Judul: Penentuan pH dan suhu optimum aktivitas kitinase Bacillus cereus I.5 dan pengujian kitinase dalam mendegradasi eksoskeleton kutu bertepung putih (Ferrisia virgata Cockerell)
Abstrak: Bacillus cereus I.5 is one of chitinolytic bacteria isolated from chilli rhizosphere. The chitinase could be apply as a biocontrol of plant pest. One method to precipitate enzyme by using ammonium sulphate. It could increase specific activity enzyme. This research was conducted to determine optimum pH and temperature of chitinase activity of Bacillus cereus I.5 and tested its ability to degrade chitin exoskeleton of Ferrisia virgata Cockerell, one of Jatropha’s pest. Enzyme activity was obtained at the 10% ammonium sulphate saturation level, which is 20 times higher compared to crude enzyme. Specific enzyme activity increased from 0,039 U/mg of crude extract to 0,778 U/mg after ammonium sulphate precipitation. Chitinase of B. cereus i.5 showed highest activity at pH 7,0 and 55 °C although the enzyme also showed its activity at pH 5,0-9,0 and temperature 26-≤55 oC. Application of crude chitinase and the enzyme after ammonium sulphate precipitation on degrading of chitin Ferrisia virgata showed it worked better if incubated firstly at its optimum temperature.
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Judul: Isolasi, Pemurnian Parsial, dan Karakterisasi Enzim Kitinase dari Bacillus sp. BPPT CC 2
Abstrak: Chitinase is a potential enzyme due to its acitvity hydrolyting chitin to its derivates. Therefore, it can be used for various applications, such as agriculture, microbiology, and cosmetic. This study aimed to isolate, purify, and characterize (pH, temperature, metal ion effect, molecular weight, and kinetic parameters) chitinase of Bacillus sp. BPPT CC 2. Ammonium sulphate precipitation and dialysis were used for enzyme purification. Enzyme was characaterized its optimum pH and temperature, metal ions, molecular weight, as well as kinetic parameters. The results showed that enzyme was well precipitated using 80% ammonium sulphate. After dialyzed, level of enzyme purity increased 6.58 fold with specific activity of 0.0924 U/mg. Results showed that the optimum temperature and pH of chitinase were found at 55ºC and pH 5. Value of Km and Vmaks values were 2.705x10-4mg/L second and 0.6959 mg/L. Predicted molecular weight of chitinase was 103 kDa. Addition of metal ions Mn2+and EDTA (5 mM and 10 mM) act as activators of chitinase
Keyword: Bacillus sp. BPPT CC 2, characterize, chitinase, kinetic parameter, purification

Judul: Visualisasi Data Spasial di MonetDB Menggunakan OpenJUMP
Abstrak: There are currently the most used data model is relational. This model a database as a set of data that is stored in structural rows and columns. Besides the popullarity, the relational data model is lacking in the implementation of data is large and has a high complexity. Different of the relational model, MonetDB using a column store method to divide the data into sections binary tables . This method is only necessary to call the data, resulting method is faster. Anggi ( 2013 ) have tested the MonetDB as a spatial and non spatial data storage, but not yet visualize data from MonetDB. This result successfully visualize spatial data using a simple query “like” and “and” the average time 1 second , while the complex spatial queries using “joins”, “constains” and “Intersect” with average time more than 20 minutes. So it looks that MonetDB also can process spatial data simple and complex . But in the process of complex spatial data visualization that require run-time is relatively long .
Keyword: visualize, OpenJUMP, MonetDB, spatial data