Lipoids and other fat-like substances containing vitamins are separated from animal or vegetable tissues or the crude fluids therefrom by treatment with an alcohol having from six to ten carbon atoms or a mixture thereof.  Room temperature may generally be employed.  Operation in the cold at definite pH values and with exclusion of air is effected for the extraction of readily decomposable substances.  Cellular tissues or juices, from liver, spleen or testicle for example, or blood may be treated.  The process is also applicable for the elimination of the extractive substances, as when therapeutically active solutions of albumen or carbohydrate are being purified.  According to examples: (1) juice expressed from liver and of pH 6.8-7.2 is mixed with octyl alcohol, nitrogen being passed through the mixture for 3--4 hours while cooling with ice. The mixture of lipoids and alcohol which separates in a solid or semi-liquid condition is caught on a filter and the solvent removed by evaporation under reduced pressure.  Nonyl or hexyl alcohol, for example, may be used instead of octyl; (2) blood serum is agitated at room temperature with octyl alcohol.  The mixture of lipoids and alcohol which separates is removed by centrifuging or filtering.  A serum nearly free from lipoids is obtained.  The Specification as open to inspection under Sect. 91 comprises also the use of amyl alcohol for the extraction, or of a fat solvent which is sparingly soluble in water, e.g. aliphatic or aromatic hydrocarbons such as toluene and xylenes, or halogenated hydrocarbons such as chloroform or methylene chloride, with the addition of water-soluble substances of "strong capillary activity" such as soaps, reaction products of ethylene oxide with alcohols, amines or carboxylic acids, or esters of higher fatty acids with sulphonated alcohols described for example in Specifications 366,916, 367,585, and 372,005.  This subject-matter does not appear in the Specification as accepted.ALSO:Lipoids and other substances having the character of fats, which may have a curative value owing to their hormone and vitamin content, are separated from animal or vegetable tissues or the crude fluids therefrom by treatment with an alcohol having from six to ten carbon atoms or a mixture thereof.  Room temperature may generally be employed.  Operation in the cold at definite pH values and with exclusion of air is effected for the extraction of readily decomposable substances.  Cellular tissues or juices, from liver, spleen or testicle for example, or blood may be treated. The process is also applicable for the elimination of the extractive substances, as when therapeutically active solutions of albumen or carbohydrate are being purified.  According to examples: (1) juice expressed from liver and of pH 6.8-7.2 is mixed with octyl alcohol, nitrogen being passed through the mixture for 3-4 hours while cooling with ice.  The mixture of lipoids and alcohol which separates in a solid or semi-liquid condition is caught on a filter and the solvent removed by evaporation under reduced pressure.  Nonyl or hexyl alcohol, for example, may be used instead of octyl; (2) blood serum is agitated at room temperature with octyl alcohol.  The mixture of lipoids and alcohol which separates is removed by centrifuging or filtering.  A serum nearly free from lipoids is obtained.  The Specification as open to inspection under Sect. 91 comprises also the use of amyl alcohol for the extraction, or of a fat solvent which is sparingly soluble in water, e.g. aliphatic or aromatic hydrocarbons such as toluene and xylenes, or halogenated hydrocarbons such as chloroform or methylene chloride, with the addition of water-soluble substances of "strong capillary activity" such as soaps, reaction products of ethylene oxide with alcohols, amines, or carboxylic acids, or esters of higher fatty acids with sulphonated alcohols described for example in Specifications 366,916, 367,585, and 372,005, [all in Group IV]. This subject-matter does not appear in the Specification as accepted.