Judul: Analisis Kinerja Sistem Manajemen Basis Data NoSql (Studi Kasus Menggunakan Struktur Tabel Ijah).
Abstrak: Indonesia jamu herbs (Ijah) merupakan aplikasi yang bertujuan memprediksi dan memvisualisaikan interaksi antara tumbuhan, senyawa, protein, dan penyakit. Ijah menerapkan basis data relasional sebagai penyimpanan datanya. Secara umum basis data dibagi menjadi 2 kategori, yaitu basis data relasional dan basis data non-relasional (NoSql). Tabel pada Ijah tidak saling berelasi, sehingga penggunaan basis data relasional kurang tepat. Penelitian ini dilakukan untuk mendapatkan rekomendasi basis data yang sesuai untuk Ijah. Tiap basis data mempunyai struktur yang berbeda-beda. Tahapan yang dilakukan adalah membuat model data, perancangan kueri, analisis, dan evaluasi. Tiga kasus diujikan pada basis data PostgreSql, Cassandra, dan MongoDb. Kasus tersebut adalah memasukkan data, mengambil seluruh data, dan mengambil data pada kondisi tertentu. Hasil dari penelitian menunjukan MongoDb memiliki waktu paling cepat dalam mengeksekusi kueri.
Keyword: Basis Data, Kueri, Relasional, NoSql

Judul: Pengembangan Basis Data Ijah Analytics Menggunakan NoSQL [Studi Kasus Menggunakan Struktur Tabel Ijah].
Abstrak: Indonesia jamu herbs (Ijah) Analytics merupakan sebuah sistem yang berisi tentang pengolahan data yang saling berhubungan antara tumbuhan, senyawa, dan protein. Sistem ini dapat memprediksi khasiat jamu. Ijah Analytics menggunakan sistem basis data relasional, sehingga kurang cocok digunakan untuk penyimpanan yang bersifat non-relasional. Penelitian yang sama sebelumnya membandingkan performa antara PostgreSQL, Cassandra, dan MongoDB. Hasil yang menunjukan bahwa MongoDB memiliki hasil eksekusi kueri yang paling cepat untuk menyimpan dan mengambil data. Basis data Ijah yang semula dipresentasikan dalam bentuk model relasional dikembangkan dengan membuat model menggunakan skema basis data document store. Desain, menggunakan skema document store menghasilkan empat collection yang berisi atribut beserta key referensi yang berhubungan dengan collection lain. Evaluasi dilakukan dengan empat kondisi yaitu pengambilan data, pemasukan data, pengambilan data referensi dengan kondisi tertentu, dan pemasukan data referensi atau relasi. Evaluasi dilakukan dengan 10 kali pengulangan dengan mengolah data mulai dari 50 hingga 50000 data. MongoDB menunjukan kinerja yang lebih baik dari yang lain untuk pengambilan data, namun MongoDB tidak menunjukan performa yang baik untuk melakukan beberapa kueri dengan kondisi tertentu dan juga kueri yang kompleks.
Keyword: Bioinformatika, Basis Data, IJAH, MongoDB, NoSQL

Judul: Extracellular Expression of thermostable Geobacillus stearothermophilus T1.2RQ Lipase for Biodiesel Production
Abstrak: The extracellular expression of enzymes in an excellent secretion host such as Bacillus subtilis is a useful strategy for making enzymes of industrial significance more affordable by lowering the cost of its downstream processing. Lipases are a category of hydrolases that catalyze the hydrolysis of triglycerides to glycerol and free fatty acids. Also, lipases catalyze the hydrolysis and transesterification of other esters as well as the synthesis of esters. They can perform very specific biotransformation reactions and are widely used in food, organic synthesis, detergents, cosmetic, and pharmaceutical industries. Geobacillus stearothermophilus T1.2RQ lipase is an industrially-desired thermostable lipolytic enzyme with an excellent hydrolytic and transesterification activity. This study aimed to express T1.2RQ lipase, in B. subtilis WB800 and ensure its secretion into the extracellular medium. Three signal peptides (AmyQ, Epr, LipA) and two promoters (Pgrac01 and Pgrac100) were used in the secretory-expression of T1.2RQ lipase, based on five constructed expression vectors constructed by restriction cloning and PCR mutagenesis. Sangers sequencing was used to verify constructs to ensure they are in the correct reading frame. Recombinants vectors, pHT43 (plasmid control), pHT43-T1.2RQ, pHT43- T1.2RQ_AmyQ, pHT43-T1.2RQ_Epr, and pHT43-T1.2RQ_LipA, were transformed by electroporation into Bacillus subtilis WB800. The electroporation was successful with the optimum condition of 2500V, 200 ohms, 25µF, and 5milliseconds. Recombinant B. subtilis pHT43 (negative control) and pHT43-T1.2RQlip + signal peptides were streaked on the lipidic agar, LA+TBN. All four recombinants, including the negative control (B. subtilis WB800-pHT43), produced clear zones on the TBN agar plate after overnight incubation (fig 2), indicating the presence of a lipolytic enzyme in all of the recombinant B. subtilis cells. pHT43-T1.2RQlip + signal peptides (AmyQ, Epr, and LipA) have clear zones with significantly higher diameters than B. subtilis WB800-pHT43 (negative control). This phenomenon suggests that the recombinant thermostable lipase T1.2RQ introduced into these cells was successfully expressed, thereby increasing the lipolytic capability of its host cells T1.2RQ lipase was expressed in 50ml terrific broth medium (TB) and the expression induced with 1mM IPTG. Lipase activity assay using p-nitrophenyl esters showed that all three signal peptides directed the secretion of T1.2RQ lipase into the extracellular medium. Signal peptide, LipA, resulted in the highest extracellular activity of 5.6 U/mL, which corresponds to a 6-fold increase over the parent B. subtilis WB800 strain. Meanwhile, signal peptides, AmyQ and Epr produced 4.2 U/mL and 3.4 U/mL activity respectively. SDS-Zymogram analysis confirmed that T1.2RQ lipase was correctly processed and secreted in its original size of 44 kDa. An attempt to further increase lipase expression level by promoter optimization showed that, contrary to expectation, promoter Pgrac100 exhibited similar expression levels as the original Pgrac01. Nevertheless, the demonstration of the successful secretion of T1.2RQ lipase in this work confirms the latter as a promising approach for the industrial production of the enzyme
Keyword: Bacillus subtilis, Expression, Extracellular, Heterologous, Lipase, Thermostable