MRI with hyperpolarized xenon-129 and helium-3 noble gases has been tested and utilized at CCHMC in adults and children over 4 years with a very strong safety record. The safety of both helium-3 and xenon-129 is well known, with 3He being used safely and effectively to image infants, and 129Xe being used extensively in school-age children at CCHMC and elsewhere. Upon inhalation of an anoxic gas mixture, this mixture mixes with the residual volume of standard oxygenated breathing air that remains in the lung even after a normal exhalation, yielding a hypoxic breath. This is performed in the MRI room with pulse oximetry.

The investigators believe, based upon over 2 decades of experience with these noble gases, that the risk of the procedure is very low and primarily relates to the tidal hypoxic inhalation. (The reason for the hypoxic inhalation is because of the depolarizing effects of O2 on the hyperpolarized gas MRI signal.) Thus, an unpublished pilot feasibility study at Cincinnati Children's Hospital was conducted with infants age 0 - 5 months to provide safety data to expand MRI with gases to the neonatal population by pilot testing a brief hypoxic challenge in a controlled setting. The study was conducted in the NICU and included an induced 3-second apnea breath-hold with inhaled nitrogen (N2) while the infant was closely monitored. The study progressed stepwise beginning with 3 infants on room air, 3 infants on oxygen with nasal cannula, then 3 infants currently requiring respiratory support with CPAP or RAM cannula. After each cohort of 3, there was a safety review by the PI and at least one Sub-I who is also a faculty member in the NICU to determine safety before progressing to the next cohort. Results indicate that the procedure was feasible in these populations and was safe. There were no reportable adverse events. Approximately 20 seconds after the breath-hold, there was an expected small transient decline in SpO2 of approximately 5% which quickly (within 5 seconds) returned to baseline, and there were no significant changes in heartrate during the procedure; importantly, no procedure required stopping due to unexpected values. While this pilot study used ultrapure \~100% N2 to induce an apneic period, the degree of apnea will be similar or significantly less with this protocol's xenon apneic periods (Xe concentrations of \~10-100%, and thus will introduce no apneic differences in the proposed study. Safety for this previous pilot study was based upon the much more rigorous clinically accepted hypoxic challenge used to test "fitness-to-fly" done on older infants and children as well as adults. In one research study, the hypoxia challenge test (HCT) was performed on neonates as young as \<1 week old with diagnoses of prematurity and BPD. The prospective observational study was carried out on 3 groups of infants: healthy term infants at ≤7 days-old, preterm infants (≥34 weeks CGA) without BPD 2 to 3 days before discharge, and preterm infants with BPD. The infants were placed in a body plethysmograph and inhaled 15% O2 while continuously observed for 20 minutes. If the SpO2 dropped to 85%, the test was stopped and was recorded as a test failure. In Group 1 (full term infants), there was 1 failure out of n=24 (4.2% failure rate). In Group 2 (preterm without BPD), there were 12 failures out of n=62 (19.4% failure rate). In Group 3 (preterm with BPD), there were 16 failures out of n=23 (69.6% failure rate). The BPD infants reached the minimum SpO2 earlier (average 8 minutes) than preterm infants without BPD (average 15 min). While the "fitness-to-fly" test is not routinely performed at Cincinnati Children's, and is typically only utilized at altitude, this previous study with lengthy exposure of the hypoxia challenge is significant in the context of this protocol in that the protocol has significantly less risk (3-second apnea period, as opposed to lengthy hypoxia). Even the BPD cohort had exhibited resilience with an average 8 minute window before desaturation to 85% SpO2.