Judul: Kualitas Spermatozoa Cauda Epididimis Domba Garut yang diberi Pakan Limbah Tauge dan Indigofera sp pada Pengencer Tris Kuning Telur
Abstrak: Feed is one of the important factor to improve the quality of spermatozoa. Feed can be made from waste bean sprout and legume Indigofera sp. Garut ram has high economic value, but it is faced with the problem of limited number of a good quality of male. One of method that can be used to solve a problem is the utilization of cauda epididymal sperm since it has motility and an able to fertilize oocytes as well as ejaculated sperm. The purpose of this research was to determine the effect of feeding (waste bean sprout and Indigofera sp) to increase the quality of garut ram sperm. This research used 8 garut male with age around 11 months and had been raised for 3 months. They were divided into two groups, the first group was fed with 30% waste bean sprout and the second group was fed with 30% Indigofera sp. Parameter of semen quality was analyzed as follow: for the volume, color and consistency were expressed by descriptively, while testis weight, sperm concentration and cytoplasmic droplet were tested by T-test and followed by regression test for correlation between testis weight and sperm concentration. Sperm motility, sperm viability, integrity of sperm plasma membrane and secondary abnormality were analyzed using Completely Randomized Design, Faktorial (2x4) with four replication. The first factor was type ration (waste bean sprout and Indigofera sp) and the second factor was storage time (day 0, 1st, 2nd, 3th). Data was analyzed by analysis of variance and continued with Duncan test to observed the different between treatments. Results of the experiment showed that volume, color and consistency of semen were same in all treatments, while testis weight, sperm concentration and cytoplasmic droplet were not significant different in type ration treatments by T-test (P>0.05). There were no correlation between testis weight and sperm concentration (P>0.05) and expressed as Y = 5654.26 - 3.96X ; R2 = 0.003. Furthermore sperm viability were significant different in all treatments by analyzed of variance (P<0.05). The integrity of sperm plasma membrane and sperm motility were significant different in storage time treatment (P<0.05), but were not significant different in type ration by analyzed of variance (P>0.05). It can be summarized that both of 30% waste bean sprout and 30% Indigofera sp feed treatments were not significantly influence on all of parameter sperm quality.
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Judul: Karakteristik spermatozoa asal cauda epididymis domba postmortem pada pengecer tris kuning telur
Abstrak: Pemanfaatan spermatozoa asal cauda epididymis domba postmortem mempunyai potensi dan memberi keuntungan dalam aplikasi teknologi reproduksi. Penelitian bertujuan untuk mengetahui karakteristik spermatozoa asal cauda epididymis domba postmortem dan mengetahui daya fertilitasnya selama dalam pengencer Tris kuning telur. Spermatozoa dikoleksi dari cauda epididymis dengan metode aspirasi menggunakan spoit 3 cc yang dihubungkan dengan jarum 18 G. Spermatozoa yang terkoleksi dimurnikan melalui sentrifus pada kecepatan 1500 rpm selama 10 menit. Selanjutnya dilakukan pemeriksaan rutin spermatozoa yang meliputi konsentrasi, motolitas, persentase hidup, abnormalitas, butiran sisa sitoplasma, serta keutuhan tudung akrosom sebelum diencerkan pada pengencer Tris kuning telur. Spermatozoa yang telah diencerkan diamati keutuhan tudung akrosom dan motilitasnya selama4 hari. Hasil penelitian menunjukkan angka rataan beberapa parameter sebagai berikut: konsentrasi 835.56 x 106 sel/ml; motilitas 63.17%; persentase hidup 68.40%; abnormalitas 20.93%; tudung akrosom utuh (TAU) 44.53%; dan morfologi butiran sisa sitoplasma 6.20%. Secara statistika terdapat korelasi yang erat antara berat testis dengan konsentrasi perolehan spermatozoa (r = 0.858). Hal ini menunjukkan semakin tinggi berat testis maka semakin mudah untuk memperoleh spermatozoa dengan metode aspirasi. Di samping itu, secara statistika diperoleh korelasi yang erat antara penurunan motilitas dengan keutuhan tudung akrosom (r = 0.999). Artinya semakin rendah jumlah sperma yang motil maka semakin rendah pula spermatozoa yang mempunyai tudung akrosom utuh. Lebih lajjut ditunjukkan pula spermatozoa yang telah diencerkan dapat dipertahankan motilitas dan tudung akrosom utuh yang layak dipergunakan sampai hari ke-2 setelah pengenceran.
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Judul: : Persamaan Tunggal sebagai Representasi Kurva Komposit
Abstrak: A mathematical method is introduced to represent a composite curve based on an extension of analytic geometry. The representation is given either with a single equation or with two equations, in the case of parametric representation. This method permits the representation of composite curves in similar manner to the conventional representation of non-composite curves. Some mathematical tools, including Heaviside unit step function and periodizer function, are used in the establishment of a single equation. In this paper, regular equations of regular and irregular polygon, as well as composite curves of two dimensions, are implemented using a computer algebraic system, Mathematica.
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