Judul: Validasi metode ekstraksi DNA pada analisis DNA babi dalam produk bakso
Abstrak: There are many cases of fraud meat-based food products found in our society. Polymerase chain reaction (PCR) technique has been developed as a precise and quick method to identify meat species both from raw meat and from meat products. Extraction is the critical level on PCR analysis because it was very related with the quality of the isolated DNA. Therefore, validation of extraction method was carried out in this research to ensure that the extraction results had good DNA qualities. The validated extraction method was then tested for its sensitivity to detect the contamination of pork meat in meatball products made with various pork contamination and also in meatball products sold in Bogor. The validation results showed that the DNA extraction method had detection limit of DNA purity of 0.117, quantitation limit of DNA purity of 0.389, relative standard deviation of 2.31%, and uncertainty of DNA purity of (1.93 ± 0.04). The pork contamination could still be detected up to 0.5% (w/w) contamination and no pork contamination found in meatball samples from 3 market in Bogor (Pasar Anyar, Pasar Bogor, and Pasar Warung Jambu)
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Judul: Aplikasi Penggunaan Gen Sitokrom B Dengan Teknik Polymerase Chain Reaction (PCR) Sebagai Pendeteksi Campuran Daging Tikus Pada Produk Bakso
Abstrak: Polymerase chain reaction (PCR) technique was developed as a precise and quick method to identify meat species in both of raw meat and meat products. Rat primer has been developed from mitochondrial cytochrome b gene sequence. The aim of this study was to find out the ability and sensitivity level from this primer as contamination detector in meatball, and also to indentify the contamination of rat meats in meatball products which are sold in the Bogor City. Meatball samples were prepared from both mixtures rat meat and beef at different levels (1, 5, 10, 15, 20 and 25%) in meatball. As the result, rat’s meat could be identified start from 5% level and were amplified definitely at level contamination 15%. There were no rat meat found in the market samples or in other words, meatball sold in three big markets in Bogor City (Pasar Anyar, Pasar Bogor dan Pasar Jambu Dua) were free from rat contamination.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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