Judul: Konsep Pola Permukiman Suku Baduy Dalam di Desa Kanekes, Kabupaten Lebak, Banten.
Abstrak: Suku Baduy yang bermukim di Desa Kanekes merupakan salah satu masyarakat yang memegang erat adat Sunda. Desa Kanekes terletak pada wilayah Kecamatan Leuwidamar, Kabupaten Lebak, Banten. Terdapat tiga desa utama dalam Desa Kanekes, yakni Kampung Cikeusik, Cikartawarna, dan Cibeo. Masyarakat Baduy berpegang teguh terhadap adat leluhur sehingga membentuk suatu kelompok yang terisolasi dari dunia luar. Kearifan lokal yang dimiliki oleh Suku Baduy terhadap alam, kesederhanaan, dan toleransi akan lingkungan sekitar mempengaruhi terbentuknya suatu budaya dari masyarakat baduy itu sendiri. Tatanan ruang Suku Baduy terbentuk karena adanya budaya yang mempengaruhinya sehingga membentuk suatu pola permukiman dengan aturan yang dipegang teguh. Penelitian ini dilakukan dengan menggunakan metode deskriptif melalui pengambilan informasi terkait budaya dan sejarah dari dokumen adat atau naskah adat dan wawancara melalui tokoh adat atau keyperson dan melakukan verifikasi melalui pengamatan langsung dengan observasi tapak. Penelitian ini mampu mengidentifikasi elemenelemen pembentuk lanskap permukiman tradisional Baduy, yaitu bangunan (rumah puun, rumah jaro, rumah warga, bale, bale panjang, leuit, dan saung lesung), ruang terbuka (alun-alun, leuweung lembur, huma, reuma, dan leuweung kolot), aksesibilitas (jalan utama), air (sungai dan pancuran), serta vegetasi (leuweung lembur, huma, reuma, dan leuweung kolot). Pola permukiman pada ketiga kampung di Baduy Dalam memiliki tata letak yang sama, yaitu berbentuk asimetris dengan arah utara dan selatan sebagai axis.
Keyword: DesaKanekes, pola permukiman, SukuBaduy, tata ruang

Judul: Sistem Tenurial dan Kearifan Tradisional Masyarakat Adat Baduy dan Hubungannya dengan Sistem Pangan Lokal (Kasus Desa Kanekes, Kecamatan Leuwidamar, Kabupaten Lebak, Provinsi Banten)
Abstrak: Peningkatan jumlah penduduk mengakibatkan semakin tingginya kebutuhan terhadap lahan untuk produksi pangan. Penelitian ini dilakukan pada masyarakat Baduy Desa Kanekes, Kecamatan Leuwidamar, Kabupaten Lebak, Provinsi Banten. Tujuan dari penelitian ini adalah menganalisis sistem tenurial, kearifan lokal dalam sistem pertanian, hubungan sistem tenurial dan kearifan lokal terhadap sistem pangan lokal. Metode yang digunakan dalam penelitian ini adalah pendekatan kuantitatif didukung oleh data kualitatif dan teknik non-probability sampling dalam menentukan responden. Unit analisis dalam penelitian ini adalah rumah tangga dengan jumlah sampel sebanyak 40 responden yang dipilih secara purposive. Hasil penelitian menunjukkan bahwa sistem kepemilikan lahan pada masyarakat Baduy diperoleh melalui pewarisan dan pembelian lahan, sementara sistem penguasaan lahan diperoleh melalui sewa dan bagi hasil. Bentuk budidaya dalam pertanian masyarakat Baduy adalah tanaman campuran dengan pola tanam secara tumpang sari. Hasil panen padi secara budaya tidak boleh diperjualbelikan namun harus disimpan di dalam leuit (lumbung padi). Kearifan lokal berhubungan dengan sistem pangan lokal masyarakat Baduy. Sedangkan sistem tenurial tidak berhubungan dengan sistem pangan lokal masyarakat Baduy., The increase in population has resulted in a higher demand for land. The location of this study was in the Kanekes Village, Leuwidamar District, Lebak Regency, Banten Province. This study aims to determine tenure system, local wisdom in the agricultural system, the effect of tenure system and local wisdom on the local food This study used a quantitative with qualitative approach and used non-probability sampling techniques. The unit of analysis in this study was households with a sample of 40 respondents who were selected purposively. The results showed that the land tenure system in the Baduy community was through inheritance, buying and selling, renting, and profit-sharing. The form of cultivation in Baduy agriculture is mixed crops with an intercropping pattern. Rice yields are culturally not allowed to be traded but must be stored in leuit (rice barns). Local wisdom related to the local food system of the Baduy community. Meanwhile, the tenure system is not related to the local food system of the Baduy community. Keywords: Baduy
Keyword: kearifan tradisional, masyarakat Baduy, sistem tenurial, tenure system, traditional wisdom, Baduy community

Judul: Kloning dan Ekspresi Human Interferon-alfa2a pada Yeast Metilotropik Pichia pastoris
Abstrak: Interferon (IFN) is a group of proteins that are secreted by body cells as a result of exposure to biological vertebrates such as: viruses, bacteria, protozoa and other compounds. There are three types of interferon, namely: alpha (α), beta (β) and gamma (γ) IFN. Interferon-α2a belongs to the type I interferon. This protein has a molecular weight of 21.550 kDa and consists of 188 amino acids (23 amino acid of signal peptide and 166 amino acids of mature protein). Interferon-α2a is also known to be used for the treatment of hepatitis C and as anti-tumor and antivirus agents. Regulator gene for IFN production under normal conditions, is in the off position to IFN is not produced. But when there are external stimuli, both viral and bacterial infections, a regulator gene switches on, and IFN production system running. At the time of an attack from a variety of disease agents actually the body will produce IFN, but generally the amount produced is not sufficient to fight the disease agents that multiply very quickly. Therefore IFN intake from outside is needed. This is the beginning of the use of IFN as a drug. Genetic engineering is an activity to manipulate the genes to get new products by creating recombinant DNA through gene insertion. Recombinant DNA technology has been used to produce IFN. Yeast such as Pichia pastoris can be an alternative as the host (system) to express human interferon genes and as a producer of IFN protein. Expression system P. pastoris offers several advantages for the production of recombinant proteins, such as: the high level expression of recombinant proteins, ease of scaling-up fermenter, low cost and ease of transformation techniques and selection of transformed cells such as bacteria . The existence of inducible AOX1 promoter is very strong, easily control the expression of recombinant proteins by induction. AOX1 is a promoter that controls gene expression of alcohol oxidase to metabolize methanol as carbon source of P. pastoris. Therefore,this study aimed to clone human interefron-α2a (hifnα2a) gene in E. coli and express it in metylotrophic yeast P. pastoris. Vector used in this study is pPICZαB (Invitrogen). Ligation process between insert (hifn-α2a gene) and vector (pPICZαB) performed by using T4 DNA ligase, which functions synthesize the formation of phosphodiester bonds linking the nucleotides with nucleotide insert plasmid vectors to produce recombinant (pPICZαB-hifnα2a). This recombinant plasmid transformed into XL1 blue cells (E. coli) by Heat shock method. The results of sequencing analysis (clone 6) using 5'AOX and 3'AOX primers indicate that DNA base sequence of human IFN-α2a genes has been subclone into pPICZαB vector (in frame). Recombinant plasmid (clone 6) linearized using BstX1 enzyme before transformed into X33 yeast cell. Transformation into cell was done by Electroporation. The X33 cell P. pastoris strain was used to express human interferon-α2a. X33 is strains with the phenotype Mut+ (methanol utilization plus) and can be used for positive transformants selection using zeocin. Phase protein production work using Mut+ strain occurred in two phases. The first phase is the use of glycerol to obtain a certain amount of biomass (high cell
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