Judul: DNA Fragmentation on HCT-116 Colon Cancer Cell in the Presence of Short Chain Fatty Acid (SCFA) Extract
Abstrak: Type 3 resistant starch (RS3) is retrogaded starch that resists digestion in the small intestine of healthy people and fermented in the large intestine. The main fermentation products are the short chain fatty acids (SCFA) acetate, propionate, and butyrate. Butyrate is the major energy source for the colonocytes and have anti-carcinogenic effects mainly by affecting proliferation, differentiation, and apoptosis of colonocytes. The aim of this study was to analyze apoptotic DNA fragments of HCT-116 human colon cancer cell incubated with SCFA derived from fermentation of RS3 of sweet potato (Ipomoea batatas) by C. Butyricum BCC B2571 using DNA ladder assay. The DNA bands detected from attached cells treated with SCFA containing 1.00 and 1.25 mM butyrate were different from the control, appeared as longer smear. There were separate DNA bands in the floating cells in the treated medium (SCFA containing 0.625, 1.00, and 1.25 mM butyrate) at ±100 bp. These bands were not presence in the control. DNA fragmentation was not clear in the attached cells, but was observed as a separate bands in the floating cells in the medium which contained the dead cells. This research still needs optimazion of DNA extraction to get the best result of DNA fragmentation assay
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Judul: Fractionation of Active Compounds of  Matah Merah Snail Extract (Cerithidea obtusa) as Anticancer
Abstrak: Beragam upaya dilakukan untuk mengembangkan obat alternatif penyakit kanker dari bahan alam. Penggunaan bahan alam perairan tersebut dapat mengunakan biota keong matah merah. Keong matah merah memiliki aktivitas biologis seperti antidiabetes, antioksidan serta antikanker. Tujuan penelitian untuk mendapatkan fraksi keong matah merah yang memiliki aktivitas antikanker pada sel HeLa dan MCF-7 dan menentukan golongan komponen aktif pada fraksi terpilih. Metode yang digunakan antara lain ekstraksi maserasi, fraksinasi, separasi dengan kromatografi lapis tipis dan kromatografi kolom vakum, Microtetrazolium (MTT) assay, dan analisis fitokimia. Subfraksi etil asetat keong matah merah yang mengandung senyawa bioaktif diantaranya alkaloid, saponin, flavonoid, fenol hidrokuinon serta steroid dan triterpenoid. Rendemen fraksi tertinggi yaitu pada fraksi etil asetat 9,02%. Aktivitas antikanker menunjukkan fraksi etil asetat memiliki aktivitas tertinggi dalam menghambat pertumbuhan sel kanker HeLa dengan nilai IC50 19,58 μg/mL dan nilai IC50 MCF-7 31,51 μg/mL. Fraksi etil asetat keong matah merah berpotensi dikembangkan sebagai bahan alam alternatif antikanker.
Keyword: Antikanker, Fraksinasi, Keong Matah Merah, Anticancer, Fractionation, Matah Merah Snail

Judul: Management of Maintenance, Utilization and  Economic Value of the Javan Eagle at the Bogor Indonesian Safari Park
Abstrak: Elang jawa merupakan satwa dilindungi oleh Pemerintah Indonesia dan IUCN dengan kategori Endangered (IUCN 2016). Elang jawa terus terancam sehingga dipelukan upaya konservasi eksitu, salah satunya dilakukan di Taman Safari Indonesia (TSI) Bogor. Penelitian ini bertujuan mengidentifikasi manajemen pemeliharaan, pemanfaatan, dan nilai ekonomi burung elang jawa di TSI Bogor. Penelitian dilakukan dengan metode observasi lapang, wawancara, dan studi pustaka. Hasil penelitian menunjukkan bahwa pemeliharaan elang jawa di TSI Bogor telah terlaksana cukup baik. Pemanfaatan elang jawa berupa wisata edukasi, pelepasliaran, dan sarana penelitian. Nilai persepsi pengunjung terkait pelestarian elang jawa diperoleh sebesar 82,6 % dengan preferensi pengunjung menjadikan elang jawa sebagai satwa obyek wisata prioritas urutan ke-2. Nilai ekonomi elang jawa berdasarkan biaya pemeliharaan sebesar Rp7.097.200 /ekor/bulan, nilai rata-rata WTP untuk mendukung upaya konservasi elang jawa sebesar Rp94.850 dengan nilai ekonomi total perbulan sebesar Rp6.029.140.250, dan nilai rata-rata WTP untuk pengembangan pemanfaatan elang jawa sebesar Rp70.005 dengan nilai ekonomi total perbulan sebesar Rp4.449.867.825., The Javan eagle is a protected animal by the Indonesian Government and IUCN in the Endangered category (IUCN 2016). The Javan eagle continues to be threatened, so ex-situ conservation efforts are needed, one of which is being carried out at Taman Safari Indonesia (TSI) Bogor. This research aims to identify maintenance management, utilization and economic value of Javan eagles at TSI Bogor. The research was conducted using field observation, interviews and literature study methods. The results of the research show that the maintenance of Javan eagles at TSI Bogor has been carried out quite well. Javanese eagles are used in the form of educational tourism, releases and research facilities. The visitor perception value was obtained at 82.6 % with preferences including the 2nd priority. The economic value of the Javan eagle based on maintenance costs is IDR 7,097,200 / head / month, theaverage WTP value to support Javanese eagle conservation efforts is IDR 94,850 with a total monthly economic value of IDR 6,029,140,250, and the average WTP value for development of the use of Javanese eagles amounting to IDR 70,005 with a total monthly economic value of IDR 4,449,867,825.
Keyword: Nilai ekonomi, Pemanfaatan, Pemeliharaan