Judul: Analisis kanonik hubunga indikator kependudukan dengan derajat kesehatan
Abstrak: Bernat Simbolon. Analisis Kanonik Hubungan Indikator Kependudukan dengan Derajat Keseliaran ( di bawah bimbingan Bambang Sumantri dan Abdurrauf Rambe). Penelitian ini berujuan utrtuk menggambarkan tingkat kekuatan hubungan beberapa indikator kependudukan terhadap derajat kesehatan dan mengelompokkan propinsi di Indoriesia berdasarkan kumpulan peubah tersebut. Fakror-fakror yang mampu memberi irformasi dasar mengenai indikator kependudukan adalah kesehatan, gizi, pendidikan, ckononri, sosial budaya dun lingkungan sosial. Sedangkan derajat kesehatan secara kuantitatifdiukur dari angka kematian bayi, angka kematian balita dan harapan hidup masyarakat (laki-laki dan wanita).
Keyword: 

Judul: Pengelompokan Propinsi Di Indonesia Berdasarkan Beberapa Indikator Kesehatan
Abstrak: Pembangunan kesehatan memiliki tujuan UIltuk meningkatkan kesehatan masyarakat. Walaupun tclah banyak kemajuan yang tclah dicapai dalam bidang kesehatan, tetapi masih ada sebagian masyarakat Indonesia yang masih sulit untuk mendapatkan pelayanan kesehatan. Penelitian dilakukan wltuk lllengelolllpokkan propinsi di Indonesia berdasarkan beberapa indikator kesehatan dalam upaya mempcnTIudah mencapai Indonesia sehat 2010. Analisis gerombol dilakukan dua tahap, dengan mctode Gerombol Hirarki Pautan Rataan dan ukuran jarak Euclid. Analisis gerombol tahap pel1allla menghasilkan tiga gerombol. Gerombol II dan III cenderung lllelllencil dibanding gerombol lainn),a. Analisis tahap kedua dilakukan dengan menggerolllbolkan propinsi-propinsi pada geromboll. Pada tahap ini dihasilkan empat gerombol. Propinsi Nusatenggara Barat dan Timur, Kalimantan Barat, Maluku dan Aceh memiliki tingkat kesehatan yang relatif rendah, Ulltuk itu propinsi-propinsi tcrsebut sebaiknya memperoleh perhatian yang lebih intensif dalam bidang kesehatan. Sedangkan propinsi Jawa-Bali tingkat kesehatannya relatif tinggi.
Keyword: 

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.