Judul: Penapisan dan Identifikasi Senyawa Antijamur Patogen Phellinus lamaoensis dari Jamur Endofit pada Tanaman Obat Asal Cirebon
Abstrak: Endophyte fungal is a fungi that grows in the plant tissue which capable of producing antibiotics compounds. Intended use of these fungi to produce antifungal compounds that can inhibit the growth of fitopatogen cocoa plants Phellinus lamaoensis. The methods used in this study, among others, active screening isolates with testing activities, using the analytical KLT, analytical HPLC purification , and identification of active compounds using LC-MS. Endophytic fungi isolates used in this study comes from the leaf tissue, flowers and medicinal plants stems from Cirebon. On the leaves of plants bandotan ( ageratum conyzoides l. ) found activity endophyte fungal against pathogenic Phellinus lamaoensis in diameter clear zone with three times reprocability, successive 10.52 mm ,10.37 mm, and 9.8 mm. Fungus that has the activity called Penicillium Lagena. The presence of a compound capable of inhibiting the growth of Phellinus lamaoensis characterized by the presence of two peaks on the results of the analysis of HPLC. The first peaks had a retention time at 10.34 min on 231.8-553.2 nm as the optimal wavelength and the second peaks had a retention time of 12.152 min on 229.5-542 nm. The molecular weight of the first compound was estimated to be 206.0547 gram/mol on Liquid chromatography–mass spectrometry (LC-MS) analysis. These compound was predicted as scoparone, citropten, 4-acetoxycinnamic acid.
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Judul: Identifikasi Cendawan Endofit Asal Tanaman Obat dan Penapisan Potensinya sebagai Antibakteri
Abstrak: Endophytic fungi are microorganisms living in plant tissue without causing any harm to its host. The fungi can be found in all plants including medicinal plants. Endophytic fungi produced variety of bioactive compounds such as antibacterial substances. This research aimed to identify 3 isolates of endophytic fungi JBa8, JMa3, and SLbt3 which were isolated from medicinal plants and to screen their potency as source of antibacterial compounds. Identification of endophytic fungi was performed based on morphological characteristics and DNA barcode using ITS region. Antibacterial test was conducted against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Enteropathogenic Escherichia coli (EPEC) K1-1, and microflora E. coli by antagonism technique and agar diffusion method using mycelia extract with methanol solvent. The inhibition activity of fungal extract on bacterial tested was observed on the day 3rd, 7th, 10th, and 14th after incubation by measuring clear zone around the colonies. The 3 isolates were tentatively identified as Fusarium solani for JBa8, Fusarium sp. for JMa3, and Colletotrichum sp. for SLbt3. Antibacterial activities were observed in F. solani and Fusarium sp. which were isolated from jati belanda (Guazuma ulmifolia) and jahe merah (Zingiber officinale), respectively. F. solani inhibited the growth of EPEC K1-1, whereas Fusarium sp. suppressed P. aeruginosa with clear zone produced was 12 mm. Fusarium sp. had antibacterial activity similar with that of 8 μg/mL cefotaxime positive control. The F. solani antibacterial activity, however, was lower than the two positive control concentrations used. Colletotrichum sp. isolated from sambiloto (Andrographis paniculata) did not have any antibacterial to all bacteria tested.
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Judul: Model Proyeksi Demografi Uniradix Multistate dan Aplikasinya Dalam Status Perkawinan
Abstrak: Demografi multistate merupakan pemodelan penduduk dengan transisi aolar slale, yaitu transisi aolar status sosia! yang dialami individu-indiviclu selring dengan perubahan \Vakiu. Pergerakan transisi ini dipengaruhi oleh faklar pendidikan. ekonomi. dan pemikahan. Transisi status sosial ini dapa! terjadi dalam satu wilayah, dua wilayah stau lebih
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