Judul: Efek Genistein terhadap Derajat Alergi dan Kadar Imunoglobulin E Marmut yang Diinduksi Putih Telur
Abstrak: Research about the effect of genistein on immunoglobulin E level has been done by using guinea pig as the murine model. Guinea pigs were divided into two groups namely control and genistein. Control group were not sensitisized, while genistein were sensitisized for 40 days by sub cutaneous injection of egg white, NaCl 0.9%, and Al(OH)3 for 30 days, continued by induced egg white in drinking water between day 31 until day 39. On day 40th all these guinea pig were injected with serum in dorsal surface. Day 42nd control group were induced orally with 1 mL aquadest, while genistein group induced orally with 1.84 μg/100 g guinea pig’s body weight in 1 mL aquadest. All guinea pigs then induced with egg white as the allergen and Evan’s Blue dye intravenously. Blood sample were taken before sensitization and after induction of egg white. Immunoglobulin E level of blood sample was calculated by ELISA method, allergic degree were measured by Pasive Cutaneous Anaphylaxis (PCA). PCA analysis shown that control group and genistein group has the equal bluish intensity. IgE level also support the PCA data, thus, it can not be stated that genistein induction alter the decrease of immunoglobulin E level.
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Judul: Pengaruh Kuersetin terhadap Derajat Alergi dan Kadar Imunoglobulin E pada Marmot yang Diinduksi Putih Telur
Abstrak: Quercetin is one of flavonoid class which has been clinically proven for allergic and also has been syntheticed. It has not researched yet to determine the smallest which can effect as antiallergic. The guinea pigs divided into two groups, control (K) and Quercetin (Q) groups. Each groups were comprised three guinea pigs and all of it were adapted for 14 days. Guinea pigs were sensitized for 39 days with egg white 2 mg/mL dissolved by Al(OH)3 as adjuvant and buffer saline by forced egg white down. While, another three guinea pigs were adapted for 3 days to determine pharmacologic dosage based Lethal Dose 50 (LD50) of Quercetin. The result of this assay showed that at dose 13.92 mg/kg body weight is safe dosage. This dosage then used for determine the effect of quercetin as antiallergy with Evans blue dye as indicator (AKP method). The result of AKP showed that quercetin has no significant effect. The concentration of Immunoglobulin E (IgE) in guinea pigs serum also same for all guinea pigs < 2.4 ng/mL. The result of this research was quercetin at dose 13.92 mg/kg body weight has no significant effect as antiallergic.
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Judul: Cycle Hamilton pada Graf Lengkap, Graf Regular, dan Graf 2-Connected 4-Regular Berorder Kurang dari Sepuluh
Abstrak: Suatu graf disebut graf Hamilton apabila graf tersebut memuat cycle Hamilton, yaitu cycle yang melewati setiap vertex tepat satu kali. Menentukan keberadaan cycle Hamilton pada suatu graf dapat menggunakan syarat cukup yang berupa teorema-teorema yang telah diperkenalkan sejak tahun 1950-an. Beberapa syarat cukup yang dibahas dalam karya ilmiah ini adalah syarat cukup yang berhubungan dengan graf lengkap dan graf regular. Suatu graf lengkap berorder 𝑛������ ≥ 3 dan graf r-regular berorder 𝑛������ ≤ 2𝑟������ + 1 merupakan graf Hamilton, Karena kedua graf tersebut memuat cycle Hamilton. Selanjutnya, syarat cukup tersebut digunakan untuk menentukan keberadaan cycle Hamilton pada graf 2-connected 4- regular berorder kurang dari 10.
Keyword: graf Hamilton, graf regular, graf lengkap, graf 2-connected 4-regular