Judul: Diferensiasi leukosit Mencit (Mus musculus) yang diberi pretreatment pada penyiapan hewan untuk penelitian biomedis Pembimbing Estuningsih, Sri;Esfandiari, Anita
Abstrak: Di Indonesia, penyediaan hewan percobaan yang memenuhi syarat masih sedikit dan secara nominal tergolong relatif mahal. Oleh karena itu perlu dilakukan suatu tindakan untuk mendapatkan hewan percobaan yang berkualitas baik. Salah satu usaha untuk mendapatkan hewan dengan kualitas yang lebih baik adalah melakukan tindakan pretreatment. Tujuan dari penelitian ini adalah untuk melihat gambaran leukosit mencit (Mus musculus) yang diberi obat-obatan pretreatment meliputi anthelmentik, antibiotika dan antiprotozoa untuk penyiapan hewan percobaan agar kualitasnya lebih baik. Penelitian ini menggunakan 36 ekor mencit (Mus musculus) masing-masing 18 ekor mencit jantan dan 18 ekor mencit betina dengan umur 34 minggu saat diberi pretreatment. Mencit dibagi menjadi 6 kelompok berdasarkan perlakuan pemberian obat yaitu kelompok 1 diberi anthelmentik saja, kelompok 2 diberi anthelmentik dan antibiotika, kelompok 3 diberi anthelmentik dan antiprotozoa, kelompok 4 diberi anthelmentik, antibiotika dan antiprotozoa, kelompok 5 mencit alas kain tanpa pemberian obat-obatan pretreatment dan kelompok 6 mencit alas serutan kayu tanpa pemberian obatobatan pretreatment. Darah dikoleksi sebelum dan sesudah pemberian obat-obatan pretreatment. Hasil diamati dibawah mikroskop cahaya dengan perbesaran 10x100 dan dengan bantuan software Digital Eyepiece Camera. Hasil yang didapatkan tidak ada perbedaan yang signifikan (p<0,05) antara kontrol alas kain dengan masing-masing kelompok perlakuan. Terjadi peningkatan dan penurunan masing-masing jenis sel leukosit namun hal tersebut masih dalam interval normal. Secara umum pemberian obat-obatan pretreatment dilihat dari diferensial leukositnya lebih baik dibandingkan dengan mencit konvensional, terutama pada kelompok pemberian anthelmentik, antibiotika dan antiprotozoa yang menunjukkan hasil lebih baik.
Keyword: leukocyte, mice, pretreatment

Judul: Gambaran Diferensial Leukosit Mencit (Mus Musculus) Setelah Pemberian Berbagai Dosis Nano Logam Besi (Fe) Secara Intraperitonial
Abstrak: Besi dalam tubuh berperan penting dalam pembentukan sel darah merah dan bagian dari enzim hemoprotein. Penelitian ini bertujuan untuk mengkaji pengaruh pemberian nano logam besi (Fe) terhadap persentase differensial leukosit mencit (Mus musculus). Hewan coba yang digunakan dalam penelitian ini sebanyak 30 ekor mencit berumur 10 minggu dan diinjeksikan secara intraperitonial larutan logam Fe. Mencit dibagi dalam 6 kelompok yaitu kontrol negatif (tidak diberi logam Fe), logam Fe dosis 8,3 mg/mL, logam Fe dosis 24,8 mg/mL, logam Fe dosis 41,25 mg/mL, logam Fe dosis 57,8 mg/mL dan logam Fe dosis 74,3 mg/mL. Hasil penelitian menunjukkan rata-rata persentase neutrofil, eosinofil dan monosit mengalami peningkatan. Persentase basofil rata-rata berada dalam rentang normal, sedangkan persentase limfosit cenderung menurun dibandingkan sebelum perlakuan. Pemberian nano logam Fe memberikan pengaruh secara signifikan terhadap gambaran leukosit darah mencit.
Keyword: diferensial leukosit, nano logam besi, mencit

Judul: Characteristic of Overshooting Top (OT) Based on  Himawari  8  Satellite  as  a  Convective  Cloud  Feature  that  Generates  Extreme Weather in the Java Island Region
Abstrak: Overshooting Top (OT) is a feature at the top of a convective cloud that is associated with extreme weather. Research on OT in tropical Indonesia is still very limited. The existence of geostationary satellites with wide area coverage and fast frequency observations is useful for observing the fast dynamics of the OT life cycle. Therefore, this study intends to analyze the relationship between OT and extreme weather, updraft and thermodynamic conditions of OT when extreme weather occurs, the best objective method for detecting OT in the day time and night time, and the spatiotemporal distribution of OT in the tropical region of Java Island during the 2018-2021 period. Based on visual analysis using visible channel and Himawari 8 sandwich products, 87% of extreme weather events in Java were accompanied by the appearance of OT. It indicates that the presence of the OT pattern is a convection cloud pattern or feature that is closely related to extreme weather events for the Java Island region. The existence of a time lag between the appearance of OT and extreme weather events on Java Island indicates that the OT of convection cloud can be used as a precursor or nowcasting for the appearance of extreme weather. Most of the convective cloud OT features during extreme weather events on Java Island have altitude that were only able to exceed the altitude of the equilibrium level and few were able to exceed the tropopause level of 9 km – 16 km. The difference in atmospheric conditions during extreme weather events on Java Island, which is in the tropics and subtropics, is a strong and unstable convective energy condition characterized by a larger CAPE, as well as unstable KI, LI, SI and TTI values. It is offset by kinematic factors in the form of a weak vertical wind shear (bulk wind shear 0-6 km) and a higher freezing point height. The difference between extreme weather events in the Java Island region and other tropical regions compared to subtropical regions is the strong updraft speed of convection clouds. The IRW Texture COMB algorithm based on the IRW-Texture Gradient modification and the COMB algorithm shows a significant increase in quality for detecting OT and non-OT pixels in Java. Rainy days with rainfall > 50 mm/day are very common in areas with an altitude of > 500 m. On the other hand, the level of convection activity which is indicated by the presence of OT and lightning is significantly reduced in this area. OT of convective clouds appear more frequently during the DJF season and less frequently during the JJA season. OT often appears during the day time and rarely at night time. Most OTs appear in the central part, away from the south and north coasts of Java Island during the day, while OTs that appear near the south and north coasts of Java Island can appear in the morning or afternoon.
Keyword: convective clouds, extreme weather, Overshooting Tops, Himawari 8 Satellite