Judul: Deteksi Bakteri Salmonella spp. pada Berbagai Produk Sushi Komersial dan Eksplorasi Kandidat Biosensor DNA.
Abstrak: Keracunan makanan dapat disebabkan oleh berbagai faktor, salah satunya kontaminasi bakteri patogen yaitu Salmonella spp. Metode berbasis molekuler membutuhkan waktu yang cepat, lebih sensitif, dan spesifik. Tujuan penelitian ini adalah mengaplikasikan metode berbasis DNA molekuler untuk mendeteksi bakteri Salmonella spp. pada produk sushi komersial dan merancang serta mengidentifikasi oligonukleotida spesifik dari Salmonella spp. Primer yang digunakan untuk gen invA Salmonella spp. memiliki panjang amplifikasi 941 bp. Terdapat dua sampel produk sushi komersial yang mengandung Salmonella spp. Sampel yang positif mengandung Salmonella spp. yaitu sampel S2.1 dan S2.3. Primer spesifik Salmonella spp. dirancang dalam penelitian ini untuk menciptakan suatu primer spesifik dan berukuran lebih kecil yang diharapkan efisien untuk digunakan sebagai marka deteksi dengan PCR atau sebagai bioreseptor pada kit nukleosensor.
Keyword: DNA molekuler, invA, kontaminasi, nukleosensor, Salmonella, sushi

Judul: Aplikasi Real-Time Pcr Untuk Mendeteksi Bakteri Salmonella Sp. Pada Hasil Perikanan
Abstrak: Salmonella termasuk ke dalam grup bakteri patogen yang menyebabkan foodborne ilness. Penyakit yang disebabkan oleh Salmonella diantara nya adalah gastrodermik, demam tipoid dan dapat menyebabkan kematian. Penelitian ini menggunakan sampel hasil perikanan segar dan olahan yang diperoleh dari pasar tradisional dan pasar modern di Bogor. Tujuan penelitian ini mengaplikasikan real- time PCR untuk mendeteksi bakteri Salmonella sp. pada hasil perikanan. Primer yang digunakan untuk mendeteksi gen target bakteri Salmonella sp.. memiliki panjang amplifikasi 204 bp. Hasil absorbansi dengan rasio 260/280 pada selang 1,6 dan 2,0. Sampel hasil perikanan yang berasal dari pasar tradisional dan pasar modern baik segar dan olahan tidak ditemukan produk yang terkontaminasi bakteri Salmonella, hal ini karena sampel tidak mencapai nilai Threshold Cycle (Ct), tidak mencapai nilai dari melting curve kontrol positif dan tidak menunjukan grafik yang sigmoid.
Keyword: kontaminasi, produk perikanan, real-time PCR, Salmonella

Judul: Thermal Characteristics and Dynamics of Tropical Cyclone over Western North Pacific Ocean: A Case Study of Typhoon Songda (Chedeng) 2011
Abstrak: The frequent occasion of Tropical Cyclone (TC) over Western North Pacific (WNP) Ocean lies from 10˚N to 26˚N and 121˚E to 170˚E. Sources of TC development come from warm ocean temperature where latent heat is released during condensation processes and subsequently concentrated in the boundary layer. Relative humidity at 850-700 hPa is responsible to produce the most intense storm. Meanwhile, weak vertical wind shear between 850-200 hPa is one of the dynamical factors that lead to the development of TC. Pressure level of 850-200 hPa is chosen to minimize the thermodynamic effect in the lower troposphere. Typhoon Songda (Chedeng in the Philippines) or TSC lasted from May 18th to 30th, 2011 and strengthened into powerful Super Typhoon stage between May 24th and 27th. Long curving track and the exhibition of all sequences of TC life cycle, TSC is then considered as an ideal case of TC. The objective of the study with case of TSC is to investigate the role of thermal characteristics and dynamics on TC intensity. The research is carried out by using Advanced Research of Weather Research and Forecasting model (WRF ARW model) version 3.3. Two domains are employed in the model. The first domain situate from 02˚N to 40˚N and 118˚E to 149˚E and cover spatial resolution () of 33.1 km. The second domain situate from 09˚N to 27˚N and 119˚E to 132˚E with of 11 km. Timestep of the model () is 120 seconds. The model is supported by the following physical parameterization, i.e., Yonsei University Scheme (YSU) in the boundary layer, Kain-Fritsch scheme (KF) in the cumulus, and WRF Single-Moment 3-class scheme in the microphysics. Initial meteorological condition prior to the formation of the cyclone is generated using WRF ARW standard-release model. Sensitivity of the model output is obtained from a selected single-parameter change scenarios, namely, 10 % to 20 % decrease and increase of wind shear initial data at 850-200 hPa, and by 10 % to 20 % decrease of relative humidity initial data at 850-700 hPa. The result showed that the negative correlations between wind shear and intensity are found in wind shear scenarios. Wind shears of 10 ms-1 which lead to strengthening of the wind intensity in the early period of Typhoon development are found in wind shear scenarios. The effect of wind shear changes on wind intensity is statistically significant in the 20 % wind shear scenarios. During mature stage, weak wind intensity occurred when wind shear increase as given in the WRF standard-release and the 20 % decreasing scenario. Different situation is found in the 20 % increasing scenario where wind shear tend to weak and lead to strong wind intensity at this stage. Meanwhile, the reduction of moisture supply at 850-700 hPa resulting from 20 % decreasing relative humidity play a major role in the weakening of wind intensity and reduces mechanical energy of the cycle process as much as 300 J kg-1.
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