Judul: Fermentation modification of acid hydrolisates of euchema cottonii to produce bioethanol using Saccharomyces cerevisiae and Pachysolen tannophilus.
Abstrak: Euchema cottonii is a red algae that is formed from linear polysaccharide commonly called "carrageenan". Carrageenan is galactan (polysaccharide sulfate linear hydrophilic) which is a polymer consists of galactose. The research objective is to modify bioethanol fermentation by batch condition from seaweed (Euchema cottonii) acid hydrolisates to produce a high yield of bioethanol. The study was conducted in two stages. The first stage is a preliminary phase which consists of size reduction, drying, characterization of E. cottonii with parameters tested were carbohydrates (cellulose and hemicellulose), protein, crude fiber, ash, and water content. The last stage is the preliminary were hydrolysis of E. cottonii using sulfuric acid so that the cellulose and hemicellulose components of E. cottonii can be hydrolyzed into constituent sugars (galactose) and then filtered the results so obtained solids hydrolysis and hydrolyzate containing galactose. The second stage is the stage of primary research which is divided into two stages, adaptation stages and fermentation stages. In the adaptation phase, yeast was adapted in media containing galactose to condition the yeast in media containing galactose. The second stage is the fermentation of acid hydrolisates of E. cottonii to produce bioethanol using two kinds of yeast that has been adapted (Saccharomyces cerevisiae IPBCC AL IX and Pachysolen tannophilus IPBCC AC IX). For the fermentation process, the medium of acid hydrolisates of E. cottonii added vitamins, nitrogen, and minerals (trace elements) to spur the growth of activity in the yeast consumes galactose to produce bioethanol. Euchema cottonii has high polysaccharides content and those can be converted into fermentable sugar through acid or enzymatic hydrolysis. Polysaccarides of red algae Euchema cottonii consists of 29.45% (db) carbohydrates, 3.21% (db) hemicelluloses, and 11.30% (db) celluloses. Type of sugar was obtained in acid hydrolisates of Euchema cottonii consists of 4.95% galactose, 0.25% glucose, 0.04% xylose, and 0.02% maltoheptaosa. The highest ethanol production from adapted Saccharomyces cerevisiae IPBCC AL IX was 2.38% (v/v ethanol in fermentation broth), with 79.09% substrate efficiency and 56.30% fermentation efficiency for 6 days incubation. The highest ethanol production from adapted Pachysolen tannophilus IPBCC AC IX was 0.11% (v/v ethanol in fermentation broth), with 15.39% substrate efficiency and 2.60% fermentation efficiency for 4 days incubation. The highest ethanol production with refreshed culture treatment using adapted Pachysolen tannophilus IPBCC AC IX after fermentation lasts 24 hours by adapted Saccharomyces cerevisiae IPBCC AL IX was 0.81% (v/v ethanol in fermentation broth), with 82.42% substrate efficiency and 19.79% fermentation efficiency for 6 days incubation. The highest ethanol production with refreshed culture treatment using adapted Saccharomyces cerevisiae IPBCC AL IX after fermentation lasts 24 hours by adapted Saccharomyces cerevisiae IPBCC AL IX was 1.76% (v/v ethanol in v fermentation broth), with 53.06% substrate efficiency and 43.53% fermentation efficiency which fermented for 6 days. The highest ethanol production with different of reducing sugar concentration treatment on media of fermentation after 6 days incubation was 2.13% (v/v ethanol in fermentation broth), with 76.31% substrate efficiency and 50.97% fermentation efficiency with reducing sugar consentration on media in the start of fermentation was 6.57% (w/v).
Keyword: bioethanol, yeast adaptation, Euchema cottonii, Pachysolen tannophilus, Saccharomyces cerevisiae, refreshed culture treatment, reducing sugar consentration treatment