Judul: Struktur dan Keragaman Genetik Populasi Hama Boktor (Xystrocera festiva) pada Hutan Rakyat Sengon (Falcataria moluccana) di Jawa.
Abstrak: Boktor (Xystrocera festiva) is the most destructive sengon pests, because the larvae feeding activity is done by broaching the woody tissue inside sengon trees. The objective of this research was to study population structure and genetic diversity of boktor pests in Java using RAPD markers. Boktor larvae were sampled from 10 susceptible trees in eight districts in Jawa, and then census was done to calculate the total individuals found in each tree, before taking measurements of body length and diameter of its head. Having been census a number of 10 larvae per tree of various sizes were picked up as sample for DNA analysis. DNA extraction was done using CTAB (Cetyl Trimethil Ammonium Bromide) method, and the PCR (Polymerase Chain Reaction) was done using OPP 9, OPP 15, and OPP 19 primers to get RAPD (Random Amplified Polymophic DNA) fragment. Scored fragment data was analyzed using software POPGENE 1.31 version 32 and NTSYS version 2.0. The results showed the heterogeneity of the population structure according to the diameter and body length of each population. Result of RAPD analysis showed that the highest heterozigosity (He) value was from Tasikmalaya (0.2525) and the lowest heterozigosity (He) was 0.1039 from Magelang. Dendrogram based on genetic distance showed that Kediri population was closely related with Temanggung while Ciamis population is farthest.
Keyword: CTAB, RAPD, sengon, population structure, Xystrocera festiva

Judul: Keragaman Genetik Sengon (Falcataria moluccana Miq.) Resisten dan Rentan Terhadap Hama Boktor Berdasarkan Penanda Mikrosatelit.
Abstrak: Sengon (Falcataria moluccana Miq.) merupakan jenis tanaman cepat tumbuh yang multiguna, banyak ditanam pada hutan rakyat. Pola tanam monokultur memiliki risiko terserang hama penggerek batang seperti boktor (Xystrocera festiva). Salah satu cara untuk mengatasinya adalah dengan menanam pohon resisten yang dihasilkan dari program pemuliaan. Penelitian ini bertujuan untuk mengukur keragaman genetik sengon resisten dan yang terserng hama boktor berdasarkan penanda mikrosatelit. DNA diekstraksi menggunakan Dneasy plant mini kit dari QIAGEN, kemudian diamplifikasi menggunakan lima pasang primer mikrosatelit yaitu Pafa05, Pafa06, Pafa07, Pafa08 dan Pafa10. Hasil analisis menunjukkan bahwa hanya empat pasang primer yang dapat mengamplifikasi dengan baik yaitu primer Pafa06, Pafa07, Pafa08 dan Pafa10. Populasi sengon resisten memiliki tingkat heterozigositas tertinggi (He = 0.650), dibandingkan populasi sengon yang rentan boktor (He = 0.623). Populasi sengon resisten dan rentan terhadap hama boktor memiliki jarak genetik yang cukup dekat (0.160). Keragaman genetik kedua populasi tersebar sebanyak 94.09% di dalam populasi dan 5.91% di antara populasi.
Keyword: keragaman genetik, mikrosatelit, F. moluccana, rentan, resisten

Judul: Hydroxyapatite-Chitosan Composite Coating on Stainless Steel 316 to Improve Corrosion Resistance
Abstrak: Metals that were used in bone implantation should have good corrosion resistance that were not easily corroded when implanted into the body. Metal corrosion resistance of stainless steel 316 can be enhanced through the metal coating by hydroxyapatite-chitosan composite with composition ratio of hydroxyapatite (gram) and chitosan 3% (mL) 0.2:0, 0.2:0.1, 0.2:0.5, 0.2:1.0, and 0.2:1.5. Besides improving the corrosion resistance of metal, the presence of chitosan also maintain the position of hydroxyapatite on metal surfaces. Corrosion resistance increases with increasing amount of chitosan added and the best composition of hydroxyapatite-chitosan composite was 0.2:1.0.
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