Judul: In silico Analysis of Active Compounds in Ketepeng Cina Plant (Cassia alata) as an Anti-Candida Candidate
Abstrak: Penelitian ini mengevaluasi potensi senyawa aktif dari tanaman ketepeng cina (Cassia alata) sebagai kandidat obat anti-candida menggunakan pendekatan in silico. Studi ini bertujuan mengidentifikasi dan menganalisis interaksi senyawa aktif asal ekstrak tanaman ketepeng cina dan menginteraksikannya dengan enzim lanosterol 14a-demetilase, yang merupakan target utama dalam terapi infeksi candidiasis. Metode yang digunakan meliputi penambatan molekuler (molecular docking) untuk menentukan afinitas dan stabilitas interaksi ligan dengan reseptor. Hasil menunjukkan bahwa senyawa stigmasterol berpotensi sebagai inhibitor enzim lanosterol 14a-demetilase. Stigmasterol mampu berinteraksi dengan sisi aktif Tyr126 pada reseptor dengan nilai energi afinitas yang lebih negatif dibandingkan dengan gentian violet sebagai ligan pembanding., This study evaluates the potential of active compounds from the ketepeng cina (Cassia alata) plant as an anti-candida drug candidates using an in silico approach. The study aims to identify and analyze the interactions of active compounds from ketepeng cina extract and the enzyme lanosterol 14a-demethylase, a primary target in candidiasis therapy. The methods used include molecular docking to determine the affinity and stability of ligand-receptor interactions. The results indicate that stigmasterol compound has potential as inhibitor of the lanosterol 14a-demethylase enzyme. Stigmasterol can interact with the active site Tyr126 on the receptor with more negative affinity energy values compared to gentian violet as the reference ligand.
Keyword: Cassia alata, In silico, anti-candida, ketepeng cina, lanosterol 14a-demetilase, lanosterol 14a-demethylase

Judul: Dugaan 4’-Hidroksi-6-C-xilofuranosil Flavanon dari Fraksi Polar Ekstrak Metanol Daun Ketepeng (Cassia alata)
Abstrak: Cassia alata, known as ketepeng in Indonesia, is an herb that has been traditionally used as ointment. The purpose of this study is to isolate and to identify compounds from polar fraction of the methanol extract from the leaves. Crude methanol extract of ketepeng leaves was fractionated briefly by liquid -vacuum chromatography. The eluates having the same separation pattern on thin-layer chromatography were combined and the fractions were grouped based on their polarity. H fraction eluted with methanol as eluent was fractionated further by gravitational column chromatography resulting 21 fractions. H9 fraction which showed single TLC spot with 3 different eluent mixtures, was further partitioned and then analyzed by using proton nuclear magnetic resonance (1H NMR) spectrophotometer and liquid chromatograph-mass spectrometer (LC-MS). The LC-MS chromatogram indicated a mixture of 4 compounds. Based on 1H NMR and LC-MS spectrum, the most abundant fraction in the chromatogram was predicted as a flavonoid C-glycoside compound, that is 4’-hydroxy-6-C-xylofuranosyl flavanone.
Keyword: ketepeng, flavonoid, flavanone, Cassia alata

Judul: Pola Reproduksi Babi
Abstrak: Pubertas pada babi jantan umumnya dapat dicapai pada umur 5 sampai 7 bulan dengan variasi 4 sampai 8 bulan. Babi jantan muda sebaiknya dibiarkan mencapai umkur 8 sampai 9 bulan sebelum digunakan untuk menagwini betina. Pada babi betina pubertas dicapai pada umur 5 sampai 8 bulan dengan rata-rata 6 bulan. Sedangkan umur yang dianjurkan untuk perkawinan pertama adalah 8 sampai 10 bulan.
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