Judul: In vitro regeneration of terubuk (Saccharum edule)
Abstrak: Terubuk is one of the potential vegetable known as cauliflower sugarcane. It is characterized by unusual, swollen and aborted inflorescences, which is sweet and edible. The inflorescences are used as a source of food in Fiji, New Guinea, Indonesia and Malaysia by the indigenous people. The inflorescences are abnormal in the sense that they remain enclosed within the leaf-sheaths, forming a compact mass about the size of a banana fruit. Terubuk is produced and consumed locally and traded in local market only. In Indonesian’s traditional market, it is sold in bunches of 10. Terubuk is closely related to Saccharum officinarum, S. spontaneum, and S. robustum. It is exclusively propagated by cuttings or by division of clumps. To provide more inflorescences, it needs higher plants production. Its production needs cutting materials or propagules. Conventional propagation requires a lot of planting material. Tissue culture is an alternative propagation technique to solve the inavailability of plant material. The objective of this research was to obtain the best method to propagate terubuk using in vitro micropropagation through direct and indirect organogenesis. Flower stalks were used as explants. The explant’s sterilization was done by spraying the inflorescences with alcohol 96 %, then the flower was burnt. The indirect organogenesis using calli induction showed that the best medium was MS + 3.0 mg l-1 2,4-D + 1.0 mg l-1 kinetin. This media did not produce shoots from calli proliferation stage. It was only able to produce roots. The other method was by direct organogenesis. It was shown that the best medium was MS + 0.25 mg l-1 thidiazuron + 0.1 mg l-1 NAA + 0.25 mg l-1 GA3 to produce shoots in 2 weeks without putting the explants in calli induction medium. Shoots obtained through the media varied in size. Only shoot with 1-3 cm in size were rooted. Root formation required full strength of MS salt. The percentage of success in rooting ranged from 50-80%. Acclimatization has been done, however, after 2 weeks the planlet did not survive.
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Judul: Induksi Kalus Vanili (Vanilla planifolia Andrews) var. Sovania Agribun secara In Vitro
Abstrak: Vanili merupakan tanaman yang memiliki nilai ekonomis tinggi. Penyakit busuk batang vanili (BBV) yang disebabkan oleh Fusarium oxysporum f.sp. vanillae kerap menjadi masalah utama dalam pembudidayaan vanili. Pengadaan varietas unggul seperti Vanilla planifolia var. Sovania Agribun menjadi salah satu upaya dalam pengendalian BBV. Untuk mendukung ketersediaan bahan tanamnya, stek batang dapat dilakukan, namun cara tersebut membutuhkan waktu lama dan bahan induk yang banyak. Kultur jaringan dapat menjadi metode perbanyakan tanaman yang dapat dipilih. Penelitian ini bertujuan mendapatkan metode induksi kalus organogenik yang sesuai untuk pertumbuhan eksplan vanili menggunakan kombinasi zat pengatur tumbuh auksin dan sitokinin. Perlakuan kombinasi 2,4-D dan BAP berbagai konsentrasi, dengan eksplan pucuk apikal, buku ke-1, dan buku ke-2 digunakan dalam penelitian ini. Penelitian disusun menggunakan rancangan acak lengkap (RAL) dengan 9 ulangan untuk setiap perlakuan, pada masing-masing sumber eksplan. Kalus mulai tumbuh pada minggu ketiga hingga ketujuh setelah kultur. Persentase pembentukan kalus sebesar 66,67% dihasilkan dari buku ke-1 yang dikulturkan dalam media MS dengan perlakuan 1,0 mg/L 2,4-D + 0,5 mg/L BAP. Kalus yang terbentuk bertekstur remah dengan warna putih hingga putih-kekuningan, serta tekstur kompak dengan warna kuning. Kata kunci: 2,4-D, BAP, perbanyakan tanaman, toleran busuk batang., Vanilla is a plant that has high economic value. Vanilla stem rot disease (SRD) due to Fusarium oxysporum f.sp. vanillae is a major constraint to vanilla production in Indonesia. Procurement of superior varieties such as Vanilla planifolia var. Sovania Agribun is one of the efforts to control SRD. To provide a sufficient number of planting materials, stem cutting can be done but it requires extensive time and mother plant sources. In vitro culture techniques is an alternative for plant propagation. This study aimed to obtain a suitable method for an optimal organogenic callus induction using combinations of auxin and cytokinin. Treatment combinations of 2,4-D and BAP at various concentrations were applied on apical shoot, the first node, and the second node explants. The experiment was arranged using a completely randomized design (CRD) with 9 replications for each treatment for each explant source. Callus began to grow in the third to seventh week after culture. The first node explants showed better callus initiation, with 66,67% of explants forming callus when cultured on MS with 1,0 mg/L 2,4-D + 0,5 mg/L BAP. The callus had friable, white to yellowish-white in color, and yellow compact textures. Keywords: 2,4-D, BAP, plant propagation, tolerant to stem rot.
Keyword: 2,4-D, BAP, perbanyakan tanaman, toleran busuk batang

Judul: Approximation of the Median of Poisson Distribution Using the Relation of Poisson-Gamma Distribution
Abstrak: A major drawback of median is that median cannot usually be defined in closed form, even in the cases where their distribution functions are known. This study gives an approximation of the closed form for the median of the Poisson distribution by using the relation of Poisson-Gamma distribution. The approximation uses elementary techniques based on the monotonicity of certain sequences involving tail probabilities of the Poisson distribution and the Central Limit Theorem. Furthermore, a closed form expression for the mean absolute deviation 􀜧|􀜰􀰒 􀵆 􀜯􀝁􀝀􁈺􀜰􀰒􁈻| is also studied. The median is relevant because of the fact that it minimizes the mean absolute deviation of a Poisson random variable (􀜰􀰒􁈻 by considering median as central tendency.
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