Judul: Pemanfaatan Metode PCA dalam Analisis Sidik Jari dan Uji Kandungan Andrografolid Tanaman Gandarusa (Justicia gendarussa Burm. F.).
Abstrak: Gandarusa (Justicia gendarussa Burm. F.) merupakan tanaman herbal yang digunakan dalam pengobatan tradisional karena mengandung senyawa metabolit sekunder. Proses pembentukan senyawa metabolit sekunder dipengaruhi oleh faktor ketinggian, suhu, kelembapan, tekanan udara, gravitasi, curah hujan, dan komponen tanah. Penelitian ini bertujuan melakukan analisis metode optimal, sidik jari, dan kandungan andrografolid tanaman gandarusa menggunakan HPLC. Hasil penelitian menunjukkan kondisi optimum analisis gandarusa yaitu panjang gelombang 254 nm, sistem gradien metanol:akuabides, laju alir 2 mL/menit, waktu operasi 35 menit, dan volume injeksi 20 μL. Hasil analisis sidikjari gandarusa terbagi menjadi 2 klaster, klaster 1 terdiri atas seluruh sampel asal NTB, NTT, Kalimantan, Maluku, dan Papua Barat kecuali Marai NTT yang tegolong kedalam klaster 2. Kandungan andrografolid tertinggi pada gandarusa berada di Marai NTT sebanyak 3886.07 μg/g ekstrak.
Keyword: andrographolide, chemical fingerprint, Justicia gendarussa, willow- leaved justicia

Judul: Flavonoid Content and Antioxidant of Gandarusa (Justicia gendarussa Burm. f.) Methanol Extract Cultivated with Manure and NPK
Abstrak: Gandarusa (Justicia gendarussa Burm. f.) merupakan salah satu tanaman obat yang berpotensi sebagai antioksidan karena adanya kandungan metabolit sekunder. Kandungan metabolit sekunder dan antioksidan pada tanaman dapat dipengaruhi oleh kondisi lingkungan tumbuh tanaman tersebut, salah satunya pemberian pupuk. Penelitian ini bertujuan menentukan teknik budidaya yang tepat menggunakan komposisi pupuk kandang dan NPK terhadap tanaman gandarusa, dengan menganalisis kadar flavonoid dan aktivitas antioksidan secara in vitro. Pupuk yang digunakan adalah kombinasi pupuk kandang dan NPK dengan dilakukannya enam perlakuan. Total flavonoid diukur menggunakan metode kuantitatif spektrofotometri, sedangkan aktivitas antioksidan ditentukan menggunakan metode CUPRAC dan ABTS. Hasil menunjukkan pemberian 50% pupuk kandang dan 50% NPK memberikan hasil total flavonoid tertinggi pada daun sebesar 4,5690 mg/g, dan berkorelasi positif serta signifikan terhadap total antioksidan metode ABTS pada daun sebesar 15,089 µmol/g., Gandarusa (Justicia gendarussa Burm. f.) is a medicinal plant that has the potential as an antioxidant due to the presence of secondary metabolites. The content of secondary metabolites and antioxidants in plants can be affected by the environmental conditions where the plants growth, such as application of fertilizers. This research aims to determine the appropriate cultivation technique using the composition of manure and NPK on gandarusa, by analyzing the levels of flavonoids and antioxidant activity with in vitro method. The fertilizers used were a combination of manure and NPK with six treatments. Total flavonoids content was determined quantitative spectrophotometric methods, and the antioxidant activity use CUPRAC and ABTS methods. The results showed the application of 50% manure and 50% NPK gave the highest total flavonoid yields in gandarusa leaves 4,569 mg/g and had a positive and significant correlation with the total antioxidant ABTS method on leaves 15,089 µmol/g.
Keyword: Antioksidan, Flavonoid, Gandarusa, NPK, Pupuk Kandang

Judul: Dynamical System of Hepatitis B in Three Groups with Vaccination
Abstrak: The aim of this study is to modify the hepatitis B transmission model in three age groups involving vaccination from the model conducted by Zhoe and Zhang.The method used is to analyze the stability of the fixed point and its basic reproduction number (R_0). Performed stability analysis and numerical simulation to see population dynamics. Next, a sensitivity analysis of the parameters was carried out to see its effect on R_0. From the analysis obtained two fixed points, namely non-endemic and endemic fixed points. Non-endemic fixed points are locally asymptotically stable if R_0 <1 Meanwhile, the endemic fixed point is locally asymptotically stable if R_0 > 1. Hepatitis vaccination affects the number of susceptible population, because the more successful the vaccination, the smaller the number of susceptible populations so that the smaller the population will be infected.
Keyword: Dynamic, Hepatitis B, Vaccine, Mathematical model