Judul: Isolasi Senyawa Lutein dari Ekstrak Bunga Brokoli sebagai Antioksidan
Abstrak: Antioxidant activity is important to protect our body from free radicals. Green vegetables such as broccoli has high content of lutein which can act as an antioxidant. The objective of this study was to isolate and characterize lutein extract of broccoli flowers, and then further evaluated its antioxidant activity by using α,α-diphenyl-β-picrylhydrazyl (DPPH). The extraction used n-hexane as solvent and KOH/ethanol for saponification process. Extracted lutein was characterized with preparative thin layer chromatography (TLC). Four fractions were obtained from preparative TLC and fraction 3 had the same Rf compared with lutein standard. This fraction was further analyzed with Fourier transform infrared (FTIR) spectrophotometer and had similar functional groups with lutein standard. Based on this result, it can be concluded that broccoli flower extract has lutein compound and potential as an antioxidant with 99.321 μg/mL IC50 value.
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Judul: Analysis of Active Compounds in Broccoli (Brassica oleracea L var. italica) to Inhibit Kelch-like ECH-associated Protein 1 in Type 2 Diabetes Mellitus Patients
Abstrak: Diabetes Melitus tipe 2 ditandai dengan resistensi insulin akibat kerusakan reseptor insulin, terjadi karena peningkatan jumlah reactive oxygen species (ROS). Hal ini dapat diatasi dengan mengaktifkan antioxidant response elements (ARE) dengan menginhibisi regulator Kelch-like ECH-associated protein 1 (KEAP1). Dimetil fumarat (DMF) telah dipakai sebagai obat penurun jumlah ROS namun dengan efeksamping yang berbahaya, dilakukan pencarian senyawa obat lain. Senyawa lain yang telah terbukti menurunkan ROS ialah senyawa yang berasal dari brokoli (Brassica oleracea. var. italica), yaitu sulforafan. Brokoli memiliki 31 senyawa aktif yang diuji aktivitas dan potensinya menggunakan metode in silico dengan penapisan virtual dan penambatan molekuler. Tahap penapisan virtual mengeliminasi 5 senyawa dan uji toksisitas mengeliminasi 8 senyawa, sehingga tersisa 18 senyawa yang lolos ke penambatan molekuler. Analisis penambatan menggunakan parameter energi afinitas dan persentase Binding Site Similarity, menunjukkan terdapat tiga kelompok senyawa yang berpotensi dengan gluconasturtiin sebagai senyawa dengan potensi terbaik. Senyawa-senyawa tersebut harus diuji lebih lanjut terutama terkait efek sampingnya sebelum dijadikan sebagai senyawa obat., Type 2 Diabetes Mellitus can be characterized by insulin resistance, as a result from insulin receptor damage from increased amount of reactive oxygen species (ROS). This problem is overcome by inhibiting Kelch-like ECH-associated protein 1 (KEAP1) regulator and activating antioxidant response elements (ARE). Dimethyl fumarate (DMF) is one of the drug that can reduce the amount of ROS, but because of its dangerous side effects thus performed search for other drug-like compounds. Compound proven to reduce ROS is sulforafan that derived from broccoli (Brassica oleracea var. italica). There are 31 active compounds in broccoli, tested its activity and potency by in silico method, that is by virtual screening and molecular docking. Virtual screening eliminated 5 compounds and toxicity test eliminated 8 compounds, allowing only 18 compounds to molecular docking step. Analyzed by their affinity energy and Binding Site Similarity percentage, there are three groups of potential compounds, with gluconasturtiin that has the best potential. These compounds should be carried out for further analysis, especially their side effects, before being categorized as a drug.
Keyword: brokoli, diabetes melitus, in silico, KEAP1, ROS

Judul: Desain dan konstruksi kapal payang di Pamekasan Madura
Abstrak: Pembangunan kapal ikan di Indonesia pada umnumnya dibangun di galangan kapal tradisional. Pembangunananya tidak dilengkapi dengan gambar-gambar desain, seperti general arrangment, lines plan, dan deck profile construction. Kapal ikan yang dominan di Pamekasan adalah kapal payang. Tujuan penelitian untuk mendokumentasikan teknik dan tahapan pembangunan kapal payang di Pamekasan; mendokumentasikan desain dan konstruksi kapal payang Pamekasan; dan menghitung nilai parameter hidrostatik serta stabilitas kapal payang di Pamekasan Madura.
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