Judul: Molecular Docking of Potential Microbial Enzyme as Palm Oil Mill Effluent Bioremediation Agents
Abstrak: Palm oil mill effluent (POME) yang berasal dari industri kelapa sawit merupakan salah satu penyebab utama pencemaran air karena kaya akan bahan organik dan warnanya yang gelap. Limbah POME memiliki beberapa zat penyebab sifat uniknya, salah satunya adalah melanoidin. Tidak hanya melanoidin, komponen fenolik yang terdapat pada POME juga diketahui bersifat fitotoksik bagi lingkungan akuatik. Beberapa enzim mikroba potensial sudah dianggap mampu mendegradasi bahan kimia ini, tetapi mekanismenya tidak diketahui. Melalui penelitian ini, enzim potensial tersebut (α-glukosidase, β-glukosidase, β-mannanase, lakase, dan mangan katalase) digunakan untuk memvisualisasikan pengikatan kompleks reseptor-ligan dalam mencari agen bioremediasi POME yang sesuai. Analisis penelitian dilakukan dengan Autodock Vina dan skor penambatan mewakili kekuatan afinitas. Hasil penelitian menunjukkan bahwa melanoidin yang berkontribusi terhadap warna gelap POME memiliki nilai docking terbaik yaitu -10,1 kkal/mol dengan mangan katalase. Hasil akhir menunjukkan bahwa semua reseptor memiliki afinitas tinggi terhadap melanoidin, yang menunjukkan bahwa enzim ini melakukan reaksi spontan terhadap komponen kimia limbah POME, Palm oil mill effluent (POME) originated from palm oil industries is one of the significant causes of water pollution because of its richness in organic matters and dark color. It has been discovered that POME has several substances that cause its distinct characteristics, namely melanoidin. Several phenolic components found in POME are also known to be phytotoxic for the aquatic environment. Certain potential microbial enzymes are already presumed capable of degrading these chemicals, but the exact mechanism is unknown. Here I employed molecular docking of these possible enzymes (i.e., α-glucosidase, β-glucosidase, β-mannanase, laccase, and manganese catalase) to visualize the ligand-receptor complex binding in searching for POME potential bioremediation agent. The result analysis uses the Autodock Tool v1.5.6, and the docking score represents the affinities of the bond strength. The results show that melanoidin has the lowest docking score with -10.1 kcal/mol into manganese catalase. The results demonstrate that all of the receptors possess a high affinity towards melanoidin, indicating that these enzymes perform spontaneous reactions towards POME waste chemical components
Keyword: Bioremediation, Microbial enzymes, Molecular docking, POME waste

Judul: Aplikasi Konsorsium Mikroorganisme untuk Mendegradasi Lignin dalam Limbah Cair Pabrik Kelapa Sawit pada Kondisi Aerobik
Abstrak: The microorganism consortia from eight different ponds of palm oil mill effluents (POME) at Palm Oil Mills (POM) of PT Condong Garut and Rejosari- Lampung were selected and applied to degrade lignin of lignosellulosic materials in POME under an aerobic condition. They were aerated to ensure effective isolation of aerobic microorganisms. Selection of lignin-degrading fungi consortia were determined by using selected agar plate (LAC) and liquid Lignin Chloramphenicol (LC) medium. Their ability to degrade lignin of lignocellulose materials in POME were shown by absorbance value and total solids in broth culture. Microorganism consortia from Anaerobic-4 at POM of Condong Garut had the high growth rate in liquid LC medium. These result was also confirmed by their consortia’s ability had the highest degrade of lignin materials in POME compared with the others selected consortia.
Keyword: aerobic, microorganisms consortia, lignin, POME

Judul: Perilaku Remaja dalam Mendengarkan Radio Komunitas (Kasus Pendengar BeTe Radio di Kelurahan Tegal Gundil, Kecamatan Bogor Utara, Kota Bogor, Provinsi Jawa Barat)
Abstrak: This research aimed to analyze the relation between teenager behaviors on community radio listening activity. This research goals are (1) to identify the pattern of listening activity on community radio among teenager (2) to analyze the relation between listeners characteristics and broadcast format assessment by listening community radio (3) to analyze the relation between community radio listeners pattern with the teenager behaviors, and (4) to identify the listeners expectations towards community radio. The main method of this research is quantitative approach, and qualitative approach as supporting. Research and observationshow that the teenagers who are listening to the BeTe community radio have low level of listening activity pattern. Affective level and social network were also relatively low. Related to the listeners characteristics, the research result showed that gender does not have any relation with the pattern of radio listening activity. Beside that, age, media ownership, and main activities have significant relation towards pattern of radio listening activity. To assess broadcast format variable, pshycological proximity does not have any relation significantly with the pattern of radio listening activity. In the pattern of radio listening activity variable which is consist of listening frequency, listening duration, program choices, participation on the program have significant relation with to point of view, affective, and social network of BeTe Radio listeners.
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