Judul: Uji Toksisitas Recombinant Growth Hormone terhadap Artemia salina Leach.
Abstrak: Hormon pertumbuhan rekombinan (rGH) merupakan protein yang diproduksi dengan memanfaatkan bakteri Escherichia coli yang membawa vektor ekspresi gen hormon pertumbuhan dan terbukti mampu meningkatkan laju pertumbuhan ikan maupun udang. Potensi toksisitas rGH ikan kerapu kertang (rElGH) belum diketahui. Penelitian ini bertujuan untuk menentukan tingkat dan golongan toksisitas rGH ikan kerapu kertang terhadap A. salina menggunakan metode brine shrimp lethality test (BSLT). Konsentrasi rElGH yang digunakan, yaitu: A. 1,5; B. 15; C. 150; dan D. 1,500 mg L-1 dengan lima kali ulangan. Sebagai kontrol, digunakan dua perlakuan, yakni perlakuan K1 hanya menggunakan 20 mL air laut, dan perlakuan K2 dengan pemberian 3 mL PBS (pelarut rElGH) dan 17 mL air laut. Kepadatan A. salina yang digunakan yakni 100 ekor dalam 20 mL larutan yang berisi 3 mL rElGH dan 17 mL air laut. Pengamatan terhadap jumlah naupli yang mati dilakukan setiap 6 jam sekali selama 24 jam pengamatan. Persentase kematian pada perlakuan A, B, C, dan D berturut-turut ialah 4,0%; 11,2%; 26,6%; dan 100%. Berdasarkan hasil analisis probit Minitab 16, nilai LC50 adalah 222,05 mg L-1, sehingga rElGH digolongkan dalam senyawa dengan tingkat toksisitas rendah.
Keyword: A. salina, BSLT, rElGH, toksisitas

Judul: Penentuan Dosis Hormon Pertumbuhan Rekombinan Ikan Kerapu Kertang Pada Larva Ikan Lele Sangkuriang Melalui Perendaman
Abstrak: Growth and survival rate are the main factor determining the productivity of aquaculture. This research was conducted to obtain the dose of recombinant giant grouper growth hormone (rElGH) that generates highest growth and survival rate of Sangkuriang catfish at larval stage through immersion. The research used a completely randomized design and consisted of four treatments with three replications. The dose of rElGH used was 0 (control), 0.2 mg/L, 2.0 mg/L and 20.0 mg/L. rElGH was dissolved in 100 mL water containing 9 g/L salt solution and 100 mg/L bovine serum albumin. A total of 100 larvae at 5 days after hatching were hiperosmotic treated on 35 g/L salt solution for 2 minutes, and then immersed into 100 mL of rElGH solution for 1 hour. Fish were reared for 21 days and fed on blood worm ad libitum. The results showed that biomass and survival rate of 2 mg/L rElGH treated fish was 49.60% and 47.67% higher than control, respectively. Thus, the biomass of Sangkuriang catfish seed can be improved by immersing in water containing 2 mg/L rElGH, and it is potentially high to be used in order to increase aquaculture production.
Keyword: immersion., recombinant growth hormone, larval catfish

Judul: Elektrodeposisi Film Tipis Semikonduktor Cu2ZnSnS4 (CZTS) pada Substrat Kaca ITO
Abstrak: Semiconductor Cu2ZnSnS4 (CZTS) thin film that can be used as absorber layers on solar cell module was made via electrodeposition method. CZTS thin film was electrodeposited on the surface of a silicate glass coated with a thin layer of indium tin oxide (ITO). Deposition process was done using potentiostatic mode at room temperature with a potential of 1.05 V for 30, 45, and 60 minutes, followed with annealing the film at 2 different temperatures, 500 ºC using furnace and 180 ºC using hot plate, both treatments for 45 minutes in N2/H2S atmosphere. The X-ray difractograms were analyzed using Match 2 program and showed that the CZTS thin films had kesterit crystalline structure, with a low percentage of CZTS phase. The thin film also contained secondary phases such as Cu2S, ZnS, and SnS. The obtained CZTS thin films had a thickness between 1.039 and 1.676 μm. Absorption study showed that the band gap energy of CZTS thin films which was annealed using furnace ranged from 1.50 to 1.52 eV, whereas that annealed using the hot plate was not observed. The band gap energy value showed that CZTS thin films can be used as sunlight absorber layers on solar cell. The formation of CZTS crystal film on this investigation was effectively performed using furnace annealing process at a temperature of 500 ºC with deposition time of 45 minutes.
Keyword: Thin film, Electrodeposition, Cu2ZnSnS4