Judul: Analysis of the Effect of Bacillus sp. Probiotic Application for Controlling Vibrio spp. Bacterial Population in Vannamei Shrimp (Litopenaeus vannamei) Cultivation.
Abstrak: Bakteri Vibrio spp. merupakan salah satu jenis bakteri patogen yang sering ditemukan di lingkungan perairan, seperti pada budidaya udang. Infeksi Vibrio spp. mampu menyebabkan beberapa penyakit pada udang seperti vibriosis, Acute Hepatopancreatic Necrosis Disease (AHPND), dan feses putih. Pengendalian jumlah populasi dan infeksi oleh Vibrio spp. dalam budidaya udang dengan penggunaan probiotik Bacillus sp. pada pakan udang. Bakteri Bacillus sp. memiliki kemampuan anti quorum sensing dengan mengganggu acyl homoserine lactones (AHL), sehingga AHL Vibrio spp. tidak mencapai kondisi kuorum. Penelitian ini bertujuan menganalisis pengaruh aplikasi probiotik Bacillus sp. untuk pengendalian populasi bakteri Vibrio spp. pada budidaya udang vaname. Penelitian dilakukan hingga Day of Culture (DOC) 120 pada 6 kolam tambak terletak di PT. Sentra Budidaya Biotek, Kabupaten Sumbawa Barat, Nusa Tenggara Barat. Metode yang digunakan terdiri dari persiapan tambak, aklimatisasi benur, perbanyakan dan aplikasi probiotik Bacillus sp., Pemeriksaan kualitas air, isolasi dan perhitungan bakteri Vibrio spp., uji histopatologi hepatopankreas udang, sampling udang, dan panen udang. Aplikasi probiotik Bacillus sp. memberikan dampak positif terhadap pengendalian populasi bakteri Vibrio spp., kualitas air tambak, uji histopatologi hepatopankreas udang, dan hasil panen udang. Hal ini ditunjukkan dengan populasi Vibrio spp. dibawah ambang batas, terkendalinya kualitas air, terkendalinya infeksi dari Vibrio spp. pada hepatopankreas udang dan jumlah produksi udang mencapai target., Vibrio spp. are a type of pathogenic bacteria commonly found in aquatic environments, such as in shrimp pond. Infections by Vibrio spp. can lead to several shrimp diseases such as vibriosis, Acute Hepatopancreatic Necrosis Disease (AHPND), and white feces syndrome. Controlling the population and infections of Vibrio spp. in shrimp pond can be achieved through the use of Bacillus sp. in shrimp feed. Bacillus sp. have anti-quorum sensing abilities by disrupting acyl homoserine lactones (AHL), preventing Vibrio spp. from reaching quorum conditions. This study aims to analyze the effects of Bacillus sp. controlling the population of Vibrio spp. in vannamei shrimp pond. The research was conducted until Day of Culture (DOC) 120 in 6 ponds located at PT. Sentra Budidaya Biotek, West Sumbawa, West Nusa Tenggara. The methods included pond preparation, shrimp acclimatization, Bacillus sp. culture and application, water quality assessment, isolation and quantification of Vibrio spp., histopathological examination of hepatopancreas, shrimp sampling, and harvest. The application of Bacillus sp. showed positive impacts in suppressing Vibrio spp. populations, maintaining pond water quality, reducing Vibrio spp. infections in shrimp hepatopancreas, and shrimp production. This was evidenced by Vibrio spp. populations staying below threshold limits, controlled water quality, controlled infections from Vibrio spp. in shrimp hepatopancreas, and achieving the targeted shrimp production.
Keyword: Aplikasi, Litopenaeus vannamei, anti-quorum sensing, Bacillus  sp., Vibrio spp.