METHODS AND COMPOSITIONS FOR THE TREATMENT OF DISEASES OF THE EYE

Abstract: Methods and compositions for prophylaxis and/or treatment of diseases of the eye of a patient resulting angiogenesis in the eye using antagonists of the integrin receptors alpha v beta 3 and /or alpha v beta 5. The compositions can be nanoparticles and are administered to the eye by injection into the sclera of the eye.

Description: METHODS AND COMPOSITIONS FOR THE TREATMENT OF DISEASES OF THE EYE TECHNICAL FIELD The present invention relates generally to the field of medicine, and relates specifically to methods and compositions for the treatment of diseases of the eye using antagonists of the integin receptors α v β 3 and/or α v βs. More specifically, the invention relates to methods and compositions for the treatment of diseases of the eye using antagonists of the integrin receptors α v β 3 and/or α v βs wherein the compositions are administered by injection into the sclera of the eye. BACKGROUND Integrins are a class of cellular receptors known to bind extracellular matrix proteins, and therefore mediate cell-cell and cell-extracellular matrix interactions, referred generally to as adhasion events. Integrins receptors constitute a family of proteins across membranes with shared structural characteristics heterodimeric glycoprotein complexes formed of α and β subunits. One class of integrin receptors, the vitronectin receptor, named for its original characteristic of preferential binding to vitronectin, is known to refer to three different integrins, designated. α v βι, α v β3 and α v βs. Horton, Int. J. Exp. Pathol., 71 :741-759 (1990). α v βι binds fibronectin and vitronectin. α v β 3 binds a large variety of ligands, including fibrin, fibrinogen, laminin, thrombospondin, vitronectin, von Willebrand's factor, osteospontin and bone sialoprotein I. v βs binds vitronectin. The specific cell adhesion roles these three integrins play in the many cellular interactions in tissues is still under investigation, but it is clear that there are different integrins with different biological functions. One important recognition site in the ligand for many integrins is the arginine-glycine- aspartic acid (RGD) tripeptide sequence. RGD is found in all of the ligands identified above for the vitronectin receptor integrins. This RGD recognition site can be mimicked by polypeptides ("peptides") that contain the RGD sequence, and such RGD peptides are known inhibitors of integrin function. Integrin inhibitors containing the RGD sequence are disclosed, for example, in EP 0 770 622 A2. The compounds described inhibit in particular the interactions of β 3 - and/or βs-integrin receptors with ligands and are particularly active in the case of the integrins v β 3 , α v βs and aιιβ 3 , but also relative to α v βι, α v βe and α v βs receptors. These actions can be demonstrated, for example, according to the method described by J. W. Smith et al. in J. Biol. Chem. 265, 12267-12271 (1990). In addition, the compounds possess anti-inflammatory effects. On basis of integrin inhibitors containing the RGD sequence a multitude of antagonists without the RGD sequence have been made available. Those integrin inhibitors without RGD sequence are disclosed, for example, in WO 96/00730 A1 , WO 96/18602 A1 , WO 97/37655 A1 , WO 97/06791 A1 , WO 97/45137 A1 , WO 97/23451 A1 , WO 97/23480 A1 , WO 97/44333 A1 , WO 98/00395 A1 , WO 98/14192 A1 , WO 98/30542 A1 , WO 99/11626 A1 , WO 99/15178 A1 , WO 99/15508 A1 , WO 99/26945 A1 , WO 99/44994 A1 , WO 99/45927 A1 , WO 99/50249 A2, WO 00/03973 A1 , WO 00/09143 A1 , WO 00/09503 A1 , WO 00/33838 A . DE 1970540 A1 disclose bicyclic aromatic amino acids acting as integrin inhibitors of the α v integrin receptors, particulary of the integrins v β 3 and c. v β5.- The compounds are very particularly active as adhesion receptor antagonists for the vitronectin receptor α v β 3 . This effect can be demonstrated, for example, by the method described by J.W. Smith et al. in J. Biol. Chem. 265, 11008-1 1013 and 12267-12271 (1990). WO 00/26212 A1 discloses chromenone and chromanone derivatives acting as integrin inhibitors of the α v integrin receptors, particulary of the integrins α v β 3 and v β δ .. The compounds are also very particularly active as adhesion receptor antagonists for the vitronectin receptor α v β 3 . Integrin inhibitors have been suggested as pharmaceutically active principle in human and veterinary medicine, in particular for the prophylaxis and treatment of various disorders. Specifically suggested have been their use for the treatment and prophylaxis of the circulation, thrombosis, cardiac infarction, arteriosclerosis, inflammations, apoplexy, angina pectoris, tumor disorders, osteolytic disorders, especially osteoporosis, angiogenesis and disorders resulting from angiogenesis, for example diabetic retinopathy of the eye, macular degeneration, myopia, ocular histoplasmosis, rheumatic arthritis, osteoarthritis, rubeotic glaucoma, and also ulcerative colitis, Crohn's disease, multiple sclerosis, psoriasis and restenosis following angioplasty. Eye diseases resulting from angiogenesis are the leading cause of visual loss in America. While in case of the population of the age of over 65 visual loss is predominantly effected by age-related macular degeneration (AMD) in case of population of the age of less than 65 this is predominantly effected by diabetic retinopathy. In Wall Street Journal from March 6 th, 2000 an overview about occurence and current therapies of AMD is given. According to this AMD currently afflicts some 12 million Americans. AMD progressively destroys the macula which is responsible for central vision and color vision. In some cases, deterioration of central vision to fuzzy blur can be rapid occuring in weeks or months. Two forms of the disease exists called „atrophic" and „exudative". Although exudative AMD effects only 10% of the total AMD population, it accounts for 90% of all AMD-related blindness. Until recently, the only treatment for exudative AMD consisted of directing a powerful laser beam at the harmful blood vessels to heat and coagulate them. However, only about 15% of patients with exudative AMD have been eligible for this laser surgery. Other therapies are currently in experimental phase. In one approach, called photodynamic therapy, a low-power laser is combined with injection of light- absorbing dye. Another therapy is a more surgical approach and is called ..limited retinal translocation". In this therapy the leaky vessels are destroyed with a high- powered laser after separation and rotation of the retina from the outer wall of the eye. US 5,766,591 discribes the use of RGD-containing α v β 3 antagonists for the treatment of patients in which neovascularisation in the retinal tissue occurs. More specifically the use of said antagonists for the treatment of patients with diabetic retinopathy, macular degeneration and neovasular glaucoma is suggested. However, no examples with regard to this indications are presented. Concerning to the route of administration only general information are given. Specifically intravenous, intraperitoneal, intramuscular, intracavital and transdermal application is mentioned. In all cases v β 3 antagonists are preferred exhibiting selectivity for α v β 3 over other integrins such as α v βs. WO 97/06791 A1 discribes that α v βs antagonists can be used for inhibiting angiogenesis too. Likewise as suggested for α v β 3 antagonists in US 5,766,591 α v βs antagonists are suggested for the treatment of a patient with diabetic retinopathy, macular degeneration and neovasular glaucoma. With regard to the route of administration intravenous, intraocular, intrasynovial, intramuscular, transdermal and oral application is specifically mentioned. WO 00/07565 A1 discribes a method for application of pharmaceutically active substances to the eye via intrascleral injection into the scleral layer. The whole disclosure of WO 00/07565 A1 is incorporated to the present application by reference. As active substances a multitude of active substances is mentioned in WO 00/07565 A1 including integrin blockers. However, the term integrin blocker is silent with regard to the receptor type and refer to all substances acting as inhibitor on anyone of the large class of heterodimeric receptors formed from α and β subunits. Moreover, no examples for integrin blockers are given. DESCIPTION OF THE INVENTION It has been found that inhibitors of α v β3 and/or α βs integrin receptors have particularly useful pharmacological and physicochemical properties combined with good tolerability, as, in particular, they can be used for prophylaxis and treatment of diseases of the eye of a patient resulting from angiogenesis in the eye by injecting the inhibitor into the scleral layer of the eye. Accordingly, the invention is directed to a method for prophylaxis and/or treatment of diseases of the eye of a patient resulting from angiogenesis in the eye comprising injecting into the scleral layer of the eye of said patient a composition comprising a therapeutically effective amount of an α v β 3 and/or α v βs inhibitor sufficient to inhibit angiogenesis of the eye whereby injecting occurs through the location of the exterior surface of the sclera that overlies retinal tissue. A therapeutically effective amount is an amount of inhibitor sufficient to produce a measureable inhibition of angiogenesis in the tissue of the eye when injected into the scleral layer. In general, this is the case when the α v β 3 and/or α v βs inhibitor is used in an amount from about 0.5 μg to about 5 mg. The method of invention is especially usable for prophylaxis and/or treatment of diabetic retinopathy, macular degeneration, myopia and histoplasmosis. In a preferred embodiment of the invention polypeptides containing the amino acid sequence RGD are used as α v β 3 and/or α v βs inhibitors in the method for prophylaxis and/or treatment of eye diseases. As mentioned above, RGD is the peptide sequence Arg-Gly-Asp (arginine-glycine-aspartic acid) occuring in natural ligands of integrins like fibronectin or vitronectin. Solvable RGD containing linear or cyclic peptides are able to inhibit interactions of this integrins with their corresponding natural ligands. The abbreviations for the amino acid residues used hereinafter are shown in the following table: Ala A alanine Arg R arginine Asp D aspartic acid D-homoPhe D-homo-phenylalanine D-Nal D-3-(2-naphthyl)alanine D-Phe D-phenylalanine D-Phg D-phenylglycine D-Trp D-tryptophan D-Tyr D-tyrosine Gly G glycine 4-Hal-Phe 4-halo-phenylalanine homoPhe homo-phenylalanine lie 1 isoleucine Leu L leucine Nal 3-(2-naphthyl)alanine Nle norleucine Phe F phenylalanine Phg phenylglycine Trp W tryptophan Tyr Y tyrosine Val V valine. Particularly preferred as α v β 3 and/or α v βs inhibitors to be used in the method for prophylaxis and/or treatment of eye diseases are compounds of formula I cyclo-(Arg-Gly-Asp-D-(A) n E) I, in which D is D-Phe, Phe, D-Trp, Trp, D-Tyr, Tyr, D-homoPhe, homoPhe, D-Nal, Nal, D-Phg, Phg or 4-Hal-Phe (D or L form), in which Hal is F, Cl, Br, I, E is Val, Gly, Ala, Leu, lie or Nle, A is alkyl having 1-18 carbon atoms and n is 0 or 1 and also their physiologically acceptable salts. In formula I alkyl is preferably methyl, ethyl, isopropyl, n-butyl, sec-butyl or tert-butyl. More particular preferred polypeptides are used as α v β 3 and/or α v βs inhibitors in the method of the invention that can be expressed by the subformula la, which otherwise corresponds to the formula I but in which D is D-Phe and E is Gly, Ala, Val, Leu, lie or Nle. Furthermore, particular preference is given to the use of all physiologically compatible salts of the compounds which come under the subformula la. Most preferred as active compound in said method are cyclo-(Arg-Gly-Asp-DPhe- Val) and cyclo-(Arg-Gly-Asp-DPhe-NMeVal). This RGD-containing peptides described by formula I as well as the peptides specifically mentioned hereinbefore are disclosed in EP 0 770 622 A2, the disclosure of which is hereby incorporated to the present application by reference. Accordingly, the meaning of the substituents of formula I resp. subformula la are the same as defined for the substituents of subformula la resp. subformula lb as disclosed on page 5, line 24 to line 32 resp. page 5, line 33 to line 41 in EP 0 770 662 A2. It has been found that inhibitors of α v β3 and/or v βs integrin receptors which are no polypeptides and do not contain the RGD sequence can also be used for prophylaxis and treatment of diseases of the eye of a patient resulting from angiogenesis in the eye by injecting the inhibitor into the scleral layer of the eye. Therefore, in one further preferred embodiment of the method of invention the α v β 3 and/or α v βs inhibitors to be used in the method for prophylaxis or treatment of eye diseases are compounds of formula II wherein R 1 is H, alkyl having 1-6 C atoms or benzyl, R 2 is R 10 , CO-R 0 , COOR 6 , COOR 10 , SO 2 R 6 or SO 2 R 10 , R 3 is H, Hal, OA, NHR 10 , N(R 10 ) 2l -NH-acyl, -O-acyl, CN, NO 2 , OR 10 , SR 10 , R 2 or CONHR 10 , R 4 is H, =O, =S, d-Ce-alkyl or acyl, R 5 is NH 2 , H 2 N-C(=NH) or H 2 N-(C=NH)-NH, where the primary amino groups can also be provided with conventional amino protective groups or can be mono-, di- or trisubstituted by R 10 , CO-R 10 , COOR 10 or SO 2 R 10 ,or R 6 , R 7 , R 8 are each independently of one another absent or H, R 7 and R 8 together are also a bond, X, Y are each independently of one another =N-, -N-, O, S, -CH 2 - or =C-, with the proviso that at least one of the two definitions X, Y is =N-, -N-, O or S, W, Z are each independently of one another absent, O, S, NR 1 , C(=O), CONH, NHCO, C(=S)NH, NHC(=S), C(=S), SO 2 NH, NHSO 2 or CA=CA, R 6 is a mono- or binuclear heterocycle which has 1 to 4 N, O and/or S atoms and can be unsubstituted or mono-, di- or trisubstituted by Hal, A, -CO-A, OH, CN, COOH, COOA, CONH 2l NO 2> =NH or =O, R 9 is H, Hal, OA, NHA, NAA', NHacyl, Oacyl, CN, NO 2 , SA, SOA, SO 2 A, SO 2 Ar or SO 3 H, R 10 is H, A, Ar or aralkyl having 7-14 C atoms, R 11 is H or alkyl having 1 -6 C atoms, A, A' are each independently of one another H or unsubstituted or mono-, di- or tri-R 9 -substituted alkyl or cycloalkyl, each of which has 1-15 C atoms and in which one, two or three methylene groups can be replaced by N, O and/or S, Ar is unsubstituted or mono-, di- or tri-A- and/or R 9 -substituted mono- or binuclear aromatic ring system having 0, 1 , 2, 3 or 4 N, O and/or S atoms, Hal is F, Cl, Br or I and m, n are each independently of one another 0, 1 , 2, 3 or 4, and the physiologically acceptable salts thereof. Particularly preferred α v β 3 and/or α v βs inhibitors are used in the method of invention that can be expressed by the subformulae Ila to llg, which otherwise corresponds to the formula II but in which in Ila) R 1 is H or alkyl with 1-6 C atoms, R 2 is R 10 , CO-R 10 , COOR 10 or SO 2 R 10 , R 3 is H, R 4 is H or =O, R 5 is H 2 N-C(=NH) or H 2 N-C(=NH)-NH, W, Z are each independently of one another absent, C(=O), NH, CONH or NHCO, X is -NH-, O or -CH 2 -, Y is NH or O, R 10 is H, A or benzyl, R 11 is H, A is unsubstituted alkyl or cycloalkyl with 1-15 C atoms and m, n are each independently of one another 0, 1 or 2; in lib) R 1 is H or alkyl with 1-6 C atoms, R 2 is R 10 , CO-R 10 , COOR 10 or SO 2 R 10 , R 3 is H, R 4 is H or =O, R 5 is R 6 , W, Z are each independently of one another absent, C(=O), NH, CONH or NHCO, X is -NH-, O or -CH 2 -, Y is NH or O, R 6 is a mono- or binuclear heterocycle which has 1-4 N, O and/or S atoms and which can be unsubstituted or mono-, di- or trisubstituted by Hal, A, -CO-A, OH, CN, COOH, COOA, CONH 2 , NO 2 , =NH or =O, R 10 is H, A or benzyl, R 11 is H, A is unsubstituted alkyl or cycloalkyl with 1-15 C atoms and m, n are each independently of one another 0, 1 or 2; in lie) R 1 is H or alkyl with 1-6 C atoms, R 2 is R 10 , CO-R 10 , COOR 10 or SO 2 R 10 , R 3 is H, R 4 is H or =O, R 5 is H 2 N-C(=NH) or H 2 N-C(=NH)-NH, W, Z are each independently of one another absent, C(=O), NH, CONH or NHCO, X is -NH-, O or -CH 2 -, Y is NH or O, A is alkyl with 1-6 C atoms, R 10 is H, alkyl with 1-6 C atoms, camphor-10-yl or benzyl, R 11 is H, m, n are each independently of one another 0, 1 or 2; in lid) R is H or alkyl with 1-6 C atoms, R 2 is R 10 , CO-R 10 , COOR 10 or SO 2 R 10 , R 3 is H, R 4 is H or =O, R 5 is R 6 , W, Z are each independently of one another absent, C(=O), NH, CONH or NHCO, X is =NH-, O or -CH 2 -, Y is NH or O, R 6 is a mono- or binuclear heterocycle which has 1-4 N, O and/or S atoms and which can be unsubstituted or mono-, di- or trisubstituted by Hal, A, -CO-A, OH, CN, COOH, COOA, CONH 2 , NO 2 , =NH or =O, R 10 is H, alkyl with 1-4 C atoms, camphor-10-yl or benzyl, R 11 is H, A is unsubstituted alkyl with 1-6 C atoms and m, n are each independently of one another 0, 1 or 2; in lie) R 1 is H or alkyl with 1-6 C atoms, R 2 is R 10 , CO-R 10 , COOR 10 or SO 2 R 10 , R 3 is H, R 4 is H or =O, R 5 is R 6 , W, Z are each independently of one another absent, C(=O), NH, CONH or NHCO, X is -NH-, O or -CH 2 -, Y is NH or O, R 6 is 1H-imidazol-2-yl, thiazol-2-yl, 1 H-benzimidazol-2- yl, 2H-pyrazol-2-yl, 1 H-tetrazol-5-yI, 2-imino- imidazolidin-4-on-5-yl, 1 -A- ,5-dihydro-imidazol-4- on-2-yl, pyrimidin-2-yl or 1 ,4,5,6-tetrahydro- pyrimidin-2-yl, R 10 is H, alkyl with 1-4 C atoms, camphor-10-yl or benzyl, R 11 is H, A is unsubstituted alkyl with 1-6 C atoms and m, n are each independently of one another 0, 1 or 2; in I If) R 1 is H or alkyl with 1-6 C atoms, R 2 is R 10 , CO-R 10 , COOR 10 or SO 2 R 10 , R 3 is H, R 4 is H or =O, R 5 is H 2 N-C(=NH) or H 2 N-C(=NH)-NH, W, Z are each independently of one another absent, C(=O), NH, CONH or NHCO, X is -NH-, O or -CH 2 -, Y is NH or O, R 10 is Ar, R 11 is H, A is unsubstituted alkyl or cycloalkyl with 1-15 C atoms and m, n are each independently of one another 0, 1 or 2; in llg) R 1 is H or alkyl with 1-6 C atoms, R 2 is R 10 , CO-R 10 , COOR 10 or SO 2 R 10 , R 3 is H, R 4 is H or =O, R 5 is R 6 , W, Z are each independently of one another absent, C(=O), NH, CONH or NHCO, X is -NH-, O or -CH 2 -, Y is NH or O, R 6 is a mono- or binuclear heterocycle which has 1-4 N, O and/or S atoms and which can be unsubstituted or mono-, di- or trisubstituted by Hal, A, -CO-A, OH, CN, COOH, COOA, CONH 2 , NO 2) =NH or =O, R 10 is Ar, R 1 is H, A is unsubstituted alkyl or cycloalkyl with 1-15 C. atoms and m, n are each independently of one another 0, 1 or 2. The compounds of formula II and subformulae Ila to llg have been disclosed in DE 197 05 450 A1 , the whole disclosure of which is hereby incorporated to the present application by reference. Accordingly, the substituents of formula II resp. subformulae Ila to llg have the same meaning as defined for the substituents of formula I resp. subformulae la to Ig as disclosed on page 2, lines 3 to 43 resp. page 5, line 58 to page 7, line 30 of DE 197 05 450 A1. The definitions for the substituents are given on page 4, line 35 to page 5, line 56 of DE 197 05 450 A1. More particularly preferred one of the following α v β 3 and/or α v βs inhibitors is used in the method of the present invention: (2S)-2-[(R)-camphor-10-sulfonamido]-3-{3,4-dihydro-2-(3-guanidino-propyl)- (2R)-2H-1 ,4-benzoxazin-3-on-6-yl}propionic acid; (2S)-2-benzyloxycarboxamido-3-(2-guanidinomethyl-1 ,4-benzodioxan-6- yl)propionic acid; (2S)-2-tert-butyloxycarboxamido-3-[3,4-dihydro-2-(2-guanidino-2-oxoethyl)- 2H-1 ,4-benzoxazin-3-on-6-yl]propionic acid; (2S)-2-benzyloxycarboxamido-3-(2-guanidinoacet-amidomethyl-1 ,4- benzodioxan-6-yl)propionic acid; (2S)-2-tert-butyloxycarboxamido-3-{3,4-dihydro-2-[N-(2-imidazolyl)- carbamoylmethyl]-2H-1 ,4-benzox-azin-3-on-6-yl)propionic acid; (2S)-2-tert-butyloxycarboxamido-3-{3,4-dihydro-2-[N-(2-benzimidazoiyl)- carbamoylmethyl]-2H-1 ,4-benzoxazin-3-on-6-yl)propionic acid; (2S)-2-tert-butyloxycarboxamido-3-{3,4-dihydro-2-[2-(2-imino-4- oxoimidazolidin-5-yl)ethyl]-2H-1 ,4-benzoxazin-3-on-6-yl}propionic acid; (2S)-2-(2,2-dimethylpropyloxycarboxamido)-3-{3,4-dihydro-2-[N-(2- imidazolyl)carbamoylethyl]-(2S)-2H-1 ,4-benzoxazin-3-on-6-yl}propionic acid; (2S)-2-[(R)-camphorsulfonamido]-3-{3,4-dihydro-2-[N-(2- benzimidazolyl)carbamoylmethyl]-2H-1 ,4-benzoxazin-3-on-6-yl)propionic acid and their physiologically acceptable salts. Most preferred are (2S)-2-(2,2-dimethylpropyloxycarboxamido)-3-{3,4-dihydro-2-[N-(2- imidazolyl)carbamoyl-ethyl]-(2S)-2H-1 ,4-benzoxazin-3-on-6-yl}propionic acid and (2S)-2-[(R)-camphorsulfonamido]-3-{3,4-dihydro-2-[N-(2-benzimidazolyl)- carbamoylmethyl]-2H-1 ,4-benzoxazin-3-on-6-yl)propionic acid In one further preferred embodiment of the method of invention the α v β3 and/or α v βs inhibitors to be used in the method for prophylaxis or treatment of eye diseases are compounds of formula III in which R 1 is CH 2 OR 10 , COOR 10 , CONHR 0 or CON(R 12 ) 2 , R is R 10 , CO-R 10 , CO-R 6 , COOR 6 , COOR 10 , SO 2 R 6 , SO 2 R 10 , CONHR 6 , CON(R 6 ) 2 , CONHR 10 or CON(R 12 ) 2 , R d is H, Hal, NHR 10 , N(R 12 ) 2 , NH-acyl, -O-acyl, CN, NO 2 , OR 10 , SR 10 , SO 2 R 10 , SO 3 R 10 , COOR 10 , CONHR 6 , CON(R 6 ) 2 , CONHR 10 or CON(R 12 ) 2 , R 4 is H, A, Ar or aralkylene having 7-14 C atoms, R 5 is NH 2 , H 2 N-C(=NH) or H 2 N-(C=NH)-NH, where the primary amino groups can also be provided with conventional amino protective groups, or can be mono- di- or trisubstituted by R 0 , CO-R 10 , COOR 10 or SO 2 R 10 , or R 6 -NH-, R e is a mono- or binuclear heterocycle having 1 to 4 N, O and/or S atoms, which can be unsubstituted or mono-, di- or trisubstituted by Hal, A, -CO-A, OH, CN, COOH, COOA, CONH 2 , NO 2 , =NH or =O, R 7 , R 8 in each case independently of one another is absent or is H, R 7 and R 8 together are also a bond, Z is absent, O, S, NH, NR 1 , C(=O), CONH, NHCO, C(=S)NH, NHC(=S), C(=S), SO 2 NH, NHSO 2 or CA=CA\ R 9 is H, Hal, OR 11 , NH 2 , NHR 12 , N(R 12 ) 2 , NHAcyl, OAcyl, CN, NO 2 , SR 11 , SOR 12 , SO 2 R 12 or SO 3 H, R 10 is H, A, Ar or aralkylene having 7-14 C atoms, R 11 is H or alkyl with 1 -6 C atoms, R 12 is alkyl having 1-6 C atoms, A is H or alkyl having 1 -15 C atoms or cycloalkyl having 3-15 C atoms, which is unsubstituted or is mono-, di- or trisubstituted by R 9 and in which one, two or three methylene groups can also be replaced by N, O and/or S, Ar is a mono- or binuclear aromatic ring system having 0, 1 , 2, 3 or 4 N, O and/or S atoms, which is unsubstituted or mono-, di- or trisubstituted by A and/or R 9 , Hal is F, Cl, Br or l, m, n in each case independently of one another are 0, 1 , 2, 3 or 4, and their physiologically acceptable salts and solvates. In this embodiment of the method of the present invention particularly preferred α v β3 and/or α v βs inhibitors are used that can be expressed by the subformulae Ilia to llln, which otherwise correspond to formula III but in which in Ilia) R 3 is H; in 1Mb) R 3 is H and R 2 is COOR 10 or SO 2 R 10 ; in lllc) R 3 is H, R 2 is COOR 10 or SO 2 R 10 R 10 is H, A, Ar or aralkylen in Hid) m is O; in llle) m is 0 and R 3 is H; in 11 If) R 3 is H, R 2 is COOR 10 or SO 2 R 10 and m is O; in lllg) R 3 is H, R 2 is COOR 10 or SO 2 R 10 and R 10 is H, A, Ar or aralkylene with 7-14 C atoms and m is O; in lllh) is H, R 2 is COOR 10 or SO 2 R 10 and R 10 is H, A, Ar or aralkylene having 7-14 C atoms and A is H or unsubstituted alkyl having 1-15 C atoms or cycloalkyl having 3-15 C atoms; Ar is phenyl or naphthyl and m is O; in UN) R° is a mono- or binuclear heterocycle having 1 to 4 N atoms, which can be unsubstituted or mono-, di- or trisubstituted by Hal, A, -CO-A, OH, CN, COOH, COOA, CONH 2 , NO 2 , =NH or =O, in R° is H, R 2 is COOR 10 or SO 2 R 10 and R 10 is H, A, Ar or aralkylene having 7-14 C atoms and m is O; R 6 is a mono- or binuclear heterocycle having 1 to 4 N atoms, which can be unsubstituted or mono-, di- or trisubstituted by Hal, A, -CO-A, OH, CN, COOH, COOA, CONH 2 , NO 2 , =NH or =O; in lllk) is absent; in III!) z is absent and R 3 is H; in lllm) Z is absent, R 3 is H and R 2 is COOR 10 or SO 2 R 10 ; in llln) Z is absent, R 3 is H, R 4 is H, R 2 is COOR 10 or SO 2 R 10 ; R 10 is H, A, Ar or aralkylene having 7-14 C atoms, R 6 is a mono- or binuclear heterocycle having 1 to 4 N atoms, which can be unsubstituted or mono-, di- or trisubstituted by Hal, A, -CO-A, OH, CN, COOH, COOA, CONH 2 , NO 2 , =NH or =O, A is H or unsubstituted alkyl having 1-6 C atoms, Ar is phenyl or naphthyl and m is 0. The compounds of formula 111 and subformulae Ilia to llln have been disclosed in WO 00/26212 A1 , the whole disclosure of which is incorporated to the present application by reference. Accordingly, the substituents of formula III resp. subformulae Ilia to llln have the same meaning as defined for the substituents of formula I resp. subformulae la to In as disclosed on page 1 , line 5 to page 2, line 31 resp. page 13, line 20 to page 15, line 6 of WO 00/26212 A1. The definitions for the substituents are given on page 8, line 18 to page 13, line 10 of WO 00/26212 A1. More particularly preferred one of the following α v β3 and/or α v βs inhibitors is used in this embodiment of the method of the present invention: (2S)-3-[2-(3-aminopropyI)-4-oxo-4H-chromen-6-yl]-2-(2,2-dimethylpropoxy- carboxamido)-propionic acid; (2S)-3-{2-[3-(1H-imidazol-2-ylamino)propyl]-4-oxo-4/-/-chromen-6-yl}-2-(2,2- dimethylpropoxycarboxamido)propionic acid; (2S)-3-{2-[3-(1H-imidazol-2-ylamino)propyI]-4-oxochroman-6-yl}-2-(2,2-dimethyl- propoxycarboxamido)propionic acid; (2S)-3-{2-[3-(pyridin-2-ylamino)propyl]-4-oxo-4H-chromen-6-yl}-2-(2,2-dimethyl- propoxycarboxamido)propionic acid; (2S)-3-{2-[3-(1H-benzimidazol-2-ylamino)propyl]-4-oxo-4H-chromen-6-yl}-2-(2,2- dimethylpropoxycarboxamido)propionic acid; (2S)-3-{2-[3-(1 H-imidazol-2-ylamino)propyl]-4-oxo-4H-chromen-6-yl}-2-butyl- sulfonamidopropionic acid; (2S)-3-{2-[3-(pyridin-2-ylamino)propyl]-4-oxo-4H-chromen-6-yl}-2-(2,4,6-trimethyl- phenyl)sulfonamidopropionic acid or their physiologically acceptable salts and solvates. Most preferred are (2S)-3-{2-[3-(1 H-imidazol-2-ylamino)propyl]-4-oxo-4H-chromen-6-yl}-2- butylsulfonamidopropionic acid and (2S)-3-{2-[3-(pyridin-2-ylamino)propyl]-4-oxo-4H-chromen-6-yl}-2-(2,4,6- trimethylphenyl)sulfonamidopropionic acid. In one further preferred embodiment of the method of invention the α v β 3 and/or α v βs inhibitors to be used in the method for prophylaxis or treatment of eye diseases are compounds of formula IV wherein A and B are each independently of one another O, S, NH, NR 7 , CO, CONH, NHCO or directly bond, X is alkylene having 1-2 C atoms, which is unsubstituted or monosubstituted by R 4 or R 5 or a direct bond, R 1 is H, Z or -(CH 2 ) 0 -Ar, R 2 is H, R 7 or -C(O)Z, R° is NHR 6 , -NR 6 -C(=NR 6 )-NHR 6 , -C(=NR 6 )-NHR 6 , -NR 6 -C(=NR 9 )- NHR 6 , -C(=NR 9 )-NHR 6 or Het 1 , R 4 or R 5 are each indipendently of one another H, oxo, R 7 , -(CH 2 ) 0 -Ar, -C(O)-(CH 2 ) 0 -Ar, -C(O)-(CH 2 ) 0 -R 7 , -C(O)-(CH 2 ) 0 -Het, Het, NHR 6 , NHAr, NH-Het, OR 7 , OAr, OR 6 or O-Het, R α is H, -C(O)R 7 , -C(O)-Ar, R 7 , COOR 7 , COO-(CH 2 ) 0 -Ar, SO 2 -Ar, SO 2 R 7 or SO 2 -Het, R 7 is alkyl having 1 to 10 C atoms or cycloalkyl having 1 to 10 C atoms, R a is Hal, NO 2 , CN, Z, -(CH 2 ) 0 -Ar, COOR 1 , OR 1 , CF 3 , OCF 3 , SO 2 R 1 , NHR 1 , N(R 1 ) 2 , NH-C(O)R 1 , NHCOOR 1 or C(O)R 1 , R 9 is CN or NO 2 , Z is alkyl having 1 to 6 C atoms, Ar is aryl, which is unsubstituted or substituted by R 6, Hal is F, Cl, Br or l, Het is unsaturated, partly of fully saturated mono- or bicyclic heterocyclic ring system having 5 to 10 atoms, which can contain 1 or 2 N atoms and/or 1 or 2 S or O atoms and wherein the heterocyclic ring system can be mono or disubstituted by R 8 , Het 1 is a mono or bicyclic aromatic heterocyclic ring system having 1 to 4 N atoms, which can be unsubstituted or mono or disubstituted by Hal, R 7 , OR 7 , CN, NHZ or NO 2 , n is 0, 1 or 2 m is O, 1 , 2, 3, 4, 5 or 6, o is 0, 1 or 2 as well as their physiologically acceptable salts and solvates. In this embodiment of the method of invention particularly preferred α v β3 and/or α v βs inhibitors are used that can be expressed by the subformulae IVa to IVi, which otherwise correspond to formula IV but in which in IVa X is a direct bond in lVb X is a direct bond, R 2 is H, R 5 is H and R 4 is Ar inlVc X is a direct bond, R 5 is H and R 4 is Ar or Het; inlVd X is a direct bond, R 5 isH, B isO, A isNH, n isO, m is 3 or 4, R 3 is Het and R 4 isAr inlVe X is a direct bond, R 5 isH, B isO, A isNH, n isO, m is 3 or 4 and R J is Het inlVf is methylene, which is unsubstituted or substituted byAr, R 2 isH, R 5 is H oder Ar and R 4 is oxo inlVg X is methylene, R 3 -(CH 2 ) n -A-(CH 2 ) m -B IVg inlVh X is methylene, R 4 is H or Ar, R 5 is H or Ar and R 2 is H; in IVi X is methylene, R 4 is H or Ar, R 5 is H or Ar, B is O, A is NH, n is O, m is 3 or 4 R 3 is Het and R 2 is H More particularly preferred the α v β 3 and/or α v βs inhibitor according to formula IV to be used in the method of the present invention is: 3-phenyl-3-{6-[3-(pyridine-2-ylamino)-propoxy]-1H-indole-3-yl}-propionic acid; 3-phenyl-3-{6-[4-(pyridine-2-ylamino)-butoxy]-1 H-indole-3-yl}-propionic acid; 3-phenyl-3-{5-[4-(pyridine-2-yIamino)-butoxy]-1 H-indole-3-yl}-propionic acid; 3-phenyl-3-{5-[3-(pyridine-2-ylamino)-propoxy]-1H-indole-3-yl}-propionic acid; 3-phenyl-3-[6-(pyridine-2-yl-amidocarboxymethoxy)-indole-3-yl]-propionic acid; 3-phenyl-3-[6-(benzimidazole-2-yl-amidocarboxymethoxy)-indole-3-yl]-propionic acid or 3-phenyl-3-[6-(imidazole-2-yl-amidocarboxymethoxy)-indole-3-yl]-propionic acid as well as their physiologically acceptable salts and solvates. Most preferred the α v β 3 and/or α v β 5 inhibitor according to formula IV to be used in the method of the present invention is 3-phenyl-3-{6-[3-(pyridine-2-ylamino)-propoxy]-1 H-indole-3-yl}-propionic acid. This compounds as well as the compounds of formula IV and subformulae IVa to IVi are disclosed in copending german patent application no. 100 06 139.7, the whole disclosure of which is hereby incorporated to the present application by reference. Accordingly, the substituents of formula IV and subformulae IVa to IVi have the same meaning as defined for the substituents of formula I resp. subformulae la to li as disclosed on page 1 , line 3 to page 2, line 13 resp. page 17, line 4 to page 20, line 9 of german patent application no. 100 06 139.7. The definitions for the substituents are given on page 9, line 6 to page 16, line 28 of german patent application no. 100 06 139.7. The particular suitability of the compounds as described hereinbefore for using in the method of treatment of eye diseases was experimentally confirmed for some representative compounds. Inhibition of angiogenesis after intrascieral application of the compounds can be demonstrated by quantification of neovascularisation in the eye after stimulation of angiogenesis and subsequent intrascieral application of the α v β 3 and/or α v βs inhibitor. One model suitable for demonstrating the inhibiting effect of v β3 and/or α v βs inhibitor on angiogenesis is, for example, the rabbit corneal micropocket model described by Shaffer R.W. et al., in: Molecular, Cellular, and Clinical Aspects of Angiogenesis, Maragoudakis E. (ed.), Plenum Press, New York, 241ff. (1996). In this model angiogenesis is stimulated by implantation of Hydron pellets containing an angiogenesis stimulating cytokine like, for example, fibroblast growth factor (FGF) or vascular endothelial growth factor (VEGF) into the cornea. After implantation the active compound to be tested is administered by paralimbal intrascieral injection. Effect on neovascularisation is measured after predetermined time intervals by visual examination using a microscope, photographing and computer-assisted quantification of photographs. As an alternative to application of cytokine induced angiogenesis, induction of angiogenesis can also be performed by laser photocoagulation, as, for example, described by Murata T. et al., IOVS, 41 , 2309ff. (2000). It is a further object of the invention to provide a composition suitable for the method for prophylaxis and treatment of diseases of the eye of a patient resulting from angiogenesis comprising injecting into the scleral layer of the eye of said patient a composition comprising a therapeutically effective amount of an α v β 3 and/or α v β 5 inhibitor sufficient to inhibit angiogenesis of the eye. The formulation used for administration of the compound into the scleral layer of the eye can be any form suitable for application into the sclera by injection through a cannula with small diameter suitable for injection into the scleral layer. Examples for injectable application forms are solutions, suspensions or colloidal suspensions. The sclera is a thin avascular layer, comprised of highly ordered collagen network surrounding most of vertebrate eye. Since the sclera is avascular it can be utilized as a natural storage depot from which injected material cannot rapidly removed or cleared from the eye. Depending from the application form the active compound liberates in an immediate or a sustained release manner. A sustained release formulation is preferred because the injection frequency can be further reduced. One possibility to achieve sustained release kinetics is embedding or encapsulating the active compound into nanoparticles. Nanoparticles can be administrated as powder, as powder mixture with added excipients or as suspensions. Colloidal suspensions of nanoparticles are preferred because they can easily be administrated through a cannula with small diameter. Nanoparticles are particles with a diameter from about 5 nm to up to about 1000 nm. The term ..nanoparticles" as it is used hereinafter refers to particles formed by a polymeric matrix in which the active compound is dispersed, also known as „nanospheres", and also refers to nanoparticles which are composed of a core containing the active compound which is surrounded by a polymeric membrane, also known as „nanocapsules". For administration into the sclera of the eye nanoparticles are preferred having a diameter from about 50 nm to about 500 nm, in particular from about 100 nm to about 200 nm. Nanoparticles can be prepared by in situ polymerization of dispersed monomers or by using preformed polymers. Since polymers prepared in situ are often not biodegradable and/or contain toxicological serious byproducts nanoparticles from preformed polymers are preferred. Nanoparticles from preformed polymers can be prepared by different techniques, i.e. by emulsion evaporation, solvent displacement, salting-out and by emulsification diffusion. Emulsion evaporation is the classical technique for preparation of nanoparticles from preformed polymers. According to this technique, the polymer and the active compounds are dissolved in a water-immiscible organic solvent, which is emulsified in an aqueous solution. The crude emulsion is then exposed to a high-energy source such as ultrasonic devices or passed through high pressure homogenizers or microfluidizers to reduce the particle size. Subsequently the organic solvent is removed by heat and/or vacuum resulting in formation of the nanoparticles with a diameter of about 100 nm to about 300 nm. Usually, methylene chloride and chloroform are used as organic solvent because of their water insolubility, good solubilizing properties, easy emulsification and high volatility. These solvents are, however, critical in view of their physiological tolerability. Moreover, the high shear force needed for particle size reduction can lead to damage of polymer and/or the active compound. The solvent displacement process was firstly described in EP 0 274 961 A1. In this process the active compound and the polymer are dissolved in an organic solvent which is miscible with water in all proportions. This solution is introduced in an aqueous solution containing a stabilizer under gentle agitation resulting in spontaneous formation of nanoparticles. Examples for suitable organic solvents and stabilizer are acetone or ethanol resp. polyvinyl alcohol. Advantageously chlorinated solvents and shear stress can be avoided. The mechanism of formation of nanoparticles has been explained by interfacial turbulence generated during solvent displacement (Fessi H. et al., Int. J. Pharm. 55 (1989) R1-R4). Recently, a solvent displacement technique was disclosed by WO 97/03657 A1, in which the organic solvent containing the active compound and the polymer is introduced into the aqueous solution without agitation. The salting-out technique was firstly described in WO 88/08011 A1. In this technique a solution of a water-insoluble polymer and an active compound in a water-soluble organic solvent, especially acetone, is mixed with a concentrated aqueous viscous solution or gel containing a colloidal stabilizer and a salting-out agent. To the resulting oil-in-water emulsion water is added in a quantity sufficient to diffuse into the aqueous phase and to induce rapid diffusion of the organic solvent into the aqueous phase leading to interfaciale turbulence and formation of nanoparticles. The organic solvent and the salting-out agent remaining in the suspension of nanoparticles are subsequently eliminated by repeated washing with water. Alternatively, the solvent and salting-out agent can be eliminated by cross-flow filtration. In emulsification-diffusion process the polymer is dissolved in a water-saturated partially water-soluble organic solvent. This solution is mixed with an aqueous solution containing a stabilizer resulting in an oil-in-water emulsion. To this emulsion water is added causing the solvent to diffuse into the aqueous external phase accompanied with formation of nanoparticles. During particle formation each emulsion droplet leads to several nanoparticle. As this phenomenon cannot be fully explained by convection effect caused by interfacial turbulence, it has been proposed that diffusion of organic solvent from the droplets of the crude emulsion carries molecules of active compound and polymer phase into the aqueous phase resulting in supersaturated local regions, from which the polymer aggregates in the form of nanoparticles (Quintanar-Guerrero D. et al. Colloid. Polym. Sci. 275 (1997) 640-647). Advantageously, pharmaceutically acceptable solvents like propylene carbonate or ethyl acetate can be used as organic solvents. With the methods described above nanoparticles can be formed with various types of polymers. For use in the method of the present invention, which involves injection of the formulation into the sclera of the eye, nanoparticles made from biocompatible polymers are preferred. The term „biocompatible" refers to material which, after introducing in a biological environment, have no serious effects to the biological environment. From biocompatible polymers those polymers are especially preferred which are also biodegradable. The term ..biodegradable" refers to material which, after introducing in a biological environment, is enzymatically or chemically degraded into smaller molecules which can be eliminated subsequently. Biodegradable polymers are well known by the person skilled in the art. Examples are polyesters from hydroxycarboxylic acids such as poly(lactic acid) (PLA), poly(glycolic acid) (PGA), polycaprolactone (PCL), copolymers of lactic acid and glycolic acid (PLGA), copolymers of lactic acid and caprolactone, polyepsilon caprolactone, polyhyroxy butyric acid and poly(ortho)esters, polyurethanes, polyan hydrides, polyacetals, polydihydropyrans, polycyanoacrylates, natural polymers such as alginate and other polysaccharides including dextran and cellulose, collagen and albumin. Liposomes are a further drug delivery system which is easily injectable. Accordingly, in the method of invention the active compounds can also be administered into the sclera of the eye in the form of a liposome delivery system. Liposomes are well- known by a person skilled in the art. Liposomes can be formed from a variety of phospholipids, such as cholesterol, stearylamine of phosphatidylcholines. Liposomes being usable for the method of invention encompass all types of liposomes including, but not limited to, small uniiamellar vesicles, large unilamellar vesicles and multilamellar vesicles. Example The effect of intrascieral application of an α v β 3 and/or α v β 5 inhibitor was examined in rabbit corneal micropocket model as described by Shaffer R.W. (see above). As an example for an α v β 3 and/or α v β 5 inhibitor (2S)-2-(2,2-dimethylpropyloxycarboxamido)- 3-{3,4-dihydro-2-[N-(2-imidazolyl)carbamoylethyl]-(2S)-2H-1 ,4-benzoxazin-3-on-6- yl}propionic acid was used in the experiment. For induction of angiogenesis Hydron pellets containing basic fibroblast growth factor (bFGF) were used. Preparation of bFGF containing implants was performed by casting Hydron [poly(hydroxyethyl)methacrylate] in specially prepared Teflon pegs that have a 5 mm core drilled into their surfaces. Approximately 12 μl of casting material was placed into each peg and polymerized overnight in a sterile hood, then sterilized by ultraviolet irradiation. The experiment consisted of 12 animals; in each eye of the animals one individual pellet was implanted into a surgically created "pocket" in the mid stroma of the rabbit cornea. The surgical procedure was done under sterile technique using a Wild model M691 operating microscope equipped with a beamsplitter and camera for photographically recording individual corneas. A 69 Beaver blade was used to create a 3 mm by 5 mm "pocket" to a depth of half the corneal thickness. The stroma was dissected peripherally using an spatula and the pellet implanted with its peripheral margin 2 mm from limbus. Immediately after implantation of bFGF-containing Hydron pellets 6 of the 12 animals received in each eye 100 μl of a drug solution consisting of 2.0 mg/ml (2S)-2-(2,2-dimethylpropyloxycarboxamido)-3-{3,4-dihydro-2-[N-(2- imidazolyl)carbamoylethyl]-(2S)-2H-1 ,4-benzoxazin-3-on-6-yl}propionic acid solubilized in phosphate buffered saline (PBS) by paralimbal intrascieral injection. For comparison the same procedure was performed in the other 6 animals using PBS only. Following implantation the eyes were photographed and the area of neovascularisation measured after predetermined intervals. The results obtained 5 and 7 days post implantion are presented in tables 1 and 2. Table 1 Effect of a Single (Day 0) Intrascieral Injection of (2S)-2-(2,2- dimethylpropyloxycarboxamido)-3-{3,4-dihydro-2-[N-(2-imidazolyl)- carbamoylethyl]-(2S)-2H-1 ,4-benzoxazin-3-on-6-yl}propionic acid on bFGF- Stimulated Corneal Angiogenesis, 5 Day Post-Implantation 5 days post implantation neovascularisation was inhibited by 56.5 % (p < .01) in the group of animals receiving drug solution compared to the animal group receiving PBS only. Table 2 Effect of a Single (Day 0) Intrascieral Injection of (2S)-2-(2,2- dimethylpropyloxycarboxamido)-3-{3,4-dihydro-2-[N-(2-imidazoIyl)- carbamoylethyl]-(2S)-2H-1 ,4-benzoxazin-3-on-6-yl}propionic acid on bFGF- Stimulated Corneal Angiogenesis, 7 Day Post-Implantation 7 days post implantation neovascularisation was inhibited by 52.3 % (p < .01) in the group of animals receiving drug solution compared to the animal group receiving PBS only. The results obtained clearly demonstrate the advantagous effect of the present invention. Although only a single dosis of α v β 3 and/or α v β 5 inhibitor was given and drug formulation was only a solution, strong inhibition of neoascularization was performed over many days.

Claims: What claimed is: 1. Use of an v β 3 and/or α v βs inhibitor for the preparation of a medicament for prophylaxis and/or treatment of diseases of the eye of a patient resulting from angiogenesis in the eye, wherein the medicament is injected into the scleral layer of the eye of said patient through the location of the exterior surface of the sclera that overlies retinal tissue 2. Use according to Claim 1 wherein the α v β 3 and/or α v βs inhibitor is a RGD- containing polypeptide 3. Use according to Claim 2 wherein said polypeptide is a compound of formula I cyclo-(Arg-Gly-Asp-D-(A) n E) I, in which D is D-Phe, Phe, D-Trp, Trp, D-Tyr, Tyr, D-homoPhe, homoPhe, D-Nal, Nal, D-Phg, Phg or 4-Hal-Phe (D or L form), E is Val, Gly, Ala, Leu, lie or Nle and A is alkyl having 1-18 carbon atoms, n O or 1 and also their physiologically acceptable salts 4. Use according to Claim 2 wherein said polypeptide is a compound as expressed by subformula la, which otherwise correspond to formula I but in which D is D-Phe and E is Gly, Ala, Val, Leu, lie or Nle. 5. Use according to Claim 2 wherein said polypeptide is cyclo-(Arg-Gly-Asp-DPhe- Val) 6. Use according to Claim 2 wherein said polypeptide is cyclo-(Arg-Gly-Asp-DPhe- NMeVal) 7. Use according to Claim 2 wherein said therapeutically efective amount is from about 0.5 μg to 5 mg 8. Use according to Claim 2 wherein said eye disease is diabetic retinopathy 9. Use according to Claim 2 wherein said eye disease is macular degeneration 10. Use according to Claim 2 wherein said eye disease is myopia 11. Use according to Claim 2 wherein said eye disease is ocular histoplasmosis 12. Use according to Claim 1 wherein the v β 3 and/or α v βs inhibitor is a compound of formula II R»_W- wherein R 1 is H, alkyl having 1-6 C atoms or benzyl, R 2 is R 10 , CO-R 10 , COOR 6 , COOR 10 , SO 2 R 6 or SO 2 R 10 , R 3 is H, Hal, OA, NHR 10 , N(R 10 ) 2 , -NH-acyl, -O-acyl, CN, NO 2 , OR 10 , SR 10 , R 2 or CONHR 10 , R 4 is H, =O, =S, Cι-C 6 -alkyl or acyl, R 5 is NH 2 , H 2 N-C(=NH) or H 2 N-(C=NH)-NH, where the primary amino groups can also be provided with conventional amino protective groups or can be mono-, di- or trisubstituted by R 10 , CO-R 10 , COOR 10 or SO 2 R 10 ,or R 6 , R 7 , R 8 are each independently of one another absent or H, R 7 and R 8 together are also a bond, X, Y are each independently of one another =N-, -N-, O, S, -CH 2 - or =C-, with the proviso that at least one of the two definitions X, Y is =N-, -N-, O or S, W, Z are each independently of one another absent, O, S, NR 1 , C(=O), CONH, NHCO, C(=S)NH, NHC(=S), C(=S), SO 2 NH, NHSO 2 or CA=CA\ R 6 is a mono- or binuclear heterocycle which has 1 to 4 N, O and/or S atoms and can be unsubstituted or mono-, di- or trisubstituted by Hal, A, -CO-A, OH, CN, COOH, COOA, CONH 2 , NO 2 , =NH or =O, R 9 is H, Hal, OA, NHA, NAA', NHacyl, Oacyl, CN, NO 2 , SA, SOA, SO 2 A, SO 2 Ar or SO 3 H, R 10 is H, A, Ar or aralkyl having 7-14 C atoms, R 11 is H or alkyl having 1-6 C atoms, A, A' are each independently of one another H or unsubstituted or mono-, di- or tri-R 9 -substituted alkyl or cycloalkyl, each of which has 1-15 C atoms and in which one, two or three methylene groups can be replaced by N, O and/or S, Ar is unsubstituted or mono-, di- or tri-A- and/or R 9 -substituted mono- or binuclear aromatic ring system having 0, 1 , 2, 3 or 4 N, O and/or S atoms, Hal is F, Cl, Br or I and m, n are each independently of one another 0, 1 , 2, 3 or 4, or a the physiologically acceptable salts thereof 13. Use according to Claim 12 wherein the α v β and/or α v βs inhibitor is selected from the group consisting of compounds of subformulae Ila to llg, which otherwise correspond to formula II but in which in Ila) R 1 is H or alkyl with 1-6 C atoms, R 2 is R 10 , CO-R 10 , COOR 10 or SO 2 R 10 , R 3 is H, R 4 is H or =O, R 5 is H 2 N-C(=NH) or H 2 N-C(=NH)-NH, W, Z are each independently of one another absent, C(=O), NH, CONH or NHCO, X is -NH-, O or -CH 2 -, Y is NH or O, R 0 is H, A or benzyl, R 11 is H, A is unsubstituted alkyl or cycloalkyl with 1-15 C atoms and m, n are each independently of one another 0, 1 or 2; in lib) R 1 is H or alkyl with 1-6 C atoms, R 2 is R 10 , CO-R 10 , COOR 10 or SO 2 R 10 , R 3 is H, R 4 is H or =O, R 5 is R δ , W, Z are each independently of one another absent, C(=O), NH, CONH or NHCO, X is -NH-, O or -CH 2 -, Y is NH or O, R 6 is a mono- or binuclear heterocycle which has 1-4 N, O and/or S atoms and which can be unsubstituted or mono-, di- or trisubstituted by Hal, A, -CO-A, OH, CN, COOH, COOA, CONH 2 , NO 2 , =NH or =O, R 10 is H, A or benzyl, R 11 is H, A is unsubstituted alkyl or cycloalkyl with 1-15 C atoms and m, n are each independently of one another 0, 1 or 2; in lie) R 1 is H or alkyl with 1-6 C atoms, R 2 is R 10 , CO-R 10 , COOR 10 or SO 2 R 10 , R 3 is H, R 4 is H or =O, R 5 is H 2 N-C(=NH) or H 2 N-C(=NH)-NH, W, Z are each independently of one another absent, C(=O), NH, CONH or NHCO, X is -NH-, O or -CH 2 -, Y is NH or O, A is alkyl with 1-6 C atoms, R 10 is H, alkyl with 1-6 C atoms, camphor-10-yl or benzyl, R 11 is H, m, n are each independently of one another 0, 1 or 2; in lid) R 1 is H or alkyl with 1-6 C atoms, R 2 is R 10 , CO-R 10 , COOR 10 or SO 2 R 10 , R 3 is H, R 4 is H or =O, R 5 is R 6 , W, Z are each independently of one another absent, C(=O), NH, CONH or NHCO, X is =NH-, O or -CH 2 -, Y is H or O, R 6 is a mono- or binuclear heterocycle which has 1-4 N, O and/or S atoms and which can be unsubstituted or mono-, di- or trisubstituted by Hal, A, -CO-A, OH, CN, COOH, COOA, CONH 2 , NO 2 , =NH or =O, R 10 is H, alkyl with 1-4 C atoms, camphor-10-yl or benzyl, R 1 is H, A is unsubstituted alkyl with 1-6 C atoms and m, n are each independently of one another 0, 1 or 2; in lie) R 1 is H or alkyl with 1-6 C atoms, R 2 is R 10 , CO-R 10 , COOR 10 or SO 2 R 10 R 3 is H, R 4 is H or =O, R 5 is R 6 , W, Z are each independently of one another absent, C(=O), NH, CONH or NHCO, X is -NH-, O or -CH 2 -, Y is NH or O, R 6 is 1 H-imidazol-2-yl, thiazol-2-yl, 1 H-benzimidazol-2- yl, 2H-pyrazol-2-yl, 1 H-tetrazol-5-yl, 2-imino- imidazolidin-4-on-5-yl, 1-A-1 ,5-dihydro-imidazol-4- on-2-yl, pyrimidin-2-yl or 1 ,4,5,6-tetrahydro- pyrimidin-2-yl, R 10 is H, alkyl with 1-4 C atoms, camphor-10-yl or benzyl, R 1 is H, A is unsubstituted alkyl with 1-6 C atoms and m, n are each independently of one another 0, 1 or 2; in I If) R 1 is H or alkyl with 1-6 C atoms, R 2 is R 10 , CO-R 10 , COOR 10 or SO 2 R 10 , R 3 is H, R 4 is H or =O, R 5 is H 2 N-C(=NH) or H 2 N-C(=NH)-NH, W, Z are each independently of one another absent, C(=O), NH, CONH or NHCO, X is -NH-, O or -CH 2 -, Y is NH or O, R 10 is Ar, R 11 ' is H, A is unsubstituted alkyl or cycloalkyl with 1-15 C atoms and m, n are each independently of one another 0, 1 or 2; in llg) R 1 is H or alkyl with 1-6 C atoms, R 2 is R 10 , CO-R 10 , COOR 10 or SO 2 R 10 , R 3 is H, R 4 is H or =O, R 5 is R 6 , W, Z are each independently of one another absent, C(=O), NH, CONH or NHCO, X is -NH-, O or -CH 2 -, Y is NH or O, R 6 is a mono- or binuclear heterocycle which has 1-4 N, O and/or S atoms and which can be unsubstituted or mono-, di- or trisubstituted by Hal, A, -CO-A, OH, CN, COOH, COOA, CONH 2 , NO 2 , =NH or =O, R 10 is Ar, R 11 is H, A is unsubstituted alkyl or cycloalkyl with 1-15 C. atoms and m, n are each independently of one another 0, 1 or 2 14. Use according to Claim 12 wherein the α v β 3 and/or α v βs inhibitor is a compound selected from the group consisting of (2S)-2-[(R)-camphor-10-sulfonamido]-3-{3,4-dihydro-2-(3-guanidino- propyl)-(2R)-2H-1 ,4-benzoxazin-3-on-6-yl}propionic acid; (2S)-2-benzyloxycarboxamido-3-(2-guanidinomethyl-1 ,4-benzodioxan-6- yl)propionic acid; (2S)-2-tert-butyloxycarboxamido-3-[3,4-dihydro-2-(2-guanidino-2-oxoethyl)- 2H-1 ,4-benzoxazin-3-on-6-yl]propionic acid; (2S)-2-benzyloxycarboxamido-3-(2-guanidinoacet-amidomethyl-1 ,4-benzo- dioxan-6-yl)propionic acid; (2S)-2-tert-butyloxycarboxamido-3-{3,4-dihydro-2-[N-(2-imidazolyl)- carbamoylmethyl]-2H-1 ,4-benzox-azin-3-on-6-yl)propionic acid; (2S)-2-tert-butyloxycarboxamido-3-{3,4-dihydro-2-[N-(2-benzimidazolyl)- carbamoylmethyl]-2H-1 ,4-benzoxazin-3-on-6-yl)propionic acid; (2S)-2-tert-butyloxycarboxamido-3-{3,4-dihydro-2-[2-(2-imino-4- oxoimidazolidin-5-yl)ethyI]-2H-1 ,4-benzoxazin-3-on-6-yl}propionic acid; (2S)-2-(2,2-dimethylpropyloxycarboxamido)-3-{3,4-dihydro-2-[N-(2- imidazolyl)carbamoyIethyl]-(2S)-2H-1 ,4-benzoxazin-3-on-6-yl}propionic acid; (2S)-2-[(R)-camphorsulfonamido]-3-{3,4-dihydro-2-[N-(2-benzimidazolyI)- carbamoylmethyl]-2H-1 ,4-benzoxazin-3-on-6-yl)propionic acid and their physiologically acceptable salts 15. Use according to Claim 12 wherein the α v β 3 and/or α v βs inhibitor is (2S)-2-(2,2-dimethylpropyloxycarboxamido)-3-{3,4-dihydro-2-[N-(2- imidazolyl)carbamoylethyl]-(2S)-2H-1 ,4-benzoxazin-3-on-6-yl}propionic acid or (2S)-2-[(R)-camphorsulfonamido]-3-{3,4-dihydro-2-[N-(2-benzimidazolyl)- carbamoylmethyl]-2H-1 ,4-benzoxazin-3-on-6-yl)propionic acid 16. Use according to Claim 12 wherein said amount is from about 0.5 μg to 5 mg 17. Use according to Claim 12 wherein said eye disease is diabetic retinopathy 18. Use according to Claim 12 wherein said eye disease is macular degeneration 19. Use according to Claim 12 wherein said eye disease is myopia 20. Use according to Claim 12 wherein said eye disease is ocular histoplasmosis 21. Use according to Claim 1 wherein the α v β 3 and/or α v βs inhibitor is a compound of formula III in which R η is CH 2 OR 10 , COOR 10 , CONHR 10 or CON(R 12 ) 2) R 2 is R 10 , CO-R 10 , CO-R 6 , COOR 6 , COOR 10 , SO 2 R 6 , SO 2 R 10 , CONHR 6 , CON(R 6 ) 2 , CONHR 10 or CON(R 12 ) 2 , R is H, Hal, NHR 10 , N(R 12 ) 2 , NH-acyl, -O-acyl, CN, NO 2 , OR 10 , SR 10 , SO 2 R 10 , SO 3 R 10 , COOR 10 , CONHR 6 , CON(R 6 ) 2 , CONHR 10 or CON(R 12 ) 2 , R 4 is H, A, Ar or aralkylene having 7-14 C atoms, R 5 is NH 2 , H 2 N-C(=NH) or H 2 N-(C=NH)-NH, where the primary amino groups can also be provided with conventional amino protective groups, or can be mono- di- or trisubstituted by R 10 , CO-R 10 , COOR 10 or SO 2 R 10 , or R 6 -NH-, R° is a mono- or binuclear heterocycle having 1 to 4 N, O and/or S atoms, which can be unsubstituted or mono-, di- or trisubstituted by Hal, A, -CO-A, OH, CN, COOH, COOA, CONH 2 , NO 2 , =NH or =O, R 7 , R 8 in each case independently of one another is absent or is H, R 7 and R ε together are also a bond, Z is absent, O, S, NH, NR 1 , C(=O), CONH, NHCO, C(=S)NH, NHC(=S), C(=S), SO 2 NH, NHSO 2 or CA=CA', is H, Hal, OR 11 , NH 2 , NHR 12 , N(R 12 ) 2 , NHAcyl, OAcyl, CN, NO 2 , SR 11 , SOR 12 , SO 2 R 12 or SO 3 H, R 10 is H, A, Ar or aralkylene having 7-14 C atoms, R 11 is H or alkyl with 1-6 C atoms, R 12 is alkyl having 1-6 C atoms, A is H or alkyl having 1-15 C atoms or cycloalkyl having 3-15 C atoms, which is unsubstituted or is mono-, di- or trisubstituted by R 9 and in which one, two or three methylene groups can also be replaced by N, O and/or S, Ar is a mono- or binuclear aromatic ring system having 0, 1 , 2, 3 or 4 N, O and/or S atoms, which is unsubstituted or mono-, di- or trisubstituted by A and/or R 9 , Hal is F, Cl, Br or l, m, n in each case independently of one another are 0, 1 , 2, 3 or 4, and their physiologically acceptable salts and solvates 22. Use according to Claim 21 wherein the α v β 3 and/or α v βs inhibitor is selected from the group consisting of compounds of subformulae Ilia to llln, which otherwise correspond to formula III but in which in Ilia) R 3 is H; in lllb) R 3 is H and R 2 is COOR 10 or SO 2 R 10 ; in Illc) R 3 is H, R 2 is COOR 10 or SO 2 R 10 and R 10 is H, A, Ar or aralkylene having 7-14 C atoms; in Hid) m is O; in Hie) m is 0 and R 3 is H; in 11 If) R 3 is H, R 2 is COOR 10 or SO 2 R 10 and m is O; in lllg) R° is H, R 2 is COOR 10 or SO 2 R 10 and R 10 is H, A, Ar or aralkylene with 7-14 C atoms and m is O; in lllh) R 3 is H, R 2 is COOR 10 or SO 2 R 10 and R 10 is H, A, Ar or aralkylene having 7-14 C atoms and A is H or unsubstituted alkyl having 1-15 C atoms or cycloalkyl having 3-15 C atoms, Ar is phenyl or naphthyl and m is O; in llli) R b is a mono- or binuclear heterocycle having 1 to 4 N atoms, which can be unsubstituted or mono-, di- or trisubstituted by Hal, A, -CO-A, OH, CN, COOH, COOA, CONH 2 , NO 2 , =NH or =O; in R 3 is H, R 2 is COOR 10 or SO 2 R 10 and R 10 is H, A, Ar or aralkylene having 7-14 C atoms and m is O; R 6 is a mono- or binuclear heterocycle having 1 to 4 N atoms, which can be unsubstituted or mono-, di- or trisubstituted by Hal, A, -CO-A, OH, CN, COOH, COOA, CONH 2 , NO 2 , =NH or =O; in lllk) Z is absent; in llll) Z is absent and R 3 is H; in Him) Z is absent, R 3 is H and R 2 is COOR 10 or SO 2 R 10 ; in llln) Z is absent, R 3 is H, R 4 is H, R 2 is COOR 10 or SO 2 R 10 ; R 10 is H, A, Ar or aralkylene having 7-14 C atoms, R 6 is a mono- or binuclear heterocycle having 1 to 4 N atoms, which can be unsubstituted or mono-, di- or trisubstituted by Hal, A, -CO-A, OH, CN, COOH, COOA, CONH 2 , NO 2 , =NH or =O, A is H or unsubstituted alkyl having 1-6 C atoms, Ar is phenyl or naphthyl and m is 0 23. Use according to Claim 21 wherein the α v β3 and/or α v βs inhibitor is a compound selected from the group consisting of (2S)-3-[2-(3-aminopropyl)-4-oxo-4/-/-chromen-6-yl]-2-(2,2-dimethyIpropoxy- carboxamido)-propionic acid; (2S)-3-{2-[3-(1 H-imidazol-2-ylamino)propyl]-4-oxo-4H-chromen-6-yl}-2-(2,2- dimethylpropoxycarboxamido)propionic acid; (2S)-3-{2-[3-(1 H-imidazol-2-ylamino)propyl]-4-oxochroman-6-yl}-2-(2,2- dimethylpropoxycarboxamido)propionic acid; (2S)-3-{2-[3-(pyridin-2-ylamino)propyl]-4-oxo-4H-chromen-6-yl}-2-(2,2- dimethylpropoxycarboxamido)propionic acid; (2S)-3-{2-[3-(1 H-benzimidazol-2-ylamino)propyl]-4-oxo-4H-chromen-6-yl}-2- (2,2-dimethylpropoxycarboxamido)propionic acid; (2S)-3-{2-[3-(1 H-imidazol-2-ylamino)propyl]-4-oxo-4 -/-chromen-6-yl}-2- butylsulfonamidoprόpionic acid (2S)-3-{2-[3-(pyridin-2-ylamino)propyl]-4-oxo-4H-chromen-6-yl}-2-(2,4,6- trimethylphenyl)sulfonamidopropionic acid and their physiologically acceptable salts and solvates 24. Use according to Claim 21 wherein the α v β3 and/or α v βs inhibitor is a compound selected from the group consisting of (2S)-3-{2-[3-(1 H-imidazol-2-ylamino)propyl]-4-oxo-4AV-chromen-6-yl}-2- butylsulfonamidopropionic acid and (2S)-3-{2-[3-(pyridin-2-ylamino)propyl]-4-oxo-4H-chromen-6-yl}-2-(2,4,6- trimethylphenyl)sulfonamidopropionic acid 25. Use according to Claim 21 wherein said amount is from about 0.5 μg to 5 mg 26. Use according to Claim 21 wherein said eye disease is diabetic retinopathy 27. Use according to Claim 21 wherein said eye disease is macular degeneration 28. Use according to Claim 21 wherein said eye disease is myopia 29. Use according to Claim 21 wherein said eye disease is ocular histoplasmosis 30. Use according to Claim 1 wherein the α v β 3 and/or α v βs inhibitor is a compound of formula IV wherein A and B are each independently of one another O, S, NH, NR 7 , CO, CONH, NHCO or directly bond, X is alkylene having 1-2 C atoms, which is unsubstituted or monosubstituted by R 4 or R 5 or a direct bond, R 1 is H, Z or -(CH 2 ) 0 -Ar, R 2 is H, R 7 or -C(O)Z, R 3 is NHR 6 , -NR 6 -C(=NR 6 )-NHR 6 , -C(=NR 6 )-NHR 6 , -NR 6 -C(=NR 9 )- NHR 6 , -C(=NR 9 )-NHR 6 or Het 1 , R 4 or R 5 are each indipendently of one another H, oxo, R 7 , -(CH 2 ) 0 -Ar, -C(O)-(CH 2 ) 0 -Ar, -C(O)-(CH 2 ) 0 -R 7 , -C(O)-(CH 2 ) 0 -Het, Het, NHR 6 , NHAr, NH-Het, OR 7 , OAr, OR 6 or O-Het, R 6 is H, -C(O)R 7 , -C(O)-Ar, R 7 , COOR 7 , COO-(CH 2 ) 0 -Ar, SO 2 -Ar, SO 2 R 7 or SO 2 -Het, R 7 is alkyl having 1 to 10 C atoms or cycloalkyl having 1 to 10 C atoms, R 8 is Hal, NO 2 , CN, Z, -(CH 2 ) 0 -Ar, COOR 1 , OR 1 , CF 3 , OCF 3 , SO 2 R 1 , NHR 1 , N(R 1 ) 2 , NH-C(O)R 1 , NHCOOR 1 or C(O)R 1 , R 9 is CN or NO 2 , Z is alkyl having 1 to 6 C atoms, Ar is aryl, which is unsubstituted or substituted by R 8, Hal is F, Cl, Br or l, Het is unsaturated, partly of fully saturated mono- or bicyclic heterocyclic ring system having 5 to 10 atoms, which can contain 1 or 2 N atoms and/or 1 or 2 S or O atoms and wherein the heterocyclic ring system can be mono or disubstituted by R 8 , Het 1 is a mono or bicyclic aromatic heterocyclic ring system having 1 to 4 N atoms, which can be unsubstituted or mono or disubstituted by Hal, R 7 , OR 7 , CN, NHZ or NO 2 , n is 0, 1 or 2 m is 0, 1 , 2, 3, 4, 5 or 6, o is O, 1 or 2 as well as their physiologically acceptable salts and solvates 1. Use according to Claim 30 wherein the α v β3 and/or α v βs inhibitor is selected of the group consisting of compounds of subformulae IVa to IVi, which otherwise correspond to formula IV but in which in IVa X is a direct bond inlVb X is a direct bond, R 2 isH, R 5 is H and R 4 isAr inlVc X is a direct bond, R 5 is H and R 4 is Ar or Het; inlVd X is a direct bond, R 5 isH, B isO, A isNH, n isO, m is 3 or 4, R 3 is Het and R 4 is Ar inlVe X is a direct bond, R 5 isH, B isO, A isNH, n isO, m is 3 or 4 and R 3 is Het inlVf X is methylene, which is unsubstituted or substituted byAr, R^ isH, R 5 is H oder Ar and R 4 is oxo inlVg X is methylene, R 3 -(CH 2 ) n -A-(CH 2 ) m -B IVg inlVh X is methylene, R 4 is H or Ar, R 5 is H orArand R 2 isH; in IVi X is methylene, R 4 is H or Ar, R 5 is H orAr, B isO, A isNH, n isO, m is 3 or 4 R 3 is Het and R 2 isH 32. Use according to Claim 30 wherein the α v β 3 and/or α v βs inhibitor is a compound selected from the group consisting of 3-phenyl-3-{6-[3-(pyridine-2-ylamino)-propoxy]-1 H-indole-3-yl}-propionic acid; 3-phenyl-3-{6-[4-(pyridine-2-ylamino)-butoxy]-1 H-indole-3-yl}-propionic acid; 3-phenyl-3-{5-[4-(pyridine-2-ylamino)-butoxy]-1 H-indole-3-yl}-propionic acid; 3-phenyl-3-{5-[3-(pyridine-2-ylamino)-propoxy]-1 H-indole-3-yl}-propionic acid; 3-phenyl-3-[6-(pyridine-2-yl-amidocarboxymethoxy)-indole-3-yl]-propionic acid; 3-phenyl-3-[6-(benzimidazole-2-yl-amidocarboxymethoxy)-indole-3-yI]- propionic acid or 3-phenyl-3-[6-(imidazole-2-yl-amidocarboxymethoxy)-indole-3-yl]-propionic acid as well as their physiologically acceptable salts and solvates 33. Use according to Claim 30 werein wherein the α v β 3 and/or α v βs inhibitor is 3-phenyl-3-{6-[3-(pyridine-2-ylamino)-propoxy]-1 H-indole-3-yl}-propionic acid 34. Use according to Claim 30 wherein said amount is from about 0.5 μg to 5 mg 35. Use according to Claim 30 wherein said eye disease is diabetic retinopathy 36. Use according to Claim 30 wherein said eye disease is macular degeneration 37. Use according to Claim 30 wherein said eye disease is myopia 38. Use according to Claim 30 wherein said eye disease is ocular histoplasmosis 39. Use of an α v β 3 and/or α v βs inhibitor for the preparation of a medicament for prophylaxis and/or treatment of diseases of the eye of a patient resulting from angiogenesis in the eye, wherein the medicament comprise nanoparticles containing a therapeutically effective amount of an α v β3 and/or α v β 5 inhibitor sufficient to inhibit angiogenesis and is injected into the scleral layer of the eye of said patient through the location of the exterior surface of the sclera that overlies retinal tissue 40. Use according to Claim 39 characterized in that the nanoparticles contain a biocompatible polymer 41. Use according to Claim 39 characterized in that the nanoparticles contain a biodegradable polymer 42. Use according to 41 characterized in that the polymer is poly(lactic acid) (PLA), poly(glycolic acid) (PGA), polycaprolactone (PCL), a copolymer of lactic acid and glycolic acid (PLGA), a copolymer of lactic acid and caprolactone, polyepsilon caprolactone, polyhyroxy butyric acid, a poly(ortho)ester, a polyurethane, a polyan hydride, a polyacetal, a polydihydropyran or a polycyanoacrylate 43. Use according to Claim 39 characterized in that the composition comprise a liquid medium wherein the nanoparticles are being dispersed thereby forming a colloidal suspension 44. Use according to Claim 39, characterized in that the nanoparticles have a diameter from about 10 nm to about 500 nm 45. Use according to Claim 39 characterized in that the nanoparticles have a diameter from about 100 nm to about 200 nm 46. Use according to Claim 39 characterized in that the nanoparticles have been prepared by solvent displacement process

Inventors: BENDER HANS-MARKUS, FRIEDLANDER MARTIN, HAUNSCHILD JUTTA, LANG ULRICH, WIESNER MATTHIAS, BENDER, HANS-MARKUS, FRIEDLANDER, MARTIN, HAUNSCHILD, JUTTA, LANG, ULRICH, WIESNER, MATTHIAS
Applicants: BENDER HANS MARKUS, FRIEDLANDER MARTIN, HAUNSCHILD JUTTA, LANG ULRICH, MERCK PATENT GMBH, WIESNER MATTHIAS, BENDER, HANS-MARKUS, FRIEDLANDER, MARTIN, HAUNSCHILD, JUTTA, LANG, ULRICH, WIESNER, MATTHIAS
Application year: 2001
Main classification: A61K38
IPC codes: A61K9, A61K31, A61K38, A61K45, A61K47, A61P3, A61P9, A61P27, A61P31, A61P43, C07D311, C07D401, C07D403, C07D405, C07D413