Judul: Enzyme Activitties of Black Soldier (Hermeria illucens) Larvae
Abstrak: The disproportion of food demand and food supply caused by population growth, limited land area, and resources prompt the search for novel and sustainable food alternatives. Scientific research has shown that edible insects are an ideal fit, providing many advantages. Typical edible insects consumed, like sago larvae in Eastern Indonesia and mealworms in Southeast Asia, are believed to emerge as functional food products with the combination of their high nutritional value and bioactive compounds findings. An insect with many positive properties, the black soldier fly (Hermetia illucens), whose larvae have been reported as one of the promising food sources. Though there have been studies on the antimicrobial activities and antithrombotic activity of Black soldier fly larvae (BSFL), no report is being made on its fibrinogenolytic activity. In the present study, various enzymatic including the fibrinogenolytic activities were identified. Results showed that the BSFL extract possessed proteolytic, amylolytic, chitinolytic, and cellulolytic activities. Excreted proteolytic and chitinolytic activities were also observed from BSFL whole body. Moreover, the BSFL crude extract was confirmed to have fibrinogenolytic activity performed by zymography and SDS PAGE analysis. Considering the results, the BSFL might suggest as sources for functional food alternative and bioactive substances productions.
Keyword: Enzymatic activities, Fibrinogenolytic activity, Future food alternatives, Hermetia illlucens larvae

Judul: Effect of Black Soldier Fly (Hermetia illucens) in the Ration on Performance, Egg Quality and Immune Response in Laying Hens
Abstrak: Industri perunggasan masih menghadapi berbagai kendala, antara lain masalah bahan baku pakan yang masih bergantung pada impor dan masalah pelarangan antibiotik pemacu pertumbuhan. Oleh karena itu, diperlukan upaya pemanfaatan sumber protein alternatif yang juga memiliki aktivitas antibakteri dan imunomodulator. Larva Black Soldier Fly (Hermetia illucens) kaya akan AMP (antimicrobial peptide) dan memiliki kandungan asam laurat yang tinggi 49.18% dapat berfungsi sebagai antibakteri. Penelitian ini bertujuan mengevaluasi pengaruh subtitusi tepung ikan dengan tepung larva BSF terhadap performa produksi, kualitas telur, serta status kesehatan dan meningkatkan respon kekebalan ayam petelur serta melihat aktivitas antibakteri ekstrak tepung larva BSF secara in vitro. Uji aktivitas antibakteri dilakukan terhadap bakteri Escherichia coli dan Salmonella enteritidis dengan enam level pengenceran (10 mg ml-1, 20 mg ml-1, 40 mg ml-1, 80 mg ml-1, 160 mg ml-1 dan 320 mg ml-1). Feeding trial menggunakan empat perlakuan pakan yaitu P0 (tepung ikan 8%), P1 (tepung ikan 4% + BSF hidup 6.03%), P2 (tepung ikan 4% + BSF kering 5.85%) dan P3 (tepung ikan 4% + BSF ekstrak 5.63%). Desain percobaan yang digunakan adalah rancangan acak lengkap (RAL) dengan empat perlakuan, lima ulangan dan setiap ulangan terdiri atas 10 ekor ayam petelur, sedangkan untuk aktivitas antibakteri menggunakan 6 level pengenceran dan 3 ulangan. Hasil penelitian menunjukkan bahwa BSF ekstrak memiliki aktivitas antibakteri mulai dari level 320 mg/mL dan semakin meningkat seiring dengan meningkatnya dosis (P<0.05). Penggunaan 5.63% larva BSF ekstrak metanol sebagai sumber protein pengganti 50% protein tepung ikan dalam ransum ayam petelur dapat memberikan performa dan kualitas telur yang baik tanpa mempengaruhi status kesehatan ayam petelur. Penggunaan larva BSF hidup dalam ransum dapat mengurangi biaya konsumsi dan pengolahan pakan sehingga keuntungan dapat maksimal. Larva BSF ekstrak metanol terbukti memiliki kemampuan antibakteri sehingga dapat meningkatkan respon kekebalan ayam petelur secara optimal., The poultry industry still faces various obstacles, including the problem of feed raw materials which are still dependent on imports as well as problem of prohibiting growth-promoting antibiotics. Therefore, efforts are needed to utilize alternative protein sources that also have antibacterial and immunomodulatory activities. Black Soldier Fly (Hermetia illucens) larvae has an antimicrobial peptide (AMP) and has a high content of lauric acid is 49.18% which functions as an antibacterial. This study aims to evaluate the effect of fish meal substitution with BSF larvae flour on production performance, egg quality, health status, increasing immunity of laying hens as well as looking at the antibacterial activity of BSF larvae extract in vitro. Antibacterial activity test was carried out on Escherichia coli and Salmonella enteritidis with six levels of dilution (10 mg ml-1 , 20 mg ml-1 , 40 mg ml-1 , 80 mg ml-1 , 160 mg ml-1 and 320 mg ml -1 ). The feeding trial used three feed treatments namely P0 (8% fish meal), P1 (5% fish meal + 6.03% fresh BSF), P2 (5% fish meal + 5.85% dried BSF) and P3 (5% fish meal + 5.63% BSF extract). The experimental design used was a completely randomized design (CRD) with four treatments, five replications and each repetition consisted of 10 laying hens, while for antibacterial activity using 6 levels of
Keyword: Hermetia illucens, laying hens, production performances, egg quality, health status

Judul: Karakteristik Tripel Pithagoras Dari Sudut Pandang Hiperbolik
Abstrak: Misalkan (a, b, c) merupakan tripel pithagoras (TP), jika dipilih x = cia dan y = bla maka diperoleh titik (x, y) pada cabang kanan hiperbola satuan x2+y2 = 1. Dengan demikian sembarang TP dipandang berperan dalam menentukan titik-titik rasional dari Hiperbola satuan yang disebut sudut pandang hiperbolik.
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