Judul: Modeling Land-Use Change and Its Driving Factors (A preliminary dynamic landscape-based model of Sumberjaya Watershed
Abstrak: The prototype land-use model in this research is intended to provide support for negotiation making processes with information on the dynamics of land use change under some combinations of economic scenarios. The main characteristic of the model is to integrate economic and spatial dynamics. A combination of system dynamics and constrained cellular Automata simulates changes in land use driven by economic factors. At its current status, it provides insight of past and simulated land use changes based on economic scenarios.
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Judul: Modeling of Flood for Land Use Management (Case Study of Ciliwung Watershed)
Abstrak: Floods are one of the major disasters affecting many countries in the world year after year. It is an inevitable natural phenomenon occurring from time to time in all rivers and natural drainage systems. It causes damage to lives, natural resources and environment as well as the loss of economy and health. Floods represent complex problems because of its variety. Therefore, this variety cannot be studied or controlled only by one or two specific methods. The objectives of this research are to understand the process of flood events and its interaction with hydrometeorological components, to develop flood model for watershed management and to determine the effect of land use change to watershed discharge which indicates flood event. The research consists of four processes those are 1) Data Preparation, 2) Model Development, 3) Model Simulation and 4) Model Calibration and Validation. Data preparation was conducted for two kinds of data namely spatial data and tabular data. Model developed as numerical model of the hydrology of a river basin system. This model includes the response of watershed to precipitation, the actions of the river network as water flows through the river, the effect of land use changes, and the effect of engineering structures to the watershed. Model simulated by change land use as an input. Model calibrated by using water level data of field measurement in year 1996 and model validated by using water level data of field measurement in year 2000. The result of this reseach showed how the process based modeling is useful to model hidrological processes over the watershed. Precipitation is the main input for hidrology simulation and land use change gives the effect of water level in the watershed. The model which is built in this research can be used to evaluate runoff from different land use areas. For assessing the hydrological effects of land use changes on floods, three hypothetical scenarios, namely urbanization, deforestation and afforestation scenario, were considered made. It was found from the model simulation that the urbanization scenario has the higest negative impact on increasing water level. Deforestation has the second largest negative impact, while afforestation causes positive impact shown by decreasing water level. The result indicated that the urbanization produce the highest peak flow, followed by the deforestation and afforestation scenario. The simulated water level for the present land use is 84 cm, for the urbanization scenario 135 cm, deforestation scenario 92 cm, and afforestation scenario 59 cm. Accordingly, the urbanization scenario increases the peak discharge by 61%, the deforestation scenario increase 15%, and afforestation decrease by 19%.
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Judul: Influenza Specific Immunoglobulin Y Purification from Egg Yolk with Sodium Chloride Precipitation Method
Abstrak: Imunoglobulin Yolk (IgY) yang terdapat dalam kuning telur telah banyak dimanfaatkan untuk imunoterapi dan imunodiagnostik. Salah satu kendala dalam pemurnian IgY dari kuning telur adalah pemisahan Ig dengan lemak, sehingga teknik pemurnian Ig selalu berkembang. Penelitian ini bertujuan untuk melakukan pemurnian IgY dari kuning telur dengan metode pengendapan menggunakan sodium chloride (NaCl) serta melakukan karakterisasi IgY spesifik influenza. Sodium chloride dipilih karena bahan kimia ini relatif murah, ramah lingkungan, dan tidak merusak imunoaktivitas IgY. Kuning telur yang digunakan dalam penelitian ini berasal dari telur ayam layer yang divaksinasi dengan vaksin influenza Flubio® yang berisi virus Influenza A/H3N2, A/H1N1 dan B/Washington. Pemurnian IgY hanya dilakukan pada kuning telur yang mengandung antibodi spesifik influenza. Deteksi keberadaan antibodi spesifik influenza ditentukan dengan uji agar gel presipitasi (AGP) dan metode enzyme linked immunosorbent assay (ELISA). Konsentrasi IgY hasil pemurnian dihitung dengan spektrofotometer dan karakterisasi protein dilakukan dengan metode Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Konsentrasi protein dari hasil pemurnian IgY memiliki nilai rata – rata 50,403 mg/mL. Hasil SDS-PAGE menunjukkan enam pita protein, yaitu struktur IgY utuh (174,63 kDa), Apoprotein-LDL (129,77 kDa), rantai berat IgY (66,54 kDa), Fab ( 47,65 kDa), rantai ringan IgY (21,85 kDa), dan β-livetin (36,74 kDa). IgY hasil pemurnian masih dapat berikatan dengan antigen influenza berdasarkan uji ELISA. Hasil penelitian ini menunjukkan bahwa pemurnian IgY menggunakan metode pengendapan NaCl dapat memurnikan IgY dengan struktur utuh, meskipun masih ada dua protein tidak spesifik hasil pemurnian. IgY yang dapat berikatan dengan antigen influenza mengindikasikan prospek pemanfaatannya untuk pencegahan influenza dan imunodiagnostik., Yolk immunoglobulin (IgY) contained in egg yolk has been widely used for immunotherapy and immunodiagnostics. One of the obstacles in IgY purification from egg yolk is separating Ig from fat, so that IgY purification techniques are always developing. This study aimed to purify IgY from egg yolk using sodium chloride (NaCl) precipitation method and to characterize influenza-specific IgY. Sodium chloride was chosen because this chemical is relatively unexpensive, environmentally friendly, and does not impair IgY immunoactivity. The egg yolks used in this study were collected from layer eggs vaccinated with the Flubio® influenza vaccine, which contains the Influenza A/H3N2, A/H1N1 and B/Washington viruses. IgY purification was only carried out on egg yolks containing influenza specific antibodies. The presence of influenza-specific antibodies was determined by the agar gel precipitation (AGP) test and the enzyme linked immunosorbent assay (ELISA) method. The purified IgY concentration was determined using spectrophotometer, and protein characterization was performed using the Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) method. Protein concentration from purification has a mean value of 50,403 mg/mL . SDS-PAGE result showed six protein bands, namely intact IgY structure (174,63 kDa), Apoprotein-LDL (129,77 kDa), IgY heavy chain (66,54 kDa), Fab (47,65 kDa), IgY light chain (21,85 kDa), and β-livetin (36,74 kDa). The purified IgY still able to bind with influenza antigen based on the ELISA test. The results of this study indicate that purification of IgY using the NaCl precipitation method can purify IgY with intact structure, although there are still two unspecific proteins in the purification results. The resulting IgY can bind to influenza antigens indicating the
Keyword: Immunoglobulin Y, influenza, egg yolk, purification, sodium chloride