Judul: Landscape Design of Kebondalem Main Stadium in Kendal Regency Based on Biophilic
Abstrak: Stadion Utama Kebondalem merupakan salah satu ruang terbuka hijau yang ada di Kabupaten Kendal. Kondisi Stadion Utama Kebondalem Kendal saat ini sedang dalam proses rencana pengembangan. Penelitian ini bertujuan untuk menganalisis potensi dan permasalahan dengan pendekatan aspek fisik-biofisik dan sosial-budaya di Stadion Kebondalem Kendal sebagai panduan dalam merancang rekomendasi desain. Metode yang digunakan adalah pendekatan desain berdasarkan persepsi dan preferensi pengguna. Hasil penelitian menunjukkan bahwa tapak memiliki potensi dan kendala. Terutama pada aspek sosial budaya, tapak memiliki nilai persepsi yang kurang baik oleh pengunjung dan dianggap tidak dapat memenuhi kebutuhan aktivitas yang ada. Konsep desain yang diangkat adalah biofilik. Konsep biofilik merupakan bagian dari konsep yang mendekatkan manusia dengan alam secara lebih intim. Selain itu, desain biofilik dapat memberikan dampak bagi pengguna seperti mengurangi tingkat stress, meningkatkan produktivitas, meningkatkan kreatifitas dan kejernihan pikiran, meningkatkan kesejahteraan hingga meningkatkan laju pemulihan tubuh manusia. Desain akhir dibuat dengan pola adopsi sel tanaman dan menciptakan ruang terbuka hijau yang fungsional. Dari desain yang dibuat, menunjukan bahwa Stadion Kebondalem Kendal dengan menerapkan konsep biofilik dan melakukan penataan ruang, setidaknya dapat mewadahi 8 jenis aktivitas. Tidak hanya memiliki fungsi untuk mewadahi aktivitas olahraga, namun juga dengan mewadahi berbagai kegiatan lain dari masyarakat sekitar, seperti relaksasi, apresiasi, komersial, bermain, sosialisasi, edukasi, dan hiburan., Kebondalem Main Stadium is one of the green open spaces in Kendal City. The condition of Kebondalem Kendal Main Stadium is currently in the process of development plan. This research aims to analyze the potential and problems with the approach of physical-biophysical and socio-cultural aspects at Kebondalem Kendal Stadium as a guide in designing design recommendations. The method used is a design approach based on user perceptions and preferences. The results showed that the site has potential and constraints. Especially in the socio-cultural aspect, the site has an unfavorable perception value by visitors and is considered unable to meet the needs of existing activities. The concept raised is biophilic. The biophilic concept is part of a concept that brings humans closer to nature in a more intimate way. In addition, biophilic design can have an impact on users such as reducing stress levels, increasing productivity, increasing creativity and clarity of thought, improving well-being to increasing the rate of recovery of the human body. The final design is made with a plant cell attachment pattern and creates a functional green open space. From the design made, it shows that the Kebondalem Kendal stadium by applying the biophilic concept and structuring the space, can accommodate at least 8 types of activities. not only has a function to accommodate sports activities, but also by accommodating various other activities of the surrounding community, such as relaxation, appreciation, commercial, playing , socialization, education, and entertainment.
Keyword: Desain Lanskap, ruang terbuka hijau, biofilik, stadion

Judul: Landscape Design of Bendi Park as Outdoor  Sports and Play Area
Abstrak: Tingginya jumlah penduduk dengan padatnya bangunan di perkotaan terutama Jakarta menyebabkan tingginya kebutuhan ruang terbuka hijau dalam suatu wilayah. Taman Bendi sebagai salah satu taman di Jakarta memiliki ruang terbuka hijau yang saat ini kondisinya belum optimal dari segi fasilitas dan penggunaannya. Penelitian ini bertujuan membuat desain lanskap Taman Bendi sebagai area olahraga outdoor dan bermain di Kecamatan Kebayoran Lama dan sekitarnya. Penelitian ini dilakukan dengan menggunakan metode kualitatif dan kuantitatif. Metode kualitatif adalah penelitian yang menghasilkan data deskriptif berupa kata-kata tertulis dari orang orang dan perilaku yang dapat diamati. Dalam pendekatan kualitatif fokus terhadap aspek fisik dan biofisik. Metode analisis kuantitatif merupakan penelitian ilmiah yang sistematis terhadap bagian-bagian dan fenomena serta kualitas hubungan hubungannya. Berdasarkan pendekatan kuantitatif, data hasil kuesioner diolah menggunakan metode cross tabulation untuk mengetahui pengaruh rentang usia terhadap persepsi dan preferensi desain. Hasil penelitian ini berupa site plan dengan desain yang dapat memaksimalkan potensi dan memberikan solusi permasalahan yang ada di Taman Bendi, planting plan, gambar potongan, dan gambar perspektif., The high number of residents with the density of buildings in urban areas, especially Jakarta, causes a high need for green open space in an area. Bendi Park as one of the parks in Jakarta has green open space which is currently not optimal in terms of facilities and use. This study aims to design the landscape of Bendi Park as an outdoor sports and play area in Kebayoran Lama District and its surroundings. This research was conducted using qualitative and quantitative methods. Qualitative method is research that produces descriptive data in the form of written words from people and observable behavior. In the qualitative approach the focus is on physical and biophysical aspects. The method of quantitative analysis is a systematic scientific study of the parts and phenomena and the quality of their relationships. Based on the quantitative approach, the questionnaire data were processed using the cross tabulation method to determine the effect of age range on design perceptions and preferences. The results of this study are in the form of a site plan with a design that can maximize potential and provide solutions to problems that exist in Bendi Park, planting plan, section drawings, and perspective pictures.
Keyword: Area olahraga dan bermain, desain lanskap, ruang terbuka hijau, taman  bendi

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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