Judul: Morfologi dan Karakteristik Mineral Tanah pada Lahan Sawah di Desa Cijedil dan Desa Cibeureum Kecamatan Cugenang Kabupaten Cianjur, Jawa Barat
Abstrak: Penelitian ini bertujuan untuk mengetahui jenis morfologi dan karakteristik mineral tanah sawah dan hubungannya dengan tingkat pelapukan dan perkembangan tanah serta kesuburan alami tanahnya. Penelitian ini dilakukan di dua desa yaitu Desa Cijedil dan Desa Cibeureum Kecamatan Cugenang Kabupaten Cianjur, Jawa Barat dengan melakukan pendeskripsian profil dan pengambilan contoh tanah untuk dianalisis sifat kimia dan kandungan mineral pasir dan liatnya.
Keyword: 

Judul: Daya dukung lahan dan faktor-faktor yang mempengaruhinya di Kabupaten Cianjur dan Karawang, Jawa Barat
Abstrak: Tujuan penelitian ini adalah untuk mengetahui besamya daya dukung sumberdaya lahan dan faktor-faktor yang mempengaruhinya di dua wilayah kabupaten yang memiliki keadaan lingkungan yang berbeda, terutama dari keadaan lingkungan fisik alamnya. Hasil dari penelitian ini diharapkan dapat berguna untuk mengetahui tingkat pedaya gunaan lahan di suatu wilayah; sebagai bahan pertimbangan bagi pemerintah daerah setempat dalam usaha meningkatkan produksi lahan pertanian tanaman pangan; untuk menentukan kebutuhan lahan per kapita dalam rangka memenuhi kebutuhan hidupnya, terutama kebutuhan akan pangan; dan sebagai bahan pertimbangan pemerintah daerah setempat untuk menentukan pilihan kegiatan pembangunan yang diperlukan di dalarn wilayahnya. Penelitian tela dilakukan di dua wilayah kabupaten. Kabupaten pertama adalah Kabupaten Cianjur yang merupakari wilayah daerah dataran tinggi yang mempunyai bentuk permukaan wilayah mulai agak datar, bergelamang, sampai bergunung, dengan mayoritas penggunaan lahannya berupa pertanian lahan kering (tegalan dan kebun campuran). Kabupaten kedua adalah Kabupaten Karawang yang merupakan wilayah dataran rendah dengan bentuk permukaan, wilayah sebagian besar datar dan mayoritas penggunaan lahannya berupa persawahan yang intensif.
Keyword: 

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable