Judul: Uji kinerja promoter gen LEAFY dan AGAMOUS tanaman kakao (Theobroma cacao L.) pada tanaman tembakau (Nicotiana tabacum L.)
Abstrak: Performance of cocoa’s (Theobroma cacao L.) gene promoters, TcLFY and TcAG, in a tobacco plant (Nicotiana tabacum L.) used as a plant model has been tested. The two promoters were introduced into the tobacco plant cells using a modified leaf discs method employing Agrobacterium tumifaciens AGL0. The transformed tobacco plant cells were then regenerated and their morphology were observed periodically. The tobacco plants harbouring the gene promoter TcLFY, during regeneration, showed a leaf morphology similar to that of the positive control. However, the growth rate of tobacco plants inserted with promoter TcLFY was faster compared to that of the positive control. Tobacco plants harbouring gene promoter TcAG, during regeneration, showed imperfect leaf morphology. The growth rate of these tobacco plants was slower than that of the positive control and of the tobacco plants inserted harbouring gene promoter TcLFY. These results indicated that the introduced promoters affect the morphology of the tobacco plants. In order to further confirm the presence of the GFP gene and activation of TcLFY: GFP and TcAG: GFP, a molecular verification test was carried out. Variation of sucrose concentration in the media was made to examine the effect of sucrose on the activity promoters TcAG and TcLFY in tobacco plants. The sucrose concentration tested were 0%, 0,5% and 4% followed by observation on the 3rd and 7th day of cultivation. At 0% sucrose concentration the tobacco plants harbouring promoter TcLFY showed yellowish leaf, while those cultivated in the media with sucrose concentration of 0.5% and 4% grew well and normal. This indicated that sucrose affects the activity of the promoter TcLFY in tobacco plants. Tobacco plants inserted with promoter TcAG did not show significant morphological changes when grown in a medium of 0%, 0,5% or 4% sucrose concentration. This indicated that addition of sucrose does not significantly affect the induction of expression of the AG gene and the activation of the promoter TcAG.
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Judul: Transformasi Genetik Tanaman Tembakau oleh Gen LFY Kakao (TcLFY) Menggunakan Agrobacterium tumefaciens
Abstrak: LFY is a protein that have important role in flowering. TcLFY gene have been isolated from cacao (Theobroma cacao L.). To examine its role, this gene that is under control of p35S CaMV promoter was introduced in the tobacco plant using Agrobacterium tumefaciens. Transformation efficiency of transgenic plants was 16 % with regeneration efficiency was 27.38%, and produce 4 buds each explant. PCR analyses toward 5 putative transgenic tobacco plants that were taken randomly showed that all of them were transgenic. They also flowered early. Expression analyse of TcLFY gene using PCR showed that transgenic plant expressed the TcLFY gene in the all of its parts. This result showed that transgenic tobacco plant contained TcLFY gene under control of p35S CaMV
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Judul: Solusi Geometri Schwarzschild untuk Dimensi 4+1 dengan Invarian Rotasi Isotropik.
Abstrak: Metrik Schwarzschild merupakan solusi eksak pertama dari persamaan medan Einstein dengan mangasumsikan metrik statik dan simetris. Metrik Schwarzschild yang ditemukan oleh Karl Schwarzschild masih dalam dimensi 3+1, namun seiring dengan teori-teori terbaru yang menunjukkan bahwa alam semesta tidak hanya di dimensi 3+1. Maka melalui penambahan dimensi, tujuan penelitian ini memperoleh solusi pada geometri Schwarzschild untuk dimensi 4+1 dengan memodifikasi koordinat bola yang invarian rotasi isotropik dan melalui penurunan rumus seperti koordinat bola, simbol Christoffel, dan tensor Ricci. Selain mendapatkan solusi ini, metrik Schwarzschild dimensi 4+1 dapat digunakan sebagai metrik dasar untuk memperoleh solusi blackhole dan wormhole pada dimensi 4+1. Hasil akhirnya, dengan memanfaatkan modifikasi koordinat bola, metrik Schwarzschild dapat dibentuk sehingga diperoleh solusi geometri Schwarzschild untuk dimensi 4+1.
Keyword: metrik Schwarzschild, persamaan medan Einstein, modifikasi koordinat bola, penambahan dimensi, invarian rotasi isotropik