Judul: Analisis Molekuler dan Fenotipe Galur Padi Transgenik IR64 Generasi T3 yang Mengandung Penanda Aktivasi untuk Toleransi Kekeringan
Abstrak: Activation tagging transgenic rice cv. IR64 had been developed by introducing a construct of activation tagging transposon Ac/Ds. Phenotipic character associated with drought stress tolerance of the transgenic rice lines have not been analyzed. This study aimed to evaluate T3 generation of activation tagging transgenic rice lines cv. IR64 to drought stress treatment. The methodology of study consisted of several steps including germination of samples seeds, screening the seedling with drought strees treatment, molecular analysis of the rice lines identify hpt and bar genes, and observation of agronomic character. Results of study showed that there were 34 out of 84 plants that tolerant to drought stress. A total of 23 plants of tolerant plants were detected carrying only bar gene after PCR analysis and indicated that the plants were stable mutants. Generaly, compared to the non-transgenic plants, there were changes in several agronomic parameters of transgenic rice plants cv. IR64 T3 generation. A total of nine plants increased of its height, six plants had a faster harvesting time, and 21 plants had higher weight of 100 seeds compared by non-transgenic plants.
Keyword: Bogor Agricultural University (IPB), drought stress tolerance, activation tagged, Rice (Oryza sativa L.)

Judul: Karakterisasi Molekuler Tanaman Padi Nipponbare Transgenik 35S::OsERA1 yang Toleran Kekeringan
Abstrak: Drought is one of the factors that resulted in the growth and development of rice plants inhibited. One way to overcome this problem is use of transgenic rice plants that are tolerant to drought. The success of the transformation using OsERA1 gene in Nipponbare genotype rice requires further confirmation of tolerance to drought stress. The aim of this study was to obtain transgenic Nipponbare genotype rice contain gene 35S::OsERA1 drought tolerant, know the number of gene copies, and compare the contents and empty seeds with control. The evaluation of drought the tolerance was performed using Standard System Evaluation for Rice method (IRRI 1996). All tested transgenic genotypes were tolerant to drought. Molecular characterization using southern hybridization showed that transgenic rice plants had 1-3 copies of transgen. Drought stress inhibited plant growth, which lowers the amount of seed and increasing the void content. 18.5.4 and 6.4.10 genotypes are considered tolerant to drouht. 18.5.4 genotype have the number of grains per panicle contents of 36 seeds and 6.4.10 genotype have a 38.03% percentage of empty seeds
Keyword: transgenic plant, drought, southern hybridization, OsERA1 gene

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
Keyword: