Dental implantation is the most widely used method for treating edentulous patients. The most important factors affecting dental implant success are the patient's systemic condition, the material used, the physical properties of the implant, and the surgical protocol. Flap design is the first and most important stage of dental implant surgery. The most commonly used surgical technique for implant placement elevates the full-thickness mucoperiosteal flap. Flap design is critical in this procedure to reduce patient pain, limit stress-related cortisol level increases, and prevent peri-implant bone loss. An ideal flap reveals the entire surgical area, allowing the surgeon to work comfortably and place the implant in the desired position; thus, a vertical incision can be added to the flap. However, recent studies have demonstrated that a minimally invasive flap design reduces postoperative pain, bleeding, crestal bone loss, and surgical time. Several other studies concluded that flap designs with or without a vertical incision are equally successful.

Marginal bone resorption also influences long-term implantation success. Marginal bone resorption is aggravated by flap design and increases in cortisol secretion due to patient pain. High cortisol and β-endorphin concentrations can significantly increase MMP-1, MMP-2, MMP-7, MMP-11, and TIMP-1 levels in human gingival fibroblasts, explaining the increase in periodontal and peri-implant destruction associated with physiological stress. Several studies have offered acceptable explanations for the relationship between oral health (especially periodontal health) and psychological stres. The pathophysiological effects of periodontal disease are directly related to host resistance and affect the immune system. A positive relationship between salivary cortisol levels and stress levels has also been observed in individuals with severe periodontal disease. Marginal bone resorption around implants can be measured biochemically by examining substances in the peri-implant crevicular fluid, such as the markers osteoprotegerin (OPG) and receptor-activator NFkB ligand (RANKL). RANKL is a membrane-bound factor expressed on the outer surfaces of osteoblast cells and plays a central role in osteoclast cell formation and development. OPG binds to RANKL and prevents it from interacting with its receptor, NFkB, on osteoclasts and preosteoclasts, preventing bone loss. The OPG/RANKL ratio in the peri-implant groove fluid can indicate marginal bone resorption around dental implants; healthy implants demonstrate greater OPG/RANKL ratios than those with active marginal bone resorption. Thus, the effects of flap design on marginal bone loss (MBL) can be compared via the OPG/RANKL ratio.

Pain also causes systemic changes. The levels of cortisol, dehydroepiandrosterone, testosterone, and thyroid-stimulating hormone in bodily fluids can indicate a patient's pain level. Cortisol is controlled by adrenocorticotropic hormone (ACTH) released from the anterior pituitary gland. Most physical or neurological stressors strongly increase ACTH secretion, and dental stress increases bodily fluid hormone levels.

A literature review revealed insufficient studies examining the effects of a vertical incision on the peri-implant bone level. Existing studies only include clinical or radiological evaluations. This study aimed to evaluate the clinical effects of flap design and incision on dental implants by administering a visual analog scale (VAS) questionnaire, measuring swelling, recording the flap surgery duration, assessing the OPG/RANKL ratio and cortisol levels in the peri-implant groove fluid, and taking periapical X-ray images. Our study is unique and comprehensive since it addresses the clinical, biochemical, and radiological effects of a vertical incision on implants. In addition, although various studies have included cortisol measurements in the gingival crevicular fluid, few have measured cortisol in peri-implant groove fluid. Our findings suggest that flap designs with a vertical incision cause early marginal bone loss and additional stress on dental implant recipients.