Judul: Cloning Method of Pest Resistance Gene from Sengon (Paraserianthes falcataria).
Abstrak: Sengon is one of fast growing trees species, which is widely cultivated in plantations forest in Indonesia due to its multipurpose nature, and easy to grow. The plantation, however, faces serious problem of stem borer (Xystrocera festiva) attack, which was reported could destroyed sengon plantation. Meanwhile, several studies have reported the role of trypsin inhibitors (TI) and alpha-amylase inhibitor (AAI) in resistance in trees to insects. This research aimed at cloning a possible pest resistance gene from sengon. Sengon genomic DNA was isolated using CTAB buffer combined with binding columnn of GenElute plant Genomic DNA Miniprep Kit. Designed primer pairs did not generate amplicon. PCR products were obtained from published degenerate primers as two amplicons, with predicted size of 350 bp and 550 bp for AAI gene, and 600 bp and 800 bp for TI gene. Cloning with pGEM-T Easy vector resulted in several white colonies. Sequencing from selective colonies with long and short amplicon of AAI and TI sequences with 693 nucleotide, 278 nucleotide and 832 nucleotide, 278 nucleotide, respectively. BLASTN and BLASTX the sequences did not confirm that the inserts were AAI and TI genes. Further research is still needed to identify pest resistance gene in sengon tree.
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Judul: Pengembangan Metode Kloning Gen Penyandi Trypsin Inhibitor Sengon (Falcataria moluccana Miq.)
Abstrak: Kendala utama pengembangan hutan tanaman sengon (Falcataria moluccana Miq.) adalah serangan hama boktor (Xystrocera festiva). Secara alami, sengon memiliki mekanisme pertahanan biokimia terhadap boktor, salah satunya yaitu trypsin inhibitor (TI). Penelitian ini bertujuan untuk mengembangkan metode untuk isolasi dan kloning DNA penyandi TI asal tanaman sengon. Percobaan meliputi tiga tahap yaitu ekstraksi DNA sengon, Polymerase Chain Reaction (PCR) dengan primer penyandi TI dari tanaman lain, dan kloning hasil PCR. Ekstraksi DNA dari daun sengon dilakukan dengan metode CTAB I, CTAB II dengan modifikasi kit Purelink®, kit Purelink® Plant Total DNA purification, dan Qiagen® DNeasy plant miniprep kit yang ditambahkan PVP 26%. Hasil terbaik dari Qiagen® DNeasy plant miniprep kit dengan konsentrasi DNA 100 ng/μL. Reaksi PCR dilakukan dengan GoTaq® PCR Core Systems, PCR Supermix Invitrogen, GoTaq® DNA Polymerase, dan Platinum® Taq DNA Polymerase, dan yang berhasil memunculkan pita amplikon PCR menggunakan primer BTIw1 yaitu GoTaq® PCR Core Systems dengan konsentrasi hasil PCR 438 ng/μL. Kloning dilakukan dengan tiga metode yaitu heat shock CaCl2, heat shock Transformation buffer, dan Thermoscientific® InsTAclone PCR cloni
Keyword: cloning, method, Polymerase Chain Reaction (PCR), sengon, trypsin inhibitor

Judul: Persamaan Korteweg-de Vries Orde Tinggi untuk Menggambarkan Gerak Gelombang Internal
Abstrak: The ocean can be considered of layers of fluid that each layer has a constant density. Internal waves are waves occur in the interface of every'two layers with different density. Because it occures underneath the surface of the ocean, it is invisible. One of internal waves is internal solitary waves. One of formulation that can describe intemal waves movement is KdV equation. KdV equation is derived from governing equations of ideal fluid in Lagrange formulation using asymptotic method. On this thesis, KdV equation is derived in higher order and then its solution is derived by using internal solitary waves assumption. An example of a two-layer fluid is a case will be used. By using Mathematica6 software, for this case will be obtained the coefficients in lower and higher order explicitly and the corresponding between coefficients and relative lower layer depths and fluid depths, the corresponding between coefficients and relative densities, and also observed the different between solitary waves in lower and higher order.
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