Judul: Nilai gizi daging, bobot potong dan bobot karkas domba jantan pada komposisi rumput gajah dan konsentrat yang berbeda
Abstrak: Sheep farmers usually set the amount of feed given to their sheep based on the amount of dry matter required by the sheep. Practically, it is assumed, on the average, about 5 % of sheep body weight. On the field, sheep feed was cornpod of grass and concentrate. Different ratio of gras-concentrate might be able to supply the same amount of dry matter, however, might supply different nutritional composition Consequently, different gmwxncentrate ratio, which has certain nutritional supply, results in different meat nutritional value.
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Judul: Pertumbuhan, Komposisi Tubuh, Produksi Karkas dan Kualitas Daging Domba Garut dengan Manajemen Pemberian Pakan Berbeda
Abstrak: Pemanfaatan limbah tauge dapat dijadikan sebagai pakan alternatif pengganti sumber serat. Pemberian pakan sore hari dengan suhu yang lebih nyaman diduga dapat meningkatkan produktivitas ternak. Penelitian ini bertujuan untuk membandingkan pengaruh jenis pakan dan waktu pemberian pakan terhadap pertumbuhan, komposisi tubuh (air, protein dan lemak), produksi dan komposisi karkas serta komposisi kimia daging pada domba garut jantan masa pertumbuhan. Penelitian menggunakan 12 ekor domba garut jantan dengan rataan bobot badan awal 16.76 ± 2.09 kg (Koefisien Keragaman = 12.47%), umur 6-7 bulan. Dua jenis pakan yang digunakan disusun isoprotein (14%) dan isoTDN (65%), R1 (40% rumput lapang + 60% konsentrat) dan R2 (40% limbah tauge kacang hijau + 60% konsentrat). Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) pola faktorial, 2x2. Faktor pertama adalah 2 jenis pakan (R1 dan R2), serta faktor kedua adalah waktu pemberian pakan, yaitu pagi hari (P) dan sore hari (S). Masing-masing perlakuan terdiri dari 3 ulangan. Pengaruh pakan dan waktu terhadap pertambahan bobot badan, komposisi tubuh, produksi karkas dan komponennya serta komposisi kimia daging dipelajari dengan analisis ragam. Komposisi kimia daging dan lemak karkas yang diperoleh secara pemotongan dengan komposisi tubuh yang diperoleh melalui metode ruang urea dicari hubungannya menggunakan uji korelasi-regresi. Hasil penelitian menunjukkan bahwa tidak ada interaksi antara jenis pakan dan waktu pemberian pakan terhadap produktivitas domba. Pakan mengandung limbah tauge nyata meningkatkan pertambahan bobot badan, persentase dan komposisi karkas (daging, lemak), serta menurunkan konversi pakan. Waktu pemberian pakan sore hari menghasilkan daging karkas yang sangat nyata lebih tinggi (P<0.01) daripada pagi hari. Hubungan antara bobot hidup, komposisi tubuh dengan komposisi karkas dan kimia daging adalah nyata. Hubungannya mengikuti persamaan sebagai berikut: lemak karkas (%) = 0.143 bobot hidup - 0.660; lemak karkas (%) = 4.286 lemak tubuh - 82.021 dan protein daging (%) = 424.98 - 27.32 protein tubuh. Pemberian limbah tauge dalam pakan secara nyata meningkatkan performa dan dapat dijadikan sebagai pakan alternatif pengganti rumput.
Keyword: body composition, carcass, feeding time, mung bean sprouts waste, sheep

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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