Judul: Intensitas Penyakit Penting, Deteksi Huanglongbing Dan Pengaruh Aplikasi Pgpr Pada Tanaman Jeruk Di Kabupaten Bogor
Abstrak: Jeruk adalah salah satu tanaman holtikultura penting dan mempunyai nilai ekonomi tinggi di Indonesia. Salah satu permasalahan penting yang dihadapi dalam budidaya jeruk adalah adanya penyakit tanaman yang mengakibatkan penurunan kualitas dan kuantitas hasil. Tujuan penelitian ini adalah mengamati penyakit-penyakit umum tanaman jeruk di Kabupaten Bogor, Provinsi Jawa Barat, deteksi penyakit huanglongbing dan melihat pengaruh aplikasi plant growth promoting rhizobacteria (PGPR) pada fase vegetatifnya. Tahapan penelitian meliputi pengamatan intensitas dan identifikasi penyakit yang ditemui di lapang, aplikasi PGPR pada tanaman jeruk fase vegetatif untuk mengetahi pengaruhnya terhadap intensitas penyakit jeruk. Selanjutnya, pengamatan berdasarkan peubah agronomi tanaman yang dilakukan setiap minggu selama 10 minggu pengamatan. Khusus untuk penyakit huanglongbing, yang juga dikenal sebagai CVPD, dilakukan pengamatan gejala eksternal serta gejala internal melalui uji akumulasi pati pada jaringan floem yang diambil dari tulang daun tanaman jeruk dan deteksi secara molekuler dengan teknik polymerase chain reaction (PCR). Berdasarkan pengukuran Area􀀃���������􀀸���������nder􀀃���������􀁗���������􀁋���������􀁈��������� 􀀧���������isease􀀃���������􀀳���������rogress 􀀦���������urve (AUDPC) ditunjukkan bahwa penyakit yang paling dominan pada fase vegetatif dan generatif adalah embun jelaga (Meliola sp.) dan penyakit kudis (Elsinoe fawcetti). Aplikasi PGPR memberikan perubahan tidak signifikan terhadap perubahan intensitas penyakit dibandingkan dengan tanaman jeruk yang tidak diberi perlakuan, tetapi dapat mempengaruhi pertumbuhan dan perkembangan tanaman jeruk. Penyakit huanglongbing ditemukan dalam insidensi yang rendah. Diagnosis huanglongbing dari gejala internal ditunjukkan dengan adanya akumulasi pati pada jaringan floem, namun tidak selalu memberikan hasil uji PCR yang positif.
Keyword: CVPD, huanglongbing, penyakit jeruk, PGPR, polymerase chain reaction

Judul: Optimasi Deteksi Penyakit Huanglongbing pada Tanaman Jeruk Menggunakan Teknik Polymerase Chain Reaction
Abstrak: Citrus is an important horticultural commodity with high economic value in many countries. Huanglongbing disease, or in Indonesia known as citrus vein phloem degeneration caused by Liberobacter asiaticus bacterium, is a major factor limiting citrus production in many Asian countries. The disease detection and diagnosis solely based on its symptom is sometimes not reliable since it resembles to those caused by nutrient deficiencies. Detection using polymerase chain reaction is an accurate technique for many plant pathogens including the huanglongbing causal agent. The research objectives are to observe huanglongbing disease of citrus, based on external and internal symptoms, in Bogor and to optimize PCR technique for detection of the pathogen. The disease identification and detection based on the external symptom showed that there was less specific character which correlated with pathogen presence in the sample. Symptom of the infected plants is leaf discoloration and deformation. The leaves showing symptoms of chlorosis, are smaller and more lancet than leaves without chlorosis symptoms. The results of detection based on internal symptom showed as starch accumulation in phloem are consistent with that of PCR. The optimization of detection using PCR showed that the most effective and efficient extraction method to obtain DNA template was a modification of Dellaporta et al. (1986) with extraction buffer concentration at 2 times. After 2 weeks period of storage treatment at 4 oC, Huanglongbing disease can still be detected by PCR, but its DNA bands showed inconsistency among three replications. The most optimum two weeks sample preservation is at 4 oC, whereas preservation with CTAB buffer still maintain the sample to yield DNA template adequately for PCR
Keyword: PCR., huanglongbing, DNA extraction, detection, CPVD

Judul: Sintesis dan Pencirian Natrium Titanat (Na2Ti2O4(OH)2) Berbahan Dasar Titanium Dioksida
Abstrak: TiO2 can be utilized as basic material for synthesis of sodium titanate (Na2Ti2O4(OH)2). This research was purposed to synthesize and characterize sodium titanate from TiO2 rutile powder as a basic material. Sodium titanate was synthesized by hydrothermal process at temperature 130 °C by mixing TiO2 rutile powder with 5 M and 10 M NaOH solution, then each was calcinated at temperature 700 °C. The energy dispersive X-ray analysis showed that sodium titanate has been successfully synthesized. This result was also supported by functional group analysis with Fourier transform infrared (FTIR) spectrofotometer showing the existence of Ti-O-Na vibration band at 989.42 cm-1. The formation of sodium titanate was depend on the reaction condition such as temperature, reaction time, and NaOH concentration. Scanning electron microscope image indicated that sodium titanate synthesized at 130 °C in 20 hours reaction time with 10 M NaOH had crystal size of 99.37 nm.
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