Judul: Introduksi Gen Aroma (badh2 Termutasi) dari Varietas Pandan Wangi atau Mentik Wangi ke Ciherang
Abstrak: Aroma wangi merupakan karakter potensial pada padi kualitas tinggi yang permintaannya semakin meningkat. Permintaan tersebut sangat tinggi di pasaran Asia dan di seluruh dunia. Perbaikan varietas aromatik baru dengan karakter agronomi sebaik varietas nonaromatik merupakan langkah yang prospektif untuk meningkatkan produksi dan pertanian di Indonesia. Penelitian ini merupakan bagian dari usaha pengembangan varietas aromatik baru nontransgenik dengan mengintroduksikan gen aroma padi (badh2 termutasi) varietas aromatik (Pandan Wangi atau Mentik Wangi) ke varietas nonaromatik (Ciherang). Metode persilangan terarah (site-directed crossing) digunakan untuk menyilangkan tanaman hasil penelitian sebelumnya, yaitu BC3F1 Ciherang/Pandan Wangi (CP) dan BC2F1 Ciherang/Mentik Wangi (CM) untuk menghasilkan tanaman BC4F1 CP dan BC3F1 CM. Seleksi dilakukan menggunakan PCR dengan marka RM223 untuk CP dan marka Bradbury untuk CM. Sampel DNA padi hasil isolasi dari daun muda diamplifikasi dengan PCR dan produknya dielektroforesis agarosa 3% untuk CP dan 1.5% untuk CM. Hasil amplifikasi DNA tanaman padi BC3F1 CP menggunakan marka RM223 dapat membedakan pola pita padi aromatik dan nonaromatik dengan ukuran pita sebesar 140 bp dan 160 bp. Hasil amplifikasi DNA tanaman padi BC2F1 CM menggunakan marka Bradbury menghasilkan pita berukuran 580 bp, 355 bp, dan 257 bp. Pita heterozigot yang terbentuk menunjukkan keberhasilan introgresi gen aroma (heterozigot badh2) dari Pandan Wangi atau Mentik Wangi ke Ciherang.
Keyword: Fragrance, high-quality rice, new aromatic varieties, RM223 marker

Judul: Identifikasi Gen Aroma Pada BC5F1 Ciherang-Pandan Wangi dan BC4F1 Ciherang-Mentik Wangi
Abstrak: The demand of high quality rice becomes increasingly in Asian market and around the world. The increase in productivity and quality of Indonesian agriculture has continued, such as improving new aromatic rice variety to have good characteristics (resistant to pest, ) which is like non-aromatic rice. The aim of this research was to develop new varieties of non transgenic aromatic rice by induction of aroma gene (mutated badh2) from aromatic varieties (Pandan Wangi or Mentik Wangi) to non aromatic varieties rice (Ciherang). Rice BC5F1 CP (Ciherang/Pandan Wangi) and BC4F1 CM (Ciherang/Mentik Wangi) that used in this research were the result from previous research with site-directed crossing method. Selection of plant was done by PCR with RM223 marker for CP and Bradbury marker for CM. Visualization of DNA sample by PCR was done by using gel agarose 3% for CP and 2% for CM. DNA amplification result of BC5F1 CP with RM223 marker can distinguish the band pattern of aromatic and non aromatic with band size 140 bp and 160 bp. In addition, DNA amplification result of BC4F1 CM with Bradbury marker produced DNA band size 580 bp, 355 bp and 257 bp. The succeed in introgression of aroma gene from Pandan Wangi or Mentik Wangi to Ciherang was showed by heterozygote band.
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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