Nutritional formulas are commonly consumed by individuals both to supplement their food intake and as total meal replacers. Consumption rates of nutritional formulas are increasing also in Asia. Very little is known about differences in the glycaemic response to nutritional formulas between different ethnic groups. The aim of the current study is to determine the glycaemic response (GR) and glycaemic index (GI) to three nutrition formulas in Chinese, Indian and Malay subjects.

The study will have a randomised, non-blind, between-subjects, crossover design. The subjects will return for six test sessions. At three random sessions subjects will test the reference food and on the remaining three sessions they will consume the test foods (Diasip, Isocal, Protinex). The first test session will be a glucose test session (for subjects requiring glucose tests). The reference food will be anhydrous glucose (50 g) dissolved in 250 ml of water. The test foods will be portions of the nutritional formulas containing 50 g of available carbohydrates. Subjects who have previously taken part in GI studies at the CNRC and who have already completed three reference glucose tests will have to complete only the three test food sessions. However, they may be asked to do one, two or all three of the glucose tests if their previous test results are old (\> 3 months) or are not within the required precision (CV \<30%). This aspect will be at the discretion of the investigator and communicated to the participant at the time of briefing (before consenting).

The primary measurement in the study will be the glycaemic response. This will be measured using finger-prick blood samples. The fingers in the non-dominant hand will be used. To encourage blood flow the hand will be warmed using warm water or a hot towel beforehand if required. The finger will then be disinfected using a sterile wipe. Blood will be obtained by finger prick using a single-use lancing device. To minimise plasma dilution fingertips will not be squeezed to extract blood but gently massaged starting from the base of the hand moving towards the tips. The first two drops of expressed blood will be discarded and the next drop (5 μL) will be used for testing. Blood glucose in the sample will be measured using the HemoCue® 201+ Glucose analyser (HemoCue Ltd, Dronfield, UK).

The participants will arrive at the laboratory between 8:30-9 am following a 10 hour overnight fast. They will be instructed not to partake in intense level sports and avoid alcohol consumption on the evening prior to a test session. Following a 10 minute rest two blood samples will be obtained five minutes apart for determining baseline blood glucose levels. If the two baseline blood glucose values are far apart a further 1-2 blood samples may be taken until two stable values are obtained. The study session will be terminated if stable values cannot be obtained or if baseline blood glucose levels are above 6 mmol/L. After obtaining acceptable baseline blood glucose values they will be given either the standard or test food to consume. They will be asked to consume the drink at a comfortable pace within 10 minutes. Further blood samples will be obtained at 15, 30, 45, 60, 90, 120, 150 and 180 minutes for blood glucose measurements. Mandatory sampling will be carried out up to 120 minutes. If the blood glucose level has not reached baseline levels by the end of 120 minutes further samples may be taken at 150 and 180 minutes. During the entire testing period the participants will be instructed to remain rested and in the laboratory.