anthonyyazdaniml/gliner-biomed-curated-corpus huggingface dataset:
Sequential Blood Loss: Blood loss will be simulated by withdrawal of 20% of estimated total blood volume. Estimated total blood volume for men = 75 mL/kg and for women = 65 mL/kg. The calculated target withdrawal volume is also consistent with an allowable estimated blood loss equation assuming a starting hematocrit (hct) of approximately 45% in males and 40% in females, where the expected drop in hct for this volume withdrawn is 7 percentage points in males and 6 percentage points in females. An actual drop in hct may not be observed in this study due to the relatively expedient removal of blood and no simultaneous administration of fluids (i.e., no "hemodilution" with crystalloid). Blood will be removed in aliquots up to \~333 mL. For safety, men will have no more than 4 units of blood removed (1,333 mL) and women will have no more than 3 units of blood removed (1,000 mL).

The blood will be withdrawn via the venous catheter and we anticipate that it will take 15-20 min for each stage of blood withdrawal to occur. A total of 3 aliquots will be withdrawn over approximately sixty minutes in women and 4 aliquots withdrawn over 80 minutes in men. The blood will be stored using preservatives and anticoagulation procedures similar to those used for perioperative hemodilution and/or intraoperative cell saver. The blood will remain in the study room until it is re-infused. We will use standard "auto-transfusion" blood collection equipment identical to that used in the ORs for perioperative hemodilution techniques. The blood will be drained by gravity from an indwelling vascular catheter into anti-coagulant preservative solution using tubing and bags systems provided by the autologous transfusion team that we routinely work with in the ORs. For the first several studies we will schedule them at a time when auto-transfusion OR personnel can be present and after that the anesthesiologists will be directly involved in drawing the blood, subsequent handling and reinfusion. The blood will never leave the autologous transfusion bag and a closed system will be used. The blood will be held at room temp with gentle agitation for \~60-80 minutes and then re-infused over 60-80 minutes via a large bore IV; monitoring will continue during reinfusion. Following completion of the reinfusion subjects will de-instrumented and observed in the physiology lab for two hours.
Diet therapy for hyperkalemia in people with chronic kidney disease (CKD) has shifted considerably in recent years with the observations that reported potassium intake is weakly, or not at all, associated with plasma potassium levels in this population. One of the lingering debates is whether dietary potassium presents a risk of hyperkalemia in the postprandial state. Although there is general agreement about the need for additional research, the commentary by Varshney et al contends that the available research sufficiently demonstrates that high-potassium plant foods do not pose a risk of postprandial hyperkalemia. Others argue that this remains unsettled science. Although the traditional approach of providing people with CKD lists of high-potassium foods to limit or avoid may be unnecessary, those at high risk of hyperkalemia should be encouraged to consume balanced meals and control portions, at least until some of the key research gaps in this area are resolved. This editorial critiques the analyses offered by Varshney et al and explains the rationale for a more cautious approach to care.
FIELD: medicine. SUBSTANCE: diketene-imine derivatives having formula (I) is used as reagent 1, methylamine being used as reagent 2. Their interaction is carried out in the medium of solvent at room temperature. Methylamine is used in gaseous state or in aqueous solution, preferred quality of said solution being 10 moles of 40 mass % solution. EFFECT: improves efficiency of the method. 3 cl
1,205,094. Salt of zinc chelate of diethylenetriaminepenta acetic acid. COOPER LABORATORIES Inc. 20 Nov., 1968 [21 Nov., 1967], No. 55029/68. Addition to 1,152,650. Heading C2C. Novel trisodium or tripotassium salts of the zinc chelate of diethylenetriaminepenta-acetic acid and hydrates thereof are prepared by reaction of the alkali metal hydroxide, the diethylenetriaminepenta-acetic acid and zinc acetate in the appropriate proportions followed by crystallization. Pharmaceutical compositions in conventional forms for oral or parenteral administration and used for treating hypertension comprise an above novel compound and a carrier or diluent therefor.
A powdery mildew (Pm) resistance locus PmRc1 was identified and transferred from Roegneria ciliaris into wheat. Two compensative translocation lines carrying PmRc1 were developed. Powdery mildew (Pm), caused by the biotrophic fungal pathogen Blumeria graminis f.sp. tritici (Bgt), is a global destructive disease of bread wheat (Triticum aestivum L.). Identifying and utilizing new Pm resistance gene(s) is the most fundamental work for disease control. Roegneria ciliaris (2n = 4 x= 28, genome ScScYcYc) is a wild relative species of cultivated wheat. In this work, we evaluated wheat-R. ciliaris disomic chromosome addition lines for Pm resistance in multiple years. The introduction of R. ciliaris chromosome 1Sc into wheat enhanced resistance. The resistance locus on 1Sc was designated as PmRc1. To cytologically map PmRc1, we induced structural rearrangements using ion irradiation and increasing homoeologous chromosomal recombination. The identified 43 1Sc translocation or deletion lines were used to construct 1Sc cytological bin map by marker analysis using 111 molecular markers. Based on the Pm resistance of the characterized structural rearrangement lines, the PmRc1 locus was cytologically mapped to bin 1ScS-8 of 1Sc short arm, flanked by markers CMH93-2 and CMH114-1. Two compensatory chromosomal translocation lines (T1ScS · 1BL and T1ScS-1AS · 1AL) carrying PmRc1 were developed and assessed for their agronomic traits. Translocation chromosome T1ScS · 1BL had enhanced Pm resistance accompanied by negative effects on grain number and single plant yield. Translocation chromosome T1ScS-1AS · 1AL had enhanced Pm resistance and increased spikelet number per spike, without any obvious negative effect on other tested traits. Thus, T1ScS-1AS · 1AL is recommended preferentially used in wheat breeding for Pm resistance.
Gestational diabetes mellitus (GDM) is associated with increased inflammation in adipose tissues. Fibroblast growth factor 21 (FGF21) is an endocrine hormone which signals to multiple tissues to regulate metabolism. However, its role in GDM remains largely unknown. In this study, we found that impaired FGF21 signaling in GDM correlates with worsened inflammation and insulin resistance in white adipose tissues in mice. Mechanistically, the pregnancy-related upregulation of FGF21 signaling in adipocytes promotes the differentiation of regulatory T cells (Tregs), which are critical for reducing pregnancy-induced adipose tissue inflammation. The anti-inflammatory effects of FGF21 may involve linolenic acid-mediated PGE2 synthesis in adipocytes. These findings underscore FGF21's role in mediating crosstalk between mature adipocytes and immune cells in white adipose tissue and suggest that targeting FGF21 signaling and its downstream metabolites could offer a potential therapeutic approach for GDM in humans.
OBJECTIVES: Ascites, often associated with critical pathologies such as liver cirrhosis or bowel perforation, can be complicated by fungal infection, increasing mortality especially in intensive care settings and demanding rapid diagnosis and adequate treatment. Traditional microbiological diagnostic methods have limited sensitivity in accurately identifying fungal pathogens in ascitic fluid. Alternative diagnostic methods may offer important insights to enable guiding of antifungal therapy and refining empirical treatment strategies. The objective of this study was to evaluate the potential of next-generation sequencing methods to identify specific fungal pathogens responsible for ascitic fluid infections.
METHODS: We prospectively collected 50 ascitic fluid samples from ICU patients with suspected ascites infection. In addition to standard culture-based microbiological testing, an ascitic fluid aliquot underwent fungal DNA isolation and was analyzed by next-generation sequencing (NGS) methods for identification of fungal species.
RESULTS: Of 50 ascitic samples collected, five samples showed growth of Candida spp. in culture. After DNA isolation and ITS2 PCR, detectable amplification was achieved in 10 samples. Sequencing of the 50 patients' samples identified facultative pathogenic fungi in 19 patients. In 15 cases, culture alone would not have permitted the identification of all facultative pathogenic fungi. The identification of fungal DNA by sequencing was significantly associated with poor patient outcome and a number of clinical parameters.
CONCLUSIONS: Our results show a higher sensitivity for NGS-based diagnostic methods in the identification of ascitic fluid fungal infections compared to culture-based diagnostics. This may be beneficial especially for patients in a critical care setting, who have an increased prevalence of comorbidities and high mortality. The implementation of such methods in standard diagnosis will require increased standardization of the workflows and interpretation of the sequencing results with respect to patients' clinical picture.
Blood stream infection with Microbacterium species in humans is rare and frequently linked to the presence of immunosuppressed conditions such as patients on chemotherapy or corticosteroids. Presence of indwelling catheters is also a potential risk factor for M. aurum infection. No case report has been documented in the literature regarding the pathogenic potential of M. aurum in causing bacteremia. This is the first case series reporting bacteremia by M. aurum describing the risk factors and sensitivity pattern of this pathogen. In this case series, we have described bacteremia caused by M. aurum. The risk factors and sensitivity pattern of this pathogen have also been evaluated. Here, we describe the clinical course and presentation of three patients whose blood culture showed growth of M. aurum. Indwelling venous catheter for hemodialysis or for chemotherapy for the treatment of acute lymphoblastic leukemia was found to be a risk factor in two patients. Rheumatoid arthritis was the underlying condition in the second patient and was started on immunosuppressants. Blood samples were collected during the febrile period. The blood culture samples of all these patients had pure isolates of M. aurum, identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. All three patients were managed according to the sensitivity reports and were discharged in stable condition.
The study group is applied with Guardix-SP after Lumbar discectomy or laminectomy. Afterwards, they visit at 2 weeks, 12 week to conduct examinations and assess Questionnaires.
FIELD: medicine, neurology, neurosurgery. SUBSTANCE: for anesthesia carrying out lymphonodus is punctured and medicinal mixture is administrated into lymphonodus at the rate 0.1-0.3 ml/min. Medicinal mixture has: 0.01-% solution of clophelin at the dose 1 mcg/kg, 0.25-% solution of isoptin at the dose 0.07 mg/kg and 1 ml of 2-% lidocaine. Invention is used for treatment of persistent pain syndromes caused by the damage of nervous structures (neurogenic pain syndromes). Method ensures to arrest chronic pain syndromes, ameliorate tissue traumatization at administration of drugs into lymphatic system. EFFECT: enhanced effectiveness of method. 2 ex
The demographic and clinical characteristics of all subjects willing to participate in the study will be questioned with the "Sociodemographic Data Form". Sociodemographic data form; pain, age, gender, height, weight, type of rheumatism, how many years ago the patient was diagnosed, the presence of rheumatism and scoliosis in the family, the drugs used, exercise habits, exacerbation history, whether they used a device before, and the awareness of the family about scoliosis. The trunk rotation angles with the forward bend test and the Cobb angle with the SubrotoAngleAid application via x-ray. Respiratory functions will be evaluated with portable spirometer, body awareness will be evaluated with Walter Reed Visual Assessment Scale, and quality of life will be evaluated with Scoliosis Research Association Scale-22 (SRS-22). After the evaluations, in the first treatment session in the clinic, the cases in the first group; Body awareness, respiratory control, differences between rotational and normal breathing, posture exercises and core stabilization exercises will be taught and family training will be provided. In the next session, a home program will be created by applying 3D scoliosis exercises that are structured according to the needs of the patients. Body awareness, respiratory control, posture exercises and core stabilization exercises will be taught and family training will be given to the patients in the second group in the first treatment session. In the next session, conventional exercises for the needs of the cases will be taught and a home exercise program will be created. Both groups will be interviewed for 2-week periods using the WTE method, and the exercises will be progressed gradually as needed. At the beginning of the treatment and in the 6th month, all evaluations will be repeated and the effectiveness of the exercises will be investigated. SPSS (Statistical Package for Social Sciences) (SPSS 21.0) statistical program will be used in the statistical analysis of the data. In all statistical analyzes, p \<0.05 will be considered significant.
Halitosis in dogs is an initial indication of periodontitis, highlighting its significance as a vital marker for underlying problems. Moreover, the oral microbial population has a significant influence on periodontal disease. Measuring the oral microbiota may be used in addition to breath odor, dental plaque, and gingivitis scoring to assess the impact of dental chews on oral health. In this study, we aimed to determine the differences in breath odor, oral health outcomes, and oral microbiota of adult dogs consuming a novel dental chew compared with control dogs consuming only a diet. Twelve healthy adult female beagle dogs were used in a crossover design study. Treatments (n = 12/group) included: diet only (control) or the diet + a novel dental chew. Each day, one chew was provided 4 h after mealtime. On days 1, 7, 14, 21, and 27, breath samples were analyzed for total volatile sulfur compound concentrations using a halimeter. On day 0 of each period, teeth were cleaned by a veterinary dentist blinded to treatments. Teeth were scored for plaque, calculus, and gingivitis by the same veterinary dentist on day 28 of each period. After scoring, subgingival and supragingival plaque samples were collected for microbiota analysis using Illumina MiSeq. All data were analyzed using SAS (version 9.4) using the Mixed Models procedure, with P < 0.05 being significant. Overall, the dental chews were well accepted. Dogs consuming the dental chews had lower calculus coverage, thickness, and scores, lower gingivitis scores, and less pocket bleeding than control dogs. Breath volatile sulfur compounds were lower in dogs consuming the dental chews. Bacterial alpha-diversity analysis demonstrated that control dogs had higher bacterial richness than dogs fed dental chews. Bacterial beta-diversity analysis demonstrated that samples clustered based on treatment. In subgingival and supragingival plaque, control dogs had higher relative abundances of potentially pathogenic bacteria (Pelistega, Desulfovibrio, Desulfomicrobium, Fretibacterium, Helcococcus, and Treponema) and lower relative abundances of genera associated with oral health (Neisseria, Actinomyces, and Corynebacterium). Our results suggest that the dental chew tested in this study may aid in reducing periodontal disease risk in dogs by beneficially shifting the microbiota population and inhabiting plaque buildup.
PURPOSE:To make it possible to measure a HBc antibody with high sensitivity, by utilizing recominant DNA technique to develop a HBc antigen in yeast and using the yeast producing HBc antigen. CONSTITUTION:A yeast producing HBc antigen is produced and accumulated by culturing a recombinant, that is, a yeast fungus, of which the character is transformed by recombinant DNA technique so as to produce a HBc antigen, in a proper culture medium. Yeast cells are centrifugally separated from the culture medium to be ground in a buffer solution and applied to low speed centrifugal separation to obtain a HBc antigen stock material. An acid is added to this stock material to transfer the pH thereof to an acidic side and lipids or impurity protein are removed as a precipitate. Thereafter, ion exchange chromatography and density gradient centrifugal separation are successively performed to make it possible to highly purify the HBc antigen. The measurement of a HBc antibody is enabled by a PHA method, an EIA method or a RIA method using thus obtained HBc antigen.
The present invention involves an automated pill compounding system, and associated processes and methods, for compounding encapsulated medications as prescribed for individual patients. The invention involves adaptation of micro-dosing technology, which enables sufficiently small and precise amounts of drug API or formulation to be reliably dispensed into capsules while avoiding need for non-therapeutic or non-functional excipient. Utilizing such capability to perform compounding requires relevant systems configured and applied to receive and execute patient-specific information in discrete runs. The invention combines the principle of custom compounding with principles of precision capsule micro-dosing/filling. Benefits to facilitating personalized medicine include expanding usage of polypills and the resultant reductions in medication administration and compliance errors, as well as enhancing dosage options to better account for patient demographics, condition severity, genetic profile, etc. Another advantage is improved availability of drugs whose mass-produced versions may be limited. Compliance packaging can also be enhanced for high-pill-burden patients.
FIELD: food industry. ^ SUBSTANCE: invention relates to a technology of deer farming products processing, in particular, to methods for production of biologically active products from complex raw material i.e. velvet antlers, tails and genitals. The method envisages grinding raw material i.e. maral buck velvet antlers, tails and genitals taken at a weight ratio of 60:20:20. The one performs extraction with 50% alcoholic solution acidated up to pH 4.7, the ratio of alcohol to raw material 7:1 during 12-14 days at a temperature of 20-25C. One performs stirring for 5 minutes on a daily basis with subsequent extract freezing-out at a temperature of - 15 - (-18)C during 5 days. Then the end product is filtered. ^ EFFECT: invention allows production of a highly effective finished product and more complete and rational usage of velvet antler deer raw material, in particular, maral tails and genitals. ^ 6 tbl, 1 ex
Histological analysis with 2,3,5-triphenyltetrazolium chloride (TTC) staining is the most frequently used tool to detect myocardial ischemia/reperfusion injury. However, its practicality is often challenged by poor image quality in gross histology, leading to an equivocal infarct-boundary delineation and potentially compromised measurement accuracy. Here, we introduce several crucial refinements in staining protocol and sample processing, which enable TTC images to be analyzed with light microscopy. The refined protocol involves a two-step TTC staining process (perfusion and immersion) and subsequent Zamboni fixation to differentiate myocardial viability and necrosis, and use of Coomassie brilliant blue to label area-at-risk. After the duo-staining steps were completed, the heart sample was embedded and sliced transversally by a cryostat into a series of thin sections (50 µm) for microscopic analysis. The refined TTC (redTTC) assay yielded remarkably high-quality images with striking color intensity and sharply defined boundaries, permitting unambiguous and reliable delineation of the infarct and area-at-risk. In the same animals, the redTTC assay showed good agreement with the in-vivo gold standard measurements (LGE and MEMRI). Meanwhile, redTTC imaging allows tracking of viable cardiomyocytes at cellular resolution, and with this enhanced capability, we convincingly demonstrated the pro-survival action of stem cells based-therapy. Therefore, the redTTC assay represents a significant technical advance that permits precise detection of the true extent of cardiac injury and cardiomyocyte viability. This approach is cost-effective and may be adapted for use in diverse applications, making it highly appealing to many laboratories performing ischemia/reperfusion injury experiments.
The invention discloses a Chinese herb extract. The Chinese herb extract is prepared from 4-5 parts of hop, 6-8 parts of aloe, 1-2 parts of soapberry, 2-2.5 parts of avena sativa kernel, 1-2 parts of melissa and 1-1.5 parts of mint leaf. The invention further discloses a preparing method and application of the Chinese herb extract, a traditional Chinese medicine hair conditioner using the Chinese herb extract, and a preparing method of the traditional Chinese medicine hair conditioner. The Chinese herb extract can achieve bacteriostasis and diminish inflammation, clear away heat and toxic materials and resist skin allergy, and is rich in nutrient. The Chinese herb extract can be used in cosmetics, especially hair products. The traditional Chinese medicine hair conditioner has a good cleaning effect, and can promote the circulation of scalp blood, repair chaff scale and cuticle, make hair quality better and prevent hair loss.
CG will be practicing only one specific pattern of step isometric contractions (SPSIC) scheme. It means that 90 trials in all training sessions will consist only of SPSIC 1.
<P>PROBLEM TO BE SOLVED: To provide a TRPA1 activity inhibitor effectively inhibiting activation of TRPA1, a TRPA1 activity-inhibiting method effectively inhibiting activation of TRPA1, and a skin care preparation for external use of low irritating nature to the skin.  <P>SOLUTION: The TRPA1 activity inhibitor for inhibiting TRPA1 activity comprises a carbonate ion and/or a bicarbonate ion. The TRPA1 activity inhibitor for inhibiting TRPA1 activity comprises a substance that dissociates into a carbonate ion and/or a bicarbonate ion. The TRPA1 activity-inhibiting method for inhibiting TRPA1 activity comprises bringing a carbonate ion and/or a bicarbonate ion into contact with TRPA1. The skin care preparation for external use contains ammonia and further comprises a substance that dissociates into a carbonate ion and/or a bicarbonate ion.  <P>COPYRIGHT: (C)2011,JPO&INPIT