Judul: Aklimatisasi Rumput Laut Kappaphycus alvarezii Hasil Kultur Jaringan dengan Kepadatan yang Berbeda dalam Akuarium di Rumah Kaca
Abstrak: Seaweed is one leading commodity of Indonesian, which has a large market, both domestically and exports. The successful cultivation of seaweed must be support by the use of supreme seeds seaweed. Availability of supreme seeds can be helped with breeding techniques through tissue culture. Seeds produce by tissue culture accustomed alive with sterile sea water in the bottle, beside that the seeds is not strong enough to live at sea directly, and still has a small size, so it needs to be done acclimatization. Acclimatization is the process of adaptation to the new environment. The stages of Acclimatization includes: 1) How to keep biota alive, 2) How can the biota growth, 3) How the biota can breeding. This research aimed to examine the survival and growth, as well as getting the optimum density Kappaphycus alvarezii during acclimatization in the aquarium. The seaweed placed at the base of the aquarium with early biomass density of, 95, 143, 191, dan 238 g/m33. The number of individuals were used are 33-137 individuals. Seaweed has variation in individual size and number of the talus. The length of the seeds were used are 1.6-2.2 cm and weights 0.15-0.32 g. The seaweed were cultivation in the aquarium of 90x30x35 cm filted seawater 81 L with water recirculation systems and water exchange of 50% for 2 weeks once. The parameters were measured during the study i.e., survival rate, specific growth rate, biomass and individual weights, base and middle diameter growth of thallus, length growth and total length of the thallus, the total absorption of phosphate, nitrate, orthophosphate, nitrite, and ammonia in the water. The nitrogen and phosphorus absorption by seaweed thallus were analyzed by with proximate analysis. Results of the study showed that from seaweed tissue culture at the level of different early density biomass are able to adaptation in the aquarium system and survive well with rate reaches 100%. The specific growth rate, individual specific growth rate and base diameter growth of seaweed (y) was decreases parallel with increasing initial biomass density (x) of seaweed followed consecutive equations y = 4.600–0.2801x; y = 3.929–0.1661x; dan y = 0.7883–0.1317x (P<0.2). The growth of seaweed biomass (y) was increasing up to the initial density biomass (x) of seaweed 191 g/m3, and then decline in initial density biomass (x) of seaweed 238 g/m3 follows the y = 23.73+54.99x–8.440x2 (P<0.2). Based on the highest level of nitrogen and phosphorus contens in seaweed, the optimum density is 95 g/m3, while based on the space efficiency and sea water medium, the optimum density is 143 and 191 g/m3., Rumput laut merupakan salah satu komoditas unggulan perikanan di Indonesia yang memiliki pangsa besar, baik di dalam negeri maupun luar negeri. Keberhasilan budidaya rumput laut salah satunya dipengaruhi oleh pengunaan bibit rumput laut yang unggul. Ketersediaan bibit unggul dapat dibantu dengan teknik pengembangbiakan melalui kultur jaringan. Bibit hasil kultur jaringan terbiasa hidup dengan air laut steril di dalam botol, selain itu masih belum kuat jika harus dipelihara langsung di laut, dan masih memiliki ukuran yang kecil, sehingga perlu dilakukan aklimatisasi. Aklimatisasi merupakan suatu proses adaptasi terhadap
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