Judul: Identifikasi Variasi Basa Nukleotida Gen Ketahanan Penyakit Blas Pi33 pada Galur Padi Terseleksi
Abstrak: Rice is one of the most important crops for human beings, thus increasing productivity are continually persecuted. Blast disease can reduce the rate of productivity of rice cultivation. Therefore, the forming programme of blast disease-resistant varieties needs to do effectively. One of broad-spectrum blast disease-resistant gene is Pi33. This study aims to identify the variation in the sequence of nucleotide bases of Pi33 gene in five interspesific strains which derived from Bio46 (IR64/Oryza rufipogon and CT13432). After isolated, DNA of five strains amplified used spesific primer for Pi33, G1010. Amplification result purified through Exo1SAP protocol. Labelling using fluorescent dyes done before sequencing nucleotide base using CEQ8000 instrument. The results showed that strains number 28 has introgesion of the three control its parent genome (subspecies of indica, subspecies of japonica, and O.rufipogon) while the strains number 79, 136, and 143 identical to indica genome, then number 195 strain identical to japonica genome. The result of ortholog analysis found conserved nucleotide base sequence, namely: CAGCAGCC which involved into heterotrimeric G-protein group. This protein has role as plant receptor for recognizing pathogen elicitor in interaction of rice and blast pathogen
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Judul: Resistance Test toward of 48 Lines Rice to Blast Disease (Pyricularia oryzae) Race 001
Abstrak: Blast disease, caused by Pyricularia oryzae Cav., is one of the major fungal diseases infected rice (Oryza sativa L.) in Indonesia. The aim of this research was to examine the resistance of rice from the infection of P. Oryzae race 001 and to detect the existance of Pi-ta gene in resistant lines of rice. Resistance evaluation to blast disease (Pyricularia oryzae) on Cisadane (resistant), Kencana Bali (susceptible) as control, and 48 lines of rice was carried out in the green house by using injection inoculated method with P. oryzae race 001. Rice seedling were planted on sterilize soil, and sixteen days old of rice were soaked in the fungal spore suspension (105 spores/ ml) in the dark for 24 hours, and moved to the light. Rice disease was observered on 7-9th days after infection. There were 2 step screening of blast-resistant, which screening I performed a total of three replicates and screening II performed from selected plant in screening I a total of two replicates for each line of rice, with 10 plants in each replication. The result of second screening at green house showed that 13 lines resistant to P. oryzae 001 were IPB140-F-2-1, IPB115-F-4-2-2, IPB113-F-2-2, IPB107-F-7-3, IPB140-F-5, IPB149-F-2, IPB149-F-8, Martapura, IPB149-F-4, IPB149-F-5, IPB113-F-1, IPB102-F-92-1-1, IPB107-F-5-1 with the percentage of resistance ≥ 80 %. Two pairs of DNA primers specific to the dominant indica Pi-ta gene were designed from DNA sequence in gene bank with accession number AF207842 to amplify the Pi-ta DNA fragments by polymerase chain reaction (PCR) method. The results revealed that all of 13 resistant and 2 susceptible lines (IPB107-F-18-4-2 and IPB107-F-16E-6) of rice carrying Pi-ta genes, even in Kencana Bali. The previous experiment shown that Kencana Bali does not have Pi-ta resistance gene. It is expected that Kencana Bali has deletion of Pi-ta resistance gene in the end part of exon 2. Further more, those results also indicated that Pi-ta gene did not confer resistance to race 001 of Pyricularia oryzae. Resistance to P. oryzae race 001 might be controlled by different resistant gene.
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Judul: Penggunaan Teorema Homeomorphy 2-Manifold dan Teorema Euler Poincare pada Torus 𝑇�� dan Simplicial Complex 𝐾��
Abstrak: Two dimensional topological spaces are said to be homeomorphic if they have the same topological invariant, where one of topological invariant used is an Euler characteristic. Homeomorphy 2-Manifold’s Theorem and Euler Poincare’s Theorem are used to distinguish two topological spaces. Homeomorphy 2-Manifold’s Theorem uses Euler characteristic to identify two dimensional topological spaces. Euler Poincare’s Theorem is an alternative way to find Euler characteristic with Betti number, which is topological invariant as well. The objective of this paper is to investigate the homeomorphism of torus 𝑇����� and simplicial complex 𝐾�����. Topological space torus and simplicial complex 𝐾����� have the same Euler characteristic, which is two. Based on Homeomorphy 2-Manifold’s Theorem topological space torus 𝑇����� and simplicial complex 𝐾����� is homeomorphic.
Keyword: simplicial, torus, Euler, homeomorphism, topology