Judul: Hubungan Antara Kelas Jalan dengan Kecenderungan Inkonsistensi Pemanfaatan Ruang di Kota Bogor
Abstrak: Rapid development and increasing population led to the need for space in Bogor Municipality. The increase will impact on the diversity of activities and land uses, especially for the built up area. Various land use types, land use changes and inconsistencies of land use to Regional Spatial Plan (RTRW) impact on increasing rise of traffic and street load in the Bogor Municipality. In attempt to support efforts to overcome existing problems related to those issues, spatial information system to monitor the inconsistencies are required, especially the inconsistencies in areas along the main roads in Bogor Municipality. The spatial distribution pattern of built-up areas in the Bogor Municipality tend to grow linearly follow the main road structure.
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Judul: Spatial Planning Inconsistency Analysis Viewed from Regional Physical Aspect; A Case Study Kabupaten and Kota Bogor
Abstrak: Regional spatial planning (RTRW) is a general guidance which is used for landuse establishment of a region for a certain period of time. The establishment is based on the consideration of land physical characteristics, socio-economic, politics and cultures. In fact, in the implementation, the considerations are more on socioeconomic, politics and culture than on land physical characteristics. At some stage this could result in disasters such as landslides and floods. In the years 2002 and 2003 floods occurred in Jakarta and almost 60% of the area was inundated (Direktorat Jendral Penataan Ruang 2003). This occurrences apparently resulted from reductions of water infiltration area especially in the upland of Bogor. Referring to the mentioned problems, this study aim (1) to analyze the consistency of RTRW and to determine the prediction factors influencing inconsistency of RTRW; (2) to analysize land cover changes in 1997–2002; and (3) to identify centers area of land cover changes. Analysis methods being used are: (1) Geographic Information System (GIS) for spatial analysis (maps); (2) Principal Component Analysis (PCA) to identify prediction determining factors of RTRW consistency; (3) Multiple Regression Analysis for analyzing correlation between RTRW inconsistency and determining factors of RTRW consistency; (4) Location Quotient (LQ) to identify center areas of land cover change in Kecamatan. The result shows that up to the year 2002 in the two study areas, both are showing indication of inconsistence of spatial planning, the RTRW has been inconsistence at around 22.05% in Kabupaten Bogor , and 5.76% in Kota Bogor. In term of, land cover changes showing in the time range of five years in the built -up areas an increased up to 30.46% for Kabupaten Bogor and 3.45% for Kota Bogor.
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Judul: Primer Spesifik Ikan Hiu Biru (Prionace glauca) Berbasis Marka Cytochrome B untuk Autentikasi Produk Olahan Ikan Hiu
Abstrak: Primer merupakan aspek krusial yang perlu diperhatikan dalam proses mengidentifikasi spesies pada produk olahan hiu berbasis genetik. Jenis marka mtDNA yang dapat digunakan untuk mengidentifikasi species salah satunya adalah gen cytochrome b. Prionace glauca merupakan spesies ikan hiu yang tersebar luas di perairan Indonesia yang jumlah populasinya terus mengalami penurunan. Mendesain primer cytochrome b spesifik Prionace glauca menjadi tujuan dalam penelitian untuk mengidentifikasi adanya spesies tersebut dalam produk olahan ikan hiu komersil. Primer didesain pada fitur Primer-BLAST pada situs NCBI. Primer diujikan melalui proses amplifikasi pada isolat DNA produk olahan hiu yang meliputi ikan hiu asin (A1), abon ikan hiu (B1 dan B2), ikan hiu asap (C1), ikan hiu empal (E1), dan fillet ikan hiu (F1 dan F2) dengan tingkat kemurnian 0.909-2.106 (A260/280). Pada suhu annealing 55oC teridentifikasi ukuran DNA seluruh sampel yang diujia dalah 500 bp. Sekuens dari sampel yang telah diamplifikasi menunjukkan hasil BLAST spesies yaitu Prionace glauca dengan tingkat kemiripan mencapai 99,66%. Kedekatan genetik pada hasil filogenetik terlihat pada kelompok yang terbentuk dengan spesies target Prionace glauca dengan nilai bootstrap 100%., Primer is an essential aspect of the species identification procedure. One of the DNA sourced at cytochrome B mtDNA is recognized suitable as a marker. Shark species that distribute almost in every Indonesia's sea is Prionace glauca. A pair of specific cytochrome b primers were designed with the Primer-BLAST feature on NCBI. The primer got applied at DNA amplification that run to analyze some products labeled as salted shark meat (A1), shark meat floss (B1 and B2), smoked shark meat (C1), shark empal (E1), and shark meat fillet (F1 and F2), have 0,909-2,106 (A260/280) purity rate. The sizes of all DNA samples were identified at 500 bp at 55oC annealing temperature. Prionace glauca was spotted at BLAST result from the sample's sequence with the percent identity up to 99,66%. The phylogenic tree shows a group containing product and Prionace glauca with 100% bootstrap value.
Keyword: Prionace glauca, Cytochrome b, Ikan hiu, Primer spesifik