Judul: Pengembangan Metode Isolasi Total RNA, mRNA dan Sintesis cDNA Sengon (Falcataria moluccana L. Nielsen) yang Sehat dan Terserang Hama Boktor (Xystrocera Festiva).
Abstrak: Sengon (Falcataria moluccana L. Nielsen) has a natural self-defense mechanism towards pest infection by synthesizing certain macromolecul such as tripsin inhibitor and alfa-amilase inhibitor. Characterization of those genes which produce the inhibitor need to be done on resistant trees and the vulnerable ones, to define the differences so that can be used for tree improvement. This research aim to develop an appropriate isolation methods of total RNA, mRNA and cDNA of plants that have a inhibitor enzymes such as sengon. The materials used in this research were cambium and timber tissue from resistant and vulnerable tree. Total RNA was isolated using Total RNA Mini Kit by Geneaid, mRNA was isolated using PolyATract mRNA Isolation System III with Magnetic Stand by Promega and cDNA synthesize was conducted using cDNA Substraction Kit by Clontech and RevertAid RT Transcription Kit by Thermo. The highest concentration of total RNA isolation were 65,60ng/μl from modification methods, and the best mRNA yields was resulted by modification methods. The highest concentration for cDNA synthesize were 2296,17ng/μl from modification methods.
Keyword: sengon, RNA and mRNA isolation, cDNA synthesize

Judul: α-Amylase Inhibitor Gene Expression of Healthy Sengon (Falcataria moluccana Miq) Healthy and Attacked by Boktor Pests
Abstrak: Sengon (Falcataria moluccana Miq) merupakan salah satu pohon pionir dan memiliki berbagai macam kegunaan. Penanaman sengon secara monokultur menimbulkan terjadinya ledakan hama penggerek batang atau boktor (Xystrocera festiva) yang merusak kayu dan menjatuhkan nilai jual batang sengon. Studi molekuler aktivitas gen α-amilase inhibitor (AAI), yang merupakan salah satu gen ketahanan terhadap hama boktor sangat mendukung program pemuliaan sengon. Penelitian ini bertujuan mengkuantifikasi tingkat ekspresi gen α-amilase inhibitor sengon yang sehat dan terserang hama boktor. Prosedur penelitian ini meliputi isolasi RNA total, sintesis cDNA, perancangan primer spesifik gen AAI (target) dan aktin (kontrol), optimasi suhu annealing primer gen AAI dan aktin, ekspresi gen AAI dan aktin pada Real Time PCR dan analisis data. Fragmen cDNA teramplifikasi pada suhu annealing 61,7oC untuk gen aktin dan 61oC untuk gen AAI. Tingkat ekspresi gen AAI 2 pada pohon sengon yang terserang hama boktor memiliki nilai yang lebih tinggi yaitu 55,60 pada sampel 1 dan 6,96 pada sampel 3.
Keyword: Falcataria moluccana, α-Amilase Inhibitor, Real Time PCR

Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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