OXAZOLIDINONE PHOTOAFFINITY PROBES

Abstract: Disclosed are novel compounds that are useful and effective as photoaffinity probes and methods of using oxazolidinone photoaffinity probes.

Description: OXAZOLIDINONE PHOTOAFFINITY PROBES FIELD OF THE INVENTION The present invention is directed, in part, to novel photolabile oxazolidinones and preparation thereof. The novel compounds can be used as photoaffinity probes within sensitive bacteria including both gram-positive and gram-negative bacteria and mammalian cells. BACKGROUND OF THE INVENTION A number of antibiotic compounds have been developed and shown to be effective in inhibiting translation. The antibiotics neomycin, thiostrepton, and hygromycin appear to inhibit translocation and occupation of the A site within a ribosome but have no effect on formation of the fMet-tRNA f Met /ribosome translation complex nor on the peptide-bond synthesis which occurs in the absence of elongation factor P. Streptomycin, which causes misreading and also inhibits A-site binding, interacts with two sites on the 16S rRNA of the 30S subunit. Lincomycin inhibits peptidyltransf erase and occupation of the A-site. Erythromycin inhibits peptidyltransf erase and destabilizes the peptidyl-tRNA/ribosome/mRNA complex. A number of additional compounds have been recently developed and have been shown to act as antimicrobial or antibacterial agents. International Publication WO 99/41244 discloses substituted aminophenyl isoxazoline compounds useful as antimicrobial agents. U.S. Patent No. 5,910,504 describes hetero-aromatic ring substituted phenyloxazolidinone antimicrobial agents. In addition, International Publication WO 00/10566 discloses isoxazolinone antibacterial agents. An important step in the development of new antimicrobial or antibacterial agents, such as those disclosed above, is the elucidation of a mechanism of action. The specific site of interaction of non selective antibiotics/antitumor agents, such as sparsomycin, that inhibit protein translation by a different, less useful, and direct mechanism, has been described. Porse et al. , Proc. Natl. Acad. Sci. USA, 1999, 96, 9003-9008. Previous studies with chemical probes using isolated, cell-free systems have failed to define the relative sites of interaction of these types of antibiotic compounds of the oxazolidinone class. Matassova, et al, RNA, 1999, 5, 939-946. This is, in part, because the previous methods were incapable of defining the sites of the particular and specific mechanism of action of this important class of antibiotics. Probes that help to elucidate the mechanism of action of antimicrobial and/or antibacterial agents and methods of using the same are highly desired. The present invention is directed, inter alia, to novel oxazolidinone compounds and use of oxazolidinone compounds as photoaffinity probes. Compounds suspected of having antimicrobial and/or antibacterial activity can be used in intact cells and can be evaluated for a mechanism of action by using active and inactive enantiomers of oxazolidinone compounds as competitors for crosslinking to components within the cell. The compounds and methods of the present invention allow one skilled in the art to elucidate the mechanism of action of antibacterial and/or antimicrobial agents that are suspected of inhibiting protein translation. These and other aspects of the invention are described below. SUMMARY OF THE INVENTION The present invention is directed to novel compounds that are useful and effective as photoaffinity probes useful for, inter alia, identification of the oxazolidinone binding site within gram-positive and gram-negative bacteria. These compounds can also be used for aiding in the determination of oxazolidinone binding sites within mammalian cells. The compounds of the present invention comprise Formula I shown below. Formula I wherein X and Y are, independently, F, H or CH 3 ; R 1 is H, F or I; R 2 is H, F or OH; R 16 is H or F; R 17 is H or F; R 3 is H or Cι-C 8 alkyl; L is a bond or -OCH 2 C(=O); and Q is wherein R is H, CH 3 , CH 2 CH 3 or cyclopropyl; and Z is O or S; or a pharmaceutically acceptable salt thereof. Other compounds of the present invention comprise Formula II shown below. Formula II wherein X and Y are, independently, F, H or CH 3 ; R 1 is H, I or F; R 2 is H, OH or F; R 16 is H or F; R 17 is H or F; and Q is wherein R is H, CH 3 , CH 2 CH 3 or cyclopropyl; and Z is O or S; or a pharmaceutically acceptable salt thereof. Other compounds of the present invention comprise Formula TΩ. shown below. Y wherein X and Y are, independently, F, H or CH 3 ; R is wherein R 6 is H, N 3 , halogen, NH 2 , OH, SH, -C 4 alkylamino, C 1 -C 4 dialkylamino, Cι-C alkyl, nitrile, carboxamide, C1-C 4 alkoxy, C 1 -C 4 alkylthio, or C 1 -C 4 alkoxycarbonyl; and P is wherein Z is O or S; and R 7 is wherein R 1 is H, F or I; and R 2 is H, F or OH; R 16 is H or F; R 17 is H or F; or a pharmaceutically acceptable salt thereof. In other embodiments of the invention, methods of using a compound comprising Formula IN as a photoaffinity probe are provided, wherein Formula IV is Formula IN wherein X and Y are, independently, F, H or CH 3 ; R 8 is H, F or I; R 9 is H, F or OH; R 18 is H or F; R , 19 is H or F; R , 1 1 0 U i: s H or Cι-C 8 alkyl; L is a bond or -OCH 2 C(=O); and Q is wherein R 11 is H, CH 3 , CH 2 CH 3 or cyclopropyl; and Z is O or S; or a pharmaceutically acceptable salt thereof. In other embodiments of the invention, methods of using a compound comprising Formula N as a photoaffinity probe are provided, wherein Formula N is Formula N wherein X and Y are, independently, F, H or CH 3 ; R 12 is Ν 3 or wherein R 8 is H, I or F; R 9 is H, OH or F; R 18 is H or F; R 19 is H or F; and Q is wherein R 11 is H, CH 3 , CH CH 3 or cyclopropyl; and Z is O or S; or a pharmaceutically acceptable salt thereof. In other embodiments of the invention, methods of using a compound comprising Formula VI as a photoaffinity probe are provided, wherein Formula VI is Y Formula VI wherein X and Y are, independently, F, H or CH 3 ; R 13 i •s wherein R , 1 ; is H, N 3 , halogen, NH 2 , OH, SH, Cι-C alkylamino, Cι-C 4 dialkylamino, Cι-C 4 alkyl, nitrile, carboxamide, Cι-C 4 alkoxy, Cι-C 4 alkylthio, or Cι-C 4 alkoxycarbonyl; and P is wherein: Z is O or S; and R 15 is wherein R 8 is H, F or I; and R 9 is H, F or OH; R 18 is H or F; R , 1 ι 9 y is H or F; or a pharmaceutically acceptable salt thereof. DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS Various definitions are made throughout this document. Most words have the meaning that would be attributed to those words by one skilled in the art. Words specifically defined either below or elsewhere in this document have the meaning provided in the context of invention as a whole and as are typically understood by those skilled in the art. As used herein, the term "cross-linking" or "binding" means the physical interaction between the photoaffinity probe and at least one component within a cell or from a cell or combinations thereof. Binding includes ionic, non-ionic, Hydrogen bonds, Van der Waals, hydrophobic interactions, etc. The physical interaction, the binding, can be either direct or indirect, indirect being through or because of another protein or compound. Direct binding refers to interactions that do not take place through or because of another protein or compound but instead are without other substantial chemical intermediates. As used herein, the term "component" within a cell means any protein, nucleic acid, lipid, etc. within a cell. Components include, but are not limited to, the contents of the cytoplasm, nucleus, cell membrane, cell wall, and the like. As used herein, the term "competitor compound" means any identifiable chemical or molecule, small molecule, peptide, protein, sugar, natural or synthetic, that is suspected (such as a test compound) to potentially interact with or compete with the photoaffinity probe for cross- linking to a component within a cell or from a cell. As used herein, the term "contacting" means either direct or indirect, application of a photoaffinity compound or competitor compound within a cell, on or to a cell, or to components from a cell. The competitor compound or photoaffinity compound can be present within a buffer, salt, solution, etc. As used herein, the term "oxazolidinone" means a compound of the class known as oxazolidinones, including the compounds described in U.S. Serial Numbers 07/438J59, 07/553,795, 07/786,107, 07/831,213, 08/329,717, 07/909,387, 60/015,499, 09/138,209, 60/008,554, 60/064,738, 60/065,376, 60/067,830, 60/089,498, 60/100,185, 60/088,283, 60/092,765, 07/244,988, 07/253,850; European Patents EP 0500686, EP 0610265, EP 0673370; PCT Application Numbers PCT/US90/06220, PCT/US94/08904, PCT/US94/10582, PCT/US95/02972, PCT/US95/10992, PCT/US93/04850, PCT/US95/12751, PCT/US96/00718, PCT/US93/03570, PCT/US93/09589, PCT/US96/05202, PCT/US97/03458, PCT/US96/12766, PCT/US97/01970, PCT/US96/14135, PCT/US96/19149, PCT/US96/17120, PCT/US98/09889, PCT/US98/13437;and U.S. Patent Numbers 5,700,799, 5,719,154, 5,547,950, 5,523,403, 5,668,286, 5,652,238, 5,688,792, 5,247,090, 5,231,188, 5,654,428, 5,654,435, 5,756,732, 5,164,510, 5,182,403, 5,225,565, 5,618,949, 5,627,197, 5,534,636, 5,532,261, 5,776,937, 5,529,998, 5,684,023, 5,627,181, 5,698,574, 5,220,011, 5,208,329, 5,036,092, 4,965,268, 4,921,869, 4,948,801, 5,043,443, 5,130,316, 5,254,577, 4,877,892, 4,791,207, 4,642,351, 4,665,171, 4,734,495, 4,775,752, 4,870,169, 4,668,517, 4,340,606, 4,362,866, 4,193,918, 4,000,293, 3,947,465, 4,007,168, 3,674,780, 3,686,170, 3,906,101, 3,678,040, 3,177,114, 3,141,889, 3,149,119, 3,117,122, 5,719,154, 5,254,577, 4,801,600, 4,705,799, 4,461,773, 4,243,801, 3,794,665, 3,632,577, 3,598,830, 3,513,238, 3,598,812, 3,546,241, 3,318,878, 3,322,712, 5,565,571, 5,880,118, 5,952,324, 5,910,504, 6,166,056, 5,968,962, 6,090,820, 5,736,545, 6,277,985, 5,955,460, 5,922,7076,255,304, 6,218,413, 5,977,373, 6,251,869, 5,929,248, and 5,801,246; the disclosures of each of which are incorporated herein by reference in their entirety. Preferred oxazolidinones include linezolid and eperezolid. The present invention is directed to novel photoaffinity probes comprising Formula I, Formula II, or Formula HI. The preferred configuration at C-5 is (S). It will be appreciated by those skilled in the art that compounds of the present can have additional chiral centers and be isolated in optically active or racemic form. The present invention encompasses any racemic, optically-active (such as enantiomers, diastereomers), tautomeric, or stereoisomeric form, or mixture thereof, of a compound of the invention. The present invention is also directed to compositions comprising photoaffinity probes which comprise Formula I, Formula π, or Formula HI, or a mixture thereof. Preferred compounds of this invention have one radioactive element which is either 3 H (T 3 ), S, or I. It is understood, however, that the Formulas include all isotopic forms of the compounds depicted. In some embodiments of the invention, compounds comprise Formula I, shown below. Formula I wherein X and Y are, independently, F, H or CH 3 in a variety of substitution patterns. Preferred compounds have one fluorine and one H. R 1 is H, F or I. R 2 is H, F or OH. R 16 is H or F. R 17 is H or F. R 3 is H or C x -C 8 alkyl. L is a bond or -OCH 2 C(=O). Q is wherein R 4 is H, CH 3 , CH 2 CH 3 or cyclopropyl. Z is O or S. Compounds comprising Formula I also include pharmaceutically acceptable salts thereof. Preferred compounds comprising Formula I have the following substituents: X is F, Y is H, R 3 is H, and R 4 is CH 3 . More preferably, compounds of Formula I include, but are not limited to , 2- [4- [4- [(5S)-5- [( Acetylamino)methyl] -2-oxo-3 -oxazolidinyl] -2-fluorophenyl] - 1 -piperazinyl] - 2-oxoethyl-4-azido-2-hydroxy-5-iodo- 125 I-benzoate, N-[[(5S)-3-[4-[4-(4-Azido-2-hydroxy-5- iodo- 125 I-benzoyl)-l-piperazinyl]-3-fluorophenyl]-2-oxo-5-oxazolidinyl]methyl] acetamide, 2-[4-[4-[(5S)-5- [( Acetylamino)methyl] -2-oxo-3 -oxazolidinyl] -2-fluorophenyl] - 1 - piperazinyl]-2-oxoethyl 4-azido-3-iodo- 125 I-benzoate, and N-[[(5S)-3-[4-[4-(4-Azido-3-iodo- 125 I- benzoyl)- 1 -piperazinyl] -3 -fluorophenyl] -2-oxo-5-oxazolidinyl]methyl] acetamide. In other embodiments of the invention, compounds comprise Formula π, shown below. Formula π wherein X and Y are, independently, F, H or CH 3 in a variety of substitution patterns. Preferred compounds have one fluorine and one H. R 1 is H, I or F. R 2 is H, OH or F. R 16 is H or F. R 17 is H or F. Q is wherein R is H, CH , CH 2 CH 3 or cyclopropyl. Z is O or S. Compounds comprising Formula II also include pharmaceutically acceptable salts thereof. Preferred compounds comprising Formula II have the following substituents: X is F, Y is H, and R 4 is CH 3 . More preferably, compounds of Formula II include, but are not limited to, N-[[(5S)-3-(4'-Azido-2-fluoro[l,r-biphenyl]-4-yl)-2-oxo-5-oxazolidinyl]methyl]-T 3 -acetamide, N-[[(5S)-3-(4'-Azido-2-fluoro-3'-iodo[l,l'-biphenyl]-4-yl)-2-oxo-5-oxazolidinyl] methyl]-T 3 -acetamide, N-[[(5S)-3-(4'-Azido-2-fluoro-3'-iodo[l,l'-biphenyl]-4-yl)-2-oxo-5- oxazolidinyl]methyl]ethane- 35 S-thioamide, and N-[[(5S)-3-(4' -Azido-2-fluoro-3 ' -iodo- 125 I-[l , 1 ' - biphenyl]-4-yl)-2-oxo-5-oxazolidinyl]methyl]acetamide. In other embodiments of the invention, compounds comprise Formula HI, shown below. Y Formula HI wherein X and Y are, independently, F, H or CH 3 in a variety of substitution patterns. Preferred compounds have one fluorine and one H. R is wherein R 6 is H, N 3 , halogen, NH 2 , OH, SH, C C 4 alkylamino, -C 4 dialkylamino, C C 4 alkyl, nitrile, carboxamide, Cι-C alkoxy, Cι-C 4 alkylthio, or C 1 -C 4 alkoxycarbonyl. P is wherein Z is O or S. R 7 is wherein R 1 is H, F or I. R 2 is H, F or OH. R 16 is H or F. R 17 is H or F. Compounds comprising Formula HI also include pharmaceutically acceptable salts thereof. Preferred compounds comprising Formula HI have the following substituents: X is F, Y is H, and R 6 is H. More preferably, compounds of Formula HI include, but are not limited to, (2E)-3-(4-azido-3-iodo- 125 I-phenyl)-N-[[(5S)-3-[3-fluoro-4-(4-pyridinyl)phenyl]-2-oxo-5- oxazolidinyl]methyl]-2-propenamide, 4-azido-N-[[(5S)-3-[3-fluoro-4-(4-pyridinyl)phenyl]-2- 19*. oxo-5-oxazolidinyl]methyl]-2-hydroxy-5-iodo- I-benzamide, and N-(4-azidophenyl)-N'-[[(5S)- 3- [3 -fluoro-4-(4-pyridinyl)phenyl] -2-oxo-5 -oxazolidinyl]methyl] - S -thiourea. The present invention is also directed to methods of using a compound having Formula I, H, HI, IV, V, or VI (shown above) as a photoaffinity probe. Briefly, a cell, or component(s) thereof, is contacted with a photoaffinity probe comprising Formula I, H, HI, IV, V, or NL Preferably, the photoaffinity probe is radiolabled. The photoaffinity probe is then exposed to light, preferably ultraviolet, in order to activate the photoaffinity probe. Cross-linking of the photoaffinity probe is determined by methods well known to those skilled in the art such as, for example, by detecting the radiolabel. Radiolabel, such as H, I, or S, for example, can be detected by a variety of autoradiography techniques well known to the skilled artisan. In other embodiments of the invention, the method further comprises contacting the cell or component(s) thereof with a competitor compound. The ability of the competitor compound to interfere with cross-linking of the photoaffinity probe indicates the bioactivity of the competitor compound. Cells of the present invention include, but are not limited to, gram positive bacterial pathogens, including, for example, Staphylococcus aureus; Staphylococcus epidermidis (A, B, C biotypes); Staphylococcus caseolyticus; Staphylococcus gallinarum; Staphylococcus haemolyticus; Staphylococcus hominis; Staphylococcus saprophyticus; Streptococcus agalactiae (group B); Streptococcus mutans/rattus; Streptococcus pneumoniae; Streptococcus pyogenes (group A); Streptococcus salivarius; Streptococcus sanguis; Streptococcus sobrinus; Actinomyces spps.; Arthrobacter histidinolovorans; Corynebacterium diptheriae; Clostridium difficle; Clostridium spps.; Enterococcus casseliflavus; Enterococcus durans; Enterococcus faecalis; Enterococcus faecium; Enterococcus gallinarum; Erysipelothrix rhusiopathiae; Fusobacterium spps.; Listeria monocytogenes; Prevotella spps.; Propionibacteήum acnes; and Porphyromonas gingivalis. Cells also include, but are not limited to, gram negative bacterial pathogens, including, for example, Acinetobacter calcoaceticus; Acinetobacter haemolyticus; Aeromonas hydrophila; Bordetella pertussis; Bordetella parapertussis; Bordetella bronchiseptica; Bacteroides fragilis; Bartonella bacilliformis; Brucella abortus; Brucella melitensis; Campylobacter fetus; Campylobacter jejuni; Chlamydia pneumoniae; Chlamydia psittaci; Chlamydia trachomatis; Citrobacter freundii; Coxiella burnetti; Edwardsiella tarda; Edwardsiella hoshinae; Enterobacter aerogenes, Enterobacter cloacae (groups A and B); Escherichia coli (to include all pathogenic subtypes) Ehrlicia spps. ; Francisella tularensis; Haemophilus actinomycetemcomitans; Haemophilus ducreyi; Haemophilus haemolyticus; Haemophilus influenzae; Haemophilus parahaemolyticus; Haemophilus parainfluenzae; Hafnia alvei; Helicobacter pylori; Kingella kingae; Klebsiella oxytoca; Klebsiella pneumoniae; Legionella pneumophila; Legionella spps.; Morganella spps.; Moraxella cattarhalis; Neisseria gonorrhoeae; Neisseria meningitidis; Plesiomonas shigelloides; Proteus mirabilis; Proteus penneri; Providencia spps.; Pseudomonas aeruginosa; Pseudomonas species; Rickettsia prowazekii; Rickettsia rickettsii; Rickettsia tsutsugamushi; Rochalimaea spps. ; Salmonella subgroup 1 serotypes (to include S. paratyphi and S. typhi); Salmonella subgroups 2, 3a, 3b, 4, and 5; Serratia marcesans; Serratia spps.; Shigella boydii; Shigella flexneri; Shigella dysenteriae; Shigella sonnei; Yersinia enterocolitica; Yersinia pestis; Yersinia pseudotuberculosis; Vibrio cholerae; Vibrio vulnificus; and Vibrio parahaemolyticus. Cells also include, but are not limited to, Mycobacterial species, including, for example, Mycobacterium tuberculosis; Mycobacterium avium; and other Mycobacterium spps. Cells also include, but are not limited to, Mycoplasmas (or pleuropneumonia-like organisms), including, for example, Mycoplasma genitalium; Mycoplasma pneumoniae; and other Mycoplasma spps. Cells also include, but are not limited to, Treponemataceae (spiral organisms) including, for example, Borrelia burgdorferi; other Borrelia species; Leptospira spps. ; Treponema pallidum. Methods for preparing the photoaffinity probes described in Formulas I, H, HI, IN, N, and NI are depicted in the following synthesis schemes. It will be apparent to those skilled in the art that the described synthetic procedures are merely representative in nature and that alternative procedures are feasible and may be preferred in some cases. Νon-radioactive compounds of Formulas I and IN are prepared by the methods described in Schemes A and B. As shown in Scheme A, coupling of a benzoic acid moiety (Ai) with an appropriate hydroxyacetyl piperazine fragment (A 2 ) leads to compounds A 3 of Formula I where L is -OCH 2 C(=O). Coupling can be accomplished with l-[3-(dimethylamino)propyl]-3- ethylcarbodiimide hydrochloride or any other reagents familiar to ones skilled in the art. Appropriate benzoic acid fragments can be made by procedures known in the literature. (Dupuis, Can. J. Chem., 1987, 65, 2450-2453; Shu, /. Labelled Compounds and Radiopharmaceuticals, 1996, 38, 227-237, each of which is incorporated herein by reference in its entirety). Appropriate hydroxyacetyl piperazine fragments can also be made by methods known in the literature (Barbachyn, U.S. Patent No. 5,547,950; Barbachyn, U.S. Patent No. 5,990,136; and Synder, Int'l Publication WO 00/10566-A1, each of which is incorporated herein by reference in its entirety). Methods for incorporation of 125 I into compounds A 3 are shown in Schemes C and D. Scheme A can also be used where the compounds of Ai and A 3 have R and R substituents ortho to the acid substituent (as in Formulas I and IN). Scheme A: Non-radioactive compounds of Formulas I and IN where L is a bond are prepared by the synthetic sequence shown in Scheme B. An appropriate benzoic acid fragment (Ai of Scheme A) is coupled with an appropriate piperazine (B 2 ) using 1,1-carbonyldiimidazole in tetrahydrofuran to give the desired compound (B 3 ). Other coupling methods known to those skilled in the art are also possible. The piperazine fragment is made by methods known in the literature (Hutchinson, U.S. Patent No. 5,700,799, which is incorporated herein by reference in its entirety; Barbachyn, U.S. Patent No. 5,990,136; and Snyder, International Publication WO 00/10566-A1). Methods for incorporation of 125 I into compounds B 3 are shown in Schemes C and D. Scheme B can also be used where the compound of B 3 has R 16 and R 17 substituents ortho to the amide substituent (as in Formulas I and IN). Scheme B: Bo B 3 Radioactive iodine is introduced into the compounds of Formulas I and IV by the methods shown in Schemes C and D. Compounds C 2 of Formula I (where R 1 is OH and R 2 is 19 I) are prepared by reaction of compounds Ci (prepared according to the methods of Schemes A and B) with Νa 125 I and chloramine-T. Scheme C: c, c 2 1 9 1 *. Alternatively, compounds D 3 of Formulas I and IV (where R is H and R is I) are prepared as shown in Scheme D. Reaction of compounds Di (prepared by the methods shown in 1 < Schemes A and B) with hexamethylditin affords the stannanes D 2 . Reaction of D 2 with Na I and chloramine-T leads to D 3 . Scheme D: Non-radioactive compounds of Formulas H and V are prepared by the method shown in Scheme E. The appropriate biphenyl nitro fragment (Ei) is reduced in the presence of hydrogen gas and a palladium catalyst to give the appropriate biphenyl aniline fragment (E 2 ). Other reduction methods familiar to those skilled in the art may also be used. Conversion to the azido moiety (E 3 ) can be accomplished via displacement of the appropriate diazonium salt with sodium azide using conditions familiar to those skilled in the art. The appropriate nitro fragments (E_) can be prepared by methods known in the literature (Barbachyn, U.S. Patent No. 5,654,435, which is incorporated herein by reference in its entirety; Barbachyn U.S. Patent No. 5,990,136; and Synder, International Publication WO 00/10566-A1) or by other methods familiar to those skilled in the art. Introduction of radioactive elements into compounds of Formulas H and V are depicted in Schemes F, G, and H. Scheme E can also be used where the compounds of E ls E 2 , and E 3 have R 16 and R 17 or R 18 and R 19 substituents in the ortho position (as in Formulas H and V). Scheme E: Scheme F shows the procedure for incorporation of tritium into compounds of Formulas H and V where Q is oxazolidinone, Z is O, and R 4 is CH 3 . Reaction of Fi (prepared according to Scheme E) with 6N HC1 and methanol affords the free amine F 2 . Reaction of F with tritiated sodium acetate and a coupling reagent affords the tritiated acetamide F 3 . Suitable coupling reagents include O-benzotriazol-l-yl-N,N,N',N',tetramethyluronium hexafluorophosphate and O-(7-azabenzotriazol- 1 -yl)-N,N,N' ,N' -tetramethyluronium hexafluorophosphate. Other acceptable coupling reagents are known by those skilled in the art. Alternatively, tritiated acetic anhydride and a suitable base can be used in place of tritiated sodium acetate and a coupling reagent. Incorporation of tritium into compounds of Formulas H and V where Q is isoxazoline is carried out in similar fashion. Scheme F can also be used where the compounds of F ls F 2 , and F 3 have R and R or R and R substituents in the ortho position (as in Formulas H and V). Scheme F: Scheme G shows the method for incorporation of S into compounds of Formulas H here Q is oxazolidinone, Z is S, and R 4 is CH 3 . Reaction of F (from Scheme F) with ethyl 35 Sc-d.i+th;ioa. ce <t-a„*te a εftfo~r.dA„s * the„ 35 Sn- + thioace +ta„»m--id Ae~, n G 2 , τ Inco „rp-o„r.-a„<t..ion o εf 35 S c into compounds of Formulas H and V where Q is isoxazoline is carried out in similar fashion. Scheme G can also be used where the compound of G 2 has R 16 and R 17 or R 18 and R 19 substituents in the ortho position (as in Formulas H and V). Radioactive iodine can be introduced into compounds of Formulas H and V by the method shown in Scheme H. Reaction of Hi (prepared according to the route shown in Scheme 19^ E) with hexamethylditin affords the organostannane H 2 . Reaction of H with Na I and chloramine-T affords the radioiodinated compound H 3 . Scheme H: Compounds of Formula V where R 12 is N 3 are made according to the procedures described in U.S. Patent No. 5,910,504, Example 17, which is incorporated herein by reference in its entirety. Scheme I illustrates a synthetic method for the preparation of non-radioactive compounds of Formulas HI and VI where P is oxazolidinone, Z is O, and R 7 is optionally substituted azidophenyl or azidocinnamoyl. Refluxing an appropriate acetamide fragment (10 in methanolic hydrochloric acid affords the free amine I . The acetamide fragments (Ij) are prepared by methods known in the literature (Barbachyn, U.S. Patent No. 5,565,571, which is incorporated herein by reference in its entirety; Barbachyn, U.S. Patent No. 5,990,136; and Synder, International Publication WO 00/10566-A1). Coupling of I 2 with an appropriate benzoic acid fragment (I 3 , n = 0) or cinnamic acid fragment (I 3 , n = 1) leads to the amide L>. Coupling can be accomplished with EDC or other reagents familiar to ones skilled in the art. Appropriate benzoic acid fragments (I 3 , n = 0) are prepared by the same method used to prepare Ai of Scheme A. Appropriate cinnamic acid fragments (I 3 , n = 1) can be prepared by coupling of an appropriate benzaldehyde with Wittig-Horner reagents. Benzaldehyde fragments can be prepared by procedures known in the literature (Shu, /. Labelled Compounds and Radiopharmaceuticals, 1996, 38, 227-237) or by other methods familiar to those skilled in the art. Compounds of Formulas HI and VI where P is isoxazoline or isoxazolinone are made by similar methods. Scheme I can also be used where the compounds of I 3 and I 4 have R 16 and R 17 or R 18 and R 19 substituents in the ortho position (as in Formulas HI and VI). Scheme I: Introduction of 125 I into compounds of Formulas HI and VI where P is oxazolidinone, Z is O, and R 7 is optionally substituted azidophenyl or azidocinnamoyl is accomplished by the method shown in Scheme J. Reaction of I 4 (from Scheme I, where R 1 is H and R 2 is OH) with Na 125 I and chloramine-T affords the radioiodinated compound J 2 . Introduction of 125 Iinto compounds of Formulas HI and VI where P is isoxazoline or isoxazolinone is carried out by similar methods. Scheme J: Alternatively, introduction of 1 l 2 Δ 5τl into compounds of Formulas HI and VI where P is oxazolidinone, Z is O, and R 7 is optionally substituted azidophenyl or azidocinnamoyl is carried out by the method shown in Scheme K. Reaction of I 4 (from Scheme I, where R 1 is I and R 2 is H) with hexamethylditin affords the organostannane K 2 . Reaction of K with Na 125 I and chloramine-T affords the radioiodinated compound K 3 . Radioiodination of compounds of Formulas HI and VI where P is isoxazoline or isoxazolinone is carried out in similar fashion. Scheme K: Scheme L illustrates a synthetic method for the preparation of radioactive compounds of Formulas HI and VI where P is oxazolidinone, Z is S, and R is optionally substituted azidoaniline. An appropriate S-isothiocyanate is reacted with the appropriate aminomethyl fragment I (Scheme I) in refluxing THF to give the desired 35 S-thiourea, (L ). The required 35 S- isothiocyanate L 2 is prepared by reaction of an appropriate aniline with 35 S-thiophosgene. Introduction of S into compounds of Formulas HI and VI where P is isoxazoline or isoxazolinone is canied out in similar fashion. Scheme L can also be used where the compounds of L2. and L 3 have R 16 and R 17 or R 18 and R 19 substituents in the ortho position (as in Formulas HI and VI). Scheme L: The invention is further illustrated by way of the following examples which are intended to elucidate the invention. These examples are not intended, nor are they to be construed, as limiting the scope of the disclosure. EXAMPLES Example 1: Synthesis 2- [4- [4- [(5S )-5- [(Acetylamino)methyl]-2-oxo-3 -oxazolidinyl] -2-fluorophenyl] - 1 - piperazinyl] -2-oxoethyl 4-azido-2-hydroxy-5-iodo- 125 I-benzoate (Compound C 2 of Scheme C where L is -CH C(=O), X is F, Y is H, Q is oxazolidinone, Z is O and R 4 is CH ) is prepared as follows. Step 1. To a stirred solution of (S)-N-[[3-[3-fluoro-4[4-(hydroxyacetyl)-l-piperazinyl] phenyl]-2-oxo-5-oxazolidinyl]methyl]acetamide (515.8 mg, 1.31 mmol) in dimethylformamide (10 ml) and pyridine (1 ml) is added l-[3-(dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride (509.9 mg, 2.66 mmol) followed by 4-azidosalicylic acid (Dupuis, Can. J. Chem., 1987, 65, 2450-2453) and a catalytic amount of 4-dimethylaminopyridine. The reaction mixture is stined at room temperature for 72 hours then concentrated. The residue is diluted with CH 2 CI 2 (100 ml) and is washed successively with H 2 O (2 x 30 ml), 1 N HC1 (2 x 30 ml), saturated NaHCO 3 (1 x 30 ml), dried (MgSO 4 ), filtered and concentrated. The residue is dissolved in CH 3 OH/CH 2 Cl 2 , absorbed onto silica gel and is purified on a Biotage 40S column with a SIM using 2.5 % CH 3 OH in CH 2 C1 2 as the eluent to give 186.5 mg (0.33 mmol, 25 %) of the benzoate ester, mp 177-178 °C (dec). 1H-NMR (DMSO) δ: 10.4, 8.24, 7.87, 7.53, 7.17, 7.09, 6.76, 6.70, 5.17, 4.71, 4.09, 3.71, 3.60, 3.40, 3.02, 2.96, 1.83. Step 2. All reagents are prepared in 0.1 N NaPO 4 buffer, pH 7.4 unless otherwise specified. Buffer (70 μl), chloramine-T (70 μl of a 1 mM stock solution), and the azido phenol of Step 1 (10 μl of a 50 μM stock solution in DMSO) are added to a 1.5 ml glass reaction vial. A rubber septum cap is crimped onto the reaction vial and a solution of I 2 in sodium hydroxide (10 μl containing 1 mCi (Amersham #IMS 30) is added. The reaction is gently vortexed in the dark for 2 hours at room temperature then quenched with 10% solution of sodium bisulfite (100 μl). The quenched reaction is diluted with buffer (800 μl) and transferred from the reaction vial with a 1 ml tuberculin syringe fitted with an 18 gauge needle. The reaction volume (1 ml) is loaded onto a preconditioned C18 sep-pak cartridge (Millipore Corporation) and the 19 s . unincorporated I 2 is washed from the C18 resin with HPLC grade water containing 0.1% trifluoroacetic acid (20 ml). Product is eluted using of 80% CH 3 CN/0.1 TFA (3 ml). The typical yield of iodinated product is approximately 30% of the total 19 I 2 added to the reaction. Example 2: Synthesis N-[[(5S)-3-[4-[4-(4-Azido-2-hydι-oxy-5-iodo- 125 I-benzoyl)-l-piperazinyl]-3- fluorophenyl]-2-oxo-5-oxazolidinyl]methyl]acetamide (Compound C 2 of Scheme C where L is a bond, X is F, Y is H, Q is oxazolidinone, Z is O and R 4 is CH 3 ) is prepared as follows. Step 1. To a stined suspension of (S)-N-[[3-[4-[3-fluoro-4-(l-piperazinyl)]phenyl]-2- oxo-5-oxazolidinyl]methyl]-acetamide (498.0 mg, 1.3 mmol) in CH 2 θ 2 (10 ml) is added diisopropylethylamine (0.70 ml, 4.0 mmol) followed by 4-azidosalicoyl chloride (342.6 mg, 1.7 mmol) in CH 2 CI 2 (7 ml). The reaction mixture is stirred at room temperature for 18 hours and then is partitioned between CH 2 C1 2 (50 ml) and H 2 O (10 ml). The phases are separated. The organic layer is washed with H 2 O (10 ml), dried (MgSO 4 ), filtered and concentrated. The residue is dissolved in CH 3 OH/CH2θ 2 , absorbed onto silica gel and is purified on a Biotage 40S column with a SIM using 3% CH 3 OH in CH 2 C1 2 as the eluent to afford 412.3 mg (0.83 mmol, 62%) of the desired benzamide as a tan solid, mp 188-189 °C (dec). 1H-NMR (DMSO) δ: 10.2, 8.24, 7.51, 7.22, 7.16, 7.07, 6.64, 6.58, 4.70, 4.07, 3.70, 3.36, 2.96, 1.83. Step 2. Starting with the phenol prepared in Step 1, 125 I is introduced according to the procedure described in Step 2 of example 1. Example 3: Synthesis 2-[4-[4-[(5S)-5-[(Acetylamino)methyl]-2-oxo-3-oxazolidinyl]-2-fluorophenyl]-l- piperazinyl]-2-oxoethyl 4-azido-3-iodo- 125 I-benzoate (Compound D 3 of Scheme D where L is - CH 2 C(=O), X is F, Y is H, Q is oxazolidinone, Z is O and R 4 is CH 3 ) Step 1. To a stined solution of 4-azido-3-iodobenzoic acid (103.8 mg, 0.36 mmol, (Shu, /. of Labelled Compounds and Radiopharmaceuticals, 1996, 38, 227-237)) in dry THF (2.0 ml) is added 1,1-carbonyldiimidazole (58.2 mg, 0.36 mmol). The reaction mixture is stined at room temperature for 1 hour, then (S)-N-[[3-[3-fluoro-4[4-(hydroxyacetyl)-l-piperazinyl]phenyl]-2- oxo-5-oxazolidinyl]methyl]acetamide (141.9 mg, 0.36 mmol) is added followed by a catalytic amount of DMAP. The reaction mixture is heated at reflux for 72 hours. The reaction mixture was cooled to room temperature and poured into CH 2 CI 2 (30 ml) and washed successively with H 2 O (15ml), 1 N HC1 (15 ml), saturated aqueous NaHCO 3 (15 ml), brine (15 ml), dried (MgSO 4 ), filtered and concentrated. The residue is purified on a Biotage 12M column using 2 % CH 3 OH in CH 2 CI 2 as the eluent to afford 119.6 mg (0.18 mmol, 50%) of the benzoate ester, mp 137-139 °C. 1H-NMR (CDC1 3 ) δ: 8.52, 8.14, 7.49, 7.19, 7.07, 6.95, 6.01, 5.00, 4.72, 4.02, 3.81, 3.75, 3.62, 3.05, 1.58. Step 2. To a stirred solution of the iodobenzoate prepared in Step 1 (62.7 mg, 0.094 mmol) and hexamethylditin (46.3 mg, 0.14 mmol) in dry THF (3 ml) is added dichlorobis(triphenylphosphine)palladium (H) (2.0 mg, 0.003 mmol). The reaction mixture is degassed and is heated at reflux for 3 hours. The reaction mixture is cooled and filtered through a pad of celite. The filtrate is absorbed onto silica gel and purified on a Biotage 12M column with SIM using 2 % CH 3 OH in CH 2 C1 2 as the eluent to afford 28.6 mg (0.04 mmol, 43 %) of the stannane.1H-NMR (DMSO) δ: 8.25, 8.04, 8.00, 7.48, 7.42, 7.15, 7.10, 5.10, 4.73, 4.09, 3.71, 3.60, 3.40, 3.02, 2.96, 1.83, 0.33. Step 3. To a stined solution of the stannane prepared in Step 2 in dry acetonitrile is added a solution of 1M aqueous Na 125 I followed by chloramine-T hydrate. After stirring at room temperature for 30 minutes, the reaction mixture is quenched with saturated aqueous Na2S 2 O 3 and purified to give the radioiodinated material. Example 4: Synthesis N-[[(5S)-3-[4-[4-(4-Azido-3-iodo- 125 I-benzoyl)-l-piperazinyI]-3-fluorophenyl]-2-oxo-5- oxazolidinyl]methyl] acetamide (Compound D 3 of Scheme D where L is a bond, X is F, Y is H, Q is oxazolidinone, Z is O and R 4 is CH 3 ) Step 1. To a stirred solution of 4-azido-3-iodobenzoic acid (272.0 mg, 0.94 mmol) in dry THF (4 ml) is added 1,1-carbonyldiimidazole (152.6 mg, 0.94 mmol). The reaction mixture is stined at room temperature for 1 hour, then (S)-N-[[3-[4-[3-fluoro-4-(l-piperazinyl)]phenyl]- 2-oxo-5-oxazolidinyl]methyl]-acetamide (315.9 mg, 0.94 mmol) is added followed by DMF (2 ml). The reaction mixture is heated at reflux for 18 hours. The reaction mixture is cooled and poured into CH 2 C1 2 (40 ml) and successively washed with H 2 O (20 ml), 1 N HC1 (20 ml), saturated aqueous NaHCO 3 (20 ml), brine (20 ml), dried (MgSO 4 ), filtered and concentrated. The residue is dissolved in CH 2 CI 2 , absorbed onto silica gel and is purified on a Biotage 40S column with SIM using 2.5 % CH 3 OH in CH 2 C1 2 as the eluent to afford 376.7 mg (0.62 mmol) of the benzamide as a yellow solid. 1H-NMR (DMSO) δ: 8.24, 7.88, 7.53, 7.47, 7.39, 7.19, 7.08, 4.71, 4.08, 3.70, 3.51, 3.40, 2.99, 1.83. Step 2. To a stirred solution of the iodobenzamide prepared in Step 1 (82.4 mg, 0.13 mmol) and hexamethylditin (71.1 mg 0.22 mmol) in dry THF (6 ml) is added tetrakis(triphenylphosphine)palladium(0). The reaction mixture is degassed and heated at reflux for 12 hours. The cooled reaction mixture is filtered through a plug of celite and the filtrate is absorbed onto silica gel and purified on a Biotage 12M column with SIM using 2 % CH 3 OH in 49% CH 2 CI 2 and 49% EtOAC as the eluent to afford 28.2 mg (0.044 mmol, 34 %) of the stannane. 1H-NMR (DMSO) δ: 8.24, 7.50, 7.43, 7.35, 7.18, 7.17, 4.71, 4.08, 4.01, 3.70, 3.40, 2.99, 1.83, 0.32. Step 3. To a stirred solution of the stannane prepared in Step 2 in dry acetonitrile is added a solution of 1M aqueous Na 125 I followed by chloramine -T hydrate. After stirring at room temperature for 30 minutes, the reaction mixture is quenched with saturated aqueous Na 2 S 2 θ 3 and purified to give the desired radioiodinated material. Example 5: Synthesis N-[[(5S)-3-(4'-Azido-2-fluoro[l,l'-biphenyl]-4-yl)-2-oxo-5-oxazolidinyl]methyl]-T 3 - acetamide (Compound F 3 of Scheme F where R is H, R 2 is H, X is F, and Y is H) Step 1. To a stirred solution of 4-iodonitrobenzene (6.86 g, 27.5 mmol) in dry DMF (230 ml) is added bis(pinacolato)diboron (8.24 g, 32.4 mmol) followed by potassium acetate (8.68 g, 88.5 mmol) and [l, -bis(diphenylphosphino)fenocene]dichloropalladium(H) (624.6 mg, 0.76 mmol). The reaction mixture is degassed and heated at 85 °C for 2 hours. To the cooled dark reaction mixture is added (S)-N-[[3-(3-fluoro-4-iodophenyl)-2-oxo-5-oxazolidinyl]methyl] acetamide (5.8 g, 15.3 mmol) followed by 2 N aqueous Na 2 CO 3 (143 ml) and [ 1,1' -bis (diphenylphosphino)ferrocene]dichloropalladium(H) (312.0 mg, 0.38 mmol). The reaction mixture is degassed and heated at 85 °C for 3 hours. The cooled reaction mixture is partitioned between EtOAC (500 ml) and H 2 O (300 ml). The phases are separated. The aqueous layer is extracted with EtOAC (300 ml). The organic layers are combined and successively washed with H 2 O (500 ml), brine (500 ml), dried (MgSO 4 ), filtered and concentrated. The residue is dissolved in CH 3 OH/CH 2 CI 2 , absorbed onto silica gel and is purified on a Biotage 40 M column (2 lots) with SIM using 75 % EtOAC in CH 2 C1 2 to 100 % EtOAC as the eluent to afford 3.74 g (10.0 mmol, 65%) of the desired nitrobiphenyl compound. 1H-NMR (DMSO) δ: 8.30, 7.83, 7.68, 7.50, 4.78, 4.18, 3.80, 3.44, 1.84. Step 2. A mixture of the nitrobiphenyl compound prepared in Step 1 (3.74 g, 10.0 mmol), 10% palladium on carbon in THF (100 ml), CH 3 OH (100 ml) and CH 2 C1 2 (100 ml) is hydrogenated under a balloon of hydrogen for 18 hours. The reaction mixture is filtered through a pad of celite and the filtrate is concentrated to afford 2.50 g (7.3 mmol, 73%) of the desired aminobiphenyl. 1H-NMR (DMSO) δ: 8.26, 7.45, 7.34, 7.23, 6.64, 5.28, 4.73, 4.14, 3.76, 3.42, 1.84. Step 3. To a stirred solution of the aminobiphenyl prepared in Step 2 (508.93 mg, 1.48 mmol) in CH 3 OH (40 ml) and 1 M HC1 (40 ml), cooled to 0 °C is added a 1.2 M aqueous NaNO 2 solution (1.48 ml, 1.78 mmol). The reaction mixture is stirred at 0°C for 90 minutes, then sulfamic acid (143.5 mg, 1.48 mmol) is added followed by sodium azide (115.4 mg, 1.78 mmol) in H 2 O (1.5 ml). The reaction mixture is stined at 0 °C for 45 minutes, then diluted with CH 2 C1 2 (200 ml). The phases are separated. The aqueous phase is extracted with CH 2 CI 2 (75 ml). The combine organic phases are dried (MgSO 4 ), filtered and concentrated. The residue is dissolved in CH OH/CH 2 CI 2 , absorbed onto silica gel and is purified on a Biotage 40S column with SIM using 10% CH 3 OH in CH 2 C1 2 as the eluent to afford 262.9 mg (0.71 mmol, 48%) of the desired azidobiphenyl as a pale yellow solid. 1H-NMR (DMSO) δ: 8.27, 7.58, 7.42, 7.24, 4.76, 4.16, 3.78, 3.43, 1.84. Step 4. The azidobiphenyl prepared in Step 3 (102.4 mg, 0.27 mmol) in 6 N HC1 (2 ml) and CH 3 OH (6 ml) is heated at reflux for 18 hours. The CH 3 OH is removed in vacuo and the solid precipitate is isolated by filtration and is washed successively with H 2 O (10 ml), ether (2 x 15 ml) then dried to afford 82.1 mg (0.23 mmol, 82%) of the desired amine hydrochloride. 1H- NMR (DMSO) δ: 8.30, 7.62, 7.42, 7.25, 4.98, 4.25, 3.91. Step 5. To a stirring solution of 0.57 mg (6.94 μmol, 250 mCi) of tritiated acetic acid sodium salt (American Radiolabeled Chemicals, lot no ARC 990519) in 1 ml of dry DMF and 2.71 mg (21 μmol) of diisopropylethylamine at room temperature, is added 6.94 μmol of 0.45M O-Benzotriazol-l-yl-N,N,N',N'-tetramethyluronium hexafluorophosphate (HBTU) in dry DMF. The solution instantly turned pale yellow and is stined at room temperature for 10 minutes. The activated [ 3 H]acetic acid sodium salt was then added to a stirring solution of 2.73 mg (7.5 μmol) of the amine hydrochloride prepared in Step 4 in 2 ml of dry DMF. The reaction is stined at room temperature for 4.5 hours, then all solvents are removed by vacuum distillation at room temperature. The crude reaction mixture is purified on a preparative TLC plate (Analtech Silica gel GF, 500 micron, 20 cm x 20 cm plate), eluted with 8% methanol in dichloromethane. The desired band is scrapped. The product is eluted from the silica gel with 20% methanol in dichloromethane and filtered. The filtrate is concentrated under vacuum, and the residue is dissolved in 65.5 ml of methanol to afford 94.4 mCi of the desired tritiated material (1.44 mCi/ml methanol, specific activity 57.37 mCi/mg (57.37 Ci/mmol), radiochemical purity 99.5% by HPLC). Example 6: Synthesis N-[[(5S)-3-(4' -Azido-2-fluoro-3 ' -iodo[l, 1 ' -biphenyl]-4-yl)-2-oxo-5- oxazolidinyl]methyl]-T 3 -acetamide (Compound F 3 of Scheme F where R^s H, R 2 is I, X is F, and Y is H) Step 1. To a stirred solution of the aniline prepared in Step 2 of Example 5 (284.9 mg, 0.83 mmol) in acetic acid (3 ml) is added iodine monochloride (134.5 mg, 0.83 mmol) in acetic acid (0.25 ml). The reaction mixture is stined at room temperature for 1.5 hours. The reaction mixture is partitioned between EtOAc and aqueous Na 2 S 2 θ 3 . The phases are separated. The aqueous phase is extracted with EtOAc (20 ml). The combined organic phases were dried (MgSO 4 ), filtered and concentrated. The residue is dissolved in CH 3 OH, absorbed onto silica gel and is purified on a Biotage 40S column with SIM using 10-25 % acetone in CH 2 C1 2 as the eluent to afford 48.4 mg (0.10 mmol, 12%) of the desired iodoaniline as a yellow oil. 1H-NMR (CH 3 OD) δ: 7.73, 7.52, 7.29, 6.85, 4.81, 4.10, 3.79, 3.53, 3.32, 1.98. Step 2. To a stirred solution of the iodoaniline prepared in Step 1 (47. 2 mg, 0.10 mmol) in CH 3 OH (2 ml) and IN HC1 (2 ml) cooled to 0 °C, is added a solution of NaNO 2 (8.5 mg, 0.12 mmol) in H 2 O (1 ml). The yellow reaction mixture is stined at 0 °C for 30 minutes, then a solution of NaN 3 (8.0 mg, 0.12 mmol) in H 2 O (1 ml) is added. The reaction mixture is stirred at 0 °C for 1 hour, during which time a yellow precipitate formed. The solid is isolated by filtration and washed with H 2 O and dried to afford 41.0 mg (0.083 mmol, 83%) of the desired iodoazidobiphenyl as a yellow solid, mp 173-175 °C (dec). 1H-NMR (DMSO) δ: 8.27, 7.98, 7.60, 7.42, 4.76, 4.16, 3.78, 3.43, 1.84. Step 3. A mixture of 129 mg (0.26 mmol) of the iodoazidobiphenyl prepared in Step 2 (129.0 mg, 0.26 mmol), CH 3 OH (6 ml) and 1 N HC1 (2 ml) are heated at reflux for 48 hours. The cooled reaction mixture is concentrated to afford quantitative yield the desired amine hydrochloride as a tan solid. 1H-NMR (CH 3 OD) δ: 7.96, 7.63, 7.46, 7.38, 7.29, 5.04, 4.34, 3.93, 3.38, 1.30. Step 4. To a solution of 5.1 mg (0.05 mmol, 25 mCi) of tritiated acetic anhydride (Amersham Batch B77, isotope # 00-0316) is added 2 N PC1 3 in CH 2 C1 2 (25 μl). The reaction mixture is left at room temperature for 5 hours with occasional mixing. To this mixture is added a solution of the amine hydrochloride prepared in Step 3 (47.7 mg, 0.104 mmol) in pyridine (0.25 ml) followed by DMAP (4.6 mg). After 30 minutes, the reaction mixture is partitioned between H 2 O and CH 2 C1 2 . The phases are separated. The aqueous phase is extracted exhaustively with CH 2 C1 2 and then concentrated. The residue is purified on silica gel (4 g) using 20 % acetone in toluene as the eluent to afford 38.2 mg (0.077 mmol, 74 %) of desired tritiated acetamide. Example 7: Synthesis N-[[(5S)-3-(4'-Azido-2-fluoro-3'-iodo[l,l'-biphenyl]-4-yl)-2-oxo-5- oxazolidinyfjmethyl] ethane- 35 S-thioamide (Compound G 2 of Scheme G where RHs H, R 2 is I, X is F, and Y is H) Step 1. Methylmagnesium chloride in tetrahydrofuran (THF) is treated with S labeled carbon disulfide at 40 °C, followed by treatment with ethyl iodide. The reaction is stirred at 60 °C for 1.5 hours. After workup with water and ethyl ether, the desired ethyl 35 S-dithioacetate is obtained. Step 2. The amine hydrochloride salt prepared in Step 3 of Example 6 and the ethyl [ S]dithioacetate prepared in Step 1 are stined in methylene chloride, methanol, and triethylamine to give the desired S labeled thioamide. Example 8: Synthesis N-[[(5S)-3-(4'-Azido-2-fluoro-3'-iodo- 125 I-[l,l'-biphenyl]-4-yl)-2-oxo-5- oxazolidinyl]methyl] aacceettaammiide (Compound H 3 of Scheme H where X is F, Y is H, Q is oxazolidinone, Z is O, and R 4 is CH 3 ) Step 1. To a stined solution of the iodobiphenyl prepared in Step 2 of Example 6 (56.2 mg, 0.11 mmol) and hexamethylditin (73.9 mg, 0.22 mmol) in toluene (5 ml) is added palladium (H) acetate (2.6 mg, 0.011 mmol) followed by triphenylphosphine (6.5 mg, 0.022 mmol). The reaction mixture is degassed and heated at 80 °C for 20 hours. The cooled reaction mixture is concentrated to one half the volume, then purified on a Biotage 12S column using 10-20 % acetone in CH 2 C1 2 as the eluent to afford 53.5 mg (0.10 mmol, 89%) of the desired stannane. 1H- NMR (CDC1 3 ) δ: 7.53, 7.43, 7.30, 7.21, 6.07, 4.83, 4.11, 3.83, 3.73, 2.05, 0.35. Step 2. To a stined solution of the stannane prepared in Step 1 in dry CH 3 CN and pH 7 phosphate buffer is added chloramine-T followed by a solution of 1M aqueous Na 125 I. After 30 minutes, the reaction mixture is quenched with saturated aqueous Na 2 S 2 O 3 and purified to give the title compound. Example 9: Synthesis (2E)-3-(4-Azido-3-iodo- 125 I-phenyl)-N-[[(5S)-3-[3-fluoro-4-(4-pyridinyl)phenyl]-2-oxo- 5-oxazolidinyl]methyl]-2-propenamide (Compound L t of Scheme I where R is 4-pyridyl, X is F, Y is H, n is 1, R 1 is 125 I, and R 2 is H) Step 1. To a stined solution of oxalyl chloride (0.10 mL, 1.2 mmol) in CH 2 CI2 (1.5 ml), cooled to -78 °C, is added dry DMSO (0.14 ml, 1.97 mmol). After 10 minutes, a solution of 4- azido-3-iodobenzyl alcohol (217.0 mg, 0.79 mmol (Shu, 7. of Labeled Compounds and Radiopharmaceuticals, 1996, 38, 227-237)) in CH 2 CI 2 (2.5 ml) is added. After 15 minutes, triethylamine (0.33 ml, 2.37 mmol) is added and the reaction mixture is allowed to warm to room temperature. The reaction mixture is poured into CH 2 CI 2 (30 ml) and washed successively with H 2 O (20 ml), brine (20 ml), dried (MgSO ), filtered and concentrated. The residue is purified on a Biotage 12M column using 10% EtOAC in hexane to afford 195.5 mg (0.72 mmol, 91%) of the desired aldehyde. 1H-NMR (CDC1 3 ) δ: 9.9, 8.31, 7.93, 7.28. Step 2. To a stined solution of the aldehyde prepared in Step 1 (190.0 mg, 0.69 mmol) in dry THF (1 ml) is added triethylphosphonoacetate (0.15 ml, 0.76 mmol) followed by lithium hydroxide monohydrate (32.1 mg, 0.76 mmol). The reaction mixture is stirred at room temperature for 48 hours. The reaction mixture is poured into CH 2 C1 2 (40 ml) and successively washed with H 2 O (20 ml), brine (20 ml), dried (MgSO 4 ), filtered and concentrated. The residue is dissolved in CH 2 C1 2 , absorbed onto silica gel and purified on a Biotage 40S column with a SIM using 5% EtOAC in hexane as the eluent to afford 165.1 mg (0.48 mmol, 70 %) of the desired ester, mp 93-94 °C. 1H-NMR (CDC1 3 ) δ: 7.97, 7.56, 7.16, 6.40, 4.29, 1.35. Step 3. To a stined solution of the ester prepared in Step 2 (66.7 mg, 0.19 mmol) in CH 3 OH (2 ml) is added 1 N LiOH (0.19 ml, 0.19 mmol). The reaction mixture is heated at reflux for 12 hours. The cooled reaction mixture is concentrated and used immediately. Step 4. (S)-N-[[3-[3-fluoro-4-(4-pyridyl)phenyl]-2-oxo-5-oxazolidinyl]methyl] acetamide (1.40 g, 4.25 mmol) in CH 3 OH (62 ml) and 6 N HC1 (31 ml) is heated at reflux for 18 hours. The reaction mixture is concentrated to afford 1.48 g of the amine bis-hydrochloride salt. 1H-NMR (DMSO) δ: 8.98, 8.62, 8.26, 7.75, 7.56, 7.35, 5.76, 5.07, 4.28, 4.30, 3.27. Step 5. The amine bis-hydrochloride (from Step 4) (68.2 mg, 0.19 mmol), the lithium carboxylate prepared in Step 3, l-[3-(dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride (72.8 mg, 0.38 mmol) and 1-hydroxybenzotriazole hydrate (30.8 mg, 0.23 mmol) are dissolved in pyridine (2 ml) and stirred at room temperature for 72 hours. The reaction mixture is concentrated. The residue is dissolved in CH 2 CI 2 (40 ml) and washed with H 2 O (20 ml), brine (20 ml), dried (MgSO 4 ), filtered and concentrated. The residue is dissolved in CH 3 OH CH 2 Cl 2 , absorbed onto silica gel and is purified on a Biotage 12M column with SIM using 2 % CH 3 OH (saturated with NH 3 ) in CH 2 CI 2 as the eluent to afford 56.2 mg (0.096 mmol, 51%) of the desired cinnamide. 1H-NMR (DMSO) δ: 8.66, 8.48, 8.02, 7.64, 7.49, 7.40, 7.35, 6J0, 4.86, 4.22, 3.84, 3.60. Step 6. To a stined solution of the iodocinnamide prepared in Step 4 and hexamethylditin in dry THF is added tetrakis(triphenylphosphine)palladium(0). The reaction mixture is degassed and heated at reflux for 12 hours. The cooled reaction mixture is filtered through a plug of celite and the filtrate is absorbed onto silica gel and purified on a Biotage 12M column with SEVI to afford the stannane. Step 7. To a stined solution of the stannane prepared in Step 5 in dry acetonitrile is 1 added a solution of IM aqueous Na I followed by chloramine -T hydrate. After stirring at room temperature for 30 minutes, the reaction mixture is quenched with saturated aqueous Na 2 S 2 O 3 and purified to give the desired radioiodinated material. Example 10: Synthesis 4-Azido-N-[[(5S)-3-[3-fluoro-4-(4-pyridinyl)phenyl]-2-oxo-5-oxazolidinyl]methyl]-2- hydroxy-5-iodo- 125 I -benzamide (Compound J 2 of Scheme J where R 5 is 4-pyridyl, X is F, and Y is H, and n is 0) Step 1. To a stined suspension of the amine bis-hydrochloride salt prepared in Step 4 of Example 9 (172.4 mg, 0.48 mmol) in pyridine (4 ml) and CH 2 CI 2 (1 ml) is added 4-azidosalicylic acid (128.9 mg 0.72 mmol) followed by added l-[3-(dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride (184.0 mg, 0.96 mmol) and 1-hydroxybenzotriazole hydrate (77.8 mg, 0.58 mmol). The reaction mixture is stined at room temperature for 72 hours then concentrated. The residue is dissolved in CH 3 OH/CH 2 Cl 2 , absorbed onto silica gel and purified on a Biotage 40S column with SIM using EtOAC as the eluent to afford 44.8 mg (0.10 mmol, 21%) of the benzamide as a tan solid, mp 200-202 °C (dec). 1H-NMR (DMSO) δ: 12.5, 9.1, 8.66, 7.92, 7.70, 7.67, 7.61, 7.50, 7.46, 6.70, 6.60, 4.93, 4.25, 3.92, 3.70. Step 2. Starting with the phenol prepared in Step 1, 125 I is introduced according to the procedure described in Step 2 of Example 1. Example 11: Synthesis N-(4-Azidophenyl)-N'-[[(5S)-3-[3-fluoro-4-(4-pyridinyl)phenyl]-2-oxo-5-oxazolidinyl] methyl] - 35 S-thiourea (Compound L 3 of Scheme L where R 5 is 4-pyridyl, X is F, Y is H, R 1 is H and R is H) To a stirred solution of the amine bis-hydrochloride (from Step 4 of Example 9) in dry THF is added Hunig' s base followed by 35 S-4-azidophenylisothiocyanate in THF. The reaction mixture is heated at reflux for 1 hour. The cooled reaction mixture is cooled and purified to give the desired thiourea. As those skilled in the art will appreciate, numerous changes and modifications may be made to the preferred embodiments of the invention without departing from the spirit of the invention. It is intended that all such variations fall within the scope of the invention. The entire disclosure of each publication cited herein is hereby incorporated by reference.

Claims: What is claimed is: 1. A compound comprising the formula wherein: X and Y are, independently, F, H or CH 3 ; R 1 is H, F or I; R 2 is H, F or OH; R 16 isHorF; R 17 isHorF; R 3 is H or Cι-C 8 alkyl; L is a bond or -OCH 2 C(=O); and Qis wherein: R 4 is H, CH 3 , CH 2 CH 3 or cyclopropyl; and ZisOorS; or a pharmaceutically acceptable salt thereof. 2. A compound of claim 1 wherein X is F, Y is H, R 3 is H, and R 4 is CH 3 . 3. A compound of claim 1 wherein said compound is 2-[4-[4-[(5S)-5- [( Acetylamino)methyl] -2-oxo-3 -oxazolidinyl] -2-fluorophenyl] - 1 -piperazinyl] -2-oxoethyl-4- azido-2-hydroxy-5-iodo- 125 I τ -benzoate 4. A compound of claim 1 wherein said compound is N-[[(5S)-3-[4-[4-(4-Azido-2- hhyyddrrooxxyy--55--iiooddoo-- II--bbeennzzooyyll))--ll--piperazinyl]-3-fluorophenyl]-2-oxo- oxazolidinyl]methyl]acetamide. 5. A compound of claim 1 wherein said compound is 2-[4-[4-[(5S)-5- [(Acetylamino)methyl]-2-oxo-3-oxazolidinyl]-2-fluorophenyl]- 1 -piperazinyl] -2-oxoethyl 4- azido-3-iodo- 125 I-benzoate. 6. A compound of claim 1 wherein said compound is N-[[(5S)-3-[4-[4-(4-Azido-3-iodo- 125 I-benzoyl)-l-piperazinyl]-3-fluorophenyl]-2-oxo-5-oxazolidinyl]methyl]acetamide. 7. A compound comprising the formula wherein: X and Y are, independently, F, H or CH 3 ; R 1 is H, F or I; R 2 is H, F or OH; R , 1 1 6 0 is H or F; R 17 is H or F; and Q is R 4 is H, CH 3 , CH 2 CH 3 or cyclopropyl; and Z is O or S; or a pharmaceutically acceptable salt thereof. 8. A compound of claim 7 wherein X is F, Y is H, and R 4 is CH 3 . 9. A compound of claim 7 wherein said compound is N-[[(5S)-3-(4'-Azido-2-fluoro[l,l '- biphenyl]-4-yl)-2-oxo-5-oxazolidinyl]methyl]-T 3 -acetamide. 10. A compound of claim 7 wherein said compound is N-[[(5S)-3-(4'-Azido-2-fluoro-3'- iodo[l, -biphenyl]-4-yl)-2-oxo-5-oxazolidinyl]methyl]-T 3 -acetamide. 11. A compound of claim 7 wherein said compound is N-[[(5S)-3-(4' -Azido-2-fluoro-3 ' - iodo[l,r-biphenyl]-4-yl)-2-oxo-5-oxazolidinyl]methyl]ethane- 35 S-thioamide. 12. A compound of claim 7 wherein said compound is N-[[(5S)-3-(4'-Azido-2-fluoro-3'- iodo- 125 I-[l,l'-biphenyl]-4-yl)-2-oxo-5-oxazolidinyl]methyl]acetamide. 13. A compound comprising the formula Y wherein: X and Y are, independently, F, H or CH 3 ; R 5 is wherein: R 6 is H, N 3 , halogen, NH 2 , OH, SH, C 1 -C 4 alkylamino, C 1 -C 4 dialkylamino, CrC 4 alkyl, nitrile, carboxamide, C 1 -C 4 alkoxy, -C 4 alkylthio, or C 1 -C 4 alkoxycarbonyl; and Pis wherein: Zis O or S; and R 7 is wherein: R 1 is H, For I; R .2 i •s H, F or OH; R ,1 i 6 D is H or F; and R ,1A7.sHorF; or a pharmaceutically acceptable salt thereof. 14. A compound of claim 13 wherein X is F, Y is H, and R is H. 15. A compound of claim 13 wherein said compound is (2E)-3-(4-azido-3-iodo- 12 5τ I- phenyI)-N-[[(5S)-3-[3-fluoro-4-(4-pyridinyl)phenyl]-2-oxo-5-oxazolidinyl]methyl]-2- propenamide. 16. A compound of claim 13 wherein said compound is 4-azido-N-[[(5S)-3-[3-fluoro-4-(4- pyridinyl)phenyl]-2-oxo-5-oxazolidinyl]methyl]-2-hydroxy-5-iodo- I-benzamide. 17. A compound of claim 13 wherein said compound is N-(4-azidophenyl)-N'-[[(5S)-3-[3- fluoro-4-(4-pyridinyl)phenyl]-2-oxo-5-oxazolidinyl]methyl]- 35 S-thiourea. 18. A method of using a compound comprising the formula wherein: X and Y are, independently, F, H or CH 3 ; R 8 is H, F or I; R 9 is H, F or OH; R 18 is H or F; R 19 is H or F; R 10 is H or Cj-Cs alkyl; L is a bond or -OCH C(=O); and Q is wherein: R »π 11 is H, CH 3 , CH 2 CH 3 or cyclopropyl; and Z is O or S; or a pharmaceutically acceptable salt thereof, as a photoaffinity probe. 19. The method of claim 18 comprising the steps: contacting a cell or component thereof with said compound, wherein said compound is radiolabeled; exposing said radiolabeled compound to light; and detecting said radiolabel. 20. The method of claim 19 further comprising contacting said cell or components thereof with a competitor compound. 21. A method of using a compound comprising the formula Y wherein: X and Y are, independently, F, H or CH 3 ; R 12 is N 3 or wherein: R 8 is H, For I; R 9 is H, F or OH; R .1 1 8 0 isHorF;and R ,1A9 sHorF;and Qis wherein: R 11 is H, CH 3 , CH 2 CH 3 or cyclopropyl; and ZisOorS; or a pharmaceutically acceptable salt thereof, as a photoaffinity probe. 22. The method of claim 21 comprising the steps: contacting a cell or component thereof with said compound, wherein said compound is radiolabeled; exposing said radiolabeled compound to light; and detecting said radiolabel. 23. The method of claim 22 further comprising contacting said cell or components thereof with a competitor compound. 24. A method of using a compound comprising the formula wherein: X and Y are, independently, F, H or CH 3 ; R 13 is wherein: R , 1 1 4 4 is H, N 3 , halogen, NH 2 , OH, SH, Cι-C 4 alkylamino, C C 4 dialkylamino, - alkyl, nitrile, carboxamide, Cι-C 4 alkoxy, C 1 -C 4 alkylthio, or C 1 -C4 alkoxycarbonyl; and P is wherein: Z is O or S; and wherein: R 8 is H, F or I; ' R 9 is H, F or OH; R 18 is H or F; and R 19 is H or F; or a pharmaceutically acceptable salt thereof, as a photoaffinity probe. 25. The method of claim 24 comprising the steps: contacting a cell or component thereof with said compound, wherein said compound is radiolabeled; exposing said radiolabeled compound to light; and detecting said radiolabel. 26. The method of claim 25 further comprising contacting said cell or components thereof with a competitor compound.

Inventors: GADWOOD ROBERT C, THOMASCO LISA MARIE, GADWOOD, ROBERT, C., THOMASCO, LISA, MARIE
Applicants: GADWOOD ROBERT C, THOMASCO LISA MARIE, UPJOHN CO, GADWOOD, ROBERT, C., PHARMACIA & UPJOHN COMPANY, THOMASCO, LISA, MARIE
Application year: 2001
Main classification: C07D413
IPC codes: A61P31, C07D263, C07D401, C07D413, G01N33