Judul: Pengembangan Ekowisata di Kawasan Trianggulasi Taman Nasional Alas Purwo.
Abstrak: Trianggulasi placed in Resort Rowobendo, Alas Purwo National Park. Trianggulasi attraction of ecotourism are diverse, due to the manager could not utilize and manage optimally, it became unmaintained. The main purpose of this research was to formulated development strategy of ecotourism in Trianggulasi based aspects of demand and aspects of supply. The research was conducted with data collection methods such as observation, questionnaires, interviews and literature. Development strategy of ecotourism in Triangulasi work by creating ecotourism products on visitors demand, encouraging parties to immediately operationalize WWAH business space that has been leased, community empowerment, repairing facilities and infrastructure, optimization of marketing and promotion on target market, provide advices to minimize the impact of environmental damage caused by ecotourism, arrangement for visitors and improving the quality of human resources.
Keyword: Alas Purwo national park, ecotourism, development strategy, trianggulasi

Judul: Pengembangan Ekowisata Hutan Mangrove Teluk Pangpang, Taman Nasional Alas Purwo.
Abstrak: Taman Nasional Alas Purwo (TNAP) memiliki beberapa areal mangrove, salah satunya adalah hutan mangrove Teluk Pangpang Resort Kucur. Hutan mangrove dapat dijadikan sebagai objek ekowisata. Ekowisata mangrove dapat dikembangkan berdasarkan ekologi dan daya dukung kawasannya. Pentingnya pengembangan ekowisata mangrove agar semua sumberdaya yang ada dapat dimanfaatkan dengan maksimal tanpa merusak sumberdaya. Tujuan dari penelitian ini adalah mengidentifikasi sumberdaya alam yang dapat dijadikan potensi ekowisata, pengelolaan ekowisata, motivasi, persepsi dan preferensi pengunjung, serta menyusun rekomendasi pengembangan. Penelitian ini dilakukan di hutan mangrove Teluk Pangpang TNAP dan Desa Kedungasri pada bulan Maret – April 2020. Pengambilan data dilakukan dengan cara menyebar kuesioner, wawancara, observasi lapang, studi pustaka. Sumberdaya mangrove yang dapat dikembangkan menjadi objek ekowisata berdasarakan aspek fauna, flora dan sosial budaya adalah Egretta garzetta, Pavo muticus, Sonneratia alba berukuran besar, bentang alam mangrove, jenis-jenis kerang, ikan belanak serta budaya Takir sewu dan kerajinan layang-layang. Pengelolaan ekowisata hutan mangrove Teluk Pangpang TNAP hanya dilakukan oleh TNAP saja tanpa melibatkan pihak lain diluar kawasan TNAP. Motivasi pengunjung untuk datang ke ekowisata paling besar adalah untuk memperluas wawasan, persepsi paling besar terhadap fasilitas adalah tempat informasi dan terhdap pengelola paling besar perencanaan objek wisata, untuk preferensi yang dipilih oleh pengunjung adalah mengambil gambar, menyaksikan tarian daerah dan menikmati kuliner laut. Strategi pengembangan yang dilakukan adalah strategi SO (Strength - Opportunity) dengan pemberdayaan masyarakat dalam menjalankan kegiatan ekowisata dan juga menambahkan objek ekowisata berdasarkan minat pengunjung.
Keyword: ekowisata, hutan mangrove Teluk Pangpang, Taman Nasional Alas Purwo

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
Keyword: