Judul: Penyisipan Gen LEAFY Lengkap Tanaman Kakao pada Vektor Ekspresi dan Transformasi ke dalam Agrobacterium sp.
Abstrak: Cacao (Theobroma cacao L.) is one of the plantations that has important role for the national economy. However, one of the problems faced in the cocoa plantations is cherelle wilt. Cherelle wilt reduces the level of productivity of cocoa. Flowering genes regulate the normal flower formation in cacao. One of the genes that play a major role in the flower formation is the LEAFY gene. Studying the flowering genes are expected to elucidate the effect of these genes to cherelle wilt. The objective of this research is to construct expression vector harbouring fulllength of LEAFY gene. The fulllength of LEAFY gene that has been inserted into the expression vector was then transformed into Agrobacterium tumefaciens AGL-0. Cloning of LEAFY gene into expression vector was carried out using the Gateway technology. The full size LEAFY gene of 1200 bp was first inserted into a donor vector and then inserted into the expression vector. Characterization of expression vector inserted with LEAFY gene was carried out using the PCR plasmid technique which resulted in a DNA band of 500 bp. Expression vector inserted with LEAFY gene was transformed into the Agrobacterium tumefaciens AGL-0. Analysis of recombinant plasmid after transformation into the Agrobacterium tumefaciens AGL-0 using PCR plasmid technique result in a DNA band of 500 bp. The conclusion of this research is that the fulllength of LEAFY gene had successfully been inserted into the expression vector with Gateway technology.
Keyword: 

Judul: Isolasi dan Karakterisasi Fragmen Gen LEAFY dari Bantalan Bunga Kakao
Abstrak: Cocoa plant productivity in Indonesia is still low. Information and knowledge at the molecular level about the process of development of regenerative organs of cocoa can be an effective solution to problems associated with the regenerative low productivity of cocoa plants.LEAFY gen is key gene in the shoot meristem transition to flower one. The purposes of the research were to isolate LEAFY gene from cacao’s flower cushion and analyse its expresion in several cacao’s tissues. Isolation of LEAFY gene using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) technique showed that three spesific primer pairs designed based conserved area can amplify the target gene. Futhermore this fragment were inserted into pGEM-T Easy vector then transformed into competent cell E. Coli XL-1 Blue. The result of bioinformatic analysis showed that the fragment of target gene has a high homology to LEAFY gene of other plants in gene bank’s database. Characterization of target gene was initiated with RNA isolation phase from several cacao’s tissues then those were amplified with RT-PCR technique. Amplification at 62 ºC of annealing temperature for 33 cycles was successful to show the existence of LEAFY gene in several cacao’s tissues isolated.
Keyword: 

Judul: Efektivitas Program Siaran Radio Manajemen Qalbu (MQ) dalam Pengembangan Masyarakat Muslim.
Abstrak: Radio Manajemen Qalbu (MQ) hadir ditengah masyarakat dengan membawa nuansa yang berbeda dibandingkan dengan radio lainnya. Radio MQ memiliki segmentasi khalayak yaitu keluarga muslim Indonesia sebagai poros terbentuknya perubahan bagi umat muslim ke arah yang lebih baik. Hal ini ditunjukkan dengan sajian program siaran positif dan mengarah pada pembinaan keluarga dan keagamaan dengan menjadikan Islam sebagai sumber informasinya. Secara tidak langsung, fenomena tersebut telah menciptakan ruang untuk mengembangkan masyarakat khususnya dalam pengetahuan keislaman. Penelitian ini bertujuan untuk menganalisis faktor apa saja yang berhubungan dengan keterdedahan pada siaran radio serta menganalisis hubungan keterdedahan pada siaran radio terhadap efektivitas program siaran Radio MQ dalam pengembangan masyarakat muslim. Metode penelitian ini menggunakan kombinasi pendekatan kuantitatif dengan sensus kepada 26 responden dan kualitatif dengan wawancara mendalam kepada informan. Analisis data menggunakan uji korelasi Rank Spearman. Hasil penelitian menunjukkan bahwa terdapat efektivitas siaran radio dalam pengembangan masyarakat muslim. Faktor yang berhubungan dengan hal ini adalah keterdedahan pada siaran radio berupa lama mendengarkan dengan tingkat pengetahuan pendengar.
Keyword: pengembangan masyarakat, radio, siaran muslim