Judul: Pengaruh Karakteristik Sosial, Demografi dan Ekonomi terhadap Keberdayaan Konsumen pada Pembelian Online.
Abstrak: Internet telah menjadi sarana yang umum digunakan untuk proses kegiatan penjualan dan pembelian. Pertumbuhan internet sebagai tempat transaksi penjualan dan pembelian tidak diimbangi dengan payung hukum yang jelas. Konsumen saat ini lebih rentan terjebak dalam penipuan sehingga pemberdayaan konsumen sangat penting untuk dilakukan. Penelitian ini bertujuan untuk menganalisis pengaruh karakteristik sosial, demografi dan ekonomi terhadap keberdayaan konsumen pada pembelian online. Penelitian ini menggunakan desain cross-sectional study terhadap 100 orang yang melakukan pembelian online menggunakan online survey. Analisis data menggunakan analisis deskriptif dan inferensia. Hasil penelitian dapat disimpulkan bahwa pada pembelian online responden laki-laki, berusia dewasa awal (19-24 tahun), berpendidikan tinggi (>12 tahun), bertempat tinggal di perkotaan, suku Jawa, berpendapatan <1 000 000 per bulan dan bekerja adalah responden yang paling berdaya dibandingkan kelompok responden lainnya. Uji regresi linier berganda menunjukkan bahwa jenis kelamin laki-laki, suku selain Sunda dan pendapatan yang rendah cenderung akan meningkatkan nilai indeks keberdayaan konsumen.
Keyword: Karakteristik sosio demografi, keberdayaan konsumen, pembelian online

Judul: Pengaruh Karakteristik Demografi, Sosial dan Ekonomi terhadap Keberdayaan Konsumen Produk Elektronik.
Abstrak: Kebutuhan manusia sebagai konsumen khususnya rumah tangga tidak terlepas dari berbagai macam kebutuhan hidup. Rendahnya kesadaran konsumen tentang haknya memicu kurangnya kemampuan konsumen melindungi diri dari pelaku usaha yang curang. Penelitian ini bertujuan untuk menganalisis pengaruh karakteristik demografi, sosial dan ekonomi terhadap keberdayaan konsumen pada produk elektronik. Penelitian menggunakan desain cross sectional terhadap 100 responden yang melakukan pembelian produk elektronik dengan online survei. Analisis data menggunakan analisis deskriptif dan inferensia. Analisis deskriptif digunakan untuk mengidentifikasi karakteristik demografi, sosial dan ekonomi responden dan indeks keberdayaan konsumen. Analisis inferensia yang digunakan adalah uji chi-square dan regresi logistik. Hasil dari penelitian ini menunjukkan bahwa responden perempuan, kategori usia dewasa awal (18-40 tahun), tingkat pendidikan tinggi, bekerja, berpendapatan Rp2 000 000 – Rp3 000 000 per kapita per bulan, tinggal di perkotaan dan jumlah anggota keluarga ≤ 4 orang lebih berdaya dibandingkan kategori responden lainnya. Tingkat pendidikan berpengaruh positif signifikan terhadap keberdayaan konsumen pada produk elektronik.
Keyword: Keberdayaan konsumen, karakteristik sosio demografi, produk elektronik

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable