Judul: The Polymorphisms of Calpastatin Gene (CAST) and Its Association with Meat Characteristics and Growth Traits in Bali Cattle
Abstrak: Bali cattle (Bos javanicus) is known as one of Indonesian native cattle that domesticated from banteng. Bali cattle was registered by the Ministry of Agriculture of Republic Indonesia (325/Kpts/OT.140/1/2010) is native Indonesian cattle. High adaptability to the environment is one of the traits belong to bali cattle. As one of the local native cattle, bali cattle have the potential to produce high quality meat. One of the factors that affecting the meat quality is genetic factor. The calpastatin gene (CAST) is one of the genes that play an important role in meat quality. The aim of this study was to analyze the polymorphisms of SNP g.41139G>A in the CAST gene using PCR-RFLP and its association with meat charactheristics and growth traits in bali cattle. Bali cattle (n=52) in this study were from BPTU-HPT Sapi Bali Denpasar. Belgian Blue cattle (n=30) from BPTU-HPT Padang Mangatas, West Sumatra Province; Wagyu cattle (n=7) and Limousin cattle (n=14) from BET Cipelang, West Java Province; PO cattle (n=30) from VBC Kebumen were used as comparison. The SNP g.41139G>A polymorphisms on CAST gene were identified by the PCR-RFLP method. Allelic frequency, genotypic frequency, and heterozygosity were analyzed to find out the genetic diversity of bali cattle. The genetic equilibrium in the population was analyzed using the Hardy-Weinberg equilibrium test (χ2) using the PopGen 1.32 program. The association of SNP g.41139G>A polymorphisms on CAST gene with meat characteristics (BFT, LDT, MS, and PIMF) using ultrasound method and growth traits (BW, WW, WT12, WT18, WT24, WT30, and ADG) were analyzed by the t test using SAS program 9.4. The SNP g.41139G>A on CAST gene was specific only found in bali cattle. The SNP g.41139G>A was polymorphic and produced GA genotype and GG genotype, while genotype AA was not found in bali cattle. The SNP g.41139G>A were monomorphic in the other Indonesian beef cattle (Belgian Blue cattle, Wagyu cattle, PO cattle, and Limousin cattle) because only found GG genotype. The results of the SNP association study of g.41139G>A on the CAST gene did not significantly effect with meat characteristics and growth traits in bali cattle., Sapi Bali (Bos javanicus) dikenal sebagai salah satu sapi asli Indonesia hasil domestikasi banteng. Sapi Bali telah ditetapkan oleh Kementerian Pertanian Republik Indonesia (325/Kpts/OT.140/1/2010) sebagai sapi asli Indonesia. Sebagai salah satu sapi asli Indonesia, sapi Bali memiliki potensi sebagai penghasil daging yang cukup berkualitas. Salah satu faktor yang memengaruhi kualitas daging yaitu faktor genetik. Gen calpastatin (CAST) merupakan salah satu gen yang berperan penting terhadap karakteristik dan kualitas daging. Penelitian ini bertujuan menganalisis keragaman SNP g.41139G>A pada gen CAST dengan menggunakan PCR-RFLP serta hubungannya dengan karakteristik daging dan pertumbuhan pada sapi Bali. Sapi Bali (n=52 ekor) yang digunakan berasal dari BPTU-HPT Sapi Bali Denpasar. Sebagai perbandingan digunakan sapi Belgian Blue (n=30 ekor) yang berasal dari BPTU-HPT Padang Mangatas, sapi Wagyu (n=7 ekor) dan Limousin (n=14 ekor) yang berasal dari BET Cipelang, sapi PO (n=30 ekor) dari VBC Kebumen. Karakteristik daging meliputi tebal lemak punggung (BFT), tebal Longissimus dorsi (LDT), marbling score (MS), dan persentase intramuscular fat (PIMF) diukur dengan menggunakan teknik ultrasonografi dan hasil ultasonografi dianalisis
Keyword: bali cattle, CAST gene, polymophisms, ultrasonography