Judul: Analisis Manfaat Sosial Taman Kota pada Beberapa Taman di Kota Bogor
Abstrak: Ruang terbuka hijau merupakan bentuk ruang terbuka publik yang keberadaannya tidak dapat dipisahakan dari masyarakat sebagai wadah pendukung manfaat ekologi, sosial, budaya, ekonomi, dan estetika. Masyarakat kota memiliki aktivitas yang sama dan dilakukan secara berulang akan memicu terjadinya kejenuhan dan stress. Keadaan ini dapat diatasi oleh adanya taman kota kepada masyarakat melalui manfaat sosialnya seperti wadah interaksi sosial, rekreasi, pendidikan. Tujuan penelitian ini adalah mengetahui persepsi dan preferensi masyarakat, mengidentifikasi manfaat sosial, mengetahui hubungan antara usia, pendidikan terhadap persepsi masyarakat, serta menyusun rekomendasi. Penelitian ini dilaksanakan di Taman Kencana, Taman Sempur, dan Taman Heulang. Metode yang digunakan adalah nilai skala likert, nilai rata-rata, semantic differential, dan spearman correlation. Hasil analisis menunjukkan bahwa Taman Kencana memiliki nilai rata-rata manfaat sosial paling tinggi yakni sebesesar 3,30, sedangkan Taman Heulang sebesar 3,27, dan Taman Sempur sebesar 3,25. Penelitian ini menunjukkan bahwa tidak terdapat hubungan antara usia dan tingkat pendidikan terakhir dengan persepsi masyarakat. Rekomendasi yang diberikan yaitu perubahan bentuk bangku taman, penataan ruang secara bijak, dan penambahan fasilitas umum seperti papan irformasi jenis tanaman, musala, dan toilet.
Keyword: manfaat sosial, persepsi dan prferensi masyarakat, ruang terbuka hijau, taman kota

Judul: Ecological Analysis Of City Parks For Sustainability In Bogor City.
Abstrak: Taman kota sebagai salah satu jenis ruang terbuka hijau publik memiliki peran penting dalam meningkatkan kualitas lingkungan perkotaan dan mendukung keberlanjutan kota. Untuk mengetahui sejauh mana pelaksanaan fungsi ekologi dan persepsi serta preferensi masyarakat perlu dilakukan evaluasi secara menyeluruh terhadap aspek-aspek tersebut. Penelitian ini bertujuan untuk mengidentifikasi vegetasi, menganalisis kesesuaian fungsi ekologis, dan menganalisis persepsi pengguna dan preferensi terhadap lanskap di setiap taman untuk kelestarian Kota Bogor. Penelitian ini berlokasi di tiga taman di Kota Bogor, yaitu Taman Heulang, Taman Sempur, dan Alun-alun Kota Bogor. Metode penelitian ini dibagi menjadi dua metode yaitu analisis fungsi ekologis dan analisis persepsi dan preferensi pengunjung pada masing-masing taman. Fungsi ekologis akan dianalisis dengan membandingkan kondisi eksisting dengan kriteria baku fungsi ekologis, sedangkan analisis persepsi dan preferensi masyarakat mengenai ekologis taman akan dianalisis dengan pengumpulan data melalui kuesioner. Hasil evaluasi ini menunjukkan bahwa Taman Heulang merupakan taman terbaik dalam hal pengurangan kebisingan, modifikasi temperatur (teduh) dan fungsi penahan angin. Taman Sempur memiliki vegetasi yang cukup baik dalam memenuhi fungsinya sebagai pengubah suhu (teduh) dan pengatur kelembaban. Alun-alun Kota Bogor merupakan taman terbaik dalam hal fungsi pengatur kelembaban udara dan cukup baik dalam fungsi penahan angin dan pengurangan kebisingan., City parks as a type of public green open space have an important role in improving the quality of the urban environment and supporting city sustainability. To find out the extent of the implementation of ecological functions and the perceptions and preferences of the community, it is necessary to carry out a comprehensive evaluation of these aspects. This research aims to identify vegetation, analyze the suitability of ecological functions, and analyze user perceptions and preferences for the landscape in each park for the sustainability of Bogor City. This research was located in three parks in Bogor City, namely Heulang Park, Sempur Park, and Bogor City Square. This research method is divided into two methods, namely ecological function analysis and analysis of visitor perceptions and preferences in each park. Ecological function will be analyzed by comparing existing conditions with standard criteria for ecological function, while analysis of community perceptions and preferences regarding park ecology will be analyzed by collecting data through questionnaires. The results of this evaluation show that Heulang Park is the best park in terms of noise reduction, temperature modification (shade) and wind barrier function. Sempur Park has vegetation that is quite good at fulfilling its function as a temperature changer (shade) and humidity regulator. Bogor City Square is the best park in terms of air humidity control function and quite good in terms of wind barrier and noise reduction functions.
Keyword: Analysis, City Park, Ecological Function, Green Open Space, Perception and Preference

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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