Judul: Kebiasaan Makan Pagi dan Jajan serta Status Gizi Anak Sekolah Dasar di Kota Bogor
Abstrak: Tujuau umum penelitian ini adalah untuk mengetahui kebiasaan makan pagi dan jajan serta status gizi anak sekolah dasar (SD). Tujuan khususnya adalah untuk: (1) meugetahui kebiasaau makan pagi,jajan. dan status gizi anak SD dan (2) mengetahui hubungan antara kebiasaan makan pagi dan jajan denan status gizi anak SD.
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Judul: Kebiasaan jajan, aktifitas fisik, status gizi dan kesehatan serta hubungannya dengan prestasi belajar siswa Sekolah Dasar di Kota Bogor
Abstrak: Secara umum penelitian ini bertujuan untuk mempelajari kebiasaan jajan, aktivitas fisik, status gizi dan kesehatan serta hubungannya dengan prestasi belajar siswa Sekolah Dasar di Kota Bogor. Tujuan khusus dari penelitian ini adalah 1) menganalisis karakteristik siswa laki-laki dan perempuan, 2) menganalisis kebiasaan jajan siswa laki-laki dan perempuan, 3) menganalisis konsumsi pangan siswa laki-laki dan perempuan, 4) menganalisis aktivitas fisik siswa laki-laki dan perempuan, 5) menganalisis status kesehatan siswa laki-laki dan perempuan, 6) menganalisis status gizi siswa laki-laki dan perempuan, 7) menganalisis prestasi belajar siswa laki-laki dan perempuan, 8) menganalisis hubungan konsumsi pangan, aktifitas fisik dan status kesehatan dengan status gizi siswa, 9) menganalisis hubungan prestasi belajar dengan status gizi dan kesehatan siswa
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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