Judul: Pengetahuan dan Sikap Gizi Ibu dengan Praktek Konsumsi Susu Anak Sekolah TK
Abstrak: Thisstudy is aimed to analyze correlation between mother’s nutritional knowledge and attitude and milk consumption of pre-school children’s (PSC). A cross sectional study design was applied among 136 PSC aged 4-5 yrs old in the two favorite kindergartens of Bogor City - Aliya and Mexindo. The mothers f the PSC filled in a questionnaire consist of sociodemographic characteristics, children’s milk consumption, and a nutritional knowledge-and-attitude. Milk consumsption of PSC was measured by applying a 30 days semi-FFQ method . The results show that most of mother had good nutritional knowledge and attitiude. More than 90% of them agree that drinking milk is a good habit for children. The mother’s nutritional attitudes showed significant positive correlation with children’s milk drinking habit (r = 0.31). No relation was found between nutritional knowledge with attitude and milk consumption
Keyword: pre-school children., milk consumption, nutritional attitude, nutritional knowledge

Judul: Hubungan Konsumsi Susu dengan Tinggi Badan Anak Sekolah TK
Abstrak: The objective of this study was to analyze the correlation between milk consumption and body height of pre-school children (PSC). The study was conducted by applying a cross sectional study design implemented among 136 PSC aged 4-5 yrs old in the two favorite kindergartens of Bogor City - Aliya and Mexindo. Milk intake was derived from the intake data collected by applying a 30 days semi-FFQ method filled in by the mothers. The body height of PSC is direcly measured by the researchers. The statistical analysis showed that there are positive significant correlation between body height and milk intake frequency, amount of milk intake, animal protein intake, total protein intake, and calcium intake from milk respectively (p<0.05), but there is no correlation between body height and calcium intake from non-milk (p>0.05). Children who consumed milk every day are taller than children who are not consumed (p<0.05).
Keyword: preschool children., body heigh, milk intake, milk calcium intake

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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