Judul: Dampak Perceraian dan Hubungan Sosial terhadap Kesejahteraan Psikologis Ibu sebagai Orang Tua Tunggal
Abstrak: Family with a single mothers who came from divorced, often bring the impact such as problem or change on a single mothers well-being. The aim of this research to indentify characteristics of single mother and relations with psychological well-being and social relations, and also analyze impact divorce on psychological well-being and social relations. Descriptive analysis, regression, and Pearson correlation was used in this study. The respondents were sixty single mother who come from the length divorced more than six month. The result showed that compare with present condition, psychological well-being, impact of divorce and social relation respondent are more significant lower than the first six month after divorce. There was relationship between age of present and divorce, family’s income, and size of family with level of depression, social support, and economic impact. Based on regression analysis we found there was negative effect of impact of divorce to psychological well-being at the first six month after divorce and the present. Other wise, we found there was not significant effect of divorce to social relationship.
Keyword: economic impact, level of depression, social support

Judul: Dukungan Sosial, Gaya Pengasuhan, dan Dampak Negatif Perceraian pada Anak Usia 7 – 15 Tahun
Abstrak: Divorce is one of child development risk factor that means positive parenting style are important roles to support child to addapt with the changes that were happened between before and after divorce. The aim of this research of was to analyze correlations of social support, parenting style, and the negative effects of divorce on 7-15 years old child of divorced families in Cianjur. The sample of this study were 7-15 years old child who had single parent and has been divorced at least one year and has not married again taken with nonprobability sampling techniques convenience as much as 60 people based at the research. The difference results show that is a significant higher of rejection parenting child after a divorce and feel a reduction in the negative impact of divorce on psychological and economy stats along with the distance to the present. The result of correlation test showed that there is a higher education of the mother, higher social support from parents, and higher of affective parenting were correlated with lower negative impact of divorce on economical, physical, and social stats of child.
Keyword: Children aged 7-15 years, divorce effects, parenting style, and social support.

Judul: Pc13-35s-Intron-Sma: Vektor Biner Fleksibel Untuk Ekspresi Gen Target Secara Konstitutif Pada Transformasi Tanaman Melalui Agrobacterium.
Abstrak: Teknik penyisipan gen target ke dalam tanaman menggunakan vektor alami Agrobacterium telah menjadi hal rutin dalam perakitan tanaman transgenik. Bakteri ini memiliki kemampuan dalam menyalin, memindahkan, dan menyisipkan daerah T-DNA ke dalam kromosom tanaman yang berada pada vektor biner. Vektor biner pC13-35S-Intron-Sma memungkinkan gen target yang disisipkan pada situs SmaI (CCCGGG) diatur ekspresinya oleh promoter kuat 35S dan terminator Nos untuk membentuk cassette ekspresi dengan cepat. Gen target akan mendapat tambahan intron (cat1) di ujung 5’- dan 6 kali histidin di ujung karboksil. Keberadaan intron untuk memastikan ekspresi gen target tidak berasal dari Agrobacterium. Komponen 6 kali histidin dapat berfungsi sebagai penanda untuk purifikasi dan deteksi pada tingkat protein. Untuk mengetahui keberhasilan dari konstruksi pC13-35S-Intron-Sma disisipkan gen enhanced green fluorescent protein (eGFP) pada situs SmaI membentuk pC13-35S-Intron-eGFP dan ditransformasi ke padi varietas Nipponbare menggunakan Agrobacterium EHA105. Infeksi dilakukan pada kalus yang berumur lima hari dan ditanam pada media kultivasi. Pengamatan kalus transforman yang berusia satu bulan setelah infeksi menunjukkan adanya sinyal ekspresi eGFP dengan terbentuknya pendaran berwarna hijau di bawah sinar biru. Hasil ini menunjukkan bahwa vektor biner pC13-35S-Intron-Sma dapat digunakan untuk transformasi tanaman dengan Agrobacterium.
Keyword: vektor biner, eGFP, intron, Agrobacterium, transformasi tanaman