Judul: Pengaruh Teknik Sterilisasi terhadap Keberhasilan Inisiasi Eksplan Paulownia (Paulownia elongata SY. Hu) secara In Vitro
Abstrak: In vitro culture is used as one of the solutions in paulownia propagation. Contamination is a major problem in procedures micropropagation. This study examines the effect of sterilization treatment on the percentage of contamination, browning, and shoot induction paulownia. Sterilization techniques used in the study consisted of four treatments, namely A (Dhitane M-45 0.2 g/100 mL, Agrept 80 WP 0.2 g/100 mL, Bayclin 20%, 15%, 10%), B (Dhitane M-45 2 g/100 mL, Agrept 80 WP 2 g/100 mL, Bayclin 15%, 10%, 5%), C (Dhitane M-45 0.2 g/100 mL, Agrept 80 WP 0.2 g/100 mL, So Klin 20%, 15%, 10 %), and D (Dhitane M-45 2 g/100 mL, Agrept 80 WP 2 g/100 mL, So Klin 15%, 10%, 5%). Sterilization techniques give the same effect on the percentage of contamination, browning, and shoot induction paulownia. Contamination caused by bacteria and fungi is 93.96% and 11.5% respectively. Explants without contamination and browning shows the development of axillary buds. Some of explants produced callus as a response to highly endogenouse hormone. Low concentration of auxin can induced 100% callus formation at the stage of multiplication
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Judul: Sterilization and Induction of Kluwih Teak Callus (Tectona abludens var. lacerata) In Vitro Culture
Abstrak: Tectona abludens merupakan tanaman endemik Jawa khususnya Gunung Kidul dan Bantul DIY yang hanya ditemukan 11 individu pohon. Perkembangbiakan T. abludens memiliki permasalahan karena diduga memiliki hambatan fisik maupun fisiologis, sehingga diperlukan upaya perbanyakan dengan teknik in vitro. Permasalahan utama dalam propagasi in vitro jati kluwih adalah sterilisasi eksplan dan induksi kalus. Penelitian ini bertujuan menganalisis respon waktu perendaman merkuri klorida (HgCl2) terhadap sterilisasi eksplan dan menganalisis respon kombinasi Zat Pengatur Tumbuh (ZPT) 2,4-D dan kinetin terhadap pembentukan kalus embriogenik. Metode analisis data yang digunakan adalah Rancangan Acak Lengkap (RAL) untuk sterilisasi eksplan dan Rancangan Acak Lengkap Faktorial (RALF) untuk induksi kalus. Hasil penelitian sterilisasi ekplan menunjukkan perlakuan perendaman 300 mg/l HgCl2 selama 15 menit menghasilkan eksplan steril tertinggi 90% pada 15 HST. Hasil penelitian induksi kalus menunjukkan perlakuan 0 ppm 2,4-D yang dikombinasikan 1,5 ppm kinetin menghasilkan persentase kalus tertinggi 73,33% pada 12 MST., Tectona abludens is an endemic plant of Java, especially Gunung Kidul and Bantul DIYwhich only found 11 individual trees. The breeding of T. abludens has problems because it is thought to have physical and physiological obstacles, so propagation efforts are needed with in vitro techniques. The main problems with in vitro techniques are explant sterilization and callus induction. This study aims to analyze the response of mercury chloride (HgCl2) immersion time to explant sterilization and analyze the response of a combination of 2,4-D Plant Growth Regulators (PGR) and kinetin to embryogenic callus formation. The data analysis methods used were Complete Randomized Design (CRD) for explant sterilization and Factorial Complete Random Design (FCRD) for callus induction. The results of the explant sterilization study showed that soaking treatment of 300 mg /l HgCl2 for 15 minutes resulted in the highest sterile explant of 90% at 15 HST. The results of callus induction research showed that 0 ppm 2,4-D treatment combined with 1.5 ppm kinetin resulted in the highest callus percentage of 73.33% at 12 MST.
Keyword: callus induction, in vitro, sterilization, Tectona abludens

Judul: Formulasi Hamiltonian untuk Menggambarkan Gerak Gelombang Soliter Dimensi Tiga di Permukaan Laut
Abstrak: Gelombang permukaan adalah gelombang yang terjadi perbedaan rapat massa antara air dan udara. Salah satu gelombang permukaan yang ditinjau adalah gelombang soliter. Formulasi gelombang soliter dilakukan dengan menggunakan formulasi Hamiltonian.
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