INHIBITORY PAS DOMAIN PROTEIN (IPAS) AND SCREENING METHODS RELATED TO ANGIOGENESIS AND TUMOR PROGRESSION

Abstract: The present invention relates a mammalian polypeptide designated Inhibitory PAS Domain Protein (IPAS) which polypeptide is useful for the inhibition of angiogenesis and/or tumor progression. The invention also relates to screening methods for compounds potentially useful as medicaments for the treatment of medical conditions related to angiogenesis or tumor progression.

Description: Inhibitory PAS domain protein (IPAS) and screening methods related to angiogenesis and tumor progression TECHNICAL FIELD The present invention relates a mammalian polypeptide designated Inhibitory PAS Domain Protein (IPAS) which polypeptide is useful for the inhibition of angiogenesis and/or tumor progression. The invention also relates to screening methods for compounds potentially useful as medicaments for the treatment of medical conditions related to angiogenesis or tumor progression. BACKGROUND ART Oxygen plays a critical biological role as the terminal electron acceptor in the mitochondria of vertebrate cells. During evolution, these cells have developed ways to sense alterations in oxygen levels and, during this process, acquired the ability to conditionally modulate the expression of genes involved in adaptive physiological responses to hypoxia including angiogenesis, erythropoiesis, and glycolysis. These genes include vascular endothelial growth factor, eryhtropoietin, several glycolytic enzymes and inducible nitric oxide synthase, and have all been shown to contain hypoxia responsive elements (HREs) (for reviews, see Guillemin and Krasnow (1997) Cell 89, 9-12; Wenger and Gassmann (1997) Biol. Chem. 378, 609-616). Under hypoxic conditions these response elements are recognized by a heterodimeric complex consisting of the hypoxia inducible factor- lα (EQF- l ) and Arnt (Wang et al. (1995) Proc. Natl. Acad. Sci. USA 92, 5510-5514; Gradin et al. (1996) Mol. Cell. Biol. 16, 5221-5231). Both these transcription factors belong to the rapidly growing family of basic-helix-loop-helix (bHLH)-PAS (Per, Arnt, Sim) proteins. A family of helix-loop-helix proteins designated Id 18 has been identified as antagonists of bHLH transcriptional regulators. bHLH proteins typically bind regulatory sequences in a heterodimeric configuration and function to activate differentiation-linked gene expression. The heterodimer usually comprises a class A bHLH protein together with a class B bHLH protein. In the presence of excess Id protein, the class A bHLH partner is typically titrated out through heterodimerization with Id protein. Dysregulation or overfunction of HIF-l might cause a variety of pathological conditions including tumor progression 15 16 7 and inflammatory angiogenesis 17 . Consequently, there is a need for identification of compounds acting as negative regulators of HIF-lα, said compounds being potentially useful against medical conditions related to angiogenesis and tumor progression. DESCRIPTION OF THE DRAWINGS Fig. 1: Mouse IPAS sequence and expression (a) Deduced amino acid sequence of mouse IPAS. Domains of basic helix-loop-helix and PAS A and B are shown. (b) Schematic representation of structural features of IPAS and the hypoxia-inducible factors. The percent identities of each protein with IPAS within the bHLH and PAS motifs are shown. bHLH, basic helix-loop-helix; PAS, Per/Arnt/Sim; N- or C-TAD, N- or C-terminal transactivation domain. (C) Northern blot analysis of IPAS expression in adult mouse tissues. Poly(A) + RNA(4.5 μg) from various adult mouse tissues were hybridized with 32 P-labeled IPAS cDNA probe. The positions of RNA markers are shown on the right in kb. (d)-(o) In situ hybridization analysis. Sections of cornea (d-g), retina (h-k), and cerebellum (l-o) of adult mouse were hybridized with antisense RNA porbes of mlPAS (d, e, h, i, 1, and m) or mHTF-lα (f, g, j, k, n, and o). Light- (d, f, h, j, 1, and n) and dark-field (e, g, i, k, m, and o) views are shown. C, cornea; Ep, epithelium; S, substantia propria; LE, lens epithelium; GC, granular cell layer; INL, inner nuclear layer; R&C, rods and cones, G, granular layer; P, Purkinje cells; M, molecular layer. Fig. 2: PAS is a dominant negative regulator of hypoxia-inducible factors (a) IPAS does not transactivate HRE-driven reporter gene. Increasing amounts of IPAS expression vector (CMV IPAS) were cotransfected with HRE-luciferase reporter gene into HeLa cells. The cells were cultured under either normoxic (21% O 2 ) or hypoxic (1% O 2 ) conditions for 24 h and the cellular luciferase expression was determined. (b), (c) IPAS inhibits hypoxia-inducible factors-mediated gene expression. IPAS expression vector, HRE-luciferase reporter, and HIF-lα (b) or HLF (c) expression vector (CMV HDF- lα or CMV HLF, respectively) were introduced into HeLa cells. After 24 h-incubation in normoxic (21% O 2 ) or hypoxic (1% O ) condition, luciferase activity was measured. Results were expressed as fold induction compared with the luciferase activity in the cells transfected with reporter gene alone. Means ± SD were shown. (d) IPAS does not affect on HIF-lα and HLF protein levels. HeLa cells were transfected with FLAG-tagged IPAS expression plasmid (1.0 μg/21.5 cm 2 dish) and exposed to hypoxia for 6 h. Whole cell extracts (50 μg) were prepared and subject to immunoblot analysis using antibodies against HIF-lα (Novus), HLF (Novus), and FLAG epitope (Sigma) essentially as previously described ". Fig. 3: IPAS specifically attenuates hypoxia-inducible RNAs expression (a) Hypoxia-inducible gene expression is impaired in IPAS overexpressing cells. Wild type(Hepalclc7) or IPAS-stably transfected (Hepa IPAS) mouse hepatoma cell lines were cultured in either normoxic (N) or hypoxic (H) conditions for 24h. Poly(A) + RNA from the cells were separated and hybridized with radiolabeled mouse IPAS, PGK1, VEGF, and β- actin cDNA probe as indicated. (b) Inhibition of hypoxia-inducible gene expression by IPAS at transcriptional level. HRE- luciferase reporter was transfected with or without HIF-lα expression vector into Hepalclc7 or Hepa IPAS cells. The cells were cultured under conditions of either 21 % or 1 % O concentration and subject to luciferase assay. Luciferase content of the Hepalclc7 cells transfected with the reporter gene alone was served as a control and results were shown as fold induction compared with the control. (c) IP AS inhibits the binding of HIF-lα/ Arnt complex to HRE. Nuclear extracts from normoxic or hypoxic Hepalclc7 and Hepa IPAS cells were analyzed by EMSA using 32 P- labeled HRE oligonucleotide probe. The asterisks shows position of constitutive HRE- binding activity in the nuclear extracts and arrow shows the position of hypoxia-induced HIF-l Arnt-DNA complex 12. Competition assay by unlabeled HRE (S) or unrelated sequence(NS) and supershift formation by anti HIF-lα antibody and anti-Arnt antibody were shown. (d) IP AS does not affect on dioxin-inducible gene expression. Hepalclc7 and Hepa IPAS cells were treated with or without TCDD (10 nM) for 24 h and poly(A) + RNA from the cells was hybridized with 32 P-labeled mouse CYP1A1, IPAS, and β-actin cDNA probe. (e) IPAS has no effect on TCDD-mediated XRE-reporter gene expression. Heρalclc7 and Hepa IPAS cells were transfected with XRE-reporter plasmid and after incubation with or with out TCDD for 24h luciferase activity was monitored. Results were expressed as fold induction of luciferase activity compared to the ligand free control of Hepalclc7 cells. Means ± SD were shown. Fig. 4: IPAS targets HIF-l to form a nonfunctional complex (a) IPAS physically interacts with Hff-l . In vitro-translated GST-IPAS or GST was mixed with 35 S-labeled, in vitro translated Arnt or HIF-lα and immunoprecipitation with anti- GST antibody was carried out. The precipitant was separated by SDS-PAGE followed by autoradiography. For the loading control, 10% of input Arnt and HIF-lα were shown. (b) The N-terminal structure of HIF-lα is essential for the heterodimerization with PAS. 35 S- labeled, in vitro translated IPAS was incubated with GAL4-fusion of various fragments of HIF-lα and subject to the immunoprecipitation with either anti GAL4 antibody or preimmune control serum. Precipitated fraction was analyzed by SDS PAGE and results were obtained by autoradiography. Ten percent of input IPAS was shown as a control. (c), (d) In vivo interaction between PAS and HP-lα. COS7 cells were transfected with various amounts of expression vectors for GAL4- HP-lα 1-330 and VP16-PAS (c) or GAL4- PAS and VP 16- Arnt (d) as indicated together with GAL4-driven reporter gene. After 24h- incubation, cellular luciferase activity was determined. Results were expressed as fold induction compared with the luciferase contents of the cells transfected with reporter gene alone. (e) PAS/HP- lα heterodimer fails to bind to HRE. Various combinations of in vitro translated PAS, HP-lα, and Arnt, or unprogrammed reticulocyte lysate as indicated were mixed with 32 P-labeled HRE oligonucleotide probe, and the protein-DNA complex formation was monitored by EMSA. Results were visualized by autoradiography. Fig. 5: Involvement of PAS in silencing the production of angiogenic growth factor in cornea epithelium cells Primary culture of mouse cornea epithelium cells were transfected with either antisense PAS expression plasmid or empty vector (vector) and incubated under normoxic (N, 21% O 2 ) or hypoxic (H, 1% O 2 ) conditions for 24 h. Total RNAs from the cells were extracted and Northern blot analysis using radiolabeled mouse VEGF cDNA probe was performed. Total RNAs form normoxic and hypoxic Hepalclc7 cells were supplied as a reference for VEGF induction. As a loading control, 18S RNA levels are shown. DISCLOSURE OF THE INVENTION The present invention provides a model wherein activated HP-lα encounters a negative regulation by a small protein factor such as PAS, to form a nonfunctional heterodimeric complex. This mode of regulation of HP-lα might contribute to a fine-tuning of hypoxia signaling in situ as evidenced by profound negative effect of PAS in corneal VEGF production. On the other hand, ectopic expression of PAS potentially repressed hypoxia- inducible VEGF expression, and the negative effect of PAS was selective to hypoxia signaling so far tested. Therefore, it is postulated that PAS is useful as a target in therapeutic drug design for various angiogenic diseases, such as ischemic cardiovascular lesions, stroke, and diabetic microvascular diseases. Consequently, in a first aspect this invention provides an isolated nucleic acid molecule selected from: (a) nucleic acid molecules comprising a nucleotide sequence set forth as SEQ ID NO: 2; (b) nucleic acid molecujes comprising a nucleotide sequence capable of hybridizing, under stringent hybridization conditions, to a nucleotide sequence complementary the polypeptide coding region of a nucleic acid molecule as defined in (a) and which codes for a biologically active mammalian PAS polypeptide or a functionally equivalent modified form thereof; and (c) nucleic acid molecules comprising a nucleic acid sequence which is degenerate as ^ result of the genetic code to a nucleotide sequence as defined in (a) or (b) and which codes for a biologically active mammalian PAS polypeptide r a functionally equivalent modified form thereof. The nucleic acid molecules according to the present invention includes cDNA, chemically synthesized DNA, DNA isolated by PCR, genomic DNA, and combinations thereof, Genømic DNA may be obtained by screening a genomic library with the PAS cDNA described herein, using methods that are well known in the art. RNA transcribed from DNA is also encompassed by the present invention. The term "stringent hybridization conditions" is known in the art from standard protocols (e.g. Ausubel et al., supra) and could be understood as e.g. hybridization to filterr-bound DNA in 0.5 M NaHPO 4 , 7% sodium dodecyl sulfate (SDS), 1 mM EDTA at +65°C, and washing in 0. JxSSC / 0.1% SDS at +68°C. In a preferred form of the invention, the said nucleic acid molecule has a nucleotide sequence identical with SEQ P> NO: 2 of the Sequence Listing. However, the nucleic acid molecule according to the invention is not to be limited strictly to the sequence shown as SEQ ID NO: 2. Rather the invention encompasses nucleic acid molecules carrying modifications like substitutions, small deletions, insertions or inversions, which nevertheless encode proteins having substantially the biochemical activity of the PAS polypeptide according to the invention. Included in the invention are consequently nucleic acid molecules, the nucleotide sequence of which is at least 90% homologous, preferably at least 95% homologous, with the nucleotide sequence shown as SEQ ID NO: 2 in the Sequence Listing. Included in the invention is also a nucleic acid molecule which nucleotide sequence is degenerate, because of the genetic code, to the nucleotide sequence shown as SEQ ID NO: 2. A sequential grouping of three nucleotides, a "codon", codes for one amino acid. Since there are 64 possible codons, but only 20 natural amino acids, most amino acids are coded for by more than one codon. This natural "degeneracy", or "redundancy", of the genetic code is well known in the art. It will thus be appreciated that the nucleotide sequence shown in the Sequence Listing is only an example within a large but definite group of sequences which will encode the PAS polypeptide. In a further aspect, this invention provides an isolated mammalian PAS polypeptide encoded by the nucleic acid molecule as defined above. In a preferred form, the said polypeptide has an amino acid sequence according to SEQ ID NO: 3 of the Sequence Listing. However, the polypeptide according to the invention is not to be limited strictly to a polypeptide with an amino acid sequence identical with SEQ ID NO: 3 in the Sequence Listing. Rather the invention encompasses polypeptides carrying modifications like substitutions, small deletions, insertions or inversions, which polypeptides nevertheless have substantially the biological activities of the PAS polypeptide. Included in the invention are consequently polypeptides, the amino acid sequence of which is at least 90% homologous, preferably at least 95% homologous, with the amino acid sequence shown as SEQ TO NO: 3 in the Sequence Listing. hi yet another aspect, the invention provides a vector comprising the nucleic acid sequence as defined above. The term "vector" refers to any carrier of exogenous DNA that is useful for transferring the DNA to a host cell for replication and/or appropriate expression of the exogenous DNA by the host cell. The said vector can be a replicable expression vector, which carries and is capable of mediating the expression of a nucleic acid sequence according to the invention. In the present context, the term "replicable" means that the vector is able to replicate in a given type of host cell into which it has been introduced. Examples of vectors are viruses such as bacteriophages, cosmids, plasmids and other recombination vectors. Nucleic acid molecules are inserted into vector genomes by methods well known iff the art. Included in the invention is also a cultured host cell harboring a vector accprding to the. invention. Such a host cell can be a prokaryotic cell, a unicellular eukaryotic cell or a cell derived from a multicellμlar organism. The host cell can thus e.g. be a bacterial cell such as an E, colt cell; a cell from a yeast such as Saccharomyces cervisiae or Pichiapastoris, or a mammalian cell. The methods employed to effect introduction of the vector into the host cell are standard methods well known to a person familiar with recombinant DNA methpds. The invention also includes a process for production of a mammalian PAS polypeptide, comprising culturing the. said host cell under conditions whereby said polypeptide is produced, and recovering said polypeptide. In a further important aspect, the invention provides a (screening) method for identifying an agent useful for activating the expression of a mammalian PAS nucleic acid molecule, said method comprising the steps (i) contacting a candidate agent with mammalian PAS nucleotide acid molecule, or with a rnarnmalian PAS polypeptide, according to the invention; and (ii) determining whether said candidate agent activates the expression of the said mammalian PAS nucleic acid molecule, or stimulates the biological activities of the said polypeptide. For screening purposes, appropriate host cells can be transformed with a vector having a reporter gene under the control of the PAS gene according to this invention. The expression of the reporter gene can be measured in the presence or absence of an agent with known activity (i.e. a standard agent) or putative activity (i.e. a "test agent" or "candidate agent"). A change in the level of expression of the reporter gene in the presence of the test agent is compared with that effected by the standard agent. In this way, active agents are identified and their relative potency in this assay determined. As used herein, the term "reporter gene" means a gene encoding a gene product that can be identified using simple, inexpensive methods or reagents and that can be operably linked to an PAS sequence. Reporter genes such as, for example, a luciferase, β-galactosidase, alkaline phosphatase, or green fluorescent protein reporter gene, can be used to determine transcriptional activity in screening assays according to the invention (see, for example, Goeddel (ed.), Methods Enzymol., Vol. 185, San Diego: Academic Press, Inc. (1990); see also Sambrook, supra). As used herein, the term "agent" means a biological or chemical compound such as a simple or complex organic molecule, a peptide, a protein or an oligonucleotide. Such an agent, identified in the methods according to the invention, is potentially useful e.g. in the identification, development and manufacture of medicaments for the inhibition of angiogenesis and/or tumor growth, including angiogenic diseases related to ischemic cardiovascular lesions, stroke, or diabetic microvascular diseases. Consequently, the invention also provides a method for the treatment of angiogenic disease or tumor growth, comprising administering to a subject an effective amount of an agent identified by the method described above. The term "treatment" means any treatment of a diseases in a mammal, including: (i) preventing the disease, i.e. causing the clinical symptoms of the disease not to develop; (ii) inhibiting the disease, i.e. arresting the development of clinical symptoms; and/or (iii) relieving the disease, i.e. causing the regression of clinical symptoms. The term "effective amount" means a dosage sufficient to provide treatment for the disease state being treated. This will vary depending on the patient, the disease and the treatment being effected. EXPERIMENTAL METHODS Throughout this description the terms "standard protocols" and "standard procedures", when used in the context of molecular biology techniques, are to be understood as protocols and procedures found in an ordinary laboratory manual such as: Current Protocols in Molecular Biology, editors F. Ausubel et al., John Wiley and Sons, Inc. 1994, or Sambrook, J., Fritsch, E.F. and Maniatis, T., Molecular Cloning: A laboratory manual, 2nd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY 1989. Plasmid Construction pcDNA3 PAS was made by insertion of the EeøRI-NotI fragment from pT7T3D PAS (GenBank Ace: AA028416) into EcøRI-Nøtl digested pcDΝA3 plasmid (Invitrogen). PCMV PAS or pFLAG PAS contained Hindm-Xbaϊ or BamHI-Xbal fragment from pcDNA3 PAS in corresponding site of pCMV4 or pCMV FLAG plasmid, respectively ° " . HRΕ-luciferase, XRΕ-luciferase, and pCMV HP-lα are described elsewhere 12 20 . pBluescript mHLF was gift from Dr. Y. Fujii-Kuriyama and used for construction of pCMV mHLF. For the construction of the pGST, a plasmid for in vitro translation of GST- fusion protein, GST cDNA and multiple cloning site of pGΕX-4T-3 (Amersham Pharmacia Biotech) was amplified by PCR with BglE and Hind E linker, and subcloned into BglTi.- HindΗl site of pSP72 vector (Promega). pGST PAS was made by insertion of PCR-cloned PAS cDNA with BamΑl and Xhol linker into BamHl-XhoI-digested pGST. GAL4 HP- lα/1-826, 1-330, 1-652, 526-826 were as previously described 6 . For pCMX GAL4-PAS or pCMX VP-16 PAS construction, EcoRl-Xbal or BamBI-Xbal fragment of pcDNA3 PAS was inserted into EcoRI-Nhel site of pCMX GAL4 or to BamΑI-Nheϊ site of pCMX VP16, respectively. PCMXVP16-Arnt was gift from Dr. I. Pongratz. To make antisense PAS expression plasmid, full length PAS cDNA with EcoRI-BamHl linker was inserted in inverted direction to i?αmHI-EcøRI site of pcDNA3 plasmid. Cell Culture and Transfection Hepalclc7, HeLa, and COS7 cells were from ATCC. Hepa PAS cells were established by stable transfection of Hepalclc7 cells with pΕFIRΕSpuro PAS and puromycin (5 μg/ml) selection. Transient transfections were carried out by the lipofection procedure in 28 cm 2 culture plates. In luciferase assay, 0.5 μg of reporter plasmids and indicated amounts expression plasmids were transfected. Hypoxic- or TCDD treatment of the cells was previously described 12 . Northern Blot and In Situ Hybridization Analysis Poly(A) + RNAs (4.5 μg) from various tissues of 8 week-old C57B16 mice or Hepalclc7 and Hepa PAS cells were obtained by guanidiumthiocyanate methods followed by oligo dT-beads purification (Dynal) and analyzed by Northern blot using 32 P-labeled cDNA probes of mPAS (nt 623-897), mPGKl (nt 426-771), mVEGF3 (nt 24-466) , mCYPlAl (nt 874-1199), and β-actin (nt 930-1075). Total RNA (20 μg) from mouse corneal epithelium primary culture or Hepalclc7 cells was separated and probed with radiolabeled mVEGF3 cDNA (nt 24-466) probe. In situ hybridization of tissue sections from 8 week-old C57B16 mice using 35 S -labeled mPAS or mHP-lα antisense RNA probe was performed as previously described 21 . Electrophoretic Mobility Shift Assay Nuclear extracts from either normoxic or hypoxic cells were prepared as described before 12 . Ten microgram of the nuclear extract was incubated with 32 P-labeled HRE oligonucleotide in a buffer containing 0.1 μg of sonicated, denatured calf thymus DNA in 10 mM Tris-HCl (pH 7.5), 60 mM KC1, 50 inM NaCl, 1 mM MgCl 2 , 1 mM EDTA, 5 mM dithiothreitol (DTT), 5% glycerol. Various combination of in vitro translated proteins (5 μl each) were mixed with HRE probe in a solution containing 10 mM Hepes, 100 mM KCL, 0,1 mM EDTA, 3 mM MgCl 2 , 4 mM spermidine, 0.5 mM DTT, 10 % glycerol, 20 ng/μl tRNA, 1 ng/μl salmon-sperm DNA. The protein-DNA complexes were separated on 4% polyacrylamid gel in 0.5X TBE buffer (lxTBE; 89 mM Tris, 89 mM Boric acid, 5 mM EDTA). In Vitro Protein Interaction Assay GST-fused PAS or GAL4-fusion of various fragments of HP-l were generated by translation either in the presence or absence of 35 S-labeled methionine in rabbit reticulocyte lysate (Promega). Protein concentration of GST-PAS or GAL4- HP- las was determined on the basis of incorporated 35 S-labeled methionine. Equal amount of 5 S-labeled, in vitro translated Arnt, HP-lα or PAS were incubated with GST PAS or GAL4- HP- las for 1 h at room temperature, followed by incubation with anti-GST antibody (Amersham Pharmacia Biotech) or anti-GAL4 antibody (Upstate Biotechnology) conjugated Protein A Sepharose (Amersham Pharmacia biotech) for another 1 h at room temperature. After brief centrifugation, coimmunoprecipitated proteins were analyzed by SDS-PAGE. Isolation ofMurine Corneal Epithelial Cells Six-week-old C57B16/J healthy mice were killed with a lethal dose of CO 2 . The eyes were enucleated and the corneal tissue was dissected in DME medium supplemented with 10% bovine calf serum under a stereomicroscope. The corneal tissue was cut into small pieces under sterile conditions and washed with DMEM twice. The tissue masses were placed onto a gelatin-coated tissue culture plate and incubated in DMEM with 10% bovine calf serum supplemented with human recombinant FGF-β at the concentration of 3 ng/ml. After incubation in 5% CO 2 for 8 days, corneal epithelial cells grown to nearly confluence were trypsinized. A single cell suspension was then seeded onto 21.5 cm 2 culture dishes and cells were grown under the same condition as described above. EXAMPLES EXAMPLE 1: Identification of PAS sequence Hidden Markov Model profiles 3 were designed using the HMMER 1,8,3 software 19 from nucleotide sequences corresponding to the PAS domain of a selected number of bHLH/PAS factors. A mouse EST database at GenBank (http://www.ncbi.nlm.nih.gov) was screened and an EST clone of 460 bp (GenBank Ace: AA028416; SEQ ID NO: 1) containing a bHLH (basic-helix-loop-helix) PAS motif, was identified. DNA sequence analysis revealed that PAS cDNA (SEQ ID NO: 2) contains an open reading frame of 921 nucleotides, encoding a polypeptide of 307 amino acids (Fig. la; SEQ ID NO: 3). The predicted polypeptide was designated PAS (Inhibitory PAS Domain Protein) Alignment analysis of this amino acid sequence with known bHLH PAS factors showed high similarity to HP-lα 4 and HLF 5 in the N-terminal bHLH domain (75 % and 76 % identity, respectively; Fig lb), and to a lesser extent within PAS region (34% and 36 % in the PAS A, and 40 % and 36 % in the PAS B domain, respectively; Fig. lb). Notably, PAS lacks the sequence corresponding to C-terminal region of HP-lα and HLF, in which two transactivation domains (NT AD and CTAD) have been identified. EXAMPLE 2: PAS mRNA is expressed predominantly in the eye Northern blot analysis of poly(A) + RNA from a variety of mouse tissues demonstrated that PAS mRNA was expressed predominantly in the eye and at lower levels in the cerebellum and the cerebrum. No obvious expression in was detected in other tested mouse tissues, indicating a very tissue-restricted expression pattern of PAS mRNA (Fig. lc). EXAMPLE 3: PAS expression is observed in the epithelial cell layer of the cornea To characterize the spatial expression pattern of the PAS gene in the eye and cerebellum, in situ hybridization was performed. Intense PAS expression was observed in the epithelial cell layer of the cornea (Fig. Id and le) and with lower intensity in the layers of ganglion cells, inner nuclear cells, and rods and cones of the retina (Fig. lh and li). Expression of HP-lα mRNA was detected by in situ hybridization at low levels in the epithelium of the cornea (Fig. If and lg), demonstrating remarkably dominant expression of PAS over HP-lα in these cells. HP-lα was also expressed in the same layers of retina where PAS expression was observed (Fig. lj and Ik), In the cerebellum, expression of PAS was limited to the Purkinje cell layer (Fig. 11 and lm), whereas HP-lα did not show any localized expression throughout the sections (Fig. lj and Ik). Both PAS and HP-lα mRNAs were observed as weak diffuse signal over nonspecific background levels in certain areas of the cerebrum (data not shown). EXAMPLE 4: Coexpression of PAS reduces hypoxia-inducible reporter gene expression in HeLa cells The structural similarity of PAS to hypoxia-inducible transcription factors and the colocalization of PAS and HP-lα in mouse cornea prompted us to investigate the role of PAS in transcriptional control of cellular responsiveness to hypoxia. We performed in HeLa cells a transient transfection assay using a hypoxia-response element- (HRE-) driven luciferase reporter in the absence or presence of transiently expressed PAS. Incubation of the cells under hypoxic (1% O 2 ) conditions induced 4.2-fold activation of the reporter gene, representing the induced transactivation function of endogenous hypoxia-inducible factors (Fig. 2a). Coexpression of PAS reduced hypoxia-inducible reporter gene in HeLa cells stimulated to a high level of luciferase expression in hypoxia-dependent manner (Fig. 2b and Fig. 2c, respectively), indicating that PAS acts as a dominant negative regulator of the function of endogenous hypoxia-inducible factors. PAS had no effect on hypoxia-induced protein stabilization of HP-lα and HLF (Fig. 2d), which has previously been shown to represent a critical initial step in the activation of HP-lα or HLF function 6 7 8 . Thus, PAS seems to inhibit more down-stream steps in signal transduction mediated by hypoxia- inducible transcription factors. EX AMPLE 5: PAS mediates down-regulation of hypoxia-inducible gene expression - PAS impairs interaction between HP-l and the HRE To further investigate the role of PAS in regulation of HP-mediated signaling pathways in hypoxic cells, we generated cells stably overexpressing PAS by stable transfection of mouse hepatoma Hepalclc7 cells. Expression of PAS mRNA in the stably transfected cells was confirmed by Northern blot analysis, whereas the parental Hepalclc7 cells did not show any detectable endogenous PAS expression (Fig. 3a). Wild type Hepalclc7 cells cultured under hypoxic conditions showed markedly increased expression of mRNAs encoding phosphoglycerate kinasel (PGK1) and vascular endothelial growth factor (VEGF) (Fig. 3 a), both of which have been demonstrated to be induced under hypoxic conditions in a variety of cell lines 9 10 . In response to hypoxia, Hepa PAS cells showed decreased levels of induction of these genes (45% and 48% reduction in PGK1 and VEGF activation, respectively; Fig. 3a). PAS-mediated down-regulation of hypoxia-inducible gene expression seemed to be at the transcriptional level, since activation of a transiently transfected HRE-driven reporter gene by hypoxia was significantly lower in Hepa PAS cells than in wild type cells (Fig. 3b). Reporter gene activation was even suppressed in Hepa PAS cells following transient overexpression of HP-lα, indicating that PAS impairs productive interaction between HP-lα and the HRE. EXAMPLE 6: PAS impairs DNA binding activity of the HP-lα/Arnt complex Mobility shift assay was carried out as described by Gradin et al. 12 . In strong support of the results obtained in Example 5, HRE-specific DNA binding activity by the HP-lα/Arnt heterodimeric complex was lower in nuclear extract from either normoxic or hypoxic Hepa PAS cells than corresponding nuclear extracts from wild-type cells (Fig. 3c). EXAMPLE 7: Negative regulation by PAS is specific to HP-mediated signaling pathways. It was examined whether negative regulation by PAS is specific to HP-mediated signaling pathways. The aryl hydrocarbon receptor (AhR), which mediates gene regulation in response to xenobiotic chemicals, is also a member of the bHLH/PAS transcription factor family and shares the dimerization partner factor Arnt with HP-lα 11 . Incubation of the wild-type Hepa lclc7 with 2,3,7, 8-tetrachlorodibenzo-p-dioxin (TCDD) massively induced mRNA expression of the AhR target gene cytochrome P-4501 A1(CYP1 Al). In contrast to hypoxia-inducible gene expression, TCDD-induced expression of CYPl Al mRNA was unperturbed in Hepa PAS cells which showed an induction response similar to that observed in wild type cells (Fig. 3d). Consistent with these data, very similar levels of activation of a xenobiotic response element (XRE)-driven reporter gene by the ligand- stimulated AhR/ Arnt heterodimeric complex 12 was observed both in the wild type and the PAS overexpressing Hepa cells (Fig. 3e). Taken together, PAS seems to preferentially target HP-lα to act as a dominant negative regulator of hypoxia-inducible gene expression. EXAMPLE 8: The inhibitory action of PAS is mediated by direct interaction with HP-lα It was tested whether the inhibitory action of PAS is mediated by direct interaction with HP-lα or Arnt. Radiolabeled, in vitro translated HP-lα or Arnt were incubated with glutathione S-transferase- (GST-) PAS fusion proteins and analyzed by immunoprecipitation assays using anti-GST antibodies (Amersham Pharmacia Biotech). GST-PAS was coprecipitated with HP-lα but not with Arnt, demonstrating specific physical interaction between PAS and HP-lα (Fig. 4a). EX AMPLE 9: The N-terminal part of HP-lα is responsible for the physical interaction with PAS To identify the domain of HP-lα essential for interaction with PAS, we incubated various fragments of HP-lα (see Kallio et al.; ref. 18) fused to the GAL4 minimal DNA binding domain and radiolabeled PAS generated by in vitro translation, and immunoprecipitated this material by anti GAL-4 antibodies (Upstate Biotechnology). GAL4-HP-lα/ 1-826 (full length), /1-330, and /1-652 clearly coprecipitated PAS whereas GAL4-HP-lα/526-826 and GAL4 DBD did not. Together, N-terminal structure of HP-lα mainly composed of bHLH/PAS motif is responsible for the physical association with PAS (Fig. 4b). In support of these observations, mammalian two-hybrid assay employing GAL4- HP-lα/1- 330 and VP16-PAS demonstrated interaction between PAS and N-terminal part of HP- lα in the cells (Fig. 4c). On the other hand, in analogy to the results from pull down assay, GAL-4-PAS and VP 16- Arnt failed to show any interaction (Fig. 4d). EXAMPLE 10: PAS inhibits DNA binding activity of HP-lα/Arnt complex To elucidate the function of PAS/HP- lα complex, Electrophoretic Mobility Shift Assay using HRE oligonucleotide probe and in vitro translated proteins was performed. PAS HP- lα heterodimer, as well as HP-l or PAS by itself, was abortive in binding to HRE. Thus PAS/HP- lα complex seemed to be inactive in mediating expression of the genes under control of HRE. Moreover, DNA binding activity of HP-l /Arnt complex was inhibited by the copresence of PAS but not by the control translation product (Fig. 4e), indicating that the PAS/HP-lα complex might functionally dominate over HP- lα/Arnt DNA binding complex. EXAMPLE 11: Introduction of PAS antisense into cornea cells stimulates expression and hypoxia inducibility of the VEGF gene What is the significance of dominant negative function of PAS in hypoxia signaling and its massive expression in, for example, cornea epithelium? A hallmark of normal cornea is a total avascularity and maintenance of transparency is essential to corneal function. By an overnight eye closure, corneal environment can be enough hypoxic to stimulate hypoxia- inducible gene expression 13 14 , however, neovascularization in cornea is usually prevented although underlying mechanisms are unknown. Given the fact PAS down regulates hypoxia-responsive VEGF expression, we tried to elucidate the effect of PAS on hypoxia-inducible VEGF expression in cornea. For this purpose, a primary culture of the cornea epithelium cells was transfected with antisense PAS expression plasmid (or control empty vector) to manipulate PAS level and incubated with or without hypoxic stimulation for 24 h, thereafter VEGF mRNA expression was monitored in comparison with hepatoma cell lines by Northern Blotting. Hepatoma cell lines showed high level induction of VEGF by hypoxic treatment as previously shown 10 12 (Fig. 5). In sharp contrast, cornea cells transfected with control vector demonstrated low basal level and modest induction of VEGF expression by hypoxia, which might represent the mechanism for a low profile of corneal angiogenesis. Strikingly, introduction of PAS antisense into the cornea cells recovered both basal expression and hypoxia inducibility of VEGF gene (Fig. 5), indicating that PAS may have an important role in silencing angiogenic VEGF expression in cornea especially in hypoxic conditions. REFERENCES 1. Bunn, H. F. & Poyton, R. O. Oxygen sensing and molecular adaptation to hypoxia. Physiol Rev 76, 839-885 (1996). 2. Wenger, R. H. Mammalian oxygen sensing, signalling and gene regulation. J. Exp. Biol. 203, 1253-1263 (2000). 3. Krogh, A., Brown, M., Mian, I. S., Sjolander, K. & Haussler, D. Hidden Markov models in computational biology. Applications to protein modeling. J. Mol Biol. 235, 1501-1531 (1994). 4. Wang, G. L., Jiang, B. H., Rue, E. A. & Semenza, G. L. Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension. Proc NatlAcad Sci USA 92, 5510-5514 (1995). 5. Ema, M. et al. A novel bHLH-PAS factor with close sequence similarity to hypoxia- inducible factor 1 alpha regulates the VEGF expression and is potentially involved in lung and vascular development. Proc Nail AcadSci USA 94, 4273- 4278 (1997). 6. Kallio, P. J., Wilson, W. J., OBrien, S., Makino, Y. & Poellinger, L. Regulation of the hypoxia-inducible transcription factor 1 alpha by the ubiquitin-proteasome pathway. J Biol Chem 274, 6519-6525 (1999). See also International Patent Application WO 00/29437. 7. Maxwell, P. H. et al. The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysis [see comments]. Nature 399, 271-275 (1999). 8. Wiesener, M. S. et al. Induction of endothelial PAS domain protein- 1 by hypoxia: characterization and comparison with hypoxia-inducible factor- lα. Blood 92, 2260- 2268 (1998). 9. Li, H., Ko, H. P. & Whitlock, J. P. Induction of phosphoglycerate kinase 1 gene expression by hypoxia. Roles of Arnt and HP 1 alpha. J Biol Chem 271, 21262- 21267 (1996). 10. Forsythe, J. A. et al. Activation of vascular endothelial growth factor gene transcription by hypoxia-inducible factor 1. Mol. Cell Biol. 16, 4604-4613 (1996). 11. Poellinger, L. in Inducible gene expression (ed. Baeuerle, P. A.) 177-205 (Birkhauser, Boston, 1995). 12. Gradin, K. et al. Functional interference between hypoxia and dioxin signal transduction pathways: competition for recruitment of the Arnt transcription factor. Mol Cell Biol 16, 5221-5231 (1996). 13. Thakur, A., Willcox, M. D. & Stapleton, F. The proinflammatory cytokines and arachidonic acid metabolites in human overnight tears: homeostatic mechanisms. J. Clin. Immunol. 18, 61-70 (1998). 14. Sack, R. A., Beaton, A. R. & Sathe, S. Diurnal variations in angiostatin in human tear fluid: a possible role in prevention of corneal neovascularization. Curr. Eye. Res 18, 186-193 (1999). 15. Ravi, R. et al. Regulation of tumor angiogenesis by p53-induced degradation of hypoxia-inducible factor lα. Genes Dev. 14, 34-44 (2000). 16. Zundel, W. et al. Loss of PTEN facilitates HP-1-mediated gene expression. Genes Dev. 14, 391-396 (2000). 17. Li, J. et al. PR 39, a peptide regulator of angiogenesis. Nat. Med. 6, 49-55 (2000). 18. Norton, J. D., Deed, R. W., Craggs, G. & Sablitzky, F. Id helix-loop-helix protein in cell growth and differentiation. Trends Cell Biol. 8, 58-65 (1998). 19. Eddy, S. R. Multiple alignment using hidden Markov models. Ismb 3, 114-120 (1995). 20. Kallio, P. J. et al. Signal transduction in hypoxic cells: inducible nuclear translocation and recruitment of the CBP/p300 coactivator by the hypoxia-inducible factor-lalpha. Embo J 17, 6573-6586 (1998). 21. Bertilsson, G. et al. Identification of a human nuclear receptor defines a new signaling pathway for C7P3A induction. Proc. Natl. Acad. Sci. USA 95, 12208- 12213 (1998).

Claims: CLAIMS 1. An isolated nucleic acid molecule selected from: (a) nucleic acid molecules comprising a nucleotide sequence set forth as SEQ ID NO: 2; (b) nucleic acid molecules comprising a nucleotide sequence capable of hybridizing, under stringent hybridization conditions, to a nucleotide sequence complementary the polypeptide coding region of a nucleic acid molecule as defined in (a) and which codes for a biologically active mammalian PAS polypeptide or a functionally equivalent modified form thereof; and (c) nucleic acid molecules comprising a nucleic acid sequence which is degenerate as a result of the genetic code to a nucleotide sequence as defined in (a) or (b) and which codes for a biologically active mammalian PAS polypeptide or a functionally equivalent modified form thereof. 2. An isolated mammalian PAS polypeptide encoded by the nucleic acid molecule according to claim 1. 3. The isolated mammalian PAS polypeptide according to claim 2 having an amino acid sequence set forth as SEQ ID NO: 3 in the Sequence Listing 4. A vector comprising the nucleic acid sequence as defined in claim 1. 5. A replicable expression vector, which carries and is capable of mediating the expression of a nucleic acid sequence as defined in claim 1. 6. A cultured host cell harboring a vector according to claim 4 or 5. 7. A process for production of a mammalian PAS polypeptide, comprising culturing a host cell according to claim 6 under conditions whereby said polypeptide is produced, and recovering said polypeptide. 8. A method for identifying an agent useful for activating the expression of a mammalian PAS nucleic acid molecule, said method comprising the steps (i) contacting a candidate agent with a mammalian PAS nucleotide acid molecule according to claim 1; and (ii) determining whether said candidate agent activates the expression of the said mammalian PAS nucleic acid molecule. 9. A method for identifying an agent useful for the inhibition of angiogenesis and/or tumor growth, said method comprising the steps (i) contacting a candidate agent with a mammalian PAS nucleotide acid molecule according to claim 1; and (ii) determining whether said candidate agent activates the expression of the mammalian PAS nucleotide sequence, such activation being indicative for an agent useful for the inhibition of angiogenesis and/or tumor growth. 10. A method for identifying an agent useful for stimulating the biological activities of a mammalian PAS polypeptide, said method comprising the steps (i) contacting a candidate agent with the mammalian PAS polypeptide according to claim 2 or 3; and (ii) determining whether said candidate agent stimulates the biological activities of the said polypeptide. 11. A method for identifying an agent useful for the inhibition of angiogenesis and/or tumor growth, said method comprising the steps (i) contacting a candidate agent with a mammalian PAS polypeptide according to claim 2 or 3; and (ii) determining whether said candidate agent stimulates the biological activities of the said polypeptide, such stimulation being indicative for an agent useful for the treatment of a medical condition related to angiogenesis and/or tumor growth. 12. Use of an agent identified by the method according to any one of claims 8 to 11 in the manufacture of a medicament for the treatment of angiogenic disease or tumor growth. 13. A method for the treatment of angiogenic disease or tumor growth, comprising administering to a subject an effective amount of an agent identified by the method according to any one of claims 8 to 11. 14. The use or method according to claim 12 or 13, wherein said angiogenic disease is related to ischemic cardiovascular lesions, stroke, or diabetic microvascular diseases.

Inventors: BERKENSTAM ANDERS, BERTILSSON GOERAN, POELLINGER LORENZ, BERKENSTAM, ANDERS, BERTILSSON, GOERAN, POELLINGER, LORENZ
Applicants: BERKENSTAM ANDERS, BERTILSSON GOERAN, BIOVITRUM AB, POELLINGER LORENZ, BERKENSTAM, ANDERS, BERTILSSON, GOERAN, POELLINGER, LORENZ
Application year: 2001
Main classification: C07K14
IPC codes: A61K38, A61K45, A61P35, C07K14, C12N1, C12N5, C12N15, C12P21, C12Q1, G01N33