Judul: Pencarian Varian Gen Miostatin Pada Domba Komposit Sumatera
Abstrak: Domba komposit sumatera merupakan bentuk silangan domba lokal ekor tipis Sumatera (DETS) dengan domba St. Croix (SC) dan Barbados Blackbelly (BB) dengan komposisi 50% DETS: 25% SC: 25%BB (Subandriyo et al. 2000). Miostatin atau growth differentiations factor 8 (GDF 8) merupakan anggota dari superfamili transforming growth factor-â (TGF-â) berfungsi sebagai pengontrol negatif pertumbuhan otot (McPherron et al.1997). Mutasi gen miostatin dapat mengganggu ekspresi dan aktifitasnya sehingga otot kerangka akan mengalami hipertropi ataupun hiperplasia (pertumbuhan otot yang melebihi normal), hal ini dikenal dengan fenomena double muscling. Mutasi pada makhluk hidup dapat terjadi secara alami dalam jangka waktu yang panjang, sehingga menghasilkan varian-varian baru dari makhluk hidup tersebut. Hasil pencarian varian gen miostatin pada domba komposit sumatera menggunakan dengan metode PCR-RFLP dengan enzim restriksi Hae III dan Mbo I tidak menunjukkan adanya keragaman. Metode PCR-SSCP menunjukkan adanya varian gen miostatin ekson 3 pada domba komposit dalam penelitian ini yaitu W, X, Y, dan Z dengan frekuensi berturut-turut 3.62 %, 37.35 %, 37.35 %, dan 21.69 %. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa tipe SSCP gen miostatin ekson 3 tidak nyata mempengaruhi tipe kelahiran, bobot lahir, bobot sapih, dan pertambahan bobot badan pada domba komposit sumatera.
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Judul: Identifikasi Keragaman Gen Miostatin pada Domba Lokal
Abstrak: Myostatin is a member of tranforming grown factor B superfamily, know as growth and differentiation factor, which is essensial for proper regulation of skeletal muscle mass in wide range vertebrate species. To pursue potential agricultural application of increasing muscle mass by inhibition of myostatin activities, the aim of this study was to identify the variants of myostatin gene in the Indonesian local sheeps. A total of 151 DNA samples belonging to eight different areas that are Bogor Ciomas (20), Bogor Jonggol (35), Margawati (18), Indramayu (30), Dinggala (24), MAdura (8), Sumbawa (8), and Rote (8) were analyzed with Polymerase Chain Reaction-Single Starnd Conformation Polymorphism. The Myostatin (GDF 8) amplified product was 688 bp(base pair)
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Judul: Hamiltonian formulation for linear waves motion in two layers fluid
Abstrak: The surface wave could be considered as a wave that separates two fluids, namely water and air. Based on this assumption, it is introduced the interfacial wave, a wave between two layers of fluid with different density. The formulation of interfacial waves motion begins with deriving the base equation of irrotational ideal fluid. Furthermore, according to irrotational fluid assumption, the base equation can be stated in velocity potential. In this derivation, the fluids domain is assumed to be restricted by rigid lid boundary conditions, both at the upper and lower limit. Therefore, the interfacial waves motion can be explained in a hamiltonian formulation. In the hamiltonian formulation, total energy is defined as the sum of kinetic and potential energy. The hamiltonian system is obtained from reduction of kinetic energy by using the Dirichlet Neumann Operator. The resulted kinetic energy equation is nonlinear. Therefore, this form is linearized by first part of the Taylor expansion. This linearization gives a dispersion relation of linear wave. Based on this dispersion relation, the phase speed of the linear wave depends on the density ratio of the two layers fluid.
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