Judul: judul karya ilmiah
Abstrak: abstract...
Keyword: keywords1, keywords2, keywords3

Judul: Text mining Dokumen Skripsi berdasarkan Abstrak dengan Menggunakan Metode Gerombol Berhirarki dan Latent Dirichlet Allocation
Abstrak: Abstrak merupakan ringkasan dari suatu karya ilmiah yang menggambarkan isi dari suatu karya ilmiah seperti skripsi. Pengelompokan skripsi berdasarkan abstraknya ke dalam kategori-kategori salah satunya dapat menggunakan text mining. Text mining merupakan salah satu variasi dari data mining, yaitu menggali pola atau informasi penting dari data berupa teks. Penelitian ini menggunakan analisis gerombol berhirarki untuk mendapatkan gerombol-gerombol yang dihasilkan dari data teks. Analisis gerombol berhirarki yang dilakukan menggunakan metode Ward dan metode pautan lengkap serta menggunakan ukuran cosine similarity untuk mengukur jarak antar vektor dokumen. Gerombol yang dihasilkan ditentukan oleh hasil pemotongan dendogram dengan melihat nilai silhouette coefficient yang paling besar. Gerombol yang dihasilkan dari keseluruhan data diperoleh sebanyak 12 gerombol. Karakteristik masing-masing gerombol berupa kata-kata yang merupakan kata kunci atau topik dari dokumen yang termasuk dari gerombol tersebut. Metode Latent Dirichlet Allocation mengambil kata-kata dari keseluruhan kata pada dokumen yang bisa menjadi topik-topik dari 12 gerombol tersebut.
Keyword: Bogor Agricultural University, Institut Pertanian Bogor, IPB, Text mining, Abstract, Ward, Complete linkage, Lantent dirichlet allocation, Pautan lengkap, Pengelompokan dokumen, Skripsi, Metode analisis gerombol, Abstrak skripsi

Judul: Heterologous Expression Of Endo-Β-Xylanase From Bacillus Pumilus Strain Bcrs-01 In Escherichia Coli.
Abstrak: Xylanases are glycoside hydrolase family 11 which depolymerise xylan in the plant cell wall, the most excessive form of hemicellulose. Endo-β-xylanases (EC 3.2.1.8) are most important to breakdown β-1,4-glicoside bond of xylan to produce disaccaride xyloolygosaccaride (XOs) and xylose (monomer). Bovine rumen fluids have potential to obtain endo-β-xylanase produced of bacteria. The objectives of this study were to obtain xylanase producing bacteria and to express xynA gene encoding endo-β-xylanase from bacterium in Escherichia coli. Twenty nine isolate bacteria have been succesfully isolated from bovine rumen fluid in agar medium containing birchwood xylan. Isolate BCRS-01 bacterium was previously screened for the high production of xylanase. Based on 16S rRNA gene sequence analysis, BCRS-01 showed high similarity (99%) to Bacillus pumilus (accession number NR043241). The crude xylanase had optimal activity at 55oC, pH 7.0. Furthermore, the crude xylanase exhibited broad pH stability from 4.0 to 10.0 and remained more than 60% and 40% of its activity after incubation at pH 4.0 until 10.0, respectively for 60 min. It also showed a stability at temperature 25-50oC since it retained 80% and 50% of its activity when incubated at 50oC or 30 and 60 min, respectively. The xylanase activity was strongly inhibited by Ca2+ and Cu2+ at concentration 10 mM, and as little as 1 mM for Mn2+. Therefore, this xylanase has potentially used as additive feeds in animal feed industry. The DNA sequence xynA gene encoded endo-β-xylanase was amplified and cloned into the expression vector pET-15by under the control of T7 promoter. The recombinant protein had succesfully expressed in Escherichia coli BL21 (DE3) pLysS. A protein recombinant of approximately, 26.7 kDa was observed on the SDS-PAGE analysis. Crude extract of recombinant endo-β-xylanase demonstrated activity of enzyme at 56 U mL-1 using birchwood xylan as a substrate at 55oC for 10 min.
Keyword: Bacillus pumilus, bovine rumen fluid, endo-β-xylanase, heterologous expression.