Judul: Pengaruh Dukungan Sosial dan Strategi Koping terhadap Ketahanan Keluarga pada Pernikahan Jarak Jauh
Abstrak: Pengaruh Dukungan Sosial dan Strategi Koping terhadap Ketahanan Keluarga pada Pernikahan Jarak Jauh. Dibimbing oleh TIN HERAWATI dan DEFINA. Pasangan suami istri umumnya tinggal dalam satu rumah. Namun, ada kalanya keluarga tidak tinggal bersama yang disebabkan pasangan tersebut merantau untuk bekerja. Kondisi ini menyebabkan suami istri mengalami kesulitan dalam menjaga ketahanan keluarga. Tujuan penelitian adalah menganalisis pengaruh dukungan sosial dan strategi koping terhadap ketahanan keluarga pada pernikahan jarak jauh. Responden dalam penelitian ini adalah 60 istri yang menjalani pernikahan jarak jauh di eks-Karesidenan Pekalongan. Metode penelitian ini adalah non-probability sampling dengan teknik voluntary sampling. Data dikumpulkan melalui google form dengan kuesioner online. Hasil penelitian menunjukkan bahwa dukungan sosial berada pada kategori rendah, sedangkan strategi koping dan ketahanan keluarga pada kategori sedang. Strategi koping yang sering dilakukan kelurga adalah strategi koping yang berfokus pada emosi. Terdapat hubungan positif signifikan antara strategi koping dengan ketahanan keluarga. Faktor yang berpengaruh positif signifikan terhadap ketahanan keluarga yaitu lama pendidikan suami dan strategi koping., The Influence of Social Support and Coping Strategies on Family Strenght in Long Distance Marriages. Supervised by TIN HERAWATI and DEFINA. Married couples generally live in one house. However, there are times when the family does not live together because the couple migrates to work. This condition causes husband and wife to experience difficulty in maintaining family strenght. The research objective was to analyze the influence of social support and coping strategies on family strenght in long-distance marriages. Respondents in this study were 60 wives who had long-distance marriages in the ex-Residency of Pekalongan. This research method is non-probability sampling with voluntary sampling technique. Data was collected through google form with online questionnaire. The results showed that social support was in the low category, while coping strategies and family strenght were in the medium category. The coping strategy that is often used by the family is a coping strategy that focuses on emotions. There is a significant positive relationship between coping strategies and family strenght. Factors that have a significant positive effect on family strenght are the husband's length of education and coping strategies.
Keyword: dukungan sosial, ketahanan keluarga, pernikahan jarak jauh, strategi koping, coping strategies, family strenght, long distance marriage, social support

Judul: Social Support, Husband-Wife Interaction, and Marital Quality on Long-Distance Marriage
Abstrak: Pernikahan jarak jauh merupakan fenomena menarik bagi para pemerhati kehidupan keluarga, karena jarak fisik yang berjauhan diduga berpengaruh terhadap keharmonisan keluarga. Ketika salah satu pasangan tidak bisa mendampingi keluarga dalam menghadapi problematika sehari-hari, maka untuk mempertahankan kualitas perkawinan, dibutuhkan dukungan sosial dari lingkungan keluarga dan kerabat dan interaksi suami-istri yang baik antarpasangan. Penelitian ini bertujuan untuk menganalisis pengaruh dukungan sosial dan interaksi suami-istri terhadap kualitas perkawinan pada pernikahan jarak jauh. Responden dalam penelitian ini adalah 146 orang suami dan istri yang bekerja di Lembaga negara X, berasal dari keluarga utuh, sudah memiliki anak, dan sedang menjalani pernikahan jarak jauh selama minimal 6 bulan. Responden dipilih secara purposive dan pengambilan data menggunakan google form. Hasil penelitian menunjukkan dukungan sosial dan interaksi suami-istri yang dirasakan responden tergolong cukup baik. Setengah dari responden laki-laki memiliki kualitas perkawinan yang sangat baik, sementara lebih dari setengah responden perempuan memiliki kualitas perkawinan tergolong cukup baik. Hasil uji beda menunjukkan terdapat perbedaan signifikan pada interaksi suami-istri dan kualitas perkawinan dengan rataan laki-laki lebih tinggi daripada perempuan. Hasil uji korelasi menunjukkan semakin tinggi pendapatan responden, jumlah anak, dan dukungan sosial berhubungan dengan semakin tingginya kualitas perkawinan yang dirasakan. Adapun hasil uji regresi menunjukkan pada responden laki-laki, semakin kecil pendapatan, semakin banyak jumlah anak, semakin besar dukungan sosial yang dirasakan, dan semakin baik interaksi suami-istri akan semakin meningkatkan kualitas perkawinan., Long-distance marriage is an interesting phenomenon for family observers because physical distance could affect the family harmony. When one partner cannot accompany the family in dealing with daily problems, social support from the family and relatives and good husband-wife interaction between partners is needed to maintain the quality of the marriage. This study aims to analyze the effect of social support and husband-wife interaction on marital quality in long-distance marriages. Respondents in this study were 146 husbands and wives who worked at State Institution X, came from intact families, had children, and were currently in a long-distance marriage for at least 6 months. Respondents were selected purposively and data collection used the Google forms. Results showed that social support and husband-wife interaction felt by respondents were moderate. Half of male respondents had high marriage quality, while more than half of female respondents had moderate marriage quality. Comparative tests showed significant differences in interaction between husband and wife and the quality of marriage, with the average of male higher than female. Correlation test showed that higher income, number of children, and social support is associated with higher perceived marital quality. Regression test showed that for male respondents, lower income, more children, greater perceived social support, and better interaction between husband and wife will further improve marital quality.
Keyword: husband-wife interaction, long-distance marriage, marital quality, social support

Judul: Characterization and Expression of Tomato infectious chlorosis virus Coat Protein Gene in Escherichia coli
Abstrak: Tomato infectious chlorosis virus (TICV) is a member of Crinivirus genus. TICV infects tomato crop in Garut and Cipanas, West Java Indonesia. Tomato initially showed bright interveinal yellowing symptoms. The coat protein gene (CP-TICV) was amplified by RT-PCR from total RNA extracted from infected tomato leaves and the amplified fragment was cloned and completely sequenced. The fragment was subsequently subcloned into the pET-21b expression vector. The recombinant plasmid was transformed to Escherichia coli strain BL21(DE3)pLysS to express the coat protein. The coat protein fused to a 6xhistag, was purified by affinity chromatography using a NiNTA spin column. The identity of the purified protein was confirmed by SDS-PAGE. In this experiment, 792 bp of CP-TICV gene of TICV virus has been successfully cloned, sequenced, and expressed in E. coli. Based on nucleotide sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain strain and 99% identity to North America, France, and California strains and based on amino acid sequences alignment analysis, TICV-Indonesia strain showed 100% identity to TICV –Spain, North America, France strains and 99.2% identity to California strain. CP-TICV showed over expressed in E. coli when it is induced with 1 mM IPTG and incubated at 37oC. Purified CP-TICV-Histag recombinants protein sized 29 kDa based on SDSPAGE analysis.
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