Judul: Hubungan antara Pengetahuan dan Sikap Ibu tentang Gizi dan Posyandu dengan Status Gizi Balita di Kecamatan Pasar Rebo
Abstrak: Knowledge and mother’s attitudes about nutrition and posyandu are required to improve children feeding pattern so that adequate nutrition for the children is reached, and in this way they can grow and develop well. The purpose of this study was to analyze the correlation between knowledge and mother’s attitudes about nutrition and posyandu with nutritional status of children in subdistrict Pasar Rebo. Study design used in this study was a cross-sectional design. The sampling of this reasearch was 100 mother and their underfive children. Data processing and analysis was done using Microsoft Excel and the Statistical Package for Social Science (SPSS) version 16.0 for Windows. Based on the results of statistical test, there was a significant correlation between the level of participation of mothers with nutrition knowledge levels (p<0.05). The level of mothers nutrition knowledge had no significant correlation to level of energy and protein adequacy (p> 0.05). Mothers nutrition knowledge level had no significant correlation with nutritional status of children based index weight for age, height for age, and weight for height (p> 0.05).
Keyword: posyandu., nutritional status, nutrition attitudes, Nutrition knowledge

Judul: Hubungan Pola Asuh Dan Pengetahuan Gizi Ibu Dengan Status Gizi Dan Kesehatan Anak Balita
Abstrak: Penelitian ini bertujuan untuk menganalisis hubungan antara pola asuh dan pengetahuan gizi ibu dengan status gizi dan kesehatan anak balita dengan desain penelitian cross sectional study. Jumlah sampel dalam penelitian ini 50 orang. Hasil penelitian menunjukkan bahwa 58% ibu telah memiliki pengetahuan gizi yang cukup baik dan menerapkan pola asuh makan yang baik serta 70% pada pola asuh kesehatan dengan kategori baik. Status gizi sampel sebagian besar telah termasuk ke dalam kategori baik (BB/TB) (92%), (TB/U) (90%), dan (BB/U) (92%). Sebanyak 80% sampel memiliki skor morbiditas yang rendah. Terdapat hubungan yang signifikan (p<0,05) antara pengetahuan gizi dengan pola asuh (makan dan kesehatan). Terdapat hubungan yang signifikan (p<0,05) antara status gizi (BB/TB dan BB/U) dengan tingkat konsumsi energi. Terdapat hubungan (p<0,05) antara tingkat asupan protein dengan status gizi.
Keyword: Balita, Pola Asuh, Pengetahuan Gizi, Status Gizi

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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