Judul: Redesign of Lembah Gurame Park as an Educational Based Urban Park in Depok City
Abstrak: Taman kota merupakan ruang terbuka hijau publik yang memiliki peran sebagai tempat rekreasi, edukasi, dan aktivitas masyarakat sehingga keberadaannya perlu mampu memfasilitasi setiap kegiatan tersebut. Taman Lembah Gurame memiliki luas sekitar 3 hektar merupakan taman kota yang berfungsi sebagai ruang terbuka hijau publik di Kota Depok. Namun, fasilitas yang tersedia dinilai masih kurang memfasilitasi pengguna seperti, kurangnya tempat duduk, kurangnya fasilitas bagi penyandang disabilitas, dan kurangnya lahan parkir. Selain itu, terdapat beberapa masalah terkait penataan taman seperti vegetasi yang belum tertata rapih dan banyaknya elemen yang rusak pada area timur taman sehingga sepi pengunjung. Keberadaan Taman Lembah Gurame yang berdekatan dengan bangunan sekolah-sekolah membuat sebagian besar pengunjungnya merupakan anak-anak sehingga taman berpotensi untuk dikembangkan menjadi taman kota berbasis edukasi. Tujuan dari penelitian ini adalah untuk mengidentifikasi dan menganalisis fisik, biofisik, dan karakter taman, menganalisis aktivitas edukasi pengunjung, serta menyusun rekomendasi desain taman kota berbasis edukasi. Metode yang dilakukan dalam penelitian ini mencakup tahap persiapan, pengumpulan data, analisis dan sintesis, serta membuat konsep dan desain. Pengembangan tapak menjadi taman kota berbasis edukasi berfokus pada kegiatan seperti observasi alam, permainan edukatif, dan pembelajaran di luar ruangan. Konsep dasar yang diterapkan adalah "Experience the living masterpiece of nature's canvas," yang bertujuan menjadikan tapak sebagai fasilitas untuk rekreasi, edukasi, dan konservasi, dengan memberikan pengalaman langsung bagi pengunjung untuk menikmati alam. Desain yang diterapkan diadaptasi dari bentuk buah belimbing yang dimaknai sebagai kelima indra dan ditransformasi menjadi pola geometrik-organik. Pengembangan ruang terbagi menjadi edukasi, rekreasi, penerimaan, pelayanan, dan konservasi. Ruang edukasi terdiri dari edukasi aktif dan edukasi pasif. Ruang tersebut berfungsi sebagai ruang dengan fasilitas yang mendukung aktivitas edukasi. Aktivitas edukasi mencakup belajar di alam dan observasi vegetasi serta satwa. Pengembangan ini diharapkan memberikan masukan dalam mengembangkan taman kota berbasis edukasi., The urban park serves as a public green open space for recreation, education, and other community activities so that its facilities needs to support these function. Lembah Gurame Park, covering approximately 3 hectares, is a significant green space in Depok City. However, it currently lacks adequate seating, facilities for people with disabilities, and sufficient parking. Additional issues include poorly organized vegetation and numerous damaged elements in east area of the park, leading to lack of visitors. The existence of Lembah Gurame Park which is adjacent to school buildings makes most of the visitors are children so the park has the potential to be developed into an education-based urban park. The purpose of this research is to identify and analyze the physical, biophysical, and characteristics of the park, analyze the educational activities of visitors, and develop recommendations for the design of education based urban parks. The method used in this research includes the preparation stage, data collection, analysis and synthesis, and making concepts and designs. The site transformation into an educational urban park focuses on activities like nature observation, educational play, and outdoor learning. The fundamental design concept of Lembah Gurame Park is "Experience the living masterpiece of nature's canvas," aiming to make the site as a facility for recreation, education, and conservation, providing visitors with direct experiences to enjoy the nature. The design is inspired by
Keyword: Desain Lanskap, taman kota, Ruang Terbuka Hijau Publik, Redesain, Taman Edukasi

Judul: Eco-Design of Merdeka Park as an Alternative to the Development of Urban Green Open Space
Abstrak: Taman Merdeka berperan penting secara sosial dan ekologis di tengah padatnya Kecamatan Sukmajaya. Taman ini membutuhkan desain yang mampu menyediakan ruang yang indah dan memenuhi kebutuhan pengunjung tanpa mengesampingkan fungsi ekologisnya. Dengan demikian, desain Taman Merdeka perlu dilakukan dengan pendekatan desain ekologis yang mengurangi dampak negatif terhadap lingkungan. Penelitian ini bertujuan mengidentifikasi dan menganalisis kondisi existing tapak, menyusun konsep desain ekologis Taman Merdeka, dan mendesain secara ekologis Taman Merdeka Depok yang indah, fungsional, dan ramah lingkungan. Penelitian ini dilaksanakan menggunakan metode deskriptif melalui empat tahap yang mencakup persiapan, inventarisasi, analisis dan sintesis, dan desain dengan pendekatan fisik dan biofisik, sosial, dan desain ekologis. Konsep dasar dari desain ekologis Taman Merdeka adalah Urban Eco-Excursion yang berarti sebuah ruang terbuka hijau yang mampu mengintegrasikan berbagai kegiatan, untuk berbagai kelompok, dengan pendekatan yang ramah lingkungan. Konsep desain dikembangkan dari motif Batik Depok. Desain ekologis diterapkan melalui penggunaan green infrastructure untuk stormwater infiltration and cleaning, konservasi vegetasi eksisting, upcycling center untuk pengelolaan sampah, dan penggunaan bahan yang ramah lingkungan. Desain taman mencakup bioswales, stormwater wet pond, lapangan-lapangan olahraga, playground, plaza, area pujasera, upcycling center, welcome area, area duduk, elevated walkway, amphitheatre, lawn, promenade, dan toilet., Merdeka Park plays important social and ecological roles in the midst of the very dense Sukmajaya District. This park needs a design that is able to provide an aesthetically pleasing space and meet the needs of visitors without neglecting ecological functions. Thus, the design of Merdeka Park needs to be done with an ecological design approach that minimizes the impacts that may damage the environment. This study aims to identify and analyze the existing site conditions, create an ecological design concept of Merdeka Park, and ecologically design Merdeka Park that is beautiful, functional, and environmentally friendly. This study was conducted using descriptive method through four stages which are preparation, inventory, analysis and synthesis, and design with a physical and biophysical, social, and ecological approaches. The basic concept for the eco-design of Merdeka Park is Urban Eco-Excursion which means a green open space that integrates various activities for various groups with an environmentally friendly approach. The design concept is inspired by Depok's Batik. Eco-design is implemented through the use of green infrastructure for stormwater infiltration and cleaning, conservation of existing vegetation, upcycling center for waste management, and environmentally-friendly materials. The design of the park include bioswales, stormwater wet pond, sports fields, playground, plaza, food court, upcycling center, welcome area, seating areas, elevated walkway, amphitheatre, lawn, promenade, and toilet.
Keyword: green infrastructure, recycling, upcycling, urban park

Judul: Pemurnian dan karakterisasi protein inhibitor RNA helikase virus hepatitis C
Abstrak: Hepatitis C virus (HCV) infection is the second most common chronic viral infection in the world with global prevalence. This infection is contribution to chronic hepatitis and liver cirrhosis, often leading to hepatocellular carcinoma. There is no effective drug and vaccine available against HCV. The current standard treatment, a combination of pegylated interferon-α with ribavirin, is limited by its suboptimal response rate in a significant patient population, side effects, and affordability. Therefore, development of effective drug is needed. One approach to find out the candidate of antiviral drug is to discover the inhibitor of enzyme that essential for virus replication, such as RNA helicase. Streptomyces chartreusis 5-095 produce the inhibitor of HCV RNA helicase. The aim of this study was to purify the protein inhibitor which has inhibition activity against ATPase activity of RNA helicase HCV from S. chartreusis 5-095. The supernatant of culture S. chartreusis 5-095 exhibited high inhibiton activity against ATPase activity of RNA helicase HCV (approximately 72%) at 10 days of incubation. The extracellular protein inhibitor was partially purified by procedures of precipitation with ammonium sulfate to 65% saturation and gel filtration (Sephadex G50) using methanol water solution (4:6) as an eluen. The purity of protein inhibitor was monitored by SDS PAGE. The protein inhibitor showed a relative molecular mass were estimated to be 14 kDa and increased the purity 23,08 fold than those of the crude protein. The specific activity and IC50 after purification were 13,1338 U/mg and 0,0767 mg/mL, respectively. The activity of purified protein inhibitor showed its stability over pH range from 5-8 and optimum at pH 7,4. The inhibition activity showed its stability up to 70% at temperature 100 oC. The purified protein inhibitor relatively stable its activity at storage in the range of -20 oC to room temperature for 35 days.
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