Judul: Desain Model Kurikulum Berbasis Go Green School
Abstrak: Population is increasing, on the other hand the availability of natural resources remains the same, hence, it is necessary to use advanced technology to increase productivity and to use natural resources properly for the prosperity of human beings. But this could also give negative impacts to the environment. Egoism and moral crisis are considered as factors which cause the environment destruction. This situation needs to be overcome through strategic way to young generation on character building which is integrated in the curriculum. The Go Green School Model (GGS) curriculum design of Tarakanita is based on the Education Unit Level Curriculum (EULC). System approach is used for designing the ideal mode is based on the 8 criteria of National Education Standards as stipulated in the Government Bylaws No. 19 in 2005. The performance of the model is then compared to currently-used curriculum and validated to the pre-determined sample of fourth-grade elementary students for one semester. The result shows that the understanding and creativity ( factors of environmental content) of the students treated using the curriculum of GGS tend to be higher than that of the students treated using the Education Unit Level Curriculum . To shift from the Education Unit Level Curriculum Curriculum to the GGS curriculum needs a linking step that is the Teaching Unit Curriculum Plus (EULC +).
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Judul: Desain ekopesantren dalam kerangka pembangunan berkelanjutan
Abstrak: Developing environmental awareness among Muslims in Indonesia is an important effort and should continue to get attention. There was no resistance in the Islamic scholars in Indonesia when talking about environmental issues, because they had seen very severe environmental damage and disasters in Indonesia. This dissertation tries to formulate a design for the environmentally friendly Islamic Boarding School Program (Ecopesantren) on the framework of sustainable development. The research conducted in purposive sampling to the three Pesantren in West Java and Banten Province, was done at the leading pesantren (Pesantren Induk) in both provinces: Daar El Qolaam, Banten, Al Musaddadiyah, Garut and Pesantren Buntet, Cirebon. The research was conducted using complex pairs of research series such as Participatory Action Research (PAR), Institutional Development Framework (IDF), Non-Parametric Spearman Correlation, Analytical Hierarchical Process (AHP), Interpretative Structural Modelling (ISM) and Focus Group Discussion (FGD). In general, the findings show that according to the IDF index (I) for institutional and resource management, human resources, external resources, and financial resources, all the three institutions scale ranges at the IDF(I) 3- 4, which shows that the institution was mature and sustainable. But for the activities related to environmental programs the index IDF (I) points on a scale of 1-2 which refers to the early stages. On the other hand the status of environmental awareness in all the students indicated that they have moderate level of sustainable development awareness (mean=3.86; sd=0.86). They also believe Islam teaches about environment -- Islamic environmentalism – guiding to good practice towards the environment (90.38%). The expert survey suggests that the purpose of the ecopesantren program should mainstream the social and cultural aspect (AHP=0.443), it also recommends that the government (Ministry of Environment – MoE) has an important role (AHP=0.301) in supporting the ecopesantren program. The MoE can initiate the formation of a "National Ecopesantren Team (TNE)" in cooperation with local governments and institutions , in order to initiate a national program of ecopesantren. On the other hand, it is necessary for the pesantren internally to form an “environmental task force” that is responsible for creating programs tailored to local environment-related issues and priorities. Besides the capacitybuilding necessary to transform the ecopesantren program, it is necessary to perform monitoring and evaluation of a tailored action plan that can achieve the targets set.
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Judul: Stabilitas Protein Rekombinan Interferon Alfa-2b Manusia Pada Pichia Pastoris.
Abstrak: Protein rekombinan interferon alfa-2b manusia (rhIFNα-2b) merupakan protein terapeutik yang diaplikasikan untuk penanganan hepatitis dan kanker. Pengembangan bentuk sediaan rhIFNα-2b yang stabil tetap menjadi tantangan. Penelitian ini bertujuan untuk mempelajari stabilitas rhIFNα-2b pada tiga parameter, yaitu suhu, pH dan kondisi lama penyimpanan. Overproduksi rhIFNα-2b dilakukan menggunakan sistem ekspresi Pichia pastoris (P. pastoris) yang ditumbuhkan pada media Buffered Methanol Complex (BMMY) pada suhu 30 °C selama 48 jam dan 2% metanol sebagai induser. MinimateTM tangensial flow filtration system dengan molecular weight cut off (MWCO) 5 kDa digunakan pada proses filtrasi protein. Protein yang telah difiltrasi kemudian dipurifikasi dengan AKTA purifier 10 system menggunakan kolom His-trap. Kuantifikasi protein menggunakan bicinchoninic acid assay menunjukkan bahwa yield rhIFNα-2b hasil purifikasi sebesar 10.92 mg/L (OD600 = 2.3). Karakterisasi bobot molekul menggunakan analisis Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) dan Western Blot menunjukkan bahwa protein tersebut merupakan rhIFNα-2b dengan bobot molekul sekitar 24 kDa. Karakterisasi identitas protein berbasis spektrometri massa Matrix Assisted Laser Desorption Ionization-Time Of Flight/ Time Of Flight (MALDI-TOF/ TOF) menginformasikan bahwa protein tersebut teridentifikasi sebagai hIFNα-2b dengan urutan asam amino yang mewakili 22% dari sekuen secara keseluruhan. Analisis SDS-PAGE non reducing dan software Image J menunjukkan bahwa peningkatan suhu, kondisi pH asam dan basa serta kondisi lama penyimpanan telah memacu terjadinya aggregasi dan degradasi rhIFNα-2b. Uji 3-[4.5-dimethyltiazol-2il]-2.5-diphenylltetrazolium bromide (MTT) menunjukkan bahwa aggregasi dan degradasi menurunkan aktivitas antiproliferasi rhIFNα-2b terhadap sel MCF-7. Kondisi kenaikan suhu, pH asam dan basa serta lama penyimpanan dapat mempengaruhi stabilitas rhIFNα-2b. Kondisi stabil rhIFNα-2b dicapai pada suhu 4 °C, pH 7 selama 2 hari waktu inkubasi. Data tersebut dapat digunakan untuk mengembangkan formulasi rhIFNα-2b sebagai protein terapeutik.
Keyword: rhIFNα-2b, Pichia pastoris, stabilitas, aggregasi, degradasi