Judul: Analisis ekspresi gen penyandi enzim antioksidan pada kulit batang hevea
Abstrak: Tingginya permintaan dunia akan karet perlu diimbangi dengan usaha untuk meningkatkan produksi lateks di antaranya melalui penggunaan stimulan seperti etefon. Stimulasi etefon yang berlebiian oleh tanaman kmt difespons sebagai cekaman bagi kehidupannya sehingga menyebabkan akumulaqi Reactive Oxygen Species (ROS) yang memicu kematian sel. Penelitian ini bertujuan mempelajari ekspresi gen penyandi enzim antioksidan yang terlibat dalam ROS-Scavenging system, yang meliputi mangan superoksida dismutase (MnSOD), askorbat peroksidase (APX), Zn-Cu superoksida dismutase (Zn-CuSOD), glioksilatkarboligase kloroplastik (GCLchl), katalase (CAT), dan glikosilatkarboligase sitosolik (GCLcyt) terhadap pengamh pelukaan dan pemberian etilen menggunakan aktin sebagai kontrol. RNA karet dari kulit batang diisolasi kemudian dikarakterisasi menggunakan spektrofotometer dan elektroforesis gel agarosa. Ekspresi gen diamati menggunakan rnetode RT-PCR.Ekspresi gen CuZnsod, gclsitosolik, Mnsod, gclkloroplastik, cat, dan Hbapx umumnya meningkat setelah dilakukan penyadapan sebanyak tujuh kali. Peningkatan ekspresi gen tertinggi diperlihatkan oleh gen Mnsod sebesar 13,38. Ekspresi gen menurut setelah diberi perlakuan penyadapan 4 hari sekali dan pemberian etilen 12 kali dalam setahun yang menunjukkan bahwa tanaman tersebut mengalami cekaman oksidatif karena kehadim ROS. Hal ini memberi peluang sebagai penanda molekuler dalam seleksi klon-klon karet berdaya hasil tinggi dan responsif terhadap aplikasi etefon.
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Judul: Expression Gene of ACC Oksidase effect of Etilena Eksogen and Tapping at Hevea brasiliensis Clone of PB 260.
Abstrak: High request of natural rubber on the world have made researcher to do increase natural rubber product and also look for or yield good clones holding to disease and have high production. This research used clone PB 260 coming from result of cross of primary clone and secondary clone. This clone have fastly of high latex metabolism but less responsive to stimulan. Effort to increase natural rubber product with treatment of tapping and usage of etephon stimulan. Etephon is compound 2-chloro-ethylposphonic acid (CEPA) as incentive or stimulant to increase produce hormone of ethilene endogen at rubber crop. Gene of HbACO1 will be test its expression of etefon and influence of tapping at bark and latex of rubber crop. This research aim to study gene expression of HbACO1 to influence of tapping and giving stimulant at various phase growth of crop. Result of research show gene of HbACO1 only have expression at bark of rubber crop not in latex. Treatment which good to bark of rubber crop is 2 day once tapping and 3 times in one year giving stimulant.
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Judul: Implementation of Spatio-Temporal Change Query in Relational Database.
Abstrak: Geographic object changes in various views. Object changes are recorded using a model of static storage. No historical connection between old version data and new version data has been built. For that, a storage model of spatio-temporal queries that can analyze spatio-temporal changes has been developed. The object changes the geometry are grouped according to the taxonomy of Spatio-Temporal Pattern Change. This research has been able to apply the query patterns change including born, expand, merge, contract, split, normal and die. This query based on geometric (area) changes. The implementation of spatio-temporal change queries represents an object changes continuously with easy, change detection is done by comparing two versions of data. Keyword: spatio-temporal change query, spatio-temporal change pattern.
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