Judul: Business Model Design and Financial Analysis for Sweet Premix Product at PT LeGitt Boga Mandiri, Bogor
Abstrak: PT LeGitt Boga Mandiri sebagai perusahaan makanan berbasis tepung terigu sedang mengembangkan produk baru yaitu tepung sweet premix corn dog. Agar proses pengembangannya berjalan dengan lancar, dibutuhkan perancangan model bisnis yang tepat dan juga analisis finansial guna mengetahui apakah produk tersebut tepat dan layak untuk diluncurkan oleh PT LeGitt Boga Mandiri. Perancangan model bisnis dilakukan dengan pendekatan Lean Canvas. Analisis finansial dilakukan dengan metode discounted cashflow. Harga Pokok Produksi dari produk ini adalah Rp. 22.513/Kg dan hasil dari perhitungan analisis finansial didapat: (1) Nilai NPV Rp. 106.604.854, (2) IRR 58%. Dari segi kelayakan, produk ini layak untuk diteruskan oleh PT LeGitt Boga Mandiri karena setelah ditinjau dari aspek finansial maupun non finansial, produk ini layak dan tidak sensitif terhadap perubahan harga bahan baku utama., PT LeGitt Boga Mandiri, a flour-based food manufacturing company, is currently developing a new product. The product is a premix flour for making corn dog. To ensure a smooth development process requires a proper business model design and financial analysis to determine whether the product is suitable and feasible for launch by PT LeGitt Boga Mandiri. The business model is designed using Lean Canvas, and the financial analysis is conducted using discounted cashflow method. The Total Manufacturing Cost (TMC) for this product is Rp.22.513/Kg, and the results of the financial analysis are as follows: (1) NPV value of Rp.106.604.854, (2) IRR of 58%. From a feasibility perspective, this product is suitable to be adopted by PT LeGitt Boga Mandiri because, after considering both financial and non-financial aspects, the product is feasible and insensitive to changes in main raw material prices.
Keyword: Business Model, Financial Analysis, Lean Canvas, Net Present Value (NPV), Product Development

Judul: Scale-up and Standard Operating Procedures for Sweet Premix Production at PT LeGitt Boga Mandiri
Abstrak: PT LeGitt Boga Mandiri merupakan usaha perseroan terbatas dalam bidang food and beverage yang memproduksi roti burger, roti sandroll, dan tepung panko (fresh breadcrumb). Perusahaan telah mengembangkan tepung savoury premix untuk tepung salut ayam dan udang. Perusahaan berencana untuk memperluas bisnisnya dengan mengembangkan produk tepung sweet premix. Agar proses yang dikembangkan di laboratorium dapat dijalankan dalam skala besar, maka diperlukan Standard Operating Procedure (SOP) sebagai pedoman serta penggandaan skala produksi. Berdasarkan tahap identifikasi proses, pembuatan sweet premix terdiri dari tahap pengayakan, penimbangan, pencampuran, pengemasan, dan penyimpanan sementara. Proses tersebut dilakukan menggunakan mesin vibrating screen separator (pengayak), planetary mixer, horizontal ribbon mixer, mesin jahit, dan metal detector. Hasil perhitungan neraca massa menunjukkan bahwa loss produksi yang dihasilkan 0,96%. Hasil dari penelitian ini adalah standarisasi proses dalam dokumen SOP serta rancangan penggunaan mesin dan peralatan., PT LeGitt Boga Mandiri is a limited liability company in the food and beverage sector that produces burger buns, sandroll buns, and panko flour (fresh breadcrumb). The company has developed savoury premix flour for chicken and shrimp salute flour. The company plans to expand its business by developing sweet premix flour products. In order for the process developed in the laboratory to be carried out on a large scale, a Standard Operating Procedure (SOP) is needed as a guideline as well as multiplication of production scale. Based on the process identification stage, the manufacture of sweet premix consists of sieving, weighing, mixing, packaging, and temporary storage. The process is carried out using a vibrating screen separator, planetary mixer, horizontal ribbon mixer, sewing machine, and metal detector. The results of the mass balance calculation show that the resulting production loss is 0.96%. The result of this research is the standardization of the process in the SOP document as well as the design of the use of machinery and equipment.
Keyword: scale up, SOP, sweet premix

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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