Judul: Pengaruh pemberian zat pengatur tumbuh Rootone F terhadap keberhasilan pertunbuhan stump Shorea Stenoptera Burck
Abstrak: Tujuan penelitian adalah untuk mengetahui pengaruh pemberian zat pengatur tumbuh Rootone F dalam beberapa konsentrasi dan perbedaan rasio ukuran akar dan batang stump terhadap pertumbuhan stump Shorea stenoptera Burck. Penelitian ini dilakukan selama 3 (tiga) bulan (25 Desember 1991 sampai 23 Maret 1992) di Kebun Percobaan Pusat Penelitian dan Pengembangan Hutan Darmaga Bogor. Bahan stump untuk penelitian ini diambil dari permudaan alam anakan Shorea stenoptera Burck, di Kebun Percobaan Pusat Penelitian dan Pengembangan Hutan Haurbentes Jasinga, Bogor.
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Judul: Pengaruh pemberian ZPT (Rootone-F) terhadap pertumbuhan stek Duabanga moluccana, Blume.
Abstrak: INTRODUCTION Insufficient supply of raw materials to support wood based industries in Indonesia cause of the changes on orientation for wood supply from natural forest to plantation forest in developing fast growing tree species. Duabanga mollucana Blume, is an Indonesian native fast growing tree species that have good characteristic as raw materials for wood based industries. Vegetative propagation can be done to this species besides their ability to be propagated from seed. Shoot and stem cuttings are alternative ways to obtain qualified seedlings in appropriate amounts and timely manner. Plant growth regulator treatment was expected to increase cutting's survival rate and rooting percentage. Objective of this research was to determine the effect of plant growth regulator on the growth of shoot and stem cutting of Duabanga moluccana. This research would be useful to provide information for the production for high quality seedlings. MATERIAL AND METHOD This research was carried out on green house with KOFFCO system (Komatsu-Forda Fog Cooling System) at Balai Penelitian dan Pengembangan Hutan dan Konservasi Alam, Gunung Batu, Bogor, West Java. from August to october 2010. Materials used in this research were shoot and stem of Duabanga seedlings from selected mother trees (M07) from SEAMEO BIOTROP, Rootone-F, Dithane M-45, rice husk, cocopeat, and zeolite. The experimental design in this research was completely randomized design with 2 factors and 3 replicates. The first factor was source of cutting material (shoot and stem) and the second factor was concentration of plant growth regulator (0 ppm, 500 ppm, 1000 ppm, and 1500 ppm). Growth parameters observed were (1) survival rate, (2) rooting percentage, (3) number of roots, (4) root length, (5) root fresh weight and dry weight (6) shoot fresh weight and dry weight (7) shoot-root ratio. Data was processed with analysis of variance then followed by fisher's LSD test. RESULT AND CONCLUSION Analysis of variance was conducted to explore the impact of all factors on growth parameters, shoot cutting was found to be the most significantly different related to each growth parameters observed; those were (1) survival rate, (2) rooting percentage, (3) number of roots, (4) root length, (5) root fresh weight and dry weight (6) shoot fresh weight and dry weight (7) shoot-root ratio , beside that, stem cuttings was not significantly different related to those growth parameters observed. Rooting percentage on shoot cuttings was 82% and stem cuttings was 0%. Plant growth regulator treatments were not significantly affected cuttings, because control (0 ppm) on shoot cuttings showed high rooting percentage value (77%), followed by 500 ppm (80%), 1500 ppm (83%), and 1000 ppm (87%). Interaction between source of materials and concentration of plant growth regulators was also not significantly related on each growth parameters observed because all of stem cutting could not survive during root primordial initiation, shoot cuttings was also not significantly different related to those growth parameters observed, because control (0 ppm) on shoot cuttings produced significant survival rate (77 %).
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Judul: Purifikasi dan karakteristik imunoglobulin Y (lgY) kuning telur ayam spesifik Salmonelaenteritidis menggunakan metode sodium dedocyl sulphate poly acrilamide gel electrophoresis (SDS-page)
Abstrak: Penelitian ini bertujuan untuk mengetahui karakter berat molekul fragmen-fragmen lgY yang terbentuk dengan menggunakan metode Sodium Dodecyl Sulphate Poly-Acrylamide Gel Electrophoresis (SDS-PAGE). Bahan lgY didapatkan dari kuning telur ayam Single Comb Brown Leghorn berusia 24 minggu. Lima ekor ayam divaksinasi dengan 1 ml (10 CFU) suspensi bakteri Samonella Enteritidis yang telah dilemahkan dan lima ekor ayam yang tidak divaksinasi sebagai kontrol. Penyuntikan dilakukan pada vena Brachialis dengan pengulangan vaksinasi (booster) dilakukan pada minggu ke-2, ke-3 dan ke-4 seolah vaksinasi pertama. Satu minggu setelah vaksinasi terakhir telur dikoleksi dan diuji Agar Gel Presipitation Test (AGPT).
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