Judul: Optimasi Penggunaan IgY Dan IgG Sebagai Antibodi Penangkap Dan Pendeteksi Antigen Ekskretori Sekretori (ES) Fasciola gigantica Dalam Uji Elisa
Abstrak: Fasciolosis is a parasitic disease of domestic ruminant that cause high economic loss. Currently diagnosis of fasciolosis relies on microscopic examination of Fasciola sp. eggs in fecal sample. Serological test such as ELISA has been developed to improve the diagnostic method of fasciolosis. This study was designed to optimize the use of polyclonal antibodies (IgY and IgG) as capture antibody and detection antibody in ELISA assay to detect excretory secretory (ES) of Fasciola gigantica. The conformation was done in two models which compared IgY (9,57 mg/ml) and IgG (3,75 mg/ml) as capture antibody. The first conformation was IgY as capture antibody in 1:1000 dilution and IgG as detection antibody in 1:100, 1:1000, and 1:10000 dilutions. The second conformation was IgG as capture antibody in 1:1000 dilution and IgY as detection antibody in 1:100, 1:1000, and 1:10000 dilutions. The results showed that IgY as capture antibody in 1:1000 dilution and IgG as detection antibody in 1:100, 1:1000, 1:10000 dilutions were able to capture ES antigen 3,87; 2,85; and 1,65 times than cut off value. The other conformation with IgG as capture antibody in 1:1000 dilution was able to capture ES antigen as much as 1,91 times than cut off value with IgY as detection antibody in 1:100 dilution. This showed that the good optimation ELISA was IgY as capture antibody in 1:1000 dilution and IgG as detection antibody in 1:100 dilution.
Keyword: 

Judul: Konsentrasi IgY Ayam D2 IPB pada Berbagai Intensitas Parasit Gastrointestinal
Abstrak: Native chickens are more likely to become domesticated livestock and industrial livestock that cannot be replaced by other types of chicken. IPB-D1 chickens are a new breed of native Indonesian chickens created by cross-breeding native chickens and broiler parent stock. The IPB D1 chicken has a high concentration of immunoglobulin IgY and resistance again Newcastle disease infection. IPB D2 chickens are a potential candidate for a new line of selection results from IPB D1 chickens for disease resistance and fast growth. Helminthiasis is a disease caused by helminth infection that causes health problems such as lose weight and production decrease. Helminthiasis can reduce the immune system, so it is necessary to research to determine the IgY concentration of IPB D2 chicken in chickens with various levels of helminth infection. The study aims to investigate the IgY infection in various helminth infections. Twenty-two chickens IPB D2 chicken 27-week-old were used for this research. The faecal and blood sample were collected. Observed data is obtained from blood and faecal sample. The Ig Y titer was measured by the ELISA method, and the presence of helminth was observed by McMaster and simple flotation technique. The result shows that the IgY concentration of IPB D2 chicken was 5.6-12.36 mg/mL, 65% of the chicken was categorized as a high titer of IgY, i.e., > 7.5 mg/mL and the rest (35%) categorized low titer (< 7,5mg/mL). The egg of nematode and cestode was found in the chicken. The genus that was found is Strongyloides, Trichuris and Capillaria. Other gastrointestinal parasites include oocytes and protozoa of the genus Eimeria. Chicken with a high IgY titer has higher infection than chicken with a low IgY. It is indicated that helminth infection induces antibodies by increasing the IgY titer
Keyword: Chicken, Helminth, Immunity, IPB D2 Chicken, Ig Y

Judul: Value Proposition Canvas design of Mie Unni Kadai business
Abstrak: Kota Padang adalah salah satu kota yang memiliki posisi yang strategis untuk menjalankan usaha kuliner dengan prospek yang menguntungkan. Tingginya jumlah usaha kuliner di Kota Padang yang terus berkembang dari tahun ke tahun dapat meningkatkan persaingan. Selama enam bulan Mie Unni Kadai beroperasi, hasil yang didapatkan belum mampu mencapai target penjualan yang telah ditetapkan. Untuk mengatasi masalah tersebut dapat dilakukan analisis value propotition canvas. Penelitian ini bertujuan untuk: (1) Mengidentifikasi model bisnis yang telah Mie Unni Kadai terapkan dengan pendekatan business model canvas, (2) Menganalisis value proposition canvas pada Mie Unni Kadai, (3) Merumuskan rekomendasi strategi Mie Unni Kadai di masa yang akan datang. Penelitian ini merupakan penelitian deskriptif dengan pendekatan kualitatif. Hasil dari penelitian ini adalah terciptanya empat pembaruan yang meliputi penyediaan paket pelajar, inovasi menu, menambah level kepedasan dan bekerja sama dengan perusahaan mobile payment, tiga perbaikan pada product and services yang meliputi perbaikan dari ruangan yang terasa panas, penambahan ready stock supply dan perbaikan pada sisi interior., Padang City is one of the cities that has a strategic position to run a culinary business with favorable prospects. The increasing number of culinary businesses in the city of Padang which continues to grow from year to year could increase the competition. Since six months of opening, Mie Unni Kadai has yet reach the sales. To solve the problems, a value propotition canvas analysis can be performed. This study aims to: (1) identify the condition of the existing business model at Mie Unni Kadai by using the business model canvas, (2) analyze the value proposition canvas of Mie Unni Kadai, (3) formulate a recommendation strategy for Mie Unni Kadai for the future. This is a descriptive research with qualitative approach. The final result of this research is created four reforms which comprise student packages, menu innovations, spicy level upgrate, and collaboration with a mobile payment company, three product and service improvements involve hot room improvement, ready stock supply augment, and interior improvement.
Keyword: business model canvas, qualitative, value proposition canvas