Supplementation in the form of whey protein, creatine and amino acids have all been shown to be a potent stimulus for muscle hypertrophy, when combined with resistance exercise. Burke et al. (2001) reported that males who supplemented with a combination of whey protein and creatine had greater increases in lean tissue mass and strength than those who supplemented with only whey protein or placebo. Hulmi at al. (2010) identified the most important component of whey to be its high concentration of the branched chain amino acid leucine, a critical amino acid for increasing muscle protein synthesis and suppressing muscle protein breakdown. The combination of leucine and whey protein has been shown to promote significant increases in muscle cross-sectional area. Vitamin D and calcium citrate have also been shown to enhance increases in muscle strength and lean mass, albeit in a permissive rather than stimulatory manner. Protein supplements have consistently been shown to promote greater gains in hypertrophy than their placebo counterparts, however these placebo supplements are often isoenergetic carbohydrate-based formulas. Few studies have compared two supplements with an equal amount of protein and amino acids, or have combined multiple beneficial ingredients to form a multicomponent supplement to promote greater gains in lean body mass and strength. Finally, sex differences in resistance training programs have rarely been studied, less so when testing a new supplement. Thus, the purpose of this study will be to determine whether there is an augmented effect of our nutritional supplement on the degree of hypertrophy in young male and female adults involved in a resistance training program. This nutritional supplement will be compared with a placebo containing an equivalent amount of collagen protein and non-essential amino acids. A secondary analysis will assess sex-based differences in strength and muscle size. Based on previous literature, we hypothesize that the combinatorial effect of whey protein, leucine, creatine, calcium citrate and vitamin D will induce hypertrophy to a larger degree than resistance exercise with the placebo in young adults.

A synergistic combination of ingredients with known benefits will be given twice daily to healthy young men and women (18-30 years old), (n = 22) in conjunction with a 12-week undulating periodized resistance training protocol, in which the repetition number and intensity of the training sessions will vary. Notable ingredients in this supplement include whey protein isolate (20g), leucine (2g), creatine monohydrate (2.5g), calcium citrate (300mg) and vitamin D (1000IU). The placebo condition, also comprised of males and females (18-30 years old), (n=22) will ingest a formulation containing an equivalent amount of collagen protein (20g), as well as an equivalent amount of amino acids (alanine, 1.4g and glycine, 0.6g). Pre- and post- assessments will include dual-energy x-ray absorptiometry (DEXA), one-rep maximum (1RM), and an ultrasound of the quadriceps. Four muscle biopsies will be taken from the vastus lateralis: 1) at rest prior to training, 2) 48h after an acute bout of exercise prior to training, 3) at rest post-training, and 4) 48h after an acute bout post-training. This allows for comparisons of acute and trained effects of both conditions. Using novel immunohistochemical staining procedures we will examine the resistance exercise-induced changes in fibre type distribution and fibre cross-sectional area in the supplement and placebo conditions as well as perform sex-based comparisons.

The novel aspect of this study will be the comparison of two isoenergetic, isonitrogenous supplements as stimulants for muscle protein accretion, as opposed to using carbohydrates as a placebo. The supplement will contain a new formulation of ingredients yet to be studied together. The synergistic effect of these ingredients will attempt to maximize muscle growth in both males and females, meaning that associations between sex and exercise can be assessed. This research will help us identify a new method to augment resistance exercise training through protein, amino acids, vitamins and minerals as well as expand the literature surrounding the effects of these ingredients on muscle hypertrophy.