Judul: Suatu Study Klasifikasi Dua Sub Sektor Industri di Propinsi Jawa Barat
Abstrak: Tujuan penelitian ini adalah untuk mencari indikator- indikator yang dapat dipakai sebagai dasar pembuatan klasi- fikasi industri baik untuk satu sub sektor maupun sub-sub sektor industri lainnya. Sub sektor industri yang ditelaah adalah industri makanan, minuman dan tembakau serta indus- tri tekstil, pakaian jadi dan kulit. Berdasarkan analisis komponen utama, dua jenis perusa- haan industri tersebut dibagi dalam tiga kelompok berdasar- kan selang kepercayaan rata-rata skor komponen utama perta- ma atau kedua. Dengan menggunakan analisa gerombol dan kemudian ana- lisis diskriminan, dua sub sektor perusahaan industri ter- sebut juga dibagi dalam tiga kelompok. Setelah diadakan penyederhanaan jumlah peubah yang digunakan dalam fungsi diskriminan, peubah jumlah tenaga kerja dan jumlah upah atau gaji dapat dipakai sebagai indikator pengklasifikasian. Jika digunakan tiga peubah sebagai indikator, maka pe- ubah jumlah tenaga kerja, jumlah upah atau gaji dan jumlah PK tenaga kerja mekanik terpilih untuk digunakan. Banyaknya indikator yang dianggap menghasilkan pengelompokan terbaik terdiri atas empat peubah ...
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Judul: Studi pembentukan lapisan perusahaan industri pakaian jadi di propinsi Jawa Barat
Abstrak: Tujuan penelitian ini adalah untuk menentukan peubah yang layak dijadikan dasar pelapisan, menentukan titik batas pelapisan optimum, dan menentukan ukuran contoh optimum. Di samping itu diteliti pula sebaran karakteristik-karakteristik populasi, dan tingkat efisiensi teknik penarikan contoh yang ditetapkan dalam penelitian ini. Penelitian ini dilakukan dengan meneliti karakteristik pokok industri pakaian jadi yang berlokasi di wilayah Barat. Jawa Karakteristik pokok yang diteliti meliputi: jumlah tenaga kerja, nilai upah, nilai bahan baku dan bahan peno- long, nilai bahan bakar dan listrik, nilai pengeluaran lain, dan nilai keluaran. Data yang digunakan dalam penelitian ini adalah merupakan hasil Survai Industri Besar/Sedang Tahunan yang dilaksanakan oleh Biro Pusat Statistik melalui Perwakilan-perwakilan Cabangnya pada tahun 1988. Dengan memanfaatkan ringkasan-5 angka dan ringkasan-3 angka dapat diketahui, bahwa sebaran keenam karakteristik populasi menjulur ke arah kanan Ini berarti, bahwa terdapat frekuensi yang tinggi pada nilai-nilai rendah, akan tetapi terdapat pula yang sangat besar. ..
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Judul: Development of DNA Vaccine Encoding Glycoprotein of Koi Herpes Virus Using Local Isolate.
Abstrak: The aim of this research was to develop DNA vaccine of Koi Herpesvirus in carp. Glycoprotein gene originated from ORF 25 KHV virus that is 1.8 kbp in size, is a potential gene in DNA vaccine construction. The sequencing and similarity analysis of the gene using BLAST software showed that this gene is a GP25 gene and was found to be very similar (99%) to GP25 of KHV virus from the USA and Israel. When this GP25 gene was ligated with pGEMT Easy (pT, 3 kbp in size) vector, plasmid size was increasing up to 5 kb. Following this, a digestion of pT-GP25 plasmid using Sal I enzyme was applied resulted in a GP25 fragment (1.8 kbp). Subsequently, pAct-D6 with a size of 8.5 kbp was also cut using the same enzyme to eleminate a D6 gene with a size of 1.6 kbp. GP25 gene was then ligated with pAct resulted a pAct-GP25 plasmid with a size of 8.8 kbp. As there was only one restriction site applied, ligation can be occurred in two different directions. Thus, in order to determine the plasmid with the desired direction PCR analysis was carried out, and plasmid with the right direction was the one that sized approximately 2.1 kbp (no. 6, 17 and 20). High level of GFP gene expression was detected in all tissues analyzed, i.e. kidney, gil, spleen, and muscle 24 hours after injection which indicated that β-actin promoter of Japanese medaka could be active in common carp. Although not as high as on 24 hours, expression was still detected until day 7 and disappeared after day 28 post injection indicating that pAct-GFP can be remained and β-actin promoter of Japanese medaka can be active in muscle, gills and kidney at least a week after injection. The result of RT-PCR analysis on pAct-GP25 injected common carp showed that GP25 gene expression can be detected 14 days after injection in all tissues observed. The expression level differences between GFP and GP25 genes possibly related to the primer sensitivity on annealing process to cDNA template and the size of PCR targeted DNA fragment, as smaller PCR targeted product will be easier to be amplified than a bigger one. The length of PCR targeted DNA with GP25 primer was 1.8 kbp whereas GFP gene was 0.6 kbp.The challenge test on common carp results showed that all unvaccinated and negative control (not infected with KHV) was alive until the end of the experiment. Whereas, the survival of fish vaccinated with a dose of 12.5 was ranged from 60.9 and 96.7%. In relation to the activity of β-actin promoter that was used in this DNA vaccine, gene expression was occured 24 hours after vaccination and remained until 2 weeks. The control fish that was not injected by GP25 showed no GP25 expression either in the observed tissues or organs and resulted in a lower survival, i.e. 23.3%. The challenge test results has indicated that the fish vaccinated with GP25 at doses of 7.5 and 12.5 μg/100μL were able to produce KHV glycoprotein in their body which would be
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