anand-s/m_qalm huggingface dataset:
Question: Which technique was used to determine the cellular locations of the CLU1 and CLU2 gene products?
A. intracellular and secreted
B. ER
C. intracellular localization
D. Golgi apparatus
E. immunofluorescence experiments
Answer: E
Question: What compartments inside the cell contain clusterin proteins?
A. ER and the Golgi apparatus
B. epitope tag
C. anibody
D. secretory pathway
E. secreted
Answer: A
Question: Which of the two CLU isoforms is the main one expressed in the choroid plexus?
A. fetal tissue
B. CLU1
C. clusterin
D. CLU2
E. cerebrospinal fluid
Answer: D
Question: Which clusterin single nucleotide polymorphism has been linked to a reduction in the risk for developing Alzheimer's disease?
A. CLU2
B. SNPs
C. rs11136000T
D. clusterin
E. CLU
Answer: C
Question: Which CLU protein isoforms in the brain have been characterized?
A. CLU1 and CLU2
B. clusterin
C. rs11136000
D. secreted proteins
E. AD
Answer: C
Question: Which CLU isoform has a consistently higher gene expression?
A. CLU2
B. ribosomal protein L13A
C. CLU1
D. allele
E. PNGase
Answer: A
Question: Which hormone can control the expression of CLU isoforms?
A. real-time PCR
B. cDNA
C. AD
D. rs11136000
E. androgen
Answer: E
Question: What effect do androgens have on CLU2 gene expression?
A. association
B. repression
C. inhibition
D. activation
E. expression
Answer: D
Question: Which particular histone deacetylase inhibitor has been shown to enhance the expression of CLU?
A. astrocytes
B. CLU2
C. therapeutic agents
D. valproate
E. amyloid
Answer: D
Question: How many residues does the CLU2 protein sequence have?
A. 449
B. protein
C. 82.3
D. 52.5
E. 6
Answer: A
Question: Which entity does allosterically regulate insulin degrading enzyme activity?
A. AD
B. Ab
C. somatostatin
D. microglia cells
E. IDE
Answer: C
Question: Which peptide hormone is the positive transcriptional regulator of IDE?
A. Ab
B. somatostatin
C. BV-2
D. AD
E. mRNA
Answer: B
Question: In which cell line was the gene expression regulation of IDE characterized?
A. mouse
B. astrocytes
C. microglia
D. BV-2
E. beta-amyloid
Answer: D
Question: Which method was applied to measure the quantity of IDE mRNA in the gene regulation experiments described in the paper?
A. ELISA
B. siRNA
C. RealTime PCR
D. Western Blotting
E. IgG antibody
Answer: C
Question: What regulates the production of neprilysin?
A. somatostatin
B. NEP
C. enzyme
D. matrix metalloproteinase
E. microglia
Answer: A
Question: What kind of glial cell is able to phagocyte b-amyloid?
A. neprilysin
B. siRNA
C. brain
D. culture medium
E. microglia
Answer: E
Question: What is the major protease produced by microglia responsible for degrading A?
A. Ab
B. cells
C. IDE
D. extracellular
E. beta-amyloid
Answer: D
Question: What substance exhibited a similar effect on  IDE secretion as achieved by somatostatin?
A. octreotide
B. analogue
C. endogenous modulator
D. substrate
E. beta-amyloid
Answer: A
Question: What are the sst receptors that are expressed on rat astrocytes?
A. SSTR-2, SSTR-3 and SSTR-4
B. SSTR-1, SSTR-2 and SSTR-4
C. somatostatin
D. microglia
E. rat
Answer: B
Question: What method was used to inhibit the expression of IDE?
A. Western blot
B. microglia
C. positive modulation
D. siRNA
E. culture medium
Answer: D
Question: What cell type in AD brains shows mitochondrial defects?
A. astrocytes
B. epithelial
C. fibroblasts
D. membrane
E. cytosol
Answer: A
Question: In which anatomical structure in the brain does amyloid-beta aggregate?
A. receptor
B. tissues
C. choroid plexus
D. fibroblasts
E. mitochondrial
Answer: C
Question: How many persons worldwide are estimated to have a medical condition related to neurodegeneration?
A. LPR2
B. mitochondria
C. 60%
D. more than 10 million
E. 70 years old
Answer: D
Question: Which protein is able to block nitric oxide creation?
A. amyloid
B. gelsolin
C. NO
D. mitochondrial proteins
E. cytotoxicity
Answer: B
Question: Which is the best-characterized factor that increases chances of getting AD?
A. damage
B. Swedish
C. pathogenesis
D. age
E. stress
Answer: D
Question: With which particular protein does amyloid-beta interact?
A. extracellular domain
B. disease-related proteins
C. mitochondria
D. receptor
E. gelsolin
Answer: E
Question: The aggregation of which peptide has been widely observed in brains of Alzheimer patients?
A. AD
B. amyloid-beta
C. extracellular
D. mtDMA
E. secretase
Answer: B
Question: What specific animal model can be used to study the role of amyloid-beta in apoptosis of choroid Plexus cells?
A. patients with AD
B. animal AD models
C. brain
D. APP/Ps mice
E. mouse
Answer: D
Question: Where does amyloid-beta assemble into oligomeric structures?
A. fractions
B. monomeric amyloid
C. membranes
D. synaptic terminals
E. lipid
Answer: D
Question: When does oxidative stress happen in AD patients?
A. transgenic mouse
B. predominantly in synaptic mitochondria
C. choroid plexus
D. before amyloid-beta accumulation
E. postmortem
Answer: D
Question: What effect can be observed when when γ-secretase is blocked?
A. APP-CTF accumulation
B. PSEN1 mutations
C. cell-based data
D. APH1 variants
E. transition-state analogue
Answer: A
Question: When APH1 genes are overexpressed in MEF KO what happens with the Aβ?
A. They are purified
B. They are anterior
C. They are shorter
D. They are longer
E. They are supported
Answer: D
Question: In which gene are mutations associated to many cases of early-onset familial forms of Alzheimer's disease?G
A. FAD
B. I-CLiPs
C. NCT
D. PSEN1
E. D257A
Answer: D
Question: What experimental technique was used specifically to purify the γ-secretase complex?
A. plasmids
B. affinity chromatography
C. lysate
D. cell lines
E. knockout experiments
Answer: B
Question: What peptide is able to control the expression of the ApoE gene?
A. AD
B. APP-CTFs
C. c-secretase
D. cholesterol
E. AICD
Answer: E
Question: Which amino acid is critical for the activity of the PS1 protein?
A. aspartate
B. C-terminal
C. 42-residue
D. 99
E. DDAA
Answer: A
Question: What experimental technique was used to determine the structure of γ-secretase?
A. densitometry
B. EM
C. ELISA
D. immunostaining
E. purification
Answer: B
Question: What candidate drug that blocks the γ-secretase is now tested in clinical trials?
A. LRP1
B. biochemical
C. PSEN1
D. AD
E. Semagacestat
Answer: E
Question: What mutation of the PS1 protein causes γ-secretase activity almost to disappear?
A. P436Q
B. L166P
C. wild-type
D. AICD
E. C100-His
Answer: A
Question: How many mutations relevant for familial forms of Alzheimer's disease have been detected for the PSEN1 gene?
A. 13
B. 42
C. P436Q
D. 185
E. PSEN2
Answer: D