FORMULATION FOR CROP PROTECTION WITH INSECTICIDAL AND ACARICIDAL ACTION

Abstract: The invention concerns a new crop protection formulation possessing insecticidal and acaricidal action, selected for use in agriculture and horticulture. The formulation has the following composition expressed in weight percent: 0.2-0.3 active compound from the avermectins group equalized to the biological activity of avermectin B1; 5.0-30.0 surfactant; 40.0-80.0 solvents; 5.0-30.0 additional solvents; 1.0-6.0 benzyl alcohol; 0.1-1.0 antioxidants; 1.0-3.0 adhesives. It is used as a 0.10-0.12% water solution and demonstrates high biological efficiency against agriculture and horticulture pests.

Description: FORMULATION FOR CROP PROTECTION WITH INSECTICIDAL AND ACARICIDAL ACTION Techniques field of application The invention concerns' a new crop protection formulation possessing insecticidal and acaricidal action, selected for use in agronomic and horticultural crops cultivated in greenhouses and in the open. Background of the invention Recently, avermectin and its major component avermectin Bi (B Id + B lb ), with the common name abamectin [1], synthesized of the fermentation of Strepiomyces avermiiϊlis has been widely used against pests in agronomic and horticultural crops. The avermectin formulations are a new generation of insecticides with a mechanism of action differing from that of the chemical pesticides since avermectin Bi (abamectin) is effective against populations of insects and acarines which have developed resistance to organophosphoms compounds, carbamates, pyrethroids, etc. [2]- Abamectin formulations are known which proved to be effective against greenhouse and tobacco white fly, western flower thrips, leafminer flies. [3] Studies carried out in Bulgaria report the high efficacy of abamectin against leafminer flies from the Liriomyza order, the Colorado potato beetle (Leptinotarza decemlineata), the greenhouse whitefly (Trialeurodes vaporariorum), the two- spotted spider mite (Tetranychus urticae) and the peach aphid (Myzus percae).[4] Description of the invention The invention concerns a new crop protection formulation possessing insecticidal and acaricidal action, selected for use in agriculture and horticulture. The novel formulation has the following composition, expressed in percent weight units: 0.2 - 3.0 of the 'active component of the avermectins group (including abamectin, ivermectin, natural mycelium product) equal to the biological activity of avemiectin B^ 5.0 - 30.0 surfactant (emulsifier) from the polyoxyhydrogenated castor-oil group (Kremofor PH-40 or Kremofor EL-35); 40.0 - 80.0 solvents - propylene glycol or polyethylene glycol; 5.0 - 30.0 other solvents - dimethyl acetamide or dimethyl sulfoxide; 1.0 - 6.0 benzyl alcohol; 0.1 - 1.0 antioxidants - butylhydroxytoluene or butylhydroxyanisol; 1.0 - 3.0 adhesives - poly vinyl pyrolidon (Colydon). Water solution of the formulation with a concentration of 0.10 - 0.12% is applied so that very good foliage covering is assured. The formulation components are stable. The biological studies report good efficacy against Colorado, potato beetle (Leptinotarza decemlineata), the greenhouse whitefly (Trialeurodes vaporiorum), the leafminer fly (Liriomyza huidobrensis), the two-spotted spider mite (Tetranychus urticae) and others. Very high efficacy' against Colorado potato beetle (90 - 100%) was reported on the 7 th day of treatment, veiy good control was reported against leafminer fly (80 - 99%) and greenhouse whitefly (80 - 84%). Examples The examples below are provided in order that the invention might be more fully understood. They should not be construed as limitative of the invention: Example 1: Composition of the formulation Content Abamectin 100%) 0.2 g Dimethylacetamide 5.0 g Kremofor RH-40 5.0 g Benzyl alcohol 1.0 g Butylhydroxytoluene 0.05 g Colydon 1.0 g Propylene glycol up to 100.0ml Example 2: Composition of the formulation Content Abamectin 100% 3.0 g D imethy lacetamide 30.0 g Kremofor RH-40 30.0 g Benzyl alcohol 2.0 g Butylhydroxytoluene 0.2 g Colydon 4-0 g Propylene glycol up to 100.0ml Example 3: Composition of the formulation Content Abamectin 100% 0.2 g Dimethyl sulfoxide 5.0 g Kremofor EL-35 5.0 g Benzyl alcohol 1.0 g Butylhydroxyanisol 0.05 g Colydon 1.0 g Propylene glycol up to 100.0 ml Example 4: Composition of the formulation Content Abamectin 100% 3.0 g Dimethyl sulfoxide 30.0 g Kremofor EL-35 30.0 g Benzyl alcohol 3.0 g Butylhydroxyanisol 0.2 g Colydon 4.0 g Propylene glycol up to 100.0 ml Example 5: Composition of the formulation Content Natural concentrate, expressed as avermectin B \ 0.2 g Dimethyl sulfoxide 5.0 g Kremofor RH-40 5.0 g Butylhydroxyanisol 0.05 g Benzyl alcohol 2.0 g Colydon 1.0 g Propylene glycol up to 100.0 ml Example 6: Composition of the formulation Content Natural concentrate, expressed as avermectin B] 3.0 g Dimethylacetamide 30.0 g Cremophor EL-35 30.0 g Butylhydroxytoluol 0.2 g Benzyl alcohol 3.0 g Colydon 4.0 g Propylene glycol up to 100.0 ml Application of the invention Studies for assessment of the biological efficacy of the novel formulation have been carried out as described in Examples 1 and 2. Efficacy of the formulation against Colorado potato beetle (Leptinotarza decemlineata) The Colorado, potato beetle is an important pest in potato and aubergine cultures which is due to its consistent proliferation, the long-lasting active feeding of the larvae and the adults and its high reproductive capacity. Field and laboratory trials were performed to assess the efficacy of the formulation. The novel formulation proved to be toxic to all larval stages; mortality was provided in the . early-stage larvae. Fourth stage larvae and imago that remained unaffected by some commonly used preparations showed fair sensitivity to the new formulation that was applied at a concentration rate of 0.1%. The first visible symptoms of exposure to the formulation were reduced food willingness and paralysis. Stomach intoxication symptoms appeared such as flow out of brown liquid from the mouth and excretion of splashy to liquid feces which were more frequent in adult stages and imago. Mortality of the early larval stages was reported over a short period of , 10 to 15 hours. Two to three days after the treatment the adults and imago moved slightly their hind legs and their mouth showing signs of partial though irrevocable paralysis. After killing the bodies of the early-stage larvae went darker, whereas red-orange coloring of the adults remained- for several days and afterwards turned dark brown. Parts of the thin wings of the insects were visible at the end of the stomach.. The formulation was shown to be veiy effective - on the third day after spraying the efficacy was 95% and on the l day it achieved 100%o (Table 1). Table 1. Efficacy of the formulation against leafminer fly (Liriomyza huidobrensis) The control of leafminer fly populations proved to be very difficult because of the secretive way of life and feeding habits of their larvae. Trials were carried out on gerbera daisy and chrysanthemum sprouts and flowers and vegetable cultui'es of tomatoes and cucumbers. The application rate of the formulation was 0.12%. The larval kill in the mines was the main criterion to evaluate the formulation efficacy. The leafminer development was completely inhibited after 2 or 3 sprayings with a 7- day interval' regardless of the density of the population. The biological activity of the leafminer fly was totally suppressed after 2 - 3 sprayings with a 7-day interval in all degrees of density of the cited cultures. A biologic efficacy of 80 to 99%) was provided by the novel formulation against leafminer fly (Table 2). Table 2. Efficacy of the formulation against greenhouse whitefly (Trialeurodes vaporariorum) The greenhouse whitefly is an important worldwide pest which attacks greenliouse and open-air cultures. Its larvae, nymphs and adults feed on the foliage underlayer. hi laboratory and field studies the formulation at a concentration of 0.12% killed 84%> of the larvae, eggs and adults of the greenliouse whitefly in vegetable and ornamental cultures (Table 3). Table 3. REFERENCES: 1. Campbell W.C, Springer-Nerlag, Ed."Ivermectin and Abamectin", New York, 1989 2. Paιτella,1996; Grasselly, 1996; Roditanis, 1997. 3. Dybas K.A. 1989, Abamectin use in crop protection in "Ivermectin and Abamectin, Ed W.C. Campbell, Springer-Nerlag. 4. Egerton J.E. et al. 1979. Avermectins, a new family of potent anthelmintic agents. Efficacy of the B Ia component. "Antimicr. Agents Chemother ", 15, 372-378. 5. Green A, Dybas K. A. 1984, Avermectin B la control of mites on ornamentals, Proc. British Crop.Prot.Conference, Λ-O1.3, 1129-1130. 6. Niktorov et al, Pesticides, 1996

Claims: Claims 1. A formulation for crop protection possessing an insecticidal and acaricidal action with the following composition in weight percent: 0.2 - 0.3 active compound from the avermectins group equalized to the biological activity of avermectin Bi; 5.0 - 30.0 surfactant; 40.0 - 80.0 solvents; 5.0 - 30.0 additional solvents; 1.0 - 6.0 benzyl alcohol; 0.1 - 1.0 antioxidants; 1.0 - 3.0 adhesives. 2. The formulation of claim 1, wherein the formulation is composed of an active substance from • the avermectins group, comprising of abamectin, ivermectin and a natural mycelium product. 3. The formulation of claim 1, wherein emulsifiers from the polyoxyhydrogenated castor-oil group (Kremofor RH-40 and Kremofor EL-35) are used as a surfactant. 4. The formulation of claim 1, wherein propylene glycol and polyethylene glycol are used as solvents. 5. The formulation of claim 1, wherein dimethylacetamide and dimethylsulfoxide are used as additional solvents. 6. The formulation of claim 1, wherein butylhydroxytoluene and butylhydroxyanisol are used as antioxidants 7. The formulation of claim 1 wherein polyvinilpyrolidon (Colydon) is used as adhesive. 8. Application of the formulation according to claims 1 to 7 as a crop protection product in th&form of 0.10-0.12%) water solution.

Inventors: ATANASOVA GINKA SHTEREVA, DIMITROVA EKATERINA LOGINOVA, KARANIKOLOVA MARIANA IVANOVA, PAMIHOVA ZOIA IVANOVA, VIDENOVA EVGENIA STOIANOVA, ATANASOVA, GINKA SHTEREVA, DIMITROVA, EKATERINA, LOGINOVA, KARANIKOLOVA, MARIANA IVANOVA, PAMIHOVA, ZOIA IVANOVA, VIDENOVA, EVGENIA STOIANOVA
Applicants: ATANASOVA GINKA SHTEREVA, BIOVET JSC, DIMITROVA EKATERINA LOGINOVA, KARANIKOLOVA MARIANA IVANOVA, PAMIHOVA ZOIA IVANOVA, VIDENOVA EVGENIA STOIANOVA, ATANASOVA, GINKA SHTEREVA, DIMITROVA, EKATERINA, LOGINOVA, KARANIKOLOVA, MARIANA IVANOVA, PAMIHOVA, ZOIA IVANOVA, VIDENOVA, EVGENIA STOIANOVA
Application year: 2001
Main classification: A01N43
IPC codes: A01N43, A01N63