Judul: Studi Tingkat Kerusakan Pohon di Hutan Kota Srengseng Jakarta Barat
Abstrak: Ruang terbuka hijau di perkotaan kualitasnya semakin berkurang. Hal ini dapat disebabkan oleh perubahan kota dengan berkembangnya kawasan industri, perumahan, perdagangan dan lain sebagainya. Perubahan tersebut mengakibatkan ruang terbuka hijau menjadi lahan untuk peruntukan lainnya. Berkurangnya kualitas ruang terbuka hijau dapat pula disebabkan oleh adanya kerusakan dan penyakit yang diderita oleh pohon-pohon hutan kota. Pohon-pohon yang lemah lambat laun mengundurkan diri atau mati, sehingga keberadaan pohonnya akan berkurang.
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Judul: Tipe Kerusakan Pohon Hutan Kota (Studi Kasus : Hutan Kota Bentuk Jalur Hijau, Kota Bogor - Jawa Barat)
Abstrak: Nowadays the quality of environment in urban area is getting decrease. One of ways that initiated concerning urban environmental quality management is setting urban forest in appropriate type and form based on main environmental problem issues in certain urban area. Greenbelt is one of urban forest form that most developed today. The greenbelt of roadside has many functions such as providing shade, trapping and absorbing the pollutants, reducing the noise and esthetical function. Trees are the main component to set the greenbelt. As trees are the most important component of greenbelt, health monitoring and maintaining of trees are needed in urban forest management. One of the methods used for monitoring the trees’ health is by inventing the trees’ damages. It is important since trees’ damage is potential in causing accidents and material loss. The aims of this study are to identify level of trees’ damages at roadside, identify types of trees’ damages and identify tress’ damage that potential causing accident. The result from this study hopefully can be useful to providing about the trees’ damage along greenbelt of dr. Semeru roadside and a recommendation to prevent and reduction the trees damage in greenbelt of dr. Semeru street of Bogor city. This study was conducted during 3 months (September-November 2009) along dr. Semeru street. Trees’ damages inventory was using Forest Health Monitoring method by observing three indications of tress’ damages, those were location of the damages, type of damages and level of seriousness condition of the trees. The trees’ damage along greenbelt of dr. Semeru roadside achived 91.38% that equal to 371 trees out of 406 total of trees there. Trees damage level was included as low level since the Damage Index Value (DIV) showed 6.12 (the highest scale is 21). There were 709 tress’ damage types. The biggest to smallest types of trees’ damages consisted of cancer (169 cases = 23.84%), broken or dead branch (116 cases = 16.36%), conks, fruiting bodies, and other indicators of advanced decay (109 cases = 15.37%), open wound (74 cases = 10.44%), brooms on roots or bole (68 cases = 9.59%), excessive brancing or brooms (53 cases = 7.48%), damaged foliage or shoots (29 cases = 40.09%), lost of apical dominance, dead terminal (27 cases = 3.81%), resinosis or gummosis (25 cases = 3.53%), broken bole or roots lees than 0.91 m (23 cases = 3.24%), broken or dead roots beyond 0.91 m (16 cases = 2.26%). There were 22 decay and holed dangerous tress. Cavity treatments can be done to maintain those trees but if the decays and holes were not forgiven, the best treatment is trees cutting.
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Judul: Influenza Specific Immunoglobulin Y Purification from Egg Yolk with Sodium Chloride Precipitation Method
Abstrak: Imunoglobulin Yolk (IgY) yang terdapat dalam kuning telur telah banyak dimanfaatkan untuk imunoterapi dan imunodiagnostik. Salah satu kendala dalam pemurnian IgY dari kuning telur adalah pemisahan Ig dengan lemak, sehingga teknik pemurnian Ig selalu berkembang. Penelitian ini bertujuan untuk melakukan pemurnian IgY dari kuning telur dengan metode pengendapan menggunakan sodium chloride (NaCl) serta melakukan karakterisasi IgY spesifik influenza. Sodium chloride dipilih karena bahan kimia ini relatif murah, ramah lingkungan, dan tidak merusak imunoaktivitas IgY. Kuning telur yang digunakan dalam penelitian ini berasal dari telur ayam layer yang divaksinasi dengan vaksin influenza Flubio® yang berisi virus Influenza A/H3N2, A/H1N1 dan B/Washington. Pemurnian IgY hanya dilakukan pada kuning telur yang mengandung antibodi spesifik influenza. Deteksi keberadaan antibodi spesifik influenza ditentukan dengan uji agar gel presipitasi (AGP) dan metode enzyme linked immunosorbent assay (ELISA). Konsentrasi IgY hasil pemurnian dihitung dengan spektrofotometer dan karakterisasi protein dilakukan dengan metode Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Konsentrasi protein dari hasil pemurnian IgY memiliki nilai rata – rata 50,403 mg/mL. Hasil SDS-PAGE menunjukkan enam pita protein, yaitu struktur IgY utuh (174,63 kDa), Apoprotein-LDL (129,77 kDa), rantai berat IgY (66,54 kDa), Fab ( 47,65 kDa), rantai ringan IgY (21,85 kDa), dan β-livetin (36,74 kDa). IgY hasil pemurnian masih dapat berikatan dengan antigen influenza berdasarkan uji ELISA. Hasil penelitian ini menunjukkan bahwa pemurnian IgY menggunakan metode pengendapan NaCl dapat memurnikan IgY dengan struktur utuh, meskipun masih ada dua protein tidak spesifik hasil pemurnian. IgY yang dapat berikatan dengan antigen influenza mengindikasikan prospek pemanfaatannya untuk pencegahan influenza dan imunodiagnostik., Yolk immunoglobulin (IgY) contained in egg yolk has been widely used for immunotherapy and immunodiagnostics. One of the obstacles in IgY purification from egg yolk is separating Ig from fat, so that IgY purification techniques are always developing. This study aimed to purify IgY from egg yolk using sodium chloride (NaCl) precipitation method and to characterize influenza-specific IgY. Sodium chloride was chosen because this chemical is relatively unexpensive, environmentally friendly, and does not impair IgY immunoactivity. The egg yolks used in this study were collected from layer eggs vaccinated with the Flubio® influenza vaccine, which contains the Influenza A/H3N2, A/H1N1 and B/Washington viruses. IgY purification was only carried out on egg yolks containing influenza specific antibodies. The presence of influenza-specific antibodies was determined by the agar gel precipitation (AGP) test and the enzyme linked immunosorbent assay (ELISA) method. The purified IgY concentration was determined using spectrophotometer, and protein characterization was performed using the Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) method. Protein concentration from purification has a mean value of 50,403 mg/mL . SDS-PAGE result showed six protein bands, namely intact IgY structure (174,63 kDa), Apoprotein-LDL (129,77 kDa), IgY heavy chain (66,54 kDa), Fab (47,65 kDa), IgY light chain (21,85 kDa), and β-livetin (36,74 kDa). The purified IgY still able to bind with influenza antigen based on the ELISA test. The results of this study indicate that purification of IgY using the NaCl precipitation method can purify IgY with intact structure, although there are still two unspecific proteins in the purification results. The resulting IgY can bind to influenza antigens indicating the
Keyword: Immunoglobulin Y, influenza, egg yolk, purification, sodium chloride