Judul: Natural regeneration and distribution patterns of pasang (Quercus sundaica Blume) at various heights on Mount Slamet, Baturraden, Central Java
Abstrak: Pasang (Quercus sundaica) merupakan salah satu tanaman dari famili Fagaceae. Penelitian dilakukan untuk mengetahui struktur dan komposisi pohon, pola sebaran, permudaan jenis Q. sundaica, serta hubungan sifat fisik lingkungan dengan jenis pohon Q. sundaica. Pengambilan data dilakukan pada dua jalur yaitu Jalur KRB (Kebun Raya Baturraden) dan Jalur P7 (Pancuran 7) serta pada dua zona yaitu sub-montana dan montana. Analisis data yang digunakan yaitu analisis vegetasi, analisis tanah, serta model linear regresi berganda prosedur stepwise. Hasil penelitian menunjukkan bahwa ditemukan 59 jenis tumbuhan, 29 famili, dan 48 marga. Nilai INP tertinggi jenis Q. sundaica terdapat pada Jalur KRB zona montana dan jalur tersebut memiliki kerapatan yang lebih tinggi dibandingkan dengan Jalur P7. Faktor yang berpengaruh terhadap keberadaan Q. sundaica yaitu kelembaban, pH, kapasitas tukar kation (KTK), P-potesial dan K-potensial. Permudaan alami jenis Q. sundaica memiliki nilai 0 yang menandakan pada tingkatan semai, pancang, dan tiang tidak ditemukan jenis tersebut kecuali pada tingkat pancang di zona montana Jalur P7., Pasang (Q. sundaica) is a plant from the Fagaceae family. The study was conducted to determine the structure and composition of trees, distribution patterns, regeneration of the Q. sundaica species, and the relationship between the physical properties of the environment and the Q. sundaica tree species. Data collection was carried out on two lines, namely KRB Line (Baturraden Botanical Gardens) and P7 Line (Pancuran 7) also in two zones, namely sub-montane and montana. Analysis of the data was used vegetation analysis, soil analysis, and multiple regression linear model stepwise procedure. The results showed that there were 59 species, 29 families, and 48 genera. The highest INP value for the species Q. sundaica was found in KRB Line on montana zone and that line also had a higher density than the P7 line. Factors that affect the presence of Q. sundaica were humidity, pH, cation exchange capacity (CEC), P-potential, and K-potential. Natural regeneration of the species Q. sundaica had a value of 0 which indicates that at the seedling, sapling, and pole levels, this species was not found except at the sapling level of the montana zone on Line P7.
Keyword: distribution zone, growing place, Q. sundaica, spread

Judul: Distribution Pattern, Habitat Characteristics, and Regeneration of Krangean (Litsea cubeba) at Mount Slamet, RPH Baturraden, KPH Banyumas Timur
Abstrak: Krangean (Litsea cubeba) adalah salah satu jenis yang mendiami wilayah Gunung Slamet. Bagian pohon krangean memiliki aroma sitrus sehingga dapat dimanfaatkan sebagai penghasil minyak atsiri yang potensial. Kondisi permudaan pohonnya di alam sulit ditemukan sehingga diperlukan informasi mengenai preferensi ekologi yang tepat guna tujuan budidaya dan konservasi. Tujuan penelitian ini adalah mengidentifikasi komposisi dan struktur tegakan, pola sebaran, karakteristik habitat, dan regenerasi krangean (L. cubeba) di hutan lindung Gunung Slamet. Penelitian ini menggunakan metode analisis vegetasi dengan membuat plot berukuran 30 m × 30 m sebanyak 18 plot yang dibagi menjadi Jalur Baturraden dan Pancuran 7. Hasil penelitian menunjukkan pola persebaran krangean cenderung mengelompok (clump) dan kondisi regenerasi yang buruk karena hanya ditemukan pada tingkat tiang dan pohon. Kondisi habitat L. cubeba di Gunung Slamet berada pada ketinggian 1018-2032 mdpl dengan kelerengan curam hingga sangat curam (30 - 59,10%), suhu mulai 11℃ hingga 22,5℃, dan kelembaban 74 - 92%. Jenis tanah yang teramati adalah jenis tanah latosol dengan kategori pH agak masam. Faktor lingkungan yang diujikan tidak berpengaruh nyata pada luas bidang dasar (LBDS) krangean dengan R2 sebesar 55,37%., Krangean (Litsea cubeba) is one of the species that inhabits the area of Mount Slamet. All parts of the krangean tree have a citrus scent so that it can be used as a potential essential oil producer. The condition of tree regeneration in nature is difficult to find, so information on appropriate ecological preferences for cultivation and conservation purposes is needed. The purpose of this study was to identify the composition and structure of stands, distribution patterns, habitat characteristics, and regeneration of krangean (L. cubeba) in the protected forest of Mount Slamet. This study used a vegetation analysis method by making plots measuring 30 m×30 m as many as 18 plots which are divided into Baturraden and Pancuran 7 route. tree. The habitat conditions of L. cubeba on Mount Slamet is at an altitude of 1018-2032 masl with steep to very steep slopes (30-59,10%), temperatures ranging from 11℃ to 22,5℃, and humidity of 74 - 92%. The type of soil observed was latosol soil with a slightly acidic pH category. The environmental factors tested did not significantly affect the basal area (BA) of the krangean with R2 of 55,37%.
Keyword: distribution pattern, habitat characteristics, krangean (L. cubeba), Mountain Slamet, regeneration

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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