Judul: Evaluation of the use of chicken pituitary extract on growth performance and gonadosomatic index of giant gourami (Osphronemus goramy)
Abstrak: Ikan gurami (Osphronemus goramy) merupakan salah satu komoditas unggulan perikanan budidaya yang dikenal mempunyai laju pertumbuhan yang lambat. Proses kematangan gonad ikan ini juga membutuhkan waktu yang lama. Penelitian ini bertujuan untuk mengevaluasi penggunaan ekstrak kelenjar hipofisa ayam broiler terhadap performa pertumbuhan dan indeks gonadosomatik ikan gurami. Penelitian ini menggunakan rancangan acak lengkap dengan 6 perlakuan dan 3 ulangan. Perlakuan tersebut berdasarkan ukuran, yakni berukuran kecil K (10.3 ± 2.20 g) dan besar B (23 ± 3.88 g), serta injeksi hormon pertumbuhan rekombinan (RGH) dan ekstrak hipofisa ayam broiler (EHA). Perlakuannya adalah kontrol ikan K tanpa injeksi (K-KTR), ikan K diberi injeksi RGH 1 μg g-1 bobot ikan (K-RGH), ikan K diinjeksi dengan EHA 250 mg kg-1 bobot ikan (K-EHA), kontrol ikan B tanpa injeksi (B-KTR), ikan B dinjeksi RGH 1 μg g-1 bobot ikan (BRGH) dan ikan B diinjeksi EHA 250 mg.kg-1 bobot ikan (B-EHA). Hasil penelitian menunjukkan performa pertumbuhan (rerata bobot dan biomassa) terbaik diperoleh pada perlakuan B-RGH dan B-EHA. Hasil ini sejalan dengan ekspresi gen insulinelike growth factor 1 yang lebih tinggi (P<0.05) pada jam ke-4 pasca pemberian pakan. Injeksi RGH dan EHA cenderung meningkatkan nilai HSI dan VSI pada ikan ukuran besar. Indeks gonadosomatik tertinggi ditunjukkan oleh perlakuan BEHA. Injeksi EHA menunjukkan pengaruh positif pada performa pertumbuhan dan indeks somatik ikan dengan ukuran besar, Giant gourami was one of the competitive commodities in fisheries culture which known has a slowly growth rate. Gonadal development also needs a long rearing time. This study aims to evaluate the use of chicken pituitary gland extract on growth and gonadosomatic index of giant gourami. The study was designed using completely randomized design consisted of 6 treatments and 3 replications. The treatment based on size, which is small or K (10.3 ± 2.20) and big or B (23 ± 3.88 g), also an injection of recombinant growth hormone (RGH) and chicken pituitary gland extract (EHA). The treatment consisted of control without injection in small size (K-KTR), size K with injected 1 μg g-1 RGH per g fish body (K-RGH), size K with injected 250 mg kg-1 pituitary extract per g fish body (K-EHA), size B without injection (B-KTR), size B with injected 1 μg g-1 RGH per g fish body (BRGH), size B with injected 250 mg kg-1 pituitary extract per g fish body (B-EHA). The result of this study indicated that best growth performance (weight average and biomass) was at B-RGH and B-EHA. This result was consistent with insulin- like growth factor 1 expression which higher at the 4th hour after feeding. Pituitary gland extract and recombinant growth hormone injection can increase hepatosomatic index and visceral somatic index in B fish group size. The highest gonadosomatic index was shown by B-EHA. The pituitary gland extract injection indicated a positive effect on growth and somatic index of giant gourami size B
Keyword: chicken pituitary, giant gourami, growth, reproduction, chicken pituitary, giant gourami, growth, reproduction