Judul: Tingkah Laku Investigative dan Lokomosi serta Grooming DOmba Garut Dengan Jenis Pakan dan Waktu Pemberian Pakan Yang Berbeda
Abstrak: Tingkah laku ternak dipengaruhi oleh kondisi internal dan eksternal seperti kondisi fisiologis dan lingkungan, manajemen pemeliharaan atau pakan ternak. Tujuan dari penelitian ini adalah untuk Mengevaluasi pengaruh dari pakan limbah tauge, rumput lapang, dan waktu pemberian pakan terhadap tingkah laku grooming dan lokomosiserta investigative dari domba garut. Enam belas domba jantan dibagi menjadi dua kelompok dimana tiap kelompok diberi perlakuan pakan dan waktu pemberian pakan yang berbeda.Bentuk dari rancangan penelitian ini adalah RAK factorial pola 2x2, dimana faktor pertama adalah jenis pakan dan faktor kedua adalah waktu pemberian pakan(pagi dan malam). Hasil menunjukan bahwa tidak ada interaksi antara perbedaan ransum dan waktu pemberian pakan, akan tetapi jenis ransum mempengaruhi durasi tingkah laku grooming dan frekuensi dari tingkah laku grooming, lokomosi dan investigative.Dari penelitian ini dapat disimpulkan bahwa penambahan limbah tauge membuat domba lebih nyaman.
Keyword: Bean sprout waste, Garut sheep, Grooming, Investigative, Locomotion, Animal behavior, Physiological condition, Husbandry management, Feeding time, Factorial randomized

Judul: Respon Fisiologis dan Tingkah Laku Domba Garut dengan Pakan Limbah Tauge atau Kangkung Kering sebagai Pengganti Rumput
Abstrak: Garut sheep is commonly reared in West Java and usually fed with grass as the main feed and energy source. However, this grass has low quality and seasonally available, therefore should be an alternative feed such as mung bean sprout waste and dried kangkung. This research aimed to study sheep physiology and behavior as a response to diet and environment. This research used nine garut sheep (I0) with three treatments and replicates. The treatments were P0 (50% grass+50% consentrate), P1 (50% mung bean sprout waste+50% concentrate) and P2 (50% dried kangkung+50% concentrate). Physiological response data were analyzed by analysis of variance (ANOVA) and tested by Tukey post test method, while behavior frequency data were analyzed by Kruskal-Wallis test. Result showed that diet treatment did not affect (P>0.05) the heart and respiration rate, but significantly affected (P<0.05) on rectal temperature. All behaviors parameters were not significantly different (P>0.05) except for resting, grooming, licking strange object, and locomotion were higher in P1 than P0 and P2.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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