arulpm/triplet_large huggingface dataset:
Judul: IPB (Bogor Agricultural University)
Abstrak: Trehalosa adalah disakarida yang telah digunakan sebagai krioprotektan ekstraseluler dalam kriopreservasi sel. Trehalosa melindungi viabilitas sel dan menstabilisasi protein dalam kondisi lingkungan yang ekstrem. Penggunaan trehalosa sebagai krioprotektan intraseluler berpotensi menggantikan dimetil sulfoksida yang bersifat sitotoksik namun sangat efektif sebagai kriomedium intrasel. Penelitian sebelumnya mendapati bahwa pemuatan trehalosa difasilitasi oleh endositosis yang bergantung pada mikrotubulus namun tidak dipengaruhi kinerja aktin. Penentuan tipe endositosis yang memfasilitasi pemuatan trehalosa membutuhkan evaluasi lebih lanjut. Penelitian ini bertujuan memberikan bukti langsung tipe endositosis yang memfasilitasi pemuatan trehalosa pada sel manusia, khususnya pada sel fibroblas. Penelitian ini fokus pada penggunaan tiga inhibitor endositosis spesifik untuk membedakan endositosis berbasis clathrin dan yang tidak berbasis clathrin, yaitu pitstop-2, dynasore, dan cytochalasin D. Penelitian ini juga memberikan gambaran viabilitas sel progenitor mesenkim terhadap paparan trehalosa, bukti pemuatan trehalosa ke dalam sitoplasma fibroblast, dan upaya mengaplikasikan trehalosa sebagai krioprotektan intraseluler dan ekstraseluler dalam melindungi viabilitas dan fungsi perlekatan pada proses kriopreservasi sel fibroblas. Hasil penelitian menunjukkan bahwa paparan trehalosa aman terhadap sel progenitor mesenkim meski memiliki sifat sebagai osmolit, trehalosa dapat masuk ke dalam sitoplasma sel fibroblas dengan mekanisme pemuatan trehalosa yang dihambat secara signfikan oleh pitstop-2, dynasore, dan cytochalasin D (p<0.05). Hasil ini menjadi bukti langsung bahwa pemuatan trehalosa difasilitasi oleh endositosis berbasis clathrin. Meski demikian, tidak ada eksperimen yang berhasil sepenuhnya menghambat pemuatan trehalosa walaupun digunakan kombinasi inhibitor pitstop-2 dan dynasore sehingga diperkirakan mekanisme endositosis non-spesifik lain turut terlibat dalam pemuatan trehalosa.
Keyword: Trehalosa, Endositosis, Fibroblast, Kriopreservasi, Clathrin
Judul: In Silico Screening of COVID-19 Drug Candidate Compounds against SARS-CoV-2 Spike Proteins through ACE2 and TMPRSS2 Approaches
Abstrak: SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2) menginfeksi manusia melalui ikatan spike protein virus dengan dua reseptor pada sel inang, yaitu ACE2 (Angiotensin Converting enzyme 2) dan TMPRSS2 (Transmembrane Serine Protease 2). Penelitian ini bertujuan menemukan senyawa kandidat obat COVID-19 (Coronavirus disease) berdasarkan perubahan energi bebas Gibbs dengan pendekatan simulasi molekuler. Penambatan senyawa kandidat dilakukan terhadap tiga tapak aktif pada spike protein virus, yaitu tapak aktif yang berikatan dengan ACE2, bagian satu TMPRSS2, dan bagian dua TMPRSS2. Penambatan 115 ligan dikelompokkan menjadi lima kelompok, yaitu kelompok fitokimia, kelompok minyak atsiri, kelompok penghambat protease, kelompok organisme laut, dan kelompok obat sintesis dari hasil peneltian Elvira (2020). Hasil penambatan senyawa kandidat pada tapak aktif dengan ACE2 didapatkan senyawa asparagosida-F dengan nilai ΔG terendah, yaitu -9,8 kkal/mol. Hasil penambatan senyawa kandidat pada tapak aktif dengan bagian satu TMPRSS2 didapatkan senyawa shatavarin-X dengan nilai ΔG terendah, yaitu -13,3 kkal/mol. Hasil penambatan senyawa kandidat pada tapak aktif dengan bagian dua TMPRSS2 didapatkan senyawa shatavarosida-B dengan nilai ΔG terendah, yaitu -9,1 kkal/mol. Hal tersebut menunjukan penambatan oleh ketiga senyawa kandidat akan berjalan spontan. Namun, hasil analisis toksistas Lipinski dan Toxtree menunjukkan bahwa ketiga senyawa tersebut tidak dapat diberikan secara oral.
Keyword: ACE2, drug, molecular docking, spike protein, TMPRSS
Judul: Keragaman Gen TP53INP1 dan Asosiasinya dengan Komposisi Asam Lemak Daging Domba
Abstrak: Gen TP53INP1 adalah salah satu gen yang terlibat dalam pengaturan metabolisme lemak dan memiliki keterkaitan dengan komposisi asam lemak pada domba. Penelitian ini bertujuan mengindentifikasi keragaman gen TP53INP1 dan asosiasinya dengan komposisi asam lemak daging domba lokal Indonesia. Total sampel yang digunakan sebanyak 130 sampel DNA ekor domba jantan yang terdiri dari 10 ekor domba barbados cross (DBC), 10 ekor domba compass agrinak (DCA), 20 ekor domba ekor gemuk (DEG), 53 ekor domba ekor tipis (DET), 10 ekor domba komposit garut (DKG), dan 27 ekor domba jonggol (DJG). Identifikasi keragaman gen TP53INP1|NlAIII menggunakan metode Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Ukuran produk PCR yang teramplifikasi memiliki panjang basa sebesar 457 bp. Keragaman gen TP53INP1 ditemukan pada semua jenis sampel domba dengan genotipe AA, AG, dan GG. Frekuensi alel TP53INP1|NlAIII berada dalam keseimbangan Hardy-Weinberg kecuali pada domba ekor tipis (DET). Asosisiasi SNP gen TP53INP1 dengan asam lemak menggunakan uji T (P<0,05) pada domba yaitu asam palmitat (C16:0), asam behenat (C22:0), asam lignoserat (C24:0), asam palmitoleat (C16:1), asam elaidat (C18:1n9t), asam gondoat (C20:1), asam nervonat (C24:1), asam oleat (C18:1n9c), dan asam linolelaidat (C18:2n9t). Disimpulkan bahwa SNP gen TP53INP1 mempengaruhi komposisi asam lemak domba., TP53INP1 gene is one of the genes involved in regulating lipid metabolism and is related to the fatty acid composition of sheep. This study aims to identify the diversity of TP53INP1 gene and it’s association with the fatty acid composition of Indonesian local sheeps. The total sample used was 130 ram DNA samples consisting of 10 barbados cross (DBC), 10 agrinak compass sheep (DCA), 20 javanese fat tailed (DEG), 53 javanese thin tailed (DET), 10 garut composite sheep (DKG), and 27 jonggol sheep (DJG). Identification of the diversity of TP53INP1|NlAIII was identified by the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. The amplified PCR product had 457 bp. The diversity of the TP53INP1 gene was found in all types of sheep samples with the genotypes AA, AG, and GG. The allele frequency was in Hardy-Weinberg equilibrium except for the thin tailed sheep (DET). SNP TP53INP1 gene used the T test was associated (P<0,05) with fatty acids in sheeps, such as palmitic acid (C16:0), behenic acid (C22:0), lignoceric acid (C24:0), palmitoleic acid (C16:1), elaidic acid ( C18:1n9t), gondoic acid (C20:1), nervonic acid (C24:1), oleic acid (C18:1n9c), and linolelaidic acid (C18:2n9t). It could be concluded that the SNP TP53INP1 gene effects to the fatty acid composition of lamb.
Keyword: TP53INP1 gene, fatty acids, PCR-RFLP, sheep
Judul: Identifikasi Karakter Morfologi dan Molekuler Toleransi Kekeringan pada Tanaman Kakao (Theobroma cacao L.).
Abstrak: Cekaman kekeringan merupakan salah satu masalah dalam pertanaman maupun produksi kakao. Cekaman kekeringan dapat mengakibatkan perubahan tanaman baik dari morfologi, fisiologi, biokimia, dan molekuler. Kekeringan akan berdampak bagi pertumbuhan dan perkembangan tanaman serta ketidakseimbangan metabolisme oksidatif. Dampak cekaman kekeringan pada tanaman kakao dapat diatasi dengan menggunakan klon unggul atau hibrida unggul yang memiliki toreransi terhadap cekaman kekeringan. Studi pewarisan terhadap toleransi cekaman kekeringan pada tanaman kakao masih terbatas. Penelitian mengenai studi pewarisan toleransi terhadap cekaman kekeringan pada tanaman kakao perlu dilakukan untuk mendapatkan genotipe hibrida kakao toleran cekaman kekeringan. Penelitian ini juga dilakukan untuk mengetahui karakter morfologi yang dapat digunakan untuk seleksi. Pendekatan molekuler dengan gen APX digunakan untuk mengkonfirmasi karakter toleransi terhadap cekaman kekeringan pada tanaman kakao. Berdasarkan hasil penelitian, tanaman pada kondisi tercekam menunjukkan pertumbuhan dan perkembangan yang lebih lambat dibandingkan dengan tanaman dalam kondisi normal. Karakter yang berkaitan dengan sistem perakaran berkaitan dengan respon tanaman terhadap cekaman kekeringan. Berdasarkan hasil analisis ragam menggunakan desai persilangan NC II (North Carolina II), karakter terkait cekaman kekeringan dikendalikan oleh aksi gen aditif. Pola dominan berpengaruh terhadap karakter bobot basah akar dan rasio akar/tajuk. Genotipe persilangan Sulawesi 3 x ICCRI 09 menunjukkan nilai DGK (daya gabung khusus) terbaik pada karakter diameter batang, bobot basah akar, dan bobot kering tajuk. Hasil analisis sekuen DNA gen APX, gen APX teramati merupakan gen APX sitosolik. Dengan basa nukleotida yang dapat membedakan tetua toleran T (timin) dan rentan A (adenin). Asam amino yang dihasilkan pada tetua toleran F (phenylalanine) dan pada tetua rentan I (isoleucine).
Keyword: ekaman kekeringan, DGK, NC II, pola pewarisan, DGU, gen APX
Judul: Evaluasi struktur perpustakaan agrotropika Institut Pertanian Bogor
Abstrak: Analysis and structure evaluation is necessary in order to achieve a better result, because a successful construction depends on good planning and execution at site will take the rest. Calculation must refer to prevailed regulation. This script is going to evaluate and analyse Agrotropica library of Bogor Agricultural University. The library has been built precisely next to LSI. The building which was designed by PT Fajar Adhi Karya has four main floor, basement and lift machine room control. The method of this research was data collection through As Built Drawing, building modelling using SAP 2000 version 14, define frame section of structure, define and assign load case to it’s model, design the reinforcement of concrete from SAP 2000 version 14’s output such as internal force and compare the result of measurement to existing condition. This research refer to SNI 03 2847 2002 which contained concrete reinforcement design regulation. Structure evaluation input data must have according to As Built Drawing such as dimension, reinforcement type, material type, in order to get effective amount of reinforcement. Stress reinforcement on beam calculation has the biggest differences getting to 166%, and shear reinforcement smallest space 89 mm that hard to execute at the site. Column stress reinforcement evaluation has to be checked towards X and Y direction, all of column in this building was safe except K4 column type toward y direction and according to calculation that ultimate shear force didn’t exceeded nominal shear force, in other word all of column shear reinforcement type in this building is safe. Slab reinforcement design has been planned by moment coefficient method with two direction. It can be seen that N, O, P slab type has larger space than calculation, it need maximum space 120 mm on x direction and 130 mm on y direction but it has been set by M8 – 150 wiremesh which mean 150 mm x direction and 150 mm y direction at site. Pilecaps calculation result shows that it’s need shear reinforcement because ultimate shear force has exceeded nominal shear force. Amount of Stress reinforcement on P4, P8, P9 pilecaps type at site less than calculation because ultimate moment was too big. There are some differencess between calculation and existing condition that may caused by input load data to its modeling and regulation which used by designer.
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Judul: Ikat Silang Pati Sagu Dengan Gluten Untuk Meningkatkan Daya Mengembang Sagu Sebagai Bahan Utama Adonan
Abstrak: Pengikatan silang antara pati sagu dan gluten dilakukan dalam upaya meningkatkan pemanfaatan sagu yang potensinya sangat besar di Indonesia. Upaya pengikatan silang antara keduanya dilakukan untuk menghasilkan sagu dengan daya mengembang yang lebih baik. Penelitian ini bertujuan menghasilkan tepung sagu yang terikatsilang dengan gluten serta mengetahui pengaruh penambahan gluten dan suhu proses ikat silang. Penelitian dilakukan dengan mencampur pati sagu dan gluten dengan penambahan gluten 10%, 30%, dan 50% dari bobot sagu, baik dengan reaksi pemanasan suhu 50 dan 65 ˚C maupun tanpa reaksi. Hasil penelitian menunjukkan tepung yang di buat dengan proses ikat silang pada 50 ˚C dan penambahan gluten 30% (CL30P50C) memiliki swelling power dan baking expansion terbesar yaitu 61 ± 2% dan 1.03 ml/g ± 2% dengan derajat pengembangan sebesar 21% dari sagu murni dan kadar protein 9.44%. Daya serap tepung terhadap air semakin meningkat dengan dilakukannya reaksi pemanasan dan seiring ditingkatkannya penambahan gluten. Analisis FTIR menunjukkan terjadinya ikatan silang antara pati sagu dan gluten pada produk CL30P50C dengan menguatnya puncak spektrum inframerah ikatan C-N pada bilangan gelombang 1158.8 cm-1.
Keyword: baking expansion, ikat silang, gluten, pati sagu, swelling power
Judul: Karakteristik Dan Analisis Keuntungan  Usahaternak Sapi Perah Dki Jakarta  (Studi Kasus Di Wilayah Kelurahan Pondok  Ranggon, Jakarta Timur)
Abstrak: Usahaternak sapi perah masih banyak ditemukan di wilayah DKI Jakarta. Penelitian ini bertujuan untuk mengetahui karakteristik dan keuntungan usahaternak sapi perah di kelurahan Pondok Rangon, Kecamatan Cipayung, Jakarta Timur. Responden pada penelitian ini sebanyak 20 peternak. Pada studi ini dipelajari mengenai karakteristik peternak, manajemen peternakan dan manajemen kesehatan ternak. Peternak dikelompokan menjadi dua, yaitu kelompok pertama adalah peternak dengan kepemilikan ternak kurang dari 43 ekor dan kelompok kedua adalah peternak dengan kepemilikan ternak lebih dari dan sama dengan 43 ekor. Analisis keuntungan dilakukan dengan menghitung selisih antara input dan output. Berdasarkan hasil perhitungan, diketahui keuntungan per bulan pada peternak kelompok pertama sebesar Rp5.815.121,00 dan pada peternak kelompok kedua sebesar Rp21.861.559,00. Studi ini menunjukkan bahwa usaha ternak sapi perah di wilayah Pondok Ranggon DKI Jakarta masih menguntungkan. Kata kunci: usahaternak, sapi perah, keuntungan
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Judul: The effects of biofertilizer with different drying system and storage period on growth and production of tomato and potato in the field
Abstrak: Biofertilizer is one alternative of fertilizer that is able to increase soil fertility and crop production. The purpose of this research was to investigate the effect of biofertilizer dried by different methods and exposed to different period of storage on nutrient uptake, vegetative growth and production of potato and tomato plant grown in the field. Two experiments were carried out in the field Laboratory of Baltsa Lembang, Bandung. The first experiment was prepared using Randomized Block Design with one factor (biofertilizer application) including i.e (B0) without biofertlizer (control), (B1) liquid biofertilizer, (B2) freezedried biofertilizer without storage, (B3) freezedried biofertilizer with 3 months storage, (B4) centrifuged biofertilizer without storage and (B5) centrifuged biofertilizer with 3 months storage. In this experiment 3 replications (blocks) were applied with total of 18 combination blocks. The second experiment was also prepared using Randomized Block Design with two factors. The first factor was anorganic NPK dosage by (A1) 50% NPK and (A2) 100% NPK and the second factor was biofertilizer application i.e (B0) without biofertlizer (control), (B1) liquid biofertilizer, (B2) freezedried biofertilizer without storage, (B3) freezedried biofertilizer with 3 months storage for tomato, whereas for potato, the factors of (B2) freezedried biofertilizer, (B3) centrifuged biofertilizer were applied. Besides that the plant were fertilized with manuare by about 20 ton/Ha. In this experiment 3 replications (blocks) were applied with total of 24 combination blocks. The results showed that viability of bacterium tended to decline during storage of biofertilizer. The storage of biofertilizer during 3 months did not significantly reduce the effect on growth and production of plant. Application of biofertilizer increased macro and micro nutrient absorption, vegetative growth and plant production. In tomato plant application of biofertilizer increase total macro and micro nutrient as well as plant growth parameters. Plant production also increased as response to biofertilizer by about 112.1% that was indicated by application of B4 for fruit weight per plant. Application of biofertilizer in combination with 100% NPK was almost similar to that of 50% NPK in improving growth and production of plant. All application of biofertilizer significantly reduced disease attack caused by alternaria solani fungus. In potato plant, macro and micro nutrient absorption and root dry weight increased significantly by application of biofertilizer but not on shoot parameters. Plant production, also increased as response to biofertilizer by about 41.6 % indicated by application of centrifuged biofertilizer (B4) for tuber weight per plant and increase plant production per plot by about 79.5 % that was indicated by application of 50 % NPK+ centrifuged biofertilizer (A1B3).
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Judul: Aplikasi Gelatin Tipe A Dari Kulit Sapi Dalam Pembuatan Krim Tabir Surya (Sunscreen Cream)
Abstrak: The utilization of slaughterhouse by product is by producing gelatin from splitting hide. Gelatin has numerous features as gelling, thickening, water binding, emulsifying, foaming and stabilizing, so it can be applied to many industries, including cosmetic. The purpose of this research is to determine the gelatin's concentration in making sunscreen, to find the best active material in making sunscreen, and to get information about the characteristic of sunscreen, which use gelatin as emulsifier and thickener. In this research, we use gelatin type A. Generally; the production of sunscreen is by mixing the oil soluble component with water soluble component. Oil soluble component are cremophor A-6, cremophor A-25, isopropyl palmitate, cetyl alcohol, myritol and active material; while water soluble component are aquadest, gelatin, propylene glycol and allantoin. After both components are mixed, then preservative as anti microorganism can be added when the temperature of the product below 40°C. Analysis taken to the sunscreen cream are homogenity, color, sticky condition, cool condition on skin, easiness in spreading on the skin, pH, density, viscosity and Sun Protective Factor (SPF). From the research we conclude that gelatin's concentration and active material influence color, viscosity, cool condition on skin, sticky condition on skin and homogenity. Sun Protective Factor (SPF) and pH (acidity) are significantly influenced by active material factor, while cool condition on the skin isn't influenced by the above factors. At last, from this research it can be concluded that the best gelatin's concentration is 3% and the active material is titanium dioxide.
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Judul: Jaringan Komunikasi Dalam Penerapan Teknologi Produksi Ubi Kayu (Kasus Petani Ubi Kayu di Desa Suko Binangun, Kecamatan Way Seputih, Kabupaten Lampung Tengah, Provinsi Lampung)
Abstrak: In order to increase cassava production, farmers need an adequate and trusted information to gain their purpose. Fulfilling their information requirement of cassava production technology, farmers establish a communication network among farmers. The objectives of this research were: (1) to describe communication network among farmers (2) to analyze the relationship between personal characteristics of farmer and the communication network (3) to analyze the relationship between communication network and the implementation of cassava production technology. The unit of analysis were cassavas farmer. A hundred farmers were taken as sample by using sampling intact system.This research resulted several outputs i.e : (1) communication network about seeds, fertilizer, pets and diseases were radial personal network and communication network about harvest was interlocking personal network (2) there was a significant relationship between income, group involvement, mass media ownership, arable land area with local centrality. There was also a significant correlation between educational level, revenue, group involvement, mass media ownership with global centrality. (3) there was a significant relationship between local centrality, global centrality and the implementation of cassavas production technology.
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Judul: Perbandingan Distribusi Otot Kambing Kacang dan Peranakan Etawah pada Bobot Lepas
Abstrak: A total of 43 goats which consist of 27 kacang goats and 16 peranakan etawah goatswas used to evaluate the comparison of muscle distribution between kacang goat and PE goat slaughtered over the weight 10 Kg. The data of body components was analyzed using Analysis of Variance (ANOVA) while muscle of distribution and other body components were analyzed using Analysis of Covariance (ANCOVA) with slaughter weight, half carcass weight as a covariable. The result showed that groups of muscles and body components among these breeds were no significantly different (P>0.05). There were no significantly differences among breeds in muscles of groups except the percentages of muscles of groups 3 and the muscles of groups 5. There were no significantly differences among breeds in body components except inter muscle fat weight, subcutan fat weight, inter muscle fat percentage, subcutan fat percentage, and kidney fat percentage. In general, the major muscles groups percentage (groups 1+3+5) in kacang goat is 60.35% and 60.71% for peranakan etawah goat.
Keyword: body components, muscles distribution, goat, breads