Judul: Shoot Proliferation and Pseudobulb Induction of Coelogyne pandurata Lindley Orchid on MS Medium with Addition of Coconut Water and Sugar
Abstrak: Coelogyne pandurata Lindley merupakan flora endemik khas Kalimantan Timur. Perbanyakan konvensional sulit dilakukan untuk pengadaan bibit berkualitas secara massal dalam waktu singkat sehingga dilakukan perbanyakan secara in vitro. Penelitian ini bertujuan mempelajari pengaruh pemberian air kelapa dan gula pada media MS terhadap daya proliferasi tunas dan pembentukan bulb anggrek hitam secara in vitro. Percobaan disusun dengan Rancangan Kelompok Lengkap Teracak (RKLT) faktorial dua faktor menggunakan bahan tanam planlet Coelogyne pandurata L. Faktor pertama adalah konsentrasi air kelapa: 0 (kontrol), 50, 100, 150, dan 200 ml/l; faktor kedua adalah konsentrasi gula: 0 (kontrol), 30, 40, dan 50 g/l. Hasil penelitian menunjukkan bahwa interaksi antara penambahan air kelapa dengan gula berpengaruh nyata meningkatkan jumlah tunas namun tidak nyata mempengaruhi parameter lainnya dengan jumlah rata-rata tunas terbanyak dihasilkan dari perlakuan air kelapa 150 ml/l dengan gula 40 g/l yaitu 1,70 tunas per eksplan. Perlakuan air kelapa 150 ml/l dengan gula 40 g/l menginduksi proliferasi tunas terbaik namun tidak berbeda nyata dengan beberapa perlakuan lain., Coelogyne pandurata Lindley is an endemic orchid from East Kalimantan. Conventional propagation is difficult to obtain quality seeds in a relative short time, so in vitro propagation is the best choice. This research aims to study the effect of coconut water and sugar on the shoot proliferation and pseudobulb formation of black orchids in vitro. The experiment was designed using a factorial two-factors Randomized Complete Block Design using the propagule of Coelogyne pandurata Lindley’s plantlet. The first factor is the concentration of Coconut Water (CW): 0 (control), 50, 100, 150, and 200 ml/l; the second factor is the sugar concentration: 0 (control), 30, 40, and 50 g/l. The results showed that the interaction of 150 ml/l CW with 40 g/l sugar had a significant effect on increasing shoot proliferation, namely 1.70 shoots per explant. Treatment of 150 ml/l CW with 40 g/l sugar induced the best shoot proliferation but was not significantly different compared with several other treatments.
Keyword: konsentrasi, sukrosa, Kalimantan Timur, sitokinin

Judul: Induction of Poliploid Diversity and  Regeneration Capability of Coelogyne fragrans Schltr. with Colchicine
Abstrak: Coelogyne fragrans merupakan anggrek yang berpotensi dikembangkan sebagai tanaman hias pot. Pemuliaan mutasi menggunakan kolkisin dapat meningkatkan keragaman genetik dan menginduksi poliploidi sehingga berpotensi meningkatkan kualitas karakteristik sifat agronomi tanaman. Penelitian ini bertujuan mempelajari respon C. fragrans terhadap konsentrasi kolkisin dan durasi perendamannya. Penelitian dilaksanakan dari bulan Mei hingga Agustus 2019 di Laboratorium Kultur Jaringan Tanaman II, Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor. Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) Faktorial dengan dua faktor, konsentrasi kolkisin yang terdiri dari 6 taraf: 0.0%, 0.02%, 0.04%, 0.06%, 0.08%, dan 0.10% dan durasi perendaman yang terdiri dari 2 taraf: 24 dan 48 jam. Pengamatan dan pengukuran dilakukan setiap minggu selama 12 minggu sejak eksplan ditanam pada media dasar ½ MS. Parameter yang diamati meliputi tingkat persentase hidup, persentase eksplan berploriferasi, jumlah tunas terbentuk dan jumlah akar terbentuk. Durasi dan konsentrasi kolkisin yang digunakan dalam penelitian ini berada dalam kisaran aman untuk persentase hidup eksplan. Perendaman 24 jam menghasilkan jumlah eksplan berproliferasi nyata lebih banyak dibandingkan perendaman 48 jam. Peningkatan konsentrasi kolkisin dari 0.02% hingga 0.10% tidak mempengaruhi persentase hidup eksplan dan jumlah tunas pada 12 minggu setelah kultur., Coelogyne fragrans is one of the orchid species that has the potential to be developed as potted flowering ornamentals. Mutation breeding using colchicine enhance the genetic diversity and to induce polyploidy, thus can potentially improve plant growth and flowering characteristics.The study was conducted to determine the responses of C. fragrans to different concentrations of colchicine and duration of immersion. The research was carried out from May to August 2019 at the Plant Tissue Culture Laboratory II, Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University. The design used in this study was a completely randomized design (CRD) with two factors, concentration of colchicine which consisted of 6 levels: 0.0%, 0.02%, 0.04%, 0.06%, 0.08%, and 0.10%, and duration of immersion which consists of 2 levels: 24 and 48 hours. Observations and measurements were conducted every week for 12 weeks since the explants were planted on MS base media. Parameters measured included survival percentage, percentage of p explant, number of formed shoots and number of formed roots. The duration and concentration of colchicine used in this study were within the safe range for the explant survival percentage. Immersion in colchicine for 24-hour resulted in significantly more number of explant proliferated than 48-hour immersion. Increasing the concentration of colchicine from 0.02% to 0.10% did not affect the percentage of explant survival and the number of shoots at 12 weeks after culture.
Keyword: kolkisin, mutasi, orchidaceae, proliferasi, tunas, colchicine, mutation, proliferation, shoots, Coelogyne fragrans

Judul: Implementasi Support Vector Machine (SVM) Untuk Klasifikasi Dokumen
Abstrak: Document classification is the process of grouping documents into specific categories that have been defined previously. In the real world, the distribution of the data is generally non-linear, which means the distribution of the data did not separate properly. Therefore we need a method that can classify documents that are non-linear. Support vector machine can classify documents that are non-linear with increasing dimension of the distribution of documents using kernel trick. This study will use a linear kernel for the classification of text documents. Results greatest accuracy in this study was 76% of 150 test documents with epsilon value 0.01. Factors affecting the results of the classification of which is the value of epsilon and length of test documents.
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