Judul: Karakterisasi Gen Prolaktin (PRL) pada Ayam Kampung Ayam Walik Ayam Leher Gundul dan Ayam Arab
Abstrak: Indigenous and local chicken germ plasm in Indonesia have a high potency to be developed. One of the potency that can be developed is an improvement egg production. Low egg production of those chickens can be increased by selecting the in broodiness. Broodiness is controlled by prolactin gene (PRL). Since the characteristics of PRL gene of kampung, walik, leher gundul and arab are still limited refence, therefore this study aim to investigate the characteristic of PRL gene on their. Blood samples were extracted to obtain DNA. PCR then was used to amplified the fragment of PRL. A total of 20 samples successfully amplified at annealing temperature of 60 °C for 45 seconds and then directly sequenced. DNA sequencing results were analyzed using the MEGA 5 program. PRL gene in this study had similarity of 98%-99% with the PRL gene sequence data available on GenBank. This study also shows that there were 11 point mutation of PRL gene’s fragment with the type of mutations were deletions, insertions, transitions and transvertion. Those mutation types have potency as new single nucleotide polymorphisms (SNPs).
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Judul: Genetic Observation of the External Body of Local Breed Chickens (Gallus gallus domesticus) in Various Districts
Abstrak: Ayam kampung (Gallus gallus domesticus) merupakan ayam asli Indonesia yang pada saat ini telah tercemar dengan gen ayam ras unggul Eropa terutama ayam yang dipelihara secara ekstensif tradisional. Penelitian ini bertujuan menghitung keaslian ayam kampung di berbagai wilayah kecamatan di Kabupaten Kulon Progo dan Kabupaten Bogor. Karakteristik genetik eksternal yang diamati adalah warna bulu, pola bulu, corak bulu, kilau bulu, warna shank, dan bentuk jengger. Karakteristik tersebut dianalisis menggunakan analisis frekuensi gen dominan dan resesif otosomal, analisis frekuensi gen alel ganda, analisis frekuensi gen dominan dan resesif tertaut seks, analisis frekuensi kandungan gen ayam kampung asli Indonesia, dan analisis frekuensi gen asli. Hasil penelitian menunjukkan ayam kampung di Kecamatan Galur, Kecamatan Panjatan, Kecamatan Megamendung, dan Kecamatan Cibungbulang memiliki tingkat keaslian kurang dari 50%. Ayam kampung di Kecamatan Cibungbulang memiliki tingkat keaslian paling rendah, sedangkan ayam kampung di Kecamatan Panjatan memiliki tingkat keaslian paling tinggi pada penelitian ini., Kampong chicken (Gallus gallus domesticus) is an indigenous Indonesian chicken that is currently contaminated with genes of European superior breeds of chickens, especially chickens that are reared extensively traditionally. This study aims to quantify the authenticity of native chickens in various districts in Kulon Progo Regency and Bogor Regency. The external genetic characteristics observed were feather color, feather pattern, feather pattern, feather luster, shank color, and cockscomb shape. These characteristics were analyzed using autosomal dominant and recessive gene frequency analysis, double allele gene frequency analysis, sex-linked dominant and recessive gene frequency analysis, gene content frequency analysis of Indonesian native chickens, and native gene frequency analysis. The results showed that native chickens in Galur Subdistrict, Panjatan Subdistrict, Megamendung Subdistrict, and Cibungbulang Subdistrict had an authenticity level of less than 50%. The native chicken in Cibungbulang subdistrict has the lowest level of authenticity, while the native chicken in Panjatan subdistrict has the highest level of authenticity in this study.
Keyword: Ayam kampung, keragaman, frekuensi gen, kualitatif

Judul: Cycle Hamilton pada Graf Lengkap, Graf Regular, dan Graf 2-Connected 4-Regular Berorder Kurang dari Sepuluh
Abstrak: Suatu graf disebut graf Hamilton apabila graf tersebut memuat cycle Hamilton, yaitu cycle yang melewati setiap vertex tepat satu kali. Menentukan keberadaan cycle Hamilton pada suatu graf dapat menggunakan syarat cukup yang berupa teorema-teorema yang telah diperkenalkan sejak tahun 1950-an. Beberapa syarat cukup yang dibahas dalam karya ilmiah ini adalah syarat cukup yang berhubungan dengan graf lengkap dan graf regular. Suatu graf lengkap berorder 𝑛������ ≥ 3 dan graf r-regular berorder 𝑛������ ≤ 2𝑟������ + 1 merupakan graf Hamilton, Karena kedua graf tersebut memuat cycle Hamilton. Selanjutnya, syarat cukup tersebut digunakan untuk menentukan keberadaan cycle Hamilton pada graf 2-connected 4- regular berorder kurang dari 10.
Keyword: graf Hamilton, graf regular, graf lengkap, graf 2-connected 4-regular