Judul: Komunitas cetacea dan karakteristik habitatnya di perairan Selat Ombai
Abstrak: As part of collaborative research INSTANT, this research aimed to explore spatio-temporal distribution of cetacean community observed in the Ombai Strait, as well as to study its biophysical characteristics, as the key to understanding cetacean habitat dynamics. During two research cruise in the Ombai Strait, December 2003-January 2004 and June-July 2005, cetacean community was assessed visually and resulted in consistent sightings of three apex predator cetacean species, e.g. Stenella longirostris, Pseudorca crassidens, and Physeter macrocephalus. The first two species were observed with large number of individuals, with foraging and feeding behavior. INSTANT Cruise I deployed 54 survey hours resulting in 5 positively identified cetacean species (Tursiops truncatus, Stenella longirostris, Pseudorca crassidens, Orcinus orca, Physeter macrocephalus), originated from 11 sightings, with estimated number of abundance 908 individuals; while INSTANT Cruise II resulted in 60 survey hours, 4 positive identification (Stenella longirostris, Pseudorca crassidens, Physeter macrocephalus, Kogia simus), 15 sightings, and 698 individuals. In general, 71.6% of cetacean sightings were at morning time (5:00-7:00 and 7:00-10:00 local time).
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Judul: Variabilitas suhu, salinitas, dan arus di Selat Ombai pada selang waktu September 2005 - November 2006
Abstrak: Selat Ombai merupakan salah satu jalur keluar massa air Arus Lintas Indonesia (ARLINDO), sehingga perairan ini memiliki peranan dalam sistem sirkulai massa air yaitu memasok massa air ke Samudera Hindia. Fenomena pergerakan massa air yang terjadi sepanjang tahun di perairan Indonesia akan mengakibatkan terjadinya perubahan parameter oseanografi baik suhu, salinitas, maupun arus. Penelitian ini bertujuan untuk mengkaji variabilitas suhu, salinitas, dan arus pada berbagai kedalaman 96 m-866m, serta mengkaji korelasi silang suhu, salinitas, arus dan angin di Selat Ombai. Data tersebut diperoleh dari hasil pengukuran mooring INSTANT di Ombai Selatan yang terletak pada koordinat 125° 02.262’ BT dan 08°32.335’ LS pada selang waktu September 2005 – November 2006 melalui situs (www.marine.csiro.au/~cow074/index.htm). Selain itu juga menggunakan data angin harian yang berasal dari badan riset NOAA-CDC pada koordinat 125°02.5’ BT dan 8°34’16.72” LS dengan selang waktu yang sama memiliki resolusi spasial 2,5 °Lintang dan 2,5 °Bujur. Pengolahan data dalam menentukan variabilitas temporal suhu dan salinitas dilakukan dengan menggunakan perangkat lunak MATLAB 7.0.1, sedangkan sebaran temporal angin dan arus dengan menggunakan Surfer 8.0. Sebaran temporal dipergunakan untuk menganalisis hubungan antara suhu, salinitas, arus dan angin. Spektrum densitas energi dihitung dengan metode FFT (Fast Fourier Transform) untuk melihat periode fluktuasi dari parameter suhu, salinitas, arus dan angin ditampilkan menggunakan perangkat lunak Statistica 6.0. Korelasi silang suhu, salinitas, arus, dan angin digunakan analisis Cross Spectral Analysis dengan bantuan perangkat lunak Statistica 6.0, untuk melihat hubungan antara suhu, salinitas, dan arus dengan angin, serta hubungan antara arus dengan suhu.
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Judul: Pengujian Virus Newcastle Disease Gen VII Isolat Lapang Sebagai Virus Standar Pada Uji Tantang Vaksin
Abstrak: This study was conducted to determine the standard of Newcastle disease virus Gene VII field isolate as a virus challenge to vaccine test. A virus suspension that has previously been tested by HA test then titrated with EID50 using 30 Specific Pathogen Free (SPF) of 10 day old chicken embryonated eggs. In addition, chicken lethal dose50 (CLD50) test were used to determine the virus titer that could cause 50% death of the chicken population. The CLD50 test was used 25 day-old-chickens (DOC) divided into 5 groups. The virus was diluted from 10-1 to 10-8. The 104 to 10-8 dilution of NDV was infected to every group of chicken via eyedrop and intranasal with 0.1 ml/chicken dosage. Haemagglutination test showed that the allantoic fluid were contain high titers of NDV is 512 HAU. The infective NDV that had been titrated by EID50 test showed the virus titers is 109 EID50/0.1 ml or 1010 EID50/ml and the virus titers of CLD50 test e.i 106.48 CLD50/0.1 ml or 107.48 CLD50/ml. These results can be concluded that the NDV Gene VII field isolates were most appropriate to be used as a standard virus to vaccine test
Keyword: Newcastle Disease Virus Gene VII, HA tes, EID50 test, CLD50 test