anthonyyazdaniml/gliner-biomed-curated-corpus huggingface dataset:
PURPOSE:The titled antibody produced from a hybridoma formed by the cell fusion of a cell from a tumorous line of a mouse and a splenic cell of a mouse immunized with cortisol haptene antigen. CONSTITUTION:A monoclonal anticortisol antibody prepared by fusing a cell, e.g. P3-X63-8AZ, from a tumorous line of a mouse to a splenic cell from a mouse immunized with a cortisol haptene antigen, etc. dissolved in an isotonic buffer solution or physiological saline solution, etc., administered to one mouse in 10-300mum dose. The resultant monoclonal anticortisol antibody is obtained from the formed hybridoma. The cortisol in a sample can be determined by subjecting a cortisol labeled with an enzyme and the cortisol in a sample to the competitive reaction, and measuring the amount of the enzyme linked to the antibody. EFFECT:The aimed antibody of always stabilized quality is obtained by preparing the antibody from the resultant produced hybridoma without preparing an antigen nor the influence of the individual difference of an animal.
The present invention provides novel genes coding for antiviral  proteins  isolated  from genomic  DNA and genomic library of Phytolacca insularis Nakai, and antiviral proteins having the amino acid sequences deduced from the said genes, expression vectors containing the said genes, and processes for preparing   the   antiviral   proteins   from   recombinant microorganisms transformed with the said vectors.  gPIP2 gene of the present invention coding for a mature protein consists of 882bp including initiation codon of translation, and its open reading frame encodes a 32.8kDa-protein which consists of 292 amino acids including initiation methionine.  gPIP50 gene of the invention coding for a mature protein consists of 951bp including initiation codon of translation, and its open reading frame encodes a 35.7kDa-protein which consists of 315 amino acids  including  initiation methionine.   gPIP50  and gPIP2 proteins were successfully produced in a form of inclusion body in the microorganisms transformed with vectors containing the said gPIP2 and gPIP50 genes.   Accordingly, PIP2 and gPIP50 genes isolated from Phytolacca insularis Nakai, can be employed in the manufacture  of transgenic plants having antiviral resistance.  Also, the recombinant gPIP2 and gPIP50 proteins prepared  from  the  transformed  microorganisms  harboring expression vectors containing gPIP2 and gPIP50 genes can be used as an active ingredient of antiviral agents of plant viruses, and employed in the manufacture of immunoconjugate for the treatment of AIDS and cancer.
Based on the mechanism of action, SARCLISA can cause fetal harm when administered to a pregnant woman. SARCLISA may cause fetal immune cell depletion and decreased bone density. Advise pregnant women of the potential risk to a fetus. Advise females with reproductive potential to use an effective method of contraception during treatment with SARCLISA and for 5 months after the last dose [see Use in Specific Populations (8.1, 8.3)]. The combination of SARCLISA with pomalidomide or lenalidomide is contraindicated in pregnant women because pomalidomide or lenalidomide may cause birth defects and death of the unborn child. Refer to the pomalidomide or lenalidomide prescribing information on use during pregnancy.
1358104 Colloidal copper sulphide compositions INSTYTUT PRZEMYSLU FARMACEUTYCZNEGO 30 Sept 1971 [30 Sept 1970] 45523/71 Heading A5B [Also in Division C1] An aqueous solution of a high molecular weight substance and a copper salt is reacted with a sulphide to cause precipitation of cupric sulphide and formation of a colloidal cupric sulphide solution, the copper ion concentration in the solution being 0.1 to 2mg Cu per ml, reaction being carried out at from 0-5‹C. An alkali metal carbonate or bicarbonate may be included in the preparation in stoichiometric quantity relative to the copper salt. The colloidal solution, which may have a therapeutic use, may be prepared by forming an aqueous solution of a high-molecular weight substance such as peptone, gelatin, or a polysaccharide (preferably dextran) with addition of an antiseptic e.g. 0.5% phenol and dividing it into two equal parts: to one part is added a copper salt so as to obtain a concentration of 0.12-2 mg Cu/ml, and to the other part is added a stoichiometric amount of a carbonate or bicarbonate of an alkali metal and the solution is saturated with H 2 S. Both solutions are cooled to 0-5‹C for 12 to 48 hours and mixed, H 2 S being passed in to saturate the solution, preferably in an oxygen-free atmosphere. Excess H 2 S is removed by blowing in oxygen-free nitrogen or by reduced pressure. The solution is filtered, the pH adjusted to 6-7 and then made up into ampoules under nitrogen. To obtain a more concentrated solution the cupric sulphide containing excess H 2 S is divided into two equal parts: one part is freed from excess H 2 S as above, and a copper salt added while cooling to 0-5‹C in order to bring the Cu concentration to 5-10 mg/ml. To the other part a sol stoichiometric amount of an alkali metal carbonate or bicarbonate is added, the solution saturated with H 2 S, the two parts mixed while H 2 S is passed through and the mixture allowed to stand under H 2 S for 12-48 hours at 0-5‹C. Excess crystalloids in the copper containing solution may be removed prior to mixing with the H 2 S saturated carbonate or bicarbonate solution by dialysis. An alternative method of preparation is also described. The preparation may be used parenterally and particularly intramuscularly.
Sequence convergence, otherwise stereotypy, of B-cell receptor immunoglobulin (BcR IG) from unrelated patients is a distinctive feature of the IG gene repertoire in chronic lymphocytic leukemia (CLL) whereby patients expressing a particular BcR IG archetype are classified into groups termed stereotyped subsets. From a biological perspective, the fact that a considerable fraction (∼41%) of patients with CLL express (quasi)identical or stereotyped BcR IG underscores the key role of antigen selection in the natural history of CLL. From a clinical perspective, at odds with the pronounced heterogeneity of CLL at large, patients belonging to the same stereotyped subset display consistent clinical presentation and outcome, including response to treatment, likely as a reflection of consistent biological background. Many major stereotyped subsets were recently shown to have satellites, that is, smaller subsets that are immunogenetically similar. Preliminary evidence supports that this similarity extends to shared biological and even clinical features, with important implications for patient stratification. Consequently, BcR IG stereotypy emerges as a powerful tool for dissecting the heterogeneity of CLL toward refined risk stratification and, eventually, more precise therapeutic interventions.
AIM: To assess the thyroid allostasis in drug-free patients with affective disorder.
METHODS: Patients with major depressive disorder or bipolar disorder as drug-free, defined as those without psychiatric drugs exposure for at least 4 months before admission, from a tertiary hospital were recruited in this cross-sectional study. The primary outcomes were "structure parameters of thyroid homeostasis", which include "thyroid's secretory capacity" (SPINA-GT), "sum step-up activity of deiodinases" (SPINA-GD), the ratio of total to free thyroxine and "thyroid homeostasis central set point" (TSH index and "thyroid feedback quantile-based index" [TFQI]), calculated by TSH and thyroid hormones measured at admission. A healthy population and non-affective psychiatric disorder (schizophrenia) from the same catchment area were recruited as two comparison groups.
RESULTS: A total of 1263 cases of major depressive disorder, 1619 cases of bipolar disorder, 1186 cases of schizophrenia, and 162 healthy controls were included in the study. Compared to healthy control, GD and ratio of total to free thyroxine were lower in affective disorders. Bipolar with mania episode had higher GT than bipolar with depressive episode and major depressive disorder (median level at 3.70 vs. 3.04 and 3.03, respectively). Compared with healthy control, schizophrenia had higher TSH index and TFQI, but no increase in these parameters in major depressive disorder and bipolar disorder.
CONCLUSION: Affective disorders have a unique profile of thyroid allostasis with impaired step-up deiodinase activity and reduced serum protein binding of thyroid hormones, but no change in thyroid homeostasis central set point. Mania episode may be associated with higher thyroid secretory capacity.
The present invention relates to an HER2 targeting peptide molecule and a use thereof. The HER2 targeting peptide molecule has the following general formulas: X-peptide, X-L-peptide, peptide-X, and peptide-L-X, wherein X represents a mark unit for a report function (fluorescence, diagnostic radionuclides, nuclear magnetic resonance, etc.) or a therapeutic function (therapeutic radionuclides, chemical drugs, etc.), L represents a linking group, peptide represents a unit derived from a polypeptide Herceptide, and the amino acid sequence of the polypeptide Herceptide is as shown in SEQ ID No.: 1. The HER2 targeting peptide molecule in the present invention can specifically recognize and target an HER2 receptor, can be effectively gathered and retained at a tumor site, and can be used for tumor targeted diagnosis and treatment.
BACKGROUND: Hemocompatibility-related adverse events affect patients after left ventricular assist device (LVAD) implantation but are hard to predict.
OBJECTIVES: Dynamic risk modeling with a multistate model can predict risk of gastrointestinal bleeding (GIB), stroke, or death in ambulatory patients.
METHODS: This was a secondary analysis of the MOMENTUM 3 (Multicenter Study of MagLev Technology in Patients Undergoing Mechanical Circulatory Support Therapy with HeartMate 3) trial. HeartMate 3 LVAD recipients who survived to hospital discharge and were followed for up to 2 years. A total of 145 variables were included in the multistate model with multivariate logistic regression. Model performance was assessed with the area under the curve in a holdout validation cohort. A risk stratification tool was created by dividing patients into categories of predicted risk using the final model variables and associated OR.
RESULTS: Among 2,056 LVAD patients, the median age was 59.4 years (20.4% women, 28.6% Black). At 2 years, the incidence of GIB, stroke, and death was 25.6%, 6.0%, and 12.3%, respectively. The multistate model included 39 total variables to predict risk of GIB (16 variables), stroke (10 variables), and death (19 variables). When ambulatory patients were classified according to their risk category, the 30-day observed event rate in the highest risk group for GIB, stroke, or death was 26.9%, 1.8%, and 4.8%, respectively. The multistate model predicted GIB, stroke, and death at any 30-day period with an area under the curve of 0.70, 0.69, and 0.86, respectively.
CONCLUSIONS: The multistate model informs 30-day risk in ambulatory LVAD recipients and allows recalculation of risk as new patient-specific data become available. The model allows for accurate risk stratification that predicts impending adverse events and may guide clinical decision making. (MOMENTUM 3 IDE Clinical Study Protocol; NCT02224755).
Variants of an immunoglobulin variable domain, said immunoglobulin variable domain comprising (A) at least one CDR region and (B) framework regions flanking said CDR, said variant comprising: (a) said CDR region havind added or substituted therein at least one binding sequence and (b) said flanking framework regions, wherein said binding sequence is heterologous to said CDR and is a binding sequence from a binding site of a binding pair, and wherein said binding sequence is a receptor-binding portion of angiogenin. Additionally, amino acid residues in the flanking and/or non-flanking framework regions may have been deleted, substituted or inserted. Also claimed are molecules comprising said variants. Said molecules may be immunoglobulins. Nucleic acids encoding said variants, and cells and recombinant non-human hosts containing said nucleic acids. Pharmaceutical compositions comprising an effective amount of said variants. Vaccines comprising an effective amount of said variants, especially to induce an anti-idiotype response. Method of treating or preventing a disease using an effective amount of said variants or of the nucleic acids encoding said variants.
PURPOSE:To obtain a pigment dispersion liquid usable as a hair cosmetic having improved dispersibility of pigment and excellent dispersion stability, by using a specific polyoxyalkylene compound and a pigment as essential components. CONSTITUTION:The objective dispersion is produced by compounding (A) a polyoxyalkylene compound produced by the addition polymerization of a monohydric or polyhydric alcohol and an alkylene oxide, preferably propylene oxide (PO) or a mixture of PO and ethylene oxide (EO) (the total molar number of added alkylene oxide is 2-100, especially 8-50mol.) with (B) a pigment having a particle diameter of preferably 0.005-100mum, especially 0.01-10mum. The amount of the component A is 1-50wt.% and that of the component B is 0.005-10wt.%, especially 0.01-1wt.%. The alkylene oxide in the component A is especially preferably PO. In the case of using PO Mixed with EO, it is preferable to suppress the addition molar number of EO to <=1/3 of the total molar number and to carry out the addition polymerization to form a block polymer.
All patients receiving in scheme 1 and scheme 2 will be tested using the standard tests used in the study context. These are rapid HIV tests, Xpert MTB/RIF and culture.
Ultraviolet (UV) B-induced damage in human epidermal keratinocytes (HEKs) initiates photocarcinogenesis. However, how diabetes influences photocarcinogenesis is not well understood. To investigate the impact of high-glucose environments on responses to UVB, we cultured HEKs in normal-glucose (NG) or high-glucose (HG) conditions (G6 and G26), followed by UVB irradiation at 25 mJ/cm2 (G6UVB and G26UVB). We performed next-generation sequencing and analyzed HEKs' expression profiles bioinformatically to identify candidate genes and cellular responses involved. We found UVB induced consistent responses in both NG- and HG-cultivated HEKs, but it also triggered certain distinct processes and pathways specifically in the HG groups. The 459 differentially expressed (DE) genes in the HG groups revealed their roles in chromatin remodeling, nucleosome assembly, and interferon signaling activation. Moreover, the 29 DE genes identified in G26UVB/G6UVB comparison, including the potent tumor suppressor gene TFPI2, were considered key genes contributing to HEKs' altered response to UVB in HG environments. UVB irradiation induced significantly higher TFPI2 expression in HG-cultivated HEKs than their NG-cultivated counterpart. Finally, HG-cultivation significantly increased oxidative stress, cyclobutane pyrimidine dimer formation, and apoptosis, while reducing HEKs' viability after UVB irradiation. These changes under HG conditions probably mediate cell fate toward death and tumor regression. Overall, our findings provide evidence and associated molecular basis on how HG conditions reduce keratinocytes' photocarcinogenic potential following UVB exposure.
The purpose of this study was to investigate the effects of BOC on glioblastoma cells and its underlying mechanisms. In vitro, BOC-knockdown was performed in glioma cell lines. CCK-8 and Transwell were used to assess the impact of BOC on the viability, invasion, and migration of gliobma cells. RNA-seq technology was employed to analyze the differential gene expression between BOC-knockdown glioma cells and the control group, and qRT-PCR was used to validate the expression of downstream differential genes. SMO-overexpression was performed to investigate the effects of SMO on glioma cells. A BOC-knockdown mouse subcutaneous tumor model was to verify the effects of BOC on mouse tumors. Tissue microarray technology was used to detect the expression of BOC and SMO in samples of normal human brain tissue and glioma tissue. In vitro, BOC-knockdown inhibited the viability, invasion, and migration of glioma cells, as well as downregulated the expression of downstream differential genes SMO, EGFR, HRAS, and MRAS. Conversely, SMO-overexpression upregulated the viability, invasion, and migration abilities of BOC-knockdown cells. In vivo, BOC-knockdown suppressed tumor growth in mice and downregulated the expression of downstream differential genes SMO, EGFR, HRAS, and MRAS. Tissue microarray results showed that both BOC and SMO were highly expressed in glioma tissues. BOC is aberrantly overexpressed in glioma patients and promotes glioma development. Mechanistically, BOC activates the Hedgehog (Hh) and RAS signaling pathways by upregulating the expression of SMO, EGFR, HRAS, and MRAS, thereby facilitating the Proliferation, invasion and migration of glioma cells.
ATRA will be administered per os 1 week before the start of (chemo)radiotherapy (daily administration for 3 days). In addition of radiotherapy, patient will receive cisplatin or cetuximab (standard of care treatments).Then, patient will receive 6 to 8 weeks of standard (chemo)radiotherapy. Subsequently, ATRA will be administered per os for 3 days every 3 weeks for a total of 4 courses starting 2 to 3 weeks after the end of (chemo)radiotherapy. Finally, the patient will be followed until document progression disease, death, withdrawal of consent or end of trial (whichever occurs first).
OBJECTIVE: Re-resection of spinal giant cell tumors is an exceedingly difficult procedure. Moreover, the prognosis of patients with en bloc resection or intralesional excision for re-resection has rarely been reported. This study aimed to compare the prognostic value of en bloc resection with that of intralesional excision in patients undergoing re-resection for giant cell tumors of the spine.
METHODS: This retrospective analysis evaluated patients who underwent revision surgeries for relapse of giant cell tumors of the spine at our center between January 2005 and January 2021. Local progression-free survival represents the duration between en bloc resection or intralesional excision and tumor recurrence. Neurological recovery, survival rates, local control, and complications were evaluated. The Kaplan-Meier estimator was used for survival analysis.
RESULTS: A total of 22 patients (nine men and 13 women) with a mean age of 34.1 (range 19-63) years were included. Significant statistical differences were found in the local tumor recurrence rate between patients treated with en bloc resection and those treated with intralesional excision (p < 0.05). The 5- and 10-year local progression-free survival rates were both 90% in the en bloc resection group, while in the intralesional excision group, the 5-year local progression-free survival rate was 80% with a 10-year rate of 45.7%. The en bloc resection group had a lower local tumor recurrence rate than that of the intralesional excision group (p < 0.05), but the former had a higher rate of complications (p = 0.015).
CONCLUSIONS: This study revealed a low local recurrence rate in patients who underwent en bloc resection for giant cell tumors, while the perioperative complication rate was high.
Chronic inflammation is the gate of many human illnesses and happens when the immune system is unable to suppress external attacks in the correct form. Nonetheless, the gut microbiome plays a pivotal role in keeping homeostasis in the human body and preventing inflammation. Imbalanced microbiota and many diseases can result in inflammation, which when not taken seriously, can be turned into chronic ones and ultimately lead to serious diseases such as cancer. One approach to maintaining hemostasis in the human body is consumption of probiotics as a supplement. Probiotics impact the immune functions of dendritic cells (DCs), T cells, and B cells in the gut-associated lymphoid tissue by inducing the secretion of an array of cytokines. They activate the innate immune response through their microbial-associated molecular pattern, and this activation is followed by multiple cytokine secretion and adaptive elicitation that mitigates pro-inflammatory expression levels and tumor incidence. Thus, according to several studies showing the benefit of probiotics application, alone or in combination with other agents, to induce potent immune responses in individuals against some inflammatory disorders and distinct types of cancers, this review is devoted to surveying the role of probiotics and the modulation of inflammation in some cancer models.
A middle-aged man presented with inferior wall ST-elevation myocardial infarction and underwent primary percutaneous coronary intervention with tirofiban administered due to extensive thrombus. He developed sudden-onset dyspnoea, bilateral crepitations, haemoptysis, desaturation and hypotension an hour after starting tirofiban infusion. The tirofiban, antiplatelet medications and heparin were stopped immediately. Chest X-ray showed patchy opacities in the left upper, middle and lower zones. High-resolution CT showed confluent areas of consolidation with surrounding ground glass opacities and interlobular septal thickening (crazy pavement appearance) representing diffuse alveolar haemorrhage (DAH). He was managed with inotropes, non-invasive ventilation and intravenous furosemide. He was asymptomatic with complete resolution of lung opacities in chest X-ray done 2 months follow-up. DAH is a rare but potentially life-threatening complication which is often misidentified with other respiratory syndromes. Treatment includes stopping tirofiban and anticoagulant medication, blood transfusion, and institution of mechanical ventilation.
A traditional Chinese medicine composition used for treating viral myocarditis is disclosed. The composition comprises following twenty seven medicines: cortex acanthopanacis, rheum officinale, willow herb, lily, gorgon fruit, paper mulberry fruit, radix astragali, Chinese angelica, red ginseng, licorice, ciliate desert-grass, fleshyleaf lysionotus herb, thickleaf croton root, Chinese toona fruit, coltsfoot flower, kadsura pepper stem, sweet wormwood, tuber fern, lespedeza bicolor, turnip seed, folium pyrrosiae, corn stigma, clematis, rhizoma nardostachyos, capsella bursa-pastoris, ziziphora clinopodioides lam. and burdock. The viral myocarditis belongs to febrile diseases in the field of traditional Chinese medicine. The diseased region of the viral myocarditis is heart mainly and relates to lung, spleen, stomach, and other internal organs. The etiology and pathogenesis are mainly insufficient healthy qi of bodies for long term, deficiency of yin in heart and lung and heart invasion by pathogenic toxins. The composition is based on the integral diagnosis and treatment theory in the traditional Chinese medicine. Targeting at the etiology and pathogenesis of the viral myocarditis, the composition adopts rules of treatment of clearing away heat and toxicity, promoting blood circulation to remove meridian obstruction, purging intense heat to expel stasis, and tonifying qi and yin, is capable of eliminating pathogenic factors, regulating yin, yang, blood and qi of a body, and promoting vital energy, and has good curative effects on the viral myocarditis.