Judul: Pemodelan pengaruh jarak jangkau ruang terbuka hijau terhadap suhu permukaan di perkotaan (  studi kasus : kota Bogor )
Abstrak: This research was conducted in Bogor, located at 106o48'40'' E-106o46'22'' E and 6o30'53''S -6o40'08'' S. The research aimed at determining the influence of greenspace distance on micro climate especially on surface temperature, and therefore the effective distance of greenspace can be determined for urban spatial planning consideration. Urban surface temperature is the function of energy balance components and greenspace distance. Information of greenspace was derived from satellite image of Landsat 5 TM +.The image wa classified into seven land cover types i.e water bodies, constructed land, bush, paddy field (sawah), high vegetation, field (ladang), and oil palm. Distance to each greenspace type (bush, paddy field, high vegetation, field, and palm), were calculated using Euclidean distance principle. Energy balance components of each land cover class that include net radiation (Rn), soil heat flux (G), sensible heat flux (H), and latent heat flux (LE) were extracted from band 1,2,3 and 6 of Landsat 5 TM image data.
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Judul: Pendugaan Efek Pendinginan Menggunakan Model Empiris di Ruang Terbuka Hijau Institut Pertanian Bogor.
Abstrak: The cooling effect of small green areas with trees sites of various geometric configurations is the object of this study. It was studied experimentally at 3 different sites in Bogor Agricultural University during the period March-June 2013. An empirical model is developed in this study for predicting the cooling effect inside the sites. The model is based on statistical analysis carried out on 658 experimental observation gathered every 6 am and 2 pm from the 3 sites on calm days. Two factors that explain inside the sites are the partial shades area under the tree canopy and site specific effect. The cooling effect of green space areas on their immediate surrounding at noon was also analyzed. The cooling effect estimated in this study is perceivable up to about 50 m in the streets branching out from the site. The findings show the differences of every site, Graha Widya Wisuda (GWW) site that has most traffic outside the site shows biggest difference between site borders and the point measure in 50 m about -2.60 C.
Keyword: canopy, cooling effect, empirical study, green space area

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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