PROCESS FOR THE SYNTHESIS OF OPTICALLY ACTIVE ANTHRACYCLINES

Abstract: It is described a process for the synthesis of optically active anthracyclines characterised by the fact that the key intermediate (R)2-acetyl-2-hydroxy-1,2,3,4-tetrahydronaphtalene 5,8-dialkoxy is prepared from 5,8-dialcoxy-3,4-dihydronaphtalene by acylation asymmetric dihydroxylation, transformation into chloroacetate, dehydrochloridation and final hydrolysis.

Description: PROCESS FOR THE SYNTHESIS OF OPTICALLY ACTIVE ANTHRACYCLINES Field of the invention The present invention refers to a process for the synthesis of optically active anthracyclines wherein the optically active key intermediate (R) 2-acetyl-2- ihydroxy-1,2,3,4-tetrahydronaphthalene 5,8-dialkoxy of formula I. wherein: R = Cι_ 3 alkyl, preferably methyl. is prepared starting from 5,8-dialkoxy-3,4-dihydronaphthalene by acylation, asymmetric dihydroxylation, transformation into chloroacetate dehydrochloridation and final hydrolysis. The invention refers also to the intermediates of formula V e VI: V VI Having an enantiomeric excess higher than 95%. State of the art As it is known the anthracyclines of formula VIII VIII Wherein: R 1 = H, OH, OCH 3 ; R 2 = H, OH; R 3 = X, Y o Z where are compounds having a wide therapeutic use as anti-neoplastic drugs. Known compounds of formula VIII having the above said properties are for example Daunomicin (VIII, wherein: Ri = OCH 3 , R 2 = H, R 3 = X), la Doxorubicin (VIII wherein: Ri = OCH 3 , R 2 = OH, R 3 = X), I'Hydrarubicin (VIII wherein: Ri = H, R 2 = H, R 3 = X) e I'Epirubicin (VIII wherein: R = OCH 3 , R 2 = OH, R 3 = Y), or the compounds described in EP721456, in particular the compound of formula VIII wherein Ri = H, R 2 = OH, R3 = Z, disaccharide anthracycline which is now under clinical development. The synthesis of anthracycline of formula VIM requires many steps and is normally performed starting from an optically active tetraline of formula I which is reacted by a Friedel-Craftsreaction with phthalic anhydride or its derivatives as phthaloyll dichloride or phthaloyll chloride methylester and thereafter cyclised. The so obtained tetracycle is protected in the 13-oxo position with ethylenglycol, is brominated in position 7 and converted into a 7-OH derivative with known methods (see Arcamone et al. , Experientia, 1978,34,1255; Wong et al. Can. J. Chem., 1971 , 49, 2712; Swenton et al. , Tetrahedron, 1984, 40, 4625 ). After deprotection the anthracyclinone of formula VII (wherein R 2 = H) is used as such or is converted into a 14 acyloxy derivative (compound of formula VII wherein R 2 = O- acyl)according to known procedures.. Thereafter the compounds of formula VII are glycosidated with protected mono- or disaccharides as described in literature (see Arcamone et al., Experientia, 1978, 34, 1255; Terashima et al., Bull. Chem. Soc. Jpn, 1986, 59, 423 ) and in EP 6, by deprotection the anthracycline of formula VIII are obtained. In the above described process, or in other similar processes which include as intermediate a tetraline, the key intermediate is the tetraline of formula I itself as above defined. This AB synthon (Wong et al. Can. J. Chem, 1971 , 49, 2712) allows the formation of the corresponding optically active anthracyclinone of formula VII wherein Ri = H, OH, OCH 3 and R 2 = H, OH, O-acyl wherein the acyl group is chosen among formyl, acetyl, mono-, di- or trichloroacetyl, preferably acetyl. VII As above said the compound is finally converted in the desired anthracycline. The stereochemistry of position C-9 of the anthracyclinone is very important for the biological activity of these compounds since only the compounds having (S) configuration in C-9 show an antitumour activity. Therefore also the tetraline intermediate of formula I must obviously possess the same stereochemistry (i.e. an absolute configuration R). The tetraline I is normally prepared. According to the literature, as a racemic mixture starting from 2-acetyl-5,8-dimethoxy tetraline III by oxydrilation in position C-2 with potassium t-butoxide/t-butanole in the presence of oxygen followed by reduction "in situ" (Wong et al., Can. J. Chem., 1971, 49, 2712 ; Gardner et al., J.Org. Chem.1968, 33, 3294). The compound III was prepared, with very low yields by reacting 5,8-dimethoxy- 3,4-dihydronaphthalene II with N-N-diphenylacetamide- POCI3 applying the conditions of the Vilsmeier-Haack reaction followed by the reduction of the double bond. Several attempts of acylating compound II have been reported but all unsuccessful (Rama Rao et al. lnd.J.Chem.1985, 24B, 697). Alternatively the compound III was prepared with a yield of about 50% in 4 st.eps by reaction of 5,8-diacetoxy-3,4-dihydronaphthalene with acetyl chloride/AICb and formation of a chloroacetyl derivative, followed by dehydrochloridation with LiCI, hydrolysis and methylation "in situ" (Russell et al. J. Chem. Soc. Chem. Comm. 1983, 994). Another reaction path for obtaining the precursor III reported in literature includes five steps starting from 5,8-dihydroxy-1,4-diidronphthalene with a total yield of about 50% (Giles et al. S.Afr.J.Chem, 1990,43, 87). The racemic tetraline I is thereafter converted into the pure enantiomeric compound using the normal methods applied for the resolution of racemes through diastereoisomeric Schiff bases on the acetyl lateral chain with (-)-1- phenylethylamine (Arcamone et al. BP 02691/75, 1975). Alternatively the enantiomeric pure compound was prepared by Kinetic resolution via a Sharpless asymmetric epoxidation followed by oxidation of the obtained allyl alcohol obtained by reducing 2-acetyl-5,8-dimethoxy-3,4dihydronaphthalene (Sharpless et al. J.Am.Chem.Soc. 1981, 103, 6237). Another method for obtaining the optically pure tetraline consists in the stereoselective reduction of the racemic mixture with bakers' yeast to diastereoisomeric dioles mixtures followed by chromatographic separation and re-oxidation (Terashima et al., Chem. Pharm. Bull. 1984, 32, 4328). An asymmetric synthesis of the tetraline I starting from precursor III by enantioselective dihydroxylation is described in M. Nakajima et al. Tetrahedron, 1993, 49, 10807, but the several steps and the final excess of tetraline I and especially the use of osmium tetraoxide in stoichiometric quantities, instead of catalytic, quantities and the use of expensive chiral amines (always in stoichiometric quantities) quantities at a temperature of - 110°C, makes very difficult the industrial use of this synthesis. Other asymmetric synthesis of AB synthon using chiral compounds or compounds comprising chiral derivatives of natural compounds are reported in literature but all these synthesis are very complex and unsuitable for industrial application (Krohn, Angew. Chem. Int. Ed. Engl., 1986, 25, 790). Summary of the invention The present invention describes a process for the preparation of anthracyclines of formula VIII as above defined VIII wherein the optically active tetraline of formula I as above defined is stereoselectively prepared starting from 5,8-dialkoxy-3,4- dihydronaphthalene II which, contrary to the methods applying the resolution of racemic mixture, which are difficult to perform, and give yields inferior to 30%, shows the advantage of giving the key intermediate I in yields much higher than those reported in literature and is easily industrially exploitable . In particular, although the literature reported as fruitless, or non interesting because of the low yields, the attempts of acylating compound II ( Rama Rao et al. lnd.J.Chem.1985, 24B, 697, Russell et al. J. Chem. Soc. Chem. Comm. 1983, 994, Giles et al. S.Afr .Chem, 1990,43, 87), the 5,8-dialkoxy-3,4- dihydronaphthalene (compound of formula II wherein R is a group C 1 - 3 alkyl, preferably methyl) can surprisingly be acylated in just one step in the presence of an acyl chloride and aluminium trichloride forming the corresponding acyl derivative III. Moreover this innovative application of the procedure of enantioselective catalytic dihydroxylation of olefins (Sharpless et al., Chem. Rev. 1994, 94, 2483) to give the insature acyl derivative allows to obtain the optically active diol IV in a good yield. The compound is thereafter converted into the corresponding 1 -chloro-2-acetyl-derivative by Sharpless procedure (Sharpless et al, Tetrahedron, 1992, 48, 10515) and dehalogenated following known methods, for example by catalytic hydrogenation or in the presence of tin and a radical precursor or can be directly dehydroxylated by catalytic reduction. The final hydrolysis of the ester group allows the formation of compound I in good yields and high optical purity. Detailed description of the invention In Schema I it is reported a process for obtaining the tetraline of formula I wherein R = CH 3 . In this case the starting product is the 5,8-dimethoxy-3,4- dihydronaphthalene II, obtained by known methods starting from butadiene and p- quinone (Fieser et al., J.Am. Chem. Soc, 1948, 70, 3151). In spite of the fact that in literature the attempts of acylating the compound II were reported as fruitless or no interesting because of the low yields, the 5,8- dimethoxy-3,4-dihydronaphthalene II is treated with acetyl chloride in the presence of an excess of aluminium trichloride, preferably 5 - 9 moles of aluminium trichloride for one mole of acyl chloride, at the temperature of -35° ÷ 25°C, preferably at 0°C. After the usual work-up and crystallisation with ethyl acetate, the product, 2-acetyl-5.8-dimethoxy-3,4-dihydronaphthalene is obtained in yields higher than 70%. Compound III is stereoselectively converted to diol IV by a Sharpless asymmetric dihydroxylation which is described in literature for other olefin substrates (Sharpless et al., Chem. Rev. 1994, 94, 2483). The reactive used in this step is AD-mix α (catalogue Aldrich, reactive 39275-8, see also J. Org. Chem. 1992, 57, 2768) with a further addition of the osmium salt (K 2 OsO 2 (OH) 4 ) and methanesulphonamide. The osmium salt is always in catalytic quantity vis-a-vis the substrate. The reaction is performed at low temperature,-4 e +20°C, preferably at 0°C, the yield is 70%, with an enantiomeric excess higher than 95%. The optically active diol IV is converted into a chloroacetate V through the formation "in situ" of a cycle intermediate using trimethylortoacetate in the presence of an acid catalyst followed by treatment with trimethylsilyl chloride according to a method already described in literature for different dioles (Sharpless et al. Tetrahedron, 1992, 48, 10515). This step gives yields higher then 80%. The reduction of the chloroacetate to acetate VI can be performed photochemical or by thermic treatment in the presence of tributyltinhydride and radical precursors as AIBN or BPO or by catalytic hydrogenation. The yields are higher then 80%. The acetate can be obtained directly by catalytic reduction of diol IV. The hydrolysis of the acetate can be performed with ionic exchange resins in quantitative yield. Alternatively the known methods for the hydrolysis of the acetates, as the treatment with sodium methoxide or sodium hydroxide. What reported in Scheme I can be easily applied to the synthesis of all the compounds of formula I, using the corresponding starting products. The tetraline I which is an object of the present invention is therefore obtained in only 4-5 steps with a total yield much higher than the one reported for the known processes. Moreover, the reaction conditions as described make it possible the industrial scale up of the process. The subsequent steps of the process through the anthracyclinone to the final anthracycline are performed as described in literature. The process according to the invention will be better understood in the light of the hereinafter reported Example which refers to the Scheme 1 i.e. to the preparation of the tetraline of formula I wherein R = CH3. EXAMPLE 1 Synthesis of III To a suspension of aluminium trichloride (449 g) in dichloromethane (2 I) in nitrogen current, acetyl chloride (380 ml) is added drop by drop at 0°C. After 30 min. stirring at 0°C, to the so obtained solution a solution of 5,8-dimthoxy-3,4- dihydronaphthalene II (80 g) in dichloromethane (2,5 I) is slowly added drop by drop. After 30 min stirring at 0°C the mixture was hydrolysed with ice. After separation of the organic phase and washing with HCI 1 N (3 x 6 I), H O (3 x 41) and brine (2 x 4 I), the solvent was evaporated u.v. at 40°C giving a yellow solid residue (98 g). By crystallisation from refluxing ethyl acetate 71 g of the desired compound III where obtained. Yield 73 %. 1 H NMR (CDCI3): 2.44 (s, 3H, H10); 2.53 (m, 2H, H 6 ); 2.80 (m, 2H, H 5 ); 3.30, 3.84 (2s, 6H, OCH3); 6.75 (dd, 2H, H 2 + H 3 ); 7.81 (m, 1H, H 8 ); 13 C NMR (CDCI 3 ): 19.9, 20.5 (C 5 , C 6 ); 25.3 (C10); 55.9, 56.1 (OCH 3 ); 108.5, 113.2 (C 2 , C 3 ); 122.6, 127.2 (C 4a , C 8a ); 131.5 (C 8 ); 137.2 (C 7 ); 150.4, 151.0 (d, C 4 ); 198.8 (C 9 ). TLC: r.f. 0.80 (Petrol ether/Ethyl acetate = 80/20). HPLC: r.t. = 8.9 min (Conditions: Lichrospher 100 RP 18 (5μm, 250 x 4 mm) CH3CN / H2O + 0.1 % TFA = 60 / 40; 1 ml/min; λ = 214 nm; 20 μl of a solution 1 mg/10ml) EXAMPLE 2 Synthesis of IV To a solution of AD mix-α (600 g) and K 2 OsO 2 (OH) 4 (1 g) in water (2 I) f-butanole (2.15 I), methansulphonammide (40.7 g), sodium bicarbonate (109 g) are added. The mixture was stirred up to complete solution of the solid components, cooled down at 0°C, added with 4-acetyl-3,4-dihydronaphthalene (100 g) and vigorously stirred for 96 h. After complete reaction of the precursor, checked by TLC (Petrol ether/ethyl acetate = 80/20), 630 g of sodium bisulphite are added in portions and, after 1 h stirring, 4 I of AcOEt are added and the phases are separated. The organic phase was washed with NaOH 1N (1 x 2 I), H2O (1 x 2 1) and evaporated under vacuum. The obtained solid was solved in 750 ml CH2CI2 and the solution was extracted with H2SO4 3 % saturated with K2SO4 (4 x 200 ml), NaHCO3 s.s. (1 x 300 ml) and H2O (1 x 300 ml). The organic phase, dried on anhydrous MgSO4, was evaporated under vacuum leaving a solid residue. The product was crystallised from AcOEt / cyclohexane = 1/1, filtered and dried under vacuum. 78.7 g of a crystalline solid were obtained. Yield: 70.5% 1 H NMR (CDCI3): 1.87 (m, 2H, H 6 ); 2.38 (s, 3H, H 10 ); 2.79 (m, 2H, H 5 ); 3.78, 3.84 (2s, 6H, OCH3); 3.81 (m, 1H, H 8 ); 4.87 (d, 1H, OH 8 ); 5.29 (s, 1H, OH 7 ); 6.71 (s, 2H, H 2 + H 3 ). 13 C NMR (CDCI3): 19.1 (C 10 ); 25.8 (C 5 ); 28.7 (C 6 ); 55.7, 55.7 (OCH 3 ); 68.5 (C 8 ); 78.7 (C 7 ); 108.0, 108.8 (C 2 , C 3 ); 125.8, 127.1 (C 4a , C 8a ); 151.1 , 152.3 (Cι, C 4 ); 214.2 (C 9 ). TLC: r.f. 0.25 (Petrol ether/Ethyl acetate = 80/20) HPLC: r.t. = 4.1 min (Conditions: Lichrospher 100 RP 18 (5μm) 250 x 4 mm CH3CN / H2O + 0.1 % TFA = 50 / 50; 1 ml/min; λ = 214 nm; 20 μl of a solution 2.8 mg/10ml) e.e. = 98 % determined by chiral HPLC (Conditions: Chiralcel OD 250 X 4.6 mm; n-hexane / EtOH = 90 / 10; 1 ml/min; λ = 214 nm; 20 μl of a solution 1.3 mg / 10 ml) m. p. = 141-143°C. [α] D 25 = -21.9° (c = 1.0, CHCI3) EXAMPLE 3 Synthesis of V To a solution of diol (77 g) in CH2CI2 (600 ml), under nitrogen, trimethylortoacetate (59.3 ml) and piridiniumtoluene-4-sulphonate (2 g) are added. The solution is stirred at room temperature for 24 H. The solvent is evaporated under vacuum leaving a solid residue. The solid was solubilised CH 2 CI 2 (600 ml) and added, under nitrogen, with trimethylsilyl chloride (65 ml). The reaction mixture is stirred at room temperature for 1 h and, after evaporation of the solvent under vacuum, was treated with cyclohexane (400 ml) under vigorous stirring for 3 h. The solid was filtered and dried under vacuum. 98.1 g of the desired product were obtained (quantitative yield). 1 H NMR (CDCI3): 1.96 (s, 3H, H 10 ); 1.97 - 3.15 (m, 4H, H 5 + H 6 ); 2.43 (s, 3H, H12); 3.81, 3.87 (2s, 6H, OCH 3 ); 5.35 (d, 1H, H 8 ); 6.76 (dd, 2H, H 2 + H 3 ); 13 C NMR (CDCI3): 19.6 (C11); 20.2, 20.5 (C 5 , C 6 ); 26.3 (C 12 ); 52.9 (C 8 ); 55.6, 56.0 (OCH3); 82.9 (C 7 ); 108.3, 110.1 (C 2 , C 3 ); 123.2, 125.4 (C 4a , C 8a ); 150.7, 151.6 (d, C 4 ); 169.6 (C11); 204.2 (C 9 ). TLC : r.f. = 0.55 (Petrol ether / AcOEt = 75 / 25) m.p. = 128-138°C. [α] D 25 = -16.2° (c = 1.0, CH2CI2). EXAMPLE 4 Synthesis of VI To a solution of chloacetate (97.3 g) in toluene (2 I) AIBN (1.5 g) and tributyl- tinhydrure (225 ml) were added in nitrogen current. The mixture was stirred under the light of a 200 Watt wolfram lamp for 24 h and thereafter extracted with water (500 ml). The organic phase is separated, dried and evaporated under vacuum. The residue is treated with cyclohexane (500 ml) under stirring, filtered and dried under vacuum at 40°C. 67.8 g of the desired product are obtained in the form of a white solid. Yield : 80.3 %. 1 H NMR (CDCI3): 1.95, 2.50 (2m, 2H, H 6 ); 2.05 (1.3H, H 10 ); 2.22 (1.3H, H 12 ); 2.40, 2.90 (2m, 2H, H 5 ); 3.00 (dd, 2H, H 8 ); 3.77, 3.80 (2s, 6H, OCH 3 ); 6.66 (m, 2H, H 2 + H 3 ). 13 C NMR (CDCI3): 19.5, 21.0 (C 5 , C 6 ); 24.0 (C 10 ); 26.7 (C 12 ); 30.2 (C 8 ); 55.6, 55.5 (OCH 3 ); 83.6 (C 7 ); 107.0, 107.2 (C 2 , C 3 ); 122.7, 125.1 (C 4a , C 8a ); 150.9, 151.4 (d, C 4 ); 170.5 (Cn); 206.5 (C 9 ). TLC : r.f. = 0.28 (Toluene/Ethylacetate = 95/5) HPLC: r.t. = 7.4 min (Conditions: Lichrospher 100 RP 18 (5μm) 250 x 4 mm, CH3CN / H2O + 0.1 % TFA = 60 / 40; 1 ml/min; λ = 214 nm; 20 μl of a solution 1.2 mg / 10 ml) m.p.: 110-118°C. [α] D 25 : -46.3° (c = 1.0, CHCI3). EXAMPLE 5 Synthesis of I To a solution of acetate (66 g) in methanol (5 I) the Amberlite IRA-400 resin (OH) (183 ml) previously activated by treatment with NaOH 30% (8 x 400 ml) and washed with water (5 x 400 ml) and methanol (4 x 400 ml) was added. The reaction mixture is stirred for a night at room temperature. After removal of the resin by filtration and evaporation of the solvent under vacuum a solid residue was obtained which after crystallisation from cyclohexane/ethylacetate, filtration and drying gave 51.85 g of desired product. Yield: 92 % . 1 H NMR (CDCI3): 1.89 (m, 2H, H 6 ); 2.33 (s, 3H, H10); 2.91 (m, 4H, H 5 + H 8 ); 3.65 (s, 1H, OH); 3.77, 3.80 (2s, 6H, OCH 3 ); 6.66 (s, 2H, H 2 + H 3 ). 13 C NMR (CDCI 3 ): 19.2 (C 5 ); 23.9 (C 10 ); 29.7, 32.4 (C 6 , C 8 ); 55.5, 55.6 (OCH 3 ); 76.4 (C 7 ); 107.0, 107.4 (C 2 , C 3 ); 122.7, 125.5 (C 4a , C 8a ); 151.1 , 151.6 (d, C 4 ); 212.3 (C 9 ). TLC: r.f. = 0.27 (Petrol ether/Ethylacetate = 80/20 HPLC : r.t. = 5.9 min (Conditions: Lichrospher 100 RP 18 (5μm) 250 x 4 mm, CH3CN / H2O + 0.1 % TFA = 50 / 50; 1 ml/min; λ = 214 nm; 20 μl of a solution 2.5 mg/ml) e.e = > 99% determined by chiral HPLC (Conditions: Chiralcel OD 250 X 4.6 mm; n-hexane I EtOH = 90 / 10; 1 ml/min; λ = 214 nm; 20 μl of a solution 1.35 mg / 10 ml) m.p. : 126-129°C. [α] D 25 = -46.2° (c = 1.0, CHCI3) SCHEME I

Claims: Claims 1. Process for the preparation of anthracycline of formula VIII: VIII wherein Ri = H, OH, OCH 3 ; R 2 = H, OH; R 3 = X, Y or Z where: comprising the formation of the intermediate of formula (I): I wherein: R = C 1 - 3 , alkyl characterised in that said intermediate of formula (I) is prepared starting from a 5- dialkoxy-3,4-dihydronaphthalene of formula (II): wherein R = C 1 - 3 alkyl 2. Process according to claim 1, wherein: a) the 5,8-dialkoxy-3,4-dihydronaphthalene of formula II, according to Claim 1, is acylated in a single step forming the corresponding acyl derivative (III) Wherein R = C1-3 alkyl b) the acyl derivative (III) is enantioselectively hydroxylated to form the diol (IV) IV Wherein R = Cι- 3 alkyl Which is transformed by known methods in the tetralin of formula (I) as defined in claim 1, c) the above said tetralin is converted into the anthracyclinone of formula VII VII wherein Ri = H, OH, OCH 3 , R 2 = H, OH, O-acyl where the acyl is chosen among formyl, acetyl, mono-, di- or tri-chloroacetyl and this is finally converted into the anthracycline of formula VIII, as defined in Claim 1, by known techniques. 3. Process according to claim 2 wherein R = methyl and R 2 = acetyl. 4. Process according to claims 2 and 3 wherein the acylation of (II) to (III) is performed in acetyl chloride in the presence of an excess of aluminium chloride at -35°C - + 25°C. 5. Process according to claim 4 wherein the acylation is performed at 0°C. 6. Process according to claims 1 - 5 wherein the enantioselective dihydroxylation according to step (b) is performed using catalytic quantities of osmium using the reactive AD-mix α with a further addition of an osmium salt and methanesulphoneamide. 7. Process according to claim 6 wherein the osmium salt used is K 2 OsO 2 (OH)4. 8. Process according to claim 7 wherein the reaction is performed at -4 - +20°C. 9. Process according to claim 8 wherein the reaction is performed at 0°C. 10. Process according to claims 1 - 9 wherein the anthracyclines of formula (VIII) are: daunomicin (VIII, wherein Ri = OCH 3 , R 2 = H, R 3 = X), doxorubicin (VIII wherein: Ri = OCH 3 , R 2 = OH, R 3 = X), i'hydrarubicin (VIII wherein: Ri = H, R 2 = H, R 3 = X) I'epirubicin (VIM, wherein: Ri = OCH 3 , R 2 = OH, R 3 = Y), or a tetracycline of formula VIII wherein: Ri = H, R 2 = OH, R 3 = Z, where X, Y and Z are as defined in claim 1. 11. Optically active compound of formula V V 12. Optically active compound of formula VI VI

Inventors: CROTTI PAOLO, D ARATA FABIO, DI BUGNO CRISTINA, RAMACCIOTTI ALESSIO, CROTTI, PAOLO, D'ARATA, FABIO, DI BUGNO, CRISTINA, RAMACCIOTTI, ALESSIO
Applicants: ARATA FABIO D, BUGNO CRISTINA DI, CROTTI PAOLO, MENARINI RICERCHE SPA, RAMACCIOTTI ALESSIO, CROTTI, PAOLO, D'ARATA, FABIO, DI BUGNO, CRISTINA, MENARINI RICERCHE S.P.A., RAMACCIOTTI, ALESSIO
Application year: 2001
Main classification: C07H15
IPC codes: C07B61, C07C69, C07H15