Judul: Analisis Keragaman Genetika Tanaman Kunyit dan Temulawak secara Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) Menggunakan Primer OPA-OPD 6-10
Abstrak: There are many studies on turmeric (Curcuma domestica Val.) and wild ginger (Curcuma xanthorrizha Roxb.) from Indonesia is only about the content of secondary metabolites, but research on the genetic diversity information of turmeric and wild ginger plants has never been done before. This study was aimed to analyze the fingerprint pattern of Indonesian native turmeric and wild ginger plants by using OPA-OPD 6-10 primer series to obtain a phylogenetic tree of these plants. The steps of this research were DNA isolation, amplification of DNA, then analysis and construction of phylogenetic trees using the UPGMA (unweighted pair group method arithmetic mean) with NTSYS software. The resulted DNA showed good bands and had a size between 11000-12000 bp with the range of purity between 1091-2625. DNA amplification with 20 random OPA-OPD 6-10 primers produced 259 bands (100% polymorphic). Analysis of genetic similarity indicated a genetic similarity coefficient 0.3033-0.6721 for turmeric plant and 0.1939-0.9082 for wild ginger plant. This value indicated that the turmeric and wild ginger plants from Indonesia has a high level of genetic diversity.
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Judul: Analisis Polimorfisme DNA Tanaman Kunyit (Curcuma longa L.) dan Temulawak (Curcuma xanthorrhiza Roxb) Lokal Menggunakan Marka RAPD
Abstrak: Tanaman kunyit (Curcuma longa L.) dan temulawak (Curcuma xanthorrhiza Roxb) lokal Indonesia yang tersebar dan mudah ditemukan di wilayah Indonesia banyak digunakan sebagai obat tradisional. Kandungan kurkumin pada kedua tanaman tersebut memiliki banyak khasiat kesehatan, yaitu sebagai obat antiproliferatif dan antiinflamasi. Adanya polimorfisme DNA pada kedua tanaman tersebut di beberapa lokasi Pulau Jawa belum diidentifikasi. Oleh karena itu, perlu dilakukan analisis genetik untuk menentukan keragaman kedua tanaman tersebut. Salah satu teknik yang biasa digunakan untuk analisis keragaman genetik adalah PCR RAPD. Tujuan penelitian ini mengisolasi DNA tanaman kunyit dan temulawak dan menentukan pola genetik tanaman tersebut dengan PCR RAPD. PCR dilakukan dengan menggunakan 20 primer acak OPA, OPB, OPC, dan OPD (1-5). Ekstraksi DNA menggunakan metode modifikasi Zheng et al. 1995 pada 13 sampel daun temulawak dan kunyit. Hasil menunjukkan bahwa primer yang dapat mengamplifikasi DNA sampel, yaitu: primer OPA-1, OPA-3, OPA-4, OPC-1, OPC-2, OPC-4, dan OPD-4. Selain itu, hanya tiga sampel yang dapat teramplifikasi, yaitu: temulawak Wonogiri, temulawak Karang Anyar, dan kunyit Wonogiri. Sampel yang teramplifikasi memiliki pola genetik yang berbeda yang dihasilkan oleh setiap primer acak sehingga kedua puluh primer acak dapat digunakan sebagai marka untuk analisis fingerprint.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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