Judul: Tipologi ekosistem mangrove dan hubungannya dengan keberadaan kepiting (Brachyura) di KPH Mantingan desa Banggi kabupaten Rembang, Jawa Tengah
Abstrak: Ekosistem mangrove di KPH (Kesatuan Pemangkuan Hutan) Mantingan Banggi merupakan ekosistem mangrove yang ditanam secara swadaya oleh masyarakat. Ekosistemmangrove ini mempunyai banyak manfaat yang bisa dimanfaatkan oleh masyarakat setempat, namun pada kenyataanya belum termanfaatkan secara maksimal.
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Judul: Pengelolaan Ekosistem Mangrove Berbasis Co-Management di Desa Pasarbanggi, Kabupaten Rembang, Jawa
Abstrak: Mangrove merupakan salah satu ekosistem penting pesisir yang memiliki berbagai manfaat dan pemanfaatannya dilakukan oleh berbagai pemangku kepentingan. Penelitian ini bertujuan untuk 1) mengetahui dan menganalisis kondisi sumber daya mangrove dan masyarakat pemanfaat,2) menganalisis peran dan fungsi setiap pemangku kepentingan, serta 3) mengetahui dan menganalisis bentuk co-management terhadap pengelolaan ekosistem mangrove Desa Pasarbanggi, Kabupaten Rembang. Pengambilan data vegetasi mangrove dilakukan dengan menggunakan metode transek kuadrat; sedangkan pengambilan data sosial ekonomi, kelembagaan, dan wawancara dengan metode survei dan purposive sampling. Data yang diperoleh dari vegetasi mangrove meliputi kerapatan, frekuensi, penutupan, dan indeks nilai penting mangrove. Analisis pemangku kepentingan dengan menggunakan matriks kepentingan dan pengaruh. Penelitian menunjukkan terdapat 5 jenis mangrove di Desa Pasarbanggi yaitu Avicennia marina, Rhizophora mucronata, Rhizophora apiculata, Rhizophora stylosa dan Sonneratia alba. Strategi dalam rangka perluasan hutan mangrove yaitu dengan jenis-jenis tersebut. Analisis stakeholder mengidentifikasi 16 pemangku kepentingan yang diklasifikasi dalam 4 grup yaitu subject, key players, crowd, dan by standers. Pengelolaan mangrove di Desa Pasarbanggi masih dalam tahap konsultatif. Untuk meningkatkan efektifitas, pengelolaan diarahkan pada tingkat kooperatif dalam spektrum co-management dengan strategi manajemen.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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