Judul: IPB (Bogor Agricultural University)
Abstrak: Tujuan penelitian ini adalah untuk (1) mengetahui biotipe H. abortus isolat lapang yang menginfeksi sapi di beberapa daerah di Indonesia, (2) mengetahui beberapa sifat patogenitasnya pada hewan percobaan marmot dan (3) mengetahui respon marmot terhadap vaksin Brucella yang kemudian ditantang dengan 8- abortus. isolat lapang. Hipotesis yang akan diuji dalam penelitian ini adalah (1) biotipe B- abortus isolat lapang terdiri dari beberapa macam biotipe dan yang dominan adalah biotipe B, (2) berdasarkan tingkat virulensinya kebanyakan isolat lapang tergolong galur virulen dan (3) respon marmot terhadap vaksin Brucella yang kemudin ditantang dengan R. abortus isolat lapang lebih baik terhadap vaksin aktif dari pada vaksin inaktif.
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Judul: Pengembangan kandidat vaksin iradiasi Brucella abortus isolat lapang
Abstrak: Brucellosis is a strategic contagious animal disease in Indonesia, which is caused by Bru/:ella abortus (B. abortus). Brucellosis causes abortion, still birth, orchitis; arthritis, infertility and sterility lowering milk production. Eradication of cattle in many countries have been conducted by test and slaughter and vaccination programme. At the moment there are two vaccinations available to control bovine brucellosis; those are B. abortus S 19 and B. abortus RB51. One of the problems in the eradication is not enough a vaccine. The objective of this research was to develop irradiated brucellosis vaccine from B. abortlls local isolate through isolation, identification and biotyping B. abortus from field cases. Determination of B. abortus radiation dose and in vivo study was done in guinea pigs. The samples were collected from milk (Bandung, west Jawa), amnion fluids (diagnostic sample) and higroma fluids (Be1u, NIT). In this study we use three isolates that were SBDG-13C (milk), CAM-08/306 (amnion fluids), and CH09- BL (higroma fluids). Biotyping of the three isolates categorized as B. abortus biotype I. Standard curves of B. abortus was made by calculating bacteria cells number indirectly. Linear equation of the standard curve of SBDG- J3C; CAM- 08/306 and CH09-BL isolates were y = 7.2119x + 6.7723; y = 3.889lx + 7.5278 and y = 2.9829x + 7.9612 respectively (x is absorbance value and y is log number of cells/ml). Observation on the growth of the isolates after Gamma irradiation showed variation responses to the value LDso of isolate CAM-08/306, CH09-BL and SBDG-I3C were 13.5; 11.9, and 17.9 Gy, respectively. The in vivo study showed that the spleen of irradiation vaccinated guinea pigs was a swelling (splenomegaly) and contained 102 to 106 CFU bacteria. Based on the results, It seems likely that those three isolates have not been defined as candidate isolate for brucellosis vaccine.
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Judul: Hybrid solar cells of ZnO nanoparticles/chlorophyll was modified metal ions (Zn2+ and Cu2+ )
Abstrak: Zinc Oxide (ZnO) is a n-type semiconductor with a wide the energy band (Band Gap) is 3.37 eV at room temperature and 3.34 eV at low temperature with a large exiton binding energy is 60 meV. ZnO has been synthesized by hydrothermal method, resulting in a decrease in particle size over the duration of the hydrothermal changes. Crystallographic structure of ZnO were characterized using XRD, has shown the characteristic pattern of a hexagonal wurtzite structure, the crystallite size increases to changes in particle size. Changes in particle size has also led to a change in the absorption peak of ZnO as has been demonstrated posted under test using the optical properties of UV-Vis spectrophotometer. Changes of this uptake has also led to changes in the energy band gap, which increases its band gap energy of the particle size. In electronic applications, especially in solar cells. Chlorophyll is considered less effective, because of their very nature are easily degraded as a result of several reactions which are acid, lighting and temperature. The results of the stability test using a halogen lamp 34 W/m2 for 5 hours, that the substitution of chlorophyll with metal ions Zn2+ and Cu2+ has been shown to form more stable than natural chlorophyll. The characterization of the optical properties, the combination of ZnO / chlorophyll in the form of hybrid films has led to a shift in the absorption region of ZnO becomes more widely from the UV to Visible. ZnO/chlorophyll has absorption range of 350-515 nm, ZnO / Zn-feofitin 332-401 nm and 332-632 nm for Cu-feofitin. The combination of ZnO/chlorophyll and CuSCN as a solid electrolite in hybrid solar cell, a modified chlorophyll with metal ions Zn2+ and Cu2+ has been shown to form a more stable I-V curve. It is characterized by its fill factor value greater than natural chlorophyll. Nevertheless, solar cells with natural chlorophyll has been able to produce higher efficiency values of chlorophyll were substituted.
Keyword: Hydrothermal, ZnO/Chlorophyll modified, Hybrid Solar Cell