Judul: De Novo Assembly of Sengon (Falcataria moluccana Miq.) Reference Genome
Abstrak: Sengon (Falcataria moluccana Miq.) merupakan komoditas unggulan hutan rakyat yang berpotensi dikembangkan menjadi jenis unggulan industri perkayuan. Serangan boktor (Xystrocera festiva) menjadi ancaman besar yang dapat menurunkan produksi hingga 100%. Pengendalian molekuler yang didukung perkembangan teknologi dapat digunakan untuk mengetahui gen-gen resisten yang mendukung program pemuliaan sengon. Penelitian ini bertujuan menyusun sekuens genom referensi sengon secara de novo dan mengidentifikasi keberadaan gen Alpha-amylase inhibitor (AI) dan Trypsin inhibitor (TI) pada sekuens genom yang disusun. Penyusunan genom dilakukan dengan sequencing DNA, pengecekan kualitas reads data, genom assembly, dan anotasi genom. Ray assembler memiliki cakupan genom yang terbaik walaupun sebgaian besar gen BUSCOnya tidak lengkap diterjemahkan. Terdapat dua sekuens yang mengandung gen Alpha-amylase Inhibitor (AI) dan 19 sekuens mengandung gen Trypsin Inhibitor (TI)., Sengon (Falcataria moluccana Miq.) is a leading commodity for community forests and potential to be developed into a superior type on timber industry. The presence of boktor (Xystrocera festiva) is major threat to this commodity that can reduce production up to 100%. Molecular control supported by technological developments can be used to determine resistance genes to support breeding program. This study aims to compile de novo reference genome of sengon and identify the presence of Alpha-amylase inhibitor (AI) and Trypsin inhibitor (TI) genes in the compiled genome sequence. The genome assembly methods by DNA sequencing, quality control of reads data, genome assembly, and genome annotation. Ray assembler has the best genome coverage although the completeness of the BUSCOs gene is widely classified as missing gene. There were two sequences containing the Alpha-amylase Inhibitor (AI) gene and 19 sequences containing the Trypsin Inhibitor (TI) gene.
Keyword: alpha-amylase inhibitor, Falcataria moluccana, next generation sequencing, trypsin inhibitor, whole genome sequencing

Judul: Analisis Transkriptomik Pohon Sengon (Falcataria moluccana Miq.) Rentan dan Resisten terhadap Hama Boktor (Xystrocera festiva Pascoe).
Abstrak: Sengon (Falcataria moluccana Miq.) plantations in monoculture system has increases boktor (Xystocera festiva Pascoe) pest population and distribution, however there is no effective and efficient control method yet. This study aims to analyze sengon transcriptomic data and identify expressed genes involved in sengon resistance to boktor pests, also other genes related to abiotic and biotic stresses. Unpublishe de novo transcriptomic data was acquired using the RNAseq and deposited in the DDBJ repository with accession number DRA008389. DEG analysis was perfomed using R program with edgeR package. The results of gene screening were then annotated using genes analog with the Swissprot database. DEG analysis produced 6859 upregulated contigs, genes in resistant tree were Heat shock cognate 70 kDA protein, Trypsin inhibitor, and Chalcone synthase. Based on previous research, trypsin inhibitor is a defensive mechanism of sengon against boktor pests, while other genes are known to be involved in the abiotic and biotic stress response.
Keyword: de novo transcriptomic, Falcataria moluccana Miq, trypsin inhibitor, Xystocera festiva Pascoe

Judul: Metrik Einstein (Anti-) Self-Dual Berdimensi Empat dengan Metode Cartan
Abstrak: Gravitational field equation can be formulated in differential forms (differential geometry), which describes gravity as a manifestation of the curvature of space-time. Curvature of space-time is characterized by a metric that satisfies the gravitational field equation. With differential forms it can be formulated the (anti-) self-dual Einstein equation, which is a modification of the Cartan structure in (anti-) self-dual basis. Calculation of (anti-) self-dual metric with (anti-) self-dual Einstein equation is simpler than the calculation using Christoffel symbols.
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