We want to use the product Dermatix or some equivalent product to 1) try to proliferate stem cells and 2) use it for scarred tissue inside the body a procedure done before grafting or adding or scaffolding stem cells (eg. into a spinal chord injury patient) so the stem cells aren't blocked from taking to injured wound.     Taken from Dermatix website:  The Innovation Dermatix.TM. Gel is a patented proprietary formula containing bio-inert and biocompatible silicone compounds, namely polysiloxane, silicon dioxide and nonvolatile silicone components. It uses basic long-chain polymers as topical silicone-gel sheeting. The result is a waterproof, gas-permeable membrane that acts like an extra layer of skin. It helps to soften and flatten the scar and to reduce pain, itching and scar-associated discoloration, while maintaining the moisture balance and elasticity of the adjacent skin. We are looking at running electrode through stem cell colonies to deliver electrical impulses to the stem cells in the hope of continuing the stem cells proliferation.   We also plan to use Netrin-1 protein 1) harness and study its effects to facilitate the stem cells to repair itself to and use to cause stem cells to proliferate 2) and cause the stem cells to convert into axons for spinal chord and brain injuries 3) we could also use it in conjunction stem cell injections.     Other Topical Ointments include:   Retin-A (tretinoin) perhaps in combination with hydroquinone and cortisone cream and as well alpha hydroxy acids and vitamin C serums and possibly steroids such as triamcinolone.  We are using Helix Aspera Muller Glycoconjugates snail/slug slime to enhance proliferation of stem cells. It is comprised complex sugar molecules bound to proteins, peptides, enzymes, coenzymes, oligoelements that play a role in messengers's communication between cells moderators of inflammatory reaction when skin if damaged, and regulators of fiberoblast proliferation and functions of the skin matrix and new connective tissues such as collagen and elastin fibers and water holding molecules (glycosaminoglycans and proteoglycans.     The Below is Taken BioSkincare Website:   FACTORS THAT HELP TO RECOVER THE NORMAL PHYSIOLOGY OF THE SKIN.  ~ Low molecular weight proteins that activate Fibroblast Growth Factor activity. Fibroblast proliferation is key for they are the stem cells in the basal membrane of the skin that give rise to new connective tissues: collagen and elastin fibers plus the glycosaminoglycans and proteoglycans that act as the glue that bind cells together and as fillers with a large capacity to withhold many times their weight in water and give skin strenght, elasticity and the capcity to withstand overstretching without breaking of skin fibers.   ~ High molecular weight proteins that work as Oxygen carriers (copper-haemocyanin) ~ Copper peptides that induce the degradation of "extra-large" collagen aggregates found in scar tissues such as those in stretch marks, and promotes the synthesis of smaller more regular collagen as is normal skin ~ Enzymes, both with Collagenase and Gelatinase activities that degrade damaged proteins and scar tissues ~ Hyaluronic Acid, provides deep tissue hydration ~ Glycoproteins / mucopolysaccharides that act as signaling molecules and enhance communication among and within cells, thus coordinating an orchestrated process that recognizes damaged and scar tissues, and activates the enzymes that "digest" or further degrade them into their amino-acid and other components, and uses those same components for the reconstruction of the extracellular matrix ~ Trace elements (Copper, Zinc, Iron, Calcium), some acting as coenzymes, necessary for Cellular Renewal, ~ Low molecular weight antioxidant compounds with Antioxidant & Anti-inflammatory effects.   We also plan to use it in conjunction with (prior to) injecting stem cells into scarred injury (eg.  scarred tissue) anywhere where there is scarred tissue.   After removing the scarring, and adding stem cells to the injured area of the spinal chord, we use netrein-1 then we apply electrodes to prime the stem cells to grow into stem cells.   Additionally we could use detectors for faint muscle signals (initially with robotic help the limb's movement) then as the signal gets stronger, by retrains the brain signalling (neuron sounds or other form or mental activity detector) we could add neuron muscle patches that strengthens the muscle and eventually turn down the strength of the nerve patch signal and teach the patient to concentrate to increase the brains and nerve signals that operate the limb movement manually.   Furthermore, we will treat the stem cells with retinoic acid and vitamin A and (different concentrations of) frizzled WNT (G proteins), (sonic) hedgehog (agonists - Hh protein, Hh-Ag1.1, Hh-Ag1.2, vehicle (DMSO) pathways SAG, purmorphamine (smoothened) and nerve growth factor (NGF), brain derived neutrophic factor (BDNF), neutrophin-1 (NT-1), neutrophin-2 (NT-3), neutrophin-4 (NT-4) and possibly other growth factors. We also are looking at transcription factors Pax7, MNR2Nkx2.2.  We culture stem cells in dibutyrl camp (dbcAMP), then we add rolipram, GDNF, and with motor neurons and neurons and different types of interneurons (as converted to stem cells by differing concentrations of sonic hedgehog). Dr. Kerr.   Breaks that need new bridging... the sciatic nerve extends from the spine down the back of the hind leg, and extending into the peripheral nervous system and all the way down to the gastronemius muscle in the lower leg and formed functional connections called synapses, with the muscles. Dr, Kerr.   We are also developing ways to observe cells innards via micro (real time x-ray, Cat Scan, fMRI, MRI or micro video probe - in REAL TIME 1) Lower Power to see through the cell membrane to see the cytoplasm and organelles eg mitochondria, 2) Medium Power to see through the mitochondria and 3) High Power to see through the nuclear membrane). The idea in this case is to sort the types of neural cells at a stem cells early stage. We are initially looking setting up an assembly line of colonies that the micro video camera observes to determine the different cells we look for types by shapes, size (of cells and organelles) positioning/orientation and placement (where suspended), conductivity (effects of electro pulse on different types of cells) of innards to their DNA orientation, make up of cytoplasm and percentage relative size of eg. cytosol to mitochondria (numbers), protrusions on the cellular membrane (receptors) - eg. dendrites are shown to have axons (but are they there in early stage of development...).   Then there is the question of do we need to separate the neural cells if the cells are used for spinal cord injury.