HIGH AFFINITY INTEGRIN POLYPEPTIDES AND USES THEREOF

Abstract: Polypeptides comprising all or part of a variant integrin alpha subunit A domain or a variant integrin beta subunit A-like domain are described.

Description: HIGH AFFINITY INTEGRIN POLYPEPTIDES AND USES THEREOF RELATED APPLICATION INFORMATION This application claims priority from provisional application serial no. 60/221,950, filed July 31, 2000, and from U.S. application serial number 09/758,493, filed January 11, 2001, the contents of which are hereby incorporated by reference. BACKGROUND Integrins are heterodimeric receptors that mediate a wide variety of important interactions both between cells and between cells and the extracellular matrix via ligand binding. All integrins have an α subunit and a β subunit. Within the α subunit a region referred to as the A domain (or I domain) is known to be an important mediator of ligand binding. A similar region, the A-like domain, is present in many β subunits. Many integrins are thought to exist in two conformations, a low affinity state (the "closed" or "unliganded" conformation") and a high affinity state (the "open" or "Iiganded" conformation), the later of which is responsible for high affinity ligand binding. Integrins transduce signals that mediate the effects of the matrix on the physiological activity of cells (e.g., motility, proliferation, and differentiation). Moreover, integrins play a role in inflammation and in oncogenic cell transformation, metastasis, and apoptosis. Thus, there is considerable interest in identifying compounds that can activate or inhibit the activity of one or more integrins. In order for an efficient integrin-ligand binding to occur, it is thought that the integrin must be in its high affinity configuration. It appears that inside-out signals generated when cells are activated by a variety of stimuli switch integrins from a low affinity state to a high affinity state. This functional upregulation is associated with conformational changes in the extracellular regions of integrins that include the A domain of the α subunit and the A-like domain of the β subunit (Smith et al. 1988 J. Biol. Chem. 263 : 18726). The integrin A-domain assumes a dinocleotide-binding fold (Lee et al. 1995 Cell 80:631; Emsley et al. 1997 J Biol. Chem..272:28512; Li et al. 1998 J. Cell Biol. 143:1523; Nolte et al. 1999 FEBSLett. 452:379; and Legge et al. 2000 J. Mol. Biol. 295:1251) with a metal ion dependent adhesion site (MJJDAS) on its top, and it is connected through a C- terminal α7 helix at its bottom to the body of the integrin. MIDAS and its surrounding exposed side-chains form the binding site for physiologic ligands (Li et al., supra; Michishita et al., Cell 72:857-867, 1993; Kamata et al., J. Biol. Chem. 269:26006-26010, 1994; Kern et al., J. Biol. Chem. 269:22811-6, 1994; Edwards et al., JBiol Chem. 273:28937-44, 1998; Zhang et al., Biochemistiy 38:8064-71, 1999) and certain antagonists (Rieu et al., JBiol Chem. 271:15858-15861, 1996). In the "open" conformation, three non-charged resides in the protein directly coordinate the metal ion in MIDAS. A pseudoligand or ligand glutamate residue (Lee et al., supra; Li et al, supra; Emsley et al., Cell 100:47-56, 2000) completes metal coordination. In the "closed" form, the amphipathic C-terminal α 7 helix is shifted upwards by 10 A compared to the "open" form, wrapping around the rest of the domain. This large shift is associated with a change in metal coordination, where one of the three coordinating residues, a threonine, is now replaced with an aspartate, and a water molecule replaces the glutamate in completing the metal ion coordination sphere (Lee et al, Structure 3:1333-1340, 1995). These changes in metal coordination and topology of MIDAS are similar to those described in the structurally homologous G proteins (Lee et al, supra). The crystal structure of four integrin A-domains (CDl lb, CDl la, CD49a and CD49b) have been reported to date (Lee et al, Cell 80:631, 1995; Lee et al, Structure 3:1333, 1995; Emsley et al, supra; Li et al, supra; Emsley et al, JBiol Chem 272:28512, 1997). All with the exception of integrin CDl lb A-domain (1 IbA), were found only in the "closed" form, leading to the suggestion that the "open" form is a non-informative crystal artifact (Baldwin et al, Structure 6:923-935, 1998). Three studies support the view that the "open" form of the integrin A-domain equates with the "high" affinity state (Li et al 1998 J. Cell Biol 143:1523; Rieu et al, supra; Oxvig et al, ProcNat'l. AcadSci USA 96:2215-20, 1999). In the first study, point mutations in CDl IbA that are predicted on structural grounds to destabilize the "closed" structure, increased the proportion of the "high affinity" form in solution (Li et al. 1998 J. Cell Biol 143:1523). In the second study, the binding site for an "activation-dependent" monoclonal antibody mapped to a conformationally sensitive region of the A-domain (Oxvig et al, supra). The third study showed that an A-domain in complex with a short collagen peptide assumed the "open" conformation, and suggested that the "open" form can only be obtained in the presence of ligand (Emsley et al, supra). While it has been suggested that the ligand causes the conformational change in integrins, at least one study suggests that integrins can exist in high affinity state even in the absence of ligand (Smith and Cheresh 1988 J. Bio.l Chem. 263:18726-31). In addition, several studies suggested that ligand binding affinity in heterodimeric integrins can be altered in an allosteric manner (Li et al. 1998 J Cell Biol 143:1523; Edwards et al, JBiol Chem 273:28937-28944, 1998; Calderwood et al, JBiol Chem 273:5625, 1998; Zhang et al, JBiol Chem. 271: 29953-7, 1996). SUMMARY The invention features polypeptides comprising all or part of a variant integrin α subunit A domain or a variant integrin β subunit A-like domain. In the polypeptides of the invention, referred to as variant integrin polypeptides, an important isoleucine (He) residue (described in greater detail below) is absent. The isoleucine can be either deleted or replaced with different amino acids residue, preferably a smaller or less hydrophobic amino acid residue, e.g., alanine or glycine. Because the variant integrin polypeptides of the invention tend to exist in solution in a high affinity conformation, they are useful in screening assays for the identification of molecules that bind to (and/or modulate the activity of) an integrin. They are also useful for generating antibodies, e.g., monoclonal antibodies, which selectively bind to the high affinity form of an integrin. Some such antibodies recognize an epitope that is either not present or not accessible on an integrin that is in a lower affinity conformation. Thus, the invention features antibodies which bind with greater affinity to a variant integerin of the invention than to corresponding wild-type integrin. The variant integrin polypeptides of the invention can be derived from any integrin α subunit or any integrin β subunit. The variant integrin polypeptides preferably include a ligand-binding portion of an A-domain or an A-like domain. The invention also features methods for identifying a compound that binds to a variant integrin polypeptide of the invention. Such screening methods can entail exposing the polypeptide to a test compound of interest and determining whether the compound binds to the polypeptide. Thus, the assay can be a simple binding assay (e.g., where binding of the compound is measured only in the absence of the ligand) or a competitive binding assay (e.g., where binding of the compound is measured in the presence of the ligand). The invention also features methods for identifying a compound that interferes with the binding of an integrin ligand to an integrin by measuring the binding of an integrin ligand to a variant integrin polypeptide in the presence and absence of a test compound. The ability of a test compound to interfere with the binding of an integrin ligand to an integrin may also be tested. In addition, the binding specificity of a compound can be assessed by measuring the binding of the integrin ligand to a second integrin (or measuring the binding of a second ligand to the integrin) in the presence and absence of the test compound. The invention also features methods for interfering with the binding of an integrin to an integrin ligand by administering a variant integrin polypeptide of the invention or an antibody that selectively binds to a variant integrin polypeptide of the invention. The invention also features methods of administering a variant integrin polypeptide of the invention or an antibody that selectively binds to a variant integrin polypeptide of the invention for the purpose of identifying the presence of a ligand which binds to an active integrin. Such assays are useful for diagnosing inflammation, e.g., occult inflammation (e.g. an abscess or an active atherosclerotic lesion). Compounds that bind to a variant A domain or A-like domain of the invention (e.g., compounds that bind relatively selectively to an A domain of A-like domain in the active conformation) are candidate compounds for the treatment of inflammatory disorders mediated by an integrin, e.g., a disorder mediated by CDl lb, CDl la, or CDl lc. The invention further features nucleic acid molecules (e.g., mRNA and DNA) encoding a polypeptide of the invention or a polypeptide which includes a polypeptide of the invention. The invention also includes nucleic acid molecule encoding a fusion polypeptide comprising a polypeptide of the invention and a second polypeptide, e.g., an immunoglobulin constant domain. The experiments described below concern the design and preparation of a variant form of CDl lb (an integrin α subunit) that is more active than the wild-type form of CDl lb. Without being bound by any particular theory, it appears that, in solution, the fraction of this variant subunit that is in the open (active) conformation is greater than for the corresponding wild-type form of the subunit. Creation of the variant CDl lb involves deletion or substitution of an invariable He residue. Deletion or substitution of the He residue confers a high affinity phenotype in isolated CDl lb A domain as well as in the intact CDl lb integrin. Moreover, the He- modified A-domain can be crystallized in the "open" conformation. Thus, without being bound by any particular theory, it appears that an lie-based allosteric switch controls affinity and conformation in the integrin A-domain. Accordingly, variant, high affinity forms of other integrin α subunits (and β subunits) can be created by deleting or substituting the corresponding highly conserved He residue. The details of one or more embodiments of the invention are set forth in the accompanying drawings and the description below. Other features, objects, and advantages of the invention will be apparent from the description and drawings, and from the claims. DESCRIPTION OF DRAWINGS Figures 1A and IB depict a surface representation of the CDl IbA domain crystal structure in its "closed" (Figure 1 A) and "open" (Figure IB) states, with the C-terminal α7 helix outlined as a gray ribbon, the isoleucine (1332) residue in black. An arrow points to the MIDAS face. MIDAS and SILEN (socket for isoleucine) lie on almost opposite ends of the A-domain structure. Note that the amino acids in these figures are number based on the number of the mature protein. The CDllb α subunit has a 16 amino acid signal sequence. Thus, 1332 in the immature protein corresponds to 1316 in the mature protein. Figures IC and ID depict magnified face views of SELEN residues (1151, L180, 1252, Y283, lying within 4A radius from 1332) in the "closed" (Figure IC) and "open" (Figure ID) conformations. In the "closed" form (Figure IC), 1332 sidechain is wedged in SILEN, and L328 is seen on top. L328 moves to cover SILEN in the "open" structure (Figure ID). Figures 1 A- ID were built using GRASP (Barry Honig, Columbia University, NY). Note that the amino acids in these figures are number based on the number of the mature protein. The CDllb α subunit has a 16 amino acid signal sequence. Thus, 1332 in the immature protein corresponds to 1316 in the mature protein. Figures IE and IF depict stereo views of the 1332 coordination socket in the "closed" (Figure IE) and "open" (Figure IF) conformations. SILEN residues and 1332 and L328 are labeled. Note that the amino acids in these figures are number based on the number of the mature protein. The CDllb α subunit has a 16 amino acid signal sequence. Thus, 1332 in the immature protein corresponds to 1316 in the mature protein. Figures 2 A and 2B depict 2F 0 -Fc electron density maps of the C-terminal portions of α7 from 1 IbA " (Figure 2 A) and lib A ' structures. The maps were contoured at 1. lσ, and made with O. Note that the amino acids in these figures are number based on the number of the mature protein. The CDllb α subunit has a 16 amino acid signal sequence. Thus, 1332 in the immature protein corresponds to 1316 in the mature protein. Figures 2C and 2D depict ribbon diagrams showing the complete superimposition of the llbA 139"337 (Figure 2C) and llbA 139'331 (Figure 2D) structures on the reported "closed" (Figure 2C) and "open" (Figure 2D) forms of the CDllb A domain, respectively. The metal ion is shown as a sphere. Note that the amino acids in these figures are number based on the number of the mature protein. The CDllb α subunit has a 16 amino acid signal sequence. Thus, 1332 in the immature protein corresponds to 1316 in the mature protein. Figures 2E and 2F depict stereo views of MED AS in the llbA 139"337 (Figure 2E) and llbA 139'331 (Figure 2F) structures. The direct D258-metal bond, and indirect T225-water- metal bond (Figure 2E) are characteristic of the "closed" conformation. The metal ion in this case is Mn 2+ . The MIDAS conformation (a direct T225-metal bond, indirect D258-water- metal bond, and a pseudo ligand glutamate, occupying the active site, shown in green, and directly coordinating the metal ion (Ca 2+ ) are those of the "open" conformation). Note that the amino acids in these figures are number based on the number of the mature protein. The CDllb α subunit has a 16 amino acid signal sequence. Thus, 1332 in the immature protein corresponds to 1316 in the mature protein. Figures 3A-3J depict the results of functional analysis of llbA E"G and llbA 139"331 A- domains using BIAcore™ for recording the interactions of llbA E"G (Figures 3A-3E) and llbA 139"331 (Figures 3F-3J) with the activation-dependent ligands iC3b (Figures 3 A and 3F), fibrinogen (Figures 3B and 3G) or CD54 (Figures 3C and 3H). All three ligands bound to 1 IbA 139"331 , but not to 1 IbA 139"337 . The observed differences in binding were not due to differences in protein concentration, as binding to the activation-independent ligands, NTF (Figures 3D and 31) and mAb 904 (Figures 3E and 3 J) were comparable. To quantitatively determine the affinity, various concentrations of the A domain peptides were used. The binding data were analyzed by linear transformation, giving dissociation constants (Kd, mean±SD, n=2) of 0.46±0.15 μM (for iC3b), 0.25±0.07 μM (for fibrinogen); and 0.22±0.04 μM (for CD54). Figure 4 A depicts the results of an analysis of the binding of llbA 1→G to iC3b. The dotted line represents the lack of binding of llbA to the same ligand. The calculated Kd is 0.66±0.3 μM (mean ± SD, n=2). Figure 4B depicts a histogram (mean ± SD, n=3) showing the relative binding of 1→G CR3 expressed on COS cells to iC3b, compared with wild-type receptor. Figure 5 depicts an alignment of the A domains of nine alpha integrin α subunit (CDllb, CDllc, CDlld, CDlla, alpha 1, alpha 2, alpha 10, alpha 11, and alpha E). In this alignment, the invariant He (1332 in CDllb α subunit) is indicated by an arrow. Figure 6 shows that an I to G mutation of the invariant isoleucine in CDlla A domain induces an activated domain in this integrin, underscoring the applicability of the findings in the CDllb A domain to other integrin A-domains. Figure 7 is an alignment of the A-like domains of eight integrin β subunits. In this alignment, the residue corresponding to the invariant He in β subunits is indicated by an arrow. DETAILED DESCRIPTION Example 1: Generation of a stable, high affinity CDl lb variant A-domain by deletion A variant CDl lb A domain consisting of amino acids E 139 to K 331 of CDl lb (1 IbA 139" 331 ; also referred to as 1 lbA E'K ) was created. (Note that amino acid numbering for the CDllb α domain is for the immature protein, including the 16 amino acid signal sequence). This variant A domain was characterized and compared to a CDl lb A domain consisting of amino acids E 139 to G 337 (1 IbA 139"337 ; also referred to as 1 lbA E" °). A variant A domain with a He to Gly change at residue 332 (1 lbA I→G ) was also created. All three proteins were expressed as GST fusion proteins that were cleaved to release the protein of interest. The variant polypeptides were created using standard recombinant techniques. Restriction and modification enzymes were purchased from New England Biolabs, Inc. (Beverly, MA), Boehringer Mannheim (Germany), or GTBCO BRL (Gaithersburd, MD). Site-directed mutagenesis was carried out in pGEX-4T-l vector as described (Rieu et al. 1996 JBiol Chem 271 : 15858). The following mutagenic primers were used. IFAdel Fwd: 5'-TATAGGATCCGAGGCCCTCCGAGGGAGTCCTCAAGAGGATAG-3'; Reverse: 5'- CTACTCGAGTTACTTCTCCCGAAGCTGGTTCTGAATGGTC-3'; I-G reverse: 5'- CTACTCGAGTTAACCCTCGATCGCAAAGCCCTTCTC-3'. Introduction of the respective mutation was confirmed by direct DNA sequencing. The PvuI-BspEI-restricted cDNA fragment of the A-domain containing the mutation was subcloned into the Pvul- BspEI-restricted CDllb cDNA, cloned into pcDNA3 plasmid, which containing full-length human CDl lb (Rieu et al 1996 JBiol Chem 271: 15858). l ib A 139"337 and llbA 139"331 and 1 lbA 1→G A-domains were expressed as GST fusion proteins in Escherichia coli (Michishita et al. 1993 Cell 72:857), cleaved with thrombin and purified as described Li et al. 1999 J. Cell Biol 143:1523. C 145 was replaced by S in all the expressed GST- A-domain fusion form to prevent formation of disulfide-linked dimmers in solution after thrombin cleavage (not shown). Purity was confirmed by SDS-PAGE analysis. The structures of 1 IbA 139"337 and 1 IbA 139"331 were determined by x-ray crystallography. Crystals were grown using lOmg/ml stock protein solutions and the hanging drop vapor diffusion method as described Li et al. (1998 J. Cell Biol 143:1523). Several crystal conditions were tried for each A-domain. 1 IbA 139"331 and 1 lbA I→G formed crystals in the presence of a reservoir solution containing 15% polyethylene glycol 8 K, 0.10 M Tris, pH 8.2, 150 mM CaCl 2 , at room temperature. Crystals started to form within a week, grew to a typical size of 0.3 mm x 0.05 mm x 0.04 mm in two weeks, and belonged to the tetragonal space group P4, with unit cell of a=b=45.7A. llbA " did not crystallize under these conditions, but formed crystals at room temperature using 10% polyethylene glycol 4000, 0.1M sodium citrate, pH 4.5, 5 mM MnCl 2 in the reservoir buffer. llbA " was crystallized in the P2 1 2 1 2 1 space group with unit cell of a =48.1 A, b = 121.5 A, c = 74.5 A. A single llbA " crystal was used to collect a 2.3 A resolution data set, at 100 K, on beamline XI 2B of the National Synchrotron Light Source at the Brookhaven National Laboratory using a CCD detector. A single 1 IbA " crystal was used to collect a 2.6A resolution data set, at 100K, using in-house X-ray on image plate. DATA were processed with DENZO and SCALEPACK to an Rsym of 8.8%, 7.7%, respectively. The starting models were the refined 1.8 AMg + structure(pdb accession code lido) (Lee 1995 Cell 80:631) comprising residues D148 to K331, and the refined 2.0 A Mg 2+ -structure (pdb accession code ljlm) (Lee et al 1998 Structure 6:923), comprising residues D 148 to A324, with the metal and water molecules removed, respectively. The preliminary rigid body refinements were performed using X-plor, with the diffraction data range from 8.0 to 3.0 A resolution with 5% of reflections for R-free calculation. The phases were gradually extended to high resolution and the structures were refined by several cycles of alternating torsion- angle dynamics and restrained individual isotropic B factor refinement protocols with all diffraction data between 8.0 and 2.6 A, and 8.0 and 2.3 A resolution, for llbA 139"337 and llbA " structures, respectively (Table 1). Model inspection and manual adjustments were made on SGI graphics workstations using O (Jones et al. 1991 Ada Crystallogr 47:110). The addition of solvent molecules was based on the suitable peaks in difference maps, reasonable hydrogen bond and refined temperature factors of less than 50 A 2 . The addition of solvent molecules was based on the suitable peaks in difference maps, reasonable hydrogen bond and refined temperature factors of less than 50 A 2 . The structure was refined to a final R factor of 20.3% (R-free: 25.6%) for the llbA 139"321 structure, and 21.9% (R-free: 30.0%) for the llbA " structure. The final models comprises all nonhydrogen atoms of residues D148 to K331, 30 water molecules, and one Ca 2+ ion for llbA 139"331 , and residues D148 to G337, 44 water molecules and one Mn 2+ ions for llbA 139"337 . Crystallographic data is presented in Table 1. Table 1 llbA 139-337 llbA 139-331 llbA I332→G Space group P2ι 2ι 2 P43 P43 Unit cell constants (A) a=48.1, b=121.5, a=b=45.7 a=b=45.2, c=74.6 c=94.8 c=95.0 No. of unique reflections 13,990 7,955 3,749 Resolution (A) 8.0 - 2.6 8.0 - 2.3 8.0 - 3.0 Rmerge ( )* 7.7 {23} § 8.8 {28} § 11.2 {30.4} § Completeness (%) 99.3 {98.4} § 91.1{80.8} § 95.3 {73.6}- Redundancy 5.9 2.3 2.7 R-factor ' (%) 21.9 18.8 20.8 R-free * (%) 30.0 24.8 28.8 Solvent molecules 82 62 20 Metal ions 2 (Mn) KCa) KCa) Root mean square (rms) deviations from ideal values Bond lengths (A) 0.007 0.007 0.006 Bond angle (°) 1.3 1.3 1.25 *Rmerge =∑|I-<I>|/∑ I where I is the observed intensity and <I> is the average intensity from multiple observations of symmetry-related reflections. * Numbers in {} shows values for highest 0.1 A resolution bin. 1 R-factor= ∑| Fo-Fc|Σ Fo. * R-free = ∑ τ | Fo-Fc|∑ τ Fo, where T is a test set containing a randomly selected 5% of the reflections omitted from the refinement. The crystal structure llbA " was that of the "closed" conformation (Figures 2A, 2C, and 2E). In contrast, llbA 139"331 crystallized in the "open" form (Figures 2B, 2D, and 2F). The ligand binding properties of llbA " and 1 IbA " were determined using surface plasmon resonance as previously described (Li et al, supra) on a BIAcore (BIAcore AB, Uppsala, Sweden). IC3b, fibrinogen or CD54 each was covalently couples via primary amine groups to the dextran matrix of a separate CM5 sensor chip (BIAcore AB). BSA immobilized in the same way was used as a control surface. 1 IbA " , 1 IbA ' and 1 lbA I→G A-domains were flowed over the chip at 5 ml/min at different times. TBS (20 mM Tris-HCl, pH 8.0, 150 mM NaCl) with 2 mM MgC 1 2 and 0.005% P20 (BIAcore AB) was used as the running buffer. 1 M NaCl in 20 mM Tris-HCl, pH 8.0, was used to remove the bound proteins and to regenerate the surface. Binding was measured as a function of time. The binding data (after subtracting background binding to BSA-coated chip) were analyzed using Scatchard plots as described (Dall'Acqua et al. 1996 Biochemstiy 35:9667-9676). COS M7 simian fibroblastoid cells at -70% confluence were transfected with supercoiled cDNAs encoding WT or CDl lb I→G together with full-length CD18 as described (Michishita et al, supra) Transfected COS cells were grown for 24 h in Iscove's modified Dulbecco's medium (BioWhittaker, Inc., Walkersville, MD) supplemented with 10% FBS, 2 mM glutamine, 50 IU/ml penicillin and streptomycin at 37°C. Cells were washed, detached with 0.1% trypsin- EDTA, and seeded in replicates for 24 h onto 24- or 48-well plates (Costar Corp., Cambridge, MA) or 100-mm petri dishes. Confluent monolayers in 24- or 48-well plates were used for cell-surface antigen quantification and ligand-binding studies, and those on petri dishes for immunoprecipitation studies. Heterodimer formation and binding of iC3b- coated erythrocytes to wild type and CR3 I→G holoreceptors were carried out as described (Rieu et al 1996 JBiol Chem 271 : 15858). Specific binding of iC3b to the holoreceptors was obtained by subtracting background binding to mock-transfected COS cells. Binding to CR3 →G was expressed as a percentage of binding to wild type, after correcting for the degree of surface expression using binding of mAb904 (Rieu et al. 1996 JBiol Chem 271:15858). As shown in Figures 3 A-3 J, 1 IbA " showed no binding to the activation- dependent "physiologic" ligands, complement iC3b, fϊrbrinogen and CD54 (ICAM-1). In contrast, llbA 139"331 displayed high affinity binding to all three ligands (Figures 3A-3J). Both domains bound equally well to the activation-independent "ligands" NIF (neutrophil inhibitory factor) (Figures 3D, and 31), and mAb 904 (Figures 3E and 3 J), indicating that the differences observed are not caused by variations in A-domain concentrations. These data conclusively establish that the "open" and "closed" crystal structures correspond respectively to the "high" and "low" affinity states of integrin 1 IbA. Example 2: Generation of a stable, high affinity state CDllb variant by substitution He 332 is invariable in all integrin alpha A-domains cloned to date (Figure 5). An A- domain with an He to glycine substitution (1 lbA I→G ) exhibited a "high affinity" state (Figure 4A). The same substitution created in the holoreceptor dramatically increased its ligand binding activity (Figure 4B). The crystal structure 1 lbA I→G was identical to that of the "open" 1 IbA 139"331 form. These data firmly establish that the "open" high affinity conformation is primarily dictated by an He-based switch, intrinsic to the domain, and acting allosterically to regulate ligand binding affinity on the MED AS face. It is known that the inactive an active conformers of an integrin exist in the absence of ligand (Smith et al, supra; Yan et al, J. Biol. Chem. 275:7249-60, 2000). Based on the results described above, it appears that the role of the ligand is not in initiating the high affinity state as has been recently proposed (Emsely et al, supra), but in stabilizing it, through shifting the equilibrium between the low and high affinity state in favor of the latter (Li et al, supra). Integrin activation by inside- out signaling would lead to a shift in this equilibrium, increasing the proportion of high affinity receptors on the cell surface that become "available" for ligand binding. Ligand engagement would then generate new epitopes, perhaps extrinsic to the A- domain, that initiate outside-in signaling. A conserved hydrophobic intramolecular socket (SILEN, Socket for Isoleucine), fastens the I finger in the "closed" conformation; I is replaced by L in the "open" structure (Figures 1 A and IF). SILEN is formed by the hydrophobic side chains of I 151 , L 180 , I 252 , and Y 283 both in the "closed" and "open" conformations. (Figures IC and ID). In several integrins, certain mutations that lie outside MIDAS produce gain-of function effects in the holoreceptor, and are believed to act allosterically (Zhang et al, supra; Oxvig et al, supra; Zhang et al., supra). These studies were carried out in the holoreceptors, making it difficult to provide a mechanistic interpretation, because of potential interdomain interactions and/or other quaternary effects. These mutations occur in or around SILEN. For example, substitution of the αl-βB loop of CDl lb with that of CDl la, generates a constitutively active integrin (Zhang et al, supra). This region spans one of the SILEN residues L 180 . Integrin activation also occurs in an L 180 -F substitution (Oxvig et al, supra), which predictably makes SILEN smaller and therefore less accommodation to I 332 . Other activating mutations involving E 147 , D 148 , K 247 and F 250 lie at the bottom of the structure, in close proximity to SILEN (Oxvig et al., supra), and may thus exert their effect through interference with the proper coordination of the He "finger" in SILEN. The inhibitory effect of certain mAbs with epitopes on the opposite side of MIDAS (e.g., mAb 44a, the epitope of which spans residues on the top of SILEN) may similarly be explained through stabilization of the STLEN pocket. The presence of the N-terminal extension facilitates A-domain switching into the less favored high-affinity state (Li et al, supra). The underlying structural basis for this effect is unknown, since none of the residues in the N-terminal extension are included in the derived 3-D structures. It has been observed however that residues within this extension regulate ligand binding in A-domains. First, naturally occurring point mutations in this segment of the vWF Al domain cause gain-of-function phenotypes in patients with type IDS vWf disease (Matsushita et al, J. Biol Chem 279:13406-14, 1995). This region also contains an activating mutation in CDl lb A domain (Oxvig et al, supra). Third, synthetic peptide from the N-terminal extension inhibited CD 11 a-dependent adhesion. Structural data from the CD49b A-domain also show that three residues that extend beyond the α7 helix can pack into a crevice formed in part by residues in the N-terminal extension, bringing the N and C termini into very close proximity (Emsley et al, supra). Flexibility of the C-terminal residues in α7 has also been observed in the crystal and NMR structures of the CDl la A- domain. Taken together, these data suggest the N-terminal extension may offer an alternative by imperfect "competitive" surface for luring He away from SJLEN, allowing some molecules to exist in the "open" form. The present data suggest that such a mechanism may operate in the holoreceptor, providing a mechanistic basis for integrin activation by inside-out signals. Variant Integrin Polypeptides Given the sequence similarity among the A domains of integrin α subunits, deletion of substitution of the He in a selected integrin α subunit that corresponds to He 332 of CDl lb should result in the creation of a variant integrin α subunit that is more active (i.e., in solution has a greater proportion of ligand binding form polypeptides) than the wild-type form of the subunit. Figure 5 is an alignment of the C-terminal α7 helix of the A domains of nine integrin α subunits (CDllb, CD lie, CD lid, CDlla, alpha 1, alpha 2, alpha 10, alpha 11, and alpha E). In this alignment, the invariant He corresponding to He 332 of CDllb in the other integrin α subunits is outlined (arrow). Replacing the invariant He with Ala or Gly or some other suitable amino acid should create a variant integrin polypeptide with increased activity. We have shown this to be the case in the CDl la A-domain (Figure 6). A variant CDlla A domain containing an He to Gly substitution displays binding to the activation dependent ligand ICAM-1 in an ELISA assay. No binding was observed in the wild-type protein without this substitution. Alternatively, the portion of the integrin α subunit (or the A domain) that includes the invariant He and all amino acid residues C terminal to the invariant He can be deleted. Table 2 below lists the position of the invariant He in each of the integrin subunits depicted in Figure 5. Table 2 The present invention features variant integrin α subunit polypeptides in which the invariant He (listed in Table 2) is substituted by Gly, Ala or some amino acid other than He (e.g., Val). The polypeptide can include part or all of the indicated A domain, e.g., 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, or 200 contiguous amino acids of the A domain that includes the position of the invariant He. The invention also includes variant integrin α subunits in which the He has been deleted. Also within the invention are polypeptides comprising the entire A domain of an integrin α except for the invariant He (e.g., amino acids 144-132 of CDllb, amino acids 145-332 of CDllc, amino acids 144-331 of CDlld, amino acids 150-330 of CDlla), but does not include the remainder of the integrin α subunit. The invention also features polypeptides comprising the A domain of an integrin α subunit up to but not including the invariant He and further lacking the 5 amino acids following the invariant He (e.g., amino acids 144-331, but not 332-336 of CDllb; amino acids 145-332, but not 333-337 of CDllc; amino acids 144-331, but not 332-336 of CDlld; amino acids 150-330, but not 331-335 of CDlla; amino acids 139-330, but not 331- 335 of human alpha 1; amino acids 169-360, but not 361-335 of human alpha 2; amino acids 57-248, but not 249-253 of human alpha 10; amino acids 159-348, but not 349-353 of human alpha 11; or amino acids 196-384, but not 385-389 of human alpha E). Also within the invention are variant integrin in which a change (substitution or deletion) of the He corresponding to He 332 of CDl lb α subunit is combined with one or more other activating mutations, e.g., an L 180 -F substitution or a mutation at E 147 , D 148 , K 247 orF 250 . Given the sequence similarity among the A-like domains of integrin β subunits, deletion or substitution of the He in a selected integrin β subunit that corresponds to He 332 of CDl lb should result in the creation of a variant integrin β subunit that is more active (i.e., in solution has a greater proportion of ligand binding form polypeptides) than the wild-type form of the subunit. Figure 7 is an alignment of the A-like domains of integrin β subunits. Replacing the conserved He with Ala or Gly or some other suitable amino acid should create a variant integrin polypeptide with increased activity. Alternatively, the portion of the integrin β subunit (or the A-like domain of the subunit) that includes the conserved He (or Leu) and all amino acid residues C terminal to the invariant He can be deleted. Table 3 below lists the position of the conserved He in each of the integrin β subunits depicted in Figure 7. Table 3 Nucleic Acid Molecules Encoding Variant Integrin Polypeptides The invention features isolated or purified nucleic acid molecules that encodes a variant integrin polypeptide, e.g., a full-length variant integrin subunit or a fragment thereof (e.g., in which the invariant He is deleted or substituted), e.g., a biologically active variant integrin polypeptide. In one embodiment, an isolated nucleic acid molecule of the invention includes the nucleotide sequence encoding amino acids 139-321 of the CDl lb α subunit (SEQ ID NO:l) or a portion thereof. The nucleic acid molecules can include non-coding sequences or sequences encoding all or a portion of a protein other than an integrin. In another embodiment, an isolated nucleic acid molecule of the invention includes a nucleic acid molecule which is a complement of the nucleotide sequence encoding amino acids 139-321 of the CDl lb α subunit. A nucleic acid molecule of the invention can include only a portion of the nucleic acid sequence of SEQ ID NO: 1. For example, such a nucleic acid molecule can include a fragment which encodes all or a portion (e.g., an immunogenic or biologically active portion) or an integrin A domain or A-like domain. Variant Integrin Polypeptides The present invention also includes variant integrin polypeptides. Such polypeptides can be produced using recombinant DNA methods, by chemical synthesis or using other techniques. The polypeptides can be used as immunogens or antigens generate antibodies that bind the active form of an integrin A-domain or A-like domain. The polypeptide can be post-translationally modified, e.g., glycosylated. A variant integrin polypeptide can be part of a fusion protein that includes all or a portion of a second polypeptide that is not a variant integrin polypeptide. This second polypeptide can be fused to the C-terminus or the N-terminus of the variant integrin polypeptide. All or part of a third peptide may also be present. Thus, a variant integrin polypeptide can be fused to, e.g., GST, an immunoglobulin constant region, a heterologous signal sequence. The variant integrin polypeptide fusion proteins of the invention can be incorporated into pharmaceutical compositions and administered to a subject in vivo. For example, the variant integrin polypeptides can be used to reduce skeletal muscle injury. Isolated CDl lb A domain has already been found to reduce damage in an animal model of this type of injury. Briefly, purified recombinant rat CDl lb A-domain was administered intravenously in a single dose (lmg/kg) to seven groups of Lewis rats (5 per group), 30 minutes before inducing mechanically skeletal muscle injury. Equal numbers of rats were treated with a function- blocking anti-CDllb/CD18 mAb (1 mg/kg). Quantitative histological examination of the wounded area in controlled rats (treated with PBS), showed edema, myofiber disruption, necrosis and erythrocytes extravasation. Influx of neutrophils was detected 30 minutes post wound, followed by a second wave 3 hours later. There was also significant tissue necrosis outside the immediate wounded area (5 mm zone) associated with the presence of activated neutrophils. A-domain or mAb-treated rats showed a comparable and significant decrease in the number of infiltrated PMN (75±10%, n=35) and a protection of the muscular fibers outside the immediate zone of necrosis (80±8%, n=35). These data show that the A-domain can be an effective tissue-preserving agent in this model of muscular injury. Variant A domain should be equally effective. The variant integrin polypeptide fusion proteins can be used to affect the bioavailability of a variant integrin polypeptide ligand. Variant integrin polypeptide fusion proteins may be useful therapeutically for the treatment of disorders caused by, for example, ischemia-reperfusion injury (Stroke 30:134- 9,1999), immune complexes (JExpMed 186:1853-63,1997), restenosis, and parasitic diseases (e.g. Ancylostoma spp; see J Cell Biol 127:2081-91, 1994). Moreover, the variant integrin polypeptide-fusion proteins of the invention can be used as immunogens to produce anti-variant integrin polypeptide antibodies in a subject, to purify variant integrin polypeptide ligands and in screening assays to identify molecules that inhibit the interaction of variant integrin polypeptide with an variant integrin polypeptide ligand. Expression vectors are commercially available that already encode a fusion moiety (e.g., a GST polypeptide). A variant integrin polypeptide-encoding nucleic acid can be cloned into such an expression vector such that the fusion moiety is linked in-frame to the variant integrin polypeptide protein. Variant integrin polypeptides can be produced using an expression vectors (e.g., a plasmid vector or a viral vector). The vector can be autonomously replicating or integrated in to the host genomes. The expression vector can include at least one regulatory sequence (e.g., promoter, enhancer, polyA site, or a cell- or tissue-specific transcription factor binding site) that is operatively linked to the nucleic acid sequence to be expressed. The expression vector can be designed for expression in prokaryotic or eukaryotic cells, e.g., plant cell, insect cells, yeast cells, mammalian cells, and E coli. Depending on the host cells used to express the variant integrin polypeptide, it may be desirable to encode the polypeptide using codons optimized for the host cell. In some cases it may be desirable to employ an expression vector capable of directing tissue-specific expression. The invention also features host cells or recombinant cells harboring a nucleic acid molecule encoding a variant integrin polypeptide. The nucleic acid molecule can be integrated into the host cells genome or present in an autonomously replicating vector. A host cell can be any prokaryotic or eukaryotic cell, e.g., E. coli, an insect cell, yeast or a mammalian cell. The nucleic acid molecules can be introduced into the host cells through transformation or transfection techniques. A host cell of the invention can be used to produce (i.e., express) a variant integrin polypeptide by culturing the host cell under conditions such that the polypeptide is produced and then isolating the polypeptide from the cells or the culture medium. Antibodies Recognizing Variant Integrin Polypeptides The invention also features antibodies directed against a variant integrin polypeptide. Such antibodies bind to the variant polypeptide with greater affinity than they bind to the corresponding wild-type integrin polypeptide. The antibodies can be generated using standard methods and can be screened by comparing the binding of the antibody to the variant integrin polypeptide to binding of the antibody to the corresponding wild-type polypeptide. The term "antibody" refers to an immunoglobulin molecule or immunologically active portion thereof, i.e., an antigen-binding portion. Examples of immunologically active portions of immunoglobulin molecules include F(ab) and F(ab')2 fragments which can be generated by treating the antibody with an enzyme such as pepsin. The antibody can be a polyclonal, monoclonal, recombinant, e.g., a chimeric or humanized, fully human, non- human, e.g., murine, or single chain antibody. In a preferred embodiment it has effector function. The antibody can be coupled to a toxin or imaging agent for use in diagnosis of occult inflammation (e.g. in abscess or active atherosclerotic plaques). Chimeric, humanized, but most preferably, completely human antibodies are desirable for applications which include repeated administration, e.g., therapeutic treatment (and some diagnostic applications) of human patients. The anti- variant integrin polypeptide antibody can be a single chain antibody which can be optionally dimerized or multimerized to generate multivalent antibodies. The antibody can be designed to have little or no ability to bind to an Fc receptor. The antibodies of the invention can be used to detect or purify integrin subunits that are in the active conformation. Thus, they can be used to evaluate the abundance and pattern of expression of an active form of an integrin as part of a clinical testing procedure. Detection can be facilitated by coupling (i.e., physically linking) the antibody to a detectable substance (i.e., antibody labeling). Examples of detectable substances include various enzymes, prosthetic groups, fluorescent materials, luminescent materials, bioluminescent materials, and radioactive materials (e.g., horseradish peroxidase, alkaline phosphatase, β- galactosidase, acetylcholinesterase, streptavidin/biotin and avidin/biotin, umbelliferone, fluorescein, fluorescein isothiocyanate, rhodamine, dichlorotriazinylamine fluorescent, dansyl chloride or phycoerythrin, luminol, luciferase, luciferin, aequorin, I, I, S and 3 H). Screening Assays to Identify Compounds that Interact with a Variant Integrin Polypeptide The invention features methods for evaluating a compound for the ability to bind to a variant integrin polypeptide or inhibit the ability of an integrin ligand (e.g., a naturally- occurring integrin ligand) to bind to a variant integrin polypeptide. The methods can include contacting the compound with the variant integrin polypeptide in the presence or absence of a ligand and measuring the ability of the compound or the ligand to bind to the variant polypeptide. The methods can be performed in vitro, e.g., in a cell free system, or in vivo, e.g., in a two-hybrid interaction trap assay. The method can be used to identify naturally- occurring molecules which interact with an integrin. It can also be used to find natural or synthetic inhibitors of the interaction between an integrin and an integrin ligand. The compounds tested can be, e.g., proteins, peptides, peptidomimetics, peptoids, and small molecules. The test compounds can be obtained from combinatorial libraries including: biological libraries; peptoid libraries; spatially addressable parallel solid phase or solution phase libraries; synthetic library methods requiring deconvolution; "one-bead one- compound" libraries. Libraries of compounds may be presented in solution (e.g., Houghten 1992 Biotechniques 13:412-421), or on beads (Lam 1991 Nature 354:82-84), chips (Fodor 1993 Nature 364:555-556), bacteria (Ladner U. S. Pat. 5,223,409), spores (Ladner U. S. Pat. 5,223,409), plasmids (Cull et al. 1992 Proc NatlAcadSci USA 89: 1865-1869) or on phage (Scott and Smith 1990 Science 249:386-390); Devlin 1990 Science 249:404-406; Cwirla et al. 1990 Proc. Natl. Acad. Sci. 87:6378-6382; Felici 1991 J. Mol. Biol. 222:301-310; Ladner, supra.). The screening assay can be a cell-based assay in which a cell which expresses an variant integrin polypeptide protein or biologically active portion thereof is contacted with a test compound, and the ability of the test compound to modulate variant integrin polypeptide activity is determined. Determining the ability of the test compound to modulate variant integrin polypeptide activity can be accomplished by monitoring, for example, the ability to bind an integrin ligand. The ability of the test compound to modulate variant integrin polypeptide binding to a compound, e.g., an integrin ligand or to bind to variant integrin polypeptide can also be evaluated. This can be accomplished, for example, by coupling the compound, e.g., the substrate, with a radioisotope or enzymatic label such that binding of the compound, e.g., the substrate, to variant integrin polypeptide can be determined by detecting the labeled compound, e.g., substrate, in a complex. Alternatively, a variant integrin polypeptide or the integrin ligand can be coupled with a radioisotope or enzymatic label to monitor the ability of a test compound to modulate variant integrin polypeptide binding to an integrin ligand. For 125 35 14 3 example, compounds can be labeled with I, S, C, or H, either directly or indirectly, and the radioisotope detected by direct counting of radioemmission or by scintillation counting. Alternatively, compounds can be enzymatically labeled with, for example, horseradish peroxidase, alkaline phosphatase, or luciferase, and the enzymatic label detected by determination of conversion of an appropriate substrate to product. In yet another embodiment, a cell-free assay is provided in which an variant integrin polypeptide or biologically active portion thereof is contacted with a test compound and the ability of the test compound to bind to the variant integrin polypeptide or biologically active portion thereof is evaluated. Preferred biologically active portions of the variant integrin polypeptide to be used in assays of the present invention include fragments that bind an integrin ligand. Soluble and/or membrane-bound forms of isolated proteins (e.g., variant integrin polypeptide proteins or biologically active portions thereof) can be used in the cell-free assays of the invention. When membrane-bound forms of the protein are used, it may be desirable to utilize a solubilizing agent. Examples of such solubilizing agents include non- ionic detergents such as n-octylglucoside, n-dodecylglucoside, n-dodecylmaltoside, octanoyl- N-methylglucamide, decanoyl-N-methylglucamide, Triton® X-100, Triton® X-l 14, Thesit®, Isotridecypoly(ethylene glycol ether) n , 3-[(3-cholamidopropyl)dimethylamminio]- 1 -propane sulfonate (CHAPS), 3-[(3-cholamidopropyl)dimethylamminio]-2-hydroxy-l- propane sulfonate (CHAPSO), or N-dodecyl=N,N-dimethyl-3-ammonio-l -propane sulfonate. Cell-free assays involve preparing a reaction mixture of the target gene protein and the test compound under conditions and for a time sufficient to allow the two components to interact and bind, thus forming a complex that can be removed and/or detected. The interaction between two molecules can also be detected, e.g., using fluorescence energy transfer (FET) (see, for example, Lakowicz et al, U.S. Patent No. 5,631, 169; Stavrianopoulos et al, U.S. Patent No. 4,868,103). A fluorophore label on the first, 'donor' molecule is selected such that its emitted fluorescent energy will be absorbed by a fluorescent label on a second, 'acceptor' molecule, which in turn is able to fluoresce due to the absorbed energy. Alternately, the 'donor' protein molecule may simply utilize the natural fluorescent energy of tryptophan residues. Labels are chosen that emit different wavelengths of light, such that the 'acceptor' molecule label may be differentiated from that of the 'donor'. Since the efficiency of energy transfer between the labels is related to the distance separating the molecules, the spatial relationship between the molecules can be assessed. In a situation in which binding occurs between the molecules, the fluorescent emission of the 'acceptor' molecule label in the assay should be maximal. An FET binding event can be conveniently measured through standard fluorometric detection means well known in the art (e.g., using a fluorimeter). In another embodiment, determining the ability of the variant integrin polypeptide protein to bind to a target molecule, e.g., an integrin ligand, can be accomplished using real- time Biomolecular Interaction Analysis (BIA) as described above (see, e.g., Sjolander and Urbaniczky (1991) Anal. Chem. 63:2338-2345 and Szabo et al (1995) Curr. Opin. Struct. Biol. 5:699-705). "Surface plasmon resonance" or "BIA" detects biospecific interactions in real time, without labeling any of the interactants (e.g., BIAcore). Changes in the mass at the binding surface (indicative of a binding event) result in alterations of the refractive index of light near the surface (the optical phenomenon of surface plasmon resonance (SPR)), resulting in a detectable signal that can be used as an indication of real-time reactions between biological molecules. In one embodiment, the polypeptide or the test compound is anchored onto a solid phase. The polypeptide/test compound complexes anchored on the solid phase can be detected at the end of the reaction. Preferably, the polypeptide can be anchored onto a solid surface, and the test compound, (which is not anchored), can be labeled, either directly or indirectly, with a detectable labels. It may be desirable to immobilize a variant integrin polypeptide, an anti-variant integrin polypeptide antibody or its target molecule to facilitate separation of complexed from uncomplexed forms of one or both of the proteins, as well as to accommodate automation of the assay. Binding of a test compound to a variant integrin polypeptide protein, or interaction of an variant integrin polypeptide protein with a target molecule in the presence and absence of a candidate compound, can be accomplished in any vessel suitable for containing the reactants. Examples of such vessels include microtiter plates, test tubes, and micro-centrifuge tubes. In one embodiment, a fusion protein can be provided which adds a domain that allows one or both of the proteins to be bound to a matrix. For example, glutathione-S-transferase/variant integrin polypeptide fusion proteins or glutathione-S- transferase/target fusion proteins can be adsorbed onto glutathione sepharose beads (Sigma Chemical, St. Louis, MO) or glutathione derivatized microtiter plates, which are then combined with the test compound or the test compound and either the non-adsorbed target protein or variant integrin polypeptide protein, and the mixture incubated under conditions conducive to complex formation (e.g., at physiological conditions for salt and pH). Following incubation, the beads or microtiter plate wells are washed to remove any unbound components, the matrix immobilized in the case of beads, complex determined either directly or indirectly, for example, as described above. Alternatively, the complexes can be dissociated from the matrix, and the level of variant integrin polypeptide binding or activity determined using standard techniques. Other techniques for immobilizing either a variant integrin polypeptide or a target molecule on matrices include using conjugation of biotin and streptavidin. Biotinylated variant integrin polypeptide or target molecules can be prepared from biotin-NHS (N- hydroxy-succinimide) using techniques known in the art (e.g., biotinylation kit, Pierce Chemicals, Rockford, JL), and immobilized in the wells of streptavidin-coated 96 well plates (Pierce Chemical). In order to conduct the assay, the non-immobilized component is added to the coated surface containing the anchored component. After the reaction is complete, unreacted components are removed (e.g., by washing) under conditions such that any complexes formed will remain immobilized on the solid surface. The detection of complexes anchored on the solid surface can be accomplished in a number of ways. Where the previously non- immobilized component is pre-labeled, the detection of label immobilized on the surface indicates that complexes were formed. Where the previously non-immobilized component is not pre-labeled, an indirect label can be used to detect complexes anchored on the surface; e.g., using a labeled antibody specific for the immobilized component (the antibody, in turn, can be directly labeled or indirectly labeled with, e.g., a labeled anti-Ig antibody). In one embodiment, this assay is performed utilizing antibodies reactive with variant integrin polypeptide or target molecules but which do not interfere with binding of the variant integrin polypeptide to its target molecule. Such antibodies can be derivatized to the wells of the plate, and unbound target or variant integrin polypeptide protein trapped in the wells by antibody conjugation. Methods for detecting such complexes, in addition to those described above for the GST-immobilized complexes, include immunodetection of complexes using antibodies reactive with the variant integrin polypeptide or target molecule, as well as enzyme-linked assays which rely on detecting an enzymatic activity associated with the variant integrin polypeptide or target molecule. Alternatively, cell free assays can be conducted in a liquid phase. In such an assay, the reaction products are separated from unreacted components, by any of a number of standard techniques, including chromatography, electrophoresis and immunoprecipitation. In a preferred embodiment, the assay includes contacting the variant integrin polypeptide or biologically active portion thereof with a known compound which binds variant integrin polypeptide to form an assay mixture, contacting the assay mixture with a test compound, and determining the ability of the test compound to interact with an variant integrin polypeptide protein, wherein determining the ability of the test compound to interact with an variant integrin polypeptide includes determining the ability of the test compound to preferentially bind to variant integrin polypeptide or biologically active portion thereof, or to modulate the activity of a target molecule, as compared to the known compound. To identify compounds that interfere with the interaction between a variant integrin polypeptide and an integrin ligand, a reaction mixture containing the variant integrin polypeptide and the ligand is incubated, under conditions and for a time sufficient, to allow the two products to form complex. In order to test an inhibitory agent, the reaction mixture is provided in the presence and absence of the test compound. The test compound can be initially included in the reaction mixture, or can be added at a time subsequent to the addition of the target gene and its cellular or extracellular binding partner. Control reaction mixtures are incubated without the test compound or with a placebo. The formation of any complexes between the variant integrin polypeptide and integrin ligand is then detected. The formation of a complex in the control reaction, but not in the reaction mixture containing the test compound, indicates that the compound interferes with the interaction. Additionally, complex formation within reaction mixtures containing the test compound and variant integrin polypeptide can also be compared to complex formation within reaction mixtures containing the test compound and the corresponding wild-type integrin polypeptide. These assays can be conducted in a heterogeneous or homogeneous format. Heterogeneous assays involve anchoring either the variant integrin polypeptide or the binding partner (i.e., the integrin ligand) onto a solid phase, and detecting complexes anchored on the solid phase at the end of the reaction. In homogeneous assays, the entire reaction is carried out in a liquid phase. In either approach, the order of addition of reactants can be varied to obtain different information about the compounds being tested. For example, test compounds that interfere with the interaction between the variant integrin polypeptides and the binding partner (e.g., an integrin ligand), e.g., , by competition, can be identified by conducting the reaction in the presence of the test substance. Alternatively, test compounds that disrupt preformed complexes, e.g., compounds with higher binding constants that displace one of the components from the complex, can be tested by adding the test compound to the reaction mixture after complexes have been formed. The various formats are briefly described below. In a heterogeneous assay system, either the variant integrin polypeptide or the integrin ligand, is anchored onto a solid surface (e.g., a microtiter plate), while the non-anchored species is labeled, either directly or indirectly. The anchored species can be immobilized by non-covalent or covalent attachments. Alternatively, an immobilized antibody specific for the species to be anchored can be used to anchor the species to the solid surface. In order to conduct the assay, the partner of the immobilized species is exposed to the coated surface with or without the test compound. After the reaction is complete, unreacted components are removed (e.g., by washing) and any complexes formed will remain immobilized on the solid surface. Where the non-immobilized species is pre-labeled, the detection of label immobilized on the surface indicates that complexes were formed. Where the non-immobilized species is not pre-labeled, an indirect label can be used to detect complexes anchored on the surface; e.g., using a labeled antibody specific for the initially non-immobilized species (the antibody, in turn, can be directly labeled or indirectly labeled with, e.g., a labeled anti-Ig antibody). Depending upon the order of addition of reaction components, test compounds that inhibit complex formation or that disrupt preformed complexes can be detected. Alternatively, the reaction can be conducted in a liquid phase in the presence or absence of the test compound, the reaction products separated from unreacted components, and complexes detected; e.g., using an immobilized antibody specific for one of the binding components to anchor any complexes formed in solution, and a labeled antibody specific for the other partner to detect anchored complexes. Again, depending upon the order of addition of reactants to the liquid phase, test compounds that inhibit complex or that disrupt preformed complexes can be identified. In an alternate embodiment of the invention, a homogeneous assay can be used. For example, a preformed complex of the variant integrin polypeptide and the integrin ligand is prepared in that either the variant integrin polypeptide or the integrin ligand is labeled, but the signal generated by the label is quenched due to complex formation (see, e.g., U.S. Patent No. 4,109,496 that utilizes this approach for immunoassays). The addition of a test substance that competes with and displaces one of the species from the preformed complex will result in the generation of a signal above background. In this way, test substances that disrupt variant integrin polypeptide-binding partner interaction can be identified. In yet another aspect, the variant integrin polypeptide proteins can be used as "bait proteins" in a two-hybrid assay or three-hybrid assay (see, e.g., U.S. Patent No. 5,283,317; Zervos et al. (1993) Cell 72:223-232; Madura et al. (1993) J. Biol. Chem. 268:12046-12054; Barrel et al (1993) Biotechniques 14:920-924; Iwabuchi et al. (1993) Oncogene 8: 1693- 1696; and Brent WO94/10300), to identify other proteins, which bind to or interact with variant integrin polypeptide. A two-hybrid assay can be carried out using a variant A domain as the bait protein. Briefly, the variant A domain is fused to the LexA DNA binding domain and used as bait. The prey is an aptamer library cloned into the active site loop of TrxA expression as a fusion protein with an N-terminal nuclear localization signal, a LexA activation domain, and an epitope tag (Colas et al 1996 Nature 380:548; and Gyuris et al. Cell 1993 75:791). Yeast cells are transformed with bait and prey genes. If an aptamer binds to variant A domain, the LexA activation domain is brought into proximity with the LexA DNA binding domain and expression of genes having an appropriately positioned LexA binding site increases. To test this system, yeast strain EGY48 was transformed with the bait plasmid and pSH18-34 (a URA3, 2 μm plasmid containing the GALl promoter fused to lacZ, in which the GALl enhancer- like Upstream Activating Sequence (UASG) has been replaced with binding sites for eight LexA dimers). The yeast strain contains two reporter genes, LexAop- LEU2 (replaces the yeast chromosomal LEU2 gene) and LexAop-lacZ, carried on a 2μm HIS3 + plasmid). Transformed cells were plated on medium containing X-gal The presence of the bait did not cause the expression of β-galactosidase. The bait by itself also did not activate the LexAop-Leu2 gene in EGY48, since the transformed cells did not grow on Leu deficient plates. Next, nuclear transport of the bait was assessed using the bait plasmid and a pJKlOl reporter in a repression type assay. In this assay DNA binding by transcriptionally inert LexA fusion proteins can be detected. The pJKlOl reporter is a URA3, 2 μm plasmid containing most of the UASG. A high affinity binding site for lexA is located between the Gall transcription start and the UASG. CDl IbA-LexA bait impaired the galactosidase activity of yeast harboring the pJKlOl plasmid when grown on galactose medium, indicating that this bait enters the nucleus. An aptamer library was introduced into the Leu and lacZ reporter-containing yeast cells. Synthesis of library proteins was induced by growing the yeast in galactose medium. In the absence of a suitable prey that binds the bait, the yeast cells does not grow on Leu" medium and have no β-galactosidase activity. A cell expressing a suitable prey will form colonies on Leu medium and have β -galactosidase activity. Selective galactose (but not glucose) inducible expression allows the Leu and LacZ phenotypes to be unambiguously ascribed to the library protein, diminishing the number of library plasmids that must be excluded by subsequent analysis. Plasmids from positive colonies (i.e., colonies able to grow on galactose, Ura", Tip" His" Leu" plates) were rescued (Hoffman et al. 1987 Gene 57:267). Before the respective clones were further characterized, the specificity of their interaction with the bait was tested. This was done by showing that they do not interact with unrelated or nonfunctional baits (e.g., a CDl la A-domain-lexA and 1 lbA-D242A-lexA respectively), with the DNA-binding domain portion of the bait or nonspecifically with the promoters or other elements of the transcription machinery. The library plasmids were rescued from the galactose-dependent Leu+ lacZ+ yeast and re-introduced into the original selection strain and into other strains containing different baits. Specific interactors confer the galactose- dependent Leu+ and lacZ+ phenotype to yeast containing the original bait, but not to yeast containing unrelated baits. An interaction mating assay can be used to test specificity. Briefly, a strain that contains the prey is mated with different yeast strains that express either the original bait protein or the control bait proteins. Reporters should only be active in diploids that contain the original bait (Finley et al. (1994) Proc. Nat'l Acad. Sci USA 91:12980). This invention further pertains to novel agents identified by the above-described screening assays. Accordingly, it is within the scope of this invention to further use an agent identified as described herein (e.g., an variant integrin polypeptide modulating agent) in an appropriate animal model to determine the efficacy, toxicity, side effects, or mechanism of action, of treatment with such an agent. Furthermore, novel agents identified by the above- described screening assays can be used for treatments as described herein. Pharmaceutical Compositions The variant integrin polypeptides, fragments thereof, as well as anti- variant integrin polypeptide antibodies (also referred to herein as "active compounds") of the invention can be incorporated into pharmaceutical compositions. Such compositions typically include the protein or antibody and a pharmaceutically acceptable carrier. As used herein the language "pharmaceutically acceptable carrier" includes solvents, dispersion media, coatings, antibacterial and anti-fungal agents, isotonic and absorption delaying agents, and the like, compatible with pharmaceutical administration. Supplementary active compounds can also be incorporated into the compositions. A pharmaceutical composition is formulated to be compatible with its intended route of administration. Examples of routes of administration include parenteral, intravenous, intradermal, subcutaneous, oral, inhalation, transdermal, transmucosal, and rectal administration. Solutions or suspensions used for parenteral, intradermal, or subcutaneous application can include the following components: a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as ethylenediaminetetraacetic acid; buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose. pH can be adjusted with acids or bases, such as hydrochloric acid or sodium hydroxide. The parenteral preparation can be enclosed in ampoules, disposable syringes or multiple dose vials made of glass or plastic. Pharmaceutical compositions suitable for injectable use include sterile aqueous solutions (where water soluble) or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersion. For intravenous administration, suitable carriers include physiological saline, bacteriostatic water, Cremophor EL™ (BASF, Parsippany, NJ) or phosphate buffered saline (PBS). In all cases, the composition must be sterile and should be fluid to the extent that easy syringability exists. It should be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyetheylene glycol, and the like), and suitable mixtures thereof. The proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants. Prevention of the action of microorganisms can be achieved by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, ascorbic acid, thimerosal, and the like. In many cases, it will be preferable to include isotonic agents, for example, sugars, polyalcohols such as manitol, sorbitol, sodium chloride in the composition. Prolonged absorption of the injectable compositions can be brought about by including in the composition an agent which delays absorption, for example, aluminum monostearate and gelatin. Sterile injectable solutions can be prepared by incorporating the active compound in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by filtered sterilization. Generally, dispersions are prepared by incorporating the active compound into a sterile vehicle which contains a basic dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum drying and freeze-drying which yields a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof. Oral compositions generally include an inert diluent or an edible carrier. For the purpose of oral therapeutic administration, the active compound can be incorporated with excipients and used in the form of tablets, troches, or capsules, e.g., gelatin capsules. Oral compositions can also be prepared using a fluid carrier for use as a mouthwash. Pharmaceutically compatible binding agents, and/or adjuvant materials can be included as part of the composition. The tablets, pills, capsules, troches and the like can contain any of the following ingredients, or compounds of a similar nature: a binder such as microcrystalline cellulose, gum tragacanth or gelatin; an excipient such as starch or lactose, a disintegrating agent such as alginic acid, Primogel, or corn starch; a lubricant such as magnesium stearate or Sterotes; a glidant such as colloidal silicon dioxide; a sweetening agent such as sucrose or saccharin; or a flavoring agent such as peppermint, methyl salicylate, or orange flavoring. For administration by inhalation, the compounds are delivered in the form of an aerosol spray from pressured container or dispenser which contains a suitable propellant, e.g., a gas such as carbon dioxide, or a nebulizer. Systemic administration can also be by transmucosal or transdermal means. For transmucosal or transdermal administration, penetrants appropriate to the barrier to be permeated are used in the formulation. Such penetrants are generally known in the art, and include, for example, for transmucosal administration, detergents, bile salts, and fusidic acid derivatives. Transmucosal administration can be accomplished through the use of nasal sprays or suppositories. For transdermal administration, the active compounds are formulated into ointments, salves, gels, or creams as generally known in the art. The compounds can also be prepared in the form of suppositories (e.g., with conventional suppository bases such as cocoa butter and other glycerides) or retention enemas for rectal delivery. In one embodiment, the active compounds are prepared with carriers that will protect the compound against rapid elimination from the body, such as a controlled release formulation, including implants and microencapsulated delivery systems. Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Methods for preparation of such formulations will be apparent to those skilled in the art. The materials can also be obtained commercially from Alza Corporation and Nova Pharmaceuticals, Inc. Liposomal suspensions (including liposomes targeted to infected cells with monoclonal antibodies to viral antigens) can also be used as pharmaceutically acceptable carriers. These can be prepared according to methods known to those skilled in the art, for example, as described in U.S. Patent No. 4,522,811. It is advantageous to formulate oral or parenteral compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used herein refers to physically discrete units suited as unitary dosages for the subject to be treated; each unit containing a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier. Toxicity and therapeutic efficacy of such compounds can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, e.g., for determining the LD50 (the dose lethal to 50% of the population) and the ED50 (the dose therapeutically effective in 50% of the population). The dose ratio between toxic and therapeutic effects is the therapeutic index and it can be expressed as the ratio LD50/ED50. Compounds which exhibit high therapeutic indeces are preferred. While compounds that exhibit toxic side effects may be used, care should be taken to design a delivery system that targets such compounds to the site of affected tissue in order to minimize potential damage to uninfected cells and, thereby, reduce side effects. The data obtained from the cell culture assays and animal studies can be used in formulating a range of dosage for use in humans. The dosage of such compounds lies preferably within a range of circulating concentrations that include the ED 50 with little or no toxicity. The dosage may vary within this range depending upon the dosage form employed and the route of administration utilized. For any compound used in the method of the invention, the therapeutically effective dose can be estimated initially from cell culture assays. A dose may be formulated in animal models to achieve a circulating plasma concentration range that includes the IC 50 (i.e., the concentration of the test compound which achieves a half-maximal inhibition of symptoms) as determined in cell culture. Such information can be used to more accurately determine useful doses in humans. Levels in plasma may be measured, for example, by high performance liquid chromatography. As defined herein, a therapeutically effective amount of protein or polypeptide (i.e., an effective dosage) ranges from about 0.001 to 30 mg/kg body weight, preferably about 0.01 to 25 mg/kg body weight, more preferably about 0.1 to 20 mg/kg body weight, and even more preferably about 1 to 10 mg/kg, 2 to 9 mg/kg, 3 to 8 mg/kg, 4 to 7 mg/kg, or 5 to 6 mg/kg body weight. The protein or polypeptide can be administered one time per week for between about 1 to 10 weeks, preferably between 2 to 8 weeks, more preferably between about 3 to 7 weeks, and even more preferably for about 4, 5, or 6 weeks. The skilled artisan will appreciate that certain factors may influence the dosage and timing required to effectively treat a subject, including but not limited to the severity of the disease or disorder, previous treatments, the general health and/or age of the subject, and other diseases present. Moreover, treatment of a subject with a therapeutically effective amount of a protein, polypeptide, or antibody can include a single treatment or, preferably, can include a series of treatments. For antibodies, the preferred dosage is 0.1 mg/kg of body weight (generally 10 mg/kg to 20 mg/kg). If the antibody is to act in the brain, a dosage of 50 mg/kg to 100 mg/kg is usually appropriate. Generally, partially human antibodies and fully human antibodies have a longer half-life within the human body than other antibodies. Accordingly, lower dosages and less frequent administration is often possible. Modifications such as lipidation can be used to stabilize antibodies and to enhance uptake and tissue penetration (e.g., into the brain). A method for lipidation of antibodies is described by Cruikshank et al ((1997) J. Acquired Immune Deficiency Syndromes and Human Retrovirology 14:193). The present invention encompasses agents which modulate expression or activity. An agent may, for example, be a small molecule. For example, such small molecules include, but are not limited to, peptides, peptidomimetics (e.g., peptoids), amino acids, amino acid analogs, polynucleotides, polynucleotide analogs, nucleotides, nucleotide analogs, organic or inorganic compounds (i.e,. including heteroorganic and organometallic compounds) having a molecular weight less than about 10,000 grams per mole, organic or inorganic compounds having a molecular weight less than about 5,000 grams per mole, organic or inorganic compounds having a molecular weight less than about 1,000 grams per mole, organic or inorganic compounds having a molecular weight less than about 500 grams per mole, and salts, esters, and other pharmaceutically acceptable forms of such compounds. Exemplary doses include milligram or microgram amounts of the small molecule per kilogram of subject or sample weight (e.g., about 1 microgram per kilogram to about 500 milligrams per kilogram, about 100 micrograms per kilogram to about 5 milligrams per kilogram, or about lmicrogram per kilogram to about 50 micrograms per kilogram. It is furthermore understood that appropriate doses of a small molecule depend upon the potency of the small molecule with respect to the expression or activity to be modulated. When one or more of these small molecules is to be administered to an animal (e.g., a human) in order to modulate expression or activity of a polypeptide or nucleic acid of the invention, a physician, veterinarian, or researcher may, for example, prescribe a relatively low dose at first, subsequently increasing the dose until an appropriate response is obtained. In addition, it is understood that the specific dose level for any particular animal subject will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, gender, and diet of the subject, the time of administration, the route of administration, the rate of excretion, any drug combination, and the degree of expression or activity to be modulated. An antibody (or fragment thereof) may be conjugated to a therapeutic moiety such as a cytotoxin, a therapeutic agent or a radioactive metal ion. A cytotoxin or cytotoxic agent includes any agent that is detrimental to cells. Examples include taxol, cytochalasin B, gramicidin D, ethidium bromide, emetine, mitomycin, etoposide, tenoposide, vincristine, vinblastine, colchicin, doxorubicin, daunorubicin, dihydroxy anthracin dione, mitoxantrone, mithramycin, actinomycin D, 1-dehydrotestosterone, glucocorticoids, procaine, tetracaine, lidocaine, propranolol, and puromycin and analogs or homologs thereof. Therapeutic agents include, but are not limited to, antimetabolites (e.g., methotrexate, 6-mercaptopurine, 6- thioguanine, cytarabine, 5-fluorouracil decarbazine), alkylating agents (e.g., mechlorethamine, thioepa chlorambucil, melphalan, carmustine (BSNU) and lomustine (CCNU), cyclothosphamide, busulfan, dibromomannitol, streptozotocin, mitomycin C, and cis-dichlorodiamine platinum (H) (DDP) cisplatin), anthracyclines (e.g., daunorubicin (formerly daunomycin) and doxorubicin), antibiotics (e.g., dactinomycin (formerly actinomycin), bleomycin, mithramycin, and anthramycin (AMC)), and anti-mitotic agents (e.g., vincristine and vinblastine). The conjugates of the invention can be used for modifying a given biological response, the drug moiety is not to be construed as limited to classical chemical therapeutic agents. For example, the drug moiety may be a protein or polypeptide possessing a desired biological activity. Such proteins may include, for example, a toxin such as abrin, ricin A, pseudomonas exotoxin, or diphtheria toxin or a protein such as a cytokine or interleukin. Alternatively, an antibody can be conjugated to a second antibody to form an antibody heteroconjugate as described by Segal in U.S. Patent No. 4,676,980. The nucleic acid molecules of the invention can be inserted into vectors and used as gene therapy vectors. Gene therapy vectors can be delivered to a subject by, for example, intravenous injection, local administration (see U.S. Patent 5,328,470) or by stereotactic injection (see e.g., Chen et al (1994) Proc. Natl. Acad. Sci. USA 91:3054-3057). The pharmaceutical preparation of the gene therapy vector can include the gene therapy vector in an acceptable diluent, or can comprise a slow release matrix in which the gene delivery vehicle is imbedded. Alternatively, where the complete gene delivery vector can be produced intact from recombinant cells, e.g., retroviral vectors, the pharmaceutical preparation can include one or more cells which produce the gene delivery system. The pharmaceutical compositions can be included in a container, pack, or dispenser together with instructions for administration. Animal Models of Processes Involving Integrins A model of vascular injury that can be useful in testing potential therapeutic compositions is described by Simon et al. (J. Clin. Invest. 2000 105:293). Tang et al. (1997 J ExpMed 186:1853) describe CDl lb knockout mice that can be useful in various screening assays. An animal model of burn injury may also be useful (Plast Reconstr Surg 1995 96:1177). Methods of Treatment The present invention provides for both prophylactic and therapeutic methods of treating a subject at risk of (or susceptible to) a disorder or having a disorder associated with aberrant or unwanted integrin expression or activity. "Treatment" or "treating a patient", as used herein, is defined as the application or administration of a therapeutic agent to a patient, or application or administration of a therapeutic agent to an isolated tissue or cell line from a patient, who has a disease, a symptom of disease or a predisposition toward a disease, with the purpose to cure, heal, alleviate, relieve, alter, remedy, ameliorate, palliate, improve or affect the disease, the symptoms of disease or the predisposition toward disease. A therapeutic agent includes, but is not limited to, small molecules, peptides, antibodies, ribozymes and antisense oligonucleotides. Toxicity and therapeutic efficacy of such compounds can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, e.g., for determining the LD50 (the dose lethal to 50% of the population) and the ED50 (the dose therapeutically effective in 50% of the population). The dose ratio between toxic and therapeutic effects is the therapeutic index and it can be expressed as the ratio LD 5 0/ED 50 . Compounds that exhibit large therapeutic indices are preferred. While compounds that exhibit toxic side effects can be used, care should be taken to design a delivery system that targets such compounds to the site of affected tissue in order to minimize potential damage to uninfected cells and, thereby, reduce side effects.

Claims: WHAT IS CLAIMED IS: 1. An isolated nucleic acid molecule encoding a variant CDl lb α subunit having the He at position 332 replaced with an amino acid selected from Gly and Ala. 2. A variant CDl lb α subunit polypeptide having the He at position 332 replaced with an amino acid selected from Gly and Ala. 3. A isolated nucleic acid molecule encoding a polypeptide consisting of amino acids 144 to 331 of CDl lb α subunit. 4. An isolated nucleic acid molecule encoding a polypeptide comprising amino acids 144 to 332 of CDl lb α subunit wherein the He at amino acid 332 has been replaced by an amino acid selected from Gly and Ala. 5. A polypeptide comprising amino acids 144 to 332 of CDl lb α subunit wherein the He at position 332 has been replaced by an amino acid selected from Gly and Ala. 6. A polypeptide comprising amino acids 144 to 331 of CDl lb α subunit, the polypeptide not comprising amino acids 332 to 1152 of CDl lb α subunit. 7. An isolated nucleic acid molecule encoding a variant CDl la α subunit having the He at position 331 replaced with an amino acid selected from Gly and Ala. 8. A variant CDl la α subunit having the He at position 331 replaced with an amino acid selected from Gly and Ala. 9. An isolated nucleic acid molecule encoding a polypeptide consisting of amino acids 150 to 330 of CDl la α subunit. 10. An isolated nucleic acid molecule encoding a polypeptide comprising amino acids 150 to 331 of CDl la α subunit wherein the He at amino acid 331 has been replaced by an amino acid selected from Gly and Ala. 11. A polypeptide comprising amino acids 150 to 331 of CD 11 a α subunit wherein the He at position 331 has been replaced by an amino acid selected from Gly and Ala. 12. A polypeptide comprising amino acids 150 to 330 of CDl la α subunit, the polypeptide not comprising amino acids 331 to 1223 of CDl la. 13. A method for determining whether a test compound is a candidate compound for binding to CDl lb, comprising: (a) contacting a test compound with a polypeptide comprising amino acids 144 to 332 of CDl lb α subunit wherein the He at amino acid 332 has been replaced by an amino acid selected from Gly and Ala, and (b) determining whether the test compound binds to the polypeptide, wherein a compound which binds to the polypeptide is a candidate compound for binding to CDl lb. 14. A method for generating an antibody that selectively binds to a polypeptide comprising the open form of CDl lb, the method comprising inoculating a non-human mammal with a polypeptide comprising amino acids 144 to 332 of CDl lb α subunit wherein the He at amino acid 332 has been replaced by an amino acid selected from Gly and Ala; and isolating from the mammal an antibody that selectively bids to a polypeptide comprising the open form of CD lib. 15. An antibody that selectively binds to a polypeptide consisting of acids 144 to 332 of CDl lb α subunit wherein the He at amino acid 332 has been replaced by an amino acid selected from Gly and Ala. 16. The antibody of claim 15 wherein the antibody is a monoclonal antibody. 17. A method for determining whether a test compound is a candidate compound for treating an inflammatory disorder, comprising: (a) contacting a test compound with a polypeptide comprising amino acids 144 to 332 of CDl lb α subunit wherein the He at amino acid 332 has been replaced by an amino acid selected from Gly and Ala, and (b) determining whether the test compound binds to the polypeptide, wherein a compound which binds to the polypeptide is a candidate compound for treating an inflammatory disorder. 18. An isolated nucleic acid molecule encoding a polypeptide comprising amino acids 144 to 320 of CDl lb α subunit wherein the Phe at amino acid 313 and the Ala at amino acid 320 have been replaced by Cys. 19. A polypeptide comprising amino acids 144 to 320 of CDl lb α subunit wherein the Phe at amino acid 313 and the Ala at amino acid 320 have been replaced by Cys. 20. An isolated nucleic acid molecule encoding a polypeptide comprising amino acids 144 to 320 of CDl lb α subunit wherein the Val at amino acid 315 and the Ala at amino acid 320 have been replaced by Cys. 21. A polypeptide comprising amino acids 144 to 320 of CDl lb α subunit wherein the Val at amino acid 315 and the Ala at amino acid 320 have been replaced by Cys. 22. A method for determining whether a test compound is a candidate compound for binding to CDl lb, comprising: (a) contacting a test compound with a polypeptide comprising amino acids 144 to 320 of CDl lb α subunit wherein the Phe at amino acid 313 and the Ala at amino acid 320 have been replaced by Cys, and (b) determining whether the test compound binds to the polypeptide, wherein a compound which binds to the polypeptide is a candidate compound for binding to CDl lb. 23. A method for generating an antibody that selectively binds to a polypeptide comprising the open form of CDl lb, the method comprising inoculating a non-human mammal with a polypeptide comprising amino acids 144 to 320 of CDl lb α subunit wherein the Phe at amino acid 313 and the Ala at amino acid 320 have been replaced by Cys; and isolating from the mammal an antibody that selectively bids to a polypeptide comprising the open form of CDl lb. 24. An antibody that selectively binds to a polypeptide consisting of amino acids 144 to 320 of CDl lb α subunit wherein the Phe at amino acid 313 and the Ala at amino acid 320 have been replaced by Cys. 25. The antibody of claim 24 wherein the antibody is a monoclonal antibody. 26. A method for determining whether a test compound is a candidate compound for treating an inflammatory disorder, comprising: (a) contacting a test compound with a polypeptide comprising amino acids 144 to 320 of CDl lb α subunit wherein the Phe at amino acid 313 and the Ala at amino acid 320 have been replaced by Cys, and (b) determining whether the test compound binds to the polypeptide, wherein a compound which binds to the polypeptide is a candidate compound for treating an inflammatory disorder. 27. A method for determining whether a test compound is a candidate compound for binding to CDl lb, comprising: (a) contacting a test compound with a polypeptide comprising amino acids 144 to 320 of CDllb α subunit wherein the Val at amino acid 315 and the Ala at amino acid 320 have been replaced by Cys, and (b) determining whether the test compound binds to the polypeptide, wherein a compound which binds to the polypeptide is a candidate compound for binding to CDl lb. 28. A method for generating an antibody that selectively binds to a polypeptide comprising the open form of CD lib, the method comprising inoculating a non-human mammal with a polypeptide comprising amino acids 144 to 320 of CDl lb α subunit wherein the Val at amino acid 315 and the Ala at amino acid 320 have been replaced by Cys; and isolating from the mammal an antibody that selectively bids to a polypeptide comprising the open form of CDl lb. 29. An antibody that selectively binds to a polypeptide consisting of amino acids 144 to 320 of CDl lb α subunit wherein the Val at amino acid 315 and the Ala at amino acid 320 have been replaced by Cys. 30. The antibody of claim 29 wherein the antibody is a monoclonal antibody. 31. A method for determining whether a test compound is a candidate compound for treating an inflammatory disorder, comprising: (a) contacting a test compound with a polypeptide comprising amino acids 144 to 320 of CDl lb α subunit wherein the Val at amino acid 315 and the Ala at amino acid 320 have been replaced by Cys, and (b) determining whether the test compound binds to the polypeptide, wherein a compound which binds to the polypeptide is a candidate compound for treating an inflammatory disorder. 32. An isolated nucleic acid molecule encoding a variant CDl lb α subunit having the He at position 332 replaced with an amino acid other than He. 33. A variant CDl lb α subunit polypeptide having the He at position 332 replaced with an amino acid other than He. 34. An isolated nucleic acid molecule encoding a polypeptide comprising amino acids 144 to 332 of CDl lb α subunit wherein the He at amino acid 332 has been replaced by an amino acid other than He. 35. A polypeptide comprising amino acids 144 to 332 of CDl lb α subunit wherein the He at position 332 has been replaced by an amino acid selected from Gly and Ala. 36. An isolated nucleic acid molecule encoding a variant CDl lc α subunit having the He at position 333 replaced with an amino acid other than He. 37. A variant CDl lc α subunit polypeptide having the He at position 333 replaced with an amino acid other than He. 38. A isolated nucleic acid molecule encoding a polypeptide consisting of amino acids 144 to 332 of CD 11 c α subunit. 39. An isolated nucleic acid molecule encoding a polypeptide comprising amino acids 144 to 333 of CDl lc α subunit wherein the He at amino acid 332 has been replaced by an amino acid other than He. 40. An isolated nucleic acid molecule encoding a variant CDl Id a subunit having the He at position 332 replaced with an amino acid other than He. 41. A variant CDl Id α subunit polypeptide having the He at position 332 replaced with an amino acid other than He. 42. A isolated nucleic acid molecule encoding a polypeptide consisting of amino acids 144 to 331 of CDl Id α subunit. 43. An isolated nucleic acid molecule encoding a polypeptide comprising amino acids 144 to 332 of CDl Id α subunit wherein the He at amino acid 332 has been replaced by an amino acid other than He. 44. An isolated nucleic acid molecule encoding a variant CD49a α subunit having the He at position 331 replaced with an amino acid other than He. 45. A variant CD49a α subunit polypeptide having the He at position 331 replaced with an amino acid other than He. 46. A isolated nucleic acid molecule encoding a polypeptide consisting of amino acids 144 to 330 of CD49a α subunit. 47. An isolated nucleic acid molecule encoding a polypeptide comprising amino acids 144 to 332 of CD49a α subunit wherein the He at amino acid 331 has been replaced by an amino acid other than He. 48. An isolated nucleic acid molecule encoding a variant CD49b α subunit having the He at position 361 replaced with an amino acid other than He. 49. A variant CD49b α subunit polypeptide having the He at position 361 replaced with an amino acid other than He. 50. A isolated nucleic acid molecule encoding a polypeptide consisting of amino acids 144 to 360 of CD49b α subunit. 51. An isolated nucleic acid molecule encoding a polypeptide comprising amino acids 144 to 361 of CD49b α subunit wherein the He at amino acid 361 has been replaced by an amino acid other than He. 52. An isolated nucleic acid molecule encoding a variant Alpha 10 α subunit having the He at position 249 replaced with an amino acid other than He. 53. A variant Alpha 10 α subunit polypeptide having the He at position 249 replaced with an amino acid other than He. 54. A isolated nucleic acid molecule encoding a polypeptide consisting of amino acids 57 to 248 of Alpha 10 α subunit. 55. An isolated nucleic acid molecule encoding a polypeptide comprising amino acids 57 to 249 of Alpha 10 α subunit wherein the He at amino acid 249 has been replaced by an amino acid other than He. 56. An isolated nucleic acid molecule encoding a variant Alpha 11 α subunit having the He at position 349 replaced with an amino acid other than He. 57. A variant Alpha 11 α subunit polypeptide having the He at position 349 replaced with an amino acid other than He. 58. A isolated nucleic acid molecule encoding a polypeptide consisting of amino acids 159 to 348 of Alpha 11 α subunit. 59. An isolated nucleic acid molecule encoding a polypeptide comprising amino acids 159 to 349 of Alpha 11 α subunit wherein the He at amino acid 349 has been replaced by an amino acid other than He. 60. An isolated nucleic acid molecule encoding a variant Alpha E α subunit having the He at position 385 replaced with an amino acid other than He. 61. A variant Alpha 11 α subunit polypeptide having the He at position 385 replaced with an amino acid other than He. 62. A isolated nucleic acid molecule encoding a polypeptide consisting of amino acids 196 to 384 of Alpha 11 α subunit. 63. An isolated nucleic acid molecule encoding a polypeptide comprising amino acids 196 to 387 of Alpha 11 α subunit wherein the He at amino acid 385 has been replaced by an amino acid other than He. 64. The isolated nucleic acid molecule of any of claims 32, 34, 36, 38, 39, 40, 42, 43, 44, 46, 47, 48, 50, 51, 52, 54, 56, 58, 59, 60, 62, and 63 wherein the amino acids other than He is selected from the group consisting of Gly and Ala. 65. The polypeptide of any of claims 33, 35, 37, 41, 45, 49, 53, 55, 57, and 61 wherein the amino acid other than He is selected from the group consisting of Gly and Ala. 66. A method for treating an inflammatory disorder, the method comprising administering the antibody of any of claims 15, 24, and 29. 67. A method for treating ischemia-reperfiision injury, the method comprising administering the antibody of any of claims 15, 24, 29. 68. A method for treating restinosis, the method comprising administering the antibody of any of claims 15, 24, and 29.

Inventors: ARNAOUT AMIN M, LI RUI, XIONG JIAN-PING, ARNAOUT, AMIN, M., LI, RUI, XIONG, JIAN-PING
Applicants: ARNAOUT AMIN M, GEN HOSPITAL CORP, LI RUI, XIONG JIAN PING, ARNAOUT, AMIN, M., LI, RUI, THE GENERAL HOSPITAL CORPORATION, XIONG, JIAN-PING
Application year: 2001
Main classification: A61K38
IPC codes: A61K38, C07K14, C07K16