Judul: Vaksinasi Ikan Koi Menggunakan Vaksin DNA Anti- KHV dengan Dosis Berbeda
Abstrak: An effort to overcome the KHV outbreak that usually attack koi fish and common carp is administation of DNA vaccine, because DNA vaccines can stimulate specific immune responses and induced immunity in relatively high level, and safe to use. The aim of this research was to get the effective dose of DNA vaccine than can provide the best relative percents survival (RPS) and immunity. The treatments were vaccinated with different doses by the intramuscular injection technique, as follows: Treatment A (7.5 μg/100 mL), B (10 μg/100 mL), C (12.5 μg/100 mL), and the positive control (unvaccinated but were challenged by KHV) and negative control (unvaccinated and were not challenged by KHV). Results of this study showed that administation of the vaccine with different doses was able to provide survival value and RPS were not significantly different (p>0.05), the survival value of 97.22% and give RPS values of 95.8%. In addition based on the blood analysis, the result showed that the treatment dose of 12.5 mg/100 mL provides the best value in almost all parameters.
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Judul: Efektivitas Frekuensi Pemberian Vaksin DNA melalui Pakan terhadap Kelangsungan Hidup Relatif Ikan Mas yang Diinfeksi Koi Herpesvirus
Abstrak: Cultured of common carp is currently constrained by a koi herpesvirus (KHV) disease. KHV disease is highly malignant, infectious, and can kill common carp and koi over 80% of the population. It is therefore necessary to provide a prevention method that are safe and can be mass applied. In this study, it carried out anti-KHV DNA vaccination 1 mL/fish through artificial feed to determine the effective frequency of vaccination that produces a high survival on KHV infected fish. There are five treatments with three replicates, namely treatment A: vaccination once a week, treatment B: two times a week of vaccination, treatment C: vaccination three times a week, positive control: the fish were not vaccinated and challenged with KHV, and negative controls: fish were not vaccinated and injected with phosphate buffer saline solution. Feeding was performed 2 times a day at satiation. The results showed that vaccination three times a week offered 4 the highest relative percent survival (84.6%) and showed the best phagocytic activity compared with other treatments. Hence, vaccination via the feed can effectively increase the survival of fish, and this can be as an alternative method to control KHV infection in carp and koi culture.
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Judul: Kegiatan merencana dan melaksana dalam arsitektura landsekap
Abstrak: Permasalahan yang timbul dalam abad modern sekarang ini sering disebabkan oleh tingginya laju pertambahan penduduk di suatu negara. Di negara yang sedang berkembang laju pertambahan penduduknya justru lebih rendah jika dibandingkan dengan negara-negara maju. Data dari USDA (1975) dalam Khumaidi (1980) menunjukkan bahwa laju pertambahan penduduk di negara sedang berkembang sebesar 2.5% per tahun, sedangkan di negara maju hanya mencapai 1% per tahun. Dengan demikian jelas bahwa permasalahan yang timbul akibat pertambahan penduduk lebih sering dirasakan di negara-negara yang sedang berkembang. Pesatnya pertambahan jumlah penduduk selain menimbulkan masalah ketenagakerjaan juga telah mengakibatkan makin menyempitnya total areal pemukiman per keluarga (Nurhajati, 1983). Keadaan ini terlihat jelas di kota-kota besar seperti kota metropolitan Jakarta, Surabaya dan Semarang. Banyak. rumah yang mempunyai halaman sempit di kota tersebut. Kondisi lingkungan yang demikian tentunya bukanlah kondisi ideal yang diinginkan oleh manusia. Kesegaran udara, kenyamanan lingkungan dan ketenteraman tidak dapat diperoleh dalam lingkungan yang demikian. Oleh karena itu tidak mengherankan bila pada hari-hari libur sering orang-orang dari kota besar menyempatkan diri berekreasi ke luar kota hanya sekedar untuk menikmati sejuknya udara pegunungan dan indahnya pemandangan alam yang sudah tidak dapat diperoleh lagi di kota-kota besar.
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