EXPRESSION OF HERSTATIN, AN ALTERNATIVE HER-2/NEU PRODUCT, IN CELLS THAT EXPRESS EITHER P185HER-2 OR THE EGF RECEPTOR INHIBITS RECEPTOR ACTIVITY AND CELL GROWTH

Abstract: An alternative HER-2/neu product, herstatin, consists of subdomains I and II from the ectodomain of p185HER-2 and a unique 79 amino acid C-terminus encoded by intron 8. Recombinant herstatin added to cells was found to bind to and inhibit p185HER-2. The effects of ectopic expression of herstatin in combination with either p185HER-2 or with its homolog, the EGF receptor, in several cell lines was studied. Cotransfection of herstatin with HER-2 inhibited p185HER-2 levels and caused an approximate 8-fold reduction in p185 tyrosine phosphorylation. Inhibition of p185HER-2 tyrosine phosphorylation corresponded to a dramatic decline in colony formation by cells that coexpressed p185HER-2 and herstatin. Herstatin also interferred with EGF activation of the EGF receptor in cotransfected cells as demonstrated by impaired receptor tyrosine phosphorylation, reduced receptor down-regulation, and growth suppression. For both p185HER-2 and the EGF receptor, the extent of inhibition was affected by the expression levels of herstatin relative to the receptor. Herstatin is an autoinhibitor of p185HER-2 and expands its inhibitory activity to another member of the group I family of receptor tyrosine kinases, the EGF receptor. Herstatin blocked the activated Akt-mediated EGF survival signal, as well as transforming growth factor alpha (TGF alpha )-mediated EGF receptor activation, survival signal and proliferation signal. Purified recombinant herstatin specifically inhibited human carcinoma cells that over-express HER-2, and was effectively absorbed into the blood of intraperitoneally injected mice, where it was not proteolytically degraded and was present for between one and three hours.

Description: EXPRESSION OF HERSTATIN, AN ALTERNATIVE HER-2/NEU PRODUCT, IN CELLS THAT EXPRESS EITHER P185HER-2 OR THE EGF RECEPTOR INHIBITS RECEPTOR ACTIVITY AND CELL GROWTH CROSS-REFERENCE TO RELATED APPLICATIONS This application is a continuation-in-part of United States Patent Application Serial No. 09/506,079, entitled HER-2 BINDING ANTAGONIST, filed February 16, 2000, which is a continuation-in-part of United States Patent Application Serial No. 09/234,208, entitled HER-2 BINDING ANTAGONISTS, filed 20 January 1999. BACKGROUND OF THE INVENTION A HER-2 binding antagonist is described and provided. Specifically, intron retention has generated a novel HER-2 antagonist polypeptide that binds to the HER-2 receptor. This work was supported by a grant from the Department of Defense (DOD) Breast Cancer Research Program. The United States Government has certain rights in this invention. The HER-2/neu (erbB-2) oncogene encodes a receptor-like tyrosine kinase (RTK) that has been extensively investigated because of its role in several human carcinomas (Hynes and Stem, Biochim. eiBiophys. Acta 1198:165-184, 1994; and Dougall et al., Oncogene 9:2109-2123, 1994) and in mammalian development (Lee et al., Nature 378:394-398, 1995). The sequence of the HER-2 protein was determined from a cDNA that was cloned by homology to the epidermal growth factor receptor (EGFR) mRNA from placenta (Coussens et al., Science 230: 1132-1139, 1985) and from a gastric carcinoma cell line (Yamamoto et al., Nature 319:230-234, 1986). The HER-2 mRNA was shown to be about 4.5 kb (Coussens et al., Science 230:1132-1139, 1985; and Yamamoto et al., Nature 319:230-234, 1986) and encodes a transmembrane glycoprotein of 185 kDa in normal and malignant human tissues (pl85HER-2) (Hynes and Stem, Biochim. et Biophys. Acta 1198:165-184, 1994; and Dougall et al., Oncogene 9:2109- 2123, 1994). The function of the HER-2 gene has been examined mainly by expressing the cDNA coπesponding to the 4.5 kb transcript in transfected cells and from the structure and biochemical properties of the 185 kDa protein product. P185HER-2 consists of a large extracellular domain, a transmembrane segment, and an intracellular domain with tyrosine kinase activity (Hynes and Stern, Biochim. et Biophys. Acta 1198:165-184, 1994; and Dougall et al., Oncogene 9:2109-2123, 1994). Overexpression of pl85HER-2 causes phenotypic transformation of cultured cells (DiFiore et al., Science 237:178-182, 1987; and Hudziak et al., Proc. Natl. Acad. Sci. USA 84:7159-7163, 1987) and has been associated with aggressive clinical progression of breast and ovarian cancer (Slamon et al., Science 235:177-182, 1987; and Slamon et al., Science 244:707-712, 1989). pl85HER-2 is highly homologous to the EGFR. However, a ligand that directly binds with high affinity to pl 85HER-2 has not yet been identified. Moreover, the signaling activity of HER-2 may be mediated through heterodimerization with other ligand-binding members of the EGFR family (Caπaway and Cantley, Cell 78:5-8, 1994; Earp et al., Breast Cancer Res. Treat. 35:115-132, 1995; and Qian et al., Oncogene 10:211-219, 1995). Divergent proteins, containing regions of the extracellular domains of HER family RTKs, are generated through proteolytic processing of full length receptors (Lin and Clinton, Oncogene 6:639-643, 1991; Zabrecky et al., J. Biol. Chem. 266:1716-1720, 1991; Pupa et al., Oncogene 8:2917-2923, 1993; Vecchi et al., J. Biol. Chem. 211:18989- 18995, 1996; and Vecchi and Carpenter, J. Cell Biol. 139:995-1003, 1997) and through alternative RNA processing (Petch et al., Mol. Cell Biol. 10:2973-2982, 1990; Scott et al., Mol. Cell. Biol. 13:2247-2257, 1993; and Lee and Maihle, Oncogene 16:3243-3252, 1998). The extracellular domain of pl85HER-2 is proteolytically shed from breast carcinoma cells in culture (Petch et al., Mol. Cell. Biol. 10:2973-2982, 1990; Scott et al., Mol. Cell. Biol. 13:2247-2257, 1993; and Lee and Maihle, Oncogene 16:3243-3252, 1998), and is found in the serum of some cancer patients (Leitzel et al., J. Clin. Oncol. 10:1436-1443, 1992) where it is may be a serum marker of metastatic breast cancer (Leitzel et al., J Clin. Oncol. 10:1436-1443, 1992) and may allow escape of HER-2-rich tumors from immunological control (Baselga et al., J Clin. Oncol. 14:737-744, 1966; and Brodowicz et al., Int. J. Cancer 73:875-879, 1997). A truncated extracellular domain of HER-2 is also the product of a 2.3 kb alternative transcript generated by use of a polyadenylation signal within an intron (Scott et al., Mol Cell. Biol. 13:2247-2257, 1993). The alternative transcript was first identified in the gastric carcinoma cell line, MKN7 (Yamamoto et al., Nature 319:230- 234, 1986; and Scott et al., Mol. Cell. Biol. 13:2247-2257, 1993) and the truncated receptor was located within the perinuclear cytoplasm rather than secreted from these tumor cells (Scott et al., Mol. Cell. Biol. 13:2247-2257, 1993). However, no particular therapeutic, diagnostic or research utility has been ascribed to this truncated extracellular domain polypeptide. A truncated extracellular domain of the EGFR, generated by alternative splicing (Petch et al., Mol. Cell. Biol. 10:2973-2982, 1990) is secreted, exhibits ligand-binding, and dimerization properties (Basu et al., Mol. Cell. Biol. 9:671- 677, 1989), and may have a dominant negative effect on receptor function (Basu et al., Mol. Cell. Biol. 9:611-611, 1989; and Flickinger et al., Mol. Cell. Biol. 12:883-893, 1992). Group I receptor tyrosine kinases including the EGF-receptor (HER-1, erbB-1), HER-2 (erbB-2), HER-3 (erbB-3), and HER-4 (erbB-4) are widely expressed in epithelial, mesenchymal, and neuronal tissues and play fundamental roles in proliferation and differentiation. With the exception of pl85HER-2, receptor tyrosine kinases are activated by binding to a variety of EGF-related growth factors. Ligand binding is coupled to receptor dimerization, tyrosine autophosphorylation, and signal activation. Independent of specific growth factor binding, pl85HER-2 dimerizes with itself, or is recruited as the prefeπed heterodimer partner where it transactivates other receptor family members. Enhanced amounts of group I receptors at the cell membrane occurs frequently in human carcinomas. This elevation in number of receptors is likely to favor the formation of receptor oligomers resulting in amplified signaling. The EGF-receptor and pl85 HER-2 have been most frequently and clearly associated with human malignancies. HER-2 is overexpressed in breast, ovarian, gastric, and endometrial carcinomas. Elevated levels of pl85HER-2 in 25-30% of breast and ovarian cancers is predictive of significantly lower survival rates and shorter relapse times. Amplification and alteration of the EGF-receptor gene is often observed in squamous cell carcinoma of the lung (Pavelic et al., 1993) and in glial tumors (Libermann et al., 1985), particularly in glioblastoma, the most malignant glial tumor. There have been extensive efforts directed toward defining the structure and function of the group I receptor extracellular domains in the interests of understanding the mechanism of receptor activation and in blocking receptor action at the cell surface. Receptor mutants consisting of the extracellular domain and a membrane anchor, in the absence of the cytoplasmic domain, are capable of dimerizing (Lemmon et al., 1997; Tzahar et al., 1997;Tanner and Kyte 1999) and forming kinase inactive complexes with cell surface receptors (Greene). The ectodomains of group I receptors have been divided into subdomains I (beginning at the N-terminus) through TV, ending at the juxtamembrane position. Domains II and IV contain multiple cysteine residues that are conserved amongst the four group I receptors. Subdomains I and II appear to be a repeating unit of III and IV that may have arisen by a gene duplication event (Ullrich et al., 1984). Deletion of subdomains I and II from the EGF receptor results in constitutive dimerization and oncogenic transfoπnation in a ligand-independent fashion (Hayely et al., 1989; Carter and Kung 1994; Qian et al., 1994; Moscatello et al, 1996), and allows ligand independent heterodimer formation with the membrane anchored p 185neu ectodomain mutant (Greene). While subdomain III contains the high-affinity ligand binding site as shown for EGF binding to the EGF receptor (Wu et al., 1990 Woltjer et al., 1992 Lax et al., 1989; 1991), subdomain I has been suggested to serve as a low- affinity site that is promiscuous in its ligand recognition (Lax et al., 1989; 1991 Tzahar et al., 1997). According to this model EGF-like ligands are bivalent with a high affinity site that binds to the direct receptor in subdomain III and a second, low affinity site with broad specificity for subdomain I that prefers interaction with pl85HER-2, thereby explaining the status of pl85HER-2 as the prefeπed dimer partner. Taken together these results suggest that subdomains I and II may exert a negative constraint on dimerization in the absence of ligand and could be important for recruitment of receptors into heterodimers. Monoclonal antibodies against the ectodomains of pl85HER-2 and the EGF-receptor have been shown to be effective in limiting growth of tumors. These antibodies bind to their receptor targets with high affinity and specificity and their toxicity is low. The mechanisms underlying the anti-tumorigenic effects of antibodies are unclear. The rhuMAb4D5 monoclonal antibody (Herceptin™) may act by down- regulation of pl85HER-2 at the cell surface (Hurwitz et al., 1995), which causes a reversible cytostatic effect on HER-2 mediated cell growth. Systemic administration of Herceptin™ has been shown to have therapeutic efficacy, because it increases the time to recurrence in a subset of patients with metastatic breast cancer. High-affinity humanized, monoclonal antibodies against the EGF receptor have also been used as antitumor agents. While the molecular mechanisms underlying the activity of EGF receptor antibodies remains elusive, those tested compete with growth factor binding. Antibody strategies that target pl85HER-2 and the EGF-receptor, as well as heterodimers between these two receptors, have also been attempted. Preliminary evidence suggests that targeting both receptors may significantly augment antiproliferative effects. Mutant receptors consisting of ectodomains have proved to be effective in inhibition of tumorigenesis. The membrane-anchored ectodomain of p 185neu, ectopically expressed in cells, functions as a dominant-negative inhibitor, based on its ability to dimerize with the ectodomains of group I receptors forming a kinase-inactive complex. P185neu ectodomain mutants are capable of specific inhibition of pl85HER-2 homodimer signaling as well as trans-inhibition of EGF receptor signaling. The pl85ectodomain is capable of suppressing the activation of all group I receptors, because 185HER-2 is the prefeπed heterodimer partner of group I RTKs. However, membrane anchoring of ectodomain mutants is required to exert the dominant-negative effect, because interactions between soluble ectodomains and cell surface receptors are too weak to achieve complex formation. SUMMARY OF THE INVENTION The present invention is directed to a naturally occuring inhibitor of the HER-2 receptor tyrosine kinase called herstatin which consists of the first 340 aa-residues identical to N-terminal subdomains I and II of pl85HER-2, followed by a novel C-terminus of 79 aa-residues and a stop codon specified by the inserted sequence. In contrast to soluble ectodomains, herstatin binds with high affinity (~ 14 nM Kd) to cell surface pl85HER-2. Although herstatin is secreted and complexes with l85HER-2 at the cell surface, it differs from other EGR-like ligands in its ability to inhibits the activity of pl85HER-2. In the current study we find that coexpression of herstatin with pl85HER-2 causes a striking reduction in cell growth that coπesponds with suppression of pl85 autophosphorylation. Moreover the inhibitory activity of herstatin is extended to EGF activation of the EGF-receptor. In another embodiment, a method for treating a solid tumor characterized by overexpression of EGF receptor is disclosed including administering an agent that binds to the extracellular domain (ECD) of EGF receptor. The agent is selected from the group consisting of (a) an isolated polypeptide having from about 50 to 79 amino acids taken from the sequence of SEQ ID NO. 1, wherein the polypeptide binds to the extracellular domain ECD of EGF receptor at an affinity of at least 10 8 , (b) an isolated and glycosylated polypeptide having from about 80 to 419 amino acids taken from the sequence of SEQ ID NO. 2, wherein the C terminal 79 amino acids are present, and wherein at least three N-linked glycosylation sites are present, (c) a monoclonal antibody that binds to the ECD of EGF receptor, and (d) combinations thereof. The agent cannot be the monoclonal antibody alone. In yet another embodiment, a pharmaceutical composition for treating solid tumors that overexpress EGF receptor is disclosed including an agent selected from the group consisting of (a) an isolated polypeptide having from about 50 to 79 amino acids taken from the sequence of SEQ ID NO. 1, wherein the polypeptide binds to the extracellular domain ECD of EGF receptor at an affinity of at least 10 8 , (b) an isolated and glycosylated polypeptide having from about 80 to 419 amino acids taken from the sequence of SEQ ID NO. 2, wherein the C terminal 79 amino acids are present, and wherein at least three N-linked glycosylation sites are present, (c) a monoclonal antibody that binds to the ECD of EGF receptor, and (d) combinations thereof. The agent cannot be the monoclonal antibody alone, and pharmaceutically acceptable carrier. An even further embodiment, a method for targeting a therapeutic agent to solid tumor tissue is disclosed. The method includes targeting a therapeutic agent to a solid tumor wherein the solid tumor tissue is characterized by overexpression of EGF receptor. The method includes attaching the therapeutic agent to an isolated polypeptide having from about 50 to 79 amino acids taken from the sequence of SEQ ID NO. 1. The polypeptide binds to the extracellular domain ECD of EGF receptor at an affinity of at least 10 s . In yet another embodiment, a method for determining the prognosis of tumor treatment in a patient for a tumor that overexpresses EGF receptor is disclosed including : (a) obtaining a bodily fluid sample from a patient, wherein the bodily fluid is selected from the group consisting blood, serum, urine, lymph, saliva, tumor tissue, placental tissue, umbilical cord tissue, amniotic fluid, chorionic villi tissue and combinations thereof; (b) measuring the amount of p68HER-2 receptor expressed using an anti- p68HER-2 receptor antibody-based assay, wherein the assay is selected from the group consisting of ELISA, immunoprecipitation, immunohistocytochemistry, and Western analysis, (c) measuring the amount of EGF receptor ECD in the bodily fluid, and (d) determining a ratio between the amount of p68HER-2 and EGF receptor, whereby the higher the p68HER-2 to EGF receptor ratio, the better the prognosis of the patient. One advantage of herstatin is in the development of therapeutics, particularly against human cancers that involve group I receptor tyrosine kinases. Another advantage of herstatin is its ability to suppress the growth of pl85HER-2 or EGF receptor overexpressing cells. Brief Description of the Drawings Figure 1 shows a nucleotide and amino acid sequence of the insert in the extracellular domain of HER-2. The HER-2 ECD coding sequence from exon 1-9 (primers A and B) was amplified by PCR from a cDNA library from SKOV-3 cells. A product of ~1420 bp was found to be HER-2-specific by Southern blot analysis. This product was subcloned and the nucleotide sequence was determined. In panel A, the nucleotide sequence is shown for the 274 bp insert (outside the box) and for the immediately adjacent 5' and 3' sequences enclosed in the box. The insert is located between nucleotide residues 1171 and 1172 and following amino acid residue 340 in pl85HER-2 using the numbering of Coussens et al. (Science 230:1132-1139, 1985). The consensus 5' and 3' splice sites at the aπows are shown in larger print. The inserted sequence is in-frame with 5' HER-2 exon sequence and is deduced to encode a 79 amino acid extension following Arg 340 (R 340 ). The novel 79 novel amino acid sequence encoded by the insert is proline-rich (19%) and has a consensus asparagine linked glycosylation site, which is underlined. A stop codon was found at nucleotides 236-238 within the inserted sequence. In panel B, the predicted product of the alternative transcript is a truncated secreted protein which contains subdomains I and II identical to pl85 and is missing the transmembrane domain and cytoplasmic domain. If fully glycosylated, the expected size is 65-70 kDa. This polypeptide product is refeπed to as ρ68HER-2. Thus, the product will be a truncated secreted protein which is missing the transmembrane domain and cytoplasmic domain found in pl85HER-2. Figure 2 shows the detection of alternative HER-2 transcripts containing the ECDIIIa sequence by Northern blot analysis. PolyA+ mRNA (2.5 μg) from different human fetal tissues (Clontech) or isolated from HEK-293 cells was resolved in a formalin agarose gel and transfeπed to a BrightStar® membrane (Ambion) in lOxSSC. The membrane was hybridized with a 32 P-labeled antisense RNA probe complimentary to the ECDIII sequence, stripped and reprobed with a 32 P-labeled cDNA probe specific for the 5' HER-2 exon sequence. The membranes were washed under high stringency conditions and analyzed by phosphorimaging (Molecular Dynamics). Figure 3 shows a sequence-specific reactivity of anti-ECDIIIa with a protein of ~ 68 kDa in a human embryonic kidney cell line (HEK293). Cell extract protein (20 μg) and 20 μl of media conditioned by HEK-293 cells were Western blotted and probed with anti-ECDIIIa diluted 1:10,000 (lanes 1 and 2) or with anti-ECDIIa diluted 1:10,000 containing 50 μg/ml purified His-tagged ECDIIIa peptide (lanes 3, 4). Figure 4 shows the expression of pl85HER-2, relative to p68ECDIIIa expression, is markedly elevated in carcinoma cell lines in which the HER-2 gene is amplified. Cell extracts (15 μg of protein) from human embryonic kidney cell line (HEK293), nontumorigenic ovarian surface epithelial cell line (IOSEVAN), ovarian carcinoma cell line with HER-2 gene amplification (SKOV-3), nontumorigenic breast epithelial cell line (HBL100), and breast carcinoma cell lines with HER-2 gene amplification (BT474 and SKBR-3), were resolved by SDS-PAGE in 7.5% acrylamide gels and analyzed as a Western blot. The Western blot was probed with both antibodies specific for p68HER-2 (anti-ECDIIIa) and for p 185HER-2 (anti-neu(C)). Figure 5 shows that p68ECDIIIa binds to pl85HER-2. In panel A: Two mg of SKBR-3 cells extracted in nondenaturing buffer were immunoprecipitated with 5μl anti- neu(N) specific for the N-terminal sequence of p68HER-2 and pl85HER-2, or with 5 μl anti-neu(C) specific for the C-terminus of pl85HER-2 and then probed as a Western blot with both anti-ECDIIIa specific for p68HER-2 and with anti-neu(C) specific for pl85HER-2. In panel B: 100 μg of 17-3-1 cell extract were incubated in duplicate with 50 μl packed volume of NiNTA agarose (Qiagen) coupled to 20 μg of His-tagged ECDIIIa or to 20 μg His-tagged CREB fragment in 200 μl of wash buffer (20mM Tris pH 8.0, 300mM NaCl) at room temperature for 1 hr with shaking. The resin was then washed 4 times with 500 μl of wash buffer and proteins were eluted by incubation with 50 μl SDS-sample buffer at 100° C for 2 min. Eluted proteins were analyzed by Western blot analysis using antibodies against the C-terminus of pl85HER-2, anti-neu(C). In panel C: Monolayers of ~10 5 3T3 cells or HER-2 transfected 17-3-1 cells in 12 well plates were washed twice with PBS and then incubated with 0.5 ml of serum-free media with 1% BSA and 39, 75, 150, and 300 nM of purified recombinant His-tagged ECDIIIa for 2 hrs at 4 °C. Cells were washed 1 time in PBS containing 1% BSA and twice in PBS and then were extracted in denaturing buffer. Equal aliquots (20 μg protein) were analyzed by western blotting with antibodies specific for ECDIIIa (anti-ECDIIIa) or, in the upper panel, with antibodies specific forpl85HER-2 (anti-neu(C)). Figure 6 shows that neither p68-rich conditioned media nor the ECDIIIa peptide stimulate tyrosine phosphorylation of pl85HER-2. Monolayer cultures of ~10 5 HER-2 transfected 17-3-1 cells were washed twice with PBS, incubated in serum-free media at 37 °C for 24 hrs, and then treated for 10 minutes with 75 or 150 μM His-tagged ECDIIIa or with 50X CM from HEK-293 cells that secrete high levels of p68 or 50X CM from SKOV-3 cells that have no detectible p68HER-2. The treated cells were extracted with denaturing buffer containing the phosphotyrosine phosphatase inhibitor vanadate (2 mM) and 20 μg/ml of cell extract protein from each sample were analyzed by Western blot analysis with monoclonal antibodies against phosphotyrosine (Sigma). The blot was stripped by incubation at 55° C for 30 min in 62.5 mM Tris pH 6.7, 2% SDS, and 100 mM 2 -mercaptoethanol and then reprobed with anti-neu(C) specific for pl85HER-2. Figure 7 shows that p68HER-2 inhibited anchorage independent growth of tumorigenic cells. SKOV-3 ovarian cancinoma cells and HER-2 transfected 17-3-1 cells were suspended in media with 10%) fetal bovine serum containing 0.3% agar (control conditions) to which was added 5 OX concentrated media conditioned by SKOV-3 cells (which contains no detectable p68HER-2 (-p68 CM)), or 50X concentrated media conditioned by HEK-293 cells (which contains 20 nM p68HER-2 (+p68 CM)). Five times 10 3 cells were plated in triplicate for each experimental condition onto a 0.5 ml layer of media containing 0.5%ι agarose in 12 well plates. The results shown are plotted as the mean and standard deviation of the number of colonies with more than 50 cells in triplicate wells counted at 21 days of incubation. Similar results were observed in three separate experiments. Figure 8 shows the nucleotide and deduced amino acid sequence of HER-2 Intron 8. Human genomic DNA was subjected to PCR using primers that flank intron 8. PCR parameters were 30 cycles of 94 °C for 1 min, 62 °C for 1 min, 72 °C for 30 s, followed by 1 cycle of 72 °C for 7 min. A 410 bp product was gel purified and sequence in the forward an reverse directions. The sequence shown is the most common sequence found within intron 8 from about 15 different individuals. The positions of sequence variation that would result in amino acid substitutions are marked by Xs. Figures 9 and 10 show the expression of herstatin in combination with pl85HER-2 in transfected Cos-7 cells. Cos-7 cells were plated at 2 x 10 5 cell/well into 6-well plates and transfected using Lipofectamine (BioRrad) as described in Materials and Methods. The cells were transfected with 1.5 μg of herstatin expression vector plus 1.5 μg of HER-2 expression vector, or with 1.5 μg of HER-2 expression vectors (all pcDNA3.1 from Invitrogen). In each case the total amount of DNA was adjusted to 3 ug with control empty vector. In Fig. 9, upper panel, the transfected cells were analyzed at 48 hrs as a Western blot and reacted with antibodies against pl85HER-2, anti-neu (C), or in the lower panel with antibodies against the intron 8-encoded C-terminal sequence of herstatin (anti-Hst). In Fig. 10, the transfections were conducted in triplicate using the indicated plasmids with the inclusion of 0.5 μg of -galactosidase expression plasmid driven by a CMV promoter. At 48 hrs, the cells were extracted, cell protein was quantitated by BioRad protein dye kit, and the β-galactosidase activity was measured as described. The β-galactosidase activity was normalized to protein amounts and the mean results with the standard deviations are plotted. A similar result was obtained when β-galactosidase activity was normalized to number of cells originally plated into each well. Figures 11 and 12 show the effects of herstatin on the growth of transfected cell colonies. CHO cells were seeded at 2 x 10 5 cells/well in 6-well plates and triplicate wells were transfected as described in Materials and Methods with 3 μg of control empty plasmid (pcDNA 3.1; Invitrogen), 1.5 μg of pl85HER-2 expression plasmid plus 1.5 μg of control DNA; 1.5 μg of pl85HER-2 plus 1.5 μg of herstatin; or 1.5 μg of herstatin plus 1.5 μg of control DNA. At 48 hours after addition of DNA, the cells were trypsinized and diluted 1:10 into 6-well plates in the presence of 0.6 mg/ml of G418. The media was changed every two days. At 14 days, the plates were stained with crystal violet and washed. In the upper panel, the crystal violet stained plates were extracted by shaking at room temperature for 30 minutes with 1ml of 0.1 M NaPhosphate pH 4.5 in 50%) ethanol. The extracted crystal violet, diluted 10-fold, was quantitated by the absorbance at 515 nM. Dilutions of 10-fold gave readings of 0.1 to 1.0, which was in the linear range of absorbance versus cell number determined in pilot studies. Figures 13, 14 and 15 show that herstatin expression inhibits pl85HERτ2 tyrosine phosphorylation in transfected cells. Cos-7 cells were plated into 6-well plates and transfected as in Figures 9 and 10. In Figure 13, cells in duplicate wells were transfected with 0.25, 0.5, 1.0, and 3 μg of fluorescent green protein (FGP) expression plasmid. Empty vector was added to make the total amount of DNA equal to 3 μg in each well. At 48 hrs, the flourescent signal was quantitated at a wavelength of —for emission and ~ for excitation using a fluorescent plate reader. In Figures 14 and 15, cells were transfected with 0.5 μg of FGP plasmid with or without 1.5 μg of HER-2 plasmid, and with the indicated amounts of herstatin expression plasmid. Empty vector was added to make the total amount of DNA at 5 μg in each well. At 48 hrs, the cells were washed twice with PBS, extracted in 100 μl of modified RIP A containing ImM phenylmethylsulfonylfluoride and 2 mM orthovanadate. The protein concentration in the clarified extracts was determined using the BioRad protein dye kit. The extracted protein, 20 μg, was resolved using a 7.5%> polyacrylamide SDS gel and analyzed as a Western blot (Materials and Methods) first using 1 μg/ ml of anti-phosphotyrosine antibody (anti-PTyr) (Figure 15). The blot was stripped and then was reacted with antibodies against pl85HER-2 (anti-neu(C); Figure 14). The blots were developed using chemiluminescent reagent (Pierce) and exposed to Kodak film. Figures 16, 17, and 18 show that herstatin expression inhibits EGF activation of the EGF receptor in transfected cells. CHO cells at 2 x 10 5 cells/well were plated into 6- well plates and 24 hrs later duplicate wells were transfected with 0.5 μg of FGP marker plasmid with or without 1.5 μg of EGF receptor expression plasmid with the indicated amounts of herstatin expression plasmid. At 24 hours, the cells were washed twice with PBS and cultured in serum free media for an additional 24 hrs. In Figures 16 and 17 the cultures were incubated in the absence or presence of 100 ng/ml of EGF for 20 minutes and then extracted in modified RIP A as in Figures 13-15. 20 μg of protein from each well were resolved using 7% polyacrylamide SDS-gels and analyzed as a Western blot, first with 1 μg/ml of anti-phosphotyrosine antibody (Figure 17). The blot was then stripped and probed with anti-EGF receptor antibody (Figure 18). In Figure 18, the cultures were incubated in the absence or presence of 100 ng/ml of EGF for 24 hrs. The cell extracts were then analyzed as a Western blot using anti-EGF receptor antibody. Figure 19 shows the effects of the EGF receptor alone, and in combination with herstatin, on colony growth. CHO cells were plated in 6-well plates and triplicate wells were transfected with 1.5 μg of empty vector, with 1.5 μg of the EGF receptor expression plasmid, with 1.5 μg of the EGF receptor plus 1.5 μg of herstatin plasmid, or with 1.5 μg of herstatin plasmid. Empty vector was added to bring the total amount of DNA to 3 μg in each well. At 48 hrs after DNA addition, the cells in each well were collected by trypsinization and diluted 1:10 into 6-well plates in media containing 600 μg/ml of G418. The media was changed every two days and at 14 days, the cultures were stained with crystal violet. The dye from the stained cultures was extracted as in Figure 1 and quantitated by absorbance at 415 nM. The mean value of triplicate wells was determine as the % of the confrol transfected wells and the standard deviation are plotted. A simlar result was obtained in three separate experiments. Figure 20 shows that immobilized intron 8-encoded peptide or herstatin "pulls- down" the EGF receptor and pl85HER-2. About 100 μl of a 50 %> suspension of S- protein agarose (Novagen) was incubated with no peptide, with 50 μg of TBpexl4 peptide (provided by Dr. B. Ullnian, OHSU), 50 μg of intron 8-encoded peptide, or 50 μg full length recombinant herstatin at room temperature for lhr. Each of these peptides contained an S-protein tag encoded by the pET 30 expression plasmid (Novagen). The agarose samples were then washed twice with PBS and incubated at room temperature for 1 hr with 100 μg of A431 cell extract (for the EGF receptor) or 17-3-1 extract (for pl85HER-2), solubilized in PBS containing 1% nonidet-p40 (PBSNP-40). After incubation with the cell exfracts, the agarose samples were washed twice with 500 μl of PBS-NP40 and the proteins associated with the resin were eluted at 92°C for 2 min in 40 μl of SDS-sample buffer. To ensure that equal amounts of the original peptides were complexed to the agarose, an aliquot extracted in SDS-sample buffer was analyzed by SDS-PAGE and Coomassie staining of a 17% polyacrylamide gel for TBpexl4 and intron 8-encoded peptide, and alO %> polyacrylamide gel for p50 herstatin. To analyze receptor binding, an aliquot eluted from the agarose was analyzed as a Western blot using anti-EGFR or anti-pl85HER-2. Figure 21 shows, according to the present invention, that herstatin inhibited the EGF receptor-mediated stimulation of activated phospho-akt by EGF. The upper panel shows strong stimulation of the activated phospho-akt at 20 minutes after stimulation of the EGF receptor with EGF. In the presence of herstatin, the activated Akt signal was eliminated. The lower panel is a control demonstrating that the overall level of the Akt protein was constant. This result demonstrates the efficacy of herstatin in blocking the cell survival signal generated by EGF. Figure 22 shows, according to the present invention, that both TGFα-mediated stimulation of EGF receptor activation (upper panel), and TGFα-meidated stimulation of the Akt survival signal (lower panel), were strongly inhibited by herstatin. Figure 23 shows that stimulation of DNA synthesis by TGFα was blocked by herstatin expression. Cells that express herstatin, or control cells were treated with different concentrations of TGFα, and tested for DNA synthesis by measuring the amount of tritiated thymidine incorporation as a percentage of confrol values ("Normalized CPM"). Figure 24 shows that growth and/or survival of an ovarian carcinoma cell line (SKOV-3) and a breast carcinoma cell line (SKBR-3) that over-express HER-2 was inhibited by about 60%> relative to non-herstatin treated cells (left-most two bars). By contrast, the normal breast cell line (HBL-100) and the MCF-7 breast carcinoma cell line that express low levels of HER-2, were not significantly inhibited by purified recombinant herstatin (right-most two bars). Figure 25 illustrates that inhibition of growth and/or survival (as measured by optical density at 600 nM) of SKOV-3 carcinoma (left-most three bars), but not normal HBL-100 cells (right-most three bars), showed a dose response to purified recombinant herstatin. Figure 26 shows the analysis of recombinant herstatin in the blood of mfraperitoneally injected Nude mice. The samples in the upper panel were analyzed under non-reducing conditions by Western blot analysis using a monoclonal antibody against herstatin (Upstate Biologicals). In the lower panel, the samples were analyzed under under reducing conditions with a polyclonal antibody that reacts with multiple epitopes of herstatin (Doherty et a., PNAS, 1999). The presence of herstatin is detectable at 30 min and 1 hr after injection, but not at 3 hrs and 18 hrs after injection. Significantly, herstatin is not proteolytically degraded in the blood, because no smaller products of herstatin were detected in either of the Western blots. DETAILED DESCRIPTION OF THE INVENTION The present invention is based upon the initial discovery of an alternative HER-2 mRNA of 4.8 kb with a 274 bp insert identified as intron 8. The retained intron is in- frame and encodes 79 amino acids [SEQ ID NO. 1] followed by a stop codon at nucleotide 236. The alternative mRNA predicts a truncated HER-2 protein that lacks the transmembrane and intracellular domains and contains 419 amino acids [SEQ ID NO. 2]; 340 residues that are identical to the N-terminus of pl85HER-2 and 79 unique residues at the C-terminus [SEQ ID NO. 1]. Using specific antibodies against either the novel 79 amino acid residue C-terminal sequence [SEQ ID NO. 1] or the N-terminus of pl85HER- 2, a 68 kDa protein product was identified [SEQ ID NO.2]. This 68 kDa protein is the product of an alternative HER-2 transcript, and is found in cell extracts and in extracellular media from several cell lines. Expression of the alternative transcript was highest in a nontransfected human embryonic kidney cell line. The results presented here show expression of alternative HER-2 mRNA, which contains an additional 274 nucleotides, probably intron 8. Consistent with this finding, an alternative transcript of ~ 4.8 kb was detected in human fetal kidney tissue and in the human embryonic kidney cell line, HEK 293. Moreover, a transcript of 2.6 kb, which is the size expected if the sequence is retained in the 2.3 kb truncated HER-2 mRNA (Yamamoto et al., Nature 319:230-234, 1986; and Scott et al., Mol. Cell. Biol. 13:2247- 2257, 1993), was detected in human fetal liver tissue by Northern blot analysis using a probe specific for the inserted sequence or for the HER-2 ECD coding sequence (Figure 2). The inserted sequence introduces a termination codon and predicts a novel 79 amino acid extension designated ECDIIIa at residue 340 of the pl85HER-2 protein. The predicted protein therefore lacks the transmembrane and intracellular domains, but contains subdomains I and II of the extracellular domain of pl85HER-2. As predicted, a secreted protein that contains N-terminal sequence of pl85HER-2 and the C-terminal extension provided by the inclusion of the novel sequence was detected (Figures 3 and 5). The ECDIIIa protein was found to be 68 kDa which is the approximate size expected of the protein encoded by the alternative transcript if the five N-linked glycosylation sites found in subdomains I and II of pl85HER-2 are glycosylated (Stern et al., Mol. Cell. Biol. 6:1729-1740, 1986). The data presented herein demonstrate that p68HER-2 specifically binds to pl85HER-2. The association with pl85HER-2 may be confeπed by the novel proline rich ECDIIIa domain rather than the N-terminal subdomains I and II of p68HER-2. While the HER-2 ECD, generated by in vitro deletion mutagenesis, also contains subdomains I and II, it does not associate with the extracellular domain of pl85HER-2 unless engineered to enhance their proximity (Tzahar et al., EMBO J. 16:4938-4950, 1997; O'Rourke et al., Proc. Natl. Acad. Sci. USA 94:3250-3255, 1997; and Fitzpatrick et al., FEBS Letters 431:102-106, 1998). However, the unique ECDIIIa peptide binds with high affinity (nM concentrations) to pl85HER-2 and to transfected 17-3-1 cells that overexpress pl85HER-2 (Figure 5). Preferential binding of the ECDIIIa domain peptide to 17-3-1 cells indicates that secreted p68HER-2 interacts with the extracellular region of pl85HER-2 at the cell surface. Therefore, p68HER-2 and fragments thereof appear to be a naturally occurring HER-2 binding protein, encoded by the HER-2 gene. In contrast to EGFR family ligands (Groenen et al., Growth Factors 11:235-257, 1994), p68HER-2 lacks an EGF homology domain and contains the first 340 amino acids of the receptor itself, pl85HER Previously described putative HER-2 ligands were found to associate indirectly with pl85HER-2 only in a heterodimer with an EGFR family member (Heldin and Ostman, Cytokine Growth Factor Rev. 7:33-40, 1996). Although it is possible that ECDIIIa binds indirectly to pl85HER-2 through a coreceptor, this seems unlikely since detergent solubilized pl85HER-2 was specifically and efficiently "pulled down" by immobilized ECDIIIa peptide (Figure 5B). For all naturally occurring or engineered ligands for mammalian EGFR family members, binding is tightly coupled to stimulation of receptor dimerization and tyrosine phosphorylation (Hynes and Stem, Biochim. et Biophys. Acta 1198:165-184, 1994; Dougall et al., Oncogene 9:2109-2123, 1994; and Groenen et al., Growth Factors 11 :235-257, 1994). Although they bind, neither p68HER-2 nor the ECDIIIa peptide was found to activate pl85HER-2. Activation was assessed in two different cell lines that differ in the extent of pl85HER-2 tyrosine phosphorylation, transfected 17-3-1 cells as well as SKOV-3 ovarian carcinoma cells. Furthermore in vitro self-phosphorylation activity, which is enhanced in dimeric forms of pl85HER-2 (Dougall et al., Oncogene 9:2109-2123, 1994; and Lin et al., J Cell. Biochem. 49, 290-295, 1992), was not stimulated by p68HER-2 or ECDIIIa. Similarly, the Argos protein, which is an extracellular inhibitor of the Drosophila EGF receptor and the only known antagonist of class I RTKs, did not simulate tyrosine phosphorylation of the receptor (Schweitzer et al., Nature 376:699-702, 1995). Likewise, Angiopoietin-2, a natural antagonist for the Tie 2 RTK, bound the endothelial receptor but failed to activate it (Maisonpieπe et al., Science 277:55-60, 1997). Without being bound by theory, since p68HER-2 occupies but does not activate, it could block dimerization of pl85HR-2. By analogy, HER-2 ECD, when engineered to enhance its binding to RTKs, prevented the formation of productive dimers required for transphosphorylation and receptor activation thereby having a dominant negative effect (O'Rourke et al, Proc. Natl. Acad. Sci. USA 94:3250-3255, 1997). In contrast to the HER-2 ECD, soluble p68HER-2 exhibited strong binding to p 185HER-2, yet also contains subdomain I and II of the ECD. Since subdomain I may be the low affinity, promiscuous ligand binding site required for recruitment of pl85HER-2 into heteromeric complexes (Tzahar et al, EMBO J. 16:4938-4950, 1997), p68HER-2 could block this site and thereby obstruct recruitment of pl85HER-2 into dimers. Alternatively, p68HER-2 could compete with an uncharacterized ligand for binding to p 185HER-2. The tissue- specific expression of p68HER-2 in human fetal liver and kidney may function to modulate the extent to which pl85HER-2 is occupied during development of these organs. Moreover, the overexpression of pl85HER-2, relative to p68HER-2 in tumor cells with HER-2 gene amplification (Figure 3), could occur though a selective pressure based on overcoming the effects of a binding protein such as p68HER-2. Therefore, p68HER-2 is the first example of a naturally occurring pl85HER-2 binding protein that may prevent activation of pl85HER-2. Pharmaceutical Composition The present invention further provides a pharmaceutical composition for treating solid tumors that overexpress HER-2, comprising an agent selected from the group consisting of (a) an isolated polypeptide having from about 50 to 79 amino acids taken from the sequence of SEQ ID NO. 1, wherein the polypeptide binds to the extracellular domain ECD of HER-2 at an affinity of at least 10 s , (b) an isolated and glycosylated polypeptide having from about 300 to 419 amino acids taken from the sequence of SEQ ID NO. 2, wherein the C terminal 79 amino acids are present, and wherein at least three N-linked glycosylation sites are present, (c) a monoclonal antibody that binds to the ECD of HER-2, and (d) combinations thereof, with the proviso that the agent cannot be the monoclonal antibody alone, and pharmaceutically acceptable carrier. Preferably, the agent is the isolated polypeptide having from about 50 to 79 amino acids taken from the sequence of SEQ ID NO. 1. Most preferably, the agent is a combination of the isolated polypeptide having from about 50 to 79 amino acids taken from the sequence of SEQ ID NO. 1 and the monoclonal antibody that binds to the ECD of HER-2. The inventive pharmaceutical composition, comprising either or both of the inventive polypeptides and/or monoclonal antibody, can be administered to a patient either by itself (complex or combination) or in pharmaceutical compositions where it is mixed with suitable carriers and excipients. Inventive polypeptide can be administered parenterally, such as by intravenous injection or infusion, intraperitoneal injection, subcutaneous injection, or intramuscular injection. Inventive polypeptide can be administered orally or rectally through appropriate formulation with carriers and excipients to form tablets, pills, capsules, liquids, gels, syrups, slurries, suspensions and the like. Inventive polypeptide can be administered topically, such as by skin patch, to achieve consistent systemic levels of active agent. Inventive polypeptide is formulated into topical creams, skin or mucosal patch, liquids or gels suitable to topical application to skin or mucosal membrane surfaces. Inventive polypeptide can be administered by inhaler to the respiratory tract for local or systemic treatment of cancers characterized by overexpressing HER-2. The dosage of inventive polypeptide suitable for use with the present invention can be determined by those skilled in the art from this disclosure. Inventive polypeptide will contain an effective dosage (depending upon the route of administration and pharmacokinetics of the active agent) of inventive polypeptide and suitable pharmaceutical carriers and excipients, which are suitable for the particular route of administration of the formulation (i.e., oral, parenteral, topical or by inhalation). The active inventive polypeptide is mixed into the pharmaceutical formulation by means of mixing, dissolving, granulating, dragee-making, emulsifying, encapsulating, entrapping or lyophilizing processes. The pharmaceutical formulations for parenteral administration include aqueous solutions of the inventive polypeptide in water-soluble form. Additionally, suspensions of the inventive polypeptide may be prepared as oily injection suspensions. Suitable lipophilic solvents or vehicles include fatty oils such as sesame oil, or synthetic fatty acid esters, such as ethyl oleate or triglycerides, or liposomes. Aqueous injection suspensions may contain substances which increase the viscosity of the suspension, such as sodium carboxymethyl cellulose, sorbitol, or dextran. The suspension may optionally contain stabilizers or agents to increase the solubility of the complex or combination to allow for more concentrated solutions. Pharmaceutical formulations for oral administration can be obtained by combining the active compound with solid excipients, such as sugars (e.g., lactose, sucrose, mannitol or sorbitol), cellulose preparations (e.g., starch, methyl cellulose, hydroxypropylmethyl cellulose, and sodium carboxymethyl cellulose), gelaten, gums, or polyvinylpyrrohdone. In addition, a desmtegrating agent may be added, and a stabilizer may be added. Processes for Synthesizing p68 and 79 aa C Terminal Region Polypeptide synthesis is done by a group of standard procedures for polypeptide synthesis by sequential amino acids building through peptide synthesis equipment, following manufacturer's instructions for synthesizing peptides. Preferably, shorter polypeptides, of less than 100 amino acids, are best suited for the method of synthesis through sequential amino acid building of polypeptides. In addition, heterologous polypeptides can be expressed by transformed cells using standard recombinant DNA techniques to transform either prokaryotic or eukaryotic cells, provide appropriate growth media for their expression, and then purify the inventive polypeptide either from the media or from intracellular contents depending upon the type of cell used and its expression characteristics. Methods for Treating Cancer with p68. 79 aa C Terminal Region, and Combinations The present invention provides a method for treating a solid tumor characterized by overexpression of HER-2, or HER-2 variants (see Example 8) comprising administering an agent that binds to the extracellular domain (ECD) of HER-2, wherein the agent is selected from the group consisting of (a) an isolated polypeptide having from about 50 to 79 amino acids taken from the sequence of SEQ ID NO. 1, wherein the polypeptide binds to the extracellular domain ECD of HER-2 at an affinity of at least 10 s , (b) an isolated and glycosylated polypeptide having from about 300 to 419 amino acids taken from the sequence of SEQ TD NO. 2, wherein the C terminal 79 amino acids are present, and wherein at least three N-linked glycosylation sites are present, (c) a monoclonal antibody that binds to the ECD of HER-2, and (d) combinations thereof, with the proviso that the agent cannot be the monoclonal antibody alone. Preferably, the solid tumor that overexpresses HER-2 is selected from the group consisting of breast cancer, small cell lung carcinoma, ovarian cancer, prostate cancer, gastric carcinoma, cervical cancer, esophageal carcinoma, and colon cancer. Preferably, the agent is the isolated polypeptide having from about 50 to 79 amino acids taken from the sequence of SEQ ID NO. 1. Most preferably, the agent is a combination of the isolated polypeptide having from about 50 to 79 amino acids taken from the sequence of SEQ ID NO. 1 and the monoclonal antibody that binds to the ECD of HER-2. The p68HER-2 polypeptide described herein was found to bind to HER-2 and prevent signal transduction through the kinase domain. Without being bound by theory, the unique ECDIIIa domain mediates specific binding to p 185HER-2 and the resulting interaction with p68ECDIIIa prevents pl85HER-2 dimerization and subsequent signal transduction. Therefore, p68HER-2 functions as a HER-2 antagonist to prevent signal transduction by preventing dimerization as a necessary prerequisite for signal transduction. Thus, the mechanism of p68HER-2 as a HER-2 antagonist is different from the mechanism of binding agents, such as the 79 amino acid polypeptide described herein or a monoclonal antibody that binds to the EDC of HER-2. The inventive method provides that p68HER-2 inhibits tumor cell growth in tumors that overexpress HER-2 by providing a selective pressure for such tumor cells. Similarly, the HER-2 antagonists that are binding agents also inhibit tumor cell growth in tumors that overexpress HER-2 by providing selective pressure to such cells to prevent ligand binding to the ECD of HER-2 and prevent signal transduction even before potential dimerization. Use of 79 aa C Terminal Region as a Targeting Molecule The present invention further provides a method for targeting a therapeutic agent to solid tumor tissue, wherein the solid tumor tissue is characterized by overexpression of HER-2, comprising attaching the therapeutic agent to an isolated polypeptide having from about 50 to 79 amino acids taken from the sequence of SEQ ID NO. 1, wherein the polypeptide binds to the extracellular domain ECD of HER-2 at an affinity of at least 10 s . Preferably, the isolated polypeptide is from about 69 to 79 amino acids in length. Preferably, the isolated polypeptide binds to a site on the ECD of HER-2 that is different from the site of binding of Herceptin® (a marketed humanized monoclonal antibody that is used for the treatment of cancer and that binds to the ECD or HER-2). It was discovered that the 79 amino acid polypeptide [SEQ ID NO. 1] exhibited surprising high affinity binding properties to the ECD of HER-2. Moreover, the site of such binding is different and unaffected by the site of binding of a marketed humanized monoclonal antibody (Herceptin®). Therefore, the high binding affinity enables the 79 amino acid polypeptide to function as a targeting molecule to tumor cells expressing HER-2. Anti-p68 Antibody as a Diagnostic/Prognostic Agent The p68HER-2 ECDIIIa variant 3 (see TABLE 1, below) glycosylated polypeptide was expressed and used as an antigen for antibody production. Specifically, antibody specific for p68HER-2 was prepared by injecting rabbits with purified polyhistidine-tagged ECDIIIa variant 3 peptide, which is the same as the intron encoded novel C-terminus or p68HER-2, the domain that binds with high affinity to pl85HER-2. The isolated polyclonal antibody detected pM quantities of ECDIIIa peptide or of p68HER-2 with high specificity (see Figures 3 and 5). Thus, an antibody specific for p68HER-2 is useful as a diagnostic agent for detecting p68HER-2 in bodily fluids and tumor tissues using diagnostic techniques, such as ELISA, immunoprecipitations, immunohistochemistry or Western analysis. Antibodies that specifically recognize one or more epitopes of ECDIIIa, or epitopes of p68HER-2, or peptide fragments, and thus distinguish among ECDIIIa variants (see TABLE 1, below) are also encompassed by the invention. Such antibodies include but are not limited to polyclonal antibodies, monoclonal antibodies (mAbs), humanized or chimeric antibodies, single-chain antibodies, Fab fragments, F(ab').sub.2 fragments, fragments produced by a Fab expression library, anti-idiotypic (anti-Id) antibodies, and epitope-binding fragments of any of the above. The antibodies of the invention may be used, for example, in the detection of a particular p68HER-2 ECDIIIa variant in a biological sample and may, therefore, be utilized as part ofa diagnostic or prognostic technique whereby patients or tissue samples may be tested for the presence of particular variants, or for abnormal amounts particular variants. Such antibodies may also be utilized in conjunction with, for example, compound screening schemes for the evaluation of the effect of test compounds on expression and/or activity of particular p69HER-2 variants. Additionally, such antibodies can be used in conjunction with the cancer treatment methods described herein. For the production of antibodies, various host animals may be immunized by injection with e.g., polyhistidine-tagged ECDIIIa variant polypeptides, truncated ECDIIIa variant polypeptides, functional equivalents of the ECDIIIa variants or mutants of the ECDIIIa region. Such host animals may include but are not limited to rabbits, mice, hamsters and rats, to name but a few. Various adjuvants may be used to increase the immunological response, depending on the host species, including but not limited to Freund's (complete and incomplete), mineral gels such as aluminum hydroxide, surface active substances such as lysolecithin, pluronic polyols, polyanions, peptides, oil emulsions, keyhole limpet hemocyanin, dinitrophenol, and potentially useful human adjuvants such as BCG (bacille Calmette-Guerin) and Corynebacterium parvum. Polyclonal antibodies are heterogeneous populations of antibody molecules derived from the sera of the immunized animals. Monoclonal antibodies, which are homogeneous populations of antibodies to a particular antigen, may be obtained by any technique that provides for the production of antibody molecules by continuous cell lines in culture. These include, but are not limited to, the hybridoma technique of Kohler and Milstein, (Nature 256:495-497, 1975; and U.S. Pat. 4,376,110), the human B-cell hybridoma technique (Kosbor et al, Immunology Today 4:72, 1983; Cole et al., Proc. Natl. Acad. Sci. USA 80:2026-2030, 1983), and the EBV-hybridoma technique (Cole et al., Monoclonal Antibodies And Cancer Therapy, Alan R. Liss, Inc., pp. 77-96, 1985). Such antibodies may be of any immunoglobulin class including IgG, IgM, IgE, IgA, IgD and any subclass thereof. Hybridomas producing mAb may be cultivated in vitro or in vivo. Production of high titers of mAbs in vivo makes this the presently prefeπed method of production. Additionally, techniques developed for the production of "chimeric antibodies" (Morrison et al., Proc. Natl. Acad. Sci. USA, 81:6851-6855, 1984; Neuberger et al., Nature, 312:604-608, 1984; Takeda et al., Nature, 314: 452-454, 1985) by splicing the genes from a mouse antibody molecule of appropriate antigen specificity together with genes from a human antibody molecule of appropriate biological activity can be used. A chimeric antibody is a molecule in which different portions are derived from different animal species, such as those having a variable region derived from a murine mAb and a human immunoglobulin constant region (humanized). Alternatively, techniques described for the production of single-chain antibodies (U.S. Pat. No. 4,946,778; Bird, Science 242:423-426, 1988; Huston et al., Proc. Natl. Acad. Sci. USA 85:5879-5883, 1988; and Ward et al., Nature 334:544-546, 1989) can be adapted to produce single-chain antibodies against ECDIIIa variant gene products. Single-chain antibodies are formed by linking the heavy and light chain fragments of the Fv region via an amino acid bridge, resulting in a single chain polypeptide. Antibody fragments that recognize specific epitopes may be generated by known techniques. For example, such fragments include but are not limited to: the F(ab').sub.2 fragments which can be produced by pepsin digestion of the antibody molecule and the Fab fragments which can be generated by reducing the disulfide bridges of the F(ab').sub.2 fragments. Alternatively, Fab expression libraries may be constructed (Huse et al., Science, 246:1275-1281, 1989) to allow rapid and easy identification of monoclonal Fab fragments with the desired specificity. Antibodies to particular ECDIIIa variants can, in turn, be utilized to generate anti- idiotype antibodies that "mimic" the ECDIIIa variant, using techniques well known to those skilled in the art. (Greenspan & Bona, FASEB J 7 (5):437-444, 1993; and Nissinoff, J. Immunol. 147:2429-2438, 1991). For example antibodies which bind to an ECDIIIa variant and competitively inhibit the binding of p68HER-2 to HER-2 receptor can be used to generate anti-idiotypes that "mimic" the ECDIIIa variant and, therefore, bind and neutralize HER-2 receptor. Such neutralizing anti-idiotypes or Fab fragments of such anti-idiotypes can be used in cancer therapeutic regimens. Alternatively, antibodies to particular ECDIIIa variants that can act as agonists or antagonists of the ECDIIIa variant activity can be generated. Such antibodies will bind to the ECDIIIa variant and modulate the activity of p68HER-2 vis-a-vis pl85HER-2 receptor-mediated signal transduction. Such antibodies may be particularly useful for treating particular cancers and/or modulating tumor differentiation. Accordingly, the present invention further provides a method for determining the prognosis of tumor treatment for a tumor that overexpresses HER-2, comprising: (a) obtaining a bodily fluid, wherein the bodily fluid is selected from the group consisting of blood, serum, urine, lymph, saliva, tumor tissue, and combinations thereof; and (b) measuring the amount of p68HER-2 expressed using an anti-p68HER-2 antibody-based assay, wherein the assay is selected from the group consisting of ELISA, immunoprecipitation, immunohistocytochemistry, and Western analysis. Preferably, the method for determining the prognosis of tumor treatment further comprises measuring the amount of pl85HER-2 ECD in the bodily fluid, and determining a ratio between the amount of p68HER-2 and pl85HER-2. The higher the ratio of p68HER-2:pl85HER-2, the better the treatment prognosis. ECDIIIa region Variants as Diagnostic/Prognostic Agents Example 11 (below) shows that the human sequence of intron 8 is both proline- rich and polymorphic. Sequencing of genomic DNA from fifteen different individuals resulted in the identification of 10 variable sequence regions within Her-2 Intron 8. See SEQ ID NO: 10; Figure 8, and Table 1. Figure 8 shows the most common nucleotide and coπesponding polypeptide sequences of intron 8. This region contains 10 different polymorphisms (marked by the letters W (2x), Y (3x), R, N, M, and S (2x) in SEQ ID NO: 10; or marked by an "X" in Figure 8) that result in nonconservative amino acid substitutions (see legend to TABLE 1). For example, the polymorphism (G — > C) at nucleotide position 161 (Figure 8; TABLE 1) would result in a substitution of Arginine (R) for Proline (P) at amino acid residue #54 of SEQ ID NO:l, or residue #394 of SEQ ID NO:2. The N-tenninal Glycine (G), designated as position 1 in Figure 8 or SEQ ID NO:10, coπesponds to amino acid residue 341 in the "herstatin" sequence (Doherty et al., Proc. Natl. Acad. Sci. USA 96:10,869-10,874, 1999). The nucleotide sequence shown in Figure 1(A) (Doherty et al, Proc. Natl. Acad. Sci. USA 96:10,869-10,874, 1999), is a polymorphic form that differs at amino acid residues #6 and #73 from the most commonly detected sequence shown here in Figure 8. This result demonstrates that in the human population there are several variations in the intron-8 encoded domain that could lead to altered biochemical and biological properties among ECDIIIa-containing protein variants. An individual may, inter alia, be genetically heterozygous for two variants, homozygous for a given variant, or homozygous for a double variant. Both tumor progression and optimal treatment may vary depending upon the particular variants represented in a given individual. This variability has both prognostic and diagnostic utility. The present invention shows that ECDIIIa-containing polypeptides can bind tightly to, and thus antagonize the HER-2 receptor. Such a specific, high-affinity interaction is dependent upon particular primary, secondary and tertiary structure of the ECDIIIa-containing polypeptide. The ECDIIIa region is proline-rich, and it is well known in the art that nonconservative substitution of proline residues, or other residues within a proline-rich sequence, in a given protein can have profound effects on its secondary and tertiary structure. Thus, the polymorphisms of the present invention are likely to embody significant structural, biochemical and biological differences relative to the most common polypeptide structure (shown in Figure 8). Structural differences among ECDIIIa variant proteins may include for example, differences in size, electronegativity, or antigenicity. Differences in biological properties among ECDIIIa variants might be seen e.g., in the relative degree of cellular secretion, the nature and/or extent of modulation of the HER-2 receptor, pharmacokinetics (e.g., serum half-life, elimination profile), resistance to proteolysis, N-linked glycosylation patterns, etc. These biological differences, in turn, would be expected to alter tumor progression and thus optimal treatment protocols. Thus, the knowledge that an individual contains a particular ECDIIIa variant or variants (e.g., in individuals heterozygous for a given variant, or individuals with compound variants like variant 11 of Table 1), may, in itself, be prognostic of particular cancer susceptibility. The apparent genetic heterogeneity of ECDIIIa region means that the nature of the particular ECDIIIa variation carried by an individual may have to be ascertained using sequence identity assays prior to attempting genetic diagnosis of the patient. The analysis can be caπied out on any genomic DNA derived from bodily fluids of the patient, typically a blood sample from an adult or child, but alternatively may be serum, urine, lymph, saliva, tumor tissue, placental tissue, umbilical cord tissue, amniotic fluid, and chorionic villi samples. It is expected that standard genetic diagnostic methods, such as hybridization or amplification assays, can be used. Either DNA or RNA, may, for example, be used in hybridization or amplification assays of biological samples to detect particular ECDIIIa variant sequences. Such sequence identity assays may include, but are not limited to, Southern or Northern analyses, single-stranded confoπnational polymorphism analysis, in situ hybridization assays, and polymerase chain reaction ("PCR") analyses. Such analyses may reveal both quantitative and qualitative aspects of ECDIIIa variant sequence expression. Such aspects may include, for example, point mutations, and/or activation or inactivation of gene expression. Standard in situ hybridization techniques may be used to provide information regarding which cells within a given tissue express a particular ECDIIIa variant sequence. Preferably, diagnostic methods for the detection of ECDIIIa variant nucleic acid molecules involve contacting and incubating nucleic acids, derived from cell types or tissues being analyzed, with one or more labeled nucleic acid reagents, or probes, specific for particular ECDIIIa variants. More preferably, PCR, or reverse transcription PCR, can be utilized to identify nucleotide variation within the ECDIIIa domain. PCR reaction conditions should be chosen which optimize amplified product yield and specificity, and, additionally, produce amplified products of lengths that may be resolved utilizing standard gel electrophoresis techniques. Such reaction conditions are well known to those of skill in the art, and important reaction parameters include, for example, length and nucleotide sequence of oligonucleotide primers, and annealing and elongation step temperatures and reaction times. Following the PCR reaction, the PCR products can be analyzed by methods such as heteroduplex detection, cleavage of RNA- DNA hybrids using Rnase A, single-stranded conformational polymorphisms, and denaturing gradient gel electrophoresis. Additionally, if the particular ECDIIIA sequence variant is known to add or remove a restriction site, or to have significantly altered the size of a particular restriction fragment, a protocol based upon restriction fragment length polymorphism ("RFLP") analysis may be appropriate. ECDIIIa variants can also be analyzed at the expression level using sequence identity assays with bodily fluids derived from the patient, typically a blood sample from an adult or child, but may include serum, urine, lymph, saliva, tumor tissue, placental or umbilical cord cells, amniotic fluid, and chorionic villi samples. Well-known sequence identity assays for analyzing expression include, but are not limited to, mRNA-based methods, such as Northern blots and in situ hybridization (using a nucleic acid probe derived from the relevant cDNA), and quantitative PCR (as described by St- Jacques et al, Endocrinology 134:2645-2657, 1994). Polypeptide-based methods (e.g., including but not limited to western blot analysis) including the use of antibodies specific for the ECDIIIa variant of interest, as discussed above, could also be used. These techniques permit quantitation of the amount of expression ofa given ECDIIIa variant, at least relative to positive and negative controls. Preferably, a battery of monoclonal antibodies, specific for different ECDIIIa eptitopes or variants, could be used for rapidly screening cells or tissue samples to detect those expressing particular ECDIIIa variants, or for quantifying the level of ECDIIIa variant polypeptides. Prefeπed diagnostic methods for the quantitative or qualitative detection of ECDIIIa variant peptide molecules may involve, for example, immunoassays wherein particular ECDIIIa-containing peptides are detected by their interaction with anti-ECDIIIa variant specific antibodies. This can be accomplished for example, by immunofluorescence techniques employing a fluorescently labeled antibody coupled with light microscopic, flow cytometric, or fluorometric detection. The antibodies (or fragments thereof) useful in the present invention may, additionally, be employed histologically, as in immunofluorescence or immunoelectron microscopy, for in situ detection of ECDIIIa-containing peptides. Through the use of such procedures, it is possible to determine not only the presence of particular ECDIIIa-containing polypeptides, but also their distribution in the examined tissue. Immunoassays for ECDIIIa variant polypeptides preferably comprise incubating a biological sample, such as the above-named bodily fluids, which have been incubated in the presence of a detectably labeled antibody capable of identifying ECDIIIa-containing peptides, and detecting bound antibody by any of a number of techniques well known in the art. The biological sample may be brought in contact with and immobilized onto a solid phase support or carrier such as nitrocellulose, or other solid support that is capable of immobilizing soluble proteins, cells, or cell particles. The support may then be washed with suitable buffers followed by treatment with the detectably labeled anti- ECDIIIa variant specific antibody. The solid phase support may then be washed with the buffer a second time to remove unbound antibody. The amount of bound label on the solid support may then be detected by conventional means. Alternatively, anti-ECDIIIa variant specific antibodies can be detectably labeled by linking the same to an enzyme for use in an enzyme immunoassay or Enzyme Linked Immunosorbent Assay ("ELISA"). The enzyme which is bound to the antibody will react with an appropriate substrate, preferably, a chromogenic substrate, in such a manner as to produce a chemical moiety which can be detected, for example, by spectrophotometric, fluorimetirc or by visual means. Enzymes which can be used to detectably label the antibody include, but are not limited to, malate dehydrogenase, staphylococcal nuclease, delta-5-steroid isomerase, yeast alcohol dehydrogenase, alpha- glycerophosphate, dehydrogenase, triose phosphate isomerase, horseradish peroxidase, alkaline phosphatase, asparaginase, glucose oxidase, beta-galactosidase, ribonuclease, urease, catalase, glucose-6-phosphate dehydrogenase, glucoamylase and acetylcholinesterase. The detection can be accomplished by calorimetric methods that employ a chromogenic substrate for the enzyme. Detection may also be accomplished visually by comparison of the extent of enzymatic reaction with appropriate standards. Detection may also be accomplished using any of a variety of other immunoassays. For example, by radioactively labeling the antibodies or antibody fragments, it is possible to detect ECDIIIa-containing peptides through the use of a radioimmunoassay (RIA). The radioactive isotope can be detected by such means as the use of a gamma counter or a scintillation counter or by autoradiography. It is also possible to label the antibody with a fluorescent compound. When the fluorescently labeled antibody is exposed to light of the proper wavelength, its presence can be detected due to fluorescence. Among the most commonly used fluorescent labeling compounds are fiuorescein isothiocyanate, rhodamine, phycoerythrin, phycocyanin, allophycocyanin, o-phthaldehyde and fluorescamine. The antibody can also be detectably labeled using fluorescence emitting metals such as 152 Eu, or others of the lanthanide series. These metals can be attached to the antibody using such metal chelating groups as diethylenetriaminepentacetic acid (DTP A) or ethylenediaminetefraacetic acid (EDTA). The antibody also can be detectably labeled by coupling it to a chemiluminescent compound. The presence of the chemiluminescent-tagged antibody is then determined by detecting the presence of luminescence that arises during the course of a chemical reaction. Examples of particularly useful chemiluminescent labeling compounds are luminol, isoluminol, theromatic acridinium ester, imidazole, acridinium salt and oxalate ester. Likewise, a bioluminescent compound may be used to label the antibody of the present invention. Bioluminescence is a type of chemiluminescence found in biological systems in which a catalytic protein increases the efficiency of the chemiluminescent reaction. The presence of a bioluminescent protein is determined by detecting the presence of luminescence. Important bioluminescent compounds for purposes of labeling are luciferin, luciferase and aequorin. The binding activity of a given lot of anti-ECDIIIa- variant specific antibody may be determined according to well-known methods. Those skilled in the art will be able to determine operative and optimal assay conditions for each determination by employing routine experimentation. Accordingly, the present invention, including the unexpected discovery ofa plurality of variable sequence positions within the proline-rich ECDIIIa region, along with antibodies specific for particular ECDIIIa variants, provides for valuable prognostic and diagnostic information and assays. Accordingly, the present invention further proivides a method for determining the prognosis of tumor treatment in a patient for a tumor that overexpresses HER-2, comprising: (a) obtaining a bodily fluid sample from the patient, wherein the bodily fluid is selected from the group consisting of blood, serum, urine, lymph, saliva, tumor tissue, placental tissue, umbilical cord tissue, amniotic fluid, chorionic villi tissue, and combinations thereof; and (b) measuring the amount of p68HER-2 expressed using an anti-p68HER-2 antibody-based assay, wherein the assay is selected from the group consisting of ELISA, immunoprecipitation, immunohistocytochemistry, and Western analysis. Preferably, the method for determining the prognosis of tumor treatment further comprises measuring the amount of pl85HER-2 ECD in the bodily fluid, and determining a ratio between the amount of p68HER-2 and pl85HER-2. The higher the ratio of p68HER-2:pl85HER-2, the better the treatment prognosis. Preferably, the method for determining the prognosis of tumor treatment further comprises determining which particular ECDIIIa variants are present and optimizing tumor treatment in view of particular biochemical and biological properties among ECDIIIa protein variants. The present invention further provides an assay for cancer treatment, prognosis or diagnosis in a patient comprising: (a) obtaining a bodily fluid sample from the patient, wherein the bodily fluid is selected from the group consisting of blood, serum, urine, lymph, saliva, tumor tissue, placental tissue, umbilical cord tissue, amniotic fluid, chorionic villi tissue and combinations thereof; (b) determining whether a particular ECDIIIa variant sequence is present in the bodily fluid sample with a sequence identity assay; and (c) coπelating the presence of the ECDIIIa variant sequence to cancer treatment and diagnosis using an historical database. Preferably, the sequence identity assay is selected from the group consisting of DNA sequencing, PCR assays, ELISA immunologic assays, immunoassays, hybridization assays, and combinations thereof. The present invention further provides an assay for cancer treatment, prognosis or diagnosis in a patient comprising: (a) obtaining a bodily fluid sample from the patient, wherein the bodily fluid is selected from the group consisting of blood, serum, urine, lymph, saliva, tumor tissue, placental tissue, umbilical cord tissue, amniotic fluid, chorionic villi tissue and combinations thereof; (b) determining whether an amount of an ρ68HER-2 ECDIIIa variant is present in the bodily fluid sample using an anti-p68HER-2 antibody-based assay, wherein the assay is selected from the group consisting of ELISA, immunoprecipitation, immunohistocytochemistry, and Western analysis; and (c) coπelating the presence or amount of the p68HER-2 ECDIIIa variant to cancer treatment and diagnosis using an historical database. The present invention further provides for the above-mentioned cancer treatment, prognostic or diagnostic assays, further comprising measuring the amount of pl85HER-2 ECD in the bodily fluid sample. The present invention further provides for the above-mentioned cancer treatment, prognostic or diagnostic assays further comprising measuring the amount of pl85HER-2 ECD in the bodily fluid sample, and determining a ratio between the amount of pl85HER-2 ECD and a particular p68HER-2 ECDIIIa variant. The present invention further provides for antibodies specific for ECDIIIa variants of the sequence in SEQ ID NO:l or SEQ ID NO:2, below. P68HER-2 as a Therapeutic Agent Without being bound by theory, but it appears that p68HER-2 or ECDIIIa peptide inhibits the growth of tumor cells that overexpress HER-2 by binding to pl85HER-2 at the cells surface. This hypothesis was examined by testing anchorage independent growth of cells in the presence or absence of p68HER-2 using cells that depend on pl85HER-2 overexpression for their malignant growth, yet have little or no detectable p68HER-2. Anchorage independent growth of cells in soft agar was used as a predictive model for tumor cytotoxicity. This is a common and predictive procedure to examine transforming activity and reflects the tumorigenic and oncogenic potential of cells (DiFore et al., Science 237:178-182, 1987; Hudziak et al., Proc. Natl. Acad. Sci. USA 84:7159-7163, 1987; and Baasner et al., Oncogene 13:901-911, 1996). The effects of p68HER-2 on anchorage independent growth in soft agar was determined using SKOV-3 carcinoma cells and HER-2 transfected 17-3-1 cells, which are both tumorigenic and overexpress pl85HER-2. The cells were suspended in media supplemented with fetal calf serum in the presence or absence of p68HER-2 and incubated for 21 days in a humidified incubator. Anchorage independent growth was quantitated by counting the number of colonies that contained more than 50 cells. Figure 7 shows that in the presence of p68HER-2, anchorage independent growth of both SKOV-3 cells and 17-3-1 cells was inhibited several fold. Accordingly, these data show that p68HER-2 is not just cytostatic, but cytotoxic and possibly apoptotic. Coexpression of herstatin inhibits p!85HER-2 levels and inhibits colony formation. The effects of ectopic herstatin expression on pl85HER-2 in transfected Cos-7 cells were examined. Figure 9 shows that as compared to cells transfected with HER-2 alone, pl85 levels were decreased about 5 fold when herstatin was coexpressed. If herstatin expression caused diminished survival of transfected cells, this may explain decreased pl85HER-2 levels in the cotransfected cells. Whether expression of the marker -galactosidase plasmid was altered in the cotransfected cells was examined. Fig. 10 illusfrates that the -gal activity was greatest when the -gal plasmid was cotransfected with pl85HER-2, which was about 35% higher than when cotransfected with control (pcDNA empty plasmid). When cells were cotransfected with herstatin in combination with pl85HER-2 and the -gal plasmid, the level of - gal activity was reduced about 3 fold. This reduction was observed in two addtional cell lines, CHO and HEK-293. Moreover, a similar level of inhibition was observed when expression of an alternative marker, fluorescent green protein (FGP), was examined in the cells transfected with herstatin and HER-2 (not shown). The results show that transfection with HER-2 alone increased the level of a cotransfected marker, while coexpression with herstatin and HER-2 reduced the marker signal. One possible explanation is that survival of the transfected cells is enhanced by expression of pl85HER-2 and is inhibited by coexpression of herstatin and pl85HER-2. To directly measure effects on colony formation, CHO cells were transfected, subjected to selection with G418 for 20 days, and the surviving cell colonies were stained and quantitated. CHO cells are well suited for rapid growth of stably transfected cells. As shown in Figre 11, transfection with pl85HER-2 enhanced colony growth by about 60% relative to the control transfected cells, while herstatin expressed alone did not significantly alter growth. However, cofransfection of herstatin dramatically reduced colony formation of HER-2 transfected cells by about 7-fold (Figure 11). Herstatin expression inhibits p!85HER-2 tyrosine phosphorylation The impact of herstatin expression on pl85HER-2 was further evaluated by varying the proportion of herstatin to p 185HER-2 expression plasmids. When the herstatin plasmid was introduced at five-fold lower levels relative to the pl85HER-2 plasmid, there was little effect on p 185. As shown in Figure 14, as the amount of the herstatin plasmid was increased relative to the HER-2 plasmid, pl85HER-2 levels were reduced and the expression of cotransfected marker GFP plasmid was reduced as in Figure 1. In contrast, as shown in Figure 13, transfection with the control GFP expression plasmid alone, which does not affect cell survival, resulted in GFP expression that was proportional to the amount of plasmid added. Therefore, reduced levels of pl85 HER-2, detected by Western analysis, appears to be related to diminished survival of the transfected cells. As shown in Figure 15, lane 6, while there was a three-fold reduction in pl85HER-2 levels, pl85 tyrosine phosphorylation signal was diminished by 25-fold, indicating an approximate 8-fold decrease in the level of tyrosine phosphorylation of pl85HER-2. The extent of inhibition of pl85 tyrosine phosphorylation was affected by the ratio of herstatin to HER-2 expression plasmid with the strongest effect observed at a ratio of 2 to 1. As sown in Figure 15, comparing lanes 1 and 6, transfection of the herstatin plasmid alone reduced the phosphotyrosine signal associated with an 185kDa protein by about two-fold. The pl85 protein comigrated with pl85HER-2 expressed from the transfected plasmid and may be endogenous pl 85HER-2. These results show that ectopic expression of herstatin reduced the tyrosine phosphorylation of exogenous pl 85HER-2, and that the ratio of herstatin to HER-2 expression plasmids affects the extent of inhibition of pl85 HER-2 tyrosine phosphorylation and the level of expression of pl85. Further these findings show that reduced pl85HER-2 tyrosine phosphorylation coπelated with diminished colony formation of cells cotransfected with HER-2 and herstatin. Herstatin expression strongly inhibits colony formation of cells that overexpress pl85HER-2. In addition, herstatin suppresses the increased survival provided by overexpression of the EGF receptor. Diminished cell survival coπespondes to a reduction in tyrosine phosphorylation of pl85HER-2 and to interference with EGF activation of the EGF receptor. Further evidence is disclosed of the inhibitory activity against pl85HER-2 and extends the negative regulatory activity of herstatin to a second member of the group I family of receptor tyrosine kinases, the EGF receptor. Herstatin inhibits pl85HER-2 tyrosine phosphorylation. Transfection of herstatin diminishes the level of constitutive tyrosine phosphorylation of ectopically expressed p 185 by 8-fold. This inhibition is observed in transfected CHO cells, HEK-293 cells and Cos-7 cells. The extent of inhibition of pl85 tyrosine phosphorylation by herstatin is dose dependent since it is affected by the amount of herstatin relative to HER-2 plasmid added to cells. Herstatin may inhibit pl 85HER-2 receptor tyrosine phosphorylation by preventing receptor dimerization, an obligatory step in receptor transphosphorylation and activation. Ectopic expression of herstatin also interfeπed with EGF-activation of the EGF receptor, a group I receptor with homology to pl85HER-2. Herstatin dampened EGF-induced receptor tyrosine phosphorylation, depressed EGF-stimulated tyrosine phosphorylation of two additional cytoplasmic proteins, and interfeπed with receptor down regulation. Receptor tyrosine phosphorylation and down regulation are halmarks of EGF activaton of the EGF receptor. The inhibition of EGF receptor by herstatin occuπed at saturating concentrations of EGF. The intron 8-encoded domain at the C-terminus of herstatin, which appears to confer high affinity binding to pl85HER-2, binds to the EGF receptor but does not compete with EGF binding (Doherty et al., submitted). These results suggest that herstatin blocks activation of the EGF receptor by a mechanism that does not involve competition with growth factor binding. Other studies show that herstatin associates with the EGF receptor in "pull-down" assays (Doherty, submitted). Since herstatin appears to form a stable complex with the EGF receptor, it may inhibit EGF-induced homodimers. It is also possible that herstatin inhibits the EGF receptor indirectly by preventing EGF-induced heterodimer formation or by an alternative mechanism that does not involve complex formation with the receptors. Overexpression of pl85HER-2 or the EGF receptor enhances cell growth and/or survival. Transfection of cells with pl 85HER-2 enhanced the number of colonies surviving G418 selection by about 60%> and transfection with the EGF receptor increased the number of cell colonies by about 40% compared to the control transfected cells. Herstatin, transfected alone, did not significantly alter the number of colonies, although it did appear to decrease the tyrosine phosphorylation associated with an endogenous protein that comigrated with pl85HER-2 in CHO cells. This may have been pl85HER-2, since the CHO cells have little or no group I receptors other than pl85HER-2. Herstatin co-expressed with the EGF receptor suppressed colony formation relative to cells transfected with the EGF receptor alone. Since herstatin also inhibited EGF activation of the EGF receptor, and has been found to form stable complexes with the EGF receptor (Doherty, submitted), the growth inhibition may have been due to herstatin forming an inactive kinase complex with the EGF receptor or through inhibition of transactivation of the EGF receptor by forming a complex with endogenous p 185HER-2. It is also possible that the growth inhibition may have been caused by a separate unknown activity of herstatin that does not directly involve interaction with the EGF receptor or pl 85HER-2. The most potent growth inhibitory activity was observed when herstatin was co-expressed with pl 85HER-2. With this combination, there was a 7-fold reduction in colony formation relative to cells transfected with pl85HER-2 alone, and a 5-fold inhibition compared to cells transfected with herstatin alone or compared to confrol transfected cells. Overexpressed pl85HER-2 is constitutively active in cells, and blocking its activity may inhibit cell survival as well as growth. In comparison to other group I RTK inhibitors that have been described, herstatin has several similarities with the pl85neu ectodomain mutant, which functions as a dominant-negative inhibitor. Like herstatin, the membrane-anchored dominant-negative neu mutant contains sequences from the receptor extracellular domain and forms complexes with RTKs (Doherty et al., and Greene). Also, both pl85 neu ectodomain and ectopically expressed herstatin are capable of inhibition of pl85HER-2 as well as the EGF receptor. In contrast to the previously described pl 85neu ectodomain, which consists of subdomains I through IV and a membrane anchor, herstatin contains only subdomains I and II from the pl85 ectodomain, and is a naturally occuring product expressed in fetal kidney and fetal liver cells (Doherty et al.,1999). Herstatin is secreted and does not require a membrane anchor to form complexes nor to exert its inhibitory activity. Example 1 This example provides the results from an experiment to investigate HER-2 mRNA diversity within the extracellular domain (ECD) coding sequence using polymerase chain reaction (PCR). A cDNA library from SKOV-3 cells (American Type Culture Collection (Rockville, MD) maintained in DMEM, supplemented with 10%> fetal bovine serum and 0.05%> gentamycin), an ovarian carcinoma cell line in which the HER- 2 gene is amplified eight times (Tyson et al., Am. J. Obstet. Gynecol. 165:640-646, 1991) was examined using a forward primer specific for exon 1 (Tal et al., Mol. Cell. Biol. 1, 2597-2601, 1987) identical to nucleotides 142-161 and a reverse primer complementary to nucleotides 1265-1286 in exon 9 (Scott et al, Mol Cell. Biol. 13:2247-2257, 1993). Briefly, The SKOV-3 cDNA library was provided by Origene Technologies, Inc. (Rockville, MD), and was prepared from RNA extracted from SKOV-3 cells. RNA was extracted from SKOV-3 cells grown to 80%> confluence on 15 cm plates with TriReagent (Molecular Research Center, Inc., Cincinnati, OH), according to the manufacturer's protocol, to obtain total RNA. RNA was resuspended in lOmM Tris-EDTA, pH 8.0, for reverse franscription and cDNA library construction, or in RNA hybridization buffer (80% formamide, 40mM PIPES, 4 mM NaCl, ImM EDTA, pH 7.5) for ribonuclease protection assay (RPA). RNA concentrations were determined spectrophotometricaUy at OD 260 . Poly A + mRNA was selected from total RNA using a mRNA extraction kit (Oligotex, Qiagen). A product of -1420 bp, determined to be HER-2-specific by Southern blotting, was approximately 270 bp larger than the expected size of 1144 bp from the previously described cDNA sequence (Coussens et al., Science 230:1132-1139, 1985). Briefly, the Southern blotting procedure transfeπed nucleic acids from agarose gels under vacuum (Bio-Rad Model 785 Vacuum Blotter) in 0.4 M NaOH to Gene Screen Plus Hybridization Transfer Membrane (NEN Research Products, Boston, MA). Nucleic acids were fixed to membranes by UV crosslinking in a UV-Sfratalinker (Stratagene, Inc., La Jolla, CA), and the membranes were blocked in hybridization buffer (50%) formamide, 5X SSC, 1% SDS, 10 mg/ml herring sperm DNA) at 42 °C for 2 h. The membranes were hybridized at 42 °C for 16 h in hybridization buffer with 10 7 cpm of a 220 bp Kpn-HincII fragment from ECDIIIa cDNA labelled with (α- 32 P)dCTP (NEN Life Sciences) using a Random Prime DNA Labelling Kit (Boehringer Mannheim). Templates were amplified in a Perkin Elmer GeneAmp PCR System 2400 (Perkin Elmer Cetus, Emeryville, CA) using the Expand High Fidelity PCR System (Boerhinger Mannheim) with IX High Fidelity PCR buffer containing 2.5 mM MgCl 2 , 5 μM of each primer, and 200 μM dNTPs. All primers were obtained from GIBCO BRL (Life Technologies). Numbering of nucleotide and amino acid residues is according to the HER-2 cDNA sequence reported by Coussens et al. (Coussens et al., Science 230:1132-1139, 1985). The HER-2 extracellular domain was targeted for amplification from an SKOV-3 cDNA library (Origene Technologies, Inc.) using a forward primer (A) identical to nucleotides (nt) 142- 161 of HER-2 cDNA (5 ' - TGAGCACCATGGAGCTGGC-3' [SEQ ID NO 3]), which spans the initiation codon (underlined) and a reverse primer (B) (5'-TCCGGCAGAAATGCCAGGCTCC-3' [SEQ ID NO 4]), which is complementary to HER-2 exon sequence at nt 1265-1286. Cycling parameters were: 94 °C, 30 sec; 58 °C, 45 sec; 68 °C, 3 min, for 30 cycles. The region spanning the alternative sequence (denoted ECDIIIa) from genomic DNA, was amplified using a forward primer (C) (5'-AACACAGCGGTGTGAGAAGTGC-3' [SEQ ID NO 5]) identical to HER-2 exon-specific sequence at nt 1131-1152 and the reverse primer (B) [SEQ TD NO. 4] on DNA prepared as described (Bond et al, FEBS Letters 367:61- 66, 1995) with cycling parameters: 94 °C, 30 sec; 62 °C, 30 sec; 72 °C, 60 sec, for 25 cycles. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to investigate the structure of mRNA containing the ECDIIIa sequence. First strand cDNA was reverse transcribed (Bond et al., FEBS Letters 367:61-66, 1995) using 5 μg RNA primed with 0.5 μg oligo-dT. To amplify the ECDIIIa insert and adjacent 5' HER-2 exon sequence, a forward primer (A) described above and a reverse primer (D) (5'- ATACCGGGACAGGTCAACAGC-3' [SEQ ID NO 6]) which is complementary to the 3'ECDIIIa-specific sequence were used. Cycling parameters were: 94 °C, 30 sec; 60 °C, 40 sec; 68 °C, 2 min, for 30 cycles. Amplification of the ECDIIIa insert and adjacent 3' HER-2 exon-specific sequence was with a forward primer (E) (5 '-TCTGGGTACCCACTCACTGC-3 ' [SEQ ID NO 7]) which is identical to the 5'ECDIIIa-specific sequence and contains a Kpnl restriction site and a reverse primer (F) (5'-TTCACACTGGCACGTCCAGACC-3' [SEQ ID NO 8]) which is complementary to HER-2 exon sequence at nt 3898-3919 and spans the termination codon (underlined). Cycling parameters were: 94 °C, 30 sec; 60 °C, 40 sec; 68 °C, 5 min, for 30 cycles. The PCR product was subcloned and the nucleotide sequence was determined. The results showed that the normal HER-2 coding sequence was present beginning with the 5' primer sequence and continued uninterrupted through nucleotide 1171. At this position, a 274 nucleotide insertion was found, followed by the expected coding sequence, including the 3' primer sequence. Analysis of the predicted protein product showed that the 274 nucleotide insertion encodes an extension of the known HER-2 protein, beginning at residue 340 (Coussens et al., Science 230:1132-1139, 1985), and introduces an in-frame stop codon 79 amino acids later (Figure 1). Comparison of the inserted nucleotides and their predicted amino acid sequence with sequences in Genbank showed no homologies. Examination of the 5' and 3' junctions of the divergent sequence revealed consensus splice donor and acceptor sites (Sharp, and Burge, Cell 91 : 875-879, 1997) and include a pyrimidine tract and potential branchpoint adenine residues near the 3 'end of the insert sequence (Figure 1). Thus, the inserted sequence is likely to be an intron. Inspection of the predicted amino acid sequence of the novel 79 amino acids [SEQ ID NO. 1] encoded by the inserted sequence shows a consensus N-linked glycosylation site and a high proline content of 19% (Figure 1). The inserted sequence was designated ECDIIIa since it is located at the boundary between subdomains II and III in the extracellular domain of the pl85HER-2 sequence (Lax et al., Mol. Cell. Biol. 8:1831-1834, 1988). The insert sequence is in-frame with the adjacent 5' HER-2 exon sequence for 236 nt where there is a termination codon. Example 2 This example provides the results from experiments characterizing ECDffia as contiguous with HER-2 exons in the genome. To investigate the HER-2 gene structure in the region of the ECDIIIa sequence, a forward primer, identical to nucleotides 763- 785, and a reverse primer, complementary to nucleotides 1265-1286 of the HER-2 cDNA, were used in the PCR on human genomic DNA. The amplification product was anticipated to span exon 5 (Tal et al., Mol. Cell. Biol. 7:2597-2601, 1987) to an exon which is immediately 3' of the ECDIIIa sequence. Intron number and sizes were estimated based on PCR product sizes, restriction digest analysis, and partial sequence analysis of amplification products. Next, human genomic DNA was examined using HER-2 exon-specific primers that directly flank the insert to determine the sequences immediately flanking the ECDIIIa sequence. A ~430 bp product was amplified from normal human genomic DNA and from genomic DNA extracted from carcinoma cell lines SKOV-3, SKBR-3 and BT474, all of which have HER-2 gene amplification (Kraus et al., EMBO J. 6:605- 610, 1987) and were found to express ECDIIIa in their cDNA. The identities of the PCR products as HER-2 were verified by Southern blot analysis using the procedure described in Example 1. Nucleotide sequence analysis showed that the PCR product from human genomic DNA contained the ECDIIIa insert, flanked immediately on both sides by known HER-2 coding sequence; no mutations or reaπangements were seen. These data show that the ECDIIIa sequence represents a wholly retained intron, likely intron 8 based on the size of products amplified following intron 4 and on the location of intron 8 in the homologous EGFR gene and HER-3 gene (Lee and Maihle, Oncogene 16:3243-3252, 1998). Example 3 This example shows that ECDIIIa is the only retained intron within the coding sequence of HER-2 mRNA. To determine whether additional introns were retained in the mRNA containing the ECDIIIa insert sequence, the reverse transcriptase-polymerase chain reaction (RT-PCR) was employed. First, a forward primer identical to 5' HER-2 cDNA sequence at 142-161 which spans the initiation codon, and a reverse primer complementary to the 3' ECDIIIa sequence were employed with SKBR-3 and SKOV-3 cDNA. A product of 1.3 kb was amplified, which is the size expected if the product contained no introns other than intron 8. Amplification of the 3 'HER-2 coding sequence was then performed using a forward primer identical to 5' ECDIIIa sequence and a reverse primer complementary to 3ΗER-2 cDNA sequence at nucleotides 3898-3919, which spans the pl85HER-2 termination codon. A product of 2.9 kb was amplified, which is the size expected from the HER-2 cDNA if no additional introns were retained. Further characterizations of both the 5 '(1.3 kb) and 3 '(2.9 kb) amplification products by restriction digest analysis and nucleotide sequencing confirmed the absence of additional retained introns. To determine the size of the products amplified when intron sequences are included, genomic DNA was used as a template for the PCR reactions, which resulted in products of approximately 10 kb for the 5' coding sequence and 5 kb for the 3' coding sequence. These results indicate that the alternative HER-2 transcript, resulting from retention of an intron of 274 bp, was expected to be about 4.8 kb in size, assuming that the 5 'untranslated (5'UTR) and 3 'untranslated (3'UTR) regions are identical in size to the previously described ~4.5 kb HER-2 cDNA (Coussens et al., Science 230:1132-1139, 1985). Example 4 This example illusfrates the expression of a protein containing an ECDIIIa sequence. To assess whether the alternative sequence is translated into a protein product, the ECDIIIa sequence was expressed as a polyhistidine-tagged peptide in bacteria, purified the peptide by nickel-affinity chromatography, and raised antisera against the purified peptide. Briefly, the bacterial expression vector was prepared by amplifying the ECDIIIa sequence from the SKOV-3 cDNA library using primer E and a reverse primer complementary to the 3' end of the ECDIIIa insert sequence. The reverse primer contained a BamHl restriction site sequence, and was identical to that used for template construction in the RPA (described in examples land 2). The PCR amplification product of ~280 bp was digested with Kpnl and BamHl, gel purified (Qiaex II, Qiagen, Chatsworfh, CA), and cloned into the pET30a vector, which encodes a six histidine tag at the amino-terminus of the expressed protein (Novagen, Madison, WI). The resulting expression vector, pET-ECDIIIa, was used for transformation of bacterial strain BL21. To express the ECDIIIa protein product, BL21 cells transformed with the pET- ECDIIIa expression vector were grown in LB broth with 30 μg/ml Kanamycin for 4 h at 37 °C. Expression was induced with 0.1 mM IPTG for 3 h and the harvested cells were lysed by sonication, and then centrifuged at 39,000 x g for 20 min. The supernatant was absorbed onto Ni-NTA agarose (Qiagen), by shaking for 60 min at room temperature. The resin was washed with ten volumes of wash buffer (10 mM Tris pH 7.9 and 300 mM NaCl), followed by ten volumes of wash buffer with 50 mM imidazole. The his-tagged ECDIIIa protein was eluted in wash buffer with 250 mM imidazole. The his-tagged protein, which was estimated to be approximately 90% pure by Coomassie Blue staining of gels, was used to generate and characterize antibodies. Briefly, anti-ECDIIIa antisera were produced by Cocalico Biologicals, Inc. (Reamstown, PA) by injection of two rabbits with purified polyhistidine-tagged ECDIIIa peptide (described below). Polyclonal anti-neu (N) was produced against a peptide identical to amino acid residues 151-165 of pl85HER-2 (Lin and Clinton, Oncogene 6:639-643, 1991). Polyclonal anti-neu (C) was made against a peptide identical to the last 15 residues of the carboxy-terminus of pl85HER-2 (Lin et al., MolCell Endocrin. 69: 111-119, 1990). Antisera from two immunized rabbits were characterized and found to contain antibodies of high titer that reacted with the purified ECDIIIa peptide. A Western blot analysis examined whether SKBR-3 cells, which expressed the alternative sequence in its cDNA, produced a protein that reacts with anti-ECDIIIa antibody. A 68 kDa protein from the cell extract and from the extracellular media reacted with anti-ECDIIIa antibody from two different rabbits diluted at least 20,000 fold, but not with preimmune sera. Inspection of the cDNA sequence of the alternative transcript (Figure 1) predicted a secreted protein product of 65-70 kDa if all 5 consensus N-linked glycosylation sites in the N-terminal pl85HER-2 sequence were glycosylated (Stem et al., Mol. Cell. Biol. 6:1729-1740, 1986). If the 68 kDa ECDlTJa protein [SEQ ID NO. 2] is the translation product of the alternative HER-2 mRNA, then its N-terminal residues should be identical to the N- terminal 340 residues of pl85HER-2. Therefore, cell extract from SKBR-3 cells was immunoprecipitated with anti -peptide antibody against an N-terminal sequence of HER- 2, anti-neu (N) (Lin and Clinton, Oncogene 6:639-643, 1991) or with anti-ECDIIIa, and the immune complexes were examined by Western blot analysis with both antibodies. Briefly, three to 5 μl of antisera were added to 2 mg of protein from cell lysates prepared in M-RIPA buffer (1% Nonidet P-40, 50 mM Tris pH 7.4, 0.1% sodium deoxycholate, 150 mM NaCl, 1 mM PMSF, 1%> aprotinin), which had been centrifuged to remove nuclei. Immunoprecipitation was for 2 h with shaking at 4 °C as described (Lin et al., MolCell Endocrin. 69:111-119, 1990). The immune complexes were bound to Protein G Sepharose (Pharmacia) by incubation for 1 h at 4 °C with shaking, collected by centrifugation, and washed four times with M-RIPA. The proteins were released from the immune complex by incubation at 95° C for 2 min in SDS-PAGE sample buffer and resolved by SDS-PAGE in 7.5% gels (Mini-Protean II electrophoresis cell, Bio-Rad). Western blotting was conducted following SDS-PAGE. Proteins were elecfroblotted onto nitrocellulose (Trans-blot, BioRad) using a semi-dry transfer unit (Bio-Rad) at 15 V for 20 min per gel (0.75 mm thick) equilibrated with 25 mM Tris pH 8.3, 192 mM glycine, 50 mM NaCl, and 20%> methanol. The membranes were blocked with 5% nonfat dry milk at 25 °C for one hour. The blots were then incubated with primary antibody, washed twice for 15 min, and four times for 5 min with TBS-Tween (Tris-buffered saline containing 0.05%> Tween), and then incubated for 40 min with goat anti-rabbit secondary antibody, conjugated to horseradish peroxidase (Bio-Rad), diluted 1:10, 000 in TBS-Tween. After incubation with secondary antibody, the membranes were washed as described above and reacted with chemiluminescent reagent (Pierce) and then were exposed to Kodak X-OMAT BLU film. As expected, p68HER-2 was detected when anti-ECDIIIa was used for immunoprecipitation and for Western blot analysis. When anti-ECDIIIa was used for immunoprecipitation and anti-neu (N) was the probe in the Western blot, a 68kDa protein was detected, indicating that p68ECDIIIa contained the N-terminal sequence of pl85HER-2. Further, anti-neu (N) precipitated p68HER-2, which was detected by probing with anti-ECDIIIa antibody. These results demonstrate that p68HER-2 contains both ECDIIIa and the N-terminal sequence of HER-2. Several other cell lines were examined for expression of p68ECDIIIa. The carcinoma cell lines which contained ECDIIIa sequence in their cDNA (BT474, SKOV- 3) also had p68HER-2. Of several cell lines examined, HEK293 cells, derived from normal human embryonic kidney cells, expressed the highest levels of p68ECDIIIa in the cell extract and in the extracellular media, at about 5 to 10-fold higher amounts than SKBR-3 cells. In comparison to the carcinoma cell lines examined (SKBR-3, SKOV-3, and BT474) which overexpress pl85HER-2, the HEK293 cells contained about 20 fold lower amounts of pl85HER-2. Therefore, the relative proportion of p68HER-2 to pl85HER-2 was at least 100 fold greater in HEK293 cells than in the three carcinoma cell lines studied. Reactivity with p68HER-2 as well as with a protein of -120 kDa, particularly apparent in the HEK293 extracts, was blocked by preincubation of the antisera with purified ECDIIIa peptide demonstrating sequence-specific reactivity. The larger protein may be a dimer of p68HER-2. Therefore, p68HER-2 was expressed and secreted from several carcinoma cell lines and is at 5-10 fold elevated levels in HEK293. Example 5 This example illusfrates expression of an alternative HER-2 transcript containing the ECDIIIa intron sequence. Results of the RT-PCR analysis indicated that the ECDIIIa sequence was inserted into an otherwise normal-sized HER-2 mRNA. These data suggest an alternative transcript of -4.8 kb. To examine the size and expression of the ECDIIIa alternative transcript, Northern blot analysis was conducted using an ECDIIIa- specific probe. Briefly, a template for antisense RNA probe synthesis was constructed from SKOV-3 cDNA by PCR amplification of a 389 bp sequence spanning the entire ECDIIIa insert sequence and containing adjacent 5 'HER-2 exon sequence. The PCR was done using the forward primer C [SEQ ID NO. 5] that is identical to HER-2 cDNA sequence at nt 1131-1152 and a reverse primer (5 ' - GCACGGATCCATAGCAGACTGAG GAGG-3' [SEQ ID NO. 9]) which contains a 3' E mHl restriction endonuclease site and is complementary to the sequence spanning the 3' splice site of the ECDIIIa sequence. The PCR product was then digested with BamRl, liberating a 375 bp fragment, which was cloned into pBluescript SK (Stratagene). The plasmid was sequenced by the Vollum Institute Core Sequencing Facility (Portland, OR) with ml 3 forward and reverse primers. An antisense RNA probe complimentary to the entire ECDIIIa sequence and to 87 nt of HER-2 exon sequence 5' to the insert was transcribed from 1 μg of linearized template using (α- 32 P) CTP, T7 RNA polymerase, and the T7/SP6 Riboprobe Synthesis System (Promega, Madison, WT). This probe was expected to protect a 370 nt fragment when hybridized with mRNA containing ECDIIIa and adjacent HER-2 exon sequence, and to protect an 87 nt fragment when hybridized with fully spliced HER-2 mRNA. To prepare the RNA hybrids, 30 μg of RNA were hybridized with approximately 50,000 cpm of antisense RNA probe at 48 °C for 16 h. RNA hybrids were digested for 30 min at 37 °C with 40 μg/ml RNaseA (Boerhinger Mannheim) and 2 μg/ml RNase Tl (Life Technologies) in a solution of 250 mM NaCl, 5 mM EDTA, and 10 mM Tris pH 7.5. Proteinase K (100 μg) (Life Technologies) in 20 μl 10% SDS was added to stop the digestion. Samples were extracted with acid phenol (pH 4.5; Life Technologies) and chloroform, precipitated with two volumes of 100%> ethanol, and suspended in 5 μl of RPA sample buffer (88% formamide, 10 mM EDTA pH 8.0, 1 mg/ml xylene cyanol, and 1 mg/ml bromophenol blue). Samples were denatured at 95° C for 10 min and elecfrophoresed on a 5%> polyacrylamide/urea gel in TBE (89 mM Tris, 89 mM borate, 2 mM EDTA pH 8.3). Gels were dried under vacuum and subjected to phosphorimager analysis for quantitation of the protected fragments (IP Lab Gel, Molecular Dynamics). An alternative transcript of approximately 4.8 kb was detected in HEK293 cells which expressed the highest levels of p68ECDIIIa. However an alternative transcript could not be detected by Northern analysis of the SKBR-3, BT474, or SKOV-3 carcinoma cell lines. Therefore, the more sensitive ribonuclease protection assay (RPA) was employed to examine the expression levels of the alternative transcript relative to the fully spliced 4.5 kb transcript. RNA from ovarian (SKOV-3) and breast (SKBR-3 and BT474) carcinoma cell lines, which contained detectable levels of p68ECDIIIa, and a control cell line, 17-3-1, stably transfected with HER-2 cDNA, were hybridized with an antisense 32 P-labeled RNA probe which spanned the entire ECDIIIa (intron 8) sequence and 5' HER-2 exon sequence flanking intron 8. Following RNase digestion, electrophoresis, and autoradiography, a band of 370 nucleotides was detected in each cell line except for 17-3-1, which coπesponds to the expected size protected by an ECDIIIa- containing HER-2 mRNA. In addition, an 87 nucleotide protected fragment was detected in all cells and is the size expected for the fully-spliced HER-2 message which is overexpressed by more than 100 fold in these carcinoma cell lines compared to normal control cell lines (Kraus et al., EMBO J. 6:605-610, 1987). The amounts of each protected fragment were quantitated and normalized for size to estimate the relative abundance of the alternative transcript, expressed as a percentage of the pl85HER-2 mRNA. The alternative HER-2 mRNA with the ECDIIIa insert was at 4.2% the level of the fully spliced transcript in SKOV-3; 5.4% in SKBR-3, and 0.8% in BT474 cells. Example 6 This example shows that alternative transcripts containing the ECDIIIa insert were expressed in human embryonic kidney and liver. A Northern blot was conducted to examine whether an alternative transcript, which contains the ECDIIIa sequence, was expressed in normal human tissue. PolyA + mRNA from a variety of human fetal tissues prepared as a Northern blot was hybridized with a radiolabeled probe specific for the unique ECDIIIa sequence. A 4.8 kb mRNA was detected in kidney and a 2.6 kb transcript was detected in liver (Figure 2). The 4.8 kb transcript likely coπesponded to the full length 4.5 kb transcript with the 274bp insert and the 2.6 kb transcript may have coπesponded to a previously described 2.3 kb alternative transcript (Yamamoto et al., Nature 319:230-234, 1986; and Scott et al., Mol. Cell. Biol. 13:2247-2257, 1993) with the 274bp ECDIIIa insert. When the blot was stripped and hybridized with a probe specific for the 5' HER-2 coding sequence, a broad band representing the 4.8 and 4.5 kb mRNAs was detected in fetal kidney tissues and the truncated 2.6 kb transcript was detected in liver showing that these alternative transcripts contain sequences that encode the HER-2 ECD. Because the inserted ECDIIIa sequence contained a termination codon, the same protein product may be produced from each of these mRNAs. Several cell lines were also investigated for the ECDIIIa-containing alternative transcript by Northern blot analysis. The 4.8 kb alternative transcript was detected in the human embryonic kidney cell line, HEK-293 (Figure 2). Although the ECDIIIa sequence was detected by RT-PCR analysis of SKBR-3, BT474, and SKOV-3 carcinoma cell lines, which all contain HER-2 gene amplification, an ECDIIIa-containing alternative transcript could not be detected by Northern analysis of these cells. Therefore, the more sensitive ribonuclease protection assay (RPA) was employed using an antisense probe which spanned the entire ECDIIIa sequence and 5' HER-2 exon sequence flanking the ECDIIIa sequence. The alternative HER-2 mRNA with the ECDIIIa insert was detected at less than 5%> of the fully spliced transcript in SKOV-3, SKBR-3, and BT474 cells. These findings show that two alternative transcripts containing the ECDIIIa sequence were expressed in a tissue-specific manner in normal human tissues, that the 4.8 kb alternative transcript was expressed in the HEK-293 cell line, and that the carcinoma cells with gene amplification express reduced amounts of the alternative transcript at less than 5% of the 4.5 kb HER-2 transcript. Example 7 This example illusfrates expression of a protein containing the ECDIIIa sequence. To assess whether the alternative sequence was translated into a protein product, the ECDIIIa sequence, as a polyhistidine-tagged peptide in bacteria, was expressed and purified by nickel-affinity chromatography, and raised antisera against the purified peptide. The HEK-293 cells, which expressed the 4.8 kb ECDIIIa alternative transcript, were examined for expression of an ECDIIIa-containing protein by Western analysis. A 68 kDa protein from the cell extract and from the extracellular media reacted with the anti-ECDIIIa antibody (Figure 3) but not with preimmune sera and reactivity was blocked by preincubation of the antisera with purified ECDILTa peptide (Figure 3). The larger protein of -125 kDa detected in some cell extracts may be an aggregate of p68HER-2. The cDNA sequence of the alternative transcript (Figure 1) predicts a secreted protein product of 65-70 kDa if all 5 consensus N-linked glycosylation sites in the N-terminal pl85HER-2 sequence are glycosylated (Stem et al., Mol. Cell. Biol. 6: 1729-1740, 1986). Several other cell lines were examined for expression of p68ECDIIIa. The carcinoma cell lines which contained ECDIIIa sequence in their cDNA (BT474, SKOV-3, SKBR-3) also had detectable levels of p68HER-2. Example 8 This example illustrates the expression of p68HER-2 relative to pl85HER-2 was markedly reduced in carcinoma cell lines in which the HER-2 gene is amplified. Because the p68HER-2 mRNA was expressed at very low levels relative to the pl 85HER-2 mRNA in carcinoma cell lines with HER-2 gene amplification, the relative proportions of p68HER-2 and pl85HER-2 proteins in several cell lines were examined with and without HER-2 gene amplification. Western blots were prepared and probed with both antisera specific for p68HER-2 and for pl85HER-2. Figure 4 shows that pl85HER-2 was readily detected in the carcinoma cells lines that have their HER-2 gene amplified about 8 times (Kraus et al, EMBOJ. 6:605-610, 1987). However, there was not a coπesponding elevation in p68HER-2. In comparison, p68HER-2 was the only HER-2 protein detected in the HEK-293, IOSEVAN, and HBL100 nontumorigenic cells, although pl85HER-2 was expressed at very low levels in these cells (Kraus et al., EMBO J. 6:605-610, 1987) and was detected in overexposed blots. These data show that p68HER-2 was low in proportion to pl85HER-2 in carcinoma cells with HER-2 gene amplification and suggests that a mechanism may exist to maintain low levels of p68HER-2 when pl85HER-2 is overexpressed. Example 9 This example illustrates that p68HER-2 and the ECDIIIa peptide specifically bind to pl85HER-2. Because p68HER-2 is secreted and contains subdomains I and II identical to l85HER-2, in addition to a novel sequence, the possibility that p68HER-2 may interact with pl85HER-2 was investigated. Antipeptide antibody against the N- terminus of pl85HER-2 and p68HER-2, anti-neu (N), or antibody specific for pl85HER- 2, anti-neu(C), were used for immunoprecipitations of SKBR-3 carcinoma cells, which express low levels of p68HER-2 and overexpress pl85HER-2. The immunoprecipitated material was prepared as a Western blot and probed with both anti-ECDIIIa specific for p68HER-2 and with anti-neu(C). Anti-neu (N) immunoprecipitated both p68HER-2 and l85HER-2 (Figure 5 A). In addition, antibodies specific for the C-terminus of pl85HER-2 immunoprecipitated pl85HER-2 and coprecipitated p68HER-2 (Figure 5 A), suggesting an interaction between the two proteins. Since binding interactions between ECD sequences are very weak (Tzahar et al., EMBOJ. 16:4938-4950, 1997; Fitzpatrick et al., FEBS Letters 431:102-106, 1998), the possibility that binding may be confeπed by the novel prolme rich ECDIIIa domain was examined. The unique 79 amino acid domain, purified as a His-tagged protein, was immobilized on nickel agarose and used in a pull-down assay. For controls, two purified His- tagged peptides unrelated to ECDIIIa, a 600 residue fragment of the Wilson's disease membrane protein, and a 70 residue fragment containing the DNA binding domain of the CREB protein, were likewise immobilized on nickel agarose resin. The immobilized peptides were incubated with protein extracts prepared from HER-2 transfected 3T3 cells (17-3-1). Following extensive washes, the bound proteins were eluted and prepared as a Western blot which was probed with an antibody specific for pl85HER-2. Equal amounts of His-tagged ECDIIIa peptide and control peptide were bound to the resin as confirmed by elution with IM imidazole and Coomassie staining of the eluted material in SDS-gels. While no pl85HER-2 was retained by resin without peptide or with control peptide, pl85HER-2 was selectively retained by the ECDIIIa peptide (Figure 5B). Since the ECDIIIa domain bound to pl85HER-2 in a pulldown assay, the question of whether the ECDIIIa domain preferentially binds to cells that overexpress pl85HER-2 was examined. This was investigated using monolayer cultures of 17-3-1 cells transfected with HER-2 compared to the parental 3T3 cells. The cells were incubated with different concentrations of the His-ECDIIIa peptide, washed, and extracted in denaturing buffer with protease inhibitors. To detect any bound peptide, the cell extracts were examined by Western blot analysis using antibodies specific for ECDIIIa. In addition, equal aliquots of the ECDIIIa peptide treated cells were reacted as a Western blot with antibodies specific for pl85HER-2, demonstrating the overexpression of pl85HER-2 in the transfected 17-3-1 cells. The ECDIIIa peptide preferentially bound to intact 17-3-1 cells at nM concentrations (Figure 5C) whereas little or no peptide was found to bind to equivalent amounts of parental 3T3 cells suggesting a specific interaction with the extracellular domain of pl85HER-2. Example 10 Effect of p68ECDIIIa and the ECDIIIa peptide on tyrosine phosphorylation of pl85HER-2 was examined. Tyrosine phosphorylation of RTKs is the initial indication of ligand activation and signal transduction. Tyrosine phosphorylation in 17-3-1 cells treated with different amounts of the purified ECDIIIa peptide, with conditioned media (CM) from HEK293 cells that contained high levels of p68HER-2 (Figure 2A), or with control, conditioned media from SKOV-3 cells that had no detectable p68HER-2 were examined. There was no increase in the tyrosine phosphorylation signal at 10 minutes (Figure 6) or 2 hrs of treatment with His-ECDIIIa or with concentrated CM suggesting that pl85HER-2 was not activated. Neither p68HER-2-containing CM nor the ECDIIIa peptide detectably altered the phosphotyrosine signal coπesponding to pl85HER-2 from SKOV-3 cells in which p 185HER-2 tyrosine phosphorylation levels were low. Additionally, p68HER-2 and the ECDIIIa peptide had no discemable effect on in vitro self-phosphorylation activity of pl85HER-2 immunoprecipitated from 17-3-1 cell extracts. These results support the conclusion that p68HER-2 did not activate pl85HER- 2 signal transduction. Example 11 This example illusfrates that the sequence of intron 8 is polymorphic. Intron 8 of the human HER-2 gene is alternatively retained in mRNA, and encodes a novel 79- residue domain at the C-terminus of a part of the extracellular domain of pl85HER-2. The product, "herstatin," of the alternative transcript with the retained intron functions as an autoinhibitor of the HER-2 oncogene. The intron 8 encoded domain, alone, was shown to bind with nM affinity to pl85HER-2. (Doherty et al., Proc. Natl. Acad. Sci. USA 96:10,869-10,874, 1999). Additionally, polymorphisms in the nucleotide and deduced amino acid sequence of intron 8 in the HER-2 gene were identified by sequencing genomic DNA from 15 different individuals. Figure 8 and SEQ ID NO: 1 show the most common nucleotide and coπesponding amino acid sequences, respectively, of intron 8. This region contains 10 different polymorphisms (marked by the letters W (2x), Y (3x), R, N, M, and S (2x) in SEQ ID NO:10; or marked by an "X" in Figure 8) that result in nonconservative amino acid substitutions (see legend to TABLE 1). For example, the polymorphism (G — » C) at nucleotide position 161 (Figure 8; TABLE 1) would result in a substitution of Arginine (R) for Proline (P) at amino acid residue #54 of SEQ ID NO:l, or residue #394 of SEQ ID NO:2.. The N-terminal Glycine (G), designated as position 1 in Figure 8 or SEQ ID NO: 10, coπesponds to amino acid residue #341 in the "herstatin" sequence (Doherty et al., Proc. Natl. Acad. Sci. USA 96:10,869-10,874, 1999). The nucleotide sequence shown in Figure 1(A) is a polymorphic form that differs at amino acid residues #6 and #73 from the most commonly detected sequence shown here in Figure 8. This result demonstrates that in the human population there are several variations in the intron-8 encoded domain that could lead to altered biochemical and biological properties among ECDIIIa-containing protein variants. Some identified variants are summarized in Table 1 : TABLE 1 Table 1. Sequence variants in the intron-8 encoded domain found in the human population (based on 15 different individuals). Sequence variants 1-11 are listed, showing the base changes at a particular "X" positions relative to that found in the most common DNA sequence shown in Figure 8. The numbers in parenthesis after each X coπespond to the nucleotide position in the DNA sequence shown in Figure 8. The DNA sequence variants listed here coπespond to the variable amino acid positions ("Xaa") of SEQ ID NO:l as follows: X(4) to Xaa(2); X(14) to Xaa(5); X(17) to Xaa(6); X(47) to Xaa(16); X(54) to Xaa(18); X(62) to Xaa(21); X(106) to Xaa(36); X(161) to Xaa(54); X(191) to Xaa(64); X(217) to Xaa(73); and to the variable amino acid positions of SEQ ID NO:2 as follows: X(4) toXaa(342); X(14) to Xaa(345); X(17) to Xaa(346); X(47) to Xaa(356); X(54) to Xaa(358); X(62) to Xaa(361); X(106) to Xaa(376); X(161) to Xaa(394); X(191) to Xaa(404); X(217) to Xaa(413). The specific amino acid changes (relative to the most common DNA sequence of Figure 8) for the variable amino acid positions in SEQ ID NO:l are: Variant 1, Xaa(2)(Thr→Ser); Variant 2, Xaa(5) (Leu-»Pro); Variant 3, Xaa(6) (Pro→Leu); Variant 4, Xaa(16) (Leu- Gln); Variant 5, Xaa(l 8) (Met→Leu); Variant 6, Xaa(21) (Gly→Asp, Alu or Val); Variant 7, Xaa(36) (Leu→Ile); Variant 8, Xaa(54) (Pro→Arg); Variant 9, Xaa(64) (Pro→Leu); Variant 10, Xaa(73) (Asp→Asn), and Variant 11, Xaa(6) (Pro→Leu) and Xaa(73) (Asp→Asn). The same substitutions apply to the coπesponding variable amino acid positions in SEQ ID NO:2. Example 12 Co-expression of Herstatin in Transfected Cells (1) Inhibited pl85HER-2 Levels by Inhibiting Colony Formation, and (2) Inhibited pl85HER-2 Tyrosine Phosphorylation The effects of the herstatin p 185HER-2 binding interaction were further characterized by co-fransfecting Cos-7 cells with herstatin and pl85HER-2-expressing vectors, and measuring the resulting l 85HER-2 levels, and the extent of post- transfection colony formation and pl85HER-2 tyrosine phosphorylation. Methods. Transfection of Cos-7 cells. Cos-7 cells were plated at 2 xlO 5 cell/well into 6-well plates and transfected using Lipofectamine (BioRrad). The cells were transfected with 1.5 μg of herstatin expression vector plus 1.5 μg of HER-2 expression vector, or with 1.5 μg of HER-2 expression vector (all pcDNA3.1 from Invitrogen). The total amount of DNA in each case was adjusted to 3 μg with control empty vector. The transfected cells were analyzed after 48 hrs by Western blot analysis, and protein was detected with antibodies against pl85HER-2, anti-neu (C), or with antibodies against the intron 8-encoded C-terminal sequence of herstatin (anti-Hst). For β-galactosidase marker expression reactions, transfections were conducted in triplicate using the indicated plasmids with the inclusion of 0.5 μg of β-galactosidase expression plasmid driven by a CMV promoter. At 48 hrs, the cells were extracted, cell protein was quantitated by BioRad protein dye kit, and the β-galactosidase activity was measured. The β-galactosidase activity was normalized to protein amounts and the mean results with the standard deviations are plotted. A similar result was obtained when β- galactosidase activity was normalized to the number of cells originally plated into each well. Colony formation measurements. CHO cells were seeded at 2 xlO 5 cells/well in 6-well plates and triplicate wells were transfected with 3 mg of confrol empty plasmid (pcDNA 3.1; Invitrogen), 1.5 mg of pl85HER-2 expression plasmid plus 1.5 mg of confrol DNA; 1.5 mg of pl85HER-2 plus 1.5 mg of herstatin; or 1.5 mg of herstatin plus 1.5 mg of confrol DNA. At 48 hours after addition of DNA, the cells were trypsinized and diluted 1:10 into 6-well plates in the presence of 0.6 mg/ml of G418. The media was changed every two days. At 14 days, the plates were stained with crystal violet and washed. The crystal violet-stained plates were extracted by shaking at room temperature for 30 minutes with 1ml of 0.1 M sodium phosphate, pH 4.5, in 50%> ethanol. The extracted crystal violet, diluted 10-fold, was quantitated by measuring the absorbency at 515 nM. Dilutions of 10-fold gave readings of 0.1 to 1.0, which were in the linear range of absorbency versus cell number, determined in pilot studies. Measurement of the inhibition ofpl85HER-2 tyrosine phosphorylation. Cos-7 cells were plated into 6-well plates and transfected as above. Cells in duplicate wells were transfected with 0.25, 0.5, 1.0, and 3 μg of fluorescent green protein ("FGP") expression plasmid. Empty vector was added to make the total amount of DNA equal to 3 μg in each well. After 48 hrs, the fluorescent signal was quantified at a wavelength of 520 nM for emission and 490 nM for excitation using a fluorescent plate reader. Alternatively, cells were transfected with 0.5 μg of FGP plasmid, with or without 1.5 μg of HER-2 plasmid, and with the indicated amounts of herstatin expression plasmid. Empty vector was added to make the total amount of DNA at 5 μg in each well. After 48 hrs, the cells were washed twice with PBS, exfracted in 100 μl of modified RIPA containing ImM phenylmethylsulfonylfluoride and 2 mM orthovanadate. The protein concentration in the clarified extracts was determined using the BioRad protein dye kit. The extracted protein, 20 μg, was resolved using 7.5% polyacrylamide-SDS gels and analyzed as a Western blot, first using 1 μg/ ml of anti-phosphotyrosine antibody ("anti- Ptyr"). The blot was then stripped and reacted with antibodies against pl85HER-2 (anti- neu(C)). The blots were developed using chemiluminescent reagent (Pierce) and , exposed to Kodak film. (1) Ectopic herstatin expression decreased pl85HER-2 levels in transfected Cos-7 cells by decreasing post-transfection survival. Levels of pl85HER-2 were decreased about 5-fold when herstatin was co-expressed with pl85HER-2 in Cos-7 cells, compared to cells transfected with pl85HER-2 alone (Figure 9). Expression of β- galactosidase ("β-gal") activity from a marker plasmid was monitored as an internal confrol. Figure 10 shows that the β-gal activity was greatest when the β-gal marker plasmid was co-fransfected with pl85HER-2 (about 35%> higher than when β-gal plasmid was co-fransfected with empty plasmid, "pcDNA"). By contrast, the level of β-gal activity was reduced about 3-fold in cells that were co-fransfected with herstatin in combination with pl 85HER-2 and the β-gal plasmid. A similar reduction was observed in two additional cell lines, CHO and HEK-293. Additionally, a similar level of inhibition of marker-gene expression was observed when an alternative marker, fluorescent green protein ("FGP"), was examined in cells co-fransfected with herstatin and HER-2. Thus, co-transfection of marker plasmids with pl85HER-2 alone increased the level of co-fransfected marker expression, while co-transfection marker plasmids with herstatin and pl85HER-2 reduced the marker signal. The above-identified decrease in the level of pl85HER-2 and marker gene expression was consistent with either a general reduction in the level of pl85HER-2 (or marker) per fransfected cell, or with a decrease in the survival of post-transfected cells (i.e., whereby survival was enhanced by expression of pl85HER-2, and inhibited by co- expression of herstatin and pl85HER-2). CHO cells (suitable for displaying rapid growth of stably transfected cells) were fransfected, subjected to selection with G418 for 20 days, and the surviving cell colonies were stained and quantitated to directly measure effects of co-fransfections on colony formation. Transfection with pl 85HER-2 enhanced colony growth by about 60% relative to the confrol transfected cells, while herstatin, expressed alone, did not significantly alter growth (Figure 11). By contrast, co-transfection of herstatin dramatically reduced colony formation of HER-2 transfected cells by about 7-fold (Figure 11). The affect of herstatin expression on pl85HER-2 in transfected cells was further evaluated by varying the relative proportion of transfected herstatin and pl85HER-2 expression plasmids. When the herstatin plasmid was introduced at 5-fold lower level relative to the pl85HER-2 plasmid, there was essentially no effect on the level of p 185HER-2. As the amount of the herstatin plasmid was increased relative to the pl85HER-2 plasmid, pl85HER-2 levels were reduced (Figure 14 panel B) and the expression of co-fransfected marker GFP plasmid was reduced as in Figure 9. In contrast, transfection with the control GFP expression plasmid alone, which does not affect cell survival, resulted in GFP expression that was proportional to the amount of plasmid added (Figure 13). Therefore, the reduction in the level of pl85HER-2 in cells co-fransfected with herstatin was attributable to a decrease in the extent of colony formation of post- transfected cells, and not to a decrease in the level of pl85HER-2 per transfected cell. Moreover, survival of the transfected cells was dependent upon the relative amount of expressed herstatin and pl85HER-2. Stated alternatively, the survivability of pl85HER- 2-expressing cells was substantially reduced upon exposure to herstatin. (2) Herstatin expression inhibits pl85HER-2 tyrosine phosphorylation. Under circumstances where co-transfection with herstatin resulted in a 3 -fold reduction in pl85HER-2 levels, pl85HER-2 tyrosine phosphorylation signal was diminished by 25- fold. This amounts to an approximate 8-fold decrease in the level of tyrosine phosphorylation of pl85HER-2 per cell (Figure 15). As in the case of cell survival, the extent of inhibition of pl85HER-2 tyrosine phosphorylation was affected by the ratio of herstatin to pl85HER-2 expression plasmid. The greatest effect was observed at a ratio of 2 to 1 (herstatin:pl85HER-2). Transfection of the herstatin plasmid alone reduced the phosphotyrosine signal associated with an endogenous 185 kDa protein by about 2-fold (compare lanes 1 and 2 panel C). The pl85 protein co-migrated with pl85HER-2 expressed from the transfected pl85HER-2 expression plasmid and likely coπesponds to endogenous pl85HER-2. Ectopic expression of herstatin reduced the tyrosine phosphorylation of exogenous and endogenous pl85HER-2. Furthermore, the ratio of herstatin to pl85HER-2 expression plasmid affects the extent of inhibition of pl85HER-2 tyrosine phosphorylation and the level of expression of pl85. Therefore, reduced pl85HER-2 tyrosine phosphorylation was coπelated with diminished colony formation of cells co- transfected with HER-2 and herstatin. Example 13 Co-expression of Herstatin in Transfected Cells (1) Reduced EGF Receptor Levels and Suppressed EGF-mediated Enhancement of Post-transfection Cell Survival (2) Inhibited EGF-mediated EGF Receptor Down-regulation and (3) Inhibited Activation of EGF receptors as Determined by Measuring Tyrosine Phosphorylation The group I receptor tyrosine kinases include, in addition to pl85HER-2 (erbB- 2), the EGF-receptor (HER-1, erbB-1), HER-3 (erbB-3), and HER-4 (erbB-4). The affects of herstatin on the EGF receptor in CHO cells, which do not contain endogenous EGF receptors, were examined to determine if other group I receptors might be affected. Methods. Measurement of EGF receptor activation. CHO cells at 2 x 10 5 cells/well were plated into 6-well plates and 24 hrs later duplicate wells were transfected with 0.5 μg of FGP marker plasmid, with or without 1.5 μg of EGF receptor expression plasmid, and with the indicated amounts of herstatin expression plasmid. After 24 hours, the cells were twice washed with PBS, and cultured in serum-free media for an additional 24 hrs. The cultures were incubated in the absence or presence of 100 ng/ml of EGF for 20 minutes, and then extracted in modified RIP A as in Fig. 15. Approximately 20 μg of protein from each well was resolved using 7% polyacrylamide-SDS-gels and analyzed by Western blotting, first with 1 μg/ml of anti-phosphotyrosine antibody. The blot was then stripped and probed with anti-EGF receptor antibody. Alternatively, the cultures were incubated in the absence or presence of 100 ng/ml of EGF for 24 hours, and the cell extracts analyzed by Western blotting and detection with anti-EGF receptor antibody. Measurement of the effects of the EGF receptor and herstatin on colony growth. CHO cells were plated in 6-well plates and triplicate wells were fransfected with 1.5 μg of empty vector, with 1.5 μg of the EGF receptor expression plasmid, with 1.5 μg of the EGF receptor plus 1.5 μg of herstatin plasmid, or with 1.5 μg of herstatin plasmid. Empty vector was added to bring the total amount of DNA to 3 μg in each well. At 48 hrs after DNA was added, the cells in each well were collected by trypsinization and diluted 1:10 into 6-well plates in media containing 600 μg/ml of G418. The media was changed every two days and at 14 days, the cultures were stained with crystal violet. The dye from the stained cultures was extracted as above and quantified by absorbency at 415 nM. The mean value of triplicate wells was determine as a percentage of the control- transfected wells, and the standard deviation was plotted. Similar results were obtained in three separate experiments. Measurement of stable complex formation between herstatin and pl85HER-2 and pl75EGF receptors. About 100 μl of a 50 % suspension of S-protein agarose (Novagen) was incubated with no peptide, with 50 μg of TBpexl4 peptide (provided by Dr. B. Ullman, OHSU), 50 μg of intron 8-encoded peptide, or 50 μg full-length recombinant herstatin at room temperature for lhr. Each of these peptides contained an S-protein tag encoded by the pET 30 expression plasmid (Novagen). The agarose samples were twice washed with PBS and incubated at room temperature for 1 hr with 100 μg of A431 cell extract (for the EGF receptor) or 17-3-1 extract (for pl85HER-2) solubilized in PBS containing 1%> nonidet-p40 (PBSNP-40). After incubation with the cell extracts, the agarose samples were twice washed with 500 μl of PBS-NP40 and the proteins associated with the resin were eluted at 92°C for 2 min in 40 μl of SDS sample buffer. To ensure that equal amounts of the original peptides were adhered to the agarose, an aliquot extracted in SDS sample buffer was analyzed by SDS-PAGE and Coomassie staining (a 17 %> polyacrylamide gel for TBpexl4 and intron 8-encoded peptide, and alO % polyacrylamide gel for p50 herstatin). To analyze receptor binding, an aliquot eluted from the agarose was analyzed by Western blotting and detected using anti-EGFR or anti-pl85HER-2. (l)-(3) Ectopic herstatin expression decreased pl 75EGF receptor levels in transfected CHO cells, and suppressed EGF-mediated enhancement ofpost- transfection survival. As described above for the pl85HER-2 receptor, Figure 16 shows that introduction of 2-fold more herstatin plasmid than EGF-receptor plasmid reduced the level of EGF receptor, whether in the presence or absence of the ligand EGF. GFP marker expression was coordinately reduced. When the amount of herstatin plasmid was 3-fold lower than the receptor plasmid, the amount of pl75EGF receptor was similar to that in cells fransfected with EGF receptor alone (see Fig. 16, lanes 2 and 3, in comparison with lanes 6 and 7). Herstatin expression inhibited pl 75EGF receptor down-regulation, and pl 75EGF receptor activation as determined by measurement of tyrosine phosphorylation. The impact of herstatin on EGF activation of the pl75EGF receptor was examined. As shown in Fig. 17, Lanes 2 and 3, EGF induced pl75EGF receptor tyrosine phosphorylation and enhanced the phosphotyrosine signal associated with proteins of 80 and 55 kDa. In the presence of herstatin, no detectable increase in pl75EGF receptor tyrosine phosphorylation, or in the tyrosine phosphorylation of p80 or p55 kDa proteins was observed following addition of saturating amounts of EGF (Fig. 17, lanes 6 and 7). An additional known effect of EGF is down-regulation of the EGF-receptor. Figure 18 shows, as expected that after 48 hrs of treatment with EGF, the level of the pl75EGF receptor was greatly reduced (lanes 2 and 3). However, when herstatin was introduced, down-regulation of the EGF receptor was diminished as shown in lanes 4 and 5. Herstatin inhibited growth of cells that over-express the EGF receptor. The impact of herstatin in combination with the EGF receptor on colony formation was examined. CHO cells were transfected and selected with G418 as in Figure 11. When the pl75EGF receptor alone was over-expressed, there was a 40%> increase in the cell survival compared to the effects of the control (empty vector transfected cells as shown in Fig. 11). Herstatin alone caused an approximate 20% reduction in survival of fransfected CHO cells compared to the control-transfected cells. This was not significantly different from the effects of herstatin shown in Figure 11. The co-expression of herstatin with the EGF receptor reduced the survival confeπed by the EGF receptor alone. As determined above for the pl 85HER-2 receptor, this inhibition coπelated with herstatin-mediated interference with EGF activation of the pl75EGF receptor, as shown in Figures 16, 17 and 18. Herstatin Formed a Stable Complex with both pl85HER-2 and EGF Receptors According to the present invention both full-length herstatin and its intron 8- encoded C-terminal domain (expressed as recombinant peptides) bind to p 185 HER-2 with iiM-range affinity. Further experiments were performed to determine if there was, in addition to the herstatin/pl85HER-2 binding interaction disclosed above, a binding interaction between herstatin and the pl75EGF receptor (based on the ability of herstatin to interfere with EGF activation of the EGF receptor). Herstatin formed a stable complex with both pl85HER-2 and the EGF receptor. The herstatin EGF receptor interaction was examined in a "pull-down" assay using purified intron 8-encoded polypeptide identical to the C-terminus of herstatin, the full-length recombinant herstatin protein, and a confrol peptide with iπelevant sequence all immobilized on protein S agarose (Novagen). Agarose, derivatized with one of these proteins, was then incubated with extracts of A431 cells, washed, analyzed by Western blotting and detected with antibodies specific for the pl75EGF receptor. The data shown in Figure 20 show that the pl75EGF receptor was specifically associated with intron 8-encoded peptide and with herstatin, but not with the protein S agarose with or without control iπelevant peptide. For comparison, exfracts of 17-3-1 cells, which are stably transfected with pl85HER-2, were also examined in the pull- down assay. As previously seen using nickle affinity resin (Doherty et al., 1999), pl85HER-2 was associated with intron 8-encoded peptide and herstatin, but not to an iπelevant peptide. These results show that both the pl75EGF receptor and pl85HER-2 are formed a stable complex with herstatin and with its C-terminal intron 8-encoded domain. In summary, both the pl75EGF receptor and pl85HER-2 formed a stable complex with herstatin. Herstatin expression strongly inhibited colony formation of cells that over-express pl85HER-2. Additionally, herstatin reduced EGF receptor levels, and suppressed the increased cell survival provided by over-expression of the EGF receptor. Diminished cell survival was coπelated to a reduction in tyrosine phosphorylation of pl85HER-2, and to interference with EGF-mediated activation of the EGF receptor. Thus, the negative regulatory activity of herstatin was extended to a second member of the group I family of receptor tyrosine kinases, the EGF receptor. Example 14 Herstatin Blocked the Activated Akt-mediated EGF Survival Signal The protein kinase called Akt is a key regulator of cellular survival. Activation of Akt is both necessary and sufficient for survival of cells. Stimulation of activated Akt causes inappropriate cell survival, or prevents normal cell death, which has been found to occur in several human cancers. HER-2 and the EGF receptor both cause activation of the Akt survival signal whereby, according to current theory and belief, they cause oncogenic growth (Blume- Jensen & Hunter, Nature 411:355-365, 2001; Datta et al., Genes and Development 13:2905-2907, 2000; Yarden & Slikowski, Nature Reviews, Molecular Cell Biology, 2:127-137). Methods. Measurement of activated phospho-akt (activated AKT) in EGFR3T3 cells. Measurement of activated AKT (phospho-akt) was accomplished using standard Western blotting techniques, employing a commercially available anti-phospho-akt antibody (Santa Cruz). Transfection of cells was as described herein, above. Results. Herstatin fransfected control cells that over-express the EGF receptor (EGFR3T3) were further treated with EGF (100 ng/ml in DMEM) or veliicle (control) and incubated for different times to test the affects of herstatin on EGF survival (Figure 21). To test for activated Akt, cell extracts were reacted with an antibody (commercially available from Santa Cruz) specific for the activated fonn of Akt (phospho-akt). A strong stimulation of the activated phospho-akt at 20 minutes was seen after stimulation of the EGF receptor with EGF (upper panel). Significantly, in the presence of herstatin, the activated Akt signal was eliminated. The overall level of the Akt protein was constant as shown by control lanes (lower panel of Figure 21). Thus, according to the present invention, diminished cell survival was not only coπelated to a reduction in tyrosine phosphorylation of pl85HER-2 and to interference with EGF-mediated activation of the EGF receptor (as shown in the Examples herein, above), but also to blocking the EGF-receptor-meidated cell survial signal. This result demonstrates the efficacy of herstatin in blocking the activated Akt-mediated cell survival signal generated by EGF. Example 15 Herstatin Blocked the Transforming Growth Factor Alpha (TGFα)-mediated Activation of the EGF Receptor, Survival Signal and Proliferation Signal Of all growth factors that activate the HER family of receptors, TGFα is most relevant to human cancer. TGFα expression in lung, ovary, colon, and prostate cancers coπelates with poor outcome. It is believed that TGFα expression may cause more aggressive cancer by an autocrine mechanism where the tumor continually stimulates its own EGF-receptors (Yarden & Slikowski, Nature Reviews, Molecular Cell Biology, 2:127-137). Thus, the critical steps in EGF receptor activity, tyrosine phosphorylation and the survival signal indicated by Akt activation, were examined to assess the effects of herstatin on activation of the EGF-receptor by TGFα. (Figure 22). Additionally, the signal for TGFα-mediated cell proliferation is initiated by stimulation of DNA synthesis. Thus, the effect of herstatin on the TGFα-mediated stimulation of DNA synthesis was examined as a measure of its effect on proliferation. Methods. Measurement of EGF receptor tyrosine phosphorylation, the activated Akt survival signal, and the TGFa-mediated cell proliferation signal. ■ Measurement, in EGFR3T3 cells, of EGF receptor tyrosine phosphorylation, and the activated Akt survival signal (i.e., phospho-akt) were performed as described herein, above. Measurement of the TGFα-mediated cell proliferation signal was accomplished using standard methods for measuring tritiated thymidine incorporation into cellular DNA as a percentage of control values ("Normalized CPM"). Results. Figure 22 shows that in the presence of herstatin, TGFα -mediated stimulation of EGF receptor activation was inhibited (top panel), and TGFα-mediated stimulation of the Akt survival signal was strongly inhibited (lower panel) in EGR3T3 cells. Significantly, vast excess amounts of TGFα did not counteract the ability of herstatin to block receptor activation and survival signal, indicating that autocrine production of large amounts of TGFα by tumor cells will not counteract the effect of herstatin. Additionally, Figure 23 shows that herstatin blocked the TGFα-mediated cell proliferation signal. Cells that express herstatin, or confrol cells were treated with different concentrations of TGFα and tested for DNA synthesis by measuring the amount of tritiated thymidine incorporation as a percentage of control values ("Normalized CPM"). Stimulation of DNA synthesis by TGFα was blocked by herstatin expression (Figure 23). Thus, the signal for TGFα-mediated cell proliferation was blocked by herstatin expression. Example 16 Recombinant herstatin specifically inhibited human carcinoma cells that over-express HER-2 Herstatin, according to the present invention, can be used therapeutically to treat cancer, particularly cancers of the breast, ovary, lung, colon, glia and prostate. The ability of recombinant, purified herstatin to specifically inhibit human carcinoma cells that over-express HER-2 was assessed in both ovarian and breast cancer cell lines. Methods. Production of recombinant, purified herstatin. Recombinant herstatin was produced as a histidine-tagged protein in insect S2 cells using standard insect expression systems from Invitrogen. The recombinant protein was secreted into serum- free media and was present at about 10 mg per liter. Herstatin protein was purified by nickel affinity chromatography and by conconavalin A affinity chromatography. The purified protein was judged to be greater than 90% pure based on protein staining. Treatment of cancer cell lines with herstatin. Purified recombinant herstatin was added (to a concentration of 240 nM) to cancer cells lines at the time of plating, at 2 days and at 4 days. Growth and/or survival of the living cells was quantitated by the art- recognized MTT live cell assay (Hansen et al., Immunol. Methods 119:201-210, 1989). Results. Figure 24 shows that growth and/or survival of an ovarian carcinoma cell line (SKOV-3) and a breast carcinoma cell line (SKBR-3) that over-express HER-2 was inhibited by about 60%> relative to non-herstatin freated cells (left-most two bars). By contrast, the normal breast cell line (HBL-100) and the MCF-7 breast carcinoma cell line that express low levels of HER-2, were not significantly inhibited by purified recombinant herstatin (right-most two bars). Thus, the level of herstatin-mediated growth inhibition reflected the HER-2 expression level. Herstatin inliibition showed specificity toward those cancer cells that over-express HER-2 relative to normal cells or to transformed cells that express only low or normal levels of HER-2. Figure 25 illusfrates that inhibition of growth and/or survival (optical density at 600 nM) of SKOV-3 carcinoma (left-most three bars), but not normal HBL-100 cells (right-most three bars), showed a dose response to purified recombinant herstatin. Example 17 PHARMACOKINETICS A analysis of mfraperitoneally injected herstatin was performed to assess the pharmacokinetic properties of purified recombinant herstatin. Bioactive herstatin was effectively absorbed into the blood of injected mice, where it was not proteolytically degraded and was present for between one and three hours. Methods. Treatment of Nude mice with herstatin. Nude mice were injected mfraperitoneally with recombinant herstatin at 30 mg/kg (about 600 μg per animal), or with control vehicle. Two injections of this amount over a 7-day period did not result in any signs of toxicity. Analysis of herstatin in the serum of Nude mice. At various times after herstatin injection, the mice were sacrificed and the serum from the clotted blood was collected. A volume of 20 μl of the serum (about 2ml total) was diluted to 1ml with buffer, and the histidine-tagged herstatin was isolated by affinity chromatography. The samples were analyzed either under non-reducing conditions by Western blot analysis using a monoclonal antibody against herstatin (Upstate Biologicals), or under reducing conditions using a rabbit polyclonal antibody against herstatin generated in the Clinton lab (Doherty et a., PNAS, 1999). Results. Figure 26 shows the analysis of recombinant herstatin in the blood of intraperitoneally injected Nude mice, under non-reducing (upper panel), and reducing (lower panel) conditions. At the indicated times after herstatin injection, the mice were sacrificed and the serum from the clotted blood was collected, and the histidine-tagged herstatin was isolated by affinity chromatography and analyzed. The samples in the upper panel were analyzed under non-reducing conditions by Western blot analysis using a monoclonal antibody against herstatin (Upstate Biologicals). In the lower panel, the samples were analyzed under reducing conditions using a rabbit polyclonal antibody against herstatin generated in the Clinton lab (Doherty et al., PNAS, 1999). The presence of herstatin is detectable at 30 min and 1 hr after injection, but not at 3 hrs and 18 hrs after injection (Figure 26, upper panel; non-reducing conditions) The presence ofa single migrating species of herstatin at about 55-60 kDa under non- reducing conditions showed that monomeric but not disulfide cross-linked (aggregated) protein was present. Other studies have indicated that the monomeric protein is the bioactive form. The lower panel of Figure 26 illustrates the analysis of herstatin in serum under reducing conditions with a polyclonal antibody that reacts with multiple epitopes of herstatin giving strong evidence that the detected protein is herstatin. Significantly, herstatin is not proteolytically degraded in the blood, because no smaller products of herstatin were detected in either of the Western blots. A semi-quantitative analysis of the amount of herstatin in the mouse serum (determined by comparison with a Western signal from a known amount of herstatin) demonstrated that about 10-20%> of the intraperitoneally injected protein was absorbed and present in the blood for more than 1 hr, but less than 3 hrs. Most likely, as much as 90%> of the monomeric form of herstatin was absorbed, whereas most of the aggregated protein was not, based on the fact that the monomeric form was about 20 % of the total injected herstatin sample. Thus, the monomeric bioactive ecombinant herstatin is effectively absorbed into the blood of injected mice, where it is not proteolytically degraded, and is present for greater than 1, but less than 3 hrs before it is removed.

Claims: Claims: 1. A method for treating a solid tumor characterized by expression of EGF receptor, comprising administering an agent that binds to the extracellular domain of EGF receptor, wherein the agent is selected from the group consisting of (a) an isolated polypeptide having from about 50 to 79 amino acids taken from the sequence of SEQ ID NO:l, wherein the polypeptide binds to the extracellular domain of EGF receptor at an affinity of at least 10 s , (b) an isolated and glycosylated polypeptide having from about 80 to 419 amino acids taken from the sequence of SEQ ID NO:2, wherein the C-terminal 79 amino acids are present, and wherein at least three N-linked glycosylation sites are present, (c) a monoclonal antibody that binds to the extracellular domain of EGF receptor, and (d) combinations thereof, with the proviso that the agent cannot be the monoclonal antibody alone. 2. The method of claim 1 , wherein the solid tumor that expresses EGF receptor is selected from the group consisting of squamous cell carcinoma, lung carcinoma, colon carcinoma, and glial cell tumors. 3. The method of claim 1 , wherein the agent is the isolated polypeptide having from about 50 to 79 amino acids taken from the sequence of SEQ ID NO:l. 4. The method of claim 1 , wherein the agent is a combination of the isolated polypeptide having from about 50 to 79 amino acids taken from the sequence of SEQ ID NO:l and the monoclonal antibody that binds to the extracellular domain of EGF receptor. 5. The method of claim 1, wherein the agent is an isolated and glycosylated polypeptide having from about 80 to 419 amino acids taken from the sequence of SEQ ID NO:2, wherein the C-terminal 79 amino acids are present, and wherein at least three N-linked glycosylation sites are present. 6. The method of claim 1 , wherein the agent is a combination of an isolated and glycosylated polypeptide having from about 80 to 419 amino acids taken from the sequence of SEQ ID NO:2, wherein the C-terminal 79 amino acids are present, and wherein at least three N-linked glycosylation sites are present and the monoclonal antibody that binds to the extracellular domain of EGF receptor. 7. A pharmaceutical composition for treating solid tumors that express EGF receptor, comprising an agent selected from the group consisting of (a) an isolated polypeptide having from about 50 to 79 amino acids taken from the sequence of SEQ ID NO:l, wherein the polypeptide binds to the extracellular domain of EGF receptor at an affinity of at least 10 s , (b) an isolated and glycosylated polypeptide having from about 80 to 419 amino acids taken from the sequence of SEQ ID NO:2, wherein the C-terminal 79 amino acids are present, and wherein at least three N-linked glycosylation sites are present, (c) a monoclonal antibody that binds to the extracellular domain of EGF receptor, and (d) combinations thereof, with the proviso that the agent cannot be the monoclonal antibody alone, and a pharmaceutically acceptable carrier. 8. The pharmaceutical composition for treating solid tumors that express EGF receptor of claim 7, wherein the agent is the isolated polypeptide having from about 50 to 79 amino acids taken from the sequence of SEQ ID NO:l. 9. The pharmaceutical composition for treating solid tumors that express EGF receptor of claim 7, wherein the agent is a combination of the isolated polypeptide having from about 50 to 79 amino acids taken from the sequence of SEQ ID NO:l and the monoclonal antibody that binds to the extracellular domain of EGF receptor. 10. The pharmaceutical composition for treating solid tumors that express EGF receptor of claim 7, wherein the agent is an isolated and glycosylated polypeptide having from about 80 to 419 amino acids taken from the sequence of SEQ ID NO:2, wherein the C-terminal 79 amino acids are present, and wherein at least three N-linked glycosylation sites are present. 11. The pharmaceutical composition for treating solid tumors that express EGF receptor of claim 7, wherein the agent is a combination of an isolated and glycosylated polypeptide having from about 80 to 419 amino acids taken from the sequence of SEQ ID NO:2, wherein the C-terminal 79 amino acids are present, and wherein at least three N-linked glycosylation sites are present and the monoclonal antibody that binds to the extracellular domain of EGF receptor. 12. A method for targeting a therapeutic agent to solid tumor tissue, wherein the solid tumor tissue is characterized by expression of EGF receptor, comprising attaching the therapeutic agent to an isolated polypeptide having from about 50 to 79 amino acids taken from the sequence of SEQ ID NO: 1 , wherein the polypeptide binds to the extracellular domain of EGF receptor at an affinity of at least 10 s . 13. The method for targeting a therapeutic agent to solid tumor tissue of claim 12, wherein the isolated polypeptide is from about 69 to 79 amino acids taken from the sequence of SEQ ID NO: 1. 14. A method for determining the prognosis of tumor treatment in a patient for a tumor that expresses EGF receptor, comprising: (a) obtaining a bodily fluid sample from a patient, wherein the bodily fluid is selected from the group consisting blood, serum, urine, lymph, saliva, tumor tissue, placental tissue, umbilical cord tissue, amniotic fluid, chorionic villi tissue and combinations thereof; (b) measuring the amount of p68HER-2 expressed using an anti-p68HER-2 antibody-based assay, wherein the assay is selected from the group consisting of ELISA, immunoprecipitation, immunohistocytochemistry, and Western analysis, (c) measuring the amount of EGF receptor extracellular domain in the bodily fluid, and (d) determining a ratio between the amount of p68HER-2 and EGF receptor extracellular domain, whereby the higher the p68HER-2 to EGF receptor extracellular domain ratio, the better the prognosis of the patient.

Inventors: CLINTON GAIL M, CLINTON, GAIL M.
Applicants: CLINTON GAIL M, UNIV OREGON HEALTH & SCIENCE, CLINTON, GAIL M., OREGON HEALTH AND SCIENCE UNIVERSITY
Application year: 2001
Main classification: C12N
IPC codes: A61K38, A61K39, A61P1, A61P11, A61P17, A61P35, A61P43, C07K14, C07K16, C12N15