anthonyyazdaniml/gliner-biomed-curated-corpus huggingface dataset:
BACKGROUND: Increasing studies have shown degeneration of nucleus pulposus cells (NPCs) as an critical part of the progression of intervertebral disc degeneration (IVDD). However, there are relatively few studies on single-cell transcriptome contrasts in human degenerated NPCs. Moreover, differences in Wnt/Ca2+ signaling in human degenerated nucleus pulposus cells have not been elucidated. The aim of this study is to investigate the differential expression of Wnt/Ca2+ signaling pathway between normal and degenerated nucleus pulposus cells in humans and try to investigate its mechanism.
METHODS: We performed bioinformatics analysis using our previously published findings to construct single cell expression profiles of normal and degenerated nucleus pulposus. Then, in-depth differential analysis was used to characterize the expression of Wnt/Ca2+ signaling pathway between normal and degenerated nucleus pulposus cells in humans.
RESULTS: The obtained cell data were clustered into five different chondrocytes clusters, which chondrocyte 4 and chondrocyte 5 mainly accounted for a high proportion in degenerated nucleus pulposus tissues, but rarely in normal nucleus pulposus tissues. Genes associated within the Wnt/Ca2+ signaling pathway, such as Wnt5B, FZD1, PLC (PLCB1), CaN (PPP3CA) and NAFATC1 are mainly present in chondrocyte 3, chondrocyte 4 and chondrocyte 5 from degenerated nucleus pulposus tissues. In addition, as a receptor that activates Wnt signaling pathway, LRP5 is mainly highly expressed in chondrocyte 5 of degenerated nucleus pulposus cells. Six genes, ANGPTL4, PTGES, IGFBP3, GDF15, TRIB3 and TNFRSF10B, which are associated with apoptosis and inflammatory responses, and are widespread in chondrocyte 4 and chondrocyte 5, may be closely related to degenerative of nucleus pulposus cells.
CONCLUSIONS: Single-cell RNA sequencing revealed differential expression of Wnt/Ca2+ signaling in human normal and degenerated nucleus pulposus cells, and this differential expression may be closely related to the abundance of chondrocyte 4 and chondrocyte 5 in degenerated nucleus pulposus cells. In degenerated nucleus pulposus cells, LRP5 activate Wnt5B, which promotes nucleus pulposus cell apoptosis and inflammatory response by regulating the Wnt/Ca2+ signaling pathway, thereby promoting disc degeneration. ANGPTL4, IGFBP3, PTGES in chondrocyte 4 and TRIB3, GDF15, TNFRSF10B in chondrocyte 5 may play an important role in this process.
This is a single center, observational real-world study recruiting myasthenia gravis patients from Neurology Departments of Xuanwu Hospital, aiming to compare effectiveness and safety of 2 different inmunotherapy for MG. The study plans to recruit 160 MG participants and divides into 2 treatment groups according to physician's judgment and preferences of patients, one is combined immunotherapy group in which tacrolimus added with low-dose prednisone (0.25mg/kg/d), and the other is tacrolimus monotherapy group. Both groups can be treated with pyridostigmine to relieve symptoms. Patients are followed up at 1, 3 and 6 month after treatment initiation to assess the efficacy of both regimen. The primary outcome is the change of MG-ADL scores. Also, liver and renal functions are tested to monitor any side effects. Patients' clinical records are uploaded to an online database.
A PROCESS FOR THE PREPARATION OF CHYMOSIN                  ABSTRACT          There is described a process for the preparation of chymosin in which an insoluble form of a chymosin precursor, such as methionine-chymosin, is produced by a host organism, such as Saccharomyces cerevisiae or E.coli, transformed with a vector including a gene coding for the chymosin precursor,  wherein the insoluble  form of the chymosin precursor is solubilised to produce a soluble native form of the chymosin precursor prior to cleaving the soluble native form of the chymosin precursor to produce chymosin.    The  solubilisation  involves  denaturing the insoluble  chymosin  precursor  and then  allowing  it  to renature forming the normal or native form of the chymosin precursor.   The native form may then be cleaved to form chymosin.  The denaturants used include aqueous solutions of urea or guanidine hydrochloride and alkaline solutions of pH between pH 9.5 and pH 11.5 either separately or in combination.    In  a  particular type  of  colubilisation described, the insoluble chymosin precursor is denatured using a urea or guanidine hydrochloride solution.   The resulting  solution  is then  diluted  into  an  alkaline solution  to  yield  the  native  form  of  the  chymosin precursor.
Cerebral ischemic stroke ranks among the leading causes of death and disability worldwide. A significant challenge, beyond the lack of effective therapies, is the frequent oversight of sex as a vital factor in stroke research. This study focuses on elucidating the sex-specific epigenetic mechanisms that contribute to neural damage and recovery in cerebral ischemia. In our previously reported study, we demonstrated that following ischemia-induced cerebral artery occlusion (ICAO), female striatal tissue exhibited an early reinstatement of H3K9me2 marks on the promoters of inflammatory genes compared to male striatal tissue. This restoration led to a reduction in the expression of inflammatory cytokines, ultimately contributing to accelerated recovery in females. Building upon these findings, the current study aimed to investigate the unidentified molecular pathways responsible for the accelerated recovery observed in females. To explore this, we performed illumina-RNA sequencing on striatal tissues 24-h post-ICAO. Interestingly, our analysis revealed differential regulation of H3K27me2 marks on the promoters of various neurogenic genes at an early stage, which facilitated early neurogenesis in the female striatum. This investigation identifies an epigenetic modulator, kdm6b/jmjd3, targeting H3K27, and delineates its sex-specific role in neural stem cell proliferation. The findings contribute to a comprehensive model linking gender-specific epigenetic regulation, neurogenesis, and post-ICAO recovery. In conclusion, the identified epigenetic modulators and their roles in neurogenesis offer potential targets for refined therapeutic interventions, emphasizing the importance of personalized and sex-specific considerations in stroke studies.
BACKGROUND: A few patients report intense pain and other unpleasant sensations, such as burning, dysesthesia and hyperalgesia, after even brief exposure to the sun and in the absence of any skin lesion. Sometimes they also develop systemic symptoms, such as mild fever, fatigue, faintness and fainting. As a result, these patients carefully avoid even short-term sun exposure with a consequent severe negative impact on their lives.
METHODS: We have reviewed the clinical findings and the results of photobiological investigations of 10 patients who presented this clinical picture. Six of these patients were previously described by our group with the diagnosis of sun pain. We have reviewed the similarities with other previously described disorders such as solar dysesthesia and PUVA pain and have evaluated possible pathogenetic mechanisms.
RESULTS: During phototesting our patients experienced intense pain in the exposed area and in the surrounding skin, without any visible lesion, even with very low sub-erythemal doses. At follow-up, five patients were diagnosed with fibromyalgia, three with a major depressive disorder, one with bipolar syndrome and one with a conversion disorder. The pathogenesis remains unclear, but the use of a psychopharmacological treatment with antidepressants improved both the neuropsychiatric symptoms and sensitivity to the sun in most subjects.
CONCLUSION: For patients with pain and other severe symptoms in the absence of skin lesions and clinical and laboratory manifestations of known photodermatoses, a neuropsychiatric evaluation should be suggested.
The invention discloses a tangerine peel cream and a manufacturing method thereof. The manufacturing method of the tangerine peel cream comprises the following steps: (1) the tangerine peel of one portion by the weight proportion is smashed by a stirring mill, 1.5 to 3 portions of water is added for cooking for 1.5 to 2.5 hours and the mixture is stirred continuously during the process of cooking; (2) tangerine peel dregs are separated, tangerine peel juice after the tangerine peel dregs are separated is slowly decocted by slow fire until the water is absorbed completely, and solidity tangerine peel cream is obtained; (3). the hot cream is molded by a mold; (4). the molded tangerine peel cream is dried by a drier, and hard lump shaped tangerine peel cream is manufactured by drying; (5). the hard lump shaped tangerine peel cream is packed by tinfoil firstly, and then the package is vacuumized. The tangerine peel cream of the invention can maintain the efficacies of regulating qi, promoting digestion, eliminating dampness and eliminating phlegm of the tangerine peel; the lumpy tangerine peel cream is more convenient for people to carry and store, which is a good product for journey; in addition, the tangerine peel cream has the advantages of good taste, purer fragrance, simple manufacturing process and being easy to realize, which can easily achieve the goal of being clean and sanitary and protecting the environment.
This was a follow-up study, investigational product was administered in the previous study. Participants in the BIIB033 arm have received at least 1 dose of 100 mg/kg BIIB033.
The utility model discloses traditional Chinese medicine tablets used for curing a plurality of cancers, and belongs to the field of traditional Chinese medicine products. The traditional Chinese medicine tablets are round film-coated tablets in shape. The diameters of the tablets are 9.0mm. The tablets comprise inner layers and outer layers. The inner layers comprise glaucescent fissistigma root extract, starch and dextrin; and the outer layers comprise stomach-soluble coating powder. Components of the inner layers include, by mass, 38%-42% of glaucescent fissistigma root extract, 50%-56% of starch and 2%-5% of dextrin. After film coatings are removed, the traditional Chinese medicine tablets with tiny odor are sepia in color and bitter in taste. The glaucescent fissistigma roots are the main ingredients of the traditional Chinese medicine tablets which can directly kill various cancer cells, restrain spread of the cancer cells, and are nontoxic and free of side effects. Meanwhile, the film coatings are adopted to form protective films on the surfaces of the tablets, so that contact between effective constituents in the tablets and air is blocked up, decomposing of medicinal constituent chlorogenic acid is prevented, stability of tablet quality is guaranteed, and the tablets are safe and effective to take.
Participants assigned to this group will receive Parents As Teachers Plus (PAT+). This will be a diet and physical activity lifestyle intervention integrated within the standard Parents As Teachers home visiting curriculum. There will be a total of 28 home visits, delivered over 24 months (6 month prenatal phase and 18 month post-partum phase).
The aim of this study is to investigate the effect of pes planus on physical activity level, exercise capacity and quality of life in young adults. There are studies examining the effects of pes planus on physical fitness, quality of life, balance and pain levels. However, there are no studies evaluating the effect of pes planus on exercise capacity and physical activity level. It will enable us to have information about its capacity and its effect on quality of life and to obtain workable data. Thanks to these data, the orientation towards treatment options for the negative effects of pes planus will increase. After the approval of the ethics committee, 32 participants who meet the inclusion criteria will be included in the study. The participants will be divided into two groups, 16 with pes planus and 16 without pes planus, by performing the navicular drop test. The foot muscle strength of the participants will be measured with a digital muscle strength device (Lafayette Manual Muscle). The International Physical Activity Questionnaire (IPAQ) will be used for the physical activity level of the participants, the 6-minute walking test for the exercise capacity, the SF-36 quality of life questionnaire for the quality of life, and the Foot Function Index (AFI) for the foot functional status. Heart rate, blood pressure, borg value and oxyhemoglobin value will be measured before and after the 6-minute walking test to be performed on the participants. According to these measurements, the physical activity levels, exercise capacities and quality of life of the participants in both groups will be compared.
BACKGROUND: Our study aimed to identify potential specific biomarkers for osteoarthritis (OA) and assess their relationship with immune infiltration.
METHODS: We utilized data from GSE117999, GSE51588, and GSE57218 as training sets, while GSE114007 served as a validation set, all obtained from the GEO database. First, weighted gene co-expression network analysis (WGCNA) and functional enrichment analysis were performed to identify hub modules and potential functions of genes. We subsequently screened for potential OA biomarkers within the differentially expressed genes (DEGs) of the hub module using machine learning methods. The diagnostic accuracy of the candidate genes was validated. Additionally, single gene analysis and ssGSEA was performed. Then, we explored the relationship between biomarkers and immune cells. Lastly, we employed RT-PCR to validate our results.
RESULTS: WGCNA results suggested that the blue module was the most associated with OA and was functionally associated with extracellular matrix (ECM)-related terms. Our analysis identified ALB, HTRA1, DPT, MXRA5, CILP, MPO, and PLAT as potential biomarkers. Notably, HTRA1, DPT, and MXRA5 consistently exhibited increased expression in OA across both training and validation cohorts, demonstrating robust diagnostic potential. The ssGSEA results revealed that abnormal infiltration of DCs, NK cells, Tfh, Th2, and Treg cells might contribute to OA progression. HTRA1, DPT, and MXRA5 showed significant correlation with immune cell infiltration. The RT-PCR results also confirmed these findings.
CONCLUSIONS: HTRA1, DPT, and MXRA5 are promising biomarkers for OA. Their overexpression strongly correlates with OA progression and immune cell infiltration.
The most frequently reported adverse reactions with OMNIPAQUE are headache, backache, neckache and stiffness, nausea, and vomiting. These reactions usually occur 1 to 10 hours after injection, and almost all occur within 24 hours. They usually last for a few hours, and usually disappear within 24 hours. Severe headaches persisting for days have been reported. Headache is often accompanied by nausea and vomiting and tends to be more frequent and persistent in patients not optimally hydrated.

Transient alterations in vital signs may occur and their significance must be assessed on an individual basis. Those reactions reported in clinical studies with OMNIPAQUE are listed below in decreasing order of occurrence, based on clinical studies of 1531 patients.

Headaches: The most frequently occurring adverse reaction following myelography has been headache, with an incidence of approximately 18%. Headache may be caused by either a direct effect of the contrast medium or by CSF leakage at the dural puncture site. However, in managing the patient , it is considered more important to minimize intracranial entry of contrast medium by postural management than attempting to control possible CSF leakage (see PATIENT MANAGEMENT).

Pain: Backache, neckache and stiffness, and neuralgia occurred following injection with an incidence of about 8%.

Nausea and Vomiting: Nausea was reported with an incidence of about 6%, and vomiting about 3% (see PATIENT MANAGEMENT). Maintaining normal hydration is very important. The use of phenothiazine antinauseants is not recommended. (See WARNINGS—General.) Reassurance to the patient that the nausea will clear usually is all that is required.

Dizziness: Transient dizziness was reported in about 2% of the patients.

Other Reactions: Other reactions occurring with an individual incidence of less than 0.1% included: feeling of heaviness, hypotension, hypertonia, sensation of heat, sweating, vertigo, loss of appetite, drowsiness, hypertension, photophobia, tinnitus, neuralgia, paresthesia, difficulty in micturition, and neurological changes.
PROBLEM TO BE SOLVED: To obtain a new deriv. which is absorbed well in oral administration by combining a sulfated oligosaccharide with a dibasic acid monoacylglycerin ester and to obtain a highly safe antiviral agent having high in-blood persistence therefrom. SOLUTION: A sulfated oligosaccharide deriv. having an increased oral absorbability is obtd. by substituting, with acyl groups represented by formula I or II (wherein R is a 1-18C alkyl or phenyl; A is a group represented by formula III or IV; (n) is 1-18; and (m) is 0 or 1), 5-80% of hydrogen atoms of saccharide hydroxyl groups of a sulfated oligosaccharide of which 20-95% saccharide hydroxyl groups have been sulfated. There is no limitation on the kinds of monosaccharide unit (glucose and galactose being easily available) constituting the oligosaccharide or on their arrangement, though laminaoligosaccharide and galactooligosaccharide are pref. in terms of the presistence of drug activity. Though there is no limitation on the site of acylation, the substitution of prim- or sec-hydroxyl groups of nonreduced terminal saccharides is pref.  This deriv. deactivates viruses without exhibiting anticoagulant activity and is highly safe as an antiviral agent.
Oral agents contain a cpd. the molecules of which (a) are sufficiently small to be able to penetrate any deposits on the teeth and (b) have sufficient functional gps. to be able to dislodge calcified deposits; the agents also contain a carrier. The pref. active ingredient is mellitic acid, which strongly inhibits the crystal growth of calcium hydroxyapatite and diminishes the dissolution and equilibrium solubility of calcium hydroxyapatite in acid. The agents inhibit the deposition of dental plaque due to pptn. of polyphosphates from the saliva, thus inhibiting dental caries.
Anatomic total shoulder arthroplasty is an established treatment for degenerative conditions involving the shoulder. One well-described complication of total shoulder arthroplasty is glenoid loosening, especially in younger and more active patients. As a result, several surgeons recommend against activities that may put patients at risk of implant failure after shoulder arthroplasty. Newer inlay and inset glenoid implants may have a role in treating arthritis in younger patients and may allow them to return to sports and high-impact activities, including weightlifting. These glenoid implants may also play a role in treating patients with notable glenoid deformity. However, more information is needed regarding their long-term outcomes.
Patient and family caregiver dyads will be recruited for a prospective cohort observation for at least 24 hours and a maximum of seven days. At inclusion, baseline and self-reported data from admission until inclusion will be collected. After inclusion, data will be collected every 24 hours on self-reported outcomes of the last 24 hours. Patients and family caregiver dyads will be followed until discharge, transfer to another department, death or a maximum of seven days of follow-up. If dyads stay longer than seven days, daily follow-up is ended and they are called once 14 days later to collect outcome data.
The Back-to-Back study consists of a cross-sectional study and a proof-of-concept study. The cross-sectional study aims to gain more insight into the peripheral underlying mechanisms of impaired proprioceptive postural control in patients with non-specific low back pain. Macroscopic, microscopic, hemodynamic, and electrophysiological characteristics of the lumbar multifidus and erector spinae muscles will be compared between patients with non-specific low back pain and healthy, age- and sex-matched controls. The interrelatedness between these back muscle characteristics and the correlation with proprioceptive postural control will be examined. The most discriminating muscle characteristics will be determined based upon which phenotypes of patients with non-specific low back pain will be delineated. The proof-of-concept study aims to assess the effects of high-load proprioceptive training on back muscle characteristics and proprioceptive postural control in patients with non-specific low back pain.
OBJECTIVE: Static assignment of participants in randomized clinical trials to placebo or ineffective treatment confers risk from continued seizures. An alternative trial design of time to exceed prerandomization monthly seizure count (T-PSC) has replicated the efficacy conclusions of traditionally designed trials, with shorter exposure to placebo and ineffective treatment. Trials aim to evaluate efficacy as well as safety and tolerability; therefore, we evaluated whether this T-PSC design also could replicate the trial's safety and tolerability conclusions.
METHODS: We retrospectively applied the T-PSC design to analyze treatment-emergent adverse events (TEAEs) from a blinded, placebo-controlled trial of perampanel for primary generalized tonic-clonic seizures (NCT01393743). The safety analysis set consisted of 81 and 82 participants randomized to perampanel and placebo arms, respectively. We evaluated the incidences of TEAEs, treatment-related TEAEs, serious TEAEs, and TEAEs of special interest that occurred before T-PSC relative to those observed during the full-length trial.
RESULTS: Of the 67 and 59 participants who experienced TEAEs in the perampanel and placebo arms during full-length trial, 66 (99%) and 54 (92%) participants experienced TEAEs with onset occurring before T-PSC, respectively. When limited to treatment-related TEAEs, 55 of 56 (98%) and 32 of 37 (86%) participants reported treatment-related TEAEs that occurred before T-PSC in the perampanel and placebo arms, respectively. There were more TEAEs after T-PSC with placebo as compared to perampanel (Fisher exact odds ratio = 8.6, p = .035), which resulted in overestimation of the difference in TEAE rate. There was a numerical reduction in serious TEAEs (3/13 occurred after T-PSC, one in placebo and two in perampanel).
SIGNIFICANCE: Almost all TEAEs occurred before T-PSC. More treatment-related TEAEs occurred after T-PSC for participants randomized to placebo than perampanel, which may be due to either a shorter T-PSC or delayed time to TEAE for placebo.