Judul: Analysis of Meat Quality Determinants Genes as Markers of Premium Meat  Producing  in  Bali  Cattle
Abstrak: Bali cattle are native Indonesian livestock genetic resources (AnGR) that have been domesticated from Banteng. Bali cattle should be maintained and preserved for future use because they have the potential to become premium quality beef cattle. Bali cattle's potential can be increased through selection based on genetic markers or marker-assisted selection (MAS), which produces more accurate, effective, and efficient results. The process can be applied to candidate genes related to meat quality. Calpain1 (CAPN1), fatty acid banding protein-4 (FABP4), sterol regulating element binding protein-1 (SREBF1), and scavenger receptor class B member 1 (SCARB1). The purpose of this study is to analize candidate genes that determine beef quality through analysis of gene diversity using DNA sequencing methods and to associate gene diversity with quality characteristics of Bali beef from Kupang, as well as to analyze CAPN1 gene expression. In this study, 95 Bali cattle were used. Meat samples were used for physical analysis and fatty acid composition, and blood samples were used to identify the diversity of the CAPN1, FABP4, SREBF1, and SCARB1 genes using the direct-sequencing method. Analysis of CAPN1 gene expression in Bali cattle liver and blood tissue. Six novel SNPs were discovered for the CAPN1 gene, four for the FABP4 and SREBF1 genes, and five for the SCARB1 gene in Bali cattle as a result of the analysis of gene diversity. The marbling score, intramuscular fat, pH, meat color, tenderness (WBSF), and fatty acid composition were associated with the CAPN1 gene (g.5327C>T, g.5869T>C, and g.5959C>T), according to the results of the association analysis. The FABP4 gene (g.5002C>T) was significantly associated with carcass characteristics including longissimus dorsi thickness (TLD). SREBF1 gene (g.12629T>C, g.12731T>C, g.12881A>G, and g.12986C>T) had an association with fatty acid composition. The SCARB1 gene (g.72219C>T, g.72380C>A, g.72400A>G, g.72517G>A and g.72607C>T) were significantly associated with fatty acid composition. The results of gene expression analysis showed that the low tenderness value (WBSF) was characterized by a higher CAPN1 gene expression level. The results of this study can be concluded that the CAPN1, FABP4, SREBF1, and SCARB1 genes are potential candidate genes to be used as genetic markers for selection of meat quality traits in Bali cattle., Sapi Bali merupakan sumber daya genetik ternak (SDGT) asli indonesia yang telah mengalami proses domestikasi dari Banteng. Sapi Bali berpotensi untuk dikembangkan sebagai sapi pedaging kualitas premium, oleh karena itu perlu dipertahankan dan dilestarikan untuk dimanfaatkan secara berkelanjutan. Potensi sapi Bali dapat ditingkatkan melalui seleksi berdasarkan marka genetik atau marker assisted selection (MAS) yang hasilnya lebih akurat, efektif dan efisien. Proses seleksi dapat dilakukan pada kandidat gen-gen yang berkaitan dengan kualitas daging. Beberapa gen yang potensial untuk diseleksi antara lain: gen calpain1 (CAPN1), gen fatty acid banding protein-4 (FABP4), gen sterol regulating element binding protein-1 (SREBF1), dan scavenger reseptor class B member 1 (SCARB1). Penelitian ini bertujuan menganalisis karakteristik karkas dan daging berdasarkan teknik ultrasonografi, sifat fisik, dan profil asam lemak daging sapi Bali, mengkarakterisasi kandidat gen penentu kualitas daging sapi melalui analisis keragaman gen dengan metode sekuensing DNA dan mengasosiasikan keragaman gen dengan sifat kualitas daging sapi Bali dari Kupang, serta menganalisis ekspresi gen
Keyword: bali cattle, gene expression, meat quality, ultrasound