Judul: Pengaruh Gaya Kepemimpinan dan Pengembangan Karir Terhadap Kinerja Karyawan PT Jasa Marga (Persero) Tbk Kantor Cabang Jagorawi
Abstrak: Leader is an influential person in a company and in leading an organization, leader have different styles. Career development is one of the employee’s efforts dan plans to achieve future career goals. Career development should be supported by the leader and company in order to create optimal employee performance, so that company goals can be achieved. The purpose of this study were: (1) Analyze employee perceptions regarding leadership styles in PT Jasa Marga (Persero) Tbk Jagorawi Branch Office;(2) analyze the employees' perception of career development at PT Jasa Marga (Persero) Tbk Branch Jagorawi; (3) Analyze employee perceptions of the employee performance in PT Jasa Marga (Persero) Tbk Jagorawi Branch Office;and (4) Analyze the influence of leadership styles and career development on employee performance in PT Jasa Marga (Persero) Tbk JagorawiBranch Office.Type of data used in the study is primary data obtained from open interview and questionnaires, meanwhile the secondary data obtained from books, thesis, information from internet and data-data sourced from PT Jasa Marga (Persero) Tbk JagorawiBranch Office.The analysis tools are descriptive analysis andPartial Least Square (PLS) with the help of software SmartPLS.The samples in this study were employees ofPT Jasa Marga (Persero) Tbk JagorawiBranch Office, by using the technique of probability and nonprobability sampling, namely with the method of proportionate stratified cluster sampling convenience. PLS analysis results stating that leadership styles have a negative impact on employee performance, while career development have a positive influence on employee performance. Model of the influence of leadership styles and career development to give the performance of R-square value of 0.4589.It is interpreted that the construct of performance can be explained by the construct of leadership styles and career development by 45.90% or 54.10% while the rest is explained by variables outside research.
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Judul: Pengaruh Gaya Kepemimpinan Terhadap Kinerja Pegawai Negeri Sipil (PNS) pada Pusat Pelatihan Manajemen dan Kepemimpinan Pertanian (PPMKP) Ciawi Bogor
Abstrak: Civil servants as officers of the state is required to have an excellence, skill, and professional skills to be able to perform government’s tasks and accountability well. To improve performance and create the work motivation, so the companies apply the fair and feasible leaderhip principle. Leadership style becomes the reason of the research implementation because of problems that occurred starting from the turn of the leader. This research aims to determine the leadership style applied by the leader to the civil servants by using descriptive analysis, determine the performance of civil servants by using descriptive analysis. Analyze the influence of leadership style to the performance of civil servants by using multiple linear regeresi analysis. This results show the respondents agree with the four styles of leadership that is authoritarian, participative, delegative, and situational. Employee fulfills the performance standards set out in the list of employee job execution (DP3). The Leadership style is not a positive influence to the employee performance.
Keyword: linear regeresi analysis, performance of civil servants, leadership style

Judul: Influenza Specific Immunoglobulin Y Purification from Egg Yolk with Sodium Chloride Precipitation Method
Abstrak: Imunoglobulin Yolk (IgY) yang terdapat dalam kuning telur telah banyak dimanfaatkan untuk imunoterapi dan imunodiagnostik. Salah satu kendala dalam pemurnian IgY dari kuning telur adalah pemisahan Ig dengan lemak, sehingga teknik pemurnian Ig selalu berkembang. Penelitian ini bertujuan untuk melakukan pemurnian IgY dari kuning telur dengan metode pengendapan menggunakan sodium chloride (NaCl) serta melakukan karakterisasi IgY spesifik influenza. Sodium chloride dipilih karena bahan kimia ini relatif murah, ramah lingkungan, dan tidak merusak imunoaktivitas IgY. Kuning telur yang digunakan dalam penelitian ini berasal dari telur ayam layer yang divaksinasi dengan vaksin influenza Flubio® yang berisi virus Influenza A/H3N2, A/H1N1 dan B/Washington. Pemurnian IgY hanya dilakukan pada kuning telur yang mengandung antibodi spesifik influenza. Deteksi keberadaan antibodi spesifik influenza ditentukan dengan uji agar gel presipitasi (AGP) dan metode enzyme linked immunosorbent assay (ELISA). Konsentrasi IgY hasil pemurnian dihitung dengan spektrofotometer dan karakterisasi protein dilakukan dengan metode Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Konsentrasi protein dari hasil pemurnian IgY memiliki nilai rata – rata 50,403 mg/mL. Hasil SDS-PAGE menunjukkan enam pita protein, yaitu struktur IgY utuh (174,63 kDa), Apoprotein-LDL (129,77 kDa), rantai berat IgY (66,54 kDa), Fab ( 47,65 kDa), rantai ringan IgY (21,85 kDa), dan β-livetin (36,74 kDa). IgY hasil pemurnian masih dapat berikatan dengan antigen influenza berdasarkan uji ELISA. Hasil penelitian ini menunjukkan bahwa pemurnian IgY menggunakan metode pengendapan NaCl dapat memurnikan IgY dengan struktur utuh, meskipun masih ada dua protein tidak spesifik hasil pemurnian. IgY yang dapat berikatan dengan antigen influenza mengindikasikan prospek pemanfaatannya untuk pencegahan influenza dan imunodiagnostik., Yolk immunoglobulin (IgY) contained in egg yolk has been widely used for immunotherapy and immunodiagnostics. One of the obstacles in IgY purification from egg yolk is separating Ig from fat, so that IgY purification techniques are always developing. This study aimed to purify IgY from egg yolk using sodium chloride (NaCl) precipitation method and to characterize influenza-specific IgY. Sodium chloride was chosen because this chemical is relatively unexpensive, environmentally friendly, and does not impair IgY immunoactivity. The egg yolks used in this study were collected from layer eggs vaccinated with the Flubio® influenza vaccine, which contains the Influenza A/H3N2, A/H1N1 and B/Washington viruses. IgY purification was only carried out on egg yolks containing influenza specific antibodies. The presence of influenza-specific antibodies was determined by the agar gel precipitation (AGP) test and the enzyme linked immunosorbent assay (ELISA) method. The purified IgY concentration was determined using spectrophotometer, and protein characterization was performed using the Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) method. Protein concentration from purification has a mean value of 50,403 mg/mL . SDS-PAGE result showed six protein bands, namely intact IgY structure (174,63 kDa), Apoprotein-LDL (129,77 kDa), IgY heavy chain (66,54 kDa), Fab (47,65 kDa), IgY light chain (21,85 kDa), and β-livetin (36,74 kDa). The purified IgY still able to bind with influenza antigen based on the ELISA test. The results of this study indicate that purification of IgY using the NaCl precipitation method can purify IgY with intact structure, although there are still two unspecific proteins in the purification results. The resulting IgY can bind to influenza antigens indicating the
Keyword: Immunoglobulin Y, influenza, egg yolk, purification, sodium chloride