Judul: Respons benih ikan sidat terhadap hormon pertumbuhan rekombinan ikan kerapu kertang melalui perendaman dan oral
Abstrak: This study was aimed to determine an appropriate administration method and observe the response of eel fingerling on recombinant giant grouper growth hormone (rElGH). First study aimed to determine the immersion dose of rElGH to increase growth of eel juvenile (glass eel). After shock salinity treatment (NaCl 3% for 2 min), glass eel were immersed in water containing 0.9% NaCl, 0.01% bovine serum albumin, and different of dose of rElGH (0, 0.12, 1.2, 12 and 120 mg/L). The results showed that higher in growth body weight was obtained in treatment 12 mg/L (101.2±0.8), by increment of about 37.4% higher compared to that of control (72.93±1.8). Second study was aimed to determine the dose of rElGH mixed in artificial diet to increase growth of eel elver. HP55-coated rElGH was mixed to diet at different doses (0, 0.3, 3, and 30 mg/kg). Elver was fed on diet containing rElGH twice daily, 6% fish biomass. The result showed that higher in growth body weight was obtained in treatment 30 mg/kg (60.18±1.38), by increment of about 65.7% compared to control (36.32±0.97). Third study was aimed to compared of three administration method, namely immersion, oral, and combination of immersion and oral. The dosage of rElGH by immersion (12 mg/L) and oral administration (30 mg/kg feed) was obtained in previous study. Fish were maintained in 23-L glass aquaria for 120 days, at density of 45 fish per aquarium. The results showed that higher (P<0.05) biomass of harvest (73.68±2.07 g) was obtained in immersion and oral combination treatment, by increment of about 102.9% compared to control (36.32±0.97 g). Administration of rElGH also improved protein and lipid retentions by 142.5%, and 720% compared to that of control, respectively (P<0.05). In addition, rElGH treatment increased appetite, while artificial feed conversion ratio (4.75) was lower (P<0.05) by 55.15% compared to that of control (7.37). Combination of rElGH immersion and oral administrations also increased hepatosomatic index, reduced ammonia excretion and increased insuline-like growth factor-1/IGF-1 gene expression. Thus, rElGH administration via immersion and oral was a proper method to improve performance of eel fingerling, and application of rElGH can be useful to increase aquaculture production.
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Judul: Respons benih ikan gurame (Osphronemus goramy) yang diberi hormon pertumbuhan rekombinan melalui oral pada dosis berbeda
Abstrak: This study was aimed to evaluate the response of giant gourami juvenile on oral administration of recombinant giant grouper growth hormone (rElGH). A total of 45 juvenile were fed with rElGH coated by hydropropylmethyl cellulose phthalate (HP55) and mix with commercial diet (35% protein content). Feeding fish with diet containing four different doses of rElGH (0 mg/kg feed, 0.3 mg/kg feed, 3.0 mg/kg feed, and 30.0 mg/kg feed) was performed in twice a week for 2 month. Fish were then maintained for 1 month without rElGH treatment. The results showed that biomass harvest in treatment 3.0 mg/kg (1,376.0±38.3 g) and 30.0 mg/kg (1,362.2±78.5 g) was similar, and the two treatments were 46.76% and 44.28% higher compared to control (977.6±96.7 g), respectively. In addition, rElGH treatment increased appetite, while artificial feed conversion ratio (1.18) was lower (P<0.05) by 23.72% compared to that of control (1.46). Oral administration of rElGH also increased hepatosomatic index (16.12%) and protein content (17%), while lipid content (21%) decreased compared to control. Similar in fish survival at end of experiment, insulin-like growth factor-1 and growth hormone receptor-1 gene expression levels in brain at 24 hours after feeding in rElGH treatment and control were obtained. Thus, orally rElGH administration was effective to be applied to enhance the growth of giant gourami juvenile.
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Judul: Closed Knight’s Tour pada Papan Catur Berbentuk Persegi Panjang dengan Ukuran Maksimal 8 × 8
Abstrak: Closed Knight’s Tour merupakan langkah kuda yang mengunjungi setiap kotak pada papan catur tepat satu kali dengan langkah kuda berawal dan berakhir pada kotak yang sama. Graf yang terbentuk dari closed knight’s tour dinotasikan sebagai graf G(m,n), dengan m adalah banyaknya baris dan n adalah banyaknya kolom. Graf tersebut memuat Hamiltonian cycle. Sebuah graf terhubung G adalah graf bipartite jika dan hanya jika graf tersebut tidak memiliki cycle dengan panjang ganjil. Papan catur yang memenuhi syarat cukup untuk memiliki closed knight’s tour adalah papan catur berukuran 5 × 6, 5 × 8, 6 × 6, 6 × 7, 6 × 8, 7 × 8, dan 8 × 8.
Keyword: bipartite, closed knight’s tour, hamiltonian cycle, knight’s tour, teori graf