Judul: Analisis Transkriptomik Pohon Sengon (Falcataria moluccana Miq.) Rentan dan Resisten terhadap Hama Boktor (Xystrocera festiva Pascoe).
Abstrak: Sengon (Falcataria moluccana Miq.) plantations in monoculture system has increases boktor (Xystocera festiva Pascoe) pest population and distribution, however there is no effective and efficient control method yet. This study aims to analyze sengon transcriptomic data and identify expressed genes involved in sengon resistance to boktor pests, also other genes related to abiotic and biotic stresses. Unpublishe de novo transcriptomic data was acquired using the RNAseq and deposited in the DDBJ repository with accession number DRA008389. DEG analysis was perfomed using R program with edgeR package. The results of gene screening were then annotated using genes analog with the Swissprot database. DEG analysis produced 6859 upregulated contigs, genes in resistant tree were Heat shock cognate 70 kDA protein, Trypsin inhibitor, and Chalcone synthase. Based on previous research, trypsin inhibitor is a defensive mechanism of sengon against boktor pests, while other genes are known to be involved in the abiotic and biotic stress response.
Keyword: de novo transcriptomic, Falcataria moluccana Miq, trypsin inhibitor, Xystocera festiva Pascoe

Judul: Ekspresi Gen Trypsin Inhibitor Sengon (Falcataria moluccana Miq.) yang Sehat dan Terserang Hama Boktor
Abstrak: Sengon (Falcataria moluccana Miq.) merupakan salah satu jenis cepat tumbuh yang kayunya banyak dimanfaatkan sehingga sering dibudidayakan terutama di Pulau Jawa. Adanya serangan hama penggerek batang boktor (Xystrocera festiva Thombs) terhadap sengon menyebabkan kerugian yang besar karena dapat menurunkan kualitas kayu dan menurunkan nilai jual kayu sengon. Secara alami tanaman sengon memiliki gen ketahanan terhadap serangan hama boktor, salah satunya adalah trypsin inhibitor. Tujuan penelitian ini adalah membandingkan tingkat ekspresi gen trypsin inhibitor pada kambium pohon sengon resisten dan terserang hama boktor secara kuantitatif dengan teknik Quantitative Real Time PCR. Gen target yang digunakan yaitu trypsin inhibitor (TI) dan gen Actin sebagai internal kontrol dirancang dari analisa data transkriptom sengon. Data Threshold cycle (CT) yang diperoleh kemudian dikuantifikasi menggunakan metode comparative CT method (2 -ΔΔCT). Hasil kuantifikasi menunjukkan tingkat ekspresi gen trypsin inhibitor pada ketiga sampel sengon resisten yaitu sebesar 1.90, 2.20, dan 2,85 sedangkan sengon terserang boktor sebesar 4.07, 2.05, dan 4.08. Rata-rata ekspresi gen trypsin inhibitor pada sengon terserang hama boktor lebih tinggi dibandingkan sengon yang resisten. Kata kunci: Falcataria moluccana, Trypsin Inhibitor, Quantitative Real Time PCR, Sengon (Falcataria moluccana Miq.) is highly demanded fast growing tree species which leads to the increasing sengon cultivation especially in Java. Boktor pest (Xystrocera festiva Thombs) decrease the quality of sengon wood, causing a high loss due to lower selling price of the log. Naturally sengon has resistance gene to defend itself from boktor pest attacks, and one of these genes is trypsin inhibitor. This study aimed to comparing the level of trypsin inhibitor gene expression in sengon’s cambium that is resistant and attacked by boktor pests. The analysis of gene expression level was carried out using the Real Time PCR technique. Primers of trypsin inhibitor (TI) gene as the target gene and Actin gene as the internal control were designed from sengon’s transcriptomic data analysis. Threshold cycle (CT) that obtained then quantified using comparative CT method (2 -ΔΔCT). Quantification results showed that the expression level of trypsin inhibitor from three resistant samples are 1.90, 2.20, and 2,85 while in sengon attacked by boktor are 4.07, 2.05, and 4,08. The average trypsin inhibitor gene expression in sengon attacked by boktor pest was higher than resistant ones. Keywords: Falcataria moluccana, Trypsin Inhibitor, Quantitative Real Time PCR
Keyword: Falcataria moluccana, Trypsin Inhibitor, Real Time PCR

Judul: Faktor-faktor yang Berkaitan Erat dengan Respon Kepala Keluarga Terhadap Program Wajib Belajar Pendidikan Dasar Sembilan Tahun (Wajar 9) (Kasus di Komunitas Rural Kota (KRK) di Kotamadya Bogor dan Kasus Komunitas Semi Urban Desa (KSUD) di Kabupaten Bogor, Propinsi Jawa Barat)
Abstrak: Tujuan penelitian yang ingin dicapai adalah 1) Mengetahui respon kepala keluarga terhadap Program Wajib Belajar Pendidikan Dasar 9 tahun, dan 2) Mengetahui faktor-faktor yang berkaitan erat dengan respon kepala keluarga terhadap Program Wajib Belajar Pendidikan Dasar 9 tahun. Penelitian ini dilakukan di dua wilayah yang berbeda secara administratif dan berbeda ciri masyarakatnya, yaitu Komunitas Rural Kota (KRK) yaitu Komunitas RW IV dan V Kelurahan Tegalega, Kecamatan Bogor Tengah, Kotamadya Bogor yang menurut administratif termasuk kota (urban) tetapi masih berciri desa (rural). Sementara satu lagi adalah Komunitas Semi Urban Desa (KSUD) yaitu Komunitas Dusun Cisasah, Situ Uncal dan Cihideung Desa Pumasari yang terletak di ujung Selatan Kecamatan Dramaga, Kabupaten Bogor yang menurut administratif termasuk desa (rural) tetapi sudah berciri kota (urban).
Keyword: