Judul: Praktek Sistem Gadai Sawah Masyarakat Desa
Abstrak: Tujuan penelitian ini adalah (1) Menelaah berbagai penyebab mengapa petani menggadaikan sawahnya; (2) Menelaah seluk beluk praktek sistem gadai sawah; (3) Menganalisis dampak praktek sistem gadai sawah terhadap perubahan jenis pekerjaan, tingkat pendapatan penggadai, penebusan kembali sawah gadai, dan munculnya konflik sengketa gadai.
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Judul: Dampak sosial pencetakan sawah pada areal rawa: Kasus di desa Mujur, Kecamatan Kroya, Kabupaten Cilacap, Propinsi Jawa Tengah
Abstrak: Penelitian ini dilakukan pada proyek pencetakan sawah secara swadaya pada lahan rawa di Desa Mujur, Kecamatan Kroya, Kabupaten Cilacap, Propinsi Jawa Tengah, dengan tujuan untuk mengetahui dampak lingkungan sosial, baik dampak positif maupun dampak negatif yang terjadi akibat adanya proyek pencetakan sawah. Untuk mencapai tujnan tersebut, maka dilakukan pengamatan Japang selarna kurang lebih sebulan. Data primer diperoleh dari wawancara dengan petani, infonnan dan pengamatan langsung di lapang. Data sekunder yang dikumpulkan meliputi keadaan umum lokasi penelitian, data sarana dan prasarana yang ada, tingkat ketersediaan air, kondisi fisik jaringan irigasi, kelembagaan yang berkaitan dengan pengelolaan air dan jaringan irigasi, status penguasaan lahan, topografi, kondisi tanah, dan data produksi usahatani. Data yang telah siap diolah kemudian dianalisis dengan menggunakan analisis kuantitatif yaitu menggunakan tabel frekuensi, dan analisis kualitatif dengan mendeskripsikan berbagai fenomena yang te1jadi yaitu dengan membandingkan data keadaan sebelum dan susudah adanya pencetakan sawah.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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