Judul: Pemodelan pemrograman dinamis pada multiple sequence alignment untuk perancangan primer Selulase
Abstrak: The role of cellulase is absolutely very important to degrade cellulose which is abundant in environment like biomass waste. Many microorganisms have capability to produce cellulase. In order to improve the cellulase production by the microorganism, one of methods can be done by inserting the sequence gene of cellulase. The information of the sequence gene can be extracted from microorganisms of cellulase producer. The first step to predict the sequence gene of cellulase, we should design the primer of the sequence gene by summarizing the information from many microorganism which are cellulase producer. The purpose of this study is the potential cellulase primers design from DNA sequence encoding cellulase alignment to be performed by multiple sequence alignment techniques using progressive methods. The procedure of this research in general divided into 3 parts, which are data preparation, multiple sequence alignment with progressive methods and primer design. The data used in the form of genes encoding cellulase retrieved from NCBI GenBank (National Center for Biotechnology Information). This study, our approach to search for potential primer to increase the productivity of cellulase enzymes. In its design, the method used multiple sequence alignment (MSA), which uses progressive alignment algorithm. In the alignment phase, from 5 obtained a cellulase coding sequences conserved region 3 (conserved regions). While the template that is used to design the primer sequences potentially Solanum lycopersicum NM-001247953 from areas that have a high similarity (conserved region). Retrieved 46 primer pairs from conserved region 3. Each of the conserved region-1 to as many as 28 pairs of primers, conserved region-2 to as many as 13 pairs of primers and the conserved region of the 3 by 5 primer pairs.
Keyword: bioinformatics, cellulase, multiple sequence alignment, primer design

Judul: Identifikasi Bakteri Selulolitik Asal Tanah Situ Gede dengan Teknik Genetika Molekuler.
Abstrak: Cellulose is a biopolymer in nature which is dominant agricultural waste. Many practices degrade cellulose into glucose molecules by using cellulase enzyme. This research aim to identify the cellulolytic bacteria and to test cellulase activities. The methods are platting isolates, halo zone testing, DNA isolation, visualization of DNA fragmen by electrophoresis, 16S rDNA gene amplification by Polymerase Chain Reaction (PCR), 16S rDNA gene divesity analysis with restriction enzymes, DNA sequensing analysis, and enzymatic assay using CMC selective media. Six isolates were analyzed by restriction enzyme and showed three diversity of based, its differences by size. The size of the bases are 1500 bp, 850 bp, and <850 bp. Sequensing DNA by GeneBank in NCBI web are 85% indentical between Cellvibrio fibrivorans to isolate D714 and then 81% identical to Cellvibrio gandavensis to isolate J531. Enzyme activities of isolates D714, D533, J731, and J531 has been tested enzyme to get the highest activity on seventh day is 0.1054 IU/mL, 0.0996 IU/mL, 0.101 IU/mL, and 0.9689 IU/mL.
Keyword: cellulolytic bacteria, cellulase enzyme, gene 16S rDNA

Judul: Faktor-Faktor yang Memengaruhi Permintaan Impor Kopi oleh Jepang.
Abstrak: Jepang merupakan negara dengan tingkat konsumsi kopi keempat tertinggi di dunia. Volume impor kopi Jepang pada tahun 2019 mencapai 470 ribu ton. Indonesia merupakan salah satu negara eksportir kopi terbesar ke Jepang, namun dalam sepuluh tahun terakhir mengalami penurunan volume ekspor. Penelitian ini bertujuan untuk menganalisis kondisi pasar kopi Jepang serta menganalisis faktor-faktor yang memengaruhi impor kopi oleh Jepang. Penelitian menggunakan data panel periode 2005-2018 dari sepuluh negara. Variabel independen yang digunakan berupa GDP riil per kapita Jepang, nilai tukar dolar AS terhadap yen Jepang, total konsumsi kopi Jepang, dan harga impor kopi oleh Jepang. Metode kuantitatif menggunakan analisis regresi, sedangkan metode kualitatif menggunakan analisis deskriptif. Mayoritas konsumen kopi di Jepang memiliki rentang umur 40-59, dengan jenis kopi yang disukai adalah kopi instan, dan dikonsumsi di rumah. Hasil analisis regresi menunjukkan bahwa nilai tukar dolar AS terhadap yen Jepang berpengaruh signifikan dan memiliki hubungan negatif terhadap permintaan impor kopi oleh Jepang.
Keyword: Coffee market, import demand, Japan, panel data