Judul: Pengoptimuman Fase Gerak KLT dengan Rancangan Campuran untuk Analisis Sidik Jari Temulawak
Abstrak: Temulawak is widely used as jamu, a traditional Indonesia medicine. Therefore we have developed a TLC fingerprint method to standardized raw material or extract of Curcuma xanthorrhiza to ensure the quality of its finished product. The fingerprint of TLC analysis is a common method for this purpose. First step to developed a TLC fingerprint was to select optimum mobile phase to obtained good separation of the components. Simplex centroid design is used for mobile phase optimization from three selected solvents that were acetone, dichloromethane, and chloroform. The results showed that the optimum mobile phase using UV 366 nm detection was obtained using dichloromethane and chloroform ratio of 0.5164:0.4836 with R2 value of 91.39%, whereas the optimum mobile phase using vanillin-sulfuric acid detection was obtained using dichloromethane and chloroform ratio of 0.8178:0.1822 with R2 value of 93.40%.
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Judul: Diferensiasi Temulawak, Kunyit, dan Bangle Berdasarkan Pola Pemisahan Senyawa Menggunakan Kromatografi Lapis Tipis
Abstrak: Differentiation of herbal medicine is very important to authentication test of raw materials and adulteration detection. Temulawak, kunyit, and bangle are herbs which are required in large scale. These plants are usually sold in powder form, so it would be difficult to differentiate them visually. Thus, a differentiation method to evaluate chemical composition of these three plants must be found. Thin layer chromatography (TLC) is a method frequently used for differentiation of herbs. The results showed that the best eluent for doing separation of the chemical compound content of the sample was dichloromethane:chloroform = 32.5:67.5. This eluent was used to separate chemical compounds of herbs on TLC plates. The specific compounds were found differently on TLC plates. TLC results with visible light and ultraviolet detection at a wavelength of 254 and 366 nm indicated that each of the eight areas of medicinal plants in Java has a content characterized by the same band. Based on the characteristic band, temulawak contain two major components of curcuminoids, namely curcumin (Rf 0.4) and demethoxycurcumin (Rf 0.16), kunyit contains three major components namely curcumin (Rf 0.4), demethoxycurcumin (Rf 0.16), and bisdemethoxycurcumin (Rf 0.06), while the bangle contains curcumin (Rf 0.4) and other compounds with Rf 0.6 and Rf 0.31.
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Judul: Kejadian Kasus Newcastle Disease (ND) pada Peternakan Ayam Buras di Kabupaten Barru Provinsi Sulawesi Selatan Tahun 2015–2017
Abstrak: Newcastle disease (ND) merupakan penyakit menular pada ayam dengan angka kematian yang tinggi. Kerugian ND dapat berupa kematian ayam dan penurunan produksi telur. Penelitian ini bertujuan menganalisis data kasus, mengukur kecepatan penyebaran penyakit, menggambarkan distribusi penyakit, dan memetakan wilayah yang berisiko terjadi penyebaran kejadian ND. Data penelitian diperoleh dengan cara wawancara mendalam menggunakan kuesioner terstruktur kepada pihak dinas. Data tersebut diolah dengan menghitung incidence rate dan memetakan wilayah berisiko menggunakan geographic information system (GIS). Hasil penelitian menunjukkan bahwa incidence rate tertinggi di 7 kecamatan selama 3 tahun adalah di Kecamatan Soppeng Riaja sebesar 14 kasus per 100 000 ekor—tahun, sedangkan incidence rate terendah adalah di Kecamatan Tanete Riaja sebesar 0 kasus per 100 000 ekor—tahun. Kecamatan dengan risiko tinggi adalah Kecamatan Soppeng Riaja, sehingga tindakan pengendalian perlu ditekankan pada daerah tersebut.
Keyword: Newcastle disease (ND), incidence rate, ayam buras, Kabupaten Barru