A COMPOSITION FOR AN INJECTABLE BONE MINERAL SUBSTITUTE MATERIAL

Abstract: The invention refers to an injectable composition for a bone mineral substitute material, which comprises a dry powder mixed with an aqueous liquid. The powder comprises a first reaction component comprising a calcium sulphate hemihydrate with the capability of being hardened to calcium sulphate dihydrate when reacting with said aqueous liquid; a second reaction component, which comprises a calcium phosphate with the capability of being hardened to a calcium phosphate cement when reacting with said aqueous liquid; and at least one accelerator for the reaction of said first and/or second reaction component with said aqueous liquid. A method of producing an injectable bone mineral substitute material is also provided, wherein the composition is mixed in a closed mixing an d delivery system for delivery.

Description: A COMPOSITION FOR AN INJECTABLE BONE MINERAL SUBSTITUTE MATERIAL Technical Field The present invention relates to an injectable composition for a bone mineral substitute material with the capability of being hardened in a body fluid in vivo . Furthermore, the invention relates to a method of producing such a material. Background Art During the last decade, the number of fractures related to osteoporosis, i.e. reduced bone mass and changes in microstructure leading to an increased risk of bone fractures, has almost doubled. Due to the continuously increasing average life time it is estimated that by 2020 people over 60 years of age will represent 25 % of Europe's population and that 40% of all women over 50 years of age will suffer from an osteoporotic fracture. With the aim to reduce or eliminate the need for bone grafting, research has been made to find a suitable artificial bone mineral substitute. Presently, at least the following bone mineral substitutes are used for the healing of bone defects and bone fractures, namely calcium sulphates, as for instance Plaster of Paris, calcium phosphates, as for instance hydroxylapatite, and polymers, as for instance polymethylmetacrylate (P MA) . Calcium sulphate (Plaster of Paris), CaS0 4 • ι/2H 2 0, was one of the first materials investigated as a substitute for bone grafts. Studies have been undertaken since 1892 to demonstrate its acceptance by the tissues and rapid rate of resorbtion. It has been concluded that Plaster of Paris implanted in areas of subperiosteal bone produces no further untoward reaction in the tissue than normally is present in a fracture. Regeneration of bone in the area of subperiosteal resection occurs earlier than when an autogenous graft is used. Plaster of Paris does not stimulate osteogenesis in the absence of bone periosteum. The new bone growing into Plaster of Paris is normal bone. No side effects attributable to the implantation of Plaster of Paris have been noted in adjacent tissues or in distant organs. However, Plaster of Paris has the drawback of very long setting times, which constitutes problems at surgery. Another group of materials for substituting bone tissue in fracture sites and other bone defects is calcium phosphate cements. Due to their biocompatibility and their osteoconductivity they can be used for bone replacement and augmentation. Hydroxylapatite, a crystalline substance which is the primary component of bone, is mainly used as a bone substitute, but is not strong enough for use under weight bearing conditions. Experiments have, shown that hydroxylapatite cement forms a stable implant in respect of shape and volume over 12 months and has the same excellent tissue compatibility as exhibited by commercial ceramic hy- droxylapatite preparations. Microscopic examination clearly demonstrated that hydroxylapatite cement was progressively ingrown by new bone over time. Although the ideal is to achieve hydroxylapatite, there are also apatite-like calcium phosphates which can be obtained as potential bone substitutes. In Table 1 calcium phosphates are presented which are formed by a spontaneous precipitation at room or body temperature, as well as the pH range, within which these components are stable. Table 1 : Calcium phosphates obtained by precipitation at room or body temperature Other calcium phosphates can be obtained by means of sintering at high temperatures, above 1000°C (Table 2) . These calcium phosphates can not be obtained by precipitation in room or body temperature. However, they can be mixed with an aqueous solution alone or in combinations with other calcium phosphates to form a cement-like paste which will set with time. Table 2 : Components forming calcium phosphate cements Bone mineral substitute materials can be used for preparing a paste which can be injected directly into a fracture site. The paste is injected into the void in the bone and, upon hardening, an implant is obtained which conforms to the contours of the gap and supports the cancel- lous bone. Both calcium sulphate and hydroxylapatite materials have been extensively investigated as a possible alternative to autogenous bone grafts to help restore osseous defects of bone and fixation of bone fracture. In this connection it is important that a complete stability is obtained as quickly as possible during or after surgery in order to prevent motions at site of healing. This especially applies to fractures, but also when filling of a bone cavity or replacing bone lost during tumor removal the healing is inhibited by movements and the ingrowth of new bone is prevented. Thus, the injected material must cure fast and adhere firmly to the bone tissue. It is also of importance that the hardened material is so similar in structure to the bone so that it can be gradually resorbed by the body and replaced by new bone growth. This process can be facilitated if the hardened cement is provided with pores, which can transport nutrients and provide growth sites for new bone formation. M. Bohner et al . disclosed at the Sixth World Bio- materials Congress Transactions (15-20/5 2000) a method to obtain an open macroporous calcium phosphate block by using an emulsion of a hydrophobic lipid (oil) in an aqueous calcium phosphate cement paste or an emulsion of an aqueous calcium phosphate cement paste in oil. After setting, the cement block was sintered at 1250° C for 4 hours. Likewise, CN 1193614 shows a porous calcium phosphate bone cement for repairing human hard tissue. The cement contains pore-forming agent which may be a non-toxic surfactant, or a non- toxic slightly soluble salt, acidic salt and alkaline salt. Studies have also been made on mixtures of the above mentioned bone mineral substitute materials. In US-A-4 619 655 is disclosed a bone mineral substitute material comprising a mixture of Plaster of Paris, i.e. calcium sul- phate hemihydrate, and calcium phosphate ceramic particles, preferably composed of hydroxylapatite, or tri- calcium phosphate or mixtures thereof. According to US-A-4 619 655 tests show that when alloplasts composed of 50/50 mixtures of hydroxylapatite/Plaster of Paris were implanted into experimentally created defects in rat mandible, the Plaster of Paris was completely resorbed within a few weeks and replaced by connective tissue. The hydroxylapatite was not resorbed and some particles were eventually completely surrounded by bone. It was therefore concluded that the Plaster of Paris acted as a scaffold for the incorporation of hydroxylapatite into bone. A recent study presented on the "Combined Orthopaedic Research Societies Meeting", September 28-30, 1998, Hamamatsu, Japan, also shows additional tests relating to mixtures of Plaster of Paris and hydroxylapatite. Accord- ing to this study a combination of hydroxylapatite particles and Plaster of Paris had a viscosity which allowed an easy placement of the implant material and prevented migration of hydroxylapatite particles into surrounding tissues during and after implantation. The experiments showed that Plaster of Paris was absorbed in relatively short time, was easily manipulated with hydroxylapatite particles, and did not interfere with the process of bone healing. WO 9100252 shows a composition which is capable of hardening in blood within about 10-45 min. The composition comprises essentially calcium sulphate hemihydrate with small amounts of calcium sulphate dihydrate . Organic and inorganic materials, such as hydroxylapatite, can also be included in the composition. After hardening, particles of hydroxylapatite are obtained within a calcium sulphate cement. The calcium sulphate cement is dissolved rapidly by aqueous body fluids within four weeks, leaving solid particles of hydroxylapatite. Likewise, such particles of hydroxylapatite within a calcium sulphate cement are obtained by the method of WO 9117722. The composition for use as an animal implant comprises calcium sulphate hemihydrate, calcium phosphate, and sodium sulphate. The calcium phosphate is hydroxylapatite and the sodium sulphate enables the composition to be used in the presence of blood or other body fluids . Summary of the Invention The object of the invention is to provide an inject- able composition for a bone mineral substitute material with the capability of being hardened in a body fluid in vivo, which hardens during surgery with accompanying early control of fracture fragment movement as well as provides a stable lasting implant over a year with high mechanical strength, and which during this later period presents a porous as well as irregular structure for bone ingrowth. A further object of the present invention is to provide such an improved injectable bone mineral substitute for filling defects in osteoporotic bone and for additional fracture fixation in substantially cancellous bone which does not exhibit the drawbacks of high viscosity at delivery and low fracture toughness. Still another object of the invention is to provide an injectable bone mineral substitute having excellent biocompatibility, favorable biological and rheological properties. The bone mineral substitute should also be biodegradable and be possible to sterilize by radiation or gas without suffering a significant deterioration in properties. In order to achieve these objects the injectable composition according to the invention has been given the characterizing features of claim 1. According to the invention a composition is provided which comprises two types of bone cement materials, which both are subjected to a hardening reaction in contact with water. A cement of hardened calcium sulphate (gypsum) will remain set in a dry environment. In a wet environment, such as in a Body Simulated Solution, this material will immediately start to disintegrate. Thus, an implanted material with reduced strength will be obtained in the body. The solid material obtained will start to degrade, eventually within 1-2 days. On the other hand, in order to induce a setting (hardening) reaction in a Body Simulated Solution or in a body with its blood, saline can be used. By using saline a setting will be obtained immediately under any conditions, but the implant obtained will still degrade quite rapidly. The second reaction, in which a calcium phosphate is hardened (cemented) to a calcium phosphate cement in the presence of water, will take longer time - about 18 h or more - in order to set to a high strength material . During this period of time the already set sulphate will confer an initial strength to the implant, and when the setting reaction of tricalcium phosphate to a high strength material is completed, a final strength will be obtained, which lasts for months or years . In this connection the term "calcium phosphate cement" refers to the recognized definition (S. E. Gruninger, C. Siew, L. C Chow, A. O'Young, N. K. Tsao, W. E. Brown, J. Dent . Res . 63 (1984) 200) of a reaction product of a powder or a mixture of powders which - after mixing with water or an aqueous solution to a paste - at a temperature around room temperature or body temperature react with the formation of a precipitate, which contains crystals of one or more calcium phosphates and which sets by the entanglement of the crystals within the precipitate. Thus, different calcium phosphate products (calcium phosphate cements) can be obtained during the setting reaction in dependence on the component (s) of the powders used for the paste inventive injectable composition for a bone mineral substitute material . Detailed Description of the Invention The invention will now be explained in more detail, reference being made to the accompanying drawings, in which FIG 1 shows the effects of α-tricalcium phosphate on compressive strength; FIG 2 shows the effects of the content of calcium sulphate dihydrate on the injection time; and FIG 3 shows the effects of the water content and the content of calcium sulphate dihydrate on the setting time. In order to accomplish an injectable bone mineral substitute material having improved characteristics, tests ω ts> t o n o <-n σi in σi 0J rt 1-3 CL T •ϋ S" ø 0 H- rt CD 0 rt CL H rt tr P SD t P- SD Φ φ P- rt 3 Ω Ω Ω P ϋ ø 1 H H 0 P CQ rt P 0 tr Hi H- φ Φ tr φ rt CL CL CD ti fi r H- Φ P ) « PJ CD 0 CD CQ CL CD P J <! 0 3 Ω SD SD 3 Φ SD CL SD SD W J H- C Φ φ TJ P X Ω 3 H ED φ P- CL r P- CD *ϋ P- P- SD t- 1 ^ Ω H p- 1 Φ O Λ ra Hi P- rt M 0 SU rt 0 CQ *< rt rt rt Λ φ — TJ P Mi P- •d CD H- rt W Φ 0 P- rt ^ SD Φ P- CQ P CQ CQ CQ 3 O CD 0 O ii PJ P- ι-3 Q SD H- Φ 0 SD Hi ii tr CL Hi Hi CL O φ 0 ι-3 P φ CQ SD t *. 0 H CΩ Tj rt tr Ω P • Ω 0 Φ Ω Φ Φ Φ r rt P CD 0 tr tr tr rt P- CL — • W , 0 3 rt SD SD 0 0 0 C Ω P H- CL Ω rt SD SD Φ Φ rt SD Φ P CQ Φ CQ rt N φ "b w CO CD rt rt CQ J CQ • Φ rt φ ø 1 P- Ω SD rt t 0 rt CQ rt rt P- (D αi C to P- CD CD r 0 r-> H- ≤ Φ 3 rt CL Φ Φ rt P- p- P- ø ffi i o CD ii rt CL H- f- 1 CQ (ϋ P •0 tr Φ J Φ CL - rt ii P- rt ø rt rt ^ CQ P- o I 0 P- O CD H P O ii rt 0 H- ii tr pu CL P- SD ø- Φ P P- P < ø ≤ rt o 0 0 tr CD CQ P' ) rt SD P , — , ) 0 CQ SD 0 Ω rt SD t- 1 Φ P CQ Λ rt rt ør 1 J rt Ό ^ J CQ S" ii rt CD Φ rt P rt Ω P- h-> P CL rt s.: Φ ø Φ P- rt Ω rr 0 π Pt Ω rt Ω 0 Φ H- 0 Φ SD 0 CQ SD tr Φ tr rt P- rt 3 φ rt P- Ω α ii rr CD - H . tr rt ii 0 M 0 0 rt φ 4 Φ p- CL 3 P- 3 Φ fi tr P- 0 0 H- Ω o 0 CD CQ CQ tr 3 P Ω φ CQ 3 • SD < SD Φ SD ? P ^ P § % 0 rt • Φ rt H- P- 0 >r CL P- P- <! Φ rt φ rt SD Ω -* O CQ P Ω Ω 3 JU P 1 P rt P Hi ø 0 rt P- ^ Φ Φ ø 0 0 -— - o ϊ_» ^^ H P- - P 4 CQ . PJ H- P SD rt H- Φ φ tr 3 0 3 0 Q ii P- fi CL CD 0 CQ SD ø tr ^ ffi h- 1 X CL 0 P ii Φ SD ? SD P- P- Ω -2 3 P- ø μ- rt <_ . o o Λ >. rr Ω Tj ^ Ω rt tr CQ SD CQ ø rt ø ø 0 tr SD SD SD T3 φ Φ + w • > : CD SD J H- ø 3 0 H- Φ φ CQ 0 CQ Φ CQ CQ P- P- φ 0 0 Ω o + H P rt H- d H H- P P Ω rt Φ 3 SD K φ P- Ω P Ω fi rt rt to H SD 0 H- Ω 3 CD Φ l_l. rt rt CQ rt T3 SD CQ P- 0 Ω rt tr Ω rt 0 0 jC TJ Hi tr rt t CD rt φ SD H- P rt tr H SD Hi rt ^ "i 0 SD Hi CQ SD rt + X Ul J CD CD CQ P Ω Ω tr ø σ P- SD H *X3 S CD φ J3 tr P- P 0 o — r Ω Hi P 0 H SD rr H iQ CQ P rt CQ rt tr 0 tr P- SD Ω . — . 0 I- 1 Hi Φ < φ rt CQ Φ SD SD tr Hi 0 >9 * rt rr CL σi TJ • P ii Φ O tr < Φ W + r t- 1 TJ ii Ό CD μ- rt rt H- 3 CQ Ω Φ φ P P- P- Φ SD <! CD Ω SD K 3 H- Ω rt U rt H- rt rt CL 0 rt 0 rt H CQ Ω 0 Ω SD O U) + P- CQ CQ 0 PJ O *< Φ H- 3 P P- p- 0 φ 3 P- tr CL Φ φ 0 P- Hi Ω X tr P CQ « ^ _ - rt l- 1 Hi 3 Φ rt 3 tr 0 T3 p- 0 Φ 3 CQ ø Φ 0 + o x • ; 3 P- " CD Ω 3 Φ Φ φ Hi ^ rr K X rt tr φ *H < rt SD 0 ^ H- SD H SD *ϋ L 0 P- P- 0 3 Φ ø SD O Θ tr Φ rt H o SD Td P- Ii f CL Ω rt rt ^ φ SD H 0 Hi CD 0 SD rt CD 0 φ fi Φ 1 tT CD H H- 3 Ω Φ (D ϋ D ø SD Hi Φ CQ rt rt -3 CL P- rt SD K .5 " 0 C Ω 0 P J Φ H- ii CD H- P CL rt ^ CL. ø- Φ P- 0 ii rt P- P- rt rt • <: CQ J" X • CQ CQ H- 0 Φ φ Hi SD T3 Φ H rt 0 *< P- 0 ø 0 ø CL TJ P- P 4 *<! CL P Φ SD CQ 0-. rt ^ P- t Ω H P- tr o 0 fi Φ H tT 0 Ω h- ' H H h- 1 rt rt ø- P P- SD 0 tr SD P- CQ T3 P φ 0 SD rt P- SU H- ^ PJ T3 p) CD ^ 0 Φ o Φ CD Ω 0 H tf 0 Ω O φ rr 0 P rr - rt CD 0 SD SD H . Ω Φ P Ω ^ Hi rt ø Hi ^ CD 3 CD fu 0 P Hi CQ rt P- CQ ^ P- CL φ CQ σi tr CL 0 u Hi CΩ CQ rt rt CQ rt ii 3 tr ø o H !-J P tr 1 φ H tr rt Φ P- • CL H- Φ CQ φ rt I- 1 Ω φ *> CQ rt 3 co H Hi 0 H SD fl tr Ω H CD rt ø 1 CL p- 0 0 φ ii Φ 0 P w SD φ Φ rt 5 CD Hi CD J L 3 φ P P SD Ω 5 CQ H - o 3 3 CD T3 tr CD H <J H- SD rt CQ CQ C rt X £ D P CL 3 SD P- tr r i H- Ω H- P' ■ : P J H- CL SD o P CQ H H P- rt X 0 0 P- 0 0 Ω CQ P- H- P Ω > J Φ C P- cr CL •d J tr ø Φ φ 0 tT ii CD • ø CD Hi CQ CL tr rt 0 Φ r H Hi t cπ Φ ø ii CL φ Φ Ω CD 0 P CD ø • H φ 0 H p- Φ tr SD 0 SD rt P- 0 SD rt Ό Ω P) P SD 0 SD 0 rt *- ^^ ~~ CD 3 Φ SD 3 3 3 CQ SD fi Tj 0 rt CQ tr 0 C 1 0 Φ P- CQ H P- P- P- 1 P, ω t H CD CQ tT Φ H- CL 0 rt 0 H- 3 SD 1 tr 1 0 1 1 P 5Ca 3 (P0 4 ) 2 + 3H 2 0 → 3Ca s (P0 4 ) 3 OH + H 3 P0 4 (4) 3Ca 4 (P0 4 ) 2 0 + 3H 2 0 → 2Ca 5 (P0 4 ) 3 OH + Ca(OH) 2 (5) Precipitated hydroxylapatite is the least soluble calcium phosphate at pH over 4,2. This means that any other calcium phosphate present in an aqueous solution at this pH range will tend to dissolve, with the precipitation of PHA as a product. This hydrolysis process (Ca(OH) 2 - H 3 P0 4 - H 2 0) is very slow due to a decrease in supersaturation as the reaction proceeds. The only calcium phosphate which can react via a hydrolysis process to an apatite without the formation of sub-products is -tricalcium phosphate (eq. 6) , and the apatite formed in this reaction is a calcium deficient hy- droxylapatite. 3α-Ca 3 (P0 4 ) 2 + H 2 0 → Ca s (HP0 4 ) (P0 4 ) s OH (6) The second group.of reactions to a hydroxylapatite, i.e. precipitated hydroxylapatite (PHA) or calcium deficient hydroxylapatite (CDHA) , is the combinations between TTCP and other calcium phosphates. TTCP is the only calcium phosphate with Ca/P ratio above 1.67. Thus, this substance can be mixed with other calcium phosphates with lower Ca/P ratio to obtain PHA or CDHA without the formation of acids or bases as by-products. Theoretically, any calcium phosphate more acid than PHA can react directly with TTCP to form HA or CDHA according to the following chemical reactions . 7Ca 4 (P0 4 ) 2 0 + 2Ca(H 2 P0 4 ) 2 -H 2 0 → 6Ca 5 (P0 4 ) 3 OH + 3H 2 0 (7) 2Ca 4 (P0 4 ) 2 0 + Ca(H 2 P0 4 ) 2 -H 2 0 → Ca 9 (HP0 4 ) (P0 4 ) 5 OH + 2H 2 0 (8) Ca 4 (PO 4 ) 2 0 + CaHP0 4 -2H 2 0 → Ca s (P0 4 ) 3 OH + 2H 2 0 (9) 3Ca 4 (P0 4 ) 2 0 + 6CaHP0 4 -2H 2 0 → 2Ca 9 (HP0 4 ) (P0 4 ) s OH + 13H 2 0 (10) Ca 4 (P0 4 ) 2 0 + CaHP0 4 → Ca s (P0 4 ) 3 OH (11) 3Ca 4 (P0 4 ) 2 0 + 6CaHP0 4 → 2Ca 9 (HP0 4 ) (P0 4 ) 5 OH + H 2 0 (12) 3Ca 4 (P0 4 ) 2 0 + Ca s H 2 (P0 4 ) 6 -5H 2 0 → 4Ca 5 (P0 4 ) 3 OH + 4H 2 0 (13) 3Ca 4 (P0 4 ) 2 0 + 3Ca 8 H 2 (P0 4 ) 6 -5H 2 0 → 4Ca 9 (HP0 4 ) (P0 4 ) 5 OH + 14H 2 0 (14) Ca 4 (P0 4 ) 2 0 + 2Ca 3 (P0 4 ) 2 + H 2 0 → Ca 5 (P0 4 ) 3 OH (15) In equations (7) and (8) DCPD is formed as an intermediate reaction product, but with PHA or CDHA at the end of the reaction. Reactions (13) , (14) , and (15) are all very slow. However, by using the formulas (9) -(12) it is possible to produce a cement which sets and hardens with time at room or body temperature and at a neutral pH. It is also possible to form PHA as the final hardened product by using mixtures of calcium phosphates with a Ca/P ratio of less than 1.67. This is accomplished by using additional calcium sources, such as Ca(OH) 2 or CaC0 3 , in- stead of TTCP. One example is the reaction β-TCP + DCPD + CaC0 3 → PHA. Initially formed crystals of PHA from a reaction between CDPD and CaC0 3 function as binders between β- TCP particles. When DCPD is consumed the reaction continues between the remaining calcium carbonate and β-TCP with the formation of PHA. However, it seems that the latter process has a detrimental effect on the mechanical strength of the cement . It is preferred that the calcium phosphate with the capability of being hardened to a calcium phosphate cement when reacting with an aqueous liquid is tricalcium phosphate (TCP) , tetracalcium phosphate (TTCP) , anhydrous dicalcium phosphate, monocalcium phosphate monohydrate (MCPM) , dicalcium phosphate dihydrate (DCPD) , or octo- calcium phosphate (OCP) . Preferably, the calcium phosphate is α-tricalcium phosphate. In order to confer an initial strength to a bone mineral substitute material the calcium sulphate hemihydrate in the composition according to the invention should comprise 2-80 wt%, preferably 10-30 wt% of the dry powder to be mixed with an aqueous liquid. Likewise, the calcium ω ts> t >— * o o n © υ> CL rt SD Tj SD rt o CQ H P> Ω Hi ør P Ω 3 SD Hi r P- ι-3 CQ CD rt P- Tj Tj ts CQ Tj P- P- tr f CL 0 • tr Φ in SD 0 SD Φ CQ 0 SD 0 0 tr 0 tr Φ 0 Φ 0 Φ fi 0 tr ø J tr Ω 0 Φ SD P» o CQ o 3 fi rt 3 P- 0 rt CL fi Φ Ω 0 rt H fi fi P- 0 0 ^ P P Hi Tj fi P CQ Ω o φ P- 0 rt Φ 3 fi CD rt Tj μ- rt CQ CL P CQ C rt Φ rt Φ SD p- 1 " R P- fi rt tr CQ H fi ft Φ tr φ μ- tr SD tr CQ φ φ j fi SD fi Φ SD 0 Q- rt 3 P Φ tr Ω *< 0 P- tr CL 0 SD ø SD H Φ fi fi CL tr SD rt ω SD CL Ω CL tr ^ 3 P- SD C H CQ H trJ SD φ CL CQ Z CQ rt t SD tr SD rt Φ o tr rt Ω SD φ CQ fi Hi Φ 0 SD > < Φ tr Φ 3 P- • ; 3 Φ Ω rt φ CQ to 0 P j CD Hi Ω SD Φ Φ rt Hi CQ CQ Φ 3 P- ø rt H 0 Φ • Ω S ^ 0 s: 3 fi 0 Hi fi P- rr CQ CL CL tr ^ P- - ø SD 3 < j Tj 35 0 3 SD TJ P- = > j in φ H P- Φ ø Φ P P- 0 rt J rt SD I- 1 φ fi 0 φ Tj rt SD S rt rt rt H o Hi ts Ω SD 3 Ω Tj tr fi SD tr 0 SD P- Φ rt ø φ φ r fi 0 Ω P- tr tr o\° P- φ tr P- Ω s; ø- SD *< φ φ fi 0 ø fi Φ tr rt Hi CL 0 tr P- H- p- rt SD CD T= H SD Φ rt CD P- H SD H 1 Φ C P- μ- ii Φ P- Φ Φ φ CQ tr P ø tr rt SD 0 Φ 3 J rt 0 P- P SD rt P- Ω P 1 ^ CQ CQ rr SD μ- <J fi H o Φ φ 3 P- ø Hi r φ rt 0 0 P- 0 rt 0 ø - μ- μ- SD 0 Φ SD • Ω SD ø SD tr 0 Tj Ω Ω p- 0 CQ H rt SD tr tr H 0 P> tr CQ SD rt Φ Hi ^ CL fi tr CQ SD p J O ? Φ *d tr CD rf Ω 3 o SD P Ω α> CQ Λ tr rt tr P- Φ SD Φ Ω Φ P ø rt tr 0 μ- « SD 0 ^ SD φ • 6 μ, P Φ S Φ -> tr SD rr rt rt Ω CQ CL CD b-> SD Hi CQ . μ- P- 3 0 0 vo α j p- 3 rt Φ Φ Φ ^ Ω tr φ φ - P- P- SD rt Φ μ- rt φ ø J P CL CQ ∞ Φ tr P P- 0 Ω Φ Tj CD CL rt φ ii H 1 0 p- X 0 φ CO ø fi . Φ 0 CQ P- ø SD p- ø 3 P SD ø SD CQ H p- C SD Φ rt C ø φ CL SD Φ CL CQ P- to rt ξ Φ rr Ω - SD CQ H SD 0 Tj P- Tj i tr CL SD H ø rt r P- 0 P- rf CL φ SD rt Ω o rt rt rt 0 SD ør 1 P- SD Φ SD rt SD tr φ ø CQ tr Φ P- rt CQ 3 0 o\° $. Φ P- • P- tr φ fi • <; CL rt 0 tr SD CQ CL CQ rt Φ ø P- ø rt CL tr ii P- P M Ω SU P- rt CL 0 Tj Φ 0 fi fi rt tr Hi P- 0 j •* 0 P- CQ P- P- Λ 3 P 0 tr co P- fi ^ fi fi SD 0 CL SD Ω fi Ω 0 rt 0 SD Tj tr P- Ω SD P SD SD Ω SD 0 Ω p- fi φ CL SD Φ L S fi P- ii Tj rt H SD ■ ! r tr P- CQ 0 SD H CD 0 rt P- Ω Ω ø 3 0 Φ < 0 Φ 3 SD rt fi tr φ CL CL ø CL rt o tr Φ Φ H- CQ φ Φ <! SD φ CO P- CQ Ω Ω Φ H SD P- Φ φ Hi Ω fi SD tr P- • H Φ o SD rt • CQ φ CL ^ rt rt fi μ- CL Ω Hi φ SD SD rt SD CQ j SD CQ μ- CD SD CQ φ ø P- tr φ P P φ SD μ rt "< p , P- CQ 1-3 ø 1 σ Ω • R Tj P- μ- CQ • CL φ fi rt r P- £ φ SD 3 S P- fi >Q SD Ω φ 0 0 tr SD SD 3 SD ø N CL CD SD P- 0 3 tr CQ S CQ μ- CQ SD SD p tr μ- 0 SD tr Φ rt S H i Φ Φ SD Φ t Ω rt CL P s rt <! ø Tj P- μ- Φ Tj rt ι-3 ø- rt tr φ 0 P- • rt φ r - CL Ω 0 Φ Φ Ω s; P 1 CQ tr ø- tr Φ 0 CD rt SD SD o\° P- SD P- ^ 3 r CQ rt ^> SD fi . SD tr P- 0 μ- Φ CO 0 TJ tr P- Ω tr - P " 0 Ω P- Φ P tr 0 Ω 0 rt CD ø rt fi CQ -J Tj R ii fi 0 Ω φ 3 CL Hi μ- P- P- rt 0 0 rt SD H tr tr Tj Hi H Φ r o tr Φ Φ Φ φ 3 Tj φ ø rt ø tr tr fi Ω Φ φ tr SD SD Φ SD Φ P- 1 0 Ω Hi CQ CL P- ii CD 3 rt iQ SD φ φ j O rt iQ SD CQ 0 Ω rt P- ID O SD Φ tr Φ P- φ P 0 CD ø? cr H SD 0 Hi CQ fi 3 CQ rt tr rt CQ rt ΪS Λ o Tj P" i o 3 H • ; Hi CQ P- Φ l- 1 I- 1 O Ω SD CD SD SD φ φ rt P- φ 0 tr Ω SD -> P- SD CL φ j rt N Φ φ SD Ω fi P- SD CL μ- Ω 0 CQ p- 0 φ P- P- tr CL rt rt fi fi tr Φ j CQ H Ω Φ rt P- ø ø 0 Ω Ω φ ø 0 CL rt rt P t 0 SD SD SD Tj SD CQ SD P- Ω Φ rt P- fi 1 SD r 0 Φ W rt μ- o\° φ 3 ^ CQ P- fi rt tr rt H 0 fi Ω ø P- SD 0 P- φ M SD CL 3 rt 3 Ω o CO - rt ø Φ φ Φ Hi rt rr Ω • P Φ fi 0 CQ μ- φ 0 P- V 0 . tr 0 Ω CQ fi CD ^ Hi p- tr Φ 3 ϋ CL 1 ; ø CQ ø Hi ø 3 in 0 Hi Φ 0 rt Φ H tr S CL φ Ω SD J SD Φ Ω SD φ rt rt CQ SD Tj 0 3 H tr ø in 0 tr tr P- H Φ ø 0 Φ SD CQ rt fi SD CQ Ω tr CL i . SD 0 o SD rt φ j Φ CQ 0 P- Φ ø SD CD H fi fi 0 φ • 0 rt 0 φ Φ Ω rt 0 ø CL tr ø tr rt 3 Ω rt > < tr CD SD H SD rt P 1 C fi 0 $ 0 CQ SD φ CL Φ rt SD Tj tr P- CL tr S CL rt rt P- Tj tu tr Φ SD μ- CQ 3 0 Ω rt SD ts Φ fi Φ rt Φ 3 0 Ω tr *< Φ ø rt rt 0 Ω SD rt H 0 CL Φ H 0 - tr P- Φ SD tr 3 H 0 SD CQ tr tr Hi 5 P- CO 3 H Φ CQ 0 rt SD • 1 rt rt μ- 0 3 1 Φ φ > 0 tr ø 3 H The second reaction of a calcium phosphate to a calcium phosphate cement sets slowly, but can be controlled to set within 18 h as a bone mineral substitute material with a strength of about 30 MPa. This can be accomplished by adding hardened particulate calcium phosphate cement to the inventive composition. The hardened calcium phosphate cement can be hydroxylapatite (HA) , preferably precipitated hydroxylapatite (PHA) , tricalcium phosphate (TCP) , or a mixture thereof . It should have a Ca/P ratio between 1.5 and 2. The particulate calcium phosphate cement should have a particle size which is less than 20 μm, preferably less than 10 μm and comprise between 0.1 and 10 wt%, preferably between 0.5 and 5 wt% of the calcium phosphate which is to react with an aqueous liquid. The reaction of calcium phosphate to a calcium phosphate cement can also be accelerated by a phosphate salt, for example disodium hydrogen phosphate (Na 2 HP0 4 ) , which is dissolved in the aqueous liquid. In this case, the accelerator should be present in the aqueous liquid at concen- trations of 0.1-10 wt%, preferably 1-5 wt%. The two types of accelerator for the reaction of calcium phosphate to calcium phosphate cement can be used either separately or in combination. In the composition according to the invention the aqueous liquid can be distilled water or a balanced salt solution, such as PBS, PBSS, GBSS, EBSS, HBSS, or SBF . The injectability of the composition according to the invention can be improved in several ways. It has surprisingly been shown that a pH reducing component can be added to the inventive composition, the injectability thereof being improved. Such a pH reducing component is for example ascorbic acid or citric acid. These acids are included in the sterile liquid or the sterile powder of the composition in amounts of 0.1-5 wt%, preferably 0.5-2 wt%. Another way to improve the injectability of the composition is to add a biologically compatible oil. The concentration of the oil should be between 0.1 and 5 wt%, preferably between 0.5 and 2 wt%. A suitable oil to be used in the inventive composition is vitamin E. The oil can either be intermixed with the sterile powder or included in the sterile liquid of the composition. As stated above, the addition of a small amount of already reacted calcium sulphate dihydrate had an effect on the injectable time of the bone mineral substitute. Thus, by replacing some of the non-reacted calcium sulphate hemihydrate with reacted calcium sulphate dihydrate, the injectability of the composition could be improved. As much as 95 % of the hemihydrate can be replaced. Preferably, 50-90 % of the hemihydrate is replaced by the dihydrate, most preferred 80-90 %. In order to further improve the bone mineral substitute material obtained with the inventive composition it is possible to further include additional substances, e.g. growth factors, anti-cancer substances, antioxidants and/or antibiotics, etc. Antibiotic containing bone cement is already known and it has been shown that addition of antibiotics to synthetic hydroxylapatite and cancellous bone releases said antibiotics in a concentration suffi- cient for treating bone infections when said substances are administered into the bone. An efficient mixing system must be available in order to prepare the composition according to the invention. The mixing can take place in a conventional cement mixing sys- tem and the composition is injected by means of a convenient delivery system. The mixing container is preferably of that type which can suck the aqueous component into the powder component (German Patent 4409610) . This Prepack™ system is a closed mixing system for delivery in combina- tion with prepacked components in a flexible foil bag. Other mixing devices can of course also be used, for example two interconnected soft bags which can be adapted to a delivering cylinder. The formation of air bubbles in the composition, which can interfere with the hardening reaction of the calcium sulphate hemihydrate and result in a decreased initial mechanical strength of the implanted material during surgery, can be prevented by mixing the composition under conditions of subatmospheric pressure, e.g. in vacuo . How- ever, an atmospheric pressure can also be used. Preferably, the powder component of the composition is sterilized by means of radiation before it is mixed with the sterile liquid component . EXAMPLES The invention will now be further described and illustrated by reference to the following examples. It should be noted, however, that these examples should not be construed as limiting the invention in any way. Compara tive Exampl e 1 . As a control test the injectable time and the setting time of pure calcium sulphate hemihydrate were determined to be more than 10 and 20 minutes, respectively. Comparative Example 2 . As a second control test the injectable time and the setting time of a mixture of calcium sulphate hemihydrate, and hydroxylapatite were also determined to be more than 10 and 20 minutes, respectively. Comparative Example 3 . The injectable time (IT) and the setting time (SI) were studied for the first reaction of a calcium sulphate hemihydrate to calcium sulphate dihydrate in the presence of a passive additive. Twenty different mixtures of calcium sulphate hemihydrate, hydroxylapatite (HA) and accel- erator (Ace) were evaluated, which had different ratios of hydroxylapatite and accelerator, see Table 3. The setting time was determined by a mechanical test . A metallic rod having a weight of 23 g, a diameter of 10 mm and a length of 35 mm was dropped from a height of 35 mm. The time when the rod did not leave any mark on the sample was registered as the setting time. Table 3. Exampl e 1 . Different bi-phasic injectable cements were produced, which were based on α-tricalcium phosphate and -calcium sulphate hemihydrate . The mechanical strength of each cement produced was evaluated with time at 10 hours, 24 hours, 3 days, and 14 days after mixing of the cement with water. The evaluation was performed at the time periods given by means of a cylindrical specimen (d=6mm, h=12mm) that had been immersed in a physiological saline solution of 37°C. The results are shown in Table 4 below. Table 4 Exampl e 2 . The compressive strength was further tested with reference to α-TCP containing less than 20 wt% calcium sulphate hemihydrate (CSH) . (CSH was obtained from Bo Ehrlander AB, Gothenborg, Sweden.) The two powders were mixed together mechanically during 5 min. Then, the liquid was added to the powder at liquid to powder (L/P) ratio of 0.32 ml»g _1 . The liquid contained 2.5 wt% Na 2 HP0 4 as an accelerator. Moulds were then filled and immersed in a saline solution (0,9%) at 37°C for 7 days. The results are shown in Table 5 below and in FIG 1. As seen in FIG 1, the compressive strength was drastivally increased when the α-TCP content exceeded 80 wt%. Table 5 t to ι_. o o <-n comparable to the highest force by hand at which injection was possible. Exampl e 5. The effects of the water content and the content of calcium sulphate dihydrate on the setting time is shown in FIG 3, wherein L/P is the liquid-powder ratio (ml/g) . The setting time was measured by using Gillmore Needles according to ASTM Standard C266. Example 6. In the inventive composition, the form of the calcium sulphate hemihydrate is of importance. α-Calcium sulphate hemihydrate (α-CaS0 4 -ι/2H 2 0) is advantageous to use because of its mechanical strength. α-CaS0 4 -ι/2H 2 0 has a compressive strength of 40.4 MPa compared with 14 MPa for β-α- CaS0 4 -ι/2H 2 0. Exampl e 7. Blodegradation of the Calcium Sulphate wi th Hydroxylapati te bone substi tute in vi tro and in vivo . The degradation rate of calcium sulphate with 40 wt% hydroxylapatite was investigated. The material was placed in a Simulated Body Fluid as well as muscle pockets in rats. The mechanical strength and size of the block obtained were investigated with time as a biodegradation index. Mechanical testing Compressive strength testing was performed using an MTS and Instron 8511.20 testing equipment. After harvesting the materials, the samples were directly placed between self-levelling platens and compressed at 1 mm min -1 until failure at room temperature . Volume measurements After the material harvesting, a caliper measured the volume of the block of material . In vi tro Study Cements of calcium sulphate or calcium sulphate with hydroxylapatite were prepared by mixing with distilled water at L/P ratio of 0.25 ml/g. After mixing the cement was injected into a PFTE mould and allowed to set . The samples were 4 mm in diameter and 8 mm in length. Six cylindrical samples were placed in a Simulated Body Fluid, and the liquid was changed every day. After one week the samples were directly placed between self-levelling platens and sub- jected to compressive strength testing until failure at room temperature . In vivo Study Materials preparation Calcium sulphate hemihydrate (CaS0 4 • ι/2H 2 0) was mixed with 40 wt% hydroxylapatite powder (Ca 10 (Pθ4) 6 (OH) 2 ; HA) . The mixture of POP-HA was sintered and quenched in air. An accelerator (a calcium sulphate) was added at 0.4 wt to the POP-HA, and the dry powder material was sterilized by gamma-irradiation. A cement was prepared by mixing the powder with distilled water at a L/P ratio of 0.25 ml/g. Materials were prepared, which contained calcium sulphate or calcium sulphate + hydroxylapatite. After mixing, the cement was in- jected into a PFTE mould and allowed to set. The samples were cylindrical with diameter of 4 mm and height of 8 mm. Once set, the samples are inserted into muscle pockets of rats . Animals Sprague-Dawley rats weighing around 200 g were used and kept in animal facilities for 1 week before use. The animals were fed a standard laboratory diet. All rats were anesthetized with peritoneal injections of 0.5-0.6 ml of a solution containing 1 ml pentobarbital (60 mg/ml) , 2 ml diazepam (5 mg/ml) , and 1 ml saline (0.15 M) . The implants were inserted in muscles of the rats. Nine rats were used for each period studied. The rats were killed by a peritoneal injection of an overdose of pentobarbital at 1 or 4 weeks after implantation. Results After one week of incubation the mechanical strength was recorded of the cylindrical samples placed in the Simulated Body Fluid or muscles pockets in rats, respectively. The mechanical strength of the materials had decreased from 35 Mpa to about 5 Mpa both in vi tro as well as in vivo. The volume of remaining block was only 1/3 to 1/10 of the original block volume (Table 5) . After 4 weeks of incubation, the mechanical strength of the materials had totally disappeared, and the rods of calcium sulphate were almost completely absorbed. The cal- cium sulphate with hydroxylapatite was still present but totally deformed, and the material was surrounded by normal soft tissue. The tissue also penetrated into the materials. Furthermore, the mass of remaining material was larger than the original block implanted. Table 6 below shows the volume of remaining cylinder material (Mean ±SE) in rat muscles after an incubation of 1 or 4 weeks. The original volume of the cylinder material was 100 mm 3 . Statistic analysis was performed by using the one way ANOVA method and Student's t-test. All results ob- tained exhibited a high statistical significance (p<0.0001) . Table 6. The implanted material comprising calcium sulphate and hydroxylapatite was rapidly degraded within one week in both Simulated Body Fluid and in rats. The rate of degradation was the same in Simulated Body Fluid or muscles pockets, indicating that only one method is needed in order to demonstrate the degradation rate . In conclusion, tests of the combined sulphate and phosphate material exhibit biodegradation in vi tro and in vivo as well as hardening of both components with good results with reference to injectability and setting.

Claims: CLAIMS 1. An injectable composition for a bone mineral substitute material with the capability of being hardened in a body fluid in vivo, which comprises a dry powder mixed with an aqueous liquid, c h a r a c t e r i z e d in that said dry powder comprises a first reaction component comprising a calcium sulphate hemihydrate with the capability of being hardened to cal- cium sulphate dihydrate when reacting with said aqueous liquid; a second reaction component, which comprises a calcium phosphate with the capability of being hardened to a calcium phosphate cement when reacting with said aqueous li- quid; and at least one accelerator for the reaction of said first and/or second reaction component with said aqueous liquid. 2. A composition as in any of claims 1-3, c h a r a c t e r i z e d in that said first and/or said second reaction component is in particulate form with a particle size of 1-100 μm, preferably 1-10 μm. 3. A composition as in claim 1, c h a r a c t e r i z e d in that said calcium sulphate hemihydrate is α- calcium sulphate hemihydrate . 4. A composition as in any of claims 1-3, c a r a c t e r i z e d in that said first reaction component comprises 2-80 wt%, preferably 10-30 wt% of said dry powder . 5. A composition as in claim 1, c h a r a c t e r - i z e d in that said second reaction component is selected from the group comprising tricalcium phosphate (TCP) , tetracalcium phosphate (TTCP) , anhydrous dicalcium phosphate, monocalcium phosphate monohydrate (MCPM) , dicalcium phosphate dihydrate (DCPD) , and octocalcium phosphate (OCP) . 6. A composition as in claim 5, c h a r a c t e r i z e d in that said tricalcium phosphate is a- tricalcium phosphate. 7. A composition as in any of claims 1-2 or 5-6, c h a r a c t e r i z e d in that said second reaction component comprises 10-98 wt%, preferably 70-90 wt% of said dry powder. 8. A composition as in claim 1, c h a r a c t e r i z e d in that said at least one accelerator for the reaction of said first reaction component with said aqueous liquid is particulate calcium sulphate dihydrate. 9. A composition as in claims 8, c h a r a c t e r i z e d in that said particulate calcium sulphate dihydrate is α-calcium sulphate dihydrate. 10. A composition as in claim 8 or 9, c h a r a c t e r i z e d in that said particulate calcium sulphate dihydrate has a particle size of less than 1 mm. 11. A composition as in claim 10, c h a r a c t e r i z e d in that said particulate calcium sulphate dihydrate has a particle size of less than 150 μm, preferably less than 50 μm. 12. A composition as in any of claims 8-11, c h a r a c t e r i z e d in that said particulate calcium sulphate dihydrate comprises between 0.1 and 10 wt%, preferably between 0.1 and 2 wt% of said first reaction component . 13. A composition as in claim 1, c h a r a c t e r i z e d in that said at least one accelerator for the reaction of said second reaction component with said aqueous liquid is particulate calcium phosphate cement. 14. A composition as in claim 13, c h a r a c t e r i z e d in that said particulate calcium phosphate cement has a Ca/P ratio between 1.5 and 2. 15. A composition as in claim 13 or 14 , c a r - a c t e r i z e d in that said particulate calcium phosphate cement is hydroxylapatite (HA) , tricalcium phosphate (TCP), or a mixture thereof. 16. A composition as in claim 15, c h a r a c t e r i z e d in that said hydroxylapatite is precipit- ated hydroxylapatite (PHA) . 17. A composition as in any of claims 13-16, c h a r a c t e r i z e d in that said particulate calcium phosphate cement has a particle size which is less than 20 μm, preferably less than 10 μm. 18. A composition as in any of claims 13-17, c h a r a c t e r i z e d in that said particulate calcium phosphate cement comprises between 0.1 and 10 wt%, preferably between 0.5 and 5 wt% of said second reaction component . 19. A composition as in claim 1, c h a r a c t e r i z e d in that said aqueous liquid comprises destilled water or a balanced salt solution. 20. A composition 'as in claim 1 or 19, , c h a r a c t e r i z e d in that said at least one accelerator for the reaction of said second component with said aqueous liquid is dissolved in said aqueous liquid. 21. A composition as in claim 20, c h a r a c t e r i z e d in that said accelerator is disodium hydrogen phosphate (Na 2 HP0 4 ) . 22. A composition as in claim 20 or 21, , c h a r a c t e r i z e d in that said accelerator comprises 0.1-10 wt%, preferably 1-5 wt% of said aqueous liquid. 23. A composition as in claim 1 or 19, c h a r a c t e r i z e d in that said aqueous liquid comprises between 0.1 and 2 ml, preferably between 0.5 and 1 ml per gram of said powder. 24. A composition as in any of claims 1-23, c h a r a c t e r i z e d in that up to 95 %, preferably between 80 and 90 %, of said calcium sulphate hemihydrate is replaced by hardened calcium sulphate dihydrate in order to improve the injectability thereof. 25. A composition as in any of claims 1-23, c h a r a c t e r i z e d in that it further comprises a biologically compatible oil in order to improve the injectability thereof. 26. A composition as in claim 1, c h a r a c t e r i z e d in that said biologically compatible oil is vitamin E. 27. A composition as in claim 26 or 27, , c h a r a c t e r i z e d in that said biologically compatible oil comprises between 0.1 and 5 wt%, preferably between 0.5 and 2 wt% . 28. A composition as in any of claims 1-23, c h a r a c t e r i z e d in that it further comprises a pH reducing component in order to improve the injectability thereof. 29. A composition as in claim 28, c h a r a c t e r i z e d in that said a pH reducing component is ascorbic acid or citric acid. 30. A composition as in claim 28 or 97, , c h a r a c t e r i z e d in that said pH reducing component comprises between 0.1 and 5 wt%, preferably between 0.5 and 2 wt%. 31. A composition as in claim 1, c h a r a c t e r i z e d in that said dry powder is sterile. 32. A composition as in claim 1, c h a r a c t e r i z e d in that it further comprises biologically active substances, such as growth factors and/or anti-cancer substances and/or antibiotics and/or antioxidants . 33. Method of producing an injectable bone mineral substitute material, wherein a composition as in any of claims 1-32 is mixed in a closed mixing and delivery system, preferably under conditions of subatmospheric pres- sure .

Inventors: LIDGREN LARS, LIDGREN, LARS
Applicants: BONE SUPPORT AB, LIDGREN LARS, LIDGREN, LARS
Application year: 2001
Main classification: A61L27
IPC codes: A61L24, A61L27