Judul: Analisis hubungan pengetahuan gizi,motivasi, persepsi dan sikap dengan kebiasaan makan sayuran ibu rumah tangga perkotaan dan pedesaan di Bogor
Abstrak: Penelitian ini secara umum bertujuan untuk mengetahui hubungan antara pengetahuan gizi, motivasi, persepsi dan sikap dengan kebiasaan makan sayuran ibu rumah tanggga perkotaan dan pedesaan di wilayah Bogor. Tujuan khusus dari penelitian ini adalah 1) mengetahui karakteristik demografi keluarga dan ibu rumah tangga di perkotaan dan perdesaan; 2) mengetahui tingkat pengetahuan gizi, motivasi, persepsi dan sikap ibu rumah tangga di perkotaan dan perdesaan; 3) mengetahui keragaan kebiasaan makan sayuran ibu rumah tangga yang meliputi jenis dan frekuensi makan serta 4) mengetahui faktor-faktor yang berhubungan dengan pengetahuan gizi, motivasi, persepsi, sikap dan kebiasaan makan sayuran ibu rumah tangga di perkotaan dan perdesaan.
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Judul: Analisis hubungan pola asuh makan, pengetahuan gizi, persepsi, dan kebiasaan makan sayuran ibu rumah tangga di perkotaan dan pedesaan Bogor
Abstrak: Penelitian ini bertujuan untuk menganalisis hubungan pola asuh makan, pengetahuan gizi, persepsi dan kebiasaan makan sayuran ibu rumah tangga perkotaan dan pedesaan di wilayah Bogor. Secara khusus bertujuan untuk: 1) mengetahui karakteristik keluarga dan karakteristik contoh (ibu rumahtangga) di perkotaan dan pedesaan, 2) mengetahui pola asuh makan, pengetahuan gizi, persepsi, dan kebiasaan makan contoh, 3) mengetahui hubungan antara pendidikan dengan pengetahuan gizi dan persepsi contoh, 4) mengetahui hubungan antara pola asuh makan dengan pengetahuan gizi dengan persepsi contoh. 5) mengetahui hubungan persepsi dengan kebiasaan makan sayuran.
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Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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