Judul: Transplantasi sel testikular ikan gurame Osphronemus gouramy pada ikan nila Oreochromis niloticus umur 1-4 hari
Abstrak: Giant gouramy is one of the targeted farmed species to increase its production level in the Indonesian Ministry of Maritime Affairs and Fisheries Program 2010-2014. Its production seemed slow because of the relatively long reproductive cycle. Transplantation of giant gouramy testicular cells to tilapia can be done in the hope that the surrogate broodstock (tilapia) will be able to give birth giant gouramy so the production cycle can be accelerated. In this research, transplantation of giant gouramy testicular cell (donor) in the peritoneal cavity of newly hatched tilapia (recipient) was performed. Giant gouramy testicular cells that contain spermatogonial cells obtained by dissociation of the gonad using PBS with 0.5% trypsin and then injected into tilapia with different ages. The results showed that SR (survival rate) of 1-2 days old injected larvae (A; 89.34%) were lower compared to that of 3-4 days old (B; 98.96%). Furthermore, the success rate of donor cells colonization in the recipient gonad at 2 months after injection in treatment A (6 of 6 recipients; 100%) was higher than that of treatment B (3 of 4 recipients; 75%). The results of this study indicated that transplantation using 1-2 days old larvae gave highest colonization and comparable level of SR. Thus, this technique has the potential both to manipulate gamet production of economically important fish species that relatively difficult to reproduction (commercial) and reproduce the endangered fish (as conservation).
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Judul: Transplantasi sel testikular ikan neon tetra Paracheirodon innesi pada benih ikan mas umur berbeda
Abstrak: Ikan neon tetra Paracheirodon innesi merupakan ikan hias yang memiliki nilai ekspor yang tinggi. Namun demikian, tingkat produksinya masih relatif rendah karena fekunditas ikan neon tetra yang sedikit (sekitar 180 telur/induk). Teknologi transplantasi sel testikular ikan neon tetra (ikan donor) ke ikan mas yang memiliki fekunditas telur yang banyak dan diharapkan mampu mengatasi ketersediaan benih ikan neon tetra. Penelitian ini bertujuan untuk menentukan umur optimum benih ikan mas (calon ikan semang) yang mampu menerima sel target dengan baik dan memiliki keberhasilan kolonisasi yang tinggi. Testis ikan neon tetra didisosiasi menggunakan larutan tripsin 0,5%. Sel testikular diwarnai dengan PKH-26, kemudian ditransplantasikan ke rongga peritoneal benih ikan mas umur 7, 10 dan 14 hari setelah menetas. Hasil penelitian menunjukkan bahwa tingkat kelangsungan hidup ikan mas perlakuan transplantasi umur 7 hari (31,25%) lebih rendah dibandingkan dengan perlakuan transplantasi umur 10 hari (37,50%) dan 14 hari (56,25%). Persentase ikan terkolonisasi sel donor pada hari ke-21 pascatranplantasi pada benih umur 7 dan 10 hari adalah sama (80%), sedangkan transplantasi benih umur 14 hari sebesar 60%. Berdasarkan keberhasilan transplantasi secara kumulatif (tingkat kelangsungan hidup dan kolonisasi), transplantasi pada benih umur 14 hari (33,75%) menunjukkan hasil lebih tinggi dibandingkan dengan perlakuan transplantasi pada benih umur 7 hari (25,00%) dan benih umur 10 hari (30,00%). Dengan demikian, transplantasi sel testikular ikan neon tetra pada benih ikan mas telah berhasil dilakukan, dan umur optimum benih ikan mas adalah 14 hari setelah menetas.
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Judul: The Role of Leaders in Collective Action for the Poor Urban Communities during the Covid-19 Pandemic (Case: An RW in West Bogor District, Bogor City, West Java)
Abstrak: Wabah pandemi Covid-19 sebagai bencana non alam menjadi faktor risiko bagi masyarakat terlebih bagi komunitas miskin. Upaya untuk mencegah serta menanggulangi pandemi Covid-19 ini perlu didukung dengan aksi bersama komunitas serta didorong oleh kepemimpinan yang kuat. Peranan kepemimpinan dalam mengelola bencana selama dan setelah situasi krisis dapat mendorong komunitas berperan aktif dan tangguh di masa pandemi melalui penguatan aksi kolektif. Penelitian ini menganalisis peran pemimpin pada aksi kolektif komunitas miskin perkotaan di masa pandemi Covid-19. Pengumpulan data dilakukan dengan metode survei pada 60 responden yang diperkuat dengan wawancara mendalam. Hasil penelitian menunjukkan terdapat hubungan signifikan antara peran pemimpin dengan tingginya tingkat aksi kolektif pada komunitas miskin perkotaan selama Pandemi Covid-19. Pemimpin yang dinilai mampu menggerakkan warga komunitas untuk berpartisipasi aktif pada setiap aksi kolektif selama masa pandemi Covid-19 adalah tokoh Ketua RW, The Covid-19 pandemic as a non-natural disaster is a risk factor for the community, especially for the poor. Efforts to prevent and overcome the Covid-19 pandemic need to be supported by joint community action and strong leadership. The leadership role in managing disasters during and after crisis situations can encourage active and resilient communities during a pandemic through supportive collective action. This study analyzes the role of leaders in the collective action of urban poor communities during the Covid-19 pandemic. Data was collected using a survey method on 60 respondents who conducted interviews. The results showed that the relationship between the role of leaders and the level of collective action in urban poor communities was significant during the Covid-19 pandemic. The leader who is considered capable of mobilizing the community to actively participate in every joint action during the Covid-19 pandemic is the leader of the RW
Keyword: collective action, poor communities, the role of leaders