anthonyyazdaniml/gliner-biomed-curated-corpus huggingface dataset:
The blood supply in the retina ensures photoreceptor function and maintains regular vision. Leber's hereditary optic neuropathy (LHON), caused by the mitochondrial DNA mutations that deteriorate complex I activity, is characterized by progressive vision loss. Although some reports indicated retinal vasculature abnormalities as one of the comorbidities in LHON, the paracrine influence of LHON-affected retinal ganglion cells (RGCs) on vascular endothelial cell physiology remains unclear. To address this, we established an in vitro model of mitochondrial complex I deficiency using induced pluripotent stem cell-derived RGCs (iPSC-RGCs) treated with a mitochondrial complex I inhibitor rotenone (Rot) to recapitulate LHON pathologies. The secretomes from Rot-treated iPSC-RGCs (Rot-iPSC-RGCs) were collected, and their treatment effect on human umbilical vein endothelial cells (HUVECs) was studied. Rot induced LHON-like characteristics in iPSC-RGCs, including decreased mitochondrial complex I activity and membrane potential, and increased mitochondrial reactive oxygen species (ROS) and apoptosis, leading to mitochondrial dysfunction. When HUVECs were exposed to conditioned media (CM) from Rot-iPSC-RGCs, the angiogenesis of HUVECs was suppressed compared to those treated with CM from control iPSC-RGCs (Ctrl-iPSC-RGCs). Angiogenesis-related proteins were altered in the secretomes from Rot-iPSC-RGC-derived CM, particularly angiopoietin, MMP-9, uPA, collagen XVIII, and VEGF were reduced. Notably, GeneMANIA analysis indicated that VEGFA emerged as the pivotal angiogenesis-related protein among the identified proteins secreted by health iPSC-RGCs but reduced in the secretomes from Rot-iPSC-RGCs. Quantitative real-time PCR and western blots confirmed the reduction of VEGFA at both transcription and translation levels, respectively. Our study reveals that Rot-iPSC-RGCs establish a microenvironment to diminish the angiogenic potential of vascular cells nearby, shedding light on the paracrine regulation of LHON-affected RGCs on retinal vasculature.
Targeted biopsies will be performed by software-assisted MRI-ultrasound fusion registration . Software-based fusion targeted biopsy of 4 cores per target followed by 12-core systematic biopsy will be performed. The fusion or overlay of 3D MRI and USG images create a detailed 3D prostate image with both targeted and systematic biopsy core locations recorded.
Delirium is a common yet under diagnosed condition in cardiac surgery patients, and may cause prolonged cognitive impairment and increased risk of complications. Patients are at risk of e.g. pulling catheters and lines and may fall attempting to get out of bed. The aging patient population present with many risk factors for developing delirium, but diagnostic tools have been few. Almost 50% present with hypoactive delirium, which is often not diagnosed, nor treated correctly. To optimize effect, treatment should be initiated early, maintained until clinical improvement is observed, and then tapered gradually. Recently, Delirium Observation Screening scale (DOS scale) was developed and validated in elective cardiac surgery patients in the Netherlands, with interesting results. This encouraged us to evaluate the effects of systematic delirium screening and treatment in cardiac surgery patients. To our knowledge, no prior studies have evaluated use of DOS scale in this context.

OBJECTIVES To evaluate the incidence and severity of delirium, and the effects of standardized treatment in a population of Danish cardiac surgery patients.
The world continues to contend with COVID-19, fueled by the emergence of viral variants. At the same time, a subset of convalescent individuals continues to experience persistent and prolonged sequelae, known as long COVID. Clinical, autopsy, animal and in vitro studies all reveal endothelial injury in acute COVID-19 and convalescent patients. Endothelial dysfunction is now recognized as a central factor in COVID-19 progression and long COVID development. Different organs contain different types of endothelia, each with specific features, forming different endothelial barriers and executing different physiological functions. Endothelial injury results in contraction of cell margins (increased permeability), shedding of glycocalyx, extension of phosphatidylserine-rich filopods, and barrier damage. During acute SARS-CoV-2 infection, damaged endothelial cells promote diffuse microthrombi and destroy the endothelial (including blood-air, blood-brain, glomerular filtration and intestinal-blood) barriers, leading to multiple organ dysfunction. During the convalescence period, a subset of patients is unable to fully recover due to persistent endothelial dysfunction, contributing to long COVID. There is still an important knowledge gap between endothelial barrier damage in different organs and COVID-19 sequelae. In this article, we mainly focus on these endothelial barriers and their contribution to long COVID.
NMES+FES group (n=24; 2 days/week for 24 weeks); this group will undergo twice weekly of 12 weeks of surface NMES and ankle weights followed by 12 additional weeks of twice weekly of progressive FES-LEC using the RT300 bike. The total participation duration is 24 weeks +3 weeks for measurements.
NEW MATERIAL:A compound shown by the formula I (R<1> and R<2> are H, or lower alkyl). USE:Having fragrance like musk, useful as a perfume component, or an intermediate for synthesizing muscone, exalton, etc. expensive natural perfume. PROCESS:Cyclododecanone is reacted with a lithium dialkyl amide to form an anion, which is reacted with an allyl halide shown by the formula II (X<1> is halogen) at -80-50 deg.C to give a compound shown by the formula III, which is reacted with hydroxyamine by conventional procedure under dehydrating conditions by heating to give an oxide shown by the formula IV. This compound is then sulfonylated with methanesulfonyl chloride, etc. to give a sulfonyl compound, which is subjected to Beckmann rearrangement in the presence of a catalyst such as trialkylsilyl triflate to give a ketimine shown by the formula V, which is hydrogenated, to give a compound shown by the formula I a.
The invention discloses a method for drying gastrodia elata bl. at a variable temperature, belonging to the technical field of preliminary processing of medicinal plants. The method mainly comprises the following steps of: steaming the fresh gastrodia elata bl., reducing the temperature of the steamed gastrodia elata bl. to room temperature, dehydrating the steamed gastrodia elata bl., and drying the gastrodia elata bl at the variable temperature. Compared with the traditional method, because the gastrodia elata bl. is graded by weight and steamed separately, the problem that the gastrodia elata bl. which differs greatly in weight can not be steamed thoroughly at the same time can be solved, and the insufficient or overmuch steaming of the gastrodia elata bl. can be avoided. Because the drying temperature of the gastrodia elata bl. is increased gradually, the rapid loss of water of the gastrodia elata bl. can be prevented, and the shrinkage of the peel of the gastrodia elata bl. can be reduced. Compared with the traditional method, the method disclosed by the invention has the following advantages: under the condition that the outer quality and the inner quality of the gastrodia elata bl. are guaranteed, the drying time of the gastrodia elata bl. can be shortened by 10-15 days, particularly, no chemical agent (especially sulphur) can be used, or the pollution from sulphur in firing coal can be avoided, and the content of gastrodin in the gastrodia elata bl. can be increased by more than 5%.
BACKGROUND: One of the most severe complications of primary total knee arthroplasty (TKA) is prosthetic joint infection. Currently, the use of antibiotic-loaded cement for the prevention of infection is still controversial. The aim of the present study was to evaluate if the use of antibiotic-loaded cement reduces the infection rate in primary TKA in long-term follow-up (more than 5 years average follow-up).
METHODS: This study is the follow-up extension of a prospective randomized study, with 2,893 cemented TKA performed between 2005 and 2010 at our institution. There were 2 different cohorts depending on which bone cement was used: without antibiotics (control group) or those loaded with erythromycin and colistin (study group). All patients received the same systemic prophylactic antibiotics. The patients were followed for a minimum of twelve months. The diagnosis of prosthetic joint infection was done according to Zimmerli criteria.
RESULTS: In 1,452 patients, the prosthetic components were fixed using bone cement without antibiotics, whereas in 1,441 patients, bone cement was loaded with erythromycin and colistin. Both groups were comparable in terms of all the possible risk factors studied. We found a total of 53 deep infections, with a mean rate of 1.8%. There were no differences between the groups as to whether bone cement with or without antibiotics had been used (P = .58). The average duration of follow-up was 8.7 years. In terms of prosthetic revision due to aseptic loosening, there were no differences between groups (P = .32), with 33 revision arthroplasties in the control group and 37 in the study group. Moreover, we analyzed the erythromycin resistance rate, with no differences between both groups (P = .6).
CONCLUSIONS: The use of erythromycin and colistin-loaded bone cement in TKA did not lead to a decrease in the rate of infection in long-term follow-up, a finding that suggests that its use would not be indicated in the general population.
The invention provides a medicinal pillow for preventing paediatric otitis media and a preparation method thereof. The medicinal pillow consists of a pillow cover, a pillow interior and a Chinese medicine bag, wherein the pillow cover has a low-middle high-exterior structure made of full cotton. When a child sleeps, the head rests at a slightly higher position so as not to make mucus accumulated in a nasal cavity flow into an auditory tube; therefore, swelling and infarction of the nasal cavity is relieved so as to avoid the otitis media. Traditional Chinese medicines in the Chinese medicine bag are bupleurum, cape jasmine fruit, baical skullcap root, oriental waterplantain rhizome, plantain herb, akebia stem, dandelion, immature bitter orange and licorice root; and the medicinal pillow is prepared by the following steps of: mixing, crushing and grinding the medicines into fine powder; packing the powder by using gauze and putting into the pillow interior; and loading the pillow interior into the pillow cover to obtain the medicinal pillow for preventing the paediatric otitis media. Due to mutual conductance between a human body and the traditional Chinese medicines during sleep, the medicines enter the nasal meatus to achieve the effects of clearing heat, removing toxin, removing dampness and diminishing swelling; therefore, the aim that the medicinal pillow and the traditional Chinese medicines prevent the paediatric otitis media synchronously is fulfilled. The Chinese medicine bag in the medicinal pillow is replaceable, and is convenient, rapid and sanitary to use.
The present invention discloses oral liquid for treating hemorrhoids, which is prepared from sophora flower, garden burnet root, scolopendra, peach kernel, Chinese pulsatilla root, rhubarb, corydalis tuber and Chinese arborvitae twig as raw materials by processes of drying and grinding operation, decoction, concentration, etc. The present invention has the efficiency of quickly relieving inflammation, alleviating pain, softening and resolving hard mass, activating blood circulation to dissipate blood stasis, eliminating necrotic tissue and promoting granulation. The present invention has unique curative effects on internal hemorrhoids, external hemorrhoids, mixed hemorrhoids, thrombosed hemorrhoids, pink hemorrhoids, anus fissure, hemafecia and anal and intestinal diseases. The diseases can be once cured without operations. The oral liquid takes effect after two days of administration by patients; the diseases can be healed within seven days and can be radically healed within ten days. Tested by 100 clinical cases, the total effective rate is higher than 98%.
Individuals receive 15 sessions of ADAPT-locomotor training for 3 weeks. During ADAPT-locomotor training, stepping in response to obstacles and walking challenges are practiced on a treadmill and overground.
Acute spinal cord injury (SCI) always results in sustainable recruitment of inflammatory cells driven by sequentially generated chemokines, thereby eliciting excessive neuroinflammation. However, the underlying mechanism of temporally produced chemokines remains elusive. Reactive astrocytes are known to be the main sources of chemokines at the lesion site, which can be immediately activated by thrombin following SCI. In the present study, SCI was shown to induce a sequential production of chemokines CCL2 and CCL5 from astrocytes, which were associated with a persistent infiltration of macrophages/microglia. The rapidly induced CCL2 and later induced CCL5 from astrocytes were regulated by thrombin at the damaged tissues. Investigation of the regulatory mechanism revealed that thrombin facilitated astrocytic CCL2 production through activation of ERK/JNK/NFκB pathway, whereas promoted CCL5 production through PLCβ3/NFκB and ERK/JNK/NFκB signal pathway. Inhibition of thrombin activity significantly decreased production of astrocytic CCL2 and CCL5, and reduced the accumulation of macrophages/microglia at the lesion site. Accordingly, the locomotor function of rats was remarkably improved. The present study has provided a new regulatory mechanism on thrombin-mediated sequential production of astrocytic chemokines, which might be beneficial for clinical therapy of CNS neuroinflammation.
The most general cancer in men is prostate cancer (PCa), with its risk increasing due to age and obesity. Visfatin, a member of adipokines, is related to cancer progression and metastasis, but its relationship in PCa remains undetermined. In addition, no knowledge is available regarding relations between visfatin polymorphisms and clinicopathological characteristics in PCa. We sought to investigate the functions of four visfatin gene polymorphisms and clinicopathological characteristics on the hazard of developing PCa in 695 Taiwanese males with PCa. Carriers of the GA+AA heterozygote of SNP rs61330082 were at a markedly higher risk of biochemical recurrence than those with the GG genotype. Visfatin rs61330082 and rs11977021 were related with a high risk of perineural invasion, lymphovascular invasion, and biochemical recurrence in prostate-specific antigen (PSA) > 10 PCa patients. The Cancer Genome Atlas database noted that visfatin mRNA level did not prominently differ with pathological T/N stage and overall survival. This finding is the first to document a connection between visfatin polymorphisms and clinicopathological characteristics of PCa in Taiwanese males.
Obesity is associated with alterations in lipid metabolism and gut microbiota dysbiosis. This study investigated the effects of puerarin, a bioactive isoflavone, on lipid metabolism disorders and gut microbiota in high-fat diet (HFD)-induced obese mice. Supplementation with puerarin reduced plasma alanine aminotransferase, liver triglyceride, liver free fatty acid (FFA), and improved gut microbiota dysbiosis in obese mice. Puerarin's beneficial metabolic effects were attenuated when farnesoid X receptor (FXR) was antagonized, suggesting FXR-mediated mechanisms. In hepatocytes, puerarin ameliorated high FFA-induced sterol regulatory element-binding protein (SREBP) 1 signaling, inflammation, and mitochondrial dysfunction in an FXR-dependent manner. In obese mice, puerarin reduced liver damage, regulated hepatic lipogenesis, decreased inflammation, improved mitochondrial function, and modulated mitophagy and ubiquitin-proteasome pathways, but was less effective in FXR knockout mice. Puerarin upregulated hepatic expression of FXR, bile salt export pump (BSEP), and downregulated cytochrome P450 7A1 (CYP7A1) and sodium taurocholate transporter (NTCP), indicating modulation of bile acid synthesis and transport. Puerarin also restored gut microbial diversity, the Firmicutes/Bacteroidetes ratio, and the abundance of Clostridium celatum and Akkermansia muciniphila. This study demonstrates that puerarin effectively ameliorates metabolic disturbances and gut microbiota dysbiosis in obese mice, predominantly through FXR-dependent pathways. These findings underscore puerarin's potential as a therapeutic agent for managing obesity and enhancing gut health, highlighting its dual role in improving metabolic functions and modulating microbial communities.
BACKGROUND: Assessing the umbilical artery pulsatility index via Doppler measurements plays a crucial role in evaluating fetal growth impairment.
OBJECTIVE: This study aimed to investigate perinatal outcomes associated with discordant pulsatility indices of umbilical arteries in fetuses with growth restriction.
STUDY DESIGN: In this retrospective cohort study, all singleton pregnancies were included if their estimated fetal weight and/or abdominal circumference fell below the 10th percentile for gestational age (2017-2022). Eligible cases included singleton pregnancies with concurrent sampling of both umbilical arteries within 14 days of birth at the ultrasound evaluation closest to delivery. The exclusion criteria included births before 22 weeks of gestation, evidence of absent or reverse end-diastolic flow in either umbilical artery, and known fetal genetic or structural anomalies. The study compared cases with discordant umbilical artery pulsatility index values (defined as 1 umbilical artery pulsatility index at ≤95th percentile and the other umbilical artery pulsatility index at >95th percentile for gestational age) to pregnancies where both umbilical artery pulsatility indices had normal pulsatility index values and those with both umbilical arteries displaying abnormal pulsatility index values. The primary outcome assessed was the occurrence of composite adverse neonatal outcomes. Multivariable logistic regressions were performed, adjusting for relevant covariates.
RESULTS: The study encompassed 1014 patients, including 194 patients (19.1%) with discordant umbilical artery pulsatility index values among those who had both umbilical arteries sampled close to delivery, 671 patients (66.2%) with both umbilical arteries having normal pulsatility index values, and 149 patients (14.7%) with both umbilical arteries exhibiting abnormal values. Pregnancies with discordant umbilical artery pulsatility index values displayed compromised sonographic parameters compared with those with both umbilical arteries showing normal pulsatility index values. Similarly, the number of abnormal umbilical artery pulsatility index values was associated with adverse perinatal outcomes in a dose-response manner. Cases with 1 abnormal (discordant) umbilical artery pulsatility index value showed favorable sonographic parameters and perinatal outcomes compared with cases with both abnormal umbilical artery pulsatility index values, and cases with both abnormal umbilical artery pulsatility index values showed worse sonographic parameters and perinatal outcomes compared with cases with discordant UA PI values. Multivariate analysis revealed that discordant umbilical artery pulsatility indices were significantly and independently associated with composite adverse perinatal outcomes, with an adjusted odds ratio of 1.75 (95% confidence interval, 1.24-2.47; P = .002).
CONCLUSION: Evaluating the resistance indices of both umbilical arteries may provide useful data and assist in assessing adverse perinatal outcomes among fetuses with growth restriction.
PURPOSE:To obtain the titled compound having improved stability to heat and light and excellent storage stability and useful as pharmaceuticals, foods, etc., by clathrating a liquid oil with cyclodextrin or methylated cyclodextrin. CONSTITUTION:A liquid oil selected from a medium-chain fatty acid triglyceride and an ester-exchanged oil of an edible oil and a medium-chain fatty acid triglyceride is clathrated with cyclodextrin or a methylated cyclodextrin of formula (A is methyl or H; n is 6-9; at least one of 3n A groups is methyl). The production of the clathrate compound is carried out by conventional process such as saturated ethanol solution process, kneading process, etc. The amount of the cyclodextrin is preferably 1-10pts.wt. per 1pt.wt. of the medium-chain fatty acid triglyceride or the ester-exchanged oil of an edible oil and a medium- chain fatty acid triglyceride.
The invention relates to the technical field of processing of traditional Chinese medicine materials, in particular to equipment and a method for sulphur fumigation of traditional Chinese medicine materials and medicinal slices. The method is characterized by comprising the following steps: 1) placing the traditional Chinese medicine materials, i.e. uniformly paving the traditional Chinese medicine materials to be subjected to fumigation in a sulphur fumigation box; 2) adding sulphur proportionally according to the amount of the added traditional Chinese medicine materials; 3) burning the sulphur, setting temperature, time and air pressure in the fumigation box, and subjecting the traditional Chinese medicine materials to fumigation by adopting vacuum and low temperature; 4) monitoring and adjusting temperature and concentration of sulfur dioxide as well as the air pressure in real time; and 5) absorbing and processing sulfur dioxide waste gas. According to the method and equipment for sulphur fumigation of the traditional Chinese medicine materials, controllable operation of the sulphur fumigation process of the traditional Chinese medicine materials is realized. Through controlling key factors such as temperature, concentration of oxygen and sulfur dioxide and the like in the fumigation process, the loss of active ingredients in the traditional Chinese medicine materials is reduced, and the residual quantity of sulfur dioxide and heavy metal in the traditional Chinese medicine materials can be controlled, therefore, the quality of the traditional Chinese medicine materials can be ensured to the maximum degree. The equipment has the characteristics of convenience in operation, controllable fumigation parameters, low cost, convenience in generalization and the like.