Judul: Klasifikasi Vegetasi Sub Zona Pegunungan, Gunung Salak, Bogor Jawa Barat
Abstrak: The research objectives were classifying sub mountain zone of Salak Mountain’s vegetation structurally, physiognomically, floristically; studying the relationship between floristic vegetation types and abiotic factors; and the alteration of structure and species composition in each vegetation type along environmental gradient. Sampling was done with systematic sampling with random start. Vegetation alliances were determined by ordination with factor analysis, and vegetation associations were determined with cluster analysis. Vegetation types at physiognomic and structural level were determined based on UNESCO and NVCS vegetation types. U-Mann Whitney statistic was employed to know whether there were abiotic factors differentiations between alliances and between vegetation structures. Chi-square test was used to know the relationship between vegetation associations and abiotic factors, also the preference of dominant tree species toward abiotic factors. There are three vegetation types founded at alliance levels, which are Schima walichii-Pandanus punctatus/Cinchona officinalis forest alliance (alliance 1); Gigantochloa apus-Mallotus blumeanus/ C. officinalis forest alliance (alliance 2); and Pinus merkusii-Athyrium dilatatum/ Dicranopteris dichotoma forest alliance (alliance 3). Alliance 1 is a broad leaf mix forest dominated by Schima wallichii, alliance 2 is a bamboo forest dominated by G. apus, and alliance 3 is a planted forest dominated by P. merkusii. The abiotic factors that consistently differentiate alliance 1 with the other alliances are N total, dust content of the soil, and slope. P soil’s content for alliance 2, while C organic soil’s content and cation exchange capacity of the soil for alliance 3. Successively, there are 5, 6, and 7 vegetation associations at alliance 1, 2, and 3. Stand structure of alliance 1 and 3 forming J reverse curve. The mean value of dominant tree species diversity and evenness index increases from alliance 2 to 3, and to 1, while the mean value of dominant tree species richness index increases from alliance 3 to 2, and to 1. Each dominant tree species has clumped distribution pattern and uniquely preference to abiotic factor.
Keyword: Cluster Analysis, Alliance, Ordination, West Java, Vegetation Association

Judul: Klasifikasi vegetasi gunung endut, Taman Nasional Gunung Halimun Salak, Banten
Abstrak: Perubahan status kawasan Gunung Endut dari hutan lindung menjadi bagian dari Taman Nasional Gunung Halimun Salak dan belum banyak diketahuinya data kuantitatif flora mengantarkan pentingnya penelitian mengenai klasifikasi vegetasi di kawasan tersebut. Klasifikasi tipe vegetasi juga merupakan salah satu faktor penting untuk kegiatan pengelolaan. Penelitian ini bertujuan untuk mengklasifikasi vegetasi Gunung Endut secara fisiognomi, struktural dan floristik, serta mengkaji kaitan antara tipe vegetasi floristik dengan faktor abiotik dan perubahan struktur dan komposisi jenis tipe vegetasi floristik di sepanjang gradien lingkungan di Gunung Endut. Sampling dilakukan secara systematic sampling with random start. Tipe vegetasi tingkat aliansi ditentukan melalui ordinasi dengan analisis faktor dan asosiasi vegetasi ditentukan dengan analisis klaster. Tipe vegetasi tingkat fisiognomi struktural ditentukan dengan mengacu pada tipe vegetasi UNESCO dan NVCS. Statistik U-MannWhitney digunakan untuk melihat perbedaan faktor abiotik antara aliansi dan perbedaan antara struktur vegetasi. Uji Chi-Square (dan Spearman) dimanfaatkan untuk melihat hubungan antara asosiasi vegetasi dengan faktor abiotik, serta melihat preferensi pohon dominan terhadap faktor abiotik.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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