Judul: Pemanfaatan ruang terbuka untuk permainan-anak tradisional di Desa Baginda, Kecamatan Sumedang Selatan, Kabupaten Sumedang
Abstrak: Ruang terbuka di Kabupaten Sumedang mengalami penurunan luasan. Padahal, salah satu fungsi dari ruang terbuka ialah sebagai ruang bermain anakanak. Tujuan penelitian ini ialah mengkaji seberapa besar pemanfaatan ruang terbuka untuk permainan-anak tradisional di Desa Baginda dan mencari kebutuhan luasan untuk jenis permainan tradisional tersebut. Pengumpulan data dilakukan dengan penyebaran kuisioner kepada 30 responden anak-anak dan 30 responden orangtua. Kuisioner tahap satu disebar untuk mencari informasi mengenai persepsi masyarakat mengenai kegiatan bermain di ruang terbuka, khususnya untuk permainan tradisional. Selain mencari persepsi, dicari pula preferensi mengenai jenis permainan tradisional yang diketahui dan masih digemari oleh masyarakat di Desa Baginda. Kuisioner tahap dua mencari informasi mengenai kebutuhan luasan untuk masing-masing permainan yang dihasilkan dari penyebaran kuisioner tahap satu. Hasil yang diperoleh menunjukkan bahwa masyarakat desa Baginda memiliki pengetahuan mengenai permainan tradisional Sunda dan memiliki keinginan untuk bermain permainan tersebut di ruang terbuka. Namun, terdapat beberapa faktor penghambat, seperti kurangnya data atau informasi mengenai permainan tradisional, tidak adanya pihak yang mengajarkan, dan tidak adanya ruang bermain anak yang dibuat secara khusus oleh pihak desa. Ruang terbuka yang biasanya digunakan untuk bermain anak-anak berupa lapangan, sawah, dan lahan kosong. Di Desa Baginda terdapat ruang terbuka berupa lapangan sepak bola yang dapat dimanfaatkan untuk kegiatan pameran atau lomba permainan tradisional. Kedua kegiatan tersebut dapat dilakukan pada peringatan hari-hari besar dan tidak bersifat permanen. Hal tersebut mempertimbangkan fungsi utama lapangan ruang terbuka yang ada untuk berolahraga. Selain itu, hasil yang diperoleh menunjukkan bahwa masyarakat desa Baginda tidak mengenali seluruh jenis permainan tradisional Sunda yang diakui oleh Dinas Pariwisata dan Kebudayaan Jawa Barat. Terdapat 16 jenis permainan tradisiona Sunda yang dikenal dan masih digemari hingga saat ini di Desa Baginda. Setelah memperoleh data jenis permainannya, dilakukan pencarian informasi mengenai kebutuhan ruang untuk bermain permainan tersebut dengan menyebar kuisioner tahap dua kepada responden yang sama. Hasilnya, lahan yang dibutuhkan untuk mengakomodasi 16 jenis permainan tersebut adalah 607 meter². Permainan tersebut adalah bebeletokan (30 m²), bedil jepret (35 m²), bekel (6 m²), congklak (6 m²), egrang/jajangkungan (53 m²), galah asin (126 m²), hong 25/ucing peungpeun/ucing sumput (63 m²), ketepel/bandring (67 m²), lompat karet (25 m²), ngadu karbit (51 m²), ngadu muncang (11 m²), oray-orayan (28 m²), sapintrong (21 m²), sondah/engklek (16 m²), sur-ser (7 m²), dan susumputan (62 m²). Hasil akhir dari penelitian ini berupa rekomendasi desain ruang terbuka dengan fasilitas permainan tradisional Sunda.
Keyword: ruang bermain anak, ruang terbuka, permainan tradisional

Judul: Rencana Pengelolaan Lanskap Pertanian Desa Pangulah Utara, Kecamatan Kota Baru, Kabupaten Karawang, Jawa Barat, sebagai Sarana Bermain Anak-Anak
Abstrak: Play is a need for children to learn how to socialize and develop their creativities. Agricultural landscape of Desa Pangulah Utara has a potential that could be developed as a children playground facility. The open space can be exploited as a mean to support play activities. The purpose of this research is to create a management plan of agricultural landscape of Desa Pangulah Utara, Kecamatan Kota Baru, Kabupaten Karawang, Jawa Barat, as a means of children playing and helps children to learn how to socialize and interact with their environment, also to introduce more about Sundanese traditional games. Data that used in this research consists of physical, biophysical, social, and managerial data. These data were analyzed descriptively, statistically, and by means of SWOT method. The results of SWOT analysis are six recomendation of management strategies of Desa Pangulah Utara as a mean of children playground: (1) providing the facilities that supporting children to play especially to play Sundanese traditional games, (2) providing play areas, (3) making the playing program that can increase the interest of children about Sundanese traditional games, (4) using local vegetation as materials of Sundanese traditional games, (5) making landscape management plan of agricultural landscape of Desa Pangulah Utara as children playground, and (6) specifying the areas that used as childreen playground without changing its function.
Keyword: traditional games, SWOT analysis, playing, management, agricultural landscape

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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