Judul: Studi Morfologi dan Molekuler Boesenbergia angustifolia (Hallier f.) Schltr. dan Boesenbergia sp.
Abstrak: Zingiberaceae is the largest family of Zingiberales with 53 genera, over 1200 species and spread in the tropics. Boesenbergia is the one of Zingiberaceae genera, that has not been explored, and therefore, in this research morphological and phylogenetic studies were conducted using Boesenbergia angustifolia and Boesenbergia sp. of Sumatra. The method used in this study is the observation of morphological characters and molecular characters in based of variation in Internal Transcribed Spacer (ITS) sequence are used for phylogenetic tree construction. DNA sample was isolated using cetyltrimethylammonium bromide based method of Doyle & Doyle modification. Amplification of ITS region resulted in ± 800 bp amplicon. Sequencing DNA of ITS region resulted in ± 788 bp amplicon from Boesenbergia angustifolia and ± 789 bp amplicon from Boesenbergia sp. Recontruction of phylogenetic tree was analysed using the Phylogenetic Analysis Using Parsimony program. The morphological characters of Boesenbergia angustifolia and Boesenbergia sp have similar flowers and inflorescence character. Suggestion that they are of the same genera. This is also supported by ITS DNA sequence, both of these plant species have 17 bases difference. Phylogeneticly, these plant species also form branching close to the high level of confidence with 97% bootstrap value. They are of the same veriety.
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Judul: Studi Molekuler untuk Menentukan Kekerabatan Genus Zingiber Varietas Zerumbet
Abstrak: Problems due to opinion differences in medicinal plants classification cause confusion on the use of scientific name of plants. The grouping of plants which based on morphology in some cases is less effective and it is necessary to perform molecular studies used maturase (matK) and Internal Transcribe Spacer (ITS) as molecular markers. The study was aimed to classify members of the Zingiber genus Zerumbet subsection that is Zingiber zerumbet, Zingiber aromaticum, and Zingiber amaricans. Stages of research were isolation of DNA, DNA amplification, DNA sequencing, alignment of DNA sequences, and construction of phylogenetic tree. Results showed that the isolated DNA was of good quality. The size of the amplified DNA with the matK was 2000–3000 bp, while the size of the amplified DNA with the ITS was 900 bp. ITS sequences were of low quality, so that only the matK sequences were used for inference. Based on the results of sequence alignment with data obtained from GenBank differences between members of the genus Zingiber subsection Zerumbet with other types of Zingiber was 1–11 bp and the phylogenetic tree with consistency index (CI)= 0.897 and retention index (RI)= 0.833 shows a very close genetic relationship between the three members of the Zingiber genus Zerumbet subsection with bootstrap (BS)= 59. The third member of the Zingiber genus Zerumbet subsection is spesies kompleks which is under the type of Zingiber zerumbet.
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Judul: Feeder kelinci ternak otomatis menggunkan prinsip aktuator solenoid
Abstrak: Feeder kelinci ternak otomatis merupakan prototipe yang diciptakan dengan pengaturan distribusi pakan berbentuk pellet pada konveyor sekrup termodifikasi dengan tambahan pintu akses teraktivasi oleh aktuator solenoid. Mikrokontroler Arduino Uno R3 pada prototipe ini digunakan untuk proses perhitungan jarak pintu akses, waktu tempuh pakan, dan kecepatan distribusi pakan. Prototipe ini bermanfaat untuk para pembudidaya/peternak kelinci sebagai inovasi teknologi dalam kegiatan pemeliharaan kelinci secara moderen. Pakan kelinci diatur melalui rotasi motor stepper pada penakar pakan sehingga dapat mengukur porsi pakan secara akurat. Penggunaan motor DC dilengkapi gearbox bertujuan mengendalikan konveyor sekrup agar berputar dengan konstan sehingga menghasilkan gaya dorong pada pakan yang akan didistribusikan. Hasil perhitungan kecepatan distribusi pakan menentukan waktu aktivasi aktuator solenoid yang akan membuka pintu akses pakan untuk keluar dari konveyor sekrup.
Keyword: Aktuator solenoid, feeder, penakar pakan, konveyor sekrup