Judul: Kajian Histopatologi Pengaruh lmunisasi Intraperitoneal Streptococcus agalactiae Hemaglutinin Positif Pada Mencit Bunting
Abstrak: Kajian Histopatologis terhadap jaringan ambing mencit bunting yang diimunisasi S. agalactiae hemaglutinin positif dilakukan pada 6 ekor mencit balb-C yang dibagi l11enjadi 3 kelompok yaitu kelompok kontral negatif, kontral positif dan kelompok yang diimunisasi. Mencit-l11encit tersebut diimunisasi dengan suspensi bakteri utuh s.agalacliae hemaglutinin positif sebanyak 0,1 mllekor mencit melalui rute intraperitoneal. Empat hari setelah dilakukan uji tantang dengan bakteri yang sama, ambing mencit dikoleksi dan diperiksa secm'a histopatologis. Hasil pengamatan menunjukkan bahwa imunisasi tidak memberikan perlindungan terhadap uji tantang yang dilakukan. Kelenjar ambing mencit l11engalami peradangan yang ditunjukkan dengan adanya infiltrasi sel radang, epitel alveol l11engalal11i degenerasi hingga nekrosa. menyebabkan struktur alveol l11enghilang dan teljadi pembentukanjaringan lemak (jal pad) lebih dini. Uji statistik menunjukkan bahwa pembentukan jaringan ikat. jaringan lemak dan sel radang l11ennnjukkan perbedaan nyata (P<0,05) pada kontral positif, kelompok perlakuan dengan kontral negatif.
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Judul: Kajian Histopatologis Pengaruh Vaksin Streptococcus agalactiae yang Diradiasi pada Organ Hati dan Ginjal Mencit (Mus musculus)
Abstrak: This research was conducted to study the effect of irradiated vaccine Streptococcus agalactiae as attenuated vaccine (LD50) by gamma iradiation for the prevention of subclinical mastitis. The study was carried out on the liver and kidney of mice (Mus musculus). Twelve female mice were used in this study which divided into 4 groups, negative control (K); positive control (T); vaccine group (V); vaccine and challange group (VT). The treatment were done during pregnancy and challange post parturient (7 days), while vaccination gave during pregnancy with interval one week between first vaccination and boosting vaccine with 108cfu/ml/mice of S. agalactiae suspension a long four weeks. A week after parturient, mice were challange by S. agalactiae isolated from bovine subclinical mastitis case, dropped 50μl 108cfu/ml/teat on to orificium externa of mice mammary teat. One day after word followed by necropsy, samples were collected and fixed by Buffered Neutral Formalin 10% (BNF 10%) and procecced for histopathological slides. The parameters studied were counting of hepatocytes changes as degeneration, inflammation and necrosis both in central and portal vein region. These parameters also observed in kidney on glomerular and tubular part. Data were analized by ANOVA test and followed by Duncan test. The result showed that irradiated vaccine S. agalactiae did not have toxic effect on liver and kidney of mice that can be proved with have no significant different (p<0,05) compared between V group and K group include increase of degeneration, inflamatory cell, and cell death.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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