Method for denaturing allergens

Abstract: Application of a rare earth metal salt can steadily denature the allergens those exist in the environment without any coloring trouble on the treated material.

Description: This invention relates to a method for denaturing allergens in the environment. Furthermore, the present invention relates to a composition for denaturing allergens and to the use of a rare earth metal salt for denaturing allergens. A lot of people suffered from allergic diseases like asthma, atopic dermatitis and so on for long years. Mainly house dust mites that live in the house, hair of pets and various kinds of pollens can be the substances that cause these allergic diseases. Recently, during the treatment of those, medicines are applied to allergic patients. On the other hands, removing allergens which cause allergic disease from the environment where allergic patients live is also a useful method that protects from the exposure to allergens. It is reported in Japan, Europe and the United States of America that removing allergens can improve the condition of patients. Cleaning using a vacuum cleaner or an air conditioner and the use of high-density covers on the bedclothes are applied for removing allergens. However, a vacuum cleaner cannot remove all of the allergens that exist in the house, and an air conditioner can remove allergens that exist only in the atmosphere. Further, high-density cover merely protects allergens inside of bedclothes and is not useful for the allergens that come from the environment. Therefore, these methods are not fully satisfying. Recently the methods for chemically denaturing allergens have been developed. For example, methods that use tannic acid (JP Hei-02-016731A) and extracts of tea leaf, gallic acid and so on are proposed. However, it is hard to get steady effectiveness for denaturing allergens using these proposed methods. Furthermore, these methods have the disadvantage of causing coloring on the materials treated with the proposed denaturing agent. Generally acaricides are used for controlling house dust mites. However, house dust mites, such as Dermatophagoides farinae , Dermatophagoides pteronyssinus , and so on can be the source of allergens even after death and the dead bodies of house dust mites gradually decompose and release fine particles of allergens. As a result, controlling of house dust mites by applying acaricides is not always useful to remove allergens from the environment. US-A-5,900,257 discloses cosmefic/dermatological/phannaceufical compositions, which are said to be suited for the treatment of a variety of special mammalian disorders. The compositions comprise at least one salt of yttrium, lanthanum, cerium, praseodymium, neodymium, promethium, samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium, lutetium, tin, manganese, or mixture thereof, in a cosmetically/pharmaceutically acceptable medium. The present invention was found as the results of earnest efforts to solve these problems, and provides that the use of one or more rare earth metal salts gives excellent denaturing efficacy on allergens without any coloring trouble on the treated material and that the treatment with rare earth metal salt can steadily denature the allergens those exist in the environment. Accordingly, the present invention provides a method for denaturing allergens, which comprises applying an effective amount of a rare earth metal salt to the place where allergens exist. In another embodiment, the present invention provides a composition for denaturing allergens, which comprises rare earth metal salt; at least one polymer selected from polyacrylic acid, polyacrylic acid salts and polycarboxylic surfactants; and at least one selected from water and alcohols. Moreover, the present invention provides the use of a rare earth metal salt for denaturing allergens. Further embodiments of the present application are described in the dependent claims. As the rare earth metal salts of the invention, salts of scandium, yttrium, lanthanum, cerium, praseodymium, neodymium, samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium and lutetium can be used. Among them, the salts of yttrium, lanthanum and cerium are preferably used. These salts can be acetates, nitrates, chlorides, bromides, iodides, carbonates and phosphates, preferably, acetates and chlorides. These rare earth metal salts can be applied as they are. However, they are usually formulated with liquid or solid carrier, and then are applied. The content of rare earth metal salts in these formulations (allergen denaturing compositions) is usually from 0.01 to 50% by weight, preferably from 0.1 to 5% by weight. These allergen denaturing compositions can be in the form of a liquid, powder, paste and so on, and are not restricted if they can denature allergens in the environment. Especially, liquid formulations are effective and easy to handle. One or more kinds of appropriate solvents, which are liquid carriers, can be used for these formulations to dissolve or disperse rare earth metal salts. The solvents are not restricted and for example, hydrophilic solvents such as water, methyl alcohol, ethyl alcohol, isopropyl alcohol, benzyl alcohol, acetic acid, acetone, dimethylformamide, dimethylacetamide, dimethyl sulfoxide, ethylene glycol, propylene glycol, diethylene glycol, triethylene glycol, dipropylene glycol, hexylene glycol, polyethylene glycol, glycerin, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, ethylene glycol monoethyl ether acetate, gamma-butyrolactone, sulfolane and so on, hydrophobic solvents such as dimethylnaphthalene, dodecylbenzene, liquid paraffin, isophorone, kerosene, dibutyl adipate, diethyl phthalate, diethylene glycol monobutyl ether acetate, propylene carbonate, palm oil, rapeseed oil, cottonseed oil, castor oil, soy bean oil and so on. Usually water, alcohols or their mixture are conveniently used. The present allergen denaturing composition which is an acidic liquid formulation is more effective and a formulation containing an organic acid is useful. Examples of the organic acids include citric acid, lactic acid, malic acid, tartaric acid, malonic acid, succinic acid, ascorbic acid, isoascorbic acid, acetic acid, propionic acid, gluconic acid, maleic acid and fumaric acid. Among them, citric acid, lactic acid, malic acid, tartaric acid, ascorbic acid and isoascorbic acid are preferably used. The content of these organic acids is generally from 0.01 to 20% by weight, and preferably from 0.1 to 5% by weight in the formulation. Further, addition of polymer compound containing a carboxyl group is also effective to obtain steady allergen denaturing activity. Polyacrylic acid or its salts, and polycarboxylic surfactants are exemplified as these polymers containing a carboxyl group. Polyacrylic acid or its salts can be co-polymers with maleic acid or vinyl monomers containing sulfonic group and so on. As polycarboxylic surfactants, POISE 520 (KAO Corporation), POISE 530 (KAO Corporation) and DEMOL EP (KAO Corporation) are exemplified. The molecular weight of the polymer is not restricted, and that of more than 2000 is preferable. The content of the polymer containing carboxyl group is usually from 0.01 to 20% by weight, preferably from 0.1 to 5% by weight in the formulation. The present allergen denaturing composition can be any formulation, which is exemplified by aqueous liquid, spray, aerosol, paste and powder. The present allergen denaturing composition can be applied with the other allergen denaturing agents. And for the purpose of removing house dust mite allergens, it is effective to apply the present allergen denaturing composition with acaricides. These acaricides are not restricted if they have lethal activity and/or repellant activity against house dust mites. Examples of the acaricides include benzyl alcohol, benzyl benzoate, phenyl salicylate, cinnamaldehyde, hyssop oil, carrot seed oil, pyrethroid compounds such as natural pyrethrins, phenothrin and permethrin, organophosphate compounds such as fenitrothion, malathion, fenthion and diazinon, dicofol, chlorobenzilate, hexythiazox, tebufenpyrad and pyridaben. The present allergen denaturing composition can also contain a surfactant, chelating agent, anticorrosive agent, antibacterial agent, binder, thickener, perfume, anti-scale agents, antifoaming agents, antistatic agents, softener and so on. The present allergen denaturing composition is applied to an environment, especially indoors. The environment is exemplified by things that contact persons directly, such as carpets, tatami mats, floors, floor covers, bedclothes like futons, sofas, stuffed animals, clothing, curtains, and living spaces in the house, such as wardrobes and closets that keep those things. Generally it is useful for denaturing allergens that the present allergen denaturing composition is directly sprayed onto the carpets, tatami mats, bedclothes, curtains, stuffed animals and so on polluted by allergens. And it is also useful to apply the composition directly to the bedclothes polluted by allergens like starching laundry. Further, it is useful to cover cotton fabrics or nonwoven fabrics applied the compositions on the bedclothes. The application of the present allergen denaturing composition does not cause any coloring troubles. It is desirable to treat acaricides, wash by a washing machine or vacuum out by a vacuum cleaner for highly polluted things by house dust mites. By the present invention, it is possible to denature most of allergens that are originated from mites, hair or epithelium of pets like dogs and cats, cockroaches, feathers, fungi, plant allergens and so on. The mites of allergen include cheyletid mites, grain mites and so on, especially the house dust mites like Dermatophagoides farinae and Dermatophagoides pteronyssinus which are important as the cause of dust mite allergy. Not only these mites themselves, but their bodies and feces also cause strong allergies due to their allergen character. Fungi easily grow in humid places, and cause allergies when swallowed or inhaled into human lungs. Concerning plant allergens, pollens of many kinds of plants, for example, cedar, Japanese cypress, Zelcova serrata, Ambrosia artemisiifoli a , Phleum pratense , Artemisia princeps , Anthoxanthum odaratum , and so on are known. As a result, it is possible to decrease many kinds of allergens practically. Therefore, the invention works effectively on the allergens, particularly on the mite allergens from house dust mites and plant allergens in the environment. EXAMPLES The present invention is more precisely described in the following examples and experiments, and is not restricted by these examples and experiments. Examples 1-18 Each of the components described in Tables 1, 2 and 3 was well mixed to give a homogeneous liquid of the present allergen denaturing composition. Composition of examples Example Nos. 1 2 3 4 5 6 Cerium chloride heptahydrate 2 2 2 2 - 2 Yttrium chloride hexahydrate - - - - 2 - Lactic acid - 2 2 2 2 2 Polyacrylic acid - - 1 1 1 - POISE 520 - - - - - 2 Ethanol 15 15 15 15 15 15 Benzyl alcohol - - - 5 5 5 Water 83 81 80 75 75 74 Composition of examples Example Nos. 7 8 9 10 11 12 Lanthanum chloride heptahydrate 2 2 2 2 - 2 Lanthanum sulfate enneahydrate - - - - 2 - Lactic acid 2 - - - 2 - Citric acid - 2 - - - - Malic acid - - 2 - - - Tartaric acid - - - 2 - - Ascorbic acid - - - - - 2 Polyacrylic acid 1 1 1 1 1 1 Ethanol 15 15 15 15 15 15 Benzyl alcohol 5 5 5 5 5 5 Water 75 75 75 75 75 75 The numbers are % by weight. Composition of examples Example Nos. 13 14 15 16 17 18 Lanthanum chloride heptahydrate 2 - - - - - Gadolinium acetate tetrahydrate - 2 - - - - Samarium chloride hexahydrate - - 2 - - - Dysprosium chloride hexahydrate - - - 2 - - Holmium chloride hexahydrate - - - - 2 - Ytterbium chloride hexahydrate - - - - - 2 Lactic acid 2 2 2 2 2 2 Polyacrylic acid 0.5 0.5 0.5 0.5 0,5 0.5 Ethanol 15 15 15 15 15 15 Water 80.5 80.5 80.5 80.5 80.5 80.5 The numbers are % by weight. To provide for the Experiments below, the reference compositions were prepared by mixing the components described in Table 4. These reference compositions contain salts except rare earth metal salts or an acid. Reference compositions 1-6 Composition of references Reference Example Nos. 1 2 3 4 5 6 Zinc chloride 2 - - - - - Bismuth chloride - 2 - - - - Lithium chloride - - 2 - - - Indium chloride tetrahydrate - - - 2 - - Magnesium chloride hexahydrate - - - - 2 - Gallic acid - - - - - 3 Lactic acid 2 2 2 2 2 - Ethanol 15 15 15 15 15 15 Benzyl alcohol 5 5 5 5 5 - Water 76 76 76 76 76 82 The numbers are % by weight. Experiment 1 Measurement of denaturing efficacy for dust mite allergen Der 2 Approximately 0.03 gram of standard house dust (contains approximately 1000 micrograms/gram of mite Der 2 allergen) was rubbed over the felt (6.6cm in diameter), and the compositions 1 to 18 and reference compositions 1 to 6 were sprayed on the house dust using trigger type spray. After dried in room temperature for 5 hours, the felt was put into a polyethylene bag and the mite allergen of the felt was extracted with 10 ml phosphate buffered saline (pH 7.0, containing 15% by weight of Bovine Serum Albumin) by crumpling for one minute. The extracted solution was centrifuged for 60minutes with 12,000rpm, and the amount of the mite allergen of the supernatant was estimated by the simple detecting kit for the house mite allergen, MITEY CHECKER (product of SHINTO FINE Co., Ltd.). The standard for intensity of the color change for detecting the mite allergen was listed in Table 5. Further, the amount of mite allergen was measured by sandwich method of Enzyme Linked Immunosorbent Assay (ELISA). First, 1000 ng/microliter solution of Derf 2 monoclonal antibody (13A4) was diluted into 500 times, and 100 microliters of the diluted solution was put into F16 MAXISORP NUNC-IMMUNO MODULE plate (product of NUNC company) in each well. Then the plate was coated at 4°C for more than one day. After coating, the solution was removed, and blocking reagent {one percent by weight of Bovine Serum Albumin fraction five (NACALAI TESQUE INC.) with phosphate buffered saline (pH7.2, containing 0.1% by weight of sodium azide)} was added with 100 microliters per well, and reacted at 37°C for 60 minutes. After the reaction, the plate was rinsed with phosphate buffered saline (pH7.2, containing 0.1% by weight of Tween 20 (commercial name) that is a surfactant mainly consisting of polyoxyethylenesorbitan monolaurate produced by Atlas Chemical). Ninety microliters of the supernatant sample and 10 microliters of phosphate buffered saline were added per well, and reacted at 37°C for 60 minutes. Then, Derf 2 monoclonal antibody labeled by peroxidase was diluted into 10 times and 100 microliters per well were added, and the solution was reacted at 37°C for 60 minutes. o-Phenylenediamine (30mg tablet, product of SIGMA CHEMICAL Company) and 15 microliters of 30% hydrogen peroxide were added in 15ml phosphate buffered saline (pH6.2), and 100 microliters of this solution was added into each well of the plate, and reacted at 37°C for 5 minutes. Then, 50 microliters of 2mol/L sulfuric acid was added immediately into each well, and the absorbance was measured using a spectrophotometer for micro-plate (product of Bio-Rad Laboratories Inc.). Blank sample was conducted in the same way except for examples or referenced samples. The results were shown in Table 6. The standard for intensity of the color change for detecting the mite allergen Score Intensity of the color change Amount of mite allergen ++ Thick, apparent line > 35 micro grams (> 350 mites) + Apparent line 10 micro grams (100 mites) ± Slight color change 5 micro grams (50 mites) - No color change < 1 micro gram (<10 mites) Results of denaturing efficacy of the examples for mite allergen Score of Mitey Checker ELISA (microgram) Denaturing ratio (%) Blank ++ 27 - Example 1 ± 5 81 Example 2 - 2 93 Example 3 - 2 93 Example 4 - 1 96 Example 5 - 2 93 Example 6 - 1 96 Example 7 - 1 96 Example 8 - 1 96 Example 9 - 1 96 Example 10 - 1 96 Example 11 - 1 96 Example 12 - 1 96 Example 13 - 1 96 Example 14 - 2 93 Example 15 - 1 96 Example 16 - 2 93 Example 17 - 2 93 Example 18 - 1 96 Reference 1 ++ 20 26 Reference 2 ++ 24 11 Reference 3 ++ 25 7 Reference 4 ++ 23 15 Reference 5 ++ 26 4 Reference 6 ++ 22 19 Experiment 2 Measurement of denaturing efficacy of the example 4 for cedar pollen allergen Cry j 2 Zero, 16, 20 and 24 microliters of the composition obtained in example 4 were reacted with the extract of cedar pollen {5 micrograms per microliter of carbonate-bicarbonate buffered solution (pH 9,5)}, and coated to Linbro/Titertek E.I.A. Microtitration plate (product of ICN BIOCHEMICAL INC.) at 4°C overnight. After coating, the plate was rinsed with phosphate buffered saline (pH7.2, containing 0.1% by weight of Tween 20). Then, cedar pollen antibody Anti-Cry j-2 (Lot. 747032) was diluted into 200 times with phosphate buffered saline (pH7.2, containing one percent by weight of Bovine Serum Albumin and 0.1% by weight of Tween 20), and added with 50 microliters per well, and the solution was reacted at 37°C for 60 minutes. After reacting, the plate was rinsed with phosphate buffered saline (pH7.2, containing 0.1% by weight of Tween 20). HRP-Anti-Rabbit IgG(gamma chain) mouse monoclonal antibody (SIGMA CHEMICAL Company, Lot. 097H4852) was diluted into 5000 times with phosphate buffered saline (pH7.2, containing 0.1% by weight of Tween 20), and added with 50 microliters per well, and of the solution was reacted at 37°C for 60 minutes. After reaction, the plate was rinsed with phosphate buffered saline (pH7.2, containing 0.1% by weight of Tween 20, and then with distilled water, o-Phenylenediamine (30mg tablet, product of SIGMA CHEMICAL Company) and 15 microliters of 30% hydrogen peroxide were added in 15ml phosphate buffered saline (pH6.2), and 100 microliters of this solution was added into each well of the plate, and reacted at 37°C for 15 minutes. Then; 50 microliters of 2mol/L sulfuric acid was added immediately into each well, and the absorbance was measured using a spectrophotometer for micro-plate (product of Bio-Rad Laboratories Inc.). The results were shown in Table 7. Results of denaturing efficacy of example 4 for cedar pollen allergen Cry j2 Added example 4 (micro liter/well) The amount of cedar pollen allergen (absorbance OD490nm) Denaturing ratio (%) 0 0.795 0 16 0.139 82.5 20 0.068 91.4 24 0.059 92.6 Experiment 3 Measurement of denaturing efficacy for dust mite allergen Der 2 by treating on carpet Virgin carpets (50cm X 50cm) were prepared and vacuumed by an electric vacuum cleaner for more than 1 minute. Approximately 50mg of pooled house dust (contains approximately 1mg/g of mite allergen Der 2) were rubbed over each carpet, and the composition obtained in example 4 and 13, reference 5 and 6 were sprayed on the carpet. The sprayed amount was approximately 40g per 1 carpet of each sample, respectively. After being dried at room temperature for 5 hours, house dust on the surface of each carpet was collected using a vacuum cleaner with DUST SAMPLER (product of SHINTO FINE Co., Ltd.) and MITEY-FELT (product of SHINTO FINE Co.,Ltd.) for one minute. Each MITEY-FELT was put into a polyethylene bag and mite allergen of the felt was extracted in the 10ml phosphate buffered saline (pH7.2, containing 10% by weight of Skim milk and 15% by weight of Bovine Serum Albumin) by crumpling for one minute. The extracted solution was centrifuged for 60 minutes with 12,000rpm, and the amount of mite allergen of the supernatant was measured by MITEY CHECKER and the ELISA method. Blank sample was conducted in the same way except for spraying of the examples and/or references. The results were shown in Table 8. Results of denaturing efficacy of the examples for mite allergen Der 2 Score of MITEY CHECKER ELISA (micro gram) Denaturing ratio (%) Blank ++ 47 - Example 7 - 1 98 Example 13 - 2 96 Reference 5 ++ 44 6 Reference 6 ++ 39 17 Experiment 4 Coloring test for cotton fabrics The composition obtained in Example 7 and 3% aqueous solution of tannic acid were treated on the surface of three pieces of cotton fabrics (20cm X 20cm), respectively. The treated amount was approximately 4g per 1 fabric. One of three cotton fabrics was washed using a commercial detergent (ATTACK, product of KAO Corporation), and the other one was exposed to sunshine for 5 hours. Chroma of these fabrics was measured using chroma meter (CT-210, product of MINOLTA Company). The value L* means whiteness and is whitish as near as 100, The value b* means tints and is yellowish as larger than 0 and bluish as less then 0. Results were shown in Table 9. Results of coloring test Blank Example 7 Tannic acid soln. L* (initial) 91.6 91.4 91.0 L* (washed) 91.3 91.1 87.3 L* (exposed) 91.3 91.1 89.8 b* (initial) 0.5 0.2 2.6 b* (washed) -0.1 0.2 5.8 b* (exposed) 0.4 0.4 3.2

Claims: A method for denaturing allergens which comprises applying an effective amount of a rare earth metal salt to the place where allergens exist. The method for denaturing allergens according to claim 1, wherein the rare earth metal salt is a salt of scandium, yttrium, lanthanum, cerium, praseodymium, neodymium, samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium or lutetium. The method for denaturing allergens according to claim 1 or 2, wherein the rare earth metal salt is a salt of yttrium, lanthanum or cerium. The method for denaturing allergens according to claims 1 to 3,wherein the rare earth metal salt is an acetate, nitrate, chloride, bromide, iodide, carbonate or phosphate. The method for denaturing allergens according to claims 1 to 4, wherein the rare earth metal salt is an acetate or chloride. A composition for denaturing allergens, which comprises rare earth metal salt; at least one polymer selected from polyacrylic acid, polyacrylic acid salts and polycarboxylic surfactants; and at least one selected from water and alcohols. The composition according to claim 6, wherein the contents of the rare earth metal salt and the polymer are 0.1 to 5% by weight and 0.01 to 20% by weight respectively. The composition according to claim 6 or 7, further comprising organic acid. The composition according to claim 8, wherein the contents of the rare earth metal salt, the organic acid and the polymer are 0.1 to 5% by weight, 0.01 to 20% by weight and 0.01 to 20% by weight respectively. Use of a rare earth metal salt for denaturing allergens. The use according to claim 10, wherein the rare earth metal salt is a salt of scandium, yttrium, lanthanum, cerium, praseodymium, neodymium, samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium or lutetium. The use according to claim 10 or 11, wherein the rare earth metal salt is an acetate, nitrate, chloride, bromide, iodide, carbonate or phosphate.

Inventors: INUI KEIICHIRO, MIKAME MARIKO, INUI, KEIICHIRO, MIKAME, MARIKO
Applicants: SHINTO FINE CO LTD, SUMITOMO CHEMICAL CO, SHINTO FINE CO., LTD., SUMITOMO CHEMICAL COMPANY, LIMITED
Application year: 2001
Main classification: A01N59
IPC codes: A01N59