Judul: Landscape Design of Bendi Park as Outdoor  Sports and Play Area
Abstrak: Tingginya jumlah penduduk dengan padatnya bangunan di perkotaan terutama Jakarta menyebabkan tingginya kebutuhan ruang terbuka hijau dalam suatu wilayah. Taman Bendi sebagai salah satu taman di Jakarta memiliki ruang terbuka hijau yang saat ini kondisinya belum optimal dari segi fasilitas dan penggunaannya. Penelitian ini bertujuan membuat desain lanskap Taman Bendi sebagai area olahraga outdoor dan bermain di Kecamatan Kebayoran Lama dan sekitarnya. Penelitian ini dilakukan dengan menggunakan metode kualitatif dan kuantitatif. Metode kualitatif adalah penelitian yang menghasilkan data deskriptif berupa kata-kata tertulis dari orang orang dan perilaku yang dapat diamati. Dalam pendekatan kualitatif fokus terhadap aspek fisik dan biofisik. Metode analisis kuantitatif merupakan penelitian ilmiah yang sistematis terhadap bagian-bagian dan fenomena serta kualitas hubungan hubungannya. Berdasarkan pendekatan kuantitatif, data hasil kuesioner diolah menggunakan metode cross tabulation untuk mengetahui pengaruh rentang usia terhadap persepsi dan preferensi desain. Hasil penelitian ini berupa site plan dengan desain yang dapat memaksimalkan potensi dan memberikan solusi permasalahan yang ada di Taman Bendi, planting plan, gambar potongan, dan gambar perspektif., The high number of residents with the density of buildings in urban areas, especially Jakarta, causes a high need for green open space in an area. Bendi Park as one of the parks in Jakarta has green open space which is currently not optimal in terms of facilities and use. This study aims to design the landscape of Bendi Park as an outdoor sports and play area in Kebayoran Lama District and its surroundings. This research was conducted using qualitative and quantitative methods. Qualitative method is research that produces descriptive data in the form of written words from people and observable behavior. In the qualitative approach the focus is on physical and biophysical aspects. The method of quantitative analysis is a systematic scientific study of the parts and phenomena and the quality of their relationships. Based on the quantitative approach, the questionnaire data were processed using the cross tabulation method to determine the effect of age range on design perceptions and preferences. The results of this study are in the form of a site plan with a design that can maximize potential and provide solutions to problems that exist in Bendi Park, planting plan, section drawings, and perspective pictures.
Keyword: Area olahraga dan bermain, desain lanskap, ruang terbuka hijau, taman  bendi

Judul: Redesign of Lembah Gurame Park as an Educational Based Urban Park in Depok City
Abstrak: Taman kota merupakan ruang terbuka hijau publik yang memiliki peran sebagai tempat rekreasi, edukasi, dan aktivitas masyarakat sehingga keberadaannya perlu mampu memfasilitasi setiap kegiatan tersebut. Taman Lembah Gurame memiliki luas sekitar 3 hektar merupakan taman kota yang berfungsi sebagai ruang terbuka hijau publik di Kota Depok. Namun, fasilitas yang tersedia dinilai masih kurang memfasilitasi pengguna seperti, kurangnya tempat duduk, kurangnya fasilitas bagi penyandang disabilitas, dan kurangnya lahan parkir. Selain itu, terdapat beberapa masalah terkait penataan taman seperti vegetasi yang belum tertata rapih dan banyaknya elemen yang rusak pada area timur taman sehingga sepi pengunjung. Keberadaan Taman Lembah Gurame yang berdekatan dengan bangunan sekolah-sekolah membuat sebagian besar pengunjungnya merupakan anak-anak sehingga taman berpotensi untuk dikembangkan menjadi taman kota berbasis edukasi. Tujuan dari penelitian ini adalah untuk mengidentifikasi dan menganalisis fisik, biofisik, dan karakter taman, menganalisis aktivitas edukasi pengunjung, serta menyusun rekomendasi desain taman kota berbasis edukasi. Metode yang dilakukan dalam penelitian ini mencakup tahap persiapan, pengumpulan data, analisis dan sintesis, serta membuat konsep dan desain. Pengembangan tapak menjadi taman kota berbasis edukasi berfokus pada kegiatan seperti observasi alam, permainan edukatif, dan pembelajaran di luar ruangan. Konsep dasar yang diterapkan adalah "Experience the living masterpiece of nature's canvas," yang bertujuan menjadikan tapak sebagai fasilitas untuk rekreasi, edukasi, dan konservasi, dengan memberikan pengalaman langsung bagi pengunjung untuk menikmati alam. Desain yang diterapkan diadaptasi dari bentuk buah belimbing yang dimaknai sebagai kelima indra dan ditransformasi menjadi pola geometrik-organik. Pengembangan ruang terbagi menjadi edukasi, rekreasi, penerimaan, pelayanan, dan konservasi. Ruang edukasi terdiri dari edukasi aktif dan edukasi pasif. Ruang tersebut berfungsi sebagai ruang dengan fasilitas yang mendukung aktivitas edukasi. Aktivitas edukasi mencakup belajar di alam dan observasi vegetasi serta satwa. Pengembangan ini diharapkan memberikan masukan dalam mengembangkan taman kota berbasis edukasi., The urban park serves as a public green open space for recreation, education, and other community activities so that its facilities needs to support these function. Lembah Gurame Park, covering approximately 3 hectares, is a significant green space in Depok City. However, it currently lacks adequate seating, facilities for people with disabilities, and sufficient parking. Additional issues include poorly organized vegetation and numerous damaged elements in east area of the park, leading to lack of visitors. The existence of Lembah Gurame Park which is adjacent to school buildings makes most of the visitors are children so the park has the potential to be developed into an education-based urban park. The purpose of this research is to identify and analyze the physical, biophysical, and characteristics of the park, analyze the educational activities of visitors, and develop recommendations for the design of education based urban parks. The method used in this research includes the preparation stage, data collection, analysis and synthesis, and making concepts and designs. The site transformation into an educational urban park focuses on activities like nature observation, educational play, and outdoor learning. The fundamental design concept of Lembah Gurame Park is "Experience the living masterpiece of nature's canvas," aiming to make the site as a facility for recreation, education, and conservation, providing visitors with direct experiences to enjoy the nature. The design is inspired by
Keyword: Desain Lanskap, taman kota, Ruang Terbuka Hijau Publik, Redesain, Taman Edukasi

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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