Judul: Effects of LED (Light-Emitting-Diode) Spectrum and Intensity on Strawberry (Fragaria x ananassa Duch.) Plant Physiology and Secondary Metabolite Profiles in Lowlands
Abstrak: Stroberi (Fragaria x ananassa Duch.) merupakan tanaman asal iklim subtropis yang dapat dibudidayakan di daerah beriklim tropis. Upaya penyeseuaian lingkungan tumbuh asal tanaman dapat dilakukan dengan penggunaan LED sebagai cahaya buatan. Penelitian bertujuan mengetahui respons fisiologi dan karakterisasi metabolit sekunder terhadap stroberi yang diberikan perlakuan LED. Penelitian menggunakan rancangan kelompok lengkap teracak (RKLT) yang terdiri atas 2 faktor yaitu spektrum dan intensitas cahaya. Faktor spektrum terdiri atas 6 taraf yaitu merah 100%, biru 100%, merah 75% biru 25%, merah 25% biru 75%, merah 50% biru 50%, dan putih 100%. Faktor intensitas teridiri dari atas 2 taraf yaitu intensitas 3 strip dan intensitas 5 strip. Penelitian dilakukan dengan 3 ulangan yang masing-masing terdapat 6 satuan percobaan. Berdasarkan hasil penelitian diketahui bahwa LED spektrum biru 5 strip dan kombinasi merah 25% biru 75% 3 strip dapat mempercepat pertumbuhan pucuk tanaman stroberi. Spektrum kombinasi cahaya merah 25% biru 75% dengan intensitas 5 strip lebih baik dalam mengakumulasikan klorofil dibanding perlakuan lainnya. Spektrum dan intensitas LED tidak memberikan pengaruh yang signifikan terhadap kandungan glukosa daun. Penggunaan cahaya putih 5 strip dapat meningkatkan akumulasi fruktosa daun. Penelitian metabolit sekunder daun menunjukkan ada 4 senyawa umum yang ada pada semua perlakuan. Setiap kombinasi perlakuan spektrum dan intensitas memiliki minimal 1 senyawa spesifik hasil metabolit sekunder kecuali perlakuan merah 50% biru 50% 3 strip., Strawberry (Fragaria x ananassa Duch.) is a plant originated from subtropical areas and can be cultivated in tropical areas. Efforts to adapt the growing environment from plant origin can be done by using LEDs as artificial light. This study aims to determine the physiological response of strawberry plants given LED treatment. The study used a complete randomized block design (RBD) which consisted of 2 treatments, namely light spectrum and intensity. Spectrum factor consists of 6 levels, 100% red, 100% blue, 75% red 25% blue, 25% red 25% blue 75%, 50% red 50% blue, and 100% white. The intensity factor consists of 2 levels, 3 strips and 5 strips. The research was conducted with 6 sample and 3 replicate. The study shows the use of 5 strips blue spectrum LED and 3 strips combination of 25% red 75% blue LED can accelerate shoot growth in strawberry. The combination of 5 strips 25% red 75% blue LED treatment is better on accumulate chlorophyll then other treatment. The spectrum and intensity of the LED did not give a significant effect on leaf glucose levels. The use of 5 strips white LED can increase the amount of fructose in the leaves. Research on profiling secondary metabolites shows that strawberry leaves produced 4 common compounds present in all treatments. Each combination of spectrum and intensity treatment has at least 1 specific compound resulting from secondary metabolites except the 50% red 50% blue 3 strips treatment.
Keyword: buah subtropis, cahaya, gula, klorofil, kromatografi, chlorophyll, chromatography, lights, subtropical fruits, sugar content