Judul: Pengaruh Parental Self Efficacy dan Praktik Pengasuhan terhadap Perilaku Agresif pada Anak Prasekolah
Abstrak: Perilaku agresif merupakan perilaku yang dimaksudkan untuk melukai orang lain, hewan, dan benda. Penelitian ini bertujuan untuk menganalisis faktor-faktor yang memengaruhi perilaku agresif. Penelitian ini melibatkan 36 keluarga dengan anak usia prasekolah yang dipilih secara purposive. Kriteria contoh pada penelitian ini adalah ibu sebagai pengasuh utama dan memiliki anak bersekolah di PAUD Mawar dan Nurul Fajriah, Desa Ciasmara, Kecamatan Pamijahan, Kabupaten Bogor. Data dikumpulkan dengan teknik wawancara menggunakan kuesioner. Data dianalisis menggunakan uji Chi-square, uji korelasi Spearman, uji korelasi Pearson, dan regresi linear berganda. Hasil uji korelasi Pearson menunjukkan bahwa parental self efficacy berhubungan negatif signifikan dengan praktik pengasuhan negatif dan perilaku agresif. Sementara itu, praktik pengasuhan negatif memiliki hubungan positif signifikan dengan perilaku agresif. Penelitian ini juga menemukan pengaruh parental self efficacy dan praktik pengasuhan negatif terhadap perilaku agresif anak.
Keyword: anak usia prasekolah, parental self efficacy, perilaku agresif, praktik pengasuhan.

Judul: Pengaruh Pengasuhan Penerimaan-Penolakan dan Kelekatan Ibu-Anak terhadap Karakter Disiplin Anak Usia Prasekolah.
Abstrak: Penelitian ini bertujuan untuk menganalisis pengaruh pengasuhan penerimaan-penolakan, kelekatan ibu anak terhadap karakter disiplin anak usia prasekolah. Kriteria contoh pada penelitian ini adalah ibu sebagai pengasuh utama dan memiliki anak yang bersekolah di PAUD Mawar dan Nurul Fajriah, Desa Ciasmara, Kecamatan Pamijahan, Kabupaten Bogor. Penelitian ini melibatkan 36 keluarga dengan anak usia prasekolah yang dipilih secara purposive. Data dianalisis menggunakan uji chi-square, uji korelasi Pearson dan regresi linear berganda. Pengasuhan penerimaan dan karakter disiplin anak prasekolah termasuk dalam kategori rendah. Kelekatan yang terjalin antara ibu dan anak terkategori cukup baik. Terdapat hubungan positif signifikan antara kelekatan ibu-anak dengan pengasuhan penerimaan dan karakter disiplin. Hasil uji regresi linear berganda menunjukkan bahwa lama pendidikan ibu berpengaruh negatif terhadap karakter disiplin anak, sedangkan pendapatan perkapita dan kelekatan ibu-anak memiliki pengaruh positif terhadap karakter disiplin anak.
Keyword: Anak usia prasekolah, Karakter disiplin, Kelekatan ibu anak, Pengasuhan penerimaan-penolakan

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable