Judul: Analisis Finansial Budidaya Sengon dan Salak Pada Sistem Pengelolaan Agroforestri di Desa Kalimendong Kecamatan Leksono, Kabupaten Wonosobo
Abstrak: Agroforestry is a community forest management system that is able to maximize the utilization of land. Agroforestry has been widely applied in Wonosobo especially Kalimendong Village, with primary plant is sengon and secondary plant is salak. The purpose of this research are determine the financial feasibility of sengon and salak agroforestry system, and measure the perception level of farmer regarding to the benefits of cultivation between sengon and salak. Community forest management with agroforestry systems in KalimendongVillage is worth to develope because its NPV that reached of Rp 233.261.645, BCR of 4,61 and an IRR of 74,83%. Monoculture of salak plantation system considered to be more profitable than other types of management, but the agroforestry management system application still expected to be the main priority of its benefits economy, social and ecology.
Keyword: perception., financial analysis, Management of agroforestry

Judul: Sistem Pengelolaan dan Kinerja Finansial Berbagai Model Agroforestry di Kabupaten Wonosobo, Jawa Tengah
Abstrak: Permintaan kayu yang tinggi menumbuhkan inisiatif masyarakat untuk membangun hutan rakyat, namun masih banyak masyarakat yang belum mengetahui sistem pengelolaan hutan rakyat yang baik dan dapat menguntungkan secara finansial. Wonosobo bagian utara dalam pengembangan hutan rakyat, masih banyak masyarakat yang menanam tanaman kehutanan hanya sebagai batas kepemilikan lahan saja karena pendapatan dari tanaman kehutanan tersebut dianggap kurang menguntungkan. Tujuan dari penelitian ini adalah untuk mengidentifikasi sistem pengelolaan agroforestry dan alasan pengembangan di lokasi penelitian, mengetahui kelayakan usaha secara finansial pada pengelolaan hutan rakyat, mengetahui sistem pemasaran kayu, serta mengetahui kontribusi pendapatan petani terhadap pendapatan total di Kabupaten Wonosobo. Penentuan responden dengan menggunakan metode stratified random sampling dengan jumlah responden sebanyak 45 orang yang didapat dari rumus slovin. Penelitian ini menggunakan pendekatan kualitatif yang dilakukan dengan metode survey menggunakan alat bantu kuesioner dan didukung oleh teknik kuantitatif. Terdapat dua tipe agroforestry yang terdapat di Wonosobo, agrisilvicultural (albasia-cabe untuk tipe I, albasia-kopi untuk tipe III dan albasia-salak untuk tipe IV) dan silvopastural (albasia-HMT untuk tipe II). Hasil penelitian menunjukkan nilai kelayakan usaha hutan rakyat di Kabupaten Wonosobo pada berbagai strata kepemilikan lahan dari tipe agroforestry berdasarkan analisis finansial pada tingkat suku bunga 6,5% (suku bunga yg berlaku di Bank Indonesia tahun 2010) layak untuk diusahakan. Berdasarkan analisis pertambahan nilai (incremental analysis) dengan menghitung Δ BCR tiap strata, model agroforestry yang paling layak untuk diusahakan adalah pada tipe IV. Berdasarkan analisis sensitivitas, tipe IV masih layak diusahakan pada saat adanya peningkatan biaya operasional dan saat adanya penurunan produksi total sebesar 10%, 20% dan 30%. Hal tersebut membuktikan bahwa tidak ada pengaruh yang besar (tidak sensitif) terhadap perubahan NPV pada tipe IV sampai dengan perubahan 30%.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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