TITLE: 
Antiaging efficacy of melatonin-based day and night creams: a randomized, split-face, assessor-blinded proof-of-concept trial



ABSTRACT.BACKGROUND:
Skin is a complete and independent melatoninergic system. At the skin level, melatonin (Mel) acts as a relevant antioxidant and cytoprotective substance. Topical application of Mel is considered meaningful, since it can easily penetrate the stratum corneum. Exogenous Mel can be expected to represent a potent antioxidative defense system against skin aging mechanisms. Day and night creams containing Mel, carried in lipospheres (Melatosphere™), have been developed (Nutriage SPF 30 day cream and Nutriage night cream).

ABSTRACT.STUDY AIM:
The aim of this study was to evaluate the efficacy of a Mel-based cream as antiaging treatment.

ABSTRACT.SUBJECTS AND METHODS:
In a randomized, split-face, assessor-blinded, prospective 3-month study, 22 women (mean age 55 years) with moderate–severe skin aging were enrolled (clinical trial registration number: NCT03276897). Study products were applied in the morning (Nutriage day cream) and evening (Nutriage night cream) on the right or left side of the face. Primary outcomes were: 1) clinical evaluation of wrinkles’ grade (crow’s feet and nasolabial folds), surface microrelief, skin tonicity (resistance to pinching and traction, recovery after pinching) and skin dryness and 2) instrumental evaluation of skin roughness and 3D photographic documentation (Vectra H1 images system). Assessments of both clinical and instrumental evaluations were performed at baseline and after 1, 2 and 3 months of treatment by an investigator unaware of treatment allocation.

ABSTRACT.RESULTS:
All the subjects completed the study. Crow’s feet was reduced significantly (p=0.05) by −15% with the creams in comparison with the non-treated side after 3 months. At the end of the study, surface microrelief (−26.5%), skin profilometry (−13%), skin tonicity (+30%) and skin dryness (−59.5%) significantly improved with active treatment. Both products were well tolerated.

ABSTRACT.CONCLUSION:
In women with skin aging, Mel-based creams improved significantly skin tonicity and skin hydration with a significant reduction in skin roughness, supporting the skin antiaging effect of this molecule applied topically.

BODY.INTRODUCTION:
Skin is a complete and independent melatoninergic system.1,2 Skin can synthesize melatonin (Mel), and skin cells express melatonin receptors MT1 and MT2.3,4 Mel is a pleiotropic molecule.5 At the skin level, Mel acts as a relevant antioxidant and cytoprotective substance and plays a fundamental role in maintaining the homeostasis of the skin.6 Skin Mel, and most of its metabolites, exerts a strong protection against oxidative stress and ultraviolet radiation.2,7–9 Cutaneous melatoninergic and metabolic systems can act as auto/paracrine protectors against environmentally induced damage.10 Skin rapidly metabolizes Mel.11 Therefore, exogenous Mel can be expected to represent one of the most potent antioxidative defense systems against skin aging mechanisms.12 Oral administration of Mel shows a prominent first-pass effect with very low systemic level.13,14 Topical administration could circumvent this problem. Topical application of Mel is considered meaningful, since it can penetrate the stratum corneum due to its distinct lipophilic chemical structure.15 So far, no clinical data regarding the efficacy of topical Mel as an antiaging molecule has been reported. Recently, day and night creams containing Mel, vehiculated in lipospheres (Melatosphere™), have been developed (Nutriage day cream and Nutriage night cream; Cantabria Labs, Difa Cooper, Caronno Pertusella, VA, Italy). The liposphere carrier system could further improve the penetration of the Mel molecules through the skin layers.16

BODY.STUDY AIM:
The aim of this study was to assess the antiaging effect of 0.1% Mel-based creams (a day formulation and a night formulation).

BODY.SUBJECTS AND METHODS:
We planned a randomized, split-face, assessor-blinded, prospective 3-month study.
BODY.SUBJECTS:
In all, 22 women (mean age 55 years) with moderate–severe skin aging were enrolled after receiving their written informed consent. The subjects also provided consent to the publication of any pictures. The inclusion criterion was women aged ≥45 years with moderate–severe facial skin aging (Glogau score of 3 or 4). The exclusion criteria were any acute or chronic skin conditions, which could interfere with the parameters of evaluation and a positive history of allergic contact dermatitis to any of the component of the creams. Study products were applied one in the morning (Nutriage day cream) and one in the evening (Nutriage night cream) on the right or left side of the face according to a randomization list. The randomization list was generated using a dedicated software. The local institutional review board (IRB; Independent Ethical Committee, c/o Derming Clinic, Milan, Italy) approved the trial protocol on March 10, 2017 (clinical trial registration Number: NCT03276897). The study was conducted in accordance with the ethical principles of the Declaration of Helsinki and consistent with the Good Clinical Practice (GCP) regulatory requirements.17 Compliance to the treatment was evaluated counting the returned used cream blisters at visits during months 1, 2 and 3.

BODY.STUDY OUTCOMES:
Primary outcomes were: 1) clinical evaluation of wrinkles’ grade (crow’s feet and nasolabial folds), surface microrelief, skin firmness/tonicity (resistance to pinching and traction, recovery after pinching) and skin dryness and 2) instrumental evaluation of skin roughness and 3D photographic documentation (Vectra H1 images system). Assessments of both clinical and instrumental evaluations were performed at baseline and after 1, 2 and 3 months of treatment by an investigator unaware of treatment allocation. Secondary outcome was the local tolerability of the products evaluated. Sky dryness was evaluated using a 6-point score scale (from 0: very hydrated to 5: very dry). Resistance to traction, resistance to pinching and recovery after pinching were evaluated using a 5-point score scale (from 0: very strong to 4: very weak). Wrinkles’ grade and surface microrelief were scored visually on the base of high-definition pictures using the Glogau18 (crow’s feet) and Monheit et al19 (nasolabial) photographic 6-point scales. Surface microrelief was evaluated using a 4-point scale (from 1: very regular to 4: very irregular). Instrumental evaluation of wrinkles using profilometry was performed at the crow’s feet area using Primos compact portable device (GFMesstechinck, Konstanz; Germany). The Primos software is able to elaborate 3D representations of skin wrinkles and to measure skin principal profilometric parameters. Pictures with the Vectra device were performed in a standardized (distance and illumination) manner with the subjects with open eyes and relaxed face muscles. Finally, the Spiderming™ radial graph was used to “visualize” the global antiaging effect of the treatment.20 This graph (Derming S.r.l. Clinical Research Institute, Milan, Italy) allows the results to be evaluated and the effect of an antiaging cosmetic product to be visualized, taking into account that a smaller area of the graph coincides with a younger skin; the activity of the products in terms of antiaging activity could be quantified.

BODY.STATISTICAL ANALYSIS AND SAMPLE SIZE CALCULATION:
Statistical analysis was performed using GraphPad Statistical Software (GraphPad Software, Inc., La Jolla, CA, USA). Continuous variables were expressed as mean ± standard deviation (SD). The primary outcomes of the study were to evaluate scores of skin parameters at month 3 in comparison with baseline values (intragroup evaluation) using paired nonparametric test (Wilcoxon test) and to compare at month 3, the same skin parameters between treated and non-treated sides (intergroup evaluation) using unpaired nonparametric test (Mann–Whitney test). The secondary outcome was to evaluate the evolution of the skin variables during the study at each visit (baseline, month 1, month 2 and month 3) using analysis of variance (ANOVA) test for repeated measures. Inferential statistical analysis was done based on intention-to-treat principle. In view of the proof-of-concept nature of the present trial, a formal sample size calculation was not performed. We decided to enroll at least 20 evaluable subjects.


BODY.RESULTS:
The trial was conducted between March 2017 and September 2017 in an outpatient derma-esthetic clinic. All the subjects completed the study. In comparison with baseline value, skin dryness was significantly (p=0.001; Wilcoxon test) reduced by 59.5% (from 3.6±0.7 to 1.5±0.4) on the side treated with Mel creams. Skin dryness score was significantly lower in the active-treated side in comparison with the non-treated side (p=0.01; Mann–Whitney U test; 1.5±0.4 vs 3.5±0.6; Figure 1A). Skin tonicity (resistance to traction, resistance to pinching and recovery after pinching) significantly improved by 33% in comparison with the baseline value on the side treated with Mel creams only (from 3.0±0.5 to 2.1±0.4; Figure 1B and C). Crow’s feet photographic severity score was reduced significantly (p=0.05) by −15% with the creams in comparison with both baseline value (2.3±0.2 vs. 2.7±0.3) and value of the non-treated side after 3 months. Surface microrelief photographic score was significantly reduced by −26.5% at the end of treatment in comparison with both baseline value (2.4±0.5 vs. 3.4±0.6; p=0.001; Wilcoxon test) and value of the control side at month 3 (2.4±0.5 vs. 3.3±0.6; p=0.001; Mann–Whitney U test). Significant improvements were also observed starting from month 1 on the sides treated with the active product (ANOVA test; data not shown). For all the skin parameters evaluated, nonstatistical significant modifications have been observed in the control side comparing baseline and 3-month values. Figure 2 shows the evolution of the Spiderming graph with a significant reduction in the total area after active treatment in comparison with baseline and non-treated sides. Skin profilometry evaluation showed a −13% reduction in roughness profile, −13% reduction in wrinkles’ maximum depth and −7.2% reduction in total wrinkles’ height in comparison with baseline and control sides. However, these reductions, although clinically relevant, did not reach a statistical significant difference. Figure 3 shows two subjects’ pictures performed with Vectra at baseline and after 3 months of treatment. Both products (day and night creams) were well tolerated. No side effects were reported. Compliance to the treatment was >90% in all the enrolled subjects.

BODY.DISCUSSION:
Mel is considered as a strong antioxidant molecule, and it is one of the most potent ·OH radical scavenger in nature.21 Experimental studies have shown that Mel is a stronger radical scavenger than vitamins C and E.22 Extrapineal Mel production has been demonstrated in bone marrow, cerebrospinal fluid, ovary, eye, lymphocytes, gastric mucosa and skin.23–25 Human skin and skin-derived cells can produce and metabolize Mel, and Mel exerts many effects on cell growth regulation and skin tissue homeostasis via Mel receptors MT1 and MT2.26 Skin can produce large amount of Mel.27,28 The protective effects of Mel against ultraviolet (UV) solar skin damage are mediated through direct and indirect radical scavenging and antioxidative enzyme-stimulating actions.29 Several data support the concept that at the skin level. Mel and its metabolites serve as protectors against physicochemical (oxidative damage, ultraviolet radiation [UVR], chemicals) and biological stressors. The antioxidant action of Mel has also genomic influences, regulating the expression of several genes.30 Mel stimulates both antioxidant enzyme activity and cellular mRNA levels for these enzymes.31 Mel could also protect DNA from oxidative damage.32 Mel can maintain mitochondrial membrane potential in UVR-exposed keratinocytes.33,34 Therefore, Mel is a good cytoprotective and antiaging molecule.35 As stated by Fischer et al,36 for application in clinical dermatology, exogenous Mel should be used topically rather than orally, since orally administered Mel appears in rather low levels in the blood due to prominent first-pass degradation in the liver, thus limiting skin access. Topical administration circumvents this problem. Therefore, in contrast with oral administration, topical application of Mel is considered meaningful, since it can penetrate the stratum corneum due to its distinct lipophilic chemical structure. In the present study, the tested products contain Mel molecules incorporated in lipospheres (Melatosphere) with the aim to further increase the skin penetration of the molecule. So far, no clinical data regarding the efficacy of topical Mel as an antiaging molecule has been reported. In our study, we evaluated for the first time, the efficacy of two Mel-based products in women with skin aging. The results we observed show that in comparison with the non-treated control side, these topical Mel formulations improve skin hydration and skin tonicity with a clinical improvement in the aspect of wrinkles. Some limitations should be taken into account in evaluating our results. First, this is a proof-of-concept study. Future trials with larger sample size are warranted to evaluate the real clinical effects of these products in the treatment of skin aging. A second limitation of this study is that this is not a double-blind trial. However, to increase the internal validity of our results, we adopted a split face, assessor-blinded study design.

BODY.CONCLUSION:
In women with skin aging, Mel-based creams improved significantly skin tonicity and skin hydration with a significant reduction in skin roughness, supporting the skin antiaging effect of this molecule applied topically.

Question: Compared to Not treated another half of the face what was the result of Day and night creams containing Mel, carried in lipospheres (Melatosphere™) on one half of the face on Surface microrelief photographic score?
Evidence: Skin dryness score was significantly lower in the active-treated side in comparison with the non-treated side (p=0.01; Mann–Whitney U test; 1.5±0.4 vs 3.5±0.6; Figure 1A).
Answer: significantly decreased