Judul: Studi Trypsin Inhibitor dan Alfa-amylase Inhibitor pada Pohon Sengon (Paraserianthes falcataria (L) Nielsen) Provenan Banjarnegara dan Subang
Abstrak: Plantation of sengon (Paraserianthes falcataria (L) Nielsen) almost always faces the problem of attack by stem borer pest insect, referred to as boktor (Xystrocera festiva Pascoe). The damage and loss incurred by this pest is considerable. In the effort of preventing this attack by boktor there is a need for planting sengon which are resistant to attack by this pest through tree improvement program. Therefore, there is a need for research on the presence of anti pest substance in the form of trypsin inhibitor and α-amylase inhibitor in each part of the tree. Object of this research was part of sengon trees of Banjarnegara and Subang provenances in the form of leaves, bark, and wood, which were made into powder. The trees used in this study were healthy trees and trees attacked by the pest boktor, with replication of five trees for each category of tree condition. Observation of trypsin inhibitor activity was conducted using synthetic substrate benzoil-DL-arginin-p-nitroanilid (BAPNA), enzyme trypsin (SIGMA 3,5 U/mg) and natural enzyme from digestive system of boktor larvae with size of 1,5 cm. Observation of α-amylase inhibitor activity used materials of solution of soluble starch, solution of enzyme α-amylase (SIGMA 320 U/mg), and enzyme α-amylase which was extracted from larvae with size of 3,5 cm. Data were processed in Completely Randomized Design (RAL) with 3 factors, namely provenances, tree condition and parts of tree, using software SPSS 12.0 Survey results showed that larvae in bark had length and weight less than larvae in wood. Of the 5 trees of Banjarnegara provenance, boktor larvae were found only in the wood part, whereas in that of Subang provenance, boktor larvae were found in wood and bark parts. Based on analysis of variance, factor of provenance did not have significant effect on activity of trypsin inhibitor. Tree parts and tree condition had very significant effect on activity of trypsin inhibitor. Interaction between factors of provenance and tree parts gave significant effect on activity of trypsin inhibitor. Duncan advanced test showed that bark possessed highest activity of trypsin inhibitor, whereas that of the wood was the lowest. Results of analysis of variance showed that tree condition (healthy or sick trees), as well as tree parts in the form of leaves, bark and wood, had significant effect on activity of α-amylase inhibitor (AIA). On the other hand, provenance factor did not have significant effect. Duncan advanced test showed that wood was the part which had the highest average of α-amylase inhibitor activity, whereas that of the lowest, was in bark part. Interaction between tree condition and tree parts had very significant effect on the magnitude of α-amylase inhibitor activity. Analysis of variance showed that average values of trypsin inhibitor activity which were obtained using synthetic enzyme and natural enzyme from digestive system of boktor did not show significant differences. The same phenomenon was also found for provenance factor. Activity of trypsin inhibitor which was tested using synthetic enzyme was greater compared with that of natural enzyme from boktor digestive system. On the other hand, tree condition had significant effect, where the healthy
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Judul: Ekspresi Gen Trypsin Inhibitor Sengon (Falcataria moluccana Miq.) yang Sehat dan Terserang Hama Boktor
Abstrak: Sengon (Falcataria moluccana Miq.) merupakan salah satu jenis cepat tumbuh yang kayunya banyak dimanfaatkan sehingga sering dibudidayakan terutama di Pulau Jawa. Adanya serangan hama penggerek batang boktor (Xystrocera festiva Thombs) terhadap sengon menyebabkan kerugian yang besar karena dapat menurunkan kualitas kayu dan menurunkan nilai jual kayu sengon. Secara alami tanaman sengon memiliki gen ketahanan terhadap serangan hama boktor, salah satunya adalah trypsin inhibitor. Tujuan penelitian ini adalah membandingkan tingkat ekspresi gen trypsin inhibitor pada kambium pohon sengon resisten dan terserang hama boktor secara kuantitatif dengan teknik Quantitative Real Time PCR. Gen target yang digunakan yaitu trypsin inhibitor (TI) dan gen Actin sebagai internal kontrol dirancang dari analisa data transkriptom sengon. Data Threshold cycle (CT) yang diperoleh kemudian dikuantifikasi menggunakan metode comparative CT method (2 -ΔΔCT). Hasil kuantifikasi menunjukkan tingkat ekspresi gen trypsin inhibitor pada ketiga sampel sengon resisten yaitu sebesar 1.90, 2.20, dan 2,85 sedangkan sengon terserang boktor sebesar 4.07, 2.05, dan 4.08. Rata-rata ekspresi gen trypsin inhibitor pada sengon terserang hama boktor lebih tinggi dibandingkan sengon yang resisten. Kata kunci: Falcataria moluccana, Trypsin Inhibitor, Quantitative Real Time PCR, Sengon (Falcataria moluccana Miq.) is highly demanded fast growing tree species which leads to the increasing sengon cultivation especially in Java. Boktor pest (Xystrocera festiva Thombs) decrease the quality of sengon wood, causing a high loss due to lower selling price of the log. Naturally sengon has resistance gene to defend itself from boktor pest attacks, and one of these genes is trypsin inhibitor. This study aimed to comparing the level of trypsin inhibitor gene expression in sengon’s cambium that is resistant and attacked by boktor pests. The analysis of gene expression level was carried out using the Real Time PCR technique. Primers of trypsin inhibitor (TI) gene as the target gene and Actin gene as the internal control were designed from sengon’s transcriptomic data analysis. Threshold cycle (CT) that obtained then quantified using comparative CT method (2 -ΔΔCT). Quantification results showed that the expression level of trypsin inhibitor from three resistant samples are 1.90, 2.20, and 2,85 while in sengon attacked by boktor are 4.07, 2.05, and 4,08. The average trypsin inhibitor gene expression in sengon attacked by boktor pest was higher than resistant ones. Keywords: Falcataria moluccana, Trypsin Inhibitor, Quantitative Real Time PCR
Keyword: Falcataria moluccana, Trypsin Inhibitor, Real Time PCR

Judul: Strategi Social Media Marketing 4C Dalam Meningkatkan Brand Awareness Mooi Wear.
Abstrak: Pada era digital seperti sekarang media sosial tidak hanya dimanfaatkan untuk berkomunikasi dan berbagi informasi saja, namun juga untuk mengembangkan bisnis. Berbagai bisnis di Indonesia memanfaatkan media sosial sebagai media untuk memasarkan produknya. Salah satu bisnis tersebut adalah Mooi Wear. Mooi Wear melakukan pemasaran media sosial untuk meningkatkan brand awareness serta memperluas pasar. Adapun tujuan penelitian ini adalah untuk memberikan usulan strategi pemasaran media sosial 4C dalam meningkatkan brand awareness. Wawancara mendalam dengan pihak internal Mooi Wear digunakan untuk mengetahui pemasaran media sosial 4C yang telah dilakukan Mooi Wear dan pengisian kuesioner secara online oleh pelanggan dan calon pelanggan potensial. Hasil dari penelitian ini merupakan usulan pemasaran media sosial 4C berdasarkan hasil evaluasi strategi yang telah diterapkan yaitu pada aspek co-creation; inovasi produk. currency; membuat program membership dan menerapkan harga bundling dan promosi menarik. Communal activation; melakukan kolaborasi, membuat website, menjual produk dan bekerjasama dengan berbagai ecommerce. Conversation; membuat konten kreatif
Keyword: brand awareness, Mooi Wear, social media marketing