Judul: Inokulasi fungi mikoriza arbuskula untuk meningkatkan produktivitas dan mutu benih cabai (Capsicum annuum L.) serta efisiensi penggunaan pupuk P
Abstrak: Pepper production has not been achieving optimal results although demands are increasing. The low production of pepper is partly because the use of seed that has not qualified in quantity and quality. Besides that, pepper is cultivated on the marginal soil such as Ultisol. Nutrient availability, such as P, within the marginal soil is very limited whereas there is high fixations of P. Phosporus provide the important role to increase the quality and production of the seeds. One of the efforts to boost the availability and to make the using of P efficient is inoculating Arbuscular Mycorrhizal Fungus (AMF). This research is aimed to isolate indigenous AMF from hot pepper plant rhizosphere, effective AMF selection, and to examine AMF effectiveness in enhancing production and quality of pepper seeds while the use of fertilizer whether or not efficient was then observed. Research conducted contains of four experiments which each experiment links to another. Those are: [1] Isolation, characterization and purification of AMF for three places of hot pepper plantation; [2] Selection of AMF effective in increasing the growth of hot pepper; [3] Examination of AMF effectiveness in boosting up the production and quality of pepper seeds and using efficiency of P fertilizer, [4] Plant response to inoculation inoculum of indigenous mixed AMF and AMF Mycofer. The experiment was conducted in the laboratory and greenhouse Silviculture, Faculty of Forestry IPB, Forests and Environmental Biotech Laboratory, Research Centre for Biological Resources and Biotechnology IPB, and the PPPPTK Pertanian greenhouse. The soil samples were observed coming from the three planting sites rhizosphere hot pepper. Selections are made to the effective AMF isolates from single spore culture results and AMF Mycofer from laboratory Biotech Forest and Environment, Research Centre for Biological Resources and Biotechnology IPB. Testing the effectiveness of using the design plots are divided (split-split plot) in a Randomized design pattern, with three factors and three replications. The main plot is hot pepper cultivar: Laris type from Panah Merah and Tegar from Surabumi. The sub plot is the inoculant arbuscular mycorrhizae which are uninoculated and inoculated of Mycofer AMF 100 spores per seedling. The sub-sub plot are some level of P205 dose: without fertilizer P and with 100 kg/ha of P2O5, 125 kg/ha P2O5, 150 kg P2O5. Experiment of plant response to inoculation of indigenous mixed AMF and Mycfer AMF was arranged in split plot design, the main plot: hot pepper cultivar. The sub plot is the inoculant arbuscular mycorrhiza: uninoculated, inoculated of Mycofer AMF 100 spores per seedling, and inoculated of indigenous mixed AMF. The results showed that the pepper rhizosphere is found in three genus nine species spores i.e. two species of Glomus, three species of Gigaspora and four species of Acaulospora. Based on a single spore culture, it was found that only three species that can grow and develop properly; one species of Glomus spores (Glomus sp-1), one species of Gigaspora (Gigaspora sp-1) and one species of Acaulospora (Acaulospora sp-3). The three species of these spores are propagated for experimental material II. In AMF effective selection, spores of Mycofer showed better results than
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