Judul: Analisis nilai anak, investasi anak, dan potensi perdagangan anak (kasus di Kabupaten Subang)
Abstrak: Jumlah korban perdagangan anak dari tahun ke tahun semakin meningkat. Penelitian ini bertujuan menganalisis nilai anak, investasi anak, dan potensi perdagangan anak pada keluarga yang memiliki anak remaja dengan melibatkan 30 keluarga. Contoh dipilih secara convinience dengan kriteria anak remaja yang menempuh Sekolah Menengah Pertama (SMP) dari keluarga lengkap dan bertempat tinggal di Kecamatan Pagaden. Pengumpulan data dilakukan melalui wawancara dengan kuisioner. Analisis data yang digunakan adalah analisis deskriptif dan inferensia. Persepsi nilai anak, investasi anak, dan potensi perdagangan anak berada pada kategori sedang. Pendapatan keluarga berhubungan negatif nyata dengan nilai ekonomi anak. Pendidikan ayah, pendidkan ibu, dan pendapatan keluarga berhubungan positif signifikan dengan investasi anak. Pendidikan ayah dan ibu memilki hubungan negatif signifikan dengan potensi perdagangan anak. Nilai anak dan investasi anak tidak memiliki hubungan dengan potensi perdagangan anak.
Keyword: Value of child, Investment of child, Potential of human trafficking

Judul: Nilai-nilai Keluarga, Interaksi Keluarga dan Potensi Perdagangan Manusia (Kasus di Kabupaten Cianjur)
Abstrak: The aim of this research was to examine the relationship between family interaction with potential human trafficking. The research was conducted in the Sukatani village, Haurwangi District, Cianjur Regency. This is umbrella research with uses nonprobability sampling technique with purposive as many 60 people. Samples of this research was the wife of a poor family. The data collected by interview based on questionnaire. The results showed that there was negative significance relationship between parent child interaction with potential trade man.
Keyword: commitment, family interaction, family values, potential for human trafficking, poverty

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.