Judul: Identifikasi Gen Aroma Pada BC5F1 Ciherang-Pandan Wangi dan BC4F1 Ciherang-Mentik Wangi
Abstrak: The demand of high quality rice becomes increasingly in Asian market and around the world. The increase in productivity and quality of Indonesian agriculture has continued, such as improving new aromatic rice variety to have good characteristics (resistant to pest, ) which is like non-aromatic rice. The aim of this research was to develop new varieties of non transgenic aromatic rice by induction of aroma gene (mutated badh2) from aromatic varieties (Pandan Wangi or Mentik Wangi) to non aromatic varieties rice (Ciherang). Rice BC5F1 CP (Ciherang/Pandan Wangi) and BC4F1 CM (Ciherang/Mentik Wangi) that used in this research were the result from previous research with site-directed crossing method. Selection of plant was done by PCR with RM223 marker for CP and Bradbury marker for CM. Visualization of DNA sample by PCR was done by using gel agarose 3% for CP and 2% for CM. DNA amplification result of BC5F1 CP with RM223 marker can distinguish the band pattern of aromatic and non aromatic with band size 140 bp and 160 bp. In addition, DNA amplification result of BC4F1 CM with Bradbury marker produced DNA band size 580 bp, 355 bp and 257 bp. The succeed in introgression of aroma gene from Pandan Wangi or Mentik Wangi to Ciherang was showed by heterozygote band.
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Judul: Identifikasi Gen badh2 termutasi Pada Padi BC4F1 Ciherang-Mentik Wangi (CM) dan BC5F1 Ciherang- Pandan Wangi (CP)
Abstrak: Development of aromatic rice varieties based non-transgenic required to obtain an aromatic rice with a high potential. The objectives of this experiment were to study of introduction of aromatic trait from aromatic into nonaromatic rice through crossing and to screen the plants by using molecular markers linked to aromatic trait. Pandan Wangi and Mentik Wangi will be used as aromatic donor parents meanwhile Ciherang are as recurrent parents. Pandan Wangi and Mentik Wangi will be crossed with Ciherang to produce aromatic hybrids. PCR-based markers that will be used in this research i.e. Bradbury primer for detecting the aromatic trait in Ciherang x Mentik Wangi and RM223 primer for Ciherang x Pandan Wangi. Result of the research showed that BC4F1 and BC5F1 hybrid plants have been obtained from both of the crossing combination. Screening of the plants showed that the tested plants have been proved carrying the aromatic trait indicated by the formation of DNA band heterozygous with size 580, 355, and 257 bp for Ciherang x Mentik Wangi and 140 and 160 bp for Ciherang x Pandan Wangi.
Keyword: Pandan Wangi, Mentik Wangi, Ciherang, badh2

Judul: Cycle Hamilton pada Graf Lengkap, Graf Regular, dan Graf 2-Connected 4-Regular Berorder Kurang dari Sepuluh
Abstrak: Suatu graf disebut graf Hamilton apabila graf tersebut memuat cycle Hamilton, yaitu cycle yang melewati setiap vertex tepat satu kali. Menentukan keberadaan cycle Hamilton pada suatu graf dapat menggunakan syarat cukup yang berupa teorema-teorema yang telah diperkenalkan sejak tahun 1950-an. Beberapa syarat cukup yang dibahas dalam karya ilmiah ini adalah syarat cukup yang berhubungan dengan graf lengkap dan graf regular. Suatu graf lengkap berorder 𝑛������ ≥ 3 dan graf r-regular berorder 𝑛������ ≤ 2𝑟������ + 1 merupakan graf Hamilton, Karena kedua graf tersebut memuat cycle Hamilton. Selanjutnya, syarat cukup tersebut digunakan untuk menentukan keberadaan cycle Hamilton pada graf 2-connected 4- regular berorder kurang dari 10.
Keyword: graf Hamilton, graf regular, graf lengkap, graf 2-connected 4-regular