Judul: Verifikasi Metode Analisis Serat Pangan Dengan Metode AOAC Dan Asp Terhadap Parameter Repeatability, Selektivitas, Dan Ruggedness
Abstrak: Methods of analyzing dietary fiber (DF) have undergone extensive development in the last two decades. At present, the most widely used methods are the enzymatic-gravimetric AOAC official method and the enzymatic-chemical Englyst method. Enzymatic-gravimetric was used in this research because of its economic value. New modifications of the AOAC method are continuously being proposed by researchers. We need to ensure that the method has good performance by verification method. The purpose of this study was to verified enzymatic-gravimetric method for analyzing DF. Four kind of food, raw peanut, raw soybean, carrot, and oat were analyzed for total DF, soluble DF and insoluble DF content by two current enzymatic-gravimetric methods; AOAC Official Methods (1999) and the Asp Method (1992). The results obtained by these two methods were compared. Results for raw soybean were significantly different while other samples were relatively similar. Different enzyme used in two methods leads to variance of DF content for high protein sample. Asp method could efficiently hydrolyze protein because it has two enzyme hydrolyzing step. Ruggedness test was carried out to find the best concentration of ethanol for precipitate SDF between 95% and 78%. Ethanol 78% could precipitate SDF.
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Judul: Perbandingan Metode dan Verifikasi Analisis Total Karbohidrat dengan Metode Luff-Schoorl dan Anthrone Sulfat
Abstrak: Carbohydrate plays crucial role in food industry. Therefore an accurate, direct and reliable carbohydrate analysis is needed. Among many colorimetric methods for carbohydrate determination, the Anthrone-sulfuric acid is the most commonly used. The Anthrone-sulfuric method for carbohydrate analysis is simple and sensitive. However, the SNI official method for carbohydrate analysis employing the Luff-Schoorl method which is time consuming, difficult for untrained staff and the reduction reactions are seldom stoichiometric. Therefore a new candidate method employing Anthrone sulfuric acid was proposed to replace the SNI 01-2891-1992 total carbohydrate analysis. In this research both methods were compared using three matrices which represent general food matrices in liquid form based on AOAC proposed triangle scheme. Samples from the low, medium and high content of carbohydrate from the triangle scheme were selected. The selected samples were coconut milk, soy sauce and sweet soy sauce. Based on the comparation result, Anthrone method as a new proposed method proved ineligible to replace the SNI 01-2891-1992. Thus the next step taken was to verify the SNI 01-2891-1992 method through its repeatability and accuracy. Accuracy was accessed using reference material and standard addition. The repeatability showed acceptable precision. But the standard addition exhibited poor recovery value in SNI 01-2891-1992 method of total carbohydrate
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Judul: Etno-ornitologi Burung Kancilan Flores (Pachycephala nudigula nudigula Hartert 1897) pada Masyarakat Lio di Taman Nasional Kelimutu
Abstrak: Bare-throathed whistler is one of several wild birds in Kelimutu National Park (TNKL) that is believed to have correlation with Lio Community‟s belief. The estimation of Bare-throated whistler‟s population in Kelimutu National Park was about 186.41-1668.07 individual by the density about 0.0389-0.3481 ind/ha with the sex ratio 6.2:1 that spreaded on area with height of 1100 – 1600 masl. The characteristic of Bare-throated whistler‟s habitat is a natural forest with various canopy level, high plant species diversity and high density. The habitat characteristics related to the availability of food in the form of insects, cover, safety and a nesting site. Generally the most of Lio community knew the existence of Bare-throated whistler directly or from their parents‟ stories. The perceptions of Lio Community to the Bare-throated whistler‟s existence is generally support the conservation efforts. The perceptions construct the Lio Community‟s atitude that tend to be conservative to the existence of Bare-throated whistler. The attitude shown by their behavior that not cathing the Bare-throated whistler from the TNKL‟s area or the outside. The attitude have an important role to the existence of Bare-throated whistler in Kelimutu National Park‟s area.
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