Judul: Analisis kemauan membayar masyarakat perkotaan untuk jasa perbaikan lingkungan, lahan dan air (suatu kasus DAS Citarum Hulu)
Abstrak: Mengingat krusialnya peran kawasan daerah hulu DAS, termasuk DAS Citarum, dalam mengkonservasi air, maka upaya perbaikan lingkungan kawasan itu menjadi suatu keharusan. Tujuan penelitian ini adalah menganalisis persepsi dan kemauan membayar masyarakat (WTP) untuk jasa perbaikan lingkungan, menganalisis faktor-faktor yang mempengaruhi WTP tersebut, serta menganalisis pilihan sumber air masyarakat. Metode analisis yang digunakan adalah analisis deskriptif, Contingent Valuation Method (CVM), dan regresi. Hasil analisis deskriptif menunjukkan bahwa ketersediaan air makin memburuk, sehingga perlu adanya upaya perbaikan lingkungan untuk memperbaiki kondisi air, namun WTP saat ini relatif masih rendah.. Faktor-faktor yang mempengaruhi WTP untuk jasa perbaikan lingkungan adalah pendapatan, umur, tanggungan keluarga, ketersediaan air, keluhan air, dan status rumah. Sebagian besar Masyarakat masih cenderung menggunakan air sumur daripada air PDAM, atau kedua-duanya, dengan dengan berbagai alasan dan kekhawatiran.
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Judul: Penifaian Jasa Lingkungan Air Minum dan Penentuen Prioriias Bantuan Perbaikan Lingkungan (Studi Kasus DAS Citarum, Jawa Barat)
Abstrak: Willingness of community at Citarum watershed to partidpate in improving environmental quality as an effort to reduce impact of dimate change was assessed based on their willingness to pay to environmentd sewices provided by upper watershed communities. Identification of activities that would reduce the involvement of upstream communities to do deforestation and assessment, of vulnerability of downstream communities to climate change was carried out through survey to. household. The illingness to pay was analyzed using Contingent Valuation Methd (CVM) and priority pwmm was analyzed using Analytical Hierarki Process (AHP). Based on CVM analysis it was indicated that the level of willingness of downstream communities to pay the compensation for the environmental sewices (0.g. drinking water) provided by upstream communities depended on their perception and understanding about the important of maintaining forest cover at the upper Citafum watershed and availability of drinking water. If the downstream communities living in the uhan areas often exposed to clean water scarcity problem during dry-season, their willingness to pay for the compensation tended to inmase. Further analysis found that the main factors that motivate mmuniiy to do deforestation at Upper Cirum watershed were to seek new land for agricutture activities and to increase their income. Majority of communities wanted to stop destructing the forest if they have alternative jobs for income generation.
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Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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