Judul: Isolasi Bakteri Endofit dan Penguj ian Potensinya untuk Mengendalikan Nematoda Meloidogyne spp. pada Tanaman Tomat (Lycopersicon esculentum Mill.)
Abstrak: Penelitian ini bertujuan untuk mengisolasi dan menguji bakteri endofit yang berasal dari tanaman tomat dan cabai untuk mengendalikan nematoda Meloidogyne spp. serta mengetahui pengaruhnya terhadap pertumbuhan tanaman. Bakteri endofit diisolasi dari pertanaman tomat dan cabai yang berasal dari daerah Bogor dan Malang. Isolasi bakteri endofit dengan menggunakan metode sterilisasi permukaan dan homogenisasi jaringan. Sterilisasi permukaan jaringan menggunakan ethanol 80 % dan NaOCl 3 % dan 1,5 %. Bakteri yang didapat selanjutnya dimurnikan dan disimpan dalam media Trytic Soy Broth (TSB) yang dicampur dengan glycerin 20 % pada suhu -79°C sebagai stok. Pengujian bakteri endofit dilakukan secara in vitro dan in vivo. Pengujian in vitro bertujuan untuk melihat pengaruh kultur filtrat bakteri endofit terhadap ketahanan hidup larva Meloidogyne spp., sedangkan uji in vivo bertujuan untuk melihat pengaruh sel bakteri yang diinokulasikan pada tanaman terhadap pertumbuhan tanaman dan pembentukan puru akar yang disebabkan oleh Meloidogyne spp. pada tanaman tomato Uji in vivo dilakukan dirumah kaca Institut Pertanian Bogor dengan metode perlakuan tanah dan perlakuan benih. Hasil uji in vitro menunjukkan bahwa perlakuan isolat bakteri endofit berpengaruh terhadap tingkat ketahanan hidup larva Meloidogyne spp.. Hasil uji di rumah kaca menunjukkan bahwa perlakuan bakteri endofit pada tanaman tomat dapat menekan pembentukan puru akar yang disebabkan oleh Meloidogyne spp. Identifikasi isolat bakteri uji dilakukan dengan melihat karakteristik koloni bakteri dan dengan beberapa pengujian yaitu uji gram (KOH 3 %), pewarnaan gram, uji bakteri tahan panas dan uji flourescent. Hasil identifikasi menunjukkan isolat bakteri uji yang digunakan adalah dari genus Bacillus sp. dan bakteri Pseudomonas flourescent.
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Judul: Isolasi Bakteri Endofit dari Tanaman Kehutanan dan Potensinya untuk Pengendalian Meloidogyne spp. pada Tanaman Tomat
Abstrak: Meloidogyne spp. is one of the main constraints of tomato production in Indonesia. Endophytic bacteria as a biocontrol agents is an alternative for controlling nematodes. The objective of this study was to isolate endophytic bacteria from forest plants and to evaluate its potential for controlling Meloidogyne spp. on tomato (Lycopersicon esculentum Mill.). Endophytic bacteria were isolated from mahoni (Swietenia mahogany Jacq), trambesi (Albizia saman (Jacq.) Merr.), gaharu (Aquilaria malacensis), and meranti (Shorea sp.) with surface sterilization method using 70% alcohol, 4% NaOCl and steril water. The bacterial strain were cultured on tryptic soy agar (TSA) for two days. In vivo testing to determine the effect of bacteria for suppressing of nematode galls was conducted in the greenhouse with seed treatment. The result showed there are thirty three isolates of endophytic bacteria were isolated from the root of forest plant. twenty two isolates are negative reaction and eleven isolates are positif reaction after hypersensitive test using tobacco plant. Two isolates of endophytic bacteria MSJ1H and TSS1D were able to reduce gall number. Based on high plant indicator, soaking seed treatment with endophytic bacteria were able to increase the high of tomato plants.
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Judul: Dampak Perceraian dan Hubungan Sosial terhadap Kesejahteraan Psikologis Ibu sebagai Orang Tua Tunggal
Abstrak: Family with a single mothers who came from divorced, often bring the impact such as problem or change on a single mothers well-being. The aim of this research to indentify characteristics of single mother and relations with psychological well-being and social relations, and also analyze impact divorce on psychological well-being and social relations. Descriptive analysis, regression, and Pearson correlation was used in this study. The respondents were sixty single mother who come from the length divorced more than six month. The result showed that compare with present condition, psychological well-being, impact of divorce and social relation respondent are more significant lower than the first six month after divorce. There was relationship between age of present and divorce, family’s income, and size of family with level of depression, social support, and economic impact. Based on regression analysis we found there was negative effect of impact of divorce to psychological well-being at the first six month after divorce and the present. Other wise, we found there was not significant effect of divorce to social relationship.
Keyword: economic impact, level of depression, social support