Judul: Eksternalitas perladangan di sub DAS Kosinggolan Kecamatan Dumoga Kabupaten Bolaang Mongondow
Abstrak: Tujuan penelitian ini adalah untuk mengetahui berapa besarnya erosi akibat perladangan di bagian atas. Sub DAS Kosinggolan dan untuk mengetahui besarnya manfaat perladangan dibandingkan dengan eksternalitas negatif yang ditimbulkan oleh perladangan.
Keyword: Eksternalitas, perladangan

Judul: Analisis sosial ekonomi dalam pengelolaan dan kelembagaan pemamfaatan air irigasi : Studi kasus di Kec. Dumoga, Kab. Bolaang Mongundow, Propinsi Sulawesi Utara
Abstrak: Aspek pengelolaan dan kelembagaan irigasi sangat penting artinya bagi pemerintah khususnya, karena mahalnya investasi yang telah dicurahkan untuk membangun, mengope- rasikan dan memelihara proyek-proyek irigasi. Di tingkat tersier aspek ini lebih menjadi masalah karena melibatkan para petani selaku pemakai air. Oleh karena itu penye- lenggaraannya perlu terus dievaluasi dengan terus mengem- bangkan sistem alokasi yang mantap, memperhatikan efek- tivitas, efisiensi serta pemerataannya. Tujuan Praktek Lapang ini adalah untuk mempelajari pengelolaan dan kelembagaan irigasi di tingkat tersier, mengidentifikasi dan merumuskan permasalahan yang terjadi, dan menilai seberapa jauh kemajuan sistem irigasi yang terdapat di daerah penelitian. Dengan tipe observatif deskriptif, penelitian dilaku- kan di Kecamatan Dumoga dan data dikumpulkan dengan metoda. wawancara dan pengamatan di lapangan, di samping memanfa- atkan data sekunder yang tersedia. Hasil penelitian ini menunjukkan bahwa kelembagaan dan pengelolaan irigasi di tingkat tersier dipengaruhi ...
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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