The arterial blood was withdrawn before TAES ( T@ ) , before anesthesia ( T@ ) , before cutting ( T@ ) , at @ min after encephalic incision ( T@ ) , immediately after incisions suture ( T@ ) , at about @ min after removing tracheal catheters ( T@ ) to detect beta-endorphin ( beta-EP ) , cortisol ( COR ) , adrenalin ( E ) , blood sugar ( Glu ) .