Judul: Pancreatic Lipase Inhibition and Antioxidant Activity of Kelor Leaf (Moringa oleifera) as Antiobesity in Vitro
Abstrak: Daun kelor berpotensi sebagai antiobesitas, tetapi belum diketahui metabolit yang terlibat sehingga identifikasi senyawa diperlukan. Penelitian ini bertujuan menentukan fraksi terbaik, menganalisis fitokimia, aktivitas antioksidan dan inhibisi enzim lipase, serta membandingkan korelasi antara semua perlakuan. Daun kelor disonikasi dengan etanol 70%, lalu difraksinasi bertingkat menggunakan n heksana, kloroform, etil asetat, dan akuades. Total phenolic content (TPC) dan total flavonoid content (TFC) diukur secara kolorimetri dengan Folin-ciocalteu dan alumunium klorida. Aktivitas antioksidan diukur menggunakan metode DPPH dan FRAP. Inhibisi lipase pankreas diukur secara enzimatis dengan p-nitrophenyl glucopyranoside. Hasil TPC, TFC, DPPH, dan FRAP tertinggi berturut-turut dimiliki oleh ekstrak akuades (138,88 ± 4,42 mg GAE/g ekstrak), etil asetat (113,31 ± 2,02 mg QE/g ekstrak), etanol 70% (112,49 ± 0,33 mg GAE/g ekstrak), dan akuades (1263,57 ± 0,04 mg TE/g ekstrak). Nilai IC50 paling rendah yaitu ekstrak akuades 545,87 ppm. Dengan demikian, ekstrak akuades berpotensi untuk dikembangkan sebagai obat herbal antiobesitas., Kelor leaves have potential as an anti-obesity agent, but the metabolites aren’t yet known so compound identification is necessary. This research aimed to determine the best fraction, analyze phytochemicals, antioxidant and lipase enzyme inhibition activity, and compare the correlation between all treatments. Kelor leaves were sonicated with 70% ethanol and then fractionated using n-hexane, chloroform, ethyl acetate, and distilled water. Total phenolic content (TPC) and total flavonoid content (TFC) were measured colorimetrically with Folin-ciocalteu and aluminum chloride. Antioxidant activity was measured using the DPPH and FRAP methods. Pancreatic lipase inhibition was measured enzymatically with the p-nitrophenyl glucopyranoside. The highest TPC, TFC, DPPH, and FRAP results were respectively obtained by distilled water (138.88 ± 4.42 mg GAE/g extract), ethyl acetate (113.31 ± 2.02 mg QE/g extract), ethanol 70% (112.49 ± 0.33 mg GAE/g extract), and distilled water (1263.57 ± 0.04 mg TE/g extract). The lowest IC50 value was distilled water 545.87 ppm. Thus, distilled water extract has the potential to be developed as an anti-obesity herbal medicine.
Keyword: Antioxidants, Inhibition, Kelor leaves, Pancreatic lipase, Phytochemicals

Judul: Antioxidant Capacity and Inhibition of Pancreatic Lipase  from Fractionated Asam Gelugur (Garcinia atroviridis) In vitro
Abstrak: Obesitas adalah penyakit dengan penimbunan lemak dalam jangka panjang yang mengakibatkan timbulnya penyakit degeneratif. Asam gelugur dapat bermanfaat sebagai antioksidan dan inhibitor lipase pankreas. Penelitian ini bertujuan mengukur kapasitas antioksidan, kadar fenolik dan flavonoid total, serta inhibisi lipase pankreas ekstrak kasar dan hasil fraksinasi asam gelugur. Ekstraksi dengan metode sonikasi pelarut etanol 70% kemudian difraksinasi bertingkat dengan pelarut n-heksan, etil asetat, kloroform, dan akuades. Kadar fenolik total asam gelugur tertinggi dimiliki fraksi akuades 535,80 mg GAE/g ekstrak. Kadar flavonoid total tertinggi pada fraksi kloroform 751,04 mg QE/g ekstrak. Kapasitas antioksidan tertinggi pada metode FRAP ekstrak kasar dan pada DPPH fraksi akuades dengan nilai berurut yaitu 339,82 mg TE/ g ekstrak dan 936,52 mg TE/ g ekstrak. Inhibisi lipase pankreas pada konsentrasi 1000 mg/mL tertinggi dimiliki fraksi n-heksan sebesar 91,33% dan tidak berbeda nyata dengan orlistat 88,46%. Korelasi positif diperoleh antara kapasitas antioksidan metode DPPH dengan senyawa fenolik maupun flavonoid. Inhibisi lipase pankreas dengan kadar fenolik dan flavonoid total serta kapasitas antioksidan tidak berkorelasi., Obesity is caused by long-term fatty accumulation leads to degenerative diseases. Asam gelugur can be useful as an antioxidant and pancreatic lipase inhibitor. This study aimed to measure total phenolic and flavonoid content, the antioxidant capacity, and pancreatic lipase inhibitor of crude extract and fractionated. Sonication method is used with ethanol 70% solvent and then multistage fractionation with n-hexane, ethyl acetate, chloroform, and aquades. The highest total phenolic content was observed in aquades fraction 535,80 mg GAE/g extract. The highest total flavonoid content was in the chloroform fraction 751,04 mg QE/g extract. The highest antioxidant capacity was obtained in FRAP method of crude extract and in DPPH of aquades method by aquades fraction with values of 339,82 mg TE/g extract and 936,52 mg TE/g extract. Inhibition of pancreatic lipase at 1000 mg/mL was highest in the n-hexane fraction at 91.33%. A positive correlation was found between the antioxidant capacity of DPPH method and phenolic and flavonoid contents. Pancreatic lipase inhibition with total phenolic and flavonoid and antioxidant capacity were not significantly correlated.
Keyword: fractionation, Antioxidant, pancreatic lipase inhibitor., Garcinia antroviridis

Judul: Implementation of Spatio-Temporal Change Query in Relational Database.
Abstrak: Geographic object changes in various views. Object changes are recorded using a model of static storage. No historical connection between old version data and new version data has been built. For that, a storage model of spatio-temporal queries that can analyze spatio-temporal changes has been developed. The object changes the geometry are grouped according to the taxonomy of Spatio-Temporal Pattern Change. This research has been able to apply the query patterns change including born, expand, merge, contract, split, normal and die. This query based on geometric (area) changes. The implementation of spatio-temporal change queries represents an object changes continuously with easy, change detection is done by comparing two versions of data. Keyword: spatio-temporal change query, spatio-temporal change pattern.
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