyet ekor panjang di Pusat Studi Satwa Primata IPB. Metode nested PCR dipilih karena dianggap sebagai metode yang memiliki sensitivitas dan spesifisitas tinggi dalam mengenali spesies Entamoeba. Hasil penelitian menunjukkan bahwa 6 dari 20 (30%) sampel positif Entamoeba spp. dan 5 dari 20 (25%) sampel tersebut positif Entamoeba histolytica. Upaya pencegahan infeksi Entamoeba histolytica pada monyet ekor panjang dapat dilakukan melalui peningkatan sanitasi kandang, pemerhatian manajemen perkandangan, dan pemberian vaksin., Long-tailed macaques are commonly found in Indonesia, especially in Bali, Kalimantan, Sumatra, and Java. The interaction between humans and long-tailed macaques increases the risk of zoonotic disease transmission due to protozoan infection in long-tailed macaque. Entamoeba spp. is known to be the most common protozoan infects long-tailed macaque. Entamoeba histolytica species cause zoonotic risk. This species also known as the cause of amoebiasis which causes hundreds of thousands of deaths in humans. Therefore, this study was conducted to identify species of Entamoeba that infects long-tailed macaques in Primate Research Center IPB University. The nested PCR method was chosen because it is considered to have high sensitivity and specificity in identifying Entamoeba species. The result showed that 6 out of 20 (30%) samples were positive for Entamoeba spp. and 5 out of 20 (25%) of these samples were positive for Entamoeba histolytica. Prevention of Entamoeba histolytica infection in long-tailed macaques can be done through improving cage sanitation, intensifying cage management, and administering vaccines.
Keyword: Entamoeba histolytica, long-tailed macaque, nested PCR, Macaca fascicularis, Primate Research Center IPB University 

Judul: Identifications of Gastrointestinal Parasites in Long-Tailed Monkey (Macaca fascicularis) at The Primate Research Center of IPB  University
Abstrak: Infeksi parasit merupakan salah satu masalah yang sering ditemukan pada satwa primata, termasuk monyet ekor panjang (Macaca fascicularis). Infeksi parasit pada saluran pencernaan perlu diwaspadai karena dapat mengganggu kesehatan monyet ekor panjang di penangkaran dan berpotensi menjadi sumber penyakit zoonotik yang dapat menyebar kepada manusia. Penelitian ini bertujuan mengidentifikasi parasit gastrointestinal pada feses monyet ekor panjang di Pusat Studi Satwa Primata (PSSP) IPB. Sebanyak 69 sampel feses monyet ekor panjang diperiksa menggunakan metode natif dan pewarnaan trichrome untuk mengidentifikasi parasit. Hasil pemeriksaan menunjukkan bahwa terdapat Entamoeba spp., E. polecki, E. coli, E. histolytica/E. dispar, Endolimax nana, Balantidium coli, Iodamoeba bütschlii, Giardia sp., dan Ancylostomatidae, yang diduga bersumber dari air yang digunakan di PSSP IPB. Perubahan konsistensi feses diduga diakibatkan faktor selain infeksi parasit., Parasitic infection is a problem that is often found in nonhuman primates, including the long-tailed monkey (Macaca fascicularis). Parasitic infections in the digestive tract need to be concerned because they can interfere with the well-being of long-tailed monkeys in captivity and have the potential to become a source of zoonotic diseases that can spread to humans. This study aims to identify gastrointestinal parasites in long-tailed monkey feces at the Primate Research Center of IPB University. A total of 69 samples of long-tailed monkey feces were examined using native method and trichrome staining to identify parasites. The results showed that there were Entamoeba spp., E. polecki, E. coli, E. histolytica/E. dispar, Endolimax nana, Balantidium coli, Iodamoeba bütschlii, Giardia sp., and Ancylostomatidae, allegedly derived from the water in PSSP IPB. Change in stool consistency is assumed to be due to factors other than parasitic infection.
Keyword: long-tailed monkey, gastrointestinal parasites, Macaca fascicularis, zoonoses

Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4