anthonyyazdaniml/gliner-biomed-curated-corpus huggingface dataset:
<P>PROBLEM TO BE SOLVED: To provide a new delivery technique having the ability to transduce e.g. a nucleic acid into a cell and efficiently release a nucleic acid in a cell.  <P>SOLUTION: The new delivery technique comprises the following: A three-component conjugate of a nucleic acid such as antisense DNA or CpG DNA, a polysaccharide and carbon nanotube is formed. The resulting water-soluble conjugate is transduced into a cell and then irradiated with near-infrared rays to put the carbon nanotube to exothermic effect, thus enabling the nucleic acid to be released. The polysaccharide to be preferably used is a β-1,3-glucan(e.g. schizophyllan).  <P>COPYRIGHT: (C)2008,JPO&INPIT
BACKGROUND: The microbiota and metabolites in the gastrointestinal tracts of female animals at different reproductive periods are very important to the growth, development, and health of themselves and their offspring. However, the changes in the gastrointestinal microbiota and metabolites throughout reproductive period of different sheep breeds and their effects on the growth and development of offspring lambs are still unclear. Hence, this study presents an assessment of the reproductive hormone levels, immune levels, rumen microbiota, and metabolites in Hu sheep and Suffolk ewes at different reproductive periods and their effects on the growth and development of offspring lambs.
RESULTS: Hu sheep and Suffolk during non-pregnancy, pregnancy, and lactation were used as the research objects to determine reproductive and immune indexes of ewes at different periods, analyze rumen microbiome and metabolome, and track the growth performance and development of offspring lambs. The results showed that the reproductive hormone and immune levels of Hu sheep and Suffolk underwent adaptive changes across different reproductive periods. Compared with non-pregnancy, the microbial energy metabolism and lipid metabolism function decreased during Hu sheep pregnancy, and energy metabolism function decreased during lactation. In Suffolk, energy metabolism, glycan biosynthesis, and metabolism function were enhanced during pregnancy, and the metabolism of cofactors and vitamins was enhanced during lactation. Prevotella increased in Suffolk during pregnancy and lactation (P < 0.05) and was positively correlated with the birth weight and body size of the lambs (P < 0.05). Moreover, the abundances of Butyrivibrio and Rikenellaceae_RC9_gut_group during pregnancy were positively correlated with the intestinal immunity of the offspring lambs (P < 0.05), thereby regulating the intestinal immunity level of the lambs. Metabolomic analysis revealed that the protein digestion, absorption, and amino acid metabolism of Hu sheep were enhanced during pregnancy, which provided amino acids for the growth and development of pregnant ewes and fetuses and was significantly correlated with the birth weight, body size, and intestinal immunity of lambs (P < 0.05). Simultaneously, there was an increase in acetate and propionate during the pregnancy and lactation period of both Hu sheep and Suffolk, providing energy for ewes during reproductive period. Moreover, the microbiota during the lactation period was significantly correlated with the milk quality and lambs daily gain (P < 0.05).
CONCLUSIONS: This study revealed the characteristic succession changes in the rumen microbiota and its metabolites at different reproductive periods in sheep breeds and their regulation of reproductive hormone and immune levels and identified their potential effects on the growth and development of offspring lambs. The findings provide valuable insights into the health and feeding management of different sheep breeds during the reproductive stage. Video Abstract.
The effect of triiodothyronine (T3) on the phosphorylation of ERK and the occurrence and development of hepatocellular carcinoma (HCC) is controversial and remains to be clarified. In the present study, both in vitro (hepatoma cell lines) and in vivo (wild-type mice [WT] and mouse models of HCC [HrasG12Vand KrasG12Dtransgenic mice (Hras-Tg and Kras-Tg)]) systems were used to investigate the effect of T3 on p-ERK and hepatocarcinogenesis. The results showed that, in vitro, T3 treatment elevated the levels of p-ERK in hepatoma cells within 30 min. However, p-ERK levels returned to normal after 1 h with no significant effects on cellular proliferation or apoptosis. Interestingly, in vivo, T3 induced early rapid and transient activation of ERK and later persistent downregulation of p-ERK in liver tissues of WT. In Hras-Tg, liver weight, liver/body weight ratio, hepatic tumor numbers and sizes were significantly reduced withT3treatment compared with the untreated group. Furthermore, the levels of albumin, HrasG12V, and p-ERK in hepatic precancerous and tumor tissues were all significantly downregulated with T3 treatment; however, the levels of endogenous Hras were not affected. In WT, T3 also induced downregulation of Albumin in liver tissues, but without influence on the expression of endogenous Hras and p-MEK. Especially, the inhibitory effect of T3 on p-ERK and hepatic tumorigenesis and development without influence on the levels of KrasG12D and p-MEK was further confirmed in Kras-Tg. In conclusion, T3 suppresses hepatic tumorigenesis and development by independently and substantially inhibiting the phosphorylation of ERK in vivo.
BACKGROUND AND OBJECTIVES: Due to frailty, dermatosurgery in the elderly is preferably performed under tumescent local anesthesia, but data is limited. The aim was to evaluate tumescent local anesthesia for skin cancer surgery in the elderly with focus on clinical benefits (treatment processes, pain management) and local postoperative complication risk.
PATIENTS AND METHODS: Investigation of patients ≥ 75 years with inpatient head and neck skin cancer surgery under tumescent local anesthesia.
RESULTS: 2,940 procedures in 782 patients (mean age 83.3 years) were performed with the aim of complete tumor resection during the inpatient stay. 3.8 (range: 1-20) interventions were done over an average of 4.9 days (range: 1-28). 43.2% did not require any postoperative analgesia. 53.5% received NSAIDs, 3.3% opioids. Infection (13.6%) was the most common local postoperative complication. Surgical intervention due to bleeding was required in 2.8%. None was hemoglobin relevant or life-threatening. Suture dehiscence and necrosis were rare (0.6%).
CONCLUSIONS: Tumescent local anesthesia is an effective method for skin cancer surgery in the elderly. By avoiding general anesthesia, treatment processes can be optimized and anesthesiologic risks minimized. Local postoperative complications are still low and well treatable. The long-lasting analgesia results in a reduced need for analgesics and drug interactions.
PURPOSE:To obtain a new compound, having antagonistic actions on cholecystokinin-B/gastrin receptors and useful for preventing and treating gastric ulcer, duodenal ulcer, gastritis, regurgitant esophagitis, Zollinger-Ellison syndrome, etc. CONSTITUTION:This compound is expressed by formula I (R is isopropyl, isobutyl, benzyl or 4-hydroxyphenylmethyl), e.g. a compound expressed by formula II. Furthermore, the compound expressed by formula II (Q-20547-A) has the following physical properties: appearance: white solid; solubility: readily soluble in chloroform, methylene dichloride and acetone; soluble in methanol, ethanol and ethyl acetate and sparingly soluble in ether, n-hexane and water; color reaction: positive to iodine and the Ehrlich reaction: molecular formula: C40H36N6O4 and molecular weight: 664. Furthermore, the compound expressed by formula I is obtained by culturing a microorganism, belonging to the Aspergillus and having the ability to produce the compound expressed by formula I such as Aspergillus sp. Q20547 (FERM P-13662) in a culture medium.
All included patients will receive anesthesia consultation, biological assessment and chest CT scan in thin sections. A surgical treatment will always be planned after presentation of the file in a meeting of multidisciplinary consultation of thoracic oncology. The Archimedes® procedure will be performed during a bronchoscopy under general anesthesia. Immediate monitoring consisted in a chest x-ray 1hour after the procedure.
Patients after R0 surgery whose recurrence lesion larger than 5cm in diameter, or largest diameter was less than 5cm but with skip metastasis far from primary tumor or their time-to-recurrence longer than 16 months were assigned to involved field irradiation group. For lesions far from the thoracic stomach, the prescribed dose is 60Gy/2Gy/30f, and for lesions close to the thoracic stomach, the prescribed dose is 59.4-61.2Gy/1.8Gy/33-34f. Chest CT scan is planned at 50Gy. Radiation field should be modified according to the tumor response. Concurrent chemotherapy of paclitaxel and platinum was delivered every 3 weeks. PEG-rhG-CSF (3-6mg) should be given after 48 hours of chemotherapy.If patients received postoperative chemotherapy of paclitaxel and platinum and went through local-regional recurrence within six months, it is allowed to deliver chemotherapy regimens in the second line. Consolidate chemotherapy were adjusted to the patients after radiation therapy.
The invention provides a Paralichthys olivaceus streptococcus iniae GAPDH tandem multi-epitope polypeptide and application thereof, wherein the provided tandem multi-epitope polypeptide is four antigen epitope peptides connected by a polypeptide joint; wherein the amino acid sequences of the four antigen epitope peptides are respectively SEQ ID NO. 1-4. A specific amino acid sequence of the tandem polyepitope polypeptide is SEQ ID NO. 8. The invention also provides a streptococcus iniae GAPDH multi-epitope vaccine which is prepared by taking the tandem multi-epitope polypeptide as an antigen and a vaccine adjuvant. The prepared multi-epitope vaccine can provide stronger immune protection for the anti-S.initial infection of the paralichthys olivaceus, and the effect is obviously higher than that of a GAPDH recombinant protein subunit vaccine and an S.initial inactivated vaccine.
A biodegradable material for improving the regeneration of nerve cells is disclosed. The material includes a copolymer formed by reacting a first reactant selected from monomers, oligomers and polymers and a second charged reactant selected from charged monomers, charged oligomers and charged polymers. Nerve cells are contained within or attracted to the copolymer. The first reactant can be oligo(poly( ethylene glycol) fumarate). The charged reactant can be selected from unsaturated quaternary ammonium compounds. The material can include a photoinitiator such that the material is photocrosslinkable. The material can include a bioactive agent such as a nerve growth factor. In one form, the material is a hydrogel that can be injected as a fluid into a patient's body via minimally invasive arthroscopic techniques to form a scaffold for nerve tissue regeneration.
This paper aims to explore the anti-inflammatory mechanism of Saracae Cortex by using network pharmacology and molecular docking methods and verify it through the inflammation model of zebrafish. The effective components, potential core targets, and signaling pathways of Saracae Cortex were obtained by using network pharmacology. A lipopolysaccharide(LPS)-induced inflammation model of zebrafish was established to evaluate the anti-inflammatory activity of aqueous extract and 70% ethanol extract of Saracae Cortex with cell apoptosis rate and reactive oxygen species(ROS) production rate as indicators. q PCR was performed to verify the main targets predicted by network pharmacology. The prediction found that there were 121 potential anti-inflammatory targets in Saracae Cortex. Protein-protein interaction(PPI) analysis showed that Saracae Cortex mainly acted on signal transducer and activator of transcription 3(STAT3), vascular endothelial growth factor A( VEGFA), epidermal growth factor( EGF), tumor necrosis factor( TNF),tumor protein p53(TP53), matrix metalloprotein 9(MMP9), c-fos proto-oncogene protein(FOS), estrogen receptor 1(ESR1), cx-c motif chemokine ligand 8(CXCL8), cluster of differentiation 8(CD8), and other targets. Gene Ontology(GO) analysis showed the biological process mainly acted on the inhibition of apoptosis, the positive regulation of gene expression, and the positive regulation of cell proliferation. Kyoto Encyclopedia of Genes and Genomes(KEGG) analysis showed that the mitogen-activated protein kinase(MAPK) signaling pathway, PI3K-Akt signaling pathway, and hypoxia-inducible factor 1(HIF-1) signaling pathway may play a key role in anti-inflammation of Saracae Cortex. Molecular docking verified that five key compounds had a strong binding force with their corresponding core target. Zebrafish animal experiments showed that Saracae Cortex could significantly inhibit ROS formation and reduce cell apoptosis in juvenile fish caused by inflammation and inhibit the further enhancement of inflammatory response in tissue. In addition, compared with the blank group, the transcription levels of nuclear factor kappa-B(NF-κB), TP53, FOS, adaptor protein complex-1(AP-1), and mitogen-activated protein kinases P38(P38) were significantly up-regulated in the model group. Compared with the model group, the m RNA expression of NF-κB, TP53, FOS, AP-1, and P38 was significantly down-regulated in zebrafish tissue treated with aqueous extract and 70% ethanol extract of Saracae Cortex. Saracae Cortex plays an anti-inflammatory role through multiple components and targets, and its anti-inflammatory effect may be related to the inhibition of the MAPK signaling pathway.
PURPOSE:To jet oxidation potential water into a uterus for cleaning to eradicate pyogenic bacteria, keep the inside of the uterus clean and increase the implanta tion rate of blastocyst. CONSTITUTION:City water is combined with a prescribed amount of electrolytes from the feeder 3, then goes in the electrolytic bath 4. The bath 4 is divided with an ion-permeating diaphragm 41 into the cathode cell 42 into which the cathode is inserted and the anode cell 43 into which an anode is inserted and direct current voltage is applied between the cathode and the anode. The oxidation potential water outlet from the electric bath is measured on its ORP (oxidation and reduction potential) and pH and passed through the reverse flow-stopping valve 11 to the tank 6, if these values are in prescribed ranges. Other water having values out of the range is expelled through the three-way valve 12. The oxidation potential water usually has about 1,000-1,090mV ORP and less than 2.8pH and functions as a cleaning solution for the inside of uterus. The cleaning water in the tank 6 is pressurized directly or after tentative storage in a dark bottle, then injected into the inside of uterus.
BACKGROUND: As one of the most common malignancies, cervical cancer (CC) seriously affects women's health. This study aimed to investigate the biological function of Serinc2 in CC.
METHODS: Serinc2 expression was surveyed utilizing immunohistochemistry, western blot, and qRT-PCR. CC cell viability, invasion, proliferation, migration, and apoptosis, were detected via CCK-8, Transwell assay, colony formation, wound healing assay, and flow cytometry. Glucose consumption, lactate production, and ATP levels were determined by the corresponding kit. The protein expression of c-Myc, PDK1, HK2, PFKP, LDHA, Snail, Vimentin, N-cadherin, and E-cadherin was detected via western blot. The interaction between the promoter of PFKP and Myc was confirmed through luciferase reporter assay and Chip assay. In vivo, to evaluate the function of Serinc2 on tumor growth, a xenograft mouse model was used.
RESULTS: In CC tissues and cells, Serinc2 was upregulated. In CC cells, knockdown of Serinc2 suppressed cell invasion, proliferation, migration, decreased the expression of Snail, Vimentin, N-cadherin, HK2, PFKP, LDHA, and PDK1, increased E-cadherin expression, reduced glucose consumption and the production of lactate and ATP, and induced cell apoptosis; Serinc2 overexpression led to the opposite results. Mechanically, Serinc2 promoted Myc expression, and Myc induced PFKP expression. Furthermore, overexpressed Myc abolished the inhibitive influences of Serinc2 knockdown on the malignant behaviors of CC cells. Additionally, knockdown of Serinc2 inhibited tumor growth and reduced the protein expression of c-Myc, PFKP, LDHA, and PDK1 in vivo.
CONCLUSIONS: Knockdown of Serinc2 inhibited the malignant progression of CC, which was achieved via Myc pathway. Our study provides novel insight into CC pathogenesis.
Patients receive binimetinib PO BID on days 1-28 and palbociclib PO QD on days 1-21. Treatment repeats every 28 days for up to 24 cycles in the absence of disease progression or unacceptable toxicity.
OBJECTIVE: Exploring the perinatal outcomes of forceps delivery and the risk factors of postpartum hemorrhage, laying a certain foundation for early identification of indications for forceps assisted delivery and suppressing the risk of bleeding during forceps assisted delivery, and improving delivery quality.
METHOD: Retrospective analysis was made on the clinical information of 1520 parturients delivered by vagina in hospitals from December 2019 to December 2021. They were divided into normal vaginal delivery group (sample size=1454) and forceps assisted delivery group (sample size 66) according to whether forceps-assisted delivery occurred during the second stage of labor. They were divided into a postpartum hemorrhage group (sample size 9) and non-postpartum hemorrhage group (sample size 47) according to whether forceps-assisted delivery occurred, the risk factors of postpartum hemorrhage were analyzed by logistic regression.
RESULT: The incidence of perinatal infants in the forceps assisted delivery group compared to those in the normal vaginal delivery group who were transferred to the neonatal intensive care unit (25.76% vs 9.97%), neonatal asphyxia (4.55% vs 1.03%), shoulder dystocia (1.52% vs 0.69%), and facial scratches (40.91% vs 0.14%) was statistically significant (P < .05), except for shoulder dystocia. Univariate analysis showed that abnormal coagulation function, fetal orientation during midwifery, soft birth canal laceration, perineum lateral incision, and neonatal birth weight were the single factors related to postpartum hemorrhage during forceps delivery (P < .05). Multivariate analysis showed that abnormal coagulation function, laceration of the soft birth canal, and lateral episiotomy were independent risk factors for postpartum hemorrhage during forceps-assisted delivery. The rate of postpartum hemorrhage under forceps-assisted delivery was relatively low when the fetal orientation was occipital transverse (P < .05).
CONCLUSION: The incidence of postpartum hemorrhage in the forceps assisted delivery group is higher, with occipital posterior position, abnormal coagulation function, soft birth canal tear, and lateral perineal incision being high-risk factors for postpartum hemorrhage in forceps assisted delivery. We need to strengthen prevention and control measures to improve the quality of the perinatal period. This study has guiding significance for early identification of high-risk factors for postpartum hemorrhage, strengthening pre pregnancy knowledge education, strengthening labor process monitoring, actively correcting fetal orientation, and improving midwifery techniques.
Belonging to the field of cosmetics, the invention in particular relates to a henna natural hair dye. According to the invention, the red pigment of henna is employed as the raw material to prepare the henna natural hair dye. The specific making process includes: air drying picked fresh henna naturally, then grinding the henna into powder, adding an appropriate amount of alum and performing mixing, thus obtaining the henna natural hair dye. Henna pigment and metal ions can react to produce new color developing molecules, and the products can also be made into new hair dyes. Henna red pigment and iron ions react to generate a black dye, which can be made into a black hair dye. To shorten the hair dying time, softening treatment is carried out on hair during hair dying. A hair softening agent is prepared. During storage, the hair softening agent and the dye agent are separately packaged and stored, and during hair dying, the two are mixed together for use.
Spinal anesthesia is employed to provide excellent surgical conditions in case of C-Section. It is the anesthetic technique of choice among obstetric patients. However it is associated with sympathectomy which reduces the venous return and ultimately causes hypotension. This hypotension is mainly due to redistribution of blood volume to the peripheral compartment. Perfusion index which is measured by Pulse Oximetery is a ratio of pulsatile to non-pulsatile blood in the peripheral compartment of the body. As spinal anesthesia causes sympathectomy, peripheral blood volume increases. As a result perfusion index value should rise. This will then co-relate with hypotension. In simple terms those individuals who have a high baseline Perfusion index value will more likely to develop hypotension. This study aims to identify the baseline perfusion index value which co-relates with development of hypotension following sub-arachnoid block for C-section.
The present invention relates to a technique in which an EDC system collects clinical trial data included in electronic healthcare records from an EHR system by using a method for converting electronic healthcare records into clinical trial data, the technique in which the EDC system requests, to the EHR system, the clinical trial data included in the electronic healthcare record, which the EHR system has stored, with queries, receives a response to the request, and stores the clinical trial data in the EDC system without re-input of users, unlike the conventional method in which users of an EDC system directly input even clinical trial data included in electronic healthcare records on an electronic recording paper provided by an EDC system. The EDC and EHR systems of the present invention transmit and receive queries and responses using international standard XML messages so as to request data even when the EDC and EHR systems do not identify the internal data structure of each other. More specifically, the EDC system accesses the EHR system of hospitals which participate in clinical trials, requests, using a QED request message, clinical trial data included in electronic healthcare records having a CCD format to the EHR system, receives, using a QED response message, the result of a searched CCD form, converts the result into an SDTM data set, and stores the SDTM data set in the internal database system of the EDC system. [Reference numerals] (702) Have an EDC system to make an inquiry request;(704) QED request XML form ?;(706) Convert the inquiry into a QED request XML form;(708) Transmit the QED request XML inquiry to an EHR system;(710) Convert the QED request XML inquiry into an EHR DBS inquiry;(712) Have the EHR DBS to precess the converted inquiry and generate the results;(714) CCD XML form ?;(716) Convert the inquiry results into a CCD XML form;(718) Transmit the CCD XML inquiry results to an EDC system;(720) User checked ?;(722) Store the inquiry results in the EDC DBS;(724) Next inquiry ?
The target INR is 2.5. Warfarin initiation is via Pharmacogenetic dosing.