Judul: Karakterisasi Protein Antigenik Larva 3 Gnathostoma spinigerum pada Ikan Belut Rawa (Monopterus alba) Menggunakan Teknik Immunoblotting
Abstrak: Gnathostoma spinigerum is an intestine nematode in carnivore but can cause human gnathostomiasis. Man is a paratenic host and can acquires the infection by eating non cooked or inadequately processed intermediate hosts, e.g. freshwater fishes, frogs, birds and snakeswhich contain encapsulated third stage larvae. Third stage larvaes (L3s) of G. spinigerum were most found in liver and muscle of swamp eels (Monopterus alba). The objective of this research was to analyze the specific antigens of excretory-secretory (ES) products of G. spinigerum L3s (ES-Ag Gs L3) against rabbit immunoglobulin G (IgG) polyclonal antibody-anti ES-Ag Gs L3s using Western blotting technique. The result of this research was hoped to be a basic for the development of immunodiagnostic and molecular diagnostic of gnathostomias. Excretory-secratory proteins isolation of G. spinigerum L3 were obtained by incubating L3 in RPMI 1640 medium which contains 100 units/ml penicillin G and 100 μg/ml streptomycin for four days at 37°C under 5% CO2 in air. The crude antigen protein of ES-L3-Gs were not purificated. Immunoglobulin G anti ES-Ag Gs L3s were obtained by immunize New Zealand strain white rabbits with ES-Ag Gs L3s. Bands with apparent molecular weights of 12.6, 18.6, 37.9, 41.7 and 49.5 kDa were identified from immunoblotting reactions between IgG-anti ES-Ag Gs L3s and ES-Ag Gs L3s. The proteins of ES from other nematode larvae with apparent molecular weights of 12.6, 18.6, and 37.9 were also recognized by IgG-anti ES-Ag Gs L3s. The specific proteins with molecular weights of 41.7 and 49.5 kDa were potential antigens for immunodiagnostic and molecular diagnostic of gnathostomiasis in swamp eels and human gnathostomiasis in Indonesia.
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Judul: Isolasi dan karakterisasi protein ikan tongkol (Auxis thazard), kerang hijau (Perna viridis) dan udang jerbung (Penaeus merguiensis) untuk pembuatan isolat alergen
Abstrak: Seafoods including fish, crustaceans and shellfish play an important role in human nutrition. However, it is also recognised as an important cause of food allergies, especially in coastal countries, including Indonesia. Treatments of these diseases still rely on avoiding the sources of the alergens, however it can also cause malnutrition which is also dangerous to health. To determine the specific food protein causing allergic reactions, cutie test was performed using isolate food protein. Until the present time, Indonesia still imports kits containing isolate proteins for food allergy diagnosis, therefore effort to locally produce the kit will reduce health cost for patients with allergic diseases. The objective of this study was to produce seafoods protein extract that is potential to be used as isolate of allergens for food allergy diagnosis. Two extract sarcoplasmic and myofibril protein were obtained from fish, shrimp and mussel by extraction in phosphate buffer with diference ionic strength Protein profile were then detected by means of sodium dodecyl polyacrylamide gel electrophoresis (SDS-PAGE). IgE binding pattern was analyzed by using ELISA and immunoblotting using sera from 20 respondents with histories of food allergy. The result of the study showed that samples of seafood extract protein consist of several protein with various molecular weight in the range about 14 to 143 kDa. The component were identified as tropomyosin (34-38 kDa), parvalbumin (~12 kDa), myosin light chain (~17.5 kDa) and arginin kinase (40 kDa) which are major allergens in seafoods. Further analysis with ELISA showed that the seafood protein extracts contained several IgE-binding proteins. Immunoblot of three extract samples from seafoods showed that components identified as allergens to each allergic subject have different molecular weight. The results demonstrated that crude extract could be directly used as isolate allergen for diagnostic test allergy (skin prick test) without isolating the major allergen components.
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Judul: Konsep dan kebijaksaan ruang terbuka bumi serpong damai
Abstrak: Pembangunan yang berlangsung cepat di Jakarta, seringkali menyita ruang-ruang terbuka yang ada. Kebijaksanaan pemerintah dalam mengatur ruang terbuka suatu wilayah, tidak selalu dijalankan sepenuhnya. Kota Baru Bumi Serpong Damai dibangun untuk mengatasi beban kota Jakarta dalam memenuhi kebutuhan penduduknya secara menyeluruh; oleh karena itu, Kota Baru Bumi Serpong Damai disebut sebagai kota mandiri. Keberadaan kota mandiri Bumi Serpong Damai bertujuan untuk mewujudkan suatu kota baru swasembada, serta membentuk suatu kota baru yang memberikan citra kota abad 21. Bentuk skenario kota yang dipilih adalah Skenario Inti Perkotaan. Skenario Inti Perkotaan menempatkan pusat kegiatan berupa Central Bussiness District sebagai penggerak utama. Tata guna lahan Kota Baru Bumi Serpong Damai dibuat dengan memperhatikan kondisi saat ini, struktur kota yang dikehendaki dan memperhatikan kaitan antara potensi setiap bagian tapak dengan fungsi-fungsi yang akan diletakan. Ruang terbuka adalah lahan yang tidak dibangun atau lahan yang di atasnya terdapat bangunan dengan luasan kurang dari 20% dari luasan seluruh lahan. Berdasarkan Instruksi Menteri Dalam Negeri No 14/1988, suatu wilayah seharusnya memiliki ruang terbuka dan ruang terbangun de- ngan perbandingan 40%:60%. Prinsip ruang terbuka Bumi Serpong Damai adalah bahwa ruang terbuka merupakan barang mahal, sehingga harus dimanfaatkan seefisien dan seefektif mungkin. Ruang Terbuka Bumi Serpong Damai ditetapkan dengan mempertimbangkan kebutuhan-kebutuhan masyarakat, kemampuan dalam pembiayaan pembangunan, pengelolaan dan pemeliharaan. Kebijaksanaan Ruang Terbuka Bumi Serpong Damai dengan ruang terbangun adalah perbandingan 40%:60%...
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