Judul: Kepastian Spesies Macrobrachium sintangense (de Man, 1898) Menggunakan Gen Parsial COI dari Perairan Danau Lido, Jawa Barat
Abstrak: Macrobrachium sintangense dengan nama lokal udang regang pernah diinformasikan bahwa tidak ditemukan pada periode Desember 2015-Mei 2017 di perairan Danau Lido, Jawa Barat. Oleh karena itu, kepastian spesies udang yang teridentifikasi secara morfologi sebagai M. sintangense dibutuhkan untuk memperbaiki pengelolaan sumberdaya. Penelitian ini bertujuan untuk mengidentifikasi dan memastikan spesies udang regang menggunakan pendekatan molekuler dengan marka gen parsial COI. DNA total diisolasi dari jaringan sampel menggunakan kit komersil (Geneaid). Tiga DNA sampel berkualitas baik diperoleh pada tahap amplifikasi untuk disequencing. Hasil sequencing disejajarkan menggunakan MEGA X. Hasil BLASTn diperoleh bahwa sekuen sampel lebih mirip dengan sekuen Macrobrachium sp. AB235301.1 sebesar 95% dengan 26 situs basa nukleotida spesifik. Sementara itu, sekuen M. sintangense MF622027.1 ditemukan pada database gen COI. Analisis filogeni menunjukkan bahwa sekuen sampel berada pada posisi clade terpisah dengan sekuen database M. sintangense. Sampel udang yang teridentifikasi secara morfologi sebagai M. sintangense belum tervalidasi dengan pendekatan molekuler dengan marka gen parsial COI sebagai spesies yang sama., Macrobrachium sintangense with the local name regang shrimp has been informed that it was not found in the period December 2015-May 2017 in the Lake Lido waters, West Java. Therefore, certainty of the species of shrimp identified morphologically as M. sintangense is needed to improve resource management. This study was aimed to identify and confirm the species of regang shrimp using a molecular approach with COI partial gene markers. Total DNA was isolated from the tissue sample using commercial kit (Geneaid). Three good quality DNA samples were obtained at the amplification stage for sequencing. Sequencing results were aligned using MEGA X. The BLASTn results showed that the sample sequence most similar to the Macrobrachium sp. sequence by 95% with 26 specific nucleotides. Meanwhile, M. sintangense MF622027.1 sequence was found in the COI gene database. Phylogeny analysis showed that the samples sequences were in a separate clade position with the M. sintangense database sequence. Shrimp samples identified morphologically as M. sintangense have not been validated by a molecular approach with the COI partial gene markers as the same species.
Keyword: COI gene, nucleotide base, regang shrimp

Judul: The Diversity of Freshwater Shrimp in East Kalimantan
Abstrak: Udang air tawar tersebar luas di Indonesia, termasuk dalam anggota dari subfilum Crustacea dan ordo Decapoda. Udang air tawar yang banyak ditemukan di Indonesia adalah famili Atyidae dan Palaemonidae. Anggota famili Atyidae yang umumnya ditemukan dari genus Caridina, sedangkan anggota famili Palaemonidae yang umumnya ditemukan dari genus Macrobrachium. Informasi mengenai keanekaragaman udang air tawar di Kalimantan Timur belum dilaporkan sedangkan, kualitas perairan di Kalimantan Timur semakin menurun seiring dengan meningkatnya kegiatan ekploitasi sumber daya alam. Penelitian ini akan dimanfaatkan sebagai informasi dasar keanekaragaman jenis udang air tawar untuk masa depan sebelum terjadi perubahan lebih lanjut di wilayah Kalimantan Timur. Metode yang digunakan untuk identifikasi spesies dilakukan dengan mengunakan karakter morfologi dan diverifikasi menggunakan DNA barcoding. Pengamatan karakter morfologi dilakukan dengan kamera dan juga mikroskop stereo. Hasil pengamatan karakter morfologi yang ditemukan kemudian dibandingkan dengan kunci identifikasi dan deskripsi spesies udang air tawar. Analisis DNA barcoding dilakukan dengan tahapan ekstraksi, amplifikasi gen COI, elektroforesis, sekuensing DNA, dan pengolahan data menggunakan MEGA 11 dan mrBayes. Proses analisis data keragaman genetik gen COI antar populasi udang air tawar dilakukan dengan menggunakan DNAsp Penelitian ini melaporkan catatan Keanekaragaman Udang Air Tawar di Kalimantan Timur yaitu terdapat 11 spesies udang air tawar telah terkonfirmasi, famili Palaemonidae berjumlah tujuh spesies yaitu Marobrachium rosenbergii, M. lanchesteri, M. Scabriculum, M. mammillodactylus, M. sintangense, M. equidens dan M. villosimanus, sedangkan famili Atyidae berjumlah 4 spesies yaitu Caridina typus, C. peninsularis, C. gracilipes dan C. propinqua dari famili Palaemonidae. Serta ditemukan distribusi pada spesies M. sintangense di Kalimantan Timur dengan wilayah lain yaitu Jawa merupakan populasi yang berbeda. Populasi M. sintangense di Kalimantan Timur dan Thailand diketahui memiliki keterhubungan genetik, sementara berbeda secara genetik dari populasi di Jawa., Freshwater shrimps are widely distributed in Indonesia, belonging to the subphylum Crustacea and the order Decapoda. The most commonly found freshwater shrimps in Indonesia are from the families Atyidae and Palaemonidae. Members of the family Atyidae are generally found in the genus Caridina, while members of the family Palaemonidae are commonly found in the genus Macrobrachium. Information on the diversity of freshwater shrimps in East Kalimantan has not yet been reported, while the water quality in East Kalimantan continues to deteriorate due to increasing natural resource exploitation activities. This research will serve as baseline information on the diversity of freshwater shrimp species for the future before further changes occur in the East Kalimantan region. The method used for species identification involves morphological character analysis and verification using DNA barcoding. Morphological character observations were conducted with the help of cameras and stereo microscopes. The observed morphological characters were then compared with identification keys and descriptions of freshwater shrimp species. DNA barcoding analysis was carried out through the steps of extraction, COI gene amplification, electrophoresis, DNA sequencing, and data processing using MEGA 11 and MrBayes. Genetic diversity analysis of the COI gene among freshwater shrimp populations was performed using DNAsp. This study reports on the diversity of freshwater shrimp in East Kalimantan, confirming the presence of 11 species. The family Palaemonidae is represented by seven species: Marobrachium rosenbergii, M. lanchesteri, M. scabriculum, M. mammillodactylus, M. sintangense,
Keyword: Caridina, COI, distribution, genetic distance, Marobrachium

Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4