Judul: Inhibition Mechanism of Atherosclerosis Initiation at Cellular Level by Curcuminoid of Temu Mangga Extract (Curcuma mangga).
Abstrak: Temu mangga (Curcuma mangga) is a potent medicinal herb which functions as an antioxidant because of its curcuminoid content. The curcuminoid has been shown to reduce the susceptibility of low-density lipoprotein (LDL) to oxidation. The oxidation of LDL is believed to be the initiating factor for the development and progression of atherosclerosis. An adhesion molecule is a protein that is expressed to the cell surface when disturbed endothelial function. Proteoglican (PG) is an extracellular matrix found on the cell surface of blood vessels and PG could change in association at development of atherosclerosis. The study aims to assess the potential of the curcuminoids extract inhibiting LDL oxidation and expression of an adhesion molecules on endothelial surface and the role proteoglycan to LDL retention in vitro. The LDL was harvested and isolated from five adult male Macaca fascicularis fed an aterogenic diet for three months. Curcuminoid compound was isolated using ethanol and water solouble.Smooth muscle cells isolated from white rat’s aorta coronary. Macrophages was isolated from peritoneal of mice and monocyte of M nemestrina. Analyses the oxidation LDL were done by measuring the formation of thiobarbituric acid reactive substance (TBARS) as malonaldehyde (MDA). The fractions of curcuminoid was analized using HPLC. An adhesion molecules was examined immunohistochemical staining that reacted with anti VCAM-1 antibody and anti ICAM-1 antibody. The measured was hexaronat acid using HPLC method and then converted as proteoglican. The results showed as 1.84% curcuminoids from yield of rhizomes temu mango. The quantitative analysis showed is curcuminoids extracts consist of curcumine (6.2%), demetoxi-curcumine (2.3%) and bis-demetoxi curcumine (3.0%). The result of inhibiton of LDL oxidation in macrophage of mice and M. nemestrina were curcuminoid at eight ppm for four hours and six hours incubation (P<001). These data suggest that curcuminoid extract of temu mangga was able to inhibit LDL oxidation to macrophages of mice and Macaca nemestra. There was inhibited of 13.068% of oxidation LDL at eight ppm four hours incubation to macrophages of mice compared control without curcuminoid. Inhibiton of oxidation LDL at macrophages of M. nemestrina by curcuminoid at eight ppm for six hours incubation there was 24.282%. The results of adhesion molecule of ICAM-1 molecules can be expressed with brownish yellow color, whereas the VCAM-1 molecules did not expressed on the cell surface. The result of hexarunate acid could not be detected.
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Judul: Aktivitas Inhibisi Enzim α-Glukosidase dan Sitotoksisitas Ekstrak Kurkuminoid Rimpang Temulawak dari Berbagai Aksesi (In Vitro)
Abstrak: Temulawak (Curcuma xanthorriza Roxb.) is one of the Zingiberaceae family medicinal plants that grow in Indonesia. Temulawak rhizome contains curcuminoid which useful as an antidiabetic. This research aims to get temulawak accession with high capability of the inhibition activity of the α-glukosidase enzyme and it’s potential as a compound that toxic to shrimp’s larval Artemia salina Leach. Antidiabetic activity has been measured by formation of p-nitrophenol from enzymatc reaction of nitrophenil-α-D-glucopyranose (p-NPG) which is catalyze by α-glucosidase enzyme. The formation of p-nitrophenol was measured by spectrophotometer at λ=410 nm. The results of curcuminoid content and bioactivity assay (α-glukosidase enzyme inhibition and cytotoxicity) showed that the best accession of temulawak is Wonogiri’s accession. The value of curcuminoid content, IC50, LC50, from Wonogiri’s accession of temulawak rhizome extract, respectively for 85.19±4.64 mg/g, 333±22.52 μg/mL, and 82.78±7.70 μg/mL. Wonogiri’s accesion has the potential to be further researched and developed as a new variety.
Keyword: Bogor Agricultural University (IPB), cytotoxicity, curcuminoids, antidiabetic, Curcuma xanthooriza

Judul: Pemanenan Energi Listrik dari Kincir Angin Vertikal
Abstrak: Usaha pemanenan energi angin di Indonesia masih sedikit. Sampai Tahun 2016 hanya 3.54 MW alat pemanenan energi angin yang sudah terpasang. Kendala utamanya ialah dibutuhkan investasi yang besar untuk membuatnya. Pembuatan kincir angin dengan ukuran yang lebih kecil dengan bahan yang dapat dibeli di pasar lokal menjadi solusi agar pembuatan kincir angin lebih ekonomis. Pembuatan kincir vertikal tipe Savonius dapat menjadi solusinya. Kincir vertikal Savonius dapat beroperasi pada kecepatan < 3 m/s dan ketinggian < 50 m. Kincir angin ini terdiri dari tiga sistem, yaitu generator, rotor kincir dan transmisi pulley-belt. Generator dibuat dari modifikasi motor AC dan magnet neodymium. Rotor kincir dibuat dari pipa PVC 4”, pipa alumunium ¾”, dan papan polikarbonat. Transmisi pulley-belt dibuat dari plastik dan karet. Kincir ini membutuhkan gaya minimal 3 N untuk dapat berputar atau torsi minimalnya 0.75 Nm. Kecepatan angin yang dibutuhkan untuk merotasikannya sebesar 1.9 m/s. Daya maksimal yang dapat dihasilkan ialah 5.76 watt berupa tegangan sebesar 36 volt dan arus 160 mA.
Keyword: energi terbarukan, generator, motor AC, sudu Savonius