Judul: Pola Zonasi Habitat Mangrove dan Asosiasi Makrozoobentos di Wilayah Pantai Indah Kapuk, Jakarta
Abstrak: Ekosistem mangrove Pantai Indah Kapuk mengalami tekanan lingkungan yang dapat mempengaruhi zonasi mangrove serta makrozoobentos. Penelitian ini dilakukan untuk mendeskripsikan zonasi mangrove dan makrozoobentos yang berasosiasi di Pantai Indah Kapuk, Jakarta. Pengambilan data vegetasi mangrove dilakukan dengan metode kuadrat dan pengambilan contoh makrozoobentos dengan corer. Mangrove asosiasi dan Avicennia marina ditemukan di daerah dekat laut serta daerah dekat darat, sedangkan mangrove campuran ditemukan di daerah tengah. Asosiasi makrozoobentos didominasi oleh kelas Oligochaeta, Polychaeta, dan Gastropoda yang melimpah di daerah tengah. Zonasi mangrove tersebut berkorelasi dengan beberapa kelas makrozoobentos, terutama Gastropoda dan Crustacea. Pengelolaan habitat dapat dilakukan dengan pengelolaan sampah, dan rehabilitasi habitat dengan penanaman Rhizophora.
Keyword: asosiasi, mangrove, makrozoobentos, Pantai Indah Kapuk, zonasi

Judul: Distribusi Spasial Sampah Laut Di Ekosistem Mangrove Pantai Indah Kapuk Jakarta
Abstrak: Penelitian mengenai sampah laut (makrodebris dan mikroplastik) telah dilakukan di ekosistem mangrove Pantai Indah Kapuk pada bulan Maret-Juni 2014. Tujuan penelitian ini adalah untuk menganalisis potensi dan permasalahan sampah laut serta menentukan strategi pengelolaan yang tepat. Pengumpulan sampel makrodebris dilakukan dengan menggunakan transek kuadrat. Plastik merupakan tipe makrodebris dominan, tercatat 77.7% dari total makrodebris adalah plastik, diikuti oleh styrofoam (18.1%). Kelimpahan makrodebris berkorelasi positif dengan kerapatan Avicennia marina, meskipun tidak berbeda nyata antarjarak dari batas mangrove terluar. Kelimpahan mikroplastik ditentukan dengan pemisahan densitas sedimen. Sedimen mangrove mampu merangkap mikroplastik hingga kedalaman 30 cm tanpa adanya perubahan kelimpahan. Film merupakan tipe mikroplastik dominan (74.1%), diikuti oleh fiber (16.8%). Kelimpahan makroplastik tidak berkorelasi dengan kelimpahan mikroplastik. Strategi pengelolaan yang disarankan adalah dengan rehabilitasi kawasan mangrove melalui penanaman Rhizophora sp. pada batas mangrove terluar dan memperluas ketebalan ekosistem mangrove.
Keyword: mangrove, mikroplastik, Pantai Indah Kapuk, sampah laut

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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