Question: STED microscopy was developed by a man who is one of the directors of what? 
Context: Live cell imaging is the study of living cells using time-lapse microscopy.  It is used by scientists to obtain a better understanding of biological function through the study of cellular dynamics.  Live cell imaging was pioneered in first decade of the 20th century.  One of the first time-lapse microcinematographic films of cells ever made was made by Julius Ries, showing the fertilization and development of the sea urchin egg.  Since then, several microscopy methods have been developed which allow researchers to study living cells in greater detail with less effort.  A newer type of imaging utilizing quantum dots have been used as they are shown to be more stable. Entamoeba coli is a non-pathogenic species of "Entamoeba" that frequently exists as a commensal parasite in the human gastrointestinal tract.  "E. coli" (not to be confused with the bacterium "Escherichia coli") is important in medicine because it can be confused during microscopic examination of stained stool specimens with the pathogenic "Entamoeba histolytica".  This amoeba does not move much by the use of its pseudopod, and creates a ""sur place" (non-progressive) movement" inside the large intestine.  Usually, the amoeba is immobile, and keeps its round shape.  This amoeba, in its trophozoite stage, is only visible in fresh, unfixed stool specimens.  Sometimes the "Entamoeba coli" have parasites as well.  One is the fungus "Sphaerita" spp.  This fungus lives in the cytoplasm of the "E. coli".  While this differentiation is typically done by visual examination of the parasitic cysts via light microscopy, new methods using molecular biology techniques have been developed. Stefan Walter Hell (born 23 December 1962) is a Romanian-born German physicist and one of the directors of the Max Planck Institute for Biophysical Chemistry in Göttingen, Germany.  He received the Nobel Prize in Chemistry in 2014 "for the development of super-resolved fluorescence microscopy", together with Eric Betzig and William Moerner. The Center for Probing the Nanoscale (CPN) at Stanford University was founded in 2004 by researchers from Stanford University and IBM.  The center is one of the National Science Foundation (NSF) Nanoscale Science and Engineering Centers (NSEC).  The goal of the center is to develop and apply novel nanoprobes that dramatically improve our capability to observe, manipulate, and control nanoscale objects and phenomena.  Developed technology will be transferred to industry for commercial implementation.  Nanoprobe development and applications are under way in five theme groups, focusing on Individual Nanomagnet Characterization, Nanoscale Magnetic Resonance Imaging, Nanoscale Electrical Imaging, Plasmonic Scanning Tunneling Microscopy and BioProbes. Stimulated emission depletion (STED) microscopy is one of the techniques that make up super-resolution microscopy.  It creates super-resolution images by the selective deactivation of fluorophores, minimising the area of illumination at the focal point, and thus enhancing the achievable resolution for a given system.  It was developed by Stefan W. Hell and Jan Wichmann in 1994, and was first experimentally demonstrated by Hell and Thomas Klar in 1999.  Hell was awarded the Nobel Prize in Chemistry in 2014 for its development.  In 1986, V.A. Okhonin (Institute of Biophysics, USSR Academy of Sciences, Siberian Branch, Krasnoyarsk) had patented the STED idea.  This patent was, perhaps, unknown to Hell and Wichmann in 1994. Mega Man 9, known in Japan as Rockman 9: Yabō no Fukkatsu!!  (ロックマン9 野望の復活!!  , Rokkuman Nain Yabō no Fukkatsu!!  , lit.  "Rockman 9: Revival of Ambition!!")  , is an action-platform video game developed by Capcom and Inti Creates.  It is the ninth numbered game in the original "Mega Man" series.  "Mega Man 9" is the first, new home console game in the original "Mega Man" series since "Mega Man 8" and "Mega Man & Bass", which were released at least one decade earlier.  "Mega Man 9" was the first game in the series not to have a physical release, and was initially released only on the downloadable gaming services WiiWare, PlayStation Network (PSN), and Xbox Live Arcade (XBLA).  In June 2017, it was announced that Mega Man 9 and 10 would have a physical release with their inclusion in Mega Man Legacy Collection 2 for PlayStation 4, Xbox One and PC. Campbell was born and raised in Detroit, Michigan.  His father, J. V. Campbell, was a member of the Supreme Court of the state of Michigan and a law professor at the University of Michigan.  Douglas Campbell graduated from Detroit High School in 1878, going on to study at the University of Michigan.  He studied botany, learning new microscopy techniques, and becoming interested in cryptogramic (deciduous) ferns.  He received his master's degree in 1882, and taught botany at Detroit High School while he completed his PhD research.  He received his PhD in 1886, then travelled to Germany to learn more microscopy techniques.  He developed a technique to embed plant material in paraffin to make fine cross-sections; he was one of the first if not the first to study plant specimens using this technique, which had been newly developed by zoologists.  He was also a pioneer in the study of microscopic specimens using vital stains. Low-energy electron microscopy, or LEEM, is an analytical surface science technique invented by Ernst Bauer in 1962, however, not fully developed (by Ernst Bauer and Wolfgang Telieps) until 1985.  LEEM is a technique used by surface scientists to image atomically clean surfaces, atom-surface interactions, and thin (crystalline) films.  In LEEM, high-energy electrons (15-20 keV) are emitted from an electron gun, focused using a set of condenser optics, and sent through a magnetic beam deflector (usually 60˚ or 90˚).  The “fast” electrons travel through an objective lens and begin decelerating to low energies (1-100 eV) near the sample surface because the sample is held at a potential near that of the gun.  The low-energy electrons are now termed “surface-sensitive” and the near-surface sampling depth can be varied by tuning the energy of the incident electrons (difference between the sample and gun potentials minus the work functions of the sample and system).  The low-energy elastically backscattered electrons travel back through the objective lens, reaccelerate to the gun voltage (because the objective lens is grounded), and pass through the beam separator again.  However, now the electrons travel away from the condenser optics and into the projector lenses.  Imaging of the back focal plane of the objective lens into the object plane of the projector lens (using an intermediate lens) produces a diffraction pattern (low-energy electron diffraction, LEED) at the imaging plane and recorded in a number of different ways.  The intensity distribution of the diffraction pattern will depend on the periodicity at the sample surface and is a direct result of the wave nature of the electrons.  One can produce individual images of the diffraction pattern spot intensities by turning off the intermediate lens and inserting a contrast aperture in the back focal plane of the objective lens (or, in state-of-the-art instruments, in the center of the separator, as chosen by the excitation of the objective lens), thus allowing for real-time observations of dynamic processes at surfaces.  Such phenomena include (but are not limited to): tomography, phase transitions, adsorption, reaction, segregation, thin film growth, etching, strain relief, sublimation, and magnetic microstructure.  These investigations are only possible because of the accessibility of the sample; allowing for a wide variety of in situ studies over a wide temperature range. Antonie Philips van Leeuwenhoek ( , ] ; 24 October 1632 – 26 August 1723) was a Dutch businessman, scientist, and one of the notable representatives of the Golden Age of Dutch science and technology.  A largely self-taught man in science, he is commonly known as "the Father of Microbiology", and often considered to be the first acknowledged microscopist and microbiologist.  Van Leeuwenhoek is best known for his pioneering work in the field of microscopy and for his contributions toward the establishment of microbiology as a scientific discipline. Alcian blue or alcian blue ( ) is any member of a family of polyvalent basic dyes, of which the Alcian blue 8G (also called Ingrain blue 1, and C.I. 74240, formerly called Alcian blue 8GX from the name of a batch of an ICI product) has been historically the most common and the most reliable member.  It is used to stain acidic polysaccharides such as glycosaminoglycans in cartilages and other body structures, some types of mucopolysaccharides, sialylated glycocalyx of cells etc.  For many of these targets it is one of the most widely used cationic dyes for both light and electron microscopy.  Use of alcian blue has historically been a popular staining method in histology especially for light microscopy in paraffin embedded sections and in semithin resin sections.  The tissue parts that specifically stain by this dye become blue to bluish-green after staining and are called "Alcianophilic" (akin to "eosinophilic" or "sudanophilic").  Alcian blue staining can be combined with H&E staining, PAS staining and van Gieson staining methods.  Alcian blue can be used to quantitate acidic glycans both in microspectrophotometric quantitation in solution or for staining glycoproteins in polyacrylamide gels or on western blots. Biochemists had used it to assay acid polysaccharides in urine since the 1960s for diagnosis of diseases like mucopolysaccharidosis but from 1970's, partly due to lack of availability of Alcian and partly due to length and tediousness of the procedure, alternative methods had to be developed e.g. Dimethyl methylene blue (DMB) method.
Answer: Max Planck Institute