At baseline and @ hours after treatment , we assessed ( @ ) flow-mediated dilation of brachial artery ; ( @ ) coronary flow reserve , ejection fraction , systemic arterial compliance , and resistance by echocardiography ; ( @ ) left ventricular global longitudinal and circumferential strain , peak twisting , untwisting velocity by speckle tracking ; and ( @ ) interleukin-@ , nitrotyrosine , malondialdehyde , protein carbonyl , and Fas/Fas ligand levels .