Judul: The Effectiveness of Indole-3-Butyric Acid (IBA) on the Growth of Mutant Roots of Alfalfa (Medicago sativa L.) Acid-Resistant pH 3.6 in In Vitro Culture
Abstrak: Alfalfa (Medicago sativa L.) is a type of legume forage that has the potential to be used as a source of animal feed. Alfalfa has a high protein content and is good as animal feed. Alfalfa plant mutants resistant to pH 3.6 have been produced, a collection of Pastura Laboratory, Department of Nutrition and Feed Technology, Faculty of Animal Husbandry, IPB University with limited seed production capabilities. One of the efforts to cultivate and multiply the culture of alfalfa plant collections that are pH 3.6 resistant is by vegetative propagation through tissue culture. Tissue culture is a plant cultivation technique that is carried out in vitro. The tissue culture technique has several stages in the process, namely explant culture initiation, propagation, rooting and plant acclimatization. Root formation can be stimulated more rapidly by auxin as a growth regulator. The purpose of this study was to determine the effectiveness of IBA on root growth of alfalfa (Medicago sativa L.) mutant acid resistance pH 3.6 through tissue culture techniques. The method used in this study was a completely randomized design (CRD) with 5 treatments, namely control, IBA 0.5 ppm, 1.0 ppm, 1.5 ppm and 2.0 ppm. The data obtained were analyzed using Analysis of Variance (ANOVA) with SPSS version 16.0 and the difference in significance between treatments was further analyzed using Tukey's test. The variables observed were root length, number of rooted plants, plant height, number of leaves and shoots, leaf fall, plant mortality, plant biomass and media loss. The results showed that the number of rooted plants and the increase in root length of alfalfa plants gave a good response to 2.0 ppm. The conclusion of this study is the effective level of adding IBA to mutant plants Alfalfa (Medicago sativa L.) acid resistant pH 3.6 is 2.0 ppm. Increased level of compassion also had a positive correlation with root growth, leaf formation and vertical height gain but had a negative correlation with shoot formation, biomass and media shrinkage., Alfalfa (Medicago sativa L.) merupakan salah satu hijauan jenis leguminosa yang berpotensi untuk dimanfaatkan sebagai sumber pakan ternak. Alfalfa memiliki kandungan protein yang tinggi dan baik sebagai pakan ternak. Telah dihasilkan mutan tanaman alfalfa tahan pH 3.6 koleksi Laboratorium Pastura, Departemen Ilmu Nutrisi dan Teknologi Pakan, Fakultas Peternakan, Institut Pertanian Bogor dengan kemampuan produksi biji yang terbatas. Salah satu upaya pembudidayaan dan perbanyakan kultur koleksi tanaman alfalfa tahan pH 3.6 adalah dengan perbanyakan vegetatif melalui kultur jaringan. Kultur jaringan merupakan teknik budidaya tanaman yang dilakukan secara in vitro. Teknik kultur jaringan memiliki beberapa tahapan dalam prosesnya, yaitu inisiasi kultur eksplan, perbanyakan, perakaran dan aklimatisasi tanaman. Pembentukan akar dapat dirangsang lebih cepat oleh auksin sebagai pengatur tumbuh. Tujuan penelitian ini adalah untuk mengukur efektifitas IBA terhadap pertumbuhan akar mutan tanaman alfalfa (Medicago sativa L.) tahan asam pH 3.6 melalui teknik kultur jaringan. Metode yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) dengan 5 perlakuan yaitu Kontrol, IBA 0.5 ppm, 1.0 ppm, 1.5 ppm dan 2.0 ppm. Data yang diperoleh dianalisis menggunakan Analysis of Variance (ANOVA) dengan program SPSS versi 16.0 dan perbedaan signifikansi
Keyword: Alfalfa (Medicago Sativa L.), IBA, Issues culture

Judul: Response of Colchicine on the Plant Growth of Alfalfa (Medicago sativa L.) in Tissue Culture
Abstrak: Tanaman Alfalfa (Medicago sativa L.) merupakan hijauan pakan ternak jenis leguminosa yang memiliki produktivitas dan kandungan protein yang tinggi. Penelitian ini bertujuan menganalisis respon pertumbuhan tanaman Alfalfa (Medicago sativa L.) dengan pemberian kolkisin. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 20 ulangan. Perlakuan yang digunakan adalah P0=0 ppm, P1=0.5 ppm, P2=1 ppm, P3=1.5 ppm; dan P4=2 ppm. Data dianalisis menggunakan analisis deskriptif dan analisis varian (ANOVA) serta diuji lanjut menggunakan uji lanjut Duncan. Peubah yang diamati antara lain tinggi vertikal tanaman, panjang daun, jumlah daun, respon warna daun, kerontokan daun, kontaminasi, penyusutan media dan biomassa. Hasil analisis ragam menunjukkan parameter panjang daun tumbuh baik pada konsentrasi kolkisin 0.5 ppm dan 1 ppm. Simpulan dari penelitian ini adalah tanaman Alfalfa memberikan respon pertambahan panjang daun yang tertinggi pada pemberian kolkisin dengan konsentrasi pemberian 0.5 ppm dan 1 ppm. Kolkisin juga memberikan pengaruh terhadap perubahan warna daun yang lebih gelap. Pemberian kolkisin hingga 2 ppm tidak memberikan pengaruh terhadap pertambahan tinggi vertikal tanaman, jumlah daun, kerontokan daun, penyusutan media, biomassa dan kontaminasi., Alfalfa (Medicago sativa L.) is a forage plant that has high productivity and protein content. This study aimed to analyze the growth response of Alfalfa (Medicago sativa L.) plants with colchicine. This study used a completely randomized design (RAL) with 5 treatments and 20 replications. The treatments used were P0=0 ppm, P1=0.5 ppm, P2=1 ppm, P3=1.5 ppm, and P4=2 ppm. Data were analyzed using descriptive analysis and analysis of variance (ANOVA) and tested using Duncan's test. The variables observed were plant height, leaf length, number of leaves, leaf color response, leaf loss, contamination, media shrinkage and biomass. The results of the analysis of variance showed that the leaf length response grew well at 0.5 ppm and 1 ppm colchicine concentrations. The conclusion of this research is that the response of colchicine to Alfalfa showed optimal leaf length increase at 0,5 ppm and 1 ppm colchicine concentrations. The administration of colchicine also gave a positive trend towards increasing darker leaf color. The administration of colchicine up to 2 ppm had no effect on the increase of plant vertical height, number of leaves, leaf loss, media shrinkage, biomass and contamination.
Keyword: alfalfa, colchicine, tissue culture, response

Judul: Penggunaan Teorema Homeomorphy 2-Manifold dan Teorema Euler Poincare pada Torus 𝑇�� dan Simplicial Complex 𝐾��
Abstrak: Two dimensional topological spaces are said to be homeomorphic if they have the same topological invariant, where one of topological invariant used is an Euler characteristic. Homeomorphy 2-Manifold’s Theorem and Euler Poincare’s Theorem are used to distinguish two topological spaces. Homeomorphy 2-Manifold’s Theorem uses Euler characteristic to identify two dimensional topological spaces. Euler Poincare’s Theorem is an alternative way to find Euler characteristic with Betti number, which is topological invariant as well. The objective of this paper is to investigate the homeomorphism of torus 𝑇����� and simplicial complex 𝐾�����. Topological space torus and simplicial complex 𝐾����� have the same Euler characteristic, which is two. Based on Homeomorphy 2-Manifold’s Theorem topological space torus 𝑇����� and simplicial complex 𝐾����� is homeomorphic.
Keyword: simplicial, torus, Euler, homeomorphism, topology