Judul: Genetic Diversity Analysis of Oil Palm (Elaeis guineensis Jacq) Based on RAPD (Random Amplified Polymorphic DNA) Using Primer OPJ.
Abstrak: Random Amplified Polymorphic DNA (RAPD) dapat menjadi salah satu alternatif untuk menyeleksi tanaman kelapa sawit berproduktivitas tinggi dan tahan terhadap Ganoderma sp. Pada penelitian ini RAPD digunakan untuk mempelajari keragaman genetik 58 pohon kelapa sawit. Tahapan penelitian dimulai dari isolasi DNA, amplifikasi menggunakan primer OPJ, dan analisis filogenetik menggunakan metode UPGMA (Unweighted Pair Group Method Arithmatic Mean) dengan software NTSYS. DNA hasil isolasi memiliki kualitas yang baik berdasarkan analisis spektofotometri dan elektroforesis gel. Amplifikasi DNA dengan 20 primer seri OPJ menghasilkan 1.592 pita DNA dari 115 lokus, sebesar 68% diantaranya merupakan pita polimorfik. Analisis kemiripan genetik menunjukkan nilai koefisien kemiripan genetik sebesar 49% - 95%. Hasil pengelompokkan populasi kelapa sawit pada nilai kemiripan genetikRandom Amplified Polymorphic DNA (RAPD) dapat menjadi salah satu alternatif untuk menyeleksi tanaman kelapa sawit berproduktivitas tinggi dan tahan terhadap Ganoderma sp. Pada penelitian ini RAPD digunakan untuk mempelajari keragaman genetik 58 pohon kelapa sawit. Tahapan penelitian dimulai dari isolasi DNA, amplifikasi menggunakan primer OPJ, dan analisis filogenetik menggunakan metode UPGMA (Unweighted Pair Group Method Arithmatic Mean) dengan software NTSYS. DNA hasil isolasi memiliki kualitas yang baik berdasarkan analisis spektofotometri dan elektroforesis gel. Amplifikasi DNA dengan 20 primer seri OPJ menghasilkan 1.592 pita DNA dari 115 lokus, sebesar 68% diantaranya merupakan pita polimorfik. Analisis kemiripan genetik menunjukkan nilai koefisien kemiripan genetik sebesar 49% - 95%. Hasil pengelompokkan populasi kelapa sawit pada nilai kemiripan genetik
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Judul: Analisis Keragaman Genetik Kelapa Sawit (Elaeis guineensis Jacq) Asal Jawa Barat Dengan Penanda RAPD (Random Amplified Polymorphic DNA)
Abstrak: Genetic diversity study is the way to clustering an individual in a population for various prospect such as plant improvement. Oil palm (Elaeis guineensis Jacq) in this research are a population of oil palm that still exist since Holland colony and estimate have resistances to Ganoderma sp. Genotype information from those were conducted by RAPD. This research aims to clustering 58 oil palm trees by RAPD method. The research consist of four stages that begin from DNA isolation, DNA amplification, genetic similarity analysis, and construction of phylogenetic tree by UPGMA (Unweighted Pair Group Method Arithmatic Mean) method in NTSYS. The result of DNA isolation were showed good band and 12000 bp in size. The DNA amplification with 20 random primer were resulted 1870 DNA band from 229 locus. About 95% among of them are polymorphic band. Similarity analysis was showed the genetic similarity coeficient value about 69% - 95%. The clustering at genetic similarity coeficient value 76% was divided the population into two big group. The increasing of genetic similarity coeficient value will be obtained more cluster. This genotype information can be used as references in oil palm improvement program
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Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4