anthonyyazdaniml/gliner-biomed-curated-corpus huggingface dataset:
FIELD: medicine.SUBSTANCE: invention refers to medicine, and can be used for producing cytokines from umbilical blood thrombocytes. Method involves three stages: at the first stage, umbilical blood is sampled from different donors, Stabisol solution is administered in each packet with donor blood in amount of 20 % of the blood volume of the packet, each blood pack is centrifuged in a smooth program change mode, as a result of which blood is separated into thrombocyte enriched plasma and erythrocytes. Said program consists of the following modes: acceleration to 540 RCF for 5 minutes; acceleration to 900 RCF for 2 minutes; braking to 300 RCF and rotation for 2 minutes; braking up to 100 RCF and rotation for 1.3 minutes with subsequent disconnection of the centrifuge, then removing the erythrocytes from the packs and recycling them. At the second stage, all donor OTP packets are combined and all thrombocytes are concentrated, for this purpose the produced thrombocyte enriched plasma is transferred into the appropriate number of 50 ml-type falcon test tubes, centrifugation is carried out for concentration of thrombocytes in mode of 15 minutes at rate of 4000g, as a result of centrifugation a platelet concentrate and lean plasma are obtained. Oblong plasma is taken for use as a solvent, leaving 5 ml of plasma at the bottom of the test tubes, obtained thrombocyte concentrates of different donors are combined in one test tube with volume of 50 ml and diluted with depleted plasma to volume 50 ml or the solvent used is normal saline, if further plasma proteins need to be excluded; obtained thrombocyte concentrate pool is re-centrifuged in 60-minute mode at rate of 50g for removal of erythrocyte and leukocyte residues. After centrifugation, the supernatant fluid in amount of 45–48 ml is transferred into new 50 ml test tube, and 1 ml of the solution is sent to a laboratory for platelet count on an automated haematological analyzer. At the third stage, the obtained thrombocyte concentrate with a known amount of thrombocytes per ml or mcl is subjected to a storage/activation procedure, wherein the falcon with thrombocyte concentrate is lowered into a cryogenic storage of a dry type for 15 minutes at a temperature of -196 degrees Celsius, frozen product is stored in a freezing chamber at a temperature of -80 degrees Celsius, where it is stored for up to 3 years. Further activation of the product is carried out before immediate use by rapid thawing, for this purpose the falcon is placed in a water bath with a temperature of +37 degrees Celsius until complete thawing of the product, and if necessary to obtain a product with concentration of cytokines/thrombocytes less than in a frozen product, the end product is dissolved in the corresponding number of times, wherein the initial concentration of thrombocytes is used.EFFECT: disclosed method allows to prepare a cocktail of cytokines for its further use as a substrate for preparing medicinal preparations, the method is economical and enables to produce a product with cytokine concentration of up to 1 million/mcl.1 cl
Fluid resuscitation is the most effective treatment of shock. Isotonic crystalloid solution is the current recommended initial fluid resuscitation. However, this kind of fluid has high volume of distribution and may require large volume administration before achieve therapeutic goal of shock reversal. There are rising concern about the delay in shock reversal and adverse consequences of large amount volume of fluid therapy. Colloid fluid have been used as the alternate fluid resuscitation, aiming to limit the volume of fluid resuscitation and promote shock reversal. Data from a recent randomized controlled study showed the improve long term survival among shock patients whose resuscitated with colloid solution. There were evidence about the increase incidence of acute kidney injury among critically ill patients who received hydroxyethyl starch, a previously worldwide used colloid solution. For septic shock, the leading cause of shock in current situation, resuscitation with albumin may associated with better outcome, while increasing mortality had been reported among the patient who received hydroxyethyl starch. Whether colloid infusion can improve shock reversal rate and decrease complication associated with fluid resuscitation, had inconclusive information.
Materials and methods: This is a single-blind superiority randomized controlled clinical trial. Women aged over 18 years, with breast cancer in stages I to IIIC and who have an indication for curative treatment with neoadjuvant or adjuvant chemotherapy at HCIII/INCA will be eligible. Patients with a previous diagnosis of another primary cancer will be excluded; patients undergoing surgery and/or chemotherapy at another institution; patients who previously had altered sensitivity in the feet; patients who are unable to answer the questionnaires and patients unable to receive photobiomodulation due to acute infections in the lower limbs. After recruitment, the women will be allocated into three groups: two Intervention groups (use of the IV and V LED board; and IV, V and Violet) and a Control group (use of the LED board without light emission). Peripheral neuropathy, pain, global muscle strength, body balance, sensitivity, health-related quality of life and related independent variables will be evaluated, such as the characteristics of the patient, the tumor and the oncological treatment performed. Data analysis: Statistical analysis will be conducted following intention-to-treat principles. Data normality will be tested using the Kolmogorov Smirnov test, considering as normal distribution those with p \> 0.05.
BACKGROUND: Chronic obstructive pulmonary disease (COPD) affects many patients across the United States. Morbidity related to COPD can lead to increased financial strain to health care system. The United States is also shifting toward value-based payments, which rely on satisfying quality measures. Pharmacists are equipped with knowledge in adjusting medications based on symptom burden and guideline recommendations in COPD and are equipped with the proper knowledge to address quality measures.
OBJECTIVE: This project aimed to determine the impact of a clinical pharmacy service centered around inhaler education and optimization on COPD morbidity and Uniform Data System (UDS) quality measure satisfaction in a federally qualified health center.
METHODS: This quality improvement project consisted of patient referrals by and reports from a population health software for the pharmacy service from November 2022 to March 2023. The outcomes in this study included symptom change measured by follow-up modified Medical Research Council (mMRC) Dyspnea Scale in addition to changes in compliance with UDS quality measures. At follow-up, patients were administered another mMRC to evaluate treatment effect and determine quality measure satisfaction.
RESULTS: Thirteen patient visits were conducted. Most patients were female (84.6%) with an exacerbation in the previous year (46.1%). All patients received an adjustment in their pharmacotherapy along with inhaler education. The average baseline mMRC score decreased from 2.1 to 0.6, indicating a decrease in overall COPD symptoms. Five quality measures of 13 were satisfied during the follow-up period.
CONCLUSION: The COPD clinical pharmacy service led to an increase in guideline-driven pharmacotherapy regimens for patients with COPD while having an overall decrease in morbidity. Quality measures were also addressed and satisfied after the appointment. Continuation of this quality improvement service will ensure proper assessment of COPD along with addressing UDS quality measures.
Eighteen healthy elderly people, who were classified into two age groups, participated in this study. All participants had not practiced yoga before and were asked not to perform any sports activities while the research was ongoing. In the experimental group, participants (n = 18) had to participate in 10 weeks of yoga classes. In the control group, participants (n = 15) did not perform any exercises or other changes in their daily living life. All experimental group subjects participated in Himalayan yoga classes, which lasted 10 weeks: 2 times per week, 90 min per session. Yoga classes were conducted by 16-year-old qualified yoga instructor from Yoga Academy, Kaunas.
It is challenging to distinguish embryos with a balanced translocation karyotype from a normal karyotype by existing conventional genetic testing methods. However, in germ-cell gamete generation, chromosome exchange and separation through cell meiosis form a different proportion of unbalanced gametes. Adverse birth events may occur, such as repeated miscarriages and fetal birth defects. In this study, the exact breakpoints of structural variation (SV) from two balanced translocation carrier families by using Nanopore long reads sequencing technology were obtained, and haplotype analysis and Sanger verified the accuracy of the detection results, confirming the application value of the Nanopore sequencing technology in the detection of balanced translocation before embryo implantation. Nanopore long-read sequencing was performed to find the precise breakpoint of chromosome-balanced translocation carriers. The breakpoints were subsequently verified by designing primers across the breakpoints and Sanger sequencing. Haplotype linkage analysis of SNPs which can be linked by a read block of families around the breakpoint regions was followed. After frozen (-thawed) embryo transfer (FET), prenatal cytogenetic analysis of amniotic fluid cells confirmed the predicted karyotypes from the transferred embryos. The presence of breakpoints was detected in three embryos of patient 1. No breakpoints were detected in either embryo of patient 2. One balanced translocated embryo from patient 1 and one normal euploid embryo from patient 2 were transplanted back into the patients, and amniotic fluid cells were analyzed for the karyotype of fetuses. The results were entirely consistent with the fetal karyotype. And through late follow-up, both patients successfully had a live birth fetus. The breakpoint location of the balanced chromosome translocation can be accurately found by Nanopore sequencing. The haplotype of carriers can be successfully constructed by Nanopore and sanger sequencing confirmed that the results were accurate. This is very advantageous for preimplantation genetic testing for chromosomal structural rearrangements (PGT-SR) detection in the families without proband.
The invention relates to the field of medical intermediates and antitumor drugs, in particular to a nitrogen mustard-containing terpyridine ligand and a preparation method and application thereof. N, N-di (2-hydroxyethyl) -aniline is used as a raw material, an aromatic aldehyde intermediate of nitrogen-containing mustard is prepared through Vilsmeier-Haack reaction, and then the aromatic aldehyde intermediate is further reacted with 2-acetylpyridine to prepare a terpyridine ligand L1 of the nitrogen-containing mustard. The method is simple and easy to implement, has low requirements on equipment, and is easy to separate and purify the target compound. The compound has good symmetry in structure, and is the terpyridine derivative which has the simplest structure and contains aromatic nitrogen mustard at present. Can be used as ligands of various metal ions to design various metal complexes containing nitrogen mustard. In-vitro anti-tumor experiments show that the compound has excellent anti-tumor effect, can effectively inhibit the growth, migration, crawling, cloning and the like of tumor cells, and has wide research and development values in the field of anti-tumor drugs.
The present randomized controlled clinical trial was conducted to evaluate the effects of Lactobacillus reuteri-containing probiotic suspension as an adjunct to scaling and root planing (SRP).

Material and Methods: Twenty sites from twelve chronic periodontitis patients were recruited and monitored clinically and microbiologically at baseline, three, and six months after therapy for plaque index (PI), bleeding on probing (BOP), probing pocket depth (PPD) and clinical attachment level (CAL) and microbiologically or Porphyromonas gingivalis (P. gingivalis) load. Patients meeting the inclusion criteria were scheduled within one week for two sessions of SRP performed using ultrasonic scalers and hand instrumentation. After SRP, oral hygiene measures were reassured and then sites were randomly allocated to one of the two groups 10 sites each. group I (SRP only) and group II (SRP + probiotic). Group II received SRP and sub-gingival delivery of 1ml of probiotic L. reuteri suspension at baseline (immediately after SRP), one, two and four weeks using blunt syringe. Periodontal dressing was applied after placement of the drug.
This study is a pilot randomized control trial that aims to (a) to determine the differences in self-reported and proxy-reported pain and fear during IV insertion between the interactive VR intervention and control group, and (b) to assess the feasibility (safety, acceptability) of the VR intervention to children/families and the healthcare team in the pediatric emergency department. The study will consist of two study arms, where both study arms will be screened and recruited using the same procedure. Participants will be randomized into either (1) Control group: child life specialist plays an age-appropriate video on a tablet device, or (2) Intervention (VR Distraction): child life specialist facilitates immersive VR experience. Both the intervention and control groups will receive standard medical care (e.g., topical anesthetics). A convenience sample of 80 children and adolescents with cancer (40 participants per treatment arm; 20 boys and 20 girls per treatment arm) will be recruited. In addition to usual care, children in the experimental condition will wear the VR headset plus headphones. In the control condition, children will watch a video (i.e., an age-appropriate video selected by an emergency department affiliated child life specialist) on an iPad, while wearing the same headphones as in the experimental condition. Implementation outcomes include accrual and retention rates, acceptability and technical difficulties. Effectiveness outcomes include child pain, distress, and fear, as well as parent distress.
Methods of counting and searching for surgical sponges with a surgical sponge management system. Duplicate sponge candidates are identified based on a unique identifier from an RFID tag, and a received signal strength indicator (RSSI) value. The RSSI value from the second detection may be compared against an RSSI threshold. If the RSSI value meets or is above the RSSI threshold, a duplicate sponge notification is presented on a display interface. The RSSI threshold may be a fixed value, or based on a sponge type or a tag type. A scan-specific RSSI threshold may be based on the RSSI value and/or an emitted frequency associated with a first detection of the sponge being counted out. Methods disclosed herein may provide for excluding a subset of two or more of the surgical sponges from a search of surgical sponge with an RFID scanner.
Biological systems allow template-directed synthesis of α-peptides. The present invention enables a system that allows template-directed synthesis of other types of polymers as well as α-peptides. The present invention relates to templated molecules and templated molecules linked to a predetermined template. The templated molecules comprise a sequence of functional groups that are linked together. Each functional group is initially linked to an element capable of complementing a predetermined coding element of the template. Following complementation of a coding element, or complementation of a plurality of coding elements, the appended functional groups are linked and the templated moleculed is formed.
This study is a prospective, randomized, single-center clinical trial. The study will be conducted in two Phases. In Phase I, subjects will be assigned to one of two treatment groups. One study group will receive one dual depth Ultherapy treatment to a region on the cheeks, and one study group will receive two dual depth Ultherapy treatments to a region on the cheeks 14 days apart. Average pain scores will be obtained using a Numeric Rating Scale following each region of treatment and for each depth of treatment. In Phase II, based on observations of last follow-up visit in Phase I (at 90 days after treatment), subjects will be given the option to receive treatment of the affected area(s) for the protocol specified region, treated at the treatment depth showing the most efficacy in Phase I. Efficacy will be determined by improvement in Erythematotelangiectatic Rosacea in the treatment area versus control (an untreated area) as determined by primary investigator assessment at the last follow-up visit in Phase I.
Microspheres labeled with a dye for which the absorption spectra is known permit the measurement of blood flows, particularly regional myocardial blood flow (RMBF), without radiation safety concerns and radioactive waste disposal problems. Polystyrene spheres, nominally of diameter 15±0.1 [SD] um and density 1.09 g/ml, are dyed with a fixed quantity, nominally 30 picograms per microsphere, with one of several, nominally 5, colored dyes. The colored microspheres (CM) are injected into the left atrium or into a coronary perfusion. The CM are extracted from myocardium and blood by digestion with KOH and subsequent microfiltration. The dyes are then recovered from the CM within a defined volume of a solvent, and their concentrations are determined by spectrophotometry. The composite absorbance or emission spectra of several dyes are mathematically resolved using matrix inversion. Leaching of dye from the CM was less than 0.1% over a two months period in vitro, and did not occur during 8 hours in blood and myocardium of 4 anesthetized dogs in vivo. For further validation of this method, pairs of CM and RM (15.5±0.1 [SD] um) were simultaneously injected under 5 different RMBF conditions (range: 0-10 ml/(min g)) into the left anterior descending coronary artery of 4 anesthetized pigs, with coronary inflow as a flow-reference, or into the left atrium of 4 anesthetized dogs using aortic blood withdrawal as a reference.
The procedure would be performed with a linear echoendoscope using a 10mm x 10mm or a 15 x 10mm stent. The distal flange of the stent would be deployed under EUS guidance, followed by deployment of the proximal flange under endoscopic guidance. Once deployed, the gallbladder would be completely emptied by suction and irrigation until the effluent through the stent is clean.
BACKGROUND: Osteoporosis (OP) is a common systemic skeletal disorder characterized by an imbalance in bone homeostasis, involving increased osteoclastic bone formation and decreased osteoblastic bone resorption. Quercetin is a plant polyphenol that has been found to exhibit various biological activities, including antioxidant, anti-inflammatory, and antimicrobial effects. Previous studies have demonstrated its potential to improve postmenopausal OP, although the exact mechanism remains unclear. This study aims to investigate the anti-osteoporotic mechanism of quercetin based on the "intestinal flora - short-chain fatty acids (SCFAs) - inflammatory" signaling axis.
METHODS: In this study, we established an ovariectomized (OVX)-induced rat model, quercetin intervention and evaluated the effects on rats following antibiotic (ABX) treatment and fecal microbiota transplantation (FMT). After 6 weeks of intervention, the rats were euthanized, and samples from their femur, tibia, lumbar spine, serum, colon and feces were collected, and bone strength, intestinal flora structure, SCFAs levels and cytokine levels were assessed.
RESULTS: Quercetin modulates the intestinal flora by increasing potentially probiotic bacteria (i.e., Lactobacillales, Prevotellaceae, and Blautia) and decreasing potentially pathogenic bacteria (Desulfobacterota, Erysipelotrichales, Romboutsia, and Butyricoccaceae). It also increases SCFAs content and reduces colonic permeability by enhancing tight junction proteins (ZO-1, Occludin). Furthermore, quercetin lowers proinflammatory cytokine levels (LPS, IL-1β, and TNF-α), which enhances bone strength and prevents OVX-induced bone loss.
CONCLUSIONS: Quercetin may effectively reduce bone loss in OVX rats via the "intestinal flora - SCFAs - inflammatory" signaling pathway.
A modified HIF-1alpha protein is capable of avoiding degradation by failing to bind or failing to bind properly to pVHL. This modified HIF-1alpha protein can be used in research regarding hypoxic response as well as in treatments for hypoxicrelated conditions. The amino acids at residues (568, 570, 571, and 573) of HIF-1alpha play a critical role in mediating degradation at normoxia. Further, the pairs of residues at (560 and 561), and at (566 and 567) are critical as well. Resistance to pVHL-mediated degradation can be imparted using specific alterations at these residues. At least one amino acid residue selected from the group consisting of positions (390 through 531) may also be altered. Active fragments of the HIF-1alpha N-TAD may also be used as treatments for hypoxic-related conditions.
FIELD: chemistry. ^ SUBSTANCE: disclosed is a copolymer containing silicon groups, obtained via radical copolymerisation of at least one compound with radically polymerisable ,-ethylene-unsaturated double bond and at least one ionogenic and/or ionic group per molecule; and at least one radically polymerisable cross-linked compound, which contains at least two ,-ethylene-unsaturated double bonds per molecule in the presence of at least one organosilicon compound containing a polyether group and/or urethane(meth)acrylate, which contains siloxane groups. The copolymer has molar excess of anionogenic/anionic groups relative cationogenic/cationic groups. The invention also discloses a method of obtaining said copolymer and a cosmetic or pharmaceutical agent containing said copolymer. ^ EFFECT: powder can be easily added to thickening compositions and is suitable for modifying rheological characteristics of cosmetic and pharmaceutical compositions. ^ 18 cl, 2 tbl, 90 ex
Intracranial hypotension may result in pituitary gland enlargement due to compensatory hyperaemia and venous engorgement. Spontaneous intracranial hypotension (SIH) is frequently associated with connective tissue disorders predisposing patients to dural weakening including dural ectasia and meningeal diverticula. Symptoms of SIH typically include postural headache, dizziness and tinnitus. We present a case of a female in her 20s with Marfan syndrome and a history of pituitary adenoma, who reported intractable postural headaches. Hormonal workup revealed no abnormalities, whereas brain MRI showed sequelae of intracranial hypotension. Further MRI studies revealed thoracic and lumbar meningeal diverticula with significant dural sac ectasia at the L4-S2 level. Myelogram confirmed numerous lumbar spine diverticula with cerebrospinal fluid leak at the L5 and S1 right nerve roots. The patient underwent blood patch administrations at the level of the leak with improvement of symptoms.
Participants will receive potassium bicarbonate in dosage of 67.5 mmol/d. This compound has no other name.