Study Design The study is a Randomized controlled clinical trial. 3.2 Study Setting The study was carried out in the department of Periodontology at Dr.Ishrat-ul-Ebad Khan Institute of Oral Health Sciences.

3.3 Study Duration The duration of the study was 6-8 months after the synopsis approval. 3.4 Study Population The study was conducted on patients with Chronic Periodontitis. 3.4.1 Inclusion Criteria

1. Male/ female, 35-65 years of age with minimal 5 natural teeth in each quadrant excluding 3rd molars.
2. Patients having Chronic Periodontitis with probing depth 4 to 6mm and bleeding on probing in 20 sites.
3. Systematically healthy individuals with Chronic Periodontitis. 3.4.2 Exclusion Criteria

1. Individuals who smoke. 2. Any addiction of Areca nuts, gutka, paan or alcohol. 3. Individuals with any systemic diseases like diabetes mellitus, cardiovascular, endocrine as they may affect the outcome of research.

4. Women with pregnancy/lactation. 5. Individuals with any known allergy from aloe-vera or honey. 6. Systemic or topical use of antibiotic in past 6 months. 3.5 Sample Size Total of 105 patients were calculated (35 in each group) by using PASS V-11, Pair sample T-test with 95% Confidence Interval and 80% Power of test. Difference of plaque index at base line versus after 1 month mean (20.02) and standard deviation (68.16). Calculated sample size is 93, which is increased to 105.(26) 3.6 Sampling Technique The sampling technique was Non Probability consecutive sampling. 3.7 Data Collection Procedure Patients attending the Department of Periodontology were approached and examined for chronic periodontitis. Patients with chronic periodontitis were explained the research study procedure. Patient signing the consent form was enrolled in the study. All the enrolled participants went through a detailed history which includes medical health, systemic illness, regular medications, hospitalizations, any known allergies, dental checkups, any bad oral habit and routine oral hygiene practice. Any entrant having any allergy from honey or aloe-vera gel or anyone falling in our exclusion criteria were not selected in our research. All those who fulfilled our criteria were clinically examined and all the clinical parameters were recorded by the principal investigator at the baseline, i-e, pocket depth, gingival index, plaque index and bleeding on probing. Probing depth was recorded by Goldman-Fox probe with William's markings and flattened surface. Probing depth measurement was done by inserting probe on six sites on each tooth (mesial, distal, mid lingual, mid buccal, buccal and lingual), all the markings were measured from the marginal gingiva to the base of the periodontal pocket and were recorded in millimeters, if the tooth has recession and clinical attachment loss in any site of tooth it was not measured and was not included in our study. Bleeding on probing was checked on the buccal and lingual sulcus of tooth after inserting the probe and wait for 30 seconds, if bleeding occurs bleeding on probing was considered as 1 and labeled as positive bleeding on probing and if there is no bleeding it was considered as 0 and labeled as no bleeding on probing, Percentage of BOP was recorded by dividing positive bleeding sites by total number of sites present in each patient multiplied by 100. Gingival index (Loe and Silness, 1963) were recorded as score 0: Normal gingiva, 1: Mild inflammation on gingiva, 2: Moderate inflammation on gingiva and 3: Severe inflammation on gingiva. Plaque index (Loe and Silness, 1964) was recorded as score 0: No plaque, 1: plaque film on free gingival margin, 2: Moderate plaque on gingival margin; can be seen by the naked eye, 3: Abundance of soft matter in gingival pocket. After recording all the clinical parameters principal investigator performed non-surgical therapy (scaling and root debridement) by using ultrasonic scalers in single visit. Following scaling and root debridement the participants of all the three groups were given detailed oral hygiene instructions with demonstration of proper brushing technique i-e Modified Bass technique. After that all the participants were provided with a regular cleaning kit which consist of fluoride toothpaste (Colgate® Cavity Protection Toothpaste) and toothbrush (Colgate® Classic Clean Toothbrush) Moreover, all enrolled patients in Group A and B were minutely demonstrated by principal investigator for gingival massage.

Aloe-Vera gel Production Aloe vera gel used in this study was 98% aloe vera gel concentration and 2% normal saline. Preparation of aloe-vera gel consists of ripen leaf of aloe vera.(26) Aloe vera leaf was washed thoroughly under water and then cut from the center and the seeping gel was collected in sterile container and was stored at 4°C until the time of application. In our research this pure Aloe vera gel was extracted by The Pakistan Council of Scientific \& Industrial Research (PCSIR) laboratory.

Honey collection Sidr (Beri) honey was used in this study. Honey was thick, raw and cold extracted from remote Pakistan afghan tribal forest belt of Sidr trees. It was smooth, viscous and medium to dark amber in color.

Random Allocation of the Intervention A total of 105 participants were divided in to three groups and were named as Group A: SRD \& Gingival massage with Aloe-Vera gel, Group B: SRD \& Gingival massage with Sidr honey and group C: SRD only. Interventions were randomly allocated. For this purpose, entrants were asked to pick one sealed opaque envelope in which random allocation of the interventions were mentioned. All the participants who were in Group A and B were not blinded and trained for gingival massage by principal investigator. All the participants were guided to rub aloe-vera gel or sidr honey on the skin of wrist first to recheck any allergy. At last participants were trained for application of the material gently on the gingiva and the gingival sulcus of all the teeth. To perform gingival massage patients used the index finger of their dominant hand, applied the material at the bottom of their gums with slight pressure, then moved the finger in a slow and circular motion/rubbing action for 1 to 3 minutes, after performing the massage patients were not allowed to rinse with water or any other fluids for 30 minutes for further retaining the material on the gingiva and in the gingival sulcus thereby preventing washing out of the material. All the patients were instructed to perform gingival massage with aloe-vera gel and sidr honey twice a day after meals and to wait for 30 minutes after application of the interventions. All the routine oral hygiene practice which includes brushing twice a day (morning and night) was continued as usual. Patients falling in the control group were instructed to follow routine oral hygiene protocols. Telephonic communications were made weekly for encouraging the patients for gingival massage with given interventions. All the patients were recalled on 30th day for Post-intervention assessment. The baseline clinical parameters were recorded by principal investigator whereas 1 month follow-up of all clinical parameters were recorded by a trained examiner in this study. Intra-examiner reliability was checked to ensure calibration between examiners. For this purpose both examiners recorded clinical parameters of 50 subjects with chronic periodontitis who were not involved in our study and reliability came out to be 88%. The trained examiner who was blinded from the intervention given to patients was asked to re-assess all the clinical parameters which included periodontal pocket depth, gingival index, plaque index and bleeding on probing.