Judul: Application of Bacillus cereus BR2, Honey, and the combination of both for prevention of Streptococcus agalactiae infection in saline nile tilapia (Oreochromis niloticus)
Abstrak: Ikan nila merupakan komoditas unggulan yang dibudidayakan secara intensif dan berpeluang terserang penyakit Streptococcus agalactiae. Upaya pencegahan yang dapat dilakukan untuk mengurangi dampak serangan bakteri S. agalactiae adalah dengan pakan suplementasi menggunakan probiotik, prebiotik, dan sinbiotik. Penelitian ini bertujuan untuk menguji pengaruh pemberian probiotik Bacillus cereus BR2, prebiotik madu randu, dan gabungan keduanya melalui pakan untuk pencegahan infeksi S. agalactiae. Penelitian ini terdiri atas lima perlakuan dan tiga ulangan yaitu A (B. cereus BR2 109 CFU mL-1 1%), B (madu randu 1%), C (B. cereus BR2 109 CFU mL-1 1% + madu randu 1%), K+ dan K- (tanpa suplementasi). Ikan uji yang digunakan adalah ikan nila nirwana 3 dengan bobot rata-rata 15,13±1,74 g. Ikan diaklimatisasi terlebih dahulu ke air payau secara bertahap hingga salinitas 10 ppt dalam akuarium berukuran 60×30×40 cm3 dengan kepadatan 10 ekor per akuarium (450 ekor m-3 ). Selama 30 hari pemeliharaan, ikan diberi pakan komersil yang telah disuplementasi sebanyak 3 kali sehari sekenyangkenyangnya. Hasil penelitian menunjukkan bahwa tingkat kelangsungan hidup, kinerja pertumbuhan, dan respon imun ikan nila salin yang diberi perlakuan suplementasi pakan probiotik B. cereus BR2 dan prebiotik madu randu lebih baik dan berbeda nyata (P<0,05) dibandingkan perlakuan kontrol. Pemberian sinbiotik B. cereus BR2 dan madu melalui suplementasi pakan dapat meningkatkan kinerja pertumbuhan dan respons imun ikan ikan nila salin., Tilapia is the main commodity that is cultivated intensively and has the potential to be infected by Streptococcus agalactiae. Prevention efforts that can be done to reduce the impact of S. agalactiae attack are by supplementing feed using probiotics, prebiotics, and synbiotics. This study aimed to examine the effect of giving Bacillus cereus BR2 probiotic, honey prebiotic, and a combination of both through the feed for the prevention of S. agalactiae infection. This study consisted of five treatments and three replications, namely A (B. cereus BR2 109 CFU mL-1 1%), B (kapok honey 1%), C (B. cereus BR2 109 CFU mL-1 1% + kapok honey 1 %), K+ and K- (without supplementation). The test fish used were nile tilapia with an average weight of 15.13±1.74 g. The fish were first acclimatized to brackish water gradually to 10 ppt. salinity in an aquarium measuring 60×30×40 cm9 with a density of 10 fish per aquarium (450 fish m-3 ). During the 30 days of rearing, the fish were given commercial feed which had been supplemented 3 times a day as full as possible. The results showed that the survival rate, growth performance, and immune response of saline tilapia which were treated with B. cereus BR2 probiotic feed supplementation and prebiotic kapok honey were better and significantly different (P<0.05) than the control treatment. The application of B. cereus BR2 and kapok honey synbiotic through feed supplementation can improve growth performance and immune response of saline tilapia.
Keyword: Bacillus cereus BR2, honey, Streptococcus agalactiae, tilapia, ikan nila salin, madu