Judul: Identification of pituitary transcription factor 1 (POU1F1) gene in etawah grade goat (PE) in BPTU KDI-HPT pelaihari.
Abstrak: Pituitary transcription factor 1 (POU1F1) merupakan faktor transkripsi khusus yang mengatur ekspresi gen penyandi hormon pertumbuhan dan hormon prolaktin. Penelitian ini bertujuan untuk mengidentifikasi polimorfisme gen POU1F1 pada kambing peranakan etawah (PE) di BPTU KDI-HPT Pelaihari Kalimantan Selatan. Amplifikasi gen POU1F1 dengan suhu annealing 62oC selama 20 detik menghasilkan fragmen dengan panjang 601 pb yang terletak pada intron 5 dan exon 6. Produk PCR gen POU1F1 dipotong menggunakan enzim Hinf1 yang memotong sekuen basa G|AnTC. Hasil analisis PCR-RFLP menunjukkan gen POU1F1 pada sampel bersifat monomorfik, ditemukan 1 tipe alel yaitu tipe B. Alel B mempunyai 3 potongan fragmen masing masing pada 275, 243, dan 83 pb.
Keyword: kambing PE, PCR-RFLP, Pituitary Tanscription Factor 1 (POU1F1)

Judul: Identifikasi Keragaman Gen Insulin-like Growth Factor-1 pada Kambing Peranakan Etawah di BPTU KDI-HPT Pelaihari Kalimantan Selatan
Abstrak: Etawah grade goats meat productions can be improved through the efforts of livestock genetic enhancement based on the exploration of potential genes that supposedly strong influence in growth trait. Insulin-like growth factor-1 (IGF-1) gene is known to be associated with the milk production and growth traits. The objective of this research was to identify the polymorphism of IGF-1 gene in Etawah grade goats from BPTU KDI-HPT Pelaihari South Kalimantan. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method used to detect the genotype of IGF-1 gene. Polymorphism in 5’-flanking region of IGF-1 gene was studied in 115 Etawah grade goats blood samples. A primer designed specifically was used to amplify a fragment DNA of IGF-1 locus along 249 bp. Restriction with SnaBI revealed one allele B was cut at one restriction site in fragment 249 bp. The frequencies for allele B was 1.00, resulting monomorphic allele. Based on Ge et al. (2001) and Siadkowska et al. (2006) researchs, allele B was associated on weight and body size.
Keyword: polymorphism, PCR-RFLP, IGF-1 gene, Etawah grade goats, Bogor Agricultural University (IPB)

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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