Judul: Growth and Methane Oxidation Activity of Methanotrophic Bacteria in Different Media
Abstrak: Methanotrophs are bacteria that can utilize methane or C1 compounds as their sole carbon and energy source. Previous study resulted that BGM 1, BGM 3, BGM 9, and SKM 14 isolates could oxidized CH4. Objective of this research was to formulate medium composition for production of methanotrophic bacteria. The substances that we used as carbon sources were methanol, mollase. And a nitrate was as a nitrogen source. Specific growth rate of bacteria in methanol and nitrate was different for each isolate. The best carbon and nitrogen sources of medium for bacterial growth was methanol 2% and nitrate 1 g/L. Methane oxidation rate of BGM 1, BGM 3, BGM 9, and SKM 14 isolates was 0.01, 0.04, 0.03, and 0.03 mol/ml culture/day respectively. The highest activity of methane oxidation was perfomed by BGM 3 isolate, and the highest ammonium accumulation was perfomed by BGM 9 isolate. The bacterial cells produced by medium of combination methanol 2% and nitrate 1 g/L were tested in soil of a rice field in laboratorium scale. The result showed that methane concentration was decreased in twelveth days of incubation. The methane reduction activity of the isolates during incubation of 8 to 12 days was 88.5%, 85.1%, 47.8%, and 66.4% for BGM 1, BGM 3, BGM 9, and SKM 14 isolates respectively.
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Judul: Aktivitas Oksidasi Metan dan Reduksi Dinitrogen Oksida (N2O) Kultur Kombinasi Bakteri Metanotrof dan Ochrobactrum anthropi
Abstrak: Methane Oxidation and Nitrous Oxide (N2O) Reduction Activity on Combination Culture of Methanotrophic Bacteria and Ochrobactrum anthropi. Under supervision of IMAN RUSMANA and ALINA AKHDIYA. Rice fields are one source of methane (CH4) and nitrous oxide (N2O) emission. Methanotrophic bacteria and denitrifying bacteria possesing high methane oxidation and N2O reduction activity are very important for controlling CH4 and N2O emission from rice fields. This research was conducted to test the activity of methane oxidation and N2O reduction from four methanotrophic bacteria (BGM 1, BGM 5, BGM 9, and SKM 14 isolates) and two Ochrobactrum anthropi isolates (BL 1 and BL 2 isolates). Six combination were inoculated into Nitrate Mineral Salt (NMS) media mixed with denitrification media, then the activity of methane oxidation, N2O reduction, accumulation of ammonium, nitrite, and nitrate was analyzed. Combination culture E of BGM 1, SKM 14, BL 1, and BL 2 isolates had the highest CH4 oxidation (2,9 x 10-8 ìM/cell/day), N2O reduction activities (0,15 x 10-9 ìM/cell/day), and accumulation of ammonium (19,3 x 10-8 ìM/cell/day) than the other combination cultures. Combination culture of BGM 1, SKM 14, and BL 1 isolates had the highest accumulation of nitrate (17,6 ìM) and combination culture of BGM 5, BGM 9, BL 1, and BL 2 isolates had the highest accumulation of nitrite (10,9 ìM).
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Judul: Perencanan Lanskap Sunda Kalapa, Jakarta Utara Sebagai Kawasan W isata Sejarah
Abstrak: Perkembangan kota Jakarta dari waktu ke waktu sangatlah cepat, sehingga menyebabkan kota-kota tua banyak yang tergusur untuk dijadikan daerah baru yang lebih modern. Apabila hal tersebut tidak ditanggulangi secara dini maka keberadaan Kota Lama Jakarta akan hilang. Hal inilah yang melatar belakang untuk dilakukan tindakan perlindungan dan revitalisasi kawasan bersejarah Sunda Kalapa.
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