Judul: Diferensiasi Genetik Populasi Udang Jerbung (Fenneropenaeus merguiensis De Man) dari Banten, Jawa Tengah, Bengkulu, Kalimantan Barat, dan Nusa Tenggara Barat
Abstrak: Genetic differentiation (phenotype and genotype) of population is need as base information for banana prawn breeding program. Information of genetic differentiation banana prawn in Indonesia is few, so this study was purposed for determine morphologycally and molecular different banana prawn (Fenneropenaeus merguiensis) from Banten, Central Java, Bengkulu, West Kalimantan, and West Nusa Tenggara. Morphologycally measurement with morphometric method was applied for determine phenotype, and mitochondrial DNA 16S ribosomal RNA analysis with sequencing method for determine genotype different. Results of analysis showed that there were morphometrically genetic variations among banana prawn population studied. Banana prawn from West Kalimantan had highest variation of morphology and Bengkulu lowest variation. Results of Cluster analysis indicated that two main clusters were found, i.e. banana prawn from West Kalimantan, West Nusa Tenggara and Bengkulu as the first cluster, Central Java and Banten as the second cluster. PCR amplification and sequencing of 16S-rRNA mitochondrial DNA region were performed using 5’-CGCCTGTTTAAC-AAAAACAT-3’ and 5’-CCGGTCTGAACTCAGATCATGT- 3’. Analysis of homology sequences of 16S-rRNA mtDNA show that banana prawn used in this study was Fenneropenaeus merguiensis. Result of family relationship analysis indicated that 5 population of banana prawn can divided into 2 groups, i.e. West Kalimantan and group of Bengkulu-Banten-Central Java-NTB. Banana prawn from West Kalimantan and Bengkulu has specific sequences at 5’ terminal, ACTGACT and C-GAC, respectively. Those sequences may be able to be use as a marker in the breeding program of banana prawn in Indonesia.
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Judul: Analisis Keragaman Genetik Ikan Baung (Hemibagrus nemurus) dengan Menggunakan Teknik PCR-RFLP pada DNA Mitokondria
Abstrak: This research has an objective to analyze the genetic variation among four Hemibagrus nemurus population by using PCR-RFLP of D-loop region of mitochondrial DNA. H. nemurus were collected from Cirata, Serayu, Jambi and Bogor. Four restriction enzymes were used to digest D-loop region mt-DNA, i.e. MboI, HaeIII, AluI and RsaI. Based on this analysis, these four population have seven composite haplotypes. The highest haplotype diversity was found at the population from Jambi. Population from Serayu has just one composite haplotype indicating that this population has the same ancestor. Population from Cirata and from Bogor has the same composite haplotype indicating that there was probably migration between these two populations.
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Judul: Pembuatan Film Tipis Cu2ZnSnS4 sebagai Lapisan Penyerap Cahaya pada Sel Surya Menggunakan Metode Elektrodeposisi
Abstrak: Thin film of Cu2ZnSnS4 (CZTS) is an alternative semiconductor used as light absorber layer on solar cell. CZTS can replace silicon that commonly used as commercial semiconductor. The advantage of using CZTS as absorber layer is due to the abundance of its chemical composer on earth, and its high value of absorption coefficient. The absorber layer was made by using electrodeposition method on ITO glass substrates. The films were deposited at room temperature using potentiostatic mode with a potential of –1.05 V for 30, 45, and 60 minutes. After that, the films were annealed at two different temperatures, 180 ºC and 500 °C, for 45 minutes in N2/H2S atmosphere. Based on X-ray diffraction analysis, the polycrystalline thin film obtained fall into kesterite crystal phase. This type of crystal was obtained when the CZTS was annealed at 500 °C in H2S/N2 atmosphere. The value of optical band gap energy for the CZTS thin film was 2.2 eV with the film thickness about 1.228–6.366 μm. The sample of CZTS with electrodeposition time of 45 minutes and annealing temperature of 500 °C showed the best result and in conformity with absorber layers criteria for solar cells.
Keyword: semiconductor, ITO glass, CZTS thin film, electrodeposition