Judul: Urban Forest Priority Development Based on Air Pollution Distribution and Land Temperature in Kota Jambi
Abstrak: Kota Jambi terus mengalami perkembangan dan berimplikasi pada pesatnya pembangunan. Hal tersebut dapat menyebabkan penurunan kualitas lingkungan sehingga dibutuhkan hutan kota dalam memitigasinya. Penelitian ini bertujuan untuk menentukan potensi lokasi hutan kota berdasarkan sebaran polutan udara dan suhu permukaan di Kota Jambi. Penentuan lokasi prioritas hutan kota menggunakan sebaran polutan udara dan suhu permukaan yang dikelaskan menurut kriteria lokasi prioritas, sedangkan tutupan lahan menjadi acuan dalam penentuan lokasinya. Data dikumpulkan menggunakan citra satelit Sentinel-5P berupa CO, NO₂, O₃ dan SO₂, sedangkan nilai suhu permukaan dan tutupan lahan dari citra Landsat 8. Semua data diolah di Google Earth Engine. Potensi lokasi hutan kota dikategorikan menjadi tiga kelas prioritas yaitu prioritas 1 dengan nilai polutan dan suhu permukaan tertinggi meliputi Kecamatan Danau Teluk, Jambi Timur dan Pelayangan; Prioritas 2 dengan nilai polutan dan suhu permukaan menengah meliputi Kecamatan Danau Sipin, Jelutung, Paal Merah dan Pasar Jambi; serta prioritas 3 dengan nilai polutan dan suhu permukaan terendah., Jambi City continues to prosper and has implicated to its rapid development. It can cause a decrease in urban environmental quality, hence the existence of urban forests is needed to mitigate such problems. This study aims to determine the potential locations for urban forests based on the distribution of air pollutants and surface temperature in Kota Jambi. Determination of priority locations uses distribution of air pollutants and surface temperature map that are classified according to priority location criteria, while land cover becomes a reference in determining its location. Sentinel-5P satellite is used for collecting CO, NO₂, O₃, and SO₂ whereas the Landsat 8 satellite is used for collecting surface temperature and land cover. The data are processed on Google Earth Engine. The potential locations for urban forests in Jambi City categorized into three priority classes, namely priority 1 with the highest pollutant and surface temperatures values which are the Danau Teluk, Jambi Timur and Pelayangan District; Priority 2 with medium pollutant and surface temperatures values includes of the Danau Sipin, Jelutung, Paal Merah, and Pasar Jambi Districts; and Priority 3 with the lowest pollutant and surface temperature value.
Keyword: Air pollution, surface temperature, urban forest

Judul: Prioritas Pengembangan Hutan Kota di Jakarta Selatan
Abstrak: South Jakarta is one of the densely populated municipality in DKI Jakarta. Increased urbanization causes degraded lands green space that will lead to environmental degradation such as air pollution and rising temperatures. Urban forest is one of green space that is capable of restoring the balance and healthy urban environment. Criteria in the selection of priority areas using the variable surface temperature and air pollutants and classified by three classes of priority. Urban forest priority location is the availability of green space that goes into the priority class . The results of this study indicate priority areas in the first class of 1269.86 ha ( 8.72 % ) , the second class of 7076.55 ha ( 48.61 % ) and the third class of 6210.03 ha ( 42.66 % ) . The availability of green space that was in the first priority class of 77.96 Ha , the second class of 327.57 Ha and class of 983.61 Ha . Total green space can not be found to reach 10 % of the South Jakarta areas because land limitation and convertion land use.
Keyword: surface temperature., urban forest, temperature, priority location, green space, air pollutants

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.