Judul: Evaluation of The Implementation and Development of Green City Concept in South Tangerang City
Abstrak: Sebagai hasil dari pemekaran Kabupaten Tangerang, Kota Tangerang Selatan merupakan salah satu dari kota-kota berusia belia di Indonesia. Lokasi Kota Tangerang Selatan yang secara strategis berdekatan dengan kota metropolitan Jakarta membuat kota suburban ini menjadi pilihan yang tepat untuk pemukiman. Setelah pemisahan diri dari Kabupaten Tangerang, Kota Tangerang Selatan telah berkembang pesat terutama dalam aspek infrastruktur dan komplek pemukiman. Perkembangan ini juga turut berdampak terhadap berubahnya kualitas lingkungan kota. Oleh karena itu, konsep kota hijau hadir untuk menjadi standarisasi bagi usaha-usaha berkelanjutan yang dilakukan untuk menyetarakan kondisi lingkungan sebuah kota dan kualitas hidup masyarakat. Penelitian ini bertujuan untuk mengevaluasi ketersediaan ruang terbuka hijau dengan penginderaan jarak jauh dan implementasi konsep kota hijau Kota Tangerang Selatan dengan menggunakan Asian Green City Index untuk mengetahui perkembangan dan pertumbuhannya. Penelitian dilaksanakan secara temporal pada tiga titik tahun pengamatan, yakni tahun 2010, 2015, dan 2020. Hasil evaluasi menunjukkan adanya peningkatan dalam ketersediaan ruang terbuka hijau di Kota Tangerang Selatan. Pada tahun 2010, luas ruang terbuka hijau adalah sebesar 3.518 ha (21,34%), kemudian pada tahun 2015 meningkat menjadi 4.017 ha (24,37%), dan mencapai kriteria ketersediaan ruang terbuka hijau pada tahun 2020 yakni seluas 7.325 ha (44,43%). Sejalan dengan tren peningkatan ketersediaan ruang terbuka hijau, hasil evaluasi performa Kota Tangerang Selatan dalam menerapkan konsep kota hijau juga menunjukkan adanya peningkatan setiap tahunnya. Pada tahun 2010, performa penerapan konsep kota hijau tergolong pada kategori di bawah rata-rata (below average) dengan skor sebesar 25,61%. Kemudian di tahun 2015 dan 2020, performa penerapan konsep kota hijau mengalami peningkatan hingga menjadi kategori rata-rata (average) dengan skor sebesar 44,93% dan 59,75%., As the result from Tangerang Regency’s expansion, South Tangerang stands as one of the youngest cities in Indonesia. South Tangerang’s location that is strategically located close to the metropolitan city Jakarta, makes the suburban city a desirable choice for residence. After the separation, the city has been developing in terms of infrastructure and housing complexes. The development itself has since imprinted several impacts on the city’s environment quality. Therefore, the green city concept exists to standardize sustainable efforts made to balance a city’s environment condition and the population’s quality of life. This study aims to evaluate the availability of green open spaces using remote sensing and the implementation of the green city concept in South Tangerang City by utilizing the Asian Green City Index to assess its development and growth. The study is held temporally within three years of observation periods, namely year 2010, 2015, and 2020. The evaluation results in an escalation in South Tangerang City's green open space availability. In 2010, the green open space area reached 3.518 ha (21,34%), then in 2015 ascended to 4.017 ha (24,37%), and eventually able to reach the green open space's availability criteria in 2020 with an area of 7.325 ha (44,43%). Aligning with the green open space's availability's escalation trend, the green city concept implementation evaluation also showcases an escalation on every observation periods. In 2010, the green city concept implementation performance is considered below average with an Asian Green City Index score of 25,61%. Afterwards, in 2015 and 2020, the green city
Keyword: South Tangerang City, green city concept, asian green city index, development

Judul: EVALUATION OF GREEN CITY ON GREEN OPEN SPACE IN TANGERANG CITY
Abstrak: Kota Tangerang merupakan sebuah wilayah di Provinsi Banten yang mengalami perubahan penggunaan lahan karena meningkatnya angka jumlah penduduk. Di Tengah permasalahan tersebut, kota hijau hadir sebagai konsep pengembangan kota yang berkelanjutan. Sejalan dengan perkembangan di Kota Tangerang, upaya pemerintah dalam mewujudkan kota hijau perlu dianalisis untuk mengetahui kinerja kota. Tujuan utama penelitian ini adalah mengevaluasi penerapan konsep kota hijau di Kota Tangerang. Secara makro, evaluasi dilakukan dengan menganalisis perubahan tutupan lahan menggunakan penginderaan jauh. Secara mikro, kualitas ruang terbuka hijau juga dianalisis dengan mengidentifikasi RTH Publik terpilih berdasarkan kriteria 8 atribut kota hijau. Hasil penelitian menunjukkan bahwa kawasan terbuka hijau di Kota Tangerang terus mengalami penurunan dari tahun 2009 sebesar 38,29%, lalu pada tahun 2016 sebesar 28,07%, hingga tahun 2023 sebesar 21,84%. Di sisi lain, persentase penerapan atribut kota hijau pada Alun-alun Cibodas dan Hutan Kota Tangerang berurutan sebesar 48,30% dan 57,28% menandakan kualitas kedua RTH tersebut masih kurang baik. Selain menjaga RTH eksisting dan melakukan revitalisasi pada lahan kosong, pemerintah juga harus memperhatikan kualitas RTH yang ada agar dapat meningkatkan kelestarian lingkungan dan kualitas hidup di Kota Tangerang., Tangerang City is an area in Banten Province that is experiencing land use change due to the increasing population. In the midst of these problems, green city has emerged as a concept of sustainable urban development. In line with the development of Tangerang City, the government's efforts in realizing a green city need to be analyzed to determine the city's performance. The main objective of this study is to evaluate the implementation of the green city concept in Tangerang City. At a macro level, the evaluation is conducted by analyzing land cover change using remote sensing. On a micro level, the quality of green open space was also analyzed by identifying selected public green spaces based on the criteria of 8 green city attributes. The results showed that the green open space area in Tangerang City continued to decline from 2009 by 38.29%, then in 2016 by 28.07%, to 2023 by 21.84%. On the other hand, the percentage of implementation of green city attributes in Alun-alun Cibodas and Hutan Kota Tangerang is 48.30% and 57.28% respectively, indicating that the quality of the two green spaces is still poor. In addition to maintaining existing green spaces and revitalizing vacant land, the government must also pay attention to the quality of existing green spaces in order to improve environmental sustainability and quality of life in Tangerang City.
Keyword: Remote sensing, green city, green open space, land cover change

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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