Judul: Amplifikasi gen pup1 dan uji morfologi perakaran tanaman padi pada kondisi defisiensi fosfor
Abstrak: About 25% of Indonesia's land conditions has phosphorus deficiency. Its makes rice plants in Indonesia deficient of phosphorus. Genes mapping are adrift with P deficiency on chromosome 12 at the gene Pup1 rice. This research has been conducted on gene amplification of BC2F6 Pup1 on rice lines from backcross between Batur and Situ Bagendit with Kasalath and NIL-C443. The root morphology has been done observe the effectiveness of the cross. Root morphology test results by using a solution of Yoshida with 4 different treatment (0.5 P, 10 P, 0.5 P+45 ppm Al. dan 10 P+ 45 ppm Al). The results are tested by ANOVA test and Dunnet test. Based on the results of molecular analysis using primers K29-1 shows the overall results of crossbreeding plants have similarities with elders Kasalath and NIL-C443 as Pup1 donor. The ANOVA test and Dunnet’s results indicate the presence of root morphological differences between plants from crosses with P deficiency intolerant inheritence, Batur and Situ Bagendit.
Keyword: root morfology, gene Pup1, phosphor deficiency, NIL-C443, Kasalath

Judul: Konstitusi Background dan Karakter Fenotipik Galur-galur Toleran terhadap Defisiensi Fosfor dari Populasi BC2F6 Tanaman Padi (Oryza sativa L.)
Abstrak: Kemasaman, defisiensi P dan kekeringan merupakan masalah utama dalam pertanian padi di tanah Ultisol di Indonesia. Pengembangan galur padi yang toleran terhadap masalah tersebut diharapkan dapat mengurangi penggunaan pupuk P. Penelitian ini bertujuan untuk memverifikasi dan mengevaluasi galur-galur padi yang telah mendapatkan sisipan lokus Pup1. Penelitian ini terbagi dalam 3 percobaan, yaitu 1) Analisis konstitusi background, 2) Evaluasi populasi BC2F6 dibawah kondisi cekaman Al, dan 3) Pengaruh lokus Pup1 pada kondisi kekeringan. Penelitian ini dilakukan di Laboratorium Biologi Molekular dan Rumah Kaca Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian (BB Biogen) mulai November 2011 sampai Mei 2013. Analisis molekuler dilakukan dengan menggunakan seleksi foreground dan background, evaluasi cekaman Al menggunakan larutan hara Yoshida dengan rancangan petak terpisah, danevaluasi cekaman kekeringan menggunakan rancangan acak lengkap untuk uji PEG 8000 dan rancangan petak tepisah untuk uji DTA (daya tembus akar). Hasil evaluasi foreground menunjukkan bahwa lokus Pup1 telah terintegrasi, walaupun beberapa galur (SK5, SK6, SK7, SK8, SK9, SK10, SK19 dan SK20) menunjukkan integrasi yang tidak sempurna. Evaluasi background mengindikasikan bahwa proporsi maksimum pengembalian genom Situ Bagendit pada galur-galur turunan mencapai 95,7%. Evaluasi cekaman Al memperlihatkan bahwa pada kondisi kurang P dan cekaman Al, galur turunan SN lebih toleran dari galur turunan SK. Lokus Pup1 terlihat terekspresi dengan baik pada kondisi kurang P dan tanpa adanya cekaman Al. Beberapa galur toleran terhadap larutan PEG 8000 konsentrasi 20%. Pada percobaan DTA diperoleh satu galur turunan SK dan turunan SN yang daya tembusnya relatif sama dengan Cabacu (kontrol toleran kekeringan).
Keyword: Cekaman Al, defisiensi P, DTA, Kekeringan, Lokus Pup1, PEG 8000, seleksi background, seleksi foreground

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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