Judul: Analisis respon konsumen terhadap performance Box Office Coffee Shop, Bogor
Abstrak: Kota Bogor sebagai daerah pariwisata merupakan kota yang terkenal dengan udaranya yang sejuk dan nyaman sehingga dikunjungi oleh masyarakat Jakarta dan sekitarnya untuk bersantai dan berlibur. Hal ini disebabkan karena Bogor masih menjadi salah satu kota pilihan untuk dikunjungi di akhir minggu. Dengan tingginya mobilitas kota Bogro tersebut maka semakin mendorong berkembangnya usaha-usaha makanan dan minuman siap santap dalam bentuk restoran atau kafe di Kota Bogor. Kondisi tersebut menyebutkan meningkatnya permintaan terhadap usaha yang menyediakan makanan dan minuman siap santap.
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Judul: Analisis keputusan pembelian dan kepuasan konsumen coffee shop de koffie pot, Bogor
Abstrak: Gaya hidup yang berubah pada masyarakat Indonesia belakangan ini menyebabkan manusia semakin menginginkan nilai lebih dari sekedar makan sebagai pemuas kebutuhan fisiologis. Pola konsumsi pangan masyarakat kota Bogor yang juga telah berorientasi pada kepraktisan dalam mengkonsumsi dan kesenangan dapat dipenuhi oleh adanya restoran dan kafe yang jumlahnya terus berkembang di kota Bogor. Adanya pertumbuhan jumlah restoran dan kafe serta dengan hadirnya coffee shop di kota Bogor baru-baru ini telah menyebabkan beragamnya alternatif pilihan bagi konsumen.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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