Judul: Komposisi Jenis dan Struktur Vegetasi pada Kawasan Karst Gunung Cibodas, Kecamatan Ciampea, Kabupaten Bogor.
Abstrak: Karst area is carbonated rock area that showing karst morphology, with high valued ecosystem of flora and fauna. One of the karst areas in West Java that has quite alarming condition is Gunung Cibodas, Ciampea subdistrict, Bogor regency. The purpose of the research is to analyze the composition and structure of vegetation in the karst area of Gunung Cibodas. The location of observation determined by systematic sampling with random start metode. Analysis vegetation plots are defined based on the direction of the slope and the mouth of the cave. The research found 30 species of seedlings, 32 species of saplings, 2 species of poles, 6 species of trees and 92 species of understorey vegetations. On the direction of the slope and the mouth of the cave is dominated by pioneer species such as Leucaena leucocephala, Calliandra calothyrsus and Macaranga peltata which show the process of secondary succession, moreover the understorey is dominated by the species of Invasive Alien Species (IAS). Stand structure inverted J-shaped curve can only be found on the northern slopes and generally canopy stratification in this karst area consists of three strata, namely stratum C, D and E with the dispersion pattern of this species of dominant groups in the direction of the slope and the mouth of the cave.
Keyword: stand structure, karst vegetation, composition of species

Judul: Ekologi Vegetasi dan Etnobotani Kawasan Karst Gunung Cibodas, Ciampea, Bogor
Abstrak: Karst mengandung makna sebagai suatu bentang alam yang secara khusus berkembang pada batuan karbonat (batugamping), baik berkelompok maupun tunggal dibentuk dan dipengaruhi oleh proses pelarutan (karsitifikasi) yang derajatnya lebih tinggi dibanding kawasan batuan lainnya. Vegetasi yang hidup di kawasan karst sangat khas, baik dilihat dari segi bentuk dan komposisi jenisnya. Pepohonan yang hidup di kawasan karst biasanya kecil dan bertajuk jarang, perakaran dalam dan berkelok-kelok, serta menempel pada tebing-tebing. Hubungan timbal balik antara manusia dan lingkunganny adisebut etnobotani. Nilai keanekaragaman hayati bagi masyarakat dihitung berdasarkan nilai Index Cultural of Significance (ICS). Jenis yang dominan pada tingkat pohon adalah jenis Macaranga sp. (ki bolong), pada tingkat tiang adalah Prunus avium (ceri) dan Macaranga sp. (ki bolong). Pada tingkat pancang yang dominan adalah Piper aduncum (seuseurehan) dan Calliandra calothyrsus (kaliandra). Pada tingkat tumbuhan bawah yang dominan adalah Axonopus compresus (rumput teki) dan Imperata cylindrica (ilalang). Semakin tinggi nilai ICS menunjukkan semakin tinggi nilai pemanfaatannya dalam kehidupan masyarakat. Tumbuhan yang memiliki nilai ICS tertinggi yaitu kelapa dengan nilai sebesar 157.
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Judul: Pemurnian Rekombinan Eritropoietin Manusia (rhEPO) Menggunakan Kromatografi Afinitas Ni-NTA dan Co-Talon
Abstrak: Suitable and efficient techniques for purification of recombinant human erythropoietin (rhEPO) are required to obtain pure rhEPO which can be continued to preclinical trials step. One of these techniques is immobilized metal affinity chromatography (IMAC), which has advantages such as high selectivity, easy to elute, and simple procedure for column regeneration. At least, there are 2 kinds of commercial IMAC resin using nitriloacetic acid (NTA) matrix as chelating agent, namely nickel-based (Ni-NTA) and cobalt-based IMAC resin (Co-NTA), the latter is well known as Talon. This research studied the influence of metal ions Ni2+ and Co2+ in rhEPO purification. The results indicated that Ni- NTA had higher affinity in binding rhEPO protein compared with Co-Talon. Average total protein by Ni-NTA was 226.72 μg/mL and Co-Talon was 60.52 μg/mL, but the protein purity with Co-Talon was better than Ni-NTA. Base on protein yield that can be separated, Ni-NTA is more suitable to obtain high yield of rhEPO protein.
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