Judul: Analisis Plateletcrit dan Red Blood Cell Distribution Width pada Uji Toksisitas Subkronis Ekstrak Kapang Endofit Daun Sirsak (Annona muricara L.)
Abstrak: Penelitian sebelumnya menemukan adanya aktivitas antikanker ekstrak kapang endofit daun sirsak (Annona muricata L.) terhadap sel kanker payudara MCF-7. Uji toksisitas subkronis perlu dilakukan sebelum dapat masuk ke fase uji klinis. Penelitian ini bertujuan mengukur nilai plateletcrit (PCT) dan red blood cell distribution width (RDW) pada tikus betina Sprague-Dawley yang diinduksi dengan variasi dosis ekstrak kapang endofit untuk melihat toksisitasnya. Tikus dibagi menjadi enam kelompok, yaitu kelompok normal, kelompok perlakuan dengan dosis 20, 80, dan 240 mg/kg bb, serta kelompok satelit dengan satelit normal dan satelit dosis 240 mg/kg bb. Kelompok satelit digunakan untuk melihat adanya efek tertunda. Hasil uji ANOVA menunjukkan tidak terdapat perbedaan nyata pada parameter PCT dan RDW antara kelompok normal dengan kelompok yang diinduksi sediaan uji. Data kelompok satelit juga tidak menunjukkan adanya perbedaan nyata. Simpulan dari penelitian ini adalah ekstrak etil asetat kapang endofit amat dikonsumsi selama 90 hari terhadap parameter RDW dan PCT., Previous studies showed that the endophytic mold extracted from soursop leaves (Annona muricata L.) has an anticancer ability against MCF-7 breast cancer cells. A subchronic toxicity test was needed before proceeding into the clinical trial. This study aimed to compare the value of plateletcrit (PCT) and red blood cell distribution width (RDW) on female Sprague-Dawley rats induced with endophytic mold extract with various doses to see its toxicity. The rats were grouped into six groups, the control group, groups that were given the extract with doses of 20, 80, and 240 mg/kg bw, as well as satellite groups that consist of normal satellite group and satellite group which was induced with 240 mg/kg bw dose of extract. The satellite group was used to show any delayed toxicity effect. ANOVA test for both parameters showed no significant difference between the control group and the group induced with endophytic mold extract. The satellite group also didn’t show any significant difference for both parameters. It can be concluded that the ethyl acetate extract of endophytic mold from soursop leaves is safe to be consumed for 90 days in regards to the PCT and RDW parameters.
Keyword: endophytic fungi, plateletcrit, red blood cell distribution width, toxicity

Judul: Blood Examination in Subchronic Toxicity Test Combination of Bidara Laut Extract (Strychnos ligustrina) and Dihydroartemisinin-Piperaquine Phosphate (DHP) as Antimalarial
Abstrak: Kombinasi ekstrak bidara laut dan dihidroartemisinin-piperakuin fosfat (BL-DHP) terbukti efektif sebagai antimalaria, namun belum diketahui toksisitasnya. Penelitian ini merupakan bagian pengujian toksisitas subkronis pada mencit jantan dan betina selama 28 hari. Metode pengujian dilakukan berdasarkan peraturan BPOM RI menggunakan 3 tingkatan dosis yaitu dosis rendah, sedang, dan tinggi. Parameter pada penelitian ini berupa gambaran hematologi, biokimia, dan elektrolit darah dianalisis menggunakan one way ANOVA-Duncan test. Hasil gambaran hematologi meliputi eritrosit, hemoglobin, hematokrit, PDW, limfosit, dan granulosit berbeda signifikan dengan kelompok perlakuan kontrol, sementara pada kelompok satelit tidak berbeda signifikan. Gambaran biokimia darah meliputi SGPT dan SGOT berbeda signifikan pada dosis sedang dan tinggi, namun bersifat reversibel pada kelompok satelit. Gambaran elektrolit tidak berbeda signifikan pada kelompok perlakuan maupun satelit. Berdasarkan uji hematologi, biokimia, dan elektrolit darah, kombinasi BL-DHP aman digunakan pada dosis rendah (200mg/KgBB ekstrak bidara + 111 mg/kgBB DHP), namun perlu dilakukan pengamatan parameter lainnya seperti tingkat kematian dan histopatologi organ., The combination of S.ligustrina (bidara laut) and dihydroartemisininpiperaquine phosphate (BL-DHP) proved as effective antimalarial, but the toxicity degree is not yet known. This study is the part of subchronic toxicity test in male and female mice for 28 days and tested using NA-DFC guidelines with low, medium, and high doses. The parameters in this study, hematology, biochemical, and electrolyte features of blood, were analyzed by one way ANOVA-Duncan test. Hematological features include erythrocytes, hemoglobin, hematocrit, PDW, lymphocytes, and granulocytes were significantly different from control group, while the satellite group was not significantly different. Biochemical features included SGPT and SGOT were significantly different at medium and high doses, but reversible in satellite group. Electrolyte features did not show significant changes in treatment and satellite group. Based on hematology, biochemical, and electrolyte test, BL-DHP is safe at low dose (200mg/KgBW bidara extract + 111 mg/kgBW DHP), but mortality rate and organ histopathology need to be monitored.
Keyword: antimalarial, dihydroartemisinin-piperaquine phosphate, hematology, S.ligustrina, subchronic toxicity

Judul: Optimasi Penjadwalan Armada Pesawat Terbang
Abstrak: One of the common issues faced by airline companies relies on fulfilling passenger demands for Origin-Destination (O-D) routes using all fleet types. This paper presents an optimization model to determine fleet assignment for each flight route in order to minimize total costs. The total costs consist of the operating cost and passenger spill cost. The model is considered as an integer linear programming which is further implemented within the Citilink airlines schedule, and was solved using software Lingo 11.0. The model produced a fleet assignment in order to fulfill demand for all O-Ds with minimum cost.
Keyword: fleet assignment, integer linear programming, optimization