Judul: Pengaruh Paparan Informasi, Kecukupan Diri, dan Kesadaran Hak Konsumen terhadap Kemandirian Konsumen dan Konsumerisme pada Generasi Y.
Abstrak: Konsumen di Indonesia cenderung bersikap pasrah ketika mengalami ketidakpuasan sehingga diperlukannya kemandirian konsumen serta upaya untuk memperjuangkan hak-haknya sebagai konsumen melalui konsumerisme. Penelitian ini bertujuan untuk menganalisis pengaruh paparan informasi, kecukupan diri, dan kesadaran hak konsumen terhadap kemandirian konsumen dan konsumerisme pada generasi Y. Desain penelitian menggunakan cross sectional study dengan metode online survei. Responden penelitian adalah Generasi Y dengan jumlah sebanyak 300 orang dipilih secara non probability sampling. Analisis data menggunakan analisis deskriptif dan Strucural Equation Modelling (SEM). Hasil penelitian menunjukkan terdapat pengaruh yang positif signifikan antara kesadaran hak konsumen terhadap kemandirian konsumen, kecukupan diri terhadap kemandirian konsumen, dan kemandirian konsumen terhadap konsumerisme. Sementara itu, paparan informasi tidak berpengaruh signifikan terhadap kesadaran hak konsumen dan kecukupan diri terhadap konsumerisme.
Keyword: Kecukupan diri, kemandirian konsumen, kesadaran hak konsumen, konsumerisme, paparan informasi, SEM

Judul: Pengaruh Paparan Informasi, Kepercayaan Diri, dan Kesadaran Hak Konsumen terhadap Kemandirian Konsumen dan Konsumerisme pada Generasi Y
Abstrak: Era globalisasi dan perdagangan bebas membuat tidak sedikit konsumen dijadikan sebagai objek aktivitas bisnis oleh pelaku usaha untuk mendapatkan keuntungan yang besar. Konsumen di Indonesia cenderung bersikap pasrah ketika menghadapi suatu permasalahan, dan konsumen yang berani mengadu (komplain) jumlahnya masih sangat sedikit, sehingga diperlukannya kemandirian konsumen. Generasi Y merupakan generasi yang dapat dikatakan konsumtif. Penelitian ini bertujuan untuk untuk menganalisis pengaruh paparan informasi, kepercayaan diri, dan kesadaran hak konsumen terhadap kemandirian konsumen serta konsumerisme pada generasi Y di Indonesia. Penelitian ini menggunakan desain cross sectional study dengan metode survey online. Pemilihan contoh pada penelitian ini dipilih secara non probability sampling dengan contoh yaitu konsumen yang berusia 18 sampai 36 tahun pada saat penelitian ini berlangsung. Contoh pada penelitian ini berjumlah 300 orang. Analisis data menggunakan metode SEM melalui program Analisis of Moment Structures (AMOS). Hasil penelitian ini menunjukkan kepercayaan diri memengaruhi kemandirian konsumen dan kemandirian konsumen memengaruhi konsumerisme.
Keyword: kepercayaan diri, kemandirian konsumen, kesadaran hak konsumen, konsumerisme, paparan informasi

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable