Judul: Analisis strategi pemasaran pariwisata bahari Taman Nasional Laut Kepulauan Seribu
Abstrak: Taman Nasional (TN) Kepulauan Seribu sebagai sebuah objek wisata bahari, kini dihadapkan pada situasi persaingan yang sangat keras. Pesaing yang menawarkan jasa pariwisata bahari semakin bertambah secara kualitas dan kuantitasnya.
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Judul: Analisis potensi wisata bahari di kawasan taman nasional kepulauan seribu, Propinsi DKI Jakarta dengan pendekatan recreation opportunity spectrum
Abstrak: Taman Nasional Kepulauan Seribu (TNKS) merupakan daerah konservasi yang sekaligus dimanfaatkan untuk wisata. Sebagai daerah konservasi TNKS memiliki tanggung jawab untuk menjaga keberlanjutan sumberdaya alam. Hal ini berarti bahwa TNKS harus merencanakan dan melakukan pengelolaan kawasan dengan benar melalui strategi dan kerangka pengelolaan yang menjamin dan mendukung keberlanjutan nilai-nilai sumberdaya alam. Recreation Opportunity Spectrum (ROS) yang digunakan dalam penelitian ini merupakan sebuah kerangka perencanaan melalui sistem zonasi pada landscape dan seascape. Tujuan dari ROS adalah untuk mendapatkan keseimbangan antara pemanfaatan dan konservasi. ROS memandang empat komponen pembentuk peluang rekreasi (recreation opportunity) adalah aktifitas, lokasi, pengalaman dan manfaat baik secara ekonomi maupun lingkungan.
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Judul: IPB (Bogor Agricultural University)
Abstrak: The use of Percoll gradients for Leydig cells isolation has been reported to influence cell toxicity and reduce the viability and the amount of living cells after culture. To dissolve this problem, study has been carried out used non toxic Nycodenz gradient to isolated and purified the Leydig cells. The use of Nycodenz gradient for the isolation and purification of Leydig cells has not been done by other researchers. To obtain the optimum condition of Leydig cells production, growth factor is required as supporting factors for development of growing cells. Therefore, hCG and ITS has been used in this study. Leydig cells conditioned medium (LCM) contained bioactive materials such as testosterone and growth factors. The Leydig cells conditioned medium as culture medium for bone marrow mesenchymal stem cells will be used to determine its influence to differentiation of cells. The phase of this study included: 1. Isolation and purification of Leydig cells using Nycodenz gradient : (I) with 3 column ( 5 % , 10 % , 15 % ) and ( II ) with 5 columns ( 4 % , 8 % , 10 % , 12 % , 15 % ) and Percoll gradient ( 21 % , 26 % , 34 % , 40 % , 60 % ) as a control . 2. In vitro culture of the best result from cell purification was cultured with the addition of hormones and growth factors : (1) DMEM+NBCS 10% as control, (2) DMEM + NBCS + 2.5 IU/ml hCG , (3) DMEM + NBCS+ 5 ug / ml insulin, 10 ug/ml transferrin, selenium 5 ug/ml (ITS ) and (4) DMEM +NBCS + hCG+ITS . 3 . The concentration of testosteron and protein analyzed from Leydig cells conditioned medium has been used for culturing bone marrow mesenchymal stem cells. The result of first phase study was Nycodenz gradient have the same purity and viability compared with Percoll gradient. After 3 days cultured, in Nycodenz gradient the concentration and viability of Leydig cells more higher than Percoll gradient. The combination of hCG and ITS in DMEM produced Leydig cell proliferation (90.64%) higher compared with hCG (86.99%), ITS (88.35%) and control (86.82%). Proliferation rate (population doubling time, PDT) from primary culture using combination of hCG and ITS showed the fastest (0.88 day) compared with ITS (0.97 day), hCG (1.02 day) and control (1.03 day). The same result of Leydig cells proliferation rate has also showed in first and second cell lines. Testosterone consentration analyzed was the highest (5.25 ng/ml) in DMEM combination with hCG and ITS compared with other treatments (hCG 5.06 ng/ml; ITS 3.19 ng/ml and control 2.46 ng/ml). Differentiation to became Leydig cells from bone marrow mesenchymal stem cells has been known from positive reaction to 3β-HSD staining. Added of hCG to Leydig cells conditioned medium was able to increased the number of Leydig cells to 71.9%. It was also showed that concentration of testosterone has been increased (0.93 ng/ml and 10.22 ng/ml) in the culture medium. The combination of hCG and ITS in DMEM had testosterone (0.71 ng/ml) and protein
Keyword: Leydig cells, rat, Nycodenz, testosterone, mesenchymal stem cells, bone marrow, conditioned medium, transdiferentiation