Judul: Analisis tingkat kepuasan pedengar terhadap PT Radio Ika Lesmana
Abstrak: PT. Radio Ika Lesmana merupakan salah satu perusahaan yang bergerak dalam bisnis penyiaran radio. Persaingan yang semakin ketat dalam bisnis penyiaran radio, mewajibkan PT Radio Ika LEsmana agar menempatkan kepuasan pendengar sebagai prioritas utama.
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Judul: The Level of Listener Satisfaction in the Broadcast Services of Programa 1 RRI Bogor
Abstrak: Radio sebagai media massa konvensional perlu beradaptasi terhadap perkembangan zaman agar mampu bersaing dengan media baru demi terus menjaga eksistensinya. Stasiun radio sebagai penyedia layanan bertanggung jawab untuk senantiasa menyempurnakan kualitas siarannya agar mampu memenuhi pelayanan yang diharapkan pendengar. Tujuan dari penelitian ini untuk menganalisis kualitas layanan siaran yang penting bagi Pro 1 RRI Bogor sehingga dihasilkan strategi yang tepat untuk meningkatkan kepuasan pendengar. Penelitian ini merupakan penelitian deskriptif menggunakan pendekatan kuantitatif dengan menyebarkan kuesioner online kepada 45 responden serta didukung oleh data kualitatif melalui wawancara mendalam terhadap informan. Teknik pemilihan sampel yang digunakan adalah snowball sampling. Hasil penelitian menunjukkan kualitas layanan siaran yang diberikan Pro 1 RRI Bogor sesuai dengan harapan pendengar. Tingkat kepuasan pendengar berdasarkan perhitungan Customer Satisfaction Index bernilai 86,44 persen yakni bermakna Sangat Puas. Strategi peningkatan kualitas siaran dirumuskan dengan memprioritaskan atribut layanan yang berada pada Kuadran I dalam matriks Importance-Performance Analysis yaitu (1) penyelenggaraan acara off-air, (2) segmen interaktif, dan (3) segmen hiburan., Radio as a conventional mass media needs to adapt to the evolving times to compete with new media and maintain its existence. Radio stations as service providers are responsible for continually improving their broadcast quality to meet listener expectations. This study aims to analyze the broadcast service quality crucial to Pro 1 RRI Bogor and develop appropriate strategies to enhance listener satisfaction. This descriptive research employs a quantitative approach by distributing online questionnaires to 45 respondents supported by qualitative data from in-depth interviews with informants. The sampling technique used is snowball sampling. The results show that the broadcast service quality of Pro 1 RRI Bogor meets listener expectations. The listener satisfaction level based on the Customer Satisfaction Index is 86,44 percent, indicating Very Satisfied. Strategies for improving broadcast quality are formulated by prioritizing service attributes in Quadrant I of the Importance-Performance Analysis matrix, namely (1) off-air events, (2) interactive segments, and (3) entertainment segments.
Keyword: broadcast service quality, listener satisfaction, CSI, konstipasi

Judul: Gene Editing  of Two Cyanide Synthesis-related Genes in Cassava using CRISPR/Cas9
Abstrak: Cassava (Manihot esculenta) contain toxic quantities of cyanogenic glucoside. Reducing the potentially toxic cyanogenic glucoside levels in cassava could be achieved by gene editing through Clustered Regularly Interspaced Short Palindromic Repeats – CRISPR associated protein 9 (CRISPR/Cas9), a high- precision genome editing technique in plants. Two cassava UDP- glucosyltransferase (UGT) paralogs, UGT85K4 and UGT85K5, are the genes catalyzing the synthesis of cyanogenic glucosides in plants. The study aimed to construct the CRISPR/Cas9 recombinant vector carrying single guide RNA (sgRNA) to edit UGT85K4 and UGT85K5 genes, and transform sgRNA CRISPR/Cas9 construct into KU50 cassava calli. Five sgRNAs were designed, considering the on/off target efficiency score and GC content. Those sgRNAs were ligated into pRGEB32 plasmid and introduced to competent Escherichia coli DH5a. The PCR colony and DNA sequencing were performed to verify the sgRNA insert. The amplicons showed the expected band size of recombinant vector (180 bp). Based on sequencing results, only one sgRNA was successfully ligated into the plasmid that will be used to edit the UGT85K5 gene. The sgRNA CRISPR/Cas9 construct in pRGEB32 plasmid was successfully transformed into Agrobacterium tumefaciens and introduced into KU50 cassava calli through co-cultivation. The overgrowth of Agrobacterium occurred after the co-cultivation phase.
Keyword: CRISPR/Cas9, Cyanogenic glucoside, Gene editing, sgRNA