The mixing device for chemical and/or biological substances, comprises a first drive device (12) for rotating an arm (16) around a first rotating axis (18), a cylindrical mixing container connected in an interval to the first rotating axis with the arm for the reception of the substances, a second drive device for rotating the mixing container around a second rotating axis (28) running through the mixing container, a reception container (24) for the reception of mixing container, and/or a mixing container for a dual asymmetric centrifuge. The mixing device for chemical and/or biological substances, comprises a first drive device (12) for rotating an arm (16) around a first rotating axis (18), a cylindrical mixing container connected in an interval to the first rotating axis with the arm for the reception of the substances, a second drive device for rotating the mixing container around a second rotating axis (28) running through the mixing container, a reception container (24) for the reception of mixing container, and/or a mixing container for a dual asymmetric centrifuge with a first reception area for the reception of a first substance and a second reception area for the reception of a second substance. The internal wall of the mixing container has different intervals to second rotating axis, and/or a holding device arranged within the reception container serves for holding the mixing container in the reception container. An opening is present in a separation wall between the reception areas in order to enable the transportation of one of the substances into another reception area during emerging centrifugal power for mixing single- or multiphase mixture, for cell disruption and for reconditioning of tissues in the dual asymmetric centrifuge. The longitudinal axis of the mixing container is arranged in an angle to second rotation axis and the angle is 70-110[deg] . The longitudinal axis of the reception container collapses with the second rotating axis. The mixing container is pluggable in the reception area of the holding device. The internal dimension of the reception area corresponds to the external dimension of the mixing container. The reception area is lockable with a cover that has an opening for guiding a mixing container-neck. The mixing device comprises a ring-shaped damping element that lies on the mixing container. The holding device is formed as reception container-insert, comprises reception areas, where the longitudinal axis of the reception area passes parallel to second rotating axis and in an interval to the rotating axis, and/or reception areas, where the longitudinal axis of the reception areas passes vertically to the second rotating axis. The opening is arranged on a longitudinal axis of the first and/or second reception area in the mixing container. The second reception area is arranged within the first reception area and the second reception area is formed in cone-shaped manner, where the opening is arranged on the cone top, and/or the first reception area is formed in cylindrical manner. Independent claims are included for: (1) a method for tissue reconditioning and for disruption of cells; and (2) a kit for tissue reconditioning and for disruption of cells.