Judul: Identification of Leaf Agronomic and Physiological Characters of Several Genotypes of Cassava (Manihot esculenta Crantz)
Abstrak: Daun ubi kayu menjadi salah satu sayur konsumsi harian sehingga peningkatan kualitas dan kuantitas daun ubi kayu perlu dilakukan melalui pemuliaan tanaman dengan peningkatan keragaman ubi kayu. Departemen AGH IPB memiliki beberapa genotipe mutan stabil hasil iradiasi sinar gamma yang berpotensi menjadi varietas baru penghasil daun. Tujuan penelitian ini untuk memilih genotipe unggul tanpa merusak daun melalui pembelajaran karakter agronomi dan fisiologi daun beberapa genotipe ubi kayu serta mempelajari korelasi antara kandungan klorofil dengan hasil klorofil meter SPAD (Soil Plant Analysis Development). Penelitian dilakukan dengan mengamati tinggi tanaman, lebar tanaman, indeks luas daun (ILD) daun ke-5, jumlah cabang dan tingkat hijau daun pada 35 genotipe yang berasal dari 6 tetua. Genotipe unggul yang diperoleh melalui pengolahan hasil pengamatan antara lain G2D12223 (tanaman asal Ratim), G3D2413 (tanaman asal UJ-5), G21533 dan G31515D (tanaman asal Gajah). Keempat genotipe tersebut diuji kandungan klorofil untuk melihat pengaruh genotipe pada klorofil dan korelasi antara tingkat hijau daun hasil SPAD dengan kandungan klorofil. Hasil uji anova dan uji lanjut menunjukkan genotipe memberikan pengaruh pada tingkat hijau daun, klorofil b, rasio klorofil a:b dan karoten. Genotipe yang unggul adalah G31515D untuk semua karakter kecuali rasio klorofil a:b dan G21533 untuk karakter rasio klorofil a:b. Hasil uji korelasi menunjukkan tingkat hijau daun berkorelasi nyata terhadap total klorofil, klorofil a, klorofil b, rasio klorofil a:b dan karoten sedangkan antosianin tidak berkorelasi dengan semua karakter uji lainnya. Kata kunci: cassava, dikotil, genotipe, karoten, klorofil, Cassava leaves are one of the vegetables for daily consumption so the quality and quantity of cassava leaves should be improved through plant breeding by increasing the diversity of cassava. The AGH Department of IPB has several stable mutant genotypes resulted from gamma-ray irradiation which have the potential to become new leaf-producing varieties. The purpose of this study was to select superior genotypes without damaging the leaves through studying the agronomic and physiological characters of several cassava genotypes and studying the correlation between chlorophyll content and the results of the chlorophyll meter SPAD (Soil Plant Analysis Development). The research is conducted by observing plant height, plant width, leaf area index (LAI) of the 5th leaf, number of branches, and leaf green level in 35 genotypes from 6 parents. The superior genotypes obtained through processing the observations included G2D12223 (plants from Ratim), G3D2413 (plants from UJ-5), G21533, and G31515D (plants from Gajah). The four genotypes were tested for chlorophyll content to see the effect of the genotype on chlorophyll and the correlation between the green level of the leaves from the SPAD results and the chlorophyll content. ANOVA test results and further tests showed that the genotype affected the level of leaf green, chlorophyll b, the ratio of chlorophyll a: b and carotene. The superior genotype was G31515D for all characters except chlorophyll a: b ratio and G21533 for chlorophyll a: b ratio characters. The results of the correlation test showed that the level of leaf green was significantly correlated with total chlorophyll, chlorophyll a, chlorophyll b, the ratio of chlorophyll a: b, and carotene, while anthocyanin is not correlated with all other test characters. Keywords: cassava, chlorophyll, carotene,
Keyword: cassava, chlorophyll, carotene, dicotyledoneae, genotype