Judul: Analisis Stabilitas Bendung dan Rembesan pada Perencanaan Bangunan Bendung di Desa Sungai Buntu Karawang
Abstrak: Water resources management, especially around the coast in agriculture today is needed to support empowerment in agriculture. The purpose of this study is to analyze the slope stability and seepage weir urugan as design of the weir and in Tegal River, River Buntu Village, Pedes subdistrict, Karawang . The results showed that the weir estimates are appropriate and can be built is a concrete weir with a weir height and weir urugan 1,7 m, 0,95 m high water upstream and downstream water levels 0,75 m . Shear force values and bolster the stability of the concrete weir obtained are still below the safety factor , the slice force and shear force while receiving and not receiving the load of each load is still allowed. The planned dam has a value of soil cohesion 1,8 kN/m3 and angle in the style of 34 . Safety factor for slope stability at 1,815 and seepage discharge that occurs in the soil is equal to 1,7408 x10-4 liter/second.
Keyword: seepage at urugan weir., urugan slope stability, weir concrete stability

Judul: Analisis Rembesan dan Gaya Angkat Pada Perencanaan Bendung di Desa Sungai Buntu, Kecamatan Pedes, Karawang.
Abstrak: The construction of a dam is built crossing river stream for elevating water level. In designing a dam, force field analysis in a dam is needed to ensure a dam can be operated properly. The focus of this research is to analize seepage and uplift pressure in a dam. The objective of this research is quantifying and analyzing the force that occurs in a dam as a reference in designing of a dam construction. The study is located in Sungai Buntu Village, Pedes, Karawang. The analysis is using guideline KP-02 Criteria for Designing Main Dam and KP-06 Criteria for Designing Additioal Structures. Software Geo Studio Seep/w 2004 is used in seepage analysis. The data which are required are soil permeability, and water level at upstream and downstream. The equipments used in this study are soil ring, tape, computer, and calculator. Based on the result of Geo Studio Seep/w 2004 analysis, the quantity of seepage under the Dam is 1,01 x10-8 m3/sec. Based on flow net analysis the quantity of seepage under the Dam is 1,72x10-8 m3/sec and the uplift pressure is 2450 kg/m.
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Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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