FATTY ACID SYNTHASE INHIBITORS

Abstract: This invention relates to the use of compounds as inhibitors of the fatty acid synthase FabH.

Description: FATTY ACID SYNTHASE INHIBITORS FIELD OF THE INVENTION This invention relates to the use of compounds as inhibitors of the fatty acid synthase FabH. BACKGROUND OF THE INVENTION The pathway for the biosynthesis of saturated fatty acids is very similar in prokaryotes and eukaryotes. However, although the chemical reactions may not vary, the organization of the biosynthetic apparatus is very different. Vertebrates and yeasts possess type I fatty acid synthases (FASs) in which all of the enzymatic activities are encoded on one or two polypeptide chains, respectively. The acyl carrier protein (ACP) is an integral part of the complex. In contrast, in most bacterial and plant FASs (type II) each of the reactions are catalyzed by distinct monofunctional enzymes and the ACP is a discrete protein. Mycobacteria are unique in that they possess both type I and II FASs; the former is involved in basic fatty acid biosynthesis whereas the latter is involved in synthesis of complex cell envelope lipids such as mycolic acids. There therefore appears to be considerable potential for selective inhibition of the bacterial systems by broad-spectrum antibacterial agents (Jackowski, S. 1992. In Emerging Targets in Antibacterial and Antifungal Chemotherapy. Ed. J. Sutcliffe & N. Georgopapadakou. Chapman & Hall, New York; Jackowski, S. et al. (1989). J. Biol. Chem. 264, 7624-7629.) The first step in the biosynthetic cycle is the condensation of malonyl-ACP with acetyl-CoA by FabH. In subsequent rounds malonyl-ACP is condensed with the growing-chain acyl- ACP (FabB and FabF, synthesis I and II respectively). The second step in the elongation cycle is ketoester reduction by NADPH-dependent β- ketoacyl-ACP reductase (FabG). Subsequent dehydration by β-hydroxyacyl-ACP dehydrase (either FabA or FabZ) leads to trans-2-enoyl-ACP which is in turn converted to acyl- ACP by NADH-dependent enoyl-ACP reductase (FabI). Further rounds of this cycle, adding two carbon atoms per cycle, eventually lead to palmitoyl-ACP whereupon the cycle is stopped largely due to feedback inhibition of FabH and I by palmitoyl-ACP (Heath, et al, (1996), J.Biol.Chem. 271, 1833-1836). Fab H is therefore a major biosynthetic enzyme, which is also a key regulatory point in the overall synthetic pathway (Heath, R.J. and Rock, CO. 1996. J.Biol.Chem. 271, 1833-1836; Heath, R.J. and Rock, CO. 1996. J.Biol.Chem. 271, 10996- 11000). The antibiotic thiolactomycin has broad-spectrum antibacterial activity both in vivo and in vitro and has been shown to specifically inhibit all three condensing enzymes. It is non-toxic and does not inhibit mammalian FASs (Hayashi, T. et al.,1984. J. Antibiotics 37, 1456-1461; Miyakawa, S. et al., 1982. J. Antibiotics 35, 411-419; Nawata, Y et al., 1989. Acta Cryst. C45, 978-979; Noto, T. et al., 1982. J. Antibiotics 35, 401-410; Oishi, H. et al., 1982. J. Antibiotics 35, 391-396. Similarly, cerulenin is a potent inhibitor of FabB & F and is bactericidal but is toxic to eukaryotes because it competes for the fatty-acyl binding site common to both FAS types (DAgnolo, G. et al., 1973. Biochim. Biophys. Acta. 326, 155-166). Extensive work with these inhibitors has proved that these enzymes are essential for viability. Little work has been carried out in Gram-positive bacteria. There is an unmet need for developing new classes of antibiotic compounds that are not subject to existing resistance mechanisms. No marketed antibiotics are targeted against fatty acid biosynthesis, therefore it is unlikely that novel antibiotics of this type would be rendered inactive by known antibiotic resistance mechanisms. Moreover, this is a potentially broad-spectrum target. Therefore, FabH inhibitors would serve to meet this unmet need. SUMMARY OF THE INVENTION This invention comprises cinnamate derivatives and pharmaceutical compositions containing these compounds and their use as FabH inhibitors that are useful as antibiotics for the treatment of Gram positive and Gram negative bacterial infections. This invention further constitutes a method for treatment of a Gram negative or Gram positive bacterial infection in an animal, including humans, which comprises administering to an animal in need thereof, an effective amount of a compound of this invention. DETAILED DESCRIPTION OF THE INVENTION The compounds of this invention are represented by Formula (I): wherein, R is selected from the group consisting of H, C\.\ Q alkyl, Cj_3 arylalkyl Ci _3 heteroarylalkyl aryl, heteroaryl, Ci _3alkyl-C3_6cycloalkyl, and C3_5cycloalkyl; R2 is selected from the group consisting of H, O(CH2) m aryl, O(CH 2 )mheteroaryl, N(R 5 )(CH 2 ) m aryl, N(R 5 )(CH 2 ) m heteroaryl, N(R 6 )COaryl, N(R6)COheteroaryl, N(Rg)SO2aryl and N(R6)SO2heteroaryl wherein the aryl and hetroaryl moieties of R2and R3 may be optionally substituted by one or more of CH3, CF3, OCF3, OH, OCH3, NH , NHCH3, N(CH 3 ) 2 , SCH3, SOCH3, SO 2 CH 3 , halogen, CO 2 H, CO 2 CH 3 , CONH 2 , CON(CH 3 ) 2 , NHCOH, NHCOCH3, NHSO2CH3, methylenedioxy; provided that R2 is H when R3 is selected from the group consisting of O(CH2) m aryl, O(CH2) m heteroaryl, N(R5)(CH2) m aryl, N(R5)(CH 2 ) m heteroaryl, N(R6)COaryl, N(R6)COheteroaryl, N(R6)SO2aryl and N(R6)SO2heteroaryl; R3 is selected from the group consisting of H, halogen, OCH3, CH3, O(CH 2 ) m aryl, O(CH 2 ) m heteroaryl, N(R 5 )(CH 2 ) m aryl, N(R 5 )(CH 2 ) m heteroaryl, N(R6)COaryl, N(R6)COheteroaryl, N(R6)SO2aryl and N(R6)SO2heteroaryl; provided that R3 is selected from the group consisting of O(CH2) m aryl, O(CH2) m heteroaryl, N(R 5 )(CH 2 ) m aryl, N(R 5 )(CH 2 ) m heteroaryl, N(R 6 )COaryl, N(R6)COheteroaryl, N(R6)Sθ2aryl and N(R6)SO2heteroaryl when R2 and R4 are H; R4 is selected from the group consisting of H, halogen, OCH3, and CH3; R5 is selected from the group consisting of H, CJ.^Q alkyl, Cj_3 alkyl-aryl, Cι_3 alkyl-heteroaryl CO(Cι_g)aιkyl, and COaryl and COheteroaryl; Rg is selected from the group consisting of H, CI^Q alkyl,Cι_3alkyl-aryl and Ci _3alkyl-heteroaryl; and m is an integer from 0 - 3 ; or a pharmaceutically acceptable salt thereof. Also included in the invention are pharmaceutically acceptable salt complexes. As used herein, "Cι_ι Qalkyl" or "alkyl" means both straight and branched chain of 1 to 10 carbon atoms, unless the chain length is otherwise limited, including, but not limited to, methyl, ethyl, rø-propyl, iso-pxopyl, n-butyl, sec-butyl, iso-butyl, tert-butyl, π-pentyl and the like. The alkyl may carry substituents such as hydroxy, carboxy, alkoxy, and the like. The term "cycloalkyl" is used herein to mean cyclic rings, preferably of 3 to 8 carbons, including but not limited to cyclopropyl, cyclopentyl, cyclohexyl, and the like. The term "arylalkyl" or "heteroarylalkyl" or "heterocyclicalkyl" is used herein to mean Cι_ιo alkyl, as defined above, attached to an aryl, heteroaryl or heterocyclic moiety, as also defined herein, unless otherwise indicated. As used herein, "aryl" means phenyl and naphthyl and substituted aryl such as hydroxy, carboxy, halo, alkoxy, methylenedioxy, etc. As used herein, "heteroaryl" means a 5-10 membered aromatic ring system in which one or more rings contain one or more heteroatoms selected from the group consisting of N, O or S, such as, but not limited, to pyrrole, pyrazole, furan, thiophene, quinoline, isoquinoline, quinazolinyl, pyridine, pyrimidine, oxazole, thiazole, thiadiazole, triazole, imidazole, benzotriazole, or benzimidazole. As used herein, preferred aryl substituents include halo, including chloro, fluoro, bromo and iodo, in any combination; Cι_ιoalkyl, C^oalkoxy, aryloxy, or heteroaryloxy. The compounds of this invention may contain one or more asymmetric carbon atoms and may exist in racemic and optically active forms. All of these compounds and diastereomers are contemplated to be within the scope of the present invention. Some of the compounds of this invention may be crystallised or recrystallised from solvents such as organic solvents. In such cases solvates may be formed. This invention includes within its scope stoichiometric solvates including hydrates as well as compounds containing variable amounts of water that may be produced by processes such as lyophilisation. Since the antibiotic compounds of the invention are intended for use in pharmaceutical compositions it will readily be understood that they are each provided in substantially pure form, for example at least 60% pure, more suitably at least 75% pure and preferably at least 85%, especially at least 95% pure, particularly at least 98% pure (% are on a weight for weight basis). Impure preparations of the compounds may be used for preparing the more pure forms used in the pharmaceutical compositions; these less pure preparations of the compounds should contain at least 1%, more suitably at least 5% and preferably from 10 to 49% of a compound of the formula (I) or salt thereof. Preferred compounds of the present invention are selected from the group consisting of: (E)-2'-(6-Chloropiperonyl)-3-(4-phenoxyphenyl)cinnarnic acid; (E)-2'-(3-Bromophenyl)-3-(2,6-dichlorobenzyloxy)cinnamic acid; (E)-3-(2,6-Dichlorobenzyloxy)-2'-[3-(4'-phenoxybiphenyl)]cinnamic acid; (E)-2'-(3-Biphenyl)-3-(2,6-dichlorobenzyloxy)cinnamic acid; (E)-2'-(6-Chloropiperonyl)-3-phenylcinnamic acid; (E)-4-(2,6-Dichlorobenzyloxy)cinnamic acid; (E)-2'-(6-Chloropiperonyl)-4-(2,6-dichlorophenoxy)cinnamic acid; (Z)-2 -(6-Chloropiperonyl)-4-(2,6-dichlorophenoxy)cinnamic acid; (Z)-2 '-(6-Chloropiperonyl)-4-(3 ,5-dichlorophenoxy)cinnamic acid; (E/Z)-2 -Benzyl-4-(2,6-dichlorobenzyloxy)cinnamic acid; (E)-2'-(6-Chloropiperonyl)-3,5-dichloro-4-(2,6-dichlorobenzyloxy)cinnamic acid; (E)-3,5-Dichloro-4-(2,6-dichlorobenzyloxy)-2'-piperonylcinnamic acid; (E/Z)-3-Chloro-4-(2,6-dichlorobenzyloxy)-5-methoxy-2'-piperonylcinnamic acid; (E/Z)-4-(2,6-Dichlorobenzyloxy)-3-methoxy-2'-piperonylcinnamic acid; (E/Z)-4-(2,6-Dichlorobenzyloxy)-3,5-dimethy-2'-piperonylcinnamic acid; (E)-2'-(6-Chloropiperonyl)-4-(3,5-dichloroanilino)cinnamic acid; (E)-2'-(6-Chloropiperonyl)-4-(2,5-dichlorophenoxy)cinnamic acid; (E)-2 , -(6-Chloropiperonyl)-4-(4,6-dimethyl-pyrid-2-ylamino)cinnamic acid; (E)-2'-(6-Chloropiperonyl)-4-(3,4-dichloroanilino)cinnamic acid; (E)-2'-(6-Chloropiperonyl)-4-(3-cyanoanilino)cinnamic acid; (E)-2'-(6-Chloropiperonyl)-4-(N-acetyl-3,4-dichloroanilino)cinnamic acid; (E)-4-(2,6-Dichlorobenzyloxy)-2'-thiophenylcinnamic acid; (E)-2'-(6-Chloropiperonyl)-4-(2,6-dichlorobenzylamino)cinnamic acid; (E)- 4-[Bis(2,6-dichlorobenzy)amino]-2'-(6-chloropiperonyl)cinnamic acid; (E)-2 -(4-Aminobenzyl)-4-(2,6-dichlorobenzyloxy)cinnamic acid; (E)- 4-(2,6-Dichlorobenzyloxy)-2'-(4-methanesulfonaminobenzyl)cinnamic acid; (E)-2'-Cyano-4-(2,6-dichlorobenzyloxy)cinnamic acid; (E)- 4-(3,4-Dichlorobenzyloxy)-2 '-(l-methyltetrazol-5-yl)cinnamic acid; (E)-2'-(2-Cyanoethyl)-4-(2,6-dichlorobenzyloxy)cinnamic acid; (E)-4-(2,6-Dichloro-benzyloxy)]-2'-(4-dimethylaminophenyl)cinnamic acid and (E)-2'-(6-Chloropiperonyl)- 4-(2,6-dichloro-3-hydroxybenzyloxy)cinnamic acid. More preferred compound useful in the present invention are selected from the group consisting of: (E)-2'-(6-Chloropiperonyl)-3-(2,6-dichlorobenzyloxy)cinnamic acid; (E)-2'-(6-Chloropiperonyl)-4-(2,6-dichlorobenzyloxy)cinnamic acid; (E)-2 -(6-Chloropiperonyl)-4-(3,5-dichlorophenoxy)cinnamic acid; (E)-2'-(6-Chloropiperonyl)-4-(2,5-dichloroanilino)cinnamic acid; (E)-2 '-(6-Chloropiperonyl)-4-(3,5-dichlorophenyl)cinnamic acid; (E)-2'-(6-Chloropiperonyl)-4-(2-chloro-5-hydroxybenzyloxy)cinnamic acid; (E)-2'-(Phenyl)-4-(2,6-dichlorobenzyloxy)cinnamic acid; (E)-2 -(6-Chloropiperonyl)-4-(3-amino-2,6-dichlorobenzyloxy)cinnamic acid; (E)- 4-(2,6-Dichlorobenzyloxy)-2 '-(3-thienyl)cinnamic acid and (E)- 4-(2,6-dichloro-3-hydroxybenzyloxy) -2 '-(3-thienyl)cinnamic acid. Compounds of Formula (I) wherein R^ or R^ is benzyloxy are prepared by the methods described in Scheme 1. Scheme 1 a) NaH, DMF; b) NaH, DMF; c) KOH, EtOH; d) acetic acid, piperidine, benzene; e) NaOH, CH3OH, THF, H 2 O Hydroxy benzaldehyde 1 -Scheme- 1, and a benzyl halide (such as 2,6- dichlorobenzyl bromide) are treated with a base (such as sodium hydride) in a solvent (such as DMF) and stirred (6 hours to 30 hours, preferably 16 hours) to yield 3-Scheme-l. Diethyl malonate 5-Scheme-l is alkylated with a benzyl halide (such as 6-chloropiperonyl chloride 4-Scheme-l) and a base (such as sodium hydride) in a solvent (such as DMF) then stirred (1 hours to 10 hours, preferably 3 hours). The resulting diester is mono-saponified using a base (such as potassium hydroxide) in a solvent (such as ethanol) and stirred (4 hours to 30 hours, preferably 18 hours) to yield 6-Scheme-l. Knoevenagel condensation of 3-Scheme-l with 6-Scheme-l in a solvent (such as benzene) with catalysts (such as piperidine and acetic acid) at reflux temperature with azeotropic water removal provides the ester 7-Scheme-l. Saponification of 7-Scheme-l with a base (such as potassium hydroxide) in a solvent (such as ethanol and tetrahydrofuran) provides 8-Scheme-l. Compounds of Formula (I) wherein R^ is phenoxy or aninilo are prepared by the methods described in Scheme 2. Scheme 2 a) acetic acid, piperidine, benzene; b) Cu(Oac)2, TEA, 4A sieves, CH2CI2; c) KOH, EtOH A Knoevenagel condensation of l-Scheme-2 with 2-Scheme-2 (prepared by treating the arylboronic acid with ethylene glycol with concurrent water removal) in a solvent (such as benzene) with catalysts (such as piperidine and acetic acid) at reflux temperature with azeotropic water removal provides the boronic acid 3- Scheme-2. This is coupled with a substituted phenol or aniline (such as 3,5- dichloroaniline) using copper salts (such as Cu(II) acetate) and a base (such as TEA) with molecular sieves in a solvent (such as methylene chloride) to provide 4- Scheme-2. Saponification of 4-Scheme-2 with a base (such as potassium hydroxide) in a solvent (such as ethanol) provides 5-Scheme-2. Compounds of Formula (I) wherein R^ is phenyl are prepared by the methods described in Scheme 3. Scheme 3 a) ArX, (Ph 3 P) 4 Pd(0), Na 2 CO3, toluene, H 2 O; b) KOSi(CH 3 ) 3 , THF A Suzuki coupling of l-Scheme-3 with an aryl halide (such as 3,5- dichloroiodobenzene) in the presence of a palladium catalyst [such as tetrakis(triphenylphosphine)palladium(0)] and a base (such as sodium carbonate) in a solvent (such as toluene and water) provides the ethyl ester of 2-Scheme-3. Saponification of the ethyl ester of 2-Scheme-3 with a base (such as potassium trimethylsilanolate) in a solvent (such as THE) provides 2-Scheme-3. Scheme 4 a) acetic anhydride, TEA A condensation of an arylacetic acid and a 4-benzyloxybenzaldehyde in acetic anhydride with triethyl amine at high temperature provides the 2 - arylcinnamic acids 3-scheme-4. SYNTHETIC EXAMPLES The invention will now be described by reference to the following examples which are merely illustrative and are not to be construed as a limitation of the scope of the present invention. All temperatures are given in degrees centigrade, and all solvents are highest available purity unless otherwise indicated. Example 1 Preparation of (E)-2'-(6-chloropiperonyl)-3-(2,6-dichlorobenzyloxy cinnamic acid a) 3-(2,6-Dichlorobenzyloxy)benzaldehyde To a solution of 3-hydroxybenzaldehyde (3.59 g, 24.39 mmol) and 2,6- dichlorobenzyl bromide (7.05 g, 29.39 mmol) in dimethylformamide (20 mL) at 0°C was added 60% sodium hydride (1.176 g, 29.39 mmol). After stirring at ambient temperature for 16 h, the reaction was partitioned between ethyl acetate and water. The organic layer was washed with aqueous sodium chloride and dried (MgSO/|.). Purification by flash column chromatography (silica gel, hexane/ethyl acetate) yielded the title compound as an off-white solid (7.38g, 89%). b) Diethyl 2-(6-chloropiperonyl)malonate To an anhydrous solution of diethyl malonate (9.44 g, 59 mmol) in DMF (25 mL) stirred at 0°C under argon was added 60% sodium hydride (2.4 g, 60 mmol, oil removed by pentane wash) as a slurry in DMF (10 mL). After stirring 20 min at 0°C, a solution of 6-chloropiperonyl chloride (11.0 g, 53.7 mmol) in DMF (10 mL) was added. The solution was warmed to room temperature and stirred for an additional 3 h. The reaction mixture was partitioned between ethyl acetate and 3N HC1, the organic extract was washed with water then brine and dried (Na2SO4). Removal of solvent in vacuo followed by crystallization from ether / hexanes afforded the title compound (13.14 g, 74.5%). c) 2-(6-Chloropiperonyl)malonic acid monoethyl ester A solution of diethyl 2-(6-chloropiperonyl)malonate (10.7 g, 32.6 mmol) in ethanol (110 mL) with potassium hydroxide (1.84 g, 32.55 mmol) was stirred at room temperature for 18 h. The reaction mixture was diluted with water (100 mL) then concentrated to half the original volume in vacuo. The resulting aqueous solution was washed with ether then acidified with 3N HC1 and the product extracted into ethyl acetate. The organic extract was washed with water then brine and dried (Na2SO4). Removal of solvent in vacuo afforded the title compound (9.84 g, 92%) d) Ethyl (E)-2'-(6-chloropiperonyl)-3-(2,6-dichlorobenzyloxy)cinnamate To a solution of 3-(2,6-dichlorobenzyloxy)benzaldehyde (0.940 g, 3.34 mmol) and 2-(6-chloropiperonyl)malonic acid monoethyl ester (1.00 g, 3.34 mmol) in benzene (10 mL), were added piperidine (26 μL) and glacial acetic acid (40 μL). After refluxing for 10 h with azeotropic water removal, the cooled reaction was diluted with ethyl acetate. The solution was washed successively with 3N hydrochloric acid, aqueous sodium bicarbonate, brine and dried (MgSO4). Purification by flash column chromatography (silica gel, hexane/ethyl acetate) yielded the title compound as a white solid (1.173 g, 67%). e) (E)-2'-(6-Chloropiperonyl)-3-(2,6-dichlorobenzyloxy)cinnamic acid To a solution of ethyl (E)-2 '-(6-chloropiperonyl)-3-(2,6- dichlorobenzyloxy)cinnamic acid (1.17 g, 2.07 mmol) in 1:4 methanol-.tetrahydrofuran (30 mL) was added IN aqueous sodium hydroxide (2.28 mL). After stirring at ambient temperature for 48 h, the reaction was concentrated under reduced pressure. The residue was partitioned between dilute hydrochloric acid and ethyl acetate. The organic layer was washed with aqueous sodium chloride and dried (MgSO4). Trituration with cold ethyl acetate yielded the title compound as a white solid (818 mg, 81%). MS(ES) m e 489.0 [M-H]-. Example 2 Preparation of (E)-2 -(6-Chloropiperonyl)-4-(2.6-dichlorobenzyloxy)cinnamic acid Following the procedure of example 1 (a-e) except substituting 4- hydroxybenzaldehyde for 3-hydroxybenzaldehyde the title compound was prepared as a white solid anal. (C24H17CI3O5) calcd: C, 58.62; H, 3.48; CI, 21.63 found: C, 58.59; H3.26, mp 196-198. Example 3 Preparation of (E)-2 -(6-Chloropiperonyl)-4-(2,5-dichloroanilino)cinnamic acid a) Ethyl (E)-2'-(6-chloropiperonyl)-4-dihydroxyborylcinnamate To a solution of 4-(l,3,2-dioxaborolan-2-yl)benzaldehyde (prepared from 4- dihydroxyborylbenzaldehyde and ethylene glycol in refluxing benzene with azeotropic removal of water) (3.04 g, 17.3 mmol) and 2-(6-chloropiperonyl)malonic acid monoethyl ester (6.5 g, 21.7 mmol) in benzene (75 mL), were added piperidine (86 μL) and glacial acetic acid (247 μL). After refluxing for 23 h with azeotropic water removal, the cooled reaction was diluted with ethyl acetate. The solution was washed successively with 3N hydrochloric acid, water, brine and dried (Na2SO4). Crystallization from ethyl acetate, hexanes afforded the title compound as a white solid (5.8 g, 86%). b) Ethyl (E)-2'-(6-chloropiρeronyl)-4-(2,5-dichloroanilino)cinnamate A slurry consisting of ethyl (E)-2 -(6-chloropiperonyl)-4- dihydroxyborylcinnamate (200 mg, 0.51 mmol), 2,5-dichloroaniline (100 mg, 0.61 mmol) copper(II) acetate (122 mg, 0.61 mmol), triethylamine (430 uL, 3.1 mmol) and 4 A powdered sieves (1.2 g) in methylene chloride (6 mL) was stirred open to air at room temperature for 2 h. The mixture was filtered and all volitiles removed in vacuo. Purification by chromatography (silica gel, hexane/ethyl acetate) provided the title compound as a white solid (142 mg, 46%) c) (E)-2 -(6-Chloropiperonyl)-4-(2,5-dichloroanilino)cinnamic acid A solution of ethyl (E)-2'-(6-chloropiperonyl)-4-(2,5- dichloroanilino)cinnamate (120 mg, 0.24 mmol) and IN aqueous potassium hydroxide (1.5 mL) in acetonitrile (5 mL) was stirred at 50°C for 48 h. The solution was diluted with water and the aqueous solution washed with ether, the aqueous solution was acidified with 3 N HCl and the product extracted into ethyl acetate. The organic extract was washed with water, brine and dried (Na2SO4). Removal of all volatiles in vacuo followed by preparative HPLC purification and crystallization from ethyl acetate / hexane afforded the title compound (57 mg, 50%) mass spectrum ES+: 476.0 [M+H]+. Example 4 Preparation of (E)-2 -(6-Chloropiperonyl)-4-(3,5-dichlorophenoxy)cinnamic acid Following the procedure of example 3 (a-c) except substituting 3,5- dichlorophenol for 3,5-dichloroaniline the title compound was prepared as a white solid mass spectrum ES+: 477.0 [M+H] + Example 5 Preparation of (E)-2 -(6-Chloropiperonyl)-4-(3,5-dichlorophenyl)cinnamic acid a) Ethyl (E)-2'-(6-chloropiperonyl)-4-(3,5-dichlorophenyl)cinnamic acid A mixture consisting of ethyl (E)-2'-(6-chloropiperonyl)-4- dihydroxyborylcinnamate (218 mg, 0.56 mmol), tetrakis(triphenylphosphine)palladium(0) (30 mg, 0.026 mmol), 3,5- dichloroiodobenzene (191 mg, 0.7 mmol) and 2 M aqueous sodium carbonate solution (0.5 mL) in toluene (3 mL) was stirred at 80 - 90°C under argon for 6 h. The reaction mixture was partitioned between ethyl acetate and 3N HCl, the organic extract was washed with water then brine and dried (Na2SO4). Removal of solvent in vacuo afforded the title compound as a crude product, which was used without farther purification in the next step (160 mg). b) (E)-2'-(6-Chloropiperonyl)-4-(3,5-dichlorophenyl)cinnamic acid The above product mixture was stirred in THF (6 mL) with potassium trimethylsilanolate (320 mg, 2.5 mmol) at room temperature for 18 h. The solution was diluted with water and the aqueous solution washed with ether, the aqueous solution was acidified with 3 N HCl and the product extracted into ethyl acetate. The organic extract was washed with water, brine and dried (Na2SO4). Removal of all volatiles in vacuo followed by preparative HPLC purification afforded the title compound (90 mg, 35%) Mass spectrum ES+: 478.2 [M+NH4J+. Example 6 Preparation of (E)-2 -(6-Chloropiperonyl)-4-(2-chloro-5- hvdroxybenzyloxy)cinnamic acid a) tert-Butyl-(4-chloro-3-methyl-phenoxy)-dimethyl-silane Under argon, tert-butyldimethylchlorosilane (1.21 g, 7.71 mmol) was added to a solution of 4-chloro-3-methoxyphenol (1.0 g, 7.01 mmol) and i idazole (1.049 g, 15.42 mmol) in DMF (15 ml) at 0° C. After the addition, the ice bath was removed and the reaction mixture was stirred overnight. The solution was quenched with water and extracted with diethyl ether. The organic extract was washed with 2N HCl, water, aq. NaHCO3, brine then dried ( Na2SO4). Removal of all volatiles in vacuo afforded the title compound as a colorless oil (1.73 g, 96%).Mass spectrum ES+: 257.0 [M+HJ+. b) (3-Bromomethyl-4-chloro-phenoxy)-tert-butyl-dimethyl-silane Benzoyl peroxide (83 mg, 0.34 mmol) was added to a solution of tert-butyl- (4-chloro-3-methyl-phenoxy)-dimethyl-silane (1.73 g, 6.74 mmol) and N- bromosuccinimide (1.32 g. 7.42 mmol) in CCI4 (5 ml) under argon. The reaction mixture was refluxed for 3 h, and then filtered. Removal of volatiles in vacuo and purification by chromatography (silica gel, hexane) afforded the title compound as a colorless oil (1.58g, 70%).Mass spectrum ES+: 336 [M+H]+. c) (E)-2-(6-Chloropiperonyl)- 4-(2-chloro-5-hydroxybenzyloxy)cinnamic acid 4-hydroxybenzaldehyde (152 mg, 1.25 mmol) and (3-Bromomefhyl-4- chloro-phenoxy)-tert-butyl-dimethyl-silane(336 mg, 1.0 mmol) were treated with potassium carbonate (253 mg, 1.875 mmol) in DMF (6 ml) at 60° C for 16 h. The reaction mixture was diluted with water and extracted with ethyl acetate. The organic extract was washed with water, brine and dried over Na2SO4- Removal of solvent and afforded a mixture of 4-[5-(tert-butyl-dimethyl-silanyloxy)-2-chloro- benzyloxyj-benzaldehyde [MS: 377(M+1)] and 4-(2-Chloro-5-hydroxy-benzyloxy)- benzaldehyde[MS: 263(M+1)] which was used without farther purification in the next step. To the above product mixture and 2-(6-chloropiperonyl)malonic acid monoethyl ester (391 mg, 1.3 mmol) in benzene (5 mL), were added piperidine (19 μL) and glacial acetic acid (56 μL). After refluxing for 18 h with azeotropic water removal, the solvent was removed in vacuo to afford a mixture of (E)-2-(6- chloropiperonyl)- 4-(2-chloro-5-hydroxybenzyloxy)cinnamic acid ethyl ester and (E)-2-(6-chloropiperonyl)- 4-[2-chloro-5-(tert-butyl- dimethylsilyloxy)benzyloxy]cinnamic acid. To a solution of this crude mixture in ethanol (3 ml) was added KOH (252 mg, 4.5 mmol). After refluxing for 3 h, the reaction mixture was concentrated under reduced pressure and diluted with water. The aqueous solution was acidified with 3 N HCl and the product extracted into ethyl acetate. The organic layer was washed with water, brine and dried (Na2SO4). Removal of all volatiles in vacuo followed by preparative HPLC purification and crystallization afforded the title compound (75 mg, 16%) as a white cotton-like solid. Mass spectrum ES+: 473.0 [M+H]+. Example 7 Preparation of (E)- 4-(3-Amino-2.6-dichlorobenzyloxy)-2 '-(6- ChloropiperonvDcinnamic acid a) (E)-2'-(6-Chloropiperonyl)-4-hydroxycinnamic acid ethyl ester To 4-hydroxybenzaldehyde ( 1.0 g, 8.18 mmol) and 2-(6- chloropiperonyl)malonic acid monoethyl ester (2.96 g, 9.83 mmol) in benzene (25 ml), were added piperidine (152 μL) and glacial acetic acid (448 μL). After refluxing for 18h with azeotropic water removal, another batch of piperidine (78 μL) and acetic acid (224 μL) was added into the reaction mixture and refluxed for additional 20 h. Removal of volatiles in vacuo followed by crystallization from methanol afforded the title compound as a light yellow solid (1.51 g, 52%). Mass spectrum ES+: 361.0 [M+H]+. b) (E)-4-(3-Amino-2,6-dichloro-benzyloxy)-2'-(6-chloropiperonyl)cinnamic acid To a solution of (E)-2 '-(6-chloropiperonyl)-4-hydroxycinnamic acid ethyl ester (150 mg, 0.42 mmol), 3-amino-2,6-dichlorobenzyl alcohol (121 mg, 0.63 mmol, prepared from 2,6-dichloro-3-nitrobenzoic acid), and triphenylphosphine (219 mg, 0.84 mmol) in THF (3 ml) was added diisopropyl azodicarboxylate (0.84 mmol, 173 μL). After stirring at room temperature for 5h, solvent was removed under reduced pressure. Purification by chromatography (silica gel, hexane/ethyl acetate) afforded a crude product of (E)-4-(3-amino-2,6-dichloro-benzyloxy)-2'-(6- chloropiperonyl)cinnamic acid ethyl ester . Mass spectrum ES+: 534.0 [M+H] + . The above residue was stirred in ethanol (3 ml) with potassium hydroxide(100 mg, 1.78 mmol) at reflux temperature for 3h. The reaction mixture was concentrated under reduced pressure and diluted with water. The aqueous solution was washed with ether and then was acidified with 3 N HCl and the product extracted into ethyl acetate. The organic extract was washed with water, brine and dried (Na2SO4). Removal of all volatiles in vacuo followed by preparative HPLC purification afforded the title compound as a white solid (55 mg, 26%). Mass spectrum ES+: 506 [M+H]+. Example 8 Preparation of (E)- 4-(2.6-Diehlorobenzyloxy) 2 -(3-thienyl)cinnamic acid. A mixture of 4-(2,6-dichlorobenzyloxy)benzaldehyde (250 mg, 0.89 mmol), 3-thiopheneacetic acid (126 mg, 0.89 mmol), triethylamine(0.27 ml) and acetic anhydride(1.36 ml) was heated at 140° C for 16h in a sealed tube. The reaction mixture was quenched with 10% NaOH and stirred for 20 minute, then acidified with 3 N HCl and extracted into ethyl acetate. The organic extract was washed with water, brine and dried (Na2SO4). Removal of all volatiles in vacuo followed by preparative HPLC purification and crystallization afforded the title compound as an off-white crystal (48 mg, 13%). Mass spectrum ES+: 405 [M+H] + . Example 9 Preparation of (E)- 4-(2,6-Dichlorobenzyloxy) 2'-phenylcinnamic acid. Following the procedure of example 8 except substituting phenylacetic acid for 3-thiophineacetic acid the title compound was prepared as a white solid. Mass spectrum ES+: 399 [M+HJ+. Example 10 Preparation of (E)- 4-(2,6-dichloro-3-hydroxybenzyloxy ^'-β-thienvDcinnamic acid. a) (E)-3-(4-Hydroxyphenyl)-2-thiophen-3-ylacrylic acid methyl ester A mixture of 4-hydroxybenzaldehyde (2.44g, 20.0 mmol), 3-thiopheneacetic acid (2.84 g, 20.0 mmol), triethylamine(3.0 ml) and acetic anhydride(15.2ml) was heated at 140° C for 20 h in a sealed tube. After removal of volatiles, the residue was stirred in 10% aqueous NaOH with THE for 1 h, then acidified with 3 N HCl and extracted into ethyl acetate. The organic extract was washed with water, brine and dried (Na2SO4). Removal of all volatiles in vacuo afforded (E)-3-(4-Acetoxy- phenyl)-2-thiophen-3-ylacrylic acid as a brown solid. ES+: 289 [M+H] + . The above residue was stirred in 50 ml of mefhanol with 15 drops of concentrated sulfuric acid at reflux overnight then all volatiles were removed in vacuo and the resulting mixture partitioned between ethyl acetate and water. The organic extract was washed with water then brine and dried over sodium sulfate. Removal of most solvent and filtration of the precipitate afforded the title compound as a gray solid. The mother liquor was further purified by flash chromatography (eluting with 20 % ethyl acetate/hexane) to afford additional product ( 2.93 g, 56.3%). ES+: 261 [M+H]+. b) (E)-3-[4-(2,6-Dichloro-3-hydroxybenzyloxy)phenyl]-2-thiophen-3-ylacrylic acid To an ice-cold solution (E)-3-(4-hydroxy-phenyl)-2-thiophen-3-yl-acrylic acid methyl ester (144 mg, 0.55 mmol) in THF (3 mL) with 2,4-dichloro-3- acetoxybenzyl alcohol (130 mg, 0.55 mmol), and triphenylphosphine (215 mg, 0.825 mmol) was added diisopropyl azodicarboxylate (170uL, 0.825 mmol). The resulting solution was stirred for two days under an argon atmosphere. All volatiles were removed in vacuo and the residue was chromatographed on silica ( eluting with 20 % ethyl acetate/hexane) to afford (E)-3-[4-(3-acetoxy-2,6- dichlorobenzyloxy)phenyl]-2-thiophen-3-yl-acrylic acid methyl ester (176 mg). ES+: 477 [M+H]+. A solution of above product in 1.5 ml of 1,4-dioxane and 1.5 ml of IN aqueous sodium hydroxide (1.5 mL) was stirred at 60°C for 3 h. The solution was diluted with water and acidified with 3 N HCl, and the product extracted into ethyl acetate. The organic extract was washed with water, brine and dried (Na2SO4). Removal of all volatiles in vacuo followed by preparative HPLC purification afforded the title compound (87 mg, 37.5%) as an off white solid. ES+: 421 [M+H]+. Biological Assay: FabH was assayed in a coupled format using his-tagged S.aureus FabD, and acyl carrier protein (ACP) purchased from Sigma. Lyophilized ACP was reduced using β-mercaptoethanol in phosphate buffer. Malonyl-CoA, and FabD were added to the reduced ACP, thus generating malonyl-ACP. After the FabD reaction reached equilibrium, [^C] acetyl-CoA and inhibitors were added, and the reaction started by the addition of FabH. TCA precipitation and filtration was used to separate [^C] acetyl-CoA substrate from [^C] acetoacetyl-ACP product. Secondary and tertiary screens of suitable reproducibility, sensitivity, throughput and analytical power to progress primary screen hits are characterized, validated and in current use. Compounds are evaluated against purified mammalian fatty acid biosynthetic enzymes, E.coli FabH, FabB and a human lung cell cytotoxicity assay. In addition, whole-cell antibacterial activity is determined against a range of clinically relevant wild type and efflux impaired bacteria using standard and novel fluorescence based technologies. The FabH assay has been thoroughly characterized kinetically and a reaction mechanism proposed. Detailed studies have generated novel data about mechanism of inhibition by tool compounds, including thiolactomycin. Screens in use are of direct relevance to the therapeutic goal - eradication of bacteria from sites of infection ('cure'). Several state-of-the-art animal models of bacterial infection are available, meaningful and in current use in this and numerous other studies at SB. Extensive prior experience with known antibacterials confirm that bacterial kill in vitro and in animal models is an excellent indicator of bacterial kill in vivo and cure of infection. The present invention also provides a pharmaceutical composition, which comprises a compound of formula (I) or a pharmaceutically acceptable salt or in vivo hydrolysable ester thereof, and a pharmaceutically acceptable carrier. The compositions of the invention include those in a form adapted for oral, topical or parenteral use and may be used for the treatment of bacterial infection in mammals including humans. The antibiotic compounds according to the invention may be formulated for administration in any convenient way for use in human or veterinary medicine, by analogy with other antibiotics. The composition may be formulated for administration by any route, such as oral, topical or parenteral, especially oral. The compositions may be in the form of tablets, capsules, powders, granules, lozenges, creams or liquid preparations, such as oral or sterile parenteral solutions or suspensions. The topical formulations of the present invention may be presented as, for instance, ointments, creams or lotions, eye ointments and eye or ear drops, impregnated dressings and aerosols, and may contain appropriate conventional additives such as preservatives, solvents to assist drug penetration and emollients in ointments and creams. The formulations may also contain compatible conventional carriers, such as cream or ointment bases and ethanol or oleyl alcohol for lotions. Such carriers may be present as from about 1% up to about 98% of the formulation. More usually they will form up to about 80% of the formulation. Tablets and capsules for oral administration may be in unit dose presentation form, and may contain conventional excipients such as binding agents, for example syrup, acacia, gelatin, sorbitol, tragacanth, or polyvinylpyrollidone; fillers, for example lactose, sugar, maize-starch, calcium phosphate, sorbitol or glycine; tabletting lubricants, for example magnesium stearate, talc, polyethylene glycol or silica; disintegrants, for example potato starch; or acceptable wetting agents such as sodium lauryl sulphate. The tablets may be coated according to methods well known in normal pharmaceutical practice. Oral liquid preparations may be in the form of, for example, aqueous or oily suspensions, solutions, emulsions, syrups or elixirs, or may be presented as a dry product for reconstitution with water or other suitable vehicle before use. Such liquid preparations may contain conventional additives, such as suspending agents, for example sorbitol, methyl cellulose, glucose syrup, gelatin, hydroxy ethyl cellulose, carboxymethyl cellulose, aluminium stearate gel or hydrogenated edible fats, emulsifying agents, for example lecithin, sorbitan monooleate, or acacia; non-aqueous vehicles (which may include edible oils), for example almond oil, oily esters such as glycerine, propylene glycol, or ethyl alcohol; preservatives, for example methyl or propyl p-hydroxybenzoate or sorbic acid, and, if desired, conventional flavouring or colouring agents. Suppositories will contain conventional suppository bases, e.g. cocoa-butter or other glyceride. For parenteral administration, fluid unit dosage forms are prepared utilizing the compound and a sterile vehicle, water being preferred. The compound, depending on the vehicle and concentration used, can be either suspended or dissolved in the vehicle. In preparing solutions the compound can be dissolved in water for injection and filter sterilized before filling into a suitable vial or ampoule and sealing. The solution preferably contains a buffer (such as phosphate) to keep th pH in the range of about 3.5 to 7. DMSO or alcoholic solvents may also be present (at concentrations such as 0.01 to 10 mL/liter) to aid solubility and penetration of the compound of Formula (I) Advantageously, agents such as a local anaesthetic, preservative and buffering agents can be dissolved in the vehicle. To enhance the stability, the composition can be frozen after filling into the vial and the water removed under vacuum. The dry lyophilized powder is then sealed in the vial and an accompanying vial of water for injection may be supplied to reconstitute the liquid prior to use. Parenteral suspensions are prepared in substantially the same manner except that the compound is suspended in the vehicle instead of being dissolved and sterilization cannot be accomplished by filtration. The compound can be sterilized by exposure to ethylene oxide before suspending in the sterile vehicle. Advantageously, a surfactant or wetting agent is included in the composition to facilitate uniform distribution of the compound. The compositions may contain from 0.1% by weight, preferably from 10-60% by weight, of the active material, depending on the method of administration. Where the compositions comprise dosage units, each unit will preferably contain from 50-500 mg of the active ingredient. The dosage as employed for adult human treatment will preferably range from 1 to 140 mg/kg of body weight, depending on the route and frequency of administration.. Inhibitors of β -ketoacyl-ACP Synthase (FabH) can be administered by injection in solutions either intravenously, intramuscularly, intraperitoneally, or orally. The solution preferably contains a buffer (such as phosphate) to keep the pH in the range of about 3.5 to 7. DMSO or alcoholic solvents may also be present (at concentrations such as 0.01 to 10 mL/liter) to aid solubility and penetration of the β- ketoacyl-ACP Synthase (FabH) inhibitor. No unacceptable toxicological effects are expected when a compound of formula (la) or a pharmaceutically acceptable salt or in vivo hydrolysable ester thereof is administered in the above-mentioned dosage range. The compound of formula (I) may be the sole therapeutic agent in the compositions of the invention or a combination with other antibiotics or compounds which enhance the antibacterial activity of a compound of formula (I)may be employed. The antibiotic compounds of the present invention are active against a wide range of organisms including both Gram-negative organisms such as Escherichia coli and Klebsiella pneumoniae and Gram-positive organisms such as Staphylococcus aureus, Streptococcus pneumoniae, Enterococcus faecalis and Enterococcus faecium, including isolates resistant to existing antibiotics. All publications, including but not limited to patents and patent applications, cited in this specification are herein incorporated by reference as if each individual publication were specifically and individually indicated to be incorporated by reference herein as though fully set forth. The above description fully discloses the invention including preferred embodiments thereof. Modifications and improvements of the embodiments specifically disclosed herein are within the scope of the following claims. Without further elaboration, it is believed that one skilled in the area can, using the preceding description, utilize the present invention to its fullest extent. Therefore the Examples herein are to be construed as merely illustrative and not a limitation of the scope of the present invention in any way. The embodiments of the invention in which an exclusive property or privilege is claimed are defined as follows.

Claims: What is claimed is: wherein, R is selected from the group consisting of H, CI _IQ alkyl, Cχ_3 arylalkyl Cι_3 heteroarylalkyl aryl, heteroaryl, Ci _3alkyl-C3_6cycloalkyl, and C3_ cycloalkyl; R2 is selected from the group consisting of H, O(CH2) m aryl, O(CH 2 ) m heteroaryl, N(R5)(CH 2 )maryl, N(R 5 )(CH2) m heteroaryl, N(R 6 )COaryl, N(R6)COheteroaryl, N(R6)Sθ2aryl and N(R6)SO2heteroaryl; provided that R2 is H when R3 is selected from the group consisting of O(CH2) m aryl, O(CH 2 ) m heteroaryl, N(R 5 )(CH 2 )m aryl, N(R 5 )(CH 2 ) m heteroaryl, N(R 6 )COaryl, N(R6)COheteroaryl, N(R6)SO2aryl and N(R6)SO2heteroar l; R3 is selected from the group consisting of H, halogen, OCH3, CH3, O(CH 2 ) m aryl, O(CH 2 ) m heteroaryl, N(R 5 )(CH 2 ) m aryl, N(R 5 )(CH 2 ) m heteroaryl, N(R6)COaryl, N(R 6 )COheteroaryl, N(R6)SO2aryl and N(R6)SO 2 heteroaryl; provided that R3 is selected from the group consisting of O(CH2) aryl, O(CH 2 )mheteroaryl, N(R 5 )(CH 2 ) m aryl, N(R 5 )(CH 2 ) m heteroaryl, N(R 6 )COaryl, N(R6)COheteroaryl, N(R6)S02aryl and N(R6)Sθ2heteroaryl when R2 and R4 are H; R4 is selected from the group consisting of H, halogen, OCH3, and CH3; R5 is selected from the group consisting of H, Cι_ιo alky Cι_3 alkyl-aryl, Ci _3 alkyl-heteroaryl CO(Cι_g)alkyl, and COaryl and COheteroaryl; Rg is selected from the group consisting of H, Cι _ιo alkyl,Cι_3alkyl-aryl and Cι_3alkyl-heteroaryl; and m is an integer from 0 - 3; or a pharmaceutically acceptable salt thereof, or a pharmaceutically acceptable salt complex thereof. 2. A compound according to claim 1 selected from the group consisting of: (E)-2'-(6-Chloropiperonyl)-3-(2,6-dichlorobenzyloxy)cinnamic acid; (E)-2'-(6-ChloiOpiperonyl)-4-(2,6-dichlorobenzyloxy)cinnamic acid; (E)-2'-(6-Chloropiperonyl)-4-(3,5-dichlorophenoxy)cinnamic acid; (E)-2'-(6-Chloropiperonyl)-4-(2,5-dichloroanilino)cinnamic acid; and (E)-2'-(6-Chloropiperonyl)-4-(3,5-dichlorophenyl)cinnamic acid. 3. A method of treating bacterial infections by administering to a patient in need thereof an effective amount of a compound of Formula (I) according to claim 1. 4. A method of treatment according to Claim 1 wherein the compound of Formula (I) is selected from the group consisting of: (E)-2'-(6-Chloropiperonyl)-3-(2,6-dichlorobenzyloxy)cinnamic acid; (E)-2 '-(6-Chloropiperonyl)-4-(2,6-dichlorobenzyloxy)cinnamic acid; (E)-2'-(6-Chloropiperonyl)-4-(3,5-dichlorophenoxy)cinnamic acid; (E)-2 '-(6-Chloropiperonyl)-4-(2,5-dichloroanilino)cinnamic acid; and (E)-2'-(6-Chloropiperonyl)-4-(3,5-dichlorophenyl)cinnamic acid.

Inventors: CHRISTENSEN SIEGFRIED BENJAMIN, DAINES ROBERT A, HEAD MARTHA S, LEBER JACK DALE, LI MEI, WEINSTOCK JOSEPH, CHRISTENSEN, SIEGFRIED, BENJAMIN, IV, DAINES, ROBERT, A., HEAD, MARTHA, S., LEBER, JACK, DALE, LI, MEI, WEINSTOCK, JOSEPH
Applicants: CHRISTENSEN SIEGFRIED B, DAINES ROBERT A, HEAD MARTHA S, LEBER JACK DALE, LI MEI, SMITHKLINE BEECHAM CORP, WEINSTOCK JOSEPH, CHRISTENSEN, SIEGFRIED, BENJAMIN, IV, DAINES, ROBERT, A., HEAD, MARTHA, S., LEBER, JACK, DALE, LI, MEI, SMITHKLINE BEECHAM CORPORATION, WEINSTOCK, JOSEPH
Application year: 2001
Main classification: C07D317
IPC codes: A61K31, A61P31, A61P43, C07D317, C07D333