anthonyyazdaniml/gliner-biomed-curated-corpus huggingface dataset:
PURPOSE:To enable production of a phenylglycoside with a high production efficiency by transforming a gentianaceous plant cell with an Ri plasmid held by a bacterium of the genus Agrobacterium and then culturing the resultant hairy roots. CONSTITUTION:Apical buds or stem nodal parts are cut out of a gentianaceous plant body belonging to the genus Swertia, etc., and cultured. An Ri plasmid fragment held by a bacterium belonging to the genus Agrobacterium is then inoculated into a gentianaceous plant gene and cultured to develop hairy roots. Hairy roots having a high proliferation rate and many branches are selected from the resultant hairy roots, further cultured in a culture medium and proliferated in a large amount. The obtained hairy roots after completing the culture are extracted with an organic solvent such as ethanol or water. After distilling away the solvent, the residue is treated by column chromatography to recover the objective phenylglycoside. Thereby, since efficient production can be carried out, the aforementioned method is extremely suitable as an industrial method for producing the phenylglycoside.
Blood will be drawn to assess the seropersistence of TBE virus antibodies at 82, 94, 106 and 118 months after the first booster vaccination with FSME-IMMUN 0.5ml administered during the first precursor study. Timing of the second booster vaccination will depend on the level of serum TBE antibodies observed during the study. Blood will be drawn 21 - 35 days after vaccination to assess the booster response.
In breast conservative surgery, it is sometimes difficult to decide whether the cauterised tissue at the inked margin represents normal / hyperplastic or neoplastic tissue. We retrospectively assessed the value of ER, PR, CK5 and CK14 IHC in clarifying the nature of cauterised tissues at the margins concerning 34 lesions of 23 patients. 27 cases belonged to lesions that could not be adequately classified on the basis of the HE stains. Two thirds of them could be classified as non-neoplastic or neoplastic and two thirds of the remaining could be favourised as neoplastic or non-neoplastic, with 3/27 cases remaining uncertain. All 4 IHC reactions were helpful in classifying the lesions in almost half of the cases. However, 3 or 4 immunostains were supportive of the classification in 19/27. The most useful stains were the keratins, generally demonstrating a matching pattern of cell labelling with CK5 and CK14. ER and PR were somewhat less useful in classifying uncertain lesions. Considering all the 27 questionable lesions, IHC with ER, PR, CK5 and CK14 clarified the lesions at the cauterised margins in 23 cases. Taken all these considerations into account, CK5, CK14, PR and ER IHC may help in distinguishing between cautery damaged neoplastic and non-neoplastic tissues. All four IHC may yield the best support for decision making, but CK5 and/or CK14 may be sufficient in their own. The essential approach is that the results must be interpreted with caution, in the context of the given patient's disease, to avoid misinterpretations.
Study design type: Multi-center, observational, case-control study based on propensity score matching. The leading unit is Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, and the participating unit is The First Hospital of Harbin Medical University.

Record general clinical information, surgical-anesthesia-related information, recovery indicators, pain status, adverse events in PACU, and occurrences of postoperative delirium of all study subjects meeting the inclusion criteria of this study. Based on the occurrence of postoperative delirium, the subjects will be divided into delirium group and non-delirium group for univariate analysis. Univariate factors with statistical significance will be included in a multiple logistic regression analysis to further identify risk factors for postoperative delirium. Some selected factors can undergo ROC curve analysis to calculate the optimal cutoff value. Due to the limited number of subjects experiencing postoperative delirium, propensity score matching will be used to improve the statistical efficiency for analysis of risk factors for postoperative delirium. Furthermore, for patients with postoperative delirium, a small amount of blood samples will be collected by the researchers for analysis of inflammatory factors and other components.
A injection dosage form containing pure peanut oil in controlling disorders that arise in cells, or correct errors in the genetic code that cause mental, and physical disorders also in the process Mitosis cells divide, or forms identical cells, restore cells hereditary material that's scattered throughout the nucleus, restore aging, DNA restore torn tissue, muscle, alter the code and erase hundreds of inherited mental, and physical aids, deadly diseases, by enlarge cells, asthma, heart problems, child bearing, enlarge nose, lip, breast, penis, ear lobe, hearing, lungs, veins, gallstones, alter mature nerve cells, reproduce, or replace damage, or destroyed cells, replace worn out, or disease tissue, muscles, heart, cataracts, a stump of an amputated limb to re-grow. This will change the hereditary process of humans with diseases. These disorders are provided in an acceptable form. The dosage form comprises pure peanut oil which is applies in an amount that provides for a relatively period during which the composition is being excepted by a patient, but which allows substantially immediate release of the pure peanut oil after the composition has been injected. This will correct diseases that result from foreign invader organisms call pathogens, also that threaten our homeostasis-identified molecules or outer surfaces the membrane.  
     The development of DNA technology and a good function gene can be inserted into the cells of a patient, and can correct genetic defects, also give new function to the cell. This is the way type of viruses attack humans cells. The adjacent tissue this cell is the basic building block fundamental unit of the body chromosomes of cell membranes and the nucleus, cytoplasm within the cell membrane and the nucleus holds the genetic material chromosomes this will cure all defects, disorders, diseases of all kind, like cataracts and cancer.
Parents will receive STS, which will include 1) Individualized virtual communication training and a booster session delivered by a traffic safety communication specialist; and 2) An online parent-teen safe driving communication guide. In addition, the Azūga™ in-vehicle device and app will be installed as described above and all feedback features will be enabled. Three types of feedback will be provided to teens: 1) Direct audio feedback; 2) Detailed cumulative driving data; and 3) A customized weekly driving summary report. Parents in this group will receive access to the teen's cumulative driving data and a weekly driving summary report. Additionally, a wireless mini-camera will be installed on the dashboard in teen's car to identify the participating driver using facial verification technology.
Tumour evolution with acquisition of more aggressive disease characteristics is a hallmark of disseminated cancer. Metastatic pancreatic neuroendocrine tumours (PanNETs) in particular may progress from a low/intermediate to a high-grade disease. The aim of this work was to understand the molecular mechanisms underlying metastatic progression as well as PanNET transformation from a low/intermediate to a high-grade disease. We performed multi-omics analysis (genome/exome sequencing, total RNA-sequencing and methylation array) of 32 longitudinal samples from six patients with metastatic low/intermediate grade PanNET. The clonal composition of tumour lesions and underlying phylogeny of each patient were determined with bioinformatics analyses. Findings were validated in post-alkylating chemotherapy samples from 24 patients with PanNET using targeted next generation sequencing. We validate the current PanNET evolutionary model with MEN1 inactivation that occurs very early in tumourigenesis. This was followed by pronounced genetic diversity on both spatial and temporal levels, with parallel and convergent tumour evolution involving the ATRX/DAXX and mechanistic target of the rapamycin (mTOR) pathways. Following alkylating chemotherapy treatment, some PanNETs developed mismatch repair deficiency and acquired a hypermutational phenotype. This was validated among 16 patients with PanNET who had high-grade progression after alkylating chemotherapy, of whom eight had a tumour mutational burden >50 (50%). In comparison, among the eight patients who did not show high-grade progression, 0 had a tumour mutational burden >50 (0%; odds ratio 'infinite', 95% confidence interval 1.8 to 'infinite', p = 0.02). Our findings contribute to broaden the understanding of metastatic/high-grade PanNETs and suggests that therapy driven disease evolution is an important hallmark of this disease. © 2024 The Author(s). The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.
Abstract A system and method for use in iontophoretic anesthesia of a tympanic membrane are disclosed. The system generally includes an earplug and an electrode device. The earplug includes at least one sealing member for sealing the earplug in an ear canal. The sealing member includes microholes which vent fluid above a certain pressure threshold. A headset may connect the earplug to a second earplug. The method involves using the system on a human or animal subject.
Participants will receive 2 mg BOS, single dose, orally on Day 1 of Period 1 under fasted condition (Treatment A). Two days later, participants will receive 2 mg BOS single dose, orally on Day 1 of Period 2 under fed condition (Treatment B).
Cohort B will include LRRK2 G2019S carriers, idiopathic PD patients, and heathy volunteers. It will involve only a blood sample collection.
NanoRay Biotech is developing a novel investigational therapeutic system called Auger Molecular Therapy (AMT), which consists of a drug, Iododeoxyuridine (IUdR), and a medical device, AUTRON Therapy System, intended for the palliative treatment of malignant cutaneous lesions. IUdR is an iodinated analogue of deoxyuridine, which can be incorporated into DNA of cancer cells because it is a thymidine analogue. The AUTRON Therapy System is a complete, standalone low energy X-Ray radiation source (80 kVp, 100 μA) intended for local irradiation. The AUTRON Therapy System contains a novel design of transmission type X-Ray tube, which allows electron beams to pass through the metal target and generates characteristic X-Ray similar to synchrotron radiation.

The combination product is intended to induce Auger effect to augment the damage on tumor cells, while lowering the adverse effect on normal cells. IUdR is an iodinated thymidine analogue that is preferentially incorporated into DNA in rapid growing cells (such as cancer cells) after administration. The AUTRON Therapy System generates characteristic X-Ray photons around 33.4 keV, matching the K-edge energy of Iodine (33.17 keV), which can efficiently induce emissions of Auger electrons when hitting the Iodine atoms on IUdR in cancer DNA, resulting in extensive DNA damage and cancer cell death. Furthermore, the characteristic X-Ray photons generated by the AUTRON Therapy System have a low penetration rate in biological materials, which give the maximum dose on superficial lesions and much reduced dose on peripheral or deep normal tissues, fitting the need on the management of malignant cutaneous lesions.
New agents against lice and vermin contain 0.05-0.1% cupric oleate dissolved in a non-inflammable organic solvent, the soln. opt. being diluted with non-inflammable organic solvents, wetting agents or ointment bases. Lice (including nits) and vermin such as fleas are killed within 20-30 mins. of application to humans or animals. The prod. has an anti-itching effect, so that scratching does not occur and injury due to penetration of scratches by the agent is minimised. Cupric oleate is non-toxic to humans, and use of non-inflammable solvents eliminates fire disk. Pref. organic solvents are aromatic alcohols such as benzyl alcohol, esters of unsatd. dicarboxylic acids such as diethyl maleate, esters of oxycarboxylic acids such as ethyl lactate, and terpene alcohols such as menthol. Partic. pref. agents contain cupric oleate and menthol dissolved in organic, non-inflammable solvents and/or wetting agents.
Obesity rates continue to rise globally, posing a significant public health concern. Current treatments often lack long-term effectiveness, necessitating the exploration of new approaches. This study examines the effectiveness of a modified treatment method called Cognitive Behavioral Therapy modified as FIRE method (CBT-F), which combines cognitive behavioral therapy with pharmacotherapy. The study involves 62 women seeking weight loss treatment, divided into a CBT-F group and a control group receiving only pharmacotherapy. Anthropometric measures and blood chemistry data were collected over an average follow-up period of 68.5 days. The results demonstrate that the CBT-F group achieved significantly greater weight loss compared to the control group. No notable differences were observed in blood chemistry data. The combination of CBT-F and pharmacotherapy offers a comprehensive and planned approach to obesity treatment by addressing psychological factors and leveraging the effects of medication. Modules specifically designed to handle medication side effects and changes in eating behavior may contribute to treatment success and sustainability. Although this study focused on women, future research should examine the effectiveness of CBT-F in diverse populations. CBT-F shows promise as an alternative or complementary treatment option for individuals who have undergone CBT for extended periods or struggle with lifestyle changes. Overall, the findings suggest that CBT-F, with its shorter treatment duration and immediate effects of pharmacotherapy, holds potential as an effective and sustainable approach to obesity treatment. Further studies are necessary to validate these findings and expand the evidence base for this novel treatment.
A therapeutic agent said agent being an agonist to a receptor of the cytokine receptor family such as the h* small Greek beta *c or common * small Greek beta * receptor of IL-3, IL-5 and GM-CSF. Each of the members of the cytokine receptor family having an extracellular receptor module (CRM) composed of two discrete folding domains (CRD). It has been found that a first of the two CRDs, in the case of h* small Greek beta *c being * small Greek beta *4, is a switching CRD so that conformational changes in the switching CRD lead to activation. A second of the CRDs, in the case of h* small Greek beta *c being * small Greek beta *3, being an inhibiting CRD. Under normal conditions the CRM is in an inactive state unless activated by ligand. The agonist property being a result of a change in the switching CRD from its inactive state to an active state leading to a signal being produced to effect said agonist property, and may be a result of blocking the inhibitory effect of the inhibiting CRD on the switching CRD or may be a result of inducing a change to the switching CRD by itself.
Cirrhotic patients may show minimal hepatic encephalopathy (MHE), with mild cognitive impairment and motor deficits. Hyperammonemia and inflammation are the main contributors to the cognitive and motor alterations of MHE. Hyperammonemic rats reproduce these alterations. There are no specific treatments for the neurological alterations of MHE. Extracellular vesicles from mesenchymal stem cells (MSC-EVs) are promising to treat inflammatory and immune diseases. We aimed to assess whether treatment of hyperammonemic rats with MSC-EVs reduced neuroinflammation in cerebellum and restored motor coordination and to study the mechanisms involved. The effects of MSC-EVs were studied in vivo by intravenous injection to hyperammonemic rats and ex vivo in cerebellar slices. Motor coordination was analyzed using the beam walking test. Effects on neuroinflammation were assessed by immunohistochemistry, immunofluorescence and Western blot. Injection of MSC-EVs reduced microglia and astrocytes activation in cerebellum and restored motor coordination in hyperammonemic rats. Ex vivo experiments show that MSC-EVs normalize pro-inflammatory factors, including TNFα, NF-kB activation and the activation of two key pathways leading to motor incoordination (TNFR1-NF-kB-glutaminase-GAT3 and TNFR1-CCL2-BDNF-TrkB-KCC2). TGFβ in the EVs was necessary for these beneficial effects. MSC-EVs treatment reverse neuroinflammation in the cerebellum of hyperammonemic rats and the underlying mechanisms leading to motor incoordination. Therapy with MSC-EVs may be useful to improve motor function in patients with MHE.
PURPOSE:To obtain a new protein, derived from a biological tissue and cell, capable of enhancing the production of an anti-DNA antibody and binding to the DNA and useful for understanding the morbid state, diagnosing and treating autoimmune diseases caused by abnormal production, transudation, etc., of nucleic acids such as the DNA. CONSTITUTION:A KML1-7 cell derived from a lymphoid established cell established as a cell strain capable of producing and releasing a factor enhancing the production of an anti-DNA antibody from the whole cell population of a tumescent lymph node in an MRL/l mouse is cultured in the presence of blood serum until a confluent state is attained. The cultured KML1-7 cell is then collected by centrifugation, transferred to a serum-free culture medium, suspended and subsequently cultured in a CO2 incubator for 3 days. The resultant culture solution is centrifuged to collect the culture supernatant and a serine protease inhibitor is directly added thereto. The prepared mixture is then concentrated with an ultrafiltration membrane and the resultant concentrate is adsorbed on an anion exchange resin. Common salt at >=0.2M concentration is added to purify the eluted fraction by high-performance liquid chromatography to afford the objective protein capable of enhancing the production of the anti-DNA antibody and binding to the DNA.
ETHNOPHARMACOLOGICAL RELEVANCE: Borneol is a long-established traditional Chinese medicine that has been found to be effective in treating pain and itchy skin. However, whether borneol has a therapeutic effect on chronic itch and its related mechanisms remain unclear.
AIM OF THE STUDY: To investigate the antipruritic effect of borneol and its molecular mechanism.
MATERIALS AND METHODS: DrugBAN framework and molecular docking were applied to predict the targets of borneol, and the calcium imaging or patch-clamp recording analysis were used to detect the effects of borneol on TRPA1, TRPM8 or TRPV3 channels in HEK293T cells. In addition, various mouse models of acute itch and chronic itch were established to evaluate the antipruritic effects of borneol on C57BL/6J mice. Then, the borneol-induced pruritic relief was further investigated in Trpa1-/-, Trpm8-/-, or Trpa1-/-/Trpm8-/- mice. The effects of borneol on the activation of TRPM8 and the inhibition of TRPA1 were also measured in dorsal root ganglia neurons of wild-type (WT), Trpm8-/- and Trpv1-/- mice. Lastly, a randomized, double-blind study of adult patients was conducted to evaluate the clinical antipruritic effect of borneol.
RESULTS: TRPA1, TRPV3 and TRPM8 are the potential targets of borneol according to the results of DrugBAN algorithm and molecular docking. Calcium imaging and patch-clamp recording analysis demonstrated that borneol activates TRPM8 channel-induced cell excitability and inhibits TRPA1 channel-mediated cell excitability in transfected HEK293T cells. Animal behavior analysis showed that borneol can significantly reduce acute and chronic itch behavior in C57BL/6J mice, but this effect was eliminated in Trpa1-/-, Trpm8-/- mice, or at least in Trpa1-/-/Trpm8-/- mice. Borneol elicits TRPM8 channel induced [Ca2+]i responses but inhibits AITC or SADBE-induced activation of TRPA1 channels in dorsal root ganglia neurons of WT and Trpv1-/- mice, respectively. Furthermore, the clinical results indicated that borneol could reduce itching symptoms in patients and its efficacy is similar to that of menthol.
CONCLUSION: Borneol has therapeutic effects on multiple pruritus models in mice and patients with chronic itch, and the mechanism may be through inhibiting TRPA1 and activating TRPM8.
Three randomized, double-blind, placebo-controlled trials with durations from 16 to 26 weeks were conducted to evaluate the use of pioglitazone hydrochloride as monotherapy in patients with type 2 diabetes. These trials examined pioglitazone at doses up to 45 mg or placebo once daily in a total of 865 patients. 

In a 26 week dose-ranging monotherapy trial, 408 patients with type 2 diabetes were randomized to receive 7.5 mg, 15 mg, 30 mg, or 45 mg of pioglitazone, or placebo once daily. Therapy with any previous antidiabetic agent was discontinued eight weeks prior to the double-blind period. Treatment with 15 mg, 30 mg, and 45 mg of pioglitazone produced statistically significant improvements in HbA1c and fasting plasma glucose (FPG) at endpoint compared to placebo (see Figure 1, Table 17). 

Figure 1 shows the time course for changes in HbA1c in this 26 week study.

Figure 1: Mean Change from Baseline for HbA1c in a 26 Week Placebo-Controlled Dose-Ranging Study (Observed Values)

In a 24 week placebo-controlled monotherapy trial, 260 patients with type 2 diabetes were randomized to one of two forced-titration pioglitazone hydrochloride treatment groups or a mock-titration placebo group. Therapy with any previous antidiabetic agent was discontinued six weeks prior to the double-blind period. In one pioglitazone hydrochloride treatment group, patients received an initial dose of 7.5 mg once daily. After four weeks, the dose was increased to 15 mg once daily and after another four weeks, the dose was increased to 30 mg once daily for the remainder of the trial (16 weeks). In the second pioglitazone hydrochloride treatment group, patients received an initial dose of 15 mg once daily and were titrated to 30 mg once daily and 45 mg once daily in a similar manner. Treatment with pioglitazone hydrochloride, as described, produced statistically significant improvements in HbA1c and FPG at endpoint compared to placebo (see Table 18). 

In a 16 week monotherapy trial, 197 patients with type 2 diabetes were randomized to treatment with 30 mg of pioglitazone or placebo once daily. Therapy with any previous antidiabetic agent was discontinued six weeks prior to the double-blind period. Treatment with 30 mg of pioglitazone produced statistically significant improvements in HbA1c and FPG at endpoint compared to placebo (see Table 19).
The invention relates to a traditional Chinese medicine formula for treating pinkeye. The formula is prepared from the following active ingredients by weight: 300g of balsam pears (150g of dried balsam pears) and 20g of scouring rush. The balsam pears are sliced and the scouring rush is cut into 3-5cm short pieces, the two kinds of ingredients are simultaneously put into an earthen pot, clear water is injected into the earthen pot, the ingredients are decocted with slow fire to obtain two bowels of liquid, the dregs are filtered and the liquid is taken. The formula is taken once in the morning and once in the evening and can relieve pinkeye after being used for one course of treatment (3 days). The balsam pears are cold and bitter and have the functions of removing toxicity and improving the eyesight. The formula has certain curative effects on pinkeye.