Judul: Optimasi Fonnula Media Pertumbuhan Isolat Bakteri SCG 1223 Penghasil Bakteriosin Berbasis Ekstrak Tauge.
Abstrak: Bakteriosin merupakan metabolit mikroba bakteri asam laktat (BAL) yang dapat menghambat pertumbuhan mikroba lain. Penggunaan bakteriosin sebagai II biopreservatif berperan untuk meningkatkan keamanan dan daya simpan pangan. ~ Proses produksi bakteriosin yang efisien dan efektif diperoleh dengan cara :I: mengoptimasikan faktor-faktor produksi yaitu jenis bakteri prod user, kondisi fisik III ;II:' fermentasi dan nutrisi media tumbuhnya. ~ Media yang biasa digunakan untuk memproduksi bakteriosin adalah media iii komersial seperti MRS (de Man Rogosa Sharpe) dan TGE (Tryptone Glucose 3 Extract). Barga media terse but relatif mahal dan komposisi nutrien lebih banyak ~ dibandingkan dengan yang dibutuhkan oleh mikroba untuk memproduksi "'C bakteriosin. Untuk itu periu disusun media produksi yang harganya Iebih murah, OJ sesuai kebutuhan nutrisi mikroba produser serta mudah diperoleh. Sumber N ::l daJam media produksi berpengaruh terhadap efektivitas produksi bakteriosin. III ~ Kecambah kacang hijau memiliki kandungan protein yang dapat digunakan S. sebagai sumber N dalam fennentasi bakteri asam laktat untuk menghasilkan ~ senyawa protein bakteriosin. ;- Penelitian ini bertujuan untuk mengetahui kemampuan bakteriosin dari ~ BAL SCG 1223 yang ditumbuhkan pada media ekstrak tauge dalam menghambat ::l Escherichia coli, Salmonella thypimurium dan Listeria monocytogenes, ~ mendapatkan persamaan matematis yang menggambarkan pengaruh konsentrasi cg dari fonnulasi media produksi ekstrak tauge yang diperkaya ekstrak khamir dan .::!.. pepton serta mendapat titik optimum konsentrasi komponen media produksi bakteriosin.
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Judul: Produksi Bakteriosin dari Bakteri Asam Laktat Galur SCG 1223 dalam Media Molasses
Abstrak: Bacteriocins are proteinaceous (generally peptide) which can inhibit the growth bactery pathogen that similar or closely related bacterial strain with the cell producer of bacteriocins. Efficiency and effectivitiy of bacteriocins production can be obtained by optimizing production factors. Bacteriocins given much benefit for industrial food, because bacteriocins can reduced the contaminant microorganism causing food spoiled and food borne illness diseas. Usually media for bacteriocins production is MRS Broth (de Mann Rogosa Sharpe). But MRS broth is expensive for industrial scale production. Because of that, we need to find alternative media which is cheaper but effective for growth medium. Molasses is one of ingredient that usually to use as medium growth of microorganism. To optimized the growth of lactic acid bacteria (LAB), molasses media can be added by yeast extract, peptone, and tween 80. This research obtain to know ability bacteriocins from LAB SCG 1223 which growth in molasses medium and get formula medium based on melasses media that is efficient to produce bacteriocins and effective to inhibit the bacterial pathogen. Indicator bacterial was needed to know the ability of bacteriocins to inhibit the growth bactery pathogen. The more bactery pathogen species which can inhibit by bactericins more wide the spectrum inhibit activity. The inhibit activity of bacteriocins was known by the transparent zone around the colony. According to the result of this research, formula media M4K1P2 which consist of molasses 4%, yeast extract 1%, peptone 2%, and tween 80 1% is effective to inhibit pathogen bacterial. The formula M4K1P2 has inhibit activity 1004.80 AU.ml against E. Coli, 2047.59 AU/ml against L. Monocytogenes, and 529.80 AU/ml against S. Thypimurium. Inhibit activity is an ability for one molecul bacteiocin to inhibit single cell pathogen microorganism which we can assume from the wide of the clear zone.
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Judul: Characteristic of Overshooting Top (OT) Based on  Himawari  8  Satellite  as  a  Convective  Cloud  Feature  that  Generates  Extreme Weather in the Java Island Region
Abstrak: Overshooting Top (OT) is a feature at the top of a convective cloud that is associated with extreme weather. Research on OT in tropical Indonesia is still very limited. The existence of geostationary satellites with wide area coverage and fast frequency observations is useful for observing the fast dynamics of the OT life cycle. Therefore, this study intends to analyze the relationship between OT and extreme weather, updraft and thermodynamic conditions of OT when extreme weather occurs, the best objective method for detecting OT in the day time and night time, and the spatiotemporal distribution of OT in the tropical region of Java Island during the 2018-2021 period. Based on visual analysis using visible channel and Himawari 8 sandwich products, 87% of extreme weather events in Java were accompanied by the appearance of OT. It indicates that the presence of the OT pattern is a convection cloud pattern or feature that is closely related to extreme weather events for the Java Island region. The existence of a time lag between the appearance of OT and extreme weather events on Java Island indicates that the OT of convection cloud can be used as a precursor or nowcasting for the appearance of extreme weather. Most of the convective cloud OT features during extreme weather events on Java Island have altitude that were only able to exceed the altitude of the equilibrium level and few were able to exceed the tropopause level of 9 km – 16 km. The difference in atmospheric conditions during extreme weather events on Java Island, which is in the tropics and subtropics, is a strong and unstable convective energy condition characterized by a larger CAPE, as well as unstable KI, LI, SI and TTI values. It is offset by kinematic factors in the form of a weak vertical wind shear (bulk wind shear 0-6 km) and a higher freezing point height. The difference between extreme weather events in the Java Island region and other tropical regions compared to subtropical regions is the strong updraft speed of convection clouds. The IRW Texture COMB algorithm based on the IRW-Texture Gradient modification and the COMB algorithm shows a significant increase in quality for detecting OT and non-OT pixels in Java. Rainy days with rainfall > 50 mm/day are very common in areas with an altitude of > 500 m. On the other hand, the level of convection activity which is indicated by the presence of OT and lightning is significantly reduced in this area. OT of convective clouds appear more frequently during the DJF season and less frequently during the JJA season. OT often appears during the day time and rarely at night time. Most OTs appear in the central part, away from the south and north coasts of Java Island during the day, while OTs that appear near the south and north coasts of Java Island can appear in the morning or afternoon.
Keyword: convective clouds, extreme weather, Overshooting Tops, Himawari 8 Satellite