Judul: The Effectiveness of Indole-3-Butyric Acid (IBA) on the Growth of Mutant Roots of Alfalfa (Medicago sativa L.) Acid-Resistant pH 3.6 in In Vitro Culture
Abstrak: Alfalfa (Medicago sativa L.) is a type of legume forage that has the potential to be used as a source of animal feed. Alfalfa has a high protein content and is good as animal feed. Alfalfa plant mutants resistant to pH 3.6 have been produced, a collection of Pastura Laboratory, Department of Nutrition and Feed Technology, Faculty of Animal Husbandry, IPB University with limited seed production capabilities. One of the efforts to cultivate and multiply the culture of alfalfa plant collections that are pH 3.6 resistant is by vegetative propagation through tissue culture. Tissue culture is a plant cultivation technique that is carried out in vitro. The tissue culture technique has several stages in the process, namely explant culture initiation, propagation, rooting and plant acclimatization. Root formation can be stimulated more rapidly by auxin as a growth regulator. The purpose of this study was to determine the effectiveness of IBA on root growth of alfalfa (Medicago sativa L.) mutant acid resistance pH 3.6 through tissue culture techniques. The method used in this study was a completely randomized design (CRD) with 5 treatments, namely control, IBA 0.5 ppm, 1.0 ppm, 1.5 ppm and 2.0 ppm. The data obtained were analyzed using Analysis of Variance (ANOVA) with SPSS version 16.0 and the difference in significance between treatments was further analyzed using Tukey's test. The variables observed were root length, number of rooted plants, plant height, number of leaves and shoots, leaf fall, plant mortality, plant biomass and media loss. The results showed that the number of rooted plants and the increase in root length of alfalfa plants gave a good response to 2.0 ppm. The conclusion of this study is the effective level of adding IBA to mutant plants Alfalfa (Medicago sativa L.) acid resistant pH 3.6 is 2.0 ppm. Increased level of compassion also had a positive correlation with root growth, leaf formation and vertical height gain but had a negative correlation with shoot formation, biomass and media shrinkage., Alfalfa (Medicago sativa L.) merupakan salah satu hijauan jenis leguminosa yang berpotensi untuk dimanfaatkan sebagai sumber pakan ternak. Alfalfa memiliki kandungan protein yang tinggi dan baik sebagai pakan ternak. Telah dihasilkan mutan tanaman alfalfa tahan pH 3.6 koleksi Laboratorium Pastura, Departemen Ilmu Nutrisi dan Teknologi Pakan, Fakultas Peternakan, Institut Pertanian Bogor dengan kemampuan produksi biji yang terbatas. Salah satu upaya pembudidayaan dan perbanyakan kultur koleksi tanaman alfalfa tahan pH 3.6 adalah dengan perbanyakan vegetatif melalui kultur jaringan. Kultur jaringan merupakan teknik budidaya tanaman yang dilakukan secara in vitro. Teknik kultur jaringan memiliki beberapa tahapan dalam prosesnya, yaitu inisiasi kultur eksplan, perbanyakan, perakaran dan aklimatisasi tanaman. Pembentukan akar dapat dirangsang lebih cepat oleh auksin sebagai pengatur tumbuh. Tujuan penelitian ini adalah untuk mengukur efektifitas IBA terhadap pertumbuhan akar mutan tanaman alfalfa (Medicago sativa L.) tahan asam pH 3.6 melalui teknik kultur jaringan. Metode yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) dengan 5 perlakuan yaitu Kontrol, IBA 0.5 ppm, 1.0 ppm, 1.5 ppm dan 2.0 ppm. Data yang diperoleh dianalisis menggunakan Analysis of Variance (ANOVA) dengan program SPSS versi 16.0 dan perbedaan signifikansi
Keyword: Alfalfa (Medicago Sativa L.), IBA, Issues culture