Judul: Biosensor antioksidan berbasis superoksida dismutase deinoccus radiodurans diimobilisasi pada nanokomposit zeolit alam indonesia
Abstrak: The immobilization superoxide dismutase extract from D. radioduras on a zeolite nanocomposite modified electrode were studied. Cyclic voltammetry (CV) are employed to investigate the catalytic behavior of the biosensor. The superoxide radical was produced in solution using the xantine/xanthine oksidase system. Antioxidant activity vitamin C was determined by 2, 2-diphenil-2-picrylhydrazyl (DPPH) free radical scavenging method and comparing with biosensor SOD. Optimum conditions for SOD activity was at pH 9, temperature 300 C, zeolite 137.5mg, and SOD 3 U/ml for pure SOD and 9, temperature 300 C, zeolite 137.5mg, and SOD 1500μg/mL for D. radiodurans SOD. Dismutation reaction kinetics of superoxide catalyzed by SOD followed the Lineweaver-Burk kinetics with D. radiodurans SOD Kmapp value was higher than pure SOD. Antioxidant capacity for Vitamin C was 19.49 ppm using DPPH method and value 0,7 for relative antioxidant capacity using biosensor SOD. In conclusion, D radiodurans SOD immobilized on a zeolite nanocomposite had a great potential as biological recognition component for antioxidant biosensor.
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Judul: Aktivitas dan Stabilitas Superoksida Dismutase dari Ekstrak Deinococcus radiodurans yang Diimobilisasi pada Zeolit Alam sebagai Biosensor Antioksidan
Abstrak: Immobilization of superoxide dismutase (SOD) from extract of Deinococcus radiodurans have been studied. D. radiodurans is a bacteria having an enzyme Mn-SOD. The optimum condition of SOD antioxidant activity of the extract was at 31 °C, pH 9, and 157 mg zeolite. Determination of Michaelis-Menten constant was based on Lineweaver- Burk, Eadie-Hoofstee, and Hanes methods. The KMapp of SOD of the extract immobilized on zeolite was lower than that of without immobillization. Electrode stability was relatively stable up to 22 hours. Antioxidant activity of using zeolite as the matrix was higher than that of the unimmobilization. The antioxidant activity on electrode with zeolite at 22nd hour was 64.0 %, and the stability of electrodes without zeolite at the same time was only 49.3 %. The SOD of D. radiodurans is potential as a biosensor for antioxidants because of the high antioxidant activity despite of the low stability.
Keyword: superoxide dismutase, natural zeolites, Deinococcus radiodurans, antioxidant biosensor

Judul: Penggunaan Trie dalam Membangun Frequent ltemset Menggunakan Algoritmc FPGrowth
Abstrak: FP-growth algorithm search for frequent itemset using the FP-tree data structure which is a prefix tree structure based on pattern growth algorithm. Prefix tree in FP-Growth uses the trie structure in the relationship pattern among the items in the database. Trie is a tree structure that uses strings as key, where each descendant represents a character or prefix. To find frequent itemset, FP-growth algorithm build prefix tree recursively. However, recursive function may need longer time than the Iterative (Manolopoulos 2003). Goal of this research is to create alternatives iteratively to the development of frequent iternset using trie on the FP-Growth. Research was conducted on several stages for the development of the frequent itcmset using a trie, stage through pruning and without pruning. In the last stages of testing is done using the input parameters, enter the difference in support, the number of transactions, and the items involved. Testing stage compare the execution time in the computing process through the stage with pruning and without pruning. Thing that affects the execution time is the number of items involved in development process of the frequent itemset. Frequent itemset development with the pruning process will he faster than without pruning, hecause at the time to build a combination of frequent itemset, infrequent items are not in the development process.
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