Sensory stimuli can be easily realized with the active cooperation of the mother and, based on family-centered care, can help parents feel that they are playing an active role in their baby's care by being involved in their baby's care and can have positive effects on reducing the pain and stress of the baby during the heel blood collection process.As far as we know, there is no study in the literature investigating the effectiveness of multisensory stimulation applied by the mother during the heel blood collection procedure in reducing both the baby's pain and the mother's anxiety. It is thought that our study will provide new evidence to nursing care, that more than one sensory stimulus will be provided by the mother, will increase the baby's sense of confidence and reduce pain, contribute to the importance of family-centered care, and will be useful to support the results of the current study. This study aimed to examine the effect of multisensory stimulation applied by the mother during the heel blood collection procedure in newborns on reducing pain and to evaluate the effects of family-centered practice on the state anxiety of mothers.Before starting work, S.B.U. Ethical approval was obtained from the Süreyyapaşa Chest Diseases and Thoracic Surgery Clinical Research Ethics Committee. To start the research after the ethics committee's approval, T.C. Permissions were obtained from the Istanbul Provincial Health Directorate and the Istanbul Tuzla State Hospital management. In addition, written informed consent was obtained from the families of the newborns in the control and intervention groups. The research population consisted of all newborns born in Tuzla State Hospital in Turkey/Istanbul between July 2019 and January 2020 and whose infants were taken for routine metabolic screening by the Ministry of Health. The sample group consisted of 80 randomly assigned newborns, 40 in the intervention group and 40 in the control group. Randomization was provided according to the order of the procedure. The first newborn control group and the second newborn intervention group were formed. The newborns whose heel blood will be taken every day of the week during working hours continued from where they left off according to the order of the procedure. Before the procedure, the necessary materials were prepared, and the heel blood sheet information was filled in. The mothers of the babies whose heel blood was taken were informed about the heel blood screening and the research. The form containing the identification information of the baby and the mother was filled out. Before the procedure, the mother was asked to fill out the State Anxiety Scale (in both groups, the baby was with the mother). The newborns in the control group were taken to an open bed under a radiant heater, leaving the mother's side for routine heel blood collection applied in the hospital. First of all, the foot of the baby, who was taken to an open bed under a radiant heater, was warmed in the hands of the nurse for one minute, the area where the baby's heel blood would be taken was determined, the baby's heel was wiped with 70% alcohol with the help of a sponge and waited for it to dry, then heel lance was performed. The procedure was completed after the relevant sections on the pre-prepared heel blood sheet were filled with blood. A light touch was provided if necessary for the routine comfort of the baby for ethical reasons. The mothers of the babies whose heel blood was taken in the intervention group were informed about the steps of the procedure and the multisensory stimulation they would give to their babies (mother's voice, touching, breastfeeding, eye contact, mother's skin smell). The baby was started to be breastfed before the procedure and continued until the end of the evaluation period (1st stimulus). The mother supported her baby's back and bottom with one hand. He started to touch by positioning the thumb of the other hand to prevent the baby's nose from being blocked and the other fingers of the same hand to touch the baby's face (2nd stimulus). The mother started to talk with her baby in a gentle but firm tone, sometimes using sentences (words, lullabies, songs, etc.) that she wanted to increase the volume of her baby (3rd stimulus). Talking was started before the procedure and continued until the end of the evaluation period. The mother was in the baby's sight (4th stimulus). Clothes were removed as much as possible for the smell of the mother's skin (5th stimulus). After the baby's foot in the mother's lap was warmed in the nurse's hand for one minute, the area where the baby's heel blood would be taken was determined. After the baby's heel was wiped with 70% alcohol and a sponge and waited for it to dry, heel lance was performed. The procedure was completed after the relevant sections on the pre-prepared heel blood sheet were filled with blood. If the baby stopped sucking at any time during the procedure, the baby was placed back on the breast and the procedure continued from where it had left off. In the study, the data of the baby and the mother was prepared by the researcher mother's age, mother's occupation, gestational week of the baby, birth weight, gender, mode of delivery, etc.), the "Neonatal Infant Pain Scale (NIPS)" scale, which will be used in the assessment of the baby's pain, and the questionnaire form of the State Anxiety Scale to measure the mother's anxiety was used. NIPS score was evaluated by the researcher before the procedure, during the procedure, and 1 minute after the procedure. The State Anxiety Scale was administered to the mother before and after the procedure. The researcher calculated state anxiety scale scores.