Judul: Preferensi Iklan dan Produk Serta Hubungannya dengan Konsumsi Fast Food dan Mie Instan pada Remaja Siswa SMU Negeri 1 Bogor
Abstrak: Penelitian ini secara umum bertujuan untuk mengetahui preferensi iklan dan produk serta hubungannya dengan konsumsi fast food dan mie instan pada remaja. Penelitian ini dilakukan di SMU Negeri 1 Bogor, Kota Bogor, Jawa Barat, dengan responden sebanyak 298 orang yang merupakan siswa-siswi SMU Negeri 1 Bogor kelas 1 dan 2. Lokasi penelitian ditentukan secara purposive, dengan desain penelitian cross-sectional study.
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Judul: Faktor-faktor yang Berhubungan dengan Konsumsi Fast Food Waralaba Modern dan Tradisional pada Remaja Siswi SMU Negeri di Jakarta Selatan
Abstrak: Penelitian ini secara umum bertujuan untuk mengetahui konsumsi fast food waralaba modern dan tradisional serta faktor-faktor yang berhubungan dengan konsumsi fast food waralaba pada remaja siswa SMU Negeri di Jakarta Selatan. Penelitian ini dilakukan di SMU Negeri 70 dan SMU Negeri 32 Jakarta Selatan dimana sebelumnya dilakukan survei awal guna mengetahui responden yang mengkonsumsi fast food dan restoran yang banyak dikunjungi responden dalam sebulan terakhir. Lokasi penelitian ini ditentukan secara purposive dengan desain penelitian cross-sectional study. Sedangkan contoh dalam penelitian ini adalah siswa-siswi kelas I dan II dari kedua SMU Negeri tersebut yang berjumlah 81 responden.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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