STRUCTURE-BASED DRUG DESIGN METHOD TO IDENTIFY COMPOUNDS THAT BIND TO CD81, A CELL RECEPTOR FOR HEPATITIS-C

Abstract: The extracellular loop of CD81 is a cellular receptor for the E2 protein of hepatitis C virus. A CD81 crystal structure has been elucidated and is provided for use in the structure-based design of compounds which bind to CD81 and thus block the binding of HCV. Methods such as docking and <i>de novo</i>drug design can be used.

Description: STRUCTURE-BASED HEPATITIS C VIRUS DRUG DESIGN All documents cited herein are incorporated by reference in their entirety. TECHNICAL FIELD This invention is in the field of the use of structure-based drug design methods to identify compounds that bind to CD81 , which is a cell-surface receptor for hepatitis C virus (HCV). BACKGROUND ART Chronic HCV infection occurs in about 3% of the world's population. It is a major cause of liver disease, but effective anti-HCV drugs are not yet available. In October 1998, the extracellular loop of CD81 was reported as a cellular receptor for the E2 protein of HCV [1]. It is an object of the invention to facilitate rational structure-based drug design of chemical compounds that interact with CD81 to inhibit the binding of HCV. DISCLOSURE OF THE INVENTION The invention is based on the elucidation of a crystal structure for the large extracellular loop (LEL; amino acids 113-201) of human CD81. This facilitates structure-based design of compounds which bind to CD81 and thus block the binding of HCV. The invention provides a computer-based method for identifying a ligand for CD81, comprising the steps of: (a) providing a 3D structural representation of the large extracellular loop of CD81 in a storage medium on a computer; and (b) using the computer to apply structure-based drug design techniques to the structural representation. Structure-based drug design techniques Structure-based drug design techniques can be applied to the structural representation of the LEL in order to identify compounds that interact with CD81 to block HCV binding. A variety of suitable techniques [e.g. ref. 2] are available to the skilled person. Software packages for implementing molecular modelling techniques for use in structure-based drug design include SYBYL [3], AMBER [4], CERIUS2 [5], INSIGHT II [5], CATALYST [5], QUANTA [5], HYPERCHEM [6], CHEMS1TE [7] etc. This software can be used to determine binding surfaces of the LEL in order to reveal features such as van der Waals contacts, electrostatic interactions, and/or hydrogen bonding opportunities. These binding surfaces may be used as follows: Docking Docking aligns the 3D structures of two or more molecules to predict the conformation of a complex formed from the molecules [e.g. ref 8]. According to the present invention, molecules are docked with the LEL structure to assess their ability to interact with CD81. Docking can be accomplished by either geometric matching of the ligand and its receptor or by minimising the energy of interaction. Geometric matching algorithms are preferred because of their relative speed. Suitable docking algorithms include, but are not limited to: - DOCK [9], the prototypical program for structure-based drug design. - AUTODOCK [10, 4], which docks ligands in a flexible manner to receptors using grid-based Monte Carlo simulated annealing. The flexible nature of the AUTODOCK procedure helps to avoid bias (e.g. in orientation and conformation of the ligand in the active site) introduced by the user researcher [11] because, whilst the starting conformation in a rigid docking is normally biased towards an minimum energy conformation of the ligand, the binding conformation may be of relatively high conformational energy [12]. - MOE-DOCK [13], in which a simulated annealing search algorithm is used to flexibly dock ligands. A grid-based energy evaluation is used to score docked conformations. - FLExX [3], which docks conformationally flexible ligands into a binding site using an incremental construction algorithm that builds the ligand in the site. Docked conformations are scored based on the strength of ligand-receptor interactions. - GOLD [14], a genetic algorithm for flexible ligand docking, with full ligand and partial protein flexibility. Energy functions are partly based on conformation and non-bonded contact information. - AFFINITY [5], which uses a two step process to dock ligands. First, initial placements of the ligand within the receptor are made using a Monte Carlo type procedure to search both conformational and Cartesian space. Second, a simulated annealing phase optimises the location of each ligand placement. During this phase, AFFINITY holds the 'bulk' of the receptor (atoms not in the binding site) rigid, while the binding site atoms and ligand atoms are movable. - C2.LigandFit [5], which uses the energy of the ligand-receptor complex to automatically find best binding modes. Stochastic conformational search technique are used, and the best results from the conformational sampling are retained. A grid method is used to evaluate non-bonded interactions between the rigid receptor and the flexible ligand atoms. Preferably, the docking algorithm is used in a 'high throughput' mode, in which members of large structural libraries of potential ligands are screened against the receptor structure [15]. Suitable structural libraries include the ACD (Available Chemical Directory, from MDL Inc), AsInEx, Bionet, ComGenex, the Derwent World Drug Index (WDI), the Contact Service Company database, LaboTest, ChemBridge Express Pick, ChemStar, BioByteMasterFile, Orion, SALOR, TRIAD, ILIAD, the National Cancer Institute database (NCI), and the Aldrich, Fluka, Sigman and Maybridge catalogs. These are commercially available (e.g. the HTS Chemicals collections from Oxford Molecular, or the LeadQuest™ files from Tripos). Pharmacophore hypotheses A pharmacophore (i.e. a collection of chemical features and 3D constraints that expresses specific characteristics responsible for activity) can be defined for the LEL. The pharmacophore preferably includes surface-accessible features, more preferably including hydrogen bond donors and acceptors, charged/ionisable groups, and/or hydrophobic patches. These may be weighted depending on their relative importance in conferring activity [16]. Pharmacophores can be determined using software such as CATALYST (including HypoGen or HipHop) [5], CERIUS2, or constructed by hand from a known conformation of a lead compound. The pharmacophore can be used to screen structural libraries, using a program such as CATALYST [5]. The CLLX program [17] can also be used, which searches for orientations of candidate molecules in structural databases that yield maximum spatial coincidence with chemical groups which interact with the receptor. de novo compound design The binding surface or pharmacophore of the LEL can be used to map favourable interaction positions for functional groups (e.g. protons, hydroxyl groups, amine groups, hydrophobic groups and/or divalent cations) or small molecule fragments. Compounds can then be designed de novo in which the relevant functional groups are located in the correct spatial relationship to interact with CD81. Once functional groups or small molecule fragments which can interact with specific sites in the CD81 binding surface have been identified, they can be linked in a single compound using either bridging fragments with the correct size and geometry or frameworks which can support the functional groups at favourable orientations, thereby providing a compound according to the invention. Whilst linking of functional groups in this way can be done manually, perhaps with the help of software such as QUANTA or SYBYL, automated or semi-automated de novo design approaches are also available: - MCDLNG [18], which fills a receptor binding site with a close-packed array of generic atoms and uses a Monte Carlo procedure to randomly vary atom types, positions, bonding arrangements and other properties. - MCSS/HOOK [19, 20, 5], which links multiple functional groups with molecular templates taken from a database. - LUDI [21, 5], which computes the points of interaction that would ideally be fulfilled by a ligand, places fragments in the binding site based on their ability to interact with the receptor, and then connects them to produce a ligand. - GROW [22], which starts with an initial 'seed' fragment (placed manually or automatically) and grows the ligand outwards. - SPROUT [23], suite which includes modules to: identify favourable hydrogen bonding and hydrophobic regions within a binding pocket (HIPPO module); select functional groups and position them at target sites to form starting fragments for structure generation (EleFAnT); generate skeletons that satisfy the steric constraints of the binding pocket by growing spacer fragments onto the start fragments and then connecting the resulting part skeletons (SPIDeR); substitute hetero atoms into the skeletons to generate molecules with the electrostatic properties that are complementary to those of the receptor site (MARABOU). The solutions can be clustered and scored using the ALLigaTOR module. - LEAPFROG [3], which evaluates ligands by making small stepwise structural changes and rapidly evaluating the binding energy of the new compound. Changes are kept or discarded based on the altered binding energy, and structures evolve to increase the interaction energy with the receptor. - GROUPBUILD [24], which uses a library of common organic templates and a complete empirical force field description of the non-bonding interactions between a ligand and receptor to construct ligands that have chemically reasonable structure and have steric and electrostatic properties complimentary to the receptor binding site. - CAVEAT [25], which designs linking units to constrain acyclic molecules. - RASSE [26] The LEL binding site To simplify computational complexity, algorithms for docking and ligand design will typically focus only on the binding site of a receptor - it is pointless to attempt to dock a ligand with a region in the receptor which is known not to be involved. Binding site identification is included in some algorithms (e.g. C 2 .LigandFit, the 'Binding Site Analysis' module of INSIGHT II, the SPHGEN routine of DOCK). Some manual guidance may be required (e.g. AFFINITY). Where a binding site has to be defined for the CD81-LEL, this should include amino acid residue Phel86. It may also include one or more of Leul54, Thrl63, Ilel81, Del 82, Leul85, Glul88, and Asp 196. The binding site may include the whole of helix D. The structural representation The invention involves the use of a 3D structural representation of the LEL. This may be a representation of (a) the complete LEL, (b) a fragment of CD81 that comprises the LEL, or (c) a fragment of the LEL which includes the amino acids which interact with HCV E2 protein. The structural representation is preferably based on or derived from the atomic co-ordinates cdsilei .pdb as set out herein, which represents the LEL dimer [see also refs. 27, 28 & 29]. Suitable structural representations include 3D models and molecular surfaces derived from these atomic co-ordinates. Variants of cdSiiei .pdb can also be used for the invention, such as variants in which the r.m.s. deviation of the x, y and z co-ordinates for all heavy (i.e. not hydrogen) atoms are all less than 2.5A (e.g. less than 2A, preferably less than 1A, and more preferably less than 0.5A or less than 0.1 A) compared with cdsilei .pdb. Co-ordinate transformations which retain the 3D spatial relationships of atoms may also be used to give suitable variants. Preferred fragments of the LEL whose co-ordinates can be used in the invention include amino acid residue Phel86. The fragments may also include one or more of Leul54, Thrl63, Ilel81, Hel82, Leul85, Glul88, and Aspl96. It is preferred that the methods of the invention use only one protein chain i.e. only of the monomers in cdsilei .pdb. Where only one monomer is used, it is preferred to use the first monomer (residues 113-202) rather than the second (residues 213-302). The water molecules in cdsiiei .pdb can optionally be omitted when performing the methods of the invention. The atomic co-ordinates given herein can also be used as the basis of models of further protein structures. For example, a homology model could be based on the LEL structure of the present invention. The co-ordinates can also be used in the solution or refinement of further crystal structures of CD81. The storage medium The storage medium in which the LEL structural representation is provided is preferably random-access memory (RAM), but may also be read-only memory (ROM e.g. CDROM), or a diskette. The storage medium may be local to the computer, or may be remote (e.g. a networked storage medium, including the internet). The invention also provides a computer-readable medium for a computer, characterised in that the medium contains atomic co-ordinates and/or a 3D structural representation of the LEL of CD81. The atomic co-ordinates are preferably cdsiiei .pdb or variants thereof. Any suitable computer can be used in the present invention. Testing compounds The methods may comprise the further steps of: (c) providing a compound identified by said structure-based drug design techniques; and (d) contacting said compound with CD81, or a fragment thereof containing the LEL, and assaying the interaction between them. The assay may be of a competitive nature. For example, the assay may include HCV E2 protein (either purified, or in the context of the HCV virion), such that E2 and the compound compete for binding to CD81. Compounds and their uses The methods of the invention identify compounds that can interact with CD81. These compounds may be designed de novo, may be known compounds, or may be based on known compounds. The compounds may be useful pharmaceuticals themselves, or may be prototypes which can be used for further pharmaceutical refinement (i.e. lead compounds) in order to improve binding affinity or other pharmacologically important features (e.g. bio-availability, toxicology, metabolism, pharmacokinetics etc.). The invention thus provides: (i) a compound identified using the methods of the invention; (ii) a compound identified using the methods of the invention for use as a pharmaceutical; (iii) the use of a compound identified using the methods of the invention in the manufacture of a medicament for treating hepatitis C infection; and (iv) a method of treating a patient with hepatitis C infection, comprising administering an effective amount of a compound identified using the methods of the invention. These compounds preferably interact with CD81 with a binding constant in the micromolar or, more preferably, nanomolar range or stronger. As well as being useful compounds individually, ligands identified in silico by the structure-based design techniques can also be used to suggest libraries of compounds for 'traditional' in vitro or in vivo screening methods. Important pharmaceutical motifs in the ligands can be identified and mimicked in compound libraries (e.g. combinatorial libraries) for screening f or CD81 -binding activity. Crystals The invention also provides a composition comprising an extracellular region (e.g. the LEL) of CD81 in crystalline form. The crystal can be used for diffraction studies e.g. X-ray or neutron diffraction. The crystal is preferably in the monoclinic space group P2 l (a = 31.5A, b = 77.2A, c = 38.5 A, β - 107.4°), with two molecules per asymmetric unit (Vm of 2.16 A 3 /dalton). In some embodiments, the composition may include ligands which are co-crystallised with the CD81 fragment; in other embodiments the composition may be essentially pure protein. BRIEF DESCRIPTION OF DRAWINGS Figure 1 shows: (1A) the CD81-LEL isolated chain tertiary structure, highlighting the head domain localisation relative to the N- and C-teιτninal helices ( -helices A and E, respectively), and the labelling of secondary structure elements. The two disulphide bridges are shown by yellow bonds; (IB) the dimeric CD81-LEL, showing the two subunits in blue and purple, with the helix labels distinguished by a ' symbol. The molecular two-fold axis is close to vertical, and located between the N- and C-termini of the two chains. Solid dots in the purple subunit trace an approximate path for the loops not observable in the electron density maps. (1C) A GRASP view of the molecular surface of the CD81-LEL dimer, in an orientation corresponding that of the blue subunit in panel 1A. This orientation brings α-helices C and D in the foreground; they can be recognised by comparison to panel A and by the low polarity region (white colour) in the upper part of the molecular surface. Figure 2 shows an amino acid sequence alignment of LEL segments from mammalian CD81 and other tetraspanins. A symbol indicating the secondary structure [PROCHECK, ref. 30; H=α- helix; G=3 10 helix) is shown on the top line. On the leftmost column, name and organism are indicated. The accession numbers for the sequences used in the alignment are: human CD81, NP_004347; Saguinus oedipus, CAB89875; rat, NP B7219; mouse, P35762; human CD9, NP_001760; human TSPAN2, NP_005716; mouse CD53, NP_031677; human CD82, NP_002222; human CD63, NP_001771; human TALLA1, AAF4412; human NET4, AAC17120. Amino acid sequences of CD81 from chimpanzee, green monkey, and hamster have been obtained from ref. 1. The last four sequences contain insertions which have not been included in the alignment to avoid the introduction of long gaps, located between pairs of underlined residues. In detail, the insertion stretches are: in CD82, eLMNRPEVTy and sLSVRKGFCEAPGNRTQSGNHPEDWPv; in CD63, dWEKTPs, cINVTVGc and kAi; in TALLA1, sPYFLEh, cMNETDc and tVAATKVNq; in NET4, aFGADDWNLNIYFNCt and dVINTQCGYDARQKPEVDQQIy. The conserved Cys residues are enclosed in yellow boxes. Amino acids involved in the association interface are shown in pink boxes. The residues which are different between hCD81-LEL and αgmCD81-LEL or tømCD81-LEL are marked in green or blue, respectively. Note that residue 163 is mutated in αgmCD81-LEL and in t /nCD81-LEL. Figure 3 shows: (3A) a schematic view of the location of Cysl56~Cysl90 and Cysl57— Cysl75 disulphide bridges (yellow bonds) within the head domain of CD81-LEL. The additional residues displayed are the sites of mutation observed in AGM CD81-LEL (green) and in tamarin CD81-LEL (red); (3B) the residues surrounding Tyrl27, the head domain core, including electron density for the two disulphide bridges; (3C) a molecular surface representation of the CD81-LEL dimer, showing mutation sites 163, 186 and 196 (green patches) which have been shown to affect binding to HCV E2. Phel96 is in the upper-right part of the figure. MODES FOR CARRYING OUT THE INVENTION CD81-LEL production CD81-LEL was purified from a recombinant E.coli strain as a fusion protein with the IgG binding domain of the S.aureus protein A. After purification of the chimeric protein, CD81-LEL was separated from the fusion by specific protease cleavage and further purified for the crystallization experiments. The purified protein was fully active as judged by the recombinant E2/CD81 inhibition of binding assay [1]. CD81-LEL crystallisation Crystals of CD81-LEL were obtained by mixing purified CD81-LEL at 10 mg/ml with 0.1M MES buffer (pH 6.0), 0.1M sodium chloride, and 10% PEG4000. The crystals belong to the monoclinic space group P2, (a = 31.5 A, b = 11.2k, c = 38.5 A, β = 107.4°), with two molecules per asymmetric unit (Vm of 2.16 AVdalton). Crystals were flash-cooled at 100K with addition of 20% glycerol as cryoprotectant. Whilst native crystals could be obtained easily, it was difficult to obtain suitable heavy atom derivatives for multiple isomorphous replacement techniques. Three suitable derivatives were eventually produced, based on Lu, Hg and Pt: NB: - Rmerge = ∑l Λ - <Λ> I / ∑ <Ji>, where 7i is the observed intensity and <Ii> is the average intensity over symmetry equivalent measurements. - Phasing power = Σ |F R I / ∑ | |Fpκ(obs)l - IFpπ(calc)l I, where F PH and F H are the derivative and calculated heavy-atom structure factors, respectively. - R c uiiis = ∑ I |Fp H -Fpl - IFκ(calc)l / IFpjj-Fpl, where F PH , Fp and F H are the derivative, native and calculated heavy-atom structure factors, respectively. - Rfactor - ∑ |F 0 bsl - IFcaicl | / Σ |F 0 bsl- Rfree is the same as Rfactor, but for a 5% subset of all reflections that were never used in crystallographic refinement. - FOM (Figure of merit) = IF(hkl)bestl/IF(hkl)l - Completeness is reported for all reflection and for the highest resolution shell. Diffraction and structure solving Native and derivative diffraction data were collected in house. Additional native data (at 1.6A resolution) were collected on beamline ID 14 (ESRF, Grenoble, France), on a MAR CCD detector. All data were processed using DENZO and SCALEPACK [31] and merged using the CCP4 program suite [32]. Crystallographic phases were calculated with CCP4 programs and refined using SHARP [33] and SOLOMON [34]. The resulting electron density maps allowed about 80% of the two independent molecules to be traced. Model building and inspection was based on the O suite [35]. The structure was refined using CNS [36] and REFMAC [37].5% of the unique data were used to monitor the free R-factor. The final values for general R-factor and free R-factor are 18.7% and 23.8%, respectively. The refined model consists of 176 amino acids, with 194 water molecules, at 1.60 A resolution. No residues are found in disallowed regions of the Ramachandran plot. Residues 238-241 are disordered in a A'B'loop. Analysis of the crystal structure Figure 1A shows the CD81-LEL tertiary structure, which is essentially composed of five α- helices (A, B, C, D, E) spanning residues Asnll5-Aspl37, Alal40-Aspl55, Leul65-Asnl72, Asnl80-Phel86 and Aspl89-Gly200, respectively (amino acids numbered according to the full CD81 sequence; refs 38 & 39). A short 3 10 helical segment covers residues Leul62-Alal64. The anti-parallel A- and E-helices can be seen as the stalk of a mushroom-shaped molecule, whose head domain (about 60 residues) is built by packing of the shorter B-, C- and D-helices and their intervening loops (loops are defined by the helices they connect i.e. AB, ..., DE). A DALI search [40] of 3D protein structures did not show significant structural homology of the CD81-LEL fold to any known protein tertiary structure. The dimeric assembly observed in the crystallographic asymmetric unit is shown in Figure IB. Two CD81-LEL chains assemble around a local two-fold axis, displaying intermolecular contacts mainly at the A:A' helix interface (a ' symbol distinguishes features of the partner subunit) and along the B -helix, which is in contact with the C-terminal region of the facing protomer. The subunit interface (986A 2 ) is composed of apolar residues for about 70% of its contact region. The A:A' interface segment involves primarily residues Vail 14, Ilel 19, Vall23, Glnl25, Phel26 and Gin 129, in both antiparallel helices, together with solvent mediated interactions between exposed polar side chains at the rim of the contact region. The second interface region includes residues Asnl42, Vall46, Thrl49, Phel50, Thrl53 & Leul54 which contact Leul97', Phel98', Serl99' & Gly200'. No water molecules are buried at the association interface. Bivalent molecules (such as specific antibodies) capable of recognising human CD81 have been reported to have a higher affinity than E2 for CD81 [41]. In agreement with the crystal structure, this suggests that CD81 exists as a homodimeric species at the cell surface. In the crystal structure, the distance between the D- and D'-helices is about 30A, close to the distance between antigen-binding sites in an assembled antibody molecule. The C-terminal His-tag does not appear to have a structural influence because the proximity of N- and C-termini within each chain, permitted by the antiparallel arrangement of A- and E- helices, is compatible with their topological location between TM3 and TM4 of native CD81 [39]. Moreover, due to the quaternary structure two-fold symmetry, the N- and C-termini of the two protomers fall in a restricted area, but on opposite faces of the assembled dimer (Figure IB). This quaternary organization is compatible with inter- and intra-cellular aggregation of CD81 in homo-dimeric species, through the association interface described. The overall shape of the CD81-LEL dimer is further characterized in Figure 1C, as a surface displaying electrostatic potential. Besides the localization of negative potential in the central region of the dimer, a low polarity patch is present in a surface region comprising the C- and D- helices. In the crystal packing this region is virtually solvent inaccessible, due to extended contacts with a symmetry equivalent dimer. The CD81-LEL head domain is essentially composed of the last two turns of the A-helix, the B-, C-, D-helices, the intervening segments, and the DE loop. The domain fold is firstly stabilized by two tetraspanin-invariant intramolecular disulfide bridges [1, 38, 39, 41], which originate from two adjacent Cys residues (156 & 157) and are oriented approximately in opposite directions within the head domain (Figure 3A). Their topological connections are Cysl56— Cysl90 and Cys 157— Cys 175: the first involves residues located at the C-terminus of the B-helix (Cys 156) and at the N-terminus of the E-helix (Cys 190); the second connects the BC segment (Cys 157) with the CD loop region (Cys 175). The latter region is fully exposed to solvent and specifically kinked towards the head domain core by virtue of the Cysl57~Cysl75 bridge. Of the four Cys residues, only Cys 175 is partly accessible to solvent (2θA 2 ). The head domain is built around the core residue Tyrl27, fully buried and surrounded by Leul31, Val47, Phel50, Hisl51 and by the Aspl28 ~ Hisl91 hydrogen-bonded salt-bridge. Tyrl27 OH atom is hydrogen-bonded to Hisl51 NE2 atom (2.7lA) and is 3.6lA from the Cysl90 Sγ atom (Figure 3B). The imidazole ring of Hisl51 is nestled between the two disulfide bridges, and is at hydrogen-bonding distance from Cysl90 Sγ (3.56A). A hydrogen-bonding network involving Tyrl27, Hisl51 and Cys 190 may play a role in regulating the redox properties of one or both disulfide bridges. Tyrl27 is strictly conserved between species in CD81 (Figure 2), and in all the CD9 sequences known to date; residue 191 is either His or Gin in amino acid sequences representing different tetraspanin sub-families (Figure 2). Sequence database surveys show that the tetraspanin family is composed of several sub-families (CD9, CD37, CD63, CD53, CD82, CD151 and others; ref. 38), for a total of about 160 amino acid sequences currently recognized. Among these, CD81 and CD9 families display close sequence (and thus structural) homology (23% residue identities are observed between human CD81-LEL and human CD9-LEL). Figure 2 shows multiple amino acid sequence alignments of CD81-LEL from seven different species, together with human CD9-LEL and other tetraspanins. Sequence-based dendrogram analysis indicates that, within the sequences included in Figure 2, human CD82-LEL is the one most distantly related to CD81-LEL (only 9% identical residues). Besides the clearly recognized tetraspanin four-Cys consensus motif, inspection of the CD81 and CD9 alignment in Figure 2 shows strict residue conservation at thirteen residues. Among these, four are charged amino acids (Lysll6, Aspll7, Lysl48, and Lys201), which are totally solvent exposed in the assembled CD81-LEL dimer. The remaining nine conserved residues can be divided into three structural classes: (a) Tyrl27, Hisl51 and lie 194 are buried residues involved in intramolecular interactions stabilizing the head domain and its contacts to the E-helix, as described above. (b) Vall23, Phel26, Leul54 and Phel98, which are primarily involved in subunit interface contacts, together with residues Hell9 and Phel50, conservatively substituted at the association interface. Conservation of the interface hydrophobic residues can be recognized throughout the sequence alignment of Figure 2, suggesting that the observed subunit interface has a general functional significance for both homo- or hetero-dimeric tetraspanin association related to intra- or inter-cellular recognition processes. (c) Glyl58 and Prol76, both of which display unique positions and conformations (as indicated by their Ramachandran Φ,Ψ pairs) within the head domain fold, occurring after the Cysl56-Cysl57 pair and after Cysl75. The selection of Gly and Pro at sites 158 and 176, respectively, may be required by the structural constraints imposed on the CD loop by the Cysl57-Cysl75 bridge, coding its specific kink towards the B-helix. Analysis of the available sequences indicates that tetraspanin LEL segments display enhanced residue variability, deletions or insertions in the protein segments 158-174 and 176-189, which are comprised between the two disulfide bridges (see Figure 2). The two segments span the BC loop, the C- and the D-helices and the DE hinge i.e. mostly solvent-exposed regions of the head domain (see Figures IB & 3A). The structural location and sequence variability of this protein region, as opposed to generally conserved protein interface and core regions, suggests its involvement in species- or tetraspanin-specific recognition processes. No binding of the HCV E2 glycoprotein to african green monkey (Chlorocebus aethiops) CD81 has been observed, but the LELs of human and AGM CD81 differ at only five residues: 154, 163, 186, 188 and 197 [1]. Conversely, E2 does bind to tamarin (Saguinus oedipus) CD81 with high affinity, and it differs from human CD81 only at residues 155, 163, 169, 180 and 196. Three of the AGM mutations have been engineered into human CD81-LEL and their binding properties versus recombinant E2 and anti-CD81 antibodies have been analysed in vitro [41]. Phel86 is the most critical residue affecting E2 binding, since the F186A mutation in human CD81-LEL fully impairs binding to E2 and to specific antibodies, the introduction of Phel86 in AGM CD81-LEL restores E2 binding, and tamarin CD81 displays Phel86 [1, 41, 42]. The Tnrl63Ala mutation marginally improves human CD81 binding to E2, whereas Aspl96Glu mutation decreases binding to E2 [41]. From a structural viewpoint, the tamarin and AGM mutated residues map in the head domain, with the exception of residues 196 and 197, which fall next to the C-terminus (see Fig. 3C), Leul97 being a subunit interface residue in human CD81. Residues 155, 163, 180, 186 and 188 are solvent exposed, such that their conservative substitution should not affect the domain conformation. On the other hand, the substitution of residues 154 and 169 is subject to polarity and residue size restrictions, since they occur at buried locations next to both disulfide bridges. In human CD81 Thrl63 is located in the short 3 10 segment preceding the C-helix, and Phel86 is the last residue in the D-helix. The two antiparallel helices build up a sort of narrow "canyon" sub-structure, whose floor is essentially defined by the Cys 157 — Cys 175 disulfide connecting the CD loop to the domain core. Residues 163 and 186 are located at the canyon end opposing the CD loop (Figs. 3A & 3C). The mutant AGM and tamarin residues which affect E2 binding map to the C-, D-, E-helices, and the intervening segments i.e. residues mostly comprised within the two conserved disulfide bridges and including the 179-193 sequence stretch recognised as the minimal epitope for E2 binding [41]. Conformational integrity of this region is likely related to the oxidised state of the disulfide bridges, particularly the Cysl57-Cysl75 bridge; reduction of the disulfides impairs E2 and antibody recognition experiments [41, 43]. Sequence alignments show that the identified region displays highest residue variability within the CD81-LEL subfamily (Figure 2), but also within other members of the tetraspanin family, which may bear specific residue deletions and insertions affecting the tertiary structure according to their molecular recognition requirements. The conservation in the CD81 sequences of the hydrophobic residues Ilel81, Hel82, Leul85, and Phel86 belonging to the D-helix (Fig. 2) is peculiar, since their solvent-exposed location (Figs. 1C & 3 A) should select against their conservation through species. Such an uncommon structural property, the extended intermolecular crystal contacts observed in this region, and the dramatic loss of E2 affinity related to Phel86 mutation, suggest that the D-helix region is the docking site for the viral glycoprotein E2. It will be understood that the invention has been described by way of example only and modifications may be made whilst remaining within the scope and spirit of the invention. REFERENCES (the contents of which are hereby incorporated in full herein) 1 - Pileri et al. (1998) Science 282:938-941. 2 - Further details: Rational drug design: novel methodology and practical applications, ACS Symposium Series vol. 719 (Parrill & Reddy eds., 1991). 3 - Available from Tripos Inc (http://www.tripos.com). 4 - Available from Oxford Molecular (http://www.oxmol.co.uk/). 5 - Available from Molecular Simulations Inc (http://www.msi.com/). 6 - Available from Hypercube Inc (http://www.hyper.com/). 7 - Available from Pyramid Learning (http://www.chemsite.org/). 8 - Blaney & Dixon (1993) Perspectives in Drug Discovery and Design 1:301 9 - Kuntz et al. (1982) J. Mol. 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O P P il* i|i l ^ oo oo oo o cπ LD θo io to ιt* Lπ Lτι co Ljι l lsJ i L Lπ cπ μ j co o Lo ρ l LD o Lo Lo ι p> p i o ι o l--> ι -J θ Lπ ιt* lt* lf* OO CO LO O OO O oOo p l o i oo iitf** ccoo c pp-- oooo ecnn iitl** oooo ttoo ttoo ecnn cooJ cooo ccnn cLnn LLJni ttoo ii HH oooo LLOO LLOO LLoo pp 11 LOOJ iiit**. ii o l OJ it* on L7i i P 1 co oo oo o P 1 to to o cn to P 1 oj μ p αi p CJi cπ LD LΛj cn LD ιi* oJ θJ iχi ιι^i-. uLo> ωLD oenι ωtθ ωLo mcn ccnn p σcnι tono *ιt*. iLBD θθ ι*t-* σcnι ttoo oo ^ιt* utθ ^ιi* uι to ^ ui ιi- μ m σi (» ι *. ιo co ω co p o ^ι ω uι H' P 1 P 1 P 1 H 1 P Λ P 1 P 1 P Λ H P 1 P 1 H 1 P> P> H 1 Lπ cn Lπ cn cπ cπ cπ cπ ιt* oJ ιi-. ιt* cπ ιt* i£i o p' to l oo ι oo i ιt* cπ ι cn cn cπ cn Lπ ρ l co (f* oo ! cn LD to p ι p Lπ cn o ιa ι u) ω oι m ιr) ιi* ι|i ω ι oι ω uι ι ι uι co i cn o Lo it* cn pi to to i Ln co ii-. ω P ιli θ3 m p μ (3i p u) ffl P ιt> μ (Λ θi ιt> uι oo ι cn o ι σ ι p cn co o cπ p uι p ι iD Λ ω t (Λ co co σι o σι io σι tj σι p p μ p p μ p p p p p p μ μ p p p μ μ μ μ μ μ μ μμ μ p p p p p p p p μ p p p p p p p p p p p μ μ p p p p p p p oσoooooooooooooooooooooooooooooooooooooooo ooooooooooooooooooo ooooooooooooooooooooooooooooσooooooooooooo ooooooooooooooooooo to to to lsJ to cπ ιi* cπ to to to to ιo to co oj to to tθ M to M to tθ !o pι to to tj to oj oj to lo to to t ιo ^ o θJ H cn ιo cπ co p l oo oo cπ tθ ιf^ cπ p l to <-θ Lπ cn ιl^ R to o Lo o oo t ιf* t ιt-> p ι to cn tθ ιi-. 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LD tO I- 1 ll* t ιl-. tO lf* tO -j en O _ Lo _ i .t.* L _O Lo _ L _ Ln oo p LO if* to oo oJ iti -j cn o ~J o cn cn co t Lo Lo cn l Ln if* p . o to pi io cn i cn cn cn co to cn o j i cn LD co co to o _ c _n to co Lo to co Lo p Lo o isoJ tO Loj rcnn cono ro-) nono nono tto tcπn oo ttOo ιl-. LD ιl*. LJi ιl*. > ιt* σ Lo pι ι ι υj ] cn ^ _ ι.l.* ι .f.* L _π . L _O Lθ _ p . ιt* l to o cn cn i to if* Lo co -ji p cn it* ij-. cn Lo . O it* LO O -J 1 p ω m to co oi μ p co fi p o il- iD Ui o to to cn -J i- J Lo to Ln LO LD p oo Ln p cπ p o co θJ ιf* o LD ιt* cn cn Lπ to cn p t ιt* Lπ ι co cπ Lo cn o to to it* O p i t CO LO LO co co oJ tj oJ Co co oJ OJ ιt* ιl* to to o oo o o ρ to co ω ω ra μ ω w uι oι | — ' p I — * o <—» P I — ■ Ul J- Ol Ul o αi ∞m OooD Uuii ωω moi oσii uu iioD ωco cooo mcn uθJ oo to cπ oo LD O LO J tO CO OJ LO p tO uι o ui p co ι ιo j to to to to to to to to t to to to to to to to to to to to to to to to to to P P μ p p to μ w M p p p p p μ iα μ io to io p io io p μ p μ μ μ μ μ μ μ μ μ cn Lπ cn Lπ o pι pι ts Lθ ιt* ιt* cn ιt* θJ cπ ιf* co ιi-. co ιi-. oJ to to pι to ρι -o en 1 00 LO O LO pi O O Cn -J CO LD OO O LD tO P i O LO Pi O LO OO OO J m J D D J j m m u l m ul ^ tΛ J Ul J P P ιa l Ul Ul M l ^ m ω σl U l^ ιtI ι^ o o o u ^o o ll- «) ^ ω ^ ol m ι^ ι^ ι|I Ul ^ u ω u m ul co σl ι^ ω (I -l (» ^ ιf* pι ] Lθ ιi ∞ o vo oo o ω cπ ιi ti* to pι t cn -J pι ∞ Lo ιi^ Lo oo co LJi ω ] ιi* ixo cπ ιl^ o oo to o to H to en cn ρι ι ι o co ι oo cn c cn cn to ι ι i Lπ i ιt^ Lo co to ιo ιt* oo ι co i ιi-. oo o u> oo co ιt* i pi |i* pi P P P P μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ p i P i p i P i i p P 1 P 1 μ μ μ p μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ p p p oooooooooooooooooooooooσooσoooooooooooooooo ooooooooooooooooo ooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo to to to to to to to to to to to to to to to to io io pi to to to pi to to to co OJ to to to to to to to to oJ to M p u i ui ra ui ii- w ω i w μ i ^ σi p μ co io w μ a o ui ω o o m ui ui ω i w ^ i t io iΛ io ω c io u i p vi μ p u o io kj μ j μ m iii fr ω o μ w w w ω m o i p p p oi oo P Ui ffl iΛ i ffl iΛ P P μ o ili ω iJi ^ w o J os αi m ^ ^ iD in i sJ si ^ p ω iJ co w ω ω o αi ra iD J σi o io uj LJi ιl* cπ σ-ι ιi-. i-o cπ cπ cn to ι cn o cπ Lo co ι ι i-o co ιt* co ω cn to o co iD o j θJ ι Kj ^ Lθ LD Lo to oo Lπ ^ o W cn ι oo cn l oo cn Λ on σϊ ! CD cn cn cn cn σ^ cn ι oo cn cn cn ι ∞ cn -J oo cn cn cn c^ ATOM 172 CB G N A 133 15..533 35..610 25..619 1..00 22..48 6 ATOM 173 CG GLN A 133 16. .687 35. .994 26. .548 1. .00 25. .57 6 ATOM 174 CD GLN A 133 17. ,650 36. .993 25. .926 1. .00 23. .61 6 ATOM 175 OE1 GLN A 133 17. .463 37. ,432 24. ,806 1. ,00 28. ,80 8 ATOM 176 NE2 GLN A 133 18. ,726 37. .251 26. ,669 1. ,00 29. .55 7 ATOM 177 C GLN A 133 14. .070 35. .146 27. .572 1. .00 22. .02 6 ATOM 178 O GLN A 133 14. .481 34. .985 28. .734 1. .00 24. .53 8 ATOM 179 N ALA A 134 12. ,946 35. .879 27. ,368 1. ,00 22. .25 7 ATOM 180 CA ALA A 134 12. .270 36. .531 28. .489 1. ,00 24. .32 6 ATOM 181 CB ALA A 134 11. .058 37. .332 27. .987 1. .00 24. .55 6 ATOM 182 C ALA A 134 11. .798 35. .628 29. ,623 1. ,00 23. .77 6 ATOM 183 O ALA A 134 11. .708 35. .947 30. ,819 1. ,00 27. .92 8 ATOM 184 N VAL A 135 11. .447 34. .399 29. .250 1. .00 22. .81 7 ATOM 185 CA VAL A 135 10. .918 33. .425 30. .203 1. .00 22. .24 6 ATOM 186 CB VAL A 135 9. .948 32. .446 29. .546 1. .00 31. .11 6 ATOM 187 CGI VAL A 135 8. .682 33. .122 29. .084 1. .00 29. .66 6 ATOM 188 CG2 VAL A 135 10. .546 31. .496 28. .557 1. .00 45. .64 6 ATOM 189 C VAL A 135 12. .043 32, .676 30. .843 1. .00 25, .73 6 ATOM 190 O VAL A 135 11. .880 32. .216 31. .986 1. .00 31. .63 8 ATOM 191 N VAL A 136 13. .147 32. .472 30. .135 1. .00 30. .36 7 ATOM 192 CA VAL A 136 14. .211 31, .649 30. .768 1. .00 29, .23 6 ATOM 193 CB VAL A 136 14. .704 30, .717 29, .663 1. .00 30, .54 6 ATOM 194 CGI VAL A 136 15. .653 31, .427 28. .727 1. .00 28. .12 6 ATOM 195 CG2 VAL A 136 15. .237 29, .401 30. .184 1. .00 33, .07 6 ATOM 196 C VAL A 136 15. .274 32. .433 31. .445 1. .00 32. .01 6 ATOM 197 O VAL A 136 16. .080 31. .921 32. .242 1. .00 35. .58 8 ATOM 198 N ASP A 137 15. .520 33. .683 31. .050 1. .00 29. .34 7 ATOM 199 CA ASP A 137 16. .647 34. .496 31. .487 1. .00 30. .00 6 ATOM 200 CB ASP A 137 17. .273 35, .171 30. .263 1. .00 31. .01 6 ATOM 201 CG ASP A 137 18. .536 35. .952 30. .547 1. .00 38. .12 6 ATOM 202 OD1 ASP A 137 19. .181 36. .426 29. .580 1. .00 33. .65 8 ATOM 203 OD2 ASP A 137 18. .933 36, .171 31. .710 1. .00 34, .77 8 ATOM 204 C ASP A 137 16. .127 35, .551 32. .450 1. .00 35, .73 6 ATOM 205 O ASP A 137 15, .411 36, .471 32, .043 1, .00 35, .33 8 ATOM 206 N ASP A 138 16. .486 35, .444 33. .718 1. .00 41. .35 7 ATOM 207 CA ASP A 138 16. .026 36, .345 34. .735 1. .00 44, .53 6 ATOM 208 CB ASP A 138 16, .349 35, .881 36, .159 1, .00 55, .88 6 ATOM 209 CG ASP A 138 14, .551 35 .422 36, .525 0, .00 27 .18 6 ATOM 210 ODl ASP A 138 14, .174 34, .275 36. .165 0. .00 27, .18 8 ATOM 211 OD2 ASP A 138 13, .773 36, .235 37, .082 0. .00 27, .18 8 ATOM 212 C ASP A 138 16 .457 37 .783 34, .550 1, .00 45 .63 6 ATOM 213 O ASP A 138 15. .642 38, .619 34, .905 1. .00 46, .37 8 ATOM 214 N ASP A 139 17, .659 38 .047 34, .046 1, .00 47, .04 7 ATOM 215 CA ASP A 139 18 .045 39 .434 33, .860 1, .00 47 .12 6 ATOM 216 CB ASP A 139 19 .315 39 .889 34 .534 1, .00 59 .30 6 ATOM 217 CG ASP A 139 20. .138 38, .776 35. .141 1. .00 60. .21 6 ATOM 218 ODl ASP A 139 20, .667 37, .956 34, .371 1. .00 66, .48 8 ATOM 219 OD2 ASP A 139 20, .245 38, .739 36, .381 1, .00 60, .42 8 ATOM 220 C ASP A 139 17 .980 39 .852 32 .397 1, .00 44, .11 6 ATOM 221 O ASP A 139 18, .684 40, .814 32, .054 1, .00 45, .91 8 ATOM 222 N ALA A 140 17, .165 39, .159 31, .588 1, .00 36, .40 7 ATOM 223 CA ALA A 140 17 .097 39 .640 30, .176 1, .00 34, .34 6 ATOM 224 CB ALA A 140 16 .549 38 .514 29 .305 1 .00 29 .85 6 ATOM 225 C ALA A 140 16 .124 40 .801 30 .144 1 .00 29 .56 6 ATOM 226 O ALA A 140 14 .956 40 .665 29 .725 1, .00 28 .22 8 ATOM 227 N ASN A 141 16 .528 41 .977 30 .625 1 .00 29 .81 7 ATOM 228 CA ASN A 141 15 .623 43, .101 30, .725 1, .00 30 .52 6 ATOM 229 CB ASN A 141 16 .336 44 .264 31 .435 1, .00 37 .01 6 ATOM 230 CG ASN A 141 16 .696 43 .864 32 .857 1 .00 54 .30 6 ATOM 231 ODl ASN A 141 15 .795 43 .768 33 .690 1 .00 48 .05 8 ATOM 232 ND2 ASN A 141 17 .991 43 .645 33 .062 1 .00 56 .03 7 I3 ι3 3 ι β ι3 ι3 β ι3 ι3 ι3 ^ ι3 ^ ι3 ^ ι3 ι3 ι3 ι3 ι3 3 ι3 ι3 ^ ι3 ^ ι3 ι3 ιa ι3 ^ ^ ι3 ^ ι ι9 ι 3 ι3 ι3 3 3 ι3 ι3 ^ ι3 t3 ι ι34 ι3 3 ι3 ι3 β ι3 3 ι3 i3 Ω O O O O O O Ω O O Ω O Ω O Ω O O O O O O O O O Ω O Ω Ω Ω O O O O Ω O Ω O O O Ω Ω Ω O O Ω O O Ω O O Ω O O O O O O O O O Ω g g lS K g g S g g S lS S lS lS S lS K iS iS g g K S IS K g g S g K g g g INJ t ttoO MtO tlsoO ttO ilNNOO ttoO ttoO !-) U) >iioi ιo3 aCo) o∞o ωcD θoo3 ocoo ω ω μ μ no iiβn moj vi mm iiJni Ω S O Ω Ω O Ω Ω t^ O Ω ≥i Ω Ω Ω Ω Ω -S O Ω Ω Ω Ω Ω 3 Ω Ω Ω Ω Ω Ω 2, O Ω Ω Ω 3 Ω Ω g Ω Ω Ω Ω Ω O Ω Ω Ω S O Ω 3 θ Ω Ω Ω ≥; θ Ω O Ω td N M QM Ω Ω td - Ω Ω ω > td N td α Ω ω > O U Ω td t p i t p to H to TJ TJ I-3 I-3 I-3 P3 I-3 I-3 >-3 lr l lr , LT' t L- t lr' t t <: < < < ' lτ i lr 1 Lτ' ^ a a a a a a a a K κj κ| κ| κ| ι ι<j κ; κ; κ| | ι ] ' ι i >ι ι iri L ir l L ti ι< ι<l ι<l ιl κ! Hi ιi κi κ; Lτ , ir' L t- t- | co co co co co co co co co co -d - Jd Jd Jti Jd iΛ !s ifl co co co co ω L Co co ω ιτ , iτ' F < I |' ' ' ! ' >' 1 >' ' |> | | ' ' ^ |> !-> p ι p p ι p ι P p p ι p ι p ι pι p ι p p ι p p ι p P p pι p ι p ι H p i H H p i p ι p p ι p ι p p ι p ι p ι p ι p ι p p p ι p ι ι p p ι ι p ι p o o ω Lo Lo Lo ιo w> Lo co ∞ ∞ o3 θo co oo rø oo ι -J ! ! ι ! ι cn cn cn cn cn cn cn Lπ Ln P H H H H μ pμ p μ μ p p p p p p μ μ μ μ μ μ μ μ μ μ μ μ μ μ Lπ cτi ιi* cπ ι oo ~J cn cn ιt* cπ oo co cn cn cn cπ ι cπ cn oo ι co i Lo oo Lo to pi H O LO O H O M U M P μ P IO H tθ ιt* ιt* Lπ J ιt* cn oo ι cn cπ cn ιf* Lπ o co ll*. If* p cn p cn oJ cn to o i cn it* Lo o -o oo Lo p t LD to co oJ oo p l -J cn oj ιt* LD oo cπ ιf* cn o o ιo co oj ιo cπ cn cn ιf* o o ι i Lo oo cn Lo cD p o to O O LLoo oo ccnn ccnn pi oo ccσo ~j! LLDD ]i -ji ιifi*. ιitt** ιitt** oo ccnn ccnn LLoo 33 ccnn ιLoo pp ι ccoo oooo ιi O _ LO ij-- oo o i cn co o p. cn -j cn p pi cn if* if* co oo Lo to Ln it* Ln o oo Lo to to o on if* ~o p i L0 L0 Ll lt* O -0 Cn if* L0 L0 t0 t0 il-. 0J |s0 0J 00 > lt* pi 00 tO P i 0J Pi ttOo O - -LO- -LO- O — IO OJ LJ -l -I O- m- L ■ r-i O —O ■ LD- tO I-- OJ p Ln - O —O Pi t l LO - L.n_ .tO. .LO- C_n .Ln_ oJ UJ cn o ∞ m to <o t cπ o -j ι cπ Lθ o o to tso to M to io to to tλj ω ω ω o to M to t KJ to to to to io to M to to to to to pι t W LNJ K ιt ω ω ιt* LJi cπ H o o ω ^ c c ιt* cπ ιf* cn cπ ιt* ιi-. ω ω iNθ pι M ω ω ι ιo ω Lπ ∞ cπ co i ιl-> μ μ μ μ μ μμ μ μ p μ μ μ μ μp μ μμ μ μ μ μ μ μ μ μ μ μ μ μp μ μ μμ μ μ p μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ ooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooσ ooooooooooooooooooooooooooooooσooooooooooσooooooooooooooooooo to to to to to tJ to to to ιo tθ ιt* ιi* co to to to μ w ιo u u ω p p w p to w w ω ιu w ω ω »o »j sj μ ^ ιt* uι ιo w M io t tJ io ιo ω ι\) io uι ui Ln ω ι ) u tj o o oo to cn ~J co ιf* to co o ιf* cn o ι cn ρι o p o isJ ^ ω iD iD P ia ω t o M p p iJ ii W ^ iD Ui ui p σi oo o W f- io ui ω Λ ui J iD i ω J M o iji oo co o co o o cπ cπ cn ιl^ o co oj ι oo M o cπ i Lθ ιt* o to cn ρι ι cπ pι w ρι oo to cπ o ι p i ιl-. cn oo cΛ CJ ι cn ι co Lπ ι ω ll-. L71 LD P i L LO LO lt* Cn LJ1 00 lt* OO tO O ll-. LD P i Cn t CD Cn il-. l LO OO Cn I Ln if* LO Cn L^ en ι co <on σ Co cn cΛ ! co o^ ι cn cn cn cn cn ι ∞ cn cn cn σι cn ι ∞ cn oo en 13 1 1 13 13 1 13 13 13 13 13 1 ^ 3 13 13 13 3 ^ 13 3 3 13 13 1 ^ 13 3 13 ^ 13 13 13 13 13 13 3 13 13 19 13 13 ^ 13 3 13 13 13 13 13 13 13 13 3 13 13 13 13 O Ω O O O Ω O Ω Ω O O Ω Ω O Ω O O O Ω O O Ω O O O Ω O O O O O O O O O O Ω O O Ω O O O Ω O O O O O O Ω O O Ω Ω O Ω O O O Ω o so so o ~ i α '~ i it* O Ω Ω 3 CO Ω O Ω Ω ≥i O Ω O O Ω Ω Ω O Ω Ω Ω Ω Ω Ω ≥! Ω Ω O Ω Ω g O Ω O O Ω Ω Ω Ω -3 O Ω S Ω g Ω Ω Ω Ω ≥! Ω Ω Ω Ω Ω Ω Ω Ω Ω ω α α Ω H O Ω td Ω Ω W td td ϋ Ω td td td ϋ α Ω td N td td O O Ω ω tO ω tO tO pi to p i t pi p tO t i t J- 5 Ω n n n n n n n n o i»ι )' L- , t L- | t , t , L- | t , L ιi 3 13 1 Ω Ω ΩΩΩΩΩΩaaaa Ki i Ki L LO LQ C ω ra ω td td H td ls td td d a - i - i - i - i if ! F ir t- i a a a a a τj τ τ) τj τj τj TJ Kj i-^ Ki Ki Kj Ki Ki H H H a a a a a m ω ω w ω ω ω ω ω iτj ifl >ti iti ifl ifl iιj ifl α α α i α c! α ci jd ^ fιJ 5J ? a • j ■ a a M ' |s ■ a Jd c! c; α co td td td td td td a T td aJ T td aJ co LO o ω co ω ω co co td μH μμμμ μ μ μμμ μ μμ μ μ μ μ H μ μ μμ μ μ μ μ H μ μ μ H p μ μμ μ μ μ μ μ μμ μ H LΠ CΠ CΠ CΠ CΠ CΠ CΠ CΠ CΠ CΠ UI LΠ LJI LJI CΠ CΠ CΠ CΠ CΠ CΠ W l ι ι ι cn cn cn cn ιi-. ιt* oo co ω 1 1 1 1 1 1 1 1 1 1 1 I I I I 1 O O O O O p i O O p i p i oo t cπ cπ it* to t p i o o o o o (O W M P P O O P U P P P O P O P U U J LO kJ tO U U IO U CO vl CO m l Ol Ul cn oo o co cn o to ι cn ~J ι co o ιt* _to . o _ .ι c .n . to c _π .ιf.* c .π •J ^ J Ul ω uI J l (Iι ul σ^ αl (Il ^ o ιo μ co ^D l o ω ^ o) *- p lD ω μ (* (n ol ^) o σl ^) l Ul [Jl to pi co ii-> to i cn Lo oo L Ln oJ oo oo co ifl Ui uι μ p o ω i ιo m uι fc o p j ιι ^ o P ιt* co iD io ιo σι P ι|i i>J θ tJ lo ω ιo l* i) -j iD (o to m i uι p* ιf* cn oJ ιi-. co Lo ι ρ* co o o to co co oo oo o oJ iχ> o cπ cπ cπ oJ cπ co cn Lo ] θj iD cn L\ Lo to o cn ι o ιo iNJ ιi-. ιt* ιf* OJ OJ OJ CO ιf* OJ ιt-. OJ ιl>* ιl-> ιf* ιf* ιf-. || ιf? * ιl-. ιf* lt. |l-. ιf* l[* ll ll-. *^ cn l CO O LD O LD O pι pι M LO ιf* ιf* ι|-. J ιf* OJ |t* LJl W lt-. OJ ιf* L71 I Cn cθ CO l l l l l LO l I LO CO cn Lπ <^ Lπ ιf-. co cn oo oj oo oo ιt* oo p Lri LD ιt* cπ to o ω cn co cn cn ι ι cπ -j ιχ) cn o co oo cc^π LO if* ~J it* It* M o oD o to iD co μ ra p io ^ μ j- σi ω ui ra σi t tJ Ln pi p uOJ) ^l (Lπn _il--.. p rOo O ιCτnι LUθ3 LLo O LθO LLOo ∞OO U5) CCo ιl ιsj t c _π ι^ _ c_ oj | . c _π c -π i Lθ θJ L -π l . -Jι t t ι .l.-. c _π ω ιf* cn ιt* co Lθ Lo i ιf* o cn ιt* to Lo o cπ cπ co o Lo p o cn oo U3 Lπ pi ιf* cn oJ oo o H l o ιt* co oJ mcn no ^i μ ρ ι mco i μ o D KI U co m lD O CO O ai O M lD l O M to to to to bo to to to to iso to to to iNO bo io to -i H M pi pi H pi pi tO tJ tO t M tXJ tO tO tO tO tO ISO tO tO tO tO tO tO tO KJ tO O tO tO pi Pi i pi Pi Pi O C ||_, if* CO O O l t* CO tO tO p pi J pi o ω ιo m *ι i 03 ω ifl ω tø m o o o M ω ω o σι σι uι ai ιt* l* ω ω j- uι ui L ιf * ιi* u ω -J I 00 00 LO LO O Lo o Lπ θj ιt* j o w o ρ ι to cπ ιt* ρi Lo pι J cn θJ cn LD ι ι oo Lπ o ρι co ιt* co i ιt-. co cn oo L/ι co Lo cn ιt* o cJ oo cπ O il* l Cn 00 L0 L71 Cn 00 0J i|-> tO 00 Ln LD L0 Ln cn pi C0 pi O tO O 0J pi i cn co Lo cn to o it-. Lj co cn θJ Li Lπ ρι i ιf* i Lo cn Lπ θJ cn cn to ιt* to ρι cn ι co LD ιt* ιf* co oj p μ μ μ μ μ μ μ μ p p μ μ μ μ μ p μ μμμμ μ μ μμ μ μμ μ μμ μ μ μμμ μ μ μ μμμ p μ μ i-'μ μ μ μ μ μ μ μ μ μ p ooσoooooooooooooooooooooooooooσoooooooooooooooooooooooooooooo ooooooooooooooooooooooooooooooooooooooooσoooooooooooooooooooo t tO M IO tO tO tO tO t tO tO CO CO CO Lπ tO tO NO OJ to tO tO tO tO M IO tO M tO tO lNO tO M ^ ιi* ι cn ιi*. ι cn cn cπ cπ i-o o ιl- * LD ιi* ι > cn ιl* o cπ ^ co C ιf* ιi* ιi-. -^ cπ cn cπ M co M l f-. C0 0J 00 0J L 0 pi 0J C0 0J l O L0 !O Cn 0J I O Cπ pi 0J I Cn θ pι C0 O pi ιl* -0 θ σ^ L0 Cπ LD ιf* σi ιf* ιl* L0 00 C^ π cΛ H c cn cn o π c co i ιf^ W ιi-. c w υ3 ιi^ cπ t i ρ i ιi-. if * ι t i ι c D c^ cn co cn cn ^ cn cn co cn cn ι oo cn » oo cn cn cn -J co cn cn cn cn cn cn J co cn cn oo cn cn ι co cn oo c^ Λ -I-* *. to >0 o o o Ui o LΛ o CΛ CΛ Jc JC*> ΪP*" p 3 ι3 ι O O O J-* l-> pi pi pi p O O O O O O O O O O J l0 lD L0 LD l0 LD l0 <£> LD 00 00 00 00 00 C0 00 00 C0 C0 ] l -J l l -0 ] -J Lπ ιi ω ιo ρι o LD ∞ -J cn Lπ »t ω ι>j p ι o Lo oo -J cn Lπ ιt* θJ tθ H o ω oo ι cn cπ Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω ≥i Ω Ω Ω Ω 3 Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω 3 Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω £3 Ω Ω 3 CO Ω Ω Ω tjϋ > O O Ω td tjd Ω Ω W > α α Ω ω Ω Ω td | Ω td U* o ω Ω td t pi tO i tO pi tO pi ISJ H > ι 3 ι ι i3 3 β lr l t- f t- , lr l t- i t lr l lrι >' ' ' >' p 3 ι ι p 3 >3 ι ι3 t-' t- , t t- , --ι t- | -ι t- i i3 3 3 P3 3 3 ι co co co co co co co co co co c .o co Ω Ω Ω Ω Ω _ Ω a a a a a a a a a H td td H td td td t-' t- i t- i ir' t-' a a . a .. a„ „a -a. a M td td td td td td td a ffi ffi ffi ffi 1-1-3 ffi M M M M td td td td td td td td ir 1 t- i ir 1 - i " K; " κi j pd d d jd d jd G α α c! l i-, α c| ' ' ?α ?d d ? jd jd α ci α α -i c; Jd d d |Λ |Λ ^d jd fd d |Λ pd Jd d |a ?d d fd !S !3[ ^ K! co co ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ μ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ P P P p i p i p P i μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ p μ μ μ p μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μμ μ μμ μ μμ μ cn cn <oΛ cn cn cn cn cn cn cn cn cn cn (o^ cn cn cn cn cn cn cn cn cn cn cn cn cn cn c^ ι cn cn cn cn cn cn cn υι cπ cπ cπ cπ cπ cπ cπ ιi-. ιi* ιt* ιi-. ιi^ o oj θJ co oJ co oJ to t to to M t M 1 1 p p o o o w ^ ω ^i ∞ i ui ^ fe ω iJ ω io t p o o o p iNj ω ui a ^i si Ln ii ^ iii ii- ui ui ui iii ijj p io o o μ t rf-. OJ to to P> P 1 o O OJ cπ cπ if* -o. cn cπ ρ ∞ co o to ι ι o ι cπ co oo cπ θ (D^ co Lπ j ιt* cn ιι --jj σσ ~Jι oo lι oooo ιιit^* Lθ3 LLDD Ccππ ccoo --JJ ccππ cooo cooo ρρ ii LLDD ttoo oo ccππ oo ID cn cn CO rf * 00 co so cπ to LO oo cπ o i m m μ o w oι μ ^ o θ3 σι m ∞ M ω ω i ι u ^ -j M (i i ιo uι uι μ μ ιo uι m oo t\) ω _ ^ _ u _ι i _J_ J . i.- t,_ i_sJ. (..i O 00 to cn ρ> rf * CO so o en LO o ι lo iii σi fc U ∞ o ω μ ^ ω μ ω μ αi i ω u fc μ iΛ μ o cni ^rf* oo iLoo ocoi mcn ocni iLDo iι ωoo μpι μpι ∞oo icjπ iLoD Mto iLnπ iLoθ iLDD oo ucni oji cn -o -J cπ LD -J to -J 00 cπ CO OJ OO O O OJ C OJ CO OJ I>O OJ t tO tO ISJ tO t ISJ tO tO IS tO t t LV> tJ tO t tO tO OJ OJ OJ tO M o Nj μ ι μ μ μ o o θ (B o ω o (ii j oι oι ^ tΛ θi Ni j ^ ifl i ^ (iι iD ∞ ιo μ o o co iD ι ro iD θ U) ∞ iD ω o o o μ o μ ι ω u u uι ^ ^ m i i μ ω to (- bo to p μ ι o crι αi ιt* J ) H) ιt* j |- ιi) ιt* α) u oi (» to ) P to ι o uι p p oo o ιl-. oo ιi^ cn to cn ιf* LD p ~J to p i Lπ Lo -o. cn ιt* t\J Lπ ιi-. i Lo to o Lo cn ι i Lo oo ι ι o ιf* oo pι pι ι o oo -o cπ Lo -J o to o cn co co ιf* ιf* p cn oo oj pι cn p υ_o . L _o .rf.* _to_ o_ L_D .tθ_ L_θ .ιt,_* o—o L_D o„o cn ρ ιt* cπ oo ρ pι i Lo to cn cn Lπ to w to θJ cn ω cn ιf* oo Lτι to ι cn ! θ LJi ιo t f-. μι o cn ui !t* co to cjι ρι ! Lo o cn to ∞ ω IO U W M 1 M hJ tO IO M W tJ W IiJ t K) tO tO to ω ω Kl l\) I M t0 10 1 W *O I I W t\) t kj μ p P μ μ μ μ μ p μ μ μ μ tO pi p i pi tO tJ tO tO tO ISO tO iD iD ω -o w m -J i ∞ vj j ui σi m J oi vj m ui ui il- ui i- o M S J t i i p p p ω p o iD iD ϋi ι o ι ! oo oo cπ cn oo co O LD LD LO LD pi p i p i OO OO O Lπ oo n o o p cn ι co M CD θo oo cπ μ μ μ μ μ μ μ μ p μ μ μ μ μ μ μ μ μ μ μ μ μμ μμ μ pμp p μμ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μμ μμ μ μ μ μ μ μ ooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo Ooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo > oo o co c oJ cπ ι cπ oj ιi Co i Lπ o Lo > o to pi o o ρi Lo D tri J io M ω ui co -j μ w oi P o ^ m o M ω uι ∞ o ι* tJι iso σι iD (i to uι ω uι uι oi ιt * ι* m ι μ σι to ι* ιo p o to cn co μ- oo cπ oo Lo o oo to i LD rf* cn Lπ pι o p i c cπ o LD cn o to ιt^ to to Lπ ρ ι co oo ι cπ cn oo to ι oo cπ o o j |f* ι t\o ι cn rf* to p i Lo o co co Lo cπ Lπ i LD θ oo Lπ to Lo to ~j Lπ rf* oo oo to co cn to ι ι ι co cn cn oo on cn ι oo cn cn cn cτι cn cn ι co ^ cn σι ι co cn cn co cn τι l oo cn σ^ cn c^ -j co cn cn ^3 cn cn Λ 4-* 4s- > > t to o o O o o Λ Oi ;^ ; ; i ; ' i ; ; ; ; !y ' ' ;ji > i 33333333333333333 33 33 ι3 i3 _ 3. 3 i333 i3 3 _ 3 333 33 i3 J Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω O Ω O O O Ω O Ω O Ω Ω Ω Ω Ω Ω 1313 !31313 I313 I3131313 !3131313 !3 !3 1313131313 1313131313 !3 13131313 1313 g !3 13 g 1313 1313 1313 131313 it-* l cn S Ω Ω Ω Ω td n r l r' P P r : r< P F > >> p ' > £> P i P P P P r' t< -ι - | - t- | t- | l- tι t-' t < C0 C0 C0 C0 C0 C0 3 3 3 Kj K M td W M td H td co ω ω co to ω ω m ω ω ω ω ω ω ω ω κj . lτ td H l3 H M H td ; < c α α c3 α G G ≥! g 3 S -3 S ≥! S a S S 3 S -3 S S ω ω c ω ω ω a a 33 a ; ; ; ;> : ; : ;> ; ; ;> ' ;> μp μμμ μ μμ μ H μμp μμ μH μμ j j -j j ι σι αi Λ oι oι σι m cπ ιf* ιf . !t ιf to co t i Lπ oj cπ co it-. rf* it* OJ OJ oJ OJ ii* J if-. if-. if* J OJ ii-. co co oJ OJ OJ Co oJ Co co oJ OJ Lo oJ OJ Co co oj co oj oJ U o ρ ι o ι ) i oo o o o H o o D ω o D oo o cO ιiϊ* cπ cπ c c cπ c LΛ ιi-. oo co c o j ιf-. rf* c cπ OT bo pι cπ cD ιi-. cn o H cπ cn ιt^ θJ θ Lo cn ι θJ θ pι o cπ ! co ι cn o ιi-. ιi*- cπ co o o ιi^ ιt^ cn cπ M ] ω cπ U3 Uo ιo o ω cn ιl-. o ∞ ∞ cπ to ι- j o π ω oJ ∞ ∞ ∞ p i Lo cn o -j θJ i ιt^ ! σϊ cn co <3^ o cn ιf* ιt* ρι ι θ ιt* ρ cπ cπ Lo σ^ ιt* Lπ Lπ Lθ Lπ co o co j Lθ Lπ o cn LJi Lπ co o ι-* co co M C0 M t C0 tO CO I>O 0J C0 CO 0J 0J OJ OJ 0J C0 C0 00 OJ OJ 0J C0 CO C0 CO C0 C0 ιt* OJ OJ 0J CO C0 0J 0J 0J C0 OJ Co ω o oo Lo o LD o Lo o ρ to t ! cn cn Lπ ιf* ιt* co ιi* o j ιi-. co oo ιi-- ιi-. cn cπ o Lo oo cn Lπ σι co rf* co o O rf* cπ co cπ to cπ co cn l l cn co cπ to o to LD p ι oo ) cn t>o oJ co cπ rf* co oJ o Lθ Lπ to > o rf* -o Lπ O p O pi LO CO Lπ LD OJ CO LO OJ Cπ LD tO LO il-. CO LD LO OO to o cn cπ o cn Lo cn co Lo o rf* to > o co cn o -J Ln to cn cn co oo to o i-* O LD cn io oo -i o -o. o -J O -J C _o- cn ρ θ Lπ LJi co p ι co rf* co ι pι o p rf* ρι pι cn ι cn rf* cπ ι cπ cn co co -j i co l LD i OJ o cn cn cn Ln pi oo i co Lo o j i i o cn on i cn cn Ln i cn cπ to M Lo H σi ιl*- o o to ω ι to oJ uι α^ rf* p co rf* Lo to rf* to Lri rf* cn -o co μ μ p μ μ μ μ μ μ p μ P P i p i p p i p μ μ μ μ μ μ μ p μ μ μ μ μ μμ μμ μμ μ p μ μ μ μ μ p μ p p oooooooooooooooooooo o o o oooooooooooooooooo ooooooooooooooooo o o o o oooooooooooooooo o o o o ooooooooooooooooooo oooooooooooooooooo oJ t o oJ M to r3 to oJ OJ tO Ln oo oj oo i>o co co oJ LO rf* rf* oj oJ OO it* it* oo co oo i cn il* co oo oo it* rf^ o cn o o oo Lo -o ] μ i M <Λ θ LD σι. cπ ιo ι-' tt* to i Lθ rf Lo to rf* o to cn cn to o ιf* Lo ∞ -J U to OJ OJ cπ oo J iii iΛ iii ^ oi ui iti co w m co p ω μ ∞ P oi o m w w i iΛ iΛi μ ∞ o μ o ifi cD μ i ω co oo ui io i OJ LO -j ι cn o o o to to oo LD o I O I- 1 i -J o M cn w -j t uι W ιi* ^ co o3 μ ^ ιi- o ω oι ιn o (n o iJi m ιn u iD o ) U [ μ o uι -) o σι αι ω ιt* σι μ 00 P-> lo μ μ i tj o μ σi ^i w σi ^ co cn cn cn cn cn cn ι co σι ι co σ^ cn cn ] co cn ^ oo cn cn cn ι oo oΛ l cn cn cn cn cn ^ oo cn cn c^ 3333333333333333333333333333333333333333333333333333333333333 OΩΩOΩΩΩΩΩOΩOΩOΩOOΩΩΩΩOOΩΩΩOOOOOOΩΩOOOOΩOOOOOOOOOOOOOOΩΩOOΩOO O gggSKSggggggKKlSlSSKKgKgKgSSlS K LΠ CΠ CΠ CΠ CΠ CΠ CΠ LTI LΠ LJI CΠ CΠ CΠ LΠ CΠ LJI LΠ LJI LJI LΠ LJI LTI LΠ LΠ CΠ CΠ CΠ CΠ CΠ CΠ CΠ CΠ OO O C C C OJ OJ OJ tO tO tO t tO NJ tO I>O tO IO Pi p p p p p M O O O O O O O O O O LD LD LO LO l I LD LO LO LD LD CO O OO M ι cn cπ ιi-. co to θ LD Co ι σι cπ rf* co tθ θ LD θo ι cn Lii rf* Lo to H o υo co l cn cπ ιl* oJ to ι-ι o vD ∞ Ω Ω S Ω Ω Ω Ω Ω S O Ω Ω Ω Ω Ω Ω S Ω Ω Ω Ω Ω Ω Ω ≥I Ω Ω ≥J Ω Ω Ω Ω ≥J Ω Ω Ω Ω Ω ≥I Ω Ω Ω ≥: Ω Ω Ω Ω Ω ≥! Ω Ω Ω Ω Ω Ω CO Ω Ω Ω Ω tϋ Ω td O Ω Ω Ϊp ϋ Q O B > O O Ω td p y Ω M Ω td Ω ω | O Ω td p i H t μ μ to to p i ! tO L C C CO C H H H H H H H H H H H H H H H H ω co co ω co ω Ω Ω Ω Ω ω ω co ω ω co τj τj iχi τJ TJ TJ TJ Ω Ω Ω Ω Ω rΛ Oι ra H B H H H M ^ L- i L P L L- 1 t t t i L- i t- | ι , - P _P w -- .n_ w ω tD tΛ tn ω H H H H H t - H td H H td td Jd j jd jd ?d d d κ; κ! κ; κ; κ; S lg g Jd Jd ^ a a d td M td td K M la H td M td td M jd ? " d ' " a ' Jd ' Jd Ω Ω Ω Ω Ω Ω Ω CO CO CO CO CO ^ P ^ P ^ ^ ^ P ^ ^ ^ ^ ^ ^ P ^ ^ ^ > > ^ ^ »> $2 !> p p μ μ μ μ μ μ μ μ μ μ p p μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ p μ μ p p μ μ μ p μ μ p μ μ μ μ μ μ μ μ P i μP i p i pp μP i μp i μp i μ oo oo oo oo oo oo oo oo oo co 0oo0 0-o0 Cu0o 0υ0o Cu0 0ϋ0o CoOo 0o0o 0o0o 0o0o Cc0o CoOa ∞oo CcOo CcOo CoOo CoOo C0 C0 00 00 00 00 ^ I I l ^ ! l I l ^ l l l ^ l l -J ^ I -J l ^ l -J - -OJ --OO ^ J] l] rf* rf* rf* CO OJ OJ OJ OJ OJ tJ MtO ktOj wtO wt MI lt wtO pp i Hp μp μp i μpi pp i μp i μp i oO oO o o o o o o io iD io iD io io m co iD iD i j i j i i m m σi m m iJi m ui ui ui ui ui ι ι ι ι ι ι ι ι ι ι ι I I I I ιt* cn l co co l Lθ Lθ LD Lo LD o p i O O p i O O O p i ι^ θJ Co co cπ oo pi |f^ θJ cn ιf^ 3 cn ι cπ J θ θ Lo l-- 1 θ Lo cn LJi Lo cn Lπ Lo -θ ιt^ co to pι -J ιi* M cπ cn t ιt* co oo -j co o oo cπ i Lθ LD to cn ι > co ι cn co cn co o o cπ cn cn cn Lo -j -J oo o ιi^ -o ι-- 1 ^ rf* cn cπ H Lo to oo o σ co to cπ oo o Lo Lπ cn o i rf* o ^ ρι co cn LD Lθ LD θ oo o Co Lπ ! rf* oo LJi l Lπ o ι p j oo ci M oι cn rf* ω .c o o tθ to to to to oo Lo o μι w ω Lπ ιf. Lπ ! cn Lπ cπ ι ∞ ∞ o LD LD θ Uo o to μ t\) ω w co ω ω o Lo u σι -J io ^ ω ϋι ^ ι*J iJ ^ θ3 ffl o ω ^ o si σι ^ ιt* LD (» o t μ o ω ι uι ιθ i p* Cn tO Cn p rf* pi CO OJ rf* tO OJ CO l 1 0J Cn LD CO l o o ιt * o ^ ra o \ μ o ω m ^D U) Ul ιo ^ M co ι^* -J o <Jι o to l μ o o μ ^o ol lJ ^) co ul Ul σι σι ^* ω ~J rf* rf* co Lπ o cn ιt* Lo to ρ ι o co cn ~J rf* oo co cn -n to o m ^] ^D ^D l ω ιD l^ Ul (i lt* (!l O Ul μ o σl W Ol O •J l μ l ) ιt* J l ω ι]l co ω l Ul lo ol O t α) ^ μ cπ ιt* p cn l>o ρ ι to o cn -o p ι o o co l to p ι o oo co NJ M tO M M tO M M tO tO tO CO OO tO OO to OJ tO tO OJ CO CJ CO CO CO IO OO CO OO CO OO CO OO OJ OJ tO ^ σi cn -J Lπ cn LD C0 CD 00 l C0 Pi H L0 O L 0 O 00 LD O O tO O p l > O pι tO !O t\0 pi H lλJ p 00 L0 O O pι pι tJ t0 Pi t0 0J 0J W oi o μ ^ co μ ! θ Lθ J LJi cn θ ιf* ω Lπ to ω cπ ] i*o co ι-- ω ι>o cn ∞ cn H W ro oo o cn ιl* cπ ] θ J LD cn L7i p ι i ρ μ ^ι cwπ p m O T Ccoo oo ι^i* Lωo cuπi rfιt** Jι oUo rfιf** LiDD *ιt*. iι cuπι LiDD iι ttoo ιωo Hp ιι cijπι cσn> ιιit^* ucπι ccoo oo cσnι ^ιt* ωoJ ito p μ μ o-o ccnn ccππ rf it^* -J co co oo ω ι o cπ cn to oo oo Lo o o oo oo cn ι co ιθ (o^ ιl^ rf* cπ cn rf* rf* t* ιi*. cn co co o t*o o cπ p* ω μ μ μ co oj ~-j o ιt* cπ oo to cn cn Lo cn ~J o cn -J Lπ Lo oj θJ μ μ μ μ μ μ μ μ μ μ μ μ p μ μ μ μ μ μ μ μ μ μ p p p μ μ μ μ μ μ μ μ μμ μ μ μ μ μ μμ μμ μ μ μ μ μ μ μ μ μ μ μ μ ooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo ooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo θJ to to θJ to cπ ιi-. co oJ co oo rf* oJ θJ θJ Lo ω co oJ cn co cπ ιl*. co ιt* ιf^ co ιf* ιt^ cπ ιf^ co oj θJ rf^ (o ιo ιo o o o o o M kJ u μ ω ω ιn ιo μ ιo co μ i o ω ιn o μ ιo o ι o ω ω J ca μ ι^ ιn iD i co ι ^ w w ∞ iΛ m ιn o iD θD iD o ιo J i ^ ω oo o o Cn if 00 ll-. l i(. 00 p J O O M Ul LD rf tO l£i pi LJl LJl O p Ln 0J O O lO l p i L0 ] cn LD it* O I-^ iD W w ω ω ω ^ j m o μ M ω o to ιii o σι ω *ι a uι co p m ω o uι t ^ μ ω ω ιιι ~j M σι ιt* uι co o ιt* P ∞ cn cn --J θo cn co σ^ cn ι co cn cn cn cn cn cn ι co cn cn cn cn cn ^ ι co cn ] oo ιo (n cn ^ Λ LΛ 4-* 4^- OO to t o LΛ o LΛ O LΛ © LΛ © LΛ LΛ 3333333333333333333333333333333333333333333333333333333333333 ΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩ ΩΩΩΩΩΩOΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩ UI CΠ CΠ CΠ CΠ CΠ CΠ CΠ CΠ CΠ CΠ CΠ CΠ CΠ LJI CΠ CΠ CΠ CΠ CΠ CΠ CΠ CΠ CΠ CΠ CΠ CΠ CΠ CΠ CΠ CΠ LΠ LΠ CΠ CΠ CΠ W lO to ^ LO LD LO LO LO LD OO OO OO rø CO ∞ CD ∞ 00 00 ! l ! l l -J l l l ] Cn cn Cn cn m σϊ co -j cn σi ιf-. oo ^j θ Lo ∞ ι cn cπ ιf* co j p' o ι ) co ι cn cπ ιf* co to ρ o U3 co ι cn cπ ιi^ co to H θ Ω Ω Ω Ω ≥l CO Ω Ω Ω Ω ≥! Ω Ω Ω Ω Ω Ω Ω ≥i Ω Ω Ω Ω Ω Ω Ω Ω g Ω Ω ≥i Ω Ω Ω Ω Ω ≥S O Ω Ω Ω Ω Ω Ω Ω Ω Ω -S Ω Ω Ω Ω Ω Ω Ω -Ξl Ω Ω 3 Ω Ω α a ω Ω td D O Ω d td td D Ω W ■ N- > M- " O " Ω M td ■ " H M ϋ α Q W ^ o α Ω a < α α Ώ t to M to I- 1 M p to μ> to i tO p a HaH HaHaHΩKjΩKj KΩj KjΩKl M d M M ω o co co m co co ω co ω ω co m co G G α G g g a ≥i S μ μ μ pι-* μf- μh- μh- μι- μι— h- μh- μι— μi— μi— μi- μt— μi—- μi—• i—- μ μ μ μ μ μ μ μ μ μ p μ μ μ μ μ μ μ μ p p μ μ p p μ μ p p μμ μμ μμ pμ pμ μμ μμ μμ μμ μμ μμ pμ μμ LO LD LO LO ^ l-O LO LO LD LD LO OO OO CO CO CO CO CO OO OO OO OO OO CO CO OO CO CO CO CO OO CO OO OO CO CO CO CO OO OO OO CO μ p μ pι o o o o o o ι ι ι ι iΛ Lo Lo Lo LD Lo oo oo oo oo oo oo oo oo oo -o ι -o ! ι ι ^ -j ι cn cn cn OT σι cn cn cn cn c^ i i i i i i j i i I I I I I I I I I I I I I I I I I I I I I I I I I P* l p p i P' p p i p i p p i | | l l l l I I I I I I I I rf* CO to p p* pi t p O O p i p i p i O O P i to oo rf* ιi-. ι cn cn cn cπ cπ σι cn cn Lπ cn ι ι cn ι oo o LD ιt* ιt* co oo ιo to pι o Lθ Lo oo cn oo -j oo oo ι cn cn oj oj oj oo cπ to co ιl^ i cπ oJ Lo o LJi J ιt^ ι oJ o θJ θ to cπ LD Lθ Li cn σι Co co ι o o co Lo co Lπ cπ oo co cπ M cπ cn co oo Lo oj oo cn cn cπ cn oJ cn ιl-> -o ι oo ιo co Lθ Lo ω ω to cπ o o ] cn cn oo >l^ P> t H Cn θ CO LO I>O Cn LIl l l -J C O O 1 0 rf* ιf* 0 l lO rf* CO Cθ σi O LO LO LI I H LO CO Cπ CO rt* LO O co co oj c oo co oJ Oo co oo oJ OJ OJ OJ Co oJ Co co cJ Co co co c co co co oJ OJ OJ L rf* ii^ oJ OJ OJ OJ OJ OJ Co co oJ Co rf* rf* ii cn n co oo oo ^ i on i c it* OJ if* if-. cn cn ! c i i£i co i i c c cn c o i-o o^ ω oJ i θJ ι p Lπ cπ co co oJ cn co oo Cn cn ιl*- ω C0 H t0 LD cn Lπ l--i 0J L0 L0 C0 Lπ to o cn Lo ρ* oo σι ιo pi o ρ i ιt* ιi-. ω μ μ μ μ μ to μ μ μ μ p μ μ μ p p μ μ μ μ μ μ μ μ μ μ i to to t M t u ω io M to io to to to ^ M to io to i io to t cn cn rf* rf * cn o Lo cn cn oo ω σι o ι α) oo co ιo o ιt* uι uι m σι co iE θ θ ι^ i) u ω ι p μ μ p ιιι ω ω ω i cΛπ ^rf* ωco Mιv) uco ωiso ωcj ucπι mcn cn cπ rf*. cπ co cπ cπ cπ cπ O tO Cn LO tO l pi O -J tO OO tO OJ OJ OJ it* -J L0 0J Cn ∞ O p O C0 L0 l(* J C0 C0 1-0 CD t0 I L0 C0 L0 H C0 C0 0J 0J O ll-> O Cn 0J C0 tO C0 O i rf* if* co o to p en LO tO LD O LD Lπ o oo CO ppi i oo -J o cπ o to co cCnn LLoD cCoO oO oO cCoO p|-i 1 LLDD rfιl-*. p i | (f**. |μ-* σCnι LO) uLπι LiDD tiOo Oo Luπι θo μp tto L-D ~J - rf..* L —O O _O_ CO tJ OO O LD O l ~0 -J O co rf* cπ co p* OJ to cn p LO cn ρι to cn cπ oo o ρ* to to p i rf* Lo co Lo oo cπ ιf* rf* o Cπ OJ LD LO OO O LO rf* LO tO LO rf* OJ Ul Cn t LO -J O CO O cn cn co o o μ μ μ μ P 1 P I P P P P μμ μ p μ μ μ μ μ μμ μ μ p p pμ p μ μp μ μμμμ μ μ μμμ p p μ μμ pμ μ p p p p i P i i o o o o o o o o o o o o o o o o o o o o o o o o o σ o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o to ιo bo to to cn cn cn ρι tθ ω co oi j ui rf* to rf* pι o p* cn cn cn cn ^J cn cn co cn cn ι co cn oo oo cn cn cn ι oo cn oo oo cn cn cn σ ! co cn ι cn cn cn cn σι ^ oo cn cn cn σι cn cn cn cn c^ >' ' " ' ι ^ l ι ι ' ι ' ' J | t | < ^ 3333333333333333333333333333333333333333333333333333333333333 Ω Ω Ω Ω Ω Ω Ω O Ω Ω O Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω O Ω O Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω cn cn <θN cn cn cn cn o^ cn cn cn cn cn cn cn c^ cn cn cn cn cn cn cn cn σ^ cn cn cn σ^ (o^ Lθi cπ Lπ Lπ cπ cn cπ Lπ cπ cn ιi-. ιf* !t* rf* rf ιi* rf* ι!* ιf* ιi. Lo ω ω oj ω ω co ω Lo co ι cn Lπ ιf-. LΛ) to θ Lo co ι cn cπ ιf^ oo to pι o LD co ι c^ cπ ιt* co M P* o LD θo ι cn LJi ιl* oJ io ^ Ω Ω Ω Ω Ω __! Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω g Ω Ω Ω Ω Ω Ω Ω ≥I Ω Ω Ω Ω Ω Ω Ω g Ω Ω ≥l Ω Ω Ω Ω Ω ≥l Ω Ω ≥! Ω Ω Ω Ω Ω -3 Ω Ω 3 Ω S D d Q B ϋ O Ω d O O Ω W > O ϋ Ω W Ω Ω M ^ N H ϋ O tO td H D Ω M a α tO p i tO P i , tO p i to p μ p u to p i to P P τj j τj τ τj " iτj a : ; : < !y ; ' Pι H H ffl a a a a a H a M M M M H M Co ω ω co co cn ra co ω co co ω co co co co t a t- , M H M M H M G a α ci a α J iτJ J T J iτJ T TJ TJ j TJ T T TJ T H M H Its' μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ p μ μ μ p μ μ μ p μ μ μ μ μ μ μ μ μμ μ μ μ p μ μ μ μ μ μ μ μ μ μ μ μ p μ μ μ μ LD LD LD LD LO t ^ VD LD LO LD VD V£> LD LD LD V£> LO LO ^ LO LO LO LO !£> LD LO LO LD LO LD !£> LO LD '^ ∞ ∞ ro oo oo oo -j ι ] ι ] ! ι -j cn cn cn cn cn cn cn cn Lπ cn Lπ Lπ Lπ LJi Lπ Lπ ιi I I I I I I I I I I I I I I I I I I I I I I I I I I I I I oi ιt * ιt* iΛi to μ o o u μ to O O pι p i |f* rf* ιt* OJ tθ pι o O O O O O p p i pi rf*. OJ rf* OJ tO p O O rf* rf* LO tO pi p i p p i pi 00 rf* CO 00 to p* o pi o LΠ cn rf* ιo uι ^ p o ^ ι co ιn i ιi* ^ uι ^ iD Ui o3 P μ ι * to ij ι ω -j μ m o ιo si ιo uι j* (* to o μ o ιιι iD oι ιt* μ ι iJ -J αi vil μp* «LD) LO C0 Ln rf* |-- CO rf* tO p VD ∞ o ω i Lπ ιt* cπ U3 co o cn tθ ιt* -J tj co co co ^ μ- co cπ co oo cπ oJ cπ co i ιt* cn pi |o oo oJ pi tø -o iD θJ θJ rf θJ to αo to o ω μ μ ^ m ιt* co (o ιo o ιi* iD co o o o to o o ^ ω .j o m ιt* oι ^ oι iD ( Ni uι o -J oι μ uι to ω ω ω co uι co m co i uι to oi ιt* iD o o m μ μ Ni ui io tt... rf*. rf*. rf==- rf=- rf**- rf*- tt=* rf=- rf*- rf-» rf^ rf*- rf=- rf=> rf*- rf=- rf=- rf*- rfi* rf=* rf=* rf-* rf-*- co co co co co co rf*. tt... co rf*- rf=- co rf*. rf*- rf*- rf-*. it-*- rf**- rf=» rf*- rf=- rf=* J co co co co co co co co co o co co co oo l t* OJ OJ OO rf* rf* on Cπ Cπ -O cn Cπ ιl-. CO ι|* OO O O tO tJ pι pι O l Cn l CO LD LD p ι O LD O θ ω θ O M pι tO H O H O LD LD ∞ ι^* o ul μ μ p ^ μ ιo m to o ιυ ■ J ω ul co ω p w μ μ l^l o ul o ι ω m l J n3 ^ ^ μ ^o ∞ ^ ) μ ^ ^ ^ μ ul μ μ u1 ( ) lΛ ) ^ o ul ^D ^* lJ J μ o μ ι > fc j cπ cπ ι μ ι o c c ι cn c ∞ c ∞ ιo LD ∞ ι o ∞ μ ι ω o c ω ι ω !!* t > co M o o ^ co p ι cπ c» oJ θJ oo ιo o o ιl. ιi* LD Lτι oJ to LΩ cn --J rf* cn cn σ-ι o -j σi Lθ ιi >ι. ∞ μ μ μ μ μ μ p* p i p μ μ μ μ μ p μ μ μ p p μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ p μ μ μ μ μ i ιo co i vi co co iD ) μ μ p o o o to o μ p o o ra ω co ω i£ io ιo μ o P uι ^ w u ω ijj ω L co ^ cfι σι oi ιt* ιt* ι iΛ) o μ p i ιt * iΛ) ιt* M ιt * cfι uι *- cn co o to co σ cπ ιt* oo Lπ cn cn oo J o cπ cπ Lπ ιi-. ιθ ιt* cπ cπ σ^ oJ σι co to oo cn oo -j p O LO OJ LD P* O IO OO tO l Cn rf* Ln -O rf* -J |- i pi tJl CO LO O O LJ1 00 o pi rf* o OoJ tt -ol LD LD Ln Ln it*. tO OJ t O --J rf* tO OJ l LO O pI CO LO |-- l LD 1 00 ~J CO rf* p OO to cn i rf* co υi Lo ι-ι cn oo oo oo Ln oo -j ] Ljθ o cn o o Lo co cn o pi co -o o o LD Ll Ln l cn CO OO pi O O LD I Cn l l -J O l l tO il* Cn l LO tO LD rf* rf* CO rf* rf* Cn O LD P* o cn co i Lπ Lji rf* cJ cn cπ ι o Lo oo rf* to rf* to cn μμpp μ μ μ μμ μ μ pμ μμ μμμμμpμμ μ ppμμ μ μμμμμμμμμμμμμμμμpμμμ μμμμμ μμμμ ooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo ooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo tO tO tO tO tO tO tO l* N0 tN0 tO I> t t>O tO L |I^ CO CO tO t I> tO t l l* l l^ l f*. C tO t tO t*^ c i rf^ cn m M cπ !λj ] OT ιt to ιi* c '-3 θ θ ! cπ ] ! cn cD Cπ o o ω o ∞ cπ oJ tθ !^ O LD OJ !O pi pi OJ LO l-O pi Ln OO LIl l l* * tO tJ O l l* * Cn cn 1 00 Pi Cn tO LD ^ OO il^ OO Cn C^ m o * j ω ^ iD ^ m μ o iD io ^ μ ω μ o ^ ιn *] o m ιxι * ] i o μ μ 'o ^ ^ (Λ t ιo μ iD o o ** o co u ιo o ^ M io μ m iΛ co σι ω o\ o o t f. p o to cn σι cn LΛ LΛ 4-* 4-*- LO to to © LΛ © LΛ © LΛ © LΛ O LΛ LΛ 3333333333333333333333333333333333333333333333333333333333333 Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω O Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω O Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω S !313 !313 !3 !31313131313 !313 !3131313 !3 !3 I3 !31313 !3131313 I3131313131313 !313 !3131313 S ~0 ~J -J -J ι ι ι ι ι ι ι ι ι ι ι i cn cn cn cn cτι cn cn cn σι cn cn cn cΛ cn cn cn ι-n cn cn cn cn cn cn cn c^ to P i i P P* μ μ o o o o o o o o o o ιo ιo ιo ifl iD ^ iD iD iD iD θJ c co co nj n] co o) co o) i ^ j ^ - j i vi Nj ι ι m m m m m m oι ^ w ^ o LO 00 cn cπ rf* oj to μ o ω o3 ^ m ui ιt* ω t μ o ιo o ι σι W ι^ ω M μ o ω oo ^ o> uι ^ ω w μ o ω co i oι iΛ ι^ u ω o ιo co j αι ui ιt*. ω t μ o 3 Ω Ω 3 Ω Ω Ω Ω t-i Ω Ω Ω Ω Ω Ω ≥! Ω --i Ω Ω Ω Ω Ω Ω Ω Ω Ω ≥i Ω Ω ≥i Ω Ω Ω Ω Ω ≥i Ω Ω ≥i Ω Ω Ω Ω Ω S Ω Ω o n o n n g Ω Ω Ω Ω Ω α α Ω ω Ω Ω a tsi d a α o Ω ω M H α α Ω B > N H D Ω B i to p tO p* to μ to μ to μ to μ > Ώ ω > to μ-> tr t s= , ' l ' ' < < < < < < < a a a a a τj τj τj τj τj τ a a a a a B a B a a a a a a a Ω Ω Ω Ω co co co o co co co co c j ι ' a a a a a B B a a B a H H H H H H H H H H a a a co ω co co τj τj τj τj τj H Kj Kj Kj Ki Kj Ki t _- i _ _ _ H M H B M a a a a a a co _3 -33 -33 -333 a a a a a a a B B B B B B B B B B B ω ω co co ω ω ω co co ω to ω ω ω ω co ω ω B B B B ω B B ω B B ω B i i ' ι ' >' ' ' i> ' ' ' >' J ' ' ' ' ' ' ι ' , to to o to to to to to to to to to to to to to to to to 1-ι pι pι pι pι p p p p pι p p p p O O O O O O O O O O O O O O O O O O O O O O O ω LD LO LO LO LD LO UO LO LO LO cn σi Lπ Lπ LJi LJi LJi cπ cπ ιt* ιf* ιf* ιt* ιt* to tθ M μ p p to to ∞ i co o μ tO 1 tO LD CO to o cπ o en oo cπ LD Lπ Lπ rf* rf* l f-. l f* l |^ rf* l |^ rf* O0 OJ OJ 0J C OJ CO 00 00 J CO C0 C J 0J C C0 C CJ rf* ι|* rf* rf* ι|*. rf* ι|^ ||* ||*. rf^ to t θ to o o Lo oo co cn ω l -o cn if* co cn cπ co co oo rf* ιi^ ιt* cπ ιt* ιt* ιf* cπ cπ cn ι ι cn cπ Cn C0 00 t C0 O LD 0-| CT*. l C3N I I-- i C0 Cn 0J Ln L0 0J C0 l>0 Ln Ln H H lt - H LD rf* 00 L^ t ι-ι ω oo oo Lo to ιt* p> Lo cn oJ oo o o ρι tJ to cπ bθ ιi** co co ιf* oo cn co cn ι co o tθ L^ cπ co cπ oo Co oo on ιt* rf* cπ on co cn ιt* ρ θJ o co oJ Cθ Lπ o cn ιo cπ cn ρι cπ co co cπ o Lo oo o cn co ιi^ μ μ μ μ μ μ μ μ μ μ μ μ μ μ p μ p p p p p p p μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ w ω ιi* ω Lπ ιt* ω ιt* ω w ιt* to M ω ω ιf* ιt* ω ω ω i- ) io co co o o crι αι σι ιi* uι ω to ω to M p o o cn cn -J i Lo co oo cn l cn cn rf* CTi cn cn cn cn cn cn Lo oo o cn ιf*. cπ ι to ιo c oJ cn ιl^ t cπ co ii^ ιi* o cn LD D cn cπ σι oJ cπ c o ιf* o ρ ρι 3 c H cπ rf^ cπ oo o cπ rf^ cn ιt* LD to ι ι iD ω co ^J Lπ co cn uo co co H ι cπ to tJ LD cn LJi to t H i ιi* ιf^ ! Cn pι l Cri Cπ 0J Cn θ rf* O 00 O lNJ l ω 00 θ ω pι C0 00 l O H H p H O H rf* 00 C0 O 00 l LD Cπ UJ Cn LD ∞ i σi p p p i p p' p p p p p p i p i p i p i p i O P i p O O O O O O O P i p i p i p i p p p p μ i p i p i p p i H H p i p i H P i p i p i p p i p i p i p p p p i p i i p i p i O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O to t to co cπ oo co to to to to ιo to to NJ to oj oo ιf* co cπ σ^ cπ cn cπ ιi* oo cn cπ co cn cπ ω LD l I l 0J LD t0 C0 L0 C0 00 C0 Cn t l C0 l l-0 O C0 C0 L0 Cn tO C0 rf* rf* Cn C0 1 O l f* LD Pi O l pi CD l f^ 0J ^ oo to oo θJ rf^ ι^ θJ ιf^ ι i Lo ι oo oo oo to o LD to cn oo oo ιt* ι-- oo oo oo o ρ co ιf*. oo cn co o ι to ρ i ι^ co o -o w oo ιt* LD cn ι o LD LD Cθ LJi cn co co oo oj -j ιi-. oJ o oj -^ ιt* cπ cn oo to to cn cn co ι-- p rf* σι σι ι oo cn ι ∞ cn cn cn ] ∞ cn o^ cn cn cn ! cn ι ∞ c-n cn cn cn cn cn cn cn l c^ cn ι c^ ATOM 721 CA LYS B 216 17..420 43..699 15,.889 1..00 30,.04 6 ATOM 722 CB LYS B 216 17. .601 44. .227 17. .320 1. .00 38. .33 6 ATOM 723 CG LYS B 216 17. .259 45. .678 17. .506 1. .00 51. .27 6 ATOM 724 CD LYS B 216 18. .220 46. .399 18. .436 1. .00 60. .50 6 ATOM 725 CE LYS B 216 17. .949 47. .901 18, .401 1. .00 41. .22 6 ATOM 726 NZ LYS B 216 18. .269 48. .521 19. .723 1. .00 56. .24 7 ATOM 727 C LYS B 216 17. .571 44. .843 14. .896 1. .00 32. .97 6 ATOM 728 O LYS B 216 16. .642 45. .376 14. .285 1. .00 32. .82 8 ATOM 729 N ASP B 217 18. .815 45. ,234 14. .662 1. .00 30. .39 7 ATOM 730 CA ASP B 217 19. .079 46. .384 13. .755 1. .00 28. .18 6 ATOM 731 CB ASP B 217 20. .615 46. .597 13. .856 1. .00 37. .07 6 ATOM 732 CG ASP B 217 20. .974 47. .025 15. .271 1. .00 50. .77 6 ATOM 733 ODl ASP B 217 20. .325 47. ,961 15. .789 1. .00 50. . 99 8 ATOM 734 OD2 ASP B 217 21. .873 46. .381 15, .855 1. .00 48. .20 8 ATOM 735 C ASP B 217 18. .710 46. .045 12, .326 1. .00 27. .65 6 ATOM 736 O ASP B 217 18, .209 46. .917 11, .586 1. .00 29. .68 8 ATOM 737 N GLN B 218 18. .945 44. .818 11, .903 1. .00 26, .96 7 ATOM 738 CA GLN B 218 18, .641 44, .363 10, .567 1. .00 26, .89 6 ATOM 739 CB GLN B 218 19. .194 43. .015 10, .137 1. .00 28. .58 6 ATOM 740 CG GLN B 218 18. .876 42. .625 8, .703 1. .00 28. .47 6 ATOM 741 CD GLN B 218 19, .550 43. .604 7, .748 1. .00 39, .80 6 ATOM 742 OE1 GLN B 218 20, .732 43. .897 7, .957 1. .00 49, .83 8 ATOM 743 NE2 GLN B 218 18, .880 44. .111 6, .730 1, .00 37, .47 7 ATOM 744 C GLN B 218 17, .118 44. .408 10, .342 1, .00 26, .99 6 ATOM 745 O GLN B 218 16. .688 44. .970 9, .349 1. .00 23. .66 8 ATOM 746 N ILE B 219 16. .361 43. .806 11, .268 1. .00 22. .98 7 ATOM 747 CA ILE B 219 14, .909 43. .777 11, .054 1. .00 22, .46 6 ATOM 748 CB ILE B 219 14, .209 42. .791 11, .998 1, .00 24, .01 6 ATOM 749 CG2 ILE B 219 14, .320 43. .076 13, .484 1, .00 23, .36 6 ATOM 750 CGI ILE B 219 12. .723 42. .606 11, .625 1. .00 27. .82 6 ATOM 751 CD1 ILE B 219 12. .575 42. .170 10, .187 1. .00 28. .39 6 ATOM 752 C ILE B 219 14, .345 45. .182 11, .118 1. .00 23. .15 6 ATOM 753 O ILE B 219 13, .418 45. .528 10, .374 1. .00 24. .69 8 ATOM 754 N ALA B 220 14, .870 46. .029 11 .972 1, .00 21, .24 7 ATOM 755 CA ALA B 220 14. .395 47. .403 12, .053 1. , 00 23. .02 6 ATOM 756 CB ALA B 220 14. .940 48. .146 13, .226 1. .00 28. .82 6 ATOM 757 C ALA B 220 14. .573 48. .131 10, .730 1. .00 23. .96 6 ATOM 758 O ALA B 220 13. .704 48. .838 10, .261 1. .00 23. .87 8 ATOM 759 N LYS B 221 15, .767 47. .916 10, .161 1. .00 25. .79 7 ATOM 760 CA LYS B 221 16, .086 48. .555 8 .865 1, .00 26, .23 6 ATOM 761 CB LYS B 221 17, .518 48. .106 8 .505 1. .00 23, .73 6 ATOM 762 CG LYS B 221 17. .859 48. .602 7, .084 1. .00 32. .18 6 ATOM 763 CD LYS B 221 19. .349 48. .458 6, .835 1. .00 48. .70 6 ATOM 764 CE LYS B 221 19, .738 48. .828 5, .402 1. .00 49. .31 6 ATOM 765 NZ LYS B 221 18, .639 48. .516 4 .446 1. .00 60, .95 7 ATOM 766 C LYS B 221 15, .111 48. .086 7 .783 1, .00 26, .25 6 ATOM 767 O LYS B 221 14, .595 48. .870 6, .961 1. .00 24. . 99 8 ATOM 768 N ASP B 222 14. .904 46. .759 7, .727 1. .00 24. .13 7 ATOM 769 CA ASP B 222 13, .988 46. .167 6, .775 1. .00 23. .28 6 ATOM 770 CB ASP B 222 13. .982 44. .670 6, .968 1. .00 26, .58 6 ATOM 771 CG ASP B 222 15. .288 43, .945 6 .671 1, .00 37, .75 6 ATOM 772 ODl ASP B 222 16. .177 44. .545 6 .002 1, .00 35, .10 8 ATOM 773 OD2 ASP B 222 15 .441 42. .777 7 .110 1, .00 31, .39 8 ATOM 774 C ASP B 222 12. .586 46. .747 6, .890 1. .00 24. .42 6 ATOM 775 O ASP B 222 11, .842 46. .970 5, .918 1. .00 25. .24 8 ATOM 776 N VAL B 223 12, .130 46. .917 8 .182 1, .00 22, .06 7 ATOM 777 CA VAL B 223 10 .773 47 .427 8 .389 1 .00 20 .51 6 ATOM 778 CB VAL B 223 10 .346 47 .208 9 .897 1 .00 19 .83 6 ATOM 779 CGI VAL B 223 8 .934 47, .819 10 .090 1, .00 20, .77 6 ATOM 780 CG2 VAL B 223 10 .265 45, .693 10 .021 1, .00 22 .25 6 ATOM 781 C VAL B 223 10 .653 48, .898 8 .031 1 .00 21 .93 6 ON LΛ LΛ 4-* LO to to © LΛ © LΛ © LΛ © LΛ © LΛ LΛ ; , ; ' ' < ' 3*' ;y ; ; , i]*< ; >' ;y 3333333333333333333333333333333333333333333333333333333333333 Ω Ω Ω Ω Ω O Ω Ω Ω Ω Ω Ω O Ω Ω Ω Ω Ω Ω Ω Ω O Ω Ω Ω Ω Ω Ω Ω O Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω O Ω Ω Ω Ω Ω Ω Ω Ω Ω O Ω Ω Ω O Ω O OO OO OO ∞ OO CO CO CO CO OO OO CO OO CO OO CO ∞ OO OO CΌ OO OO OO CO CO CO CO CO CO OO OO CD ∞ CO ∞ ∞ ^ ^ ^ u u u u u u u u u u M w ω w M M M W j ' μ μ μ μ μ μ μ μ μ o o o o o o o o o o io iD io io io io io iD iD io K o O co oi co c c to pi o ω co -j cn cπ ii-. co tυ p' O Lo oo -o cn cπ if^ oJ to O Lo oo -o cn cπ ii-. oj to p o Lo co -J cn cπ Ω ≥i Ω Ω ≥i O Ω Ω Ω Ω S Ω O Ω Ω Ω Ω ≥i Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω S Ω Ω Ω Ω Ω Ω ΩΩ Ω 3 Ω Ω ≥! Ω Ω Ω Ω Ω Ω Ω3 Ω Ω Ω Ω Ω -3 B B d Ω B ! O d Ω B > a N M d td d Ω B > N H B d d Ω B B M d Ω B i N B d Ω B tO P* to p to to p i p* to p i to pi tO H ' Ω Ω Ω Ω Ω Ω Ω Ω Ω , i ' , ' l> 3 33 33 3 TJ TJ TJ TJ Tj τj τj τJ TJ TJ TJ Ω Ω Ω Ω Ω Ω Ω Ω Ω a a t i tr< t i r< Xr l t i t< t 1 tr < f ^ CO CO CO CO CO CO CO CO K! Ki K! Ki a a a a Ki a Ki a Ki K ai t K; 1 T J T T a Tj a a a a a a a a a a a a a B B B H B M B B B B a a a 3 s 3 ≥i S co co co co co co co co co a B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B B t to to to tJ M to ιo to to to to to to o to to to to ιo to to ιo t to to to to t*o M co oo o to to to to to to to to ιo to ιo to to to to to to to to to to to to to to M to to ιo to t to M o o uJ LD LD Lo LD LD Lo i-o LD Co co co co co oo co oo ι ι ι ι -o ι ι -o -J -J ι -J cn cn cn cn cn cn cn cn c^ μ p μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ P> rf* cπ cπ cn Lo ι co l oo ι oo -J θo to θ θ LD θo cn i Lπ cπ cn i ιt* cπ cn -J ι (- j- uι oι oι ι α) CD θ o p ui ιt*. ιt* ω ω to to μ p ϋi ιi* ιt* to tj P i tθ vi ) ιt* to ω o ιπ (* ιo uι ιo αι oι uι to m μ uι oι μ σι iD uι oo ui (» uι fco μ o co ι oo p i cn o cπ LD Co cπ o ρ i Lo cn cn rf* co oo o i LD rf* o oo oo cn cn cn LD LJi ιl-. cn tθ LD cn cn Lθ Lπ ιf* cπ pι co pι ι pι μ- cn rf* p p J co LD ι o j-J μp ιUo3 θωJ i* ιt* uι -j o μ κ) i t.o ~j o o ρι cn o o o o ! co μ-' tθ !f* LD Lo pι cn cπ co co rf* to Lo to oo i Lθ LD cn cn o cn Lo -o oo Lo o i Lθ Lπ co oj rf* cπ p co cπ oj to rf* rf* oo oo ι o cn co rf* to pι to oo cπ o p cn cn cn p o o j rf* cn cπ ιt* --J rf* pι ι cn cπ cπ cπ cπ ιt* rf* ιt^ cπ cπ LJi cπ cπ cπ cπ Lπ cπ cπ Lin Lπ cπ cπ LJi Lπ cπ cπ cπ cπ cπ cjι w tO pι t tO CO ! CD O O tO t rf* C ιi-> Cπ ιt*» rf* CO IO OJ t l Cπ LJl rf l f* CO OJ tO R O LO lD Cn l Ln l C^ o oo σ^ ρι ι ι ι o cn o ιt* cn cn i ρ i ρi LD Lo H cn LD Co LJi i Li ιt* o cπ co LD ι o to ι cn o o co cπ Lθ ιi* ω θJ cπ Lo oo LJi o o LJi ω ιi^ π LD Cπ ιf*. t -θ D rf* ω rf* ι ) p |--ι co cπ π Lπ cn c U3 to ιi* oo c^ to l ^ ω ω ^ ul ^o ^Λ to ω ^Jl μ m ^o to μ ω ιD ^ p o ) o co μ lJ Ul o co ι tO l ω u tJ Uι M lD ι^ ] ) o ι^] -J -J l o ω ^ μ (Λ ω (n ^ μ μ ^o ιt* P i p O ~J OJ t0 I oo co cπ o ui ij ^ o t oi iti iD ω vi ω co m co oi o o o iD ^ ω μ j io io t w μ ω w iD o ui u μ oj μ co ω M o ω p ^ co u o σi o ^ μ i u ^i io o ω ^i t P i p i P i μμμμ μμμμ μμμμμ μ μμμμμμμ μ μ p μ μ μμ pμμpμμ μ μμμμμμμμppμμ μ ooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo ooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo M IO OJ tO rf* Cn OJ |l-. CO OJ tO IO tO OJ tO tO tO I>O tO tO tO tO tO tO IO tO tO I>O tO tO tO tO W ιt* oo o Lθ LD Ui cπ o tθ ι oo cπ ∞ -J σ^ cπ ω -o p ∞ θJ cn oJ tj to ω ιi* to ω tt cπ o -j |^ iΛ ^ ι^ ιo uι ω oι μ o ι o ιo iD μ μ μ .j ιi) ^ n3 μ i o μ o ιo o M io iD ∞ to iΛ P P ^ ιo p o t co ω ι m p o ω m ω αι ι ιo cn m ω ω ι ω ιo --J ρ ιl-. cπ o cπ ιt* Lo o cn co oo tθ ιi-. ι to LD j oo oo Lι oo oo Lo to M cn -J θθ ιii LD ι cπ oo i Lπ o ιo cn -J co o^ i co o-i cn cn cn ] oo cn oo co cn cn cn i co cn oo cn cn cn cn cn cn cn cn j' oo cn cn a^ co cn cn cn cn ι co cn ι cn cn cn cn cn ι co Ω O Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω O Ω Ω Ω Ω Ω Ω Ω O O Ω Ω O Ω Ω Ω Ω Ω Ω O Ω Ω O Ω S!3g!3K!3K !3Ki3gK:3g!3 K!3 i3K!3 gi3!3i313 ;313 i3:3 LD LO LO tø OO OO OO OO CO CO OO OO OO CD OO OO OO OO CD OO OO OO OO OO OO CO OO Cσ CO OO OO CO CO CO CO OO CO ∞ o o o o 3 LD LD <£i LD LD LD i^ Lo LD oo co co oo co co oo oo co oo -j ι -o -j ι ι j ι ---α -o cn σι cn cn cn cn cn cn c^ OJ to p* o Lo oo l m cri il-. oJ to pi o Lo oo i cn Ln it* U) to pi o Lo oo -J cn ui if^ co to pi o Lo ra Ω ≥i Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω ≥i Ω Ω Ω Ω Ω Ω ≥i Ω Ω Ω Ω Ω ≥i Ω Ω Ω ≥i Ω Ω ≥i Ω Ω Ω Ω Ω s o n ≥i Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω ≥i Ω Ω Ω d d Ω B d d Ω B i Ω Ω Ω Ω B > B B B d Ω B ! ϋ d Ω B B to tO Pi o H a to p i a to a p* a Ώ a to p* t i ι | ' |jy » i ' <; ;< < < < < < < < < 1 J> > ΩΩΩΏΩΏΩΩΏΩΏΏΩΩΩΏΩ a a a a a a a a > co co ω ω co co co co co co co co co ?* 1 B M B B B B B H s; -_; !3 3 --l TJ T J J T T a > a a > a > > > > > a a a a > a a a a > a > a a a a a a > a a a a a a a a a a a a a > a > a i it* ≥i 2. ≥i ≥i ≥i ≥i ≥i ≥i a ≥i ≥i a ≥i α a α α a α α α < Ω Ω a w w a a B ro w B ro ro a B w w B w w B tj W B w a B w a B B a a a a a B a a a a a B B B B B a a B B B B B a B B B tO IO tO tO tO tO W tNO NO tO tO tO IO tO tO tO W M tO tO tO M tO t M tO W tO tO IO bO tJ tO tO ιf^ ιl_. rf* rf* ιf^ oJ θJ J θJ θJ θJ θJ co oj cJ Co co co co co co oJ Co co co co oJ Co oJ θo oo oo co Lo co co oJ Lo ω to to t to to -j -o ι ι ι ι -o i (θ^ cn σι ι3-ι m cn cn cπ cπ cπ cπ cπ cπ cπ ιi*. ιi* rf* ιi* ιi^ I-* ρ pi ρ ι o o o I I I I I I I I I I I I t t rf* 00 tO J rf* tO tO t to t H o to o o o o ^ to to o o o o o pι cπ co ιt* ιj-=. oo oo rf* oo rf* cn co l ι ^ cπ cπ co rf* cn ιf* cπ cπ ιt* rf* ιo co oo t l l C71 CO LD O tO CO Cπ I LD 1 00 t I Cπ pι o cn rf* co cπ t DoO tto cOoO oO pP* oO ttOo tCo uCnι LDD ^-J tto tIo tto tto oO μP* tl>o iLDD σCnι ti0o t1SoJ tto μ μ ι σι m ui ιt* o tO it* rf* 1 CO LO 1 pi Lπ Lπ CO OO LD rf* rf* J LO ιf* l ! OO Lπ I tO rf* co rf* cn o uι . ιf.^ c -o c „o t_o_ c_n. b_θ- θ-o o -J θ _ _i L _o ρ . i L _D θ _ L _π. ι tιoo ccnn ccoo oo o cπ co o to co LD cn rf* -o o LD 00 Lπ 00 LO tO LD OO Lπ rf* pι tO O p i LO l l p t O I OO CO CO -j cn cπ to co to o -j cn o to co co cn o co cπ to to co μ- o o co oo to -J o o ~o cn LO rf* cn io LD co rf* ιf^ ιl* rf* ιf^ cπ cπ Lo cπ cπ cπ cπ cπ cπ cπ cπ cπ cπ cπ cπ cπ cπ cn cn cπ cπ cπ cπ cπ cπ cπ Lπ cπ cπ ιt* co cn cn cn ι ι n co cπ ιf* rf* c ! OT ι cπ ! ι --j ! Lo o o o u3 ∞ ι ι c cπ cπ i^ ιi* ιt* LD LD o ρ w ιl* cπ cn ! θ ιt ιl ι ∞ ∞ LΩ ιf o cn o cπ to o -J ∞ oo LD l cn Lo cπ ω LD θθ LJi Lo ] ( η rf* cn t^ pi CO LD 00 00 rf* l CO LD Cn rf* μ ^o ιt* o ω ^ lD ι^ ιt* lΛ 03 ) ^ lD ffi ω o o l lfl ω ι^ μ ιo ^ ω [D m μ o ^ ιl- ^ o) lD (!l o o ω ul ι lD _ ( _Jl . to o μ p P* oO ^LDD O tO l tO O l CO Cπ θJ O tO -j μ ^ <B θi o to i i i ιi- co μ i ()o uι μ co θ3 Cθ VD io to -j (* σι to ιo o uι ω μ uι D o μ p Cπ t 00 Cπ 1 0J LD t0 rf* LD Cn p O L0 Lπ VD Cπ 00 0J C0 O LD 0J O I I I I p i p i I I I 1 I I I I I I I I I I I I I I I I I 1 I I I I I I I I I I I I I I I I I I O tO H H O cπ n o o LO 00 ι ] cn cn cn cn cn cn rf* rf* co pι oo ιo to pι p ι p ι o o ρ ι co ιo o j to oj oo to to to oo to rf* oo oo o j to p o o o rf* rf* oo to p p ι o pi t ω iιJ U to o iD *l to uι ^ to ra ιt* to ιo ifl tJ co θ (Ji to iD θ Ui uι uι ιo uι uι uι iD ω ιfi αι oι ** μ co ι co to o co Lo ( t* co cn ρ ι cπ p ι p ι cπ rf* oJ cπ co LD LD ι to cn co o o cπ rf* co ui ιt* o co ι θ J c _π. o —o ρ . ι θ _ 'L-π c~n —rf* -J π .Lπ.. .pi. .LD.. _to-. , pι, „to, ,„3 ,θ.J, ,ι ,cπ-, ,c„π .ιιf*. ,Lθ „ .LD„ .θ— ■ .l o , - , cπ oo Lo oj ι to cn cπ pι cπ cπ co crι _ι tO -J OJ O p ι l C rf* LD Cπ ~o cn ιo cn Lo co cn μ- σ to oo p* cn to tθ rf* tf* pi rf* cπ o ι o o oo oo LD LD ιt* oJ isJ o i Lo cπ cπ ιt* ι cn cπ co co cπ to oo p* ιf* υo ρ i rf* cn oo oo cn cn -J -J pι p ι o μ μ μ μ μ p μ μ μμ μμ μ μ μ μ μ μ μ μ μ μ μ μ μ μ p μ μ μ μ μ p μ μ pp μ μ μ μ μ p p μ μ μ μ μ μ μ μ μ μ μ μ μ μ 0000000000000000000000000000000000000000000000000000000000000 00 000 00000 00 000 00000 0 0 000 0 0 00000000 0000000 00000 000 00 00000 000 0 oo cjι cn cn cπ cn cn ι ι ^ ι cn cn cn cn cπ on OT cn cπ ιf* ιi* cπ cπ ιi* rf oJ ι{* ω LD t ro oJ to LD ι ω ι-- l cπ ιo -J cπ ω to cπ o p ι o ω rf* ! to ! cπ cπ cn o --o oo p i ιf* ω cn to cπ ι o^ l t OO Cn rf* ) LD LD Ln O w co oι ιo ι ^ to u) ω uι o o uι uι iD to ω uι μ σι ii o to tΛ ι ^ μ uι to ^ μ m ι i ω m t B) ιt* to u (jι o ifl to ω σι θ vi to to LO tO Cn LO lt* lt* CO LO l l- m ω oa ui co o o ifi ω u tn μ m μ -j o μ w μ μ ui ra ^ iD to ui ^ io m i ii- o oi oj co oi ω iJ ui j iD μ ui o oo i oi iD iD σi cn ι oo cn cn ∞ cn oo oo cn σ^ cn ι ∞ cn cn σ cn cn ι co cn c^ cn cn cn ^ ∞ cn cn cn ; ; ' ; &*' iϊ' ; >' ! ; i > > > > > > < l , iϊ* , !6' ' , l> ' 33333333333333333333333 > > > > > > > 33 _ 3 _ 3 3 3 33 3 3 3 3 3 3 3 3 3 3 3 33 333 33 Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω S O Ω Ω Ω Ω Ω Ω 13 !3131313 i3 l31313 g l3 !31313131313 !31313131313 131313 g 13 !3 1313 131313 CΛ cn σι cn cn cπ cπ cπ cπ cπ cπ cπ cπ cπ cπ rf* ιi^ rf* ιt*. rf* ιf* rf* ιf* rf* ιi* co co co oo oo ω ll* C0 ISJ pι O LD 00 l cn Cπ ιl* C0 t0 H O L0 CD I Cn Lπ ιf** 0J t0 R O L0 00 l Cn LJl lt* L0 1SJ pι O L0 ∞ Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω to to M w to to to ι>o o to to to !o to ιsj to to to to to ιo to to tθ !o to to to !o LΛ LJi υι Lπ Ll Lπ Cπ Lπ Lπ rf* lt* ιf* ιt* lf* ιl* ιt* rf* ιl* ιf* ιt* rf* ιt* ιt* lf* ιf* ιf* ιl-. ιf* ιt^ o o o o o o o o o 3 LD LD 3 i-3 U3 LD ∞ co ∞ α3 θo co o3 Co oo -j ι o ι ι --o ι c cn cn cn cn σι cn Lπ LJi Lπ c^ I I I I I I I I I I I I I I I I I I I I I I I I I I I I p* oo to to to tso to ι ι cn cπ rf* to pι o o p o o o o ~j oo rf* ι ι θJ cn rf* to co oo cn rf* o ρι oo cπ cπ ~o cπ cπ ρ t [0 ~v0i c0o0 oO Cuπι uCπι rf«** Cω0 Cw0 oO tt |μ-' N il oO 0ω0 iI LιoD oO iLDD tuO tω 0[D0 oθ LωSJ ~0i twO *J σι μ ιo j . oo Lo rf* o rf* cπ pι cπ to cn cn co cπ co co Lπ Lπ tt* to Lo o ρ p p i i Lcoo cLπn ccoo ooJJ tt>oo ccnn ρi rfil-*' LLDD θO ccnn --JJ CCπn ccnn oo oo ccπn cooo oo oo ccπn rf* ( -n c j rf* o -o o co o to cn 03 LD O o l oo co oo oo o ιo l t rf* to to o oo cπ rf* cn to co ιo oo ιo ι i cπn iωo p cmπ oωo Liθn mco mcn cmo icnπ mco omo LCoi cmn cmn ciππ i Lmo ωoJ rf-*i tMo pι Ui μ αi CD f. j to μ iD μ μ o μ μ ω p to ιi_. ιl* ιti cπ ιi^ cπ cπ cπ cπ cπ Li ιt-. ιl*. ιl** Lπ Ljι cπ cπ cπ cπ LJi Lπ cπ cπ cπ w co ω co o a o μ μ μ μ μ oo ιo ιo o μ ω ω m ιt* ω ^ ^ ^ ω ^ ω ^ ij ^ ω u μ μ «) ^ ι ιo ιo o o o ιo μ μ ω to nι ι m ij (* ω to (o to μ μ o o3 (iι iD I I I I p i O O O O p i P-* O l l C0 L0 Cn l -J O L0 I L0 L0 00 L0 LD 00 t0 C0 rf* Cn C0 Cn O LD μ μ ιo σι oo ιo to μ iD U) ^ι ω ( o μ ιf* μ to ιo μ M to uι ω isJ o ω ifl iD ιi- ω ui ιt* tθ ιt* oo m LD O Lxi co rf* to cn L7i cn -j co co LD OO LD cn cn rf* cn ^i ! O to Lo oj i o cn o i cn cn oo co -o on cn LD to co o i Ln Lo p i p i rf* co Lo o o Lo -J cn oj oj (j-i pi i oJ co rf* oj oj oj co rf* oj to oj rf* cn to cn co co co o ~o o OJ OO Ln cn O p i O OJ p i LD O lf* p p i LD Cn LD O lt* OO Ul rf* LD LJl tO OJ cn if* LO O P* <-rv if* O it* Cn p i O μ μ μ μ p μ μ μ μ μ μ μ μ μ μ μ μ μ μ μμ p μ μ p μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μμμ μ μ μ p p p μ μ μ μ OOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO OO OOO OO OOO OO OOOOOOOOOO OOO OO OOOOO OOOOOOOOOOOOOOO OOO OOOOO OO OOO O to bo to to to to to to to t t>o t ω oj to ιo M to cn cn cπ cπ rf* o to w w oJ w ω cn cn cπ ιt* cn o Lo to ιt* cn oo Lo to c7i ι ι i it* ι co cπ μι oo cn -J oo -θ LD to cπ oo LD σ Lθ ιi-. ω ω t LO 00 p i cn p i O o lJ ιo μ ιB -J Ol ^ o μ ιfi ^o ιD ^ lD o ^ o μ ι* o μ oo to n3 0l ^! ιt* ∞ lD l lD D3 [o o ιo ιD l o lIl lo ι]l ω μ ^o αl o μ to <Jl O ^ ^ it* cn rf* to to o μ vj to u ιo o aι *J Ui uι iΛ σι o o w w m u ω m ω m ω m iJ tθ ιi- ω ra o iD ^ to ^ ιn ffl i (J\ σι σι σι io o w to ω ui p μ o aj oι to σι σ^ m cn cn cΛ cn cn ι co σi cn co cn cn o co cn ι σ^ cn o-ι cn cn ι co cn OT cn cn cn o co c^ > ' ϊ> ; ι t ;> ;ιs ; ;> i ; ; i» ! 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω O Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω μ μ μ μ μμp pμ μ μ μ μ μ μ μ μ μμ μ p p p μ o o o o o o o o o o O oO oO O O O O O O O O O O O O O O LD L0 ω LD L0 tø LD L0 LD L0 L0 LD L0 l > LD LD LD LD LD LD LD L0 ω L0 LD LD LD LD l-0 L0 L0 ω LD L0 LD to to to to to t μ p μ μ μ H μ μ o o o o o o o o o o iD io io io io io io io iii io oj oj iii ffl αi oj oi oj oj o j Ni ^ i i ^i j i vj Nj oi oi oi m oi cn rf* co to μ o Lo oo ι c cnn ccππ rfrf** oooJ ttoθ μ oθ LLΩD θθoo ι cn cπ ιi* oo to ι--* o LD θo ι cn cπ ι|;- θJ to o ω oo ι cn cπ ιt* co ιo H θ Lo oo l ≥i Ω Ω Ω ≥i Ω Ω C Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω 1 , >' a a a a a a a 3 3 3 3 3 3 3 Ω Ω Ω Ω Ω Ω Ω Ω Ω B B B B B B B B B B TJ TJ B a a a κ; κi κ; ι- ι<; ι<i κi κ; ι<! ι- κi co co co ω co co co co B B a B B a a B B B B B B B a a a a a a a H H H H H H H H H H B B a i κ| K! co ω co co co ω co co co co co co i Tj a a τj d a a a a a a a a a pd a a a a a a a a a a a a a co co co co co co co co co co a a Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω to t>o to to to κo to to to Ui cπ cπ cπ cπ cn cπ cπ cπ cn CΠ i_o oo oo oo oo -o i -o J μ μ μ μ o O I I I I I I I I I I I I ^ ■o oι ui ιt* μ to ιi * *. (o iΛi ιt* ιt * μ M ω to uι to μ o o μ μ μ ω to ιt* ui ιt* ht* to μ o o o μ o o o μ μ ω uι ui ιt * ω to μ o t p i o to o o o o p p i cn cn cn cn cn cn cn cn cn cn cn Ln cn cn cn cn cn cn cn cn cn it* it* cn cn cn cn cn cn cn cn cn cn c^ O LΩ LO O VO t μ μ o O LO C mn ι o μ p ω iJ tso μ μ u) a o P t ω u m μ> ui ιt* ω ^ ^ αi Ln ι ι ^ σι oι m ui ιi* ω ω cn to co o ι μ rf cπ cπ μ ι oj co -o co ] μ to ι cn Lo o o oo to cn co to cπ μ cπ cπ t rf* cn μ μ -o: cn cπ co rf* rf* μ μ μ μ-* -j to It* tO p* O rf* -J p i O OO μ cn oo o t>o μ ! ιf-. cn co cn co to M cn co i cn o j rf* i i Lo cn -J o oo cn -J o rf* cn o vi o ι ra ι cn Lo rf* to i iP* o j rf* it* oo to rf* i- i cn o to cn ιt* pi ( ι θJ o oo co l θ pι cn LD LJi Lπ tθ rf* ιf* cn l ιt* cn co oo Lπ p μ μ μ μ μ μ μ μ μ μ p μ μ μ p μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ p μ μ μ μ μ μ μ μ μ μ μ μ p p μ μ μ ~j Lo oo oo Lo LD μ-> o o o to to co to to rf* rf* cn cn Lθ co Lo co cn cn ι cn cn co rf* cn m cn i cn cn rf* it* cn it* cn cn rf* cn rf* co oj rf* to rfi. OJ Oo i co io io o μ μ to μ LD -J l μ θ Lπ θ 0J O C0 μ LΩ L0 Cn C0 t0 L0 L0 C0 LΩ lf* O rf 00 ll* 00 -J C71 C0 Cπ tθ μ Cπ ιt* t l t0 l Cπ cn t0 O M ω ι σι rf* cn Co o cπ cπ cn ρι pι l to cπ cn to co σ o cπ LD M t>o p i |i* cπ co oJ oo LD θo co LJi Lo cn ιt^ Lo oJ LD Cn to ^ ii*. co rf* co pi LD CJ to oJ cn i cn it* O i | i-o cn oo oJ cn cn to co oo o oo to oo cn LD Oo io c^ μμμμ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o tO tO tO tO IO OJ tO CO tO tO tO I>O tO t tO tO IO OJ M ιf* CO OO IO IO tO tO IO OJ tO tO tO tO tO tO IO oo ι ^ LD Co oo cπ cπ rf* co ιf_. uι ι cπ oo oj θJ o cn Lθ LD oo ^ oo cn LD Lo μ cn oo cn LJi L ι cn cn Lo ι^ o cπ rf^ ∞ to ∞ ω μ ι μ o ι μ μ ω μ ιi . cn Lo cπ μ cπ ιt* ∞ i Lo oo o LD M ιt* to cn cπ cD θJ oo ιo o Lo μ to to c^ o t to o cn LD o^ o o oo ω ιo to tIl ^) αl ^o m J μ o ω o3 ^ ω o -l tn ^ l ιt* μ to m ) ^ to (» ol lΛ ω l lD ^ M ^o ω p μ o ω l m lΛ ιt* o ^) m ul LJ 1 t LO o o 00 ι oo cn cn ι LΛ LΛ to o 4^ 4-* LO LO to LΛ © LΛ O LΛ © LΛ o LΛ LΛ ;> ; ; ;ιy ; ; ; ;> ; i ; ι> ; 3333333333333333333333333333333333333333333333333333333333333 Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω μ μμμμμμμμ μμμμ μμ μμμμμμ μ μμμμμμ μμ μμμμμμμμ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ i— , μ μ μ μ μ μ ι-' μ μ j ι-> μ-- i—' i- 1 P- μ μ μ μ μ ooooo oo ooo oo ooo ooooooooooooooo oo oooooooo ooooo oo oo o ooooo oooooo m m oo ffl ts tB ffl ^ -J -J ^ -J O i o -j -j oi w σi m m m m oi σi oi w ui ui ui lii w iii ui w cn cπ ιt* co tso μ o Lo oo ι cn αi ιf^ θJ to μ o Lo oo ι cn cπ ιf-. oj ι μ o i-o co ι cn cn rf* θJ to μ o Lo oo ι cn L^ a Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω ≥i Ω Ω Ω Ω ≥i Ω Ω Ω Ω Ω Ω ≥i Ω Ω Ω Ω Ω Ω Ω ≥i Ω Ω Ω Ω Ω Ω ≥! Ω Ω Ω Ω Ω ≥i Ω Ω Ω Ω Ω Ω Ω B > Ω Ω B > a a Ω B > B i Ω Ω B i d B Ω B Ω Ω B > Ω B > to μ to μ to μ to μ to μ to μ co 3 3 3 3 3 3 3 3 3 3 3 3 3 3 a t , a a a a a a ' ' ^ 3 3 3 3 3 3 3 a a a a a a a a 3 3 3 3 3 3 3 ω ω co co co co co co co co co B B a a a a a B B a B a a B B H B a a a a a B a a B a ffi a a B B B B B a B a a B B B a B a a a a a B a a B B B B B a a a a a jd a a a a a a a a α α a :j=' ; ' !> a a a a a a a a a a a a a a a a d jd a a a a a Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω to to cn cn oo ! co oo ω μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ t o μ to ιf*. oo oo ιf* t oJ cπ cn cπ ιt* cπ co rf* cπ ι cn cn cπ cn rf* cn ι ~J θo cn co O O LO LD O OO LD μ O O LD o μ oJ to cπ θJ ιt* oo to oJ to to to μ o Lo o μ Lθ LD o cn co Lπ ι LD Lo μ oo ιt* rf* o co μ cπ cn cn σ. cn cn rf* cπ cπ co co oo Lo i ιt* μ μ co oj ι o ιθ -i _ M μ p iΛ io o p μ μ i -j ω i ra I I I μ co _o _o oo to co co cπ rf* ι cn cπ rf* co to to μ o o μ μ μ o o o o μ oo oj cπ co rf* rf* oo oj oj μ to to oJ θJ rf* oJ ιt* cn cπ (_π (_π cn cn ι θ LD θo co σ. co ! ι co Lθ - co μ μ ι_π cπ ! o cπ to o co co cπ cπ cπ μ o o _o to μ co Lo cπ co -j cπ μ cπ tθ ιt * o ι ι cn ιo oo ι μ ιi* cπ _o ιi_. co ι_n cn cn co oo oj LD ι cn LO LO o cn co to o o cπ t Lo ∞ o ι_o co i ιt* L-i oo o ιl* cn rf* ιt* LD cn i Lι7πi μμ ιtto rfιrf_**_ cmcoo Lccooo ^ιι ooo oαo oooooo oooooo ooo ccππ cmcnn LLLoθo cccπππ μμ LLDo θo JJ ttθθ ιιti** c»o μμ --JJ c_nπ o_oo c_nn ~Ji rfιf** lι ccoo ccoo α^ μ ι σi ιi_ ιi-. ι _n co cπ co ι cn LD θJ μ _o o cn μ -θ - co LD cn cπ to o 0 - ω - -J ω lt* ^) - -o ul lo ^ tJ l ιo -l ιϊ m -- -J co * * lD μ ι ιD - ιo μ ^) -l σι ^ ω co l -o σl μμμμμμμμμμμμ μμμ μ μ μμ μ μ μ μ μ μ μ μμμ μ μμμμ μμμ μ μ μ μμ μμμ μ μ μμμ μμ μμμ ooooooo oooo o ooooo o oo oo oooooooooooooooooooooooooooo oo ooo oo ooo o ooooo oo ooo ooo oo ooooo oo oooooooo ooooo oooooooooooo ooo ooooooo oooo t tO tO M t _ M tO ! _ t tO M IO t _0 _ tO C I tO t tO tO CO _O t _O M t C IO -O W -j ι_o ι ] oo ∞ σι cπ ∞ _o oJ cn ω to cπ σ. ω ∞ ω Lo rø Lπ cn i LΩ ι CO cn ∞ σι ! θ Lπ cn o i LD Cπ to to cθ il* i Lo o o to σi -J m ui -J ω μ co t oo oo co -o ι αo μ LD ι o- ιi* μ LD θ Lπ _o rf* rf* oo o oJ co ιf_. o o ι cπ μ cπ cj rf* cn t LD co rf* μ μ co * * μ -i μ -o to uι μ ι co io σi Lo co Lo oj μ μ rf* LO oo cn μ μ o t rf* ι to LD μ Lo o oo oo μ oo o o μ LD θo i LD ι _ι ι oo μ Lo μ LD -J Ln rf* rf* ] co cn cn oo cn cn ι Jo cn cn cD _n cn ι co on cn ON LΛ LΛ 4-* 4-- LO LO t to © LΛ © LΛ © LΛ © LΛ © LΛ LΛ μμ μμμ μμ μ μμμ μ μμμ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μμμμμ μμ μ μμμμ μμμ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ o o o o o o o o o o o o o rf_> l f_. l f_. rf* rf* rf* l t* l t* CO OJ OJ OJ OJ OJ OJ μ μ μ μ μ μ μ μ μ μ O O o o o o O o o O LD LO LO LO LD LD LD LD LD LO CO CO CO ~o cn cπ r f * co to μ o LD oo ι cn cπ rf* oo LO CO cn LΠ rf* CO to μ o LD oo I cn cπ * * OJ to μ o o CO o n Lπ rf * OJ t μ o LD oo -o Ω a rø Ω Ω Ω Ω S Ω Ω Ω Ω Ω Ω Ω ≥i Ω Ω ≥i Ω Ω Ω Ω ≥i Ω Ω ≥i Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω D Ω B α o Ω B i O B Ω B ; α a Ω a Ω Ω a Ω N a Ω Ω Ω Ω Ω a Ω Ω Ω Ω Ω Ω a Ω Ω Ω Ω Ω to μ to μ to μ a > B a Ω a > a Ω B t a > Ω Ω td μ t μ > Ω B > τ_ Ω K i K i Ω ΩKi K i Ω K j B aB B aB aB B aB B ω ' ij>' ' ι i ' i >' ' t Ol -l -i tO -l B -l tO -l -l - ) Ω Ω to co co co co to a a a a a α a a ≥! a a ≥i a a ≥i a a a a ≥i Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω -0 _ t t _ t tO _ t M _O W tO _ KJ _O tO t tO _ tO tO tO -O t tO tO t t t tO M -o ι ι ] ι ι ι ι ι ] ] ι ι ι ι ι ι -o ι ι ] ι ι ι ! ι -o ] -o ι ι ! ι ι o ι ι ! ι ι ι ι -j ι ι ι ι ι ι σ^ cn n σ^ cn (_^ cπ cπ cπ cπ cπ cπ ιt* ιt* ιt* ιt* ιl* rf* ιi** ιt* oo co co oJ θJ θJ Co co t to to to to to to M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I f I t 1 1 1 1 1 1 1 1 ! / I I I t co o μ it* co to co to rf* to oo _o oo ιf_. σ. Lπ -o Lθ θo i L-i (_π cπ cπ rf* -θ θJ ιf_. |j_. oJ Cπ ιt-. o μ o to to oo to LO to o μ o o μ O t oμooo oμo oμo μ oo o rf* cn cn Cn o co Ln o cn 00 O _0 O -J μ cn cn cn cn cn cn -n cn cn cn cn cn cn cn cn cn cn cn cn o. ai cn -n OT cn cn cn OT oo Lo μ μ μ o o o ∞ ι_o ι co co co LD θ LD o t μ μ to to ιo o to oj μ _o μ to θJ Lπ Lji Lo LD oo oo cn cn Li LJi Ljπ LΛ co ~o μ ιf* tθ ιt* co _o to tθ ιt* o cπ ι μ cπ ι cn to rf* Lo μ o l i-o μ cn co rf* -o oo Lo cπ to μ o cπ to ι to σi Lri L_ ιt-. co cn o co Lo co --j o cn cn co cπ ιt* cj cn cπ μ OJ rf* ι cπ oo oo co cn μ μ LD Ljπ t cD co o tθ L-i ιf* cπ rf* _o -j μ co μ oj rf* crι cπ cπ ιt* cπ μ co μ cπ co σι ιt* cn ι oo o o cn ιt* tθ rf* rf* cn to o cn rf* co co ιf_. co co rf* ω o p μ co μ to o M co to co Lo cn LD cn cn LD rf* Lθ to oo oo co _o LD ι cn ι ι - oJ cn ιt* ιt* o cπ μ co Lo cn o ιf* -o _o co to ιt* ι cn l to to μ (jι rf* o cπ ι cπ cn co μ μ μ μ I I i l -O tO CO LO O O lO OO OO O it* to oo (_n cn cn cn cn co i -o cn cn rf* OJ oo O o o μ to _ - _o μ o o μ μ oj oo cπ cπ _o ι ι cn cn cπ cπ rf* cπ co rf* rf* oj to to o o t-' to o i-o i-θ co Lo oo Lπ μ ι o oo Lo t ιf^ μ Lπ to cn ιt* rf* μ o co cπ ι o L7i _o Lπ cπ co μ cn ιl* cπ co -j ι o ιl* _o co cπ μ ι co rf* ι ι to cπ o co o co _n ι o ιf* rf* co i Lo ! μ oj o L-i ι to co μ ι co ι co μ co cπ co cD ∞ σ. cπ LD to cn LD cn LD _o _o co rf* co co ι oo cπ to LO -O LD OO OJ CΠ CΠ O -0 CO o t_ to o ιi- to μ to o *. u: o co σι iD _ι tθ Nj ιo vi oι μ ttJ Ui ι- _ι ω -i (* o o (iJ - μ μ μ μ μ μμ μμμ μμμμ μμμμμμμ μμμμμμμ μμμμμ μμ μμμμ μμμ μ μμμμμμ μμμ μ μμ μ μ μ ooooo ooooo ooooooo ooo oo oooooooo ooooooo ooo oo ooo oooooooσoooooooo ooooooo ooo ooooooo ooo oo ooo oo ooo oo oooooooo oo ooo ooooo ooooo oo ooo o ιf* ιf ιt*corf* ιt*ooωωoorf*ω ooωωoorf rf* ( _πcn σ^cπ ιt*ιt*ι!**ιl cn cn^ cnω o i!o μcn ιi Locπ t L-. cnω ! ω_θ ιt * ooμ σ.t !t _oM toιo oLooωcπ !σ.cn i o o to rf* ∞ Lo oo μ o cn cn μ co o o ιo rf* ιi^ cπ cn μ cπ -θ co co co oo ι cn ιi^ o to oo μ μ i (_^ cn μ θJ co μ Lo ι ω LD o ι_o tθ ιi-. LD Co μ ι oJ co LD ι cπ cn l cπ σι ! iD θ ii* oo - Co cn cπ o ιl* μ μ ι_o ι oo co o co μ j o to ι_o _^ μ cn cn ∞ cn cn ] co cn cn cn cn cn cn -j _o cn ] co cn σ. cn ^ co cn ^ » -n cn cn ! -o cn ] cn -n σι σ. LΛ LΛ 4-* 4-* LO LO to to © LΛ O LΛ O LΛ © LΛ o LΛ LΛ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ to to to to to to to to -o μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ O O O O O O O O O LO VO LD IO V- VO VO VO IQ LD OO CO CO CO CO CO CO CO CO CO -J -J -O -J ^ ^l ^ -O -J -J OΛ -^ co ι cn Lπ rf* co ι.o μ o LD oo -J cn Lπ ιt* J to μ o ι > co ι cn LJi ιl^ co ιo μ o Lo co ι cn Lπ ιti ω Ω Ω Ω a Ω Ω a Ω Ω Ω Ω 3 Ω Ω Ω Ω Ω a Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω ≥i Ω Ω ≥i Ω Ω Ω Ω ≥i Ω Ω Ω a Ω Ω Ω Ω Ω Ω Ω Ω ≥i Ω Ω Ω Ω Ω Ω B d B Ω B i Ω a Ώ Ω a Ω Ώ Ω B to μ a > > > Ω B Ω B i μ μ to a μ μ to a ι> a to a μ a a a a ! i i i ' l i co co co co co co H 3 H H H H H H H H H H H H t B B M B to ra to co ω ω ω co M B B M H a a a a a a a tA f tr< tr< tr< f a a H , a to w to ra to to co co aaaaaaaaaaa a a aaBaHaBB Ba aaBBa B ≥i a a a a ≥i a a ΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩ tO t>O M tO t t tO tO tO tO tO ! t tO t tO -O tO M [ M tO t tO _ tO tO I !O IO co co co co co co oo oo oo αo oo oo oo oo oo oo oo oo co oo co oo ∞ co oo co co co oo co co oo co co co oo oo oo oo ∞ Lπ cπ cπ cπ ιf^ ιt* ιf* ι(* ιf* ιf* ιl* ιf* co θJ θo co oj _o _o _o ^ _o w to t o μ μ μ μ μ μ μ μ o o o o o o o o I l I I I I I I I I I I I I I I I I I I cπ cπ uι cπ cn -n rf* ι cπ crι cπ rf* cn rf* co oj oo to oj to o o o o μ o to μ o o o o o o to μ μ to μ o o o o o to co to co rf* cn cπ ~o i cn cn co rf* _o oj rf* o o ιt * ιi* ι o ιo o iD J iD ι o ιo w M σι i_ oι oi -i ι» _ι μ ιo - - μ co i!J uι to ιt* ι o oo to o cn cn cπ μ co cπ LD Lπ o ι cn υι μ cπ to oo ι*o -j co oo cn cπ μ _π rf* L-i ι o θ Lo μ oJ θj μ !θ to _o rf* o o o σι θJ cn o oJ co -o cn rf* co Lo μ Lπ to oJ LD to oo oo LD Lπ o co to o o to i* μ Ni o. o o ιθ -) iD ι. iD u ω o _) i ι oι cn i μ j μ -i iD iD vi ιi* -j μ o μ μ oo Lπ Lo cn rf* μ co cπ cn oo μ ιi* o ι t oo _o co μ ι σ^ σ 3^ cn cn o^ -n cn σ^ cn c o^ cn cn c (_^ σ^ 3^ α-ι ^ σ σ^ σ^ c 3^ c-^ c-^ o^ c c c^ cπ cn ιt* cπ co rf* rf* cπ rf* rf* cπ cn ι ι -J CD _o -j ι ι on i LD CD cn cπ ιf* rf* o μ M -θ CJ Co rf^ ι o >_ ιf* l i ιt* μ Lι o to μ LD ιt* ιi-> cπ t ιt* cn co o μ μ μ ! co ιo σι μ co o o to oj Lo _o co cn ιt* ιf* cπ co μ ι i Lo cn co LD cn co μ o μ ιi* μ μ μ μ μ μ to to μ μ μ μ μ μ to μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ to to to to to μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ to co cπ cπ cn o o o co o ~J co co o _> co o cπ cn co ιi* ιi- * cπ cπ σ. cπ cn σι C ιt* Cπ cn _o oo LD θ - μ μ o ω ω io o- co o i s] cn O -n Ln (_n cn rf* co co oo rf* oo oo _o o ι cn cπ to _o o -o. _ μ cn cn μ oo LD i rf* Lπ co o o cπ co Lo ι _o to ι_ (jι o ω o ι Loo ιιtt** --jj μμ to oo ι o o cn ι o cπ oj μ oo oo bo rf* o oj oo ι -J o lD ω μ ra ιl- o. o -o cn o co co Lo μ -j co ! rf* o oj oo _n ιt* μ o μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μμ μμμμooooooooo μμμ μ μ μμ μμμ ooooo oo ooo oo ooooo ooooooooooooo oo ooooo o oo oo o o o o o ooooo ooooooooo ooooo oo ooo ooooooo oo ooooooooooo oo ooooo ooo oo oo o oo ooooo ooo oooooo -O to to t to to co oj oJ to txj to co c cπ oj to co t to co co co to to to cJ co oj co cπ oj oJ OJ i^ l σθ ll-. Cπ -J t LD ιt* Cn LD l Cn θ O _0 l _θ μ l l l_0 l CO LD LD l_ l l LO rf* L_ (_η LD 1 0J ω cn oo to cπ o o l cπ oo to ιt* ιo oJ to cn ιl- * ιi* cn ιt* ι o to to μ μ cn co co ι-- o cn ι oj μ cn to to cπ UJ ι to i Lo ι o _o ι cn to to _n ι co tø μ oo _o rf* σι Co oJ o cπ to ι to co μ Lπ oo ι oo cn ι co oo σι cn σ^ cn -J oo o^ ^ co σ^ σ\ σ. ] -θ cn cD cn cn ι oo cn σ^ cn cn cn cn ι co cn cn cn cn cn cn ι oo σι ι co o to o LΛ LΛ ^ 4-- LO LO t LΛ O LΛ O LΛ O LΛ © LΛ LΛ Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω O Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω O Ω O Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω I313 !31313 !3 !313 I3131313 !3 S !31313 I3 !313 !3 !313 !313 !3 !313 I31313 S μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ to t to to to t to to to to to to to t to to to to to to to to io to to to to to to M to t to w cn cn cn cn cn cn cn cn cn σι cπ cπ cπ cπ cπ cπ cπ cπ cπ cπ ιi* ιf^ ιi*. ιt* ιt* ιl* rf* ιt^ iD θo f _n ιt* co to μ o LD Co ι cn Lπ ιt* oo to μ o LD _o i OT Cπ ιf-. oo to μ o i-θ Cθ l cn cπ ιt* co ιo μ o L^ Ω Ω Ω ≥i Ω Ω ≥i Ω ≥i Ω Ω Ω Ω ≥i ω Ω Ω Ω Ω ≥: Ω Ω Ω Ω Ω Ω Ω ≥i Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω ≥i Ω Ω Ω Ω Ω ≥i Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω ≥i Ω Ω Ω Ω Ω B i B M α d Ω B i Ω B > a a Ώ B : tsi B B a a Ω B t a Ω μ a : a B to μ a Ω a a a to μ μ to o to μ to μ to μ Ω Ω Ω Ω B B B B B B B B B B Ω Ω Ω Ω Ω Ω >' ' Ω Ω Ω Ω Ω Ω Ω Ω Ω a a a a a a a a a τ. τj a a τj a a a τj Tj TJ a a a a H μ H H H μ H H H κi κi κ! κ! κ! κ; to co to ω co ω ω ω a a a a a a a a -*S f-j r^ H-_j ("^ l" Kj Kj a a a a a a a a a a co co co co co co co co co co co co co co co τ a τj a a a τ_ a a a a α a a a a m tO C tO tO tO ϋ- tfl t B H a M B B B B B B B a Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω to to to to to to to to t t to to M to to to to to to to to to to to to tO M to to M to to t^ LD LD LD LO U- LD LD lJ0 LD l_0 LO '-0 LD LD LO LD LD LO Lθ LD 00 00 CD CD CO 00 C0 C0 00 00 00 00 00 00 00 00 00 00 00 ∞ to o to to μ μ μ μ μ μ μ μ μ μ o o o o o o i-o LD LD LD Lo u- LD LD θo oo co oo oo co oo co oo ι ι ι ι ι l -o ι ι cn cn σ^ -n cn cn μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ to μ μ μ to μ μ co oo μ to to μ o cπ cn co LD ι ι oo co μ μ L0 C0 LΩ ] _0 ! ] 1 00 _0 LD L0 μ θ <-3 SO O LD O μ o ι 00 -J LD O0 LD LO LD i Cn Cn ιt* rf * o ι oo cn to ι _o o rf* co co Lπ rf* μ _o co LD θ ι oo oo Lπ θ L-i ι oo cπ μ to ιt* oo i LD co rf* μ ι oo co cπ t cπ o LD rf* cn μ o LD rf* Lo μ cn μ Lo _o co LD oo rf* μ o cn Lo μ oo Lπ oo ι to μ to rf* to co rf* cn co co to co co μ cn _n . μ c .n . ^ _ . t .o o _o_ t _o . cn i Lπ cO θ -n cn i LD Co co cn it* ι -J co rf* co to co cπ μ o LD θ tθ Lo μ - _o o o o Lo cπ o Lo μ oo co ^ _ cn ι μ μ θ -θ -θ LD t Lo co LD θ to cπ LD oo cπ LD ιf * μ to oJ θo cπ oo to co co Lo cri rf* _n Lrι ιo LJi co cπ ^ ι o (_π i LD cn μ μ ι to _o cπ cn cπ cπ cπ cπ cπ cn cπ cπ cπ cn cπ cπ cπ cn cπ cπ cn cπ cπ cπ ι ι cn cn rf* cπ cπ cn σ. cπ cn cn cπ ^ cn ι_n ιf* (π cn -J ι oo oo o o o Lo o o o o ιo μ μ o μ ω No co ι ι -j cn cπ rf^ ι co cπ co ιi* ι μ oj ι μ M cπ co co μ _n ι cπ ι_o i rf * LD ι μ cn Lo rf * o ιt* μ μ t Lπ ι μ cπ cn μ cn o cπ _o oo cn Lo ω -J co μ _π μ Lπ cπ co ι i* θJ LD rf * o Lπ co M CD μ . cn ι _o cn μ co _o θJ Co ! ι o co cD LD θ Lπ co cτι ω ιo σ. oo ιt* to i L-i lo α- tθ ιt * l oo μ σ <xι μ to cπ μ ιt* oJ co cπ ιt* o ιt * to ι ι cπ ιo o u3 LD Co μ ω μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ to to to μ μ μ μ μ μ to to μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ ~o cn cπ rf* oo co ι ι co co Lo μ μ μ μ μ to to _o _o rf* co to μ to to co rf* cπ cπ o μ o Lo co ι cn ι ι μ o Lo ι co cn cπ rf* ιt* co co o o o to co oj to oo o μ σ. μ (_π o -o. co rf* cπ -o ι cπ oo -o μ o cn μ μ to oo θJ ιt* oJ o oo cπ -θ LD C o l cn ι c _π. c_o_ o_ o_o_ c_n. o_ c_π. ( —-j μ . co o t cπ _o rf* ιt* _o rf* o _n iD _o μ cπ oo co cn μ c _π. _rf_* .μ c _π. c —o ,μ „oo .μ o „o _,- L_D θo μ o o rf* oo iD Lo μ cπ co ! i Lo o cπ co l to oo μ ιt* co cπ cπ co cπ ι cn -θ Cπ μ cn l to μ Lo cπ μ ι to cπ Lo ι cJ cπ _o cπ co cn cπ μ M cπ oo LD ιf_. ιi* _o cn o o _o rf* Lo cπ oo ι to cπ cπ LD to _o _o oo oo Lo cn cπ cn o ι co oo oo Lθ ιf* o μ rf* μ Lo Lπ rf* (_π cn -J cn cn ι_π o i LD θ cn Lπ Lo μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ ooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo ooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo _o to to to to to to co to to to to μ to !o ιo to to _o to t _o _ - to _o _θ - M to cn c^ co ι μ o cπ to cπ o -j σ. oj _π i_3 μ ιf* σ. ιf . co μ to ιt cπ cn cn ! cπ ιt* cn cn ιf o cπ rf* rf* cn Lo co oJ co co cπ co to co ι oo co μ μ o a M -J -, -- -J Ui -i CD μ vi -i i oι _) m ω m o -J -i -i m o -j -i -j o iJ iD vi t to ι m o (* _ι t iD -J ι 0 0 0 ~0 l rf* tO rf* LD μ Cπ VD rf* LD O OJ i iB U t ιo -o ω j _ι o σι σι m i- tJ -i -) m μ co _ι θ3 M co μ _ι i ιt* -i > o o ιt* (ϊι io ιf* θ i ij ^ Ln -J u> _ι σ^ σ. cn ] _o cn ! cn ι cn cn cn cn ] σ. σ. cD _n cn ι co cn co co cn cn cn ι x> cn co oo cn cn cn σι ^ LΛ LΛ 4-* 4-* LO LO to to © LΛ © LΛ O LΛ O LΛ © LΛ LΛ 3333333333333333333333333333333333333333333333333333333333333 ΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩ ΩΩΩΩΩΩΩΩΩΩ ΩΩΩΩΩΩ μ μ μ μ μ μ μ μ μ μ μ μ oo co co cJ Co oo oo co co c co oo oo oo oo oo oo oJ Co co co co oJ Co oJ OJ Oo oJ Co oJ OJ to to to to to to to to to M to co to to to bo to to to to to to μ μ μ μ μ μ μ μ μ μ o o o o o o o o o o LD LD LD VD LD io LD LD LD LD oo ra co co co co cD oo oo co ι ι ι ι -j ι ι -j ^ o ι_o oo ι cn Lπ ιt* co to μ o LD co ι cn cπ ιt* co t μ o Lo co ι cn cπ rf* co to μ o Lo co ι m Lπ ιi_> co to μ o L^ Ω Ω Ω S Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω S Ω Ω Ω Ω Ω Ω Ω a Ω Ω Ω Ω Ω Ω Ω S Ω Ω Ω Ω Ω Ω Ω 3 Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω 2 Ω Ω 3 Ω Ω Ω B U* N -)-) ϋ DQ B> a a Ω B a a Ω B !> Ώ Ω Ω a i a Ω Ω a Ω B Ω B > B B a to μ to μ to μ to μ ato aμ a μ a N B μ to to μ ω ω ω t__ iτj iτj τ . τj j τj τj d . ifl ι→ _^ _r ι _r ι a ' ' >' , ' ' μ H μ μ μ μ H μ a a a a a B B B B B B B B B B B B B B B B B B B B M ω ω ω ω ω ω ω co t-_ co co ϋ- i-j ω ϋ- aa aa aa a Ω Ω Ω B B B _ a_ a _ a a a a a a Ω Ω _ _ Ki Ki κl Ki κ: κ! κ: κ; κ; ι __- ι __- ι t_- _ _ a a a a a B B B B B B B B B c τ τ. τj τj iτ_ τ. τ iτ3 T τ j j i ϋ τj ϋ τ B B B a B B B B ω ω ω to t ϋ_ ϋ_ ϋ_ co a a a a a Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω lo t io w to to io to to to to to io to to to to to to t to to to to to to tJ to Ko to to to i*^ Lθ Lθ LD LD _o co co oo 3 -o co co co oo oo ι ι -o -j ι ι ι ι cn σι σ. σ^ cn m cn cn cπ cπ cπ cπ cπ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ cn it* it* co oo oo o o o μ μ to co to μ o o ] cn ι co Lo o o o o to μ o μ μ co to cπ cπ cπ ιt* oo to μ μ Lo co O O lO lD lO lD _1 01 vl -l sl (D _ μ θ -J i- U ui -i to ^ _ -j ω ω i ιo _ι w μ ω ω M ω -j ι» co uι ω o c_ ^l ιl^|-. ιrfi** ωtO C-πi ιrft** L-D C-OJ cOoO C_πi MlO O IO -O O t μ θ CO LD μ LD Ljπ θ O ll* OJ μ LD LO l l rf* LD CO OJ cπ LD _n _π Lθ ιl* _o -j μ rf* o μ ιt* Lo ι co μ o - cn μ μ o cπ _π ι ιff** cwn ttoo ttoo c_ππ oθoJ ccππ ttoo ιι oo co Lo oo > Lπ co μ _o o ιt* μ o t LD μ oo cn o μ cn o o j -o -j LD oo μ ιf* ιt* to cπ cn LJi -n μ crι co cπ _n ι o _o Lo Lo μ uι cπ rf* ι o _n rf* μ rf* LD oo μ i LJi o LD tso μ μ co o Lo co cn -θ Lθ ιt* to μ ιt* ιo o cn _n cn cn σ. Lo ! rf* co ιt* ιt* ιt* ιl^ rf* rf* rf* rf* rf* ιl* ιt* ιl* rf* rf* rf* rf* rf_. ιt* rf* ιf* ιf* rf* rf* _π rf* cπ cπ cπ cπ cπ cπ c^ LD Lo _o ι _π _n ιt* ιl* -n ιt* -n cπ cn cn ι cn ι -J σ. ι co cD o ι_o o cπ rf* ιl* co μ ιo o μ ιl* μ to ιo μ μ L^ ∞ cn μ LD ι i Lo o t Lπ ] Lo cπ _n -J ∞ -o o Lo iNθ cπ ∞ μ Lθ L-. ω ιt ω o co o ω to o LΩ Lo i -π co ι cn oJ cn cn ιt* co co ι ι co co cπ o co o rf* ι θ Lθ - o μ Lπ _o to cn μ co ιt* o ιi* Lo oo L_J to cn μ uι ι to M Ln i _n to to cn rf* lo i i_o cn cn it* l rf* co o oJ oo cn LD O tO rf* to cn i to t LD i L_ o oo co oo lo o to M to to to μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ to μ to to to to μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ o μ μ LD LD LD 00 rf* 00 Cπ j* cπ σ. _o oo Lo oo cn co ι -o _o oo o Lo o o o o Lo _o co i rf* rf* Lπ cn _n Lπ cπ to to to rf* rf* cn cn Lo co co cn cn cn Ln -j cn co i i o to μ Lo cn co Lo co U- ~J oo -J oo μ μ oo LD o ui rf* rf* oo ι ι ι μ μ μ o cπ μ t cn co ιo o co to ιi* ι i\) cn μ cπ _o ιt* co Lo o Lθ ιt* o cπ μ rf* co o μ cn co co -J O O- OO LO CΠ CΠ -J . tO L-l Cn t LD OO -O LO _π μ θ OO O rf* LO OO LO l - tO l LO _O Cn -J _ .ιl.* co o μ rf* to μ co α- LD LD rf* oo tθ LD Lπ _n co rf* to cπ ιt* o ι ι oJ cπ _n co oJ ι _n cn rf* Lo co ι μ co oo o μ μ rf* cn rf* Lo μ o - rf* oo ι _n rf* cπ o rf* LD cn - _o rf* μ co μ Lπ o to _θ Lπ oo θJ rf* u. μ co υ η o μ θJ ιf* ιf* cπ C Lo cn LD μμμμμμμμμμμμμμμμμμμμμμ μμμμμμμμ μμμμμμμμμμμμμμμμμμμμ ooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo ooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo l l-. _ _ tO 0 t tO N0 t μ -0 t tO t t _ - C0 _ _O _ tO !O t N0 C0 OJ t0 t t0 t0 tO t ιt* ω co co l cπ cn -θ _o to co LD θj μ μ to o _π _o o Lθ Lπ -θ σ. θJ Lo i LD μ LD ! Lo oo _n cj μ M l l co co oo μ _θ M _^ co o Lo ω μ to co co μ cn θ3 cn -θ Lo rf* co _n o to ι_o cπ u- - co o μ cj -j j θJ Lπ cπ t ιf* Lo co ι o -n J θ ι c^ cπ ιi-. Lo o ιt* rf* Lo Lo o μ _o Lπ o co cn cj. o oo cπ σι o t cn i-o μ cj ι μ co ι_3 _o oJ to to rf* μ _o -j ι co σ. cn ^ co cn cn σ. cn σ. _n -n _n σ. ι co cn cn cn σ. cn cn ^ oo on oo cx- cn cn cn ^ co cn oo oo cn c^ μμ μμμ μ μ μμμ μμ μμμ μμμμμμμμ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ O OOJ OLOO OOJJ OOJJ CLOO CLOO CLOO CLOO CLOO OOJJ CLOO CLOO OLOJ OOJJ OOOJ OUϋJ COOJ OOJJ OUO- OOOO OLΛJ- O_JJ OLλJ- OJ CO CO CO CO CO CO OJ ω . --. ._- , c--o. ω-_. -o-D. c-o- c-_o. -c-o. c-o- θ--3 C-_o. c.-o. -.j. .. πι i . . i. _o ■ , o_ . *i ! -Jj cmn cπ cπ cπ cπ ! t rf μ o Lo ∞ ι cn cπ ιf- co to μ o LD OT ι cn cπ t i_ oo t μ θo L-Ω _ -j μ o - _ i m uι ι* -J to μ o - m -J oι ιn t * -J to p Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω S Ω g Ω Ω Ω Ω ≥i Ω Ω a Ω Ω Ω Ω Ω ≥i Ω Ω Ω ≥; Ω Ω ϊ-i S ≤ ϊS S ≤ ≤ SS ≤ ≤ -a ≤ ≤ S ≤ ≤ ≤ ≤ ^ S ≤ ≤ Si Si ≤ Si Si Si Si ≤ ≤ ≤ ≤ S ≤ N B B a d Ω B N B tl Q tlO > 3 to μ μ to -g ≤ ≤ S -a ≤ ≤ -g sS ≤ ϊ-i sS -i -S ≤ si -S si -S si si ≤ si S. ≤ ≤ -S S S ≤ -S S S ≤ si B B B B B B B B B B B a a a a a a a a a Ω Ω Ω Ω co co S3 -3 S3 S3 S3 _g S3 3 __: s: S ≤ ≤ S S S; ≤ β S; s; s; S s; s; s: s; s3 -_; ; 3 s S; 3 S s Ω Ω Ω Ω Ω Ω OO OJ OO OJ OJ OO OO OJ OJ OO OJ OJ OJ CO OJ CO OJ CO OJ OJ OJ OO OO OJ tO tO oj oo oj oj oo oo to -o to to to to to to to to μ μ μ μ μ μ μ μ μ μ o o o o o σ o o o o o o o o o o ^ o ^o 'o-' 'o-* ^o' ^o 'o^ 'o-' 1 LD" 1 L^O cπ rf* co to μ o LD θo ι cn Lπ rf* co to μ o 3 ∞ ι cn cπ ιi* co t μ o LD θo - ι _^ cπ ιi*. co to μ to to to to ιθ M μ μ μ μ o o o o LO LO μ i μ i μ μ i μ μ μ i μ oo cn ~J LΠ σ. m ι cn cπ oo o Lo oo oj μ -J Cn LO LO Cπ ιf* IO l LO OO O Cn θ CO LO OO LD I LD o} - t θ - θ co ω μ μ μ c, M o . μ ι it** u-u) lμ- ' v —i ι cn co cn co to co μ cπ cπ to rf* cπ o uι -0 L0 l O cn it* rf* rf* cn cn rf* co cn cn _n rf* if-. o^ cn to it* ii* cn cn ii* co co _i o^ cn cn co co cn cn co co if* oj cn cri cn ω si ι_ u o. ∞ o „ ^ μ u w c, w o tθ vi M -i _ o ω - - ω ω μ ω w μ o w p -j p u _ o w ω μ ω p - _ _ ^ *] si ^ CO LD CO LO t cn LD lt- OJ μ rf* OJ ιl* O Cn CO tO Cπ LO -O CO O - LD -O OO ιl Oθ μ L-i LO O Cn Cπ rf* cα ~j cn cn co oo cn cπ μ μ rf* to o oo μ cπ cn μ to Lo co co cπ co LD LD ιt* cπ μ ιt* oo cn l θ LD Cπ cn cπ co co μ ι μ ιt* ω oo cπ to _^ cπ ιi-. o^ μ cπ o ιf*. -J ι oo co ιo to -o μ μ co to cn Lo o co ι cn cπ μ cπ o - θo co ι oo i Lo oo μ ιf_ o^ oo Lo l θ ιt* Lo t ιf* ! Lo co cn ιt* cn ω ιi cn ιt* cπ ω cn ∞ rf* ∞ t !t* rø o o M o o rf* cn μ o rf* cn o μ cπ cn LD t LD to o Lπ cπ o cπ cπ cn rf* rf* to μ μ μ co to i OJ OJ I μ to to μ co to i μ to μ co i μ μ μ μ oo to to to μ μ μ to to to to to to to to to to io M lO -O to t - -O to t to cπ to μ o o co rf* μ to to o μ l μ rf-, ||^ rf^ l|^ O l l* lf* θ μ _^ O CO ιt* O Cπ LD μ θ μ ιl^ C ιf* CO LD CD LD O θ μ t t ll-. L Cπ ιl-. CO μ co o oo oθ ιf_. oo ι cπ μ μ rf* to co o μ ιt* o co ι ! -j rf* cπ Lo to co o rf-. cn co oo co ι μ μ o - . ιf.* ffi ω „ι^ ι „f* -j -j iD o o ιt* o ω co - oι -. ιt * . oι ω -. ιi-. -o μ ~J cn co cn oo cπ o LD to ι cπ cn o cn co o mo . i i - h -, . i m ^i dj K j ^ ^ o μ ^ - dj ^ io io ^ c-πl Oo ^ι CcoO Irf-* i LD Lπ cπ LD μ Lπ cn cπ o rf* co co o μ oo o rf* oo oo to Lo cπ oo to oo -o co rf* μ o l oo LD Lθ Lo cn rf* o μ LD oo oo tθ Lo μ oo to ι μ Lπ ιt* ιt* to oo -J cπ oo ι o rf* cn μ oJ o oo rf* rf* o cn o co Lπ LD rf* oo LD cn μ μ μμμ μ μ μ μμ μ μμμμμ μμ μμμ μ μ μ μμμμ μ μ μ μ μ μ μμ μμμ μμ μ μμμμμμμ ooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo ooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo o-ι rf* rf* cπ ιt* ιi* co ιf^ cπ cn ιf* cn cn ιt* ιt* rf* ιf* cn ιf* ιt* cn rf* ιf^ cπ θJ Cπ rf* co ιf_ ι_- Cθ ιt* o^ to cπ ιt* ιt* -o μ to ι oo Lπ ιt* ιi* ι oo co μ co ^ to o LD Cπ co Lo oo co to oo Lπ co o rf . cπ to μ co oo cn cn o -θ rf* σ. cn co cn to to ιo μ ιf . co o cn o Lo oo ιo cπ co cn o co to rf cn co cπ o o ιt* ιf ιo cπ co Lo ! ι t to cn cn to co co u. ιf- cn co oo t μ co ∞ co txi -o iB ra co ra co co co o- oo -j co co co m oo co cD ω ∞ μμ μ μ μμμμμμμ μμμμ μμμμμ μμ μμμμμμμ μ μμμμμ μμμμμμμ μ μμ μ μ μμμμμ μμμ μμμμ μ μ it- * - rf*. rf- ** - rf= ^ rf- * - rf* * - rf= * it-*- rf^- rf=*- if-*- rf* * - rf* * - rf- * - rf*- rf*» rf- * - rf=- rf* ^ ιP=- rf**- rf* * - rf=- rf-*- rf= * -P*. rf*- rf* * - rf* * - rf* * - rf- * rf* * - rf=- rf= * - rf*> rf- * rf=- rf*. rf_» rf=_ rf*- rf-*- rf*- rf*- rf*- rf=- rf=- rf*- rf=- rf-*- if-*- rf-» -*. co co oo oo co co co co cπ cπ cπ rf* rf* ιt* ιl** ιt* ιf* ιf* rf* ιl* ιl** co oo oJ θJ θo oo oo co oo co to to to t to to t t M to μ o ^ oo l cn cπ ιf-* co to μ o Lo oo -J cn cπ rf* co M μ o Lo oo ι cn LJi rf* θJ to μ o LD Co ι cn υi ιl* ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω 3 _s ≤ 3 s3 3 _s _g -s; s; 3 _3* s; s; s; s s s s; s: s; s s ≤ s; s: s 3 S ≤ sS « S3 3 -≤ ≤ s ss s3 3 -S -_: -3 .i3 3 iS i? $ S fS S3 S S3 S S S3 S3 S3 S3 S S3 S3 S3 S3 S3 S3 S3 _S S3 sg S S sg S3 _S S S3 S3 i !> > > > S S ≤ ≤ ≤ ss ≤ ≤ ≤ g ≤ S S ≤ ≤ ≤ sS S i > i> itϋ i-n , i ' ij* , ! ;y ; ; ; , ! > i > 33333 3 3 3 3 3 3 3 3 3 3 3 3 3 H 3 3 - 3 - 3 - 3 ^ - ^ - ^ " 3 3 3 3 3 3 3 3 ^ ^ ^ S & ^ ^ ^ S S ^ ^ ^ & ^ ^ S S S & ^ ^ ^ ^ S ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ & ^ ^ ^ ^ ^ ^ ^ ^ ^ S ^ ^ ^ ^ S ϊ ^ ^ ^ ^ ^ LD Lo ι_- LD LD Lo i-θ co θ3 θo ∞ co oo oo oo oo cD l l ι ι ι ! l ι ι ι σ. c^ cn cn cn cn cn cn cn cn cπ cn cn ιt*. co to μ o '-o co ι cn cπ ιt* co to μ o ι-o co -J cn cπ rf* co ιo μ o LD co ι cn Lτi ιf*> co to μ o tø I I μ μ i μ μ i μ μ μ μ i μ i μ i μ μ i μ i μ μ i μ i μ μ μ μ μ i μ μ μ μ i μ i μ i i o t ι cn co -j tθ Lo LD ιf^ Lπ to μ cn i -θ t>o rf* cπ o to o υ-ι cπ ιt* co to o rf* cπ cπ rf* co μ ι o cn cn Lo oo to oo cπ cn cn o to rf* μ oj cπ un rf-. μ rf* co μ o LD OO l U- OO LO Lπ ~j μ LD μ CD θ μ θO l l tO -J cπ tθ LD to LD σ rf* cn to cn o to cπ μ LD Lo oo Lo μ * j cn to i rf* oo cn o to oo o o rf* cn -j i oo o o rf* O rf* cn cn if- * co cn OJ Cn rf* cn cn cn ιt* cπ t Lo μ ιt* oo to σ o ∞ ιi* cπ oo cn cn μ o LD Lo to θ μ L LD to oJ Cn cπ μ μ o cπ μ cπ cπ oo cπ oJ ιi* co LD -θ i to μ to co to μ μ μ i μ μ to ω μ to to to to oo to μ i to μ to to to i μ to to μ μ μ to μ μ to cπ to μ o LO o to o μ co o μ co tθ Lo cn μ o μ ιf* oo o o to ιi-. L-i μ cπ Lo rf* to to cn Lo o co rf* cn to oo rf* μ μ cn μ _- θ cn co -j Lo cπ co cn rf* o oo cn cn cn cn μ cn ι μ -o ι ccnn rfrf** ccππ ιιoo ιιtt** rfrf** oooo ιιoo ttoo ttoo μμ oooo oo ccππ ιι>θθ LLDD μμ ccnn ∞∞ σσιι CCoo ιιoo μμ ccππ LLDo tloo ccoo LLjπι cooo ιι co cπ cn μ to rø o μ oo co oo rf* oo ι cn to LD o co rf* cn to i o ooo oooo Loo oo o ωo raco ι_-o rf^* ιι oι ocnι !to μμ ιιti:** ιιtt**. * ιιtt** c-o- θi L-o oCDo L-D oθ c-ni ιιlt*-. '--5 oθ ii bMo oι ι__- ccoo ccoo c-πi θι Ln - uι ω rf* cn co rf* oπ cn μ co co -j ι co rf* cn o co oo oo ! cn ! μ σι c. -j to j* c. c. w μ o. w σ) _ - - o ∞ -) o o. - μ o ^ -j w _ w μ ui -i -. o o. ιjι o to μμ ιc_o cn l rf* μ o cπ Lo rf* Lπ to to μ μ μ μ μ μ μ μμμμμμμμμ μμμμμμμμ μμμμμμμμμ μμμμμμμμμμ μμμμμμμμμμμμ oo oooooooooo ooo oo σoo oo ooooo ooooo oooooooo oo ooo oooooo oooo oo ooσo ooooo oo ooooo oo o oo ooo oo oooooooooooooooooo ooooooooo o oo oooooo ooo l f , C Lπ rf* rf* l f , l f* l t ll . |f* l t* 0J CO CO rf C0 C0 OJ OJ OJ C0 00 CO 0J rf* OJ 0J 00 || OJ OJ OJ 00 0J OJ CJ rf* ω cπ ι o ιo cπ o cn ! ιf* ι cn co oo μ μ ιt* cn ! l Lo cn co u. co cn ιf* ι-3 cn o ιl* o oo oo rf to tθ ιi Lo ιo 0J Lπ μ LD ιt* c3. l cn tθ LD to Lπ μ _ vj ι o ω μ ro i- oi -) - M -J - θ - c . _ m „ ω w vi P -j ι - - o ^ _ ^ Ln o _ ffi „ μ (- ij (B cn oo ι μ μ ι ] ι ! Lo μ rf* μ ι cπ rf* bo _Λ μ LD oo co 3 t>θ ιf* LD j μ Lo o oι υ. co o Lθ Lo oo μ μ μ ιi* LD co θJ Lo co oo μ ιi* cn t ι^ co co co oo oo co co oo oo co co oo co oo oo oo oo co oo oo oo co co oo co co co co co co oo oo co oo co oo oo co co σ-. LΛ LΛ 4*. 4-* LO LO to to o LΛ o LΛ © LΛ O LΛ. O LΛ μ r- μr- μr- μi— μi— r- μi— μ r- μr- r- μr- μr- μi- μi- μr- μr- μr- r- μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ Cπ cπ cπ cπ w cπ cπ cn cπ cn cπ cπ cπ cπ ιl rf* rf* ιf* ιt ιt ιt* ιt* ιl rf* rf^ μ μ μ μ θ O O O O O O O O O l-0 i-> L0 l-3 L0 LD L0 L0 L0 L0 00 00 C0 00 00 00 00 ∞ ∞ ∞ -J ] l ! ] l l ! O ! Cn cn _^ co to μ o Lo ∞ ι cn cπ rf* co to μ o Lo co ! cn cπ ι[* co ιo μ o ι_> oo ] cn cπ ιJ co ιo μ o Lo oo -o cn cπ ΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩΩ ΩΩΩΩΩΩΩΩΩΩOΩΩΩ Q Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Si 3 s s; s; s; s; s: s: s; s: -s: s; s; s; s: -s: -=s s; s: s: s ; s -3 s §; 3 -≤ -g s; S 3 s S ≤ s Si : s s ; s ≤ 5 a « S « « s -3 s, ϊS s: s s3 I*' S** U*" S:**' tE* 1 t*» JE*' S* 5 μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ Lπ cn cπ cn cπ cπ cn cπ ιt rf* ιl. ιt* tt rf* rf* ιf ιi ιl** co co co oj oo co oj ω ! σι Cπ rf* co No μ o i-o co -j σι cπ rf* co to μ o Lo co J cn Lπ ιt* oo to μ o i-θ Co ι cn cπ ιt* ω l I I μ μ i i μ μ μ to μ μ I μ μ i μ μ μ μ i μ μ i μ i μ i μ μ μ to μ μ μ ι μ μ μ to i i μ μ rf* cn co to LO LO cn co o to oo oo co co cn cπ cπ oo co to σι co cπ μ to cn cn l o oj cn i rf* oj oo o tO O O O OO tO Cn μ θ O U. CO Cn LD LD I OJ Cπ CO tO ιt-. OJ rf* o rf* μ to LD cn -θ rf* to o cπ o o. co μ oo o -J oo o cj ι o rf* co cπ rf* cn t rf* rf* o o co to -j Lo oo -j oo o o oo cn o o μ o oo cn cπ cπ o cπ rf* co ι μ oo to rf* to oo oo ι cn μ μ cn oo to LD μ μ tθ Lo μ Lo o μ oo μ μ μ oo rf* μ co o o LD Lπ cn co rf* ι ι cn μ oo LD Lo oo rf* rf* LD μ oo cn - to ι cπ cπ co co o oJ -. cπ cn ι to rf* μ cπ μ o LD Co ι oπ ι cn co to to cπ cπ co μ υo cπ i rf* cn cn rf* μ ι σ. co μ -o Lπ -j -o cπ oJ rf* o ι co -j oo to σι Lo co ^ i Lπ ιo cn Lo co cn cn co cπ ι co cπ ιi^ σι cπ ιt* l cπ rf* ιt* _^ σ. ιf* cn ιl* cn co ιt* o j rf* co ιt* ιi^ ιi-> ιt* c^ o cπ cn ι cπ cn cn o o Lo μ μ -o o o o o cπ LD θo to o cπ co o μ ι μ cπ to o μ ι cπ o cπ LD Uι μ cn μ l LD LD Ljι cπ ιf* co c^ _ „ ui M ra ιt* Ln ω m m μ iλJ -) „ c. ∞ σι μ -) - _ ιt* θ - _ μ c, ^ o) ∞ - θ. _ t oo cπ o co oo rf* to ι-3 ιt* ιi-. Lo to μ co ι oo co cn cπ Lπ to ι cπ cπ oo cπ cπ co Lo co cπ l Lo cn cn to rf* i Lθ Lo co cn μ co oo ιt* LD Lo o Lo co o co J io ι-o cπ μ o co -J cn ω to to i μ i μ to to μ 1 1 1 μ μ to μ t μ co i co to μ to to i μ to μ μ i to to μ to to oJ i co to ! co i μ to μ l μ to oo to to μ bo co o μ μ co cπ μ cn o rf * μ co ! cn 00 o ι μ I μ ιt* oJ θJ θJ to μ vD θ θ co cπ o o co to μ cπ co rf* ^ j o co oo to Lo cn oo to o oo oo i cn rf* D LD μ LD Lo μ o o μ μ io Lo o cπ μ to -o cπ LD ιt LD tθ Lo ! o ! Lo Lπ μ o to μ -J θ3 tθ ιi oo Lo oJ cn i-> σι tθ ιi oo o ι tθ Lπ cπ ] to ∞ co cπ ~j -J cooo oo oo l t rf* cn o o i Lθ θ rf* to cπ rf* o -j Lθ rf* cn μ t rf* cn-cπ ι_) θ cπ -θ LD Co o μ o oo o ι oo LD θ Lo co ιi** cn ι co μ oo oo o -o co ι cn rf* co μ co rf* ιt* θ Lθ rf* μ tO LD LO Cn θ LD ( l rf* 1 00 CO LD tO CO O Oo μ θ o . owc ; .oi - ino μuι _σιιj. - M - ^μcoωtnιt * o μ iMo^uιtθ - t- _ co μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ oo ooooo ooo ooooo oo ooo oo ooooooooooooo ooooo oooooooooo oo ooo oo ooo o o oooo ooooooo ooooo o oo oo oooooooo ooooo oooooooooooo ooo ooooooo oooo cn Lπ _Λ ιt*- ι|ι> cπ cπ cπ ιt* cπ cπ ιt* ιl* ιt* ιf* cπ cπ ιf* _^ cπ ιf* rf* ι(* ω t ιf* ] -o cD ι o cD Co ] to LD oo Lo -j to oo cπ co o j ι-o ι ] oo co uo cn i Lo oo cπ o μ o cn c-. μ rf^ μ -n μ co to LD Lo rf* cπ oo to o o to to μ o o μ μ o -- _ o ι. ιt* tn (Λ -) ω c. o o Ln μ m μ -J θ _ „ _ -) o - c. -J c, si oι o _ - _ - c . ι* ω co o ι oo ιi-. iD Lπ μ oo Lo o cn LD oo cn co -j o o o rf* cn LD ιi* oo i (ji Lπ o^ cπ LD μ cn μ co co to cn μ ι o cπ cπ rf* cn ιl* μ o μ co rf* co o cπ co μ rf* o μ ιt^ co oo oo co co co co co oo oo oo oo oo co co oo αo oo oo co co cσ oo oo co co co co oo oo oo oo oo α- rø LO LO to to LΛ O LΛ © LΛ LΛ B Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω g:3£Kgg g:3 !3gl3gl3 !3KK gl3 : K!3g μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ μ Ln cjπ cπ cπ cπ cn cπ Lj. cπ cπ cπ cπ cn cn cπ cπ cπ cn cπ cn cπ cπ cπ Lπ cπ cπ cn cπ cπ cn cπ cπ cπ cπ cπ cn Lπ ιl*. ιt* ll- * rf* lt* * rf* lt* ll*. ll* rf* 00 00 00 C C0 C0 0J 00 00 00 t tO ιO M tO tO tO tO M o - _ i o, -i ^ _ _ μ o - ffi ^ -i w ιt* _ M μ o - _ ι m uι ^ _ -j μ o - co -j m -i -* Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω Ω S3 3 S3 S3 _s S3 s g ≤ s ≤ s s: ≤ s; s: s; s; s; s; s s s; ≤ s; s s; s; s s; s s s; -s: s; ≤ S S3 S S3 S S3 3 S3 S3 S S3 β S3 S3 S S3 S3 5 S S3 ' 1 ' ' |)-i ' ij ;3 S S S S ≤ ;> | [) [ f S3 S3 S S >g t >? i >f ' ι ' iϊ> , | | ' , i >ι 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 - 3 - 3 3 " 3 " 3 3 3 3 - 3- 3- 3- 3- 3 3 3 ^- ^ " ^- ^ " S S3 S S3 S3 S S S3 S S3 S3 S3 3 S ≤ S S ≤ 3 S3 ?3 S S S3 3 S μ μμ μμμ μμ μ μμμμ μμ μ μ μ μμμ μμμμμ μ μ μμμ μμ μ μ μμ _ _ _ _ _ co ra co co co oo (xι ω co !iι ι i vi ι ι o ι θ vi σ\ σι oι m m cτι cιi m ιt* ω -j μ o - co ^ m -i (* _ to μ o - _ ι m -i pr * u u μ o - oo ι σι ui -* - (o μ o -) co I t I I μ to to i t I μ to μ to μ I i i i to μ to i i to μ i μ i cn cn to μ μ to μ to oo o o Cn cn rf* to cn io _o to μ to co cn co cn o μ cn μ o n co rf* oo rf* to cπ μ co co oo cπ oo Lo o ι to co ιo o j |o l rf*. ι cn μ oo ~o co to co Lo Lπ cn μ to -o -j ι cπ oo t rf* cn oo LO i Lo rf* LD i to cn cn cn i LD LD cn co ι o rf* Lo cn Lθ Lo μ cn Lo rf* ι oo oo cπ LD rf* ιi* -θ rf* cπ Lθ Co μ Lo cπ o co co -o to ι ι θj μ ι o oo rf . tf* cn o co cn cn it* rf* if* i -o. co cn -o. LD it* rf* rf* rf* rf* co ιf_ oJ cπ ιt* co Lπ cn ιf* cπ ι cπ ιi* cn cn cπ cπ i ιl* * cπ rf* co cπ cπ co oJ ιt* cπ oo to ω Lo ιt* ιt* μ μ cn oJ cn cn μ t>o cπ ι cn ! ι cn o o o cn μ cD to cn to o ' LD Co cπ o o cn μ co ιi_. cn μ cπ μ ι cn LD Co LD t cπ LD oo LD to to co cn cn LD θJ to μ LD C i rf* cn cπ Lθ cn to to cπ cπ μ oo o μ oo cπ oJ -o cπ o cπ Lo oo o μ i rf* rf* o LD rf*. co oJ to μ ι cπ co to rf* cn Lo cn to cπ o -j cn μ -J co cn μ co o co μ to oo μ Lo μ i rf* ι cn ι ι -J co μ o cn o Lo oo cn cn o co oj i ii-. on co oj I μ μ co to μ μ l i to μ μ ι to μ to i to μ to to u μ μ w to o co μ to rf* LD co co oo oo to cn cn μ μ co ιt* cn cn -θ -o cn oo o cπ cπ Lo LD to oo Lθ cπ i Lθ θ rf* LD -j o ∞ μ co o μ cπ μ oo o ι cπ cn cπ co -j cπ ι μ ιi** rf* LD -J ! -o -j cπ μ LD μ rf* cπ ι cπ co ^ ra uι m -J tι co co σι co t M αι ^ i σι uι μ - uι μ _ uι ^ ω o μ ιθ -θ ^ m - ) ω l o σi -) ι μ Lo cn o μ L_ o cn cn co cπ LD LD j LJi μ co oj σ cπ LD ιt* o o o co co co rf* cn co co cπ cπ o o μ μ μ μ μ μ μ μ μ μ μ μ μ μμ μ μ μ μ μ μ o o o ooooooooooooooooooo ooooo ooooo ooo o o o ooo ooooo ooooooo ooo oo ooooo oooo ooooo cn cn cn cn cn cn c" cr. cn cn cn cn cn cn on cri cn cri cn cn cn Ln θJ o to co to cn to M to μ i Lo LD - cn o rf* μ μ μ cπ o oo cπ o ι -J θ ιt* L co cn cn u3 LD LD to co oo oo oo co co ro oo co oo oo oo co co co oo co co oo co co oo co oo oo oo oo oo oo oo oo oo co co co oo oo

Claims: CLAIMS 1. A computer-based method for identifying a ligand for CD81, comprising the steps of: (a) providing a 3D structural representation of the large extracellular loop of CD81 in a storage medium on a computer; and (b) using the computer to apply structure-based drug design techniques to the structural representation. 2. The method claim 1, wherein the structure-based drug design includes one or more steps of docking. 3. The method of claim 2, wherein the docking step screens members of a structural library of potential ligands. 4. The method of any preceding claim, wherein the structure-based drug design includes the definition of a pharmacophore. 5. The method of any preceding claim, wherein the structure-based drag design includes de novo drug design. 6. The method of any preceding claim, wherein the 3D structural representation includes van der Waals contacts, electrostatic interactions, and/or hydrogen bonding opportunities on the CD81 surface. 7. The method of any preceding claim, wherein the 3D structural representation is derived from the atomic co-ordinates cdδllel.pdb, or a variant of cd811el.pdb in which the r.m.s. deviation of the x, y and z co-ordinates for all heavy atoms is less than 2.5A 8. The method of any preceding claim, wherein the storage medium is RAM or ROM. 9. The method of any preceding claim, comprising the further steps of: (c) providing a compound identified by said structure-based drag design techniques; and (d) contacting said compound with CD81, or a fragment thereof containing the LEL, and assaying the interaction between them. 10. A compound identified using the method of any one of claims 1 to 9. 11. A compound identified using the method of any one of claims 1 to 9 for use as a pharmaceutical. 12. The use of a compound identified using the method of any one of claims 1 to 9 in the manufacture of a medicament for treating hepatitis C infection. 13. A method of treating a patient with hepatitis C infection, comprising administering an effective amount of a compound identified using the method of any one of claims 1 to 9. 14. A computer-readable medium, characterised in that the medium contains atomic coordinates and/or a 3D structural representation of the LEL of CD81. 15. The medium of claim 9, wherein the atomic co-ordinates are cdδlleLpdb. 16. A composition comprising an extracellular region of CD81 in crystalline form. 17. The composition of claim 16, wherein the crystal has monoclinic space group P2 1 (a = 31.5A, b = 11.2k, c = 38.5A, = 107.4°). 18. The composition of claim 16, comprising a co-crystallised ligand.

Inventors: BOLOGNESI MARTINO, GRANDI GUIDO, BOLOGNESI, MARTINO, GRANDI, GUIDO
Applicants: BOLOGNESI MARTINO, CHIRON SPA, GRANDI GUIDO, BOLOGNESI, MARTINO, GRANDI, GUIDO
Application year: 2001
Main classification: C07K14
IPC codes: C07K14, G01N33, G06F19