Judul: Analysis of City Parks Ecological Function for Central Jakarta Sustainability
Abstrak: Taman Kota sebagai ruang terbuka hijau, memiliki fungsi ekologis yang berperan dalam mendukung keberlanjutan kota. Jakarta Pusat memiliki beberapa taman kota yang berpotensi mendukung keberlanjutan kota jika berfungsi dengan optimal. Penelitian ini bertujuan menganalisis fungsi ekologis dan persepsi masyarakat terhadap fungsi ekologis di Taman Menteng, Taman Situ Lembang, dan Taman Suropati. Fungsi ekologis dinilai dengan membandingkan kondisi eksisting dengan kriteria yang telah ditetapkan, dan persepsi masyarakat didapatkan melalui kuesioner. Hasil penelitian menunjukkan ketiga taman memiliki fungsi yang berjalan cukup baik, namun masih perlu peningkatan dalam beberapa fungsi. Mayoritas responden menganggap fungsi sudah berjalan baik, namun mengharapkan beberapa fungsi dapat ditingkatkan., City parks, as green open spaces, serve an ecological function that plays a crucial role in supporting urban sustainability. In Central Jakarta, a few city parks have the potential to contribute significantly to the city's sustainability if their ecological functions are optimized. This research aims to analyze the ecological functions and public perceptions regarding these functions in Taman Menteng, Taman Situ Lembang, and Taman Suropati. Ecological functions assessed by comparing the current condition of each tree against established criteria, and public perceptions gathered through questionnaire data. The results indicate that each park is functioning adequately, but there is room for improvement in certain ecological functions. The majority of respondents acknowledge that the functions are performing well; however, they express a desire for some aspects of the functions to be improved.
Keyword: City Park, Ecological Function, Green Open Space, City Sustainability

Judul: Ecological Functions Analysis of City Park for the Sustainability of South Jakarta City
Abstrak: Ruang terbuka hijau merupakan ruang hijau yang mempunyai fungsi terhadap lingkungan disekitarnya, khususnya dalam aspek ekologi. Salah satu jenis ruang terbuka hijau yang umum dijumpai di kota adalah taman kota. Penelitian ini bertujuan menganalisis kesesuaian fungsi ekologis taman dan persepsi masyarakat terhadap fungsi ekologis di Taman Mataram, Taman Puring, dan Tebet Eco Park. Metode penelitian terdiri dari dua metode analisis yaitu, analisis fungsi ekologis taman yang dilakukan dengan penilaian vegetasi pohon dan analisis persepsi masyarakat terhadap fungsi ekologis yang dilakukan dengan menggunakan kuesioner di ketiga taman tersebut. Taman Mataram dan Tebet Eco Park unggul dalam fungsi modifikasi suhu, dan ketiga taman unggul dalam fungsi penyerap polusi. Masyarakat memahami bahwa taman dapat berpengaruh dalam fungsi ekoogis lingkungan sekitarnya.
Keyword: City Park, Green Open Space, Ecological Function, Urban Sustainability

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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