Judul: Polimorfisme Sekuens D-Loop DNA Mitokondria Pada Populasi Kajang, Mandar, Dan Dayak Benuaq
Abstrak: DNA mijokondria (mtl1NA) tnerupakan DNA sirkular, lcrsusun oleh 16569 pasang hasa yang I11CIlYUlldi 13 polipeptida sebagian Hllllai respirasi, 2 rRNA, dan 22 tRNA. Daerah D-/oop yang merupakan ~alu-satllnya daerah yang tidak mellyandi gen memiliki iaju IImlasi lebih tinggi dibandingkan hagiall lain paJa mtDNA. Analisis mtDNA banyak digunakan dalam peneiitian migrasi dan penyebaran 111<1I1U513 knrcn3 eifi mtDNA yang liJak mellgalam~ rekomhinasi, ditunmkan secara maternal, memiliki taiu 111111asi yang linggi, dan kuantitas yang linggi dalam scI. Peneiitian inl merupakan bag ian dari pcnelitian hcsar mengcnai kcragaman genom bcrbagai pnplilasi etnik Nusantara, Icbih khusus meneliti kcragallllln gClletik mtDNA pada populasi Kajang, Mandar, (Ian Dayak Bcnllaq dalam kaitannya dengan kCl1nik,U\ mgam flora dan lil11lla £Ii sckilar GaI'is Wallace. SckuclIs dacmh hipervariahcl-I (IIVR-1) D-lollfJ mtIJNA pada posisi lluklcotida (nl) 1610 1-16569 dan ntl-50 dal i masing-Illasing 30 individll populasi Kajang, Mandar, dan Dayak Benuaq le1ah dirullut, diannhsis, I1nn dib.IIl(\ingkan dt'l1gan sekuens referensi Cambridge. Total kI"-90 sampei 1llenlll~iukkan \rhlh lingginya rrckllensi l1ansi.~i (\aripatia tmnsversi, dnn \ebih tingginya tHIIlSisi anlarpirimidin (llukkot i(la (. ke T alau scbnliknya) dal ip:1(la hansisi antarpllrin (A ke Galan sebaliknya). Variasi rada 56 .~itu" po1imorfik mellghasllkan 70 haplotipc dellgan 56 silus polilllmtik yang kenUldian diidcntil1kasi tCl'hagi kc dalam 4 haplogrup spcsifik Asia: M (54.4%), B (13.3%), F (8.9%), dan BM (8.9%), sedangkan I J lmplutipe (14.4%) lainnya belum dapat diidentifikasi. Allalisis filogenetik menggunakan melode Ilci1!fI"or:joilling menunjukkan kecenderungan
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Judul: Analisis keragaman D-Loop Dna mitokondria mencit rumah (Mus Musculus Castaneus) di daerah Jakarta, Bandung dan Surabaya dengan Pcr-Rflp
Abstrak: House mice ( Mus musculus) belongs to class Mammal, infraclass Eutheria, Order Rodentia, suborder Myomorpha and family Muridae. This spesies divided into subspesies subspesies Mus musculus domesticus, Mus musculus musculus, Mus musculus castaneus and Mus musculus bactrianus (Silver 1995). Subspesies which spread all over Indonesia is Mus musculus castaneus. Mitochondrial DNA (mtDNA) is often used as genetic marker to study genetic diversity. Genetic diversity of mtDNA can be obtained using PCR-RFLP. The Primers used for analysis were CTDF and CTDR. The restriction enzyme used were HaeIII, AluI and RsaI. The samples were from Jakarta (10 mice), Bandung (11 mice) and Surabaya (9 mice) so the total amount of samples were 30 mice. We generated pcr product 1384 bp in length using PCR-RFLP. Nine haplotype were found. In Jakarta population there were three haplotype, Bandung seven haplotype and Surabaya five haplotype. Local haplotypes were found in Bandung (H-5 and H-7) and Surabaya (H-8 and H-9). The H-3 was common haplotype that were found in all population.
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Judul: Design model of early warning system for frying oil trading
Abstrak: Indonesia’ economic crisis was occurred in the period of 1997-1998, where its currency, had been fallen down more than sixth time for managing frying oil goods in the year of 1997 – 1998. During this period, the frying oil price was risen more than triple and many industries had been closed down. The government policies was issued to control frying oil prices trough trading and investment regulation, but unfortunately not effective yet. It is than realized the importance of establishing crisis management; particularly to setting up the early warning system for managing essential goods.
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