Judul: Keberadaan Beberapa Virus dan Efisiensi Tular Benih Squash mosaic virus pada Cucurbitaceae
Abstrak: Virus-virus pada tanaman Cucurbitaceae dapat menyebabkan kegagalan panen dan kerugian ekonomi yang tinggi. Salah satu virus yang terdapat pada Cucurbitaceae dan terbawa benih adalah Squash mosaic virus. SqMV berbahaya karena dapat menyebabkan kerugian yang sangat tinggi dan menjadi penghambat bagi negara penanam Cucurbitaceae seperti Indonesia. Pengujian keberadaan Cucumber mosaic virus (CMV), Squash mosaic virus (SqMV), Watermelon mosaic virus-2 (WMV-2), Zucchini yellow mosaic virus (ZYMV), dan Tobacco ringspot virus (TRSV) pada sampel daun dan benih Cucurbitaceae dilakukan dengan menggunakan metode Indirect-ELISA. Pengujian bobot molekul virus dilakukan menggunakan metode Western blot. Benih Cucurbitaceae berasal dari distributor benih dan dari tanaman sakit. Sampel Cucurbitaceae hasil survei terinfeksi secara tunggal atau ganda oleh CMV, SqMV atau ZYMV. Benih Cucurbitaceae yang berasal dari distributor benih terinfeksi oleh SqMV dan ZYMV. SqMV ditemukan pada 13.3% benih oyong dan semangka, 33.3% benih zucchini, 73.3% benih kabocha, dan 100% benih mentimun dan melon. ZYMV hanya ditemukan pada benih oyong dan zucchini berturut-turut 13.3% dan 26.67%. SqMV ditemukan pada biji mentimun, oyong, dan melon yang berasal dari benih tanaman sakit berturut-turut pada 93.3%, 100%, dan 100% benih. Bobot molekul SqMV 45 kDa dan 69 kDa. SqMV telah berada di Jawa Barat. Oleh karena itu status SqMV sebagai OPTK A1 golongan 1 perlu ditinjau kembali.
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Judul: Efisiensi Penularan Cucumber mosaic virus, Zucchini yellow mosaic virus, dan Squash mosaic virus Melalui Benih Cucurbitacea
Abstrak: Seed-transmitted viruses have been known to cause negative effect on seed germination and seedling growth and may cause disease spread more widely in the field. Research was conducted to identify seed-transmitted viruses from Cucurbitaceae and to study their potential role as primary inoculum in the field. Cucurbitaceae seeds used in the experiment i.e. cucumber, bottle gourd, watermelon, zucchini, melon, and kabocha were obtained from commercial seeds available in the market. Evaluation of seed-transmitted viruses was done using growing on test method. I-ELISA and DIBA was used for detection of virus infection. Three viruses i.e. CMV, SqMV, and ZYMV were detected from cucumber field in Situgede, Dramaga, Bogor. CMV was detected from melon variety Kinanti and zucchini seed (2%) as well as from kabocha seed (12%). SqMV was detected 80%, 6%, and 2% from cucumber, melon variety Kinanti and bottle gourd seeds, respectively. Virus infection was detected in seedling up to 5 weeks after transplanting, i.e. CMV in kabocha and SqMV in cucumber. This results indicated the potential role of seed-transmitted viruses as primary inoculums in the field
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Judul: Hydroxyapatite-Chitosan Composite Coating on Stainless Steel 316 to Improve Corrosion Resistance
Abstrak: Metals that were used in bone implantation should have good corrosion resistance that were not easily corroded when implanted into the body. Metal corrosion resistance of stainless steel 316 can be enhanced through the metal coating by hydroxyapatite-chitosan composite with composition ratio of hydroxyapatite (gram) and chitosan 3% (mL) 0.2:0, 0.2:0.1, 0.2:0.5, 0.2:1.0, and 0.2:1.5. Besides improving the corrosion resistance of metal, the presence of chitosan also maintain the position of hydroxyapatite on metal surfaces. Corrosion resistance increases with increasing amount of chitosan added and the best composition of hydroxyapatite-chitosan composite was 0.2:1.0.
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