Judul: Konsep Taman Rumah Tinggal Tradisional Sunda Masyarakat Adat Rancakalong Sumedang Berbasis Budaya Lokal
Abstrak: Masyarakat adat Rancakalong adalah salah satu masyarakat tradisional di Indonesia yang memiliki cara sendiri untuk memanfaatkan dan melestarikan nilai-nilai adat dan budaya. Hal ini secara tidak langsung dapat mempengaruhi pola lanskap sekitarnya, termasuk taman rumah dan permukiman. Oleh karena itu, pelestarian diperlukan untuk mengantisipasi perubahan budaya. Hal ini dapat diterapkan dengan bentuk implementasi pelestarian kawasan melalui penetapan peraturan daerah, dan kegiatan riset terkait produk budaya. Tujuan dari penelitian ini adalah untuk mengindentifikasi dan menganalisis elemen-elemen taman dan tata letaknya pada taman rumah tinggal Rancakalong dan memformulasikan konsep taman rumah dari sudut pandang budaya masyarakat setempat. Taman rumah tradisional Rancakalong dibagi menjadi tiga bagian, yaitu halaman depan (buruan), halaman samping (pipir), dan halaman belakang (kebon). Elemen hardscape terdiri dari leuit, lisung, kolam (balong), kandang, kayu bakar dan paranggong. Sementara itu, Elemen softscape terdiri dari tanaman yang digunakan dalam sehari-hari dan upacara tradisional. Penempatan elemen taman tidak secara khusus ditentukan oleh aturan adat Rancakalong. Konsep taman rumah tradisional Rancakalong memiliki pola informal yang mencakup elemen hardscape dan softscape yang sesuai dengan budaya dan kebiasaan masyarakat adat Rancakalong
Keyword: lanskap budaya, pelestarian lanskap, desain taman, taman vernakular, permukiman tradisional, Eugenia

Judul: Kajian Desain Taman Rumah Tinggal Tradisional Budaya Sunda
Abstrak: Sundanese culture is one of the oldest culture in Indonesia. The study of traditional Sundanese garden needs to be done in order to get a real image about traditional Sundanese culture. The objectives of this research are to identify the elements, layout, and interpretation of sundanese garden. This research was obtained through descriptive analysis by field observation and tracing the information of culture and history. The study area are Urug Village, Bogor District; Sindang Barang Village, Bogor District; and Naga Village, Tasikmalaya District. The result of this study showed the spatial order of traditional Sundanese house divided into three parts in according the Tritangtu concept; those are the front or upper part, the central part, and back or under part. Yard of Sundanese house divided into three parts, those are the frontyard (buruan), sideyard (pipir), and the backyard (kebon). The dominant open space is an expanse of land or a grass. The forming elements of traditional sundanese garden are leuit, saung lisung, traditional bathroom (tampian), pond (balong), garden light (obor), cage of hen, and plants.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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