TITLE: Tai Chi Chuan and Baduanjin practice modulates functional connectivity of the cognitive control network in older adults

  ABSTRACT:
Cognitive impairment is one of the most common problem saffecting older adults. In this study, we investigated whether Tai Chi Chuan and Baduanjin practice can modulate mental control functionand the resting state functional connectivity (rsFC) of the cognitive control network in older adults. Participants in the two exercise groups practiced either Tai Chi Chuan or Baduanjin for 12 weeks, and those in the control group received basic health education. Memory tests and fMRI scans were conducted at baseline and at the end of the study. Seed-based (bilateral dorsolateral prefrontal cortex, DLPFC) rsFC analysis was performed. We found that compared to the controls, 1) both Tai Chi Chuan and Baduanjin groups demonstrated significant improvements in mental control function; 2) the Tai Chi Chuan group showed a significant decrease in rsFC between the DLPFC and the left superior frontal gyrus (SFG) and anterior cingulate cortex; and 3) the Baduanjin group showed a significant decrease in rsFC between the DLPFC and the left putamen and insula. Mental control improvement was negatively associated with rsFC DLPFC-putamen changes across all subjects. These findings demonstrate the potential of Tai Chi Chuan and Baduanjin exercises in preventing cognitive decline.

BODY:
Cognitive impairment is a common problem affecting older adults. It decreases quality of life and increases disability and healthcare costs. Accumulating evidence suggests that physical activity or mental training practices may prevent age-related cognitive impairment12. Tai Chi Chuan is a mind-body exercise, which was originally developed as a martial art in China. It has been practiced for many centuries and is gaining popularity in the West. According to the 2007 National Health Interview Survey, 2.3 million U.S. adults had practiced Tai Chi Chuan in the past 12 months3. As a complex, multicomponent intervention, Tai Chi Chuan combines coordination of slow movements with mental focus, deep breathing, and relaxation. A recent meta-analysis of twenty eligible studies with a total of 2,553 participants showed that Tai Chi Chuan practice can enhance cognitive functioning in older adults4.  Baduanjin is another popular traditional Chinese mind-body exercise that focuses on breathing, increasing flexibility, and strengthening muscles and tendons. Compared to Tai Chi Chuan, Baduanjin is a simpler practice that involves eight fixed movements that can be learned easily, and is also less demanding physically and cognitively. Previous studies showed that both Tai Chi Chuanand Baduanjin can improve cognitive functioning56. However, the underlying mechanisms of Tai Chi Chuan and Baduanjin remain unclear.  Studies suggest that human aging is associated with cognitive impairment and altered brain activation patterns on a number of cognitive tasks7. Recently, resting state functional connectivity (rsFC) has been applied to investigate the pathology of brain networks in cognitive impairment in older adults. One such brain network is the cognitive control network (CCN), which is involved in cognitive control processes8. As for memory encoding, cognitive control processes can not only encode and store relevant information, but also suppress irrelevant information encoding9.  The CCN includes the frontoparietal brain regions1011 and is key in top-down modulation of attention–memory interactions812. A large body of evidence indicates that the dorsolateral prefrontal cortex (DLPFC) is a key region of the CCN, playing an important role in cognitive control processes1314. Previous studies showed that non invasive stimulation of the left DLPFC using anodal transcranial directcurrent stimulation (tDCS) canreduce memory loss in older adults with episodic memory impairment and strengthen existing memories15, underlining its role in memory function.  In this study, we investigated the CCN rsFC and mental control function (a subtest in the Wechsler Memory Scale, WMS) changes after 12 weeks of Tai Chi Chuan or Baduanjin practice in older adults. We choose mental control subtest in this study because we believe the task can better reflect the function of the CCN than the general score of Wechsler Memory Scale. We hypothesized that 12-week Tai Chi Chuan or Baduanjin practice could significantly modulate the functional connectivity of the CCN and improve mental control functionin older adults.

BODY.RESULTS:
One hundred and two subjects were screened, of which 90 subjects passed screening and completed baseline scans. Sixty-two subjects (21 in the Tai Chi Chuan group, 16 in the Baduanjin group, and 25 in the control group) completed all study procedures and fMRI scans. Four subjects dropped out from the Tai Chi Chuan group (1 due to relocation out of the city, 1 due to inability to get an MRI scan, 2 due to scheduling conflicts). Nine subjects dropped out from the Baduanjin group (8 due to scheduling conflicts, 1 due to unwillingness to participate in the MRI scan). Fifteen subjects dropped out from the control group (11 due to scheduling conflicts, 4 due to inability to participate in post-treatment MRI scans). In addition, we also dropped one subject from the Baduanjin group during data analysis due to significant head motion (exceeded 3.0 mm) Table 1 follows.

BODY.RESULTS.CLINICAL OUTCOMES:
The demographics of the three groups of subjects are shown in Table 1. There were no significant differences between the three groups in age, gender, handedness, average years of education, and the mental control score at the beginning of the treatment (P > 0.05). The average attendance rate in the Tai Chi Chuan group was 95%, ranging from 88% to 100%, while in the Baduanjin group it was 98%, ranging from 92% to 100%.  After 12-week interventions, the mental control subscores were 34.52 ± 3.17, 36.00 ± 3.78, 28.46 ± 5.76 (mean ± SD) for Tai Chi Chuan, Baduanjin and Control respectively. Mixed-model regression analysis showed significant mental control score increases in the Tai Chi Chuan and Baduanjin groups compared with the control group (Baduanjin: p < 0.001, Tai Chi Chuan: p = 0.017). No significant differences were found between the Tai Chi Chuan and Baduanjin groups (p = 0.116).

BODY.RESULTS.RESTING STATE FUNCTIONAL CONNECTIVITY RESULTS:
The results of resting state functional connectivity analysis are presented in Table 2 and Fig. 1. After the 12-week practice, we found significant CCN rsFC decreases in the left superior frontal gyrus, left dorsal anterior cingulate cortex (dACC), and rostral anterior cingulate cortex (rACC) in Tai Chi Chuan subjects compared with controls (Fig. 1A,B). Subjects in the Baduanjin group showed a significant CCN rsFC decrease in the left putamen/insula compared with subjects in the control group (Fig. 1C). No significant functional connectivity differences were observed between theTai Chi Chuan and Baduanjin groups.  Regression analysis between mental control function and CCN rsFC across all subjects showed a negative association with the left putamen (Table 3, Fig. 1C). The results overlapped with areas of significant rsFC decrease in the Baduanjin group as compared to the control group (Fig. 1C).  In a previous study16 using the same dataset, we investigated the resting state functional connectivity of the hippocampus and found significant rsFC increases between the hippocampus and the medial prefrontal cortex (mPFC) after Tai Chi Chuan practice (we chose Tai Chi Chuan practice because we only observed significant differences when comparing Tai Chi Chuan with control groups). To explore the modulation effects of Tai Chi Chuan on the CCN and the hippocampal network, we extracted the Fisher z values (with 3 mm sphere) of all significant findings and applied regression analysis between the significant rsFC changes observed in the two studies. We found no significant associations between the CCN and hippocampus rsFC changes observed in the two studies after Tai Chi Chuan practice.

BODY.DISCUSSION:
In this study, we found that mental control function significantly increased in both Tai Chi Chuan and Baduanjin groups compared with the control group after 12 weeks of practice. We also found that 12-week Tai Chi Chuan practice significantly decreased bilateral DLPFC rsFC with the left superior frontal gyrus and ACC, while longitudinal Baduanjin practice showed significant decreases in bilateral DLPFC rsFC with the left putamen and insula compared with subjects in the control group. The rsFC between the DLPFC and the left putamen was significantly negatively associated with mental control scores across all subjects.  A previous study showed that the CCN is preferentially activated in memory-guided visual attention processing12. Studies on the CCN and aging found that reduced functional connectivity within the fronto-parietal network in older adults may be consistent with the expected increase in misguided attention17. Neuroimaging data also suggested that high-performing older adults may compensate for disruption of the cognitive control network by recruiting additional frontal resources to overcome cognitive control deficits that affect recognition memory18.  On the other hand, studies also reported that while greater connectivity of the right DLPFC predicts better memory performance, overall increased activity in the task-positive network areas requiring greater demand of cognitive control processes predicts poorer accuracy on attention and memory tasks in older adults compared to younger adults19. Therefore, the benefits associated with increased activity within the CCN might be related to compensatory mechanisms rather than indexing a healthy state, whichsuggestshyperactivation of the CCN is not overall desirable. For example, a study that investigated the effect of a 14-day longevity lifestyle program, which included mental and physical exercise, stress reduction, and a healthy diet, found that improved memory and brain metabolism were associated with a decrease in the connectivity of the left DLPFC, which the authors interpreted as a marker of greater cognitive efficiency of this brain region involved in working memory20.  The positive effect of physical exercise on memory has been reported in a number of studies2122. However, the results of the studies investigating the effect of physical exercise on the regions of the CCN have been somewhat heterogeneous. On the one hand, it has been shown that acute moderate exercise elicits enhanced dorsolateral prefrontal activation and improves Stroop task performance23. On the other hand, several studies reported hypofrontality during exercise and related it to exercise-induced enhancement of cognition and emotion24. In a study that tested the effect of different exercise protocols (endurance or strength oriented) on brain activity and cognitive performance, the authors found a decrease in prefrontal cortex activation following endurance exercise and a related improvement in cognitive performance, suggesting that general defocusing caused by exercise acts as a cognitive enhancer25.  In this study, we found that Tai Chi Chuan and Baduanjin exercises reduced rsFC in the CCN compared to the control group. This reduction was also associated with improvement in memory function. Together, these findings suggest that longitudinal exercise treatment might enhance cognitive performance while decreasing CCN connectivity, possibly indexing increased efficiency of the cognitive control system and eliminating the need for compensatory hyperactivation of the network.

BODY.DISCUSSION.EFFECTS OF TAI CHI CHUAN PRACTICE:
We found that Tai Chi Chuan practice significantly reduced the rsFC between the DLPFC and ACC compared with the controls. Studies have suggested that there are two necessary components during the regulation of cognition: one in which the DLPFC plays an important role in implementing control, and another in which the ACC monitors performance and signals when adjustments in control are needed26. Similarly, neuroimaging studies have suggested that the ACC and DLPFC may exert different cognitive controls on human memory processes2728. The role of the DLPFC involves executive control during psychological processes, active maintenance of memory, and associative memory, while the ACC is in control of memory-relevant attention, conflict monitoring, and in formation filtering and extraction272930.  Previous studies on meditation showed that the ACC plays an important role in improving cognitive function during a short-term meditative state31. For instance, a study found that compared to controls and short-term practitioners, long-term practitioners of meditation showed significantly more consistent and sustained activation in the DLPFC and ACC during meditation32. Using the surface-based regional homogeneity (ReHo) method, Wei and colleagues33 found that Tai Chi Chuan experts showed significant decreases in ReHo at the left ACC and the right superior frontal cortices compared to control subjects. Our results are consistent with these findings. Since Tai Chi Chuan practice incorporates elements of meditation, we observed significant modulation of the DLPFC connectivity with the ACC.  Furthermore, we found that Tai Chi Chuan practice can significantly reduce the rsFC between the DLPFC and superior frontal gyrus. Numerous human studies have reported the important role of the superior frontal gyrus in cognitive functions, including working memory3435. Neuroimaging studies found that the superior frontal gyrus is part of a network for voluntary attentional control36. Previous studies also demonstrated that aging affects activity levels in this region37. Namely, when compared with older adults, young adults showed greater activity in the bilateral superior frontal gyrus38.  Similarly to the ACC, investigators also found that the frontal gyrus is implicated in meditation and the magnitude of its engagement is dependent on experience. For example, meditation experts with at least 3 years of experience had lower sustained activation in attention-related brain areas, including the left SFG compared with control subjects39. Multimodal intervention has also been found to have an effect on the superior frontal gyrus. In one study, cognitive training, Tai Chi Chuan exercise, and group counseling enhanced the amplitude of low frequency fluctuations (ALFF) in the superior frontal gyrusin older adults40. Our results provide additional support for the positive effects of Tai Chi Chuan on the superior frontal gyrus through increased efficiency in cognitive control, which are comparable to the effects evoked by meditation.

BODY.DISCUSSION.EFFECTS OF BADUANJIN PRACTICE:
We found significant decreases between the bilateral DLPFC and the left putameninthe Baduanjin group compared with subjects in the control group. The putamen is a key region in the basal ganglia41. The basal ganglia has traditionally been thought of as a motor processing nuclei; however, recent studies have implicated the basal ganglia in complex cognitive tasks42. Previous studies suggested that both the PFC and basal ganglia contribute to working memory4344. Baierand colleagues found that the basal ganglia plays the role of gatekeeper, only allowing relevant information to enter the prefrontal cortex where the information is actively maintained in working memory43. Researchers also found that coordinative exercise, a favorable leisure activity, has the potential to improve the volume of the basal ganglia in older adults45. Our results are consistent with findings from the above studies, indicating the important roles of the DLPFC and putamen in cognitive control tasks.  We also found significant decreases between the bilateral DLPFC and insula in the Baduanjin group. DLPFC connectivity with the insula was also modulated by Baduanjin practice. The insula is one of the brain regions that play a critical role in cognitive function, including stimulustriggered reorienting of attention, self-monitoring, and emotional awareness of internal processes46. For instance, one study showed that the anterior insula contributes to the facilitation of interference resolution for emotional information in the memory process47. Investigators also believe that the anterior insula is a part of the CCN848. A recent study found that meditation practitioners exhibit decreased rsFC between the dorsal attention network, dorsal medial PFC, and the insula49. Therefore, the decrease in connectivity between the DLPFC and the insula and associated improvements in cognitive performance due to Baduanjin practice might have similar mechanisms as meditation.  It is worth noting that we did not find decreased rsFC between the DLPFC and the left putamen and insula in the Tai Chi Chuan group compared to the control group at the initial threshold (p < 0.005, cluster-corrected at FWE p < 0.05) we set. However, at a relatively less conservative threshold of voxel-wise p < 0.05 and cluster-corrected at FWE p < 0.05, we did observe decreased connectivity between the DLPFC and left putamen/insula (MNI peak coordinate (x, y, z):−15,0,21; peak Z 3.41, voxels: 4063) in Tai Chi Chuan compared to the controls. Similarly, at a less conservative threshold of voxel-wise p < 0.05 and cluster-corrected at FWE p < 0.05, we also observed decreased connectivity between the bilateral DLPFC and left ACC(MNI peak coordinate:−15,51,15; peak Z 3.49, voxels943) in the Baduanjin group compared to the control group. Given that no significant difference between the Tai Chi Chuan and Baduanjin groups was observed, we speculate that Tai Chi Chuan and Baduanjin may improve memory function through a similar neural mechanism. Future studies with larger sample sizesare needed to further test this hypothesis.

BODY.DISCUSSION.ASSOCIATION OF THE TAI CHI CHUAN MODULATION EFFECT ON CCN AND EXTENDED HIPPOCAMPUS NETWORK:
Tai Chi Chuan can significantly enhance our cognitive function by modulating the brain functioning and structure associated with cognitive processes. In recent years, the popularity of resting state functional connectivity has further endorsed the method of investigating the brain as a network. However, few studies have been applied to explore the association of Tai Chi Chuan's modulation effect on different networks within the brain.  In a previous study using the same dataset, we found Tai Chi Chuan practice can significantly increase the rsFC between the bilateral hippocampus and mPFC, and the increase is associated with improvements in memory function16. To further explore the modulation effects of Tai Chi Chuanon different networks, we performed regression analysis on hippocampus rsFC changes after Tai Chi Chuan observed in a previous study and the CCN rsFC changes observed in this study. We found no significant association, indicating that Tai Chi Chuan may modulate these two networks differently. To our best knowledge, this is the first time suggesting the different modulation effects of Tai Chi Chuan on two distinct rsFC networks, which may aid in our understanding of the complexity and variability of Tai Chi Chuan's effects on individuals.

BODY.DISCUSSION.LIMITATIONS:
There are several limitations to this study: 1) The sample size in each group is small, and 2) bothTai Chi Chuan and Baduanjin are considered mind-body exercises, making it impossible to separate the contributions of the mental and physical components. The observed connectivity patterns were consistent with the effects of both physical exercise and meditation. Future studies directly comparing the effects of exercise, meditation, and Tai Chi Chuan or Baduanjin will elucidate the specific contributions of each of the treatment components. 3) To avoid a potential cross-practice between Tai Chi Chuan and Baduanjin, subjects in one cohort were randomized to the Tai Chi Chuan or control group, and those in the other cohort were randomized to the Baduanjin or control group. We would like to emphasize that the subjects were recruited from the same community and randomized to Tai Chi Chuan, Baduanjin or control groups. We found no significant differences between groups in their baseline characteristics; therefore this should not influence our conclusion. 4) Although the Stroop task and the Wisconsin Card Sorting Task are classical tasks used to measure cognitive control functioning, previous studies suggest that both working memory and attention also play important roles in cognitive control processes8. We thus believe that the mental control task applied in our study required the involvement of cognitive control, and can reflect cognitive control functioning.  In summary, we found that TaiChi Chuan practice significantly modulates the rsFC between the CCN and the superior frontal gyrus and ACC, and Baduanjin modulates the rsFC between the CCN and the putamen and insula. This suggests that mind-body exercises such as Tai Chi Chuan and Baduanjin may be effective methods for preventing cognitive decline in older adults.

BODY.MATERIALS AND METHODS:
The experimental procedures are briefly described below. Please also see the previously published study for more details on the experimental procedure16. In that study, we investigated how longitudinal Tai Chi Chuan and Baduanjin can modulate memory function and hippocampal rsFC functional connectivity. In this study, we used the bilateral DLPFC asseeds to investigate whether the rsFC of the CCN and mental control function can be modulated by 12-week Tai Chi Chuan or Baduanjin practice, and also to determine the nature of the association between the CCN rsFC changes and hippocampal rsFC changes after Tai Chi Chuan practice. The results in this article have not been previously published.  This study was approved by the Medical Ethics Committee of Affiliated Rehabilitation Hospital, Fujian University of Traditional Chinese Medicine. The experiment was performed in accordance with approved guidelines. This study was registered in the Chinese Clinical Trial Registry (ChiCTR, http://www.chictr.org, ChiCTR-IPR-15006131). All subjects signed the written informed consent before the study began.

BODY.MATERIALS AND METHODS.SUBJECTS:
Healthy volunteers aged 50–70 were recruited from onecommunity in Gulou District, Fuzhou City, China. Two cohorts of older adults were recruited independently from the same community to avoid a potential cross-practice between Tai Chi Chuan and Baduanjin. After screening, subjects in one cohort were randomized to the Tai Chi Chuan or control group, and those in the other cohort were randomized to the Baduanjin or control group. The two cohorts started and ended at the same time. The total observation period for subjects in this study was 12 weeks.  Inclusion criteria specified that participants 1) were between 50 to 70 years old, 2) had no regular physical exercise for at least 1 year (3 months with a frequency of 3 to 4 times per week and 30 minutes per session were considered as the minimal standard for regular physical exercise) 3) were right-handed, and 4) were able to provide written informed consent. Exclusion criteria were 1) any history of stroke, severe cerebrovascular disease, musculoskeletal system disease, or sports injury related contraindications; 2) a score of <24 on the cognitive screening by the Mini-Mental State Exam (MMSE); and 3) ascore of ≥14 on the Beck Depression Inventory (BDI).

BODY.MATERIALS AND METHODS.INTERVENTION.TAI CHI CHUAN EXERCISE GROUP:
Participants in the Tai Chi Chuan group attended a 60-minute Tai Chi Chuan practice session 5 days per week for 12 weeks. The sessions consisted of 10 minutes of warm-up and review of Tai Chi principles, 30 minutes of Tai Chi Chuan exercises, 10 minutes of breathing techniques, and 10 minutes of relaxation. It was based on Yang-style 24-form.

BODY.MATERIALS AND METHODS.INTERVENTION.BADUANJIN EXERCISE GROUP:
The Baduanjin exercise took place 5 days per week for 12 weeks with each session lasting 60 minutes. The whole set of Baduanjin contained ten postures, including the starting and ending postures. Each session consisted of a warm-up followed by a review of principles, movements, breathing techniques, and relaxation. The training scheme originated from Health Qigong - Baduanjin, published by the General Administration of Sport in China50. The time schedule of the Baduanjin group was the same as that of the Tai Chi Chuan group.  Two qualified coaches with over 5 years of physical education experience taught the participants the correct Tai Chi Chuan and Baduanjin postures during the whole intervention period. In addition, two staff members supervised the training procedure to ensure the quality of the research.

BODY.MATERIALS AND METHODS.INTERVENTION.CONTROL GROUP:
A health education course was taught to the control group at the beginning of our experiment. They were asked to maintain their original physical activity habits for the 12 weeks of the experiment. They had the opportunity to receive free Tai Chi Chuan or Baduanjin training after the experiment.

BODY.MATERIALS AND METHODS.INTERVENTION.BEHAVIORAL OUTCOME MEASUREMENT:
In this study, we used mental control, a subtest from the Wechsler Memory Scale, as the behavioral outcome, which can better reflect the function of the CCN than the general score of Wechsler Memory Scale, to study the association between CCN rsFC and the behavioral outcome. Previous studies have used the mental control subtest to assess the level of impairment demonstrated by patients with dementia51. The Wechsler Memory Scale–Chinese Revision (WMS-CR)52 is designed to evaluate memory functions and is comprised of ten subtests (information, orientation, mental control, picture, recognition, visual reproduction, associative learning, touch, comprehension memory, digit span). The mental control subtest includes counting from 1to100, counting backward from 100 to 1, and adding serial 3 which is ended at 49. The participants were required to accomplish these tasks as fast as possible. Two blinded and licensed WMS-CR raters performed the measurements at the beginning and end of the study. The same materials were used in WMS-CR measurements throughout the study.

BODY.MATERIALS AND METHODS.INTERVENTION.MRI DATA ACQUISITION:
MRI scans were conducted on each subject before and after 12 weeks of treatment. The fMRI brain imaging acquisition was conducted on a 3.0 Tesla GE scanner (General Electric, Milwaukee, WI, USA) with an 8-channel phased-array head coilin the Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine (Fuzhou, China). Magnetization-prepared rapid gradient echo (MPRAGE) T1-weighted images were acquired for registration purposes with the following parameters:164 contiguous coronal slices covering the whole brain, slice thickness = 1 mm; field-of-view (FOV) = 240 mm; flip angle = 15 degree. For the BOLD resting-state fMRI, data was acquired using a T2 weighted GE-EPI sequence with the following parameters: TR = 2100 ms; TE = 30 ms; acquisition matrix = 64 × 64, FOV = 200 × 200 mm; flip angle 90 degrees; slice thickness = 3 mm, gap = 0.6 mm; voxel size = 3.125 × 3.125 × 3.6 mm3; axial slices = 42 and phases per location = 160. 5 min 36 s resting state fMRI scans were applied. During RS fMRI data acquisition, the subjects were required to keep their eyes closed and were asked to stay awake.

BODY.MATERIALS AND METHODS.STATISTICAL ANALYSIS.CLINICAL DATA ANALYSIS:
Statistical analysis was performed using SPSS 19.0 Software (SPSS Inc., Chicago, IL, USA). A threshold of p < 0.05 (2-tailed) was applied. One-way ANOVA and Chi square tests were used to analyze the baseline demographic characteristics between groups. In order to increase statistical power for the analysis, all control subjects from the two cohorts were combined into one group. In order to estimate the effects of Tai Chi Chuan and Baduanjin, we compared pre- and post-treatment mental control differences by using a mixed-model regression with subjects as a random effect and group (Tai Chi Chuan, Baduanjin, and control), time point (Week 0 and Week 12), age, gender, and years of education as fixed effects.

BODY.MATERIALS AND METHODS.STATISTICAL ANALYSIS.SEED BASED FUNCTIONAL CONNECTIVITY ANALYSIS:
All pre-processing steps and the rsFC analyses were performed using Data Processing Assistant for Resting-State fMRI (DPARSF) software (available at: http://rfmri.org/DPARSF)53 in MATLAB (Mathworks, Inc, Natick, Massachusetts). DPARSF software is based on Statistical Parametric Mapping (SPM8) (http://www.fil.ion.ucl.ac.uk/spm) and the Resting-State fMRI Data Analysis Toolkit (http://www.restfmri.net)54.  For each subject, the first 10 volumes were not analyzed to allow for signal equilibration. The remaining 150 time points from each subject were corrected for the differences in slice acquisition times. Images were then realigned, head-motion corrected, co-registered to the respective structural images for each subject, and segmented. The 6 rigid body motion parameters, white matter, and CSF signals were regressed out; images were normalized using structural image unified segmentation; and then the images were re-sampled to 3-mm cubic voxels. Subjects were excluded due to head movements exceeding 3 mm on any axis or head rotation greater than 3 degrees. After smoothing with a 6 mm full-width at half maximum (FWHM) Gaussian kernel, the linear and quadric trends of the time courses were removed. The data were bandpass filtered from 0.01 to 0.08 Hz to reduce the effects of very low frequency and high frequency physiological noise. A head motion scrubbing regressor was also used. We removed frames with FD > 0.5 mm ('scrubbing')55565758 deleting one time point before and two time points after the 'bad' time points.  Functional connectivity analysis for individual subjects was carried out in DPARSF by applying a seed-region approachusing the bilateral DLPFC (x = ±36, y = 27, z = 29, with 3 mm radius)596061. This seed was chosen based on previous research859, which demonstrated that the DLPFC can elucidate the CCN network. The averaged time course was obtained from the seed and the correlation analysis was performed in a voxel-wise way to generate the FC map. The correlation coefficient map was converted into a Fisher-Z map by Fisher's r-to-z transformation to improve the normality53.  To investigate the functional connectivity of the CCN at a group level, individual Fisher-Z functional connectivity maps obtained from the functional connectivity analysis in DPARSF were used for group analysis using SPM8 software. A full factorial design including group (Tai Chi Chuan, Baduanjin, and control group) and time (pre- and post-treatment) was applied.  To explore the association between the clinical outcomes and rsFC, we also performed regression analyses using the CCN in all subjects and mental control scores. Age, gender and years of education were included in the analysis as covariates of non-interest. A threshold of voxel-wise p < 0.005 and cluster level p < 0.05 FWE-corrected was used for all rsFC analyses.

BODY.ADDITIONAL INFORMATION:
How to cite this article: Tao, J. et al. Tai Chi Chuan and Baduanjin practice modulates functional connectivity of the cognitive control network in older adults. Sci. Rep.
7, 41581; doi: 10.1038/srep41581 (2017).  Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Question: Compared to Control group what was the result of Tai Chi Chuan group on After 12-week interventions, the mental control subscores?
Evidence: After 12-week interventions, the mental control subscores were 34.52 ± 3.17, 36.00 ± 3.78, 28.46 ± 5.76 (mean ± SD) for Tai Chi Chuan, Baduanjin and Control respectively. Mixed-model regression analysis showed significant mental control score increases in the Tai Chi Chuan and Baduanjin groups compared with the control group (Baduanjin: p < 0.001, Tai Chi Chuan: p = 0.017).
Answer: significantly increased