MICROPHYSIOMETER

Abstract: The present invention is related to an array device for monitoring the effect of a physical or chemical stimulus on multiple small samples, said array device comprising a supporting substrate at least two array elements that are separated from each other by a isolation zone, said array elements comprising:• A receiving zone arranged to provide a contact between said one of said samples and said physical or chemical stimulus, said receiving zone having a cross-section smaller than 10 mm,• A heat detection means arranged to perform a measurement of heat between said receiving zone and a reference, and said isolation zone being formed by at least part of said supporting substrate characterised in that said supporting substrate has sufficient strength to support said array device and said isolation zone is arranged to thermally isolate said array elements.

Description: MICROPHYSIOMETER Field of the invention [0001] The present invention is related to a device and method for calorimetric measurements, especially to a hign throughput screening device for detecting energy changes . State of the art [0002] Calorimetry m general is a measurement principle that detects all processes that occur m a reaction vessel. Calorimetry has several advantages: calorimetry is the most general detection principle, since most processes, physical, chemical or biological are accompanied by changes m heat content. This can be useful m the analysis of very complex processes, because :: is more likely that: unκnown phenomena will be discovered. Moreover, the change of temperature of a reaction volume is dependent upon the concentration of the reagents, not upon the absolute quantity, offering the possibility of miniaturisation. Additionally, calorimetric methods are not dependent upon the sample form (the sample can be solid, liquid, gaseous, or any combination thereof, and neither colour, optical transparency, nor absence of suspended matter are requirements) . Calorimetric measurements are non- invasive (it is not necessary to disturb the biological system, for example, by radiation) . It even allows on-line monitoring of living cells over a longer period of time to obtain kinetic data. It is non-destructive towards the sample (no need for fluorescent markers or fixation procedures; . The stare of the art are devices capable of measuring small temperature differences between relatively large reaction volumes (typically Z .12 ml to 100 ml). SETARAM (Caluire, France) is a provider of calorimetric devices. These products are relatively large batch systems, mainly intended for applications in the biochemical field. The ITC ® (Isothermal Titration Calorimeter, croCal, LLC, Northampton, USA) has a cell volume of 1.3 ml (and a detection limit cf 400 nW) . The TAM ® (Thermal Activity Monitor, Thermometric , Jar alla, Sweden) has a cell volume of 4 ml (and a detection limit of approximately 100 nW) . These micro-calorimeter batch systems are non-compatible with high- throughput requirements due to the relatively large volumes, the apparatus' closed structure and long cycle times (typical cycle times are 2 to 3 hours) . [0003] Commercially available micro-calorimetric sensors have also been described, using thermopiles and thermistors (Xensor Integration, Delft, The Netherlands). A main disadvantage of these devices is that they are configured in such a way that the reference temperature is the temperature of the silicon border. This leads to problems concerning baseline stability and common rejection mode since no differential measurements are used. Additionally, these devices are net compatible with standard robotics used in high throughput screening since no receiving zones are integrated. [0004] The Cell Monitoring system (CMS " ) developed by B . Wolf (University of Rostock, Germany) is a recently developed multiparametric chip including a pH microelectrode, oxygen and temperature sensor, reference electrode, a transparent aperture and interdigitated electrodes (IDES) to detect cellular metabolism. It is a modular system m whicn different sensors are "glued" together, resulting in a relatively large measurement chamber volume. This can cause problems with regard to quantification. [0005] Physiometers are instruments designed to measure the activity of living cells and are sometimes referred to as whole-cell biosensors. They are mainly used for investigating cellular components, like ion channels m the cell membrane and for determining the effect of chemicals, physical stimuli and radiation on the activity of cells or tissue. Besides their usefulness m the field of pharmaceutical research, toxicology, oncology, and environmental studies they also have important potential applications m industry as a screening tool m the development of drugs and agrochemicals . [0006] US Patent No. 6046056 describes a method of screening a plurality of test compounds for an effect on a biological system. Microfabπcated substrates having one substrate and at least two intersecting channels fabricated m this substrate are used. The intersecting channels have a cross-sectional dimension from 0.1 μ to 500μ. The device further contains a source of test compound fluidly connected to a first channel and a source of at least one component of the biochemical system fluidly connected to the second channel. The apparatus further comprises a detection zone m the second channel . [0007] US Patent No. 5104804 relates to a microflow- chamber which makes use of a porous microchamber . At least part of the chamber wall is constructed from a porous material so that fluids can enter m the microchamber and cells cannot escape. Tne microchamber serves as disposable device for placing cells in the microflow- chamber so that the properties of the cells can be measured. [0008] The industrial physiometers known in the art however make use of optical detection principles, usually a CCD camera to detect fluorescence or chemiluminescence m the reaction vessel . [0009] International Patent Application W09745733 discloses a device and method for performing high- throughput screening of physiological response of cells to biologically active compounds. The method is based cr. multicolour luminescence reading. In this method, the cells contain at least one luminescent reporter molecule. Fluorescence or chemiluminescence requires labelling of molecules with the disadvantages described above. Moreover, only 50 % of the activity changes can be visualised by this method, whereby information on cell signalling can be lost . Furthermore, fluorescent and chemiluminescent probes can be hazardous for the environment . [0010] EP-A-0921391 discloses a device capable of performing a differential heat measurement between a sample and a reference reaction. The device uses a thermopile tc measure the differences in heat release between twc receiving zones. However, this device is not compatible with the standard research formats and cannot be miniaturised while maintaining sufficient isolation between the measuring receiving zones, leading to false results and poor precision. Aims of the invention [0011] The present invention aims to provide a new microphysiometer device, which can be used in a high- throughput screening procedure which can measure a broad scope of chemical and physical interactions. Summary of the invention [0012] The present invention comprises an array- device for monitoring the effect of a physical cr chemical stimulus on multiple small samples, said array device comprising on a supporting substrate at least two array elements that are separated from each other by a isolation zone, said array elements comprising: • A receiving zone arranged to provide a contact between said one of said samples and said physical or chemical stimulus, said receiving zone having a cross-section smaller than 10 mm, • A heat detection means arranged to perform a measurement of heat between said receiving zone and a reference, and said isolation zone being formed by at least part of said supporting substrate characterised m that said supporting substrate has sufficient strength to support said array device and said isolation zone is arranged to thermally isolate said array elements . [0013] Said reference can be an external reference, or a neighbouring receiving zone. [0014] In the case a neighbouring receiving zone is used as reference, said heat detection means is preferably a differential heat detection means arranged to measure differences m heat between both receiving zones. [0015] The array device of the present invention is preferably dimensioned as a standard 96, 384, 1536 or 6144- well microtiterplate . [0016] The array device of the present invention can comprise resistors m said receiving zones. This set-up allows for providing thermosettmg and/or thermal calibration means for every receiving zone m the array device . [0017] In the array of the present invention, said receiving zones can comprise a bottor surface and sidewalls. Preferably, said bottom surface comprises said supporting substrate. In a specific emoodiment of the present invention, said bottom surface is perforated. [0018] Further, m the array of the present invention, said isolation zone preferably comprises a bottom surface, formed by said supporting substrate, and an isolator comprised between the receiving zones. Said isolator is preferably air. [0019] In tne array of the present invention, said differential heat detection means is preferably selected from the group consisting of thermopile, tnermistor, diode, IR detection means and CCD camera. [0020] The receiving zones according to the present invention can further comprise a detection means selected from the group consisting of a light addressable potentiometric sensor, a pair of mterdigitated electrodes, a field effect transistor, a diode, a reference electrode, and a working electrode. [0021] Possibly, the array of the present invention comprises receiving zones which are chemically modified to physically contain said sample and said chemical or physical stimulus within said receiving zone. [0022] Advantageously, the receiving zones of the array according to the present invention have a cross- section of between 10 μm and 9 mm. [0023] Another aspect of the present invention is a calorimetric measuring method for measuring multiple samples, said method comprising the steps of: • Providing an array device according to the invention, • Providing said samples m said receiving zones, • Providing a chemical or physical stimulus tc said samples and, • Measuring the heat released by said samples. [0024] A further aspect of the present invention is the use of tne device of the present invention for enzyme discovery. [0025] A further aspect of the present invention is the use of tne device of the present invention for drug identifica ion . [0026] Another aspect of the present invention is the use of the device of the present invention for agrochemical identification. Short description of the drawings [0027] Figure 1 a and b are illustrations cf the device according to the present invention. [0028] Figure 2 shows two neighbouring receiving zones on an array device according to the present invention . [0029] Figure 3 depicts a perforated membrane or sieve . [0030] Figure 4 draws a suspended membrane. [0031] Figure 5 is an illustration of supply means based on capillary forces. [0032] Figure 6 a to c shows a preferred embodiment of the present invention. [0033] Figure 7 describes an api-Zym chamber: each well refers to a specific enzyme. The color change is measured optically. Detailed description of the invention [0034] The present invention discloses a device for monitoring tne effect of a physical or chemical stimulus on a sample by measuring the heat generated. The sample can be biological material or non-bioicgical material, depending on the application, as described below. Preferably, according tot the present invention, a plurality of devices are set up m an array, contained m an apparatus. The device comprises a substrate. This substrate comprises at least a first and a second surface, and at least two substantially identical receiving zones or reaction vessels which form a part of the substrate. The receiving zone is used for retaining the sample and for providing contact between the sample and the physical or chemical stimulus. The device further comprises at least one heat detection means, preferably integrated m the substrate, for monitoring changes in heat content or enthalpy generated by the sample upon being contacted by a stimulus. Said heat detection means is arranged to perform a measurement of heat between said receiving zone and a reference. Said reference can be an external reference or a neighbouring receiving zone. A heat detection means can be selected from the group consisting of thermopile, thermistor, diode, IR detection means and CCD camera. A di ferential heat detection means can be operatively associated with a first and a second receiving zone so that a differential measurement between the first receiving zone and the second receiving zone can be performed. [0035] Preferably, the differential heat detection means is positioned in such a way that the distance between the differential heat detection means and the first receiving zone is substantially identical to the distance between the differential heat detection means and the second receiving zone. The isolation zone surrounding the first receiving zone and the isolation zone surrounding the second receiving zone of the same device are preferably in substantially thermal equilibrium. [0036] The differential heat detection means performs a differential measurement of heat or enthalpy also called calorimetric measurement) between the two receiving zones. A device comprising such differential heat detection means is also referred to as twin detectors. Twin detectors have two identical receiving zones, one for monitoring a reference reaction and the other for monitoring the test reaction. The reference reaction is selected to resemble the test reaction as closely as possible. The signal is thus purely differential and disturbances like condensation or evaporation are cancelled out. [0037] Techniques used to fabricate this array can be, but are not limited to, molding or micro-electronic processing. Micro-electronic processing offers specific advantages such as cost reduction if mass production is envisioned. Further, extreme miniaturisation is possible, the degree of which is limited by e.g. lithograpny. A high degree of parallelism is possible, making high- hroughput feasible. Moreover, the detection limit of the system can be decreased considerably because fluidics are integrated on the same chip as the sensing element, reducing the length of the thermal path from reaction to sensing site In addition, the detection limit of the system can be decreased even more if pre-ampliflers are integrated on the chip. Array An "array" as used herein refers to a set-up cf at least two devices, which are substantially identical. The distance between neighbouring receiving zones cf each of the at least two devices is substantially identical. Said array has preferably the format of a standard microtitre plate, such as, but not limited to a 96-well, 38^-well, or 1536-well microtitre plate (see table 1) . Table 1 . Illuε tra zi on of ti tre plate formats and use . [0038] Alternatively, the design of the array can be customised for integration m any high-throughput screening system. This allows the use of the array m standard robotics used m e.g. drug screening. In a preferred embodiment of tne invention, the array is a sensor-arrayed chip with the footprint of a standard 96-well titre plate, and thus compatible with pharmaceutical robotics for dispensing and titre plate handling. The distance between adjacent wells m this format is 9 mm. For formats derived from this reference, the inter-well distance is 9 mm divided by the miniaturisation factor. The miniaturisation factor is defined as udls m - 96 with n_wells the number of wells. According to the present invention, the array is made with techniques allowing the formation of very small receiving zones compatible with standard arrays used. Sample and Stimulus [0039] The "sample" as referred to m the context of the present invention can be biological material or non- biological material. Biological material can be, but is not limited to, a bio-molecule, any biological cell or cell- culture (m monoiayer or suspension) , a tissue (natural or artificially generated) , an organ, part of an organ, a micro-organism or a group of micro-organisms , one cr more small animal, one or more plants More specifically the biological material can be genetically modified to display specific characteristics, such as, but not limited to, the expression of a molecule such as, for instance a receptor or ion channel. Thus, according to a preferred embodiment of the invention, the first or reference receiving zone of the device can comprise an unmodified cell or culture thereof, while the second receiving zone comprises the cell or a culture of these cells which nave been modified to display a specific characteristic. By contacting the cells of both receiving zones with a sti m ulus it is possiole to monitor the effect of the presence of this specific characteristic on the reaction of tne cell to the stimulus. [0040] The biological material cr sample can be applied directly or can be applied m a medium. Medium can be understood as any material or substance suitable for containing biological material, preferably keeping said biological material m its physiological state. Thus medium can refer to substances such as but not limited to cell culture media, physiological buffers, etc. [0041] Non-biological material can be, but is not limited hereto, polymers, catalysts, ... [0042] A stimulus as used herein refers to a chemical, biological or physical signal that is applied to the sample m order to determine its effect on the sample. [0043] It will be understood that the nature cf the sample and the stimulus m the present invention will be determined by the purpose for whicn the apparatus cf the present invention is used. Additionally, the present invention, by providing the possibility to measure extremely small differences m temperature m extremely small reaction volumes, m circumstances which are compatible with biological cells, allows the use of calorimetric measurements for interactions that previously could only be detected by other, more invasive methods. A more detailed review of the applications and corresponding samples and stimuli associated therewith is provided in the examples herein. [0044] In a preferred embodiment of the invention the apparatus is applied m high-throughput drug screening, whereby the sample can be a biological cell, such as, but not limited to, a human or animal cell m culture, including a cancer cell, and the stimulus comprises a library of drug candidates. [0045] It will be understood that, m the context of the present invention, it can be envisaged that the sample and the stimulus are interchangeable. For instance, if ooth sample and stimulus are chemical molecules, it is clear that the choice of sample and stimulus will depend on the design of the measurement. Differential heat detection means [0046] For the purpose of this invention, the interaction between the sample and the physical or che~ιcal stimulus can be called "event" . [0047] In case an event is created, different signals can be obtained such as change of heat or enthalpy, change of ionic concentrations of different ions, etc... [0048] The differential heat detection means (DHDM) of the present invention is m contact with the two receiving zones at a first and second detection zone. For most applications one of the receiving zones will be used as a reference while m the other an event is generated. When a change of heat or enthalpy is generated, this signal is measured by the DHDM, whereby the thermal input signal is converted into a differential electrical output signal. For instance, to test the effect of a chemical en a biological cell, botr the first (reference) and second receiving zone will contain the biological cells. The reference sample will ne contacted with a placebo solution, while the other is contacted with the chemical. Alternatively, such as m e.g. drug screening applications, reference cells are present m a first receiving zone and genetically modified cells are present m a second receiving zone When e.g. a chemical stimulus is added to both receiving zones, a difference m effect on the genetically modified cells will cause a heat change at one side of the differential heat detection means, thereby producing a differential voltage Preferably, the reference sample is thus substantially equivalent to the test sample, so that heat capacities and surface relationships do not influence the measurement . [0049] The device as such has a very high common mode rejection ratio, offering a signal which originates from the chemical cr physical stimulus stimulating or suppressing the metabolism of the cells under study. The isolation between tne first receiving zone and second receiving zone should oe high, so that the processes m both receiving zones oo not influence each other, e.g. the temperature m one receiving zone is preferably not influenced by the temperature m the second receiving zone. This is accomplished by ensuring an isolation zone around the receiving zones (see "receiving zone" below) and around each device. The thermal isolation of the isolation zone between two neighbouring devices ( "mter-device isolation zone") is at least equivalent to that of the isolation zone between the two detection zones within a device. Preferably, the thermal isolation between the devices is higher than the thermal isolation between each of the receiving zones of the same device. [0050] According to a preferred embodiment of the invention, the differential heat detection means is a thermopile, consisting of a set of 2 temperature sensitive means with a differential read out. Said thermopile has a hot and a cold junction, which are operatively associated with the first and the second receiving zone respectively. Said hot and cold junction are thermally isolated one from another . [0051] A thermopile is a sensor that inherently makes use of the twin arrangement principle A thermopile is made up of a number of thermocouples, electrically connected m series, thermally connected m parallel. [0052] The mam advantages of a thermopile are: • The thermopile is a self -generating offset-less device, as the heat flowing through it supplies the power for the output signal . As a result there is no offset drift and no interference caused by power supplies. • The sensitivity of the thermopile is hardly influenced by variations m the electrical parameters across the wafer or by the temperature. [0053] The thermopile can be optimised m terms of dimension and number of thermo-electric strips. The optimum is found analytically, with the help of MATLAB . The other sensing principles and supporting structures (e.g. the resistors m the capillary walls) are designed to fit m this optimised thermopile chip architecture Substra e [0054] The substrate as used m the context of the present invention can be any material that allows the fabrication of the array device m the dimensions of a microtiterplate . The substrate can be, but is not limited to, one of the group consisting of silicon, silicon oxide, silicon nitride, silicon oxynitπde, polysilicon, porous silicon, plastic, polymer (also rubber, PVC, etc..) , biodegradable polymer, glass, quartz, ceramics, aluminium oxide, agar, biological material, and rubber. When the substrate is a material compatible with semiconducting processing, the integration of sensors and fluidics on the same chip is facilitated. This opens the way to small sample volumes and inert reaction vessels, since no reaction with materials or adsorotion of materials occurs. Receiving zone [0055] For the purpose of this invention, the term receiving zone or reaction vessel is used to refer to a means or carrier of the sample. More specifically, if the sample used is biological material, such as a cell culture, the receiving zone will preferably be of a format capable of containing fluids. Preferably, said receiving zone is capable of handling volumes m the range from 0 ml to 100 ml, more preferably from 0 ml to 7 ml, especially preferably from 0 ml to 5 ml , most preferably from 0.1 nl to 1 ml. Preferably, said receiving zone has a maximum volume of 5 ml . [0056] The receiving zone should operatively be associated with the differential heat detection means. This means that there is a thermal coupling between the receiving zone and the temperature sensitive part of the differential heat detection means. This can be, but is not limited to, the hot or cold junction of the thermopile. [0057] According to a preferred embodiment of the invention, the receiving zone is formed by a recess (10) m a substrate (see figure 6a-c) ) . The recess can have any 3- dimensional shapes and can be, but is not limited to, cylindrical shaped, cubic shaped, bar shaped, truncated pyramidal shaped, truncated conic shaped. Said recess can extend from the first surface of the substrate to the second surface or can extend from the first surface tc the bulk substrate material . Said receiving zone can also re an area on the substrate (11) e.g., the whole substrate or parts of the area of the suoεcrate (11) can be chemically or physically modified m such a way that they can selectively hold one or more cf said sample, medium, and chemical stimulus. [0058] Alternatively, the receiving zone can be a microvessel made of a material, such as but not limited to, metal, steel, silicon, silicon oxide, silicon nitride, silicon oxynitπde, polysil ccn, porous silicon, plastic, polymer (also rubber, PVC , etc.), biodegradable polymer, glass, quartz, ceramics, aluminium oxide, agar, biological material, and rubber (12), which is either placed on top of, or is hanging above the substrate. For instance, the receiving zone can be formed by a needle form dispenser. Preferably, the distance between the substrate and the receiving zone is not larger than the distance between two adjacent receiving zones In Figure 6a-c, the differential heat detection means is referred to as 13 , the substrate is referred to as 14. [0059] Said receiving zone is surrounded by an "isolation zone" , which functions as a thermal isolation zone. Membrane [0060] A membrane as used herein refers to a part of the device which provides thermal isolation. The membrane can be part of the substrate or can be a second substrate. When the receiving zones are formed by a recess m said substrate, a membrane can cover the recess at either the first surface or at the second surface of the substrate. When the recess extends from the first surface of the substrate to the bulk material, and the substrate from the bottom of the recess (the wall of the recess being substantially parallel with the first or the second surface of the substrate) to tne second surface forms an insulating layer, this part of tne substrate is considered to be the membrane . [0061] When the receiving zone is formed oy complete etches through the substrate extending fror the first surface to the second surface, a thin membrane can be formed for covering said recess at a first side or at the second side of the substrate. [0062] The memnrane can be made of tne substrate material or of another material . The membrane can be formed, by methods such as, but not limited to, growing a membrane layer on the substrate cr bonding a mettorane . [0063] The memcrane can also be made cf another material than the substrate material. For instance, a thin layer of the membrane material can be formed on the substrate material . The recess can be formed m the substrate by conventional micromachinmg tecnniques . The recess can extend from the first surface of tne substrate until the membrane. When e.g. dry etching tecnniques are used, the membrane material and etch chemistry can be chosen such that a recess is etched m the su strate and that the etching process selectively stops on the membrane. The thickness of the membrane can be between 1 μm and 1 cm, preferably between lμm and 1 mm, most preferably oetween 10 μm and 0.1 mm. The thickness depends on the membrane material, and consequently on the thermal isolation of the membrane material. For example, the thickness of a silicon oxide membrane can be lower than 10 μm. For glass, the thickness can be between 1 and 5 mm. [0064] Any of the above mentioned materials can be perforated and/cr patterned material such that a perforated membrane cr sieve is created. When a liquid is supplied to the receiving zone, oxygen molecules can escape through these openings. Additionally or alternatively, the sieve serves to encapsulate biological material . Sieves are useful when specific elements, like cells or tissue, must be entrapped inside the physiometer, while maintaining (periodic) contact with a nutritive medium. [0065] The sieve's square openings are made m a membrane m sucr. a way that that their width is tne same as the width of tne surrounding bar. The size of the openings is determined by the function of the sieve. For instance, when desiring cellular entrapment, the openings will preferably have a diameter of about 6 μm, while for tissue entrapment the openings will preferably have a diameter of about 20μm. It is understood that the size of the openings can be optimised depending on the sample used. [0066] The sieve is preferably made of low stress material (1.2 μm plasma oxide) . In places where sensors are positioned, other structural layers are present. [0067] When the receiving zone is a recess m the substrate, the openings will extend from the bottom of the recess to the second surface of the substrate . When the receiving zone is part of the first surface, the openings will extend from this first surface to the second surface. Said membrane can also be a suspended membrane (see figure 4) . [0068] The device or array of devices can further comprise a membrane which covers it. This membrane can also be called "lid" . The lid can be made of at least one material such as, but not limited to, metal, steel, silicon, silicon oxide, silicon nitride, silicon oxynitπde, polysilicon, porous silicon, plastic, polymer (also ruboer, PVC, etc..) , biodegradable polymer, glass, quartz, ceramics, aluminium cxide, agar, biological material, and rubber. [0069] The same sieve structure as described for the membrane can be found m the lid. The lid can cover the receiving zone, as shown m figure 3. In this way, cell or tissue can be entrapped. Extra detection means [0070] According to one emoociment of the invention, the device can further comprise an extra detection means. Said extra detection means can ce a light addressable potentiometπc sensor (such as, but not limited to, a Light Adressable Potentiometπc sensor or "LAPS"), a FET device, a diode, (mterdigitated, "IDES"; electrodes, a reference electrode, a working electrode and/or an impedance spectroscopic device. Said extra detection means can be placed such that it is operatively associated with said receiving zone. For example, said extra detection means can be integrated m the substrate. Wnen the receiving zone is a recess m the substrate, the extra detection means can be integrated m e.g., the side walls or the bottom wall of the recess When the receiving zone is an area on the substrate, the extra detection means is preferably integrated m said substrate. The number of extra detection means per receiving zone is not limited. Calibration means [0071] In the context of the present invention it is envisaged that, m particular circumstances or for certain applications it may be required to set the device at a given temperature. Therefore, the device may further comprise a calibration means for thermosettmg said device. Tne calibration means can be a tuneable electrical power generating means such as e.g. a resistor or a tnermop_le or a temperature sensitive means such as a resistor, diode, thermopile or a microprocessor that drives the power generator m such a way that a pre-determmed temperature is obtained. ■Supply means [0072] Additionally, the device of the invention can comprise a supply means to fill the receiving zones an active (e.g. pressure or suction force) or passive (e.g. capillary force) manner. Said means can ne fabricated m a material such as, but not limited to silicon, silicon oxide, silicon nitride, silicon oxynitride, polysilicon, porous silicon, plastic, polymer (also rubber, PVC, etc..) , biodegradable polymer, glass, quartz, ceramics, aluminium oxide, agar, biological material, and rubber. In a preferred embodiment of the invention, the supply means has the same dimensions and layout as the device, except the differential heat detection means is omitted. The supply means can optionally have external or internal (e.g. made by micromachmmg) pumps and valves. Alternatively, said supply means can also be an industrial dispenser with or without external pumps and valves. The supply means can also be a custom-made dispenser with or without external or internal (e.g. made by micromachmmg) pumps and valves. [0073] In the preferred embodiment, the array ma es use of capillary forces to supply the solutions to the receiving zone(s) . Figure 4 illustrates one way to accomplish this. The sample solution only fills those receiving zones which are either open-ended or sieve-ended, because the air must be able to escape. A hydrophilic native silicon oxide covers the inner walls of tne capillaries. To avoid wetting of the whole chip via the membrane, hydrophobic polyimide rings are deposited on the bottom of the rims of tne capillary. [0074] The height h of the fluid column inside the a capillary is: h = cos Φ fW with γ the surface tension of the analyte solution (72 mN/m 2 for water , p its density, g gravitational acceleration, r the equivalent hydraulic radius, and φ the contact angle betweer the analyte and the capillary If water is applied tne capillary of the 384-well micro titre plate (m=2) tne neight equals 4.1 mm ( ° ) , 6 8 mm (0=35°) . The water column will thus fill the whole capillary. If different solutions need to be tested on the chip, commercial dispensing tools can be used up to the m=4 case. For higher density chips, piezo-dπven dispensers are needed. [0075] In hign-throughput drug screening, the potential drug candidate is diluted m water or dimethyl sulfoxide (DMSO) . One possible way to accomplish this is illustrated m Figure 5. In a first phase, the chip is filled with water or DMSO, by using capillary forces. In a second phase, a dispensing tool supplies the drug candidate. Diffusion processes (passive) accomplish mixing [0076] The emptying is accomplished by contacting the meniscus formed due to gravity, with a hydrophilic surface. Washing of tne capillaries can be accomplished by repeating the passive filling and emptying several times. [0077] The device of the present invention can further comprise read-out electronics such as (pre-) amplifying, multiplexing, filtering and/or buffering circuitry. [0078] For calibrating said the calorimetric measurement, resistors, other than those used for thermosettmg, are used as calibration means and are situated m the walls of the recesses (1 recess - 1 calibration resistor) . The calibration resistor is designed to be able to produce dissipation heat the same range as the produced biological power. Its value is preferably 800 k . Examples ; Example 1: Development of a microphysiometer comprising a thermopile as differential detection means. [0079] A microphysiometer was developed, which comprises a device which allows the detection of extremely small temperature differences between two reaction vials, which is biocompatible and of which an array can be formatted which has the footprint of a microtitreplate . In order to correspond to a 96-well plate format, the distance between the centre of the two substantially identical receiving zones of the device was designed to be 9 mm. [0080] As substrate material for the device silicon was selected. [0081] The herein described device was developed for processing on an 8 mm membranous chip. However, it will be understood that, the use of other sunstrate material may dictate adjustments m certain described settings. [0082] The use of silicon as substrate material provides the possibility of integrating the sensors and fluidics on the same chip, so that sample volumes can be m imalized (minimal dead volume) . Additionally, by forming the receiving zones as recesses m the substrate, inert receiving zones are obtained, as no reaction or sorption of materials occurs. Because of its good thermal conductivity, making thermal sensors of silicon requires specific measures The bulk material short circuits the thermal signals, and should be removed by post-processing. [0083] As differential heat detection means a thermopile was integrated m the substrate. Additionally, 2 resistors were integrated m the walls of the recesses forming tne receiving zones as thermosettmg means. [0084] As described above, the device comprises, besides the thermopile, two receiving zones and two resistors. Eacn of the receiving zones is surrounded by an isolation zone [0085] The receiving zones are formed by providing a recess a substrate and can be seen as vertical capillaries extending from the first surface of tne substrate to the second surface of the substrate. Thus, tne receiving zone is a hollow silicon volume, m substantially upright position respect to the wafer plane . They nave square cross-sections and they are dry-etched m the silicon substrate. A thin layer of a membrane material (different from the substrate material, substrate=sιlιcon; membrane=sιlιcon dioxide) is formed on the second substrate or membrane material, the etching process will stop on tne silicon oxide. Due to the dry etching process, the corners of the square are rounded. The height of the recesses is substantially the substrate thickness. In case of a 6 inch wafer, this is 625 μm, in case of an 8-ιncn wafer, 725 μm. If necessary, the substrate can be made thinner. [0086] The use of vertical capillaries has many advantages over droplet dispensing: d) It increases the volume to contacting surface ratio of the analyte solution to the chip. This results a more compact chip for the same sample volumes, (n) it decreases the evaporation rate. The free contact surface of the sample is only at both open edges of the receiving zone. [0087] The membrane was perforated to obtain a sιe ιr e of which the openings were chosen to be 6 μm wide, to enable cellular entrapmen . [0088] Each of the receiving zones of the device was provided with a thermal isolation zone which corresponds to a recess the substrate. Each receiving zone is bordered by a double silicon rim, which encloses the thermal isolation zone so that the inner rim is common between the thermal isolation zone and the receiving zone. The width cf the rims must be minimal to maximise the thermal isolation between two neighbouring receiving zones. The minimal dimensions are set by: (l) mechanical stability issues: m order to give decent support to the membranous chip, the minimal rim width between two neighbouring devices, is set to be lOOμm, meaning 50μm for each receiving zone + accompanying isolation zone, (n) (n) technological issues: dry etching technology easily allows for etch ratios of 15. For processing on an 8 -inch wafer, with a thickness of 725μm, a rim width of 50 μm, which is feasible and not critical, was chosen. [0089] A device comprises two receiving zones and two isolation zone. The units of 1 receiving zone and one isolation zone are mirrored around an Y-axis. [0090] The devices can also comprise a thermosettmg means. Two resistors are integrated m the walls (surrounding rims) of each receiving zone, one of which is the calibration resistor, is the other the thermosettmg resistor. The latter is designed to be able to produce dissipation heat to heat up the receiving zone to a temperature m the range of room temperature to 100°C 1 mm when filled with water. Its value is 130 k . [0091] Reference and working electrodes are present in each recess. Example 2: development of different arrays of the devices [0092] The device as developed in Example 1 was designed for a sensor-arrayed chip with the footprint cf a standard 96-well titre plate, to be compatible with pharmaceutical robotics for dispensing and titre plate handling (the 96- well format of microtitre plates being considered as the reference for the moment) . The distance between adjacent wells m this format is 9mm. For formats derived from this reference, the inter-well distance is 9mm divided by the miniaturisation factor. The miniaturisation factor is defined as: i n w ls m = . 96 with n_wells the number of wells. The format used in the present example is a 1536-well (m=4), with 8* (8*12) devices on the substrate . [0093] A 3-D view of a part of the array is shown m figure la. A thermopile connects each two neighbouring cells . Example 3 : Applications of the microphysiometer of the present invention. [0094] The device of the present invention makes it possible to measure the interaction between two molecules in a very sensitive, physiologically relevant way, while its scale allows integration into industrial robotics, opening enormous possibilities for its use as a high- throughput screening tool. A. Industrial enzyme discovery [0095] According to a preferred embodiment of the invention tne device is used for screening enzyme activity m the identification of new enzymes. [0096] The field of industrial enzyme discovery is now profiting from the genomics ano assay technology revolution. Analysis of gene expression libraries from various organisms allows the discovery of many enzymes, or biocatalysts , wmch brings new solutions to industry The use of enzymes to effect chemical transformations, also called biocataiysis , has grown enormously over the last 15 years, and is now a major contributor to industrial synthesis of fine chemicals and pharmaceutical precursors. [0097] Traditionally, cultures of different bacteria are set up and cell extracts are prepared. These are then tested for active components, which are purified. The corresponding DNA sequences is isolated and expressed m a domesticated host m the hope the same active component can be obtained. The use of gene expression libraries has brought significant advantages: it avoids having to keep varieties cf bacteria cultures, many of which require specific conditions (i.e. thermophyles) ; and it allows rapid identification of the active molecules and the DNA sequences encoding them. Furthermore, expression can be directly tested m the desired domesticated host. However, the bottleneck m the identification of new biocatalysts remains the screening of the cell extracts and expression libraries for the production of enzymatic activity on the target of interest. Often a high number of extracts or expression products are generated and a method is needed to allow sensitive and rapid identification of the clones/extracts that produce/contain molecules that are active on tne target, and this on a large scale. The following describes how such a screening can be performed with the device of the present in d ention. 1. Preparation of the sample a) bacterial cell culture: a collection of different bacteria strain is grown at respective optimal temperatures; a cell extract cf each strain is prepared by disrupting bacteria with either ultrasounds or a lysis buffer . All extracts are normalized at 1 mg/ml and are distributed 96 or 314 well m a registered order. b) an enzyme library can be purchased from suppliers or prepared from bacteria containing a plasmid from which an enzyme can be expressed. Such liorary can be prepared m a microplate format. The enzyme concentration is then normalized to a specific concentration which is to be determined m each case. The libraries can contain many unique enzyme variants with yet unidentified properties. The different enzymes can be tested for the improvement of various industrial processes. Plates can be stored at -80°C or -20°C for several weeks. 2. Screening operation [0098] If an enzyme that modifies target A (i.e. A is the "substrate" m chemical terms) is to be found, target A is prepared m a screening buffer and constitutes the sample . - A number of reference receiving zones (blanks) are loaded with screening buffer, while the reaction receiving zones are loaded with target A screening buffer. The temperature is monitored real time. The reaction receiving zones are then loaded witn tne extracts or cell supernatants (or (partiall * pur_fιed fractions thereof) containing the enzyme. As a control, an enzyme known to act on target A is added m a number cf reaction receiving zones (instead of the extract cr supernatant) . Preferably the solution of control enzyme is m all aspects (except for the presence of the control enzyme) similar to the extract or cell supernatant solutions tested. Temperature is monitored m real time. [0099] If the extracts or supernatants contain enzymatic activity which act on the target the sample, tne reaction enthalpy will heat up the reaction receiving zone and this will be detected by the DPDM. Additionally, if a pH detector is also included m the device, the possible accompanying change proton concentration could be detected by means of the pH detector. Enzyme potency is function of the quantification of temperature change m time. Any cell extract that generates heat relative to the control wells is considered a hit. The well coordinates will allow the identification of the corresponding bacteria strain or clone. [0100] If the organisms are "extremophyles" which grow at very high temperatures (70 to 100°C or aoove) , the enzymatic activity can still be monitored at such temperatures using the thermosettmg unit . 3. Processing of the hits [0101] In the case of the screening of bacterial extracts, the "hit" extract can be further fractionated to determine, optionally, with the same device, the suo- fraction localization of the enzyme. Such fractions containing the enriched enzyme can then be used as biocatalyst preparation for industrial purposes. [0102] The expression genome of the studied bacterial strain can then be prepared as a cDNA library and further subcloned m an expression vector. The library can t en be introduced m a suitable host (Phage, E. coll, B. suotilis, etc..) and induced. Clones containing a single copy cf each gene can then be isolated and grown m a microplate format, while keeping a reference microplate for each screening plate. Cell extracts can then be prepared and screened for the desired activity as described earlier. A hit well will then correspond to a clone m the reference microplate. The plasmid containing the desired gene can then be amplified, sequenced and isolated for further processes such as bulk enzyme production Such preparations can be used for industrial purposes . [0103] In the case of direct screening of cDNA libraries the "hit" can be directly identified as a desired compound. The enzyme can be identified and purchased or prepared using the corresponding "hit" clone to obtain quantities suitable for industrial processes. B. Drug Identification and development [0104] The present invention provides a screening tool for the easy identification of interaction between a ligand and a target, opening up important perspectives m drug screening. 1. Measurement of the biological profile of compound libraries at nigh throughput [0105] High throughput screening of compound libraries is a major step the process of drug discovery. High throughput screening is often also required during the lead optimization phase as well as for drug development. Thus the object of the screening can either be to screen several thousands or even millions of compounds for their pharmacological activity on a target cr to test the toxic activity of a selected compound en a number of non-targets. Pharmaceutical, biotech or agro-cnemical companies have compiled collections of compounds from various sources m order to discover molecules that car be developed into potential drugs or agrochemicals . [0106] Botn pharmacological and toxic activities are the result of the interaction between compounds and biological components of the cellular machinery. Such interaction process is usually related to the binding of the compound to receptors, enzymes, DNA, structural proteins, membranes or any other biological molecule or structure. The intervention m the normal regulation cf cellular processes will induce one or more metabolic or structural modifications, which can be detected by measuring cellular enthalpy changes . [0107] Screening compound libraries ranging from 50000 to several millions of molecular entities requires a heavy investment m machinery, reagents and data analysis and usually ends up with the selection cf only a few molecules. [0108] Whereas coverage of the "chemical space" has been widely discussed and worked out m the design and selection of compound libraries, the "biological space" is rarely foreseen. Lipmsky (reference? ) has recently published the analysis of several hundreds of drugs and has come up with a list of five molecular parameters which may be required. Although this rule of five is now applied the selection and synthesis of compounds it does not guarantee the potential of such molecules to become drugs nor to exhibit biological activities. Indeed the compound selection according to these simple rules could even reduce the chances of discovery. [0109] It is an object of the present invention to detect the changes in cellular enthalpy (or of the surrounding environment) caused by the activity of the molecule on the target as a temperature change using micrccalorimetry. Moreover, the present invention makes it possible to perform such measurements in a high-throughput set-up. Additionally, by integrating pH metry in the device, the effect on the ionic balance can be recorded. In this way the "biological space" of any compound collection can be effectively assessed. [0110] Such method can refine any compound library. Screening only the biologically active molecules dramatically increase the probability to find pharmacologically active molecules. It will also reduce notably the amount of compounds to be screened. [0111] Different strategies for drug screening can be envisaged in the context of the present invention, depending on whether or not the target is well-defined. Preferably, a library of compounds is tested on the target . If the target is a known enzyme, this enzyme can be used either as the "sample" or the "stimulus" in the screening procedure. Alternatively, it can be envisaged that cell- cultures (unmodified or modified to express specific target molecules) can be used as sample to screen for a ligand which binds (and possibly also influence the activity of) the target. Possibly, the target is a protein of which the function is still unknown, and identification of a specific ligand can help assess the target function. [0112] Large compound collections can be made starting from natural small ligands . The molecule classes can comprise: amino acids (naturals and derivatives), carbohydrates (glucose, fructose, sucrose, etc.), lipids (fatty acids, Prostaglandins , leukotrienes , etc.) nucleotides (NTPs, NDPs , etc.), neurotransmitters (GABA, Serotonine, Dopamine, Glutamate, etc.), small peptides (NPY, vasopressin, etc.) , enzyme co-factors (e.g. NAD, FAD, NADPH, acetyl-CoA, etc.), and others. [0113] If the target is a known molecule it can be produced in a purified form and in bulk quantities. Screening plates are prepared by replication/dilution cf stock plates of the compound library and addition in duplicate in a microtiterpiate for screening. Control wells contain a known ligand cf the target. Upon addition of the target, heat change is monitored. An interaction between the target and a ligand will be detected by heat change; the corresponding ligand molecule is called a hit. [0114] Alternatively, the library of compounds can be tested on one or more cell types. Cultures of these cells are grown in an array of receiving zones of the device. The compounds of the potential drug library are applied as stimulus. In control receiving zones, a known stimulus can be added. When working with genetically modified cells as target samples, the control will be the non-modified cell- line. If a compound is active on the cell-line tested (binding to membrane, modifying membrane structure, entering the cell, disrupting cellular components, binding or interaction with intracellular molecules etc.) or in any way modifies the physiological state of the cell this will be measured as a temperature change in-or outside the cell . A library of known biologically active molecules (such as Tocris or RBI) can be used as a reference and to differentiate active molecules from the others. [0115] The corresponding molecule is selected as a hit and can easily be identified by its coordinates in the microtitre plate. Active molecules are labeled as positives in the related database; such hits can then be "picked" from the stock plates and re-distributec in new stock plates. This process can be done using automated equipment. The newly formated library can be called "enriched library" and commercialized or screened for more specific targets C. Identification cf Agrochemicals [0116] As mentioned above, the device cf the present invention can also be of interest m the screening of compounds m the identification of agrochemicals, particularly m the search for novel herbicides, pesticides and fungicides. Envisaged targets can be either plant, animal or fungal cells, respectively or can be specific target molecules which are known to be crucial for the normal function and development of these cells (such as DNA, RNA, enzymes involved m the synthesis of ammo acids, or proteins etc . ) . Libraries of compounds can be screened for their activity and the hits can be further developed into active compounds. Alternatively, the device of the invention can be used to screen libraries of structural variations of a Known compound that has been slightly modified m order to increase/decrease its specific or nonspecific (undesired) activity m the improvement of known chemicals. This can result m a significant reduction m time and effort needed compared to classical synthesis and testing approaches. P. Detection and Identification of bacteria [0117] Microbial contamination concerns are well documented and this problem is gaining momentum as more outbreaks caused by contaminated water or food are reported and greater populations are affected. The ma bacteria strains are E coll, Listeria, and Salmonella. Economic losses to the food production industry are significant as bacterial contamination shuts down processing and scours the reputation of businesses. To date, traditional culture plate methods have been used to monitor food production batches. Tnese methods are labour intensive and time consuming and, most importantly, require incubation times of 24-72 hours. The need for an easy, fast, accurate and sensitive bacterial detection method is immediate and crucial to maintaining public health. [0118] Moreover, the most sensitive assays to detect bacterial strains are based on ELISA which are time consuming and rather expensive. The API-ZYM™ system from biomerieux uses enzymatic profiling as a tool to not only detect bacteria but also provide detailed information about strains and/or species. The system is based on strain specific features concerning enzyme expression. Thus a combination of enzyme expression is designed to detect one bacteria specie. This test is usually performed on a barrette (see figure 7) where a combination of 19 enzymes allows rapid identification of bacteria. A similar system is used m Biolog MicroPlates™ . These are standard 96-well microplateε that are pre-loaded with metabolic tests for characterizing and/or identifying microbes. Each well of the microplate contains a different carbon source along with redox chemistry m a dry form. The chemistry is rehydrated by inoculation with a cell suspension, a procedure that takes less than 1 minute. Utilization of the carbon source m a well results a redox reaction, which forms a purple color or a turbidity change. [0119] Again, culture is required and sensitivity is an issue. [0120] Microcalorimetry appears then as the method of choice to quickly and accurately detect enzymatic activity from low amounts of bacteria. Ma erials: Test samples : [0121] Sterile cotton swabs are used to swipe the material to be screened (food, hands, surfaces, etc.), they are incubated in 15 ml tubes with 10 mi of incubation buffers; incubated under shaking for 15 minutes. The cotton swab is then removed and tubes are centrifuged at 20 000 rpm for 15 minutes to pellet all organisms. The pellet is resuspended in 100 μl of incubation buffer to prepare the test sample. Screening plate : The test plate is prepared as follows: [0122] A set of 19 specific enzyme substrates is diluted at a concentration of 5 μM and distributed in test wells of the microcalorimetry microplate; whereas reference wells contain an equivalent volume of incubation buffer. Procedure ; [0123] Microcalorimetry is monitored in real time in the test plate; 2 to 5 μl of the test sample is then added in all duplicate wells. The heat change generated from control wells is then registered. If any enzyme present in the test sample in a specific well reacts with a specific substrate; the change in heat will be detected within the microcalplate . Enzyme potency is function of the quantification of temperature change in time. Any bacterium that generates heat in a certain proportion of the control wells (to be determined in each case) is then considered a hit. The well co-ordinates will allow the identification of the corresponding enzyme Data processing: [0124] Data are processed m real time and stored m a database. The analysis is then carried to determine a specific enzyme combination that will allow, as a fingerprint, the fast identification of a pathogenic bacteria strain. [0125] Bacteria contamination and strain identification can thus be processed m less than 4 hours by enzymatic profiling, using microcalorimetry a microplate format. This process is likely to be automated using classical robotic equipment used m high throughput screening .

Claims: CLAIMS 1. An array device for monitoring the effect cf a physical cr chemical stimulus on multiple small samples, said array device comprising a supporting substrate at least two array elements that are separated from each other by a isolation zone, said array elements comprising : • A receiving zone arranged to provide a contact between said one of said samples and said physical or chemical stimulus, said receiving zone having a cross-section smaller than 10 mm, • A heat detection means arranged to perform a measurement of heat between said receiving zone and a reference, and said isolation zone being formed by at least part of said supporting substrate characterised in that said supporting substrate has sufficient strength to support said array device and said isolation zone is arranged to thermally isolate said array elements . 2. The array device of claim 1, wherein the reference is an external reference. 3. The array device of claim 1, wherein the reference is a neighbouring receiving zone. 4. The array device of claim 3, wherein said heat detection means is a differential heat detection means arranged to measure differences in heat between both receiving zones . 5. The array device of any of the claims 1 to 4, dimensioned as a standard 96, 384, 1536 or 6144-well microtiterplate . 6. The array of any of the claim 1 to 5, wherein said receiving zone comprises a resistor. 7. The array of any cf the claims 1 to 6, wherein said receiving zone comprise a bottom surface and sidewalls . 8. The array of claim 7, wherein said bottom surface comprises said supporting substrate. 9. The array of claim 7 or 8, wherein said bottom surface is perforated. 10. The array of any of the claims 1 to 9, wherein said isolation zone comprises a bottom surface, formed by said supportmg substrate, and an isolator comprised between the receiving zones . 11. The array of any of the claims 1 to 10, wherein said heat detection means is selected from the group consisting of thermopile, thermistor, diode, IR detection means, CCD camera. 12. The array of any of the claims 1 to 11, the receiving zone further comprising a detection means selected from the group consisting of a light addressable potentiometπc sensor, a pair of terdigitated electrodes, a field effect transistor, a diode, a reference electrode, and a working electrode. 13. The array of claim 1 wherein said receiving zone is chemically modified to physically contain said sample and said chemical or physical stimulus within said receiving zone. 14. The array of any of the claims 1 to 13 wherein the receiving zones have a cross-section of between 10 μm and 9 mm. 15. Calorimetric measuring method for measuring multiple samples, said method comprising the steps of : • Providing an array device such as m any of the claims 1 to 14, • Providing said samples said receiving zones, • Providing a chemical or physical stimulus to said samples and, • Measuring the heat released by said samples. 16. Use of the device of any of the claims 1 to 14 for enzyme discovery. 17. Use of the device of any of the claims 1 to 14 for drug identification. 18. Use of the device of any of the claims 1 to 14 for agrochemical identification.

Inventors: VERHAEGEN KATARINA, VERHAEGEN, KATARINA
Applicants: IMEC INTER UNI MICRO ELECTR, VERHAEGEN KATARINA, INTERUNIVERSITAIR MICRO-ELEKTRONICA CENTRUM, VERHAEGEN, KATARINA
Application year: 2001
Main classification: C12M1
IPC codes: C12M1, C12N15, G01K7, G01N25, G01N33, G01N37