MACROCYCLIC NS3-SERINE PROTEASE INHIBITORS OF HEPATITIS C VIRUS COMPRISING ALKYL AND ARYL ALANINE P2 MOIETIES

Abstract: The present invention discloses novel macrocyclic compounds which have HCV protease inhibitory activity as well as methods for preparing such compounds. In another embodiment, the invention discloses pharmaceutical compositions comprising such macrocycles as well as methods of using them to treat disorders associated with the HCV protease.

Description: Macrocyclic NS-3 Serine Protease Inhibitors of Hepatitis C Virus comprising Alkyl and Aryl Alanine P2 Moieties Field of invention The present invention relates to novel hepatitis C virus ("HCV") protease inhibitors, pharmaceutical compositions containing one or more such inhibitors, methods of preparing such inhibitors and methods of using such inhibitors to treat hepatitis C and related disorders. This invention specifically discloses novel macrocyclic compounds as inhibitors of the HCV NS3/NS4a serine protease. The disclosure herein is related to that in pending patent application, Serial No. , filed April 5, 2000. Background of the invention Hepatitis C virus (HCV) is a (+)-sense single-stranded RNA virus that has been implicated as the major causative agent in non-A, non-B hepatitis (NANBH), particularly in blood-associated NANBH (BB-NANBH)(see, International Patent Application Publication No. WO 89/04669 and European Patent Application Publication No. EP 381 216). NANBH is to be distinguished from other types of viral-induced liver disease, such as hepatitis A virus (HAV), hepatitis B virus (HBV), delta hepatitis virus (HDV), cytomegalovirus (CMV) and Epstein-Barr virus (EBV), as well as from other forms of liver disease such as alcoholism and primary biliar cirrhosis. Recently, an HCV protease necessary for polypeptide processing and viral replication has been identified, cloned and expressed; (see, e.g.. U.S. Patent No. 5,712,145). This approximately 3000 amino acid polyprotein contains, from the amino terminus to the carboxy terminus, a nucleocapsid protein (C), envelope proteins (E1 and E2) and several non-structural proteins (NS1 , 2, 3, 4a, 5a and 5b). NS3 is an approximately 68 kda protein, encoded by approximately 1893 nucleotides of the HCV genome, and has two distinct domains: (a) a serine protease domain consisting of approximately 200 of the N-terminal amino acids; and (b) an RNA-dependent ATPase domain at the C-terminus of the protein. The NS3 protease is considered a member of the chymotrypsin family because of similarities in protein sequence, overall three-dimensional structure and mechanism of catalysis. Other chymotrypsin-Iike enzymes are eiastase, factor Xa, thrombin, trypsin, plasmin, urokinase, tPA and PSA. The HCV NS3 serine protease is responsible for proteolysis of the polypeptide (polyprotein) at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a and NS5a/NS5b junctions and is thus responsible for generating four viral proteins during viral replication. This has made the HCV NS3 serine protease an attractive target for antiviral chemotherapy. It has been determined that the NS4a protein, an approximately 6 kda polypeptide, is a co-factor for the serine protease activity of NS3. Autocleavage of the NS3/NS4a junction by the NS3/NS4a serine protease occurs intramolecularly (le., cis) while the other cleavage sites are processed intermolecularly (i.e.. trans). Analysis of the natural cleavage sites for HCV protease revealed the presence of cysteine at P1 and serine at P1 ' and that these residues are strictly conserved in the NS4a/NS4b, NS4b/NS5a and NS5a/NS5b junctions. The NS3/NS4a junction contains a threonine at P1 and a serine at P1 '. The Cys→Thr substitution at NS3/NS4a is postulated to account for the requirement of cis rather than trans processing at this junction. See, e.g.. Pizzi et al. (1994) Proc. Natl. Acad. Sci (USA) _91_:888-892, Failla et al. (1996) Folding & Design 1 :35-42. The NS3/NS4a cleavage site is also more tolerant of mutagenesis than the other sites. See, e^, Kollykhalov et al. (1994) J. Virol. 68:7525-7533. It has also been found that acidic residues in the region upstream of the cleavage site are required for efficient cleavage. See, e^ Komoda et al. (1994) J. Virol. 68:7351-7357. Inhibitors of HCV protease that have been reported include antioxidants (see, International Patent Application Publication No. WO 98/14181 ), certain peptides and peptide analogs (see, International Patent Application Publication No. WO 98/17679, Landro et al. (1997) Biochem. 36:9340-9348, Ingallinella et al. (1998. Biochem. 37:8906-8914. Llinas-Brunet et al. (1998. Bioorg. Med. Chem. Lett. 8:1713-1718). inhibitors based on the 70-amino acid polypeptide eglin c (Martin et al. (1998) Biochem. 37: 1 1459-1 1468, inhibitors affinity selected from human pancreatic secretory trypsin inhibitor (hPSTI-C3) and minibody repertoires (MBip) (Dimasi etal. (1997. J. Virol. 71 :7461-7469). cV H E2 (a "camelized" variable domain antibody fragment) (Martin et al.(1997. Protein Enα. 10:607-614). and 1-antichymotrypsin (ACT)(Elzouki et al.. (1997) J. Hepat. 27:42-28). A ribozyme designed to selectively destroy hepatitis C virus RNA has recently been disclosed (see, BioWorld Today 9(217): 4 (November 10, 1998)). Reference is also made to the PCT Publications, No. WO 98/17679, published April 30, 1998 (Vertex Pharmaceuticals Incorporated); WO 98/22496, published May 28, 1998 (F. Hoffmann-La Roche AG); and WO 99/07734, published February 18, 1999 (Boehringer Ingelheim Canada Ltd.). HCV has been implicated in cirrhosis of the liver and in induction of hepatocellular carcinoma. The prognosis for patients suffering from HCV infection is currently poor. HCV infection is more difficult to treat than other forms of hepatitis due to the lack of immunity or remission associated with HCV infection. Current data indicates a less than 50% survival rate at four years post cirrhosis diagnosis. Patients diagnosed with localized resectable hepatocellular carcinoma have a five-year survival rate of 10-30%, whereas those with localized unresectable hepatocellular carcinoma have a five-year survival rate of less than 1 %. Reference is made to A. Marchetti et al, Synlett, S±, 1000-1002 (1999) describing the synthesis of bicylic analogs of an inhibitor of HCV NS3 protease. A compound disclosed therein has the formula: Reference is also made to WO 00/09558 (Assignee: Boehringer Ingelheim Limited; Published February 24, 2000) which discloses peptide derivatives of the formula: where the various elements are defined therein. An illustrative compound of that series is: Reference is also made to WO 00/09543 (Assignee: Boehringer Ingelheim Limited; Published February 24, 2000) which discloses peptide derivatives of the formula: where the various elements are defined therein. An illustrative compound of that series is: Current therapies for hepatitis C include interferon-α (INF < *) and combination therapy with ribavirin and interferon. See, e.g.. Beremguer et al. (1998) Proc. Assoc. Am. Physicians 110(2):98-112. These therapies suffer from a low sustained response rate and frequent side effects. See, e.g.. Hoofnagle et al. (1997) N. Enol. J. Med. 336:347. Currently, no vaccine is available for HCV infection. Pending patent application, Serial Number, , filed April 5, 2000, discloses certain macrocyclic compounds as inhibitors of the HCV protease as well as pharmaceutical compositions containing said compounds. There is a need for new treatments and therapies for HCV infection. It is, therefore, an object of this invention to provide compounds useful in the treatment or prevention or amelioration of one or more symptoms of hepatitis C. It is a further object herein to provide methods of treatment or prevention or amelioration of one or more symptoms of hepatitis C. A still further object of the present invention is to provide methods for modulating the activity of serine proteases, particularly the HCV NS3/NS4a serine protease, using the compounds provided herein. Another object herein is to provide methods of modulating the processing of the HCV polypeptide using the compounds provided herein. Summary of the invention In its many embodiments, the present invention provides a novel class of macrocyclic inhibitors of the HCV protease, pharmaceutical compositions containing one or more of the compounds, methods of preparing pharmaceutical formulations comprising one or more such compounds, and methods of treatment, prevention or amelioration or one or more of the symptoms of hepatitis C. Also provided are methods of modulating the interaction of an HCV polypeptide with HCV protease. Among the compounds provided herein, compounds that inhibit HCV NS3/NS4a serine protease activity are preferred. The presently disclosed compounds generally contain about four or more amino acid residues and less than about twelve amino acid residues. In its principal embodiment, the present invention provides a macrocyclic compound of Formula I: wherein: E, X and Y may be independently present or absent, and if present are independently selected from the moieties: alkyl, aryl, alkyl-aryl, heteroalkyl, heteroaryl, aryl-heteroaryl, alkyl-heteroaryl, cycloalkyl, alkyl ether, alkyl-aryl ether, aryl ether, alkyl amino, aryl amino, alkyl-aryl amino, alkyl sulfide, alkyl-aryl sulfide, aryl sulfide, alkyl sulfone, alkyl-aryl sulfone, aryl sulfone, alkyl-alkyl sulfoxide, alkyl-aryl sulfoxide, alkyl amide, alkyl-aryl amide, aryl amide, alkyl sulfonamide, alkyl-aryl sulfonamide, aryl sulfonamide, alkyl urea, alkyl-aryl urea, aryl urea, alkyl carbamate, alkyl-aryl carbamate, aryl carbamate, alkyl -hydrazide, alkyl-aryl hydrazide, alkyl hydroxamide, alkyl-aryl hydroxamide, alkyl sulfonyl, aryl sulfonyl, heteroalkyl sulfonyl, heteroaryl sulfonyl, alkyl carbonyl, aryl carbonyl, heteroalkyl carbonyl, heteroaryl carbonyl, alkoxycarbonyl, aryloxycarbonyl, heteroaryloxycarbonyl, alkylaminocarbonyl, arylaminocarbonyl, heteroarylaminocarbonyl or a combination thereof, with the proviso that E, X and Y may optionally be additionally substituted with moieties selected from the group consisting of aromatic, alkyl, alkyl-aryl, heteroalkyl, aryl-heteroaryl, alkyl- heteroaryl, cycloalkyl, alkyl ether, alkyl-aryl ether, alkyl sulfide, alkyl-aryl sulfide, alkyl sulfone, alkyl-aryl sulfone, alkyl amide, alkyl-aryl amide, alkyl sulfonamide, , alkyl amines, alkyl-aryl amines, alkyl-aryl sulfonamide, alkyl urea, alkyl-aryl urea, alkyl carbamate, alkyl-aryl carbamate, halogen, hydroxyl amino, alkyl carbazate, aryl carbazate; R 1 = COR 5 or B(OR) 2 , wherein R 5 = H, OH, OR 8 , NR 9 R 10 , CF 3 , C 2 F 5 , C 3 F 7 , CF 2 R 6 , R 6 , COR 7 wherein R 7 = H, OH, OR 8 , CHR 9 R 10 , or NR 9 R 10 , wherein R 6 , R 8 , R 9 and R 10 are independently selected from the group consisting of H, alkyl, aryl, heteroalkyl, heteroaryl, cycloalkyl, cycloalkyl, arylalkyl, heteroarylalkyl, CH(R )COOR 11 , CH(R 1' )CONR 12 R 13 , CH(R 1 )CONHCH(R 2' )COO R 11 , CH(R 1' )CONHCH(R 2' )CONR 12 R 13 , CH(R 1 )CONHCH(R 2' )R', CH(R 1' )CONHCH(R 2' )CONHCH(R 3' )COO R 11 , CH(R 1 ' )CONHCH(R 2' )CONHCH(R 3' )CONR 12 R 13 , CH(R ' )CONHCH(R 2' )CONHCH(R 3' )CONHCH(R 4' )COO R 11 , CH(R ' )CONHCH(R 2' )CONHCH(R 3' )CONHCH(R 4' )CONR 12 R 13 , CH(R 1 ' )CONHCH(R 2' )CONHCH(R 3' )CONHCH(R 4' )CONHCH(R 5' )COO R 11 , CH(R 1' )CONHCH(R 2' )CONHCH(R 3' )CONHCH(R 4' )CONHCH(R 5' ) CONR 12 R 13 , wherein R 1' , R 2' , R 3' , R 4' , R 5' , R 11 , R 12 , R 13 , and R' are independently selected from a group consisting of H, alkyl, aryl, heteroalkyl, heteroaryl, cycloalkyl, alkyl-aryl, alkyl- heteroaryl, aryl-alkyl and heteroaralkyl; Z is selected from O, N, or CH; W may be present or absent, and if W is present, W is selected from C=O, C=S, SO 2 or C=NR; Q is (NR) P , O, S, CH 2 , CHR, CRR' or a double bond towards V; A is O, CH 2) (CHR) p , (CHR-CHR') p , (CRR') P> NR, S, SO 2 , C=O or a bond; G is (CH 2 ) P , (CHR) p, (CRR') P , NR, O, S, SO 2 , S(O) 2 NH, C=O, or a double bond towards E or V; V is CH, CR or N; p is a number from 0 to 6; and R, R', R 2 , R 3 and R 4 are independently selected from the group consisting of H; C1-C10 alkyl; C2-C10 alkenyl; C3-C8 cycloalkyl; C3-C8 heterocycloalkyl, aryl, alkoxy, aryloxy, alkylthio, arylthio, amino, amido, ester, carboxylic acid, carbamate, urea, ketone, aldehyde, cyano, nitro; heteroaryl; alkyl-aryl; alkyl- heteroaryl; (cycloalkyl)alkyl and (heterocycloalkyl)alkyl, wherein said cycloalkyl is made of three to eight carbon atoms, and zero to six oxygen, nitrogen, sulfur, or phosphorus atoms, and said alkyl is of one to six carbon atoms; with said alkyl, heteroalkyl, alkenyl, heteroalkenyl, aryl, heteroaryl, cycloalkyl and heterocycloalkyl moieties may be optionally substituted, with said term "substituted" referring to optional and suitable substitution with one or more moieties selected from the group consisting of alkyl, alkenyl, alkynyl, aryl, aralkyl, cycloalkyl, heterocyclic, halogen, hydroxy, thio, alkoxy, aryloxy, alkylthio, arylthio, amino, amido, ester, carboxylic acid, carbamate, urea, ketone, aldehyde, cyano, nitro, sulfonamide, sulfoxide, sulfone, sulfonyl urea, hydrazide, and hydroxamate and thiourea. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of skill in the art to which this invention belongs. Thus, for example, the term alkyl (including the alkyl portions of alkoxy) refers to a monovalent group derived from a straight or branched chain saturated hydrocarbon by the removal of a single atom having from 1 to 8 carbon atoms, preferably from 1 to 6; aryl - represents a carbocyclic group having from 6 to 14 carbon atoms and having at least one benzenoid ring, with all available substitutable aromatic carbon atoms of the carbocyclic group being intended as possible points of attachment. Preferred aryl groups include phenyl, 1 -naphthyl, 2-naphthyl and indanyl, and especially phenyl and substituted phenyl; aralkyl - represents a moiety containing an aryl group linked vial a lower alkyl; alkylaryl - represents a moiety containing a lower alkyl linked via an aryl group; cycloalkyl - represents a saturated carbocyclic ring having from 3 to 8 carbon atoms, preferably 5 or 6, optionally substituted; heterocyclic - represents, in addition to the heteroaryl groups defined below, saturated and unsaturated cyclic organic groups having at least one O, S and/or N atom interrupting a carbocyclic ring structure that consists of one or more rings, wherein each ring is 5-, 6- or 7-membered and may or may not have double bonds that lack delocalized pi electrons, which ring structure has from 2 to 8, preferably from 3 to 6 carbon atoms, e.g., 2- or 3-piperidinyl, 2- or 3-piperazinyl, 2- or 3-morpholinyl, or 2- or 3-thiomorpholinyl; halogen - represents fluorine, chlorine, bromine and iodine; heteroaryl - represents a cyclic organic group having at least one O, S and/or N atom interrupting a carbocyclic ring structure and having a sufficient number of delocalized pi electrons to provide aromatic character, with the aromatic heterocyclic group having from 2 to 14, preferably 4 or 5 carbon atoms, e.g., 2-, 3- or 4-pyridyl, 2- or 3-furyl, 2- or 3-thienyl, 2-, 4- or 5-thiazolyl, 2- or 4-imidazolyl, 2-, 4- or 5-pyrimidinyl, 2-pyrazinyl, or 3- or 4-pyridazinyl, etc. Preferred heteroaryl groups are 2-, 3- and 4-pyridyl; Such heteroaryl groups may also be optionally substituted. Also included in the invention are tautomers, rotamers, enantiomers and other optical isomers of compounds of Formula I, as well as pharmaceutically acceptable salts and solvates thereof. A further feature of the invention is pharmaceutical compositions containing as active ingredient a compound of Formula I (or its salt, solvate or isomers) together with a pharmaceutically acceptable carrier or excipient. The invention also provides methods for preparing compounds of Formula I, as well as methods for treating diseases such as, for example, HCV and related disorders. The methods for treating comprise administering to a patient suffering from said disease or diseases a therapeutically effective amount of a compound of Formula I, or pharmaceutical compositions comprising a compound of Formula I. Also disclosed is the use of a compound of Formula I for the manufacture of a medicament for treating HCV and related disorders. Detailed description of preferred embodiments In one embodiment, the present invention discloses compounds of Formula I as inhibitors of HCV protease, especially the HCV NS3/NS4a serine protease: where the various moieties are defined above. Some of the preferred embodiments include, but are not limited to, the following definitions of the various functionalities in the above-noted general formula I; other desired definitions for the same and additional functionalities may be found in the structures and claims of this application which are also within the contemplation of the present invention. Among the preferred embodiments, R 2 in formula I may be selected from the following moieties: E may be a substituted or unsubstituted alkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted heteroaryl or substituted or unsubstituted cycloalkyl, with preferred representations for E being: Preferred embodiments for R 3 include the moieties: wherein R 30 = H, CH 3 or other alkyl groups; R 31 = OH, O-alkyl, NH 2> N-alkyl; and R 32 and R 33 may be the same or different and are selected independently from H, F, CI, Br and CH 3 . Preferred embodiments for the moiety X-Y are the following structures: Several additional and further refinements of the above-noted various definitions for the compounds represented by Formula I are noted in the Claims section of this application. They are also represented by the various compounds listed in the specification and claims. Such refinements, definitions and limitations are to be considered as representing the entire invention of this application. Representative compounds of the invention which exhibit excellent HCV protease inhibitory activity are listed below: 10 11 13 15 16 17 18 19 20 23 24 25 27 29 30 31 32 33 36 37 38 39 40 41 43 44 51 The activity of some of the compounds are presented in Table 1 as ranges of Kj values in nanomolar (nM). The Example numbers in Table 1 refer to the numbers for the various structures in the EXAMPLES section found in the later parts of this application. Table 1 : HCV protease continuous assay results HCV continous assay Ki* range: Category A= 0.001 -1.0 μM, Category B= 1.1-100 μM Some methods of synthesizing the various types of the inventive compounds are described later in this section, and also schematically described, followed by the illustrative Examples. Depending upon the structure, the compounds of the invention may form pharmaceutically acceptable salts with organic or inorganic acids, or organic or inorganic bases. Examples of suitable acids for such salt formation are hydrochloric, sulfuric, phosphoric, acetic, citric, oxalic, malonic, salicylic, malic, fumaric, succinic, ascorbic, maleic, methanesulfonic and other mineral and carboxylic acids well known to those skilled in the art. For formation of salts with bases, suitable bases are, for example, NaOH, KOH, NH 4 OH, tetraalkylammonium hydroxide, and the like. In another embodiment, this invention provides pharmaceutical compositions comprising the above-described inventive macrocycles as an active ingredient. The pharmaceutical compositions generally additionally comprise a pharmaceutically acceptable carrier diluent, excipient or carrier (collectively referred to herein as carrier materials). Because of their HCV inhibitory activity, such pharmaceutical compositions possess utility in treating hepatitis C and related disorders. In yet another embodiment, the present invention discloses methods for preparing pharmaceutical compositions comprising the inventive macrocycle compounds as an active ingredient. In the pharmaceutical compositions and methods of the present invention, the active ingredients will typically be administered in admixture with suitable carrier materials suitably selected with respect to the intended form of administration, i.e. oral tablets, capsules (either solid-filled, semi-solid filled or liquid filled), powders for constitution, oral gels, elixirs, dispersible granules, syrups, suspensions, and the like, and consistent with conventional pharmaceutical practices. For example, for oral administration in the form of tablets or capsules, the active drug component may be combined with any oral non-toxic pharmaceutically acceptable inert carrier, such as lactose, starch, sucrose, cellulose, magnesium stearate, dicalcium phosphate, calcium sulfate, talc, mannitol, ethyl alcohol (liquid forms) and the like. Moreover, when desired or needed, suitable binders, lubricants, disintegrating agents and coloring agents may also be incorporated in the mixture. Powders and tablets may be comprised of from about 5 to about 95 percent inventive composition. Suitable binders include starch, gelatin, natural sugars, corn sweeteners, natural and synthetic gums such as acacia, sodium alginate, carboxymethylcellulose, polyethylene glycol and waxes. Among the lubricants there may be mentioned for use in these dosage forms, boric acid, sodium benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include starch, methylcellulose, guar gum and the like. Sweetening and flavoring agents and preservatives may also be included where appropriate. Some of the terms noted above, namely disintegrants, diluents, lubricants, binders and the like, are discussed in more detail below. Additionally, the compositions of the present invention may be formulated in sustained release form to provide the rate controlled release of any one or more of the components or active ingredients to optimize the therapeutic effects, i.e. HCV inhibitory activity and the like. Suitable dosage forms for sustained release include layered tablets containing layers of varying disintegration rates or controlled release polymeric matrices impregnated with the active components and shaped in tablet form or capsules containing such impregnated or encapsulated porous polymeric matrices. Liquid form preparations include solutions, suspensions and emulsions. As an example may be mentioned water or water-propylene glycol solutions for parenterai injections or addition of sweeteners and pacifiers for oral solutions, suspensions and emulsions. Liquid form preparations may also include solutions for intranasal administration. Aerosol preparations suitable for inhalation may include solutions and solids in powder form, which may be in combination with a pharmaceutically acceptable carrier such as inert compressed gas, e.g. nitrogen. For preparing suppositories, a low melting wax such as a mixture of fatty acid glycerides such as cocoa butter is first melted, and the active ingredient is dispersed homogeneously therein by stirring or similar mixing. The molten homogeneous mixture is then poured into convenient sized molds, allowed to cool and thereby solidify. Also included are solid form preparations which are intended to be converted, shortly before use, to liquid form preparations for either oral or parenterai administration. Such liquid forms include solutions, suspensions and emulsions. The compounds of the invention may also be deliverable transdermally. The transdermal compositions may take the form of creams, lotions, aerosols and/or emulsions and can be included in a transdermal patch of the matrix or reservoir type as are conventional in the art for this purpose. Preferably the compound is administered orally. Preferably, the pharmaceutical preparation is in a unit dosage form. In such form, the preparation is subdivided into suitably sized unit doses containing appropriate quantities of the active components, e.g., an effective amount to achieve the desired purpose. The quantity of the inventive active composition in a unit dose of preparation may be generally varied or adjusted from about 1.0 milligram to about 1 ,000 milligrams, preferably from about 1.0 to about 950 milligrams, more preferably from about 1.0 to about 500 milligrams, and typically from about 1 to about 250 milligrams, according to the particular application. The actual dosage employed may be varied depending upon the patient's age, sex, weight and severity of the condition being treated. Such techniques are well known to those skilled in the art. Generally, the human oral dosage form containing the active ingredients can be administered 1 or 2 times per day. The amount and frequency of the administration will be regulated according to the judgment of the attending clinician. A generally recommended daily dosage regimen for oral administration may range from about 1.0 milligram to about 1 ,000 milligrams per day, in single or divided doses. Some useful terms are described below: Capsule - refers to a special container or enclosure made of methyl cellulose, polyvinyl alcohols, or denatured gelatins or starch for holding or containing compositions comprising the active ingredients. Hard shell capsules are typically made of blends of relatively high gel strength bone and pork skin gelatins. The capsule itself may contain small amounts of dyes, opaquing agents, plasticizers and preservatives. Tablet- refers to a compressed or molded solid dosage form containing the active ingredients with suitable diluents. The tablet can be prepared by compression of mixtures or granulations obtained by wet granulation, dry granulation or by compaction. Oral gel- refers to the active ingredients dispersed or solubilized in a hydrophillic semi-solid matrix. Powder for constitution refers to powder blends containing the active ingredients and suitable diluents which can be suspended in water or juices. Diluent - refers to substances that usually make up the major portion of the composition or dosage form. Suitable diluents include sugars such as lactose, sucrose, mannitol and sorbitol; starches derived from wheat, corn, rice and potato; and celluloses such as microcrystalline cellulose. The amount of diluent in the composition can range from about 10 to about 90% by weight of the total composition, preferably from about 25 to about 75%, more preferably from about 30 to about 60% by weight, even more preferably from about 12 to about 60%. Disintegrant - refers to materials added to the composition to help it break apart (disintegrate) and release the medicaments. Suitable disintegrants include starches; "cold water soluble" modified starches such as sodium carboxymethyl starch; natural and synthetic gums such as locust bean, karaya, guar, tragacanth and agar; cellulose derivatives such as methylcellulose and sodium carboxymethylcellulose; microcrystalline celluloses and cross-linked microcrystalline celluloses such as sodium croscarmellose; alginates such as alginic acid and sodium alginate; clays such as bentonites; and effervescent mixtures. The amount of disintegrant in the composition can range from about 2 to about 15% by weight of the composition, more preferably from about 4 to about 10% by weight. Binder - refers to substances that bind or "glue" powders together and make them cohesive by forming granules, thus serving as the "adhesive" in the formulation. Binders add cohesive strength already available in the diluent or bulking agent. Suitable binders include sugars such as sucrose; starches derived from wheat, corn rice and potato; natural gums such as acacia, gelatin and tragacanth; derivatives of seaweed such as alginic acid, sodium alginate and ammonium calcium alginate; cellulosic materials such as methylcellulose and sodium carboxymethylcellulose and hydroxypropylmethylcellulose; polyvinylpyrrolidone; and inorganics such as magnesium aluminum silicate. The amount of binder in the composition can range from about 2 to about 20% by weight of the composition, more preferably from about 3 to about 10% by weight, even more preferably from about 3 to about 6% by weight. Lubricant - refers to a substance added to the dosage form to enable the tablet, granules, etc. after it has been compressed, to release from the mold or die by reducing friction or wear. Suitable lubricants include metallic stearates such as magnesium stearate, calcium stearate or potassium stearate; stearic acid; high melting point waxes; and water soluble lubricants such as sodium chloride, sodium benzoate, sodium acetate, sodium oleate, polyethylene glycols and d'l- leucine. Lubricants are usually added at the very last step before compression, since they must be present on the surfaces of the granules and in between them and the parts of the tablet press. The amount of lubricant in the composition can range from about 0.2 to about 5% by weight of the composition, preferably from about 0.5 to about 2%, more preferably from about 0.3 to about 1.5% by weight. Glident - material that prevents caking and improve the flow characteristics of granulations, so that flow is smooth and uniform. Suitable glidents include silicon dioxide and talc. The amount of glident in the composition can range from about 0.1 % to about 5% by weight of the total composition, preferably from about 0.5 to about 2% by weight. Coloring agents - excipients that provide coloration to the composition or the dosage form. Such excipients can include food grade dyes and food grade dyes adsorbed onto a suitable adsorbent such as clay or aluminum oxide. The amount of the coloring agent can vary from about 0.1 to about 5% by weight of the composition, preferably from about 0.1 to about 1%. Bioavailability - refers to the rate and extent to which the active drug ingredient or therapeutic moiety is absorbed into the systemic circulation from an administered dosage form as compared to a standard or control. Conventional methods for preparing tablets are known. Such methods include dry methods such as direct compression and compression of granulation produced by compaction, or wet methods or other special procedures. Conventional methods for making other forms for administration such as, for example, capsules, suppositories and the like are also well known. Another embodiment of the invention discloses the use of the pharmaceutical compositions disclosed above for treatment of diseases such as, for example, hepatitis C the like. The method comprises administering a therapeutically effective amount of the inventive pharmaceutical composition to a patient having such a disease or diseases and in need of such a treatment. As stated earlier, the invention includes tautomers, enantiomers and other stereoisomers of the compounds also. Thus, as one skilled in the art knows, some of the inventive compounds may exist in isomeric forms. Such variations are contemplated to be within the scope of the invention. Another embodiment of the invention discloses a method of making the macrocyclic compounds disclosed herein. The compounds may be prepared by several techniques known in the art. Representative illustrative procedures are outlined in the following reaction schemes. It is to be understood that while the following illustrative schemes describe the preparation of macrocycles predominately derived from mefø-tyrosine or lysine at the P2 position. Suitable substitution of any of both the natural and unnatural amino acids will result in the formation of the desired macrocycles based on such substitution. Abbreviations which are used in the descriptions of the schemes, preparations and the examples that follow are: THF: Tetrahydrofuran DMF: Λ/,Λ/-DimethyIformamide EtOAc: Ethyl acetate AcOH: Acetic acid HOOBt: 3-Hydroxy-1 ,2,3-benzotriazin-4(3H)-one EDCI: 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride NMM: /V-Methylmorpholine ADDP: 1 ,1 '-(Azodicarbobyl)dipiperidine DEAD: Diethylazodicarboxylate MeOH: Methanoi EtOH: Ethanol Et2θ: Diethyl ether Bn: Benzyl Boc: tert-Butyloxycarbonyl Cbz: Benzyloxycarbonyl Cp: Cylcopentyldienyl Ts: p-toluenesulfonyl Me: Methyl HATU: O-(7-Azabenzotriazol-1-yl)-N,N.N',N'-tetramethyluronium hexafluorophosphate Chg: Cyclohexylglycine Tyr: Tyrosine G: Glycerol TG: Thioglycerol alloc: allyloxycarbonyl FMOC: 9-Fluorenyl methyloxycarbonyl Dde: N-1-(4,4-dimethyl-2,6-dioxocyclohex-1-ylidene)ethyl tBu: terf-butyl equiv: equivalent rel. int.: relative intensity aq: aqueous rt: room temperature satd: saturated Hex: hexane(s) NBA: Nitrobenzoic acid PyBrOP: Tris(pyrrolidino)bromophosphonium hexafluorophosphate DMSO: Dimethyl sulfoxide TFA: Trifluoroacetic acid HOBt: Hydroxybezotriazole Hϋnigs base: Diisoprpylethyl amine BOP: Benzotrizaol-1 -yloxytris(dimethylamino)phosphonium hexafluorophosphate LDA: Lithium diisopropyl amide Ph3P: Triphenyl phosphine LAH: Lithium Aluminum Hydride DMAP: 4-Dimethyl aminopyridine DCC: Dicyclohexylcarbodiimide MCPBA: meta-Chloroperbenzoic acid BINAP: 2,2'-Bis(diphenylphosphino)-1 ,1'-binaphtol MeCN: acetonitrile Pr: Propyl Ac: Acetyi Ph: Phenyl General Schemes for Synthesis of Inventive Compounds: In the following Schemes, n is a number from 1 to 6. Scheme 1 : The synthesis of compounds of type 1e wherein R 1 , R 2 , R 3 ,R' are defined 4 above, R being amide, carbamate.or hydrogen, R being alkyl, aryl or arylalkyl, began with the coupling of la with the dipeptide lb using NMM , HOBt, and EDCI to obtain the intermediate Ic. The intermediate Ic was treated with Cs 2 CO 3 in DMF followed by photolysis to obtain compound id. The macrocyclic ester Id was hydrolyzed and coupled with a suitable amine intermediate to generate compounds of type 1e_. Scheme 2: 2e The preparation of the compound of formula 2e, wherein R " 1 , R^, R3 and n are defined above, R' is alkyl, heteroalkyl (OR", SR'", NR"R'" wherein R" and R"' are alkyl groups), halo substituent at ortho, meta, or para -position to oxygen atom, R is alkyl, aryl, or alkylaryl groups, and n is from zero to five, is outlined in Scheme 2. The meta-tyrosine-dipeptide 2a is coupled to an alkenyl carboxylic acid in the presence of HOOBt, EDCI-HCI and NMM. Hydroboration of the resulting product affords compound 2c. The macrocyclization is achieved under Mitsunobu conditions by using triphenylphosphine and ADDP. (The Mitsunobu reaction is reviewed by D. L. Hughes, Org. Reactions, 42 (1992) 335, John Wiley & Sons, New York, L. Paquette, ed.). After the ester is hydrolyzed to an acid with lithium hydroxide, it is coupled to an amine intermediate to afford 2e. Scheme 3: 1 2 3 The preparation of compounds of formula 3e wherein R', R , R , R , R and n are defined in Scheme 1 and PG is Cbz, Boc, or alloc is outlined in Scheme 3. The compound 3a was coupled with substituted histidine derivative using DCC. This compound 3b was deprotected and further treated with ω-bromo acids to obtain compound of the type 3c. Cyclization of 3c was accomplished with Nal and Na2CO3 in boiling methylvinyl ketone to afford 3d- The compound 3d was converted to compounds of type 3e by hydrolysis of the ester followed by coupling with the appropriate amine intermediate. Scheme 4: 4f 1 2 3 The preparation of the compounds of type 4f where in R', R , R , R , R, n and PG are defined in Scheme 1 was initiated from compound of the type 4a. The alcohol of 4a was converted to 4b_by treatment with phosgene. 4b was converted to 4c by coupling with alloc protected lb and EtβN. The alloc group of 4c_ was deprotected using Pd(PPh3)4 to obtain 4d which underwent cyclization under the Mitsunobu conditions to yield 4e. The ester of 4e was hydrolyzed and further coupled with an amine using EDC1, HOOBt to obtain compounds of the type 4f. Scheme 5: 1 9 3 The preparation of compounds of type 5h wherein R , R , R , R\ PG and n are defined in Schemes 1-3 and PG 1 being Cbz or Boc and PG 2 being alloc, began with the known compound 5a. The acid 5a was converted to the ester by refluxing it with ROH and TsOH. The phenolic oxygen of 5b was converted to the alloc group by the treatment with alloc-chloride and triethyl amine to yield 5c,. The secondary alcohol of 5c was converted to compounds of type 5d by coupling with protected cyclohexyl glycine using DCC and HOBt. The alloc group of compound 5d was deprotected using Pd(Ph 3 P) 4 , and dimedone. 5e was deprotected and treated with EDCI, HOOBt and appropriately activated ruthenium complex to obtain compounds of the formula of 5f. The compounds of the type 5f were converted to cyclic compounds of the formula 5g by the use of CS2CO3, and subsequent photolytic removal of ruthenium. The ester group of 5g was hydrolyzed and coupled to an amine intermediate to obtain compounds of the type 5h. Scheme 6: 6e 6f The preparation of the compound of formula 6f, wherein R1 , R2, R3 are defined above, R' is alkyl, heteroalkyl (OR", SR'", NR"R"' wherein R" and R'" are alkyl groups), halo substituent at ortho, meta, or para -position, R is alkyl, aryl, or alkylaryl groups, PG is Cbz or Boc, and n is from zero to five, is outlined in Scheme 6. The mefø-lodophenylglycine 6a is converted to its ester under usual esterification conditions (ROH, HCl). The product is then coupled to an N- protected amino acid in the presence of HOOBt, EDCI-HCI and NMM. After deprotection, the resulting amine is coupled again to a terminal alkenyl carboxylic acid to give product 6d. The intramolecular Heck reaction with a palladium catalyst provides the desired macrocyclic compound 6e. (The Heck reaction has been reviewed in detail by R. F. Heck, Org. React, 27 (1989) 345-390.). After the ester is hydrolyzed to an acid with lithium hydroxide, it is coupled to an amine intermediate to afford 6f. Scheme 7: 7b The preparation of the compound of formula 7b, wherein R1 , R2, R3 are defined above, R' is alkyl, heteroalkyl (OR", SR"', NR"R'" wherein R" and R'" are alkyl groups), halo substituent at ortho, meta, or para -position, R is alkyl, aryl, or alkylaryl groups, and n is from zero to five, is outlined in Scheme 7. The hydrogenation of the double bond of 6e gave the macrocycle 7a. Hydrolysis of the ester to acid and subsequent coupling with an amine intermediate afforded 7b. Scheme 8: δe δf The preparation of the compound of formula 8f, wherein R^ , R 2 , Rβ are defined above, R' is alkyl, heteroalkyl (OR", SR'", NR"R"' wherein R" and R"' are alkyl groups), halo substituent at ortho, meta, or para -position to oxygen atom, R is alkyl, aryl, or alkylaryl groups, PG is Boc, and n is from zero to five, is outlined in Scheme 8. The mefa-tyrosine-dipeptide 8a is coupled to a terminal Λ/-protected amino alcohol 8b under Mitsunobu conditions (triphenylphosphine and ADDP). The protecting groups are removed to give diamine hydrochloride 8dL The macrocyclization is achieved by forming the urea linkage using phosgene or carbonyl diimidazole. The resulting ester is then hydrolyzed to an acid with lithium hydroxide and is subsequently coupled to an amine intermediate to afford the desired product 8f. Scheme 9: The synthesis of compounds of the type 9d wherein the substituents R , 2 3 R , R , R', R and PG are defined in Scheme 1, was initiated by the deprotection of 9a with HCl in dioxane and coupling with ω-hydroxy acid using EDCI, HOOBt to yield compounds of the type 9b. The compounds of the type 9b were further cyclized using Ph 3 P, ADDP to generate compounds of the type 9c. The cyclic compound 9c was deprotected and coupled with an appropriate amine intermediate to generate compound of type 9d. Scheme 10: 10a 10b 10c 10d 10e 10h 10i The synthesis of the compounds of the type 101 wherein R 2 ', R 1 , R 1 , R 3 are defined in Scheme 1. PG 1 and PG 2 are defined as protecting groups namely Fmoc and Dde. P is defined as a polymer support wherein the compound is immobilized, and n is a number from 1-6. The methodology used for the synthesis of molecules of type 1PJ is a standard solid phase peptide synthesis with Fmoc protecting group. Fmoc protected Sasarin resin 10a is first deprotected by the treatment of piperidine followed by coupling with the Fmoc protected amino acid using HATU to obtain compound of type 10b. The protecting group of 10b was removed and coupled with amino acid to using HATU to obtain 10c. The polymer supported 10c was deprotected by treatment with piperidine and coupled with hydroxy acid to obtain hydroxy amide of type IQd.The protecting group of 10d was cleaved and coupled with protected lysine derivative using HATU to obtain compounds of type lOe. The protecting group of 10e was once again deprotected and coupled with Fmoc protected amino acid to obtain compound of the type lOf. The protecting groups PG 1 and PG 2 were removed and cyclzed using a diacid and HATU to obtain the macrocycle 10q. The compound of 10g_ was oxidized using Dess- Martin reagent and finally cleaved from the resin using TFA to obtain compound 10i. Scheme 11 : The synthesis of the compounds of type 11e wherein R 1 , R 2 , R 3 , R 4 and n are defined in Scheme 1 and PG 2 is Cbz, PG 1 is Bn and PG is Boc, was initiated from protected acid 11a. 11a was converted to compounds of type 11b by coupling with lysine derivative using EDCI, HOOBt methodology. The ester group of 11 b was hydrolyzed using LiOH.H2θ followed by the coupling with an appropriate amine intermediate to obtain the compound 11c. This was further treated with HCl in dioxane and coupled with lysine intermediate using EDCI, HOOBt to form compounds of the type lid. The compounds 11d were deprotected and cyclized using EDCI, HOOBt to form compounds of type 11e. Preparation of Intermediates: Intermediate A: Step 1 : To a stirred solution of 1-nitrobutane (16.5 g, 0.16 mol) and glyoxylic acid in H2O (28.1 g, 0.305 mol) and MeOH (122 mL) at 0°C-5°C, was added dropwise triethyl amine (93 mL, 0.667 mol) over 2 hrs. The solution was warmed to room temperature , stirred overnight and concentrated to dryness to give an oil. The oil was then dissolved in H2O and acidified to pH =1 with 10% HCl, followed by extraction with EtOAc. The combined organic solution was washed with brine, dried over Na2SO4, filtered and concentrated to dryness to give the product ii (28.1 g, 99% yield). Step 2: ii Hi To a stirred solution of starting material ii (240 g, 1.35 mol) in acetic acid (1.25 L) was added 10% Pd/C (37 g). The resulting solution was hydrogenated at 59 psi for 3 hrs and then at 60 psi overnight. The acetic acid was then evaporated and azeotroped 3 times with toluene, then triturated with MeOH and ether. The solution was then filtered and azeotroped twice with toluene to give iii as an off white solid (131 g, 0.891 mol, 66%). Step 3: BocHN τV H III IV To a stirred solution of the amino acid iii (2.0 g, 013.6 mmol) in dioxane (10 mL) and H2O (5mL) at 0°C, was added 1 N NaOH solution (4.3 mL, 14.0 mmol). The resulting solution was stirred for 10 minutes, followed by addition of di-f- butyldicarbonate (0.110 g, 14.0 mmol) and stirred at 0 °C for 15 minutes. The solution was then warmed to room temperature, stirred for 45 minutes and kept in refrigerator overnight and concentrated to dryness to give a crude material. To the solution of this crude material in EtOAc (100 mL) and ice, was added KHSO4 (3.36 g) and H2O (32 mL) and stirred for 4-6 minutes. The organic layer was then separated and the aqueous layer was extracted twice with EtOAc and the combined organic layer was washed with water, brine, dried over Na2SO4, filtered and concentrated to dryness to give the product iv as a clear gum (3.0 g, 89% yield). Step 4: iv v To a stirred solution of iv (3.00 g, 12.0 mmol) in DMF (15 mL) and CH2CI2 (15 mL) at -20°C was added HOOBt (1.97 g, 12.0 mmol), Λ/-methyI morpholine (4.0 mL, 36.0 mmol) and EDCI (2.79 g, 14.5 mmol) and stirred for 10 minutes, followed by addition of HCI-H2N-Gly-OBn (2.56 g, 13.0 mmol). The resulting solution was stirred at -20°C for 2 hrs, then kept in refrigerator overnight and concentrated to dryness, followed by dilution with EtOAc (150 mL). The EtOAc solution was then washed twice with saturated NaHCO3, H2O, 5% H3PO4, brine, dried over Na2SO4, filtered and concentrated to dryness to give the product v (4.5 g, 94%). LRMS m/z MH + = 395.1. Step 5: v vi The solution of starting material v (7.00 g, 17.8 mmol) in absolute ethanol (300 mL) was stirred at room temperature under a hydrogen atmosphere in the presence of Pd-C (300 mg, 10%). The reaction progress was monitored by tic. After 2h, the mixture was filtered through a celite pad and the resulting solution was concentrated in vacuo to give the product vi (5.40 g, quantitative). LRMS m/z MH + = 305.1. Step 6: To a solution of dimethylamine hydrochloride (1.61 g, 19.7 mmol), Λ/-Boc- phenylglycine (4.50 g, 17.9 mmol), HOOBt (3.07 g, 18.8 mmol) and EDCI (4.12 g, 21.5 mmol) in anhydrous DMF (200 mL) and CH2CI2 (150 mL) at -20°C was added NMM (5.90 mL, 53.7 mmol). After being stirred at this temperature for 30 min, the reaction mixture was kept in a freezer overnight (18 h). It was then allowed to warm to rt, and EtOAc ( 450 mL), brine (100 mL) and 5% H3PO4 (100 mL) were added. After layers were separated, the organic solution was washed with 5% H3PO4 (100 mL), saturated aqueous sodium bicarbonate solution (2 X 150 mL), water (150 mL), and brine (150 mL), dried (MgSO4), filtered and concentrated in vacuo to afford crude product viii (4.86 g) as a white solid, which was used without further purification. Step 7: VIII IX The Λ/-Boc-phenylglycine dimethylamide viii (4.70 g, crude) was dissolved in 4 N HCl (60 mL, 240 mmol) and the resulting solution was stirred at room temperature. The progress of the reaction was monitored by TLC. After 4 h, the solution was concentrated in vacuo to yield ix as a white solid which was used in the next reaction without further purification. LRMS m/z MH + = 179.0. Step 8: X The desired compound x was prepared according to the coupling procedures described in Step 4. LRMS m/z MH + = 465.1. Step 9: The desired intermediate A was prepared from tripeptide x according to the procedures described in Step 7. LRMS m/z MH + = 365.1. Intermediate B: Step 1 : The desired product xii was obtained by the procedure described for Intermediate A, Step 8 using commercially available xi as the coupling partner. The crude material was sufficiently pure for further studies. A portion of the product was purified by flash chromatography using 97/3 dichloromethane/MeOH. HRMS (FAB) Calcd for C-25H40N3O7: 494.2866 (M+H) + . Found: 494.2863. Step 2 The desired product B was obtained by the procedure described for Intermediate A, Step 7. The crude material was used without further purification. Intermediate C: Step 1 : The desired compound xiii was prepared according to the coupling procedures described in Step 6 for intermediate A. Step 2: The desired compound xiv was prepared according to the procedures described in Step 7 for intermediate A. Step 3: XIV VI The desired compound xv was prepared according to the coupling procedures described in Step 6 for intermediate A. LRMS m/z MH + = 451.1. Step 4: The desired intermediate C was prepared according to the procedures described in Step 7 for intermediate A. LRMS m/z MH + = 351.1. It was used without further purification. Intermediate D: The desired intermediate D was prepared from compound v according to the procedures described in Step 7 for intermediate A. It was used without further purification. Intermediate E: Step 1 : iv xvii xviii The desired product xviii was obtained by the procedure described for Intermediate A, Step 8 using commercially available xvii as the coupling partner. The crude material was sufficiently pure for further studies. Step 2 xviii E The desired product E was obtained by the procedure described for Intermediate A, Step 7. The crude material was used without further purification. Intermediate F: Step l The desired product xx was obtained by the procedure described for Intermediate A, Step 4 using commercially available xix as the coupling partner. The crude material was sufficiently pure for further studies. Step 2 The desired product F was obtained by the procedure described for Intermediate D. Intermediate G: Step l iv The desired product G was obtained by the procedure described for Intermediate A, Step 4 using allylglycine as the coupling partner. The crude material was sufficiently pure. The crude product was treated with 4N HCl/ Dioxane and stirred at rt. for 50 min. The reaction mixture was concentrated to dryness to yield intermediate G which was used without further purification. EXAMPLES Example 1 : Preparation of Compound of Formula 1 : Step A: A solution of 4-chloropropionic acid (2.0 g, 10.8 mmol) of 1a in dichloroethane (200 mL) was treated with CpRu(CH3CN)3 PF6 (4.7 g, 10.8 mmol, 1.0 equiv) and heated at reflux for 2 h. The reaction mixture was cooled to rt when colorless crystals of the product 1c precipitated out. The crystals were filtered and washed with 1 :1 mixture of E.2O/CH2CI2 and dried in vacuo. The colorless crystals (3.3 g) were analytically pure. H NMR (CD3C(O)CD3, 400 MHz, ppm, δ, J) 6.77 (d, 2 H, J = 7.0 Hz), 6.53 (d, 2 H, J =7 Hz), 5.64 (s, 5 H), 2.87 (t, 2 H, J =7.0 Hz), 2.74 (t, 2 H, J =7.0Hz); MS: (Electron spray, m/z relative intensity): 350.9 (Ci4Hi4CIRu + , M + , 100); CHN calcd for C14H 4CIF6O2PRU C=33.92% H=2.85% CI=7.15% P=6.25% found: C=34.04% H=3.04% Cl=7.09% P=5.71 %. Step B: A solution of Boc-cyclohexylgylcine monohydrate 1d (6.17 g, 24.00 mmol) in dry CH2CI2 (50.0 mL) was treated with 4-methylmorpholine (2.64 g, 26.0 mmol, 1.1 equiv.) and cooled to -10° C. To this mixture was added isobutyl chloroformate (3.62 g, 3.5 mL, 1.1 equiv.) and the white suspension was stirred until the bath temperature was -5 °C. mefa-Tyrosine methyl ester hydrochloride salt ( 6.5 g, 26.5 mmol, 1.1 equiv.) was dissolved in DMF (30 mL) in a separate beaker and treated with 4-methyl morpholine (2.64 g, 26.0 mmol, 1.1 equiv.) and stirred at rt. for 15 min. This mixture was added to the reaction which was accompanied by evolution of CO2. The reaction mixture was stirred at rt for 1 h and diluted with 1 M aq. HCl (100 mL). The aqueous layer was extracted with ethyl acetate (3x200 mL) and the combined organic layer was extracted with 1 M HCl (1x100 mL), aq NaOH (1x100 mL), brine (1x100 mL), dried (Na2SO4), concentrated in vacuo and purified by chromatography (Siθ2, EtOAc/Hexanes 3/7) to yield 5.3 g (53%) of coupled compound 1f as a colorless foam. Step C: A solution of 1f (10g, 23.04 mmol) was dissolved in HCl (4M solution in dioxane, 100 mL) and stirred at rt for 2-4 h. The reaction mixture was concentrated in vacuo and the solid was resuspended in ether. It was filtered and the solid was washed with ether which was dried to give a colorless solid 1g (8.2g, 96%) 1 H NMR (d4-CD3OD, 400 MHz, δ, ppm) 7.09 (t, 1 H, J=8.0 Hz), 6.71-6.36 (m, 3 H), 4.69 (dd, 1 H, J=6.0 Hz, 3.2 Hz), 3.69 (s, 3 H), 3.66 (d, 1 H, J=5.2 Hz), 3.15 -3.10 (dd, 1 H, J=5.6 Hz, 4.0 Hz), 1.87-1.69 (m, 6 H), 1.32-1.10 (m, 5 H). Step D: A solution of cyclopentadiene-η6-4-chlorophenylpropionic acid-ruthenium hexanesafluorophosphate 1c (2.0g, 4.0 mmol) in DMF (20 mL) was treated with HOBt (810 mg, 6.0 mmol, 1.5 equiv)and Hϋnigs base (2.6 g, 16.0 mmol, 4.0 equiv.) The reaction mixture was cooled to 0° C and treated with EDC HCI (888 mg, 5.0 mmol, 1.25 equiv.) The reaction mixture was stirred at 0 °C for 30 min and amine salt 1g (1.48 g, 4.0 mmol) was added to the mixture and stirred at rt for 12 h. The reaction mixture was concentrated in vacuo and the residue was diluted with H2O (200 mL) and extracted into CH2CI2 (3x100 mL). The combined organic layers were extracted with aq. HCl (1x100 mL), NaHCO3 (1x100 mL), brine (1x100 mL) and dried (Na2SO4), filtered concentrated in vacuo and the brown solid 1 h was used for cyclization without any further purification. Step E 1 h 1 i A solution of cyclopentadiene-η6-ruthenium-4-chlorophenylpropionic acid- cyclohexyglycine-t77e_a-tyrosine-OCH 3 1h (1.47 g crude) in dry DMF (150 mL) was treated with CS2CO3 (2.40 g, 7.37 mmol, 5.0 equiv) and degassed by bubbling dry N2 into the reaction mixture. The reaction mixture was stirred at rt for 16 h and the excess DMF was distilled off. The residue was dissolved in H2O (200 mL) and extracted with CH2CI2 (3x100 mL). The combined organic layer was extracted with brine (100 mL), dried (Na2SO4), filtered concentrated in vacuo and the residue was used for photolytic decomplexation of ruthenium without further purification. The crude ruthenium complex was dissolved in acetonitrile (35 mL), degassed and photolysed in a Raynot (λ=350 nM) for 48 h. The reaction mixture was concentrated in vacuo and the residue was purified by chromatography (Siθ2, EtOAc/Hexanes 7:3) to yield 360 mg (52%) of a colorless solid 1 i. Step F: 1 i 1j A solution of biphenyl ether 1i (300 mg, 0.65 mmol) in CH3OH (10 mL), CH2CI2 (20 mL) and H2O (5 mL) was treated with LiOH«H2θ (90mg, 2.2 mmol, 3.4 equiv.) and stirred at rt for 2 h. The reaction mixture was acidified with aq. HCl (6 M) and extracted into CH2CI2 (3x30 mL). The combined organic layers were dried (Na2SO4), filtered, and concentrated in vacuo to yield colorless acid 1j (200 mg, 66%). Step G: A solution of acid 1j (100 mg, 0.22 mmol) in dry DMF (2.5 mL) was treated with HOOBt (45 mg, 0.33 mmol) and Hϋnigs base (141 mg, 1.1 mmol, 5.0 equiv.) The reaction mixture was cooled to 0° C and treated with EDCI (63 mg, 0.33 mmol, 1.5 equiv) and stirred for 20 min. The reaction mixture was treated with amine B (118 mg, 0.27 mmol, 1.22 equiv.) and stirred at rt for 12 h. The reaction mixture was concentrated in vacuo and diluted with H2O (30 mL). The aqueous layer was extracted with CH2CI2 (3x50 mL) and EtOAc (3x50 mL). The combined organic layers were extracted with aq. HCl (2M), aq. NaOH (2M), dried (Na2SO4) filtered concentrated in vacuo to obtain a colorless solid 1k (79 mg) which was used for oxidation. MS: (Electron spray, m/z rel int): 826 [(M+1 ) + , 100], 494 (20), 94 (30). Step H: A solution of hydroxy amide 1k (130 mg, 0.16 mmol) in DMF (2.0 mL) was treated with Dess-Martin reagent (130 mg, 0.32 mmol, 2.0 equiv.). The reaction mixture was stirred at rt for 2 h and the mixture was concentrated in vacuo. The residue was purified by chromatography (Siθ2, CH3OH/CH2CI2: 1 :49) to yield oxidized product 1 (55 mg, 42%) as a colorless solid. MS: (Electron spray, m/z rel int): 858 [(M+CH3OH+1 ) + , 100], 824 [(M+1 )+ 63). Example 2: Preparation of Compound of Formula 2: Step A: A solution of tert-butyl ester 1 (50.0 mg, 60.0 μmol) was treated with TFA/CH2CI2 (1 :1 , 4 mL) and stirred at rt for 2 h. The disappearance of the ester to the base line was followed by TLC (CH3OH/CH2CI2 1 :24). After the deprotection was complete the reaction mixture was concentrated in vacuo and the residue was repeatedly treated with heptanes (4.0 mL) and concentrated to yield a white solid 2 (49 mg, 100%). MS: (Electron spray, m/z rel int): 768 [(M+1 ) + , 100). Example 3: Preparation of Compound of Formula 3: Step A: 3a A solution of acid 1j (100 mg, 0.22 mmol) in dry DMF (2.5 mL) was treated with HOOBt (45 mg, 0.33 mmol) and Hϋnigs base (141 mg, 1.1 mmol, 5.0 equiv.) The reaction mixture was cooled to 0° C and treated with EDCI (63 mg, 0.33 mmol, 1.5 equiv) and stirred for 20 min. The reaction mixture was treated with amine E (79 mg, 0.27 mmol, 1.22 equiv.) and stirred at rt for 12 h. The reaction mixture was concentrated in vacuo and diluted with H2O (30 mL). The aqueous layer was extracted with CH2CI2 (3x50 mL). The combined organic layers were extracted with aq. HCl (1 M, 30 mL) aq. NaOH (1 M, 30 mL), dried (Na2SO4) filtered concentrated in vacuo to obtain a colorless solid 3a (58 mg) which was used for oxidation. MS: (Electron spray, m/z rel int): 693 [(M+1 ) + , 100], 637 (41), 494 (55), 394 (51 ), 338 (13). Step B: A solution of alcohol 3a (95 mg, 0.14 mmol) in CH2CI2 (2.0 mL) was treated with Dess-Martin reagent (116 mg, 0.28 mmol, 2.0 equiv.) The reaction mixture was stirred at rt for 2 h and the mixture was concentrated in vacuo. The residue was purified by chromatography (Siθ2, CH3OH/CH2CI2 1 :32) to yield oxidized product 3 (47 mg, 42%) as a colorless solid. MS: (Electron spray, m/z rel int): 6 ^ (M+1 ) + . Example 4: Preparation of Compound of Formula 4: Step A: solution of tert-butyl ester 3 (47. mg, μmo was treated with HCl (4M Dioxane, 5 mL) and stirred at rt for 25 h. The disappearance of the ester to the base line was followed by TLC (CH3OH/CH2CI2 1 :24). After the deprotection was complete the reaction mixture was concentrated in vacuo and the residue was repeatedly treated with heptanes (5.0 mL) and concentrated to yield a white solid 4 (43 mg, 100%). MS: (Electron spray, m/z rel int): 635 [(M+1) + , 100)], 465 (62), 336 (62). Example 5: Preparation of Compound of Formula 5: Step A: A solution of 4-chlorobutyric acid 5a (3.0g, 15.10 mmol) in dichloroethane (200 mL) was treated with CpRu(CH3C )3PF6 1b (6.6 g, 15.10 mmol, 1.0 equiv) and heated at reflux for 2.5 h. The reaction mixture was cooled to 0°C and filtered. The filtrate was concentrated in vacuo and dissolved in CH3CN (10 mL) and treated with a large excess of Et2θ. The gum separating out was separated by decanting the ether and the residue was dissolved in CH2CI2/CH3OH (1 :1 , 100 mL) and concentrated in vacuo to obtain 5b as a brown gum which solidifies (3.5 g, 46%). Step B: 5c A solution of carboxylic acid 5b (3.12 g, 5.95 mmol) in dry DMF (20 mL) was treated with Hϋnigs base (3.07 g, 24.0 mmol, 4.0 equiv, 4.4 mL) and HOBt (1.2 g, 8.93 mmol, 1.5 equiv). The reaction mixture was cooled to 0°C and the treated with EDCI (1.35 g, 7.43 mmol, 1.25 equiv) and stirred for 1 h. To this reaction mixture was added amine hydrochloride 1g (2.65g, 7.14 mmol, 1.2 equiv), and the reaction mixture was stirred at rt for 12 h. DMF was distilled out and the residue was diluted with water and the aqueous layer was extracted with CH2CI2- The combined organic layers were extracted with aq NaHCO3, aq. HCl, brine, dried (Na2SO4), filtered, concentrated in vacuo and the crude product 5c (4.3 g) was used for cyclization without further purification. H NMR (d4-CD3OD, 400 MHz, δ, ppm) 7.35 (t, 1 H), 6.72-6.60 (m, 5 H), 6.33-6.20 (dd, 2 H), 5.51 (s, 5 H), 4.19 (d, 1 H), 3.68 (s, 3 H), 3.19-2.83 (m, 2 H), 2.51-2.40 (m, 2 H), 2.40-2.25 (m, 2 H), 1.99-1.59 (m, 8 H), 1.35-0.98 (m, 5 H); MS (FAB, NBA-G/TG-DMSO, m/z relative intensity) 695.3 ([M-PF6] + > 100), 232 (20), 171 (30); HRMS calcd for C34H42N2O5CIRU (M-PF6) 695.1832; found 695.1845. Step C: 5c 5d A solution of chloro-compound 5c (3.0 g 3.6 mmol) in dry DMF (300 mL) was degassed with dry 2 and CS2CO3 ( 5.2 g, 16 mmol, 4.0 equiv) and stirred at rt. for 16h. The solvent DMF was distilled off and the residue was diluted with water and extracted with CH2CI2 (3x100 mL) . The combined organic layers were dried (Na2SO4), filtered, concentrated in vacuo and dried under vacuum overnight. It was used for photolytic removal of Ru without further purification. MS FAB (NBA-G/TG-DMSO 695 ([M-PF6] + , 100]. The cyclized compound from the previous step was dissolved in CH3CN (35mL) and photolysed in a Raynot (λ=350 nm) for 48h. The reaction mixture was concentrated in vacuo and the residue was purified by chromatography (SiO2, EtOAc/Hexanes 1 :1 ) to yield a tan colored solid 5d (600 mg, 34%). 1 H NMR (CDCI3, 400 MHz, δ, ppm) 7.58 (d, 1 H, J=7.6 Hz), 7.14 (t, 1 H, J=8.0 Hz), 6.94 (d, 2 H, J=8.4 Hz), 6.87 (dd, 1 H, J=2.4, 5.6 Hz), 6.73 (d, 1 H,.J=7.2 Hz), 6.59 (s, 1 H), 6.57 (s, 2 H), 6.39 (d, 1 H, J=8.0 Hz), 4.51 (dt, 1 H, J=2.8, 8.0 Hz), 3.80-3.62 (m, 1 H), 3.62 (s, 3 H), 3.05-3.00 (dd, 1 H, J=2.8, 11.6 Hz), 2.85 (dd, 1 H, J=8.4, 6.0 Hz), 2.76-2.72 (m, 1 H), 2.36-2.19 (m, 3 H), 2.02 (dd, 1 H, J=6.4, 9.2 Hz), 1.8- 1.73 (m, 1 H), 1.61-1.34(m, 7H), ,1.41-0.71 (m, 7 H). MS (FAB, NBA-G/TG- DMSO, m/z relative intensity), 493 [(M+1 ) + , 100], 465 (20), 232 (30), 171 (40); HRMS calcd. for C29H37N2O5 (M+1 ) : 493.2702; found 493.2699. Step D: 5d 5e A solution of ether 5d (200 mg, 0.42 mmol) in CH3OH (5 mL), CH2CI2 (10 mL) and H2O (0.5 mL) was treated with LiOH»H2θ (18 mg, 0.44 mmol, 1.1 equiv.) and stirred at rt for 12 h. The reaction mixture was acidified with aqueous HCl (12 N, 1 mL) and concentrated in vacuo to yield acid 5e which was used directly for the coupling without further purification. Step E: A solution of acid 5e in dry DMF (5.0 mL) was treated with HOOBt (103 mg, 0.63 mmol, 1.5 equiv.), Hϋnigs base (216 mg, 1.68 mmol, 4.0 equiv.) and amine B (270 mg, 0.63 mmol, 1.47 equiv.) The reaction mixture was cooled to 0° C and treated with EDCI (101 mg, 0.52 mmol, 1.25 equiv) and stirred at rt for 12 h. The reaction mixture was concentrated in vacuo and diluted with H2O (30 mL). The aqueous layer was extracted with CH2CI2 (3x50 mL) and EtOAc (3x50 mL). The combined organic layers were extracted with aq. HCl (2M) aq. NaOH (2M), dried (Na2S04) filtered concentrated in vacuo to obtain a colorless solid 5f (177 mg) which was used for oxidation. MS: (Electron spray, m/z rel int): 840 [(M+1 ) , 100], 394 (100). Step F: A solution of alcohol 5f (177 mg, 0.21 mmol) in CH2CI2 (10.0 mL) was treated with Dess-Martin reagent (178 mg, 0.42 mmol, 2.0 equiv.) The reaction mixture was stirred at rt for 3 h and the mixture was concentrated in vacuo. The residue was purified by chromatography (Siθ2, CH3OH/CH2CI2 1 :49) to yield oxidized product 5 (23 mg, 13%) as a colorless solid. MS: (Electron spray, m/z rel int): 870 [(M+CH3OH+I) , 50], 838 [(M+1 ) + , 100). Example 6: Preparation of Compound of Formula 6: Step A: A solution of tert-butyl ester 5 (50.0 mg, 60.0 μmol) was treated with TFA/CH2CI2 (1 :1 , 4 mL) and stirred at rt for 7 h. The disappearance of the ester to the base line was followed by TLC (CH3OH/CH2CI2 1 :24). After the deprotection was complete the reaction mixture was concentrated in vacuo and the residue was repeatedly treated with heptanes (4.0 mL) and concentrated to yield a white solid 6 (14 mg, 100%). MS: (Electron spray, m/z rel int): 782 [(M+1 ) + , 100). Example 7: Preparation of Compound of Formula 7: Step A: A solution of alcohol 7a (9.2g, 54.1 mmol) in dry CH2CI2 (200 mL) was treated with DMSO (35 mL) and E.3N (16.4g, 16.3 mmol, 23.4 mL). The reaction mixture was cooled to 0 °C and treated with Py*SO3 (12.9 g, 81.2 mmol, 1.50 equiv.) dissolved in DMSO (30 mL). The reaction mixture was stirred at 0°C for 0.5 h and rt for 6 h. The reaction mixture was concentrated in vacuo and diluted with Et2θ (100 mL) and H2O (200 mL). The layers were separated and the aqueous layer was extracted with Et2θ (3x100 mL). The combined organic layers were extracted with HCl (2M, 3x100 mL), brine (1x100 mL) concentrated in vacuo and purified by chromatography (Siθ2, EtOAc/Hexanes 1 :7) to yield aldehyde 7b which solidified to a waxy solid on standing (7.1g, 77%). CHN calcd for C9H9CIO: C=64.11 % H=5.38%; found: C=64.08% H=5.30%. Step B: 7b 7c A solution of thiethylphosponoacetate (6.72g, 30 mmol, 1.2 equiv) in dry THF (100 mL) was treated with NaH (60% dispersion, 1.5g, 35 mmol, 1.4 equiv) at 0°C. The reaction mixture was stirred at 25°C for 1 h until the H2 evolution ceased. A solution of aldehyde 7b (4.2g, 25.0 mmol) in dry THF (5.0 mL) was added and the reaction mixture was stirred for 36 h. The reaction mixture was diluted with H2O (100 mL) and extracted with E_2θ (3x70 mL). The combined organic layer was dried (MgSO4), filtered, concentrated in vacuo and chromatographed to yield α,β-unsaturated ester 7c (4.2g, 71 %) which was used for reduction. Step C 7d 7c A solution of α,β-unsaturated ester 7c (4.2g, 8.0 mmol) in EtOAc (50 mL) was treated with Pd/C (10% w/w, 500 mg) and hydrogenated at 50 psi for 12 h. The reaction mixture was filtered through a plug of celite and the filtrate was concentrated in vacuo to yield the reduced compound 7d (3.9 g, 93%). Step D: 7d 7e A solution of the ester 7d (3.9 g, 16.2 mmol) in CH3OH/THF/H2O (1 :1 :0.1 , 110 mL) was treated with LiOH*H2θ (1.2 g, 30 mmol, 2.0 equiv) and stirred at rt for 5 h. The reaction mixture was concentrated in vacuo and the residue was diluted with H2O (100 mL) and extracted into Et2θ (3x50 mL). The aqueous layer was acidified to pH ~1 (13 M HCl) and the turbid aqueous layer was extracted with Et2θ (3x100 mL). The combined organic layers were dried (MgSO4) filtered concentrated in vacuo to yield a colorless solid 7e (3.1 g, 96%). CHN calcd for C11 H13CI02 C=62.12% H=6.16%; found: C=62.27% H=6.23%. 7e 7f A solution of 4-chlorophenyl pentanoic acid 7e (3.0g, 14.15 mmol) in dichloroethane (150 mL) was treated with CpRu(CH3CN)3PF6 1b (6.75 g, 15.10 mmol, 1.0 equiv) and heated at reflux for 2.5 h. The reaction mixture was cooled to 0° C and filtered. The filtrate was concentrated in vacuo and dissolved in CH3CN (20 mL) and treated with a large excess of Et2θ. The gum separating out was separated by decanting the ether and the residue was dissolved in CH2CI2/CH3OH (1 :1 , 100 mL) and concentrated in vacuo to obtain 7f a brown gum which solidifies (4.36 g, 58%). MS: (Electron spray, m/z rel int): 379 [(M- PF6) + , 100]. Step F: A solution of carboxylic acid 7f (3.12 g, 5.95 mmol) in dry DMF (20 mL) was treated with Hϋnigs base ( 3.07 g, 24.0 mmol, 4.0 equiv, 4.4 mL) and HOBt ( 1.2 g, 8.93 mmol, 1.5 equiv). The reaction mixture was cooled to 0°C and the treated with EDCI (1.35 g, 7.43 mmol, 1.25 equiv) and stirred for 1h. To this reaction mixture was added amine hydrochloride 1g (2.65g, 7.14 mmol, 1.2 equiv) was added and the reaction mixture was stirred at rt for 12 h. The DMF was distilled out and the residue was diluted with water and the aqueous layer was extracted with CH2CI2. The combined organic layers were extracted with aq NaHCO3, aq. HCl, brine, dried (Na2SO4) filtered concentrated in vacuo and the crude product 7g (4.3 g) was used for further cyclization without purification. H NMR (d4- CD3OD, 400 MHz, δ, ppm) 7.35 (t, 1 H), 6.72-6.60 (m, 5 H), 6.33-6.20 (dd, 2 H), 5.51 (s, 5 H), 4.19 (d, 1 H), 3.68 (s, 3 H), 3.19-2.83 (m, 2 H), 2.51-2.40 (m, 2 H), 2.40-2.25 (m, 2 H), 1.99-1.59 (m, 8 H), 1.35-0.98 (m, 5 H); MS (FAB, NBA-G/TG- DMSO, m/z relative intensity) 695.3 ([M-PFβ] > 100), 232 (20), 171 (30); HRMS calcd for C34H42N2O5CIRU " (M-PFβ) 695.1832; found 695.1845. Step G: 7g 7h A solution of chloro-compound 7g (3.0 g 3.6 mmol) in dry DMF (300 mL) was degassed with dry N2 and CS2CO3 ( 5.2 g, 16 mmol, 4.0 equiv) and stirred at rt. for 16h. The solvent DMF was distilled off and the residue was diluted with water and extracted with CH2CI2 (3x100 mL). The combined organic layers were dried (Na2S04) filtered, concentrated in vacuo and dried in vacuum overnight. It was used for photolytic removal of Ru without further purification. MS FAB (NBA- G/TG-DMSO 695 ([M-PF6] + , 100]. The cyclized compound from the previous step was dissolved in CH3CN (35mL) and photolysed in a Raynot (λ=350 nm) for 48 h. The reaction mixture was concentrated in vacuo and the residue was purified by chromatography (Siθ2, EtOAc/Hexanes 1 :1) to yield a tan colored solid 7h (600 mg, 34%). 1 H NMR (CDCI3, 400 MHz, δ, ppm) 7.58 (d, 1 H, J=7.6 Hz), 7.14 (t, 1 H, J=8.0 Hz), 6.94 (d, 2 H, J=8.4 Hz), 6.87 (dd, 1 H, J=2.4, 5.6 Hz), 6.73 (d, 1 H,.J=7.2 Hz), 6.59 (s, 1 H), 6.57 (s, 2 H), 6.39 (d, 1 H, J=8.0 Hz), 4.51 (dt, 1 H, J=2.8, 8.0 Hz), 3.80-3.62 (m, 1 H), 3.62 (s, 3 H), 3.05-3.00 (dd, 1 H, J=2.8, 11.6 Hz), 2.85 (dd, 1 H, J=8.4, 6.0 Hz), 2.76-2.72 (m, 1 H), 2.36-2.19 (m, 3 H), 2.02 (dd, 1 H, J=6.4, 9.2 Hz), 1.8- 1.73 (m, 1 H), 1.61-1.34(m, 7H), ,1.41-0.71 (m, 7 H). MS (FAB, NBA-G/TG- DMSO, m/z relative intensity), 493 [(M+1 ) + , 100], 465 (20), 232 (30), 171 (40); HRMS calcd. for C29H37N2O5 (M+1 ) + : 493.2702; found; 493.2699. Step H: 7h 7i A solution of ether 7h (220 mg, 0.46 mmol) in CH3OH (3.0 mL), CH2CI2 (10 mL) and H2O (0.5 mL) was treated with LiOH*H2θ (18 mg, 0.44 mmol, 1.1 equiv.) and stirred at rt for 12 h. The reaction mixture was acidified with aq.HCI (13 M, 1 mL) and concentrated in vacuo to yield acid 7i which was used directly for the coupling without further purification. Step I: A solution of acid 7i in dry DMF (3.0 mL) was treated with HOOBt (94 mg, 0.75 mmol, 1.6 equiv.), Hϋnigs base (237 mg, 1.84 mmol, 4.0 equiv.) and amine B (246 mg, 0.58 mmol, 1.47 equiv.) The reaction mixture was cooled to 0° C and treated with EDCI (110 mg, 0.58 mmol, 1.25 equiv) and stirred at 0 °C for 25 min for 12 h. The reaction mixture was concentrated in vacuo and diluted with H2O (30 mL). The combined aqueous layers were extracted with CH2CI 2 (3x30 mL). The organic layers were extracted with aq. HCl (1 M, 60 mL) aq. NaOH (60 mL), dried (Na2S0 ) filtered concentrated in vacuo to obtain a colorless solid 7j (230 mg) which was used for oxidation. MS: (Electron spray, m/z rel int): 854 [(M+1 ) , 100], 479 (70), 327 (50), 271.1 (100). Step J: A solution of alcohol 7j (220 mg, 0.26 mmol) in CH2CI2 (3.0 mL) was treated with Dess-Martin reagent (218 mg, 0.51 mmol, 2.0 equiv.) The reaction mixture was stirred at rt and the mixture was concentrated in vacuo. The residue was purified by chromatography (Siθ2, CH3OH/CH2CI2 1 :24) to yield oxidized product 7 (23 mg, 13%) as a colorless solid. MS: (FAB, m/z, rel. int.) 852 [(M+1 ) + , 43), 796 (100), 768 (20), 461 (20), 433 (50), 405 (50), 336 (30), 294, (50). Example 8: Preparation of Compound of Formula 8: Step A: A solution of tert-butyl ester 7 (32.0 mg, 37.0 μmol) was treated with TFA/CH2CI2 (1 :1 , 5.0 mL) and stirred at rt for 4 h. The disappearance of the ester to the base line was followed by TLC (CH3OH/CH2CI2 1 :24). After the deprotection was complete the reaction mixture was concentrated in vacuo and the residue was repeatedly treated with heptanes/CH3OH (4.0 mL) and concentrated to yield a tan solid 8 (29.0 mg, 100%). MS: (Electron spray, m/z rel int): 796 [(M+1 ) + , 100). Example 9: Preparation of Compound of Formula 9: Step A: A solution of Boc-Glycine 9a (1.75g,10.0 mmol) in dry DMF (50 mL) was treated with HOOBt ( 2.65 g, 15 mmol, 1.5 equiv) and EDCI (2.86g, 15.0 mmol, 1.5 equiv). The reaction mixture was treated with Hϋnigs base (5.16 g, 40 mmol, 4.0 equiv. 7.3 mL). The reaction mixture was stirred for 1 h and mete-tyrosine- OCH3*HCI 1e (2.5 g, 11.5 mmol, 1.1 equiv.) was added and stirred at 25 °C for 12 h. The reaction mixture was concentrated in vacuo and the residue was diluted with aq. NaHCO3 and extracted into CH2CI2. The combined organic layer were concentrated and the residue purified by chromatography (Siθ2, EtOAc/Hexanes 1 :1) to yield a colorless solid 9b (3.4g, 90%). Step B: 9b 9c A solution of 9b (4.6g, 13.06 mmol) in HCl (4M solution in Dioxane, 50 mL) was stirred at rt. for 3h. The reaction mixture was concentrated in vacuo and the residue was dried in high vacuum to yield 9c as a fine powder which was used for the next step. 1 H NMR (CD3OD, 400 MHz, δ, ppm) 8.67 (d, 1 H, J=7.9 Hz),7.10- 7.07 (m, 1 H), 6.68-6.64 (m, 2 H), 4.75-4.70 (m, 1 H), 3.75-3.61 (m, 2 H), 3.66 (s, 3 H), 3.10 (dd, 1 H, J= 5.2, 8.5 Hz), 2.90 (dd, 1 H, J=8.8 Hz, 5.0 Hz) . Step C: A solution of [CpRu(η -4-chlorophenyIpropionic acid)]PF6 1c (3.0 g, 46.01 mmol) in dry DMF (60 mL) was treated with HOBt (1.3 g, 9.16 mmol, 1.5 equiv.) and Hϋnigs base (3.22 g, 4.60 mL, 25.0 mmol, 4.0 equiv.) The reaction mixture was cooled to 0 ° C and treated with EDCI (1.75 g, 9.16 mmol, 1.5 equiv.) The reaction mixture was stirred at 0° C for 30 min and the glycine ammonium salt 9c (1.75 g, 6.06 mmol, 1.0 equiv.) was added. The reaction mixture was stirred at rt for 12 and the DMF was distilled out in vacuo. The residue was diluted with aq. HCl (1 M, 100 mL) and extracted into CH2CI2 (3x100 mL). The combined organic layers were extracted with aq. NaHCO3 (1x100 mL), brine (50 mL), dried (Na2SO4), filtered, concentrated in vacuo to yield a brown solid 9d (1.5 g, 34%) which was used for cyclization. MS: (Electron spray, m/z relative intensity): 585 [(M-PF6) + , 100], 459 (30), 373 (30), 198 (20). Step D: 9d 9e A solution of the ruthenium complex 9d (1.5 g 2.05 mmol) in dry DMF (100 mL) was degassed with dry N2 at rt. and CS2CO3 (5.0 g, 15 mmol, 7.5 equiv) was added and stirred at rt. for 12h. The solvent DMF was distilled off and the residue was diluted with water (100 mL) and extracted with CH2CI2 (3x100 mL) . The combined organic layers were extracted with brine (100 mL), dried (Na2SO4), filtered, concentrated in vacuo and dried in vacuum overnight. It was used for photolytic removal of Ru without further purification. The cyclized compound from the previous step was dissolved in CH3CN (30 mL) and filtered into a quartz tube. The solution was degassed and photolyzed in a Raynot (λ=350 nm) for 48h. The reaction mixture was concentrated in vacuo and the residue was purified by chromatography (Siθ2, EtOAc) to yield a tan colored solid 9e (230 mg, 30%). 1 H NMR (CDCI3, 400 MHz, δ, ppm) 7.23-7.18 (m, 2 H), 7.09-7.01 (m, 3 H), 6.76 (dd, 1 H, J=2.4, 8.8 Hz), 6.66 (d, 1 H, J=7.6 Hz), 6.47 (d, 1 H, J=5.6 Hz), 6.17 (s, 1 H), 5.64 (s, 1 H), 4.69 (q, 1 H, J=4.4 Hz), 3.77 (s, 3 H), 3.68-3.51 (m, 2 H), 3.35 (dd, 1 H, J=4.0, 10.8 Hz), 3.05 (dd, 1 H, J=5.2, 9.2 Hz), 2.96-2.92 (m, 2 H), 2.61-2.56 (m, 1 H), 2.30-2.29 (m, 1 H); 13 C NMR : (CDCI3, 100 MHz, δ ppm) 172.3, 171.4, 168.1 , 159.9, 155.4, 137.6, 136.4, 131.0, 130.0, 129.5, 123.3, 122.4, 121.0, 117.7, 117.1 , 53.6, 53.0, 43.6, 39.9, 36.1 , 32.3. MS: (Electron spray, m/z relative intensity): 383 [(M+1 ) + , 100], 279 (20) Step E: A solution of cyclic compound 9e (150 mg, 0.4 mmol) in THF (4.0 mL), H2O (4.0 mL) was stirred at rt with LiOH»H2θ (41.0 mg, 1.0 mmol, 2.5 equiv) for 3 h. The reaction mixture was acidified with cone. HCl (2.0 mL) and concentrated in vacuo. The solid 9f was dried in vacuo and used for further coupling without further purification. Step F: 9f B 9g A solution of the hydrolyzed acid 9f in dry DMF (4.0 mL) and CH2CI2 (4.0 mL) was treated with HOOBt (103mg, 0.58 mmol, 1.5 equiv) and cooled to 0 °C and Hϋnigs base (206 mg, 1.60 mmol, 4.0 equiv, 295 μL) was added. To this mixture was added EDCI (112 mg, 0.58 mmol, 1.5 equiv) and the reaction mixture was stirred at 0° C for 0.5 h and treated with the amine hydrochloride B (206 mg, 0.48 mmol, 1.2 equiv.). The reaction mixture was stored in freezer for 48 h and concentrated in vacuo to remove DMF and CH2CI2. The residue was diluted with aq. HCl (2M) and extracted with CH2CI2 (3x50 mL) The combined organic layer was extracted with aq. HCl (1 M, 3x50 mL), aq. NaOH (2M) brine (100 mL), dried (Na2SO4) and concentrated in vacuo. The residue 9g (200 mg) was oxidized without further purification. Step G: A solution of alcohol 9g (200 mg, 0.27 mmol) in CH2CI2 (3.0 mL) was treated with Dess-Martin reagent (342 mg, 0.81 mmol, 3.0 equiv.). The reaction mixture was stirred at rt for 3 h and diluted with aq. NaHCO3 and aq. Na2S2θ3. The reaction mixture was stirred at rt for 20 min and the reaction mixture was extracted with CH2CI2 (3x30 mL). The combined organic layers were extracted with brine (50 mL), dried (Na2SO4), filtered concentrated in vacuo and the residue was purified by chromatography (Siθ2, CH3OH (2M NH3)/CH2Cl2 1 :19) to yield ketoamide 9 (100 mg, 50%) of a colorless solid. MS: (Electron spray, m/z relative intensity): 742 ([M+1] + , 100), 686 (80). Example 10: Preparation of Compound of Formula 10: 10 Step A: 10 A solution of tert-butyl ester 9 (100 mg, 0.13 mmol) in dry CH2CI2 (4.0 mL) was treated with TFA (4.0 mL) and stirred at rt. for 5 h. The reaction mixture was concentrated in vacuo and the residue was repeatedly dissolved in toluene/CH2Cl2 and concentrated in vacuo several times to yield a fine colorless solid 10. MS (FAB, NBA/DMSO, m/z relative intensity), 686 [(M+1 ) + , 40], 460 (20), 307 (100), 289 (60); HRMS calcd. for C36H40N5O9 (M+1 ) + : 686.2825; found: 686.2840. Example 11 : Preparation of Compound of Formula 11 : Step A: 1 9 11a To a solution of amine hydrochloride 1g (1.20g, 3.23 mmol), 6-heptenoic acid (0.610 g, 4.68 mmol), HOOBt (0.765 g, 4.69 mmol) and EDCI (1.07 g, 5.58 mmol) in anhydrous DMF (50 mL) and CH2CI2 (50 mL) at -20°C was added NMM (1.55 mL, 14.1 mmol). After stirring at this temperature for 30 min, the reaction mixture was kept in a freezer for 18 h. It was then allowed to warm to rt. EtOAc ( 150 mL), brine (50 mL) and 5% H3PO4 (50 mL) were added. After separation, the organic solution was washed with 5% H3PO4 (80 mL), saturated aqueous sodium bicarbonate solution (2 X 80 mL), water (80 mL), and brine (80 mL), dried with magnesium sulfate, filtered and concentrated in vacuo. Flash chromatography (2 to 5 % MeOH-CH2Cl2) afforded 11a (1.46 g, 3.28 mmol, quant.) as a white solid. 1 H NMR (400 MHz, d6-DMSO) δ 9.25 (s, 1 H), 8.31 (d, J =7.2 Hz, 1 H), 7.70 (d, J =9.2 Hz, 1 H), 7.05-7.01 (m, 1 H), 6.62-6.58 (m, 3 H), 5.82-5.72 (m, 1 H), 5.02- 4.91 (m, 1 H), 4.43-4.38 (m, 1 H), 4.23-4.19 (m, 1 H), 3.55 (s, 3 H), 2.93-2.80 (m,2 H), 2.51-1.97 (m, 2 H), 1.66-0.86 (m, 15H); 13 C NMR (dβ-DMSO, 125 MHz), δ 171.9, 171.8, 171.1 , 157.2, 138.6, 138.4, 129.1 , 119.5, 115.8, 114.6, 113.5, 56.5, 53.5, 51.6, 36.5, 34.8, 32.8, 29.0, 28.0, 27.8, 25.8, 25.5, 24.8; HRMS, m/z 445.2683 (calcd for C-25H36N2O5: 445.2702, error: 4 ppm). Step B: 11a 11b To the solution of 11 a (1.46 g, 3.28 mmol) in anhydrous THF (60 mL) under nitrogen at 0°C was added borane-THF solution (12 mL, 1.0 M, 12 mmol) cautiously. The resulting solution was stirred at 0°C under nitrogen for 1 h 40 min. Then ethanol (4 mL) and pH 7 buffer (8 mL) were added, followed by aqueous 30 % H2O2 solution (7.5 mL). After stirred at 0°C for 20 min, it was warmed to rt and stirred for 2 h. EtOAc (200 mL) and brine (100 mL) were added and layers were separated. Aqueous solution was extracted with EtOAc (2 X 150 mL). Combined organic solution was dried with magnesium sulfate, filtrated, concentrated in vacuo. Flash chromatography (2 to 5 % MeOH-CH2Cl2) afforded 11b (1.05 g, 2.18 mmol, 68 %) as a white solid. H NMR (400 MHz, d6-DMSO) δ 9.25 (s, 1 H), 8.30 (d, J =7.2 Hz, 1 H), 7.68 (d, J =9.2 Hz, 1 H), 7.05-7.01 (m, 1 H), 6.62-6.58 (m, 3 H), 4.43-4.18 (m, 3 H), 3.55 (s, 3 H), 3.37-3.33 (m,2 H), 2.93-2.80 (m, 2 H), 2.20-2.03 (m, 2 H), 1.66-0.87 (m, 19H); 13 C NMR (d6-DMSO, 125 MHz), d 172.1 , 171.8, 171.2, 157.2, 138.4, 129.1 , 119.5, 115.8, 113.5, 60.7, 56.5, 53.5, 51.7, 36.5, 35.1 , 32.6, 32.4, 29.0, 28.5, 28.0, 25.8, 25.6, 25.4, 25.2; HRMS, m/z 463.2813 (calcd for C25H36N2O5: 463.2808, error: 1 ppm). Step C: 11b 11c To a solution of phenol alcohol 11b (1.00 g, 2.16 mmol) and tri-π- butylphosphine (1.10 mL, 4.28 mmol) in anhydrous CH2CI2 (100 mL) and THF (40 mL) at 0°C was added ADDP (1.08 g, 4.28 mmol). After stirring at 0°C for 1 h, the solution was warmed to rt and stirred for 3 h under nitrogen. TLC indicated complete consumption of the starting material. After removal of solvent in vacuo, the residue was partially purified by flash chromatography (0 to 3 % MeOH in CH2CI2) to afford the macrocycle 11c (650 mg, 1.46 mmol, 68 %). 1 H NMR (400 MHz, d6-DMSO) δ 8.58 (d, J =8.3 Hz, 1 H), 7.76 (d, J =9.2 Hz, 1 H), 7.18-7.14 (m, 1 H), 6.76-6.65 (m, 3 H), 4.77-4.71 (m, 1 H), 4.32 (t, J = 8.5 Hz, 1 H), 3.97-3.93 (m, 1 H), 3.82-3.78 (m, 1 H), 3.67 (s, 3 H), 3.18-3.14 (m, 1 H), 2.98-2.92 (m, 2 H), 2.32-2.25 (m, 1 H), 2.02-2.01 (m, 1 H), 1.99-0.87 (m, 19H); 13 C NMR (d6-DMSO, 125 MHz), δ 172.1 , 171.6, 171.4, 160.1 , 158.8, 139.0, 129.1 , 121.1 , 113.0, 111.9, 66.4, 56.1 , 52.0, 50.1 , 40.6, 34.9, 34.2, 28.7, 28.3, 26.8, 26.3, 25.9, 25.5, 25.2, 24.2; HRMS, m/z 445.2685 (calcd for C25H36N2O5: 445.2702, error: 4 ppm). Step D: 11c 11d Aqueous lithium hydroxide solution (70 mg, 15 mL H2O, 2.92 mmol) was added to a solution of methyl ester 11c (330 mg, 0.742 mmol) in THF (20 mL) and ethanol (10 mL) at rt. The mixture was stirred at rt for 3 h. The progress of the reaction was monitored by TLC. After the solution was concentrated in vacuo, EtOAc (100 mL), 6 N HCl solution (10 mL) and water (50 mL) were added and the layers were separated. The aqueous solution was extracted with EtOAc (2 X 80 mL). Organic solutions were combined, dried with magnesium sulfate, filtered and concentrated in vacuo to afford 11d (260 mg, 0.604 mmol, 81 %) as a white solid. 1 H NMR (400 MHz, d6-DMSO) δ 8.43 (d, J =8.3 Hz, 1 H), 7.73 (d, J =9.3 Hz, 1 H), 7.17-7.13 (m, 1 H), 6.77-6.66 (m, 3 H), 4.67-4.62 (m, 1 H), 4.32-4.28 (m, 1 H), 3.98-3.93 (m, 1 H), 3.81-3.75 (m, 1 H), 3.17-3.13 (m, 1 H), 2.97-2.90 (m, 1 H), 2.32-2.26 (m, 1 H), 2.01-1.97 (m, 1 H), 1.67-0.85 (m, 19H); 13 C NMR (d6-DMSO, 125 MHz), δ 173.2, 171.6, 171.3, 158.8, 139.3, 129.0, 121.1 , 113.1 , 111.9, 66.4, 56.1 , 50.8, 35.1 , 34.3, 28.8, 28.3, 26.9, 26.3, 25.9, 25.6, 25.5, 25.2, 24.2; HRMS, m/z 431.2564 (calcd for C-25H36N2O5: 431.2546, error: 4 ppm). Step E: 11d B 11e To a solution of acid 11d (0.140 g, 0.325 mmol), amine B (0.140 g, 0.325 mmol), HOOBt (56 mg, 0.343 mmol) and EDCI (75 mg, 0.391 mmol) in anhydrous DMF (40 mL) and CH2CI2 (20 mL) at -20°C was added NMM (0.107 mL, 0.973 mmol). After stirred at this temperature for 30 min, the reaction mixture was kept in a freezer for 18 h. Then EtOAc, brine and 5% H3PO4 were added. The separated organic solution was washed, successively, with 5% H3PO4, saturated aqueous sodium bicarbonate solution, water, and brine, dried with magnesium sulfate, filtered and concentrated in vacuo. Flash chromatography (2 to 5 % MeOH-CH2Cl2) afforded 11e as a mixture of diastereomers (0.170 g, 0.211 mmol, 65%) as a white solid, which is used in the next reaction without further purification. Step F: 11e 11 To the mixture of hydoxy amide 11e (0.29 g, 0.36 mmol) and Dess-Martin reagent (0.45 g, 1.06 mmol) at rt was added anhydrous CH2CI2 (60 mL), DMF (3 mL) and DMSO (3 mL). The resulting solution was vigorously stirred at rt for 2.5 h. More Dess-Martin reagent (300 mg, 0.71 mmol) was added and the reaction mixture was stirred for another hour. Saturated aqueous sodium bicarbonate and sodium bisulfite solutions (40 mL each) were added and the mixture was vigorously stirred for 10 min before EtOAc (200 mL) and water (30 mL) were added and the layers were separated. The organic solution was washed with 5% H3PO4 solution (2 X 100 mL) and saturated NaHCO3 solution (100 mL), dried with magnesium sulfate, filtered and concentrated in vacuo. Flash chromatography (1 to 5 % MeOH-CH2Cl2) afforded 11 (100 mg, 0.124 mmol, 35%) as a white solid. 1 H NMR (400 MHz, d6-DMSO) δ 8.79-8.69 (m, 2 H), 8.36- 8.16 (m, 2 H), 7.72-7.68 (m, 1 H), 7.42-7.33 (m, 5 H), 7.17-7.13 (m, 1 H), 6.77- 6.63 (m, 3 H), 5.30-5.27 (m, 1 H), 5.09-5.04 (m, 1 H), 4.85-4.76 (m, 1 H), 4.29- 4.25 (m, 1 H), 3.98-3.74 (m, 1 H), 3.02-2.85 (m, 2 H), 2.32-2.27 (m, 1 H), 2.04- 1.96 (m, 1 H), 1.72-0.81 (m, 35 H); 13 C NMR (d6-DMSO, 125 MHz), δ 196.5, 196.2, 171.65, 171.61 , 171.5, 171.14, 171.07, 169.4, 167.6, 160.7, 158.84, 158.79, 139.5, 139.3, 136.6, 136.5, 128.92, 128.90, 128.7, 128.6, 128.1 , 127.7, 127.4, 124.9, 121.34, 121.28, 113.1 , 112.9, 112.0, 111.9, 81.3, 66.34, 66.30, 56.92, 56.87, 56.3, 56.2, 53.4, 53.3, 51.5, 50.9, 41.5, 41.4, 40.8, 40.7, 36.6, 36.1 , 34.4, 34.3, 31.8, 31.6, 30.4, 29.1 , 28.9, 28.4, 28.3, 27.5, 26.8, 26.21 , 26.17, 25.9, 25.59, 25.55, 25.0, 24.2, 18.74, 18.66, 13.5, 13.4 ; HRMS, m/z 804.4542 (calcd for C-25H36N2O5: 804.4548, error: 1 ppm). Example 12: Preparation of Compound of Formula 12: 12 Step A: 11 12 A solution of the f-Butyl ester 11 (56.8 mg, 0.0706 mmol) in trifluoroacetic acid (15 mL) and CH2CI2 (15 mL) was stirred at rt for 4 h. After the volatiles were removed in vacuo, the residue was dissolved in 50% MeOH-CH2Cl2 (3 mL), and concentrated to dryness in vacuo to afford an off-white solid 12 (50 mg, 0.0669 mmol, 95%). H NMR (400 MHz, d6-DMSO) δ 8.75-8.71 (m, 2 H), 8.36-8.16 (m, 2 H), 7.72-7.69 (m, 1 H), 7.39-7.31 (m, 5 H), 7.17-7.13 (m, 1 H), 6.76-6.63 (m, 3 H), 5.37-5.35 (m, 1 H), 5.07-5.04 (m, 1 H), 4.85-4.76 (m, 1 H), 4.29-4.25 (m, 1 H), 3.97-3.74 (m, 4 H), 3.02-2.86 (m, 2 H), 2.32-2.26 (m, 1 H), 2.01-1.97 (m, 1 H), 1.70-0.82 (m, 26 H); 13 C NMR (d6-DMSO, 125 MHz), δ 196.5, 196.2, 171.63, 171.59, 171.52, 171.48, 171.1 , 171.06, 167.4, 160.6, 158.82, 158.78, 153.4, 139.4, 137.1 , 137.0128.91 , 128.88, 128.7, 128.65, 128.61 , 128.5, 128.43, 128.39, 128.33, 128.32, 128.14, 128.12, 128.0, 127.7, 128.7, 127.63, 127.59, 127.5, 127.4, 126.8, 121.3, 115.9, 113.1 , 112.9, 112.8, 112.0, 111.9,, 111.88, 66.33, 66.29, 56.3, 56.2, 56.17, 53.34, 53.31 , 53.27, 51.1 , 50.9, 41.5, 40.84, 40.77, 40.7, 40.6, 40.56, 40.53, 40.5, 38.7, 38.6, 38.56, 38.53, 36.6, 36.1 , 34.4, 34.3, 31.8, 31.6, 29.4, 29.1 , 29.0, e28.9, 28.4, 28.3, 28.2, 26.9, 26.8, 26.79, 26.20, 26.16, 25.88, 25.86, 25.79, 25.75, 25.71 , 25.66, 25.57, 25.54, 25.4, 25.0, 24.2, 18.7, 18.6, 13.5, 13.4; HRMS, m/z 748.3947 (calcd for C25H36N2O5: 748.3922, error: 3 ppm). Example 13: Preparation of Compound of Formula 13: 13 Step A: ig 13a The desired compound 13a was prepared according to the method of Example 11 , Step A. except substituting 6-hydroxyhexanoic acid for 6-heptenoic acid (39%). Step B: 13a 13b The desired compound 13b was prepared from 13a according to the method of Example 11 , Step C in 74% yield. Step C: 13b 13c The desired macrocyclic acid 13c was prepared from its corresponding methyl ester 13b according to the method of Example 11 , Step D in 88 % yield as a white solid. Step D: 13d The desired compound 13d was prepared from 13c and B according to the method of Example 11 , Step E in 48% yield. Step E: 13 The desired compound 13 was prepared from 13d according to the method of Example 11 , Step F in 70% yield. Example 14: Preparation of Compound of Formula 14: 14 Step A: 13 14 The desired compound 14 was prepared from 13 according to the method of Example 12, Step A in quantitative yield. Example 15: Preparation of Compound of Formula 15: Step A: 15a 15b A solution of 3-iodo-phenylanaline 15a, (2.50 g, 8.59 mmol) and concentrated hydrochloric acid (2 mL, 24 mmol) in methanoi was heated to reflux for 18 h. Removal of solvents in vacuo afforded a white solid 15b, which was used in Step B without further purification. Step B: 15c The desired compound 15c was prepared in 84% yield from 15b according to the method of Example 11 , Step A. It was used in the next reaction without further purification. Step C: 15c 15d The desired compound 15d was prepared from 15c according to the method of Example 11 , Step A (quantitative). It was used in the next reaction without further purification. Step D: 15d 15e The desired compound 15e was prepared in 68% yield from 15d according to the method of Example 11 , Step A. It was used in the next reaction without further purification. Step E: 15e 5 f A solution of 15e (1.16 g, 2.04 mmol), triethylamine (2.90 mL, 20.6 mmol) in anhydrous acetonitrile (25 mL) and DMF (20 mL) in a thick walled tube was bubbled with Argon for 5 min. To this solution at rt was quickly added tetrakistriphenylphosphine palladium (0) (235 mg, 0.203 mmol). The tube was sealed with Teflon screw cap and heated to 85-90°C in an oil bath. After stirring for 3 h, it was cooled to rt, opened cautiously and poured onto EtOAc (100 mL). The solution was washed with 5% H3PO4 (4 X 50 mL) and water (50 mL). Organic layer was dried with magnesium sulfate, filtrated, concentrated in vacuo. Flash chromatography (1 to 4 % MeOH-CH2Cl2) afforded the macrocycle 15f (330 mg, 0.749 mmol, 37 %). Step F: 15f 15g The desired compound 15g was prepared quantitatively from 15f according to the method of Example 11 , Step P. It was used in the next reaction without further purification. Step G: 15g B 15h The desired compound 15h was prepared in 77 % yield from 15g according to the method of Example 11 , Step F. It was used in the next reaction without further purification. Step H: The desired compound 15 was prepared in 55 % yield from 15h according to the method of Example 1 , Step H. Example 16: Preparation of Compound of Formula 16: 16 Step A: 15 16 The desired compound 16 was prepared quantitatively from 15 according to the method of Example 12, Step A. Example 17: Preparation of Compound of Formula 17: 17 Step A: 15f 17a To the solution of 15f (150 mg, 0.340 mmol) in EtOH (10 mL) and EtOAc (5 mL) was added 10 % palladium on carbon (20 mg). The suspension was stirred under hydrogen for 8 h during which the reaction progress was monitored by TLC. After filtration through a celite pad, solvents were removed in vacuo to afford the product as a white solid 17a (150 mg, 0.339 mmol, quantitative). It was used in the next reaction without further purification. Step B: 17a 17b The desired compound 17b was prepared from 17a according to the method of Example 11 , Step P. It was used in the next reaction without further purification. Step C: 17c The desired compound 17c was prepared in 73 % yield (Steps B and C) from 17b according to the method of Example 11 , Step E. It was used in the next reaction without further purification. Step D: 17 The desired compound 17 was prepared in 46 % yield from 17c according to the method of Example 11 , Step F. Example 18: Preparation of Compound of Formula 18: 18 Step A: 17 18 The desired compound 18 was prepared quantitatively from 17 according to the method of Example 12, Step A. Example 19: Preparation of Compound of Formula 19: 19 Step A: g 19b The desired compound 19b was prepared in 64 % yield from 1g and 19a according to the method of Example 1 1 , Step C. Step B: 19b 19c The desired compound 19c was prepared from 19b according to the method of Example 1 , Step C. It was used in the next reaction without further purification. Step C: 19c 19d To a suspension of di-amine salt 19c (75 mg, 1.52 mmol) and carbonyl diimidazole (260 mg, 1.60 mmol) in acetonitrile (400 mL) at rt was added triethylamine (0.26 mL, 1.85 mmol). The mixture was stirred for 3 days. Solvent was removed in vacuo. The residue dissolved in EtOAc/THF (100/50 mL) and the solution was washed with 5% H3PO4, dried with magnesium sulfate, filtered, concentrated in vacuo. Flash chromatography (2 to 10 % MeOH-CH2Cl2) afforded 19d (290 mg, 0.651 mmol, 43 %) as a white solid. Step D: 19d 19e The desired compound 19e was prepared in 97 % yield from 19d according to the method of Example 11 , Step P. It was used in the next reaction without further purification. Step E: B 19e 19f The desired compound 19f was prepared in 66 % yield from 19e and B according to the method of Example 11 , Step E. Step F: 19f 19 The desired compound 19 was prepared in 66 % yield from 19f according to the method of Example 11 , Step F. Two products were partially separated by flash chromatography (0 to 5 % MeOH-CH2Cl2). Example 20: Preparation of Compound of Formula 20: 20 Step A: 20 The desired compound 20 was prepared from 19 according to the method of Example 12, Step A. Example 21 : Preparation of Compound of Formula 21 : Step A: 21a 21b A solution of 5-hexene-1 -ol 21 a (1 Og, 50 mmol) in diethylether (100 mL) was treated with triethylamine (10.1g, 100 mmol, 2.0 equiv) and cooled to 0° C. A solution of phosgene in benzene (20%, 100 mL, 20g, 200 mmol, 4.0 equiv.) was added dropwise and the reaction mixture was stirred at rt for 12h. The triethylamine hydrochloride separating out was filtered and the filtrate was concentrated in vacuo. The residue 21b was used directly for further studies without purification. Step B: 1f 21c A solution of 1f (8.0g, 18.43 mmol) in CH2CI2 (100 mL) was treated with triethylamine (2.43 g, 24.0 mmol, 1.3 equiv). The reaction mixture was cooled to - 78° C and allychloroformate (2.9g, 24 mmol, 1.3 equiv.) was added dropwise. The reaction mixture was stirred at rt for 12 h and the reaction mixture was diluted with H2O (100 mL) and aq. HCl (2M, 200 mL). The aq. layer was extracted with EtOAc (3x200 mL). The combined EtOAc layer were extracted with brine, dried (Na2SO4), filtered concentrate in vacuo and the residue 21c was directly used for Boc deprotection. 1 H NMR (CHCI3, 300 MHz, δ, ppm) 7.29 (t, 1 H, J- 6.0 Hz), 7.06-6.98 (m, 3 H), 6.41 (d, 1 H, J=5.4 Hz), 6.05-5.95 (m, 1 H), 5.42 (dd, 1 H, J=1.2, 13.2), 5.31 (dd, 1 H, J=1.2, 13.2), 5.10 (d, 1 H, J=6.6 Hz), 4.91-4.87 (q, 1 H), 4.74 (d, 1 H, J=4.5 Hz), 3.95-3.92 (m, 1 H), 3.70 (s, 3 H), 3.12 (d, 1 H J=4.2 Hz),1.81-1.51 ( m, 6H), 1.43 (s, 9 H), 1.21-0.91 (m, 6 H). Step C: 21c 21d A solution of 21c (1.5 g) in HCl (4M in dioxane, 100 mL) was stirred at rt for 3 h. The disappearance of the starting material was followed by TLC and once the starting material disappeared the reaction mixture was concentrated in vacuo and the residue 21 d was dried in pump. It was used for coupling without further purification. Step D: 21e A solution of the aminehydrochloride 21 d (4.0g, 8.9 mmol) in CH2CI2 (50 mL) was treated with triethylamine (2.73 g, 27 mmol, 3.0 equiv, 3.8 mL) and cooled to -78° C. A solution of chloroformate 21b (2.3g, 13.3 mmol, 1.5 equiv) in CH2CI2 (30 mL) was added dropwise. The reaction mixture was stirred overnight at rt and diluted with aq. HCl (1 M, 150 mL). The aqueous layer was extracted with EtOAc (3x100 mL). The combined ethyl acetate layers were extracted with H2O (100 mL), brine (100 mL), dried (Na2SO4) filtered concentrated in vacuo and the residue was purified by chromatography (Siθ2, EtOAc/Hexanes 3:7) to yield 21 e as colorless solid (5g, 80%). Step E A solution of alloc-protected compound 21 e (4.0 g, 7.2 mmol) in dry THF (60.0 mL) was treated with dimedione (2.01 g, 14.4 mmol, 2.0 equiv.), Pd(PPh3)4 (830 mg, 0.71 mmol, 10 mol%) at 0 °C and stirred at rt for 1 h. The reaction mixture was concentrated in vacuo and the residue was purified by chromatography (Siθ2, EtOAc/Hexanes, 3:7) to yield a deprotected alcohol 21 f as a colorless solid (2.7g, 79%) H NMR (COCI3, 300 MHz, δ ppm) 7.44 (bs, 1 H), 7.09 (s, 1 H, J=6.0 Hz), 6.75-6.72 (m, 2 H), 6.58-6.48 (m, 2 H), 5.81-5.71 (m, 1 H), 5.55 (d, 1 H, J=7.2 Hz, 4.98 (ddd, 1 H, J=1.5, 1.2, 9 Hz), 4.92 (dd, 1 H, J=4.5, 0.9 Hz), 4.88-4.83 (m, 1 H), 4.12-3.97 (m, 1 H), 3.71 (s, 3 H), 3.09-2.98 (m, 2 H), 2.08-2.03 (m, 2 H), 1.722-1.40 (m, 10 H), 1.24-0.94(m, 5 H); 1 ^C NMR (100 MHz, δ) 171.6, 157.3, 156.6, 138.3, 136.6, 129.8, 123.5, 120.6, 117.0,114.9, 114.6, 65.7, 60.1 , 53.2, 52.5, 40.4, 37.1 , 33.3, 29.6, 28.6, 28.3, 26.0, 25.9, 25.1 ;.CHN: calcd for C25H36N2O6: C=65.20% H=7.88% N=6.08%; Found: C=64.90% H=7.98% N= 6.01 %. Step F: A solution of alkene 21f (650 mg, 1.4 mmol) in anhydrous THF (5.2 mL) was cooled to 0°C and treated with BH3»THF (1 M soln in THF, 4.2 mL, 4.2 mmol, 3.0 equiv.) The reaction mixture was stirred at rt for 2 h and EtOH (2.0 mL) was added carefully with the evolution of hydrogen gas. After the H2 evolution was complete the reaction mixture was treated with pH 7 buffer and treated with aq. H2O2 (30%, 5.0 mL) at 0 °C. The ice bath was removed and the mixture was stirred at rt for 3-4 h. The reaction mixture was extracted with EtOAc (3x100 mL). The combined organic layers were extracted with H2O, brine, dried (MgSO4), filtered, concentrated in vacuo and the residue was purified by chromatography (Siθ2, EtOAc/Hexanes 3:7) to yield hydroborated product as a colorless solid 21 g (400 mg, 60%) [α] D 86.4 (c 0.3 CHCI3, 25 °C); ^ H NMR (CPCI 3 , 400 MHz, δ) 7.26 (s, 1 H), 7.08 (t, 1 H, J=5.7Hz), 6.83 (d, 1 H, J=6.0 Hz), 6.71 (dd, 1 H, J=1.2, 4.5 Hz), 6.57 (bs, 1 H), 6.54 (d, 1 H, J=5.7 Hz), 5.68 (d, 1 H, J=6.9 Hz), 4.85 (dq, 1 H, J=4.2, 1.8 Hz), 4.05-3.97 (m, 3 H), 3.69 (s, 3 H), 3.60 (t, 2 H, J=4.8 Hz), 3.08- 2.97 (m, 2 H), 1.77-1.53 (m, 10H), 1.42 1.25 (m, 4 H), 1.24-0.92 (m, 5 H); 13 C NMR (CPCI 3 , 100 MHz, δ) 171.8, 171.8, 157.6, 156.9, 136.9, 130.0, 120.8, 117.0, 114.8, 65.7, 62.7, 60.3, 53.3, 52.7, 40.5, 37.4, 32.5, 29.7, 29.0, 28.8, 26.2, 26.0, 25.6, 25.4 MS (FAB, NBA/PMSO, m/z, relative intensity) 479 ([M+1] + ,100), 296 (40), 196 (25), 156 (25), 136 (25), 112 (20). HRMS calcd. for C25H39N2O7 (M+1) + : 479.2760; found 479.2757. Step G: 21g 21h A solution of PPh3 (385 mg, 1 ,47 mmol, 1.75 equiv)) in CH2CI2 (10 mL) was treated with the compound 21 g (400 mg, 0.84 mmol) and cooled to 0° C. A solution of OEAP (220 mg, 1.26 mmol, 1.5 equiv.) in CH2CI2 (10 mL) was added dropwise and stirred at rt for 3 h. The reaction mixture was concentrated in vacuo and the residue was purified by chromatography (Siθ2, EtOAc/Hexanes 1 :9) to yield cyclic product 21 h as a colorless solid. (110 mg, 25%) Step H: 21i A solution of cyclic carbamate 21 h (200mg, 0.44 mmol) in dioxane (30 mL), CH3OH (20 mL) and CH2CI2 (20 mL) was treated with LiOH«H2θ (80 mg, 2.0 mmol, 4.5 equiv) and stirred at rt for 4 h. The reaction was concentrated in vacuo and diluted with HCl (4M soln in dioxane, 10 mL) The water was removed by lyophilizer to yield the crystalline acid 21 i directly used for coupling. Step I 21j A solution of the hydrolyzed acid 21 i (210 mg, 0.47 mmol) in dry OMF (5.0 mL) and CH2CI2 (5.0 mL) was treated with HOOBt (125 mg, 0.70 mmol, 1.5 equiv) and cooled to 0 °C and Hϋnigs base (258 mg, 2.0 mmol, 4.0 equiv, 369 μL) was added. To this mixture was added EPCI (134 mg, 0.70 mmol, 1.5 equiv) and the reaction mixture was stirred at 0° C for 0.5 h and treated with the amine hydrochloride B (253 mg, 0.58 mmol, 1.25 equiv.). The reaction mixture was stored in freezer for 24 h and concentrated in vacuo to remove DMF and CH2CI2. The residue was diluted with aq. HCl (2M, 30 mL) and extracted with CH2CI2 (3x50 mL) The combined organic layer was extracted with aq. HCl (2M, 30 mL), aq. NaOH (1 M) brine (2x50 mL), dried (MgSO4) and concentrated in vacuo. The residue 21j (220 mg) was oxidized without further purification. Step J: 21 A solution of alcohol 21j (220 mg, 0.26 mmol) in CH2CI2 (5.0 mL) was treated with Dess-Martin reagent (200 mg, 0.47 mmol, 1.8 equiv.). The reaction mixture was stirred at rt for 1 h and diluted with aq. NaHCO3 (15 mL) and aq. Na2S2θ3 (15 mL). The reaction mixture was stirred at rt for 20 min and the reaction mixture was extracted with CH2CI2 (3x30 mL). The combined organic layers were extracted with aq. Na2CO3, dried (Na2SO4), filtered, concentrated in vacuo and the residue was purified by chromatography (Siθ2, CH3OH (2M NH3)/CH2Cl2 1 '-20) to yield ketoamide 21 (60 mg, 27%) of a colorless solid. Example 22: Preparation of Compound of Formula 22: Step A: 22 A solution of ferf-butyl ester 21 (50 mg, 0.059 mmol) in dry CH2CI2 (2.0 mL) was treated with TFA (2.0 mL) and stirred at rt. for 4 h. The reaction mixture was concentrated in vacuo and the residue was repeatedly dissolved in heptanes/ CH2CI2 and concentrated in vacuo several times to yield a fine tan solid 22 (47 mg), which was dried in vacuo. Example 23: Preparation of Compound of Formula 23: 23 Step A: 23a A solution of acid 1d (255mg, 1.0 mmol) in DMF (2.0 mL) was treated with HOBt (202 mg, 1.5 equiv) and Hϋnigs base (517 mg, 4.0 mmol, 4.0 equiv, 738 μL). The reaction mixture was cooled to 0° C and treated with DCC (258 mg, 1.25 mmol, 1.25 equiv.) After stirring the mixture for 1 h Histidine-OCH3«2HCI 23a (242.0 mg, 1.0 mmol) was added and stirred at rt overnight. The reaction mixture was concentrated in vacuo and extracted in EtOAc (3x50 mL) and aq. NaHCO 3 (50 mL). The combined organic layer was concentrated in vacuo and the residue was purified by chromatography (Siθ2, CH3OH/CH2CI2 1 :19) to yield dipeptide 23b as a colorless solid (380 mg, 93%) 1 H NMR (dβ-DMSO, 400 MHz, δ, ppm) 8.17 (d, 1 H, J=7.2 Hz), 7.48 (s, 1 H), 6.77 (s, 1 H) 6.57 (bs, 1 H), 5.54 (d, 1 H, J=7.6 Hz), 4.47 (q, 1 H, J=7.2 Hz), 3.79 (t, 1 H, J=8.4 Hz), 3.55 (s, 3 H), 3.36-3.20 (m,2 H), 2.94-2.82 (m, 2 H), 1.70-1.47 (bm, 6H), 1.35 (s, 9 H), 1.46-0.85 (m, 5 H): 13 C NMR (d6-DMSO, 100 MHz, δ ppm) 172.5, 171.9, 157.3, 155.9, 135.4, 78.6, 59.5, 52.9, 52.3, 34.1 , 29.6, 28.9, 28.6, 26.5, 26.3, 26.0, 25.2 FAB MS: (NBA-G/TG-DMSO, m/z relative intensity) 409. [(M+1 ) + , 100], 353. (10), 170 (20); HRMS calcd for C-20H33N4O6: 409.2451 : found 409.2466; CHN Calcd for C-20H32N4O5: C=58.81 % H=7.90%, N=13.72%; Found: C=58.70% H=7.78% N=13.43% . Step B: A solution of ω-bromoheptenoic acid (223 mg, 1.0 mmol) in DMF (3.0 mL) was treated with deprotected amine hydrochloride 23b (380 mg, 1.0 mmol, 1.0 equiv) and Hϋnigs base (387 mg, 3.0 mmol, 3.0 equiv) was added. The reaction mixture was treated with PyBroP (465 mg, 1.0 mmol) and stirred at rt for 3 h. The reaction mixture was concentrated in vacuo and the residue was purified by chromatography (Siθ2, CH3OH/CH2CI2, 1 :19) to yield a colorless solid (220 mg, 50 %). MS (FAB) 515.2 [(M+1) + , 100], 513.2 [(M+1) + , 95)], 469 (60), 433 (20), 170 (40). HRMS calcd. for C23H38BrN4θ4: 513.2076 found: 513.2073. Step C: A solution of bromo-compound 23c (100 mg, 0.23 mmol), in 2-butanone (4.0 mL) was treated with Na2CO3 (31.0 mg, 0.29 mmol, 1.25 equiv) and with Lil (50 mg, 0.37 mmol 1.3 equiv.) and heated at reflux for 24 h. The reaction mixture was concentrated in vacuo and the residue was diluted with water. The residue was extracted with CH2CI2 (3x30 mL). The combined organic layer was dried( Na2SO4) and purified by chromatography (Siθ2, CH3OH:CH2Cl2 1 :19) to yield the cyclized compound 23d (25 mg, 31 %); Rf: 0.68 (2M NH 3 in CH 3 OH:CH 2 CI 2 : 1 :19) 1 H NMR (CDCI3, 400 MHz, δ, ppm) 8.17 (d, 1 H, J=8.8 Hz) 7.33 (s, 1 H), 6.48 (d, 1 H, J=8.4 Hz), 4.90-4.85 (m, 1 H), 4.26 (t, 1 H, J=8.0 Hz), 3.82-3.74 ( m, 2 H), 3.69 (s, 3H), 3.16- 3.11 (m, 2 H) 2.91-2.84 (m, 1 H), 2.30-2.01 (m, 2 H), 1.65-1.59 (m, 11 H), 1.18-0.96 (m, 11 H): 13 C NMR (CDCI3, 100 MHz, δ ppm): 172.8, 172.4, 171.9, 138.2, 136.8, 57.6, 52.5, 51.7, 46.6, 41.6, 36.0, 30.9, 29.5, 28.8, 27.3, 26.7, 26.4, 26.3, 26.2, 25.2, 24.8 MS: (Electron spray, m/z relative intensity): 433.1 [(M+1) + ,100]; HRMS: Calcd. for C23H37N4O4 433.2815 found 433.2822. Step D: 23d 23e A solution of methyl ester 23d (200 mg, 0.46 mmol) in CH3OH (5.0 mL) H2O (0.5 mL) was treated with LiOH«H2θ (30 mg, 0.75 mmol, 1.6 equiv.) The reaction mixture was stirred at rt for 15 h and concentrated in vacuo and dried in pump to yield hydrolyzed compound 23e which was used for coupling directly. Step E: 23f A solution of the acid 23e in CH2CI2 (3.0 mL), DMF (5.0 mL) was treated with HOOBt (115 mg, 0.70 mmol, 1.50 equiv) and EDCI (113 mg, 0.60 mmol, 1.25 equiv). The reaction mixture was then treated with Et3N (190 mg, 1.88 mmol, 271 μL, 4.0 equiv) and amine hydrochloride B (201 mg, 0.5 mmol, 1.1 equiv.) The reaction mixture was stirred at rt for 13 h and diluted with H2O. The aqueous layer was extracted with CH2CI2 (3x50 mL) and the combined organic layers were extracted with aq. NaOH (1 M, 50 mL) and dried (Na2SO4). The dried organic layer was filtered and concentrated in vacuo to yield a colorless residue 23f (442 mg) which was dried in vacuo and directly used for further oxidation. MS: (Electron spray, m/z relative intensity): 794 [(M+1) , 100]. Step F: A solution of hydroxy-amide 23f (50 mg, 0.064 mmol) in CH2CI2 (3.0 mL) was treated with Dess Martin reagent (53 mg, 0.13 mmol, 2.0 equiv) and stirred at rt for 3 h. The reaction mixture was diluted with aq. satd. Na2S2θ3 (20 mL) and stirred at rt for 15 min. The aqueous layer was extracted with CH2CI2 (3x30 mL). The organic layer was dried (Na2SO4) filtered concentrated in vacuo and purified by chromatography (Siθ2, CH3OH/CH2CI2, 1 :15) to yield ketoamide 23 (20mg, 40%); MS (FAB, NBA-G/TG-DMSO, m/z relative intensity) 824 [(M+CH3OH) + , 100], 792 [(M+1 ) + , 60], 447 (20); HRMS calcd for C42H62N7O8 (M+1 ) + : 792.4660: found 792.4659. Example 24: Preparation of Compound of Formula 24: Step A: A solution of tert-butyl ester 23 (17 mg, 21.5 μmol) in dry CH2CI2 (2.0 mL) was treated with TFA (2.0 mL) and stirred at rt. for 8 h. The disappearance of the ester to the base line was followed by TLC (CH3OH/CH2CI2, 1 :19). The reaction mixture was concentrated in vacuo and the residue was repeatedly dissolved in CH3OH/heptanes/CH2Cl2 and concentrated in vacuo several times to yield 24 as fine colorless solid (7 mg). MS: (Electron spray, m/z relative intensity): 768 [(M+CH3OH) + , 100], 736 [(M+1 ) + , 60], 46 (10). Example 25: Preparation of Compound of Formula 25: 25 Step A: 25a 1b 25b A solution of Boc-4-Chlorophenylalanine 25a (523 mg, 1.75 mmol) in dichloroethane (37 mL) was treated with CpRu(CH3CN)3 PF6 1b (760 mg, 1.75 mmol, 1.0 equiv) and heated at reflux for 2 h. The reaction mixture was cooled to 0 °C and filtered. The filtrate was concentrated in vacuo and dissolved in minimum CH3CN and treated with a large excess of Et2θ. The solid separating out was separated and dissolved in CH2CI2/CH3OH (1 :1 , 50 mL) and concentrated in vacuo to obtain 25b as a brown foam (640 mg, 69%). Step B: A solution of carboxylic acid 25b (2.4 g, 3.80 mmol) in dry DMF (15 mL) was treated with Hϋnigs base (1.64 g, 12.64 mmol, 4.0 equiv, 2.9 mL) and HOBt (661 mg, 4.38 mmol, 1.5 equiv). The reaction mixture was cooled to 0° C and the treated with EDCI (699 mg, 3.95 mmol, 1.25 equiv) and stirred for 15 min. To this reaction mixture was added amine hydrochloride 1g (1.50 g, 4.00 mmol, 1.2 equiv) and the reaction mixture was stirred at rt for 12 h. The DMF was distilled out and the residue was diluted with water (30 mL) and the aqueous layer was extracted with CH2CI2 (3x50 mL). The combined organic layers were extracted with aq NaHCθ3 (30 mL). aq- HCl (30 mL), brine, dried (Na2SO4) filtered concentrated in vacuo and the crude product 25c (2.5 g, 69%) was used for further cyclization without purification. Step C: 25c 25d A solution of compound 25c (100 mg 0.11 mmol) in dry DMF (10 mL) was degassed with dry N2 and treated with CS2CO3 (170 mg, 0.5 mmol, 5.0 equiv) and stirred at rt. for 12 h. The solvent DMF was distilled off and the residue was diluted with water (35 mL) and extracted with CH2CI2 (3x100 mL). The combined organic layers were dried (Na2S04) filtered, concentrated in vacuo and dried in vacuum overnight. It was used for photolytic removal of Ru without further purification. The cyclized compound from the previous step was dissolved in CH3CN and photolysed in a Raynot (λ=350 nm) for 48h. The reaction mixture was concentrated in vacuo and the residue was purified by chromatography (Siθ2, EtOAc/Hexanes 2:1) to yield 25d as a tan colored solid (29 mg, 46 %). MS (FAB, NBA-G/TG-DMSO, m/z relative intensity), 580 [(M+1)\ 80], 524 (100), 418 (40), 462 (30), 452 (20), 313 (60), 253 (20). Step D: 25d 25e A solution of ester 25d (150 mg, 0.26 mmol) in THF (3 mL), CH3OH (3.0 mL) and H2O (3.0 mL) was treated with LiOH*H2θ (18 mg, 0.43 mmol, 1.65 equiv.) and stirred at rt for 35 min. The reaction mixture was acidified with cone HCl (13 M, 1mL) and extracted in CH2CI2 (3x50 mL).The combined organic layers were dried (Na2S04) filtered and concentrated in vacuo to yield acid 25e which was used directly for the coupling without further purification. Step E: A solution of acid 25e (150 mg, 0.27 mmol) in dry CH2CI2 (2.0 mL), was treated with HOBt (62 mg, 0.40 mmol) and Hϋnigs base (139 mg, 1.1 mmol, 4.0 equiv.) The reaction mixture was cooled to 0° C and treated with EDCI (53 mg, 0.34 mmol, 1.25 equiv) and stirred for 30 min. The reaction mixture was treated with amine F (88 mg, 0.29 mmol, 1.22 equiv.) and stored in the freezer for 12 h. The reaction mixture was concentrated in vacuo and diluted with H2O (50 mL). The aqueous layer were extracted with CH2CI2 (3x50 mL). The combined organic layers were extracted with aq. HCl (1 M, 3x20 mL) aq. NaOH (1 M, 3x20 mL), dried (Na2SO4) filtered concentrated in vacuo to obtain a colorless solid 25f (138 mg) which was used for oxidation. Step F: 25 A solution of alcohol 25f (140 mg, 0.143 mmol) in CH2CI2 HF (1 :1 , 5.0 mL) was treated with Dess-Martin reagent (121 mg, 0.42 mmol, 3.0 equiv.) The reaction mixture was stirred at rt for 2 h and the mixture was concentrated in vacuo. The residue was purified by chromatography (Siθ2, CH3OH/ CH2CI2 1 :32) to yield oxidized product 25 (57mg, 41%) as a colorless solid. Example 26: Preparation of Compound of Formula 26: Step A: A solution of the benzyl ester 25 (30 mg, 38.0 mol) in CH3OH/THF (1 :1 , 4.0 mL) was treated with Pd/C (20 mg, 10% ) and H2 was bubbled through it. A drop of acetic acid was added to accelerate the reduction. The reaction mixture was filtered through a plug of celite and the filtrate was concentrated in vacuo. The residue 26 was analyzed without further purification. Example 27: Preparation of Compound of Formula 27: 27 Step A: 27a A solution of acid 25e(100 mg, 0.17 mmol) in dry CH2CI2 was treated with HOOBt (41 mg, 0.26 mmol) and Hϋnigs base (91 mg, 0.70 mmol, 4.0 equiv.) The reaction mixture was cooled to 0° C and treated with EDCI (35 mg, 0.22 mmol, 1.25 equiv) and stirred for 30 min. The reaction mixture was treated with amine D (71 mg, 0.22 mmol, 1.22 equiv.) and stored in the freezer for 12 h. The reaction mixture was concentrated in vacuo and diluted with H2O (30 L). The aqueous layers was extracted with CH2CI2 (3x30 mL). The organic layers were extracted with aq. HCl (1M, 30 mL) aq. Na2CO3 (1 M, 30 mL), dried (Na2SO4) filtered concentrated in vacuo to obtain 27a colorless solid (119 mg) which was used for oxidation. MS (FAB), 842 [(M+1), 100], 765 (20), 735 (10), 657 (20), 575 (10), 492 (10), 464 (20), 446 (30). HRMS calcd. for C46H6ON5O10 (M+1)+: 842.4339; found 842.4336. Step B: 27 A solution of alcohol 27a (120 mg, 0.143 mmol) in CH2CI2 HF (1 :1 , 3.0 mL) was treated with Dess-Martin reagent (180 mg, 0.42 mmol, 3.0 equiv.) The reaction mixture was stirred at rt. for 2 h and the mixture was concentrated in vacuo. The residue was purified by chromatography (Siθ2, CH3OH/CH2CI2 1 :32) to yield oxidized product 27 as a colorless solid. MS (FAB, NBA-G/TG-DMSO, m/z rel int), 840 [(M+1) + , 50]. HRMS calcd. for C46H58N5O10 (M+1)+: 840.4184; found 840.4199. Example 28: Preparation of Compound of Formula 28: Step A: 28 A solution of benzyl ester 27 (40 mg, 47.0 mol) in CH3OH/THF (1 :1 , 6.0 mL) was treated with Pd/C (30 mg, 10% ) and H2 was bubbled through it. A drop of acetic acid was added to accelerate the reduction. The reaction mixture was filtered through a plug of celite and the filtrate was concentrated in vacuo to yield 28. Example 29: Preparation of Compound of Formula 29: 29 Step A: 29a 29b A solution of 4-chlorophenylalanine 29a (1.5g, 7.5 mmol) in THF (20 mL) and H2O (20 mL) was treated with NaOH (900 mg, 22.5 mmol, 3.0 equiv.) and cooled to 0°C, A solution of acetyi chloride (707 mg, 9.00 mmol, 1.25 mmol) in THF (10 mL) was added dropwise and the reaction mixture was stirred overnight at rt. The reaction mixture was acidified with aq. HCl (1 M, 10 mL) and extracted with CH2CI2 (3x30 mL). The combined organic layers were dried (Na2SO4) filtered concentrated in vacuo to yield 29b which was used in the next step without purification. Step B: A solution of N-acetyl-4-chlorophenylalanine 29b (1.39 g, 5.75 mmol) in dichloroethane (118 mL) was treated with CpRu(CH3CN)3 PF6 1b (2.5 g, 5.8 mmol, 1.0 equiv.) and heated at reflux for 2 h. The reaction mixture was cooled to 0 °C and filtered. The filtrate was concentrated in vacuo and dissolved in CH3CN (15 mL) and treated with Et2θ (150 mL). The gum separating out was separated by decanting the ether and the residue was dissolved in CH2CI2/CH3OH (1 :1 , 50 mL) and concentrated in vacuo to obtain 29c as a brown foam (2.2 g, 69%). MS: (Electron spray, m/z rel int): 408 [(M-PFβ) + , 100]. Step C: A solution of carboxylic acid 29c (2.0 g, 4.00 mmol) in dry DMF (20 mL) was treated with Hϋnigs base (2.06 g, 16.0 mmol, 4.0 equiv., 2.9 mL) and HOBt (810 mg, 6.0 mmol, 1.5 equiv.). The reaction mixture was cooled to 0°C and then treated with EDCI (888 mg, 5.0 mmol, 1.25 equiv.) and stirred for 0.5 h. To this reaction mixture was added amine hydrochloride 1g (1.48 g, 7.14 mmol, 1.2 equiv.) and the reaction mixture was stirred at rt. for 12 h. The DMF was distilled out and the residue was diluted with water and the aqueous layer was extracted with CH2CI2 (3x100 mL). The combined organic layers were extracted with aq. NaHC03 (200 mL), aq- HCl (100 mL), brine, dried (Na2Sθ4) filtered concentrated in vacuo and the crude product 29d (1.2 g, 38%) was used for cyclization without further purification. Step D: 29d 29e A solution of chloro-compound 29d (1.2 g 1.5 mmol) in dry DMF (120 mL) was degassed with dry N2, treated with CS2CO3 (2.4 g, 7.4 mmol, 5.0 equiv.) and stirred at rt. for 23 h. The solvent DMF was distilled off and the residue was diluted with water (300 mL) and extracted with propionitrile (3x100 mL) '. The combined organic layers were dried (Na2Sθ4) filtered, concentrated in vacuo and dried in vacuum overnight. It was used for photolytic removal of Ru without further purification. The cyclized compound from the previous step was dissolved in CH3CN (40 mL) and photolyzed in a Raynot (λ=350 nm) for 48h. The reaction mixture was concentrated in vacuo and the residue was purified by chromatography (Siθ2, EtOAc/Hexanes 4:1) to yield a tan colored solid 29e (240mg, 38 %). MS (FAB, NBA-G/TG-DMSO, m/z relative intensity), 522[(M+1) + , 100]. Step E: 29e 29f A solution of ester 29e (200 mg, 0.42 mmol) in CH3OH (5 mL), CH2CI2 (13 L) and H2O (2.0 mL) was treated with UOH.H2O (41 mg, 1.0 mmol, 2.4 equiv.) and stirred at rt. for 3 h. The reaction mixture was acidified with aq. HCl (13 M, 1 mL) and extracted in CH2CI2 (3x50 mL) and EtOAc (3x50 mL) The combined organic layers were dried (Na2Sθ4) filtered and concentrated in vacuo to yield acid 29f (178 mg) which was used directly for the coupling without further purification. Step F: A solution of acid 29f (90 mg, 0.18 mmol) in dry DMF (1.0 mL) was treated with HOBt (45 mg, 0.33 mmol, 1.6 equiv.), Hϋnigs base (142 mg, 1.1 mmol, 5.0 equiv.) and amine B (118 mg, 0.28 mmol, 1.47 equiv.) The reaction mixture was cooled to 0° C and treated with EDCI (63 mg, 0.33 mmol, 1.6 equiv.) and stirred at 0 °C for 20 min. for 12 h. The reaction mixture was concentrated in vacuo and diluted with H2O (30 mL). The combined aqueous layers were extracted with CH2CI2 (3x30 mL) and EtOAc (3x30 mL). The organic layers were extracted with aq. NaOH (2M, 30 mL), dried (Na2S04) filtered concentrated in vacuo to obtain a colorless solid 29g (50 mg, 32%) which was used for oxidation. MS: (Electron spray, m/z rel int): 883 [(M+1V + 1" , 100], 522 (30), 394 (60). Step G: 29 A suspension of alcohol 29g (50 mg, 60.0 //mol) in CH2CI2 (2.0 mL) was treated with Dess-Martin reagent (40 mg, 0.94 mmol, 2.0 equiv.) The reaction mixture was stirred at rt. for 3h and the mixture was concentrated in vacuo. The residue was purified by chromatography (Siθ2, CH3OH/CH2CI2: 1 :32) to yield oxidized product 29 (41 mg, 80%) as a colorless solid. MS: (FAB, m/z, rel. int.) 881 [(M+1)+, 100), 825 (170), 248 (100). Example 30: Preparation of Compound of Formula 30: 30 A solution of tert-butyl ester 29 (23.0 mg, 26.0 /mol) was treated with TFA/CH2CI2 (1 :1 , 2.0 mL) and stirred at rt. for 4 h. The disappearance of the ester to the base line was followed by TLC (CH3OH/CH2CI2 1 :24). After the deprotection was complete the reaction mixture was concentrated in vacuo and the residue was repeatedly treated with heptanes/CH2Cl2 (4.0 mL) and concentrated to yield a tan solid 30 (13.0 mg, 100%). MS: (Electron spray, m/z rel int): 825 [(M+1)+, 100). Example 31 : Preparation of Compound of Formula 31 : 31 Step A: A solution of acid 29f (150 mg, 0.29 mmol) in dry DMF (4.0 mL), CH2CI2 (3.0 mL) was treated with HOBt (58 mg, 0.44 mmol) and Hϋnigs base (149 mg, 1.1 mmol, 4.0 equiv.) The reaction mixture was cooled to 0° C and treated with EDCI (82 mg, 0.44 mmol, 1.5 equiv.) and stirred for 30 min. The reaction mixture was treated with amine E (88 mg, 0.29 mmol, 1.22 equiv.) and stirred at rt. for 12 h. The reaction mixture was concentrated in vacuo and diluted with H2O (30 mL). The aqueous layer was extracted with CH2CI2 (3x30 mL). The combined organic layers were extracted with aq. HCl (1 M, 3x20 mL), aq. NaOH (1 M, 3x20 mL), dried (Na2S04) filtered concentrated in vacuo to obtain a colorless solid 31a (56 mg) which was used for oxidation. MS: (Electron spray, m/z rel int): 750 [(M+1) + , 20], 663 (10), 522 (10), 416 (20), 247 (30). Step B: 31 A solution of alcohol 31a (56 mg, 75 /mol) in CH2CI2 (5.0 mL) was treated with Dess-Martin reagent (93 mg, 0.22 mmol, 3.0 equiv.) The reaction mixture was stirred at rt. for 4 h and the mixture was concentrated in vacuo. The residue was purified by chromatography (Siθ2, CH3OH/CH2CI2: 1 :19) to yield oxidized product 31 (34 mg, 60 %) as a colorless solid. MS: (Electron spray, m/z rel int): 748 [(M+1) + , 35], 692 (5), 279 (100). Example 32: Preparation of Compound of Formula 32: Step A: 3 A solution of tert-butyl ester 31 was treated with TFA/CH2CI2 (1 :1 , 4.0 mL) and stirred at rt. for 4 h. The disappearance of the ester to the base line was followed by TLC (CH3OH/CH2CI2 1 :24). After the deprotection was complete the reaction mixture was concentrated in vacuo and the residue was repeatedly treated with heptanes/CH2θ2 (4.0 mL) and concentrated to yield 32 as a tan solid. Example 33: Preparation of Compound of Formula 33: 33 Step A: 33a 33b A solution of acid 33a (4.5g, 25.0 mmol) in dioxane (30 mL) and benzene (80 mL) was treated with BnOH (8.0 g, 74 mmol, 3.0 equiv.) and TsOH.H2θ (713 mg, 3.75 mmol, 10 mol%) The reaction mixture was heated at reflux for 5 h, when the water was separated using a Dean-Stark apparatus. The reaction mixture was concentrated in vacuo and the residue was purified by chromatography (Siθ2, EtOAc/Hexanes 3:7) to yield benzyl ester 33b as a colorless oil (4.2 g, 62%); Rf : 0.22 (EtOAc/Hexanes 3:7); 13 C NMR (CH3OD, 75 MHz, δ): 175.1 , 158.2, 139.7, 130.3, 129.5, 129.3, 121.7, 117.4, 114.6, 73.1 , 67.6, 41.6; MS (FAB, G/TG- DMSO, m/z, relative intensity): 351 ([M+DMSOf, 70), 273 ([M+1]+, 100), 255 (20), 227 (30), 181 (40); HRMS: Calcd. for C16H17O4 (M+1) + 272.1049; found 272.1054. Step B: 33b 33c A solution of benzyl ester 33b (3.8 g, 12.9 mmol) in CH2CI2 (100 mL) was treated with Et3N (1.55 g, 15.4 mmol, 2.2 mL, 1.1 equiv.), cooled to -78°C (2- PrOH, dry ice) and a solution of allyl chloroformate (1.84 g, 15.36 mmol, 1.1 equiv.) in CH2CI2 (10 mL) was added dropwise. The reaction mixture was allowed to warm up to the rt. and diluted with aq. HCl (1 M, 100 mL). The reaction mixture was extracted with EtOAc (3x100 mL). The combined organic layer was washed with aq. HCl (100 ml, 1 M), brine (100 mL), dried (MgSθ4) concentrated in vacuo to yield 33c which was used in the next step without further purification. Rf : 0.43 (EtOAc/Hex 7:13); 13 C NMR (CH3OD, 75 MHz, δ) 174.8, 162.5, 155.0, 152.5, 140.3, 137.1 , 132.8, 130.3, 129.6, 129.5, 129.4, 123.2, 120.3, 119.4, 72.7, 70.1 , 67.7, 41.2, 29.9. Step C: 33c 33d A solution of Boc-cyclohexylglycine monohydrate 1d (6.02 g, 23.4 mmol, 2.0 equiv.) was dissolved in CH2CI2 and dried (MgSθ4). The mixture was filtered and the residue was further azeotropically dried with toluene. The residue was dissolved in CH2CI2, and treated with HOBt (4.73 g, 35.1 mmol, 2.9 equiv.) EDCI (6.7 g, 35.1 mmol, 2.9 equiv.) and Hϋnigs base (8.31 g, 64.3 mmol, 11 mL) It was stirred at rt. for 30 min. and the alloc protected alcohol 33c (4.3 g, 12.04 mmol) was added. The reaction mixture was stirred at rt. for 36 h and diluted with aq. HCl (1 M, 100 mL) and extracted with EtOAc (3x100 mL). The combined organic layers were extracted with aq. NaOH (1 M, 100 mL), brine (100 mL), dried, concentrated in vacuo and purified by chromatography (Siθ2, EtOAc/Hex 1 :4) to yield depsipeptide 33d (7.1 g 100%). Rf : 0.18 (EtOAc/Hex 1 :4); HRMS: Calcd. for C28H34O7 (M-Boc) + 496.2335 : found 496.2333. Step D: 33d 33e A solution of alloc-protected depsipeptide 33d (7.8 g, 13.0 mmol) in dry THF (200 mL) was treated under N2 with dimedione (3.27 g, 23.4 mmol, 2.0 equiv.) and Pd(Ph3P)4 (780 mg, 0.67 mmol, 5 mol%). The reaction mixture was stirred at rt. for 1 h and the disappearance of reactant was followed by TLC (EtOAc/Hex 1 :4). The reaction mixture was concentrated in vacuo and the residue was purified by chromatography (Siθ2, EtOAc/Hexanes 1 :4) to yield phenol 33e (5.2g, 78%) as a colorless foam. Rf : 0.52 ( EtOAc/Hexanes 3:7); 1 H NMR (d4- CD3OD, 300 MHz, δ ) 7.4-7.19 ( m, 5 H), 7.15-6.99 (m, 1 H), 6.68-6.55 (m,4 H) 5.43-5.01 (m,3 H), 4.6 (bs, 2H), 4.11-4.00 (m, 1 H), 3.18-2.91 (m, 2 H), 1.80-1.55 (bs, 6H) 1.39 (s, 9 H) 1.21-0.89 (m, 6 H); 13 C NMR (CH3OD, 75 MHz, δ, mixture of diastereomers) 171.6, 169.4, 169.3, 161.1 , 157.1 , 157.0, 137.2, 136.9, 135.4, 135.3, 129.2, 129.1 , 128.2, 128.2, 128.0, 120.3, 120.1 , 116.0, 115.9, 113.6, 94.8, 79.3, 73.6, 73.5, 66.7, 66.6, 58.6, 58.5, 40.0, 39.9, 36.8, 29.1 , 27.7, 27.3, 25.5. MS (Electron spray, m/z, relative intensity) 1023 ([2M+1] + , 20), 512 ([M+1] + , 20), 412 ([M-Boc] + , 100), 202 (40) HRMS Calcd. for C24H30NO5 (M-Boc) + 412.2123 : found 412.2119. Step E: 33e 33f A solution of Boc protected amine 33e (5.2 g, 10.7 mmol) was stirred with HCl (4M, dioxane, 200 mL, 800 mmol, 80 equiv.) until the starting material disappeared to the base line as indicated by TLC (EtOAc/Hex 3:7). The reaction mixture was concentrated in vacuo and dried in high vacuum and the residue 33f was directly used in the next step. 1 H NMR (d4-CD30D, 300 MHz, δ ) 7.40-3.23 (m, 5 H), 7.07 (q, 1 H, J=13 Hz) 6.77-6.6 (m, 3 H), 5.33-5.41 (m, 1 H), 5.3-5.05 (2 AB, 2 H) 3.99-3.85 (m, 1 H) 3.35-22 (m, 2 H) 2.00-1.5 (m, 5 H), 1.50-0.80 (m, 6 H); MS (FAB, G/TG-DMSO, m/z, relative intensity): 412 ([M+1] + , 100); HRMS: Calcd. for C24H3QN05- M+ 412.2123 : found 412.2139. Step F: A solution of [CpRu(η - 4-chlorophenylpropionic acid)]PF6 1c (2.0 g, 4.03 mmol) in dry DMF (20 mL) was treated with HOBt (835 mg 6.0 mmol, 1.5 equiv.) and Hunigs base (2.06 g, 2.95 mL, 16 mmol, 4.0 equiv.) The reaction mixture was cooled to 0° C and treated with EDCI (1.15 g, 6.0 mmol, 1.5 equiv.) The reaction mixture was stirred at 0°C for 30 min. and the amine hydrochloride was added 33f (1.8 g, 4.03 mmol, 1.0 equiv.) in dry DMF (10 mL). The reaction mixture was stirred at rt. for 12 h and the DMF was distilled out vacuo. The residue was diluted with aq. HCl (1 M, 100 mL) and extracted into CH2CI2 (3x100 mL). The combined organic layers were extracted with aq. NaHCO3 (3x50 mL) , brine (100 mL), dried (Na2SO4), filtered, concentrated in vacuo to yield a brown solid 33g (3.5 g) which was used for cyclization; MS: Electron spray, m/z, relative intensity) 743 [(M-PF6) + , 100], 304 (60); HRMS: Calcd. for C38H4l NO6CI 102 Ru (M-PFβ) + 744.1666 : found 744.1694. Step G: 33g 33h A solution of η .6 -ruthenium complex 33g (3.5 g, 3.93 mmol) in dry DMF (300 mL) was degassed with dry N2 and treated with CS2CO3 (6.5 g, 19.95 mmol, 5.0 equiv.) and stirred at rt. for 16 h. The reaction mixture was concentrated in vacuo to remove the DMF and the residue was diluted with H2O (100 mL). The reaction mixture was extracted with CH2CI2 (3x100 mL). The combined CH2CI2 layers were extracted with brine, dried (Na2SO4), filtered, concentrated in vacuo to yield 33h which was directly used for photolysis; MS: (Electron spray, m/z, relative intensity) 708 [(M-PF6) + , 100]; HRMS: Calcd. for C38H40NO6I O2RU (M-PF6)+ :708.1892 ; found: 708.1918. Step H: 33h 33i A solution of cyclized ruthenium complex 33h (3.5g, 3.9 mmol) in CH3CN (60 mL) was degassed and photolyzed in a quartz tube at λ=350 nm in two batches for 48 h each. The reaction mixture were pooled together and purified by chromatography (Siθ2, CH2Cl2/Et2θ 9/1) to yield cyclic depsipeptide as a mixture of diastereomers. (700 mg, 34%). The diastereomers were separated by additional chromatography (Hexanes/CH2Cl2/ Et2θ 6:3:1) to yield the two diastereomers 33i (370 mg, 18%) and 33j (216 mg 11%) as colorless solid. Rf 0.28 (Hexanes: EtOAc: 3:2) ; [CC]D= 25 (c 0.15, CHCI3, 20 °C): IR (neat, cm "1 ) 3329 (w), 2960 (m) 2926 (s), 2854 (s), 1745 (s), 1680 (m), 1589 (m), 1506 (m), 1446 (m), 1365 (w), 1259 (s) 1099 (m), 1030 (s), 800 (s), 752 (m), 698 (w) 619 (w): 1 H NMR (CDCI3, 300 MHz, δ) 7.36-7.23 (m, 5 H), 7.18- 6.99 (m, 4 H), 6.81 (d, 1 H, J=7.5 Hz), 6.74 (dd, 1 H, J=2.7, 5.7 Hz), 6.30 (s, 1 H), 5.75 (d, 1 H, J=7.2 Hz), 5.61 (dd, 1 H, J=2.4 Hz, 5.4 Hz), 5.18, 5.14 (AB, 2 H, J=12.3 Hz), 4.23 (dd, 1 H, J=4.2 Hz, 3.3 Hz), 3.26-3.01 (m, 2 H), 2.98-2.85 (m, 2 H), 2.68-2.64 (m, 1 H) 2.38-2.34 (m, 1 H),1.96-1.5 (m, 6 H), 1.51-0.96 (M, 5H) 13 C NMR: (CDCI3, 75 MHz, δ, ppm) 177.3, 171.1 , 168.7, 159.8, 155.3, 138.6, 135.4, 134.9, 131.2, 129.7, 129.2, 128.7, 126.6, 126.1 , 123.3, 120.8, 120.8, 117.5, 114.2, 71.8, 57.5, 56.9, 41.5, 39.0, 35.7, 32.6, 31.3, 29.0, 27.6, 26.0, 25.9. FAB (NBA/DMSO, m/e, relative intensity) 542 [(M+1)+ 100], 514 (15), 450 (5), 307 (8), 232 (5), 154.1 (17), 136 (14) HRMS: Calcd for C33H36NO6 (M+1) + 542.2543: found: 542.2541 CHN Calcd for C33H35NO6.O.5H2O; C 71.98% H 6.59% N 2.54%; :Found C 72.56% H 7.05% N 2.63%. Step I: 33i 33k A solution of benzyl ester 33i (360 mg, 0.66 mmol) in CH3OH/EtOAc (1 :1 , 50 mL), was treated with Pd(OH)2 and hydrogenated (50 psi) for 12 h. The reaction mixture was filtered through a plug of celite and the cake was rinsed with CH3OH/CH2CI2 (1 :1 , 50 mL). The filtrate was concentrated in vacuo and the residue 33k (330 mg) was used for coupling without purification. Rf : 0.58 (CH3OH/CH2CI2 1 :19): MS: (Electron spray, m/z, relative intensity) 827.2 [(M+1) + , 100], 694 (20), 539 (40), 466 (10), 174 (70). HRMS: Calcd for C46H58N4O10 (M+1) + 827.4231 : found: 827.4215. Step J: A solution of acid 33k (165 mg, 0.31 mmol) in dry DMF (5.0 mL) and CH2CI2 (5.0 mL) was treated with HOBt (83 mg, 0.46 mmol, 1.5 equiv.) and cooled to 0 °C and Hϋnigs base (159 mg, 1.23 mmol, 4.0 equiv., 229 μL) was added. To this mixture was added EDCI (89 mg, 0.47 mmol, 1.5 equiv.) and the reaction mixture was stirred at 0° C for 1 h and treated with the amine hydrochloride B (159 mg, 0.372 mmol, 1.2 equiv.). The reaction mixture was stirred at rt. for 48 h and concentrated in vacuo to remove DMF and CH2CI2. The residue was diluted with water and extracted with CH2CI2 (3x50 mL). The combined organic layer were extracted with aq. HCl (1 M, 3x50 mL), aq. NaOH (1 M, 3x50 mL) brine (100 mL) and concentrated in vacuo. The residue 33I was oxidized without further purification. Step K: A solution of alcohol 331 (330 mg, 0.4 mmol) in CH2CI2 (5.0 mL) was treated with Dess-Martin reagent (424 mg, 1.00 mmol, 2.5 equiv.). The reaction mixture was stirred at rt. for 1 h and diluted with aq. NaHCθ3 (50 mL) and aq. Na2S2θ3 (50 mL). The reaction mixture was stirred at rt for 20 min and the reaction mixture was extracted with CH2CI2 (3x50 mL). The combined organic layers were extracted with brine (50 mL), dried (Na2S04), filtered, concentrated in vacuo and the residue was purified by chromatography (Siθ2, EtOAc/Hexanes 1 :1) to yield ketoamide 33 (180 mg, 55%) of a colorless solid. Rf : 0.63 (CH3OH/CH2CI2 1 :19); MS (Electron spray, m/z relative intensity): 857.2 ([M+CH30H] "1" , 40), 825.2 ([M+1] + 100). Example 34: Preparation of Compound of Formula 34: Step A: A solution of oxidized depsipeptide 33 (160 mg, 0.2 mmol) in dry CH2CI2 (5.0 mL) was treated with TFA (5.0 mL) and stirred at rt. for 7 h. The reaction mixture was concentrated in vacuo and the residue was repeatedly dissolved in CH3θH/CH2Cl2/Hexanes (1 :1 :1) and concentrated in vacuo several times to yield a tan colored solid 34 (133 mg, 86%) which was dried in vacuo MS: (Electron spray, m/z relative intensity): 769.2 [(M+1) + ,100], 481 (5), 269 (25) 191 (90). Example 35: Preparation of Compound of Formula 35: Step A: A solution of acid 33k (165 mg, 0.31 mmol) in dry DMF (5.0 mL) and CH2CI2 (5.0 mL) was treated with HOBt (83 mg, 0.46 mmol, 1.5 equiv) and cooled to 0 °C and Hϋnigs base (159 mg, 1.23 mmol, 4.0 equiv., 229 μL) was added. To this mixture was added EDCI (89 mg, 0.47 mmol, 1.5 equiv.) and the reaction mixture was stirred at 0° C for 1 h and treated with the amine hydrochloride A (159 mg, 0.372 mmol, 1.2 equiv.). The reaction mixture was stirred at rt. for 48 h and concentrated in vacuo to remove DMF and CH2CI2. The residue was diluted with water and extracted with CH2CI2 (3x50 mL) The combined organic layer was extracted with aq. HCl (1 M, 3x50 mL), aq. NaOH (1 M, 3x50 mL), brine (100 mL) and concentrated in vacuo. The residue 35a was oxidized without further purification. MS: (Electron spray, m/z relative intensity): 798.2 [(M+1) + , 30], 479 (10), 391 (20) 180 (100). Step B: A solution of alcohol 35a (190 mg, 0.24 mmol) in CH2CI2 (10 mL) was treated with Dess-Martin reagent (423 mg 1.0 mmol, 4.0 equiv.) and stirred at rt. for 1 h. The reaction mixture was diluted with aq. NaHC03 (50 mL) and extracted in CH2CI2 (3x50 mL). The combined organic layers were extracted with aq. satd. Na2S2θ3, brine (3x50 mL), dried (Na2Sθ4), filtered concentrated in vacuo and purified by chromatography (Siθ2, acetone/Hexanes 3:7->1 :1 to yield oxidized product 35 (163 mg, 86%) as a colorless solid. MS (Electron spray, m/z relative intensity): 796 [(M+1) + , 100], 508 (20), 269 (20). Example 36: Preparation of Compound of Formula 36: 36 Step A: 36a 36b A solution of BH3.THF (1 M in THF, 100 mmol, 100 mL, 3.0 equiv.) was added dropwise to a solution of alkene (5.0 g, 35 mmol) in THF (100 mL) at 0° C and stirred for 1 h. The reaction mixture was treated with ethanol (20 mL) dropwise. After the evolution of hydrogen gas was complete the reaction mixture was treated with pH 7 buffer (100 mL) and H2O2 (30 volumes, 100 mL). The reaction mixture was stirred at rt. for 4 h and quenched with aq. HCl (100 mL). The aqueous layer was extracted with Et2θ (3x100 mL). The combined ether layers were extracted with aq. NaOH (1 M, 100 m L), brine (100 mL), dried (MgS04), filtered, concentrated in vacuo and purified by chromatography (Siθ2, EtOAc/Hexanes 2/3) to yield alcohol as a colorless liquid (2.9 g, 52%). A solution of the hydroxylated ester in THF/H2O/CH3OH (100 mL, 1 :1 :1) was treated with UOH.H2O (2.1g, 51.2 mmol, 3.0 equiv.) and stirred at rt. for 3 d. The reaction mixture was concentrated in vacuo and the aqueous layer was extracted with ether (2x40 mL). The aqueous layer was acidified to pH ~1 and extracted into EtOAc (3x50 mL). The combined organic layers were extracted with brine (100 mL), dried (MgS04) filtered concentrated in vacuo and the residue 36b was used as is for coupling in Step B. 1 H NMR (300 MHz, CD 3 OD, δ) 3.53 (t, 2 H, J=6.6 Hz), 2.72 (t, 2 H, J=7.2 Hz), 1.59 (t, 2 H, J=7.5 Hz), 1.5 (t, 2 H, J=7.5 Hz), 1.38-1.33 (m, 6 H). Step B: A solution of ω-hydroxyl heptanoic acid 36b (1.01g, 6.93 mmol) in CH2CI2 (40 mL) was treated with Hϋnigs base (1.97 g, 15.24 mmol, 2.2 equiv. 2.81 mL) and amine hydrochloride 33f (3.1g, 6.93 mmol, 1.0 equiv.). The reaction mixture was cooled to 0 °C and treated with PyBrOP (3.22g, 6.93 mmol, 1.0 equiv.) The reaction mixture was stirred overnight at rt. and the reaction mixture was concentrated in vacuo. The residue was purified by chromatography (EtOAc/Hexanes 1 :1) to yield depsipeptide 36c (2.5 g, 66%) as a colorless viscous oil. 1 H NMR (CD3OD, 400 MHz, δ, ppm) 8.07 (t, 1 H), 7.33-7.21 (m, 4 H), 7.09-7.02 (m, 1 H), 6.67-6.63 (m, 3 H), 5.25-5.06 (m, 1 H), 5.08 (q, 2 H, J=7.5 Hz), 4.36-4.33 (m, 1 H), 3.51 (dd, 2 H, J=5.4 Hz, 0.9 Hz), 3.11-2.96 (m, 2 H), 2.22-2.17 (m, 1 H), 1.99-0.90 (m, 14 H). 13 C NMR : (CD3OD, 75 MHz, δ, ppm, mixture of diastereomers): 172.1 , 172.0, 171.8, 171.1 , 170.9, 169.5, 169.3, 157.1 , 157.0, 137.3, 137.0, 135.3, 135.2, 129.2, 129.1 , 128.2, 128.0, 127.9, 120.3, 120.0, 116.0, 115.9, 113.6, 94.8, 73.6, 73.4, 66.8, 66.7, 60.2, 57.3, 39.6, 36.7, 28.9, 28.0, 25.6, 20.9, 19.5, 13;. MS (FAB, NBA DMSO, m/z rel. int): 562. [(M+Na)+, 20], 540. [(M+1)+, 100), 412 (15), 240 (50), 112 (80). Step C: A solution of alcohol 36c (2.5g, 4.63 mmol) in dry CH2CI2 (50 mL) was treated with triphenylphosphine (2.67 g, 10.2 mmol, 2.2 equiv.) under N2 and cooled to 0°C. The reaction mixture was treated with DEAD (1.61g, 9.26 mmol, 2.0 equiv.) in CH2CI2 (30 mL). The reaction mixture was warmed to rt. and stirred for 2h. It was concentrated in vacuo and purified by chromatography (Et2θ/Hex 1 :3) to yield cyclic product 36d (530 mg, 21%) as a colorless solid. MS (FAB, NBA, DMSO, m/z rel. int), 522 [(M+1)+, 100], 494. (60), 268 (20), 222(20); HRMS calcd. for C31H40NO6 :( M+1)+: 522.2856; Found: 522. 2864. Step D: 36d 36e A solution of the benzyl ester (242 mg, 0.47 mmol) in methanoi (30 mL) was treated with Pd/C (10 wt %) and hydrogenated on a Parr at 40 psi for 14h. The reaction mixture was filtered through a plug of celite and the filtrate was concentrated in vacuo to yield a colorless solid 36e (181 mg, 93%) which was used for coupling. Step E: 36f A solution of the hydrolyzed acid 36e (167 mg, 0.39 mmol) in CH2CI2 (4.0 mL) was treated with HOOBt (95 mg, 0.58 mmol, 1.5 equiv.) and cooled to 0 °C and Hϋnigs base (202 mg, 1.56 mmol, 4.0 equiv. 288 μL) was added. To this mixture was added EDCI (111 mg, 0.58 mmol, 1.5 equiv.) and the reaction mixture was stirred at 0° C for 0.5 h and treated with the amine hydrochloride (186 mg, 0.47 mmol, 1.20 equiv.). The reaction mixture was stored in freezer for 24 h and concentrated in vacuo to remove DMF and CH2CI2. The residue was diluted with aq. HCl (2M, 30 mL) and extracted with CH2CI2 (3x50 mL) The combined organic layer was extracted with aq. HCl (2M, 30 mL), aq. NaOH (1 M) brine (2x50 mL), dried (MgS04) and concentrated in vacuo. The residue 36f (100 mg) was oxidized without further purification. HRMS calcd. for C42H60 5O9 (M+1)+: 778.4391 ; Found: 778.4399. Step F: 36 A solution of alcohol 36f (100 mg, 0.13 mmol) in CH2CI2 (5.0 mL) was treated with Dess-Martin reagent (100 mg, 0.0.23 mmol, 1.8 equiv.). The reaction mixture was stirred at rt. for 2 h and diluted with aq. NaHCθ3 (15 mL) and aq. Na2S2θ3 (15 mL). The reaction mixture was concentrated in vacuo and the residue was purified by chromatography (Siθ2, acetone/Hexanes: 3:7) to yield ketoamide 36 (61 mg, 61%) as a colorless solid; MS (FAB, NBA/DMSO, m/z rel. int): 776 [(M+1 )+, 100], 731 (10), 598 (25), 570 (15), 485 (20), 358 (20), 247 (50). Example 37: Preparation of Compound of Formula 37: 37 Step A: 33j 37a A solution of benzyl ester 33j (230 mg, 0.42 mmol) in CH3θH/EtOAc (1:1, 50 mL), was treated with Pd(OH)2 and hydrogenated (50 psi). for 12 h. The reaction mixture was filtered through a plug of celite and the cake was rinsed with CH3OH/CH2CI2 (1 :1 , 50 mL). The reaction mixture was concentrated in vacuo and the residue 37a (177 mg, 93%) was used for coupling without purification. Step B: A solution of acid 37a (177 mg, 0.33 mmol) in dry DMF (5.0 mL) and CH2CI2 (5.0 mL) was treated with HOBt (88 mg, 0.49 mmol, 1.5 equiv) and cooled to 0 °C and Hϋnigs base (175 mg, 1.35 mmol, 4.0 equiv, 251 μL) was added. To this mixture was added EDCI (95 mg, 0.49 mmol, 1.5 equiv) and the reaction mixture was stirred at 0° C for 1 h and treated with the amine hydrochloride B (170 mg, 0.39 mmol, 1.2 equiv.). The reaction mixture was stirred at rt for 48 h and concentrated in vacuo to remove DMF and CH2CI2. The residue was diluted with water and extracted with CH2CI2 (3x50 mL) The combined organic layer was extracted with aq. NaOH (1M, 2x50 mL), brine (100 mL) and concentrated in vacuo. The residue 37b (315 mg) was oxidized without further purification. Step C: A solution of alcohol 37b (315 mg, 0.4 mmol) in CH2CI2 (5.0 mL) was treated with Dess-Martin reagent (424 mg, 1.00 mmol, 2.5 equiv.). The reaction mixture was stirred at rt. for 1 h and diluted with aq. NaHC03 (50 mL) and aq. Na2S2θ3 (50 mL). The reaction mixture was stirred at rt. for 20 min and the reaction mixture was extracted with CH2CI2 (3x50 mL). The combined organic layers were extracted with brine, dried (Na2Sθ4), filtered concentrated in vacuo and the residue was purified by chromatography (Siθ2, EtOAc/Hexanes 1 :1) to yield ketoamide 37 (210 mg, 66%) of a colorless solid. Rf : 0.63 (CH3OH/CH2CI2 1 :19); MS: (Electron spray, m/z relative intensity): 857 ([M+CH3θH] + , 33), 825 ( [M+1] + 40), 191 (100). Example 38: Preparation of Compound of Formula 38: Step A: 38 A solution of oxidized depsipeptide 37 (200 mg, 0.24 mmol) in dry CH2CI2 (5.0 mL) was treated with TFA (5.0 mL) and stirred at rt. for 7 h. The reaction mixture was concentrated in vacuo and the residue was repeatedly dissolved in CH3θH/CH2Cl2/Hexanes (1 :1 :1) and concentrated in vacuo several times to yield a tan colored solid 38 (130 mg, 87%) which was dried in vacuo; MS: (Electron spray, m/z relative intensity): 769 ([M+1] + , 45), 294 (45), 191 (100). Example 39: Preparation of Compound of Formula 39: Step A: A solution of acid 22 (40 mg, 0.06 mmol) in CH2CI2 (0.5 mL) and DMF (0.5 mL) was cooled to 0° C and treated with Me2NH.HCI (15 mg, 0.18 mmol, 3.0 equiv) and Hϋnigs base (31 mg, 0.24 mmol, 44 μL, 4.0 equiv). The reaction mixture was then treated with PyBrOP (55 mg, 0.12 mmol, 2.0 equiv) and stored for 12 h in the freezer. The yellow reaction mixture was concentrated in vacuo and the residue was purified by chromatography (Siθ2, EtOAc/Hexanes gradient 3:2 —>1 :0) to obtain impure product which was purified once again using (acetone/Hexanes 1 :6) to yield dimethyl amide 39 as a colorless solid (14 mg, 35%). MS: (Electron spray, m/z relative intensity): 791 [(M+1) + , 50], 391 (40), 276 (50), 176 (100). Example 40: Preparation of Compound of Formula 40: 40 General procedure for solid-phase coupling reactions: The synthesis was done in a reaction vessel which was constructed from a polypropylene syringe cartridge fitted with a polypropylene frit at the bottom. The Fmoc-protected amino acids were coupled under standard solid-phase techniques. Each reaction vessel was loaded with 100 mg of the starting Fmoc- Sieber resin (approximately 0.035 mmol). The resin was washed with 2 mL portions of DMF (2 times). The Fmoc protecting group was removed by treatment with 2 mL of a 20 % v/v solution of piperidine in DMF for 20 min. The resin was washed with 2 mL portions of DMF (4 times). The coupling was done in DMF (2 mL), using 0.12 mmol of Fmoc-amino acid, 0.12 mmol of HATU and 0.24 mmol of DIPEA. After shaking for 2 h, the reaction vessel was drained and the resin was washed with 2 mL portions of DMF (4 times). The coupling cycle was repeated with the next Fmoc-amino acid or capping group. Scheme 10: FmocHN-P 40a 40b 40c 40d 40e Fmoc-Sieber resin 40a (0.035 mmol) was treated with 2 mL of a 20 % v/v solution of piperidine in DMF for 20 min. followed by washing with 2 mL portions of DMF (4 times). DMF (2 mL) was added to the resin followed by Fmoc- phenylglycine (0.12 mmol), HATU (0.12 mmol) and DIPEA (0.24 mmol). After shaking at room temperature for 2 h, the resin was washed with 2 mL portions of DMF (4 times) to afford resin-bound compound 40b. Resin-bound compound 40b was treated with 2 mL of a 20 % v/v solution of piperidine in DMF for 20 min. followed by washing with 2 mL portions of DMF (4 times). DMF (2 mL) was added to the resin followed by Fmoc-glycine (0.12 mmol), HATU (0.12 mmol) and DIPEA (0.24 mmol). After shaking at room temperature for 2 h, the resin was washed with 2 mL portions of DMF (4 times) to afford resin-bound compound 40c. Resin-bound compound 40c was treated with 2 mL of a 20 % v/v solution of piperidine in DMF for 20 min. followed by washing with 2 mL portions of DMF (4 times). DMF (2 mL) was added to the resin followed by N-Fmoc-propylisoserine (0.12 mmol), HATU (0.12 mmol) and DIPEA (0.24 mmol). After shaking at room temperature for 2 h, the resin was washed with 2 mL portions of DMF (4 times) to afford resin-bound compound 40d. Resin-bound compound 40d was treated with 2 mL of a 20 % v/v solution of piperidine in DMF for 20 min. followed by washing with 2 mL portions of DMF (4 times). DMF (2 mL) was added to the resin followed by Fmoc-lysine(Dde) (0.12 mmol), HATU (0.12 mmol) and DIPEA (0.24 mmol). After shaking at room temperature for 2 h, the resin was washed with 2 mL portions of DMF (4 times) to afford resin-bound compound 40e. Resin-bound compound 40e was treated with 2 mL of a 20 % v/v solution of piperidine in DMF for 20 min. followed by washing with 2 mL portions of DMF (4 times). DMF (2 mL) was added to the resin followed by Fmoc-cyclohexylglycine (0.12 mmol), HATU (0.12 mmol) and DIPEA (0.24 mmol). After shaking at room temperature for 2 h, the resin was washed with 2 mL portions of DMF (4 times) to afford resin-bound compound 40f. Resin-bound compound 40f was treated with 2 mL of a 20 % v/v solution of piperidine in DMF for 20 min. The resin was washed with 2 mL portions of DMF (4 times) to provide resin-bound compound 40g. Resin-bound compound 40g was treated with 2 mL portions of a 2 % v/v solution of hydrazine in DMF for 5 min. (3 times). The resin was washed with 2 mL portions of DMF (4 times) to provide resin-bound compound 40h. Resin-bound compound 40h was treated with 0.035 mmol of glutaric acid, 0.07 mmol of HATU and 0.14 mmol of DIPEA in 2 mL of DMF at room temperature for 16 h. The resin was washed with 2 mL portions of DMF (4 times), THF (4 times) and DCM (4 times) to provide resin-bound compound 40i. Resin-bound compound 40i was treated with a solution of 0.14 mmol of Dess-Martin periodinane and 0.14 mmol of t-BuOH in 2 mL of DCM at room temperature for 4 h. The resin was washed with 2 mL portions of a 20 % v/v solution of iPrOH in DCM, THF, a 50 % v/v solution of THF in water (4 times), THF (4 times) and DCM (4 times) to provide resin-bound compound 40j. The resin-bound compound 40j was treated with 4 mL of a 2 % v/v solution of TFA in DCM for 5 min. The filtrate was added to 1 mL of AcOH and the solution was concentrated by vacuum centrifugation to provide compound 40 (0.0117 g, 48 % yield). MS (LCMS-Electrospray) 698.2 MH + . Example 41 - 53: Preparation of Compounds of Formula 41 to 53: 41 43 44 50 51 Compounds 41-53 were synthesized using solid phase methodology similar to the procedure outlined for the synthesis of Example 40. Example 54: Preparation of Compound of Formula 54: Step A: To a stirred solution of Boc-Cyclohexylglycine-OH (2.33 g, 9.07 mmol) in DMF (20 mL) and CH CI2 (20 mL) was added HOBT (1.48 g, 9.07 mmol), EDCI (1.91 g, 9.97 mmol) and NMM (2.99 mL, 27.2 mmol). The solution was stirred at - 20°C for 10 minutes, followed by addition of H-Lys(Z)-OMe.HCI and stirred for half an hour at -20°C and kept in freezer overnight. The solution was then concentrated to dryness, followed by extraction with EtOAc, saturated NaHC03. The combined organic layer was washed with H2O, brine, dried over Na2Sθ4 and concentrated to dryness to give a white solid (4.83 g, MH + =534.1). Step B: To a stirred solution of 54b (4.86 g, 8.76 mmol) in MeOH (10 mL) and H2O (7 mL) was added LiOH (70 mg, 11.4 mmol). The white precipitate was formed and the solution was allowed to stir at room temperature overnight and then concentrated to dryness. This crude material was then partitioned between CH 2 CI 2 and water. The organic layer was separated and washed with brine, dried over Na2Sθ4, filtered and concentrated to dryness to give 54c (4.55 g, MH + =520.1). Step C: To a stirred cooling solution of 54c (4.3 g, 8.27 mmol) in DMF (40 mL) and CH2CI2 (40 mL) at -20 °C was added HOBT (1.35 g, 8.27 mmol), EDCI (1.74 g, 9.1 mmol) and NMM ( 2.73 mL, 8.27 mmol). The resulting solution was stirred at - 20 °C for 10 minutes, followed by addition of amine G (2.32 g, 8.27 mmol) and 1 b8 stirred at -20 °C for half an hour and kept in freezer overnight. The work up procedure from step A was followed to give a 54d (6.21 g, MH + =746.2). 54e The solution of 54d (6.16 g, 8.26 mmol) in 4N HCI/Dioxane (40 mL) was stirred at room temperature for 1 hr and concentrated to dryness to give a crude product 54e (5.70 g, 100% yield, MH + =646.3). Step E: To a stirred cooling solution of Boc-Glu(OBn)-OH in DMF (25 mL) and CH2CI2 (25 mL) at -20 °C, was added HOBT (1.29 g, 7.92 mmol), EDCI (1.66 g, 8.71 mmol) and NMM(2.61 mL, 23.7 mmol). The resulting solution was stirred for 10 minutes at -20°C, followed by addition 54e (5.4 g, 7.916 mmol) and stirred for half an hour at -20°C and kept in freezer overnight. Followed the work up procedure from step A to give a crude product (7.14 g, 93.5% yield). Step F: To a stirred solution of 54f ( 6.9 g, 7.15 mmol) in absolute EtOH (350 mL), was added 10% Pd/C (2.8 g) in 50% H2O (w/w). The resulting solution was purged with H2 and stirred under H2 balloon overnight. The solution was then filtered through celite and the filtrate was washed with EtOH/CH2Cl2 and then concentrated to dryness to give a white solid (1.44 g). The solid was washed with 25% H 2 0/ MeOH and filtered through sintered funnel, then freezed and lyophilized to give 54g (4.12 g, 77.5% yield, MH + =743.2). Step G: To a stirred cooling solution of 54g (0.5 g, 6.7 mmol) in DMF (50 mL) and CH2CI2 (50 mL) at -20 °C, was added HOBT (0.219 g, 1.34 mmol), EDCI (0.271 g, 1.41 mmol) and NMM (0.296 mL, 2.69 mmol). The resulting solution was stirred at -20 °C for 25 minutes and then kept in freezer overnight. The solution was concentrated to dryness, followed by extraction with EtOAc saturated NaHC03. The combined organic layer was then concentrated to dryness to give 54h (254 mg, MH + =725.2). Step H: To a stirred solution of 54h (0.2 g, 0.27 mmol) in anhydrous CH2CI2 (20 mL), was added Dess-Martin periodinane (0.234 g, 0.55 mmol). The resulting solution was stirred at room temperature for 1 hr. To this solution was added dropwise over half an hour the solution of H2O (0.010 mL) in CH2CI2 (20 mL) and stirred vigorously for additional 2 hrs. The solution was then stirred for half an hour with 50% Na2S2θ3/50% sat. NaHC03. The organic layer was separated and washed with H2O, brine, dried over Na2Sθ4, filtered, concentrated to dryness and purified by column chromatography on silica gel, eluting with 10% MeOH/CH2Cl2 to yield 54 (17 mg, 62% MH + =723.2). Assay for HCV Protease Inhibitory Activity: Spectrophotometric Assay: Spectrophotometric assays for the HCV serine protease was performed on the inventive compounds by following the procedure described by R. Zhang et al, Analytical Biochemistry, 270 (1999) 268-275, the disclosure of which is incorporated herein by reference. The assay based on the proteolysis of chromogenic ester substrates is suitable for the continuous monitoring of HCV NS3 protease activity. The substrates were derived from the P side of the NS5A-NS5B junction sequence (Ac-DTEDVVX(Nva), where X = A or P) whose C-terminal carboxyl groups were esterified with one of four different chromophoric alcohols (3- or 4-nitrophenol, 7-hydroxy-4-methyl-coumarin, or 4- phenylazophenol). Presented below are the synthesis, characterization and application of these novel spectrophotometric ester substrates to high throughput screening and detailed kinetic evaluation of HCV NS3 protease inhibitors. Materials and Methods: Materials: Chemical reagents for assay related buffers were obtained from Sigma Chemical Company (St. Louis, Missouri). Reagents for peptide synthesis were from Aldrich Chemicals, Novabiochem (San Diego, California), Applied Biosystems (Foster City, California) and Perseptive Biosystems (Framingham, Massachusetts). Peptides were synthesized manually or on an automated ABI model 431 A synthesizer (from Applied Biosystems). UV/VIS Spectrometer model LAMBDA 12 was from Perkin Elmer (Norwalk, Connecticut) and 96-well UV plates were obtained from Corning (Corning, New York). The prewarming block was from USA Scientific (Ocala, Florida) and the 96-well plate vortexer was from Labline Instruments (Melrose Park, Illinois). A Spectramax Plus microtiter plate reader with monochrometer was obtained from Molecular Devices (Sunnyvale, California). Enzyme Preparation: Recombinant heterodimeric HCV NS3/NS4A protease (strain 1a) was prepared by using the procedures published previously (D. L. Sali et al, Biochemistry, 37 (1998) 3392-3401). Protein concentrations were determined by the Biorad dye method using recombinant HCV protease standards previously quantified by amino acid analysis. Prior to assay initiation, the enzyme storage buffer (50 mM sodium phosphate pH 8.0, 300 mM NaCl, 10% glycerol, 0.05% lauryl maltoside and 10 mM DTT) was exchanged for the assay buffer (25 mM MOPS pH 6.5, 300 mM NaCl, 10% glycerol, 0.05% lauryl maltoside, 5 μM EDTA and 5 μM DTT) utilizing a Biorad Bio-Spin P-6 prepacked column. Substrate Synthesis and Purification: The synthesis of the substrates was done as reported by R. Zhang et al, (ibid.) and was initiated by anchoring Fmoc-Nva- OH to 2-chlorotrityl chloride resin using a standard protocol (K. Barlos et al, Int. J. Pept. Protein Res., 37 (1991), 513-520). The peptides were subsequently assembled, using Fmoc chemistry, either manually or on an automatic ABI model 431 peptide synthesizer. The N-acetylated and fully protected peptide fragments were cleaved from the resin either by 10% acetic acid (HOAc) and 10% trifluoroethanol (TFE) in dichloromethane (DCM) for 30 min, or by 2% trifluoroacetic acid (TFA) in DCM for 10 min. The combined filtrate and DCM wash was evaporated azeotropically (or repeatedly extracted by aqueous Na 2 C0 3 solution) to remove the acid used in cleavage. The DCM phase was dried over Na 2 S0 4 and evaporated. The ester substrates were assembled using standard acid-alcohol coupling procedures (K. Holmber et al, Ada Chem. Scand., B33 (1979) 410-412). Peptide fragments were dissolved in anhydrous pyridine (30-60 mg/ml) to which 10 molar equivalents of chromophore and a catalytic amount (0.1 eq.) of para- toluenesulfonic acid (pTSA) were added. Dicyclohexylcarbodiimide (DCC, 3 eq.) was added to initiate the coupling reactions. Product formation was monitored by HPLC and found to be complete following 12-72 hour reaction at room temperature. Pyridine solvent was evaporated under vacuum and further removed by azeotropic evaporation with toluene. The peptide ester was deprotected with 95% TFA in DCM for two hours and extracted three times with anhydrous ethyl ether to remove excess chromophore. The deprotected substrate was purified by reversed phase HPLC on a C3 or C8 column with a 30% to 60% acetonitrile gradient (using six column volumes). The overall yield following HPLC purification was approximately 20-30%. The molecular mass was confirmed by electrospray ionization mass spectroscopy. The substrates were stored in dry powder form under desiccation. Spectra of Substrates and Products: Spectra of substrates and the corresponding chromophore products were obtained in the pH 6.5 assay buffer. Extinction coefficients were determined at the optimal off-peak wavelength in 1 -cm cuvettes (340 nm for 3-Np and HMC, 370 nm for PAP and 400 nm for 4-Np) using multiple dilutions. The optimal off-peak wavelength was defined as that wavelength yielding the maximum fractional difference in absorbance between substrate and product (product OD - substrate ODVsubstrate OD). Protease Assay: HCV protease assays were performed at 30°C using a 200 μl reaction mix in a 96-well microtiter plate. Assay buffer conditions (25 mM MOPS pH 6.5, 300 mM NaCl, 10% glycerol, 0.05% lauryl maltoside, 5 μM EDTA and 5 μM DTT) were optimized for the NS3/NS4A heterodimer (D. L. Sali et al, ibid.)). Typically, 150 μl mixtures of buffer, substrate and inhibitor were placed in wells (final concentration of DMSO • 4 % v/v) and allowed to preincubate at 30 °C for approximately 3 minutes. Fifty μls of prewarmed protease (12 nM, 30°C) in assay buffer, was then used to initiate the reaction (final volume 200 μl).The plates were monitored over the length of the assay (60 minutes) for change in absorbance at the appropriate wavelength (340 nm for 3-Np and HMC, 370 nm for PAP, and 400 nm for 4-Np) using a Spectromax Plus microtiter plate reader equipped with a monochrometer (acceptable results can be obtained with plate readers that utilize cutoff filters). Proteolytic cleavage of the ester linkage between the Nva and the chromophore was monitored at the appropriate wavelength against a no enzyme blank as a control for non-enzymatic hydrolysis. The evaluation of substrate kinetic parameters was performed over a 30-fold substrate concentration range (-6-200 μM). Initial velocities were determined using linear regression and kinetic constants were obtained by fitting the data to the Michaelis-Menten equation using non-linear regression analysis (Mac Curve Fit 1.1 , K. Raner). Turnover numbers (k cat ) were calculated assuming the enzyme was fully active. Evaluation of Inhibitors and Inactivators: The inhibition constants (K.) for the competitive inhibitors Ac-D-(D-Gla)-L-l-(Cha)-C-OH (27), Ac-DTEDVVA(Nva)-OH and Ac-DTEDVVP(Nva)-OH were determined experimentally at fixed concentrations of enzyme and substrate by plotting V Q /VJ VS. inhibitor concentration ([I] o ) according to the rearranged Michaelis-Menten equation for competitive inhibition kinetics: VQ/V,- = 1 + [I] 0 /( j (1 + [S] 0 /K m )), where v 0 is the uninhibited initial velocity, Vj is the initial velocity in the presence of inhibitor at any given inhibitor concentration ([l] 0 ) and [S] 0 is the substrate concentration used. The resulting data were fitted using linear regression and the resulting slope, 1/(Kj(1+[S] c /K m ), was used to calculate the K, value. The obtained K| values for the various macrocycles of the present invention are given in the afore-mentioned Table 1 wherein the compounds have been arranged in the order of ranges of Kj values. From these test results, it would be apparent to the skilled artisan that the compounds of the invention have excellent utility as NS3-serine protease inhibitors. Cell Bioassay Method: The cell bioassays for the HCV serine protease was performed on the inventive compounds by following the procedure described by S. Agrawal et al, "Development and Characterization of Hepatitis C Virus Serine Protease Cell-based Trans-Cleavage Assay", Hepatology Supplement to Volume 30 (No. 4, Part 2, October 1999), Abstract No. 615 (Proceedings of AASLD 50 th Annual Meeting, Dallas, Texas, November 5-9, 1999), the disclosure of which is incorporated herein by reference. The assay was performed in HeLa/Huh7 cells that were co-transfected with a plasmid that expresses a reporter protein substrate containing the NS5A/5B cleavage recognition sequence and an 1 BNS4A 21 - 32 GS-GSNS 3 . 81 I17K expression vector and YFPnl as a internal standard protein to control cytotoxicity. Protease activity was measured by SDS- PAGE of total cell lysates followed by Western blot detection using a monoclonal antibody directed against the reporter substrate. Quantitation of substrate cleavage was performed by scanning the immunoblot on the phosphoimager. Materials: Plasmid DNAs pBFP-5A/5B-GFP: The reporter gene that expresses the substrate encodes a fusion protein comprised of an N' terminal blue fluorescent protein (BFP) domain and a C terminal green fluorescent protein (GFP) domain, separated by a 25 amino acids derived from the NS5A/5B cleavage recognition sequence. Both GFP and BFP are essentially homologous autofluorescent proteins that emit green or blue light, respectively, when excited by UV light of the appropriate wavelength. Four amino acid substitutions in the chromophore of GFP alter the emission wavelength and convert the protein to BFP. The substrate and the resulting GFP and BFP products can be detected in cell lysates by immunologic methods using a monoclonal antibody that recognizes both proteins. The BFP-5A/5B-GFP reporter gene contains the BFP and GFP autofluorescent protein coding sequences (Quantum Biotechnologies, Inc., Montreal, Canada) separated by the NS5A/5B cleavage recognition sequence, cloned between the Nhe I and Bam HI restriction endonuclease sites of the pQBI25 cloning vector (Quantum Biotechnologies, Inc.). Expression of the fusion protein is under the control of the CMV IE promoter-enhancer. The bovine growth hormone p (A) sequence of the vector provides the polyadenylation signal for the mRNA. The NS5A/5B cleavage sequence is: SSGADTEDVVCCSMSYTWTGALVTP. DNA sequencing was used to validate the clone. P1BOO2: 1bNS4A21-32GS-GS NS 3-81 117K: The subtype 1 b protease was cloned as an Xba1/Not1 fragment behind the CMV promoter in vector pC1 neo. YFPnl : YFPnl was purchased from CLONTECH (Palo Alto, California). Addition of third plasmid to the transfection supplies an internal standard protein to control for cytotoxicity and does not affect percentage of protease cleavage. Plasmid DNAs were maintained and propagated in DH5α cells (obtained from LifeTechnologies) in LB medium under the appropriate antibiotic selection, and purified using QIAfilter Plasmid Kits (Qiagen, Valencia, California). Cell Culture: HeLa cells were maintained and propagated in Eagle's Minimum Essential Media (EMEM; BioWhittaker, Walkersville, Maryland) supplemented with 10% fetal calf serum (FCS), 2 mM glutamine, and 100 u/ml penicillin-streptomycin (BioWhitaker), 2% NaHC0 3 . Huh7 cells were maintained and propagated in Dulbecco's Modified Eagle's medium (DMEM; BioWhittaker) supplemented with 10% fetal calf serum (FCS), 100u/ml penicillin-streptomycin (BioWhitaker) and 5 ml NEAA (100x; BioWhittaker)/L . SOP Procedure Day preceding transfection: HeLa cells were seeded in 24 well plates (Falcon 3047 plates) at a density of 6 x 10 4 cells/well and grown overnight at 37°C in a 5% C0 2 incubator. Day of transfection: Plasmid DNAs were diluted to a final concentration of 0.05 μg/μl in nuclease free water (Promega, Madison, Wisconsin, cat # P119C). 0.75 μg BFP-5A/5B-GFP was combined and mixed with 0.175 μg P1 B002 (0.23X) and 0.02μg of YFPnl . The DNAs were brought to a final volume of 60 μl with EMEM lacking FBS, glutamine, and antibiotics. A ratio of 5 μl volumes of SuperFect Reagent (Qiagen, cat # 301305) per total μgs of DNA was added and the mixture vortexed about 10 seconds and incubated 10 min. at room temperature to allow complex formation. While complex formation was taking place, growth medium from cell culture plates was aspirated and cells washed 1X with 1 ml PBS without Ca 2+ , Mg 2+ (BioWhitaker). 350 μl EMEM (supplemented with appropriate suplements- compleat medium) was added to the tube containing the transfection complexes and the mixture pipetted up and down 2-3 times. Total volume was transferred to one well of the 24 well culture plate. The HeLa cells were incubated with the transfection complexes for about 3 hr. at 37°C and 5% C02. The media containing the transfection complexes was removed from the cells by aspiration. The cells were washed once in about 1 ml PBS, the PBS was aspirated and 495 μl of complete EMEM was added followed by 5 μl compound/well. The cells were incubated 22-24 hr. at 37°C and 5% C02. Preparation of Cell Lysates The medium from each well was aspirated and washed once 1x with DPBS. Cells were harvested in 100 μl of 1x Tris-SDS-BME sample buffer (OWL separation system, Portsmouth, New Hampshire, cat # ER33) and transferred to microcentrifuge tubes. It was then boiled 3-5 min. to lyse cells. Loading was done at 10 μl/well on SDS-PAGE gel. The lysates were resolved by electrophoresis on 10 cm x 10 cm 12.5% SDS-PAGE (Owl Scientific, cat # OG-0125B) run at 30 mamp in Tris-Glycine-SDS buffer (Owl Scientific). Prior to use, PVDF membrane (Immobilon-P; .45μm pore size; Millipore, Bedford, Massachusetts) was soaked in 100% methanoi for 10 seconds and then the blot was placed in distilled water. The proteins were transferred to PVDF filter membranes (0.45μm, Millipore) at 108 mamp per gel for 90 minutes using a semi-dry electroblotter. Detection of Proteins bv ECF Western Blot (Amersham Pharmacia Biotech, Little Chalfont, England), catalog #RPN 5780). The PVDF filter membranes were blocked by 5% blocking reagent (from kit) in -10 ml PBS containing 0.05% Tween 20, pH 7.4 (Sigma Chemicals, St. Louis, Missouri, cat # 3563) for overnight at 2- 4 C in refrigerator. The next day, the membranes were rinsed briefly twice with TPBS containing 0.05% Tween 20 washing buffer, then washed three times each time 5 min. in PBS containing 0.05% Tween 20, pH 7.4. The membranes were incubated in 12 mis of a 1 :3000 dilution of anti-GFP monoclonal antibody for 30 minutes (Clontech, Palo Alto, California) in PBS containing 0.05% Tween 20, pH7.4 while at the same time 1% BSA (Albumin, bovine cat # A-2153 from Sigma) was added to reduce background. The membranes were washed briefly twice with TPBS, then thrice, for 5 min. each time, in TPBS washing buffer. The membranes were incubated in 12 mis of a 1 :600 dilution anti fluorescein-linked anti mouse Ig in TPBS for 30 minutes. The membranes were washed briefly with TPBS twice, then for 5 min. in TPBS washing buffer thrice. For signal amplification with ECF substrate membranes were incubated in 10 ml of 1 :2500 anti fluorescein alkaline phosphatase conjugate for 30 minutes. The membranes were rinsed briefly with TPBS twice, then 5 min. in TPBS washing buffer thrice. The ECF substrate solution was prepared as per manufacturer's instructions (aliquot and freeze), membranes were incubated for 2-3 minutes, excess reagent was drained off, then were blotted with filter papers, air-dried for 9-10 minutes and then scanned. Scanning the membrane: The blot was placed on the glass of phosphoimager Storm 860. The blue chemiluminiescent was set up, 200 pixcels size, 700 PMT voltage. The file was opened in ImageQuant and quantitated by creating squares around the bands representing the substrate (S), the product (P) and the internal control (IC). The % cleavage of the substrate was measured as P/(S+P)x100. The inhibition in cleavage due to drug was measured compared duplicate to drug controls included on each blot. A report was created in Excel. The results for some of the compounds are given below: Compound of Example 36: EC 50 = 9μm Compound of Example 35: EC 50 = 20μm From these test results, it would be apparent to the skilled artisan that the compounds of the invention have excellent utility as NS3-serine protease inhibitors.

Claims: Claims What is claimed is: 1. A macrocyclic compound, including enantiomers, stereoisomers, rotomers and tautomers of said compound, and pharmaceutically acceptable salts or solvates of said compound having the general structure shown in Formula I: wherein: E, X and Y may be independently present or absent, and if present are independently selected from the moieties: alkyl, aryl, alkyl-aryl, heteroalkyl, heteroaryl, aryl-heteroaryl, alkyl-heteroaryl, cycloalkyl, alkyl ether, alkyl-aryl ether, aryl ether, alkyl amino, aryl amino, alkyl-aryl amino, alkyl sulfide, alkyl-aryl sulfide, aryl sulfide, alkyl sulfone, alkyl-aryl sulfone, aryl sulfone, alkyl-alkyl sulfoxide, alkyl-aryl sulfoxide, alkyl amide, alkyl-aryl amide, aryl amide, alkyl sulfonamide, alkyl-aryl sulfonamide, aryl sulfonamide, alkyl urea, alkyl-aryl urea, aryl urea, alkyl carbamate, alkyl-aryl carbamate, aryl carbamate, alkyl -hydrazide, alkyl-aryl hydrazide, alkyl hydroxamide, alkyl-aryl hydroxamide, alkyl sulfonyl, aryl sulfonyl, heteroalkyl sulfonyl, heteroaryl sulfonyl, alkyl carbonyl, aryl carbonyl, heteroalkyl carbonyl, heteroaryl carbonyl, alkoxycarbonyl, aryloxycarbonyl, heteroaryloxycarbonyl, alkyiaminocarbonyl, arylaminocarbonyl, heteroarylaminocarbonyl or a combination thereof, with the proviso that E, X and Y may optionally be additionally substituted with moieties selected from the group consisting of aromatic, alkyl, alkyl-aryl, heteroalkyl, aryl-heteroaryl, alkyl- heteroaryl, cycloalkyl, alkyl ether, alkyl-aryl ether, alkyl sulfide, alkyl-aryl sulfide, alkyl sulfone, alkyl-aryl sulfone, alkyl amide, alkyl-aryl amide, alkyl sulfonamide, , alkyl amines, alkyl-aryl amines, alkyl-aryl sulfonamide, alkyl urea, alkyl-aryl urea, alkyl carbamate, alkyl-aryl carbamate, halogen, hydroxyl amino, alkyl carbazate, aryl carbazate; R 1 = COR 5 or B(OR) 2> wherein R 5 = H, OH, OR 8 , NR 9 R 10 , CF 3 , C F 5 , C F 7 , CF 2 R 6 , R 6 , COR 7 wherein R 7 = H, OH, OR 8 , CHR 9 R 10 , or NR 9 R 10 , wherein R 6 , R 8 , R 9 and R 10 are independently selected from the group consisting of H, alkyl, aryl, heteroalkyl, heteroaryl, cycloalkyl, cycloalkyl, arylalkyl, heteroarylalkyl, CH(R ')COOR , CHfR^CONFTR , CH(R ' ' )CONHCH(R 2' )COO R 11 , CH(R 1' )CONHCH(R 2' )CONR 12 R 13 , CH(R ')CONHCH(R 2' )R\ CH(R r )CONHCH(R 2' )CONHCH(R 3 )COO R 11 , CH(R ')CONHCH(R 2' )CONHCH(R 3' )CONR 12 R 13 , CH(R 1 ' )CONHCH(R 2' )CONHCH(R 3' )CONHCH(R 4' )COO R 11 , CH(R 1, )CONHCH(R 2, )CONHCH(R 3 )CONHCH(R 4' )CONR 12 R 13 , CH(R 1' )CONHCH(R 2' )CONHCH(R 3' )CONHCH(R 4' )CONHCH(R 5 )COO R 11 , CH(R 1' )CONHCH(R 2' )CONHCH(R 3' )CONHCH(R 4 )CONHCH(R 5 ) CONR 12 R 13 , wherein R 1' , R 2' , R 3' , R 4' , R 5' , R 11 , R 12 , R 13 , and R' are independently selected from a group consisting of H, alkyl, aryl, heteroalkyl, heteroaryl, cycloalkyl, alkyl-aryl, alkyl-heteroaryl, aryl-alkyl and heteroaralkyl; Z is selected from O, N, or CH; W may be present or absent, and if W is present, W is selected from C=0, C=S, S0 2 or C=NR; Q is (NR) P , O, S, CH 2 , CHR, CRR' or a double bond towards V; A is O, CH 2 , (CHR) p , (CHR-CHR') p (CRR') p , NR, S, S0 2 , C=0 or a bond; G is (CH 2 ) p , (CHR) p , (CRR') P , NR, O, S, S0 2 , S(0) 2 NH, C=0, or a double bond towards E or V; V is CH, CR or N; p is a number from 0 to 6; and R, R', R 2 , R 3 and R 4 are independently selected from the group consisting of H; C1-C10 alkyl; C2-C10 alkenyl; C3-C8 cycloalkyl; C3-C8 heterocycloalkyl, aryl, alkoxy, aryloxy, alkylthio, arylthio, amino, amido, ester, carboxylic acid, carbamate, urea, ketone, aldehyde, cyano, nitro; heteroaryl; alkyl-aryl; alkyl- heteroaryl; (cycloalkyl)alkyl and (heterocycloalkyl)alkyi, wherein said cycloalkyl is made of three to eight carbon atoms, and zero to six oxygen, nitrogen, sulfur, or phosphorus atoms, and said alkyl is of one to six carbon atoms; with said alkyl, heteroalkyl, alkenyl, heteroalkenyl, aryl, heteroaryl, cycloalkyl and heterocycloalkyl moieties may be optionally substituted, with said term "substituted" referring to optional and suitable substitution with one or more moieties selected from the group consisting of alkyl, alkenyl, alkynyl, aryl, aralkyl, cycloalkyl, heterocyclic, halogen, hydroxy, thio, alkoxy, aryloxy, alkylthio, arylthio, amino, amido, ester, carboxylic acid, carbamate, urea, ketone, aldehyde, cyano, nitro, sulfonamide, sulfoxide, sulfone, sulfonyl urea, hydrazide, hydroxamate and thiourea. 2. The compound of claim 1 , wherein R 1 = COR 5 , and R 5 is H, OH, COOR 8 , or CONR 9 R 10 . 3. The compound of claim 2, wherein R 1 = COCONR 9 R 10 , and is R 9 is H, R 10 is H, CH(R 1' )COOR 11 , CH(R 1' ) CONR 12 R 13 , CH(R 1' )CONHCH(R 2' )COOR 11 , CH(R 1 )CONHCH(R 2' ) CONR 12 R 13 , or CH(R 1' )CONHCH(R 2' )(R'). 4. The compound of claim 3, wherein R 10 = CH(R 1' )CONHCH(R 2' )COOR 11 , CH(R 1' )CONHCH(R 2' ) CONR 12 R 13 , or CH(R 1' )CONHCH(R 2' )(R'), wherein R r is H or alkyl, and R 2 is selected from the group consisting of phenyl, substituted phenyl, hetero atom-substituted phenyl, thiophenyl, cyclohexyl, cyclopentyl, cyclopropyl, piperidyl pyridyl and 2-indanyl. 5. The compound of claim 4, wherein R 1' is H. 6. The compound of claim 5, wherein R 2 = phenyl, thiophenyl, cyclohexyl, 2- indanyl, cyclopentyl, pyridyl, phenyl(4-HNS0 2 NH 2 ), R 11 is H or føtf-butyl, R 12 and R 13 are methyl, and R' is hydroxymethyl or tert-butoxymethyl. 7. The compound of claim 1 , wherein R 2 is selected from the group consisting of the following moieties: 8. The compound of claim 7 wherein R 1 = COR 5 , and R 5 is H, OH, COOR 8 , or CONR 9 R 10 . 9. The compound of claim 8 wherein V is CH. 10. The compound of claim 9 wherein Q is NR or O. 11. The compound of claim 10 wherein G is CH 2 . 12. The compound of claim 11 wherein A is O, NR, CH=CH or CH 2 . 13. The compound of claim 12 wherein E is alkyl, aryl, hereroalkyl, heteroaryl, alkyl, aryl, or cycloalkyl. 14. The compound of claim 13 wherein E is selected from the group consisting of the moieties: 15. The compound of claim 14 wherein R 3 is selected from the group consisting of the moieties: wherein R 30 = H, CH 3 or other alkyl groups; R 31 = OH, O-alkyl, NH 2 , N-alkyl; and R 32 and R 33 may be the same or different and are selected independently from H, F, CI, Br and CH 3 . 16. The compound of claim 15 wherein Z = N and R 4 = H. 17. The compound of claim 16 wherein W is C=0. 18. The compound of claim 17 wherein the moiety X-Y is selected from the group consisting of: C1-C10 alkyl, alkyl, cycloalkyl, heteroalkyl, arylalkyl, aryl, hereroaryl and alkylaryl. 19. The compound of claim 18, wherein: wherein R b is connected directly to A and R° is connected directly to W; and the moiety U 1 , U 2 , U 3 , U 4 , U 5 and U 6 form either a six membered carbon ring, or a five or six membered ring with one or more heteroatoms; R a = H, alkyl, alkoxy, hydroxy, alkylthio, halogen, nitro, cyano, carboxylic acid, ester, amide, amino, nitrile, or CF 3 ; R b is a bond, C C 6 alkyl, C 2 -C 6 alkenyl, C 2 -C 6 alkynyl, O, S, S0 2> NH, O(alkyl), S(alkyl), S0 2 (alkyl) or N(alkyl); and R c is a bond, C1-C6 alkyl, C2-C6 alkenyl, C2-C6 alkynyl, O, S, S0 2 , NH, O(alkyl), S(alkyl), S0 2 (alkyl), N(alkyl) or CH 2 -N(alkyl) with the CH 2 being linked to the aromatic ring. 20. The compound of claim 18 wherein the moiety X-Y is selected from the group consisting of the following structures: 21. A pharmaceutical composition comprising as an active ingredient a compound of claim 1. 22. The pharmaceutical composition of claim 21 for use in treating disorders associated with Hepatitis C virus. 23. The pharmaceutical composition of claim 21 additionally comprising a pharmaceutically acceptable carrier. 24. A method of treating disorders associated with the HCV protease, said method comprising administering to a patient in need of such treatment a pharmaceutical composition which composition comprises therapeutically effective amounts of a compound of claim 1. 25. The use of a compound of claim 1 for the manufacture of a medicament to treat disorders associated with the HCV protease. 26. A method of preparing a pharmaceutical composition for treating disorders associated with the HCV protease, said method comprising bringing into intimate contact a compound of claim 1 and a pharmaceutically acceptable carrier. 27. A compound exhibiting HCV protease inhibitory activity, including enantiomers, stereoisomers, rotamers and tautomers of said compound, and pharmaceutically acceptable salts or solvates of said compound, said compound being selected from the group of compounds with structures listed below: 209 210 28. A pharmaceutical composition for treating disorders associated with the HCV protease, said composition comprising therapeutically effective amount of one or more compounds in claim 27 and a pharmaceutically acceptable carrier. 29. The pharmaceutical composition of claim 28, additionally containing an antiviral agent. 30. The pharmaceutical composition of claim 28 or claim 29, still additionally containing an interferon. 31. The pharmaceutical composition of claim 30, wherein said antiviral agent is ribavirin and said interferon is α-interferon.

Inventors: ARASAPPAN ASHOK, CHAN TIN-YAU, CHEN KEVIN X, GIRIJAVALLABHAN VIYYOOR M, MADISON VINCENT S, MCKITTRICK BRIAN A, NJOROGE F GEORGE, PRONGAY ANDREW J, VENKATRAMAN SRIKANTH, ARASAPPAN, ASHOK, CHAN, TIN-YAU, CHEN, KEVIN, X., GIRIJAVALLABHAN, VIYYOOR, M., MADISON, VINCENT, S., MCKITTRICK, BRIAN, A., NJOROGE, F., GEORGE, PRONGAY, ANDREW, J., VENKATRAMAN, SRIKANTH
Applicants: SCHERING CORP, SCHERING CORPORATION
Application year: 2001
Main classification: C07D273
IPC codes: A61K31, A61K38, A61K45, A61P31, A61P43, C07D273, C07K5, C07K7