Judul: Synthesis and Characterization of HA-CHA/Collagen/Alginate and HA-CHA/Collagen/PVA/Alginate Composite  Scaffolds
Abstrak: Metode pencangkokan tulang yang sudah dikenal saat ini diantaranya yaitu autograft, allograft, dan xenograft. Meskipun metode tersebut terbukti dapat memperbaiki suatu jaringan tulang yang rusak, namun metode ini masih memiliki banyak keterbatasan yang dapat menyebabkan terhambatnya proses pengobatan. Salah satu upaya untuk mengatasi keterbatasan tersebut yaitu dengan dikembangkannya bone graft sintetis (scaffold). Fokus penelitian ini yaitu menyintesis scaffold komposit HA-CHA/Kolagen/Alginat dan HA CHA/Kolagen/PVA/Alginat menggunakan metode freeze-drying. Scaffold dibuat dengan memvariasikan komposisi komponen anorganik (HA, CHA) dan komponen organik (polimer) dengan rasio perbandingan anorganik:organik yaitu 25:75, 50:50, 75:25 dan HA:CHA masing-masing 100:0, 75:25, 50:50, 25:75, 0:100. Hasil sintesis kemudian dikarakterisasi menggunakan SEM-EDX, FTIR, dan XRD. Hasil SEM menunjukkan scaffold komposit HA-CHA/Kolagen/Alginat memiliki struktur morfologi cukup teratur dan pori-pori yang terbentuk tampak jelas, sedangkan scaffold komposit HA-CHA/Kolagen/PVA/Alginat memiliki struktur morfologi yang tidak teratur dan pori-pori tampak halus. Hasil pengujian EDX menunjukkan sebagian besar scaffold tersusun atas unsur karbon (C), oksigen (O), kalsium (Ca), dan fosfor (P). Hasil uji FTIR menunjukkan adanya keidentikan senyawa yang dihasilkan dan tidak terbentuk gugus fungsi baru. Hasil analisis XRD menunjukkan semakin besar kandungan organik dalam sampel akan memperkecil ukuran kristalit dan derajat kristalinitas dari scaffold., The methods of bone grafting that are known today are autograft, allograft, and xenograft. Although these method is proven to repair damaged bone tissue, but these techniques still have many limitations that can lead to inhibition of the treatment process. These limitations can be overcome by using a synthetic bone graft (scaffold). The focus of this study is synthesizing HA-CHA/Collagen/Alginate and HA-CHA/Collagen/PVA/Alginat composite scaffold using freeze-drying methods. Scaffold made by varying the composition between inorganic components (HA, CHA) and organic components (polymers) with inorganic:organic ratio is 25:75, 50:50, 75:25 and HA:CHA respectively 100:0, 75:25, 50:50, 25:75, 0:100. The synthesis results are characterized by SEM-EDX, FTIR, and XRD. The SEM results showed that the HA-CHA/Collagen/Alginate composite scaffold has a fairly regular morphological structure and the pores formed are clearly visible, while the HA-CHA/Collagen/PVA/Alginate composite scaffold has an irregular morphological structure and the pores look smooth. The EDX results test showed that most scaffolds are composed of the elements carbon (C), oxygen (O), calcium (Ca), and phosphorus (P). The results of the FTIR test showed the compounds produced are identical and do not form a new functional group. The results of XRD analysis showed that the greater organic content in the sample will reduce the size of the crystalline and crystallinity of the scaffold.
Keyword: Bone graft, composite scaffold, freeze-drying, inorganic-organic