Judul: Content Analysis of The Instagram Account @Dearmoms.id on Fulfulling Parenting Information Needs
Abstrak: Penggunaan media instagram sebagai media informasi ditandai dengan banyak ditemukannya akun-akun komunitas yang berfokus untuk membagikan informasi, salah satunya informasi parenting. Tujuan dari penelitian ini adalah menganalisis konten akun instagram @dearmoms.id dalam memenuhi kebutuhan informasi parenting dan bagaimana hubungannya dengan faktor individu dan penggunaan media sosial. Penelitian ini menggunakan pendekatan kuantitatif dan didukung dengan data kualitatif. Metode yang digunakan dalam penelitian ini adalah metode survei dan analisis isi konten akun instagram @dearmoms.id. Responden dan konten dipilih dengan menggunakan metode nonprobability sampling dengan teknik purposive sampling. Hasil penelitian menunjukkan tidak terdapat kesesuaian antara konten dengan pemenuhan kebutuhan informasi parenting. Faktor individu yang berhubungan nyata dengan pemenuhan kebutuhan informasi adalah status usia anak. Penggunaan media sosial yang menghasilkan atensi yang tinggi memiliki hubungan yang nyata dengan pemenuhan kebutuhan informasi parenting
Keyword: instagram, information needs, parenting

Judul: The Effectiveness of the Instagram Account @infosumbar as a Media for Disseminating Information on Minangkabau History and Culture
Abstrak: Instagram menjadi salah satu media sosial terpopuler yang digunakan oleh masyarakat saat ini. Peluang tersebut digunakan oleh PT Infosumbar Media Kreasi untuk memanfaatkan Instagram sebagai media dalam menyebarkan informasi sejarah dan budaya lokal di tengah warisan kebudayaan Indonesia. Penelitian ini menganalisis efektivitas akun Instagram @infosumbar sebagai media penyebaran informasi sejarah dan budaya Minangkabau. Penelitian ini menghubungkan variabel terpaan penggunaan Instagram dengan tingkat efektivitas Instagram yang dilihat dari efek pesan komunikasi terhadap aspek kognitif, afektif, dan konatif pengikutnya. Penelitian ini dilakukan dengan pendekatan kuantitatif dengan metode survei dan observasi sebagai instrumen pengambilan data kuantitatif yang didukung dengan data kualitatif dari wawancara mendalam. Data kuantitatif diolah menggunakan uji korelasi dengan pemilihan responden yang menggunakan metode purposive sampling. Hasil penelitian menunjukkan terdapat hubungan nyata antara tingkat terpaan pengikut akun Instagram @infosumbar dengan tingkat efektivitas akun Instagram @infosumbar dalam aspek kognitif, afektif, dan konatif., Instagram is one of the most used social media by people today. PT Infosumbar Media Kreasi took this opportunity to use Instagram as a medium in disseminating information on local history and culture in the midst of Indonesia's cultural heritage. This study analyzes the effectiveness of the Instagram account @infosumbar as a medium for disseminating information on Minangkabau history and culture. This study connects the variable exposure to Instagram use with the level of effectiveness of Instagram seen from the effect of communication messages on the cognitive, affective, and conative aspects of followers. This research was conducted with a quantitative approach with survey and observation methods as a quantitative data collection instrument supported by qualitative data from in-depth interviews. Quantitative data was processed using a correlation test with the selection of respondents using a purposive sampling method. The results showed that there was a significant relationship between the level of exposure of followers of the Instagram account @infosumbar and the level of effectiveness of the Instagram account @infosumbar on cognitive, affective, and conative aspects.
Keyword: effectiveness, instagram, social media exposure

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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