Judul: Business Model Design and Financial Analysis for Sweet Premix Product at PT LeGitt Boga Mandiri, Bogor
Abstrak: PT LeGitt Boga Mandiri sebagai perusahaan makanan berbasis tepung terigu sedang mengembangkan produk baru yaitu tepung sweet premix corn dog. Agar proses pengembangannya berjalan dengan lancar, dibutuhkan perancangan model bisnis yang tepat dan juga analisis finansial guna mengetahui apakah produk tersebut tepat dan layak untuk diluncurkan oleh PT LeGitt Boga Mandiri. Perancangan model bisnis dilakukan dengan pendekatan Lean Canvas. Analisis finansial dilakukan dengan metode discounted cashflow. Harga Pokok Produksi dari produk ini adalah Rp. 22.513/Kg dan hasil dari perhitungan analisis finansial didapat: (1) Nilai NPV Rp. 106.604.854, (2) IRR 58%. Dari segi kelayakan, produk ini layak untuk diteruskan oleh PT LeGitt Boga Mandiri karena setelah ditinjau dari aspek finansial maupun non finansial, produk ini layak dan tidak sensitif terhadap perubahan harga bahan baku utama., PT LeGitt Boga Mandiri, a flour-based food manufacturing company, is currently developing a new product. The product is a premix flour for making corn dog. To ensure a smooth development process requires a proper business model design and financial analysis to determine whether the product is suitable and feasible for launch by PT LeGitt Boga Mandiri. The business model is designed using Lean Canvas, and the financial analysis is conducted using discounted cashflow method. The Total Manufacturing Cost (TMC) for this product is Rp.22.513/Kg, and the results of the financial analysis are as follows: (1) NPV value of Rp.106.604.854, (2) IRR of 58%. From a feasibility perspective, this product is suitable to be adopted by PT LeGitt Boga Mandiri because, after considering both financial and non-financial aspects, the product is feasible and insensitive to changes in main raw material prices.
Keyword: Business Model, Financial Analysis, Lean Canvas, Net Present Value (NPV), Product Development

Judul: Development of Sweet Premix Flour at LeGitt Boga Mandiri Company
Abstrak: Tepung sweet premix adalah salah satu jenis tepung premix yang berasal dari campuran bahan-bahan yaitu gula, susu, tepung, bubuk essence, dan bahan lainnya dengan tujuan meningkatkan efisiensi dalam penggunaannya. Tepung sweet premix ini yang kemudian diaplikasikan menjadi corn dog. Corn dog merupakan produk yang berasal dari adonan atau batter yang diaplikasikan ke permukaan isian yaitu sosis dan melalui proses penggorengan dengan menambahkan tepung roti pada permukaan batter yang telah diaplikasikan. Desain proyek ini terdiri dari prototyping 0,1,2, dan 3. Pengujian pada setiap prototipe terdiri uji organoleptik, daya lekat, densitas, dimensi, kadar air tepung, dan kadar lemak produk kemudian dari seluruh pengujian tersebut dilakukan rankingan produk dengan metode indeks efektivitas dengan bobot parameter disesuaikan dengan target parameter pada prototipe 0-3. Prototipe 0-3 masing-masing dengan tiga iterasi dengan target parameter prototipe 0 produk terbaik dari tiga sumber yaitu dari competitor dan studi literatur diperoleh perlakuan terbaik yaitu prototipe A(UMKM) sampel kompetitor, prototipe 1 target parameter daya lekat diperoleh ranking produk yaitu prototipe 1A dengan nilai daya lekat sebesar 4,26, prototipe 2 target parameter tekstur diperoleh ranking produk terbaik yaitu prototipe 2C dengan nilai tekstur 4,43, dan prototipe 3 target parameter rasa diperoleh ranking produk terbaik yaitu prototipe 3C dengan nilai rasa berdasarkan uji organoleptik sebesar 4 dan berdasarkan uji beda nyata prototipe 3 dengan 3 iterasi dibandingkan dengan prototipe kontrol (2C) tidak berbeda nyata maka pengaruh komposisi susu tidak berpengaruh terhadap kesukaan konsumen terhadap rasa. Prototipe akhir yang terpilih dilakukan pengujian karakteristik gizi produk tepung sweet premix yaitu diperoleh hasil pengujian yaitu 8,6% kadar air, 0,73% kadar abu, 9,43% kadar lemak, 4% kadar protein, 13% kadar serat, dan 64,24% kadar karbohidrat., Sweet premix flour is a type of premix flour that comes from a mixture of ingredients, namely sugar, milk, flour, essence powder, and other ingredients with the aim of increasing efficiency in its use. This sweet premix flour is then applied to corn dogs. Corn dog is a product that comes from a dough or batter that is applied to the surface of the filling, namely sausages and goes through a frying process by adding bread flour to the surface of the batter that has been applied. The project design consists of prototyping 0,1,2, and 3. Tests on each prototype consisted of organoleptic tests, adhesion of bread to sausages, density, dimensions, flour moisture content, and product fat content. Then from all these tests, product ranking was carried out using the effectiveness index method with parameter weights adjusted to the target parameters on prototype 0-3. Prototype 0-3 each with three iterations with the target parameter prototype 0 the best product from three sources, namely from competitors and literature studies obtained the best treatment, namely prototype A (MSMEs) competitor sample, prototype 1 target parameter of adhesion obtained product ranking, namely prototype 1A with a stickiness value of 4.26, prototype 2 target texture parameter obtained the best product ranking, namely prototype 2C with a texture value of 4.43, and prototype 3 target parameter taste obtained the best product ranking, namely prototype 3C with a taste value based on an organoleptic test of 4 and based on
Keyword: Corn dog, Product development, Prototyping, Sweet premix flour

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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