Judul: Study of Ecological Function of Plants and
Aesthetics in Open Areas of Two Office Buildings in South Jakarta
Abstrak: Ruang terbuka hijau di gedung perkantoran memiliki peran yang sangat penting karena keberadaannya berkaitan dengan aspek fungsi ekologis dan estetika kawasan. Namun, masih banyak RTH perkantoran di Jakarta yang pemanfaatannya belum maksimal. Penelitian ini bertujuan mengidentifikasi kondisi eksisting, menganalisis kenyamanan termal, audial, dan estetika di area sekitar Gedung TMT dan Sopo Del Tower berdasarkan pengukuran iklim mikro persepsi dan preferensi. Metode analisis yang digunakan di antaranya Key Performance Index, Temperature Humidity Index, Scenic Beauty Estimation, dan analisis statistik deskriptif dari persepsi dan preferensi pengguna. Hasil penelitian menunjukkan jumlah individu dan spesies pohon lebih banyak di Gedung TMT. Penilaian tanaman dalam fungsi modifikasi suhu, kontrol kelembapan udara, penahan angin, dan peredam kebisingan pada kedua lokasi termasuk dalam kategori sedang. Hasil perhitungan THI menunjukkan bahwa area terbuka Gedung TMT belum memenuhi kenyamanan termal yang ideal. Hasil penilaian untuk kualitas estetika lanskap Sopo Del Tower lebih tinggi dibandingkan dengan lanskap Gedung TMT. Responden cukup puas dengan kondisi eksisting kedua lokasi, namun masih perlu ada perbaikan dan penambahan fasilitas pada beberapa titik lokasi. Responden menyarankan adanya pemeliharaan yang lebih lagi untuk fasilitas, tanaman, dan selalu menjaga kebersihan lokasi., Green open space in office buildings has a very important role because its existence is related to aspects of ecological and aesthetic functions of the area. However, there are still many green spaces in Jakarta whose utilization is not optimal. This study aims to identify existing conditions, analyze thermal and aesthetic comfort in the area around Gedung TMT and Sopo Del Tower based on perceptions and preferences. The analysis methods used include Key Performance Index, Temperature Humidity Index, Scenic Beauty Estimation, and descriptive statistical analysis of user perceptions and preferences. The results showed that the number of individuals and tree species are more in Gedung TMT. The assessment of plants in the function of temperature modification, air humidity control, windbreak, and noise dampening at both locations is in the medium category. THI calculation results show that the open area of Gedung TMT has not met the ideal thermal comfort. The assessment results for the aesthetic quality of the Sopo Del Tower landscape are higher than those of the Gedung TMT landscape. Respondents were quite satisfied with the existing conditions of both locations, but there still needs improvements and additional facilities at several locations. Respondents suggested more maintenance for facilities, plants, and location cleanliness.
Keyword: fungsi ekologis, ruang terbuka hijau, fungsi estetika, gedung kantor

Judul: Evaluation of Ecological Functions
of Trees in Two Residential Complexes in West Bogor Sub-District
Abstrak: Perumahan di Kecamatan Bogor Barat terus mengalami perkembangan, termasuk Perumahan Pakuan Regency dan Sinbad Green Residence. Letak kedua perumahan yang strategis dan adanya berbagai aktivitas manusia yang mendorong munculnya berbagai masalah lingkungan, menyebabkan tingkat kenyamanan lingkungan menurun. Hal tersebut membuat RTH berperan penting pada lanskap perumahan. Pohon memiliki beragam fungsi ekologis, diantaranya sebagai modifikasi suhu (peneduh), kontrol kelembapan udara, penahan angin, dan peredam kebisingan. Kesesuaian pola penanaman dalam penataan tata hijau diperlukan agar suhu udara pada lanskap perumahan dapat lebih nyaman. Peningkatan suhu udara mempengaruhi kenyamanan termal bagi populasi yang terdapat pada wilayah tersebut. Tujuan penelitian ini melakukan evaluasi terkait fungsi ekologis pohon dan pola penanaman pada RTH kawasan lanskap perumahan serta menilai kenyamanan termal pada Ruang Terbuka Hijau perumahan tersebut. Tahap penelitian dilakukan melalui beberapa tahapan mulai dari tahap persiapan, inventarisasi, analisis, penilaian dan evaluasi, serta rekomendasi. Fungsi ekologis akan dianalisis dengan membandingkan kondisi eksisting dengan kriteria baku fungsi ekologis. Hasil evaluasi ini menunjukkan bahwa vegetasi di Cluster Jaya Dewata pada Perumahan Pakuan Regency unggul dalam hal modifikasi suhu (peneduh) dengan skor sebesar 257, penahan angin dengan skor sebesar 277, dan peredam kebisingan dengan skor sebesar 218. Vegetasi di Perumahan Sinbad Green Residence unggul dalam memenuhi fungsi pengontrol kelembapan udara dengan skor sebesar 263., Housing developments in West Bogor District continue to grow, including Pakuan Regency and Sinbad Green Residence. The strategic location of the two housing and the existence of various human activities that encourage the emergence of various environmental problems, causing the level of environmental comfort to decrease. This situation highlights the important role of green open spaces in the residential landscape. Trees have various ecological functions, such as temperature modification (providing shade), air humidity control, windbreaks, and noise reduction. Proper planting patterns are needed in green space planning to create more comfortable air temperatures in residential landscapes. Increased air temperature affects thermal comfort for the population in the area. The aim of this study is to evaluate the ecological functions of trees and planting patterns in the green open space of residential landscapes and assess the thermal comfort in the green open spaces of these housing areas. The research stages include preparation, inventory, analysis, assessment and evaluation, and recommendations. The ecological functions will be analyzed by comparing existing conditions with standard ecological function criteria. The evaluation results indicate that the vegetation in Cluster Jaya Dewata at Pakuan Regency excels in terms of temperature modification (providing shade) with a score of 257, windbreak function with a score of 277, and noise reduction with a score of 218. The vegetation at Sinbad Green Residence excels in fulfilling the air humidity control function with a score of 263.
Keyword: fungsi ekologis, kenyamanan termal, ruang terbuka hijau

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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