Judul: Karakteristik Habitat Kuskus beruang (Ailurops ursinus Temminck, 1824) di Taman Nasional Bantimurung Bulusaraung, Sulawesi Selatan.
Abstrak: Kuskus beruang (Ailurops ursinus) adalah satwa endemik yang tergolong vulnerable. Taman Nasional Bantimurung Bulusaraung khususnya Resort Balocci merupakan salah satu habitat kuskus beruang. Penelitian ini bertujuan untuk menganalisis karakteristik habitat kuskus beruang. Karakteristik habitat kuskus beruang diperoleh melalui pengamatan dengan metode Ad libitum sampling, pengamatan langsung dan wawancara. Hasil penelitian menunjukkan bahwa kuskus beruang hidup pada wilayah dengan ketinggian 664 – 1,015 mdpl, suhu 26 - 29 oC, dan kelembaban udara 70 – 82%. Indeks Nilai Penting tertinggi pada tingkat pohon adalah jenis Litsea sp. Terdapat 21 jenis pohon pakan kuskus beruang dan bagian tumbuhan yang dimakan adalah bagian daun muda dan buah. Satwa ini lebih sering memanfaatkan bagian strata tingkat C untuk melakukan aktivitasnya. Aktivitas harian yang berhasil teridentifikasi adalah aktivitas makan, berdiam, sosial, dan berpindah.
Keyword: Aktivitas harian, Karakteristik habitat, Kuskus beruang

Judul: Karakteristik Habitat dan Jenis Pakan Kuskus Beruang (Ailurops ursinus) di Suaka Margasatwa Tanjung Peropa, Sulawesi Tenggara
Abstrak: Kuskus beruang (Ailurops ursinus) yang berstatus vulnerable dalam IUCN Redlist, merupakan salah satu satwa endemik dilindungi yang hidup di hutan dataran rendah Sulawesi. Penelitian bertujuan untuk mengidentifikasi karakteristik habitat, jenis pakan, dan aktivitas harian kuskus beruang di Suaka Margasatwa Tanjung Peropa, Sulawesi Tenggara. Penelitian dilaksanakan pada bulan Februari – Maret 2017. Karakteristik habitat diidentifikasi dengan menggunakan analisis vegetasi, jenis pakan diidentifikasi dengan menggunakan pengamatan langsung dan tidak langsung, serta aktivitas harian diidentifikasi dengan menggunakan adlibitum sampling. Indeks nilai penting tertinggi tingkat pohon pada jalur Mobula dan jalur Mata Air berturut-turut yaitu Pisonia umbellifera (28.33%) dan Chisocheton ceramicus (44.72%). Terdapat 80 jenis tumbuhan yang tercatat sebagai potensi pakan kuskus beruang. Persentase tertinggi bagian tumbuhan yang dimakan oleh kuskus beruang yaitu pucuk (36.19%) dan daun muda (36.19%). Aktivitas harian kuskus beruang yaitu beristirahat (89.05%), berpindah (5.83%), mencari makan (2,71%), dan makan (2,41%). Kuskus beruang lebih sering memanfaatkan vegetasi dengan strata C dalam melakukan aktivitas hariannya.
Keyword: aktivitas harian, karakteristik habitat, kuskus beruang, pakan, sulawesi

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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