Judul: Effects of Megasphaera elsdenii Probiotic Supplementation on the Fermentability and Concentration of Rumen Lactic Acid in Preventing Acidosis
Abstrak: Megasphaera elsdenii adalah spesies pengguna laktat yang menonjol dalam rumen. Penggunan M. elsdenii sebagai probiotik karena kemampuannya dalam memanfaatkan laktat, sehingga menurunkan akumulasi laktat dalam rumen dan menstabilkan pH. Penelitian ini dilakukan untuk mengevaluasi penggunaan M. elsdenii secara in vivo terhadap fermentasi rumen dan konsentrasi asam laktat dalam mencegah asidosis pada ternak, dengan mengintegrasikan data dari berbagai studi terkait menggunakan model matematis analisis regresi. Sebanyak 15 studi diperoleh 66 data. Parameter meliputi konsentrasi asam laktat, produk fermentasi, dan populasi mikroba rumen. Data dianalisis menggunakan analisis regresi dengan software IBM SPSS versi 20. Hasil analisis menunjukkan bahwa suplementasi probiotik M. elsdenii memberikan efek penurunan signifikan (P<0.05) terhadap konsentrasi asam laktat, proporsi asetat, rasio asetat:propionat, dan cenderung signifikan terhadap penurunan metan (P<0.1). Selain itu, suplementasi M. elsdenii juga berpengaruh sangat signifikan (P<0.01) terhadap peningkatan total VFA dan proporsi butirat, serta berpengaruh secara signifikan (P<0.05) terhadap peningkatan proporsi propionat, proporsi isobutirat, dan cenderung signifikan (P<0.1) terhadap peningatan proporsi valerat. Namun pemberian probiotik M. elsdenii ini tidak berpengaruh signifikan (P>0.10) terhadap pH, NH3, proporsi isovalerat, dan populasi protozoa di dalam rumen. Dapat disimpulkan bahwa suplementasi probiotik M. elsdenii secara in vivo terbukti mampu menurunkan konsentrasi asam laktat dan meningkatkan beberapa produk hasil fermentasi rumen., Megasphaera elsdenii is a lactate utilizing species that predominates in the rumen. The use of M. elsdenii as a probiotic is due to its ability to utilize lactate, thereby reducing the accumulation of lactate in the rumen and stabilizing pH. This study was conducted to evaluate the use of M. elsdenii in vivo to rumen fermentation and lactic acid concentration in preventing acidosis in livestock, by integrating data from various related studies using a regression analysis mathematical model. A total of 15 studies were obtained that considered of 66 data points. The parameters integrated were lactic acid concentration, fermentation products, and rumen microbial populations. The data were subsequently analyzed by using a regression analysis and employed IBM SPSS version 20 statistical software. Results revealed that probiotic supplementation of M. elsdenii had a significant reduction effect (P <0.05) on the concentration of lactic acid, proportion of acetate, ratio of acetate: propionate and tended to be significant (P <0.1) on an decrease of methane production. In addition, M. elsdenii supplementation also had a very significant effect (P <0.01) on the increase in total VFA and butyrate proportion, and had a significant effect (P <0.05) on the increase in propionate proportion, isobutyrate proportion, and tended to be significant (P <0.1) on an increase in the proportion of valerate. However, giving probiotic M. elsdenii did not have a significant effect (P> 0.10) on pH, NH3, proportion of isovalerate, and protozoa population in the rumen. It can be concluded that the effect of probiotic M. elsdenii supplementation in vivo is proven to be able to reduce lactic acid concentrations and increase some rumen fermentation products.
Keyword: acidosis, lactic acid, Megasphaera elsdenii, regression analysis