Judul: Analisis Empirical Orthogonal Function (EOF) dan Transformasi Fourier pada Sinyal Curah Hujan Indonesia.
Abstrak: Curah hujan merupakan parameter atmosfer yang sulit diprediksi karena memiliki keragaman yang tinggi baik secara spasial dan temporal. Tujuan penelitian ini adalah menganalisis frekuensi sinyal curah hujan enam bulanan dan pola curah hujan harian Indonesia secara spasial dan temporal untuk mendapatkan variasi diurnal. Analisis transformasi Fourier digunakan untuk mengetahui pengaruh sinyal curah hujan enam bulan terhadap curah hujan Indonesia pada data TRMM 3B43. Hasil analisis menunjukkan wilayah Indonesia yang terbagi menjadi tiga tipe hujan memiliki kekuatan sinyal curah hujan enam bulanan yang berbedabeda dengan urutan kekuatan sinyal berturut-turut monsoonal, ekuatorial, kemudian lokal. Analisis EOF berbasis dekomposisi nilai singular digunakan untuk mereduksi data TRMM 3B42 dan mendapatkan pola-pola dominan secara spasial dan temporal. Hasil analisis pada tiga pola dominan utama dari 2920 pola menunjukkan pola dominan pertama didominasi oleh pola hujan enam bulanan, sedangkan variasi diurnal diperoleh pada pola dominan kedua dan ketiga.
Keyword: curah hujan TRMM 3B42,, curah hujan TRMM 3B43, dekomposisi nilai singular, empirical orthogonal function (EOF), transformasi Fourier

Judul: Penggunaan Empirical Orthogonal Function (EOF) untuk Identifikasi Karakteristik Curah Hujan (Studi Kasus: DAS Ciujung-Cidurian)
Abstrak: Rainfall in Indonesia is highly varied in space and time. The purpose of this research was identify spatial and temporal rainfall pattern, examine impact of ENSO on rainfall variability, and obtain the location of stations which had great impact on rainfall variability. The method used Empirical Orthogonal Function (EOF) and Rotated Empirical Orthogonal Function (REOF). From EOF obtained annual and seasonal rainfall variance dominated by monsoon system. Type of mountain topography also contributed on rainfall variability. EOF seasonal temporal patterns related with ENSO. It was seen from high value of correlation between EOF coefficient and SOI. Correlation between EOF1 with MAM, JJA, and SON period respectively -0.467, 0.413, and 0.32. EOF2 correlated with DJF and SON period with correlation respectively -0.555 and -0.588. Rotated Empirical Orthogonal Function (REOF) was used to see the contribution of each observation station by rotating the EOF. Type of rotation is varimax rotation.The result of rotation showed factor1 was determined by two station, Ragas Hilir and Jongjing station whereas other factors were determined by one stasion only.
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Judul: Gene Editing  of Two Cyanide Synthesis-related Genes in Cassava using CRISPR/Cas9
Abstrak: Cassava (Manihot esculenta) contain toxic quantities of cyanogenic glucoside. Reducing the potentially toxic cyanogenic glucoside levels in cassava could be achieved by gene editing through Clustered Regularly Interspaced Short Palindromic Repeats – CRISPR associated protein 9 (CRISPR/Cas9), a high- precision genome editing technique in plants. Two cassava UDP- glucosyltransferase (UGT) paralogs, UGT85K4 and UGT85K5, are the genes catalyzing the synthesis of cyanogenic glucosides in plants. The study aimed to construct the CRISPR/Cas9 recombinant vector carrying single guide RNA (sgRNA) to edit UGT85K4 and UGT85K5 genes, and transform sgRNA CRISPR/Cas9 construct into KU50 cassava calli. Five sgRNAs were designed, considering the on/off target efficiency score and GC content. Those sgRNAs were ligated into pRGEB32 plasmid and introduced to competent Escherichia coli DH5a. The PCR colony and DNA sequencing were performed to verify the sgRNA insert. The amplicons showed the expected band size of recombinant vector (180 bp). Based on sequencing results, only one sgRNA was successfully ligated into the plasmid that will be used to edit the UGT85K5 gene. The sgRNA CRISPR/Cas9 construct in pRGEB32 plasmid was successfully transformed into Agrobacterium tumefaciens and introduced into KU50 cassava calli through co-cultivation. The overgrowth of Agrobacterium occurred after the co-cultivation phase.
Keyword: CRISPR/Cas9, Cyanogenic glucoside, Gene editing, sgRNA