Judul: The effect of recombinant growth hormone  administration on the growth performance of java barb (Barbonymus gonionotus)  juveniles at different stocking densities.
Abstrak: Ikan tawes menjadi salah satu ikan konsumsi bernilai ekonomis dan harganya terjangkau oleh masyarakat. Laju pertumbuhan dan tingkat kelangsungan hidup mempengaruhi produktivitas budidaya. Tujuan penelitian ini adalah menguji efek pemberian pakan dengan hormon pertumbuhan rekombinan ikan kerapu kertang (rElGH) terhadap respons pertumbuhan benih ikan tawes pada padat tebar yang berbeda. Penelitian ini menggunakan rancangan acak faktorial dengan dua faktor perlakuan, yaitu penggunaan rElGH (0 mg/kg dan 2 mg/kg) dan perbedaan padat tebar (40 ekor/m3 , 80 ekor/m3 dan 160 ekor/m3 ) dengan tiga kali ulangan. Benih ikan tawes (bobot 2,33 ± 0,002 cm; panjang 4-5 cm) dipelihara menggunakan hapa berukuran 1,0 × 1,0 × 1,0 m3 dengan ketinggian air sekitar 60 cm. Ikan dipelihara selama 60 hari dengan metode pemberian pakan at satiation menggunakan pakan komersial (kadar protein 31-33%). Pemberian rElGH dilakukan pada 7 hari pertama selama pemeliharaan dan dilanjutkan dengan pemberian pakan tanpa rElGH. Hasil analisis Two-way ANOVA menunjukkan penggunaan rElGH dan padat tebar serta interaksinya terdapat perbedaan yang signifikan pada kelangsungan hidup (SR), pertumbuhan bobot mutlak (PBM), laju pertumbuhan spesifik (LPS), konversi pakan (FCR) dan pertambahan biomassa. Perlakuan terbaik terdapat pada pemberian rElGH padat tebar 40 (R40) dengan SR 100%, LPS 3,58 %/hari, PBM 8,75 g, dan FCR 1,12. Namun, berdasarkan selisih pertambahan biomassa perlakuan R80 terhadap kontrolnya menunjukkan nilai tertinggi (sekitar 110 g). Kombinasi penggunaan rElGH dan peningkatan padat tebar secara signifikan meningkatkan produksi biomassa ikan tawes sehingga dapat berkontribusi pada Tingkat produksi nasional., The Java Barb has become one of economically valuable and affordable consumption fish among the community. Growth rate and survival rate affect the productivity of cultivation. The purpose of this research was to examine the effect of feeding with recombinant giant grouper growth hormone (rElGH) on the growth response of java barb fish fry at different stocking densities. This study utilized a factorial randomized design with two treatment factors: rElGH application (0 mg/kg and 2 mg/kg) and different stocking densities (40 fish/m3 , 80 fish/m3 , and 160 fish/m3 ) with three replications. Java barb fry (body weight 2.33 ± 0.002 cm; length 4-5 cm) were reared using a 1.0 × 1.0 × 1.0 m3 net cage with approximately 60 cm water depth. The fish were maintained for 60 days with at satiation feeding using commercial feed (protein content 31-33%). The rElGH-coated feed was administered during the first 7 days of the rearing period followed by feeding without rElGH. Results of the two-way ANOVA analysis indicated significant differences due to rElGH application, stocking density, and their interaction on survival rate (SR), absolute weight gain (AWG), specific growth rate (SGR), feed conversion ratio (FCR), and biomass increment. The best treatment results were obtained in R40 with 100% SR, SGR of 3.58 %/day, AWG of 8.75 g, and FCR of 1.12. However, based on biomass increment difference, R80 treatment showed the highest value (approximately 110 g) compared to its control. The combination of rElGH application and increased stocking density significantly enhanced the biomass production of java barb fish, thereby potentially contributing to national production level.
Keyword: padat tebar, hormon pertumbuhan rekombinan, rgh, tawes, intensifikasi