Judul: Uji Potensi Transmisi Vaksin Gp25 pada Bakteri Flora Normal Media Budidaya Ikan Mas Cyprinus Carpio Secara In Vitro
Abstrak: Koi herpesvirus (KHV) merupakan virus yang menginfeksi ikan mas dan koi. KHV menyerang hampir semua stadia ikan mas dan koi dan menyebabkan kematian 80-95% populasi. Salah satu cara penanggulangan penyebaran KHV adalah dengan penggunaan vaksin DNA. Penelitian ini dilakukan untuk menguji potensi transmisis vaksin DNA Glikoprotein 25 (GP25) pada bakteri flora normal pada media budidaya ikan mas Cyprinus carpio. Bakteri asal kolam budidaya ikan mas diisolasi dan diuji sensitivitasnya terhadap antibiotik ampisilin. Penelitian dengan menambahkan bakteri sensitif ampisilin dengan vaksin GP25 sebanyak 100 μl dengan dosis 12,5 μg/100 μl dan diinkubasi pada suhu 28º C selama 30, 60, 180, dan 300 menit kemudian disebar pada media mengandung antibiotik ampisilin. Pertumbuhan bakteri yang terjadi diuji keberadaan gen pembawa plasmid yang mengandung penanda resisten terhadap ampisilin dalam tubuh bakteri menggunakan metode seleksi koloni bakteri ( Cracking sel). Hasil penelitian ini menunjukan bahwa tidak ada bakteri yang mengandung gen pembawa plasmid GP25 ditandai dengan tidak terdeteksi pita gen pembawa plasmid pada metode Seleksi Koloni Bakteri (Cracking sel), elektroforesis dan PCR.
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Judul: Persistensi vaksin DNA penyandi glikoprotein 25 yang diberikan melalui pakan buatan pada ikan mas (Cyprinus carpio)
Abstrak: Koi Herpes Virus (KHV) is a major viral pathogen that infects common carp and koi. KHV disease outbreak in almost all centre of common carp culture in Indonesia and caused mass mortality. DNA vaccination method is one of ways to cope with KHV infection. Vaccines were commonly given by injection. In this research, persistence test of DNA vaccine (pMBa-GP25) were carried out by oral feeding on common carp. DNA vaccine persistence test were done using PCR method with the specific primer for GP25 gene. Escherichia coli DH5α bacteria containing pMBa-GP25 DNA vaccine were cultured in four treatment mediums namely 2xYT, LB polypeptone, LB triptone and TSB. Bacteria densities were counted with spectrophotometer using 580 nm wave lengths. The results showed that an efficient culture media for E. coli was LB tripton. Furthermore, GP25 DNA was detected in kidney and spleen of vaccinated fish. Level of DNA vaccine that could be detected was 7,56 ng (amount of DNA copy 1,598 x 1010 copies).
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Judul: Truncated Stop Loss as a Optimal Solution of Reinsurance Agreement in One-Period Model
Abstrak: Reinsurance is one of risk management efforts of an insurance company, which is called the cedent. Similar with insurance, reinsurance constitutes the cedent to pay the premium. We consider one-period reinsurance model and derive a rule which minimizes the ruin probability of the cedent for a fixed reinsurance premium. Therefore, it is needed to construct an optimal reinsurance agreement for the cedent. The premium is calculated according to four different principles, i.e. economic, generalized zero-utility, Esscher, and mean-variance principles. Stop loss is a reinsurance contract which protect the insurance company for losses that exceed a certain amount. The result of this research shows that a truncated stop loss is an optimal agreement in the class of all reinsurance contracts with various premiums calculated using above mentioned principles.
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