Judul: Tingkah Laku Beranak Induk Domba Garut yang Diberi Ransum Komplit Mengandung Minyak Biji Bunga Matahari
Abstrak: Garut ewes are small ruminants that have the prolific genetic potential to be developed as Good Farming Practice. The genetic potential should be supported by feed quality and balanced nutrition. Periparturient behavior is one of the important aspect related to livestock management, maintenance management and feeding management. This research aimed to study and observe garut ewes behavior of prepartus, partus and postpartus. The number of experimental animals were 11 garut pregnant ewes. The research treatments were of P0 (control), P1 ( 2% addition of sunflower seed oil), P2 (4% addition of sunflower seed oil), and P3 (6% addition of sunflower seed oil). This research used completely randomized design, the data were analyzed by ANOVA and significantly different would be continued by Tukey's test. The observed parameters were behavior of prepartus, partus and postpartus. The results showed that the addition with 2%, 4%, and 6% of sunflower seed oil which containing linoleic influenced the behavior prepartus and partus, but did not have any impact on behavior postpartus.
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Judul: Tingkah Laku Anak Domba Garut Setelah Lahir Yang Diberi Minyak Biji Matahari Dalam Ransum Induknya
Abstrak: Garut ewes are small ruminants that have the prolific genetic potential to be developed as Good Farming Practice. The genetic potential must be supported by a good quality of feed. Sunflower seed oil which has advantages as a source of linoleic fatty acids that can improve reproductive efficiency. Behavior is one of the important aspects related to maintenance management and handling of livestock that need to know the effect of feed to animal welfare. The purpose of this research was to identify and evaluate the lamb behavior of garut after born with addition sun flower seed oils. The number of observation were 18 of sheep. Experiment research consists of TL0 (control), TL4 (4% addition of sunflower seed oil), and TL6 (6% addition of sunflower seed oil). This research used completely randomized design, the data were analyzed by ANOVA, and significantly different data using Tukey test. Parameter behavior measured were standing first time, suckling first time, suckling duration first time, suckling frequency, vocal frequency, laying frequency, standing frequency, moving of tail frequency, and scramble nipples especially for twins. The results showed standing and suckling first time, vocal, standing, moving of tail was significantly affect (P<0.05) on treatment. The addition of sunflower oil by 4% and 6% affected behavior of lamb garut after born
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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