XENOGENEIC ANTIBODY CONTAINING PLASMA REAGENTS AND ASSOCIATED METHODS

Abstract: A bioassay reagent is provided consisting of a xenogeneic antibody containing plasma. Specifically, the bioassay reagent disclosed contains plasma from a first animal species and antibodies derived from at least one second different animal species that recognize antigens indigenous to the first animal species. More specifically, a bioassay for assessing the efficacy of therapeutics intended to treat patients having autoimmune diseases, specifically, hemophiliacs having anti-blood factor antibodies (inhibitory factors) present in their blood. Also provided are associated methods for making and using the bioassay reagents disclosed.

Description: XENOGENEIC ANTIBODY CONTAINING PLASMA REAGENTS AND ASSOCIATED METHODS FIELD OF THE INVENTION The present invention provides plasma derived from a first animal species that contains antibodies from a second animal species (xenogeneic antibodies). Specifically, the present invention provides xenogeneic antibody containing plasma that is useful as biological assay (bioassay) reagents. More specifically, the present invention provides a bioassay reagent, methods for making the reagent and methods for using the reagent in an assay intended to determine the biological activity of compounds used to treat autoimmune and hematological disorders BACKGROUND OF THE INVENTION One of the most common hematological disorders is hemophilia. Hemophilia is a disease characterized by blood clotting (hemostasis) disorders caused by deficiencies in coagulation factors. Hemostasis is a complex biological process involving numerous diverse enzymes, co-factors, macromolecules and cell signaling compounds. Hemophilia can be inherited or acquired and both vary in severity. Inherited hemophilia usually involves only one hemostatic component or pathway whereas acquired hemophilia generally involves multiple hemostatic pathways Generally, hemophilia is treated using coagulation factor concentrates derived from donor plasma or through recombinant DNA technologies. The most frequently used replacement coagulation factors include Factor V, Factor NTH, von Willebrand Factor (vWF), Factor IX, and Factor X. One of the most serious side effects of treating hemophilia with replacement coagulation factor concentrates is the development of inhibitory antibodies against one or more of these coagulation factors. Generally, the development of inhibitory antibodies by coagulation factor recipients is a rare event. In hemophilia type B (Christmas Disease) less than 1% of patients develop inhibitory antibodies. However, some reports have estimated that 45% of all hemophilia type A patents have some inhibitory antibodies. Moreover, inhibitory factors can be associated with various autoimmune diseases including systemic lupus erythematosus (SLE). However, the inhibitory factor found in SLE patients is not directed at a specific coagulation factor, but rather it is an anti-phospholipid antibody that non-specifically interferes with many coagulation pathway reactions. Generally, inhibitory antibodies form when the recipient's immune system reacts to the replacement coagulation factor as it would a foreign substance (antigen) such as a virus. In response to the antigen, antibodies that react with and neutralize the replacement coagulation factors are formed. Hemophiliacs that develop inhibitory antibodies against specific coagulation factors can no longer be effectively treated using preparations containing these replacement-clotting concentrates. Consequently, significant efforts have been invested in developing treatments for patients having inhibitory antibodies. One of the most successful blood clotting deficiency therapies for treating bleeding episodes in inhibitory antibody positive patients is Factor Eight Inhibition Bypassing Activity (FEIBA). FEIBA is made from pooled human plasma and contains Factor II, activated Factor Nil, Factor VII, Factor IX and Factor X. FEIBA bypasses the need for Factors NIII and IX by acting simultaneously at different points in the blood coagulation cascade (hemophilia A patients most often have Factor NIII inhibitor whereas hemophilia B patents most often have Factor IX inhibitor). All biological reagents and therapeutics must be stringently evaluated prior to their in vitro diagnostic or in vivo therapeutic use. In the past, anti-inhibitory blood clotting therapeutics have been evaluated in vivo using experimental animals such as hemophilic dogs, which exhibit a genetic defect similar to human hemophilia, or animals in which a transient hemophilia has been established by administering blood coagulation factor antibodies to such animals. However, side effects from hemophilia make these animal models difficult to establish and maintain. Moreover, animal models do not completely simulate human hemophilia and/or may show biased results due to normalization of the transient hemophilic state. Examples of successful animal models for human blood coagulation disorders are described in J.Clin. Invest 82 (1988), 206-211, WO 95/01570 and AT 404 429 B. Therefore, non-human animal test systems have proven to be less than ideal for evaluating the therapeutic efficacy of anti-clotting factor inhibitory antibody treatments. A blood clotting deficiency therapeutic's efficacy can be accurately evaluated in vitro providing adequate standards have been established in vivo and sufficeient consistant testing reagents having appropriate sensitivity and specificity are available. Current in vitro testing reagents are generally derived from patients' serum or plasma (herein after referred to collectively as plasma) that exhibit specific blood coagulation disorders. Such plasma are, especially samples from patients having inhibitor antibodies, are rare. Consequently, standardized testing reagents derived from human plasma are extremely difficult to provide on a consistent basis. Moreover, blood borne pathogen contamination is common in donor plasma derived from hemophiliacs. Many hemophiliacs have used blood factor concentrates derived from pooled human plasma for decades. Consequently, they may have been exposed to products contaminated with human immunodeficiency virus (HIN), human T-cell lymphotropic virus type I (HTLN I), human T-cell lymphotropic virus type II (HTLN II), hepatitis virus type B 5 hepatitis virus type C and other, potentially unidentified viral and/or non-viral pathogens. Therefore, not only is human plasma having blood-clotting deficiencies rare, especially inhibitory antibody disorders, but available sources are also frequently contaminated with infectious agents. Consequentially, there is a need for constant, well characterized, non-infectious human plasma suitable for in vitro testing of therapeutic argents intended to treat blood clotting disorders, autoimmune diseases, and especially, anti-clotting factor inhibitory antibody therapeutics. SUMMARY OF THE INVENTION It is therefore an object of the present invention to provide an in vitro bioassay reagent for determining the efficacy of a therapeutic agent. It is another object of the present invention to provide an in vitro bioassay reagent, an in vitro test method and kit useful for testing autoimmune disease therapeutics. It is yet another object of the present invention to provide an in vitro bioassay reagent containing human plasma that eliminates the risk of exposure to blood borne infectious agents such as human immunodeficiency virus (HIN), human T-cell lymphotropic virus type I (HTLN I), human T-cell lymphotropic virus type II (HTLV II), hepatitis virus type B, and hepatitis virus type C. Another object of the present invention is to provide an anti-blood clotting factor antibody containing plasma suitable of the in vitro testing of therapeutics used to treat hemophiliacs having anti-blood clotting factor antibodies (inhibitory antibodies). In its broadest sense the present invention is a bioassay reagent made by blending the plasma of one animal with antibodies derived from at least one other different animal (xenogeneic antibody containing plasma). The xenogeneic antibodies recognize antigens indigenous to the animal used as the plasma source (the first animal species). The normal, healthy animal immune system generally does not recognize indigenous antigens, consequently, these antigens must be inoculated into a second animal species to produce the xenogeneic antibodies of the present invention. Suitable antigens recognized by the xenogeneic antibodies of the present invention include, but are not limited to lipids, phospholipids, glycolipids, proteins, amino acids, glycoproteins, carbohydrates, and nucleic acids. In one embodiment of the present invention the antigens recognized by the xenogeneic antibodies are blood coagulation factor antigens including, but not limited to Factor N, Factor VIII, Factor IX, factor X and von Willebrand Factor. Any animal species can serve as the plasma source. Generally, the plasma source is derived from the animal to be treated using the therapeutic that the present invention is intended to test. For example, in one embodiment of the present invention the bioassay is intended to test the efficacy of therapeutics used to treat human autoimmune diseases, therefore, a human (the first animal, order Primate) is the optimum plasma source. Other suitable animal orders include, but are not limited to Artiodactyla, Perissodactyla, Rodentia, and Lagomorpha. The xenogeneic antibody is produced in a second animal species for reasons briefly explained above. Suitable second animal orders include, but are not limited to Artiodactyla, Perissodactyla, Rodentia, Lagomorpha and Primates, providing that the second animal species is different from the first animal species, and that the second animal will immunologically recognize the antigens administered. In one embodiment of the present invention a goat (order Artiodactyla, species Capra) is used to produce the xenogeneic antibodies of the present invention. Suitable Capra species include, but are not limited to aegagrus, ibex, pyrenaica, cylindricornis, hircus, andfalconeri. Thus, the present invention is directed to a bioassay reagent comprising plasma of a first animal species that contains antibodies from at least one other animal species (xenogeneic antibodies). These xenogeneic antibodies are directed against antigens indigenous to the species from which the plasma component was derived. In one embodiment of the present invention autoimmune antibodies present in the plasma component are removed (immunodepleted) before the xenogeneic antibodies are added. The present invention provides a consistent, standardized bioassay reagent that is ideally suited for testing autoimmune therapeutics for efficacy prior to use in animals. Moreover, the bioassay reagents of the present invention can be prepared using human plasma that is free of detectable blood borne infectious agents The present compositions are easy to produce using methods known to those of ordinary skill in the art of immunology (Thromb. Haemost. 68 (2) (1992), 155-159; British Journal of Hematology 46 (1980) 471-481). However, the combination of normal human plasma (the first animal species) containing xenogeneic antibodies from a second animal species such as, but not limited to cattle, horses, goats, rabbits, mice, and rats that recognized antigen indigenous to the fist animal species is not known. Consequently, the present invention provides novel methods and reagents for the in vitro efficacy analysis of therapeutics using xenogeneic antibody containing plasma. BRIEF DESCRIPTION OF THE DRAWINGS FIG.1 graphically compares the clotting time reduction of (a) human Factor NIII deficient plasma containing naturally occurring auto antibodies against Factor VIII (patient plasma containing inhibitory antibodies), (b) human Factor VIII deficient (immunodepleted) plasma with added inhibitor against Factor VIII (the xenogeneic antibodies of the present invention) and (c) human normal plasma with added inhibitor against Factor VIII (the xenogeneic antibodies of the present invention). DETAILED DESCRIPTION OF THE INVENTION The present invention is generally directed at bioassay reagents consisting of normal plasma from one animal species that is mixed with antibodies derived from at least one other different animal species (xenogeneic antibodies) to form a xenogeneic antibody containing plasma. Specifically, the bioassay reagents disclosed contain plasma from a first animal species and antibodies derived from at least one second different animal species that recognize antigens indigenous to the first animal species. More specifically, a bioassay for assessing the efficacy of therapeutics intended to treat patients having auto-immune diseases, specifically, hemophiliacs having anti- blood factor antibodies (inhibitory factors) present in their blood. Also provided are associated methods for making and using the bioassay reagents disclosed. Generally, the proteins, glycoproteins, lipids, glycolipids, carbohydrates, nucleic acids and other structural components (antigens) of a normal animal's body do not elicit a humoral immune response (that part of the immune system made of plasma cells that produce antibodies). That is, our immune systems generally do not make antibodies against ourselves. However, in rare cases an animal's immune system will produce antibodies against its own antigens resulting in "autoantibodies" and an "autoimmune" disease. In severe cases, aggressive autoimmune diseases can be life threatening. Autoantibodies generally form in one of several ways. The most common cause of autoimmune diseases is genetically based. The immune systems of genetically predisposed persons respond to their own antigens as if they were foreign antigens and form antibodies against them. Systemic lupus Erythematosus (SLE) is an example of a genetically based autoimmune disease where the body's humoral immune system forms autoantibodies against its own nucleic acids, nuclear proteins, and phosolipids (40% to 60% of SLE patents form anti-phospholipid antibodies). Autoimmune disease can also be drug induced, or the result of prolonged immune system stimulation resulting from repeated injections of therapeutics derived from a closely related species. An example of the latter are hemophiliacs that receive blood clotting factor concentrates derived from pooled human plasma that produce antibodies against blood clotting factors (inhibitory antibodies) including, but not limited to, Factor VIII, Factor IX, Factor X, von Willebrand Factor (vWF), and/or phospholipids. The induction of an immune response against an animal's own antigens is an uncommon event and thus supplies of human plasma having autoantibodies are rare. Moreover, autoantibody containing human plasma, especially plasma containing inhibitory factor, has a high incidence of blood borne pathogen contamination. These pathogens include, but not limited to human immunodeficiency virus (HIN), human T-cell lymphotropic virus type I (HTLV I), human T-cell lymphotropic virus type II (HTLV II), hepatitis virus type B, hepatitis virus type C and other, potentially unidentified viral and/or non- viral pathogens. Therefore, the ability to produce large quantities of plasma containing autoantibodies free from detectable blood borne pathogen would be a significant scientific advance. However, inducing an immune response against an animal's own antigens remains the most significant obstacle to success. The inventors of the present invention have overcome the aforementioned obstacle by devising a system whereby detectable blood borne pathogen free animal plasma from one species (the plasma donor) is mixed with antibodies that react with antigens indigenous to the plasma donor. In order to produce the desired antibodies, antigens from the plasma donor are extracted from the plasma donor species and used to immunize a different animal species (the immunized animal) using methods known to those of ordinary skill in the art. The immunized animal's humoral immune system reacts to the extracted plasma donor antigens as foreign antigens resulting in xenogeneic antibody production. The xenogeneic antibody-producing animals must be sufficiently taxonomically different from the plasma donor animal so that their immune systems will respond immunologically to the antigens. For example, if the plasma donor is a goat (belonging to the subfamily "Caprinae" of the "Bovidae" family of the "Artiodactyla" order), then the animals used to produce the xenogeneic antibodies have to be members of another family or subfamily e.g. cattle (belonging to the subfamily "Bovinae" of the "Bovidae" family), mouse (order: Rodentia), horse (order: Perissodactyla), rabbit (order: Lagomorpha), etc., but not members of the same subfamily (such as sheep). In one embodiment of the present invention the plasma donor is a human (Homo sapien; family Hominidae; order Primate) and the immunized animal is a goat (Capra sp; species selected from the group including aegagrus, ibex, pyrenaica, cylindricornis, hircus, and falconer i). According to the teachings of the present invention any antigen indigenous to the plasma donor (the "first animal species") may be administered to one or more different animals (the "second animal species") so that xenogeneic antibodies against this antigen(s) are induced. The xenogeneic antibodies may be extracted and combined with the donor plasma from healthy noninfected individuals. The antibody extraction method is not critical and depends on the nature of the xenogeneic antibody preparation that is to be supplied to the donor plasma. If a xenogeneic polyclonal antibody preparation is combined with the donor plasma, then ascites fluid, plasma, plasma fractions, plasma preparations, or serum containing the antibodies of the second group of animal(s) is provided. If a higher grade of xenogeneic antibody purity is desired, immunoglobulin preparations may be obtained by purifying the plasma or ascites fluid using methods known to those in the art, such as, but not limited to immunoprecipitation, gel electrophoresis and affinity chromatography (Arch. Biochem. Biophys. 134, (1969), 279-284; Vox. Sang. 23, (1972), 279-290). Alternatively, xenogeneic monoclonal antibodies may be prepared using methods known to those of ordinary skill in the art (Methods Enzymol. 73, (1981), 3- 46). All methods for providing such xenogeneic (polyclonal and monoclonal) antibody prepai-ations are in principle suited for the present invention. However, reproducibility and consistency of the present invention is enhanced when highly specific and purified xenogeneic antibodies are used. In one embodiment of the present invention, the composition according to the present invention is provided in lyophilized form, which is reconstitutable upon demand but stably stored for long time periods. According to another embodiment of the present invention, the xenogeneic antibody containing plasma is free of inhibitory antibodies of the first group of animals thus reducing the risk that the added xenogeneic antibodies may interfere with indigenous antibodies already present in the donor plasma. The presence of indigenous autoantibodies may be determined in advance using immunoassays known to those of ordinary skill in the art. The compositions according to the present invention are especially suited for testing the efficacy of therapeutics used to treat autoimmune diseases; especially hemophiliacs having inhibitor antibodies. For example, an in vitro method for evaluating auto-antibody disease therapeutic efficacy according to the teachings of the present invention include mixing the autoantibody disease therapeutic with the xenogeneic antibody containing plasma of the present invention to form a mixture. Next, the effect of the autoantibody disease therapeutic on the xenogeneic antibody containing plasma is detected. In one embodiment of the present invention the autoantibody disease therapeutic is an anti-blood clotting factor antibody therapeutic and a blood coagulation assay is used to determine the effects of the xenogeneic antibody containing plasma on the therapeutic. This method is especially suited for evaluating substances for their ability to treat blood coagulation disorders in human patients. FIG. 1 graphically compares the clotting time reduction of human factor VIII deficient plasma that has been treated using Factor Eight Inhibition Bypassing Activity (FEIBA). Curve (a) is the control and depicts the clotting time reduction of human factor VIII deficient plasma containing naturally occurring autoantibodies against factor VIII (patient plasma containing inhibitory antibodies). Curve (b) depicts human factor VIII deficient (immunodepleted) plasma with the xenogeneic antibodies of the present invention added. Finally, curve (c) depicts human normal plasma with the xenogeneic antibodies of the present invention added. The effects of FEIBA is nearly identical in all examples. Therefore, it can be concluded that the xenogeneic antibody containing plasma of the present invention closely simulates plasma having naturally occurring autoantibodies and inhibitory factor. In another embodiment of the present invention xenogeneic antibodies are produced from indigenous antigens derived using recombinant DNA technology as known to those of ordinary skill in the art of molecular biology. Recombinant antigen preparations can be tailored to exacting specifications permitting the production of xenogeneic antibody containing plasma having potencies equal to or greater than naturally occurring inhibitory antibody containing plasma. The present invention is suitable for high throughput routine testing or for single batch control of bioassay reagents which vary in biological activity, concentration and/or composition due to their biological origins. According to another aspect of the present invention, a kit is provided for the evaluation of substances for their ability to be applied to patients suffering from an autoantibody disease, especially inhibitor patients. This kit according to the present invention comprises a composition according to the present invention and a substance to be evaluated. Moreover, such a kit preferably contains reagents for measuring coagulation time as well as standard preparations and reference samples, which may also be provided in lyophilized form. The invention will be described in more detail in the examples and in the figures; however, it is not restricted thereto. Example 1 Preparation of the Xenogeneic Antibody Containing Plasma Using Goat-anti-human Factor VIII (FVIII) A goat is immunized with human recombinant FVIII by the following protocol. Primary immunization by subcutaneous injection of 32.5 μg human recombinant FVIII with complete Freund's adjuvant and two booster injections with 65 μg of protein with incomplete Freund's adjuvant 3 and 8 weeks later. Blood samples are taken in intervals and the anti-FVIII-inhibitor titer is measured with a functional assay (Bethesda assay). Three weeks after the second booster, citrated antiplasma with an inhibitor antibody titer of 6000 Bethesda-Units/ml is obtained by plasmapheresis. The plasma pool is stored frozen in aliquots at < -20°C. For preparation of the xenogeneic antibody containing plasma, the goat-anti- human-FVIII-antiplasma is heated to 56°C for 70 minutes, the resulting insoluble material removed by centrifugation and the supernatant measured with a functional assay (Bethesda-assay) for anti-FVIII antibody titer. Approximately 1.8 ml of the supernatant is mixed with 57 ml of human normal plasma and 6 ml of HEPES/glycine-buffer (10 %(w/v) HEPES, 10 %(w/v) glycine in aqua dest, pH 6.6. The mixture is freeze dried and stored at 2 - 8°C until reconstitution. Example 2 Preparation of the Xenogeneic Antibody Containing Plasma Using Goat-anti-human Factor NIII Goat-anti-human-FVIII-antiplasma is heated to 56°C for 70 minutes, the resulting insoluble material removed by centrifugation and the supernatant measured with a functional assay (Bethesda-assay) for anti-FVIII antibody titer. Approximately 0.3 ml of the supernatant is mixed with 15 ml of FVIII-deficient plasma (immunodepleted). The mixture is freeze dried and stored at 2 - 8°C until reconstitution. Example 3 Preparation of the Xenogeneic Antibody Containing Plasma Using Goat-anti-human Factor VIII Goat-anti-human-FVIII-antiplasma is heated to 56°C for 120 minutes, the resulting insoluble material removed by centrifugation and the supernatant measured with a functional assay (Bethesda-assay) for anti-FVIII titer. Approximately 0.63 ml of the supernatant is mixed with 20 ml of human normal plasma, and 1.5 ml of HEPES/glycine-buffer (10 %(w/v) HEPES, 10 %(w/v) glycine in aqua des , pH 6.6). The mixture is freeze dried and stored at 2 - 8°C until reconstitution. Example 4 Preparation of the Xenogeneic Antibody Containing Plasma Using Goat-anti-human Factor VIII Goat-anti-human-FVIII-antiplasma is subjected to immunoglobulin fractionation according to Kistler et al. (Vox Sang, 1962, 7; 414-424) with some modifications. Briefly, the cryosupernatant is precipitated two times with 20 % ethanol, the resulting precipitate is dissolved and brought to 17 % ethanol. After separation, the supernatant is precipitated with 25 % ethanol, the pellet is dissolved. The resulting immunoglobulin solution contains 12 mg protein/ml and is stored frozen. The immunoglobulin solution (1 ml) is mixed with 13.5 ml immunodepleted FVIII deficient plasma and 1.5 ml HEPES/glycine-buffer (10 %(w/v) HEPES, 10 %(w/v) glycine in aqua dest., pH 6.6) is added. The mixture is freeze dried and stored at 2 - 8 °C. Example 5 Preparation of the Xenogeneic Antibody Containing Plasma Using Goat-anti-human Factor IX (FIX) A goat is immunized with purified human plasma derived FIX by the following protocol. Primary immunization by subcutaneous injection of 90 μg human pd FIX with complete Freund's adjuvant and two booster injections with 90 μg of protein with incomplete Freund's adjuvant 3 and 8 weeks later. Blood samples are taken in intervals and the anti-FIX-inhibitor titer is measured with a functional assay (Bethesda assay). Two weeks after the second booster, citrated antiplasma with an inhibitor antibody titer of 1100 Bethesda-Units/ml is obtained by plasmapheresis. The plasma pool is stored frozen in aliquots at < -20°C. For preparation of the xenogeneic antibody containing plasma, the goat-anti- human-FIX-antiplasma is heated to 56°C for 120 minutes, the resulting insoluble material removed by centrifugation and the supernatant measured with a functional assay (Bethesda-assay) for anti-FIX titer. Two ml of the supernatant is mixed with 34 ml of human normal plasma and 4 ml of HEPES/glycine-buffer (10 %(w/v) HEPES, 10 %(w/v) glycine in aqua dest., pH 6.6. The mixture is freeze dried and stored at 2 - 8°C until reconstitution. Example 6 Preparation of the Xenogeneic Antibody Containing Plasma Using Goat-anti-human von Willebrand factor A goat is immunized with human plasma derived von Willebrand factor. Primary immunization by subcutaneous injection of 2 mg human plasma derived von Willebrand factor with complete Freund's adjuvant and two booster injections with 2 mg of protein with incomplete Freund's adjuvant 4 and 8 weeks later. Blood samples are taken in intervals and the anti-von Willebrand factor-inhibitor titer is measured with an ELISA. One week after the second booster, citrated antiplasma with an inhibitor titer of more than 1:10.000 is obtained by plasmapheresis. The plasma pool is stored frozen in aliquots at < -20°C. For preparation of the xenogeneic antibody containing plasma, the goat-anti- human- von Willebrand factor-antiplasma is heated to 56°C for 70 minutes, the resulting insoluble material removed by centrifugation and the supernatant measured with an ELISA. 10 ml of the supernatant is mixed with 35 ml of human normal plasma and 5 ml of HEPES/glycine-buffer (10 %(w/v) HEPES, 10 %(w/v) glycine in aqua dest., pH 6.6. The mixture is frozen and stored at < -20°C. While the invention has now been described with reference to several embodiments, those skilled in the art will appreciate that various substitutions, omissions, modifications and changes that may be made without departing from the scope or spirit thereof. Accordingly, it is intended that the foregoing description be considered merely exemplary of the invention and not a limitation thereof. Moreover, specific reference has been made to certain scientific publications and/or patents and patent applications, all of which are incorporated herein by reference in their entirety.

Claims: We claim: 1. A xenogeneic antibody containing plasma comprising plasma from a first animal species and antibodies from at least one second animal species wherein said antibodies recognized antigens indigenous to said first animal species. 2. The xenogeneic antibody containing plasma of claim 1 wherein said antigens are selected from group consisting of lipids, phospholipids, glycolipids, proteins, amino acids, glycoproteins, carbohydrates, and nucleic acids. 3. The xenogeneic antibody containing plasma of claim 1 wherein said first animal species is a member of an animal order selected from the group consisting of Artiodactyla, Perissodactyla, Rodentia, Lagomorpha and Primates. 4. The xenogeneic antibody containing plasma of claim 1 wherein said at least one second animal species is a member of an animal order selected from the group consisting of Artiodactyla, Perissodactyla, Rodentia, Lagomorpha and Primates, providing said second animal specific is different from said first animal species. 5. The xenogeneic antibody containing plasma of claim 3 wherein said first animal species is Homo sapien. 6. The xenogeneic antibody containing plasma of claim 4 wherein said second animal species is from the genus Capra. 7. The xenogeneic antibody containing plasma of claim 6 wherein said species of the genus Capra is selected from the group consisting of aegagrus, ibex, pyrenaica, cylindricornis, hircus, and falconeri. 8. The xenogeneic antibody containing plasma of claim 1 wherein said antigen is a blood coagulation factor. 9. The xenogeneic antibody containing plasma of claim 8 wherein said blood coagulation factor is selected from the group consisting of Factor V, Factor VIII, Factor IX, factor X and von Willebrand Factor. 10. Bioassay reagents comprising: plasma derived from a first animal species; and antibodies derived from at least one second animal species that recognized antigens indigenous to said first animal species. 11. The bioassay reagents of claim 10 wherein said antigens are selected from the group consisting of lipids, phospholipids, glycolipids, proteins, amino acids, glycoproteins, carbohydrates, and nucleic acids. 12. The bioassay reagents of claim 10 wherein said antigen is a blood coagulation factor. 13. The bioassay reagents of claim 12 wherein said blood coagulation factor is selected from the group consisting of Factor V, Factor VIII, Factor IX, factor X and von Willebrand Factor. 14. The bioassay reagents of claim 10 wherein said first animal species is selected from an animal order selected from the group consisting of Artiodactyla, Perissodactyla, Rodentia, Lagomorpha and Primates. 15. The bioassay reagents of claim 10 wherein said at least one second animal species is a member of an animal order selected from the group consisting of Artiodactyla, Perissodactyla, Rodentia, Lagomorpha and Primates, providing said second animal specific is different from said first animal species. 16. The xenogeneic antibody containing plasma of claim 14 wherein said first animal species is Homo sapien. 17. The xenogeneic antibody containing plasma of claim 15 wherein said second animal species is from the genus Capra. 18. The xenogeneic antibody containing plasma of claim 17 wherein said species of the genus Capra is selected from the group consisting of aegagrus, ibex, pyrenaica, cylindricornis, hircus, and falconeri. 19. An in vitro method for evaluating autoantibody disease therapeutic efficacy comprising: mixing said autoantibody disease therapeutic with a xenogeneic antibody containing plasma to form a mixture; and detecting the effect of said autoantibody disease therapeutic on said xenogeneic antibody containing plasma. 20. A method according to claim 19 wherein said autoantibody disease therapeutic is an anti-blood clotting factor antibody therapeutic. 21. A method according to claim 19 wherein said detecting step is blood coagulation assay. 22. An in vitro bioassay kit for evaluating autoantibody disease therapeutic efficacy comprising: a first reagent derived from a first animal species plasma mixed with antibodies derived from at least one second animal species that recognized antigens indigenous to said first animal species; and a second reagent that detects the effects of said first reagent on said autoantibody disease therapeutic. 23. The in vitro bioassay kit of claim 22 wherein said second reagent detects, blood coagulation time. 24. The in vitro bioassay kit of claim 22 further comprising analytical standards appropriate for measuring said effect.

Inventors: GRITSCH HERBERT, SCHWARZ HANS-PETER, TURECEK PETER, GRITSCH, HERBERT, SCHWARZ, HANS-PETER, TURECEK, PETER
Applicants: BAXTER AG, BAXTER AKTIENGESELLSCHAFT
Application year: 2001
Main classification: G01N33
IPC codes: G01N33