Judul: Aktivitas bakteri pengoksidasi amonium isolat ASR1 dan ASR2 asal tambak udang pada sumber karbon dan salinitas yang berbeda
Abstrak: Proses oksidasi amonium atau nitrifikasi merupakan salah satu proses yang dapat digunakan dalam usaha perbaikan kualitas air tambak udang melalui pendekatan biokondisioner. Proses ini dapat dilakukan pada kondisi autotrof dan heterotrof. Kedua kondisi tersebut dibedakan berdasarkan substrat yang digunakan oleh bakteri sebagai sumber karbonnya. Habitat estuaria seperti tambak udang memiliki kondisi salinitas yang berfluktuasi, hal ini membuat aktivitas bakteri nitrifikasi dipengaruhi oleh tingkat salinitas. Hasil pengamatan menunjukkan bahwa isolat ASR1 dan ASR2 dapat mengoksidasi amonium pada kondisi autotrof dengan karbonat sebagai sumber karbon dan pada kondisi heterotrof dengan glukosa, gliserol, asetat dan suksinat sebagai sumber karbon. Penurunan konsentrasi amonium yang terjadi disebabkan oleh penggunaan hasil senyawa oksidasi amonium sebagai sumber nitrogen dan sumber energi. Isolat ASR1 menunjukkan bahwa aktivitas oksidasi tertinggi adalah pada media dengan gliserol sebagai sumber karbon dengan persentase amonium yang dioksidasinya sebesar 83 %. Isolat ASR 2 menunjukkan bahwa aktivitas oksidasi amonium tertinggi adalah pada media dengan suksinat sebagai sumber karbon dengan persentase amonium yang dioksidasinya sebesar 98 %. Hasil pengamatan pada uji selanjutnya yaitu uji optimasi salinitas dengan salinitas 0, 5, 10, 15 dan 20 ppt yang menggunakan isolat ASR2 dengan suksinat sebagai sumber karbon memperlihatkan bahwa tingkat salinitas sebesar 20 ppt adalah yang tertinggi dalam tingkat salinitas uji untuk proses oksidasi amonium.
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Judul: Aktivitas oksidasi amonium dan reduksi Nitrat Alcaligenes sp. Isolat SDTT5 dan KDTST3 asal tambak udang menggunakan berbagai sumber karbon
Abstrak: Water quality is one problem found in shrimp culture caused by increasing of ammonium and nitrite concentration. This problem correlates with a~mulation of uneaten feed and shrimp waste products. Both ammonium and dhUte are toxic to shrimp. Recently, addition of bacteria as bioremidiation agent to;:shrimp ponds can remedy water quality. SDTT5 and KDTST3 isolate are ni:trate reducing bacteria isolated from shrimp ponds using denitrification media ~h acetate as carbon source in anaerob condition. Based on mollecular analysis 0~1 6S-rRNA sequences the isolate was 99% closely related to Alcaligenes sp. TIDs study was to investigate ammonium oxidation and nitrate reduction activities bis DTT5 and KDTST3 isolates on different carbon sources. This expriment was c<j.ducted used different media i.e. heterotroph and autotroph nitrification media, ami ammonium oxidation and nitrate reduction media. The incubation was held u~er either aerob or anaerob conditions. The results shows that SDTT5 and Kf>TST3 isolates were able to oxidize ammonium heterotrophycally with acetate, s~cinate, glucose and glycerol as carbon sources and autothrophically with cibonate as carbon source. The highest activity of ammonium oxidation by St>TT5 and KDTST3 was found in succinate and acetate as carbon sources. S~TT5 isolate was able to oxidize ammonium and reduce nitrate either aerobically or anaerobically with different carbon sources. Under anaerob c.gndition SDTT5 isolate was able to oxidize ammonium and reduce nitrate with a~tate, succinate, glucose and glycerol as carbon sources but SDTT5 isolate can not oxidize ammonium and reduce nitrate aerobically with glycerol as carbon source Meanwhile, KDTST3 isolate was only able to oxidize ammonium aerobically with acetate, succinate, and glucose as carbon sources, and reduced nitrate with glycerol as carbon source.
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Judul: Etno-ornitologi Burung Kancilan Flores (Pachycephala nudigula nudigula Hartert 1897) pada Masyarakat Lio di Taman Nasional Kelimutu
Abstrak: Bare-throathed whistler is one of several wild birds in Kelimutu National Park (TNKL) that is believed to have correlation with Lio Community‟s belief. The estimation of Bare-throated whistler‟s population in Kelimutu National Park was about 186.41-1668.07 individual by the density about 0.0389-0.3481 ind/ha with the sex ratio 6.2:1 that spreaded on area with height of 1100 – 1600 masl. The characteristic of Bare-throated whistler‟s habitat is a natural forest with various canopy level, high plant species diversity and high density. The habitat characteristics related to the availability of food in the form of insects, cover, safety and a nesting site. Generally the most of Lio community knew the existence of Bare-throated whistler directly or from their parents‟ stories. The perceptions of Lio Community to the Bare-throated whistler‟s existence is generally support the conservation efforts. The perceptions construct the Lio Community‟s atitude that tend to be conservative to the existence of Bare-throated whistler. The attitude shown by their behavior that not cathing the Bare-throated whistler from the TNKL‟s area or the outside. The attitude have an important role to the existence of Bare-throated whistler in Kelimutu National Park‟s area.
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