Judul: Studi Histopatologi Manfaat Ekstrak Jintan Hitam (Nigella sativa) pada Organ Pencernaan Ayam Broiler
Abstrak: Black cumin or Nigella sativa is a plant belonging to herbal medicine which is famous in the world. The research was conducted to study the histopathological changes of digestive organs (proventriculus, ventriculus, duodenum, and pancreas) in broiler chickens after administration of N. sativa. A total of 100 chickens (one day old chick / DOC) were divided into 3 treatment groups, namely control (K) group, vaccinated ND, and vaccinated IBD; group A vaccination with ND, IBD, AI virus and treated by extracts N. sativa; group B given ND, IBD virus vaccine, and extracts N. sativa. Extracts N. sativa was given for 5 weeks every day with the oral administration route. Every week since the second week up to sixth week chickens drawn at randomly to be analyzed histopathological, chicken were necropsied and organ samples were collected for a later fixed with Neutral Buffered Formalin (BNF) 10% and processed into histopathology slides stained with Haematoxylin Eosin (HE). The results showed that the extract of N. sativa reduced number of inflammatory cells in proventriculus; decrease of ventriculus koilin thickening while decreasing the number of inflammatory cells of ventriculus; increased the percentage of intact epithelium, increasing the number and extensive of intestinal villi; and increasing the number of bowel crypts; increase the number active acinar of pancreas and increase the body weight. This result indicate that extracts of N. sativa has the protective potency on gastrointestinal tract of broiler chickens and reduce the number of the inflammatory cells.
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Judul: Pengaruh Pemberian Ekstrak Minyak Jinten Hitam (Nigella sativa) Terhadap Gambaran Mikroskopis Paruparu Mencit (Mus musculus)
Abstrak: This study was aimed to get information about the effect of blackseed (Nigella sativa) effect on the histopathology of mice lung. Twelve male mice of 4 weeks old were divided into four groups, each group consisted of three mice. Group K or negative control received aquadest, group HS 0.1 was received 0.1 ml blackseed oil, group HS 0.2 was received 0.2 blackseed oil and group HS Madu was received 0.3 ml mixed of blackseed oil and honey (1:20) for two months. At the end of the experiment, the mice were euthanized by atlanto occipital dislocation and necropsied to collect the lung sample. The lungs were processed to prepare the histopathology slides with Hematoxyllin-Eosin and Periodic Acid Schiff (PAS) stain. The parameters observed include counting percentage of bronchus and bronchial presenting exudates, counting the goblet cell between the respiratory epithelial cell, calculating the BALT area, counting the limfoid cell density of the BALT, counting the congestion, hemorrhage, inflammation and emphysema area on the certain fields of view, measuring the thickness of smooth muscle surround bronchial using Image J® for Microsoft® Windows®. Quantitative data were analyzed using SPSS® 16.0 software and ANOVA test followed Duncan test. The result showed that Nigella sativa treatment does not lead to increase exudation in the bronchus and bronchioles lumen,a decrease in the activity of goblet cells, a decrease the BALT area and an increase in the lymphoid cell density,a reduce the area of congestion and hemorrhage, inflammation and emphysema, reduction at the smooth muscle thickness. The blackseed content such thymoquinone and longifolene were thought to provide a favorable effect on lung histomorphology.
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Judul: Cycle Hamilton pada Graf Lengkap, Graf Regular, dan Graf 2-Connected 4-Regular Berorder Kurang dari Sepuluh
Abstrak: Suatu graf disebut graf Hamilton apabila graf tersebut memuat cycle Hamilton, yaitu cycle yang melewati setiap vertex tepat satu kali. Menentukan keberadaan cycle Hamilton pada suatu graf dapat menggunakan syarat cukup yang berupa teorema-teorema yang telah diperkenalkan sejak tahun 1950-an. Beberapa syarat cukup yang dibahas dalam karya ilmiah ini adalah syarat cukup yang berhubungan dengan graf lengkap dan graf regular. Suatu graf lengkap berorder 𝑛������ ≥ 3 dan graf r-regular berorder 𝑛������ ≤ 2𝑟������ + 1 merupakan graf Hamilton, Karena kedua graf tersebut memuat cycle Hamilton. Selanjutnya, syarat cukup tersebut digunakan untuk menentukan keberadaan cycle Hamilton pada graf 2-connected 4- regular berorder kurang dari 10.
Keyword: graf Hamilton, graf regular, graf lengkap, graf 2-connected 4-regular