Judul: Fraksi aktif ekstrak daun sansevieria trifasciata lorentii sebagai penghambat proliferasi sel lestari HeLa
Abstrak: Daun lidah mertua (Sansevieria sp) telah banyak diteliti, terutama khasiatnya sebagai antioksidan dan antibakteri, sedangkan khasiat sebagai antikanker belum pernah dilaporkan. Oleh karena itu, tujuan penelitian ini adalah mengisolasi fraksi aktif ekstrak daun Sansevieria trifasciata yang dapat menghambat proliferasi sel lestari HeLa secara in vitro. Teknik ekstraksi dilakukan secara maserasi dalam metanol 96% dan dipekatkan menggunakan penguap putar. Ekstrak metanol difraksinasi menggunakan kolom kromatografi melalui proses elusi gradien dengan nisbah pelarut kloroform dan metanol. Uji fitokimia dan uji penghambatan proliferasi sel lestari HeLa dengan metode MTT [3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromida] dilakukan terhadap ekstrak kasar dan hasil fraksinasi pada konsentrasi antara 10-100 ppm. Rendemen ekstrak kasar daun lidah mertua diperoleh sebesar 18% dan mengandung flavonoid, saponin, dan steroid. Nilai daya hambat proliferasi sel lestari HeLa tertinggi untuk ekstrak kasar diperoleh sebesar 23% pada konsentrasi 10 ppm. Hasil uji hambat yang teraktif dari sepuluh fraksi adalah 41% pada konsentrasi 100 ppm dan mengandung metabolit golongan steroid.
Keyword: HeLa cell line, Kromatografi kolom, Bogor Agricultural University

Judul: Uji Aktivitas Antibakteri Fraksi Daun Lidah Mertua (Sansevieria trifasciata)
Abstrak: Sansevieria trifasciata is an indigenous plant of South Africa used in herbal medicine for the treatment of various infectious diseases. The purpose of this study is to evaluate potency of S. trifasciata extracts through in vitro antibacterial test against Grampositive (Staphylococcus aureus) bacteria and Gram-negative (Escherichia coli) bacteria on Mueller-Hinton medium. The plant sample were obtained from research garden at Lodaya Campus, Bogor Agricultural University. The result indicated that methanol crude extract at concentration 10000, 5000, 2500, and 1250 ppm showed no activity against the tested bacteria. Methanol crude extract was fractionated using coloumn chromatography with gradient method. Methanol and chloroform were used as eluent with the best ratio of 9:1. The fractionation yielded 10 fractions. The fractions were tested at concentration 40000, 20000, and 10000 ppm. The antibacterial assay showed there were 2 active fractions for both of the tested bacteria, 5 active fractions against only Gram-positive (S. aureus) and 3 non-active fractions.
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Judul: The impact and contribution of the tourism sector to the Indonesian economy : an I-O and SAM approach
Abstrak: Tourism is an important and strategic sector for the Indonesian economy. The sector has been growing for the last two decades to enhance its contribution to the GDP, employment, regional, local economy, and to strengthen the balance of payment. In particular, a planned economy was introduced to provide substantial opportunities to utilize tourism as a mechanism for national development. As a service-based sector, tourism as also considered to be a vehicle of converting the manufacturing economy to a service economy which makes the delivery of the product rather than the creation of the product the most important thing.
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