Judul: Analisis kandungan klorofil dan laju fotosintesis tebu transgenik PS-IPB 1 yang di tanam di kebun percobaan PG Djatiroro, Jawa Timur
Abstrak: Sugar cane (Saccharum officinarum Linn.) is an important industrial plant for sugar production. The productivity of this plant could be increased by introducing phytase gen. Such transgenic sugarcane that had been produced by Santosa et al. (2004), is expected to increase the efficiency of P fertilization. The objectives of this research were (1) to select best growth performance of transgenic sugar cane clones treated with P fertilization, (2) to measure of chlorophyll content and Leaf Area Index (LAI) of the selected transgenic sugar cane. Plantation of the transgenic sugar cane was conducted at the experimental station of PG Djatiroto, PTPN XI, East Java. Plantation was treated with two levels of P fertilization , i.e. 25% and 50% of optimum P fertlization (P2O5 = 92,29 ppm). Growth performance parameters of the plant that were measured including leave sizes, number of space between joints, number or stalk, stalk height, and stalk diameter. Samples of leaves for analysis of chlorophyll and LAI were collected at 3 and 6 months after plantation. The result show that not all of sugar cane transgenic clones (PS-IPB 1) has chlorophyll higher than its isogenic (PS-851). At 3 months old the clones contained chlorophyll-a higher than the isogenic are 6 clones in the test field I, and 1 clone in the test field II; while that for chlorophyll-b are 9 clones in the test field I, and 3 clones in the test field II. At 6 months old the clones contained chlorophyll-a higher than the isogenic are 10 clones the test field I, and 5 clones in the test field II; while that for chlorophyll-b are 9 clones in the test field I, and 6 clones in the test field II. In average, chlorophyll-a, chlorophyll-b, and total chlorophyll of the plants grown in the field I are higher than that in the field II. In addition, chlorophyll-b of the transgenic plant in both fields is higher than chlorophyll-a. There is no differences of Leaf Area Index (LAI) of plants grown in the field I and II, but the LAI of plants at age 6 months is significantly higher than that at age 3 months.
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Judul: Analisis kandungan unsur N dan P tebu transgenik PS-IPB 1 yang mengekspresikan gen fitase
Abstrak: Formerly Indonesia is one of the biggest sugar exporter country in the world, nowdays Indonesia became one of the biggest sugar importer countries. There are several causes of low sugar production in Indonesia, one of them is low productivity due to inefficiency of fertilizer use, for example P fertilizer. It’s though that the isertion of phytase gene to the sugarcane genome will increase the efficiency of P fertilizer use and P metabolism in plant. The purpose of the study were 1) to test fitase gen expression in sugar cane transgenic PS-IPB 1 clones and select the five best clones 2) to select clones that have probable high rendemen. The sugarcane plant were planted in two different treatment of fertilizing, which are 25% Phosphor from recommendation in test site 1 and 50% Phosphor from recommendation in test site 2. Analysis are done in vegetatif stage, which in age 3 and 6 months. For Nitrogen and Phosphor analysis, 20 clones of the best sugar cane transgenic growing quality were used based on many varieties that undergoing selection with scoring frequency spread data. Analysis were done using sample of the lowest leaf from sugar cane transgenic clones. Based on the analysis, not all clones of sugar cane transgenic PS IPB 1 had Nitrogen and Phosphor content more higher than isogenic PS 851. Nitrogen and Phosphor content of sugar cane transgenic and the isogenic at the age of 3 months relatively more higher than at the age of 6 months, so does from the test site 2 (50% Phosphor treatment) is higher than sugar cane transgenic from test site 1 (25% Phosphor treatment). Based on selection with scoring frequency spread data, there are 5 sugar cane transgenic clones that have the highest rendemen (sucrose content of sugar cane) such as PS IPB 1-59, PS IPB 1-20, PS IPB 1-55, PS IPB 1- 5, and PS IPB 1-53. While there are some sugar cane transgenic clones that exceed in any criteria such as PS IPB 1-55, PS IPB 1-5, PS IPB 1-20, PS IPB 1- 59, PS IPB 1-29.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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