Judul: Development of methodology of DNA Extraction and Purification of Feather Bird Turtledove (Geopelia striata) for Sexing and Utilization of Genetic Diversity Studies
Abstrak: In the molecular studies of birds, the total DNA obtained from the extraction and purification of blood. Bird turtledove is very small and easy to stress so it is very risky if the blood is taken. Plumage may be other alternatives in obtaining DNA because it contains epithelial cells at their bases. The purpose of this research was to obtain a methodology of extraction and purification of DNA from the feathers of birds turtledove (Geopelia stiata) to study the utilization of sexing and genetic diversity. The method used consists of three kinds: 1) Digestion buffer-based method, 2) modification method using cetyl Trimethyl Ammonium bromide (CTAB buffer), and 3) the method of using tablets InhibitEX in CTAB buffer modification. The third method were divided into two treatments, the tablet was added before the addition of a solution of phenol or InhibitEX Before Phenol (IBP) and, the tablet was added after the addition of a solution of phenol or treatment InhibitEX After Phenol (IAP). The treatment period of soaking the samples in low-TE solution was during in 3 days and 14 days. The quality of DNA bands seen from the results of electrophoresis and spectrophotometer then compared with the control DNA from blood. Based on the purification result, the DNA was not obtained from Digestion buffer method. But, the DNA was obtained in CTAB buffer method, and the method using tablets InhibitEX been obtained DNA. Value averaging the results of the second method of DNA purity was lower (1.156) compared with the third method that is 3IBP (1.321) and 3IAP (1.551). PCR process was conducted as part to evaluate whether the DNA template (DNA template) from the extraction of fur can be amplified without a hitch or not. Based on the PCR test, 3IAP method has a high percentage (100%) compared with 3IBP method (50%) and method 2 (10%).
Keyword: 

Judul: Restriction Fragment Length Polymorphism (RFLP) Gen Sitokrom B Dna Mitokondria Dari Delapan Spesies Burung
Abstrak: Penelitianinibertujuanuntuk mempelajari RestrictionFragmentLength Polymorphism (RFLP) delapanspesiesburunglokalberdasarkanfragmen Sitokrom b DNAmitokondria.Hasilyangdiperolehdapatdigunakansebagai dasarinformasiuntukmengenalispesiesburung.Delapansampeljaringanburung diperolehdisekitarBogordandisimpandalamlarutan NaCl4,5Myang mengandungDMSO25 %.DNAdiisolasidenganmetodepresipitasiamonium asetat.Primeruniversal SitokrombL14841/H15149 digunakanuntukamplifikasi fragmengen sitokrom b DNAmitokondriadenganmenggunakanmetode PolymeraseChainReaction (PCR) yangdilanjutkandenganelektroforesis.DNA hasilPCR(amplikon)kemudian dicerna (dipotong) denganmenggunakanenzim restriksiendonuklease Hinf I(2,5unit)dan Rsa I (20unit) masing-masing selama 2jamdan6jam.PenghitunganukuranDNA amplikon danfragmenhasil pemotongan dilakukandenganmengukurjarakantaramigrasiDNAsampel denganmigrasi DNAladder dalamgelagarose2%.Hasilpenelitianmenunjukkan bahwadelapanspesiesburunglokalmempunyaiamplikonyangberukuran 359pb,sertamempunyaiukuranfragmenrestriksi HinfI yangberbedakecuali bondolpekingdanmanyarjambul.Fragmenrestriksi Rsa I yangdihasilkanjuga berbedakecualimanyarjambuldandederukjawa,sertaayamkampungdan merbahcerukcuk.Berdasarkanhasilpenelitianinidapatdisimpulkanbahwa metodePCRmenggunakanprimeruniversalsitokrombsertapemotongan fragmendenganenzim restriksi HinfI dan atau Rsa I dapatdigunakanuntuk mengenali kedelapan spesiesburung. Katakunci: sitokromb, DNA mitokondria, HinfI, RsaI,burung
Keyword: 

Judul: Cycle Hamilton pada Graf Lengkap, Graf Regular, dan Graf 2-Connected 4-Regular Berorder Kurang dari Sepuluh
Abstrak: Suatu graf disebut graf Hamilton apabila graf tersebut memuat cycle Hamilton, yaitu cycle yang melewati setiap vertex tepat satu kali. Menentukan keberadaan cycle Hamilton pada suatu graf dapat menggunakan syarat cukup yang berupa teorema-teorema yang telah diperkenalkan sejak tahun 1950-an. Beberapa syarat cukup yang dibahas dalam karya ilmiah ini adalah syarat cukup yang berhubungan dengan graf lengkap dan graf regular. Suatu graf lengkap berorder 𝑛������ ≥ 3 dan graf r-regular berorder 𝑛������ ≤ 2𝑟������ + 1 merupakan graf Hamilton, Karena kedua graf tersebut memuat cycle Hamilton. Selanjutnya, syarat cukup tersebut digunakan untuk menentukan keberadaan cycle Hamilton pada graf 2-connected 4- regular berorder kurang dari 10.
Keyword: graf Hamilton, graf regular, graf lengkap, graf 2-connected 4-regular