Judul: Penambahan Glutamat sebagai Pelindung Tekanan Osmosis dalam Kondisi Pembiakan Etanol Tinggi pada Rekombinan Escherichia coli
Abstrak: Enhancement of Escherichia coli recombinant cell growth in high ethanol culture conditions was investigated by additional glutamate as osmoprotectant. It was also supported by analysis of dry cell weight (DCW), glucose uptake, and acetate accumulation. Escherichia coli cells were grown under aerobic conditions with ethanol and glutamate addition in the culture time of t = 6 h. In high ethanol culture conditions (20 g/l ethanol) without glutamate acid additions, DCW and glucose uptake reached in 0.91±0.14 g/l and 13.23±0.89 g/l, respectively. These values were lower than culture conditions with 2 g/l glutamate acid additions (1.8±0.03 g/l DCW and 17.75±0.43 g/l glucose uptake), which also suppressed the accumulation of acetate in 3.29 g/l. Based on carbon flow in central carbon metabolism, the accumulation of acetate was suppressed by glutamate additions in the culture because the carbon flow diversion to tricarboxylic acid (TCA) cycle instead of entering the acetate. The accumulation of acetate also showed as a result of an overflow metabolism by high rate of glucose uptake and oxygenation level.
Keyword: Bogor Agricultural University (IPB), overflow metabolism, glutamate, Escherichia coli, central carbon metabolism, acetate

Judul: Penambahan glutamat sebagai pelindung tekanan osmosis dalam kondisi pembiakan etanol tinggi pada rekombinan Escherichia coli
Abstrak: Enhancement of Escherichia coli recombinant cell growth in high ethanol culture conditions was investigated by additional glutamate as osmoprotectant. It was also supported by analysis of dry cell weight (DCW), glucose uptake, and acetate accumulation. Escherichia coli cells were grown under aerobic conditions with ethanol and glutamate addition in the culture time of t = 6 h. In high ethanol culture conditions (20 g/l ethanol) without glutamate acid additions, DCW and glucose uptake reached in 0.91±0.14 g/l and 13.23±0.89 g/l, respectively. These values were lower than culture conditions with 2 g/l glutamate acid additions (1.8±0.03 g/l DCW and 17.75±0.43 g/l glucose uptake), which also suppressed the accumulation of acetate in 3.29 g/l. Based on carbon flow in central carbon metabolism, the accumulation of acetate was suppressed by glutamate additions in the culture because the carbon flow diversion to tricarboxylic acid (TCA) cycle instead of entering the acetate. The accumulation of acetate also showed as a result of an overflow metabolism by high rate of glucose uptake and oxygenation level.
Keyword: Bogor Agricultural University (IPB), overflow metabolism, glutamate, Escherichia coli, central carbon metabolism, acetate

Judul: Warna bulu kucing ras persia: variasi pola warna dan pewarisannya
Abstrak: Kucing Persia mempakan kucing rasyang paling urnum dijumpai di Indonesia. Kucing Persia memiliki bulu yang panjang dengan variasi wama bulu yang sangat beragam. Perkawinan yang lebih terarah sangat perlu dilakukan untuk meningkatkan kualitas genetik warna dan pola wama bulu kucing. Penelitian ini bertujuan untuk rnempelajari variasi warna dan pola wama bulu kucing Persia yang terdapat di Indonesia, yang dilakukan di kantor pusat Cat Fancy Indonesia mulai bulan Oktober 2000 sampai Januari 2001. Data yang digunakan rnempakan data pedigree kucing Persia yang tercatat di Cat Fancy Indonesia dari tahun 1997 sampai Oktober 2001 yang terdiri dari 187 jantan dan 258 betina, selain itu juga dilakukan pengamatan langsung kepada tiga penyayang dan breeder kucing yang ada di Bandung.
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