Judul: IPB (Bogor Agricultural University)
Abstrak: KHV has made mass mortality in koi and common carp, and spread out to many countries. In Indonesia, KHV has caused outbreaks of mass mortality in many provinces, and cause economic losses and significant social matter. The aim of this research are to recognize and to analyze genetic variation of KHV that infects Cyprinus-carpio and to recognize its biogeographic distribution in Indonesia as well as to recognize and to analyze tissues pathological changes which infected by KHV. In this research, the fish that have been used are koi and common carp which suspected infected by KHV according to the clinical symptoms derived from 20 provinces in Indonesia. In every fish sample was taken its gill to PCR examination and then was done sequencing DNA (for KHV positive samples), for histopathology and immunohistochemistry examination, the organs that were taken: gill, kidney, spleen, intestine or digestive tract, liver, heart, and brain. Based on the results of sequencing DNA KHV and phylogenetic tree construction that has been made, there are 17 variants from 18 samples KHV positive was found. Those variants can be grouped into two clusters the main branch which consisted of group 1 includes variants KHV from South Kalimantan, Lampung, West Papua, West Kalimantan, West Java, Bali, East Nusa Tenggara. Then group II consists of KHV variants from North Sumatra, West Kalimantan, West Nusa Tenggara, Riau, East Kalimantan, and DKI Jakarta. Related to the infections of KHV variants on koi and common carp, pathological changes were found in such organs (gill, spleen, kidney, intestine and digestive tract, liver, heart, and brain). Pathological changes in gill organ was found proliferation of epithelial cells and fusion of secondary lamella, hypertrophy epithelial cells of gill lamella, telangiectasis, the inclusion body, edema, proliferation of hyaline layer and fibrosis at the base of the gills. Spleen pathological change was found infiltration of inflammatory cells or lymphocytes, appeared MMC, hemorrhage, congestion, and edema. Kidney pathological change was found hemorrhage, proliferation of cells in the interstitial, MMC, thickening of the tubule, inflammation of the glomerulus, the inclusion body, congestion, edema, fibrosis, necrosis of the glomerular. Intestine and digestive tract was found hemorrhage, enteritis, proliferation, goblet cells, fusion of the villi, deposit enterolit on gastric, congestion, edema, and necrosis in krypta. Liver pathological change was found hydropic degeneration, perivascular cuffing, congestion, and fibrosis. Heart pathological change was found infiltration of inflammatory cells or lymphocytes in the endocardium, hemorrhage, epicarditis, pericarditis, myocarditis, vacuolization, congestion, edema, fibrosis, and necrosis.
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Judul: Karakterisasi Koi Herpesvirus (KHV) pada Ikan Mas Cyprinus carpio di Danau Toba.
Abstrak: Koi herpesvirus (KHV) merupakan salah satu faktor pembatas dalam kegiatan budidaya ikan mas dan koi (Cyprinus carpio). Penelitian ini bertujuan untuk mengidentifikasi karakteristik koi herpesvirus (KHV) pada ikan mas yang terdapat di Danau Toba melalui analisis virus dengan uji polimerase chain reaction (PCR), sekuensing DNA, pengamatan gejala klinis dan makroanatomi, serta konfirmasi histopatologi jaringan ikan. Hasil yang didapatkan ialah gen thymidine kinase (TK) KHV yang didapatkan memiliki panjang pita DNA sebesar 411 bp. Gen TK KHV pada sampel memiliki kemiripan susunan basa nitrogen sebesar 98% dengan strain yang sebelumnya teridentifikasi berdasarkan hasil blast di data GeneBank, yakni dengan strain KHV Iran (KX609547.1), strain KHV A2 Indo_7C04NS1 (HM347106.1), strain KHV-Eropa (AB375385), strain KHVJepang (AP008984), dan strain KHV-USA (DQ657948). Gen TK KHV yang diidentifikasi telah mengalami mutasi berupa transisi dan transversi pada susunan basa nitrogennya. Infeksi KHV pada ikan mas yang didapatkan menunjukkan terjadinya gejala klinis, berupa kelainan tingkah laku, kelainan eksternal tubuh, dan kelainan organ internal tubuh ikan. Berdasarkan hasil histopatologi juga didapatkan terjadinya fusi dan pembentukan badan inklusi pada lamella dan gigi-gigi insang sebagai bukti infeksi virus KHV pada ikan.
Keyword: Blast, ikan mas, KHV, mutasi, PCR, sekuensing DNA

Judul: Sintesis dan Pencirian Natrium Titanat (Na2Ti2O4(OH)2) Berbahan Dasar Titanium Dioksida
Abstrak: TiO2 can be utilized as basic material for synthesis of sodium titanate (Na2Ti2O4(OH)2). This research was purposed to synthesize and characterize sodium titanate from TiO2 rutile powder as a basic material. Sodium titanate was synthesized by hydrothermal process at temperature 130 °C by mixing TiO2 rutile powder with 5 M and 10 M NaOH solution, then each was calcinated at temperature 700 °C. The energy dispersive X-ray analysis showed that sodium titanate has been successfully synthesized. This result was also supported by functional group analysis with Fourier transform infrared (FTIR) spectrofotometer showing the existence of Ti-O-Na vibration band at 989.42 cm-1. The formation of sodium titanate was depend on the reaction condition such as temperature, reaction time, and NaOH concentration. Scanning electron microscope image indicated that sodium titanate synthesized at 130 °C in 20 hours reaction time with 10 M NaOH had crystal size of 99.37 nm.
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