Judul: Conversion of Borate-Based Bioglass to Hydroxyapatite in Phosphate Solution and Mixed of Phosphate and Carbonate Solution
Abstrak: Bioaktivitas material dengan sel merupakan salah satu parameter utama biokompatibilitas material yang berperan dalam regenerasi jaringan tulang baru. Biomaterial dengan luas permukaan yang besar mampu meningkatkan adhesi sel yang berperan dalam inisiasi regenerasi jaringan baru. Salah satu modifikasi untuk meningkatkan bioaktivitas material adalah pembuatan material dalam bentuk mikrosfer. Dalam penelitian ini dilakukan fabrikasi mikrosfer hidroksiapatit melalui konversi mikrosfer bioglass dalam larutan fosfat serta larutan campuran fosfat dan karbonat. Mikrosfer bioglass diperoleh melalui metode flame spray spheroidization dengan bahan baku dasar partikel bioglass berukuran 63 μm – 200 μm. Citra SEM menunjukkan bahwa mikrosfer bioglass berhasil difabrikasi dengan ukuran rata-rata 76,84 μm. Konversi mikrosfer bioglass dilakukan dengan variasi waktu pengamatan selama 14 hari. Hasil pola difraksi sinar-X menunjukkan bahwa mikrosfer kalsium fosfat untuk kedua larutan terbentuk pada hari ke-7 dan terdapat fasa apatit karbonat (CHA) pada hasil uji konversi dalam larutan campuran fosfat dan karbonat. Citra SEM pada hasil konversi menunjukkan bahwa ukuran mikrosfer kalsium fosfat memiliki rentang yang sama dengan ukuran mikrosfer bioglass sebelum uji konversi yaitu sebesar 73,59 μm – 90,83 μm. Hasil EDX menginformasikan bahwa mikrosfer kalsium fosfat memiliki rasio Ca/P yang mendekati literatur., The bioactivity of the material with cells is one of the main parameters for the biocompatibility of the material that plays a role in the regeneration of new bone tissue. Biomaterials with a large surface area are able to increase cell adhesion which plays a role in the initiation of new tissue regeneration. One of the modifications to increase the bioactivity of the material is the manufacture of the material in the form of microspheres. In this study, the fabrication of hydroxyapatite microspheres was carried out by converting bioglass microspheres in phosphate solution and a mixed solution of phosphate and carbonate. Bioglass microspheres were obtained through the flame spray spheroidization method with the basic raw material of bioglass particles measuring 63 μm – 200 μm. The SEM image shows that the bioglass microspheres were successfully fabricated with an average size of 76.84 μm. The conversion of bioglass microspheres was carried out by varying the observation time for 14 days. The results of the X-ray diffraction pattern showed that calcium phosphate microspheres for both solutions were formed on day 7 and there was an apatite carbonate (CHA) phase in the conversion test results in a mixed solution of phosphate and carbonate. The SEM image on the conversion results shows that the size of the calcium phosphate microspheres has the same range as the size of the bioglass microspheres before the conversion test, which is 73.59 μm – 90.83 μm. The EDX results inform that calcium phosphate microspheres have a Ca/P ratio close to the literature.
Keyword: Bone Graft, Carbonate Apatite, Flame Spray Spheroidization, SEM-EDX, XRD