Judul: Water Footprint Analysis of Pocket Fertigation System for Cultivating Horticultural Plants in Open Land
Abstrak: Masalah efisiensi air masih menjadi isu penting dalam pertanian yang dapat direpresentasikan dengan indikator water footprint. Penghematan air dapat dilakukan dengan pengaplikasin sistem irigasi bawah permukaan, salah satunya sistem pocket fertigation. Tujuan penelitian ini menganalisis water footprint pada sistem pocket fertigation dengan berbagai skenario. Penelitian ini dilakukan di Kinjiro Farm pada waktu Maret – Juni 2023. Keempat skenario meliputi IK1 yang merupakan irigasi konvensional dengan irigasi satu kali evaporasi, IK2 yang merupakan irigasi konvensional dengan irigasi dua kali evaporasi, PF1 yang merupakan pocket fertigation dengan irigasi satu kali evaporasi, dan PF2 yang merupakan pocket fertigation dengan irigasi dua kali evaporasi, Hasil panen terbaik ditunjukkan PF1 dengan rata-rata produksi 13,38 ton/ha. Nilai water footprint terendah terjadi pada PF1 sebesar 540,37 m3/ton. Skenario PF2, IK1, dan IK2 memiliki water footprint berturut-turut 24%, 27%, dan 51% lebih besar dari water footprint PF1. PF1 menjadi skenario terbaik dikarenakan pemberian air yang langsung menuju perakaran dan jumlah irigasi mendekati kebutuhan air tanaman.
Keyword: evaporation, conventional irrigation, production, pocket fertigation, water footprint

Judul: Water Footprint Analysis on Melon Cultivation in Greenhouse Using Pocket Fertigation Subsurface Irrigation System
Abstrak: Potensi sumber daya air Indonesia mencapai 3,9 triliun m3/tahun, tetapi sekitar 3,2 triliun m3/tahun belum dimanfaatkan. Ketersediaan air irigasi berpengaruh pada pertumbuhan dan produksi tanaman. Budidaya tanaman melon cukup sulit dikarenakan kecocokan pada kondisi tanah dan udara di lingkungan pertumbuhan tanaman. Salah satu teknik irigasi yang dapat digunakan, yaitu pocket fertigation. Penelitian ini bertujuan menganalisis water footprint tanaman melon dalam greenhouse dengan sistem irigasi pocket fertigation dan konvensional, serta menentukan sistem irigasi paling optimal. Penelitian dilakukan bulan Maret-Juli 2022 di Kinjiro Farm, Kota Bogor. Tahapan penelitian meliputi persiapan lahan, budidaya tanaman melon, pengumpulan data, dan pengolahan data. Rancangan penelitian terdiri dari sistem irigasi pocket fertigation (PF) dan konvensional (SK), serta pemberian air satu kali (E) dan dua kali evaporasi (2E). Sistem irigasi PF-2E menjadi tanaman tertinggi dengan jumlah daun lebih banyak dibandingkan metode lainnya. Sistem irigasi PF-2E lebih efisien untuk diaplikasikan pada tanaman melon, dikarenakan memiliki hasil panen dan produktivitas air yang lebih tinggi, serta water footprint yang lebih rendah dibandingkan metode konvensional., The potential of Indonesia's water resource reaches 3,9 trillion m2/year, but around 3,2 trillion m2/year has not been utilized. Air irrigation affects plant growth and production. Melon plant cultivation is quite difficult because of its suitability to the soil and air conditions in the environment. One of the irrigation techniques is pocket fertigation. This research aims to analyze the water footprint of melon plants in a greenhouse with pocket fertigation and conventional irrigation systems, and determine the most optimal irrigation system. The research was conducted in March-July 2022 at Kinjiro Farm, Bogor City. The research stages include land preparation, melon cultivation, data collection, and processing. The research design consists of pocket fertigation (PF) and conventional irrigation systems (SK), as well as one time (E) and two times evaporations of water (2E). PF-2E irrigation system is the tallest crop with more leaves than other methods. PF-2E irrigation systems are more efficient to be applied to melon plants, because it has higher yields and water productivity, and lower water footprint than conventional methods.
Keyword: evaporation, irrigation, melons, pocket fertigation, water footprint

Judul: Mitochondrial DNA Gene Organization and Phylogenetic Relationship of Naja siamensis
Abstrak: Naja siamensis is one of Asiatic cobra in the Family of Elapidae, known as a cryptic snake. It has many morphological variations that lead to misidentification. Misidentification can cause the wrong concept in developing antivenom types due to high snakebite cases occur in Thailand. Thus, an accurate species identification using a molecular approach is crucially required. Mitochondrial DNA has been used widely in snake molecular studies. These studies were aimed to determine the arrangement of the N. siamensis partial mitochondrial genes using reptile-oriented primers and to reveal the phylogenetic relationship among other species of Asian cobra. DNA extraction was carried out based on the standard DNA salting-out protocol and was conducted by bioinformatics analysis using BLASTn and MEGA 6 software. Gene annotation was performed against N. kaouthia complete genome. A total of 3121 bp of N. siamensis mtDNA genes were sequenced consisting of partial of six regions: 16S rRNA, ND1, tRNA-Ile, CRII region, tRNA-Leu, tRNA-Gln, and COI genes. The mtDNA gene organization of N. siamensis was predicted to has a similar pattern with the common Elapidae mtDNA gene organization. This study showed that there is a monophyletic form in Naja genus and N. kaouthia is the closest species with N. siamensis based on partial 16S rRNA and ND1 gene.
Keyword: 16S rRNA, Elapidae, ND1, phylogenetic relationship