TITLE: Nutritional Status of Vitamin D and the Effect of Vitamin D Supplementation in Korean Breast-fed Infants

  ABSTRACT:
We investigated the vitamin D status and the effect of vitamin D supplementation in Korean breast-fed infants. The healthy term newborns were divided into 3 groups; A, formula-fed; B, breast-fed only; S, breast-fed with vitamin D supplementation. We measured serum concentrations of vitamin D (25OHD3), calcium (Ca), phosphorus (P), alkaline phosphatase (AP), intact parathyroid hormone (iPTH) and bone mineral density (BMD) at 6 and 12 months of age. Using questionnaires, average duration of sun-light exposure and dietary intake of vitamin D, Ca and P were obtained. At 6 and 12 months of age, 25OHD3 was significantly higher in group S than in group B (P<0.001). iPTH was significantly lower in group S than in group B at 6 months (P=0.001), but did not differ at 12 months. Regardless of vitamin D supplementation, BMD was lower in group B and S than in group A (P<0.05). Total intake of vitamin D differed among 3 groups (P<0.001, A>S>B), but total intake of Ca and P were higher in group A than in group B and S (P<0.001). In conclusion, breast-fed infants show lower vitamin D status and bone mineralization than formula-fed infants. Vitamin D supplementation (200 IU/day) in breast-fed infants increases serum 25-OH vitamin D3, but not bone mineral density.

BODY.INTRODUCTION:
Vitamin D, a fat-soluble steroid hormone, is important for calcium and phosphorus homeostasis and musculoskeletal health. In children, severe vitamin D deficiency manifests as rickets, and vitamin D inadequacy can impair attainment of peak bone mass. In adults, inadequate vitamin D can result in secondary hyperparathyroidism, decreased bone mineral density, osteomalacia, and increased risk of fragility fractures (1). Moreover, inadequate vitamin D may be involved in the pathogenesis and/or progression of several disorders, including cancer, hypertension, osteoarthritis, diabetes, and other autoimmune diseases (1-6).  Infants between 4 months and 2 yr of age are at a high risk for the relative vitamin D deficiency because of their rapid growth in skeletal system. The incidence of vitamin D deficiency during infancy had been decreased in the past with the improvement of nutritional condition such as vitamin D supplementation in infant formula. But, lately it has been increased again for various reasons such as breast-feeding from vitamin D deficient mother, lack of sun-light exposure (cosmetic or dermatologic reasons, sun-block, etc.), high latitude, dark skin, and low dietary intake of vitamin D, etc. (7-10). In response to the resurgence of rickets especially in breast-fed infants, the American Academy of Pediatrics revised its policy on vitamin D supplementation to infants and children in 2003 (11).  The incidence of vitamin D deficiency would be high as well in Korean infants for similar reasons, but there are few clinical studies performed to evaluate the nutritional status of vitamin D during infancy. Therefore, in this study, we evaluate the vitamin D status in Korean infants, and further determine the efficacy of vitamin D supplementation on the vitamin D nutritional status and bone mineralization in term breast-fed infants.

BODY.MATERIALS AND METHODS.SUBJECTS AND STUDY DESIGN:
This study was approved by the Institutional Review Board of Chungbuk National University. And the informed consents were obtained from mothers. The healthy term infants delivered at Chungbuk National University Hospital from July 2004 through June 2005 were enrolled in this study. Study infants were divided into 3 groups: A, formula-fed; B, breast-fed only without supplementation; S, breast-fed with vitamin D supplementation. During 1 yr follow-up period after enrollment, serum concentrations of vitamin D (25OHD3), calcium (Ca), inorganic phosphorus (P), alkaline phosphatase (AP), intact parathyroid hormone (iPTH), bone mineral density (BMD), and bone mineral content (BMC) were measured at birth, 6 and 12 months of age. Average duration of sunlight exposure (min/day) and total intake of vitamin D, Ca and P were obtained by maternal interview using questionnaires.

BODY.MATERIALS AND METHODS.VITAMIN D SUPPLEMENTATION:
In group S infants, 0.5 mL of Poly-Vitamin Drops® (Hi-Tech Pharmacal Co. Inc., Amityville, NY, USA) was given once a day from 2 months of age till the end of this study. Poly-Vitamin Drops®, a multivitamin preparation, contains vitamin A (1,500 IU/mL), vitamin D (400 IU/mL), vitamin E (5 IU/mL), vitamin C (35 mg/mL), thiamin (0.5 mg/mL), riboflavin (0.6 mg/mL), niacin (8 mg/mL), vitamin B6 (0.4 mg/mL), iron (10 mg/mL), and fluoride (0.25 mg/mL).

BODY.MATERIALS AND METHODS.BIOCHEMICAL ANALYSIS:
Serum samples were stored at -20°C in light-proof bottles before biochemical assay in the Green-Cross Laboratory, Seoul, Korea. Serum 25OHD3 concentration was measured by radioimmunoassay (25OH-VIT.D3-RIA-CA kit, Biosource, Europe; intra-assay and interassay coefficient of variation, 4.7 and 5.3, respectively) and intact PTH (iPTH) by electrochemiluminescence (reference: 15-65 pg/dL).  In this study, vitamin D deficiency, insufficiency, and sufficiency were defined as serum 25OHD3 level less than 11 ng/mL, 11-30 ng/mL, and over 30 ng/mL, respectively (1, 4, 12, 13).

BODY.MATERIALS AND METHODS.ANALYSIS OF BONE MINERALIZATION:
Study infants at 6 and 12 months of age underwent the measurement of lumbar-spine (L2-L4) bone mineral content (BMC, g) and areal bone mineral density (BMD, g/cm2) by dual-energy radiography absorptiometry (DXA) using the Lunar Expert-XL DEX system (Lunar Corp., Madison, WI, USA) or Norland XR-36 system (Norland Medical System, Inc., Atkinson, WI, USA) according to the manufacturer's operator manuals.

BODY.MATERIALS AND METHODS.SURVEY: SUN-LIGHT EXPOSURE AND DIETARY INTAKE:
The average duration of sun-light exposure per day (UV, min/day) were derived from the minutes spent outside during 3 days preceeding the visit. Dietary intake data were collected using a 24-hr recall method at 6 and 12 months of age to estimate the intakes of vitamin D, calcium, and phosphorus. Breast milk intake was estimated by multiplying the nursing frequency and the average amount of milk per nursing which was measured in 6 month-old infants in a previous study (14). The intake of each nutrient was calculated based on the nutrient database in the 8th edition of Pediatrics (vitamin D, 6 μg/L; calcium, 270 mg/L; phosphorus, 140 mg/L of breast milk), the 7th Korean Recommended Dietary Allowances (15), vitamin D composition published in Korea Health Industry Development Institute (16), and the nutrition label claimed by the manufacturers in formula using DS24 WIN Program (nutrient analysis software developed by Human Nutrition Lab. in Seoul National University) in the Department of Food and Nutrition.

BODY.MATERIALS AND METHODS.STATISTICAL ANALYSIS:
We assessed differences between groups using chi-square test for categorical variables and t-test or ANOVA followed by Duncan procedure for post-hoc test for continuous variables, as appropriate. P<0.05 was considered statistically significant.

BODY.RESULTS.DEMOGRAPHIC DATA, LIFESTYLE CHARACTERISTICS, AND VITAMIN D STATUS OF THE STUDY INFANTS AND MOTHERS AT BIRTH:
Total 74 newborns and their mothers were studied at birth, among which 25, 28, and 21 infants belonged to the group A (formula-fed), group B (breast-fed only), and group S (breast-fed with vitamin D supplementation), respectively.  About 60% of study infants were male, and their mean gestational age, body weight, length, and head circumference at birth were 276±9.0 days, 3.25±0.37 kg, 49.7±2.01 cm, and 34.0±1.37 cm, respectively. There were no differences among 3 study groups in demographic and lifestyle characteristics such as maternal age, job, education and residence at the time of delivery. Study infants were enrolled for 1 yr spanning all 4 seasons, with 48-62% delivered from June through November (Table 1).  The mean 25OHD3 level was 24.5±10.7 ng/mL in mothers, and 17.7±7.0 ng/mL in newborns, of which 18% were vitamin D deficient and 73.6% were insufficient. There were no statistically significant differences among our 3 study groups in laboratory data at birth as well (Table 2).

BODY.RESULTS.VITAMIN D STATUS AND THE EFFECT OF VITAMIN D SUPPLEMENTATION IN BREAST-FED INFANTS AT 6 MONTHS OF AGE:
Number of study infants at 6 months were 25, 28, and 21 in group A, B, and S, respectively. The mean age was 179±12.0 days, and there were no differences among 3 groups in infants' age, sex, and the other anthropometric data (Table 3).  The mean 25OHD3 levels of infants in group A, B, and S were 44.0±11.6, 22.5±9.24, and 34.7±12.3 ng/mL, respectively, which were significantly different among 3 groups (P<0.001, A>S>B). The prevalence of vitamin D deficiency and insufficiency were 0% and 12%, 11% and 68%, and 0% and 45% in group A, B, and S, respectively. Serum Ca and AP levels were not different (P>0.05), but serum P level was significantly different among 3 groups (P<0.001, A>S>B). The iPTH levels in group B was 45.1±46.3 pg/mL, significantly higher than those in group A (15.8±5.48 pg/mL) and S (13.9±6.42 pg/mL) (P=0.001, B>A=S). Especially, all 6 infants whose 25OHD3 levels were less than 15 ng/mL had the iPTH levels above 40 ng/mL, presenting secondary hyperparathyroidism due to vitamin D deficiency (Table 3, Fig. 1A, B, 2A).  The BMD at 6 months of age were 0.278±0.045, 0.247±0.053 and 0.233±0.054 g/cm2 in group A, B, and S, respectively. Both BMD and BMC were lower in group B and S than those in group A (P<0.05, A>B=S) (Table 3, Fig. 1C).

BODY.RESULTS.VITAMIN D STATUS OF STUDY INFANTS AND THE EFFECT OF VITAMIN D SUPPLEMENTATION IN BREAST-FED INFANTS AT 12 MONTHS OF AGE:
Number of infants participated in 12 months evaluation were 30, 16, and 15 in group A, B and S, respectively. The mean age was 370±13.2 days, and there were no differences among 3 groups in infant age, sex, and other anthropometric data (P>0.05), except the length (P=0.006, A>B, Table 4).  The 25OHD3 levels of infants in group A, B, and S were 48.8±9.74, 33.2±7.89 and 42.8±9.80 ng/mL, respectively, which was the lowest in group B and showed no differences between group A and S (P<0.001, A=S>B). None of the study infants at 12 months were vitamin D deficient, although 50% of infants in group B were vitamin D insufficient. Serum Ca and AP levels were not different among 3 groups, whereas serum P was significantly lower in group S than in group A (P=0.009, A>S, A=B, and B=S). The iPTH levels in group A, B, and S were 18.0±6.68, 22.8±7.04, 24.9±9.62 pg/mL, respectively. Group S showed significantly higher iPTH than group A, although neither group A nor group S was significantly different from group B (P=0.025, S>A, A=B, and B=S) (Table 4, Fig. 1A, B).  The BMDs in group A, B, and S at 12 months of age were 0.326±0.058, 0.286±0.078, and 0.282±0.037 g/cm2, respectively, which was significantly higher in group A than those in both group B and S (P=0.010, A>B=S). The BMC was significantly higher in group A than in group S, but there were no differences either between group A and B or between group B and S (P=0.025, A>S, A=B, and B=S) (Table 4, Fig. 1C).

BODY.RESULTS.DIETARY INTAKE OF VITAMIN D AND SUN-LIGHT EXPOSURE AT 6 AND 12 MONTHS OF AGE:
The mean amount of formula intake per day in group A were 874±207, 806±208 mL/day at 6 and 12 months of age, whereas the estimated daily amount of breast-milk intake in group B and S were 793±178 and 788±157 mL/day at 6 months of age, 488±164 and 459±236 mL/day at 12 months of age. The ages when solid food was first introduced were similar among 3 groups, 5.32±1.12, 5.79±0.99, 5.76±0.83 months in group A, B, and S, respectively.  At both 6 and 12 months of age, total intake of vitamin D (the sum of vitamin D intake from milk, solid food and vitamin supplementation) were the highest in group A, middle in group S, and lowest in group B (P<0.001, A>S>B). Total intake of Ca and P were higher in formula-fed infants than breast-fed infants (P<0.001, A>B=S). In the mean time, average durations of sun-light exposure were similar in 3 groups (Table 3, 4).

BODY.DISCUSSION:
In this study, serum levels of 25-OH vitamin D3 (25OHD3), iPTH and BMD were measured as indices for vitamin D nutritional status (3, 12, 17). In general, vitamin D deficiency in infancy and childhood presents clinical manifestations such as rickets, and vitamin D insufficiency may result in an asymptomatic biochemical abnormality such as secondary hyperparathyroidism (5, 13, 17, 18). We defined vitamin D deficiency, insufficiency, and sufficiency as 25OHD3 less than 11 ng/mL, 11-30 ng/mL, and over 30 ng/mL, respectively (4, 12, 13). However, vitamin D deficient infants as defined in our study did not show clinical symptom of rickets. Only 6 infants whose 25OHD3 levels less than 15 ng/mL showed increased iPTH levels above 40 pg/mL, suggestive of secondary hyperparathyroidism due to vitamin D insufficiency.  In adults, BMD correlates with the serum vitamin D levels (19), whereas, in infants, it has been reported to have no correlation with the serum vitamin D levels (20-23). Since lumbar-spine (L2-L4) bone mineral density does not have normal reference values for the infants less than 1 yr of age, we simply compared BMDs among 3 study groups.  The overall prevalence of vitamin D deficiency and insufficiency in our study were 18% and 74% at birth, 4% and 43% at 6 months, and 0% and 15% at 12 months of age. The prevalence of vitamin D deficiency in our study is lower than the data reported by Challa et al. (56-91% at birth and 21-30% at 6 months), but similar to the data by Weiler et al. (36% at birth by using cord blood levels of vitamin D less than 11 ng/mL) (20, 24).  Serum vitamin D level was determined by 2 major factors, i.e., the dietary intake and production in the skin when exposed to the sun (ultraviolet B irradiation, UVB) (1). Healthy term infants are born with the storage of vitamin D enough for next couple of months, and the half-life of serum vitamin D is 12-20 days. Infant formula has been fortified with sufficient amount of vitamin D (>400 IU/L), whereas breast milk contain only 12-60 IU/L of vitamin D which is much less than the required maintenance amount (200 IU/day) as recommended by National Academy of Science (NAS) (25, 26). On top of that, dermal conversion is seasonally unreliable source of vitamin D and may not compensate for low nutritional intake, particularly in infants whose skin exposure to sunlight is limited (27). Therefore, exclusively breast-fed infants are at high risk of vitamin D deficiency and insufficiency. Actually, the breast-fed infants showed significantly lower vitamin D status and bone mineralization than the formula-fed infants, as revealed in this study.  Measures to prevent vitamin D deficiency include increased skin exposure to sunlight, increased fortification of food items with vitamin D, and vitamin D supplementation (13). In our study, we supplemented breast-fed infants with 200 IU/day of vitamin D from 2 months of age as recommended by American Academy of Pediatrics (AAP) in 2003 in order to evaluate the efficacy of vitamin D supplementation. We compared their vitamin D status and BMD with those of 2 control groups, one with breast-fed without supplementation and the other with formula-fed at 6 and 12 months of age. Our results showed that vitamin D supplementation (200 IU/day) in breast-fed infants increased serum 25-OH vitamin D3, but did not increase bone mineral density. As a matter of fact, BMD and BMC were lower in breast-fed infants than in formula-fed infants, regardless of vitamin D supplementation. As vitamin D level increased at 6 months, iPTH in supplemented group was significantly lower than in breast-fed control. But at 12 months of age, unlike our expectation, iPTH in supplemented group became rather higher, yet of no significant difference, despite higher vitamin D level as compared with breast-fed control.  Season, residence, and sun-light exposure were compared in order to evaluate the factors on vitamin D status in this study, and there were no differences among 3 study groups. On the contrary, dietary intake of vitamin D, Ca, and P at 6 and 12 months were significantly different among 3 groups. According to our survey data, daily intake of these nutrients in all breast-fed infants, regardless of vitamin supplementation, were lower than those recommended for 5-11 month-old infants in the 7th Korean Recommended Dietary Allowances (10 μg/day of vitamin D, 300 mg/day of calcium, and 300 mg/ day of phosphorus).  Based on our results, it was supposed that 200 IU/day of vitamin D supplementation in breast-fed infants did increase serum 25-OH vitamin D3, but did not increase BMD, and did not fully correct iPTH at 12 months. Regarding these unexpected results in our study, we speculated firstly that 200 IU/day of vitamin D supplementation in AAP recommendation (2003) might not be enough to correct the vitamin D insufficiency in late infancy. Since some researchers have suggested 400-800 IU/day of vitamin D intake for the prevention of vitamin D deficiency, further studies are warranted to examine dose-responsive relationship of vitamin D supplementation (1, 9, 28).  Secondly, the amount of calcium intake should be considered. BMD depends mainly on the amount of total body calcium. Therefore, an increase in calcium intake alone can enhance bone mineralization and effectively suppress the secretion of iPTH (5, 13). It is plausible that the difference in BMD and iPTH between the breast-fed and the formula-fed group might result from a large amount of calcium intake in formula-fed infants, independent of vitamin D. In addition, 1,25(OH)2D could mobilize calcium by increasing bone resorption of osteoclasts if the calcium intake is very low (1, 5). The unexpected result of iPTH in supplemented group at 12 months might also be caused by a relative excess of vitamin D to low Ca, P intake in breast-fed infants (29). Therefore, a clinical trial of co-supplementation of vitamin D with calcium would be considered to elucidate this possibility.  Lastly, the other nutrients such as iron (10 mg/mL) and fluoride (0.25 mg/mL) contained in our multivitamin preparation for vitamin D source should be elucidated, because those nutrients could influence vitamin D status and bone mineralization. Currently there is no commercially available preparation of vitamin D alone. A preparation of vitamin D alone needs to be developed and used in future study to exclude this problem.  In conclusion, vitamin D deficiency or insufficiency was relatively common in Korean infants. Especially, breast-fed infants showed lower vitamin D status and bone mineralization than formula-fed infants. Vitamin D supplementation (200 IU/day) in breast-fed infants increased serum 25-OH vitamin D3, but did not increase bone mineral density.

Question: Compared to Breast milk alone what was the result of Formula on Bone mineral density?
Evidence: 25OHD3 was significantly higher in group S than in group B (P<0.001)
Answer: significantly increased